[Histonet] How to fix frozen section?

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Nov 6 14:58:13 CST 2012


I used acetone
René J.


________________________________
From: Amy Lee <amylee779 <@t> yahoo.com>
To: histonet <histonet <@t> lists.utsouthwestern.edu> 
Sent: Tuesday, November 6, 2012 2:41 PM
Subject: [Histonet] How to fix frozen section?

Hello,
 
I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me?
 
 
Thanks,
 
Amy
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