[Histonet] RE: compression problems

[Elizabeth Chlipala]

Denise

We use paraplast and paraplast extra and have not experienced any problems.  We just got a new lot of paraplast extra in and so far everything seems ok.  I would check to see if it's a particular lot number that you are using.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Denise G Crowley
Sent: Thursday, April 11, 2013 7:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] compression problems

Hi all,

Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months?  We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists.  This involves all 4 of our microtomes to varying degrees and all types of tissue.  The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes.  We use Paraplast Xtra for both infiltrating and embedding.
Any suggestions?

Denise Crowley
Hope Babette Tang Histology Facility
Koch Center for Integrative Cancer Research Massachusetts Institute of Technology
500 Main St. 76-182
Cambridge MA 02139
617-258-8183
dencrowl <@t> mit.edu<mailto:dencrowl <@t> mit.edu>

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