From DKBoyd <@t> chs.net Mon Apr 1 06:49:56 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Mon Apr 1 06:50:08 2013 Subject: [Histonet] RE: Cell block preparation In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246794D16DE@TN001WEXMBX12.US.chs.net> We use Histogel. The cell block material is spun down and the supernate is poured off. The Histogel tube is liquefied using the microwave. Equal portions (cell concentration and Histogel) are then resuspended. Let this solidify, then slice (if needed) and place in a cassette for processing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, March 29, 2013 12:41 PM To: Histonet Subject: [Histonet] Cell block preparation Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From HParker <@t> Skaggs.Net Mon Apr 1 08:16:19 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Mon Apr 1 08:16:23 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 33 In-Reply-To: <5694485b-3d55-46ec-8630-e315bf61be43@email1.skaggs.net> References: <5694485b-3d55-46ec-8630-e315bf61be43@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300D6F@email1.skaggs.net> Hi Michael, I have always wrapped the cell blocks in lens paper. If it is a messy one I wrap it 2 or 3 times in lens paper. Helayne, <> _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From TMcNemar <@t> lmhealth.org Mon Apr 1 08:22:37 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Apr 1 08:21:45 2013 Subject: [Histonet] RE: Cell block preparation In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246794D16DE@TN001WEXMBX12.US.chs.net> References: <7EAFE982E328304DA6CE2B677BB76246794D16DE@TN001WEXMBX12.US.chs.net> Message-ID: We used to do this using culture slants from micro. I still do it sometimes if I don't have much material. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Monday, April 01, 2013 7:50 AM To: Dessoye, Michael J; Histonet Subject: [Histonet] RE: Cell block preparation We use Histogel. The cell block material is spun down and the supernate is poured off. The Histogel tube is liquefied using the microwave. Equal portions (cell concentration and Histogel) are then resuspended. Let this solidify, then slice (if needed) and place in a cassette for processing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, March 29, 2013 12:41 PM To: Histonet Subject: [Histonet] Cell block preparation Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Sadeq.Kharzai <@t> nationwidechildrens.org Mon Apr 1 09:34:02 2013 From: Sadeq.Kharzai <@t> nationwidechildrens.org (Kharzai, Sadeq) Date: Mon Apr 1 09:34:12 2013 Subject: [Histonet] (no subject) Message-ID: Please remove me from the list. Thanks, Sadeq Kharzai From mhale <@t> MiracaLS.com Mon Apr 1 09:55:29 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Mon Apr 1 09:55:40 2013 Subject: [Histonet] KY Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C720339D@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From jpiche <@t> wtbyhosp.org Mon Apr 1 11:27:36 2013 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Mon Apr 1 11:27:40 2013 Subject: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation Message-ID: <631955447A364B45B9458D2905635110655E9470@WIN08-MBX-01.wtbyhosp.org> From: Piche, Jessica Sent: Monday, April 01, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: ANP.23120 Tissue Processing schedules needing validation Hello Everyone, For ANP23120 Tissue processing schedules are validated: we have two processors that we have no true validation for. Are we expected to do this retroactively? Can anyone share one of their procedures for validation of new schedules for tissue processors?? Also what does WC mean? It also says they want WC records documenting validation. Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From vperez <@t> pathreflab.com Mon Apr 1 12:12:04 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Mon Apr 1 12:12:15 2013 Subject: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation Message-ID: Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: Vanessa Perez Sent: Monday, April 01, 2013 12:12 PM To: 'Piche, Jessica' Subject: RE: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation We just made a procedure for future adjustments to processing schedule. Did not have to do any retroactive validations, Just for any new changes to processing schedule. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Monday, April 01, 2013 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation From: Piche, Jessica Sent: Monday, April 01, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: ANP.23120 Tissue Processing schedules needing validation Hello Everyone, For ANP23120 Tissue processing schedules are validated: we have two processors that we have no true validation for. Are we expected to do this retroactively? Can anyone share one of their procedures for validation of new schedules for tissue processors?? Also what does WC mean? It also says they want WC records documenting validation. Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Apr 1 12:27:05 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Apr 1 12:27:10 2013 Subject: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation In-Reply-To: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C15@isexstore03> We did the same,as well as making up a form to be used, to include 1) name of program 2) Date of test run for validation 3) Tissue types used (10) 4) Date of assessment and validation 5) date program authorized for use and 6) the Pathologist's signature. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, April 01, 2013 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: Vanessa Perez Sent: Monday, April 01, 2013 12:12 PM To: 'Piche, Jessica' Subject: RE: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation We just made a procedure for future adjustments to processing schedule. Did not have to do any retroactive validations, Just for any new changes to processing schedule. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Monday, April 01, 2013 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: ANP.23120 Tissue Processing schedules needing validation From: Piche, Jessica Sent: Monday, April 01, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: ANP.23120 Tissue Processing schedules needing validation Hello Everyone, For ANP23120 Tissue processing schedules are validated: we have two processors that we have no true validation for. Are we expected to do this retroactively? Can anyone share one of their procedures for validation of new schedules for tissue processors?? Also what does WC mean? It also says they want WC records documenting validation. Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From BZIMMERM <@t> gru.edu Mon Apr 1 15:13:45 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Apr 1 15:13:52 2013 Subject: [Histonet] Happy Moanday Everyone!! from Billie Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF760926@EX-MLB-03.ad.georgiahealth.edu> By now, even the procrastinators should have a room for the GSH symposium. If not, please contact Linda Schepps at Oceanside. Linda will be able to find you a room at our symposium rate. Remember that Jekyll Island is a state park with a wide range of opportunities for fun. As you enter the island, you will pay a flat rate of $6 and on the back of the receipt will be a coupon that can be used on the island. I know firsthand that the machine takes $10 bills for entrance to the island. It was windy that day and I lost a dollar bill in the wind. I had a fleeting thought of jumping out of the car like a crazy woman, and chasing that dollar bill. But then my brain said, are you going to risk your own life for $1?? What would they have said about that at my eulogy?? If you don't plan on leaving the island for the duration of the symposium that will be your one time charge. There's really no reason to leave the island because you will be busy and at the Hotel California-you can check in any time you like but you can never leave....just kidding!! There's horseback riding on the beach (this gal will not be doing it) tennis, golf on a historic course, goofy golf, bicycling, and swimming in the pool which is on the beach side of the hotel. There will be sunrise beach walks (yea, right) looking for sand dollars. Also, you will see ginormous, badonka jelly fish on the beach. If you have any questions or concerns, please contact me, Billie Zimmerman, your social director, who gives you the answer without the fluff, sprinkles, or candy coating. Wanda Simons, our GSH president, wrote this paragraph but I did an aggressive takeover and changed it up a bit. LOL Wanda please don't be mad at me!!!!! ARRGH! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From craigak12 <@t> gmail.com Mon Apr 1 17:36:12 2013 From: craigak12 <@t> gmail.com (Jb) Date: Mon Apr 1 17:36:20 2013 Subject: [Histonet] IHC pipettes: Message-ID: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> What is the best pipette to buy for IHC? What ul range is best used? Thank you- Sent from my iPhone From liz <@t> premierlab.com Mon Apr 1 17:42:36 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 1 17:42:37 2013 Subject: [Histonet] IHC pipettes: In-Reply-To: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> References: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC29CF@SBS2K8.premierlab.local> Most will work fine we have used Biohit and Eppendorf, you just need to take care of them, you need to purchase a stand, most vendors will run some deal when you purchase an entire set. I would purchase 3 - 4 different pipettors. We calibrate every 3 months. - 0.5 ul to 10 ul - 2 ul to 20 ul - 20 ul to 200 ul - 100 ul to 1000 ul Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, April 01, 2013 4:36 PM To: histonet Subject: [Histonet] IHC pipettes: What is the best pipette to buy for IHC? What ul range is best used? Thank you- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Mon Apr 1 17:58:09 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Apr 1 17:58:18 2013 Subject: [Histonet] IHC pipettes: In-Reply-To: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> References: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F093D196845@EXCHMB01.isis.local> Eppendorf and the 4 different sizes, that Liz mentioned. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, April 01, 2013 3:36 PM To: histonet Subject: [Histonet] IHC pipettes: What is the best pipette to buy for IHC? What ul range is best used? Thank you- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Apr 1 16:08:31 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 1 18:23:15 2013 Subject: [Histonet] slide printer Message-ID: Surgipath used to have a small slide printer. It would print individual slides. Does anyone know if there is still such a printer available? Thanks, Jennifer Mt. San Antonio College From rjbuesa <@t> yahoo.com Mon Apr 1 19:32:38 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 1 19:32:41 2013 Subject: [Histonet] IHC pipettes: In-Reply-To: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> References: <07551605-19D5-4CF5-9197-0C7CAAAF1746@gmail.com> Message-ID: <1364862758.78382.YahooMailNeo@web163103.mail.bf1.yahoo.com> Eppendorf Ren? J. From: Jb To: histonet Sent: Monday, April 1, 2013 6:36 PM Subject: [Histonet] IHC pipettes: What is the best pipette to buy for IHC?? What ul range is best used? Thank you- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Tue Apr 2 00:32:22 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Apr 2 00:32:34 2013 Subject: [Histonet] minus 80 freezer safe time in power outage In-Reply-To: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> Message-ID: <0C995E1C-42A4-4B26-A3A3-D6E35FB65515@gmail.com> Hello Tim, When I worked at UCSF, we had five -80C sub zero freezers stored in a cool room AND on those occasions, when a freezer did malfunction, our "safe" temperature was -50C - mostly because ALL our frozen specimens were brain. We had a protocol in place for checking each freezer (weekly) for ice blockage/build-up on the door & around the front inside, so as not to create problems w/the freezers...later. However, when a freezer malfunction did occur it was mostly because of the compressor unit (some freezers have 2 compressors.) When a compressor (or any other part) on a particular freezer was replaced, this information was always recorded. In any case, we never opened the door, except to quickly place 1 (5-lb) block of dry ice per shelf, to keep the low temperature in the freezer constant (overnight) until an outside repair technician could come & make repairs the next day. **We always kept a large supply of dry ice. During my 7 years at UCSF, there was only 1 time, we had to transfer all tissue racks into another -80C freezer. We were very lucky, because we had just purchased a new freezer & the freezer was turned ON the night before. Tim, I hope this helps. Let me know if you require any further help. Maria Mejia Affymetrix, Inc. On Mar 29, 2013, at 8:16 AM, Morken, Timothy wrote: > Hi all, For a minus 80 freezer what would you consider a "safe" time period that it can go without power before the temperature gets too high and contents need to be transferred (assuming door stays closed)? I'm thinking -40 would be a cutoff temp to move samples. Anyone else think differently about that? > > Thanks for any info! > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94143 > > (415) 353-1266 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dont8know8me <@t> yahoo.com Tue Apr 2 04:01:22 2013 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Tue Apr 2 04:01:30 2013 Subject: [Histonet] salutations Message-ID: <1364893282.18891.YahooMailNeo@web120105.mail.ne1.yahoo.com> http://www.selvamonos.org/includes/hotnews.php?ygmfdje712eg __________________________ Sociologists years ago discovered that the averaged opinion of a mass of equally expert (or equally ignorant) observers is quite a bit more reliable a predictor than that of a single randomly-chosen one of the observers. They called this the "Delphi effect." It appears that what Linus has shown is that this applies even to debugging an operating system - that the Delphi effect can tame development complexity even at the complexity level of an OS kernel. -- Eric S. Raymond (The Cathedral and the Bazaar) From lblazek <@t> digestivespecialists.com Tue Apr 2 06:45:15 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 2 06:47:14 2013 Subject: [Histonet] slide printer In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39164EEB1B82@IBMB7Exchange.digestivespecialists.com> Jennifer, I have the Primera Signature Slide printer made by Primera Technology and sold by Creative Waste Solutions. http://cwsincorp.com/ I really like it. You can use it as a stand-alone or connected to your APLIS. It's compact and works very well. You can print your slides with a color band across the top to distinguish different stains or types of tissue if you want or add bar coding. I don't use bar coding yet so I can't say how well that function works. The printing on the slides is very good. There hasn't been any smudging or fading. There is a little bit of babysitting but not much. Tech support is fast and reliable when needed. If I can answer any other questions feel free to get in touch with me. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, April 01, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printer Surgipath used to have a small slide printer. It would print individual slides. Does anyone know if there is still such a printer available? Thanks, Jennifer Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Apr 2 07:28:47 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Apr 2 07:28:54 2013 Subject: [Histonet] slide printer In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39164EEB1B82@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39164EEB1B82@IBMB7Exchange.digestivespecialists.com> Message-ID: I may be wrong, but doesn't Sakura sell this same product? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 02, 2013 7:45 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide printer Jennifer, I have the Primera Signature Slide printer made by Primera Technology and sold by Creative Waste Solutions. http://cwsincorp.com/ I really like it. You can use it as a stand-alone or connected to your APLIS. It's compact and works very well. You can print your slides with a color band across the top to distinguish different stains or types of tissue if you want or add bar coding. I don't use bar coding yet so I can't say how well that function works. The printing on the slides is very good. There hasn't been any smudging or fading. There is a little bit of babysitting but not much. Tech support is fast and reliable when needed. If I can answer any other questions feel free to get in touch with me. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, April 01, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printer Surgipath used to have a small slide printer. It would print individual slides. Does anyone know if there is still such a printer available? Thanks, Jennifer Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Apr 2 08:02:23 2013 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Apr 2 08:02:34 2013 Subject: [Histonet] slide printer In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39164EEB1B82@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39164EEB1B82@IBMB7Exchange.digestivespecialists.com> Message-ID: <16F356143B1CE2459BC129BF68AD0F0F03FCA887@PHSX10MB25.partners.org> We recently purchased the Primera slide printer from Creative Waste Sotn's and really like it. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 02, 2013 7:45 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide printer Jennifer, I have the Primera Signature Slide printer made by Primera Technology and sold by Creative Waste Solutions. http://cwsincorp.com/ I really like it. You can use it as a stand-alone or connected to your APLIS. It's compact and works very well. You can print your slides with a color band across the top to distinguish different stains or types of tissue if you want or add bar coding. I don't use bar coding yet so I can't say how well that function works. The printing on the slides is very good. There hasn't been any smudging or fading. There is a little bit of babysitting but not much. Tech support is fast and reliable when needed. If I can answer any other questions feel free to get in touch with me. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, April 01, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printer Surgipath used to have a small slide printer. It would print individual slides. Does anyone know if there is still such a printer available? Thanks, Jennifer Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From GaleL <@t> unionhospital.org Tue Apr 2 08:14:20 2013 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Tue Apr 2 08:14:51 2013 Subject: [Histonet] cassettes Message-ID: Has anyone else had trouble getting white Tissue-Loc cassettes (Histoscreen), or any other color? Ours have been on backorder for almost two months from our supplier and they say it could be at least another month until they are released. We're told it is a manufacturing delay............... Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From Fawn.Bomar <@t> HalifaxRegional.com Tue Apr 2 08:26:37 2013 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Apr 2 08:27:11 2013 Subject: [Histonet] RE: cassettes In-Reply-To: References: Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B94FEE6E@EXCH-2K10.hrhs.com> We haven't been able to get any either. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gale Limron [GaleL@unionhospital.org] Sent: Tuesday, April 02, 2013 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassettes Has anyone else had trouble getting white Tissue-Loc cassettes (Histoscreen), or any other color? Ours have been on backorder for almost two months from our supplier and they say it could be at least another month until they are released. We're told it is a manufacturing delay............... Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From b-frederick <@t> northwestern.edu Tue Apr 2 09:52:12 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Apr 2 09:52:18 2013 Subject: [Histonet] FW: Primera Printer In-Reply-To: <960C05981FB9C6459C161BA31C27229C1AAAA4A2@CHCSPMBX1.ads.northwestern.edu> References: <960C05981FB9C6459C161BA31C27229C1AAAA4A2@CHCSPMBX1.ads.northwestern.edu> Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D15AAB@evcspmbx2.ads.northwestern.edu> >From my manager Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From: Herbert Skip Brown Sent: Tuesday, April 02, 2013 8:27 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Primera Printer Linda/Jennifer: We have just finished doing a thorough evaluation of the Primera Printer. I agree with Linda that it is a smart new system that prints very well in black & white and/or color. We do large clinical trial projects that often require 100+ slide sectioning. The Primera Printer allows you to very easily go in and edit single slides out of a run in case data changes for one slide or there was a typo error by the user. Also and more importantly, the color feature is a tremendous asset to our Q.A. by being able to color code priority cases, individual hospitals or departments, and/or individual microtomists. From the use of color we can now reduce our slide cost by not having to buy various colored slides, and only buying the Epic white slides and print in color. The training was very hands-on via interactive on-line training through their technical support. We are just initiating the unit into our system. Herbert Skip Brown, M.Div., HT(ASCP) Lab Manager - Pathology Core Facility Robert H. Lurie Comprehensive Cancer Center Northwestern University 710 N. Fairbanks Olson 8424 Chicago, Il 60611 (312) 503-3976 From algranth <@t> email.arizona.edu Tue Apr 2 10:09:04 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Apr 2 10:09:13 2013 Subject: [Histonet] cassettes In-Reply-To: References: Message-ID: I just got some from Stat Lab. Got the red ones because we use white labels on the cassettes. I think some colors were difficult to get. Andi From vtobias <@t> uw.edu Tue Apr 2 10:11:55 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Tue Apr 2 10:12:06 2013 Subject: [Histonet] slide printer In-Reply-To: <16F356143B1CE2459BC129BF68AD0F0F03FCA887@PHSX10MB25.partners.org> References: <5A2BD13465E061429D6455C8D6B40E39164EEB1B82@IBMB7Exchange.digestivespecialists.com> <16F356143B1CE2459BC129BF68AD0F0F03FCA887@PHSX10MB25.partners.org> Message-ID: Jennifer, The Sakura model is the same unit. We had a demo of it and really liked it. We tried different slides and the ones with clipped corners worked best. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, April 02, 2013 6:02 AM To: 'Blazek, Linda'; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide printer We recently purchased the Primera slide printer from Creative Waste Sotn's and really like it. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 02, 2013 7:45 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide printer Jennifer, I have the Primera Signature Slide printer made by Primera Technology and sold by Creative Waste Solutions. http://cwsincorp.com/ I really like it. You can use it as a stand-alone or connected to your APLIS. It's compact and works very well. You can print your slides with a color band across the top to distinguish different stains or types of tissue if you want or add bar coding. I don't use bar coding yet so I can't say how well that function works. The printing on the slides is very good. There hasn't been any smudging or fading. There is a little bit of babysitting but not much. Tech support is fast and reliable when needed. If I can answer any other questions feel free to get in touch with me. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, April 01, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printer Surgipath used to have a small slide printer. It would print individual slides. Does anyone know if there is still such a printer available? Thanks, Jennifer Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aakrasht <@t> yahoo.com Tue Apr 2 10:59:24 2013 From: aakrasht <@t> yahoo.com (Ali A Krasht) Date: Tue Apr 2 10:59:32 2013 Subject: [Histonet] ## NEED HELP NEED JOB ## Message-ID: <1364918364.47406.YahooMailNeo@web124704.mail.ne1.yahoo.com> Hi Guys I am a LIS/ Histologist (HT & HTL ASCP Approved) and currently located in Frisco Texas. I graduated with a B.S. in Biology with a minor in Psychology and two yearsMedical Technology including Information Systems Technology and a part time Internet Marketing.? I have ten years of Histology experience/ supervisor including grossing, IHC, IF.?I have worked in both large and small laboratories, in pharmaceuticals testing, reference and clinical environments.? I was laid-off?recently?and the company I was working for closed down. I am seeking a position as a Histologist or/ PA or/?LIS showcasing my extensive experience in Histotechnology, Immunochemistry, Microbiology, Biotechnology, Medical Technology, and Information Technology. I am seeking an organization with a team mentality and opportunities to use my skills to a greater degree and would love an opportunity for growth and development. Please help me if you know anyone out there that can give me a push.? I thought I could find one very?easily?but unfortunately its not happening. Location of?interest: Dallas, Frisco, McKinney, Allen. But I am opened for other locations in the US as a contract or travelling tech. Thank you for your?attention and help. ? Ali A. Krasht 214 444 8319 http://about.me/alikrasht www.linkedin.com/in/alikrasht/ www.linkedin.com/in/alikrasht/ ************************************************ The information transmitted is intended only for the person or entity to which it is addressed and may contain proprietary, business-confidential and/or privileged material. If you are not the intended recipient of this message you are hereby notified that any use, review, retransmission, dissemination, distribution, reproduction or any action taken in reliance upon this message is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Any views expressed in this message are those of the individual sender and may not necessarily reflect the views of the company. ************************************************ From maria.navas <@t> utah.edu Tue Apr 2 12:58:02 2013 From: maria.navas <@t> utah.edu (Maria Navas-Moreno) Date: Tue Apr 2 12:58:55 2013 Subject: [Histonet] Sectioning in microtome - last bit of tissue detaches Message-ID: Hello All, We section fixed frozen brain that we mount on the microtome using either 30% sucrose or OCT and we keep the temperature down by surrounding the tissue with crushed dry ice constantly (every 10-20 sections). The problem I have had, and also the person who trained me also had, is that when getting towards the end of the block a big chunk of the tissue gets lose and ruins the sectioning of that last part. I am fairly new to the sectioning art and I was wondering if anybody has advice to avoid this problem or at least make it a bit better. Thanks, Maria From sdysart <@t> mirnarx.com Tue Apr 2 16:36:00 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Apr 2 16:36:59 2013 Subject: [Histonet] Out of my comfort zone... Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> So...I have been asked to do some micro-dissection on some slides and then do downstream RT/PCR on them. My molecular knowledge doesn't go much out of the world of IHC so...here is my question... Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution you are using) for proteinase K for use in RNA isolation and then later PCR? Does this work? The main question is will the HIER step take off the formalin linkage from the nucleic acids, or just the protein? One last thing is what else goes into these solutions other than Citrate Buffer and Tween? I haven't made it up in forever, I have just been ordering it from companies...I know...lazy... Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From ajohnston <@t> oregonmed.net Tue Apr 2 16:44:15 2013 From: ajohnston <@t> oregonmed.net (Johnston, Amy) Date: Tue Apr 2 16:44:24 2013 Subject: [Histonet] Methanol In-Reply-To: <51538E2A.4090100@comcast.net> References: <8F6C0286892B2544B10E5F31F1C7075D27E1363E@IPSPRDMBOX1.oregonmed.net> <51538E2A.4090100@comcast.net> Message-ID: <8F6C0286892B2544B10E5F31F1C7075D27E13B5D@IPSPRDMBOX1.oregonmed.net> Thank you, But they weren't concerned with the methanol in the formalin, it was the methanol that the med techs were using. My question is really how do you know when you have to do air quality testing. Is it a question of quantity of a chemical? Amy From: darkdaym@comcast.net [mailto:darkdaym@comcast.net] Sent: Wednesday, March 27, 2013 5:26 PM To: Johnston, Amy Subject: Re: [Histonet] Methanol Amy, Are you aware that Formalin Fixative contains Methanol, usually a little over 1%? The label and MSDS should state this. Mark On 3/27/2013 6:47 PM, Johnston, Amy wrote: Hello all, I work in a medical lab that has recently expanded to histology and we are working on our chemical hygiene plan. We have already done the initial air sample testing for formaldehyde and xylene, but we were told by someone at OSHA that we need to test for methanol as well. The only place that methanol is used is on one of the med tech's machines and it is only 4 ml occasionally. I'm not sure if this is something we need to include in our plan, it was never tested for in the past. How can we find out specifically which chemicals we need to monitor? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Apr 2 17:12:10 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 2 17:12:22 2013 Subject: [Histonet] FW: Primera Printer In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF20D15AAB@evcspmbx2.ads.northwestern.edu> References: <960C05981FB9C6459C161BA31C27229C1AAAA4A2@CHCSPMBX1.ads.northwestern.edu> <62C639732D3F274DACED033EBDF6ADAF20D15AAB@evcspmbx2.ads.northwestern.edu> Message-ID: <515B1F7A.7770.0077.1@harthosp.org> Can this printer be interfaced with CoPath? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Bernice Frederick 4/2/2013 10:52 AM >>> >From my manager Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From: Herbert Skip Brown Sent: Tuesday, April 02, 2013 8:27 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Primera Printer Linda/Jennifer: We have just finished doing a thorough evaluation of the Primera Printer. I agree with Linda that it is a smart new system that prints very well in black & white and/or color. We do large clinical trial projects that often require 100+ slide sectioning. The Primera Printer allows you to very easily go in and edit single slides out of a run in case data changes for one slide or there was a typo error by the user. Also and more importantly, the color feature is a tremendous asset to our Q.A. by being able to color code priority cases, individual hospitals or departments, and/or individual microtomists. From the use of color we can now reduce our slide cost by not having to buy various colored slides, and only buying the Epic white slides and print in color. The training was very hands-on via interactive on-line training through their technical support. We are just initiating the unit into our system. Herbert Skip Brown, M.Div., HT(ASCP) Lab Manager - Pathology Core Facility Robert H. Lurie Comprehensive Cancer Center Northwestern University 710 N. Fairbanks Olson 8424 Chicago, Il 60611 (312) 503-3976 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Apr 2 17:21:55 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Apr 2 17:22:04 2013 Subject: [Histonet] FW: Primera Printer In-Reply-To: <515B1F7A.7770.0077.1@harthosp.org> References: <960C05981FB9C6459C161BA31C27229C1AAAA4A2@CHCSPMBX1.ads.northwestern.edu> <62C639732D3F274DACED033EBDF6ADAF20D15AAB@evcspmbx2.ads.northwestern.edu> <515B1F7A.7770.0077.1@harthosp.org> Message-ID: And the answer is.... YES!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, April 02, 2013 6:12 PM To: histonet@lists.utsouthwestern.edu; Bernice Frederick Subject: Re: [Histonet] FW: Primera Printer Can this printer be interfaced with CoPath? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Bernice Frederick 4/2/2013 10:52 AM >>> >>> >From my manager Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From: Herbert Skip Brown Sent: Tuesday, April 02, 2013 8:27 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Primera Printer Linda/Jennifer: We have just finished doing a thorough evaluation of the Primera Printer. I agree with Linda that it is a smart new system that prints very well in black & white and/or color. We do large clinical trial projects that often require 100+ slide sectioning. The Primera Printer allows you to very easily go in and edit single slides out of a run in case data changes for one slide or there was a typo error by the user. Also and more importantly, the color feature is a tremendous asset to our Q.A. by being able to color code priority cases, individual hospitals or departments, and/or individual microtomists. From the use of color we can now reduce our slide cost by not having to buy various colored slides, and only buying the Epic white slides and print in color. The training was very hands-on via interactive on-line training through their technical support. We are just initiating the unit into our system. Herbert Skip Brown, M.Div., HT(ASCP) Lab Manager - Pathology Core Facility Robert H. Lurie Comprehensive Cancer Center Northwestern University 710 N. Fairbanks Olson 8424 Chicago, Il 60611 (312) 503-3976 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From JMacDonald <@t> mtsac.edu Tue Apr 2 17:49:35 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 2 17:49:44 2013 Subject: [Histonet] Methanol In-Reply-To: <8F6C0286892B2544B10E5F31F1C7075D27E13B5D@IPSPRDMBOX1.oregonmed.net> References: <8F6C0286892B2544B10E5F31F1C7075D27E1363E@IPSPRDMBOX1.oregonmed.net> <51538E2A.4090100@comcast.net> <8F6C0286892B2544B10E5F31F1C7075D27E13B5D@IPSPRDMBOX1.oregonmed.net> Message-ID: We had methanol monitored at one facility that I worked at. If you have it done once and it is below the PEL and you don't make changes to the procedure/use you don't need to do it again. From: "Johnston, Amy" To: "darkdaym@comcast.net" Cc: "histonet@lists.utsouthwestern.edu" Date: 04/02/2013 02:45 PM Subject: RE: [Histonet] Methanol Sent by: histonet-bounces@lists.utsouthwestern.edu Thank you, But they weren't concerned with the methanol in the formalin, it was the methanol that the med techs were using. My question is really how do you know when you have to do air quality testing. Is it a question of quantity of a chemical? Amy From: darkdaym@comcast.net [mailto:darkdaym@comcast.net] Sent: Wednesday, March 27, 2013 5:26 PM To: Johnston, Amy Subject: Re: [Histonet] Methanol Amy, Are you aware that Formalin Fixative contains Methanol, usually a little over 1%? The label and MSDS should state this. Mark On 3/27/2013 6:47 PM, Johnston, Amy wrote: Hello all, I work in a medical lab that has recently expanded to histology and we are working on our chemical hygiene plan. We have already done the initial air sample testing for formaldehyde and xylene, but we were told by someone at OSHA that we need to test for methanol as well. The only place that methanol is used is on one of the med tech's machines and it is only 4 ml occasionally. I'm not sure if this is something we need to include in our plan, it was never tested for in the past. How can we find out specifically which chemicals we need to monitor? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kjgada <@t> gmail.com Tue Apr 2 19:58:01 2013 From: kjgada <@t> gmail.com (Komal Gada) Date: Tue Apr 2 19:58:05 2013 Subject: [Histonet] Tampa Area Internship Partner Needed Message-ID: Hello Histo-netters! Just wanted to reach out once again specifically for the Tampa, Florida area. We are in need of an internship partner beginning May 6th, for two months. Please contact me directly if you are interested. As always, thank you for any assistance, Komal Keiser University From SHUNTER <@t> beaumont.edu Wed Apr 3 07:08:26 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Apr 3 07:08:32 2013 Subject: [Histonet] RE: Out of my comfort zone... In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: Sarah The proteinase K does a lot more than break the formalin linkages. To isolate the RNA or DNA you have to break up the cell membranes, nuclear membranes and all the other proteins in the cellular matrix to isolate the nucleic acids. I don't think an antigen retrieval solution will do any of that. We use a kit from Qiagen that is very easy to use. Also, if you haven't done much RNA work, remember that there are RNAses everwhere. Wear gloves, wipe down your microtome with RNAse away or some other such product. Use a clean blade. Discard the first couple of cuts from your block. Too many fingers have touched them. We usually cut 10um sections for our extractions. Use clean water in your waterbath - fresh just for your RNA tissues. We keep slide boxes separate for RNA work so bare fingers don't touch them. As for how many sections - it depends on how much message you will be looking for. You will have to try your method to find out. If you cut curls for extractions, we use 10um curls. Use disposable plastic tubes as these are mostly RNAse free. We routinely isolate sufficient quantities of good RNA from FFPE tissues, but you still need to use good RNA technique. If you are making up your own master mixes and primer mixes, be sure to use RNAse free water. Good luck Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Tuesday, April 02, 2013 5:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Out of my comfort zone... So...I have been asked to do some micro-dissection on some slides and then do downstream RT/PCR on them. My molecular knowledge doesn't go much out of the world of IHC so...here is my question... Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution you are using) for proteinase K for use in RNA isolation and then later PCR? Does this work? The main question is will the HIER step take off the formalin linkage from the nucleic acids, or just the protein? One last thing is what else goes into these solutions other than Citrate Buffer and Tween? I haven't made it up in forever, I have just been ordering it from companies...I know...lazy... Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Wed Apr 3 09:55:28 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Apr 3 09:55:39 2013 Subject: [Histonet] RE: Out of my comfort zone... In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: Hello Just wanted to add one more thing - we actually use a dedicated pyrex dish (maybe 6x10 inches) for our water bath for RNA sections. We use warm tap water, but you can put it in the microwave for a short bit if it needs to be warmer. You can spray the dish with RNAse away and wipe before filling with water. Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Tuesday, April 02, 2013 5:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Out of my comfort zone... So...I have been asked to do some micro-dissection on some slides and then do downstream RT/PCR on them. My molecular knowledge doesn't go much out of the world of IHC so...here is my question... Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution you are using) for proteinase K for use in RNA isolation and then later PCR? Does this work? The main question is will the HIER step take off the formalin linkage from the nucleic acids, or just the protein? One last thing is what else goes into these solutions other than Citrate Buffer and Tween? I haven't made it up in forever, I have just been ordering it from companies...I know...lazy... Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Wed Apr 3 11:11:14 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Apr 3 11:11:24 2013 Subject: [Histonet] RE: Out of my comfort zone... In-Reply-To: References: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: We have been using store bought gallons of distilled water in our water baths. This water has been boiled so enzyme activity should be absent. Helen 410.614.1660 http://tmalab.jhmi.edu/ http://prostatebiorepository.org/ Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Wednesday, April 03, 2013 10:55 AM To: Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Out of my comfort zone... Hello Just wanted to add one more thing - we actually use a dedicated pyrex dish (maybe 6x10 inches) for our water bath for RNA sections. We use warm tap water, but you can put it in the microwave for a short bit if it needs to be warmer. You can spray the dish with RNAse away and wipe before filling with water. Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Tuesday, April 02, 2013 5:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Out of my comfort zone... So...I have been asked to do some micro-dissection on some slides and then do downstream RT/PCR on them. My molecular knowledge doesn't go much out of the world of IHC so...here is my question... Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution you are using) for proteinase K for use in RNA isolation and then later PCR? Does this work? The main question is will the HIER step take off the formalin linkage from the nucleic acids, or just the protein? One last thing is what else goes into these solutions other than Citrate Buffer and Tween? I haven't made it up in forever, I have just been ordering it from companies...I know...lazy... Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Apr 3 11:32:42 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 3 11:32:46 2013 Subject: [Histonet] RE: Out of my comfort zone... In-Reply-To: References: <8A70A9B2ECDD084DACFE6C59FCF86D5055F19251@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: We use a heat block during digestion. There is less chance of contamination with a heat block than with a water bath. ... and no we don't use antigen retrieval solution for this! We use a Qiagen kit too. Mark On Wed, Apr 3, 2013 at 9:11 AM, Helen Fedor wrote: > We have been using store bought gallons of distilled water in our water > baths. This water has been boiled so enzyme activity should be absent. > > > Helen > > 410.614.1660 > > http://tmalab.jhmi.edu/ > http://prostatebiorepository.org/ > > > > Helen > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter > Sent: Wednesday, April 03, 2013 10:55 AM > To: Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Out of my comfort zone... > > Hello > Just wanted to add one more thing - we actually use a dedicated pyrex dish > (maybe 6x10 inches) for our water bath for RNA sections. We use warm tap > water, but you can put it in the microwave for a short bit if it needs to > be warmer. You can spray the dish with RNAse away and wipe before filling > with water. > Sue > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart > Sent: Tuesday, April 02, 2013 5:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Out of my comfort zone... > > So...I have been asked to do some micro-dissection on some slides and then > do downstream RT/PCR on them. My molecular knowledge doesn't go much out > of the world of IHC so...here is my question... > > Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution > you are using) for proteinase K for use in RNA isolation and then later > PCR? Does this work? The main question is will the HIER step take off the > formalin linkage from the nucleic acids, or just the protein? > > One last thing is what else goes into these solutions other than Citrate > Buffer and Tween? I haven't made it up in forever, I have just been > ordering it from companies...I know...lazy... > > Thanks > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From galinadeyneko <@t> yahoo.com Wed Apr 3 11:42:15 2013 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Wed Apr 3 11:42:20 2013 Subject: [Histonet] Re: cell pellet preparation Message-ID: <1365007335.15205.YahooMailClassic@web160201.mail.bf1.yahoo.com> I attach my protocol for cell pellet ? Preparation of formalin fixed Cell pellets. 1. Grow up approximately 5-1o millions cells. 2. Centrifuge?the cells with the medium in conical tubes for 10 minutes with speed 1000rpm. 3. Remove supernatant, wash once with 1X PBS at RT, centrifuge as before and discard PBS supernatant. 4. Place 2 ml or more of 10 % formalin in tube, flick to break up pellet, and re-suspend the cells in formalin and fixed 20 minutes at RT. 5. Centrifuge as before and remove formalin supernatant. 6. Wash once with 1X PBS, at RT, centrifuge as before and discard PBS supernatant. 7. Add 1 ml of 1X PBS, carefully re-suspend the cells and transfer to 1.5 ml Eppendorf tube. 8. Microfuge 10 minutes at 0.8 xg (2900 rpm) and remove as much PBS supernatant as possible. 9. Warm the Histogel (Allan Scientific # HG4000-012) in microwave oven 10-20 seconds till Histogel turns to liquid. Let it cool a bit. 10. Re-suspend cells in 100-150 ?l of warm Histogel, leave pellet undisturbed for at least 20 minutes in wet ice for gel solidifying. 11. Remove the solid gel pyramid with sharp wood stick, wrap in histo paper place in cassette and process? ? ? Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w ? From galinadeyneko <@t> yahoo.com Wed Apr 3 11:50:25 2013 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Wed Apr 3 11:50:29 2013 Subject: [Histonet] Re:Rabbit antibody on rabbit tissues Message-ID: <1365007825.51838.YahooMailClassic@web160203.mail.bf1.yahoo.com> Dear Colleagues ?My current problem is: I should detect the myeloperoxidase? (MPO) antigen and its derivate nitrotyrosine and chlorotyrosine in the rabbit tissues (aorta) . Most of the antibodies what I found and claimed to work on Rabbit are rabbit polyclonal. Do you?know ?antibodies for those antigens from any other species or biotinylated antibodies, or you can suggest good secondary antibodies and detection systems that might work on rabbit tissues with rabbit primary, on the analogue of mouse-on-mouse detection system. I already tested goat and mouse anti-human MPO on rabbit aorta and lung/liver as a positive control, but? besides stained macrophages and endothelial cells, i got stained smooth muscles in the media, and I am not sure that the staining in macrophages is specific. Maybe yes, since negative controls with mouse and goat IgG are absolutely clean. Can you share you advices, opinion, experience. ? Thank you in advance Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w --- On From brett_connolly <@t> merck.com Wed Apr 3 12:26:55 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Apr 3 12:27:01 2013 Subject: [Histonet] Processing question Message-ID: Histonetters, Has anyone tried to process an intact mouse brain to paraffin? Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Stephen.Clark1 <@t> hcahealthcare.com Wed Apr 3 12:44:50 2013 From: Stephen.Clark1 <@t> hcahealthcare.com (Stephen.Clark1@hcahealthcare.com) Date: Wed Apr 3 12:45:00 2013 Subject: [Histonet] Ventana Melanoma Cocktail Message-ID: <213A638666ECDB4A8A81B5CF8646CC0B74DD6BB4F1@NADCWPMSGCMS05.hca.corpad.net> I've got a question regarding Ventana's Melanoma Cocktail. We've never done double or triple staining at our institution so my knowledge of the CPT codes for this is vague. If we were to use this product, which has HMB-45, Tyrosinase, and Mart-1 in one dispenser, do I bill it as an 88342 three times? Or is there a separate CPT code that we would use? Thanks for your input. Steve Clark Pathology Dept. Supervisor Grand Strand Regional Medical Center 843-692-1486 Lab 843-692-1459 Desk Stephen.Clark1@hcahealthcare.com [cid:image001.gif@01C9DADA.65BB9E10] From JEllin <@t> yumaregional.org Wed Apr 3 12:53:10 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Apr 3 12:53:19 2013 Subject: [Histonet] RE: Ventana Melanoma Cocktail In-Reply-To: <213A638666ECDB4A8A81B5CF8646CC0B74DD6BB4F1@NADCWPMSGCMS05.hca.corpad.net> References: <213A638666ECDB4A8A81B5CF8646CC0B74DD6BB4F1@NADCWPMSGCMS05.hca.corpad.net> Message-ID: You can only bill for 1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen.Clark1@hcahealthcare.com Sent: Wednesday, April 03, 2013 10:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Melanoma Cocktail I've got a question regarding Ventana's Melanoma Cocktail. We've never done double or triple staining at our institution so my knowledge of the CPT codes for this is vague. If we were to use this product, which has HMB-45, Tyrosinase, and Mart-1 in one dispenser, do I bill it as an 88342 three times? Or is there a separate CPT code that we would use? Thanks for your input. Steve Clark Pathology Dept. Supervisor Grand Strand Regional Medical Center 843-692-1486 Lab 843-692-1459 Desk Stephen.Clark1@hcahealthcare.com [cid:image001.gif@01C9DADA.65BB9E10] ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Ronald.Houston <@t> nationwidechildrens.org Wed Apr 3 15:23:27 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Apr 3 15:23:36 2013 Subject: [Histonet] Senior Histoetech vacancy Message-ID: We have a vacancy for a Senior Histotechnian in our progressive department. Experience in IHC a must. IF, ISH and /or EM desirable. Must be willing to participate in and lead part of formal Continuing Education program for all staff. Experience in QA development and Lean/Six Sigma projects a plus. Please contact me for more details or apply on-line at http://www.nationwidechildrens.org/healthcare-career-opportunities Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From carmen_loiselle <@t> hotmail.com Wed Apr 3 15:44:50 2013 From: carmen_loiselle <@t> hotmail.com (carmen loiselle) Date: Wed Apr 3 15:44:53 2013 Subject: [Histonet] Nail treatment and bone marrow decalcification info needed Message-ID: Hello fellow histonetters , I need your expertise regarding the treatment of nails. We currently immerse the nails specimen in a solution of tween & formalin up to 2 weeks . We then process and section them. Is there a more rapid and efficient way to do it ??? Also, we recently change our procedure for the fixation of the bone marrow specimen. Up to last week we were using Zenker's solution . Starting in april we are now using the formalin fixation for 24 hours before decalcification. What are the most efficient and rapid way of doing the decalcification without altering the IHC markers currently use for these type of specimen ??? At the present time we are using 10% formic acid for 2 hours, rinse then process. What about RDO agent (which I believe is HCL based) which is faster ???? Thank you in advance for all your responses Carmen From rjbuesa <@t> yahoo.com Wed Apr 3 15:59:20 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 3 15:59:29 2013 Subject: [Histonet] Nail treatment and bone marrow decalcification info needed In-Reply-To: References: Message-ID: <1365022760.16647.YahooMailNeo@web163102.mail.bf1.yahoo.com> Use 10% aq. sol. of sodium or potassium hydroxide for 30 minutes on already fixed toe (or finger) nails, wash thoroughly, and process as usual. Decalcify BM with EDTA overnight Ren? J. From: carmen loiselle To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, April 3, 2013 4:44 PM Subject: [Histonet] Nail treatment and bone marrow decalcification info needed Hello fellow histonetters , I need your expertise regarding the treatment of nails.? We currently immerse the nails specimen in a solution of tween & formalin up to 2 weeks .? We then process and section them.? Is there a more rapid and efficient way to do it ??? Also, we recently change our procedure for the fixation of the bone marrow specimen.? Up to last week we were using Zenker's solution .? Starting in april we are now using the formalin fixation for 24 hours before decalcification.? What are the most efficient and rapid way of doing the decalcification without altering the IHC markers? currently use for these type of specimen ??? At the present time we are using 10% formic acid for 2 hours, rinse then process.? What about RDO agent? (which I believe is HCL based)? which is faster ???? Thank you in advance for all your responses Carmen ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maria.navas <@t> utah.edu Wed Apr 3 20:06:24 2013 From: maria.navas <@t> utah.edu (Maria De Los Angeles Navas) Date: Wed Apr 3 20:06:03 2013 Subject: [Histonet] Sectioning in sliding microtome issues Message-ID: Hello All, We section fixed frozen brain that we mount on the microtome using either 30% sucrose or OCT and we keep the temperature down by surrounding the tissue with crushed dry ice constantly (every 10 minutes). One of the problems I have had, and also the person who trained me also had, is that when getting towards the end of the block a big chunk of the tissue gets lose and ruins the sectioning of that last part. Any tricks, tips to make this better? Also, this week the microtome has been skipping one section all together and cutting the next one twice as thick. We have a Thermo HM 450 microtome and I was wondering if people have experienced this problem with this same microtome (or others) and if you know what the problem could be and how to go around it. I don't think is temperature, while testing the microtome today I was being extra careful by keeping the temp constant, but you never know for sure. Thanks sooo much, Maria From mbmphoto <@t> gmail.com Wed Apr 3 23:07:42 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Wed Apr 3 23:07:51 2013 Subject: [Histonet] Sectioning in sliding microtome issues In-Reply-To: References: Message-ID: <7EB429A9-5D21-4E6A-998C-7EAE20ECC767@gmail.com> Hello Maria, Well, it's interesting that the Thermo HM 450 sliding microtome, looks very much like the Microm HM450 sliding microtome that I used (A LOT) at UCSF. You must be cutting (30um or 40um??) free-floating sections. Questions: > How thick (mm) are the frozen brain block? > AND, are you cutting a whole brain from a non-human primate? > Are you using a solid brass freezing stage - mounted to your sliding microtome? The type with 2 small wells (1 in the front & 1 in the back). If you're cutting a (whole) brain block - large or small, I would strongly recommend the use of Cryogel - which is the bomb! I've used this stuff to cut excellent sections from 1mm to 9mm thick frozen brain blocks. Switching to cryogel, will significantly reduce your rate of loosing chunks of tissue at the end of the block because it supports like no other freezing resin. When you use cryogel or OCT or any other freezing media, make sure there are NO air bubbles in the supporting media. I would also check all the knobs on your sliding microtome & freezing stage - make sure that nothing is loose! Equally important, is maintaining consistent freezing temperature of the frozen brain block & of the supporting resin during sectioning. Especially since the sliding microtome is not in a cold chamber like a cryostat. So, if your cutting room is too warm - this warm environment will dramatically affect your freezing temperature of tissue block & resin AND ultimately your cutting. For this reason, I chose to use the Physitemp freezing stage - the freezing stages come in 3 sizes and at UCSF we used the larger one - the BFS-30MP (4x4 inches square). Here is the link; http://www.physitemp.com/products/FreezingMicroTomes/bfs-mp.html You'll need a water tank - link: http://www.physitemp.com/products/accessories/ptu3.html If you would like my freezing protocol, just let me know. Regards Maria Mejia Affymetrix, Inc On Apr 3, 2013, at 6:06 PM, Maria De Los Angeles Navas wrote: > Hello All, > > We section fixed frozen brain that we mount on the microtome using either 30% sucrose or OCT and we keep the temperature down by surrounding the tissue with crushed dry ice constantly (every 10 minutes). > > One of the problems I have had, and also the person who trained me also had, is that when getting towards the end of the block a big chunk of the tissue gets lose and ruins the sectioning of that last part. Any tricks, tips to make this better? > > Also, this week the microtome has been skipping one section all together and cutting the next one twice as thick. We have a Thermo HM 450 microtome and I was wondering if people have experienced this problem with this same microtome (or others) and if you know what the problem could be and how to go around it. I don't think is temperature, while testing the microtome today I was being extra careful by keeping the temp constant, but you never know for sure. > > > Thanks sooo much, > > Maria > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 4 08:29:48 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 4 08:29:54 2013 Subject: [Histonet] Sectioning in sliding microtome issues In-Reply-To: References: Message-ID: <1365082188.1000.YahooMailNeo@web163103.mail.bf1.yahoo.com> I used a Leica horizontal microtome. One of the ways of eliminating (pushing away) the piece of tissue at the end of the block is to?reduce the?angle of the sliding path of the steel blade and instead of placing it to move at 90?C to the length of the microtome to about half (50?C), reducing the "pushing" effect of the blade?on the end border of the block. Ren? J. From: Maria De Los Angeles Navas To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, April 3, 2013 9:06 PM Subject: [Histonet] Sectioning in sliding microtome issues Hello All, We section fixed frozen brain that we mount on the microtome using either 30% sucrose or OCT and we keep the temperature down by surrounding the tissue with crushed dry ice constantly (every 10 minutes). One of the problems I have had, and also the person who trained me also had, is that when getting towards the end of the block a big chunk of the tissue gets lose and ruins the sectioning of that last part. Any tricks, tips to make this better? Also, this week the microtome has been skipping one section all together and cutting the next one twice as thick. We have a Thermo HM 450 microtome and I was wondering if people have experienced this problem with this same microtome (or others) and if you know what the problem could be and how to go around it. I don't think is temperature, while testing the microtome today I was being extra careful by keeping the temp constant, but you never know for sure. Thanks sooo much, Maria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sarahholt_ht <@t> yahoo.com Thu Apr 4 09:50:58 2013 From: sarahholt_ht <@t> yahoo.com (Sarah Holt) Date: Thu Apr 4 09:51:03 2013 Subject: [Histonet] how are you? Message-ID: <1365087058.69451.YahooMailNeo@web142301.mail.bf1.yahoo.com> http://www.love2china.com/includes/hello.php?jfzcopje712eufuiu ======================= Did you know ... That no-one ever reads these things? From sarahholt_ht <@t> yahoo.com Thu Apr 4 09:52:37 2013 From: sarahholt_ht <@t> yahoo.com (Sarah Holt) Date: Thu Apr 4 09:52:45 2013 Subject: [Histonet] how are you? In-Reply-To: <1365087058.69451.YahooMailNeo@web142301.mail.bf1.yahoo.com> References: <1365087058.69451.YahooMailNeo@web142301.mail.bf1.yahoo.com> Message-ID: <33DD8072-C4BB-485E-93A1-2ACE78866704@yahoo.com> Looks like spam. Sorry about that! Sarah Sent from my iPhone On Apr 4, 2013, at 10:50 AM, Sarah Holt wrote: > http://www.love2china.com/includes/hello.php?jfzcopje712eufuiu > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > ======================= > Did you know ... That no-one ever reads these things? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Apr 4 11:32:52 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Apr 4 11:32:57 2013 Subject: [Histonet] CEU's Message-ID: <1365093172.14273.YahooMailNeo@web140605.mail.bf1.yahoo.com> Hi All: ? I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry.? I am a dinosaur who?received her HT in 1968 and my HTL in 1982, so do not need to renew mine.? ? Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com From wbenton <@t> cua.md Thu Apr 4 11:51:24 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Apr 4 11:53:38 2013 Subject: [Histonet] CEU's In-Reply-To: <1365093172.14273.YahooMailNeo@web140605.mail.bf1.yahoo.com> References: <1365093172.14273.YahooMailNeo@web140605.mail.bf1.yahoo.com> Message-ID: <0B8979A204680A42B93A52B486088CD934229103DC@CUAEXH1.GCU-MD.local> AT, BB, C, CG, CT, H, HT, HTL, M, MB Certified in 2004 or later require 36 points with ? 1 point in laboratory or patient safety (i.e., quality control, quality assurance) ? 2 points in area in which you are certified ? Remaining points in area of specialty, management, education, or other related laboratory areas of interest http://ascp.org/PDF/BOC-PDFs/CMP/CMPBooklet.aspx Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison [akemiat3377@yahoo.com] Sent: Thursday, April 04, 2013 12:32 PM To: Histonet Subject: [Histonet] CEU's Hi All: I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry. I am a dinosaur who received her HT in 1968 and my HTL in 1982, so do not need to renew mine. Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 Email: aallison@montereygi.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From wdesalvo.cac <@t> outlook.com Thu Apr 4 11:53:42 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Apr 4 11:53:46 2013 Subject: [Histonet] CEU's In-Reply-To: <1365093172.14273.YahooMailNeo@web140605.mail.bf1.yahoo.com> References: <1365093172.14273.YahooMailNeo@web140605.mail.bf1.yahoo.com> Message-ID: 36 hours in three years. Go to: http://www.ascp.org/Board-of-Certification William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Thu, 4 Apr 2013 09:32:52 -0700 > From: akemiat3377@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CEU's > > Hi All: > > I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry. I am a dinosaur who received her HT in 1968 and my HTL in 1982, so do not need to renew mine. > > Akemi Allison-Tacha, BS, HT (ASCP) HTL > Pathology Manager > Monterey Bay GI Consultants > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > (381) 375-3577 X117 > Email: aallison@montereygi.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Apr 4 12:13:27 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Apr 4 12:13:37 2013 Subject: [Histonet] CEU's In-Reply-To: References: <1365093172.14273.YahooMailNeo@web140605.mail.bf1.yahoo.com> Message-ID: <1365095607.90211.YahooMailNeo@web140601.mail.bf1.yahoo.com> Thanks everybody! Jim you are one of the most sweetest dinosaur's that has survived and glad you are my friend! ? Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com ? ? ________________________________ From: WILLIAM DESALVO To: Akemi Allison ; histonet Sent: Thursday, April 4, 2013 9:53 AM Subject: RE: [Histonet] CEU's 36 hours in three years. ? Go to:? http://www.ascp.org/Board-of-Certification William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality?Management Committee Owner/Consultant, Collaborative Advantage Consulting ? > Date: Thu, 4 Apr 2013 09:32:52 -0700 > From: akemiat3377@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CEU's > > Hi All: > ? > I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry.? I am a dinosaur who?received her HT in 1968 and my HTL in 1982, so do not need to renew mine.? > ? > Akemi Allison-Tacha, BS, HT (ASCP) HTL > Pathology Manager > Monterey Bay GI Consultants > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > (381) 375-3577? X117 > Email: aallison@montereygi.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBUSTAMANTE <@t> cvm.tamu.edu Thu Apr 4 12:19:03 2013 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Thu Apr 4 12:19:09 2013 Subject: [Histonet] Workshop in Texas Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959ED68@CVMMB02.cvm.tamu.edu> Does anyone knows when this workshop will be offered again?. Maybe on line? Workshop: Are you ready for the HT/HTL exam? Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From BZIMMERM <@t> gru.edu Thu Apr 4 13:10:49 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Apr 4 13:10:59 2013 Subject: [Histonet] Do you know Jack?... Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF761D11@EX-MLB-03.ad.georgiahealth.edu> Or Wanda and Lamar? Bill? Vinnie? Jeff? Head to Jekyll Island so you can experience QUALITY speakers in an island setting. I've heard Jeff Gordon's presentation about Cancer in Hollywood. It's good and a little juicy.( Not quite National Enquirer juicy at the grocery store checkout). You'll be awarded CEU's by NSH. Could it get any better than this?! Students, do you know Robert? If not, get to know him at Jekyll Island. He will be your mentor for a day. Robert will be teaching a HT(ASCP) certification readiness workshop. It's also a good class for people taking the HTL exam. If you haven't received money from you institution yet, please preregister so there will be enough printed hand-outs for the classes. If you have any questions regarding your social activities, please contact me. I do not want to see anyone doing word search puzzles in the lobby or having that glazed look in their eyes. Warm Regards, Billie Z Social director Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From NMargaryan <@t> luriechildrens.org Thu Apr 4 16:23:30 2013 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Thu Apr 4 16:23:37 2013 Subject: [Histonet] IHC (DAB) after H&E? Message-ID: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> Dear Histonetters, I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira From Rcartun <@t> harthosp.org Thu Apr 4 16:48:04 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Apr 4 16:48:19 2013 Subject: [Histonet] IHC (DAB) after H&E? In-Reply-To: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> Message-ID: <515DBCD5.7770.0077.1@harthosp.org> No. Re-hydrating the tissue will remove most (if not all) of the eosin and the hematoxylin will serve as your counterstain (although you may need to counterstain again at the end of you protocol). If you use antigen retrieval for an unstained slide, you will have to use it on the H&E-stained slide to uncover your target. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Margaryan, Naira" 4/4/2013 5:23 PM >>> Dear Histonetters, I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Apr 4 18:26:47 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 4 18:26:51 2013 Subject: [Histonet] Workshop in Texas In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959ED68@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959ED68@CVMMB02.cvm.tamu.edu> Message-ID: May 31 - Columbia, Missouri, presented by Peggy A. Wenk, HTL(ASCP)SLS http://missourihisto.org/Home_Page.html I don't see their brochure on-line, but if you go to page two, you can email the president for more information. June 17 - Las Vegas, Nevada, presented by Robert Lott, HT(ASCP) http://s3.goeshow.com/nsh/SS2013/ereg711677.cfm?pg=home Click on Agenda at the top. If that doesn't work, go to www.nsh.org --> Meeting/Events --> Summer Symposium - click on tabs across top for more information. It's the same presentation at both locations. Robert did all the work on the presentation and handouts, but because he can't present it at all the locations that people want him to present at, he allows me to also present the same workshop. I put in updates when there are changes in ASCP exam guidelines. So big thank you to Robert! NSH webpage also has it on-line, from a 6 hour session that Robert did some years ago. (I don't know how many years ago. The only reason I bring this up is that in all likelihood, some of the information about ASCP Board of Certification will be out of date - their webpages/links have changed, their location and phone numbers of changed, the pass rates of changed, fees have changed, etc.) The basic information about what will be on the exam (e.g., PAS, and how the stain works for example), will be the same. http://www.softconference.com/nsh/slist.asp?C=3971 If that doesn't work, go to www.nsh.org --> Professional Development --> OnLine etc --> Online Learning Center --> Meeting Content --> Career Development Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Bustamante, Lin Sent: Thursday, April 04, 2013 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Workshop in Texas Does anyone knows when this workshop will be offered again?. Maybe on line? Workshop: Are you ready for the HT/HTL exam? Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jean-philippe.berteau <@t> tu-harburg.de Fri Apr 5 02:29:55 2013 From: jean-philippe.berteau <@t> tu-harburg.de (Jean-Philippe Berteau) Date: Fri Apr 5 02:30:34 2013 Subject: [Histonet] stop Message-ID: <000001ce31cf$5eedd800$1cc98800$@tu-harburg.de> I would like to stop being in the list. Thank you for all your good advaces. Cheers Jean -----Message d'origine----- De?: histonet-bounces@lists.utsouthwestern.edu [mailto:] De la part de WILLIAM DESALVO Envoy??: Donnerstag, 4. April 2013 18:54 ??: Akemi Allison; histonet Objet?: RE: [Histonet] CEU's 36 hours in three years. Go to: http://www.ascp.org/Board-of-Certification William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Thu, 4 Apr 2013 09:32:52 -0700 > From: akemiat3377@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CEU's > > Hi All: > > I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry. I am a dinosaur who received her HT in 1968 and my HTL in 1982, so do not need to renew mine. > > Akemi Allison-Tacha, BS, HT (ASCP) HTL > Pathology Manager > Monterey Bay GI Consultants > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > (381) 375-3577 X117 > Email: aallison@montereygi.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Apr 5 04:02:54 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 5 04:04:33 2013 Subject: [Histonet] How to unsubscribe Message-ID: Go to the bottom of any email from Histonet. Under the header ?Histonet Subscribers? ? go to the 2nd non-italics line that starts ?To unsubscribe from Histonet . . . ? - type in your email address where you are receiving the listserv emails - click on ?unsubscribe? - follow rest of directions Also works for when you are going away on vacation ? we don?t want to see ?sorry, I?m out of the office? for the next week. So save this email on your computer, unsubscribe when you go on vacation, re-subscribe when you return. Peggy A. Wenk, HTL(ASCP)SLS From rjbuesa <@t> yahoo.com Fri Apr 5 07:00:40 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 5 07:00:49 2013 Subject: [Histonet] IHC (DAB) after H&E? In-Reply-To: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> Message-ID: <1365163240.74830.YahooMailNeo@web163105.mail.bf1.yahoo.com> Yes, you need to remove it. Hydrate your sections and place them in a 1% aq. acetic acid solution (it will be a weak acid solution) and you will see how the eosin fades and the hematoxylin becomes reddish. Keep them in the solution until almost all the hematoxylin becomes less evident?but it will never totally?"disappear". This will be of minor importance unless the?epitope you are trying to detect is nuclear. ?Ren? J. From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet-request@lists.utsouthwestern.edu" Sent: Thursday, April 4, 2013 5:23 PM Subject: [Histonet] IHC (DAB) after H&E? Dear Histonetters, I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hodges420 <@t> msn.com Fri Apr 5 08:43:44 2013 From: hodges420 <@t> msn.com (MARY T HODGES ) Date: Fri Apr 5 08:43:49 2013 Subject: [Histonet] IHC (DAB) after H&E? Message-ID: I have just ihc over the section and not removed the h/e as long as it is on a positive charged slide and had no problems Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 5 Apr 2013 12:00:40 To: ; ; ; Subject: Re: [Histonet] IHC (DAB) after H&E? Yes, you need to remove it. Hydrate your sections and place them in a 1% aq. acetic acid solution (it will be a weak acid solution) and you will see how the eosin fades and the hematoxylin becomes reddish. Keep them in the solution until almost all the hematoxylin becomes less evident?but it will never totally?"disappear". This will be of minor importance unless the?epitope you are trying to detect is nuclear. ?Ren? J. From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet-request@lists.utsouthwestern.edu" Sent: Thursday, April 4, 2013 5:23 PM Subject: [Histonet] IHC (DAB) after H&E? Dear Histonetters, I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Fri Apr 5 09:14:26 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Fri Apr 5 09:14:33 2013 Subject: [Histonet] IHC (DAB) after H&E? In-Reply-To: References: Message-ID: Same here, we just run down the HE slide back to water and load, the running back to water takes out some of the eosin/hematoxylin in the process.... Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARY T HODGES Sent: Friday, April 05, 2013 8:44 AM To: Rene J Buesa ; NMargaryan@luriechildrens.org ; histonet@lists.utsouthwestern.edu ; histonet-bounces@lists.utsouthwestern.edu ; histonet-request@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC (DAB) after H&E? I have just ihc over the section and not removed the h/e as long as it is on a positive charged slide and had no problems Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 5 Apr 2013 12:00:40 To: ; ; ; Subject: Re: [Histonet] IHC (DAB) after H&E? Yes, you need to remove it. Hydrate your sections and place them in a 1% aq. acetic acid solution (it will be a weak acid solution) and you will see how the eosin fades and the hematoxylin becomes reddish. Keep them in the solution until almost all the hematoxylin becomes less evident?but it will never totally?"disappear". This will be of minor importance unless the?epitope you are trying to detect is nuclear. ?Ren? J. From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet-request@lists.utsouthwestern.edu" Sent: Thursday, April 4, 2013 5:23 PM Subject: [Histonet] IHC (DAB) after H&E? Dear Histonetters, I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Apr 5 10:04:42 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Apr 5 10:03:51 2013 Subject: [Histonet] RE: IHC (DAB) after H&E? In-Reply-To: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA641F70109F@SV-EX08.childrensmemorial.org> Message-ID: We just stain right over the H&E. No problems. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, April 04, 2013 5:24 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] IHC (DAB) after H&E? Dear Histonetters, I would like to perform IHC (DAB) after H&E. Do I need to remove H&E? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From mhale <@t> MiracaLS.com Fri Apr 5 11:35:46 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Fri Apr 5 11:35:54 2013 Subject: [Histonet] KY Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C720D675@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is looking for 2 full time HT/HTL's to join their new laboratory. Candidates must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records This is a full time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From sj_pan <@t> yahoo.com Fri Apr 5 12:19:14 2013 From: sj_pan <@t> yahoo.com (Shirley Pan) Date: Fri Apr 5 12:19:23 2013 Subject: [Histonet] Job Opening Message-ID: <1365182354.57907.YahooMailNeo@web163102.mail.bf1.yahoo.com> Palo Alto Medical Foundation Palo Alto, California Histotechnologist Routine clinic work, IHC, and Special Stains Full Time 4:30 AM-1:00 PM,?M-F Occasional Saturday work contact Shirley Pan pans1@pamf.org From Lori.Disher <@t> HCAhealthcare.com Fri Apr 5 12:37:43 2013 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Fri Apr 5 12:37:49 2013 Subject: [Histonet] Possible CJD and decontaminating Leica ASP300s Message-ID: <778DD853CF606049A37FC2059C8BA07A8ACE7C619F@FWDCWPMSGCMS04.hca.corpad.net> We processed a cell block last week and we are now just being notified that it MAY be a CJD case. Leica tells me there isn't a way to decontaminate my tissue processor. Has anyone else been thru this, and isn't there anything to do besides buy a new Tissue Processor? From BZIMMERM <@t> gru.edu Fri Apr 5 15:03:15 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Apr 5 15:03:30 2013 Subject: [Histonet] Do you know Elizabeth Klar?? Can you do a cartwheel?? Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF7632B1@EX-MLB-03.ad.georgiahealth.edu> Well, folks it's Friday and I'm winding down. Some of you are probably relieved but I had to get one last blurb into the histonet about one of our speakers. Her name is Elizabeth (Ely) Klar and she teaches at Columbus State University in Columbus, Georgia. She will be presenting a workshop on the microscopic identification of the major tissue types. This will be great for students as well as new technicians. (newbies) I know the subject is not one that makes you want to go out and turn cartwheels on the beach, but it is informative and necessary. You want to impress the pathologist when you sit down with him at the double-headed scope. We're looking forward to Ely's presentation and hope to see you there! (or on the beach turning cartwheels) Wanda Simons/bz (you know it!!) President GSH Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From mucram11 <@t> comcast.net Fri Apr 5 15:18:44 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Apr 5 15:19:06 2013 Subject: [Histonet] Arkansas Histologists and Histology Workers Message-ID: <830783291.334273.1365193124053.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Good Afternoon, I am sorry to be doing this by Histonet however; I am the new newsletter editor and we need your help.? We do not have a complete list of all the Histology people for Clinical, Research and Industry in the state or surrounding area and since we are re-introducing the newsletter we need to find you.? We will be using primaily e-mail/PDF files to send the newsletter.??I f we don't have your e-mail please send it to me so I can add you to our list and make sure you get all of the Arkansas Histology News worth printing.? This will keep you update on meetings, membership and how you can help us grow.? If you prefer a hard copy of the news letter then we need an address to send it to so you are not out of the loop. Please help us grow and expand in Arkansas! Pam Marcum From patpxs <@t> gmail.com Fri Apr 5 16:06:35 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Apr 5 16:06:41 2013 Subject: [Histonet] Possible CJD and decontaminating Leica ASP300s In-Reply-To: <778DD853CF606049A37FC2059C8BA07A8ACE7C619F@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A8ACE7C619F@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Lori, You need to contact the National Prion Disease Center at http://www.cjdsurveillance.com/ They will tell you if there is a way to decontaminate anything that may have come in contact with the suspected CJD case. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Apr 5, 2013 at 1:37 PM, wrote: > We processed a cell block last week and we are now just being notified > that it MAY be a CJD case. Leica tells me there isn't a way to > decontaminate my tissue processor. Has anyone else been thru this, and > isn't there anything to do besides buy a new Tissue Processor? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotalk <@t> yahoo.com Fri Apr 5 20:03:50 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Fri Apr 5 20:04:02 2013 Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP In-Reply-To: References: <7B3DEB32E69C034EACB479059C5DE3FF75EDBA@EX-MLB-03.ad.georgiahealth.edu> Message-ID: <1365210230.98198.YahooMailNeo@web121503.mail.ne1.yahoo.com> OK Jack, sounds like another great story when I interview you on HistoTALK! Just don't wear your Mankini! PLEASE! ? Yours, Dave ________________________________ From: Jack Ratliff To: "Zimmerman, Billie" ; Histonet Sent: Thursday, March 28, 2013 3:16 PM Subject: RE: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP PLEASE! PLEASE! PLEASE! I want that room! Oh and since you mentioned speedos and crochet bikinis, you want me to bring my white mankini with the British flag on the front? Just kidding, I think I had better leave that at home! ? Jack Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology 389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 236-4962 (f)jratliff@ratliffhistology.com > From: BZIMMERM@gru.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 28 Mar 2013 18:57:46 +0000 > Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read??? this ASAP > > If you haven't reserved your room for the upcoming GSH meeting next month, please note that all island and standard Oceanside rooms have been reserved.? But,? just because you snoozed, were waiting on money for your institution, or you're just a classic procrastinator, there are still options.? There are upgraded rooms such as the lanai.? This room has a Jacuzzi, microwave, King size bed, small refrigerator, and a private balcony or patio. There's also the efficiency which has the kitchenette with a stove top, refrigerator,? two double beds, sitting area, and private balcony or patio.? The symposium rate still applies and if you have issues or concerns, contact Linda Schepps at Oceanside.? She can't cure procrastination but she can help secure a room for you. > > While you're strolling around the historic area of the island in your speedo or crochet bikini, check out Becky's famous chicken salad.? It's a little place with outside tables.? Just walk up to the window and place your order.? I attempted to have a chicken salad sandwich there but they were all sold out!! The lady managed to let me sample a teaspoon of it. It was delicious and didn't taste like that stuff in the grocery store.? I plan to attempt another order when I return next month!! > > > Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Mon Apr 8 06:42:23 2013 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Mon Apr 8 06:42:32 2013 Subject: [Histonet] Glen Message-ID: http://www.treorealtors.com/includes/newsphoto.php?expect713.php From mlunetta <@t> luhcares.org Mon Apr 8 11:15:11 2013 From: mlunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon Apr 8 11:15:26 2013 Subject: [Histonet] Colorado State Histo Meeting Message-ID: <170CACC1CA297947A168665B532DD05C21ADD4E3@EXLUH01.Ad.luhcares.org> Hi, The 2013 CSH meeting is just two weeks away. If you?re interested in attending the meeting, April 19th & 20th (Fort Collins), but have not registered, please fill out the following online registration form: http://www.coloradohisto.org/reg.aspx You have the option to pay by credit card or by check and payment will be accepted up to and including the day of the meeting. This is a great way to get some needed CE's and network, you never know who might have the controls your looking for. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital From ihcman2010 <@t> hotmail.com Mon Apr 8 12:02:25 2013 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Mon Apr 8 12:02:29 2013 Subject: [Histonet] Glen ignore!!! In-Reply-To: References: Message-ID: All, I did not send this email. My email account has been hacked. Apologies, Glen Dawson > From: ihcman2010@hotmail.com > To: kalschev@svm.vetmed.wisc.edu; jvaughn@mcw.edu; faganma@uwstout.edu; janderson@mcw.edu; dbrigham@mhsjvl.org; histonet@lists.utsouthwestern.edu > Date: Mon, 8 Apr 2013 06:42:23 -0500 > CC: > Subject: [Histonet] Glen > > http://www.treorealtors.com/includes/newsphoto.php?expect713.php > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Mon Apr 8 13:09:06 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Apr 8 13:09:33 2013 Subject: [Histonet] Nail treatment and bone marrow decalcification info needed In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E452F2DB0A1@BFL323E10.pathmdlabs.local> We purchase a solution called Nail Prep from Stat Lab for our nails. It works wonders! You place the nails in the solution for about 1 hour, rinse, and process as usual. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of carmen loiselle Sent: Wednesday, April 03, 2013 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail treatment and bone marrow decalcification info needed Hello fellow histonetters , I need your expertise regarding the treatment of nails. We currently immerse the nails specimen in a solution of tween & formalin up to 2 weeks . We then process and section them. Is there a more rapid and efficient way to do it ??? Also, we recently change our procedure for the fixation of the bone marrow specimen. Up to last week we were using Zenker's solution . Starting in april we are now using the formalin fixation for 24 hours before decalcification. What are the most efficient and rapid way of doing the decalcification without altering the IHC markers currently use for these type of specimen ??? At the present time we are using 10% formic acid for 2 hours, rinse then process. What about RDO agent (which I believe is HCL based) which is faster ???? Thank you in advance for all your responses Carmen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Mon Apr 8 13:08:50 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Apr 8 13:09:57 2013 Subject: [Histonet] GSH Blog Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF764840@EX-MLB-03.ad.georgiahealth.edu> It's about that time. The GSH meeting starts on Friday and I'm looking forward to seeing old friends and meeting new ones. Please stop by and say hello to me. Tonight, I'm working on a few door prizes to put together for the meeting, with the "beach" theme. Wishing everyone safe travels. My daddy always told me, "Slow down, you have a lead foot like your Mama". See you this Friday!! Billie Zimmerman GSH Secretary Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From mhale <@t> MiracaLS.com Mon Apr 8 15:57:54 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Mon Apr 8 15:57:59 2013 Subject: [Histonet] KY HT Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C7210C09@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com> Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From JMitchell <@t> uwhealth.org Mon Apr 8 18:12:50 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Mon Apr 8 18:12:57 2013 Subject: [Histonet] Tri-State Histology Spring Symposium Message-ID: <16F90B93CA23D446980B3D591FD02DAD050550@UWHC-MBX14.uwhis.hosp.wisc.edu> Reminder: Registration is in the last weeks for the Iowa, Wisconsin and Minnesota Tri-State Spring Histology Symposium that will be held at the Hotel Julien, Dubuque, Iowa April 24-26th. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 24th and conclude at noon on Friday April 26th. Join us for a "Roaring Tri-State Return to Dubuque" in 2013. For program, registration and vendor/exhibit information contact the following representatives: Iowa: Judi Stasko (judith.stasko@ars.usda.gov) Wisconsin: Jean Mitchell (jmitchell@uwhealth.org) Minnesota: Sheri Blair (sheriblair1@netzero.net) For the last couple of Vendor/Exhibit spots remaining contact: Dawn Schneider (dawn.schneider@ministryhealth.org) From mbmphoto <@t> gmail.com Mon Apr 8 21:24:30 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Apr 8 21:24:39 2013 Subject: [Histonet] need sales rep. for HM340E rotary microtome In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD050550@UWHC-MBX14.uwhis.hosp.wisc.edu> References: <16F90B93CA23D446980B3D591FD02DAD050550@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: Please would a sales rep. from the Microm company or Thermo Scientific contact me. I'm interested in a HM340E rotary microtome. Regards Maria Mejia Histology Supervisor Affymetrix, Inc From mhale <@t> MiracaLS.com Tue Apr 9 07:41:28 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Apr 9 07:41:33 2013 Subject: [Histonet] Kentucky HT Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C7211706@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com> Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From JWatson <@t> gnf.org Tue Apr 9 09:25:05 2013 From: JWatson <@t> gnf.org (James Watson) Date: Tue Apr 9 09:25:11 2013 Subject: [Histonet] California Society for Histotechnology Meeting May 3-5 Message-ID: Joining the other Societies advertising their meeting on the Histonet. Our meeting this year is in Burlingame near the San Francisco international airport. Our annual symposium is about a month away. Hope that you are planning on joining us in Burlingame. Our new website is still having problems with the online registration, our webmaster is working hard to get it going. But we are not sure when it will be available, so you should consider filling out the attached registration from and mailing it to us. We already have 30 Vendors signed up to attend and have outstanding speakers coming from all over the country with a wide variety of backgrounds and subjects. We are hoping to have another great meeting. See you there. Visit our website at to see the program and mail in registration form. http://www.californiahistology.org/ James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From Mhorne <@t> upei.ca Tue Apr 9 09:28:08 2013 From: Mhorne <@t> upei.ca (Margaret Horne) Date: Tue Apr 9 09:28:19 2013 Subject: [Histonet] gently used Tissue Processor Message-ID: <5163FB48020000D100029869@oes-grpwise.novell.upei.ca> Hi , Is anyone interested in buying a Leica ASP 300 S Tissue Processor. Bought in 2008. used for 1 year. Margaret You can contact me by email : mhorne@upei.ca or phone 902-566-0930 From mbmphoto <@t> gmail.com Tue Apr 9 12:07:12 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Apr 9 12:07:20 2013 Subject: [Histonet] need sales rep. from Microm or Thermo Scientific for microtome Message-ID: <207AB925-F560-4D3A-8C12-45D9B38FEBA4@gmail.com> Would a sales rep from either the Microm company or Thermo Scientific contact me. I'm interested in purchasing a semi microtome with foot pedal. Please contact me ASCP. Regards Maria Mejia San Francisco, CA Affymetrix, Inc From Margaret.Perry <@t> sdstate.edu Tue Apr 9 13:14:13 2013 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Apr 9 13:14:18 2013 Subject: [Histonet] antibody diluent Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE956DFD26@SDSU-EX03.jacks.local> I am looking for a less expensive antibody diluent and would like some opinions on which companies are best . Are there differences between diluents when it comes to background staining? Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From mcauliff <@t> umdnj.edu Tue Apr 9 14:34:12 2013 From: mcauliff <@t> umdnj.edu (Geoff) Date: Tue Apr 9 14:34:16 2013 Subject: [Histonet] antibody diluent In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE956DFD26@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE956DFD26@SDSU-EX03.jacks.local> Message-ID: <51646D34.9070007@umdnj.edu> You could make your own. It is usually just a buffer with some normal serum and Triton or Tween added. Geoff On 4/9/2013 2:14 PM, Perry, Margaret wrote: > I am looking for a less expensive antibody diluent and would like some opinions on which companies are best . Are there differences between diluents when it comes to background staining? > > Margaret Perry HT(ASCP) > Veterinary & Biomedical Sciences Department > North Campus Drive Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From eca9 <@t> georgetown.edu Tue Apr 9 14:37:31 2013 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Tue Apr 9 14:38:07 2013 Subject: [Histonet] antibody diluent In-Reply-To: <51646D34.9070007@umdnj.edu> References: <25F4FBA34BE9D142964ECC4525B82AEE956DFD26@SDSU-EX03.jacks.local> <51646D34.9070007@umdnj.edu> Message-ID: We usually just use 1xTris buffered saline with 0.05% Tween. Eva On Tue, Apr 9, 2013 at 3:34 PM, Geoff wrote: > You could make your own. It is usually just a buffer with some normal > serum and Triton or Tween added. > > Geoff > > > On 4/9/2013 2:14 PM, Perry, Margaret wrote: > >> I am looking for a less expensive antibody diluent and would like some >> opinions on which companies are best . Are there differences between >> diluents when it comes to background staining? >> >> Margaret Perry HT(ASCP) >> Veterinary & Biomedical Sciences Department >> North Campus Drive Box 2175 >> South Dakota State University >> Brookings SD 57007 >> 605-688-5638 >> >> ______________________________**_________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.**edu >> http://lists.utsouthwestern.**edu/mailman/listinfo/histonet >> > > > -- > -- > ************************************************ > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ************************************************ > > > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Tue Apr 9 15:01:48 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 9 15:01:54 2013 Subject: [Histonet] antibody diluent In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE956DFD26@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE956DFD26@SDSU-EX03.jacks.local> Message-ID: <1365537708.1372.YahooMailNeo@web163102.mail.bf1.yahoo.com> Margaret: Prepare your own Ab diluent, as I did for many many years. Use the follwoing recipe: ? Bovine serum albumen ? 1 mL PBS ---------------------------- 99 mL Tween 20 -------------------- 50 ?L Sodium azide --------------- 100 ?g ? Albumen is a large molecule needed to?prevent the Ab globulin molecules to sink keeping them in a homogeneous suspension. Tween you know is the surfactant helping the Ab to disperse over the sections and Sodium azide is the preservative for the diluent. If you read the label on the?Ab diluent you are using now, you will find the sodium azide at the same proportion in this recipe (0.01%). Keep the diluent in the refrigerator (about 4?C) and it will last for months Ren? J. From: "Perry, Margaret" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, April 9, 2013 2:14 PM Subject: [Histonet] antibody diluent I am looking for a less expensive antibody diluent and would like some opinions on which companies are best .? Are there differences between diluents when it comes to background staining? Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Wed Apr 10 09:14:00 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Wed Apr 10 09:14:07 2013 Subject: [Histonet] GSH Meeting Jekyll Island April 12 - 14 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF7650FA@EX-MLB-03.ad.georgiahealth.edu> The GSH is celebrating 40 years of solid educational opportunities. We look forward to offering phenomenal NSH approved speakers with some "approved" fun thrown in for good measure. Safe travels to all of you. Wanda K. Simons, HT (ASCP) GSH President WS/bz Yes, I agree with Wanda. It will be a great meeting and we're going to have fun. I'm already in "beach mode". I got my toes in the sand.....not a worry in the world, got a cold Coke in my hand.... See ya, Billie Zimmerman MT(ASCP)QIHC Secretary GSH Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From JWatson <@t> gnf.org Wed Apr 10 09:33:54 2013 From: JWatson <@t> gnf.org (James Watson) Date: Wed Apr 10 09:33:59 2013 Subject: [Histonet] RE: California Society for Histotechnology Meeting May 3-5 In-Reply-To: References: Message-ID: I was just informed that On Line registration is now working through our website. http://www.californiahistology.org/ I have also been told this can be accessed by smartphone, I-pad..... also payment can be made through PayPal or Credit Card. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, April 09, 2013 7:25 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] California Society for Histotechnology Meeting May 3-5 Joining the other Societies advertising their meeting on the Histonet. Our meeting this year is in Burlingame near the San Francisco international airport. Our annual symposium is about a month away. Hope that you are planning on joining us in Burlingame. Our new website is still having problems with the online registration, our webmaster is working hard to get it going. But we are not sure when it will be available, so you should consider filling out the attached registration from and mailing it to us. We already have 30 Vendors signed up to attend and have outstanding speakers coming from all over the country with a wide variety of backgrounds and subjects. We are hoping to have another great meeting. See you there. Visit our website at to see the program and mail in registration form. http://www.californiahistology.org/ James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Apr 10 10:19:32 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Apr 10 10:19:36 2013 Subject: [Histonet] RELIA Histology Job Alert 4/10/2013. Some exciting new opportunities and a quick question...Have you joined the social networking craze? Message-ID: <013501ce35fe$ce91f3c0$6bb5db40$@earthlink.net> Hi Histonetters!! Facebook, Google+, Linkedin, Pinterest,Twitter; Have you joined the Social Networking Craze? If so then you know how much fun it can be reconnecting with old friends, making new ones. If you do have an account on one of these networks I would love to connect with you. Please shoot me back an e-mail with your id on the site or an invitation to connect/friend/follow and I will respond right away. If that was like a foreign language to you and you would like some help using any of these sites please let me know. I would be happy to help you join in. I also wanted to tell you about my current job openings. All of these jobs are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Need help with your resume or following up after the interview or negotiating salary? Give me a call or shoot me an e-mail. I would be happy to help out. This Is A Complimentary Service Of RELIA Of Course! Here is a list of my current open positions: HT/HTL - Salem VA Dermpath Histotech - Tyler, Texas HT/HTL Certified Mohs Tech - Long Beach, CA HT/HTL - Nashville, TN Lead Histotech - Atlanta, GA Histotechnician - Atlanta, GA Histology Tech - Collegeville, PA HT/HTL - Louisville, KY NYS Histology Tech - Long Island NYS Histology Tech - Syracuse, NY FISH Tech - Long Island, NY FISH Tech - Philadelphia, PA If you or any of your friends would like more information on any of the positions listed or help with a job search in another area please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jamie.erickson <@t> abbvie.com Wed Apr 10 12:17:16 2013 From: jamie.erickson <@t> abbvie.com (Erickson, Jamie E) Date: Wed Apr 10 12:17:28 2013 Subject: [Histonet] Whole slide scanners, any reviews..? Message-ID: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS From liz <@t> premierlab.com Wed Apr 10 12:45:16 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Apr 10 12:45:15 2013 Subject: [Histonet] RE: Whole slide scanners, any reviews..? In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A83@SBS2K8.premierlab.local> Jamie You can go to the DPA website to get a bit more information on scanners https://digitalpathologyassociation.org/ , there are some white papers up that discuss some different topics and I think the website for http://digitalpathologyconsultants.com/DPCblog/about/ Amanda works for Visopharm which is a software provider but she also has a lot of information on her site. Keith Kaplans site also has a lot of information - http://tissuepathology.com/ There are other scanners that I would considers - Phillips, Hamamatsu, etc. you should look at those too. I know that Hamamatsu also scans fluorescent but not sure about Phillips or Ventana. Leica scanners use Slide Path for their database, I'm not sure what the others use for their database. We have an Aperio ScanScope XT but it only scans brightfield. Some companies that sell scanners also have analysis tool boxes and databases that you can purchase with the scanner and some companies only sell the scanner and you will have to figure out what you are going to do for your database, storage and analysis. If you are looking at analysis and if you are in the clinical setting I would definitely pick a company that has had some of their software 510K cleared for specific analysis (ER/PR, Her2, etc) that will help your internal validation process. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Wednesday, April 10, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners, any reviews..? HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Apr 10 12:48:42 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Apr 10 12:48:39 2013 Subject: [Histonet] RE: Whole slide scanners, any reviews..? In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A84@SBS2K8.premierlab.local> Jamie I realized that you are coming from a pharmaceutical company - for software I would definitely look at Definians or Visiopharm. We have Definians Tissue Studio and have used some of Aperios algorithms also. Visiopharm also has some nice software too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Wednesday, April 10, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners, any reviews..? HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rob <@t> foliobio.com Wed Apr 10 14:53:41 2013 From: rob <@t> foliobio.com (Rob Day) Date: Wed Apr 10 14:54:21 2013 Subject: [Histonet] Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? In-Reply-To: <77DD817201982748BC67D7960F2F76AF023FB6@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF023FB6@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <8D62E288-3E7D-4729-B399-BE6134DAE751@foliobio.com> Greetings histonet, The lab where I work is developing a process to improve IHC and ISH analysis of old, archival FFPE specimens. We are looking for labs who can help us test the effectiveness of this process. If you are having difficulty getting good IHC or ISH results from 10+ or even 20+ year old specimens, let me know, and we will gladly treat some of your slides for free, if you will share the resulting images with us. We posted this request here once before, and we did get some interest, but we are still not convinced that we have adequate independent verification. For more information and some before and after images, go here: http://hematoxylin-eosin-tales.blogspot.com/2013/04/new-technique-for-improving-staining-in.html Rob Day. From jo-ann.bader <@t> mcgill.ca Wed Apr 10 14:54:05 2013 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Wed Apr 10 14:54:32 2013 Subject: [Histonet] Micro-cut H1200 Vibrotome manual Message-ID: <8C36045F0065CE48906E684F15FD4CB6320B9C3E@EXMBX2010-6.campus.MCGILL.CA> Hi Everyone, I am looking for a copy of the manual for the Micro-cut Vibrotome. One of our labs was given one but do not know how to use it. The manual is no long available through Bio-Rad. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From tkngflght <@t> yahoo.com Wed Apr 10 17:24:31 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Apr 10 17:24:35 2013 Subject: [Histonet] Talk to me about shelf life and outdates Message-ID: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'. ? Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? ? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) ? Many thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From liz <@t> premierlab.com Wed Apr 10 17:51:08 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Apr 10 17:51:08 2013 Subject: [Histonet] Talk to me about shelf life and outdates In-Reply-To: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> References: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A8B@SBS2K8.premierlab.local> Cheryl We are not a clinical lab, but we are GLP compliant. This is how we deal with dry chemicals and stains. A lot of times they do not come with an expiration date. If no expiration date/retest date is listed we give them an expiration date/retest date of 5 years post receipt. After that date we place them in a special area that is marked - Past Retest Date - Do not use until retested. We have a reagent control SOP that explains all of this. I'm not familiar with the "Good until used" designation. For retest we just run a special stain that contains a solution prepared from the chemical and if it stains appropriately we give that chemical a new expiration/retest date and just make sure we document the process appropriately. We just initiated this SOP recently so have not yet had to perform any retests. We update our chemical inventory as new chemicals are ordered, but once a year we review and check all our chemicals for inventory and that we have updated MSDS's available. I can send a pdf of our reagent control SOP if you would like it. If you are striving to be a GLP compliant lab and are heading to NSH convention this year I'll be giving a workshop on GLP compliance in the histology lab with our Facility Quality Assurance Manager. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more. They're dated 'Good until used'. Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) Many thanks! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Thu Apr 11 05:19:16 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu Apr 11 05:19:20 2013 Subject: [Histonet] FFPE for Immunohistochemistry. Message-ID: Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB From LBUSTAMANTE <@t> cvm.tamu.edu Thu Apr 11 08:04:21 2013 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Thu Apr 11 08:04:24 2013 Subject: [Histonet] Formalin Neutralizer Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From dencrowl <@t> MIT.EDU Thu Apr 11 08:12:12 2013 From: dencrowl <@t> MIT.EDU (Denise G Crowley) Date: Thu Apr 11 08:12:18 2013 Subject: [Histonet] compression problems Message-ID: <9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1@OC11EXPO25.exchange.mit.edu> Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From cls71877 <@t> gmail.com Thu Apr 11 08:20:49 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Thu Apr 11 08:20:56 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> Message-ID: <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. Thanks, Cristi Sent from my iPhone On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" wrote: > We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. > If you use Formalin Neutralizer, do you have any pro/con about this product? > Thank you very much. > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Apr 11 08:36:00 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Apr 11 08:36:08 2013 Subject: [Histonet] Talk to me about shelf life and outdates In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2A8B@SBS2K8.premierlab.local> References: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AC2A8B@SBS2K8.premierlab.local> Message-ID: We have stains from at least twenty years ago, judging by their labels--they have no date on them! I always wondered how long neutral red lasts, but apparently, it lasts a long, long time. We are not a clincial lab though, we do research. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Apr 10, 2013 at 6:51 PM, Elizabeth Chlipala wrote: > Cheryl > > We are not a clinical lab, but we are GLP compliant. This is how we deal > with dry chemicals and stains. A lot of times they do not come with an > expiration date. If no expiration date/retest date is listed we give them > an expiration date/retest date of 5 years post receipt. After that date > we place them in a special area that is marked - Past Retest Date - Do not > use until retested. We have a reagent control SOP that explains all of > this. I'm not familiar with the "Good until used" designation. For retest > we just run a special stain that contains a solution prepared from the > chemical and if it stains appropriately we give that chemical a new > expiration/retest date and just make sure we document the process > appropriately. We just initiated this SOP recently so have not yet had to > perform any retests. We update our chemical inventory as new chemicals > are ordered, but once a year we review and check all our chemicals for > inventory and that we have updated MSDS's available. I can send a pdf of > our reagent control SOP if you would like it. If you are striving to be a > GLP compliant lab and are heading to NSH convention this year I'll be > giving a workshop on GLP compliance in the histology lab with our Facility > Quality Assurance Manager. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Wednesday, April 10, 2013 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Talk to me about shelf life and outdates > > We're prepping for an inspection and I have a small amount of older > powdered dyes and substances we can't get any more. They're dated 'Good > until used'. > > Would someone elaborate on how we came to have outdates on things that > never had them before, and how those outdates are determined? > > Is there a procedure or policy you might share so we can keep our old but > good items in service until we run out rather than in 12 months (EEEK!!!) > > Many thanks! > > Cheryl > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and > current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and specialty > in the body of the email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Thu Apr 11 09:18:53 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 11 09:18:53 2013 Subject: [Histonet] RE: compression problems In-Reply-To: <9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1@OC11EXPO25.exchange.mit.edu> References: <9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1@OC11EXPO25.exchange.mit.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A98@SBS2K8.premierlab.local> Denise We use paraplast and paraplast extra and have not experienced any problems. We just got a new lot of paraplast extra in and so far everything seems ok. I would check to see if it's a particular lot number that you are using. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Apr 11 09:19:29 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Apr 11 09:19:36 2013 Subject: [Histonet] Talk to me about shelf life and outdates In-Reply-To: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> References: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> Message-ID: We also have some ancient dyes in powder form that we label "Discard at Loss of Function". We were audited recently and there was no problem with the labeling, just as long as everything had a label. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'. ? Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? ? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) ? Many thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Apr 11 09:58:04 2013 From: mcauliff <@t> umdnj.edu (Geoff) Date: Thu Apr 11 09:58:23 2013 Subject: [Histonet] FFPE for Immunohistochemistry. In-Reply-To: References: Message-ID: <5166CF7C.7080202@umdnj.edu> It depends on the antigen. I know that sounds like a cop out but it is true. Review the literature covering the antigen you are looking for to see what has worked for others Geoff On 4/11/2013 6:19 AM, Ian R Bernard wrote: > Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Timothy.Morken <@t> ucsfmedctr.org Thu Apr 11 10:19:49 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Apr 11 10:20:00 2013 Subject: [Histonet] RE: FFPE for Immunohistochemistry. In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0701B9@ex07.net.ucsf.edu> Generally under- fixation in formalin is more of a problem than longer fixation. The reason is first that less fixation does not preserve the tissue and antigens as well (more extraction of proteins in the processing steps), and second, antigen retrieval of any type is more damaging to under-fixed tissue. Studies have shown that 6 hours is a minimum to achieve formalin cross-linking of peptides and inactivate enzymes. After that time period antigen retrieval will be reproducible. Longer fixation is ok. Many studies have shown good antigen retrieval for the vast majority of antigens even after months of fixation when buffer-based heat antigen retrieval is used (as opposed to enzyme digestion). An optimum time is probably in the neighborhood of 16 to 24 hours. But most labs don't do that (clinical labs) and usually 6-12 hours fixation is used. Her2 and ER/PR guidelines specify minimum 6 hours fixation for bx cores and trimmed tissue. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, April 11, 2013 3:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE for Immunohistochemistry. Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elizabeth.Cameron <@t> jax.org Thu Apr 11 10:21:22 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Thu Apr 11 10:21:32 2013 Subject: [Histonet] Anti-human nuclear antibody Message-ID: Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Elizabeth.Cameron <@t> jax.org Thu Apr 11 10:22:55 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Thu Apr 11 10:23:05 2013 Subject: [Histonet] RE: Anti-human nuclear antibody Message-ID: Forgot to mention this is on FFPE tissue.... From: Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From relia1 <@t> earthlink.net Thu Apr 11 10:41:07 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Apr 11 10:41:13 2013 Subject: [Histonet] Histology Society of Ohio Symposium/Convention April 26-27 Message-ID: <00d201ce36ca$fdeacbc0$f9c06340$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day. I was asked by a friend to forward this information to you about the Histology Society of Ohio's Symposium/Convention which will be held April 26-27 2013 at the Holiday Inn Cleveland- West For more info and the program schedule please go to www.ohiohistology.org Histology Society of Ohio Symposium/Convention April 26-27, 2013 Holiday Inn Cleveland-West Westlake, OH www.ohiohistology.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mbmphoto <@t> gmail.com Thu Apr 11 11:10:17 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Apr 11 11:10:24 2013 Subject: [Histonet] HM 355S microtome users Message-ID: Is anyone currently using the HM 355S full automatic rotary microtome? Please can anyone give me a review of this particular microtome - pros & cons would be very helpful from those individuals actually using this machine on a daily basis. My lab is considering purchasing this microtome. I would be most grateful for any assistance users can provide. Best Maria Mejia Affymetrix, Inc From AGleiberman <@t> cbiolabs.com Thu Apr 11 11:43:05 2013 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Apr 11 11:43:27 2013 Subject: [Histonet] RE: Anti-human nuclear antibody In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C016DC952F3@cbiolabs05.CBiolabs.local> Elizabeth, We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03), Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From JCBRITTON <@t> Cheshire-Med.COM Thu Apr 11 12:08:07 2013 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Thu Apr 11 12:08:18 2013 Subject: [Histonet] compression problems In-Reply-To: <9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1@OC11EXPO25.exchange.mit.edu> Message-ID: We had this happen to us before. We had a bad lot of blades! Josie Britton HT QIHC (ASCP) Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Wanda.Smith <@t> HCAhealthcare.com Thu Apr 11 12:52:30 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Apr 11 12:52:41 2013 Subject: [Histonet] HM 355S microtome users In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D62770F6@NADCWPMSGCMS03.hca.corpad.net> I have an old HM 355S and a fairly new one. We love them and they are excellent microtomes that cut well. I would buy another one if the funds ever become available. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Mejia Sent: Thursday, April 11, 2013 12:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HM 355S microtome users Is anyone currently using the HM 355S full automatic rotary microtome? Please can anyone give me a review of this particular microtome - pros & cons would be very helpful from those individuals actually using this machine on a daily basis. My lab is considering purchasing this microtome. I would be most grateful for any assistance users can provide. Best Maria Mejia Affymetrix, Inc_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sae2001 <@t> med.cornell.edu Thu Apr 11 13:05:29 2013 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Thu Apr 11 13:06:28 2013 Subject: [Histonet] Whole slide scanners. Message-ID: I have worked with whole slide scanners and digital image analysis since 1998. We currently own scanners from 3 different vendors. Also, I have participated in whole slide scanner marketing research, sitting at a discussion table with a dozen people from different hospitals. My experience has been that most departments purchase scanners without a clear need. For example, some want to scan and archive slides, to make them available for review. That makes some sense in theory. However, in every institution I've talked to, they end up paying for the scanner, a tech to do the work, the service contract and the server space to store the images, which can be huge, up to 20 *giga*bytes per *slide... then find that it is rare for anyone to ever actually look at the scanned images. Most pathologists find it is more expedient to simply pull the glass slides out of the archive. In New York, we are required by law to archive glass slides for at least 10 years anyway. So, my advice, for what it's worth, is not to purchase any whole slide scanner unless you have a clear and well defined need (we have 3 giant systems, rarely used, taking up space and gathering dust). For example, if you need to store images from consultation slides before returning them to another institution, why not just snap a few photomicrographs? Being able to view a couple of small, representative pictures is better than having to wade through an entire scanned slide. ------------------------------------------------------------------------------- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------------------------------------------------------- ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, April 11, 2013 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Whole slide scanners, any reviews..? (Erickson, Jamie E) 2. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 3. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 4. Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? (Rob Day) 5. Micro-cut H1200 Vibrotome manual (Jo-Ann Bader, Ms.) 6. Talk to me about shelf life and outdates (Cheryl) 7. RE: Talk to me about shelf life and outdates (Elizabeth Chlipala) 8. FFPE for Immunohistochemistry. (Ian R Bernard) 9. Formalin Neutralizer (Bustamante, Lin) 10. compression problems (Denise G Crowley) 11. Re: Formalin Neutralizer (Cristi Rigazio) 12. Re: Talk to me about shelf life and outdates (Emily Sours) 13. RE: compression problems (Elizabeth Chlipala) 14. RE: Talk to me about shelf life and outdates (Goins, Tresa) 15. Re: FFPE for Immunohistochemistry. (Geoff) 16. RE: FFPE for Immunohistochemistry. (Morken, Timothy) 17. Anti-human nuclear antibody (Elizabeth Cameron) 18. RE: Anti-human nuclear antibody (Elizabeth Cameron) 19. Histology Society of Ohio Symposium/Convention April 26-27 (Pam Barker) 20. HM 355S microtome users (Maria Mejia) 21. RE: Anti-human nuclear antibody (Anatoli Gleiberman) ---------------------------------------------------------------------- Message: 1 Date: Wed, 10 Apr 2013 17:17:16 +0000 From: "Erickson, Jamie E" Subject: [Histonet] Whole slide scanners, any reviews..? To: "histonet@lists.utsouthwestern.edu" Message-ID: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> Content-Type: text/plain; charset="us-ascii" HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS ------------------------------ Message: 2 Date: Wed, 10 Apr 2013 11:45:16 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Whole slide scanners, any reviews..? To: "Erickson, Jamie E" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A83@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Jamie You can go to the DPA website to get a bit more information on scanners https://digitalpathologyassociation.org/ , there are some white papers up that discuss some different topics and I think the website for http://digitalpathologyconsultants.com/DPCblog/about/ Amanda works for Visopharm which is a software provider but she also has a lot of information on her site. Keith Kaplans site also has a lot of information - http://tissuepathology.com/ There are other scanners that I would considers - Phillips, Hamamatsu, etc. you should look at those too. I know that Hamamatsu also scans fluorescent but not sure about Phillips or Ventana. Leica scanners use Slide Path for their database, I'm not sure what the others use for their database. We have an Aperio ScanScope XT but it only scans brightfield. Some companies that sell scanners also have analysis tool boxes and databases that you can purchase with the scanner and some companies only sell the scanner and you will have to figure out what you are going to do for your database, storage and analysis. If you are looking at analysis and if you are in the clinical setting I would definitely pick a company that has had some of their software 510K cleared for specific analysis (ER/PR, Her2, etc) that will help your internal validation process. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Wednesday, April 10, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners, any reviews..? HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 10 Apr 2013 11:48:42 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Whole slide scanners, any reviews..? To: "Erickson, Jamie E" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A84@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Jamie I realized that you are coming from a pharmaceutical company - for software I would definitely look at Definians or Visiopharm. We have Definians Tissue Studio and have used some of Aperios algorithms also. Visiopharm also has some nice software too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Wednesday, April 10, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners, any reviews..? HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 10 Apr 2013 15:53:41 -0400 From: Rob Day Subject: [Histonet] Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? To: "histonet@lists.utsouthwestern.edu" Message-ID: <8D62E288-3E7D-4729-B399-BE6134DAE751@foliobio.com> Content-Type: text/plain; charset=us-ascii Greetings histonet, The lab where I work is developing a process to improve IHC and ISH analysis of old, archival FFPE specimens. We are looking for labs who can help us test the effectiveness of this process. If you are having difficulty getting good IHC or ISH results from 10+ or even 20+ year old specimens, let me know, and we will gladly treat some of your slides for free, if you will share the resulting images with us. We posted this request here once before, and we did get some interest, but we are still not convinced that we have adequate independent verification. For more information and some before and after images, go here: http://hematoxylin-eosin-tales.blogspot.com/2013/04/new-technique-for-improving-staining-in.html Rob Day. ------------------------------ Message: 5 Date: Wed, 10 Apr 2013 19:54:05 +0000 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Micro-cut H1200 Vibrotome manual To: "histonet@lists.utsouthwestern.edu" Message-ID: <8C36045F0065CE48906E684F15FD4CB6320B9C3E@EXMBX2010-6.campus.MCGILL.CA> Content-Type: text/plain; charset="iso-8859-1" Hi Everyone, I am looking for a copy of the manual for the Micro-cut Vibrotome. One of our labs was given one but do not know how to use it. The manual is no long available through Bio-Rad. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ------------------------------ Message: 6 Date: Wed, 10 Apr 2013 15:24:31 -0700 (PDT) From: Cheryl Subject: [Histonet] Talk to me about shelf life and outdates To: histonet@lists.utsouthwestern.edu Message-ID: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'. ? Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? ? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) ? Many thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. ------------------------------ Message: 7 Date: Wed, 10 Apr 2013 16:51:08 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] Talk to me about shelf life and outdates To: Cheryl , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A8B@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Cheryl We are not a clinical lab, but we are GLP compliant. This is how we deal with dry chemicals and stains. A lot of times they do not come with an expiration date. If no expiration date/retest date is listed we give them an expiration date/retest date of 5 years post receipt. After that date we place them in a special area that is marked - Past Retest Date - Do not use until retested. We have a reagent control SOP that explains all of this. I'm not familiar with the "Good until used" designation. For retest we just run a special stain that contains a solution prepared from the chemical and if it stains appropriately we give that chemical a new expiration/retest date and just make sure we document the process appropriately. We just initiated this SOP recently so have not yet had to perform any retests. We update our chemical inventory as new chemicals are ordered, but once a year we review and check all our chemicals for inventory and that we have updated MSDS's available. I can send a pdf of our reagent control SOP if you would like it. If you are striving to be a GLP compliant lab and are heading to NSH convention this year I'll be giving a workshop on GLP compliance in the histology lab with our Facility Quality Assurance Manager. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more. They're dated 'Good until used'. Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) Many thanks! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 11 Apr 2013 10:19:16 +0000 From: Ian R Bernard Subject: [Histonet] FFPE for Immunohistochemistry. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB ------------------------------ Message: 9 Date: Thu, 11 Apr 2013 13:04:21 +0000 From: "Bustamante, Lin" Subject: [Histonet] Formalin Neutralizer To: "histonet@lists.utsouthwestern.edu" Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> Content-Type: text/plain; charset="us-ascii" We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ------------------------------ Message: 10 Date: Thu, 11 Apr 2013 13:12:12 +0000 From: Denise G Crowley Subject: [Histonet] compression problems To: "histonet@lists.utsouthwestern.edu" Message-ID: <9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1@OC11EXPO25.exchange.mit.edu> Content-Type: text/plain; charset="us-ascii" Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu ------------------------------ Message: 11 Date: Thu, 11 Apr 2013 06:20:49 -0700 From: Cristi Rigazio Subject: Re: [Histonet] Formalin Neutralizer To: "Bustamante, Lin" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> Content-Type: text/plain; charset=us-ascii We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. Thanks, Cristi Sent from my iPhone On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" wrote: > We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. > If you use Formalin Neutralizer, do you have any pro/con about this product? > Thank you very much. > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 11 Apr 2013 09:36:00 -0400 From: Emily Sours Subject: Re: [Histonet] Talk to me about shelf life and outdates Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 We have stains from at least twenty years ago, judging by their labels--they have no date on them! I always wondered how long neutral red lasts, but apparently, it lasts a long, long time. We are not a clincial lab though, we do research. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Apr 10, 2013 at 6:51 PM, Elizabeth Chlipala wrote: > Cheryl > > We are not a clinical lab, but we are GLP compliant. This is how we deal > with dry chemicals and stains. A lot of times they do not come with an > expiration date. If no expiration date/retest date is listed we give them > an expiration date/retest date of 5 years post receipt. After that date > we place them in a special area that is marked - Past Retest Date - Do not > use until retested. We have a reagent control SOP that explains all of > this. I'm not familiar with the "Good until used" designation. For retest > we just run a special stain that contains a solution prepared from the > chemical and if it stains appropriately we give that chemical a new > expiration/retest date and just make sure we document the process > appropriately. We just initiated this SOP recently so have not yet had to > perform any retests. We update our chemical inventory as new chemicals > are ordered, but once a year we review and check all our chemicals for > inventory and that we have updated MSDS's available. I can send a pdf of > our reagent control SOP if you would like it. If you are striving to be a > GLP compliant lab and are heading to NSH convention this year I'll be > giving a workshop on GLP compliance in the histology lab with our Facility > Quality Assurance Manager. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Wednesday, April 10, 2013 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Talk to me about shelf life and outdates > > We're prepping for an inspection and I have a small amount of older > powdered dyes and substances we can't get any more. They're dated 'Good > until used'. > > Would someone elaborate on how we came to have outdates on things that > never had them before, and how those outdates are determined? > > Is there a procedure or policy you might share so we can keep our old but > good items in service until we run out rather than in 12 months (EEEK!!!) > > Many thanks! > > Cheryl > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and > current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and specialty > in the body of the email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Thu, 11 Apr 2013 08:18:53 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: compression problems To: Denise G Crowley , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A98@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Denise We use paraplast and paraplast extra and have not experienced any problems. We just got a new lot of paraplast extra in and so far everything seems ok. I would check to see if it's a particular lot number that you are using. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 11 Apr 2013 14:19:29 +0000 From: "Goins, Tresa" Subject: RE: [Histonet] Talk to me about shelf life and outdates To: Cheryl , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We also have some ancient dyes in powder form that we label "Discard at Loss of Function". We were audited recently and there was no problem with the labeling, just as long as everything had a label. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'. ? Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? ? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) ? Many thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 11 Apr 2013 10:58:04 -0400 From: Geoff Subject: Re: [Histonet] FFPE for Immunohistochemistry. To: histonet@lists.utsouthwestern.edu Message-ID: <5166CF7C.7080202@umdnj.edu> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed It depends on the antigen. I know that sounds like a cop out but it is true. Review the literature covering the antigen you are looking for to see what has worked for others Geoff On 4/11/2013 6:19 AM, Ian R Bernard wrote: > Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 16 Date: Thu, 11 Apr 2013 15:19:49 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: FFPE for Immunohistochemistry. To: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF0701B9@ex07.net.ucsf.edu> Content-Type: text/plain; charset=us-ascii Generally under- fixation in formalin is more of a problem than longer fixation. The reason is first that less fixation does not preserve the tissue and antigens as well (more extraction of proteins in the processing steps), and second, antigen retrieval of any type is more damaging to under-fixed tissue. Studies have shown that 6 hours is a minimum to achieve formalin cross-linking of peptides and inactivate enzymes. After that time period antigen retrieval will be reproducible. Longer fixation is ok. Many studies have shown good antigen retrieval for the vast majority of antigens even after months of fixation when buffer-based heat antigen retrieval is used (as opposed to enzyme digestion). An optimum time is probably in the neighborhood of 16 to 24 hours. But most labs don't do that (clinical labs) and usually 6-12 hours fixation is used. Her2 and ER/PR guidelines specify minimum 6 hours fixation for bx cores and trimmed tissue. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, April 11, 2013 3:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE for Immunohistochemistry. Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 11 Apr 2013 15:21:22 +0000 From: Elizabeth Cameron Subject: [Histonet] Anti-human nuclear antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ Message: 18 Date: Thu, 11 Apr 2013 15:22:55 +0000 From: Elizabeth Cameron Subject: [Histonet] RE: Anti-human nuclear antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Forgot to mention this is on FFPE tissue.... From: Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ Message: 19 Date: Thu, 11 Apr 2013 11:41:07 -0400 From: "Pam Barker" Subject: [Histonet] Histology Society of Ohio Symposium/Convention April 26-27 To: "Histonet" Message-ID: <00d201ce36ca$fdeacbc0$f9c06340$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! I hope everyone is having a great day. I was asked by a friend to forward this information to you about the Histology Society of Ohio's Symposium/Convention which will be held April 26-27 2013 at the Holiday Inn Cleveland- West For more info and the program schedule please go to www.ohiohistology.org Histology Society of Ohio Symposium/Convention April 26-27, 2013 Holiday Inn Cleveland-West Westlake, OH www.ohiohistology.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 20 Date: Thu, 11 Apr 2013 09:10:17 -0700 From: Maria Mejia Subject: [Histonet] HM 355S microtome users To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Is anyone currently using the HM 355S full automatic rotary microtome? Please can anyone give me a review of this particular microtome - pros & cons would be very helpful from those individuals actually using this machine on a daily basis. My lab is considering purchasing this microtome. I would be most grateful for any assistance users can provide. Best Maria Mejia Affymetrix, Inc ------------------------------ Message: 21 Date: Thu, 11 Apr 2013 16:43:05 +0000 From: Anatoli Gleiberman Subject: [Histonet] RE: Anti-human nuclear antibody To: Elizabeth Cameron , "histonet@lists.utsouthwestern.edu" Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C016DC952F3@cbiolabs05.CBiolabs.local> Content-Type: text/plain; charset="us-ascii" Elizabeth, We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03), Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 10 ***************************************** From cjbulmer <@t> sbcglobal.net Thu Apr 11 13:07:27 2013 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Thu Apr 11 13:07:31 2013 Subject: [Histonet] control slides Message-ID: <1365703647.92994.YahooMailRC@web181103.mail.ne1.yahoo.com> Hello, Does anyone have a good source for buying HSV control slides for IHC? Thank you, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From MDiCarlo <@t> KaleidaHealth.Org Thu Apr 11 13:43:32 2013 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Apr 11 13:43:39 2013 Subject: [Histonet] 35 mm scanners Message-ID: <731BE09CDB19AA43AA8682199D42D31B0547121E@ADCEXCHANGE01.KaleidaHealth.org> Everyone, I currently use a Nikon Digital Ice 3, Coolscan IV ED to scan 35 mm photos. Lately, it is often scanning a magenta color or at times either a faded or blurry image and I know I will need to replace it soon. Can anyone recommend a reasonably priced, good quality 35 scanner? It seems they are more difficult to find these days. Your advice would greatly be appreciated. Thank you. Peggy DiCarlo HT(ASCP) Ortho Bone Lab Buffalo General Hospital 100 High Street Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂ’s Technology Assistance Center at (716) 859-7777. From Aliete_Langsdorf <@t> MEEI.HARVARD.EDU Thu Apr 11 14:29:50 2013 From: Aliete_Langsdorf <@t> MEEI.HARVARD.EDU (Langsdorf, Aliete) Date: Thu Apr 11 14:30:04 2013 Subject: [Histonet] PAP Pen With Aqueous Mounting Media Message-ID: <84B03145D9971342951CDF0E003F8874165860@isvifmbxsvr02.meei.harvard.edu> Hello All, I would like to use a PAP pen to separate sections on my slide, but have been using an aqueous (non-xylenes based) mounting media. How do you remove the PAP pen without using xylenes? Is this done? Thank you, ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye & Ear Infirmary Lab: (617)-573-6485 From brett_connolly <@t> merck.com Thu Apr 11 14:46:02 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Apr 11 14:46:10 2013 Subject: [Histonet] RE: Whole slide scanners. In-Reply-To: References: Message-ID: I think Scott makes a good point about having a clear need. We use the Vectra system now, and have previously used Ariol. We only scan IHC slides from projects in which we want a quantitative analysis. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott A. Ely Sent: Thursday, April 11, 2013 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners. I have worked with whole slide scanners and digital image analysis since 1998. We currently own scanners from 3 different vendors. Also, I have participated in whole slide scanner marketing research, sitting at a discussion table with a dozen people from different hospitals. My experience has been that most departments purchase scanners without a clear need. For example, some want to scan and archive slides, to make them available for review. That makes some sense in theory. However, in every institution I've talked to, they end up paying for the scanner, a tech to do the work, the service contract and the server space to store the images, which can be huge, up to 20 *giga*bytes per *slide... then find that it is rare for anyone to ever actually look at the scanned images. Most pathologists find it is more expedient to simply pull the glass slides out of the archive. In New York, we are required by law to archive glass slides for at least 10 years anyway. So, my advice, for what it's worth, is not to purchase any whole slide scanner unless you have a clear and well defined need (we have 3 giant systems, rarely used, taking up space and gathering dust). For example, if you need to store images from consultation slides before returning them to another institution, why not just snap a few photomicrographs? Being able to view a couple of small, representative pictures is better than having to wade through an entire scanned slide. ------------------------------------------------------------------------------- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------------------------------------------------------- ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, April 11, 2013 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Whole slide scanners, any reviews..? (Erickson, Jamie E) 2. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 3. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 4. Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? (Rob Day) 5. Micro-cut H1200 Vibrotome manual (Jo-Ann Bader, Ms.) 6. Talk to me about shelf life and outdates (Cheryl) 7. RE: Talk to me about shelf life and outdates (Elizabeth Chlipala) 8. FFPE for Immunohistochemistry. (Ian R Bernard) 9. Formalin Neutralizer (Bustamante, Lin) 10. compression problems (Denise G Crowley) 11. Re: Formalin Neutralizer (Cristi Rigazio) 12. Re: Talk to me about shelf life and outdates (Emily Sours) 13. RE: compression problems (Elizabeth Chlipala) 14. RE: Talk to me about shelf life and outdates (Goins, Tresa) 15. Re: FFPE for Immunohistochemistry. (Geoff) 16. RE: FFPE for Immunohistochemistry. (Morken, Timothy) 17. Anti-human nuclear antibody (Elizabeth Cameron) 18. RE: Anti-human nuclear antibody (Elizabeth Cameron) 19. Histology Society of Ohio Symposium/Convention April 26-27 (Pam Barker) 20. HM 355S microtome users (Maria Mejia) 21. RE: Anti-human nuclear antibody (Anatoli Gleiberman) ---------------------------------------------------------------------- Message: 1 Date: Wed, 10 Apr 2013 17:17:16 +0000 From: "Erickson, Jamie E" Subject: [Histonet] Whole slide scanners, any reviews..? To: "histonet@lists.utsouthwestern.edu" Message-ID: <8B946A68A8F3534A99CC493DEFB49B10131DD88D@WM10002P.oneabbott.com> Content-Type: text/plain; charset="us-ascii" HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS ------------------------------ Message: 2 Date: Wed, 10 Apr 2013 11:45:16 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Whole slide scanners, any reviews..? To: "Erickson, Jamie E" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A83@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Jamie You can go to the DPA website to get a bit more information on scanners https://digitalpathologyassociation.org/ , there are some white papers up that discuss some different topics and I think the website for http://digitalpathologyconsultants.com/DPCblog/about/ Amanda works for Visopharm which is a software provider but she also has a lot of information on her site. Keith Kaplans site also has a lot of information - http://tissuepathology.com/ There are other scanners that I would considers - Phillips, Hamamatsu, etc. you should look at those too. I know that Hamamatsu also scans fluorescent but not sure about Phillips or Ventana. Leica scanners use Slide Path for their database, I'm not sure what the others use for their database. We have an Aperio ScanScope XT but it only scans brightfield. Some companies that sell scanners also have analysis tool boxes and databases that you can purchase with the scanner and some companies only sell the scanner and you will have to figure out what you are going to do for your database, storage and analysis. If you are looking at analysis and if you are in the clinical setting I would definitely pick a company that has had some of their software 510K cleared for specific analysis (ER/PR, Her2, etc) that will help your internal validation process. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Wednesday, April 10, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners, any reviews..? HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 10 Apr 2013 11:48:42 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Whole slide scanners, any reviews..? To: "Erickson, Jamie E" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A84@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Jamie I realized that you are coming from a pharmaceutical company - for software I would definitely look at Definians or Visiopharm. We have Definians Tissue Studio and have used some of Aperios algorithms also. Visiopharm also has some nice software too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Wednesday, April 10, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners, any reviews..? HI All, Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc. We hope to use this for telepathology as well as image analysis, so any thoughts would be great.. Thanks, Jamie Erickson Scientist II HTL (ASCP),MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 10 Apr 2013 15:53:41 -0400 From: Rob Day Subject: [Histonet] Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? To: "histonet@lists.utsouthwestern.edu" Message-ID: <8D62E288-3E7D-4729-B399-BE6134DAE751@foliobio.com> Content-Type: text/plain; charset=us-ascii Greetings histonet, The lab where I work is developing a process to improve IHC and ISH analysis of old, archival FFPE specimens. We are looking for labs who can help us test the effectiveness of this process. If you are having difficulty getting good IHC or ISH results from 10+ or even 20+ year old specimens, let me know, and we will gladly treat some of your slides for free, if you will share the resulting images with us. We posted this request here once before, and we did get some interest, but we are still not convinced that we have adequate independent verification. For more information and some before and after images, go here: http://hematoxylin-eosin-tales.blogspot.com/2013/04/new-technique-for-improving-staining-in.html Rob Day. ------------------------------ Message: 5 Date: Wed, 10 Apr 2013 19:54:05 +0000 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Micro-cut H1200 Vibrotome manual To: "histonet@lists.utsouthwestern.edu" Message-ID: <8C36045F0065CE48906E684F15FD4CB6320B9C3E@EXMBX2010-6.campus.MCGILL.CA> Content-Type: text/plain; charset="iso-8859-1" Hi Everyone, I am looking for a copy of the manual for the Micro-cut Vibrotome. One of our labs was given one but do not know how to use it. The manual is no long available through Bio-Rad. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ------------------------------ Message: 6 Date: Wed, 10 Apr 2013 15:24:31 -0700 (PDT) From: Cheryl Subject: [Histonet] Talk to me about shelf life and outdates To: histonet@lists.utsouthwestern.edu Message-ID: <1365632671.80304.YahooMailClassic@web161906.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'. ? Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? ? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) ? Many thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. ------------------------------ Message: 7 Date: Wed, 10 Apr 2013 16:51:08 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] Talk to me about shelf life and outdates To: Cheryl , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A8B@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Cheryl We are not a clinical lab, but we are GLP compliant. This is how we deal with dry chemicals and stains. A lot of times they do not come with an expiration date. If no expiration date/retest date is listed we give them an expiration date/retest date of 5 years post receipt. After that date we place them in a special area that is marked - Past Retest Date - Do not use until retested. We have a reagent control SOP that explains all of this. I'm not familiar with the "Good until used" designation. For retest we just run a special stain that contains a solution prepared from the chemical and if it stains appropriately we give that chemical a new expiration/retest date and just make sure we document the process appropriately. We just initiated this SOP recently so have not yet had to perform any retests. We update our chemical inventory as new chemicals are ordered, but once a year we review and check all our chemicals for inventory and that we have updated MSDS's available. I can send a pdf of our reagent control SOP if you would like it. If you are striving to be a GLP compliant lab and are heading to NSH convention this year I'll be giving a workshop on GLP compliance in the histology lab with our Facility Quality Assurance Manager. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more. They're dated 'Good until used'. Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) Many thanks! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 11 Apr 2013 10:19:16 +0000 From: Ian R Bernard Subject: [Histonet] FFPE for Immunohistochemistry. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB ------------------------------ Message: 9 Date: Thu, 11 Apr 2013 13:04:21 +0000 From: "Bustamante, Lin" Subject: [Histonet] Formalin Neutralizer To: "histonet@lists.utsouthwestern.edu" Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> Content-Type: text/plain; charset="us-ascii" We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ------------------------------ Message: 10 Date: Thu, 11 Apr 2013 13:12:12 +0000 From: Denise G Crowley Subject: [Histonet] compression problems To: "histonet@lists.utsouthwestern.edu" Message-ID: <9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1@OC11EXPO25.exchange.mit.edu> Content-Type: text/plain; charset="us-ascii" Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu ------------------------------ Message: 11 Date: Thu, 11 Apr 2013 06:20:49 -0700 From: Cristi Rigazio Subject: Re: [Histonet] Formalin Neutralizer To: "Bustamante, Lin" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> Content-Type: text/plain; charset=us-ascii We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. Thanks, Cristi Sent from my iPhone On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" wrote: > We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. > If you use Formalin Neutralizer, do you have any pro/con about this product? > Thank you very much. > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 11 Apr 2013 09:36:00 -0400 From: Emily Sours Subject: Re: [Histonet] Talk to me about shelf life and outdates Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 We have stains from at least twenty years ago, judging by their labels--they have no date on them! I always wondered how long neutral red lasts, but apparently, it lasts a long, long time. We are not a clincial lab though, we do research. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Apr 10, 2013 at 6:51 PM, Elizabeth Chlipala wrote: > Cheryl > > We are not a clinical lab, but we are GLP compliant. This is how we deal > with dry chemicals and stains. A lot of times they do not come with an > expiration date. If no expiration date/retest date is listed we give them > an expiration date/retest date of 5 years post receipt. After that date > we place them in a special area that is marked - Past Retest Date - Do not > use until retested. We have a reagent control SOP that explains all of > this. I'm not familiar with the "Good until used" designation. For retest > we just run a special stain that contains a solution prepared from the > chemical and if it stains appropriately we give that chemical a new > expiration/retest date and just make sure we document the process > appropriately. We just initiated this SOP recently so have not yet had to > perform any retests. We update our chemical inventory as new chemicals > are ordered, but once a year we review and check all our chemicals for > inventory and that we have updated MSDS's available. I can send a pdf of > our reagent control SOP if you would like it. If you are striving to be a > GLP compliant lab and are heading to NSH convention this year I'll be > giving a workshop on GLP compliance in the histology lab with our Facility > Quality Assurance Manager. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Wednesday, April 10, 2013 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Talk to me about shelf life and outdates > > We're prepping for an inspection and I have a small amount of older > powdered dyes and substances we can't get any more. They're dated 'Good > until used'. > > Would someone elaborate on how we came to have outdates on things that > never had them before, and how those outdates are determined? > > Is there a procedure or policy you might share so we can keep our old but > good items in service until we run out rather than in 12 months (EEEK!!!) > > Many thanks! > > Cheryl > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and > current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and specialty > in the body of the email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Thu, 11 Apr 2013 08:18:53 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: compression problems To: Denise G Crowley , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2A98@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Denise We use paraplast and paraplast extra and have not experienced any problems. We just got a new lot of paraplast extra in and so far everything seems ok. I would check to see if it's a particular lot number that you are using. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 11 Apr 2013 14:19:29 +0000 From: "Goins, Tresa" Subject: RE: [Histonet] Talk to me about shelf life and outdates To: Cheryl , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We also have some ancient dyes in powder form that we label "Discard at Loss of Function". We were audited recently and there was no problem with the labeling, just as long as everything had a label. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'. ? Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? ? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) ? Many thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 11 Apr 2013 10:58:04 -0400 From: Geoff Subject: Re: [Histonet] FFPE for Immunohistochemistry. To: histonet@lists.utsouthwestern.edu Message-ID: <5166CF7C.7080202@umdnj.edu> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed It depends on the antigen. I know that sounds like a cop out but it is true. Review the literature covering the antigen you are looking for to see what has worked for others Geoff On 4/11/2013 6:19 AM, Ian R Bernard wrote: > Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 16 Date: Thu, 11 Apr 2013 15:19:49 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: FFPE for Immunohistochemistry. To: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF0701B9@ex07.net.ucsf.edu> Content-Type: text/plain; charset=us-ascii Generally under- fixation in formalin is more of a problem than longer fixation. The reason is first that less fixation does not preserve the tissue and antigens as well (more extraction of proteins in the processing steps), and second, antigen retrieval of any type is more damaging to under-fixed tissue. Studies have shown that 6 hours is a minimum to achieve formalin cross-linking of peptides and inactivate enzymes. After that time period antigen retrieval will be reproducible. Longer fixation is ok. Many studies have shown good antigen retrieval for the vast majority of antigens even after months of fixation when buffer-based heat antigen retrieval is used (as opposed to enzyme digestion). An optimum time is probably in the neighborhood of 16 to 24 hours. But most labs don't do that (clinical labs) and usually 6-12 hours fixation is used. Her2 and ER/PR guidelines specify minimum 6 hours fixation for bx cores and trimmed tissue. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, April 11, 2013 3:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE for Immunohistochemistry. Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 11 Apr 2013 15:21:22 +0000 From: Elizabeth Cameron Subject: [Histonet] Anti-human nuclear antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ Message: 18 Date: Thu, 11 Apr 2013 15:22:55 +0000 From: Elizabeth Cameron Subject: [Histonet] RE: Anti-human nuclear antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Forgot to mention this is on FFPE tissue.... From: Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ Message: 19 Date: Thu, 11 Apr 2013 11:41:07 -0400 From: "Pam Barker" Subject: [Histonet] Histology Society of Ohio Symposium/Convention April 26-27 To: "Histonet" Message-ID: <00d201ce36ca$fdeacbc0$f9c06340$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! I hope everyone is having a great day. I was asked by a friend to forward this information to you about the Histology Society of Ohio's Symposium/Convention which will be held April 26-27 2013 at the Holiday Inn Cleveland- West For more info and the program schedule please go to www.ohiohistology.org Histology Society of Ohio Symposium/Convention April 26-27, 2013 Holiday Inn Cleveland-West Westlake, OH www.ohiohistology.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 20 Date: Thu, 11 Apr 2013 09:10:17 -0700 From: Maria Mejia Subject: [Histonet] HM 355S microtome users To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Is anyone currently using the HM 355S full automatic rotary microtome? Please can anyone give me a review of this particular microtome - pros & cons would be very helpful from those individuals actually using this machine on a daily basis. My lab is considering purchasing this microtome. I would be most grateful for any assistance users can provide. Best Maria Mejia Affymetrix, Inc ------------------------------ Message: 21 Date: Thu, 11 Apr 2013 16:43:05 +0000 From: Anatoli Gleiberman Subject: [Histonet] RE: Anti-human nuclear antibody To: Elizabeth Cameron , "histonet@lists.utsouthwestern.edu" Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C016DC952F3@cbiolabs05.CBiolabs.local> Content-Type: text/plain; charset="us-ascii" Elizabeth, We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03), Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 10 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From talulahgosh <@t> gmail.com Thu Apr 11 14:58:21 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Apr 11 14:58:27 2013 Subject: [Histonet] PAP Pen With Aqueous Mounting Media In-Reply-To: <84B03145D9971342951CDF0E003F8874165860@isvifmbxsvr02.meei.harvard.edu> References: <84B03145D9971342951CDF0E003F8874165860@isvifmbxsvr02.meei.harvard.edu> Message-ID: We just leave the pap pen on. Since it's not covering the tissue, it won't matter. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Apr 11, 2013 at 3:29 PM, Langsdorf, Aliete < Aliete_Langsdorf@meei.harvard.edu> wrote: > Hello All, > I would like to use a PAP pen to separate sections on my slide, but have > been using an aqueous (non-xylenes based) mounting media. How do you remove > the PAP pen without using xylenes? Is this done? > Thank you, > ~Ally > > > > Research Technologist > Comander Lab - Ocular Genomics Institute > Massachusetts Eye & Ear Infirmary > Lab: (617)-573-6485 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From modz9636 <@t> gmail.com Thu Apr 11 19:02:22 2013 From: modz9636 <@t> gmail.com (M.O.) Date: Thu Apr 11 19:02:29 2013 Subject: [Histonet] Countertop fume hoods Message-ID: Hello Histonet! I am looking into small countertop fume hoods or maybe a filter system that I can use to place tissue under that has been in formalin or decalcifier to diminish the fumes. Do you have any recommendation on what and where to look for something like this? Thank you! - Merissa From marktarango <@t> gmail.com Thu Apr 11 19:31:18 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Apr 11 19:31:24 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> Message-ID: Can I ask how you test before dumping? Thanks Mark On Apr 11, 2013 6:21 AM, "Cristi Rigazio" wrote: > We neutralize ours and have no problems with it. I am not sure how much > you use, so I will say it is easiest in smaller batches as you do have to > shake it up to make sure it dissolves. We purchase ours from BBC > Biochemical for a very reasonable price and then test before dumping. We > have doing this for four years with no issues. > Thanks, > Cristi > > Sent from my iPhone > > On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" > wrote: > > > We are looking into the option of neutralizing our formalin waste > instead of having it to be picked up. > > If you use Formalin Neutralizer, do you have any pro/con about this > product? > > Thank you very much. > > > > Lin S. Bustamante, B.S., H.T.(ASCP) > > VIBS Histology Laboratory Supervisor > > College Of Veterinary Medicine > > Texas A&M University > > College Station, Texas 77843-4458 > > Phone: (979) 845-3177 > > Fax: (979) 458-3499 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From madelinegi <@t> yahoo.com Thu Apr 11 19:36:54 2013 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Thu Apr 11 19:36:56 2013 Subject: [Histonet] Blocks and slide labeling Message-ID: <1365727014.28885.YahooMailClassic@web163906.mail.gq1.yahoo.com> Hello everyone I have a question about labeling slides and blocks, I run a POL GI lab I was just certified by CAP and I wanted to understand if it is necessary to write GAR13-0123 on both the slides and blocks. We write the patients last name on both, but writing the? GAR and an extra 0 is becoming an issue when trying to read the blocks after processing could i write it as G13-123 with the patients last name of course, could I get?some advice please?Thank you. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From cls71877 <@t> gmail.com Thu Apr 11 20:26:50 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Thu Apr 11 20:27:00 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> Message-ID: <75E89B5D-FC9D-44C5-B84E-CEFB05C4F1AB@gmail.com> We have a formaldehyde test kit. It's a dip stick type test. Sent from my iPhone On Apr 11, 2013, at 5:31 PM, Mark Tarango wrote: > Can I ask how you test before dumping? > > Thanks > > Mark > > On Apr 11, 2013 6:21 AM, "Cristi Rigazio" wrote: >> We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. >> Thanks, >> Cristi >> >> Sent from my iPhone >> >> On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" wrote: >> >> > We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. >> > If you use Formalin Neutralizer, do you have any pro/con about this product? >> > Thank you very much. >> > >> > Lin S. Bustamante, B.S., H.T.(ASCP) >> > VIBS Histology Laboratory Supervisor >> > College Of Veterinary Medicine >> > Texas A&M University >> > College Station, Texas 77843-4458 >> > Phone: (979) 845-3177 >> > Fax: (979) 458-3499 >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CCochran <@t> ameripath.com Thu Apr 11 23:09:19 2013 From: CCochran <@t> ameripath.com (Cochran, Cynthia S) Date: Thu Apr 11 23:09:28 2013 Subject: [Histonet] - Referrals for Open Position Message-ID: <75903DEC8F3B7E4D9B9543CA71A8859803195AD4F2@MWNMAIL00.ameripath.local> Hello Histonet! I am actively searching for a night shift Histotech with experience to work in my Shelton, CT lab. Please feel free to reach out to me via email for more details or pass this information onto your contacts. Thank you and I hope everyone is well! Cyndi Cochran Sr. Staffing Specialist From Sandra.Harrison3 <@t> va.gov Fri Apr 12 08:33:43 2013 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Apr 12 08:34:42 2013 Subject: [Histonet] Countertop fume hoods In-Reply-To: References: Message-ID: Dear Merissa, I have used the Labconco countertop fume hood in a few different Histology settings, both MOHS and general, and found it to be very compact, easy to change the filter, and very effective at pulling the fumes away from the user. It is also a ductless system. See http://www.labconco.com/product/fume-adsorbers/35 Sincerely, Sandy Harrison VA Histology Supervisor, Minneapolis, MN 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of M.O. Sent: Thursday, April 11, 2013 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Countertop fume hoods Hello Histonet! I am looking into small countertop fume hoods or maybe a filter system that I can use to place tissue under that has been in formalin or decalcifier to diminish the fumes. Do you have any recommendation on what and where to look for something like this? Thank you! - Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mhorne <@t> upei.ca Fri Apr 12 08:59:33 2013 From: Mhorne <@t> upei.ca (Margaret Horne) Date: Fri Apr 12 08:59:42 2013 Subject: [Histonet] centrifuge Message-ID: <5167E915020000D100029DBE@oes-grpwise.novell.upei.ca> Hello, I am posting this for a group of veterinary students that volunteer for the local Greyhound Rescue chapter. Just a faint hope. They are looking for a donated centrifuge (benchtop, 5 ml tubes). It is to test the blood samples of rescued dogs to check for parasites before shipping the dogs across provincial/state/national borders. This use would not be restricted to Greyhounds, but also for any other volunteer work that the many veterinary student groups do here; , treating/spay/neuter feral cats, going up North and treating sled dogs, Wild Life rescue etc. We have very active caring students here. They have canvassed the vet college here , university campus etc. but no one has a small unused centrifuge. Thanks, Margaret From BZIMMERM <@t> gru.edu Fri Apr 12 09:39:31 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Apr 12 09:39:36 2013 Subject: [Histonet] GSH update Message-ID: HT review is in session now. The students are very fortunate to have Robert Lott as an instructor. I have to confess I had about a dozen raw oysters last night at SeaJays but did cold coke chasers to kill the germs. We sat outside on the veranda and the weather was perfect. Vendors are setting up and we are looking forward to the vendor reception tonight. Sent from my iPhone Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From ratliffjack <@t> hotmail.com Fri Apr 12 09:54:55 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Apr 12 09:55:05 2013 Subject: [Histonet] GSH update In-Reply-To: References: Message-ID: If you're a local Histotech or Pathologist within a 2 1/2 hour drive of Jekyll Island, you still have time to catch the second workshop of the day (1:30pm EST)....Laser Microtomy: The Future of Soft & Hard Tissue Histology! You won't want to miss this introduction to the use of femtosecond lasers to section fresh and resin embedded tissues! Jack On Apr 12, 2013, at 10:39 AM, "Zimmerman, Billie" wrote: > HT review is in session now. The students are very fortunate to have Robert Lott as an instructor. I have to confess I had about a dozen raw oysters last night at SeaJays but did cold coke chasers to kill the germs. We sat outside on the veranda and the weather was perfect. > Vendors are setting up and we are looking forward to the vendor reception tonight. > Sent from my iPhone > Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMitchell <@t> uwhealth.org Fri Apr 12 13:20:49 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Fri Apr 12 13:20:59 2013 Subject: [Histonet] Thioflavine S for Amyloid Message-ID: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 From rjbuesa <@t> yahoo.com Fri Apr 12 13:32:49 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 12 13:32:53 2013 Subject: [Histonet] Thioflavine S for Amyloid In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> References: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: <1365791569.41892.YahooMailNeo@web163102.mail.bf1.yahoo.com> It is faster but ephemeral. Fluorescence is what is seen. Ren? J. From: Mitchell Jean A To: "histonet@lists.utsouthwestern.edu" Sent: Friday, April 12, 2013 2:20 PM Subject: [Histonet] Thioflavine S for Amyloid Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening.? Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI? 53792-5132 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Apr 12 13:53:45 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Apr 12 13:53:52 2013 Subject: [Histonet] RE: Thioflavine S for Amyloid In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> References: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: Jean, obvious disadvantage is you need a fluorescence microscope, but then you need polarizing filters for Congo red (not as expensive though!) I haven't used Thioflavine S but Thioflavine T. My understanding is that Thioflavine S, which is a chemically different dye, tends to fluoresce strongly but nonselectively, and can overstain the whole section. Either way, the staining is not light-fast! Sections need to be kept in the dark until examined and usually within no more than a couple of days. I used it a lot back home where it seemed to be preferred more than Congo red. It is a very simple stain to perform, and obviously can be screened quickly. Dissolving the dye in 0.1M HCl gives greater selectivity. The dye soluton has a shelf-life of a month or more if kept in the dark. It is important to counterstain the sections with Mayer's hematoxylin as this quenches any nuclear fluorescence. Elastic fibers, fibrinoid, mast cell granules and Paneth cell granules fluoresce with this method but they can be easily identified as such and ignored The method of Burns et al gives probably the most consistent results: Burns J, Pennock CA, Stoward PJ. The specificity of staining of amyloid deposits with Thioflavine T. J Pathol Bacteriol 1967; 94: 337-344 Intersting comments in Puchtler's paper: Puchtler H, Sweat Waldrop F, Meloan S. Application of thiazole dyes to amyloid under conditions of direct cotton dyeing: correlation of histochemical and chemical data. Histochemistry 1983; 77: 431-445 Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Friday, April 12, 2013 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thioflavine S for Amyloid Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Aliete_Langsdorf <@t> MEEI.HARVARD.EDU Fri Apr 12 14:19:13 2013 From: Aliete_Langsdorf <@t> MEEI.HARVARD.EDU (Langsdorf, Aliete) Date: Fri Apr 12 14:19:19 2013 Subject: [Histonet] Re: Anti-human nuclear antibody Message-ID: <84B03145D9971342951CDF0E003F8874171950@isvifmbxsvr01.meei.harvard.edu> You may also want to try the anti-NuMA antibody from cell signaling (#3888) - (rabbit polyclonal). I have found it to be an extremely clean antibody, excellent for detecting even one or two human cells lodged in mouse tissue. Good luck! ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye & Ear Infirmary Lab: (617)-573-6485 From amosbrooks <@t> gmail.com Fri Apr 12 17:02:07 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Apr 12 17:02:13 2013 Subject: [Histonet] Re: Histonet Digest, Vol 113, Issue 10 In-Reply-To: <5166ec2a.0715e00a.5fab.0639SMTPIN_ADDED_MISSING@mx.google.com> References: <5166ec2a.0715e00a.5fab.0639SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Lin, I understand the desire to reduce the amount of waste hauled off as hazardous material. The issue I had with this product is that there was very little information about the product itself in the MSDS. Technically it couldn't be determined if the neutralizer itself could be dumped down the drain, much less in the company of formalin. The next question of weather the formalin was fully neutralized or not. There isn't really a good way to prove the formalin has actually been neutralized short of testing the waste solution with tests that are likely more expensive than the waste hauler in the first place. Ultimately, the decision should be made by your Department of Environmental Protection. It would be best to share the MSDS & directions for use with them and let them decide if it is a good idea before trying to use it. Amos Brooks On Thu, Apr 11, 2013 at 1:00 PM, wrote: > Message: 9 > Date: Thu, 11 Apr 2013 13:04:21 +0000 > From: "Bustamante, Lin" > Subject: [Histonet] Formalin Neutralizer > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> > Content-Type: text/plain; charset="us-ascii" > > We are looking into the option of neutralizing our formalin waste instead > of having it to be picked up. > If you use Formalin Neutralizer, do you have any pro/con about this > product? > Thank you very much. > > Lin S. Bustamante, B.S., H.T.(ASCP) > From marktarango <@t> gmail.com Fri Apr 12 17:22:02 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 12 17:22:07 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: <75E89B5D-FC9D-44C5-B84E-CEFB05C4F1AB@gmail.com> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> <75E89B5D-FC9D-44C5-B84E-CEFB05C4F1AB@gmail.com> Message-ID: Our safety person claims no test for formalin is accurate after the addition of sodium sulfite. I had suggested some kind of testing after I was surprised by the strength of the fumes when someone was pouring the treated formalin waste down the drain. I wish I knew more about it. On Thu, Apr 11, 2013 at 6:26 PM, Cristi Rigazio wrote: > We have a formaldehyde test kit. It's a dip stick type test. > > Sent from my iPhone > > On Apr 11, 2013, at 5:31 PM, Mark Tarango wrote: > > Can I ask how you test before dumping? > > Thanks > > Mark > On Apr 11, 2013 6:21 AM, "Cristi Rigazio" wrote: > >> We neutralize ours and have no problems with it. I am not sure how much >> you use, so I will say it is easiest in smaller batches as you do have to >> shake it up to make sure it dissolves. We purchase ours from BBC >> Biochemical for a very reasonable price and then test before dumping. We >> have doing this for four years with no issues. >> Thanks, >> Cristi >> >> Sent from my iPhone >> >> On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" >> wrote: >> >> > We are looking into the option of neutralizing our formalin waste >> instead of having it to be picked up. >> > If you use Formalin Neutralizer, do you have any pro/con about this >> product? >> > Thank you very much. >> > >> > Lin S. Bustamante, B.S., H.T.(ASCP) >> > VIBS Histology Laboratory Supervisor >> > College Of Veterinary Medicine >> > Texas A&M University >> > College Station, Texas 77843-4458 >> > Phone: (979) 845-3177 >> > Fax: (979) 458-3499 >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From akemiat3377 <@t> yahoo.com Fri Apr 12 23:26:53 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Apr 12 23:27:01 2013 Subject: [Histonet] Share room for NSH Message-ID: <1365827213.38092.YahooMailNeo@web140601.mail.bf1.yahoo.com> Regarding NSH 40th Anniversary Meeting: I'm trying to cut costs to attend. ?Are any of my friends going to the NSH meeting that would like to share a room? ?If you are giving a workshop, I would be more than happy to compensate you with some sort of a stipend since you get your room paid for. ?Let me know. ?I am looking not only for workshop presenters. It would be nice to share a room with a person I know, but am willing to get to know new friends. ?It helps to reduce costs and get to go! Thanks, Akemi ? Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From rjbuesa <@t> yahoo.com Sat Apr 13 10:37:29 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 13 10:37:39 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> <75E89B5D-FC9D-44C5-B84E-CEFB05C4F1AB@gmail.com> Message-ID: <1365867449.57624.YahooMailNeo@web163103.mail.bf1.yahoo.com> The topic of formalin neutralization comes up regularly in HistoNet and there are always those who post answers telling about good results obtained and the advantage of eliminating the cost of removal by some local company dedicated to chemical contaminants removal. Perhaps I have been unlucky but?I have tried Transform121; Vytak; Formalex; Aldez-AMS 410 and 1% aq. potassium permanganate with very poor results as tested with Schiff's reagent of the "neutralized" product. Always?the neutralized NBF had?a strong?smell of?formalin, as Mark describes. On an answer to HistoNet on March 24, 2009 our colleague Tony Henwwod advised all of us that 10% neutral formalin can be neutralized with sodium bisulfite or with concentrated ammonia (27%) used at a ratio of 56mL every 1 000 mL of 10% NBF. The ammonia is added stirring and is an exothermic chemical reaction. I have not tested the procedure but I completely sure of?Tony's advise. After my failures with commercial neutralizers I decided to keep using the services of the chemical disposal company, expensive as they were, because I could reconcile myself with the idea of dumping incompletely "neutralized" NBF into the sewer and that is what I recommend anybody who, after "neutralizing" the NBF, still smells it! Ren? J.? From: Mark Tarango To: Cristi Rigazio Cc: "histonet@lists.utsouthwestern.edu" ; Lin Bustamante Sent: Friday, April 12, 2013 6:22 PM Subject: Re: [Histonet] Formalin Neutralizer Our safety person claims no test for formalin is accurate after the addition of sodium sulfite.? I had suggested some kind of testing after I was surprised by the strength of the fumes when someone was pouring the treated formalin waste down the drain.? I wish I knew more about it. On Thu, Apr 11, 2013 at 6:26 PM, Cristi Rigazio wrote: > We have a formaldehyde test kit.? It's a dip stick type test. > > Sent from my iPhone > > On Apr 11, 2013, at 5:31 PM, Mark Tarango wrote: > > Can I ask how you test before dumping? > > Thanks > > Mark > On Apr 11, 2013 6:21 AM, "Cristi Rigazio" wrote: > >> We neutralize ours and have no problems with it.? I am not sure how much >> you use, so I will say it is easiest in smaller batches as you do have to >> shake it up to make sure it dissolves.? We purchase ours from BBC >> Biochemical for a very reasonable price and then test before dumping.? We >> have doing this for four years with no issues. >> Thanks, >> Cristi >> >> Sent from my iPhone >> >> On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" >> wrote: >> >> > We are looking into the option of neutralizing our formalin waste >> instead of having it to be picked up. >> > If you use Formalin Neutralizer, do you have any? pro/con about this >> product? >> > Thank you very much. >> > >> > Lin S. Bustamante, B.S., H.T.(ASCP) >> > VIBS Histology Laboratory Supervisor >> > College Of Veterinary Medicine >> > Texas A&M University >> > College Station, Texas 77843-4458 >> > Phone: (979) 845-3177 >> > Fax: (979) 458-3499 >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benjamin <@t> histologistics.com Sat Apr 13 11:16:47 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Sat Apr 13 11:17:02 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: <1365867449.57624.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> <75E89B5D-FC9D-44C5-B84E-CEFB05C4F1AB@gmail.com> <1365867449.57624.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: Lol, of all your post Rene, I have not seen anything that needed more. Your a good guy. Always detailed and precise, with a complete story not just bare minimum effort. In this case I have to point something out, not so much for histonet as for Rene to thank him for all his histonet education he gives us all. So here goes--- formaldehyde is a colorless odorless gas. You cannot smell it. The smell of formalin is an additive, the purpose of which is to make you aware when you are being exposed. Propane has a similar additive that smells like rotten eggs, so you dont blow your house or garage up leaving the stove or grill on all day and then lighting it up! Boom! To elaborate on the formalin neutralizing blah blah- you make hexamethylene tetramine by mixing ammonia and formaldehyde...its a common compound used in platics and other industry and also to treat UTI. Since it is a drug, it is allready found in our water system. Just pour that crap down the drain Rene! Dont worry about the smell its harmless! And keep posting on histonet! Sent from my iPhone On Apr 13, 2013, at 11:37 AM, Rene J Buesa wrote: > The topic of formalin neutralization comes up regularly in HistoNet and there are always those who post answers telling about good results obtained and the advantage of eliminating the cost of removal by some local company dedicated to chemical contaminants removal. > Perhaps I have been unlucky but I have tried Transform121; Vytak; Formalex; Aldez-AMS 410 and 1% aq. potassium permanganate with very poor results as tested with Schiff's reagent of the "neutralized" product. Always the neutralized NBF had a strong smell of formalin, as Mark describes. > On an answer to HistoNet on March 24, 2009 our colleague Tony Henwwod advised all of us that 10% neutral formalin can be neutralized with sodium bisulfite or with concentrated ammonia (27%) used at a ratio of 56mL every 1 000 mL of 10% NBF. The ammonia is added stirring and is an exothermic chemical reaction. > I have not tested the procedure but I completely sure of Tony's advise. > After my failures with commercial neutralizers I decided to keep using the services of the chemical disposal company, expensive as they were, because I could reconcile myself with the idea of dumping incompletely "neutralized" NBF into the sewer and that is what I recommend anybody who, after "neutralizing" the NBF, still smells it! > Ren? J. > > From: Mark Tarango > To: Cristi Rigazio > Cc: "histonet@lists.utsouthwestern.edu" ; Lin Bustamante > Sent: Friday, April 12, 2013 6:22 PM > Subject: Re: [Histonet] Formalin Neutralizer > > > Our safety person claims no test for formalin is accurate after the > addition of sodium sulfite. I had suggested some kind of testing after I > was surprised by the strength of the fumes when someone was pouring the > treated formalin waste down the drain. I wish I knew more about it. > > > > > On Thu, Apr 11, 2013 at 6:26 PM, Cristi Rigazio wrote: > >> We have a formaldehyde test kit. It's a dip stick type test. >> >> Sent from my iPhone >> >> On Apr 11, 2013, at 5:31 PM, Mark Tarango wrote: >> >> Can I ask how you test before dumping? >> >> Thanks >> >> Mark >> On Apr 11, 2013 6:21 AM, "Cristi Rigazio" wrote: >> >>> We neutralize ours and have no problems with it. I am not sure how much >>> you use, so I will say it is easiest in smaller batches as you do have to >>> shake it up to make sure it dissolves. We purchase ours from BBC >>> Biochemical for a very reasonable price and then test before dumping. We >>> have doing this for four years with no issues. >>> Thanks, >>> Cristi >>> >>> Sent from my iPhone >>> >>> On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" >>> wrote: >>> >>>> We are looking into the option of neutralizing our formalin waste >>> instead of having it to be picked up. >>>> If you use Formalin Neutralizer, do you have any pro/con about this >>> product? >>>> Thank you very much. >>>> >>>> Lin S. Bustamante, B.S., H.T.(ASCP) >>>> VIBS Histology Laboratory Supervisor >>>> College Of Veterinary Medicine >>>> Texas A&M University >>>> College Station, Texas 77843-4458 >>>> Phone: (979) 845-3177 >>>> Fax: (979) 458-3499 >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Apr 13 12:52:47 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 13 12:52:55 2013 Subject: [Histonet] Formalin Neutralizer In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9@CVMMB02.cvm.tamu.edu> <1F23ED0A-515F-4C68-8FC7-505BBF40F22C@gmail.com> <75E89B5D-FC9D-44C5-B84E-CEFB05C4F1AB@gmail.com> <1365867449.57624.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <1365875567.70605.YahooMailNeo@web163103.mail.bf1.yahoo.com> Benjamin: Thank you???????? Just a "detail" about formaldehyde (chemically named methanal). Please check ANY chemical book and you will find in the description of methanal something like: "colorless gas at room temperature with a CHARACTERISTIC PUNGENT and?IRRITATING ODOR"!! So much for "you cannot smell it!!! And be assured I will keep posting every time I think I can be of some help to my fellow "histonetters" Ren? J. From: Benjamin To: Rene J Buesa Cc: Mark Tarango ; Cristi Rigazio ; "histonet@lists.utsouthwestern.edu" ; Lin Bustamante Sent: Saturday, April 13, 2013 12:16 PM Subject: Re: [Histonet] Formalin Neutralizer Lol, of all your post Rene, I have not seen anything that needed more. Your a good guy. Always detailed and precise, with a complete story not just bare minimum effort. In this case I have to point something out, not so much for histonet as for Rene to thank him for all his histonet education he gives us all. So here goes--- formaldehyde is a colorless odorless gas. You cannot smell it. The smell of formalin is an additive, the purpose of which is to make you aware when you are being exposed. Propane has a similar additive that smells like rotten eggs, so you dont blow your house or garage up leaving the stove or grill on all day and then lighting it up! Boom! To elaborate on the formalin neutralizing blah blah- you make?hexamethylene tetramine by mixing ammonia and formaldehyde...its a common compound used in platics and other industry and also to treat UTI. Since it is a drug, it is allready found in our water system. Just pour that crap down the drain Rene! Dont worry about the smell its harmless! And keep posting on histonet! Sent from my iPhone On Apr 13, 2013, at 11:37 AM, Rene J Buesa wrote: The topic of formalin neutralization comes up regularly in HistoNet and there are always those who post answers telling about good results obtained and the advantage of eliminating the cost of removal by some local company dedicated to chemical contaminants removal. >Perhaps I have been unlucky but?I have tried Transform121; Vytak; Formalex; Aldez-AMS 410 and 1% aq. potassium permanganate with very poor results as tested with Schiff's reagent of the "neutralized" product. Always?the neutralized NBF had?a strong?smell of?formalin, as Mark describes. >On an answer to HistoNet on March 24, 2009 our colleague Tony Henwwod advised all of us that 10% neutral formalin can be neutralized with sodium bisulfite or with concentrated ammonia (27%) used at a ratio of 56mL every 1 000 mL of 10% NBF. The ammonia is added stirring and is an exothermic chemical reaction. >I have not tested the procedure but I completely sure of?Tony's advise. >After my failures with commercial neutralizers I decided to keep using the services of the chemical disposal company, expensive as they were, because I could reconcile myself with the idea of dumping incompletely "neutralized" NBF into the sewer and that is what I recommend anybody who, after "neutralizing" the NBF, still smells it! >Ren? J.? > >From: Mark Tarango >To: Cristi Rigazio >Cc: "histonet@lists.utsouthwestern.edu" ; Lin Bustamante >Sent: Friday, April 12, 2013 6:22 PM >Subject: Re: [Histonet] Formalin Neutralizer > > >Our safety person claims no test for formalin is accurate after the >addition of sodium sulfite.? I had suggested some kind of testing after I >was surprised by the strength of the fumes when someone was pouring the >treated formalin waste down the drain.? I wish I knew more about it. > > > > >On Thu, Apr 11, 2013 at 6:26 PM, Cristi Rigazio wrote: > > >We have a formaldehyde test kit.? It's a dip stick type test. >> > >> >Sent from my iPhone >> > >> >On Apr 11, 2013, at 5:31 PM, Mark Tarango wrote: >> > >> >Can I ask how you test before dumping? >> > >> >Thanks >> > >> >Mark >> >On Apr 11, 2013 6:21 AM, "Cristi Rigazio" wrote: >> > >> >We neutralize ours and have no problems with it.? I am not sure how much >>> >you use, so I will say it is easiest in smaller batches as you do have to >>> >shake it up to make sure it dissolves.? We purchase ours from BBC >>> >Biochemical for a very reasonable price and then test before dumping.? We >>> >have doing this for four years with no issues. >>> >Thanks, >>> >Cristi >>> > >>> >Sent from my iPhone >>> > >>> >On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" >>> >wrote: >>> > >>> >We are looking into the option of neutralizing our formalin waste >>>> >instead of having it to be picked up. >>> >If you use Formalin Neutralizer, do you have any? pro/con about this >>>> >product? >>> >Thank you very much. >>>> > >>>> >Lin S. Bustamante, B.S., H.T.(ASCP) >>>> >VIBS Histology Laboratory Supervisor >>>> >College Of Veterinary Medicine >>>> >Texas A&M University >>>> >College Station, Texas 77843-4458 >>>> >Phone: (979) 845-3177 >>>> >Fax: (979) 458-3499 >>>> > >>>> >_______________________________________________ >>>> >Histonet mailing list >>>> >Histonet@lists.utsouthwestern.edu >>>> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> > >>> >_______________________________________________ >>> >Histonet mailing list >>> >Histonet@lists.utsouthwestern.edu >>> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> > >>_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Sat Apr 13 13:01:48 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Apr 13 13:01:52 2013 Subject: [Histonet] Re: Thioflavine Staining for Amyloid Message-ID: Thioflavine T enjoyed a fairly brief vogue as an amyloid stain about the time I was a pathology resident over forty of years ago. It awed the professors of medicine because it was a fluorescent stain back when that was a hot topic. I understood that it was similar in specificity to the old crystal violet mounted in pancake syrup. The standard stain for amyloid remains Congo red, viewed with polarization. Newer direct cotton dyes like Sirius red never really caught on. Anatech (www.anatechltdusa.com - I have no connection with Anatech) introduced "Amyloid Red" (Direct red 72, C.I. 29200) as an amyloid stain twelve years ago. I've yet to read about it anywhere - a Google search turns up nothing but their catalog and a Histosearch note I wrote in 2001. Does anyone have any experience with it? Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Sat Apr 13 13:09:35 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Apr 13 13:09:38 2013 Subject: [Histonet] Re: Formalin Neutralizer Message-ID: I'm surprised that the dismal topic of formaldehyde neutralization never seems to get settled. I have a litre of 10% neutral buffered formalin. How many grams of sodium sulfite (or bisulfite, or metabisulfite) do I have to weigh out and pour in to neutralize it? What is the chemical reaction? Is ammonia also required? and if so, I've got the same questions about ammonia. Will the Herrn Inschpektors allow me to use generic chemicals, or do I have to buy some expensive proprietary product in order to satisfy them? Bob Richmond Samurai Pathologist Maryville TN From benjamin <@t> histologistics.com Sat Apr 13 17:04:08 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Sat Apr 13 17:04:21 2013 Subject: [Histonet] Re: Formalin Neutralizer In-Reply-To: References: Message-ID: <1304E2A8-8DAE-48BE-B918-579F9F165220@histologistics.com> I am posting that my last comment about formalin was wrong about the odor, it does not have any additives. And as far as dumping it down the drain follow your local regs!! Sent from my iPhone On Apr 13, 2013, at 2:09 PM, Bob Richmond wrote: > I'm surprised that the dismal topic of formaldehyde neutralization never > seems to get settled. > > I have a litre of 10% neutral buffered formalin. How many grams of sodium > sulfite (or bisulfite, or metabisulfite) do I have to weigh out and pour in > to neutralize it? What is the chemical reaction? Is ammonia also required? > and if so, I've got the same questions about ammonia. > > Will the Herrn Inschpektors allow me to use generic chemicals, or do I have > to buy some expensive proprietary product in order to satisfy them? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Apr 14 07:25:19 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Apr 14 07:25:23 2013 Subject: [Histonet] Thioflavine S for Amyloid In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> References: <16F90B93CA23D446980B3D591FD02DAD051B3B@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: Congo red is the gold standard for amyloid staining. It is the most sensitive of the amyloid stain, at about 97%. However, sometimes the Congo red will not stain the amyloid protein, such as when the amyloid is a large, very old deposit. In that case, more and more amyloid is being crammed into the same space, and the beta pleats become warped. For Congo red staining to work, the beta pleats must be consistently at a certain distance apart (7 um apart, if I remember correctly). Congo red is a linear dye, with 2 SO3- groups, one at each end. So it binds to the amyloid protein pleats, one dye right after another | | | | |, and that's why it will polarize. But if the beta pleats are not lining up | \ \ | \ / / | | \ /, then the Congo red, may not be able to bind, as the binding sites on the amyloid are not the correct distance apart. And it the Congo red can bind, it is not lining up | | | | |, but is binding in all directions, similar to the warped beta pleats. Therefore it will not birefringe/polarize. Overfixation in formalin will do the same thing, as there will be too many formalin cross-links, warping the beta pleats. Therefore, when it is suspected that the person really has amyloid, but the Congo red isn't working (remember, it's 97% sensitive, which means it's not demonstrating amyloid 3% of the time), it's good to have back ups, which we have used, and have been able to demonstrate amyloid when the Congo red doesn't work. Since the alternatives aren't as specific or sensitive for amyloid as Congo red, when we have to go to our backups, we tend to do all three, on the theory that even though they aren't as sensitive or specific, since they are all staining a different aspect of amyloid, and if all three are showing positivity, then it most be amyloid. Congo red, viewed with fluoresence microscope. Use the auramine-rhodamine AFB filters (hit it with 540 green light), and the Congo red-amyloid will fluoresce orange. Crystal violet or Methyl violet - polychromatic dyes that bind to carboxyl ions on amyloid. So one dye component stains the amyloid a violet color, while the other dye components stain the background a blue-purple. Need to use aqueous mounting media, so not a permanent stain. Not as sensitive (about 70%) or specific as Congo red. Will stain mucin. AL Amyloid also tends to have a lower concentration of surface carboxyl ions, so tends to have negative staining with the CV or MV stains. Thioflavin T (TFT) and Thioflaving S (TFS) are fluorochromes, so need a fluorescence miscroscope, blue light excitation at 490, similar to FITC, and these dyes will fluoresce yellow. These dyes appear to stain the P component on amyloid, which is a pentagonal shaped polysaccharide protein, which is a normal protein in our blood (alpha-globin), but for some reason attaches itself to amyloid. It's a fast stain, easy to do, very sensitive for amyloid. However, you do need a FITC fluoresence microscope, and since it's an aqueous mount, it's not permanent. It's not specific for amyloid, as other components will be stained and fluoresce yellow, or will autofluoresce yellow (such as elastin fibers, dense connective tissue, lipofuchsin, a lot of other granules). I've only used TFT, so I can't say if TFS is any better. So TFS is good for a back up, but I would continue with Congo red, or the Amyloid red from Anatech, to be the primary amyloid stain. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 Opinions expressed are mine, and do not reflect on my place of employment. -----Original Message----- From: Mitchell Jean A Sent: Friday, April 12, 2013 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thioflavine S for Amyloid Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Apr 14 09:19:04 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 14 09:19:12 2013 Subject: [Histonet] Re: Formalin Neutralizer In-Reply-To: <1304E2A8-8DAE-48BE-B918-579F9F165220@histologistics.com> References: <1304E2A8-8DAE-48BE-B918-579F9F165220@histologistics.com> Message-ID: <1365949144.46423.YahooMailNeo@web163104.mail.bf1.yahoo.com> Benjamin: You are wrong again because "formalin" is the commercial brand name used to define the about 47% aq. solution of methanal in dist.?water and, as a matter of fact, it does contain an additive (methanol) to prevent its polymerization into para-formaldehyde. Ren? J. From: Benjamin To: Bob Richmond Cc: "Histonet@lists.utsouthwestern.edu" Sent: Saturday, April 13, 2013 6:04 PM Subject: Re: [Histonet] Re: Formalin Neutralizer I am posting that my last comment about formalin was wrong about the odor, it does not have any additives. And as far as dumping it down the drain follow your local regs!! Sent from my iPhone On Apr 13, 2013, at 2:09 PM, Bob Richmond wrote: > I'm surprised that the dismal topic of formaldehyde neutralization never > seems to get settled. > > I have a litre of 10% neutral buffered formalin. How many grams of sodium > sulfite (or bisulfite, or metabisulfite) do I have to weigh out and pour in > to neutralize it? What is the chemical reaction? Is ammonia also required? > and if so, I've got the same questions about ammonia. > > Will the Herrn Inschpektors allow me to use generic chemicals, or do I have > to buy some expensive proprietary product in order to satisfy them? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chesarato <@t> hotmail.com Sun Apr 14 15:51:34 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sun Apr 14 15:51:39 2013 Subject: [Histonet] Amyloid Message-ID: I think this is a great article to read about amyloid. This is the link to the paper http://propath.com/newsletters-immunohistochemistry-173/181-september-2004-immunohistochemistry-in-amyloidosis It belongs to a great Pathologist Dr. Rodney T. Miller Dr. C?sar RomeroBuenos AiresArgentina From JMacDonald <@t> mtsac.edu Sun Apr 14 18:44:52 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Apr 14 18:44:58 2013 Subject: [Histonet] California Society For Histotechnology Meeting Message-ID: The 37th Annual California Society for Histotechnology Meeting is coming up. We will be meeting in Burlingame (San Francisco) May 3-5. Lots of great workshops. For more information follow the link: http://www.californiahistology.org/events.html From JMacDonald <@t> mtsac.edu Sun Apr 14 20:02:24 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Apr 14 20:02:31 2013 Subject: [Histonet] RE: California Society for Histotechnology Meeting May 3-5 In-Reply-To: References: Message-ID: Can the room block rate be extended? From: James Watson To: James Watson , "'Histonet@lists.utsouthwestern.edu'" Date: 04/10/2013 07:35 AM Subject: [Histonet] RE: California Society for Histotechnology Meeting May 3-5 Sent by: histonet-bounces@lists.utsouthwestern.edu I was just informed that On Line registration is now working through our website. http://www.californiahistology.org/ I have also been told this can be accessed by smartphone, I-pad..... also payment can be made through PayPal or Credit Card. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, April 09, 2013 7:25 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] California Society for Histotechnology Meeting May 3-5 Joining the other Societies advertising their meeting on the Histonet. Our meeting this year is in Burlingame near the San Francisco international airport. Our annual symposium is about a month away. Hope that you are planning on joining us in Burlingame. Our new website is still having problems with the online registration, our webmaster is working hard to get it going. But we are not sure when it will be available, so you should consider filling out the attached registration from and mailing it to us. We already have 30 Vendors signed up to attend and have outstanding speakers coming from all over the country with a wide variety of backgrounds and subjects. We are hoping to have another great meeting. See you there. Visit our website at to see the program and mail in registration form. http://www.californiahistology.org/ James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lins0701 <@t> gmail.com Sun Apr 14 20:47:40 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Sun Apr 14 20:47:43 2013 Subject: [Histonet] special stain reagents change Message-ID: Coming from a large reference laboratory, it was crucial to change out reagents for special stains every week since our workload was so heavy. Now at a smaller GI lab, I am wondering if it is absolutely necessary to change out the reagents so frequently? We have changed about 2-3 weeks depending on the amount of slide volume (the kits say up to 300-500 slides- AMT Kits). We were changing every week in the beginning, but one week our reagents were delayed so we changed it out 2 weeks later, and have noticed that the reagents still are "fresh" and the staining quality is not compromised. The alcohols, blueing, and xylene is changed every day. Any thoughts? Thanks in advance! Sophia From rjbuesa <@t> yahoo.com Mon Apr 15 07:05:35 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 15 07:05:40 2013 Subject: [Histonet] special stain reagents change In-Reply-To: References: Message-ID: <1366027535.19526.YahooMailNeo@web163101.mail.bf1.yahoo.com> If your stains contains a guideline about how many slides can be stained with them, just follow those guidelines and I am sure you will be fine. Ren? J. From: Sophia Lin To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 14, 2013 9:47 PM Subject: [Histonet] special stain reagents change Coming from a large reference laboratory, it was crucial to change out reagents for special stains every week since our workload was so heavy. Now at a smaller GI lab, I am wondering if it is absolutely necessary to change out the reagents so frequently? We have changed about 2-3 weeks depending on the amount of slide volume (the kits say up to 300-500 slides- AMT Kits). We were changing every week in the beginning, but one week our reagents were delayed so we changed it out 2 weeks later, and have noticed that the reagents still are "fresh" and the staining quality is not compromised.? The alcohols, blueing, and xylene is changed every day. Any thoughts? Thanks in advance! Sophia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Mon Apr 15 08:36:39 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Apr 15 08:36:56 2013 Subject: [Histonet] Placentas and AP Message-ID: <015d01ce39de$45302870$cf907950$@imagesbyhopper.com> I have been asked this set of questions and thought I would ask the experts! This is regarding sending placentas to the lab for AP evaluations: Is there an industry standard, CAP or Joint Commission requirement for sending placentas for AP evaluations? Such as send all for evaluations; send complicated ones only etc. If there are specific requirements, can you please give me the req as well? Thanks for reading and considering, Michelle _____ No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.3272 / Virus Database: 3162/6245 - Release Date: 04/14/13 From craigak12 <@t> gmail.com Mon Apr 15 10:51:06 2013 From: craigak12 <@t> gmail.com (Jb) Date: Mon Apr 15 10:51:16 2013 Subject: [Histonet] HP: Message-ID: <2E048A02-4FE7-45E1-9974-41D2C355F0A2@gmail.com> Is anyone using Thermo Scientific HP concentrate antibody and have a good dilution? Trying to get it up quickly any help would be greatly appreciated. Thank you.. Sent from my iPhone From TGoins <@t> mt.gov Mon Apr 15 11:05:34 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Apr 15 11:05:47 2013 Subject: [Histonet] HP: In-Reply-To: <2E048A02-4FE7-45E1-9974-41D2C355F0A2@gmail.com> References: <2E048A02-4FE7-45E1-9974-41D2C355F0A2@gmail.com> Message-ID: That would only work if all other reagents and equipment you used were identical to those used from your information source. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, April 15, 2013 9:51 AM To: histonet Subject: [Histonet] HP: Is anyone using Thermo Scientific HP concentrate antibody and have a good dilution? Trying to get it up quickly any help would be greatly appreciated. Thank you.. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Apr 15 11:45:32 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Apr 15 11:45:40 2013 Subject: [Histonet] Hot Histology Job Alert - Great Opportunity in Tyler, Texas. A RELIA Exclusive!! Message-ID: <05fb01ce39f8$a5204070$ef60c150$@earthlink.net> Hi Histonetters! How are you? I wanted to drop you a quick line to ask for help because I am currently recruiting for one of my best clients located in Tyler, TX. This is a permanent full time day shift position in a private dermpath lab. They are looking for someone who is ASCP certified. Experienced and entry level techs are welcome to apply. My client is offering a great salary, terrific benefits, relocation assistance and a great group of people to work with. You will also get the opportunity to learn and work in Mohs on a regular basis along with all other areas of the histology lab. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at relia1@earthlink.net or toll free at 866-607-3542. Remember if I hire someone you refer you will receive a referral bonus! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mhale <@t> MiracaLS.com Mon Apr 15 12:39:26 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Mon Apr 15 12:39:30 2013 Subject: [Histonet] Blades Message-ID: <0E828EC51C7CC445A51E53F81B64E8C721A8B5@s-irv-exchmb.PathologyPartners.intranet> I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From hchidgey <@t> earthlink.net Mon Apr 15 12:48:46 2013 From: hchidgey <@t> earthlink.net (Henry Chidgey) Date: Mon Apr 15 12:49:35 2013 Subject: [Histonet] Question regarding appropriate use of histonet Message-ID: <005801ce3a01$7c705d00$75511700$@net> Howdy Folks, As I am sure you can tell we are subscribers to histonet and appreciate the generosity of members in helping solve the occasional problem we encounter. We are in need of finding either an individual or lab in our area to perform mammal root sectioning and slide preparation for the forensic mammal aging business we operate located near Burnet, Texas in the Texas Hill Country. I would like to reach out to your subscriber base to see if anyone is interested in doing some part time work for us or know someone who might be. Please let me know if that would be an appropriate use of the site. Thanks, Henry Chidgey CoFounder See what folks are saying about us Wildlife Analytical Laboratories www.DeerAge.com PO Box 295 1303 CR 118 B Burnet, Texas 78611 (512) 756-1989 (512) 663-9476 Cell Serving Wildlife Stewards, Ranchers & Hunters Worldwide Customer Service and Quality of Analysis are our Priority and Passion Facebook Eagle Soybeans From Valerie.DeMint <@t> phci.org Mon Apr 15 13:09:10 2013 From: Valerie.DeMint <@t> phci.org (DeMint, Valerie S) Date: Mon Apr 15 13:09:41 2013 Subject: [Histonet] Thermohistoscreen Cassettes Message-ID: <657B95ED46A98B4D9F44EFC67EDCAADD07477024@RWDEX3.WHS.phci.org> Is anyone waiting for their backorder of Thermo Histoscreen Cassettes? We no longer use this cassette and I am trying to sell my remaining inventory. I have several colors available. Write if you want more information :-) Valerie DeMint, HT(ASCP) Waukesha Memorial Hospital Waukesha WI This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From ratliffjack <@t> hotmail.com Mon Apr 15 13:14:28 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Apr 15 13:14:33 2013 Subject: [Histonet] Bone, Biomaterials & Medical Device Implants Histology Event In Cambridge, MA - May 4th, 2013 Message-ID: If you are currently working with bone, biomaterials or medical device implants, you won't want to miss this specialty histology event http://www.polysciences.com/Interactive-Histology-Forum-About/185/ sponsored by Polysciences Inc. and supported with six (6) continuing education units (CEU's) by the National Society for Histotechnology! There is still time to pre-register online http://events.r20.constantcontact.com/register/event?llr=gqebcrbab&oeidk=a07e6rs5o33629b055e or onsite May 4th at the Hyatt Regency Cambridge http://www.polysciences.com/Interactive-Histology-Forum-Hotel-Travel/189/. Topics of discussion will include: A Closer Look at Fixation, Decalcification and Processing Techniques for Paraffin Embedded SpecimensPresented by Robert A. Skinner, HTL (ASCP), Director of Laboratory Resources, Center for Orthopaedic Research, University of Arkansas for Medical Sciences Histology Technique Selection and Challenges for Biomaterial and Medical Device Implanted TissuesPresented by Philip Seifert, MS, HTL (ASCP), Principal Scientist, CBSET, Inc. Acrylic Resins: A Practical Approach for Demonstrating Undermineralized Bone, Biomaterials and Medical Device ImplantsPresented by Jack L. Ratliff, BA, Owner, Ratliff Histology Consultants & Chairman, Hard Tissue Committee National Society for Histotechnology The Use Of A Non-Contact Laser To Collect Thin Sections From A Variety of Soft And Hard Histological Tissues, Synthetic Bio-Resorbable Materials, and Tissues Containing Non-Resorbable Medical Device ImplantsPresented by Heiko Richter, Ph.D., Sales & Product Management, Rowiak GmbH Introduction to Routine Staining and Special Staining with Plastic Resin TechniquesPresented by Valantou Grover, HT (ASCP) HTL, PA, Product Line Manager, Business Manager, Histologist at Polysciences, Inc. If you have any questions, please feel free to contact myself or Ashley Gidzinski (Ashley.Gidzinski@polysciences.com). We look forward to seeing you in Cambridge, MA and May the 4th be with you! Best Regards, Jack Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology From freckles9660 <@t> yahoo.com Mon Apr 15 15:04:21 2013 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Mon Apr 15 15:04:28 2013 Subject: [Histonet] (no subject) Message-ID: <1366056261.91439.YahooMailNeo@web121901.mail.ne1.yahoo.com> How do I boost the power of mucicarmine for staining goblet cells? I have opened a new lot/bottle and have used full strength but it is not staining the cells. Thanks, Karla From denise.long <@t> uconn.edu Mon Apr 15 16:18:07 2013 From: denise.long <@t> uconn.edu (Long, Denise) Date: Mon Apr 15 16:18:13 2013 Subject: [Histonet] Glucose Oxidase blocking method Message-ID: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F72A572@MAILD.grove.ad.uconn.edu> Hello, I'm looking for updated information on the Glucose Oxidase blocking method. In particular, what reagents are being used now that Sigma's G5250 is no longer available. Does anyone know of a company that has this as a kit? Thanks, Denise Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 From JMacDonald <@t> mtsac.edu Mon Apr 15 17:12:23 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 15 17:12:27 2013 Subject: [Histonet] Home based slide interpretation Message-ID: Does anyone know of any regulations against a Pathologist reading slides from home? If so would you be able to cite the specific regulation? Thanks, Jennifer From khadijairfan <@t> yahoo.com Tue Apr 16 00:19:39 2013 From: khadijairfan <@t> yahoo.com (khadija irfan) Date: Tue Apr 16 00:19:47 2013 Subject: [Histonet] Immunohistochemistry for Adipose tissue macrophages in humans Message-ID: <1366089579.51528.YahooMailNeo@web122204.mail.ne1.yahoo.com> Hello All ? I am a clinical researcher so my questions may be somewhat naive, but I would be extremely grateful for any help. I want to look at adipose tissue macrophage polarity by immunohistochemistry for activation markers. I have the following questions: 1. How much tissue sample is required. I want to use subcutaneous and omental fat samples. 2. How should I transport and store my samples. How long can I store them? I anticipate that I would be collecting samples over 2-3 months. 3. If I do H&E staining first, can I do immunohistochemistry on the same slide? Can I do successive markers on the same slide? How many markers can I use it for? ? Thanks ? Khadija From rjbuesa <@t> yahoo.com Tue Apr 16 08:38:26 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 16 08:38:30 2013 Subject: [Histonet] Home based slide interpretation In-Reply-To: References: Message-ID: <1366119506.58355.YahooMailNeo@web163104.mail.bf1.yahoo.com> The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. From: Jennifer MacDonald To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avogaro <@t> science.unitn.it Tue Apr 16 09:30:14 2013 From: avogaro <@t> science.unitn.it (Laura Avogaro) Date: Tue Apr 16 09:30:22 2013 Subject: [Histonet] Picrosirius red on cryosections Message-ID: <2828.192.168.178.78.1366122614.squirrel@www.science.unitn.it> Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy Tel: +39 0461 283425 From liz <@t> premierlab.com Tue Apr 16 09:42:25 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 16 09:43:34 2013 Subject: [Histonet] Picrosirius red on cryosections In-Reply-To: <2828.192.168.178.78.1366122614.squirrel@www.science.unitn.it> References: <2828.192.168.178.78.1366122614.squirrel@www.science.unitn.it> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2AE3@SBS2K8.premierlab.local> Laura This type of analysis does not need to be completed on frozen sections it can be performed on routine 10% NBF fixed paraffin sections. There are protocols on line on the website stains file. Here is the link http://stainsfile.info/StainsFile/stain/conektv/puchtler_psr.htm I would let the frozen sections air dry for at least 30 minutes and then post fix in 10% NBF for about 10 minutes and then start this procedure. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laura Avogaro Sent: Tuesday, April 16, 2013 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Picrosirius red on cryosections Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) - Italy Tel: +39 0461 283425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Apr 16 10:32:41 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 16 10:32:45 2013 Subject: [Histonet] RELIA Hot Job Alert 4-16-2013 Exciting Opportunities in NC.VA, GA, CA, CO, IL, TX and NM Message-ID: <0b2801ce3ab7$a2bec300$e83c4900$@earthlink.net> Hi Histonetters, I hope you enjoyed a beautiful Spring weekend. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance and most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent The histology positions that I am most excited about are located in these areas: Charlotte, NC Salem, VA Staunton, VA Atlanta, GA Long Beach, CA Denver, CO Chicago, IL Springfield, IL Tyler, TX Waco, TX Roswell, NM Long Island, NY Syracuse, NY Louisville, KY East of Columbus, OH If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1@earthlink.net or you can always catch me on cell via call or text at 407-353-5070. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From mjdessoye <@t> commonwealthhealth.net Tue Apr 16 10:53:45 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Tue Apr 16 10:54:25 2013 Subject: [Histonet] SP and CGRP antibodies Message-ID: Hello everyone, Is anyone out there using a commercially available antibody for SP (Substance P) and CGRP (Calcitonin gene-related peptide)? If so, where do you obtain it? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From delsuec <@t> gmail.com Tue Apr 16 11:30:26 2013 From: delsuec <@t> gmail.com (Deloris Carter) Date: Tue Apr 16 11:30:30 2013 Subject: [Histonet] GSH Symposium Message-ID: I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party! Deloris Carter From POWELL_SA <@t> mercer.edu Tue Apr 16 11:52:11 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Apr 16 11:52:17 2013 Subject: [Histonet] GSH Symposium In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE25799E77BB2@MERCERMAIL.MercerU.local> GSH would like to thank you for coming. Glad you had a good time and we will let you know when the next meeting is and the dates as soon as we get them. Crash our party anytime. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Tuesday, April 16, 2013 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GSH Symposium I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party! Deloris Carter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Tue Apr 16 12:35:13 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Apr 16 12:35:18 2013 Subject: [Histonet] Isopentane storage-the polling is open Message-ID: Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 From celebrej <@t> HHSC.CA Tue Apr 16 12:39:24 2013 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Tue Apr 16 12:39:59 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: References: Message-ID: <722701352B76E841BFB9EFAF72553CD269E0521A@ipexmailm02.hhsc.ca> We store our open bottle in an explosion proof fridge and unopened at room temp in a flammable storage cabinet Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From patpxs <@t> gmail.com Tue Apr 16 12:44:23 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Apr 16 12:44:26 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: <722701352B76E841BFB9EFAF72553CD269E0521A@ipexmailm02.hhsc.ca> References: <722701352B76E841BFB9EFAF72553CD269E0521A@ipexmailm02.hhsc.ca> Message-ID: Hi Julia, Thanks for the information. Can you tell me why you store it in the refrigerator? Paula Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, Apr 16, 2013 at 1:39 PM, Celebre Julia wrote: > We store our open bottle in an explosion proof fridge and unopened at room > temp in a flammable storage cabinet > > Julia Celebre Sr MLT > Anatomic Pathology > Hamilton General Hospital > 905-527-4322 ext 46179 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Tuesday, April 16, 2013 1:35 PM > To: HistoNet > Subject: [Histonet] Isopentane storage-the polling is open > > Hello HistoNetters, > > I have been having a discussion with my boss about whether or not to store > the isopentane (used for freezing muscle biopsies) in the refrigerator. (An > expensive, explosion proof one that I would have to buy.) > > Her only experience is with the isopentane stored in the refrigerator, my > experiences are with storing it at room temperature. > > What do you all do? Room temp or refrigerated? > > Any and all comments are greatly appreciated. > > Thanks! > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory Duke University Health > System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 > P: 919.684.2091 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This information is directed in confidence solely to the person named > above and may not otherwise be distributed, copied or disclosed. Therefore, > this information should be considered strictly confidential. If you have > received this email in error, please notify the sender immediately via a > return email for further direction. Thank you for your assistance. > -- From JMitchell <@t> uwhealth.org Tue Apr 16 12:46:06 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Tue Apr 16 12:46:10 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: References: Message-ID: <16F90B93CA23D446980B3D591FD02DAD052897@UWHC-MBX14.uwhis.hosp.wisc.edu> We maintain an approximately 1 liter supply of isopentane and store all isopentane (open/unopened) in an explosion proof freezer. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 12:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Apr 16 13:32:12 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Apr 16 13:32:29 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF079621@ex07.net.ucsf.edu> Paula, we keep ours at room temp in a small flam cab near the muscle bench. We keep two gallons on hand. In Dapson & Dapson's book "Hazardous Materials in the Histopathology Laboratory" (Anatech, 2005) they do not make any specific recommendations concerning storage temperature, except to say IF it is kept in the refrigerator/freezer use only an explosion-proof refrigerator/freezer. The flash point is -50C, so a fridge is not significantly different than room temp, unless your lab is often over 30C/85F (the boiling point!). Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 10:35 AM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Tue Apr 16 13:57:02 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Apr 16 13:57:10 2013 Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 16 In-Reply-To: <20130416170612.9358FB8207C@sms1.georgiahealth.edu> References: <20130416170612.9358FB8207C@sms1.georgiahealth.edu> Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF766CEC@EX-MLB-03.ad.georgiahealth.edu> Thanks to Deloris for taking the time to attend our GSH symposium and her crochet bikini was beautiful! Thanks also to Janet Hobbs for bringing her crochet bikini. The speakers as well as the vendors were wonderful. It was a packed house and a lot of fun. Who says learning can't be fun?! It was a Class Act and we're looking forward to next year. Thanks again to everyone that attended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 16, 2013 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Blades (Hale, Meredith) 2. Question regarding appropriate use of histonet (Henry Chidgey) 3. Thermohistoscreen Cassettes (DeMint, Valerie S) 4. Bone, Biomaterials & Medical Device Implants Histology Event In Cambridge, MA - May 4th, 2013 (Jack Ratliff) 5. (no subject) (Karla Arrington) 6. Glucose Oxidase blocking method (Long, Denise) 7. Home based slide interpretation (Jennifer MacDonald) 8. Immunohistochemistry for Adipose tissue macrophages in humans (khadija irfan) 9. Re: Home based slide interpretation (Rene J Buesa) 10. Picrosirius red on cryosections (Laura Avogaro) 11. RE: Picrosirius red on cryosections (Elizabeth Chlipala) 12. RELIA Hot Job Alert 4-16-2013 Exciting Opportunities in NC.VA, GA, CA, CO, IL, TX and NM (Pam Barker) 13. SP and CGRP antibodies (Dessoye, Michael J) 14. GSH Symposium (Deloris Carter) 15. RE: GSH Symposium (Shirley A. Powell) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Apr 2013 17:39:26 +0000 From: "Hale, Meredith" Subject: [Histonet] Blades To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0E828EC51C7CC445A51E53F81B64E8C721A8B5@s-irv-exchmb.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> ------------------------------ Message: 2 Date: Mon, 15 Apr 2013 12:48:46 -0500 From: "Henry Chidgey" Subject: [Histonet] Question regarding appropriate use of histonet To: Message-ID: <005801ce3a01$7c705d00$75511700$@net> Content-Type: text/plain; charset="us-ascii" Howdy Folks, As I am sure you can tell we are subscribers to histonet and appreciate the generosity of members in helping solve the occasional problem we encounter. We are in need of finding either an individual or lab in our area to perform mammal root sectioning and slide preparation for the forensic mammal aging business we operate located near Burnet, Texas in the Texas Hill Country. I would like to reach out to your subscriber base to see if anyone is interested in doing some part time work for us or know someone who might be. Please let me know if that would be an appropriate use of the site. Thanks, Henry Chidgey CoFounder See what folks are saying about us Wildlife Analytical Laboratories www.DeerAge.com PO Box 295 1303 CR 118 B Burnet, Texas 78611 (512) 756-1989 (512) 663-9476 Cell Serving Wildlife Stewards, Ranchers & Hunters Worldwide Customer Service and Quality of Analysis are our Priority and Passion Facebook Eagle Soybeans ------------------------------ Message: 3 Date: Mon, 15 Apr 2013 13:09:10 -0500 From: "DeMint, Valerie S" Subject: [Histonet] Thermohistoscreen Cassettes To: Message-ID: <657B95ED46A98B4D9F44EFC67EDCAADD07477024@RWDEX3.WHS.phci.org> Content-Type: text/plain; charset="us-ascii" Is anyone waiting for their backorder of Thermo Histoscreen Cassettes? We no longer use this cassette and I am trying to sell my remaining inventory. I have several colors available. Write if you want more information :-) Valerie DeMint, HT(ASCP) Waukesha Memorial Hospital Waukesha WI This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 4 Date: Mon, 15 Apr 2013 14:14:28 -0400 From: Jack Ratliff Subject: [Histonet] Bone, Biomaterials & Medical Device Implants Histology Event In Cambridge, MA - May 4th, 2013 To: Histonet Cc: Jack Ratliff , Jack Ratliff Message-ID: Content-Type: text/plain; charset="iso-8859-1" If you are currently working with bone, biomaterials or medical device implants, you won't want to miss this specialty histology event http://www.polysciences.com/Interactive-Histology-Forum-About/185/ sponsored by Polysciences Inc. and supported with six (6) continuing education units (CEU's) by the National Society for Histotechnology! There is still time to pre-register online http://events.r20.constantcontact.com/register/event?llr=gqebcrbab&oeidk=a07e6rs5o33629b055e or onsite May 4th at the Hyatt Regency Cambridge http://www.polysciences.com/Interactive-Histology-Forum-Hotel-Travel/189/. Topics of discussion will include: A Closer Look at Fixation, Decalcification and Processing Techniques for Paraffin Embedded SpecimensPresented by Robert A. Skinner, HTL (ASCP), Director of Laboratory Resources, Center for Orthopaedic Research, University of Arkansas for Medical Sciences Histology Technique Selection and Challenges for Biomaterial and Medical Device Implanted TissuesPresented by Philip Seifert, MS, HTL (ASCP), Principal Scientist, CBSET, Inc. Acrylic Resins: A Practical Approach for Demonstrating Undermineralized Bone, Biomaterials and Medical Device ImplantsPresented by Jack L. Ratliff, BA, Owner, Ratliff Histology Consultants & Chairman, Hard Tissue Committee National Society for Histotechnology The Use Of A Non-Contact Laser To Collect Thin Sections From A Variety of Soft And Hard Histological Tissues, Synthetic Bio-Resorbable Materials, and Tissues Containing Non-Resorbable Medical Device ImplantsPresented by Heiko Richter, Ph.D., Sales & Product Management, Rowiak GmbH Introduction to Routine Staining and Special Staining with Plastic Resin TechniquesPresented by Valantou Grover, HT (ASCP) HTL, PA, Product Line Manager, Business Manager, Histologist at Polysciences, Inc. If you have any questions, please feel free to contact myself or Ashley Gidzinski (Ashley.Gidzinski@polysciences.com). We look forward to seeing you in Cambridge, MA and May the 4th be with you! Best Regards, Jack Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology ------------------------------ Message: 5 Date: Mon, 15 Apr 2013 13:04:21 -0700 (PDT) From: Karla Arrington Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" Message-ID: <1366056261.91439.YahooMailNeo@web121901.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii How do I boost the power of mucicarmine for staining goblet cells? I have opened a new lot/bottle and have used full strength but it is not staining the cells. Thanks, Karla ------------------------------ Message: 6 Date: Mon, 15 Apr 2013 21:18:07 +0000 From: "Long, Denise" Subject: [Histonet] Glucose Oxidase blocking method To: "histonet@lists.utsouthwestern.edu" Message-ID: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F72A572@MAILD.grove.ad.uconn.edu> Content-Type: text/plain; charset="us-ascii" Hello, I'm looking for updated information on the Glucose Oxidase blocking method. In particular, what reagents are being used now that Sigma's G5250 is no longer available. Does anyone know of a company that has this as a kit? Thanks, Denise Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ------------------------------ Message: 7 Date: Mon, 15 Apr 2013 15:12:23 -0700 From: Jennifer MacDonald Subject: [Histonet] Home based slide interpretation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Does anyone know of any regulations against a Pathologist reading slides from home? If so would you be able to cite the specific regulation? Thanks, Jennifer ------------------------------ Message: 8 Date: Mon, 15 Apr 2013 22:19:39 -0700 (PDT) From: khadija irfan Subject: [Histonet] Immunohistochemistry for Adipose tissue macrophages in humans To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1366089579.51528.YahooMailNeo@web122204.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello All ? I am a clinical researcher so my questions may be somewhat naive, but I would be extremely grateful for any help. I want to look at adipose tissue macrophage polarity by immunohistochemistry for activation markers. I have the following questions: 1. How much tissue sample is required. I want to use subcutaneous and omental fat samples. 2. How should I transport and store my samples. How long can I store them? I anticipate that I would be collecting samples over 2-3 months. 3. If I do H&E staining first, can I do immunohistochemistry on the same slide? Can I do successive markers on the same slide? How many markers can I use it for? ? Thanks ? Khadija ------------------------------ Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald , "histonet@lists.utsouthwestern.edu" Message-ID: <1366119506.58355.YahooMailNeo@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. From: Jennifer MacDonald To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 16 Apr 2013 16:30:14 +0200 (CEST) From: "Laura Avogaro" Subject: [Histonet] Picrosirius red on cryosections To: histonet@lists.utsouthwestern.edu Message-ID: <2828.192.168.178.78.1366122614.squirrel@www.science.unitn.it> Content-Type: text/plain;charset=iso-8859-1 Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy Tel: +39 0461 283425 ------------------------------ Message: 11 Date: Tue, 16 Apr 2013 08:42:25 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] Picrosirius red on cryosections To: Laura Avogaro , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2AE3@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Laura This type of analysis does not need to be completed on frozen sections it can be performed on routine 10% NBF fixed paraffin sections. There are protocols on line on the website stains file. Here is the link http://stainsfile.info/StainsFile/stain/conektv/puchtler_psr.htm I would let the frozen sections air dry for at least 30 minutes and then post fix in 10% NBF for about 10 minutes and then start this procedure. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laura Avogaro Sent: Tuesday, April 16, 2013 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Picrosirius red on cryosections Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) - Italy Tel: +39 0461 283425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 16 Apr 2013 11:32:41 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Hot Job Alert 4-16-2013 Exciting Opportunities in NC.VA, GA, CA, CO, IL, TX and NM To: "Histonet" Message-ID: <0b2801ce3ab7$a2bec300$e83c4900$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I hope you enjoyed a beautiful Spring weekend. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance and most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent The histology positions that I am most excited about are located in these areas: Charlotte, NC Salem, VA Staunton, VA Atlanta, GA Long Beach, CA Denver, CO Chicago, IL Springfield, IL Tyler, TX Waco, TX Roswell, NM Long Island, NY Syracuse, NY Louisville, KY East of Columbus, OH If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1@earthlink.net or you can always catch me on cell via call or text at 407-353-5070. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 13 Date: Tue, 16 Apr 2013 11:53:45 -0400 From: "Dessoye, Michael J" Subject: [Histonet] SP and CGRP antibodies To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello everyone, Is anyone out there using a commercially available antibody for SP (Substance P) and CGRP (Calcitonin gene-related peptide)? If so, where do you obtain it? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 14 Date: Tue, 16 Apr 2013 11:30:26 -0500 From: Deloris Carter Subject: [Histonet] GSH Symposium To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party! Deloris Carter ------------------------------ Message: 15 Date: Tue, 16 Apr 2013 12:52:11 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] GSH Symposium To: Deloris Carter , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE25799E77BB2@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" GSH would like to thank you for coming. Glad you had a good time and we will let you know when the next meeting is and the dates as soon as we get them. Crash our party anytime. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Tuesday, April 16, 2013 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GSH Symposium I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party! Deloris Carter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 16 ***************************************** Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From becky.garrison <@t> jax.ufl.edu Tue Apr 16 14:17:26 2013 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Tue Apr 16 14:17:32 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: References: Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F240B876E1@JX1B-MAIL1.umc.ufl.edu> We store at room temperature in a flammable cabinet. Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a container of isopentane in a container of Liquid Nitrogen. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plott <@t> uab.edu Tue Apr 16 14:18:07 2013 From: plott <@t> uab.edu (Patricia F Lott) Date: Tue Apr 16 14:18:12 2013 Subject: [Histonet] need plastic bottle for VIP 3000 Message-ID: <372C4AF089B6AE48B36F064FA891FF78137AB292@UABEXMB3.ad.uab.edu> Does anyone have an extra plastic solution bottle for a VIP 3000? We bought a used one, and one of the bottles leaks! Please contact: Patty Lott 205-934-2007 From mbmphoto <@t> gmail.com Tue Apr 16 14:40:17 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Apr 16 14:40:22 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: References: Message-ID: <074CFC3F-02CB-4845-BB2B-23DFA44E82F1@gmail.com> Hello Paula, At my previous lab at UCSF, we often had a lot of large & small tissue to freeze & therefore we used quite a bit of liquid nitrogen. We would get our LN2 from a local vendo, & was delivered in a low pressure 230 tall slender LN2 tank. The tank had a nozzle attached for easy & safe dispensing. We then dispensed LN2 into 1 and 2 liter Dewar flasks. These flasks were keep at room temp (under the hood), ready to use during freezing. This 230 liter tank was strapped stored in a corner of the lab (room temp) & the LN2 inside the tank would last about 3 weeks. The LN2 vendor automatically made delivers of LN2 to our lab - once a month & cost under $100/month. This local LN2 vendor, also carries smaller tanks filled with LN2 which can be stored at room temp., with dispensing nozzles. Paula, this is another option for you - but you'd still need to purchase a Dewar flask to use during freezing. Hope all is well. Best Maria Mejia Affymetrix, Inc On Apr 16, 2013, at 10:35 AM, Paula Sicurello wrote: > Hello HistoNetters, > > I have been having a discussion with my boss about whether or not to store > the isopentane (used for freezing muscle biopsies) in the refrigerator. (An > expensive, explosion proof one that I would have to buy.) > > Her only experience is with the isopentane stored in the refrigerator, my > experiences are with storing it at room temperature. > > What do you all do? Room temp or refrigerated? > > Any and all comments are greatly appreciated. > > Thanks! > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Apr 16 14:56:06 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Apr 16 14:56:17 2013 Subject: [Histonet] Primera slide printer Message-ID: <493CAA64F203E14E8823737B9EE0E25F093D1968A4@EXCHMB01.isis.local> Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From lblazek <@t> digestivespecialists.com Tue Apr 16 15:00:46 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 16 15:03:13 2013 Subject: [Histonet] RE: Primera slide printer In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F093D1968A4@EXCHMB01.isis.local> References: <493CAA64F203E14E8823737B9EE0E25F093D1968A4@EXCHMB01.isis.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E39165278D3FD@IBMB7Exchange.digestivespecialists.com> Bea, You can use need to use the clipped corner slides. The best ones are the Epic Scientific slides available from CWS, Inc or the clipped corner Tanner slides available from Mercedes Medical. I'm sure others carry them also. These are the only two slides I've found that work really well in the printer. I am very happy with my slide printer. Set up was very simple and interfacing with our LIS was very easy. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, April 16, 2013 3:56 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Primera slide printer Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Apr 16 15:35:14 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Apr 16 15:35:26 2013 Subject: [Histonet] Isopentane storage-the polling is open Message-ID: <000301ce3ae1$e835dde0$b8a199a0$@bresnan.net> We stored at RT but lab did not have a flammable storage cabinet . Since we did not have an explosion proof refrigerator or cold room, it was deemed too dangerous to store in those areas. There is good reason why one doesn't store in regular refrigerators/cold room. Histologic, Vol. XXXVI, No.1, May 2003, Robert Skinner and Dana Gaddy article t"More than Just A Mandatory Excercise" to see the total, costly ($200,000) destruction of a laboratory at University of Arkansas as the result of a loose lid on an isopentane bottle stored in a non-explosion proof freezer. Fortunately this happened in very early morning when no one was in the lab. It is also not good to breathe the isopentane fumes so working inside a hood is mandatory. We eventually discontinued use of isopentane (2 methyl butane) and used a simple method with LN2 cooled petri dish (supported on a platform) in a styrofoam box for snap freezing multiple tissue blocks (as many as 40) in a tissue dissection/collection session. 1. If you have to have it in lab without an explosion proof refrigerator or freezer, store at RT in flammable cabinet. Gayle Callis HTL/HT/MT(ASCP) From estellamireles <@t> gmail.com Tue Apr 16 15:53:43 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Apr 16 15:53:48 2013 Subject: [Histonet] Scanning routine slides for Path. Message-ID: *I need to gather information on any instrumentation that will enable me to digitally scan routine slides and send them to my Dr.* *We are at the beginning of our quest to place one in the lab.* *I am really interested in what my fellow colleagues opinions is concerning their own experience.* ** *Thanks* Stella W. Houston, Tx From lpwenk <@t> sbcglobal.net Tue Apr 16 19:33:14 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Apr 16 19:33:21 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: <761E2B5697F795489C8710BCC72141FF079621@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF079621@ex07.net.ucsf.edu> Message-ID: <66D57C82FCCC48F8B38FAE91D3F39554@HP2010> Definitely make it explosion-proof. See the following article by Robert Skinner in Sakura's Histo-Logic. http://www.sakura-americas.com/histologic/pdf/03_may.pdf He had an explosion in a freezer, when a researcher tried to store 50 mL (about $2.00) worth of isopentane in a regular freezer, and the lid on the container wasn't on tight enough. Fortunately, it was the middle of the night, and no one was in the room. The freezer door flew 12 feet, and there was lots of flames. See the photos! The article mentions the damage at $165,000, but I talked with Robert about a year later, and he said the total was over $1,000,000 when everything was factored in. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my place of employment. -----Original Message----- From: Morken, Timothy Sent: Tuesday, April 16, 2013 2:32 PM To: Paula Sicurello ; HistoNet Subject: RE: [Histonet] Isopentane storage-the polling is open Paula, we keep ours at room temp in a small flam cab near the muscle bench. We keep two gallons on hand. In Dapson & Dapson's book "Hazardous Materials in the Histopathology Laboratory" (Anatech, 2005) they do not make any specific recommendations concerning storage temperature, except to say IF it is kept in the refrigerator/freezer use only an explosion-proof refrigerator/freezer. The flash point is -50C, so a fridge is not significantly different than room temp, unless your lab is often over 30C/85F (the boiling point!). Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 10:35 AM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Apr 16 20:02:06 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 16 20:02:10 2013 Subject: [Histonet] CSH Room rates extended Message-ID: If you are interested in attending the CSH Symposium May 3-5 the room rate date has been extended. The CSH rate for rooms will expire April 19. Book now. Some great workshops and networking opportunities. http://californiahistology.org/events.html From mwhite <@t> mcleodhealth.org Wed Apr 17 07:41:39 2013 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Wed Apr 17 07:41:43 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: <66D57C82FCCC48F8B38FAE91D3F39554@HP2010> References: <761E2B5697F795489C8710BCC72141FF079621@ex07.net.ucsf.edu> <66D57C82FCCC48F8B38FAE91D3F39554@HP2010> Message-ID: For almost two years, we have been using- with great success- a much safer alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a paid endorser and these are my views only , and do not reflect in any way on my employer. Our pathologists greatly prefer the results of a freezing bath over the cryostat peltier method. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. From b-frederick <@t> northwestern.edu Wed Apr 17 07:42:12 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Apr 17 07:42:17 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: <9E47DE9D490DCC42A2EAE94F22BF93F240B876E1@JX1B-MAIL1.umc.ufl.edu> References: <9E47DE9D490DCC42A2EAE94F22BF93F240B876E1@JX1B-MAIL1.umc.ufl.edu> Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D199C1@evcspmbx2.ads.northwestern.edu> Always in an explosion proof refrigerator for storage. The isopentane in use is in an isopentane tank (at -70C) in the frozen section area of the gross room as is the fridge.... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky Sent: Tuesday, April 16, 2013 2:17 PM To: 'Paula Sicurello'; HistoNet Subject: RE: [Histonet] Isopentane storage-the polling is open We store at room temperature in a flammable cabinet. Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a container of isopentane in a container of Liquid Nitrogen. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mstone <@t> cmhlink.org Wed Apr 17 08:37:50 2013 From: mstone <@t> cmhlink.org (MARCELLYN A. STONE) Date: Wed Apr 17 08:37:57 2013 Subject: [Histonet] Sakura film coverslipper Message-ID: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF740D1@exch-mbx-01.corp.cmhlink.org> Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. From LBUSTAMANTE <@t> cvm.tamu.edu Wed Apr 17 08:44:01 2013 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Wed Apr 17 08:44:05 2013 Subject: [Histonet] Green stain for Fat Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A14D3@CVMMB02.cvm.tamu.edu> Does anyone knows any fat stain were the fat stains green? Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From rjbuesa <@t> yahoo.com Wed Apr 17 08:45:15 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 17 08:45:18 2013 Subject: [Histonet] Sakura film coverslipper In-Reply-To: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF740D1@exch-mbx-01.corp.cmhlink.org> References: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF740D1@exch-mbx-01.corp.cmhlink.org> Message-ID: <1366206315.85349.YahooMailNeo@web163104.mail.bf1.yahoo.com> Personally I prefer film coverslipper and used the one by Sakura. Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. The other "disadvantage" some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of?any glass coverslip. Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. Ren? J. From: MARCELLYN A. STONE To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, April 17, 2013 9:37 AM Subject: [Histonet] Sakura film coverslipper Good morning ? I am looking into buying a film coverslipper from Sakura.? We currently use a glass coverslipper.? I would like any thoughts, pros, cons that you may have on film vs. glass.? I have heard that there tends to be a problem a few years later with the film curling.? Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 17 08:46:49 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 17 08:46:53 2013 Subject: [Histonet] Green stain for Fat In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A14D3@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A14D3@CVMMB02.cvm.tamu.edu> Message-ID: <1366206409.1424.YahooMailNeo@web163103.mail.bf1.yahoo.com> It would have to be a stain that dissolves in fat and I do not know of any with such characteristic. If you find one, please advise everybody in the list. Ren? J. From: "Bustamante, Lin" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, April 17, 2013 9:44 AM Subject: [Histonet] Green stain for Fat Does anyone knows any fat stain were the fat stains green? Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Smith_D <@t> kids.wustl.edu Wed Apr 17 09:07:59 2013 From: Smith_D <@t> kids.wustl.edu (Smith, Denise) Date: Wed Apr 17 09:08:09 2013 Subject: [Histonet] HTL Exam Message-ID: <7578651EA53E304B96B06AD6D7B8992C196BBD@pcfmbx01.wusm-pcf.wustl.edu> Hi, I'm new around here! I am currently a research technician but want to pursue my career into Histology since I have been doing sectioning, stainings, and embedding. I have been studying for HTL exam and will take it soon. I have discussed with Histology Core at my work and they highly recommended for me to take HTL exam instead of HT because of my degree and experience. I'm wondering if anyone recently has took the HTL exam and how did it go? Was it harder than you thought it was? What materials should I focus on the most? I have checked on ASCP website and printed out study guide. I do have "Histotechnology A Self Instructional Text" 3rd edition. I'm getting more nervous because I don't want to fail it at first time. Thank you, Denise Smith smith_d@kids.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From rjbuesa <@t> yahoo.com Wed Apr 17 08:35:16 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 17 09:31:18 2013 Subject: [Histonet] Re: Home based slide interpretation In-Reply-To: References: Message-ID: <1366205716.69850.YahooMailNeo@web163101.mail.bf1.yahoo.com> John: You are absolutely right! That is also an issue. Its seems that with every bit of additional information the case about being illegal for a pathologist to read slides from home is building up more and more strong! Very interesting. Ren? J. From: John Spaull To: "rjbuesa@yahoo.com" ; "JMacDonald@mtsac.edu" Sent: Wednesday, April 17, 2013 2:44 AM Subject: Home based slide interpretation The UK Human Tissues Act (2006) is also relevant I think. To work with human tissue premises have to be registered. All tissue is tracked. The Health Service may have exemptions but generally tracking requires approved couriers etc. Registration of premises does require payment of a fee. ? One can imagine the headlines if your home working pathologist was transporting slides themselves and say left diagnostic samples on the train. Patient confidentiality around physical notes would also be something to be considered I guess. ? ------------------------------ ? Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald , ????? "histonet@lists.utsouthwestern.edu" ????? Message-ID: ????? <1366119506.58355.YahooMailNeo@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. ? From: Jennifer MacDonald To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation ? ? Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? Regards, ? John. ? John Spaull Histology Group, Molecular and Cellular Technologies, Platform Technology and Science, GlaxoSmithKline R&D, Stevenage, SG1 2NY, UK +44 (0)1438 763296 E-mail john.r.spaull@gsk.com ? ? This e-mail was sent by GlaxoSmithKline Services Unlimited (registered in England and Wales No. 1047315), which is a member of the GlaxoSmithKline group of companies. The registered address of GlaxoSmithKline Services Unlimited is 980 Great West Road, Brentford, Middlesex TW8 9GS. From DSiena <@t> statlab.com Wed Apr 17 09:41:22 2013 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Apr 17 09:39:18 2013 Subject: [Histonet] Re: Home based slide interpretation In-Reply-To: <1366205716.69850.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1366205716.69850.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: It is my understanding that whenever the reading of the slides is not performed at the testing site that they need to list that on their report and also they will need to have a CLIA license for their home or office that is offsite. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 8:35 AM To: John Spaull; JMacDonald@mtsac.edu Cc: Histonet Subject: [Histonet] Re: Home based slide interpretation John: You are absolutely right! That is also an issue. Its seems that with every bit of additional information the case about being illegal for a pathologist to read slides from home is building up more and more strong! Very interesting. Ren? J. From: John Spaull To: "rjbuesa@yahoo.com" ; "JMacDonald@mtsac.edu" Sent: Wednesday, April 17, 2013 2:44 AM Subject: Home based slide interpretation The UK Human Tissues Act (2006) is also relevant I think. To work with human tissue premises have to be registered. All tissue is tracked. The Health Service may have exemptions but generally tracking requires approved couriers etc. Registration of premises does require payment of a fee. ? One can imagine the headlines if your home working pathologist was transporting slides themselves and say left diagnostic samples on the train. Patient confidentiality around physical notes would also be something to be considered I guess. ? ------------------------------ ? Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald , ????? "histonet@lists.utsouthwestern.edu" ????? Message-ID: ????? <1366119506.58355.YahooMailNeo@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. ? From: Jennifer MacDonald To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation ? ? Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? Regards, ? John. ? John Spaull Histology Group, Molecular and Cellular Technologies, Platform Technology and Science, GlaxoSmithKline R&D, Stevenage, SG1 2NY, UK +44 (0)1438 763296 E-mail john.r.spaull@gsk.com ? ? This e-mail was sent by GlaxoSmithKline Services Unlimited (registered in England and Wales No. 1047315), which is a member of the GlaxoSmithKline group of companies. The registered address of GlaxoSmithKline Services Unlimited is 980 Great West Road, Brentford, Middlesex TW8 9GS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Wed Apr 17 09:39:56 2013 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Wed Apr 17 09:39:56 2013 Subject: [Histonet] Fw: Isomet 2000 Precision Saw (s) Message-ID: <6A4D3A41B9844D2590F36EB3E8FACE89@vetmed.wisc.edu> Sent: Tuesday, April 16, 2013 7:54 AM Subject: Isomet 2000 Precision Saw (s) I am interested in 1-2 replacement saws of this OLDER model, using the load system. Must be in good working condition. Thanks! 608-262-8534. Vicki Kalscheur Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 kalschev@svm.vetmed.wisc.edu From Lynn.Burton <@t> Illinois.gov Wed Apr 17 10:42:20 2013 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Apr 17 10:42:29 2013 Subject: [Histonet] RE: Sakura film coverslipper In-Reply-To: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF740D1@exch-mbx-01.corp.cmhlink.org> References: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF740D1@exch-mbx-01.corp.cmhlink.org> Message-ID: We have had a Sakura film coverslipper since 1997. It has had three service calls, mostly in last 5 years. Those service calls were minor problems quickly fixed. When we demoed several different coverslippers, we found this one to have the least amount of "tweeking" involved. It has been a wonderful machine and Tech One services it. They say it is a great machine. If I were buying again; I would go straight to this. Lynn Burton Animal Disease Laboratory Galesburg, Il -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN A. STONE Sent: Wednesday, April 17, 2013 8:38 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBUSTAMANTE <@t> cvm.tamu.edu Wed Apr 17 10:45:12 2013 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Wed Apr 17 10:45:23 2013 Subject: [Histonet] Pituitary gland Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From rjbuesa <@t> yahoo.com Wed Apr 17 10:52:44 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 17 10:52:51 2013 Subject: [Histonet] Pituitary gland In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> Message-ID: <1366213964.25928.YahooMailNeo@web163104.mail.bf1.yahoo.com> Use Mallory-Azan trichrome stain. It was specifically design for pituitary gland. You can find the procedure in any good histotechnology book. Ren? J. From: "Bustamante, Lin" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, April 17, 2013 11:45 AM Subject: [Histonet] Pituitary gland Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Apr 17 10:59:02 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Apr 17 10:59:08 2013 Subject: [Histonet] Re: Isopentane storage... Message-ID: <9F3CFEE76E51B64991C7485270890B404973D81F@EX5.lj.gnf.org> Melanie White wrote: For almost two years, we have been using- with great success- a much safer alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a paid endorser and these are my views only , and do not reflect in any way on my employer. Our pathologists greatly prefer the results of a freezing bath over the cryostat peltier method. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 Ditto what she said about the Novec fluid. It works just as well, without the storage hazard. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From lharris <@t> samhealth.org Wed Apr 17 11:25:14 2013 From: lharris <@t> samhealth.org (Lori Harris) Date: Wed Apr 17 11:25:23 2013 Subject: [Histonet] RE: Primera slide printer In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39165278D3FD@IBMB7Exchange.digestivespecialists.com> References: <493CAA64F203E14E8823737B9EE0E25F093D1968A4@EXCHMB01.isis.local> <5A2BD13465E061429D6455C8D6B40E39165278D3FD@IBMB7Exchange.digestivespecialists.com> Message-ID: <450EDC37E404D142AF67D7314C954C8A3C799D7EB7@SHSMAILVI01.int.samhealth.net> Bea, Ditto what Linda said! Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 16, 2013 1:01 PM To: Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Primera slide printer Bea, You can use need to use the clipped corner slides. The best ones are the Epic Scientific slides available from CWS, Inc or the clipped corner Tanner slides available from Mercedes Medical. I'm sure others carry them also. These are the only two slides I've found that work really well in the printer. I am very happy with my slide printer. Set up was very simple and interfacing with our LIS was very easy. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, April 16, 2013 3:56 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Primera slide printer Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From BDeBrosse-Serra <@t> isisph.com Wed Apr 17 11:26:11 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Apr 17 11:26:16 2013 Subject: [Histonet] RE: Primera slide printer In-Reply-To: <450EDC37E404D142AF67D7314C954C8A3C799D7EB7@SHSMAILVI01.int.samhealth.net> References: <493CAA64F203E14E8823737B9EE0E25F093D1968A4@EXCHMB01.isis.local> <5A2BD13465E061429D6455C8D6B40E39165278D3FD@IBMB7Exchange.digestivespecialists.com> <450EDC37E404D142AF67D7314C954C8A3C799D7EB7@SHSMAILVI01.int.samhealth.net> Message-ID: <493CAA64F203E14E8823737B9EE0E25F093D1968B0@EXCHMB01.isis.local> Thank you guys for all the feedback! It is always greatly appreciated! Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: Lori Harris [mailto:lharris@samhealth.org] Sent: Wednesday, April 17, 2013 9:25 AM To: Blazek, Linda; Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: RE: Primera slide printer Bea, Ditto what Linda said! Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 16, 2013 1:01 PM To: Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Primera slide printer Bea, You can use need to use the clipped corner slides. The best ones are the Epic Scientific slides available from CWS, Inc or the clipped corner Tanner slides available from Mercedes Medical. I'm sure others carry them also. These are the only two slides I've found that work really well in the printer. I am very happy with my slide printer. Set up was very simple and interfacing with our LIS was very easy. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, April 16, 2013 3:56 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Primera slide printer Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lharris <@t> samhealth.org Wed Apr 17 11:31:05 2013 From: lharris <@t> samhealth.org (Lori Harris) Date: Wed Apr 17 11:31:10 2013 Subject: [Histonet] Sakura film coverslipper In-Reply-To: <1366206315.85349.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF740D1@exch-mbx-01.corp.cmhlink.org> <1366206315.85349.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <450EDC37E404D142AF67D7314C954C8A3C799D7EC4@SHSMAILVI01.int.samhealth.net> Marcellyn, I agree with Rene. I have always worked in Histology Labs that have used the Sakura tape coverslipper and have not had any problems. If the amount of xylene that is dispensed is adequate, you should have no problems with the coverslips peeling off over time. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 6:45 AM To: MARCELLYN A. STONE; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Sakura film coverslipper Personally I prefer film coverslipper and used the one by Sakura. Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. The other "disadvantage" some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of any glass coverslip. Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. Ren? J. From: MARCELLYN A. STONE To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, April 17, 2013 9:37 AM Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mwhite <@t> mcleodhealth.org Wed Apr 17 12:08:02 2013 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Wed Apr 17 12:08:09 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: <66D57C82FCCC48F8B38FAE91D3F39554@HP2010> References: <761E2B5697F795489C8710BCC72141FF079621@ex07.net.ucsf.edu> <66D57C82FCCC48F8B38FAE91D3F39554@HP2010> Message-ID: I just wanted to clarify that we don't use Novec 7000 for freezing of muscle tissue. We use it for other routine frozen sections on humans.....Sorry if that caused any confusion. Thanks Tim for pointing that out! Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. From justin.wood <@t> arrowheaddermatology.com Wed Apr 17 13:35:49 2013 From: justin.wood <@t> arrowheaddermatology.com (Justin Wood) Date: Wed Apr 17 13:37:22 2013 Subject: [Histonet] Job opening Message-ID: <005001ce3b9a$6217eaa0$2647bfe0$@arrowheaddermatology.com> Currently seeking histo-tech for Dermatology office that is opening up own path lab. Lab will be opening mid May. If interested please reply with resume and current number to reach you. Justin D.Wood Executive Director Arrowhead Dermatology, PC 623-487-3003 Phone 623-487-0952 Fax CONFIDENTIALITY NOTICE: The information contained in this email and any attachments is confidential and intended solely for the use of the persons named above. If the reader of this message is not the intended recipient, or employee or agent responsible for delivering it to the intended recipient, you are requested not to read, distribute, copy or otherwise use it. If you have received this communication in error, please immediately notify the sender by telephone or email, and destroy this message and any attachments. From nelsonrnch <@t> verizon.net Wed Apr 17 15:06:17 2013 From: nelsonrnch <@t> verizon.net (PATTI NELSON HT(ASCP)) Date: Wed Apr 17 15:06:22 2013 Subject: [Histonet] HT/HTL With MOHs Message-ID: <1366229177.90976.YahooMailNeo@web84501.mail.ne1.yahoo.com> ? To All, ***Great opportunity in Long Beach,?California.? Derm Lab?is looking for a certified HT or HTL?with MOH's experience?to manage their?new laboratory. Candidate must be ASCP certified and eligible to perform gross dissection under the CLIA regulations. Responsibilities would also include the following: Maintenance/editing of policies and procedures to State/CLIA standards, leading lab through State/CLIA inspection, maintenance?,?QA?and QC?for equipment and routine histology duties, such as? patient accessioning, grossing, embedding, cutting, staining to cover-slipping and troubleshooting. This is a?full time?position that offers a competitive rate and flexible hours.? Interested applicants should contact me at your earliest convenience. Please privately in-box me your resumes/CV. ? ? ? BEST REGARDS, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From justin.wood <@t> arrowheaddermatology.com Wed Apr 17 15:17:59 2013 From: justin.wood <@t> arrowheaddermatology.com (Justin Wood) Date: Wed Apr 17 15:19:27 2013 Subject: [Histonet] Job posting Message-ID: <006301ce3ba8$a8408bf0$f8c1a3d0$@arrowheaddermatology.com> We are currently looking for Histology Techs. Experienced professionals will have an understanding of Anatomic and Clinical laboratory services for Histology. .Strong working knowledge with performance of sampling, accessioning and grossing, tissue processing and staining ,QC , .Experience with instrument calibration and validation, Testing management .Encompassing functions toward the attainment of quality testing within the laboratory. .Performs accessioning and receipt of specimens. .Tissue preparation to include grossing, processing, embedding, cutting and staining .Confirmation with pathologist of appropriate quality control material for use in the diagnosis and treatment of disease. .Perform special stains and immunohistological procedures to include all aspects of antigen retrieval, processing and staining to include the quality control and validation .Meets all the responsibility requirements of histology technologist according to state and federal law. .Assists laboratory management in the daily operation as assigned. Minimum Requirements .BS or BA in Biological Science with intended focus. .Two years experience with histology and cytology procedures with:. routine, special stains and Immunohistochemistry procedures. (IHC). .Certifications: (ASCP) Histotechnologist (HT) or eligible Please forward your resume and contact info to justin.wood@arrowheaddermatology.com Justin D.Wood Executive Director Arrowhead Dermatology, PC 623-487-3003 Phone 623-487-0952 Fax CONFIDENTIALITY NOTICE: The information contained in this email and any attachments is confidential and intended solely for the use of the persons named above. If the reader of this message is not the intended recipient, or employee or agent responsible for delivering it to the intended recipient, you are requested not to read, distribute, copy or otherwise use it. If you have received this communication in error, please immediately notify the sender by telephone or email, and destroy this message and any attachments. From kiran_g <@t> sbcglobal.net Wed Apr 17 16:25:36 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Wed Apr 17 16:25:40 2013 Subject: [Histonet] AutoTech Embedder( Sakura) Message-ID: <1366233936.39505.YahooMailClassic@web184401.mail.bf1.yahoo.com> Hello Dear Colleagues, ? We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. ? Anyone currenly using?it and would like to share their experience is great. ? Thank you, ? -Kiran From hlukey <@t> msn.com Wed Apr 17 19:18:31 2013 From: hlukey <@t> msn.com (Hugh Luk) Date: Wed Apr 17 19:18:37 2013 Subject: [Histonet] Sakura film coverslipper In-Reply-To: References: Message-ID: Marcellyn, We have never owned a film coverslipper because in Hawaii, the only medical center with a film instrument ($) had problems after 5 years. The film pulled off the slides; meaning, they wanted to go back and re-cover tens of thousands of slides, in order to stay compliant. Apparently and more shockingly, they were told the problem was simply the "Humidity" in our area was too high for 'film'. However, in talking with our new (and wonderful) Sakura representative, I now think it was inaccurate to blame film coverslippers, especially after Rene's and Lori's endorsement. In the right hands with quality film and the right xylene coverage, I presume it would work in all settings (it's not like we're going to coverslip slides on the beach or something, although...). But we already have the Sakura Glas g2; it's a workhorse too! We love ours. Very few problems, but a little pricey. Worth the money if your volume is high, and your time is short. Hugh UH cancer center > ------------------------------ > > Date: Wed, 17 Apr 2013 09:31:05 -0700 > From: Lori Harris > Subject: RE: [Histonet] Sakura film coverslipper > To: Rene J Buesa , "MARCELLYN A. STONE" > , "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <450EDC37E404D142AF67D7314C954C8A3C799D7EC4@SHSMAILVI01.int.samhealth.net> > > Content-Type: text/plain; charset="iso-8859-1" > > Marcellyn, > > I agree with Rene. I have always worked in Histology Labs that have used the Sakura tape coverslipper and have not had any problems. If the amount of xylene that is dispensed is adequate, you should have no problems with the coverslips peeling off over time. > > > Lori A. Harris, HT (ASCP) > Histology Section Lead > GSRMC Pathology Lab > 3600 NW Samaritan Drive > Corvallis, OR 97330 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, April 17, 2013 6:45 AM > To: MARCELLYN A. STONE; 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] Sakura film coverslipper > > Personally I prefer film coverslipper and used the one by Sakura. > Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. > The other "disadvantage" some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of any glass coverslip. > Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. > Ren? J. > > From: MARCELLYN A. STONE > To: "'histonet@lists.utsouthwestern.edu'" > Sent: Wednesday, April 17, 2013 9:37 AM > Subject: [Histonet] Sakura film coverslipper > > Good morning > I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. > Thanks Marcy > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Apr 17 20:14:16 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Apr 17 20:15:07 2013 Subject: [Histonet] RE: Pituitary gland In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D241C4B@xmdb04.nch.kids> Some references that might be useful: Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. Read More: http://informahealthcare.com/doi/abs/10.3109/10520295909114648 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Thursday, 18 April 2013 1:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pituitary gland Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mcauliff <@t> umdnj.edu Thu Apr 18 09:28:53 2013 From: mcauliff <@t> umdnj.edu (Geoff) Date: Thu Apr 18 09:28:49 2013 Subject: [Histonet] RE: Pituitary gland In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D241C4B@xmdb04.nch.kids> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> <6D6BD1DE8A5571489398B392A38A71579D241C4B@xmdb04.nch.kids> Message-ID: <51700325.2020407@umdnj.edu> In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references that might be useful: > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > Read More: http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin > Sent: Thursday, 18 April 2013 1:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pituitary gland > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > Thank you. > Lin. > > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Ronald.Houston <@t> nationwidechildrens.org Thu Apr 18 09:47:40 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Apr 18 09:47:44 2013 Subject: [Histonet] RE: Pituitary gland In-Reply-To: <51700325.2020407@umdnj.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> <6D6BD1DE8A5571489398B392A38A71579D241C4B@xmdb04.nch.kids> <51700325.2020407@umdnj.edu> Message-ID: back in the days when I worked exclusively in neuropathology in Glasgow, the tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange fuchsin green) methods. I don't have the references to hand , although I believe the first was attributed to Tony Pearse, but can probably pull up the methods if needed Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Thursday, April 18, 2013 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Pituitary gland In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references that might be useful: > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > Read More: > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bustamante, Lin > Sent: Thursday, 18 April 2013 1:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pituitary gland > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > Thank you. > Lin. > > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu Apr 18 09:57:33 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Apr 18 09:57:38 2013 Subject: [Histonet] Placentas and AP In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5055F27B9D@BL2PRD0711MB434.namprd07.prod.outlook.com> References: <015d01ce39de$45302870$cf907950$@imagesbyhopper.com> <8A70A9B2ECDD084DACFE6C59FCF86D5055F27B9D@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: <032501ce3c45$0ef06a90$2cd13fb0$@imagesbyhopper.com> As a follow up to this question: If the placentas are not sent to AP for testing, are they listed on a tissue exception list? Thanks! Michelle -----Original Message----- Sent: Monday, April 15, 2013 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Placentas and AP I have been asked this set of questions and thought I would ask the experts! This is regarding sending placentas to the lab for AP evaluations: Is there an industry standard, CAP or Joint Commission requirement for sending placentas for AP evaluations? Such as send all for evaluations; send complicated ones only etc. If there are specific requirements, can you please give me the req as well? Thanks for reading and considering, Michelle _____ No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.3272 / Virus Database: 3162/6245 - Release Date: 04/14/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.3272 / Virus Database: 3162/6245 - Release Date: 04/14/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.3272 / Virus Database: 3162/6245 - Release Date: 04/14/13 From kiran_g <@t> sbcglobal.net Thu Apr 18 12:13:23 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Thu Apr 18 12:13:45 2013 Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) References: <1366233936.39505.YahooMailClassic@web184401.mail.bf1.yahoo.com> Message-ID: Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: > From: Kiranjit Grewal > Date: April 17, 2013, 2:25:36 PM PDT > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AutoTech Embedder( Sakura) > > Hello Dear Colleagues, > > We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. > > Anyone currently using it and would like to share their experience is great. > > Thank you, > > -Kiran > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Thu Apr 18 12:18:02 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Thu Apr 18 12:18:10 2013 Subject: [Histonet] New Lab In KY Message-ID: <0E828EC51C7CC445A51E53F81B64E8C72242CB@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From McKenzie.Emily <@t> mhsil.com Thu Apr 18 12:36:27 2013 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Thu Apr 18 12:36:37 2013 Subject: [Histonet] email Message-ID: Mckenzie.emily@mshil.com ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From McKenzie.Emily <@t> mhsil.com Thu Apr 18 12:42:37 2013 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Thu Apr 18 12:42:41 2013 Subject: [Histonet] Calcium Stains? Message-ID: We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From rjr6 <@t> psu.edu Thu Apr 18 13:15:15 2013 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Apr 18 13:15:39 2013 Subject: [Histonet] RE: Calcium Stains? In-Reply-To: References: Message-ID: I use Dahl's Calcium stain Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily Sent: Thursday, April 18, 2013 1:43 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Calcium Stains? We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Thu Apr 18 13:32:30 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Apr 18 13:32:36 2013 Subject: [Histonet] Biosafety in the Mohs Laboratory Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF7673A7@EX-MLB-03.ad.georgiahealth.edu> After being well acquainted with the CAP question, in regards to decontamination of cryostats, I would like some feedback as to how some of you perform this function. The lower cost cryostats do not contain a fumigation cycle but instruct the user to use alcohol and/or some type of germicidal spray for decontamination. I am hesitant about this and wondering if I'm just "old school" believing in formaldehyde fumigation for eliminating Hepatitis, HIV, and TB. In the cryostat manual, there is no mention of fumigation and the cleaner they suggest is not sold in the United States. Thanks in advance for your help! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From VHammel <@t> primecare.org Thu Apr 18 13:56:01 2013 From: VHammel <@t> primecare.org (Hammel, Vicky) Date: Thu Apr 18 13:56:10 2013 Subject: [Histonet] looking for control block of "Burkitt Lymphoma" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2F2326ADB@EXCHANGE2K7.staprimecare.org> I am looking for a tissue block that contains "Burkitt Lymphoma", I have tissue with H.pylori, melanoma and amyloid that I would be willing to trade. Thank you, Vicky Hammel HTL, ASCP Pathology Technical Consultant vhammel@primecare.org St. Alexius Medical Center Histology Laboratory 900 east Broadway Bismarck, ND 58506 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From POWELL_SA <@t> mercer.edu Thu Apr 18 14:42:58 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Apr 18 14:43:09 2013 Subject: [Histonet] IHC question Message-ID: <9BF995BC0E47744E9673A41486E24EE2579A9DDBFC@MERCERMAIL.MercerU.local> I need to talk to any histotechs/researchers out there who are doing IHC on shellfish or fish. Please contact me personally at powell_sa@mercer.edu. Also any vendors who sell detection systems for them, please contact me. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From wdgill04 <@t> gmail.com Thu Apr 18 15:32:11 2013 From: wdgill04 <@t> gmail.com (Wayne D Gill) Date: Thu Apr 18 15:32:36 2013 Subject: [Histonet] VCP-2001 cassette printer question about ink Message-ID: <9765A008-719C-4B5A-955F-22D8013CC9BE@gmail.com> Does anyone know where to buy the ink ribbon for the VCP 2001 cassette printer? Vendor and order # would be helpful. Thank you. -Wayne From mbourgeois <@t> finantempleton.com Thu Apr 18 16:11:50 2013 From: mbourgeois <@t> finantempleton.com (Michael Bourgeois) Date: Thu Apr 18 16:12:52 2013 Subject: [Histonet] atlanta employment Message-ID: <006601ce3c79$58100f80$08302e80$@com> Finan Templeton Dermatopathology Associates, is seeking an experienced, intelligent, positive, dynamic and certified histotechnician/histotechnologist to become a member of our extraordinary team. The ideal candidate functions well in a team environment, has exceptional histological skills, and possesses ASCP certification. We provide a full range of benefits for all full-time team members including a generous profit sharing/retirement plan, paid vacation/sick time, and medical, dental, and vision insurance plans. Part-time team members receive partial benefit packages based upon hours worked. All Finan Templeton team members receive generously competitive wages along with a commitment to an environment of care, integrity, and mutual respect. Michael Bourgeois Lab Manager/Human Resources Manager (404)851-1766 | Fax (404)851-1767 | mdb@finantempleton.com 6095 Barfield Road Suite 200 | Atlanta, GA 30342 From tony.henwood <@t> health.nsw.gov.au Thu Apr 18 17:54:19 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Apr 18 17:54:36 2013 Subject: [Histonet] RE: Pituitary gland In-Reply-To: <51700325.2020407@umdnj.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> <6D6BD1DE8A5571489398B392A38A71579D241C4B@xmdb04.nch.kids> <51700325.2020407@umdnj.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420E7@xmdb04.nch.kids> Oh I am getting too old (and I was trying to be modern!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Friday, 19 April 2013 12:29 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Pituitary gland In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references that might be useful: > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > Read More: > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bustamante, Lin > Sent: Thursday, 18 April 2013 1:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pituitary gland > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > Thank you. > Lin. > > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Apr 18 18:25:52 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Apr 18 18:26:05 2013 Subject: [Histonet] RE: Pituitary gland In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC9A95A158D@CVMMB02.cvm.tamu.edu> <6D6BD1DE8A5571489398B392A38A71579D241C4B@xmdb04.nch.kids> <51700325.2020407@umdnj.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420FD@xmdb04.nch.kids> Yep, I missed one reference (by Prof Pearse) which I agree is my preferred technique: Everson Pearse, A. G. (1950). Differential stain for the human and animal anterior hypophysis. Biotechnic & Histochemistry, 25(2), 95-102. And where I used the stain: Henwood, A.F., (1983) "Crossreactivity in Immunoperoxidase: Human Placental Lactogen and the Pituitary". N.Z. J. Med.Lab Tech. 37(2):29-31. I am proud to remember that I met Professor Pearse (God rest his soul) many years ago at an IAP meeting in Sydney - An icon (like Lee Luna, Russ Allison and Chuck Churukian) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, 19 April 2013 12:48 AM To: Geoff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Pituitary gland back in the days when I worked exclusively in neuropathology in Glasgow, the tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange fuchsin green) methods. I don't have the references to hand , although I believe the first was attributed to Tony Pearse, but can probably pull up the methods if needed Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Thursday, April 18, 2013 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Pituitary gland In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references that might be useful: > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > Read More: > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bustamante, Lin > Sent: Thursday, 18 April 2013 1:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pituitary gland > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > Thank you. > Lin. > > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mbmphoto <@t> gmail.com Thu Apr 18 19:10:47 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Apr 18 19:10:54 2013 Subject: [Histonet] Isopentane storage-the polling is open In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF079621@ex07.net.ucsf.edu> <66D57C82FCCC48F8B38FAE91D3F39554@HP2010> Message-ID: <5D1B41E9-9905-4EAB-A5F0-52E9C69113E8@gmail.com> Melanie, Who's your vendor for buying Novec 7000? Maria Mejia San Francisco, CA Affymetrix On Apr 17, 2013, at 10:08 AM, mwhite@mcleodhealth.org wrote: > I just wanted to clarify that we don't use Novec 7000 for freezing of > muscle tissue. We use it for other routine frozen sections on > humans.....Sorry if that caused any confusion. Thanks Tim for pointing that > out! > > > > > Melanie S. White, MT(ASCP) > Laboratory Supervisor, Systems/Anatomic Pathology > McLeod Regional Medical Center > (843) 777-2072 > > > > NOTICE: This e-mail message and all attachments transmitted with > it may contain legally PRIVILEGED and CONFIDENTIAL information > intended solely for the use of the addressee. If the reader of this > message is not the intended recipient, you are hereby notified > that any reading, dissemination, distribution, copying, or other use > of this message or its attachments is strictly prohibited. If you > have received this message in error, please notify the sender > immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Apr 18 19:16:22 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Apr 18 19:16:42 2013 Subject: [Histonet] RE: Calcium Stains? In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D24213D@xmdb04.nch.kids> Emily, I would suggest the alizarin Red S Technique. Since silver substitutes calcium in the Von Kossa's technique, it can therefore be regarded as a histochemical test for inorganic phosphates rather than calcium (see Meloan & Puchtler (1985) J Histotechnol 8(1):11-13). Alizarin Red S for Calcium allows the direct demonstration of insoluble calcium cations where Alizarin Red S forms an insoluble orange red lake. The technique is made specific by pH control (Garvey et al 1989, J Histotechnol 12 (3):225-227). Alizarin Red S also forms lakes with other metals such as barium, aluminium, mercury and magnesium. Fortunately, the bright red colouration of calcium can be easily differentiated from the dark red of other metal-lakes. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily Sent: Friday, 19 April 2013 3:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Calcium Stains? We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From ibernard <@t> uab.edu Fri Apr 19 05:31:26 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Fri Apr 19 05:32:50 2013 Subject: [Histonet] 2013 CAP Inspection Success & National Lab Message-ID: Colleagues, thank you for your input with questions. Your input and our implementation have proved successful. Hard work towards alignment of policies, procedures and practices to standards (CAP) all proved successful. Thank you to my staff, in particular SRA George Williams, for their hard work in preparation. Yesterday, 18th April 13, we were inspected by CAP and we are glad to report that we had a very successful outcome: Our Anatomic Pathology department scored with: "Zero Discrepancies and Recommendations". A very detailed inspection, in all aspects of our entire laboratory with 10 inspectors. Bottom-line: Our Lab is reaccredited another two years to serve our patient population. Also glad to report that prior to the inspection that our lab was selected as the 2013 Advance Magazine National Lab of the Year! Please see the April edition of Advance Magazine. Fellow Colorado Histo colleagues, I'll see you tomorrow for the Colorado Society of Histotechnology Meeting in Fort Collins. V/r Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP)- (Working on HTL) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 From Valerie.Hannen <@t> parrishmed.com Fri Apr 19 09:43:05 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Apr 19 09:43:10 2013 Subject: [Histonet] FNA preparation Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C2A@isexstore03> Good Morning fellow Histonetters!! I am hoping someone can answer this question for me. Is a General Lab Tech (working as an assistant) permitted to prepare FNA slides at the bedside, as well as stain them using the Wright Stain before the slides being to the Pathologist for interpretation? My thinking on it is that I believe that they should be able to because after all they make CBC slides and stain those. We are having a debate in our department and I would like to know what the correct answer is before I would implement any training for the General tech. Thanks ever so much!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From vrivera <@t> westderm.com Fri Apr 19 11:54:37 2013 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Fri Apr 19 11:54:45 2013 Subject: [Histonet] IHC Technician job description Message-ID: <3D4A471B82E7A44C87F6839732320D9F017C72@VSPDMS-ITEXMB02.DMS.COM> Hello fellow Histonetters: Does anyone have an IHC technician / specialist job description that they would wish to share with me? I am in the process of creating a new "IHC tech" specific position, so any help is much appreciated. Thank you, Vincent Rivera, HT (ASCP) QIHC Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) From twebster <@t> CRH.org Fri Apr 19 12:15:24 2013 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Fri Apr 19 12:15:35 2013 Subject: [Histonet] FNA preparation Message-ID: <7207186ED68FB542803CAF1CE6E82FF8055771@exmb1.crh.org> It should be fine for a general lab tech to prepare and stain FNA slides CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From craigak12 <@t> gmail.com Fri Apr 19 12:38:33 2013 From: craigak12 <@t> gmail.com (Jb) Date: Fri Apr 19 12:38:40 2013 Subject: [Histonet] Pen-Fix: Message-ID: Does anyone make their own version of pen-fix? It is a alcoholic formalin fixative. Can someone give the recipe? Sent from my iPhone From garret.t.miyamoto <@t> us.army.mil Fri Apr 19 16:02:16 2013 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Fri Apr 19 16:02:32 2013 Subject: [Histonet] Re: VCP-2001 cassette printer ink (UNCLASSIFIED) In-Reply-To: <76e0cb4e6ae89.5171b032@us.army.mil> References: <0MLI00JRMI5V8O90@mail14.int.ps1.us.army.mil> <76e0cb4e6ae89.5171b032@us.army.mil> Message-ID: <7740b2196a0ba.51712438@us.army.mil> UNCLASSIFIED Wayne, We have the VCP-3001 cassette printer and we buy the printer ribbon from Leica (800-225-3035). The stock number is V0590001F and it cost us $83.35 per ribbon which we bought several years ago. I don't know if it is the same one for your VCP-2001 but I'm sure when you call them, they can help you. Garret Miyamoto Tripler Army Medical Center Anatomic Pathology Dept., Histology Section On 04/19/13, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Fwd: [Histonet] AutoTech Embedder( Sakura) (Kiranjit Grewal) > 2. New Lab In KY (Hale, Meredith) > 3. email (McKenzie, Emily) > 4. Calcium Stains? (McKenzie, Emily) > 5. RE: Calcium Stains? (Roberta Horner) > 6. Biosafety in the Mohs Laboratory (Zimmerman, Billie) > 7. looking for control block of "Burkitt Lymphoma" (Hammel, Vicky) > 8. IHC question (Shirley A. Powell) > 9. VCP-2001 cassette printer question about ink (Wayne D Gill) > 10. atlanta employment (Michael Bourgeois) > 11. RE: RE: Pituitary gland (Tony Henwood (SCHN)) > 12. RE: RE: Pituitary gland (Tony Henwood (SCHN)) > 13. Re: Isopentane storage-the polling is open (Maria Mejia) > 14. RE: Calcium Stains? (Tony Henwood (SCHN)) > 15. 2013 CAP Inspection Success & National Lab (Ian R Bernard) > 16. FNA preparation (Hannen, Valerie) > 17. IHC Technician job description (Vincent Rivera) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 18 Apr 2013 10:13:23 -0700 > From: Kiranjit Grewal > Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Hello, > > I really need some feedback on this before we invest in this technology. > > Thanks. > > Kiran > Sent from my iPhone > > Begin forwarded message: > > > From: Kiranjit Grewal > > Date: April 17, 2013, 2:25:36 PM PDT > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] AutoTech Embedder( Sakura) > > > > Hello Dear Colleagues, > > > > We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. > > > > Anyone currently using it and would like to share their experience is great. > > > > Thank you, > > > > -Kiran > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 18 Apr 2013 17:18:02 +0000 > From: "Hale, Meredith" > Subject: [Histonet] New Lab In KY > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <0E828EC51C7CC445A51E53F81B64E8C72242CB@s-irv-exchmb.PathologyPartners.intranet> > > Content-Type: text/plain; charset="us-ascii" > > Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) > > * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens > > * Supervisor experience preferred > > * HT/HTL ASCP Certified > > * Experience with CLIA and CAP > > * Experience writing and maintaining policies and procedures > > * Prior laboratory start up experience is preferred > > * Ability to work independently > Duties include: > > * Grossing > > * Embedding > > * Microtomy > > * Staining; routine and special stains only > > * Maintain supply orders and laboratory budget > > * Ability to be flexible and take on additional duties' as needed > > * Ability to work independently > > * Maintenance of laboratory for inspections > > * Maintenance of quality records > > > > > > > Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com > > > Meredith Hale HT (ASCP)cm > Director External Sales Support > > Miraca Life Sciences > 6655 North MacArthur Blvd. > Irving , Texas 75039 > Office: 214-596-2219 > Cell: 469-648-8253 > Fax: 1-866-688-3280 > mhale@miracals.com> > > > > ------------------------------ > > Message: 3 > Date: Thu, 18 Apr 2013 12:36:27 -0500 > From: "McKenzie, Emily" > Subject: [Histonet] email > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Mckenzie.emily@mshil.com > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 4 > Date: Thu, 18 Apr 2013 12:42:37 -0500 > From: "McKenzie, Emily" > Subject: [Histonet] Calcium Stains? > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 5 > Date: Thu, 18 Apr 2013 14:15:15 -0400 > From: Roberta Horner > Subject: [Histonet] RE: Calcium Stains? > To: "McKenzie, Emily" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I use Dahl's Calcium stain > Roberta Horner HT/HTL > Penn State University > Animal Diagnostic Lab > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of McKenzie, Emily > Sent: Thursday, April 18, 2013 1:43 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Calcium Stains? > > We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Thu, 18 Apr 2013 18:32:30 +0000 > From: "Zimmerman, Billie" > Subject: [Histonet] Biosafety in the Mohs Laboratory > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7B3DEB32E69C034EACB479059C5DE3FF7673A7@EX-MLB-03.ad.georgiahealth.edu> > > Content-Type: text/plain; charset="us-ascii" > > After being well acquainted with the CAP question, in regards to decontamination of cryostats, I would like some feedback as to how some of you perform this function. > The lower cost cryostats do not contain a fumigation cycle but instruct the user to use alcohol and/or some type of germicidal spray for decontamination. I am hesitant about this and wondering if I'm just "old school" believing in formaldehyde fumigation for eliminating Hepatitis, HIV, and TB. In the cryostat manual, there is no mention of fumigation and the cleaner they suggest is not sold in the United States. Thanks in advance for your help! > Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. > > > ------------------------------ > > Message: 7 > Date: Thu, 18 Apr 2013 13:56:01 -0500 > From: "Hammel, Vicky" > Subject: [Histonet] looking for control block of "Burkitt Lymphoma" > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1E0E2B14C709174B8AC2BE0AE7F76833A2F2326ADB@EXCHANGE2K7.staprimecare.org> > > Content-Type: text/plain; charset="iso-8859-1" > > I am looking for a tissue block that contains "Burkitt Lymphoma", I have tissue with H.pylori, melanoma and amyloid that I would be willing to trade. Thank you, > > Vicky Hammel HTL, ASCP > Pathology Technical Consultant > > vhammel@primecare.org > > St. Alexius Medical Center > Histology Laboratory > 900 east Broadway > Bismarck, ND 58506 > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > > > ------------------------------ > > Message: 8 > Date: Thu, 18 Apr 2013 15:42:58 -0400 > From: "Shirley A. Powell" > Subject: [Histonet] IHC question > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <9BF995BC0E47744E9673A41486E24EE2579A9DDBFC@MERCERMAIL.MercerU.local> > Content-Type: text/plain; charset="us-ascii" > > I need to talk to any histotechs/researchers out there who are doing IHC on shellfish or fish. Please contact me personally at powell_sa@mercer.edu. Also any vendors who sell detection systems for them, please contact me. > > > Shirley A. Powell, HT(ASCP)HTL, QIHC > Technical Director > Histology Curricular Support Laboratory > Mercer University School of Medicine > 1550 College Street > Macon, GA 31207 > 478-301-2374 Lab > 478-301-5489 Fax > > > > ------------------------------ > > Message: 9 > Date: Thu, 18 Apr 2013 16:32:11 -0400 > From: Wayne D Gill > Subject: [Histonet] VCP-2001 cassette printer question about ink > To: histonet@lists.utsouthwestern.edu > Message-ID: <9765A008-719C-4B5A-955F-22D8013CC9BE@gmail.com> > Content-Type: text/plain; charset=us-ascii > > Does anyone know where to buy the ink ribbon for the VCP 2001 cassette printer? Vendor and order # would be helpful. Thank you. > > -Wayne > > > ------------------------------ > > Message: 10 > Date: Thu, 18 Apr 2013 17:11:50 -0400 > From: "Michael Bourgeois" > Subject: [Histonet] atlanta employment > To: > Message-ID: <006601ce3c79$58100f80$08302e80$@com> > Content-Type: text/plain; charset="us-ascii" > > Finan Templeton Dermatopathology Associates, is seeking an experienced, > intelligent, positive, dynamic and certified > histotechnician/histotechnologist to become a member of our extraordinary > team. The ideal candidate functions well in a team environment, has > exceptional histological skills, and possesses ASCP certification. > > We provide a full range of benefits for all full-time team members including > a generous profit sharing/retirement plan, paid vacation/sick time, and > medical, dental, and vision insurance plans. Part-time team members receive > partial benefit packages based upon hours worked. All Finan Templeton team > members receive generously competitive wages along with a commitment to an > environment of care, integrity, and mutual respect. > > > > Michael Bourgeois > > Lab Manager/Human Resources Manager > > (404)851-1766 | Fax (404)851-1767 | mdb@finantempleton.com > > 6095 Barfield Road Suite 200 | Atlanta, GA 30342 > > > > > > > > > > ------------------------------ > > Message: 11 > Date: Thu, 18 Apr 2013 22:54:19 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] RE: Pituitary gland > To: "'Geoff'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420E7@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Oh I am getting too old (and I was trying to be modern!) > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of Geoff > Sent: Friday, 19 April 2013 12:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: Pituitary gland > > In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. > > Geoff > > > On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > > Some references that might be useful: > > > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > > > > > Read More: > > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > > Laboratory Manager & Senior Scientist > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > > Westmead NSW 2145, AUSTRALIA > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of > > Bustamante, Lin > > Sent: Thursday, 18 April 2013 1:45 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Pituitary gland > > > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > > Thank you. > > Lin. > > > > > > Lin S. Bustamante, B.S., H.T.(ASCP) > > VIBS Histology Laboratory Supervisor > > College Of Veterinary Medicine > > Texas A&M University > > College Station, Texas 77843-4458 > > Phone: (979) 845-3177 > > Fax: (979) 458-3499 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************** > > *********** This email and any files transmitted with it are > > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > > ********************************************************************** > > *********** > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 12 > Date: Thu, 18 Apr 2013 23:25:52 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] RE: Pituitary gland > To: "'Houston, Ronald'" , > Geoff , "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420FD@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Yep, > > I missed one reference (by Prof Pearse) which I agree is my preferred technique: > > Everson Pearse, A. G. (1950). Differential stain for the human and animal anterior hypophysis. Biotechnic & Histochemistry, 25(2), 95-102. > > And where I used the stain: Henwood, A.F., (1983) "Crossreactivity in Immunoperoxidase: Human Placental Lactogen and the Pituitary". N.Z. J. Med.Lab Tech. 37(2):29-31. > > I am proud to remember that I met Professor Pearse (God rest his soul) many years ago at an IAP meeting in Sydney - An icon (like Lee Luna, Russ Allison and Chuck Churukian) > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of Houston, Ronald > Sent: Friday, 19 April 2013 12:48 AM > To: Geoff; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Pituitary gland > > back in the days when I worked exclusively in neuropathology in Glasgow, the tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange fuchsin green) methods. I don't have the references to hand , although I believe the first was attributed to Tony Pearse, but can probably pull up the methods if needed > > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of Geoff > Sent: Thursday, April 18, 2013 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: Pituitary gland > > In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. > > Geoff > > > On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > > Some references that might be useful: > > > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > > > > > Read More: > > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > > Laboratory Manager & Senior Scientist > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > > Westmead NSW 2145, AUSTRALIA > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of > > Bustamante, Lin > > Sent: Thursday, 18 April 2013 1:45 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Pituitary gland > > > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > > Thank you. > > Lin. > > > > > > Lin S. Bustamante, B.S., H.T.(ASCP) > > VIBS Histology Laboratory Supervisor > > College Of Veterinary Medicine > > Texas A&M University > > College Station, Texas 77843-4458 > > Phone: (979) 845-3177 > > Fax: (979) 458-3499 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************** > > *********** This email and any files transmitted with it are > > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > > ********************************************************************** > > *********** > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 13 > Date: Thu, 18 Apr 2013 17:10:47 -0700 > From: Maria Mejia > Subject: Re: [Histonet] Isopentane storage-the polling is open > To: mwhite@mcleodhealth.org > Cc: HistoNet , > histonet-bounces@lists.utsouthwestern.edu, "Morken, Timothy" > > Message-ID: <5D1B41E9-9905-4EAB-A5F0-52E9C69113E8@gmail.com> > Content-Type: text/plain; charset=us-ascii > > Melanie, > > Who's your vendor for buying Novec 7000? > > Maria Mejia > San Francisco, CA > Affymetrix > On Apr 17, 2013, at 10:08 AM, mwhite@mcleodhealth.org wrote: > > > I just wanted to clarify that we don't use Novec 7000 for freezing of > > muscle tissue. We use it for other routine frozen sections on > > humans.....Sorry if that caused any confusion. Thanks Tim for pointing that > > out! > > > > > > > > > > Melanie S. White, MT(ASCP) > > Laboratory Supervisor, Systems/Anatomic Pathology > > McLeod Regional Medical Center > > (843) 777-2072 > > > > > > > > NOTICE: This e-mail message and all attachments transmitted with > > it may contain legally PRIVILEGED and CONFIDENTIAL information > > intended solely for the use of the addressee. If the reader of this > > message is not the intended recipient, you are hereby notified > > that any reading, dissemination, distribution, copying, or other use > > of this message or its attachments is strictly prohibited. If you > > have received this message in error, please notify the sender > > immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Fri, 19 Apr 2013 00:16:22 +0000 > From: "Tony Henwood (SCHN)" > Subject: [Histonet] RE: Calcium Stains? > To: "'McKenzie, Emily'" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71579D24213D@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Emily, > > I would suggest the alizarin Red S Technique. > Since silver substitutes calcium in the Von Kossa's technique, it can therefore be regarded as a histochemical test for inorganic phosphates rather than calcium (see Meloan & Puchtler (1985) J Histotechnol 8(1):11-13). > > Alizarin Red S for Calcium allows the direct demonstration of insoluble calcium cations where Alizarin Red S forms an insoluble orange red lake. The technique is made specific by pH control (Garvey et al 1989, J Histotechnol 12 (3):225-227). Alizarin Red S also forms lakes with other metals such as barium, aluminium, mercury and magnesium. Fortunately, the bright red colouration of calcium can be easily differentiated from the dark red of other metal-lakes. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu](javascript:main.compose() On Behalf Of McKenzie, Emily > Sent: Friday, 19 April 2013 3:43 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Calcium Stains? > > We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 15 > Date: Fri, 19 Apr 2013 10:31:26 +0000 > From: Ian R Bernard > Subject: [Histonet] 2013 CAP Inspection Success & National Lab > To: "histonet@lists.utsouthwestern.edu" > > Cc: "BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Colleagues, thank you for your input with questions. Your input and our implementation have proved successful. > > Hard work towards alignment of policies, procedures and practices to standards (CAP) all proved successful. Thank you to my staff, in particular SRA George Williams, for their hard work in preparation. > > Yesterday, 18th April 13, we were inspected by CAP and we are glad to report that we had a very successful outcome: Our Anatomic Pathology department scored with: "Zero Discrepancies and Recommendations". > > A very detailed inspection, in all aspects of our entire laboratory with 10 inspectors. Bottom-line: Our Lab is reaccredited another two years to serve our patient population. > > Also glad to report that prior to the inspection that our lab was selected as the 2013 Advance Magazine National Lab of the Year! Please see the April edition of Advance Magazine. > > Fellow Colorado Histo colleagues, I'll see you tomorrow for the Colorado Society of Histotechnology Meeting in Fort Collins. > > V/r > Ian R. Bernard > Ian R. Bernard, MSHA, HT (ASCP)- (Working on HTL) > NCOIC-Manager, Anatomic Pathology Lab > 10th Medical Group > USAF Academy, CO 80840 > Graduate Certificate In Gerontology Student-UAB > 210-687-7540 > > > > ------------------------------ > > Message: 16 > Date: Fri, 19 Apr 2013 10:43:05 -0400 > From: "Hannen, Valerie" > Subject: [Histonet] FNA preparation > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C2A@isexstore03> > Content-Type: text/plain; charset="us-ascii" > > Good Morning fellow Histonetters!! > > I am hoping someone can answer this question for me. Is a General Lab Tech (working as an assistant) permitted to prepare FNA slides at the bedside, as well as > > stain them using the Wright Stain before the slides being to the Pathologist for interpretation? > > My thinking on it is that I believe that they should be able to because after all they make CBC slides and stain those. > > We are having a debate in our department and I would like to know what the correct answer is before I would implement any training for the General tech. > > Thanks ever so much!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > =================== > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > =================== > > ------------------------------ > > Message: 17 > Date: Fri, 19 Apr 2013 16:54:37 +0000 > From: Vincent Rivera > Subject: [Histonet] IHC Technician job description > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <3D4A471B82E7A44C87F6839732320D9F017C72@VSPDMS-ITEXMB02.DMS.COM> > Content-Type: text/plain; charset="us-ascii" > > Hello fellow Histonetters: > > Does anyone have an IHC technician / specialist job description that they would wish to share with me? I am in the process of creating a new "IHC tech" specific position, so any help is much appreciated. > > Thank you, > > Vincent Rivera, HT (ASCP) QIHC > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 113, Issue 20 > ***************************************** UNCLASSIFIED From carl.hobbs <@t> kcl.ac.uk Sat Apr 20 12:52:29 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Apr 20 12:52:39 2013 Subject: [Histonet] Pen-Fix Message-ID: <00D6B8253EAED840B8D04E235497381822DAC205@AM2PRD0311MB399.eurprd03.prod.outlook.com> If you want this recipe for fixing tissues for FFPWS, then performing Immuno: doesn't work +/- any AR, for me. Nice for morphology but, no better than many other precipitant+additive fixing fluids. Imho, it's like many other "fixes" that don't really work in a BIG way. Well, imho, if they all did...we would all be using them? Well, we all use HIER... THAT was a Paradigm shift! NB: a fixative is a chemical: dissolved in a solvent, it is then called a Fixing fluid. Pedantically, Carl Sent from my PC ;-) From histotalk <@t> yahoo.com Sat Apr 20 19:02:21 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Apr 20 19:02:28 2013 Subject: [Histonet] Re: Home based slide interpretation In-Reply-To: References: <1366205716.69850.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <1366502541.54048.YahooMailNeo@web121502.mail.ne1.yahoo.com> Correct. That is how it's done. ? Yours, Dave ________________________________ From: Debra Siena To: Rene J Buesa ; John Spaull Cc: Histonet Sent: Wednesday, April 17, 2013 10:41 AM Subject: RE: [Histonet] Re: Home based slide interpretation It is my understanding that whenever the reading of the slides is not performed at the testing site that they need to list that on their report and also they will need to have a CLIA license for their home or office that is offsite. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 8:35 AM To: John Spaull; JMacDonald@mtsac.edu Cc: Histonet Subject: [Histonet] Re: Home based slide interpretation John: You are absolutely right! That is also an issue. Its seems that with every bit of additional information the case about being illegal for a pathologist to read slides from home is building up more and more strong! Very interesting. Ren? J. From: John Spaull To: "rjbuesa@yahoo.com" ; "JMacDonald@mtsac.edu" Sent: Wednesday, April 17, 2013 2:44 AM Subject: Home based slide interpretation The UK Human Tissues Act (2006) is also relevant I think. To work with human tissue premises have to be registered. All tissue is tracked. The Health Service may have exemptions but generally tracking requires approved couriers etc. Registration of premises does require payment of a fee. ? One can imagine the headlines if your home working pathologist was transporting slides themselves and say left diagnostic samples on the train. Patient confidentiality around physical notes would also be something to be considered I guess. ? ------------------------------ ? Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald , ????? "histonet@lists.utsouthwestern.edu" ????? Message-ID: ????? <1366119506.58355.YahooMailNeo@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. ? From: Jennifer MacDonald To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation ? ? Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? Regards, ? John. ? John Spaull Histology Group, Molecular and Cellular Technologies, Platform Technology and Science, GlaxoSmithKline R&D, Stevenage, SG1 2NY, UK +44 (0)1438 763296 E-mail john.r.spaull@gsk.com ? ? This e-mail was sent by GlaxoSmithKline Services Unlimited (registered in England and Wales No. 1047315), which is a member of the GlaxoSmithKline group of companies. The registered address of GlaxoSmithKline Services Unlimited is 980 Great West Road, Brentford, Middlesex TW8 9GS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lins0701 <@t> gmail.com Sun Apr 21 15:19:55 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Sun Apr 21 15:20:00 2013 Subject: [Histonet] Per Diem position Message-ID: I'm looking for a per diem HT in the Temecula area (of CA), with experience who is able to be on-call for emergencies, sick leave, vacation, and miscellaneous. Must be experienced with embedding, sectioning, and special stains (Giemsa, Tri, PAS, Alcian Blue). Serious inquiries only please. Please send resume, references, and cover letter. Thanks, Sophia From meljdelbarrio <@t> yahoo.com Sun Apr 21 23:48:38 2013 From: meljdelbarrio <@t> yahoo.com (Mel John del Barrio) Date: Sun Apr 21 23:48:44 2013 Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Message-ID: <1366606118.4830.YahooMailNeo@web160905.mail.bf1.yahoo.com> Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com From CDavis <@t> che-east.org Mon Apr 22 09:56:22 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Mon Apr 22 09:58:07 2013 Subject: [Histonet] RE: IHC Technician job description In-Reply-To: References: Message-ID: <08861B9CF6C7774E874635A4818AE37B07A3C6D9@CHEXCMS01.one.ads.che.org> Vincent, you might want check out CLIA 88' complex testing requirement before you write that job description. Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, April 19, 2013 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fwd: [Histonet] AutoTech Embedder( Sakura) (Kiranjit Grewal) 2. New Lab In KY (Hale, Meredith) 3. email (McKenzie, Emily) 4. Calcium Stains? (McKenzie, Emily) 5. RE: Calcium Stains? (Roberta Horner) 6. Biosafety in the Mohs Laboratory (Zimmerman, Billie) 7. looking for control block of "Burkitt Lymphoma" (Hammel, Vicky) 8. IHC question (Shirley A. Powell) 9. VCP-2001 cassette printer question about ink (Wayne D Gill) 10. atlanta employment (Michael Bourgeois) 11. RE: RE: Pituitary gland (Tony Henwood (SCHN)) 12. RE: RE: Pituitary gland (Tony Henwood (SCHN)) 13. Re: Isopentane storage-the polling is open (Maria Mejia) 14. RE: Calcium Stains? (Tony Henwood (SCHN)) 15. 2013 CAP Inspection Success & National Lab (Ian R Bernard) 16. FNA preparation (Hannen, Valerie) 17. IHC Technician job description (Vincent Rivera) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 Apr 2013 10:13:23 -0700 From: Kiranjit Grewal Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: > From: Kiranjit Grewal > Date: April 17, 2013, 2:25:36 PM PDT > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AutoTech Embedder( Sakura) > > Hello Dear Colleagues, > > We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. > > Anyone currently using it and would like to share their experience is great. > > Thank you, > > -Kiran > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 18 Apr 2013 17:18:02 +0000 From: "Hale, Meredith" Subject: [Histonet] New Lab In KY To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0E828EC51C7CC445A51E53F81B64E8C72242CB@s-irv-exchmb.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> ------------------------------ Message: 3 Date: Thu, 18 Apr 2013 12:36:27 -0500 From: "McKenzie, Emily" Subject: [Histonet] email To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Mckenzie.emily@mshil.com ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 4 Date: Thu, 18 Apr 2013 12:42:37 -0500 From: "McKenzie, Emily" Subject: [Histonet] Calcium Stains? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 5 Date: Thu, 18 Apr 2013 14:15:15 -0400 From: Roberta Horner Subject: [Histonet] RE: Calcium Stains? To: "McKenzie, Emily" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I use Dahl's Calcium stain Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily Sent: Thursday, April 18, 2013 1:43 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Calcium Stains? We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 18 Apr 2013 18:32:30 +0000 From: "Zimmerman, Billie" Subject: [Histonet] Biosafety in the Mohs Laboratory To: "histonet@lists.utsouthwestern.edu" Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF7673A7@EX-MLB-03.ad.georgiahealth.edu> Content-Type: text/plain; charset="us-ascii" After being well acquainted with the CAP question, in regards to decontamination of cryostats, I would like some feedback as to how some of you perform this function. The lower cost cryostats do not contain a fumigation cycle but instruct the user to use alcohol and/or some type of germicidal spray for decontamination. I am hesitant about this and wondering if I'm just "old school" believing in formaldehyde fumigation for eliminating Hepatitis, HIV, and TB. In the cryostat manual, there is no mention of fumigation and the cleaner they suggest is not sold in the United States. Thanks in advance for your help! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. ------------------------------ Message: 7 Date: Thu, 18 Apr 2013 13:56:01 -0500 From: "Hammel, Vicky" Subject: [Histonet] looking for control block of "Burkitt Lymphoma" To: "histonet@lists.utsouthwestern.edu" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2F2326ADB@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="iso-8859-1" I am looking for a tissue block that contains "Burkitt Lymphoma", I have tissue with H.pylori, melanoma and amyloid that I would be willing to trade. Thank you, Vicky Hammel HTL, ASCP Pathology Technical Consultant vhammel@primecare.org St. Alexius Medical Center Histology Laboratory 900 east Broadway Bismarck, ND 58506 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------ Message: 8 Date: Thu, 18 Apr 2013 15:42:58 -0400 From: "Shirley A. Powell" Subject: [Histonet] IHC question To: "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE2579A9DDBFC@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" I need to talk to any histotechs/researchers out there who are doing IHC on shellfish or fish. Please contact me personally at powell_sa@mercer.edu. Also any vendors who sell detection systems for them, please contact me. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ------------------------------ Message: 9 Date: Thu, 18 Apr 2013 16:32:11 -0400 From: Wayne D Gill Subject: [Histonet] VCP-2001 cassette printer question about ink To: histonet@lists.utsouthwestern.edu Message-ID: <9765A008-719C-4B5A-955F-22D8013CC9BE@gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone know where to buy the ink ribbon for the VCP 2001 cassette printer? Vendor and order # would be helpful. Thank you. -Wayne ------------------------------ Message: 10 Date: Thu, 18 Apr 2013 17:11:50 -0400 From: "Michael Bourgeois" Subject: [Histonet] atlanta employment To: Message-ID: <006601ce3c79$58100f80$08302e80$@com> Content-Type: text/plain; charset="us-ascii" Finan Templeton Dermatopathology Associates, is seeking an experienced, intelligent, positive, dynamic and certified histotechnician/histotechnologist to become a member of our extraordinary team. The ideal candidate functions well in a team environment, has exceptional histological skills, and possesses ASCP certification. We provide a full range of benefits for all full-time team members including a generous profit sharing/retirement plan, paid vacation/sick time, and medical, dental, and vision insurance plans. Part-time team members receive partial benefit packages based upon hours worked. All Finan Templeton team members receive generously competitive wages along with a commitment to an environment of care, integrity, and mutual respect. Michael Bourgeois Lab Manager/Human Resources Manager (404)851-1766 | Fax (404)851-1767 | mdb@finantempleton.com 6095 Barfield Road Suite 200 | Atlanta, GA 30342 ------------------------------ Message: 11 Date: Thu, 18 Apr 2013 22:54:19 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] RE: Pituitary gland To: "'Geoff'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420E7@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Oh I am getting too old (and I was trying to be modern!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Friday, 19 April 2013 12:29 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Pituitary gland In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references that might be useful: > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > Read More: > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bustamante, Lin > Sent: Thursday, 18 April 2013 1:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pituitary gland > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > Thank you. > Lin. > > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 12 Date: Thu, 18 Apr 2013 23:25:52 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] RE: Pituitary gland To: "'Houston, Ronald'" , Geoff , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420FD@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Yep, I missed one reference (by Prof Pearse) which I agree is my preferred technique: Everson Pearse, A. G. (1950). Differential stain for the human and animal anterior hypophysis. Biotechnic & Histochemistry, 25(2), 95-102. And where I used the stain: Henwood, A.F., (1983) "Crossreactivity in Immunoperoxidase: Human Placental Lactogen and the Pituitary". N.Z. J. Med.Lab Tech. 37(2):29-31. I am proud to remember that I met Professor Pearse (God rest his soul) many years ago at an IAP meeting in Sydney - An icon (like Lee Luna, Russ Allison and Chuck Churukian) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, 19 April 2013 12:48 AM To: Geoff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Pituitary gland back in the days when I worked exclusively in neuropathology in Glasgow, the tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange fuchsin green) methods. I don't have the references to hand , although I believe the first was attributed to Tony Pearse, but can probably pull up the methods if needed Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Thursday, April 18, 2013 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Pituitary gland In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references that might be useful: > > Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122. > > Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. > > Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346. > > > Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. > > > > Read More: > http://informahealthcare.com/doi/abs/10.3109/10520295909114648 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bustamante, Lin > Sent: Thursday, 18 April 2013 1:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pituitary gland > > Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. > Thank you. > Lin. > > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 13 Date: Thu, 18 Apr 2013 17:10:47 -0700 From: Maria Mejia Subject: Re: [Histonet] Isopentane storage-the polling is open To: mwhite@mcleodhealth.org Cc: HistoNet , histonet-bounces@lists.utsouthwestern.edu, "Morken, Timothy" Message-ID: <5D1B41E9-9905-4EAB-A5F0-52E9C69113E8@gmail.com> Content-Type: text/plain; charset=us-ascii Melanie, Who's your vendor for buying Novec 7000? Maria Mejia San Francisco, CA Affymetrix On Apr 17, 2013, at 10:08 AM, mwhite@mcleodhealth.org wrote: > I just wanted to clarify that we don't use Novec 7000 for freezing of > muscle tissue. We use it for other routine frozen sections on > humans.....Sorry if that caused any confusion. Thanks Tim for pointing that > out! > > > > > Melanie S. White, MT(ASCP) > Laboratory Supervisor, Systems/Anatomic Pathology > McLeod Regional Medical Center > (843) 777-2072 > > > > NOTICE: This e-mail message and all attachments transmitted with > it may contain legally PRIVILEGED and CONFIDENTIAL information > intended solely for the use of the addressee. If the reader of this > message is not the intended recipient, you are hereby notified > that any reading, dissemination, distribution, copying, or other use > of this message or its attachments is strictly prohibited. If you > have received this message in error, please notify the sender > immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 19 Apr 2013 00:16:22 +0000 From: "Tony Henwood (SCHN)" Subject: [Histonet] RE: Calcium Stains? To: "'McKenzie, Emily'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <6D6BD1DE8A5571489398B392A38A71579D24213D@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Emily, I would suggest the alizarin Red S Technique. Since silver substitutes calcium in the Von Kossa's technique, it can therefore be regarded as a histochemical test for inorganic phosphates rather than calcium (see Meloan & Puchtler (1985) J Histotechnol 8(1):11-13). Alizarin Red S for Calcium allows the direct demonstration of insoluble calcium cations where Alizarin Red S forms an insoluble orange red lake. The technique is made specific by pH control (Garvey et al 1989, J Histotechnol 12 (3):225-227). Alizarin Red S also forms lakes with other metals such as barium, aluminium, mercury and magnesium. Fortunately, the bright red colouration of calcium can be easily differentiated from the dark red of other metal-lakes. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily Sent: Friday, 19 April 2013 3:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Calcium Stains? We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue? ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 15 Date: Fri, 19 Apr 2013 10:31:26 +0000 From: Ian R Bernard Subject: [Histonet] 2013 CAP Inspection Success & National Lab To: "histonet@lists.utsouthwestern.edu" Cc: "BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH" Message-ID: Content-Type: text/plain; charset="us-ascii" Colleagues, thank you for your input with questions. Your input and our implementation have proved successful. Hard work towards alignment of policies, procedures and practices to standards (CAP) all proved successful. Thank you to my staff, in particular SRA George Williams, for their hard work in preparation. Yesterday, 18th April 13, we were inspected by CAP and we are glad to report that we had a very successful outcome: Our Anatomic Pathology department scored with: "Zero Discrepancies and Recommendations". A very detailed inspection, in all aspects of our entire laboratory with 10 inspectors. Bottom-line: Our Lab is reaccredited another two years to serve our patient population. Also glad to report that prior to the inspection that our lab was selected as the 2013 Advance Magazine National Lab of the Year! Please see the April edition of Advance Magazine. Fellow Colorado Histo colleagues, I'll see you tomorrow for the Colorado Society of Histotechnology Meeting in Fort Collins. V/r Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP)- (Working on HTL) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 ------------------------------ Message: 16 Date: Fri, 19 Apr 2013 10:43:05 -0400 From: "Hannen, Valerie" Subject: [Histonet] FNA preparation To: "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C2A@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning fellow Histonetters!! I am hoping someone can answer this question for me. Is a General Lab Tech (working as an assistant) permitted to prepare FNA slides at the bedside, as well as stain them using the Wright Stain before the slides being to the Pathologist for interpretation? My thinking on it is that I believe that they should be able to because after all they make CBC slides and stain those. We are having a debate in our department and I would like to know what the correct answer is before I would implement any training for the General tech. Thanks ever so much!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 17 Date: Fri, 19 Apr 2013 16:54:37 +0000 From: Vincent Rivera Subject: [Histonet] IHC Technician job description To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3D4A471B82E7A44C87F6839732320D9F017C72@VSPDMS-ITEXMB02.DMS.COM> Content-Type: text/plain; charset="us-ascii" Hello fellow Histonetters: Does anyone have an IHC technician / specialist job description that they would wish to share with me? I am in the process of creating a new "IHC tech" specific position, so any help is much appreciated. Thank you, Vincent Rivera, HT (ASCP) QIHC Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 20 ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Pat.Bell <@t> ucdenver.edu Mon Apr 22 11:45:31 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Mon Apr 22 11:45:35 2013 Subject: [Histonet] COX2 in Human Message-ID: <64DB27005E2FD3439E88502D7A5C91210101FE90455F@CORTEZ.ucdenver.pvt> Hello to all! I have been doing COX2 in human breast tissue and normally it works great. Lately, however, I have been getting a lot of background and nonspecific staining even with a new lot. Does anyone might know what I am doing wrong and how I could correct this problem? Thank you and HAPPY LAB WEEK!!! Pat Pat Bell (HT-ASCP) University of Colorado, Denver Medical Oncology 12801 E 17th Ave, MS 8117 Aurora, Colorado 80045 303-724-6077 pat.bell@ucdenver.edu From relia1 <@t> earthlink.net Mon Apr 22 11:49:07 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Apr 22 11:49:12 2013 Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! Message-ID: <000001ce3f79$4ebe7360$ec3b5a20$@earthlink.net> Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldn?t be an email from me if I didn?t tell you about my current openings. Please take a second and check them out Here Are My Spotlight Opportunities: Night Shift Histotech ? Patterson, NJ Brand New Lab!! Day Shift Histotech ? Salem, VA beautiful area and great Team HT/HTL Mohs Histotech ? Long Beach, CA ? Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net?Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ibernard <@t> uab.edu Mon Apr 22 12:22:13 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Mon Apr 22 12:22:21 2013 Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! In-Reply-To: <000001ce3f79$4ebe7360$ec3b5a20$@earthlink.net> References: <000001ce3f79$4ebe7360$ec3b5a20$@earthlink.net> Message-ID: - We are celebrating with sponsor breakfasts and lunches for lab personnel from local restaurants each day. - Different internal games. - Educational Lab Week information and mementos for patients and staff alike - And finally, two half days this week, some personnel will go hiking and others on another day horseback riding. That way our mission is staffed without negative impact to patient care V/r Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Monday, April 22, 2013 11:49 AM To: Histonet Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question. How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldn't be an email from me if I didn't tell you about my current openings. Please take a second and check them out. Here Are My Spotlight Opportunities: Night Shift Histotech - Patterson, NJ Brand New Lab!! Day Shift Histotech - Salem, VA beautiful area and great Team HT/HTL Mohs Histotech - Long Beach, CA - Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net?Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Apr 22 12:23:46 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Apr 22 12:23:53 2013 Subject: [Histonet] staining near pap pen only???? Message-ID: Hello Histonetters! We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as if the tray was crooked) but both ends and NOT the middle. Can anyone think of what would cause this? My boss came up with the idea that the solution is evaporating and therefore, it's more concentrated on the ends, but that seems a stretch. Then again, it's better than what I came up with, which is nothing! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From talulahgosh <@t> gmail.com Mon Apr 22 12:35:48 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Apr 22 12:35:56 2013 Subject: [Histonet] staining near pap pen only???? In-Reply-To: <761E2B5697F795489C8710BCC72141FF07AD9F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF07AD9F@ex07.net.ucsf.edu> Message-ID: Sorry, I forgot to mention, this is manual staining. Also, the area between the pap pen appears to be covered evenly with solution. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Apr 22, 2013 at 1:32 PM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > Emily, It could be that the reagent is pooling off the ends of the tissue. > Have you observed the tissue during staining to see what is happening to > the liquid on the slide? Is this manual or automated? > > I agree it is strange, because the usual artifact you see with the pap pen > is that the pen liquid covers part of the tissue and THAT part does not > stain. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Monday, April 22, 2013 10:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] staining near pap pen only???? > > Hello Histonetters! > > We have been doing triple staining on frozen tissue for a while. Just > recently it started to stain only near the pap pen (the sections have two > lines of pap pen, one where they begin and one where they end). This makes > no sense to me, as the staining isn't only on one end (as if the tray was > crooked) but both ends and NOT the middle. Can anyone think of what would > cause this? > My boss came up with the idea that the solution is evaporating and > therefore, it's more concentrated on the ends, but that seems a stretch. > Then again, it's better than what I came up with, which is nothing! > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SHUNTER <@t> beaumont.edu Mon Apr 22 14:29:56 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Mon Apr 22 14:30:04 2013 Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra In-Reply-To: <1366606118.4830.YahooMailNeo@web160905.mail.bf1.yahoo.com> References: <1366606118.4830.YahooMailNeo@web160905.mail.bf1.yahoo.com> Message-ID: We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Mon Apr 22 14:40:39 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Mon Apr 22 14:40:44 2013 Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra In-Reply-To: References: <1366606118.4830.YahooMailNeo@web160905.mail.bf1.yahoo.com> Message-ID: We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Mon Apr 22 15:22:29 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Mon Apr 22 15:22:39 2013 Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra In-Reply-To: <1786428909-1366661388-cardhu_decombobulator_blackberry.rim.net-947637074-@b14.c1.bise6.blackberry> References: <1366606118.4830.YahooMailNeo@web160905.mail.bf1.yahoo.com> <1786428909-1366661388-cardhu_decombobulator_blackberry.rim.net-947637074-@b14.c1.bise6.blackberry> Message-ID: Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips.... Vanessa -----Original Message----- From: elonergan@metrocast.net [mailto:elonergan@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by AT&T -----Original Message----- From: Vanessa Perez Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Tue Apr 23 06:49:39 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Apr 23 06:50:08 2013 Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A00274B62DBEF@CVM76.vetmed.wsu.edu> References: <1366606118.4830.YahooMailNeo@web160905.mail.bf1.yahoo.com> <1786428909-1366661388-cardhu_decombobulator_blackberry.rim.net-947637074-@b14.c1.bise6.blackberry> <9EF5279EBDFE6E4FB6605E8F183A00274B62DBEF@CVM76.vetmed.wsu.edu> Message-ID: You just need the FITC antibodies. No kit required. The protocol is under the procedure Fluorescence IHC Basically all the machine does is dispense the antibody on the slide for however long you choose. And some rinses of reaction buffer... Vanessa -----Original Message----- From: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Sent: Monday, April 22, 2013 3:52 PM To: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi Vanessa, I've been following this line of email, since I may need to start doing fluorescence on XT. Which kit/program/protocol do you use on the XT? Can you run them without a kit? Thankyou, Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, April 22, 2013 1:22 PM To: elonergan@metrocast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips.... Vanessa -----Original Message----- From: elonergan@metrocast.net [mailto:elonergan@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by AT&T -----Original Message----- From: Vanessa Perez Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandy.Cope-Yokoyama <@t> childrens.com Tue Apr 23 08:12:26 2013 From: Sandy.Cope-Yokoyama <@t> childrens.com (Sandy Cope-yokoyama) Date: Tue Apr 23 08:12:29 2013 Subject: [Histonet] Forwarded post for a histonet member: Subject: CLIA 24-hour gross review. Message-ID: <6F81318C8F5F984D972778B918A7D769310563@CMCPBEXMAIL12.Childrens.med> I am the sole histologist in a gastroenterology laboratory situated in a GI clinic. Complying with the 24-hour grossing review by the pathologist is a challenge because my slides travel by courier across town to the pathologist to read. I receive specimens throughout the day and perform grossing in the early AM then again at 11 AM. My pathologist signs copies of the requisition that I fax over that contains the gross and sends them back to me. On a daily basis this works just fine. Fridays and holidays are a pickle but we manage. My question is the intent of the 24-hour review. The inspector cannot tell me why this was put in place, what the pathologist is supposed to do with this information and/or how to tell if it is accurate because he does not receive the slides until the following day which would be outside of the 24-hour review rule. Can anyone tell the intent of the rule? How does this rule improve patient care? Even in a hospital laboratory, once a biopsy is grossed and placed in a cassette, how on earth does looking at the written gross of how big the specimen is or what color it is impact further actions that can take place? What remedial action does CLIA expect? Thanks for your input, Gastro Gal Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From Sherrian.McAnn <@t> va.gov Tue Apr 23 11:42:43 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Apr 23 11:43:43 2013 Subject: [Histonet] Blades Message-ID: <61E2B58CECEF384094A363989D47C09009BA28B7@VHAV17MSGA2.v17.med.va.gov> I have been in histology and cutting for about 26 years now. I have used many types of blades, high and low profile. My favorite and I think the best ones are Surgipath Teflon coated high or low profile blades. I believe the high profile blades are the best but I do realize that not everyone has that option. I think if you tried them you wouldn't be disappointed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Monday, April 15, 2013 12:39 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Tue Apr 23 11:50:27 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Apr 23 11:50:36 2013 Subject: [Histonet] New Lab Message-ID: <0E828EC51C7CC445A51E53F81B64E8C722D557@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From bakevictoria <@t> gmail.com Tue Apr 23 13:10:15 2013 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Apr 23 13:10:20 2013 Subject: [Histonet] Is the server down? Message-ID: Haven't seen any posts over past day or so. Vikki From ihcman2010 <@t> hotmail.com Tue Apr 23 13:15:32 2013 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Tue Apr 23 13:15:37 2013 Subject: [Histonet] Glen Message-ID: http://1000fungames.com/kyy.php?facebook=likeit&user=safari7.16&os=windows7&search=lifenews ihcman2010@hotmail.com Glen ************** May you be spared the indignities and infirmities of old age. . From akbitting <@t> geisinger.edu Tue Apr 23 13:50:26 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Apr 23 13:59:55 2013 Subject: [Histonet] Is the server down? In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F63A58304F@GHSEXMBX2W8K1V.geisinger.edu> Vikki, Didn't they tell you?? You were voted off the island. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, April 23, 2013 2:10 PM To: Histo Net list server Subject: [Histonet] Is the server down? Haven't seen any posts over past day or so. Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From pzeitlow <@t> bbpllab.com Tue Apr 23 14:33:49 2013 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Tue Apr 23 14:33:48 2013 Subject: [Histonet] Image analysis Message-ID: <6DB7235FE14F1E49ABC5794DF98C751F0A678DB748@exsrv07> Looking for opinions on digital image analyzers and software... want to do quantitative image analysis for ER/PR and HER2 IHC. Thanks! Greg Z From pzeitlow <@t> bbpllab.com Tue Apr 23 14:40:48 2013 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Tue Apr 23 14:40:46 2013 Subject: [Histonet] Recall: Image analysis Message-ID: <6DB7235FE14F1E49ABC5794DF98C751F0A678DB74C@exsrv07> Pat Zeitlow would like to recall the message, "Image analysis". From estellamireles <@t> gmail.com Tue Apr 23 15:02:36 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Apr 23 15:02:42 2013 Subject: [Histonet] Who is scanning thieir H&E's slides for Path. Message-ID: We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella From rob <@t> foliobio.com Tue Apr 23 15:12:08 2013 From: rob <@t> foliobio.com (Rob Day) Date: Tue Apr 23 15:12:50 2013 Subject: [Histonet] Who is scanning thieir H&E's slides for Path. In-Reply-To: References: Message-ID: We love Aperio. http://www.aperio.com On Apr 23, 2013, at 4:02 PM, Stella Mireles wrote: > We are gathering info. on the possibility of scanning our routine slides > for our Path. > If you are doing this in your lab, would you mind sharing some info. on the > eqiupment you are using? > > Stella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 skype: invasifspecies http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. From Ronald.Houston <@t> nationwidechildrens.org Tue Apr 23 15:17:38 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Apr 23 15:17:43 2013 Subject: [Histonet] Who is scanning thieir H&E's slides for Path. In-Reply-To: References: Message-ID: Aperio Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, April 23, 2013 4:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Who is scanning thieir H&E's slides for Path. We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Apr 23 15:26:24 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Apr 23 15:26:29 2013 Subject: [Histonet] Who is scanning thieir H&E's slides for Path. In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F093D1968CE@EXCHMB01.isis.local> Aperio. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 23, 2013 1:18 PM To: Stella Mireles; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who is scanning thieir H&E's slides for Path. Aperio Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, April 23, 2013 4:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Who is scanning thieir H&E's slides for Path. We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Apr 23 15:50:14 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 23 15:50:23 2013 Subject: [Histonet] RE: Image analysis In-Reply-To: <6DB7235FE14F1E49ABC5794DF98C751F0A678DB748@exsrv07> References: <6DB7235FE14F1E49ABC5794DF98C751F0A678DB748@exsrv07> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2B96@SBS2K8.premierlab.local> Pat There is a list of 510K cleared algorithms on the DPA website - Here is the link. https://digitalpathologyassociation.org/_data/files/DPA_Regulatory-FDA-510k_list.pdf Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Tuesday, April 23, 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Image analysis Looking for opinions on digital image analyzers and software... want to do quantitative image analysis for ER/PR and HER2 IHC. Thanks! Greg Z _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Apr 23 16:39:47 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 23 16:39:52 2013 Subject: [Histonet] RE: Image analysis In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2B96@SBS2K8.premierlab.local> References: <6DB7235FE14F1E49ABC5794DF98C751F0A678DB748@exsrv07> <14E2C6176416974295479C64A11CB9AE016411AC2B96@SBS2K8.premierlab.local> Message-ID: I'd like to add that we use the Aperio system with Ventana's antibody and it works well. It took a lot of work tweaking of the algorithm to get the software to score accurately using the 4B5 clone but that was the stain our pathologists were already used to. Mark On Tue, Apr 23, 2013 at 1:50 PM, Elizabeth Chlipala wrote: > Pat > > There is a list of 510K cleared algorithms on the DPA website - Here is > the link. > > > https://digitalpathologyassociation.org/_data/files/DPA_Regulatory-FDA-510k_list.pdf > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow > Sent: Tuesday, April 23, 2013 1:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Image analysis > > Looking for opinions on digital image analyzers and software... want to do > quantitative image analysis for ER/PR and HER2 IHC. > > > Thanks! > > Greg Z > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From litepath2000 <@t> yahoo.com Wed Apr 24 07:27:33 2013 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Wed Apr 24 07:27:38 2013 Subject: [Histonet] SUNY Cobleskill: Seeking Histotechnology Education Program Director Message-ID: <1366806453.69216.YahooMailNeo@web141205.mail.bf1.yahoo.com> Posting on behalf of the SUNY Cobleskill Histotechnology Program: SUNY Cobleskill is seeking talented and innovative individuals to join the college community as we build a new vision for our second century as a college of agriculture and technology. Offering associate's and bachelor's degree programs, SUNY Cobleskill values innovative teaching and faculty committed to engaging pedagogies, collaborative and interdisciplinary endeavors, global perspectives, applied learning including internships and undergraduate research, and high levels of interaction with students. The Natural Science Department and the Histotechnology Program is actively seeking a part-time adjunct for the role of Educational Coordinator for the Histotechnology Program. Job Description: This new position will work with the Histotechnician Program Director to maintain and enhance the academic and technical training of histotechnology students. Duties may include, but not be limited to, recruitment of students and new affiliate sites for internships; clinical coordination and site visits; classroom and laboratory teaching; collating and assessing data; and evaluating program effectiveness. Requirements: Bachelor's degree and three years of experience in Histotechnology required; master's degree preferred. The successful candidate will be a histotechnology professional who is certified by a national certifying agency. Excellent public relations, organization, decision making and creative skills are required. The candidate will have knowledge of educational methods and current accreditation and certification procedures. Additional Information: Half-time, 11-month position to serve as the Education Coordinator for the Histotechnician A.A.S. program; begins August 2013. This position offers full New York State benefits which are among the most comprehensive in the country. Salary Range: $20,000 ? $21,000. SUNY Cobleskill is committed to providing a safe and productive learning and living community for our students and employees. To achieve that goal, we conduct background investigations for all final candidates being considered for employment. Any offer of employment is contingent on the successful completion of the background check. Application Instructions: The application review process will begin immediately and will continue until a suitable candidate is found. Completed application consists of the following: 1. Letter of application, addressing qualifications, position responsibilities, and college values 2. Copies of official transcripts (originals required if hired) 3. Current CV 4. Names, emails and phone numbers of at least three professional references ? For more information and a links to the online application, please visit the SUNY Cobleskill website at: http://www.cobleskill.edu A link to the application page can also be found on the New York State Histotechnological Society website at: http://www.nyhisto.org/2013/04/suny-cobleskill-seeking-histotechnology-education-program-director/ ------- Luis Chiriboga Ph.D. President, New York State Histotechnological Society NYSHS Website: www.nyhisto.org NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. From LStadler <@t> cbiolabs.com Wed Apr 24 09:16:14 2013 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Wed Apr 24 09:17:35 2013 Subject: [Histonet] Blades Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC0176A6D10C@cbiolabs05.CBiolabs.local> I generally use CL Sturkey Extremus Low Profile Blades for pretty much everything...when I am having trouble with intestine sections, I use the DuraEdge Encore blade and it usually works very well. Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 23, 2013 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Happy Lab Week from Pam Barker and RELIA Solutions!! (Ian R Bernard) 2. staining near pap pen only???? (Emily Sours) 3. Re: staining near pap pen only???? (Emily Sours) 4. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Sue Hunter) 5. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 6. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 7. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 8. Forwarded post for a histonet member: Subject: CLIA 24-hour gross review. (Sandy Cope-yokoyama) 9. RE: Blades (McAnn, Sherrian) 10. New Lab (Hale, Meredith) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Apr 2013 17:22:13 +0000 From: Ian R Bernard Subject: RE: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! To: Pam Barker , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" - We are celebrating with sponsor breakfasts and lunches for lab personnel from local restaurants each day. - Different internal games. - Educational Lab Week information and mementos for patients and staff alike - And finally, two half days this week, some personnel will go hiking and others on another day horseback riding. That way our mission is staffed without negative impact to patient care V/r Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Monday, April 22, 2013 11:49 AM To: Histonet Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question. How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldn't be an email from me if I didn't tell you about my current openings. Please take a second and check them out. Here Are My Spotlight Opportunities: Night Shift Histotech - Patterson, NJ Brand New Lab!! Day Shift Histotech - Salem, VA beautiful area and great Team HT/HTL Mohs Histotech - Long Beach, CA - Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net?Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 22 Apr 2013 13:23:46 -0400 From: Emily Sours Subject: [Histonet] staining near pap pen only???? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello Histonetters! We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as if the tray was crooked) but both ends and NOT the middle. Can anyone think of what would cause this? My boss came up with the idea that the solution is evaporating and therefore, it's more concentrated on the ends, but that seems a stretch. Then again, it's better than what I came up with, which is nothing! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" ------------------------------ Message: 3 Date: Mon, 22 Apr 2013 13:35:48 -0400 From: Emily Sours Subject: Re: [Histonet] staining near pap pen only???? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Sorry, I forgot to mention, this is manual staining. Also, the area between the pap pen appears to be covered evenly with solution. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Apr 22, 2013 at 1:32 PM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > Emily, It could be that the reagent is pooling off the ends of the tissue. > Have you observed the tissue during staining to see what is happening > to the liquid on the slide? Is this manual or automated? > > I agree it is strange, because the usual artifact you see with the pap > pen is that the pen liquid covers part of the tissue and THAT part > does not stain. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Monday, April 22, 2013 10:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] staining near pap pen only???? > > Hello Histonetters! > > We have been doing triple staining on frozen tissue for a while. Just > recently it started to stain only near the pap pen (the sections have > two lines of pap pen, one where they begin and one where they end). > This makes no sense to me, as the staining isn't only on one end (as > if the tray was > crooked) but both ends and NOT the middle. Can anyone think of what > would cause this? > My boss came up with the idea that the solution is evaporating and > therefore, it's more concentrated on the ends, but that seems a stretch. > Then again, it's better than what I came up with, which is nothing! > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a > new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Mon, 22 Apr 2013 19:29:56 +0000 From: Sue Hunter Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: Mel John del Barrio , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 22 Apr 2013 14:40:39 -0500 From: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 22 Apr 2013 15:22:29 -0500 From: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: "elonergan@metrocast.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips.... Vanessa -----Original Message----- From: elonergan@metrocast.net [mailto:elonergan@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by AT&T -----Original Message----- From: Vanessa Perez Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 23 Apr 2013 06:49:39 -0500 From: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: "Truscott, Tom" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" You just need the FITC antibodies. No kit required. The protocol is under the procedure Fluorescence IHC Basically all the machine does is dispense the antibody on the slide for however long you choose. And some rinses of reaction buffer... Vanessa -----Original Message----- From: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Sent: Monday, April 22, 2013 3:52 PM To: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi Vanessa, I've been following this line of email, since I may need to start doing fluorescence on XT. Which kit/program/protocol do you use on the XT? Can you run them without a kit? Thankyou, Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, April 22, 2013 1:22 PM To: elonergan@metrocast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips.... Vanessa -----Original Message----- From: elonergan@metrocast.net [mailto:elonergan@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by AT&T -----Original Message----- From: Vanessa Perez Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 23 Apr 2013 13:12:26 +0000 From: Sandy Cope-yokoyama Subject: [Histonet] Forwarded post for a histonet member: Subject: CLIA 24-hour gross review. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <6F81318C8F5F984D972778B918A7D769310563@CMCPBEXMAIL12.Childrens.med> Content-Type: text/plain; charset="us-ascii" I am the sole histologist in a gastroenterology laboratory situated in a GI clinic. Complying with the 24-hour grossing review by the pathologist is a challenge because my slides travel by courier across town to the pathologist to read. I receive specimens throughout the day and perform grossing in the early AM then again at 11 AM. My pathologist signs copies of the requisition that I fax over that contains the gross and sends them back to me. On a daily basis this works just fine. Fridays and holidays are a pickle but we manage. My question is the intent of the 24-hour review. The inspector cannot tell me why this was put in place, what the pathologist is supposed to do with this information and/or how to tell if it is accurate because he does not receive the slides until the following day which would be outside of the 24-hour review rule. Can anyone tell the intent of the rule? How does this rule improve patient care? Even in a hospital laboratory, once a biopsy is grossed and placed in a cassette, how on earth does looking at the written gross of how big the specimen is or what color it is impact further actions that can take place? What remedial action does CLIA expect? Thanks for your input, Gastro Gal Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 9 Date: Tue, 23 Apr 2013 11:42:43 -0500 From: "McAnn, Sherrian" Subject: RE: [Histonet] Blades To: "Hale, Meredith" , Message-ID: <61E2B58CECEF384094A363989D47C09009BA28B7@VHAV17MSGA2.v17.med.va.gov> Content-Type: text/plain; charset="us-ascii" I have been in histology and cutting for about 26 years now. I have used many types of blades, high and low profile. My favorite and I think the best ones are Surgipath Teflon coated high or low profile blades. I believe the high profile blades are the best but I do realize that not everyone has that option. I think if you tried them you wouldn't be disappointed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Monday, April 15, 2013 12:39 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 23 Apr 2013 16:50:27 +0000 From: "Hale, Meredith" Subject: [Histonet] New Lab To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0E828EC51C7CC445A51E53F81B64E8C722D557@s-irv-exchmb.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 24 ***************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From benbhayes <@t> hotmail.com Wed Apr 24 11:31:21 2013 From: benbhayes <@t> hotmail.com (Ben Hayes) Date: Wed Apr 24 11:31:26 2013 Subject: [Histonet] PRN Dermatopathology Histotech Needed - South of Nashville, TN Message-ID: We are looking for a PRN histotech for a small dermatopathology laboratory in Spring Hill, TN, 25 miles south of Nashville, TN. Minimum of 2 years of embedding, cutting, and staining skin tissue required. Work hours completely flexible. Excellent compensation. Low volume lab. Please send cv to benbhayes@hotmail.com if interested. Ben Hayes MD PhD Skin & Allergy Center http://www.skinallergycenter.com From sbaldwin <@t> mhhcc.org Wed Apr 24 12:22:23 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Apr 24 12:22:35 2013 Subject: [Histonet] PROSTATE LEVELS Message-ID: Hi Histonetters We are just getting into prostate biopsies and our pathologists would like us to get 3 levels, we are going to do this but can I charge GM4 x3 with a modifier for those?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From trathborne <@t> somerset-healthcare.com Wed Apr 24 14:31:24 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Apr 24 14:31:53 2013 Subject: [Histonet] Position in NJ Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076C69CA1B@smcmail02.somerset-healthcare.com> Community hospital in central NJ has FT, histotech position available, day shift. Preferred qualifications include: -ASCP HT or HTL -training with NAACLS approved laboratory on-line training program or ASCP recognized program for histology -three or more years' experience in a histology laboratory -ability to create, manipulate and utilize spreadsheets and word-processing programs For a more complete description, or to apply on line, go to https://careers-somerset-healthcare.icims.com/jobs/1032/job or forward a resume directly to me at trathborne@somerset-healthcare.com . Toni Rathborne Pathology Supervisor Somerset Medical Center From b427297 <@t> aol.com Wed Apr 24 14:41:15 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Apr 24 14:41:19 2013 Subject: [Histonet] 5 gal specimen container search In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB757076C69CA1B@smcmail02.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB757076C69CA1B@smcmail02.somerset-healthcare.com> Message-ID: <8D00F3AD149D8FB-1314-B9E6@webmail-m173.sysops.aol.com> Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' From joewalker <@t> rrmc.org Wed Apr 24 15:22:49 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Wed Apr 24 15:22:55 2013 Subject: [Histonet] RE: FNA preparation In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C2A@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C2A@isexstore03> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC17C538FF@RRMBX03.rrmc.local> >From a CLIA standpoint the medical technologists is qualified to perform this function. The key to success is to ensure that the technologist has ample practice at making the FNA slides, which are different than a peripheral smear. Smearing aspirated material takes practice and it is also important to make notations on the consistency of the aspirated material and how it spreads since that can have a bearing on the FNA diagnosis. That's my two cents from someone who has trained both medtechs and cytotechs to perform the function, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org http://www.rrmc.org/ Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Friday, April 19, 2013 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA preparation Good Morning fellow Histonetters!! I am hoping someone can answer this question for me. Is a General Lab Tech (working as an assistant) permitted to prepare FNA slides at the bedside, as well as stain them using the Wright Stain before the slides being to the Pathologist for interpretation? My thinking on it is that I believe that they should be able to because after all they make CBC slides and stain those. We are having a debate in our department and I would like to know what the correct answer is before I would implement any training for the General tech. Thanks ever so much!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From christina.thurby <@t> bms.com Wed Apr 24 17:12:40 2013 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Wed Apr 24 17:12:47 2013 Subject: [Histonet] Question about Cleaved Caspase 3 Message-ID: Hi all, I will be looking up some reference papers tonight, but for any experts in the field out there - does cleaved caspase 3 only stain apoptotic cells or will it also stain some cells that are undergoing the autolysis process? Does anyone have experience using this antibody in the dorsal root ganglion for NHP? Thanks! Christina Thurby Bristol Myers Squibb christina.thurby@bms.com 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From badzrosari <@t> yahoo.com Wed Apr 24 18:22:38 2013 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Wed Apr 24 18:22:45 2013 Subject: [Histonet] vacuum sealers Message-ID: <1366845758.40871.YahooMailNeo@web121503.mail.ne1.yahoo.com> Hi histonetts.We would like to try using vacuum sealers for our tissue samples...but the one which can pack a sample mixed with a little formalin in it.Have someone tried this type of sealers??? From b427297 <@t> aol.com Wed Apr 24 20:41:48 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Apr 24 20:44:10 2013 Subject: [Histonet] vacuum sealers In-Reply-To: <1366845758.40871.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <1366845758.40871.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: <8D00F6D2F8B2A76-1428-1333A@webmail-d224.sysops.aol.com> We don't use a vacuum sealer, but we do heat seal Kapak bags with tissue in formalin, and store them for 15 years with no leaks. I've retrieved samples that were 14 years old, and they were as good as the day they went in the bag. I am not sure the vacuum would work without sucking out the formalin. Kapak bags can be ordered through various vendors in a multitude of sizes, and the heat sealers are not too expensive. Jackie O' -----Original Message----- From: Bernadette del Rosario To: histonet Sent: Wed, Apr 24, 2013 6:22 pm Subject: [Histonet] vacuum sealers Hi histonetts.We would like to try using vacuum sealers for our tissue samples...but the one which can pack a sample mixed with a little formalin in it.Have someone tried this type of sealers??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cornettl <@t> hotmail.com Thu Apr 25 09:39:16 2013 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Thu Apr 25 09:39:24 2013 Subject: [Histonet] Fite AFB Message-ID: We are working on a Fite's AFB, and are experiencing a strangeness. Was wondering if anyone else is experiencing issues at coverslipping. We are using an aqueous mounting media and when we apply it and the coverglass, the blue leaches from the tissue and spreads out under the coverglass (immediately). Any suggestions or comments would greatly be appreciated. Thanks, Lorraine Cornett HT(ASCP) HPC From cornettl <@t> hotmail.com Thu Apr 25 09:46:36 2013 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Thu Apr 25 09:46:42 2013 Subject: [Histonet] Fite AFB In-Reply-To: References: Message-ID: Please disregard....aqueous vs. synthetic mounting media! We figured it out about the time I hit the "send" button. Sorry for the bother. > From: cornettl@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 25 Apr 2013 10:39:16 -0400 > Subject: [Histonet] Fite AFB > > We are working on a Fite's AFB, and are experiencing a strangeness. Was wondering if anyone else is experiencing issues at coverslipping. We are using an aqueous mounting media and when we apply it and the coverglass, the blue leaches from the tissue and spreads out under the coverglass (immediately). > > Any suggestions or comments would greatly be appreciated. > > Thanks, > Lorraine Cornett HT(ASCP) > HPC _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbro22 <@t> lsuhsc.edu Thu Apr 25 14:25:00 2013 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Thu Apr 25 14:25:07 2013 Subject: [Histonet] Salary and recruiting for EM techs Message-ID: <2E3D5719BC42374E982EF0AD611F236286F37528@SH-ExchMB1.master.lsuhsc.edu> Greetings, We're evaluating salary for our EM technologists in preparation for hiring and are having a difficult time getting comparable information. Any input to the following questions would be greatly appreciated, and my response to them is included below: 1) What salary range does your facility offer for EM techs? 2) Is your facility private/public, hospital/reference lab/research lab? 3) Are they specialized histotechs or do they have their own job designation? 4) What are your hiring criteria? 5) Have you been able to recruit individuals with EM experience or train on-the-job? 6) Do the EM techs perform the full workload of processing, cutting, scoping, and photography? At our facility: 1) Currently employ 2 EM techs mid to high $40K (very top of our current salary range) 2) Pathology lab within state-run hospital 3) Employed as research/clinical associates (to distinguish from routine histotechs) 4) Lab science AS or general science BS required, otherwise similar to histotechs (license not required in LA) 5) Both from academic research backgrounds with advanced degrees--one with prior EM experience and MSA certification, one trained on-the-job 6) Perform processing, cutting, scoping, and photography with great degree of independence Thanks! Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology LSU Health Science Center - Shreveport Department of Pathology 1501 Kings Highway Shreveport, LA 71103 Tel. No. (318) 675-5872 jbro22@lsuhsc.edu From Diana.Harris <@t> viha.ca Thu Apr 25 15:32:17 2013 From: Diana.Harris <@t> viha.ca (Harris, Diana) Date: Thu Apr 25 15:32:22 2013 Subject: [Histonet] decalcifiers Message-ID: Does anyone use RegularCal-Immuno decalcifier from BBC? Can anyone tell me how it compares to Surgipath Decalcifier II for decalcifying time and IHC staining? Thanks Diana Harris QC & Method Development Technologist Dept. Of Laboratory Medicine Anatomical Pathology RJH -VIHA SI From rsrichmond <@t> gmail.com Thu Apr 25 18:33:30 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Apr 25 18:33:36 2013 Subject: [Histonet] Re: PROSTATE LEVELS Message-ID: S. Kathy Baldwin, SCT (ASCP) at Memorial Hospital and Health Care Center somewhere in southern Indiana asks: >>We are just getting into prostate biopsies and our pathologists would like us to get 3 levels, we are going to do this but can I charge GM4 x3 with a modifier for those??<< You need five levels, cut onto slides suitable for immunohistochemistry. Stain 1, 3, and 5 with H & E. Reserve 2 and 4 for possible immunostains, needed in about 10% of cases. I learned this while working in a big commercial prostate lab, and set it up in a lab processing a few prostate biopsies a week. In prostate biopsies, having to recut diminishes the usefulness of immunostains by about half. Another point: no more than two of the long (about 20 mm) cores per paraffin block. I can't comment on the billing issues Ms. Baldwin mentions. For CPT coding, you can code 88305 for each of as many bottles as you receive. The minimum is two (right and left) but it's nice if you can persuade your urologists to make additional designations (like apex, mid, base - on each side). Bob Richmond Samurai Pathologist Maryville TN From lpjones <@t> srhs-pa.org Fri Apr 26 08:41:45 2013 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Fri Apr 26 08:41:26 2013 Subject: [Histonet] Paraplast Xtra Melting Point? Message-ID: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> Happy Friday to all! For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem. A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point. We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag. Does anyone else have experiences to share? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts. Thank you in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From sdysart <@t> mirnarx.com Fri Apr 26 08:41:34 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Fri Apr 26 08:41:53 2013 Subject: [Histonet] Test Message-ID: Have there really been no emails in 2 days?? We just "upgraded" our email and I want to make sure I didn't lose this list... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From rjbuesa <@t> yahoo.com Fri Apr 26 08:52:07 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 26 08:52:14 2013 Subject: [Histonet] Paraplast Xtra Melting Point? In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> Message-ID: <1366984327.35669.YahooMailNeo@web163104.mail.bf1.yahoo.com> The "rule of thumb" is that the lower the paraffin melting point is?the higher the compression rate is. It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users. Ren? J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 9:41 AM Subject: [Histonet] Paraplast Xtra Melting Point? Happy Friday to all!? For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees?? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem.? A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point.? We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag.? Does anyone else have experiences to share?? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts.? Thank you in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMP <@t> stowers.org Fri Apr 26 10:29:40 2013 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Fri Apr 26 10:30:24 2013 Subject: [Histonet] Paraplast Xtra Melting Point? In-Reply-To: <1366984327.35669.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> <1366984327.35669.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <7A23A5A43C06284686B4868E88677530044EE0@MBSRV02.sgc.loc> On the back of the bag are the instructions : 52 degrees melting point: for best results, use at temperatures above 56 degrees. Would it make a difference if it melted at the lower temperature but, embedding is done at the warmer temperature? Just wondering. Thank you for your response. Nanne Marsh -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 26, 2013 8:52 AM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Xtra Melting Point? The "rule of thumb" is that the lower the paraffin melting point is?the higher the compression rate is. It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users. Ren? J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 9:41 AM Subject: [Histonet] Paraplast Xtra Melting Point? Happy Friday to all!? For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees?? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem.? A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point.? We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag.? Does anyone else have experiences to share?? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts.? Thank you in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janminshew <@t> yahoo.com Fri Apr 26 10:52:04 2013 From: janminshew <@t> yahoo.com (Janet Minshew) Date: Fri Apr 26 10:52:10 2013 Subject: [Histonet] The Golden Age Message-ID: <1366991524.83336.YahooMailWin8@web162102.mail.bf1.yahoo.com>
Hello everyone,

Well...I did it! I officially retired from my position at Leica Biosystems after over 20 years of service. It was very bittersweet, but it was becoming obvious that it was time to slow down a little. If you know me at all, you've probably guessed that I couldn't leave the field of Histotechnology cold-turkey. I've started a small consulting business, and I'll still be doing workshops and showing up at meetings. There is too much love in my heart to walk away completely.

If you need to reach me for any reason, please use the information below in my signature. It is also posted on the Membership directory page of the NSH website.

My best wishes go to each of you who are still carrying on this most important role and representing our field as professionals.

Kindest regards,

Jan Minshew
Specialized Histology Consulting, LLC
3404 Edwards Drive
Plano, TX 75025
214.587.3106
janminshew@yahoo.com
 From epeters2 <@t> gmu.edu Fri Apr 26 12:18:25 2013 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Fri Apr 26 12:18:40 2013 Subject: [Histonet] Paraplast Xtra Melting Point? In-Reply-To: <7A23A5A43C06284686B4868E88677530044EE0@MBSRV02.sgc.loc> References: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> <1366984327.35669.YahooMailNeo@web163104.mail.bf1.yahoo.com>, <7A23A5A43C06284686B4868E88677530044EE0@MBSRV02.sgc.loc> Message-ID: I have wondered about that myself recently. Noticed lower temperature needed in our waterbaths and melting at lower temperature in embedding center. Shiny drops form on top of our blocks, but not with the tissue. Esthr Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy Biology Program, Medical Technology Coordinator George Mason University ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Marsh, Nannette [NMP@stowers.org] Sent: Friday, April 26, 2013 11:29 AM To: 'Rene J Buesa'; Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraplast Xtra Melting Point? On the back of the bag are the instructions : 52 degrees melting point: for best results, use at temperatures above 56 degrees. Would it make a difference if it melted at the lower temperature but, embedding is done at the warmer temperature? Just wondering. Thank you for your response. Nanne Marsh -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 26, 2013 8:52 AM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Xtra Melting Point? The "rule of thumb" is that the lower the paraffin melting point is the higher the compression rate is. It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users. Ren? J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 9:41 AM Subject: [Histonet] Paraplast Xtra Melting Point? Happy Friday to all! For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem. A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point. We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag. Does anyone else have experiences to share? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts. Thank you in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Fri Apr 26 12:19:24 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Apr 26 12:20:21 2013 Subject: [Histonet] Per Diem Histotech and Part Time Grossing Tech Positions Available in Los Angeles, CA Message-ID: <12ECD7346266D74691EC2BFC75285E452F2DC60E@BFL323E10.pathmdlabs.local> Per Diem histotech position available in fast-paced private lab in West Hollywood. Varying night time hours (~11:00 pm-??) to cover for vacation, sickness, and heavy workload. There is a strong possibility that this position will turn into a part time or full time position in the future. The job consists of mainly embedding small biopsies (GI's, skins, prostate), but some cutting and manual special stains may also be needed. At least two years of experience in all aspects of histology is required. Part time grossing tech is also available, with the possibility of the position turning into full time in the future. The hours would be ~3 pm - 7:00 pm. Experience in grossing most types of tissue (no colon or large breast resections) is required. Please contact me at the direct line below and/or forward your resume to me if interested. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From TJohnson <@t> gnf.org Fri Apr 26 12:27:48 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Apr 26 12:27:53 2013 Subject: [Histonet] Re: The Golden Age Message-ID: <9F3CFEE76E51B64991C7485270890B404974FFCF@EX5.lj.gnf.org> Congratulations Jan! Thank you so much for you past contributions to our field and thank you again for continuing to contribute to the field in your retirement. If you and Gayle Callis (among others) are any indication, it's not looking too good for the rest of us who think someday we will retire and just veg. I'm hoping Jim Burchette leads the way. There are fish to catch! Best wishes to you in your new role, Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From rjbuesa <@t> yahoo.com Fri Apr 26 13:50:28 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 26 13:50:31 2013 Subject: [Histonet] Paraplast Xtra Melting Point? In-Reply-To: <7A23A5A43C06284686B4868E88677530044EE0@MBSRV02.sgc.loc> References: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> <1366984327.35669.YahooMailNeo@web163104.mail.bf1.yahoo.com> <7A23A5A43C06284686B4868E88677530044EE0@MBSRV02.sgc.loc> Message-ID: <1367002228.50993.YahooMailNeo@web163104.mail.bf1.yahoo.com> You can always melt the paraffin wax at any temperature above its melting point, but that does not change the characteristics of the wax and those with lower melting point are more compressible. Ren? J. From: "Marsh, Nannette" To: 'Rene J Buesa' ; "Jones, Laura" ; "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 11:29 AM Subject: RE: [Histonet] Paraplast Xtra Melting Point? On the back of the bag are the instructions : 52 degrees melting point:? for best results, use at temperatures above 56 degrees.? Would it make a difference if it melted at the lower temperature but, embedding is done at the warmer temperature?? Just wondering.? Thank you for your response. Nanne Marsh -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 26, 2013 8:52 AM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Xtra Melting Point? The "rule of thumb" is that the lower the paraffin melting point is?the higher the compression rate is. It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users. Ren? J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 9:41 AM Subject: [Histonet] Paraplast Xtra Melting Point? Happy Friday to all!? For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees?? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem.? A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point.? We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag.? Does anyone else have experiences to share?? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts.? Thank you in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com/for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Margraf <@t> cookchildrens.org Fri Apr 26 13:50:38 2013 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Fri Apr 26 13:50:44 2013 Subject: [Histonet] Job oppurtunity in Cobleskill NY Message-ID: <928719B9EBFA1C4686918B975FF84528D0028815@CCHCSMBX02.CCHCS.LDAP> Here is a message I am posting for a Histology educator who isn't a subscriber to Histonet. Please direct any questions about the position to COLONYP@cobleskill.edu Thanks and happy weekend. Linda M (Histonet administrator) Histotechnology Education Coordinator Institution: SUNY Cobleskill Location: Cobleskill, NY Posted: 03/15/2013 Application Due: Open Until Filled Type: Part-Time/Adjunct Salary: $20,000 to $21,000 USD Per Year Company Description: COLLEGE VALUES SUNY Cobleskill is seeking talented and innovative individuals to join the college community as we build a new vision for our second century as a college of agriculture and technology. Offering associate's and bachelor's degree programs, SUNY Cobleskill values innovative teaching and faculty committed to engaging pedagogies, collaborative and interdisciplinary endeavors, global perspectives, applied learning including internships and undergraduate research, and high levels of interaction with students. Job Description: This new position will work with the Histotechnician Program Director to maintain and enhance the academic and technical training of histotechnology students. Duties may include, but not be limited to, recruitment of students and new affiliate sites for internships; clinical coordination and site visits; classroom and laboratory teaching; collating and assessing data; and evaluating program effectiveness. Requirements: Bachelor's degree and three years of experience in Histotechnology required; master's degree preferred. The successful candidate will be a histotechnology professional who is certified by a national certifying agency. Excellent public relations, organization, decision making and creative skills are required. The candidate will have knowledge of educational methods and current accreditation and certification procedures. Additional Information: [*] Half-time, 11-month position to serve as the Education Coordinator for the Histotechnician A.A.S. program; begins August 2013. [*] This position offers full New York State benefits which are among the most comprehensive in the country. Salary Range: $20,000 - $21,000. [*] SUNY Cobleskill is committed to providing a safe and productive learning and living community for our students and employees. To achieve that goal, we conduct background investigations for all final candidates being considered for employment. Any offer of employment is contingent on the successful completion of the background check. Application Instructions: The application review process will begin immediately and will continue until a suitable candidate is found. Completed application consists of the following: 1. Letter of application, addressing qualifications, position responsibilities, and college values 2. Copies of official transcripts (originals required if hired) 3. Current CV 4. Names, emails and phone numbers of at least three professional references Application Information Contact: SUNY Cobleskill Fax: 518-255-5657 Online App. Form: http://cobleskill.interviewexchange.com/jobofferdetails.jsp?JOBID=38280 From NMP <@t> stowers.org Fri Apr 26 14:28:53 2013 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Fri Apr 26 14:29:43 2013 Subject: [Histonet] Paraplast Xtra Melting Point? In-Reply-To: <1367002228.50993.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4AE8039AEA096143B965CBC6D092166802351B3A28@EXCH2007.srhs-pa.org> <1366984327.35669.YahooMailNeo@web163104.mail.bf1.yahoo.com> <7A23A5A43C06284686B4868E88677530044EE0@MBSRV02.sgc.loc> <1367002228.50993.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <7A23A5A43C06284686B4868E88677530044F79@MBSRV02.sgc.loc> Thank you for the explanation Rene'. That makes perfect sense. Nanne ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, April 26, 2013 1:50 PM To: Marsh, Nannette; Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Xtra Melting Point? You can always melt the paraffin wax at any temperature above its melting point, but that does not change the characteristics of the wax and those with lower melting point are more compressible. Ren? J. From: "Marsh, Nannette" To: 'Rene J Buesa' ; "Jones, Laura" ; "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 26, 2013 11:29 AM Subject: RE: [Histonet] Paraplast Xtra Melting Point? On the back of the bag are the instructions : 52 degrees melting point: for best results, use at temperatures above 56 degrees. Would it make a difference if it melted at the lower temperature but, embedding is done at the warmer temperature? Just wondering. Thank you for your response. Nanne Marsh -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 26, 2013 8:52 AM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Xtra Melting Point? The "rule of thumb" is that the lower the paraffin melting point is the higher the compression rate is. It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users. Ren? J. From: "Jones, Laura" > To: "Histonet@lists.utsouthwestern.edu" > Sent: Friday, April 26, 2013 9:41 AM Subject: [Histonet] Paraplast Xtra Melting Point? Happy Friday to all! For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees? I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem. A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point. We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag. Does anyone else have experiences to share? I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts. Thank you in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com/for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Fri Apr 26 21:43:07 2013 From: cfitz <@t> 007group.com (Cathy) Date: Fri Apr 26 21:43:16 2013 Subject: [Histonet] AutoTech Embedder( Sakura) In-Reply-To: References: <1366233936.39505.YahooMailClassic@web184401.mail.bf1.yahoo.com> Message-ID: <006101ce42f0$f2ed8640$d8c892c0$@007group.com> I am also interested in information on the Sakura AutoTech Embedder. My manager has put on the capital equipment list along with the Sakura Express processor. Thanks, Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: Thursday, April 18, 2013 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: > From: Kiranjit Grewal > Date: April 17, 2013, 2:25:36 PM PDT > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AutoTech Embedder( Sakura) > > Hello Dear Colleagues, > > We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. > > Anyone currently using it and would like to share their experience is great. > > Thank you, > > -Kiran > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Apr 27 10:17:18 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 27 10:17:23 2013 Subject: [Histonet] AutoTech Embedder( Sakura) In-Reply-To: <006101ce42f0$f2ed8640$d8c892c0$@007group.com> References: <1366233936.39505.YahooMailClassic@web184401.mail.bf1.yahoo.com> <006101ce42f0$f2ed8640$d8c892c0$@007group.com> Message-ID: <1367075838.53003.YahooMailNeo@web163104.mail.bf1.yahoo.com> I have always used it and for me it is the best. Ren? J. From: Cathy To: 'Kiranjit Grewal' ; histonet@lists.utsouthwestern.edu Sent: Friday, April 26, 2013 10:43 PM Subject: RE: [Histonet] AutoTech Embedder( Sakura) I am also interested in information on the Sakura AutoTech Embedder.? My manager has put on the capital equipment list along with the Sakura Express processor. Thanks, Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: Thursday, April 18, 2013 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: > From: Kiranjit Grewal > Date: April 17, 2013, 2:25:36 PM PDT > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AutoTech Embedder( Sakura) > > Hello Dear Colleagues, >? > We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. >? > Anyone currently using it and would like to share their experience is great. >? > Thank you, >? > -Kiran > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Mon Apr 29 09:41:38 2013 From: cpyse <@t> x-celllab.com (Cindy Pyse) Date: Mon Apr 29 09:41:43 2013 Subject: [Histonet] FW: Yearly training Message-ID: <002c01ce44e7$a86887c0$f9399740$@x-celllab.com> Picked the wrong e-mail address on Friday, it was a long week. Hope everyone's Monday is going well. Thanks for any input. Cindy From: Cindy Pyse [mailto:cpyse@x-celllab.com] Sent: Friday, April 26, 2013 4:49 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what everyone's facilities train for annually. An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com From k.leigh.adams.865 <@t> gmail.com Mon Apr 29 10:39:03 2013 From: k.leigh.adams.865 <@t> gmail.com (k. leigh adams) Date: Mon Apr 29 10:39:08 2013 Subject: [Histonet] used Decloaker Message-ID: Good Morning, If anyone has a used Biocare Decloaker could you please contact me. K. Leigh (865)483-1800 thank you! From mbmphoto <@t> gmail.com Mon Apr 29 11:12:16 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Apr 29 11:12:26 2013 Subject: [Histonet] used Decloaker In-Reply-To: References: Message-ID: <8F107006-5435-4C68-A268-CAEE034DE18D@gmail.com> Hello Leigh, Yes! When I was working at UCSF, I used the Decloaker many times with various antigen retrieval agents. Not only for our paraffin sections, old archival paraffins sections and especially on 40um free-floating non-human primate and rat sections. It's user friendly and a reliable unit - we did not have the newest model. Hope this helps. Post-Script: I don't work for Biocare, just have respect for their products because in my hands the ones I used - worked. Maria Mejia San Francisco On Apr 29, 2013, at 8:39 AM, k. leigh adams wrote: > Good Morning, > > If anyone has a used Biocare Decloaker could you please contact me. > > K. Leigh (865)483-1800 > > thank you! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SohrabB1 <@t> ah.org Mon Apr 29 12:36:01 2013 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Mon Apr 29 12:36:08 2013 Subject: [Histonet] BIG PROBLEM Message-ID: <517E4D11.4347.0054.1@ah.org> Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles From lmdee1 <@t> yahoo.com Mon Apr 29 13:30:38 2013 From: lmdee1 <@t> yahoo.com (Linda) Date: Mon Apr 29 13:30:43 2013 Subject: [Histonet] BIG PROBLEM In-Reply-To: <517E4D11.4347.0054.1@ah.org> References: <517E4D11.4347.0054.1@ah.org> Message-ID: <1367260238.82176.YahooMailNeo@web164002.mail.gq1.yahoo.com> Hi, ? I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains. ? You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you. ? If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Linda Dee, HT(ASCP) ? ________________________________ From: Behnaz Sohrab To: histonet@lists.utsouthwestern.edu Sent: Monday, April 29, 2013 12:36 PM Subject: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Mon Apr 29 13:39:17 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Apr 29 13:39:21 2013 Subject: [Histonet] Yearly Training In-Reply-To: <20130429170432.7B47C1AA036@mail.pa-ucl.com> References: <20130429170432.7B47C1AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813C038E@PEITHA.wad.pa-ucl.com> Cindy- We also have a yearly competency packet. It follows JCAHO standards and includes direct observations, log documentation for QC and maintenance and problem solving through written exam. Systems covered are H&E, IHC, Special stains, processing/embedding, Cytology prep, and Misc. Histology. Nancy Schmitt Histology Coordinator Dubuque, IA ----------------------------------------------------- From: Cindy Pyse [mailto:cpyse@x-celllab.com] Sent: Friday, April 26, 2013 4:49 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what everyone's facilities train for annually. An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY. Cindy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From k.leigh.adams.865 <@t> gmail.com Tue Apr 30 08:29:33 2013 From: k.leigh.adams.865 <@t> gmail.com (k. leigh adams) Date: Tue Apr 30 08:29:37 2013 Subject: [Histonet] HIER pressure cookers.... Message-ID: Any input as to preferred instruments would be greatly appreciated... Leigh From brett_connolly <@t> merck.com Tue Apr 30 08:53:50 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Apr 30 08:53:55 2013 Subject: [Histonet] HIER pressure cookers.... In-Reply-To: References: Message-ID: Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lblazek <@t> digestivespecialists.com Tue Apr 30 08:58:58 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 30 09:01:42 2013 Subject: [Histonet] HIER pressure cookers.... In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange.digestivespecialists.com> I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER pressure cookers.... Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Apr 30 09:34:56 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Apr 30 09:35:00 2013 Subject: [Histonet] HIER pressure cookers.... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange.digestivespecialists.com> Message-ID: Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > I second that! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M > Sent: Tuesday, April 30, 2013 9:54 AM > To: k. leigh adams; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HIER pressure cookers.... > > Biocare Decloaker here !! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams > Sent: Tuesday, April 30, 2013 9:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HIER pressure cookers.... > > Any input as to preferred instruments would be greatly appreciated... > > Leigh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From Valerie.Hannen <@t> parrishmed.com Tue Apr 30 09:36:24 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Apr 30 09:36:29 2013 Subject: [Histonet] 5 gal specimen container search In-Reply-To: <8D00F3AD149D8FB-1314-B9E6@webmail-m173.sysops.aol.com> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From POWELL_SA <@t> mercer.edu Tue Apr 30 09:38:59 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Apr 30 09:39:01 2013 Subject: [Histonet] 5 gal specimen container search In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> References: <8D00F3AD149D8FB-1314-B9E6@webmail-m173.sysops.aol.com> <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> Message-ID: <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, April 30, 2013 10:36 AM To: 'Jackie O'Connor'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 5 gal specimen container search Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dingersoll <@t> aplaboratories.com Tue Apr 30 09:40:51 2013 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Tue Apr 30 09:40:57 2013 Subject: [Histonet] HIER pressure cookers.... Message-ID: <20130430074051.073ecbdb5144cf8a05e574ee22bfb11a.114d0c0283.wbe@email17.secureserver.net> dingersoll@aplaboratories.com -------- Original Message -------- Subject: RE: [Histonet] HIER From: "Blazek, Linda" <[1]lblazek@digestivespecialists.com> To: "Connolly, Brett M" , " adams" <[2]k. "[3]histonet@lists.utsouthwestern.edu" <[4]histonet@lists.utsouthwestern.edu< I second that! Linda Blazek HT (ASCP) GI Pathology of Dayton Digestive Speciali Phone: (937) 396-2623 Email: [5]lblazek@digestivespecialists.com -----Original Message----- From: [6]histonet-bounces@lists.utsouthwes mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh ad Subject: RE: [Histonet] HIER pressure cookers... Biocare Decloaker here !! Brett M. Connolly, Ph Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9]brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----O From: [10]histonet-bounces@lists.utsouthwestern.edu [[11]mailto:histonet-bounc k. leigh adams Sent: Tu To: [12]histonet@lists.utsouthwestern.edu Subject: [His Any input as to preferred ins appreciated... Leigh _________ Histonet mailing list [13]Histonet@lists.utsouthwe [14]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contai ns information of Merck & Co., Inc. (One Merck Drive, Whitehouse Statio contact information [15]http://www.merck.com/contact/contacts.html) that may confidential, proprietary copyrighted and/or legally privileged. It is i this messag received this message in e-mail and then delete it fro ________________________________________ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.uts ________________ Histonet mailing list [18]Histonet@lists.utsouthwestern [19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:lbla 2. 3D"mailto:k.leigh.adams.865@gmail.com" 3. 3D"mailto:histonet@lists.utsout 4. file://localhost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:bre 10. 3D"mailto:histonet-bounces@lists.u 11. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 12. 3D"mailto:histonet@lists.ut 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthwestern.edu/mailman/list 15. 3D"http://www.merck.com/conta 16. 3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18. ="mailto:Histonet@lists.utsouthwestern.edu" 19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ From gu.lang <@t> gmx.at Tue Apr 30 10:15:01 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 30 10:15:36 2013 Subject: AW: [Histonet] BIG PROBLEM In-Reply-To: <517E4D11.4347.0054.1@ah.org> References: <517E4D11.4347.0054.1@ah.org> Message-ID: <000301ce45b5$7d292a50$777b7ef0$@gmx.at> Too much blue is mostly a problem of too less red. Have you checked your Eosin? pH? Rinsing after eosin? My personal issue was sometimes too thin sections. Those looked also rather pale. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Behnaz Sohrab Gesendet: Montag, 29. April 2013 19:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles From kim-lake <@t> uiowa.edu Tue Apr 30 10:30:33 2013 From: kim-lake <@t> uiowa.edu (Lake, Kim S) Date: Tue Apr 30 10:31:05 2013 Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin Message-ID: I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 From Valerie.Hannen <@t> parrishmed.com Tue Apr 30 10:40:53 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Apr 30 10:40:58 2013 Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin In-Reply-To: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03> We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation. It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Tuesday, April 30, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From MMargiotta <@t> bmhmc.org Tue Apr 30 11:31:49 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Tue Apr 30 11:31:55 2013 Subject: [Histonet] cassette labelers Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> Hi All, We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Katy.Eichorn <@t> orovalleyhospital.com Tue Apr 30 12:10:42 2013 From: Katy.Eichorn <@t> orovalleyhospital.com (Eichorn, Katy) Date: Tue Apr 30 12:10:50 2013 Subject: [Histonet] HIER Pressure Cookers Message-ID: We use a Cuisinart Pressure Cooker that we purchased at Cell Marque. Basically they buy these pressure cookers and validate them at their facility, I think it was around $400. Works great! Katy Eichorn, HT (ASCP) | Histology Supervisor | Oro Valley Hospital | 1551 E. Tangerine Road | Oro Valley, AZ 85755 | Tel: 520 901-3914 | Cell: 520 891-5289 | Fax: 520 901-3905 | www.orovalleyhospital.com | katy.eichorn@orovalleyhospital.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intented only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Ashley.Troutman <@t> Vanderbilt.Edu Tue Apr 30 12:27:31 2013 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Apr 30 12:27:59 2013 Subject: [Histonet] Monitoring of IHC staining trends Message-ID: Hello Histonet, I have a question for the group at large. How are labs monitoring drift in IHC staining over time? Here's the scenario: You do lot to lot testing and everything looks fine until one day your pathologists are telling you that the CAM5.2 is too dark. Now, you've been looking at these slides every day for the last year and, sure enough, when you pull out a slide from last year's lot, it is significantly lighter. So what do we do about it? Do we revalidate the stain? Does anyone have a mechanism to monitor this better? What is the threshold for revalidation? Feedback from techs as well as any pathologists would be greatly appreciated. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu From TNMayer <@t> mdanderson.org Tue Apr 30 12:37:02 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Apr 30 12:37:08 2013 Subject: [Histonet] Older decal methods Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88015E7C6B@D1PWPEXMBX05.mdanderson.edu> Hi, I was wondering if anyone still uses the ion-exchange decal method and/or the electrolytic decal method? If so, could I get some pictures to put in my power points online? Also, to any of the new HT or HTL's are there any questions on the registry exam about those methods? My latest graduates didn't have but one about the ion-exchange method. If not then I can begin to phase them out. Thanks, Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org From khbarr <@t> mdanderson.org Tue Apr 30 12:48:38 2013 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Tue Apr 30 12:52:43 2013 Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 In-Reply-To: <694179cf-34a5-4474-b2f9-27a5436ceddd@DDPWPEXHUBCAS01.mdanderson.edu> References: <694179cf-34a5-4474-b2f9-27a5436ceddd@DDPWPEXHUBCAS01.mdanderson.edu> Message-ID: <65C8EC869E8581459DCD566079572FAC0B7ABECA@DCPWPEXMBX02.mdanderson.edu> What title do most use for someone who does gross and is not a PA? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 30, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PROBLEM (Linda) 3. Yearly Training (Nancy Schmitt) 4. HIER pressure cookers.... (k. leigh adams) 5. RE: HIER pressure cookers.... (Connolly, Brett M) 6. RE: HIER pressure cookers.... (Blazek, Linda) 7. Re: HIER pressure cookers.... (Jan Shivers) 8. RE: 5 gal specimen container search (Hannen, Valerie) 9. RE: 5 gal specimen container search (Shirley A. Powell) 10. RE: HIER pressure cookers.... (Dingersoll@aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lang) 12. Processor question - alcoholic formalin vs. buffered formalin (Lake, Kim S) 13. RE: Processor question - alcoholic formalin vs. buffered formalin (Hannen, Valerie) 14. cassette labelers (Margiotta-Watz, Michele) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Apr 2013 10:36:01 -0700 From: "Behnaz Sohrab" Subject: [Histonet] BIG PROBLEM To: Message-ID: <517E4D11.4347.0054.1@ah.org> Content-Type: text/plain; charset="us-ascii" Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 2 Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT) From: Linda Subject: Re: [Histonet] BIG PROBLEM To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <1367260238.82176.YahooMailNeo@web164002.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, ? I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains. ? You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you. ? If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Linda Dee, HT(ASCP) ? ________________________________ From: Behnaz Sohrab To: histonet@lists.utsouthwestern.edu Sent: Monday, April 29, 2013 12:36 PM Subject: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Apr 2013 18:39:17 +0000 From: Nancy Schmitt Subject: [Histonet] Yearly Training To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813C038E@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Cindy- We also have a yearly competency packet. It follows JCAHO standards and includes direct observations, log documentation for QC and maintenance and problem solving through written exam. Systems covered are H&E, IHC, Special stains, processing/embedding, Cytology prep, and Misc. Histology. Nancy Schmitt Histology Coordinator Dubuque, IA ----------------------------------------------------- From: Cindy Pyse [mailto:cpyse@x-celllab.com] Sent: Friday, April 26, 2013 4:49 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what everyone's facilities train for annually. An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY. Cindy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 30 Apr 2013 09:29:33 -0400 From: "k. leigh adams" Subject: [Histonet] HIER pressure cookers.... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Any input as to preferred instruments would be greatly appreciated... Leigh ------------------------------ Message: 5 Date: Tue, 30 Apr 2013 09:53:50 -0400 From: "Connolly, Brett M" Subject: RE: [Histonet] HIER pressure cookers.... To: "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Tue, 30 Apr 2013 09:58:58 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] HIER pressure cookers.... To: "Connolly, Brett M" , "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER pressure cookers.... Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 30 Apr 2013 09:34:56 -0500 From: Jan Shivers Subject: Re: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" Cc: "histonet@lists.utsouthwestern.edu" , "k. leigh adams" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > I second that! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M > Sent: Tuesday, April 30, 2013 9:54 AM > To: k. leigh adams; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HIER pressure cookers.... > > Biocare Decloaker here !! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams > Sent: Tuesday, April 30, 2013 9:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HIER pressure cookers.... > > Any input as to preferred instruments would be greatly appreciated... > > Leigh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ------------------------------ Message: 8 Date: Tue, 30 Apr 2013 10:36:24 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] 5 gal specimen container search To: 'Jackie O'Connor' , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> Content-Type: text/plain; charset="us-ascii" Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 9 Date: Tue, 30 Apr 2013 10:38:59 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] 5 gal specimen container search To: "Hannen, Valerie" , 'Jackie O'Connor' , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, April 30, 2013 10:36 AM To: 'Jackie O'Connor'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 5 gal specimen container search Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 30 Apr 2013 07:40:51 -0700 From: Subject: RE: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" , "Connolly, Brett M" , "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: <20130430074051.073ecbdb5144cf8a05e574ee22bfb11a.114d0c0283.wbe@email17.secureserver.net> Content-Type: text/plain; charset="utf-8" dingersoll@aplaboratories.com -------- Original Message -------- Subject: RE: [Histonet] HIER From: "Blazek, Linda" <[1]lblazek@digestivespecialists.com> To: "Connolly, Brett M" , " adams" <[2]k. "[3]histonet@lists.utsouthwestern.edu" <[4]histonet@lists.utsouthwestern.edu< I second that! Linda Blazek HT (ASCP) GI Pathology of Dayton Digestive Speciali Phone: (937) 396-2623 Email: [5]lblazek@digestivespecialists.com -----Original Message----- From: [6]histonet-bounces@lists.utsouthwes mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh ad Subject: RE: [Histonet] HIER pressure cookers... Biocare Decloaker here !! Brett M. Connolly, Ph Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9]brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----O From: [10]histonet-bounces@lists.utsouthwestern.edu [[11]mailto:histonet-bounc k. leigh adams Sent: Tu To: [12]histonet@lists.utsouthwestern.edu Subject: [His Any input as to preferred ins appreciated... Leigh _________ Histonet mailing list [13]Histonet@lists.utsouthwe [14]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contai ns information of Merck & Co., Inc. (One Merck Drive, Whitehouse Statio contact information [15]http://www.merck.com/contact/contacts.html) that may confidential, proprietary copyrighted and/or legally privileged. It is i this messag received this message in e-mail and then delete it fro ________________________________________ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.uts ________________ Histonet mailing list [18]Histonet@lists.utsouthwestern [19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:lbla 2. 3D"mailto:k.leigh.adams.865@gmail.com" 3. 3D"mailto:histonet@lists.utsout 4. file://localhost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:bre 10. 3D"mailto:histonet-bounces@lists.u 11. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 12. 3D"mailto:histonet@lists.ut 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthwestern.edu/mailman/list 15. 3D"http://www.merck.com/conta 16. 3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18. ="mailto:Histonet@lists.utsouthwestern.edu" 19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ ------------------------------ Message: 11 Date: Tue, 30 Apr 2013 17:15:01 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] BIG PROBLEM To: "'Behnaz Sohrab'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000301ce45b5$7d292a50$777b7ef0$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Too much blue is mostly a problem of too less red. Have you checked your Eosin? pH? Rinsing after eosin? My personal issue was sometimes too thin sections. Those looked also rather pale. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Behnaz Sohrab Gesendet: Montag, 29. April 2013 19:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 12 Date: Tue, 30 Apr 2013 15:30:33 +0000 From: "Lake, Kim S" Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 ------------------------------ Message: 13 Date: Tue, 30 Apr 2013 11:40:53 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "'Lake, Kim S'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03> Content-Type: text/plain; charset="us-ascii" We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation. It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Tuesday, April 30, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 14 Date: Tue, 30 Apr 2013 16:31:49 +0000 From: "Margiotta-Watz, Michele" Subject: [Histonet] cassette labelers To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 30 ***************************************** From LSebree <@t> uwhealth.org Tue Apr 30 13:30:44 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Apr 30 13:30:48 2013 Subject: [Histonet] RE: Monitoring of IHC staining trends In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF048027@UWHC-MBX12.uwhis.hosp.wisc.edu> Ashley, Every new lot of antibody we get in is run with some of our control tissues and compared to the current/previous lot staining. So we should be able to detect a change. Our policy is that minor changes to protocols are OK but anything major would require revalidation...we try not to go there. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Tuesday, April 30, 2013 12:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Monitoring of IHC staining trends Hello Histonet, I have a question for the group at large. How are labs monitoring drift in IHC staining over time? Here's the scenario: You do lot to lot testing and everything looks fine until one day your pathologists are telling you that the CAM5.2 is too dark. Now, you've been looking at these slides every day for the last year and, sure enough, when you pull out a slide from last year's lot, it is significantly lighter. So what do we do about it? Do we revalidate the stain? Does anyone have a mechanism to monitor this better? What is the threshold for revalidation? Feedback from techs as well as any pathologists would be greatly appreciated. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Tue Apr 30 13:42:30 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Apr 30 13:42:37 2013 Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 In-Reply-To: <65C8EC869E8581459DCD566079572FAC0B7ABECA@DCPWPEXMBX02.mdanderson.edu> References: <694179cf-34a5-4474-b2f9-27a5436ceddd@DDPWPEXHUBCAS01.mdanderson.edu> <65C8EC869E8581459DCD566079572FAC0B7ABECA@DCPWPEXMBX02.mdanderson.edu> Message-ID: Grossing Tech Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barr,Kaye H Sent: Tuesday, April 30, 2013 12:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 What title do most use for someone who does gross and is not a PA? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 30, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PROBLEM (Linda) 3. Yearly Training (Nancy Schmitt) 4. HIER pressure cookers.... (k. leigh adams) 5. RE: HIER pressure cookers.... (Connolly, Brett M) 6. RE: HIER pressure cookers.... (Blazek, Linda) 7. Re: HIER pressure cookers.... (Jan Shivers) 8. RE: 5 gal specimen container search (Hannen, Valerie) 9. RE: 5 gal specimen container search (Shirley A. Powell) 10. RE: HIER pressure cookers.... (Dingersoll@aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lang) 12. Processor question - alcoholic formalin vs. buffered formalin (Lake, Kim S) 13. RE: Processor question - alcoholic formalin vs. buffered formalin (Hannen, Valerie) 14. cassette labelers (Margiotta-Watz, Michele) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Apr 2013 10:36:01 -0700 From: "Behnaz Sohrab" Subject: [Histonet] BIG PROBLEM To: Message-ID: <517E4D11.4347.0054.1@ah.org> Content-Type: text/plain; charset="us-ascii" Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 2 Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT) From: Linda Subject: Re: [Histonet] BIG PROBLEM To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <1367260238.82176.YahooMailNeo@web164002.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, ? I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains. ? You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you. ? If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Linda Dee, HT(ASCP) ? ________________________________ From: Behnaz Sohrab To: histonet@lists.utsouthwestern.edu Sent: Monday, April 29, 2013 12:36 PM Subject: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Apr 2013 18:39:17 +0000 From: Nancy Schmitt Subject: [Histonet] Yearly Training To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813C038E@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Cindy- We also have a yearly competency packet. It follows JCAHO standards and includes direct observations, log documentation for QC and maintenance and problem solving through written exam. Systems covered are H&E, IHC, Special stains, processing/embedding, Cytology prep, and Misc. Histology. Nancy Schmitt Histology Coordinator Dubuque, IA ----------------------------------------------------- From: Cindy Pyse [mailto:cpyse@x-celllab.com] Sent: Friday, April 26, 2013 4:49 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what everyone's facilities train for annually. An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY. Cindy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 30 Apr 2013 09:29:33 -0400 From: "k. leigh adams" Subject: [Histonet] HIER pressure cookers.... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Any input as to preferred instruments would be greatly appreciated... Leigh ------------------------------ Message: 5 Date: Tue, 30 Apr 2013 09:53:50 -0400 From: "Connolly, Brett M" Subject: RE: [Histonet] HIER pressure cookers.... To: "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Tue, 30 Apr 2013 09:58:58 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] HIER pressure cookers.... To: "Connolly, Brett M" , "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER pressure cookers.... Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 30 Apr 2013 09:34:56 -0500 From: Jan Shivers Subject: Re: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" Cc: "histonet@lists.utsouthwestern.edu" , "k. leigh adams" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > I second that! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M > Sent: Tuesday, April 30, 2013 9:54 AM > To: k. leigh adams; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HIER pressure cookers.... > > Biocare Decloaker here !! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams > Sent: Tuesday, April 30, 2013 9:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HIER pressure cookers.... > > Any input as to preferred instruments would be greatly appreciated... > > Leigh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ------------------------------ Message: 8 Date: Tue, 30 Apr 2013 10:36:24 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] 5 gal specimen container search To: 'Jackie O'Connor' , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> Content-Type: text/plain; charset="us-ascii" Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 9 Date: Tue, 30 Apr 2013 10:38:59 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] 5 gal specimen container search To: "Hannen, Valerie" , 'Jackie O'Connor' , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, April 30, 2013 10:36 AM To: 'Jackie O'Connor'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 5 gal specimen container search Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 30 Apr 2013 07:40:51 -0700 From: Subject: RE: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" , "Connolly, Brett M" , "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: <20130430074051.073ecbdb5144cf8a05e574ee22bfb11a.114d0c0283.wbe@email17.secureserver.net> Content-Type: text/plain; charset="utf-8" dingersoll@aplaboratories.com -------- Original Message -------- Subject: RE: [Histonet] HIER From: "Blazek, Linda" <[1]lblazek@digestivespecialists.com> To: "Connolly, Brett M" , " adams" <[2]k. "[3]histonet@lists.utsouthwestern.edu" <[4]histonet@lists.utsouthwestern.edu< I second that! Linda Blazek HT (ASCP) GI Pathology of Dayton Digestive Speciali Phone: (937) 396-2623 Email: [5]lblazek@digestivespecialists.com -----Original Message----- From: [6]histonet-bounces@lists.utsouthwes mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh ad Subject: RE: [Histonet] HIER pressure cookers... Biocare Decloaker here !! Brett M. Connolly, Ph Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9]brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----O From: [10]histonet-bounces@lists.utsouthwestern.edu [[11]mailto:histonet-bounc k. leigh adams Sent: Tu To: [12]histonet@lists.utsouthwestern.edu Subject: [His Any input as to preferred ins appreciated... Leigh _________ Histonet mailing list [13]Histonet@lists.utsouthwe [14]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contai ns information of Merck & Co., Inc. (One Merck Drive, Whitehouse Statio contact information [15]http://www.merck.com/contact/contacts.html) that may confidential, proprietary copyrighted and/or legally privileged. It is i this messag received this message in e-mail and then delete it fro ________________________________________ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.uts ________________ Histonet mailing list [18]Histonet@lists.utsouthwestern [19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:lbla 2. 3D"mailto:k.leigh.adams.865@gmail.com" 3. 3D"mailto:histonet@lists.utsout 4. file://localhost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:bre 10. 3D"mailto:histonet-bounces@lists.u 11. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 12. 3D"mailto:histonet@lists.ut 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthwestern.edu/mailman/list 15. 3D"http://www.merck.com/conta 16. 3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18. ="mailto:Histonet@lists.utsouthwestern.edu" 19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ ------------------------------ Message: 11 Date: Tue, 30 Apr 2013 17:15:01 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] BIG PROBLEM To: "'Behnaz Sohrab'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000301ce45b5$7d292a50$777b7ef0$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Too much blue is mostly a problem of too less red. Have you checked your Eosin? pH? Rinsing after eosin? My personal issue was sometimes too thin sections. Those looked also rather pale. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Behnaz Sohrab Gesendet: Montag, 29. April 2013 19:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 12 Date: Tue, 30 Apr 2013 15:30:33 +0000 From: "Lake, Kim S" Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 ------------------------------ Message: 13 Date: Tue, 30 Apr 2013 11:40:53 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "'Lake, Kim S'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03> Content-Type: text/plain; charset="us-ascii" We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation. It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Tuesday, April 30, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 14 Date: Tue, 30 Apr 2013 16:31:49 +0000 From: "Margiotta-Watz, Michele" Subject: [Histonet] cassette labelers To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 30 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Tue Apr 30 13:42:48 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Apr 30 13:42:53 2013 Subject: [Histonet] cassette labelers In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> Message-ID: I suggest you look at either a heat transfer or laser etch product. The laser jet printers can be a bit of a maintenance issue. Also, you need to choose by the information needed on the cassette. 2D bar coding will take some time to print and requires precision in the printing. This is an important consideration in your selection of printer/labeler product. I have tested and used the Thermo PrintMate thermal transfer printer and General Data (GD) CL-01 laser cassette labeler. Both will produce a very usable and reliable 2D barcode. PrintMate can be used w/ multiple vendor cassettes. CL-01 must use a proprietary coated cassette. I choose the Thermo PrintMate, for a variety of reasons specific to process and lab, over the GD CL-01. The Microwriter is a different instrument from the PrintMate. I suggest the PrintMate over the Microwriter. I strongly suggest you set up your workflow, consider your specific needs and then ask each of the vendors to let you demo their units to see which one will work best to support your process. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: MMargiotta@bmhmc.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 30 Apr 2013 16:31:49 +0000 > Subject: [Histonet] cassette labelers > > Hi All, > We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. > > Thanks, > Michele > Histology Supervisor > BMHMC > 631-654-7192 > DISCLAIMER: > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. This communication may contain material protected by > the attorney-client privilege. If you are not the intended recipient > or the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dingersoll <@t> aplaboratories.com Tue Apr 30 13:47:18 2013 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Tue Apr 30 13:47:30 2013 Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 Message-ID: <20130430114718.073ecbdb5144cf8a05e574ee22bfb11a.bbbada2079.wbe@email17.secureserver.net> dingersoll@aplaboratories.com --- Subject: [Histonet] RE: Histonet Dige From: "Barr,Kaye H" <[1]khbarr@mdanderson.org> Date: Tue, April 30, To: "'[2]histonet@lists.utsouthwestern.edu'" <[3]histonet@lists.utsouthwestern.edu> What title do most use for someone who does gross and is not a P A? -----Original Message----- From: [[5]mailto:histonet-bounces@lists.utsouthwestern.edu] O [6]hi Sent: Tuesday, April 30, To: [7]histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol Send Histonet mailing list submissions to To subscribe or unsubscribe via the Worl [9]http://lists.utsouthwestern.edu/mailman/listinfo/hist or, via email, send a message with subject or body 'help' to [10]hist You can reach the pe [11]histonet-owner@lists.utsouthwestern.edu specific Today's To 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PR 3. Yearly Training (Nancy Schmitt) 4. HIER 5. RE: HIER pressure cookers 6. RE: HIER pressure cookers.... (Blazek, 7. Re: HIER pressure cookers.... (Jan Shivers) 8. 9. RE: 5 ga 10. RE: HIER pressu @aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lan 12. Processor question - alcoholic formalin vs. buffered forma lin (Lake, Kim S) 13. RE: Processor question - alcoholi formalin (Hannen, Valerie) 14. ----------- ----------------------------------------------------------- Me Date: Mon, 29 Apr 2013 10:36:01 -0700 From: "Behnaz So To: <[14]histonet@lists.utsouthwestern.edu> Mes Content-Type: text/plain; charset="us-ascii" Good Morning, We have two Leica H&E stainer( old xt-5010 an 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Ac Bluing : 45 sec. We done this for years!! last few mont Pantologists are complaining about GI Biopsies that they have TOO mu ch blue in cytoplasma and the Mucin is too purple and blue? Please any sugg Mean while we are trying the NEW kit from Leica for H&E think that is TOO pale?any of you has tried this new stain from Leci a? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 2 Dat From: Linda <[16]lmdee1@yahoo.com> Subject: Re: [Histon To: Behnaz Sohrab <[17]SohrabB1@ah.org>, "[18]histonet@lists.utsouthwestern.edu" Content-Type: text/plain; cha Hi, ? I would first check with y if they changed anything.? When som because the manufacturer have changed.? Thi stains. ? You may have to go back to alcohol, and eosin y.? Richard Allen 7 of their staining is excellent in these situat it back under control.? If you send them slides they out for you. ? If you are using Leica's selec lab out with because I was heavily sampl instead of the traditional purple.? I do 5 minut 30 dips in clarifier and 1 minute in blue, 55 sec in eos have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Lind ? ______________________________ From: Behnaz Sohrab <[20]Sohrab To: [21]histonet@lists.utsouthwestern.edu Sent: Monday, April 29, Subject: [Histonet] BIG PROBLEM G 5020). 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantol much blue in cy any suggestion?? think Lecia? Wit Behnaz Sohrab, Los Angeles _________ Histonet mailing list [22]Histonet@lists.utsouthwe [23]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 From: Nancy Schmitt Subject: [Histonet] Yearly Training To: "[24]histonet@lists.utsouthwestern.edu" Message-ID: <[26]906B4DA90E D1DB4DB6C7E94D7CEE6C36813C038E@PEITHA.wad.pa-ucl.com> Content- Cindy- We also ha standards and includes dir and maintenance and problem solv Systems covered are H&E, IHC, Special s processing/embedding, Cytology prep, and Misc. Histology. Nanc Histology Coordinator Dubuque, IA ------ From: Sent: Friday, April 26, 2013 4:49 PM To: '[28]histonet-bounces@lists.utso Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what eve annually. An example is HIPPA training. wanted to see what everyone else in hi advance for all responses. I hope everyone has Finally we are going to have a good weather weekend in We Cindy NOTI The information< recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the s that you have received it in error and then delete it along wit any attachments. Thank you. ----- Message: 4 Date: Tue, 30 Apr 2 From: "k. leigh adams" <[29]k.leigh.adams.865@gmail.com> Subject: To: [30]histonet@lists.utsouthwestern.edu Messag <[31]CAHot8dV4sR0aDAgb9+Ggbd6tH3CbrRbs0TdrB1BU0hob rHbYNQ@mail.gmail.com> Content-Type: text/plain; charset=ISO Any input as to preferred instruments would be greatly appreciated... Leigh -------------------- Message: 5 Date: Tue, 30 Apr 2013 09:53:50 -0 From: "Connolly, Brett M" <[32]brett_connolly@merck.com> Subject: RE: [Histonet] HIE To: "k. leigh adams" <[33]k.leigh.adams.865@gmail.com>, "[34]histonet@lists.utsouthwe <[35]histonet@lists.utsouthwestern.edu> Message-ID: <[36]C01C35B84DCDCE49BC60867E87F1C8FE68D4403906@USCTMXP51015.merck .com> Content-Type: text/plain; charset="us-ascii" Biocare Decloaker here !! Brett M. Connolly, Ph.D. P Merck & Co., Inc. PO Box West Point, PA 19486 [37]brett_connolly@merck.com T- 215-652-2501 F- 215 -----Original Mes From: [38]histonet-bounces@lists.utsouthwestern.edu [[39]mailto:histonet-bounces@lists.utso k. leigh adams Sent: Tuesday, April To: [40]histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER p Any input as to preferred instruments woul appreciated... Leigh ______________________ Histonet mailing list [41]Histonet@lists.utsouthwestern.edu [42]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notic contains info Station, information for affiliates is available at [43]http://www.merck.com/contact/contacts.html) th confidential, proprietary copyrighted and/or legally privil solely for the use of the individual or entity n you are not the intended recipient, and have error, please notify us immediately by repl from your system. ------------------------------ Message: 6 Da From: "Blazek, Linda" <[44]lblazek@digestivespecialists.c Subject: RE: [Histonet] HIER pressure cookers.... To leigh adams" <[46]k.leigh.adams.865@gmail.com>, "histonet@lists.utsouthwester <[47]histonet@lists.utsouthwestern.edu> Message-ID: < xchange.digestivespecialists.com> Content-Type: text I second that! Linda Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialist -----Original Message----- From: [[50]mailto:histonet-bounces@lists.utsouthwestern.edu] O Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; [51]histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER Biocare Decloaker here !! B Principal Scientist, Imaging Dept. Merc PO Box 4, WP-44K West Point, PA 19486 < href="mailto:brett_connolly@merck.com">brett_connolly@merck.com F- 215-993-6803 -----Original Message----- From: [52]histonet-bounces@lists.utsouthwester lto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh ad Sent: Tuesday, April 30, 2013 9:30 AM To: [54]histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input a appreciated... Lei _______________________________________________ Histonet mai [55]Histon [56]http://lists.utsouthwestern.edu/mailman/li Notice: This e-mail message, together with any a contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct c [57]http://www.merck.com/contact/contacts.h confidential, proprietary copyrighted and/or legally p is intended solely for the use of the individual or entity na this message. If you are not the intended recipient, and have receiv ed this message in error, please notify us immediately by reply e-mail and ______________________ Histonet mailing list [58]Histonet@lists.utsouthwestern.edu [59]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 From: Jan Shivers Subject: Re: [ To: "Blazek, Linda" <[60]lblazek@digestivespecialists.c Cc: "[61] <[62]histonet@lists.utsouthwestern.edu>, <[63]k.leigh.adams.865@gmail.com> Message-ID: wrote: > I second that! > > Linda Blazek HT (ASCP) > GI Pathology of Dayton > Dig > Phone: (937) 396-2623 > Email: > > > > -----Original Mes > From: [66]histonet-bounces@lists.utsouthwestern.edu [mailto: & Of Connolly, Brett M & > To: k. leigh adams; [68]histonet@lists.utsouthwe > Subject: RE: [Histonet] HIER pressure cookers....< > Biocare Decloaker here !! > > Brett > Principal Scientist, Imaging Dept. > > PO Box 4, WP-44K > West Point, > [69]brett_conno > T- 215-652-2501 > F- 215-993-6803 > > > > > > - > From: [70]histonet-bounces@lists.utsouthwestern.edu > [71]histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh > Sent: Tuesday, April 30, 2013 9:30 AM > To: histonet@lists.utsouthweste > Subject: [Histonet] HIER pressure cookers.... & > Any input as to preferred instruments would be greatly appre ciated... > > Leigh > __________________________ > Histonet mailing list > [72]Histonet@lists.utsouthwestern > [73]http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, c ontains > information of Merck & Co., Inc. (One Merck Drive, W Station, New > Jersey, USA 08889), and/or its affiliates information for > affiliates is available at confidential, > pr intended solely If y > not the intended recipient, and have received this messag error, please > notify us immediately by reply e-mail and the your system. > > > ____________ > Histonet mailing list > [76]http://lists.utsouthwestern.edu/mailman/listinfo > > _______________________________________ > Histonet mailing list > [77]Histonet@lists.utsouthwestern.edu > -- Jan Shivers Senior Scientist Pathology Teaching Program UMN Ve University of Minnesota College of Vet 1333 Gortner Ave. St. Paul, MN 55108 61 [79]shive003@umn.edu ------------------------------ Message: 8 Date: Tue, 30 Apr 2013 10:36:24 -0400 From: "Hannen, Valerie" &l Subject: RE: [Histonet] 5 gal specimen container search To: 'Jackie O'Connor' <[81]b427297 "[82]histonet@lists.utsouthwestern.edu" <[83]histonet@lists.utsouthwestern.edu&g Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexsto re03> Content-Type: text/plain; charset="us-ascii" We have ordered these from a company by the name of Medical. The lids are sold separately. Valerie -----Original Message----- From: [84]histonet-bounces@lists.utsouthwestern.ed histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: [86]histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search leakproof specimen? Ja _______________________________________________ [87]Histonet@lists.utsouthwestern.edu [88]http://lists.utsouthwestern.ed ========== "This email is intended solely for the us whom it is addressed and may contain informati privileged, confidential or otherwise exempt from disclosu under applicable law. If the reader of this email is not the delive hereby notified t copying of this communication have received this communication in e delete this message. Thank you" == ------------------------------ Message: 9 Date: Tue, From: "Shirley A. Powell" <[89]POWELL_SA@mercer.edu> Subject: RE To: "Hannen, Valerie" &l 'Jackie O'Connor' <[91]b427297@aol.com>, "[92]histonet@lists.utsouthwestern.edu" <[93]histonet@lists.utsouthwestern.edu Message-ID: <9BF995BC0E47744E9673A41486E24EE2579 B14884C@[94]MERCERMAIL.MercerU.loca Content-Type: text/plain; charset="us-ascii" purchased grea cheaper than the lab s Shirley -----Original Message-----< stonet-bounces@lists.utsouthwestern.edu [[96]mailto:histonet-bounces@lists.utsouthwestern Hannen, Valerie Sent: Tuesday, April 30, 2013 To: 'Jackie O'Connor'; [97]histonet@lists.utsouthwestern.edu Subject: RE: [H Jackie... Medical. T Valerie -----Origin From: [98]histonet-bounces@lists.utsouthwestern.edu [[99]mailto:histonet-bounces@list Jackie O'Connor Sent: Wednesda To: [100]histonet@lists.utsouthwestern.edu Subject: [Histone Anyone in histoland leakproof bucket, drum, pail for specimen? Jackie O' __ Histonet mailing list [101]Histonet@lists.ut [102]http://lists.utsouthwestern.edu/mailman/listinfo/hist ============= "This e whom it is address confidential or otherwis law. If the reader of this email the employee or agent responsible for deli intended recipient, you are hereby notified that distribution, or copying of this communication is strict prohibited. If you have received this communication in error, please imm ========= _______________________________________________ Histonet mailing list [103]Histonet@lists.utsouthwestern.edu [104]http://lists.utsouthweste ----------- Message: 10 Date: Tue, 30 Apr 2013 0 From: <[105]Dingersoll@aplaboratories.com> Subject: RE: [Histonet] HIE To: "Blazek, Linda" <[106]lblazek@digestivespecialists.com>, "Connolly, Brett M" <[107]brett_connolly@merck.com>, "k. leigh adams" <[108]k.leigh.adams.865@gmail.com> southwestern.edu" <[110]histonet@lists.utsouthwestern.edu> Message-ID: <[111]20130430074051.073ecbdb5144cf8a0 5e574ee22bfb11a.114d0c0283.wbe@email17.secureserver.net> Content-Type: text/plain; charset="utf-8" [112]dingersoll@aplaboratories.com -------- Original Message -------- From: "Blazek, Linda" <[1]lblazek@digestivespecialist To: "Connolly, Brett M" <[113]brett_connolly@merck.com>, " ada <[2]k. "[3][114]histonet@lists.utsouthwestern.edu" >&l I second that! Linda Blazek HT (ASCP) GI Digestive Speciali Phone: (937 Email: [5][116]lblazek@digestivespecialists.com -----Original Message----- Fro istonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sent: Tuesday, April 30, 2013 9:54 AM To Subject: RE: [Histonet] HIER pressure cookers... < Biocare Decloaker here !! Brett M. Connolly, Ph Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9][119]brett_connolly@merck.com T- 215-652- F- 215-993-6803 ---- From: [10][120]histonet-bounces@lists.utsouthwestern.edu Sent: Tu To: [12][121]histo Subject: [His Any input as to preferred ins appreciated... Leigh _________ Histonet [13]Histonet@[122] [14][123]http://lists.utsouthwestern.edu/mailman/listinf o/histonet Notice: This e-mail message, together with a contai ns information of Merck & Co., Inc. (One Whitehouse Statio contact information [15 confidential, proprietary cop is i this messag recei fro ________________________________________ Histo [16][125]Histonet@lists.utsouthwestern.edu [17]ht ________________ Histonet [18]Histonet@[126] [19][127]http://lists.utsouthwestern.edu/mailman/li stinfo/histonet References 1. 3D"mailto:lbla 2. 3D"[128]mailto:k.leigh.adams.865@gmail.com" 3. 3D"mailto:h ost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"[130]mailto:histonet-bounces@list 8. 3D"[131]mailto:histonet@lists.utsouthwestern.edu" 9 ="[132]mailto:histonet-bounces@ 12. 3D"mailto:histonet@lists.ut 13. ts.utsouthwestern.edu" 14. 3D"[134]lists.utsouthwestern.edu/mailman/list">http://[135]lists.utsou thwestern.edu/mailman/list 15. 16. 3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18. ="mailto 19. 3D"[137]lists.utsouthwestern.edu/mailman/listinfo">ht tp://[138]lists.utsouthwestern.edu/mailman/l ------------------------------ Messa Date: Tue, 30 Apr 2013 17:15:01 +0200 From: "Gudrun Lang Su To: "'Behnaz Sohrab'" <[140]SohrabB1@ah.org> Cc: [141]histonet@lists.utsouthwestern.edu Message-ID: <000301ce45b5$7d292a50$777b7ef0$@[142]gmx.at> Content-Type: text/plain; charset="iso-885 Too much blue is mostly a problem of too less red. My perso also rather p Gudrun -----Urspr?ngliche Nachricht----- [[144]mailto:histonet-bounces@lists.utsouthweste Auftrag von Behnaz Sohrab Gesendet: Montag, 29. An: [145]histonet@lists.utsouthwestern.edu Betreff: [Histonet] BIG PR Good Morning, We have two Leica H&E stainer( old xt- 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial AC seconds Bluing : 45 sec. We done this for years!! last f are complaining about GI Biopsies that the cytoplasma and the Mucin is too purple and bl Mean while we are trying the NEW kit from think that is TOO pale?any of you has tried With much application. Behnaz Sohra ------------------ Message: 12 Date: Tue, 30 Apr 2013 15:30:33 From: "Lake, Kim S" <[146] Subject: [Histonet] Processor question - a buffered formalin To: "[147]histonet@lists.utsouthwestern.edu" <[148]histo Message-ID: <[149]F3181E1428AD274EAEF67664C5B662AB25827126@itsnt443.iowa.uio wa.edu>< I attende (which was fantastic) an workshop. At this workshop I reali been having with sectioning could be due to Before microtomy we have to soak our b and I suspect that this is because we are over deh tissues in the during processing. Our first solution, which t cassettes sit in for about 8 hours until the overnight processing begins 900ml water, and that has been chuggi The reason we use alcoholic f buffered formalin has been lost to the mists wondering what you all use as a holding solution on you and why. Thanks! Kim Lake Labo Oral Pathology Laboratory University of Iowa Col Phone (319) 384 4433 Fax (319) 353 5569 ------------------------------ Message Date: Tue, 30 Apr 2013 11:40:53 -0400 From: "Hannen, Valer Subject: RE: [Histonet] Processor question - alco vs. buffered formalin To: "'Lake, Kim S'" & <[153]histonet@lists.utsouthwestern.edu> Message-ID: < ;450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03> Content-T We use 10 % Neutral B fixative and gives us really great fixa It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section C Parrish Medical Center 951 N. Washington Ave. Titusv Phone:(321) 268-6333 ext. 7506 Fax: (321) 2 [154]valerie.hann -----Original Message----- From: [155]h [[156]mailto:histonet-bounces@lists.utsouthwester Lake, Kim S Sent: Tuesday, April 30, 2013 11: To: [157]histon Subject: [Histonet] Processor questio buffered formalin I attended the Tr (which was fantastic) and sat in workshop. At this workshop I realized that having with sectioning could be due to our proc Before microtomy we have to soak our blocks fo and I suspect that this is because we are over dehydrating tissues in the during processing. Our first solution, which the casse ttes sit in for about 8 hours until the overnight processing begins, is alc water, and 3300ml has been chugging along The reason we use alcoholic formalin buffered formalin has been lost to the mists of time wondering what you all use as a holding solution on your proces and why. Thanks! Kim Lake Laboratory M Oral Pathology Laboratory University of Iowa College of Phone (319) 384 4433 Fax (319) 353 5569 Histonet mailing list [158]Histonet@lists. [159]http://lists.utsouthwestern.edu/mailman/listinfo/hi ================= "This email is intended solely for the use of the individual t whom it is addressed and may contain information that is priv under applic intended recipient o delivering the message to the hereby notified that any dissemination, copying of this communication is strictly prohibited have received this communication in error, please immediatel delete this message. Thank you" ========= ----------------- Message: 14 Date: Tue, 30 Apr 2013 16:31:4 From: "Margiotta-Watz, Michele" <[160]MMargiotta@bmhmc.org> Subject: [Histonet] casse To: "'[161]histonet@lists.utsouthwestern.edu'" <[162]histonet@lists.utsouthwestern.edu> Message-ID: <[163]230D0B9EC57D7A45A7A186C6AB4C7ABC 296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> Content-Type: text/plain; Hi All, We are looking to purchase some feedback on which ones you might re difference between Thermo's Printmate and Microwr the Printmate is just a newer version. Your input would be appreciated. Thanks, Michele Histology Su BMHMC 631-654-7192 DISCLAIMER: This are in they a by t recipient intended r and this in call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ ___________________ Histonet mailing list [164]Histonet@lists.utsouthwestern.edu< [165]http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 30 ********************** __________________________________________ Histonet mailing list [166]Histonet@lists.utsouthwestern.edu [167]http://lists.utsou References 1. 3D"mailto:khbar 2. 3D"mailto:histonet@lists.utsouthwestern.ed 3. 3D"mailto:hist 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists. 6. 3D"mailto:histonet-request@lists.utsouthwestern.edu" 7. 3D"mailto:histonet@lists.utsouthwestern.edu 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailm 10. 3D"mailto:histonet-request@lists.utsouthwestern.edu" 11. 3D"mailto:histonet-owner@lists.u 12. 3D"mailto:Dingersoll@aplaboratories.com" 13. 3D"mailto:SohrabB1@ah.org" 14. 3D"mailto:histonet@li 15. 3D"mailto:517E4D11.4347.0054.1@ah.org" 16. file://localhost/tmp/3D"m 17. 3D"mailto:SohrabB1@ah.o 18. 3D"mailto:histonet@lists.uts 19. 3D"mailto:1367260238.8 20. 3D"mailto:SohrabB1@ah.org" 21. 3D"mailto:histonet@lists.utsouthwestern 22. 3D"mailto:Histonet@lists.utsouthwestern.edu" 23. 3D"http://lists.utsouthwestern.edu/mailman/list 24. 3D"mailto:histon 25. 3D"mailto:histonet@lists.utsouthwestern.edu" 26. file://localhost/tmp/3D"mailto 27. 3D"mailto:cpyse@x-celllab.com" 28. file://localhost/tmp/3D"ma 29. 3D"mailto:k.lei 30. 3D"mailto:histonet@l 31. 3D"mailto:CAHot8dV4sR0aDAgb9+Ggbd6tH3CbrRbs0TdrB 32. 3D"mailto:brett_connolly@me 33. 3D"mailto:k.l 34. 3D"mailto:histonet@lists.utsouthwestern.edu" 35. 3D"mailto:histonet@lists.utsouthwestern 36. 3D"mailto:C01C35B84DCDCE49BC60867E87F1C8FE68D4403906@USCTMXP510 37. 3D"mailto:brett_connolly 38. 3D"mailto:histonet-bounces@lists.utsouthweste 39. 3D"mailto:hi 40. 3D"mailto:histonet@lists.utsouthwestern. 41. file://localhost/tmp/3D"mail 42. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 43. 3D"http://www.merck.co=/ 44. ="mailto:lblazek@digestivespecialists.com" 45. 3D"mailto:brett_connolly@merck.com" 46. file://localhost/tmp/3D"mailt 47. 3D"mailto:histonet@lists.utsouthwestern.edu 48. 3D"mailto:5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange. 49. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 50. 3D"mailto:histonet-bounces@lists. 51. 3D"mailto:histonet@lists.utsouthwestern.ed 52. 3D"mailto:hist 53. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 54. file://localhost/tmp/3D"mailto 55. 3D"mailto:Histonet@lists.utsouthwestern.edu" 56. 3D"http://lists.utsouthwester=/ 57. 3D"http://w=/ 58. file://localhost/tmp/3D"mail 59. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 60. ="mailto:lblazek@digestivespecialists.com" 61. 3D"mailto:histonet@lists.utsouthwestern.edu" 62. 3D"mailto:histo 63. 3D"mailto:k.leigh.adams.865@gmail.com 64. 3D"mailto:lblazek@diges 65. 3D"mailto:lblazek@digestivespecialists.com" 66. 3D"mailto:histonet-bounces@lists.utsouth 67. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 68. 3D"mailto:histonet@lists.utsouthwestern.edu" 69. 3D"mailto:brett_connolly@merck.com" 70. 3D"mailto:histonet-bounc 71. 3D"mailto:histonet-bounces@lists.utsouthwestern 72. ="mailto:Histonet@lists.utsouthwestern.edu" 73. 3D"http://lists.utsouthwestern.edu/mailman/list 74. 3D"http://www.merck.com/contact/contacts.html" 75. 3D"mailto:Histonet@lists.utsouthwestern.edu" 76. 3D"http://lists.utsouthwestern.edu/ 77. 3D"mailto:Hist 78. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 79. 3D"mailto:shive003@umn.edu" 80. 3D"mailto:Valerie.Hannen@parrishmed.com" 81. 3D"mailto:b427297@aol.com" 82. 3D"mailto:histonet@lists.utsouthwestern 83. file://localhost/tmp/3D"mailto 84. 3D"mailto:histonet 85. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 86. 3D"mailto: 87. 3D"mailto:Histonet@lists.utsouthwestern 88. 3D"http://lists.u=/ 89. file://localhost/tmp/3D" 90. 3D"mailto:Valerie.Hannen@parrishmed.com" 91. 3D"mailto:b427297@a 92. 3D"mailto:histonet@lists.utsouthw 93. file://localhost/tmp/3D" 94. 3D"http://MERCERMAIL.MercerU.local"/ 95. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 96. 3D"mailto:histonet-bou 97. 3D"mailto:histonet@lists.utso 98. 3D"mailto:histonet-bounces@lists.utsout 99. file://localhost/tmp/3D"mai 100. 3D"mailto:histonet@lists.utsout 101. 3D"mailto:Histonet@lists.utsouthwestern.edu" 102. 3D"http://lists.utsouthwestern.edu/mailm 103. 3D"mailto:Histonet@lists.utsouthwe 104. 3D"http://li=/ 105. 3D"mailto:Dingersoll@aplaboratories.c 106. 3D"mailto:lbl 107. 3D"mailto:brett_connolly@merck.com 108. ="mailto:k.leigh.adams.865@gmail.com" 109. 3D"mailto:histonet@lists.utsouthwestern.edu" 110. 3D"mailto:histonet@lists.utsouth 111. 3D"mailto:20130430074051.073ecbdb5144cf8a05e574ee22bfb11 112. 3D"mailto:dingersoll@aplaboratories.com" 113. file://localhost/tmp/3D 114. 3D"mailto:histonet@lists.utsout 115. 3D"mailto:histonet@lists.utsouthwestern.edu" 116. 3D"mailto:lblazek@digestivespe 117. 3D"http://lists.utsout"/ 118. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 119. 3D"mailto:brett_c 120. 3D"mailto:histonet-bounces@li 121. 3D"mailto:histonet@lists.utsouthwestern.edu" 122. 3D"http://lists.utsout"/ 123. 3D"http://lists.utsouthwestern.edu=/ 124. 3D"http://www.merck.com/contact/contacts.html" 125. 3D"mailto:Histonet@lists.utsou 126. 3D"http://lists.utsout"/ 127. 3D"http://lists.utsouthwester=/ 128. 3D"mailto:k.leigh.adams.865 129. 3D"http://lists.utsout"/ 130. file://localhost/tmp/3D"mai 131. 3D"mailto:histonet@lists.u 132. file://localhost/tmp/3D 133. 3D"mailto:Histonet@lists.utsouthwestern.edu" 134. file://localhost/tmp/3D"http 135. ="http://lists.utsout"/ 136. 3D"http://www.merck.com/conta" 137. 3D"http:// pa-ucl.com Tue Apr 30 14:03:29 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Apr 30 14:03:34 2013 Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 31 In-Reply-To: <20130430185234.6ABD21AA036@mail.pa-ucl.com> References: <20130430185234.6ABD21AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813C2679@PEITHA.wad.pa-ucl.com> Michele- I agree with Mr. DeSalvo. These vendors will be HAPPY to come in an set up a demo. Some products are better than others, but it needs to fit into your workflow to be successful. We ended up going with General Data and have been very happy with their product. Nancy Schmitt MLT, HT(ASCP) Histology Coordinator Dubuque, IA **************************************************************** Message: 7 Date: Tue, 30 Apr 2013 11:42:48 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] cassette labelers To: "Margiotta-Watz, Michele" , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I suggest you look at either a heat transfer or laser etch product. The laser jet printers can be a bit of a maintenance issue. Also, you need to choose by the information needed on the cassette. 2D bar coding will take some time to print and requires precision in the printing. This is an important consideration in your selection of printer/labeler product. I have tested and used the Thermo PrintMate thermal transfer printer and General Data (GD) CL-01 laser cassette labeler. Both will produce a very usable and reliable 2D barcode. PrintMate can be used w/ multiple vendor cassettes. CL-01 must use a proprietary coated cassette. I choose the Thermo PrintMate, for a variety of reasons specific to process and lab, over the GD CL-01. The Microwriter is a different instrument from the PrintMate. I suggest the PrintMate over the Microwriter. I strongly suggest you set up your workflow, consider your specific needs and then ask each of the vendors to let you demo their units to see which one will work best to support your process. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting **************************************************** > From: MMargiotta@bmhmc.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 30 Apr 2013 16:31:49 +0000 > Subject: [Histonet] cassette labelers > > Hi All, > We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. > > Thanks, > Michele > Histology Supervisor > BMHMC NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Tue Apr 30 15:14:41 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 30 15:14:45 2013 Subject: [Histonet] Monitoring of IHC staining trends In-Reply-To: References: Message-ID: <1367352881.22535.YahooMailNeo@web163104.mail.bf1.yahoo.com> In your example you acknowledge that your pathologist is right. In consequence you go back to your protocol and, more importantly, to the lot that cause your pathologist's concern and analyze if it contains the same amount of antibody. By the way, you should have tested the lot to avoid the problem in the first place. But maybe the problem is not in the lot but in the tissue or the processing protocol and you have to go over this problem also. I remember that when I switched from xylene to mineral oil, the IHC staining came stronger because I eliminated xylene that has an "extracting" effect on the?epitopes causing a weaker IHC reaction. When it was eliminated the dilution used for tissue processed with xylene resulted in a stronger IHC staining. My point is that you have to look to the whole process and try to find out "what is different" and probably causing the stronger reaction. Ren? J. From: "Troutman, Kenneth A" To: "Histonet@lists.utsouthwestern.edu" Sent: Tuesday, April 30, 2013 1:27 PM Subject: [Histonet] Monitoring of IHC staining trends Hello Histonet, I have a question for the group at large.? How are labs monitoring drift in IHC staining over time? Here's the scenario:? You do lot to lot testing and everything looks fine until one day your pathologists are telling you that the CAM5.2 is too dark.? Now, you've been looking at these slides every day for the last year and, sure enough, when you pull out a slide from last year's lot, it is significantly lighter.? So what do we do about it?? Do we revalidate the stain?? Does anyone have a mechanism to monitor this better?? What is the threshold for revalidation? Feedback from techs as well as any pathologists would be greatly appreciated. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN? 37232 ashley.troutman@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet