[Histonet] RE: Cell block preparation

[Tom McNemar]

We used to do this using culture slants from micro.  I still do it sometimes if I don't have much material.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Monday, April 01, 2013 7:50 AM
To: Dessoye, Michael J; Histonet
Subject: [Histonet] RE: Cell block preparation

We use Histogel.  The cell block material is spun down and the supernate is poured off.  The Histogel tube is liquefied using the microwave.  Equal portions (cell concentration and Histogel) are then resuspended.  Let this solidify, then slice (if needed) and place in a cassette for processing.



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J
Sent: Friday, March 29, 2013 12:41 PM
To: Histonet
Subject: [Histonet] Cell block preparation

Hello Histonet,

What is everyone doing regarding cell block preparation?  We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids).  We will be testing sponges, biopsy bags, and paper...
anyone have a method they prefer?

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

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