[Histonet] Nail treatment and bone marrow decalcification info needed

[Rene J Buesa]

Use 10% aq. sol. of sodium or potassium hydroxide for 30 minutes on already fixed toe (or finger) nails, wash thoroughly, and process as usual.

Decalcify BM with EDTA overnight
René J.

From: carmen loiselle <carmen_loiselle <@t> hotmail.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, April 3, 2013 4:44 PM
Subject: [Histonet] Nail treatment and bone marrow decalcification info needed

Hello fellow histonetters ,

I need your expertise regarding the 
treatment of nails.  We currently immerse the nails specimen in a 
solution of tween & formalin up to 2 weeks .  We then process and 
section them.  Is there a more rapid and efficient way to do it ???


Also, 
we recently change our procedure for the fixation of the bone marrow 
specimen.  Up to last week we were using Zenker's solution .  Starting 
in april we are now using the formalin fixation for 24 hours before 
decalcification.  What are the most efficient and rapid way of doing 
the decalcification without altering the IHC markers  currently use for 
these type of specimen ???
At the present time we are using 10% 
formic acid for 2 hours, rinse then process.  What about RDO agent  
(which I believe is HCL based)  which is faster ????

Thank you in advance for all your responses

Carmen                         _______________________________________________
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