[Histonet] RE: Anti-human nuclear antibody

[Anatoli Gleiberman]

Elizabeth,
We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03),

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron
Sent: Thursday, April 11, 2013 11:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Anti-human nuclear antibody

Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol?  We are trying to detect human cells in mice.
Thank you.

Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine



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