[Histonet] IHC (DAB) after H&E?

[Vanessa Perez]

Same here, we just run down the HE slide back to water and load, the running back to water takes out some of the eosin/hematoxylin in the process....

Vanessa 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of MARY T HODGES 
Sent: Friday, April 05, 2013 8:44 AM
To: Rene J Buesa ; NMargaryan <@t> luriechildrens.org ; histonet <@t> lists.utsouthwestern.edu ; histonet-bounces <@t> lists.utsouthwestern.edu ; histonet-request <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] IHC (DAB) after H&E?

I have just ihc over the section and not removed the h/e as long as it is on a positive charged slide and had no problems Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Date: Fri, 5 Apr 2013 12:00:40
To: <NMargaryan <@t> luriechildrens.org>; <histonet <@t> lists.utsouthwestern.edu>; <histonet-bounces <@t> lists.utsouthwestern.edu>; <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC (DAB) after H&E?


Yes, you need to remove it. Hydrate your sections and place them in a 1% aq. acetic acid solution (it will be a weak acid solution) and you will see how the eosin fades and the hematoxylin becomes reddish. Keep them in the solution until almost all the hematoxylin becomes less evident but it will never totally "disappear". This will be of minor importance unless the epitope you are trying to detect is nuclear.
 
  René J.
 
 From: "Margaryan, Naira" <NMargaryan <@t> luriechildrens.org>
 To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "histonet-bounces <@t> lists.utsouthwestern.edu" <histonet-bounces <@t> lists.utsouthwestern.edu>; "histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu> 
 Sent: Thursday, April 4, 2013 5:23 PM
 Subject: [Histonet] IHC (DAB) after H&E?
 
 Dear Histonetters,
 
 I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that?
 
 Thanks in advance,
 Naira
 
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