From pathlocums <@t> gmail.com Thu Nov 1 02:05:53 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Thu Nov 1 02:06:19 2012 Subject: [Histonet] POL labs In-Reply-To: <2334.208.62.167.196.1351712379.squirrel@webmail.realpages.com> References: <2334.208.62.167.196.1351712379.squirrel@webmail.realpages.com> Message-ID: <-6240746397703869563@unknownmsgid> POL's, which incidentally pay me well, are at least somewhat dangerous. Though I benefit greatly from them, I am also not going to sit on this forum and lie about how "deserving" they all are. There surely is over utilization going on. Denial of that fact does none of us any good. Sent from my iPhone On Oct 31, 2012, at 12:40 PM, Nicole Tatum wrote: > Let me start by sharing this: > > Definition of FREE ENTERPRISE > : freedom of private business to organize and operate for profit in a > competitive system without interference by government beyond regulation > necessary to protect public interest and keep the national economy in > balance. > > Key Word being For Profit. Health care is a commodity that is bought and > sold and the medical industry is big bucks for our economy. So what if a > POL is for profit, so are some hospitals, pharmaceutical companies, > pharmacies, and the local gas station. My point being is, just because a > POL is for profit does not mean that the facility does not offer the same > quality of care as a national laboratory who is also seeking profit. So, > as far as Im concerned the Doctor, owner, or medical director is able to > bill for any test he performs in his facility that is currently licensed > and regulated. I really dont think the setting should be a factor. We all > will see changes and cuts. I do not believe this thread has any thing to > do specifically with the election. Besides it doesnt really matter what > side of the fence your on. Cuts are comming, dare I say "rationing". Even > if socialized medicine does not get passed and Romney wins, Medicare will > have to decrease its allowable payouts each year. I personally am more > worried about what that will mean for our payscale. For those of you who > dont know me, I DO work in a POL lab. Im not bias, but I don't think the > location of my lab is relative to the fact that it shouldn't be allowed to > exist because its for profit. Just my thought. Happy Halloween to all. > > Nicole Tatum, HT ASCP > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Thu Nov 1 07:33:14 2012 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Nov 1 07:33:34 2012 Subject: [Histonet] Block/Slide placeholders Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760A72F5C1@VHAV09MSGA3.v09.med.va.gov> We use plain white index cards and cut with a paper cutter. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From AHutton <@t> dh.org Thu Nov 1 12:41:13 2012 From: AHutton <@t> dh.org (Hutton, Allison) Date: Thu Nov 1 12:43:57 2012 Subject: [Histonet] job opening in PA Message-ID: <38A56C4F4630D348A50B3720409270870E0FE67A@dhmail.dhorg.org> Doylestown Hospital is Doylestown, PA has the following position open: Job Title: Histology Tech - 1123 Req: 2012-2409 Location: Department: Laboratory Schedule: Mon-Fri varied start times 0530,0630,0730 Job Description: PURPOSE OF JOB: To assist the Histology and cytology Department in the preparation of tissue and cells for microscope pathology. Performs Histologic and Cytologic procedures as states in the Histology and Cytology Procedure Manuals. ESSENTIAL FUNCTIONS: 45% 1. Properly orients, embeds and sections tissues at 5 microns without artifacts or contaminants. 25% 2. Performs routine, special, frozen and immunohistochemical staining. Manually prepares all stock and working solutions needed to perform above stains (Stain list is available in Histology). 20% 3. Accurately and legibly accessions specimens with the use of the computer. Assists pathologist with frozen sections. Follows slides through the H&E stainer and appropriately distributes slides to the pathologists. 5% 4. Operates and maintains all histology equipment, recycles chemicals and disposes of pathological waste, troubleshoots outliers to help keep department running efficiently. 5% 5. Covers the Cytology Department in the absence of the cytotechnologist. Assists Cytology and Office with designated duties. For more information, please visit www.dh.org From Dingersoll <@t> aplaboratories.com Thu Nov 1 13:34:42 2012 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Thu Nov 1 13:34:48 2012 Subject: [Histonet] Disposal of used lab equipment Message-ID: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> We have upgraded some of our equipment in recent months and repla some VERY OLD equipment that is just taking up space now. I have contacted some of the used equipment man is interested in taking this equipment off our hands. My question is how do you dispose don't know if our local landfill will out there who will come haul it away? I appreciate any feedback. Donna S. Ingersoll, B.S., HTL, CT(ASCP) &n From contact <@t> excaliburpathology.com Thu Nov 1 13:43:10 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Nov 1 13:43:15 2012 Subject: [Histonet] Disposal of used lab equipment In-Reply-To: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> References: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> Message-ID: <1351795390.74666.YahooMailNeo@web5711.biz.mail.ne1.yahoo.com> I took some of mine to a place that buys scrap metal. They pay by the pound. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Dingersoll@aplaboratories.com" To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 1, 2012 1:34 PM Subject: [Histonet] Disposal of used lab equipment ? We have upgraded some of our equipment in recent months and repla= ced ? some VERY OLD equipment that is just taking up space now. ? = ? ? I have contacted some of the used equipment man= ufacturers and no one ? is interested in taking this equipment off our hands. ? ? ? My? question? is? how do you dispose = of used histology equipment.? I ? don't? know? if our local landfill will = take it.? Is there a service ? out there who will come haul it away? ? ? I appreciate any feedback. ? ? ? = ; ? ? Donna S. Ingersoll, B.S., HTL, CT(ASCP) ? ? &n= bsp; ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dingersoll <@t> aplaboratories.com Thu Nov 1 13:45:31 2012 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Thu Nov 1 13:45:39 2012 Subject: [Histonet] Disposal of used lab equipment Message-ID: <20121101114531.073ecbdb5144cf8a05e574ee22bfb11a.be3cdacf5b.wbe@email17.secureserver.net> We have the following equipment: Leica TP1050 (Ti Dako Autostainer (48 RMC Products MR3 Automated microtome Fisher Scientific IsoTemp oven 2 Ventanna Benchmark XT I just took over as lab manager a couple sure if all of the above are operational. I do kn Benchmark XTs do work and the Leica TP1050 does not work. Price is VERY negotiable. Donna S. Ing -------- Original Message -------- Subject: [Hi From: <[1]Dingersoll@aplaboratories.com> Date: Th To: [2]histonet@lists.utsouthwestern.edu We have upg ced some VERY = I have contacted some of the used equipment man= ufacture one is interested in taking this equipment off our hands. < My question is how do you dispose = of use don't know if our local landfill will = take service out there who will come haul it away? I appreciate any feedback. = ; Donna S. Ing &n= bsp; __________ Histonet mailing list [3]Histonet@lists.utsouthwestern [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:Dinge 2. 3D"mailto:histonet@lists.utsou 3. ="mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his From thoward <@t> unm.edu Thu Nov 1 13:53:57 2012 From: thoward <@t> unm.edu (Tamara A Howard) Date: Thu Nov 1 13:54:03 2012 Subject: [Histonet] Peptide for competition Message-ID: Several labs here use 21st Century Biochemicals for custom peptides: http://www.21stcenturybio.com/ Their tech support people are VERY helpful. Good luck! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From joewalker <@t> rrmc.org Thu Nov 1 14:09:26 2012 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Thu Nov 1 14:09:35 2012 Subject: [Histonet] Disposal of used lab equipment In-Reply-To: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> References: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1793284A@RRMBX03.rrmc.local> I don't know the type of equipment you are talking about but have you considered donating to a histotech school? Or even a high school that might be interested in taking their dissecting class to the next level? Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dingersoll@aplaboratories.com Sent: Thursday, November 01, 2012 2:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of used lab equipment We have upgraded some of our equipment in recent months and repla?d some VERY OLD equipment that is just taking up space now. =A I have contacted some of the used equipment man=acturers and no one is interested in taking this equipment off our hands. My question is how do you dispose = used histology equipment. I don't know if our local landfill will =ke it. Is there a service out there who will come haul it away? I appreciate any feedback. = Donna S. Ingersoll, B.S., HTL, CT(ASCP) &n=p; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From aakrasht <@t> yahoo.com Thu Nov 1 14:10:14 2012 From: aakrasht <@t> yahoo.com (Ali A Krasht) Date: Thu Nov 1 14:10:19 2012 Subject: [Histonet] Looking to Work Message-ID: <1351797014.86804.YahooMailNeo@web111209.mail.gq1.yahoo.com> Hi All I just got layoff by the company I was working for. I worked as a Histologist/ Supervisor for over 10 years.? If anyone is hiring in the Frisco or Dallas ?Texas area, please let me know. If you need more information or want to know my qualifications please check my LinkedIn Profile. www.linkedin.com/in/alikrasht www.linkedin.com/in/alikrasht =============================================== Omnipath Diagnostics Texas, Frisco, Texas 2008 - 2012 Histologist / Supervisor Ameripath (Dermpath Diagnostics Cockerell & Associates), Dallas, Texas 2004 - 2008 Histotechnologist / Histologist Analytical Food Laboratories, Grand Prairie, Texas 2003-2004 Microbiologist ================================================ Regards ? Ali A. Krasht http://NovellTrade.com http://NovoJeans.com 214 444 8319 ************************************************ The information transmitted is intended only for the person or entity to which it is addressed and may contain proprietary, business-confidential and/or privileged material. If you are not the intended recipient of this message you are hereby notified that any use, review, retransmission, dissemination, distribution, reproduction or any action taken in reliance upon this message is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Any views expressed in this message are those of the individual sender and may not necessarily reflect the views of the company. ************************************************ From billodonnell <@t> catholichealth.net Thu Nov 1 14:03:16 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Nov 1 14:12:05 2012 Subject: [Histonet] Disposal of used lab equipment In-Reply-To: <1351795390.74666.YahooMailNeo@web5711.biz.mail.ne1.yahoo.com> References: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> <1351795390.74666.YahooMailNeo@web5711.biz.mail.ne1.yahoo.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939583F6A3@chimsx08.CHI.catholichealth.net> Wow, so an AO 820 would buy a used car! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, November 01, 2012 1:43 PM To: Dingersoll@aplaboratories.com; Histonet Subject: Re: [Histonet] Disposal of used lab equipment I took some of mine to a place that buys scrap metal. They pay by the pound. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Dingersoll@aplaboratories.com" To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 1, 2012 1:34 PM Subject: [Histonet] Disposal of used lab equipment ? We have upgraded some of our equipment in recent months and repla= ced ? some VERY OLD equipment that is just taking up space now. ? = ? ? I have contacted some of the used equipment man= ufacturers and no one ? is interested in taking this equipment off our hands. ? ? ? My? question? is? how do you dispose = of used histology equipment.? I ? don't? know? if our local landfill will = take it.? Is there a service ? out there who will come haul it away? ? ? I appreciate any feedback. ? ? ? = ; ? ? Donna S. Ingersoll, B.S., HTL, CT(ASCP) ? ? &n= bsp; ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From rjbuesa <@t> yahoo.com Thu Nov 1 14:57:10 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 1 14:57:14 2012 Subject: [Histonet] Disposal of used lab equipment In-Reply-To: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> References: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> Message-ID: <1351799830.13965.YahooMailNeo@web163101.mail.bf1.yahoo.com> I think that if you contact the NSH they will have the addresses of some labs in the "Third World" that I am sure will be more than happy of accepting your "old" instruments that maybe they are "like new" for them. Ren? J. ________________________________ From: "Dingersoll@aplaboratories.com" To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 1, 2012 2:34 PM Subject: [Histonet] Disposal of used lab equipment ? We have upgraded some of our equipment in recent months and repla= ced ? some VERY OLD equipment that is just taking up space now. ? = ? ? I have contacted some of the used equipment man= ufacturers and no one ? is interested in taking this equipment off our hands. ? ? ? My? question? is? how do you dispose = of used histology equipment.? I ? don't? know? if our local landfill will = take it.? Is there a service ? out there who will come haul it away? ? ? I appreciate any feedback. ? ? ? = ; ? ? Donna S. Ingersoll, B.S., HTL, CT(ASCP) ? ? &n= bsp; ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Thu Nov 1 16:05:00 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Thu Nov 1 16:05:25 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 11/01/2012 and will not return until 11/07/2012. Note: For Cytology issues, please call Robin (day) at 8-421-5040, Wanda (day) 8-421-5426, or Eric (swing) 8-421-5405 For Histology issues, please call Mario (day) 8-421-4961, Barbara (swing) 8-421-4959, Bob (IHC) 8-421-4706, Kiran at 8-421-5404, or general histology client service at 8-421-5408 or Lotus Notes. From lyonm <@t> upstate.edu Thu Nov 1 16:10:28 2012 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Thu Nov 1 16:09:46 2012 Subject: [Histonet] Cryostat repair Message-ID: <003b01cdb875$51942480$f4bc6d80$@upstate.edu> We have a: Reichert HistoSTAT 975CJ cryostat microtome model: 855 serial #: 0819-3410 That needs attention. Any suggestions? Thanks Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice: 315-464-7253 Fax: 315-464-5572 From Barry.R.Rittman <@t> uth.tmc.edu Thu Nov 1 17:20:59 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Nov 1 17:21:02 2012 Subject: [Histonet] CLIA Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8237@UTHCMS1.uthouston.edu> Hi I wonder if anyone has information regarding material for CLIA such as printed material, videos etc. Thank you Barry From Norm.Burnham <@t> propath.com Thu Nov 1 17:25:29 2012 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Thu Nov 1 17:25:33 2012 Subject: [Histonet] CLIA Message-ID: <82C7248978CB50469FD6BA68EBBEFE6708DCC736@exchange.propathlab.com> http://www.cms.gov/Regulations-and-Guidance/Legislation/CLIA/index.html?redir ect=/clia/03_Interpretive_Guidelines_for_Laboratories.asp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, November 01, 2012 5:21 PM To: histonet Subject: [Histonet] CLIA Hi I wonder if anyone has information regarding material for CLIA such as printed material, videos etc. Thank you Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From sadey <@t> hotmail.ca Thu Nov 1 18:55:40 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Thu Nov 1 18:55:45 2012 Subject: [Histonet] Nails In-Reply-To: <12ECD7346266D74691EC2BFC75285E4501145DE3@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E4501145DE3@BFL323E10.pathmdlabs.local> Message-ID: We noticed for any tissues that have a tendency to float off: Leave over night at room temp to air dryThe next day heat in a separate oven at 56 degrees for 15minAllow the slides to cool back down to room temp.Then stain without using the oven on the stainer.Works great for fatty tissues Of course this is only great if you have a day to spare. :) > From: lcolbert@pathmdlabs.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 29 Oct 2012 18:09:46 +0000 > Subject: [Histonet] Nails > > Is there anyone out there who is an "expert" on nails - specifically how to get them to stay on the slides?? We use Nair to soften the nails, and we have used plain "plus" slides, chrome alum, Elmer's glue, and gelatin - all with very limited success. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tomgalati <@t> hsrl.org Thu Nov 1 21:37:42 2012 From: tomgalati <@t> hsrl.org (Tom Galati) Date: Thu Nov 1 21:35:43 2012 Subject: [Histonet] In need of a Microm microtome quick release clamp (aka block holder) Message-ID: Hello Netters, We are looking to purchase a quick release clamp for one of our Microm 310 microtomes. Does anyone have one for sale or have any thoughts where I can find one? The manufacturer has them, but they are ~ $1,000.00 new. Your help is appreciated. Regards, Tom Tom Galati Laboratory Director HSRL, Inc. 5930 Main Street Mount Jackson, VA 22842 540.477.4440 fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org ***Confidentiality Notice: This e-mail is strictly confidential and intended solely for the addressee. If you are not the intended recipient you must not use, disclose, or copy this transmission. If you have received this message in error, please contact the sender and delete the material from any computer, disc drive, diskette, or other storage device or media.*** From vanessaorsini <@t> msn.com Fri Nov 2 04:42:46 2012 From: vanessaorsini <@t> msn.com (Vanessa Orsini) Date: Fri Nov 2 04:42:51 2012 Subject: [Histonet] RNA isolation form stained slides Message-ID: Hello, I need to extract RNA for a RT-PCR after Laser Micro Dissection on xGal stained slides. I tried using sections from unfixed frozen organs. I fixed the sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37?C. After air drying I cut out with the LCM and extract RNA with the PicoPure kit from Applied Biosystem. So far I didn?t manage to get enough RNA. I tried to add RNase inhibitors to all the solutions but it didn?t help. Any idea/suggestion? Do someone think it would be better to do a LacZ antibody staining on FFPE sections and extract RNA with an appropriate kit? The RNase would they be less active? Thank in advance for any help you can give me J Vanessa From twebster <@t> CRH.org Fri Nov 2 06:50:06 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Fri Nov 2 06:50:13 2012 Subject: [Histonet] Devasting news on 88305TC component Message-ID: <7207186ED68FB542803CAF1CE6E82FF8032C89@exmb1.crh.org> It was cut 52%. I cannot believe this. http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From twebster <@t> CRH.org Fri Nov 2 06:53:30 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Fri Nov 2 06:53:36 2012 Subject: [Histonet] Devasting news on 88305TC component Message-ID: <7207186ED68FB542803CAF1CE6E82FF8032C93@exmb1.crh.org> Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From nicole <@t> dlcjax.com Fri Nov 2 07:05:01 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Fri Nov 2 07:05:12 2012 Subject: [Histonet] Softening toenails procedure Message-ID: <4780.208.62.167.196.1351857901.squirrel@webmail.realpages.com> Hi, I need a good procedure for softening toe nails to run on H&E. I have used Nair and KOH. Anyone have a good procedure that works well that they would like to share. Thanks, Nicole Tatum HT, ASCP From mayallf <@t> wave.co.nz Fri Nov 2 07:18:17 2012 From: mayallf <@t> wave.co.nz (Dr Mayall) Date: Fri Nov 2 07:18:23 2012 Subject: [Histonet] Free Diagnostic Pathology Software from the UK - Cancer reporting proformas added Message-ID: <53ae224047088eccd4903dd76ee2678b@wave.co.nz> Some of the list members might be interested in some recent enhancements to the free diagnostic pathology software that we have developed in the UK. We are adding proformas for complex cancer cases based on the The International Collaboration on Cancer Reporting (ICCR) proformas that are being developed. You can trial this free software (version 2.1) and download it from this website: www.freedp.org Many histopathology laboratories in the UK are using antiquated reporting software. Upgrading to a more modern system is expensive. Even the more modern systems often lack the technology needed for pathologists to efficiently report complex cases. They are often difficult to use across multiple sites. We have developed reporting software that overcomes some of these difficulties. Over the last year we have shared this open source software internationally via the website above and there are now hundreds of users around the World. Over the next year we are planning to incorporate more cancer proformas, and will release an updated version of the software in early 2013, via the website above. I would be very for any feed back regarding bugs or ideas for enhancements. Fred Mayall Consultant Histopathologist Taunton and Somerset NHS Trust From rjbuesa <@t> yahoo.com Fri Nov 2 08:50:19 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 2 08:50:26 2012 Subject: [Histonet] Softening toenails procedure In-Reply-To: <4780.208.62.167.196.1351857901.squirrel@webmail.realpages.com> References: <4780.208.62.167.196.1351857901.squirrel@webmail.realpages.com> Message-ID: <1351864219.3372.YahooMailNeo@web163105.mail.bf1.yahoo.com> Under separate cover I am sending something I wrote about this topic. Ren? J. ________________________________ From: Nicole Tatum To: histonet@lists.utsouthwestern.edu Sent: Friday, November 2, 2012 8:05 AM Subject: [Histonet] Softening toenails procedure Hi, I need a good procedure for softening toe nails to run on H&E. I have used Nair and KOH.? Anyone have a good procedure that works well that they would like to share. Thanks, Nicole Tatum HT, ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Fri Nov 2 10:09:18 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Fri Nov 2 10:09:29 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF8032C93@exmb1.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF8032C93@exmb1.crh.org> Message-ID: <1728673935529538408@unknownmsgid> That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > Devastating I meant > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Fri Nov 2 10:28:43 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Fri Nov 2 10:28:49 2012 Subject: [Histonet] Devasting news on 88305TC component Message-ID: http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.finlay@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -----Original message----- From: Davide Costanzo pathlocums@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: "Webster, Thomas S." twebster@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > Devastating I meant > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Nov 2 10:44:10 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Nov 2 10:44:17 2012 Subject: [Histonet] RNA isolation form stained slides In-Reply-To: References: Message-ID: Have you tried using more sections during extraction? Can you extract into a smaller volume? On Friday, November 2, 2012, Vanessa Orsini wrote: > > > > > > > Hello, > > I need to extract RNA for a RT-PCR after Laser Micro > Dissection on xGal stained slides. > > I tried using sections from unfixed frozen organs. I fixed the > sections in EtOH70% for 10min and then I stained them with xGal for 3h at > 37?C. > After air drying I cut out with the LCM and extract RNA with the PicoPure > kit > from Applied Biosystem. So far I didn?t manage to get enough RNA. > > I tried to add RNase inhibitors to all the solutions but it > didn?t help. > > > > Any idea/suggestion? > > Do someone think it would be better to do a LacZ antibody staining > on FFPE sections and extract RNA with an appropriate kit? The RNase would > they > be less active? > > > > Thank in advance for any help you can give me J Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From delsuec <@t> gmail.com Fri Nov 2 11:09:01 2012 From: delsuec <@t> gmail.com (Deloris Carter) Date: Fri Nov 2 11:09:04 2012 Subject: [Histonet] Control tissue Message-ID: Hi, Can anyone tell me if the control blocks we use for special stains and IHC need to have the accession number of the original case they came from on them? Does this info have to be put on the slides? Are there any CAP regulations about this? Thank you so much for your help. Deloris Carter HT(ASCP) Shawnee Mission Medical Center From jjohns3 <@t> cpallab.com Fri Nov 2 11:20:49 2012 From: jjohns3 <@t> cpallab.com (Johns, Jill) Date: Fri Nov 2 11:21:15 2012 Subject: [Histonet] ALK Positive lung slides Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C01530A05EC@EXCH02.wellspan.org> Does anybody have any lung tissue slides that are known to be positive for the ALK gene rearrangement or know of a source to obtain them? We are trying to validate this FISH assay are having a very difficult time finding any positive specimens (we have Abbott's positive control slides, but they do not look like "real" tissue specimens)....Thanks!! Jill A. Johns, MT(ASCP)SH, QCym, CCy Manager of Flow Cytometry and Molecular Diagnostics Central PA Alliance Laboratory (CPAL) 1803 Mt. Rose Ave., Suite C3/C4 York, PA 17403 phone: (717) 851-4320 fax: (717) 851-1450 email: jjohns3@cpallab.com ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From cls71877 <@t> gmail.com Fri Nov 2 11:32:06 2012 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Fri Nov 2 11:32:16 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: References: Message-ID: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Fri Nov 2 11:51:56 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Nov 2 11:52:04 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> Message-ID: AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From flnails <@t> texaschildrens.org Fri Nov 2 12:01:18 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Nov 2 12:01:23 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> Message-ID: Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From kkienitz <@t> orclinic.com Fri Nov 2 12:10:50 2012 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Fri Nov 2 12:15:30 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> , Message-ID: <41400FFE517878449D89114DD2526090087EA15B31@tocmail1.tocad.orclinic.com> Actually, the global payment for this code is being reduced by 33% according to the announcement. Regardless of the establishment, small lab; large volume lab; POL lab everyone will be taking a hit on this. Even if the POL labs all dried up and went away, the remaining labs may get the work but they will be doing it for a lot less reimbursement. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flnails@texaschildrens.org] Sent: Friday, November 02, 2012 10:01 AM To: 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Nov 2 12:26:29 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 2 12:26:33 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> Message-ID: <1351877189.5173.YahooMailNeo@web163105.mail.bf1.yahoo.com> EXACTLY SO! POLs started to offer "better?prices" to interested colleagues and by doing so work started to "drain" into POLs with the pointed out result = less work for Reference labs. Do you think that large ref. labs like Quest or Lab.Corp were going to "take that drainage" sitting on their hands? Sure not! They are business with billions at stake and money to lobby. This is the result. Blame the power of the?lobbyists! Policies and?even sometimes?democracy is tainted by big money. Tell that to the Supreme Court that has ruled that a large company is a?"person".? Ren? J. ________________________________ From: "Nails, Felton" To: 'Jesus Ellin' ; 'Cristi Rigazio' ; Brendal Finlay Cc: "histonet@lists.utsouthwestern.edu" ; "Webster, Thomas S." Sent: Friday, November 2, 2012 1:01 PM Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?!? Seriously!? 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class!? Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri Nov 2 12:50:18 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Nov 2 12:50:24 2012 Subject: [Histonet] Control tissue In-Reply-To: References: Message-ID: We put the accession number of the control tissue used on the slide and the information is also entered into our database. This eliminates the need for keeping an additional separate record for control tissue identification. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Friday, November 02, 2012 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control tissue Hi, Can anyone tell me if the control blocks we use for special stains and IHC need to have the accession number of the original case they came from on them? Does this info have to be put on the slides? Are there any CAP regulations about this? Thank you so much for your help. Deloris Carter HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Nov 2 13:11:11 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Nov 2 13:11:15 2012 Subject: [Histonet] ALK Positive lung slides In-Reply-To: <6D7752544B308D44A902C0BD0EC7BF5C01530A05EC@EXCH02.wellspan.org> References: <6D7752544B308D44A902C0BD0EC7BF5C01530A05EC@EXCH02.wellspan.org> Message-ID: Hi Jill, Have you tried using cases that you've already sent out to other labs and they've reported as ALK-positive? That is how we validated this assay in my lab. Most cases aren't "very" positive (you only have to find 15 cells out of a hundred that you count to call the case positive if a second observer agrees). You're supposed to look around for positive cells using your dual green/red filter. Since most tumor cells show a fused yellow or adjacent red & green signals, you really do have to look around for them a little. Because of that, I'd suggest doing the validation with cases that have a range of % of positve cells. If another lab calls a case positive at 25% positive cells then you'd want to get that result too most of the time. That being said, I can send you some de-identified slides from a case of metastatic (to a node) NSCLC that is "super" positive. I do think it is helpful to see what a case with a very high % of positive cells look like. This is what we're using as our positive control. We think it's better to have a tissue control than the cell cultured cells, processed into cell blocks, that are on the slides sold by Abbott. Send me your address and fedex # if interested. Mark On Fri, Nov 2, 2012 at 9:20 AM, Johns, Jill wrote: > Does anybody have any lung tissue slides that are known to be positive for > the ALK gene rearrangement or know of a source to obtain them? We are > trying to validate this FISH assay are having a very difficult time finding > any positive specimens (we have Abbott's positive control slides, but they > do not look like "real" tissue specimens)....Thanks!! > > Jill A. Johns, MT(ASCP)SH, QCym, CCy > Manager of Flow Cytometry and Molecular Diagnostics > Central PA Alliance Laboratory (CPAL) > 1803 Mt. Rose Ave., Suite C3/C4 > York, PA 17403 > phone: (717) 851-4320 > fax: (717) 851-1450 > email: jjohns3@cpallab.com > > > ______________________________________________________________________ > This e-mail has been scanned by Verizon Managed Email Content Service, > using Skeptic(tm) technology powered by MessageLabs. For more information > on Verizon's Managed Email Content Service, visit > http://www.verizonbusiness.com. > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Fri Nov 2 13:15:28 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 2 13:15:32 2012 Subject: [Histonet] Control tissue In-Reply-To: References: Message-ID: I would think that ANP.21100, 21366 might be applied by an inspector ( from older checklist so sorry numbers might have changed). But in any case I might suggest that some sort of numbering system to indicate the block sequence/identity and tissue type of the control is needed. Though you could probably write that accession number, it might use up too much writing space depending on what else you labeling requires. I think the checklist from last year had a specific stipulation about this. Probably on the CAP site - cap.org- by now. As I interpret it, you need a tracking of the source material and record keeping of that and what block was used as a control on what patient sample. You should also document that it worked :) Using a log to record the original source has usually worked out, and you can document it once as the original source with a tracking number to any prepared control blocks or control slides. I have always done testing of controls ( first and last of large batches) , or at least parallel, run noting the source, before use with a patient sample, and document the results are as expected- but I don't always see labs doing this. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 2 Nov 2012 11:09:01 -0500 > From: delsuec@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Control tissue > > Hi, > Can anyone tell me if the control blocks we use for special stains and IHC > need to have the accession number of the original case they came from on > them? Does this info have to be put on the slides? Are there any CAP > regulations about this? Thank you so much for your help. > Deloris Carter HT(ASCP) > Shawnee Mission Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Nov 2 13:25:39 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Nov 2 13:25:43 2012 Subject: [Histonet] RNA isolation form stained slides In-Reply-To: References: Message-ID: I would try getting more sections from LCM and extracting into 7.5 uL, if that volume will be enough to for your PCR reactions. On Friday, November 2, 2012, Vanessa Orsini wrote: > With the kit I'm extracting in 10ul....the problem is that I have too use > few stained cells isolated with the LCM...so even if I increase the number > of slides I'll never have a lot of material... > > Inviato da iPhone > > Il giorno 2 nov. 2012, alle ore 16:44, "Mark Tarango" < > marktarango@gmail.com 'marktarango@gmail.com');>> ha scritto: > > Have you tried using more sections during extraction? Can you extract > into a smaller volume? > > On Friday, November 2, 2012, Vanessa Orsini wrote: > >> >> >> >> >> >> >> Hello, >> >> I need to extract RNA for a RT-PCR after Laser Micro >> Dissection on xGal stained slides. >> >> I tried using sections from unfixed frozen organs. I fixed the >> sections in EtOH70% for 10min and then I stained them with xGal for 3h at >> 37?C. >> After air drying I cut out with the LCM and extract RNA with the PicoPure >> kit >> from Applied Biosystem. So far I didn?t manage to get enough RNA. >> >> I tried to add RNase inhibitors to all the solutions but it >> didn?t help. >> >> >> >> Any idea/suggestion? >> >> Do someone think it would be better to do a LacZ antibody staining >> on FFPE sections and extract RNA with an appropriate kit? The RNase would >> they >> be less active? >> >> >> >> Thank in advance for any help you can give me J Vanessa >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From jaylundgren <@t> gmail.com Fri Nov 2 13:26:40 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Nov 2 13:26:47 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <1351877189.5173.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> <1351877189.5173.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: The Gravy Train has left the station. Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Nov 2, 2012 at 12:26 PM, Rene J Buesa wrote: > EXACTLY SO! > POLs started to offer "better prices" to interested colleagues and by > doing so work started to "drain" into POLs with the pointed out result = > less work for Reference labs. > Do you think that large ref. labs like Quest or Lab.Corp were going to > "take that drainage" sitting on their hands? > Sure not! They are business with billions at stake and money to lobby. > This is the result. Blame the power of the lobbyists! > Policies and even sometimes democracy is tainted by big money. > Tell that to the Supreme Court that has ruled that a large company is > a "person". > Ren? J. > > > ________________________________ > From: "Nails, Felton" > To: 'Jesus Ellin' ; 'Cristi Rigazio' < > cls71877@gmail.com>; Brendal Finlay < > brendal.finlay@medicalcenterclinic.com> > Cc: "histonet@lists.utsouthwestern.edu" ; > "Webster, Thomas S." > Sent: Friday, November 2, 2012 1:01 PM > Subject: RE: [Histonet] Devasting news on 88305TC component > > Before you holler political and blame the candidates, ask yourself who was > hurt most by POL's? > Large reference labs. > With this change they will get back the business because it will not be > profitable to establish a POL. > Also they lobbied for and increase on the PC, reference have pathologist, > most POL's don't. > Just my thought > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin > Sent: Friday, November 02, 2012 11:52 AM > To: 'Cristi Rigazio'; Brendal Finlay > Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. > Subject: RE: [Histonet] Devasting news on 88305TC component > > AMEN!!!!!!!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio > Sent: Friday, November 02, 2012 9:32 AM > To: Brendal Finlay > Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. > Subject: Re: [Histonet] Devasting news on 88305TC component > > Political yet?! Seriously! 52%, while the PC is increased 2%... But in > case anyone wondered both candidates for President are looking for the > middle class! Unbelievable! > > Sent from my iPhone > > On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" < > brendal.finlay@medicalcenterclinic.com> wrote: > > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > > > > Brendal Finlay, HT (ASCP) > > Medical Center Clinic > > brendal.finlay@medicalcenterclinic.com > > 850.474.8758 > > http://medicalcenterclinic.com > > -----Original message----- > > From: Davide Costanzo pathlocums@gmail.com > > Date: Fri, 02 Nov 2012 10:09:18 -0500 > > To: "Webster, Thomas S." twebster@crh.org > > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > > > That is devastating! Do you have a link to this information? > > > > Sent from my iPhone > > > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > > > >> Devastating I meant > >> > >> > >> CONFIDENTIALITY NOTICE: > >> This e-mail message, including all attachments, is for the sole use > > of the > >> intended recipient(s) and may contain confidential and privileged > >> information. You may NOT use, disclose, copy or disseminate this > >> information. If you are not the intended recipient, please contact > > the > >> sender by reply e-mail immediately. Please destroy all copies of the > >> original message and all attachments. Your cooperation is greatly > >> appreciated. > >> Columbus Regional Hospital > >> 2400 East 17th Street > >> Columbus, Indiana > > 47201_______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt from > disclosure under applicable law. If you are not the intended recipient(s), > you are notified that the dissemination, distribution, or copying of this > message is strictly prohibited. If you receive this message in error, or > are not the named recipient(s), please notify the sender at either the > e-mail, fax, address, or telephone number listed above and delete this > e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SHUNTER <@t> beaumont.edu Fri Nov 2 17:41:07 2012 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Fri Nov 2 17:41:12 2012 Subject: [Histonet] RNA isolation form stained slides In-Reply-To: References: Message-ID: Have you tried to extract the RNA from the tissue without the stain- just to test your method? I am not familiar with the mechanics of the stain but you might be denaturing the RNA. Also are you sure there is enough message in the area you are trying to extract to get enough RNA? Maybe you need more material. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Orsini Sent: Friday, November 02, 2012 5:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RNA isolation form stained slides Hello, I need to extract RNA for a RT-PCR after Laser MicroDissection on xGal stained slides. I tried using sections from unfixed frozen organs. I fixed the sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37?C. After air drying I cut out with the LCM and extract RNA with the PicoPure kit from Applied Biosystem. So far I didn't manage to get enough RNA. I tried to add RNase inhibitors to all the solutions but it didn't help. Any idea/suggestion? Do someone think it would be better to do a LacZ antibody staining on FFPE sections and extract RNA with an appropriate kit? The RNase would they be less active? Thank in advance for any help you can give me J Vanessa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmhisto <@t> comcast.net Sat Nov 3 08:50:15 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Sat Nov 3 08:50:31 2012 Subject: [Histonet] Searching for OKC HT contact In-Reply-To: <512848527.1059881.1351950262293.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <2051998762.1059966.1351950615842.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Some time back, I'd been in touch with an HT at (I think) Baptist Hospital in Oklahoma City regarding training for a new histotech.? I met this lady at the Florida NSH meeting and we had been in touch for a time afterwards.? I have a candidate that needs to join a training program and I'd like to put him in touch with this person.?Please contact me at this address directly - and thank you! From gu.lang <@t> gmx.at Sat Nov 3 13:41:07 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Nov 3 13:41:15 2012 Subject: [Histonet] overfixation with formalin Message-ID: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang From jon2038433 <@t> maricopa.edu Sat Nov 3 13:42:10 2012 From: jon2038433 <@t> maricopa.edu (Jon Hannasch) Date: Sat Nov 3 13:42:18 2012 Subject: [Histonet] Urology special stains Message-ID: <66520FC6-9760-453D-8F36-A0D7D65F9522@maricopa.edu> Hello, I was curious if anyone had experience with urology tissue? What are some of the most popular special stains you performed? I'm trying to get more familiar with these kinds of tissues. Is there any histotech website that had helpful information for urology specimens? From ewj <@t> pigsqq.org Sat Nov 3 14:35:05 2012 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Sat Nov 3 14:35:16 2012 Subject: [Histonet] RNA isolation form stained slides In-Reply-To: References: Message-ID: <509571E9.2060000@pigsqq.org> I read one paper or abstract recently where sections were made at 50 microns then used successfully for RT-PCR. I am pretty sure that it was PRRS virus that they were pursuing. On 3:59, Mark Tarango wrote: > I would try getting more sections from LCM and extracting into 7.5 uL, if > that volume will be enough to for your PCR reactions. > > On Friday, November 2, 2012, Vanessa Orsini wrote: > > >> With the kit I'm extracting in 10ul....the problem is that I have too use >> few stained cells isolated with the LCM...so even if I increase the number >> of slides I'll never have a lot of material... >> >> Inviato da iPhone >> >> Il giorno 2 nov. 2012, alle ore 16:44, "Mark Tarango"< >> marktarango@gmail.com> 'marktarango@gmail.com');>> ha scritto: >> >> Have you tried using more sections during extraction? Can you extract >> into a smaller volume? >> >> On Friday, November 2, 2012, Vanessa Orsini wrote: >> >> >>> >>> >>> >>> >>> >>> Hello, >>> >>> I need to extract RNA for a RT-PCR after Laser Micro >>> Dissection on xGal stained slides. >>> >>> I tried using sections from unfixed frozen organs. I fixed the >>> sections in EtOH70% for 10min and then I stained them with xGal for 3h at >>> 37?C. >>> After air drying I cut out with the LCM and extract RNA with the PicoPure >>> kit >>> from Applied Biosystem. So far I didn?t manage to get enough RNA. >>> >>> I tried to add RNase inhibitors to all the solutions but it >>> didn?t help. >>> >>> >>> >>> Any idea/suggestion? >>> >>> Do someone think it would be better to do a LacZ antibody staining >>> on FFPE sections and extract RNA with an appropriate kit? The RNase would >>> they >>> be less active? >>> >>> >>> >>> Thank in advance for any help you can give me J Vanessa >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> > From Barry.R.Rittman <@t> uth.tmc.edu Sat Nov 3 14:32:14 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sat Nov 3 14:35:46 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu> Gudrun I would thank him for his opinion and move on. There has always been the unfortunate tendency to accept anything that is published or presented formally as if it were gospel. I am a lot more skeptical, give me proof. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Saturday, November 03, 2012 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Nov 4 02:02:52 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Nov 4 02:03:00 2012 Subject: AW: [Histonet] overfixation with formalin In-Reply-To: References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: <000901cdba62$cbaf5840$630e08c0$@gmx.at> This margin effect was especially seen in needle biopsies. In 2 mm cores. But he showed also a lymphnode slide, I would estimate 0,5-1 cm, with a margin-effect, that causes bad cutting in the center (loosened aereas). In this context he said, that a lymphnode sitting in higher concentrated formaldehyde over the weekend isn't properly fixed in the center because of the hardened surface. I'm not with his opinion. Gudrun Von: Charles.Scouten@LeicaBiosystems.com [mailto:Charles.Scouten@LeicaBiosystems.com] Gesendet: Samstag, 03. November 2012 23:20 An: gu.lang@gmx.at; histonet-bounces@lists.utsouthwestern.edu Betreff: Re: [Histonet] overfixation with formalin Is this due to the well known effects that formaldehyde penetrates tissues very slowly, at a rate of about 18mm/25 hours. And that autolysis (tissue breakdown) begins to occur immediately with extraction. If so, then in your tissue the center decayed before the fixative reached it. How large was the starting piece of tissue, how far was the center negative area from any direct exposure to formaldehyde? Was it precut in slices less then a mm thick, before being dropped in fixative? Cordially, Charles W. Scouten, Ph.D Product Specialist Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leicabiosystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: "Gudrun Lang" To: Date: 11/03/2012 01:42 PM Subject: [Histonet] overfixation with formalin Sent by: histonet-bounces@lists.utsouthwestern.edu _____ Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From gu.lang <@t> gmx.at Sun Nov 4 02:13:00 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Nov 4 02:13:05 2012 Subject: AW: [Histonet] overfixation with formalin In-Reply-To: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu> Message-ID: <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> Thanks for your support. Another theory about margin effect could be, that the methylenglycol in formalin is the main reagens. Isn't it possible, that it acts like other alcohols with dehydration at the surface (0,2-0,4 mm). But I think this effect is too weak to inhibit the center to be fixed properly. I still think, that it is too short fixation (no time for crosslinking) that causes "bad" centers. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rittman, Barry R Gesendet: Samstag, 03. November 2012 20:32 An: histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] overfixation with formalin Gudrun I would thank him for his opinion and move on. There has always been the unfortunate tendency to accept anything that is published or presented formally as if it were gospel. I am a lot more skeptical, give me proof. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Saturday, November 03, 2012 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Nov 4 10:09:00 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Nov 4 10:09:04 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: <1352045340.10835.YahooMailNeo@web163102.mail.bf1.yahoo.com> Gudrun: I think you should ask for a refund for the cost of the course and try to be contracted to teach the next course. You are right in all you said, and the "professor" is wrong. Ren? J. ________________________________ From: Gudrun Lang To: histonet@lists.utsouthwestern.edu Sent: Saturday, November 3, 2012 2:41 PM Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Nov 4 10:11:42 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Nov 4 10:11:47 2012 Subject: AW: [Histonet] overfixation with formalin In-Reply-To: <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu> <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> Message-ID: <1352045502.47112.YahooMailNeo@web163105.mail.bf1.yahoo.com> If methanol has reached a high concentration on the formalin solution it will act by itself both fixing (double fixation effect) and dehydrating. Ren? J. ________________________________ From: Gudrun Lang To: "'Rittman, Barry R'" Cc: Histonet@lists.utsouthwestern.edu Sent: Sunday, November 4, 2012 3:13 AM Subject: AW: [Histonet] overfixation with formalin Thanks for your support. Another theory about margin effect could be, that the methylenglycol in formalin is the main reagens. Isn't it possible, that it acts like other alcohols with dehydration at the surface (0,2-0,4 mm). But I think this effect is too weak to inhibit the center to be fixed properly. I still think, that it is too short fixation (no time for crosslinking) that causes "bad" centers. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rittman, Barry R Gesendet: Samstag, 03. November 2012 20:32 An: histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] overfixation with formalin Gudrun I? would thank him for his opinion and move on. There has always been the unfortunate tendency to accept anything that is published or presented formally as if it were gospel. I am a lot more skeptical, give me proof. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Saturday, November 03, 2012 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Nov 4 06:23:13 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Nov 4 11:09:57 2012 Subject: [Histonet] Disposal of used lab equipment In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC1793284A@RRMBX03.rrmc.local> References: <20121101113442.073ecbdb5144cf8a05e574ee22bfb11a.a26fd98ae6.wbe@email17.secureserver.net> <3C2378778400AD448ADA6FD6BDB7CCCC1793284A@RRMBX03.rrmc.local> Message-ID: <4B34F151125143F3A8726117171FBCF8@HP2010> Email has been down for several days, so if someone has already answered this, sorry. If anyone wants to donate used (in working order) equipment to a NAACLS-accredited histotech program, it would be appreciated. Also any supplies that you are no longer using (dry dye powdered because you have gone automated, cassettes because they don't fit your new labeler or you no longer use that color, etc.). You can ask the Schools to write you out a receipt, and you can say how much the equipment or supplies are worth. At our hospital, as a non-profit, we are to "donate" to the community - man hours, supplies, copying, etc. for things like career days, job fairs, donating used equipment to World Relief, etc. We have a form on line that we can fill out. Here's the search site for NAACLS programs. Under Program Type, highlight either HT or HTL. You can put in a specific state, or your can leave it as All. http://naacls.org/search/programs.asp Remember, it has to be in good condition and usable. Schools don't have money to begin with, and definitely do not have money to dispose of someone else's junk. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Joe W. Walker, Jr. Sent: Thursday, November 01, 2012 3:09 PM To: Dingersoll@aplaboratories.com ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of used lab equipment I don't know the type of equipment you are talking about but have you considered donating to a histotech school? Or even a high school that might be interested in taking their dissecting class to the next level? Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dingersoll@aplaboratories.com Sent: Thursday, November 01, 2012 2:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of used lab equipment We have upgraded some of our equipment in recent months and repla?d some VERY OLD equipment that is just taking up space now. =A I have contacted some of the used equipment man=acturers and no one is interested in taking this equipment off our hands. My question is how do you dispose = used histology equipment. I don't know if our local landfill will =ke it. Is there a service out there who will come haul it away? I appreciate any feedback. = Donna S. Ingersoll, B.S., HTL, CT(ASCP) &n=p; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From lpwenk <@t> sbcglobal.net Sun Nov 4 06:26:31 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Nov 4 11:10:00 2012 Subject: [Histonet] Looking to Work In-Reply-To: <1351797014.86804.YahooMailNeo@web111209.mail.gq1.yahoo.com> References: <1351797014.86804.YahooMailNeo@web111209.mail.gq1.yahoo.com> Message-ID: <81CE3885AE0744A58214F3B9A716A168@HP2010> NSH has a jobs board - jobs available, and a place for histotechs to post their information. Start there. http://www.jobtarget.com/home/home.cfm?site_id=8282 Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Ali A Krasht Sent: Thursday, November 01, 2012 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking to Work Hi All I just got layoff by the company I was working for. I worked as a Histologist/ Supervisor for over 10 years. If anyone is hiring in the Frisco or Dallas Texas area, please let me know. If you need more information or want to know my qualifications please check my LinkedIn Profile. www.linkedin.com/in/alikrasht www.linkedin.com/in/alikrasht =============================================== Omnipath Diagnostics Texas, Frisco, Texas 2008 - 2012 Histologist / Supervisor Ameripath (Dermpath Diagnostics Cockerell & Associates), Dallas, Texas 2004 - 2008 Histotechnologist / Histologist Analytical Food Laboratories, Grand Prairie, Texas 2003-2004 Microbiologist ================================================ Regards Ali A. Krasht http://NovellTrade.com http://NovoJeans.com 214 444 8319 ************************************************ The information transmitted is intended only for the person or entity to which it is addressed and may contain proprietary, business-confidential and/or privileged material. If you are not the intended recipient of this message you are hereby notified that any use, review, retransmission, dissemination, distribution, reproduction or any action taken in reliance upon this message is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Any views expressed in this message are those of the individual sender and may not necessarily reflect the views of the company. ************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sun Nov 4 12:52:12 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sun Nov 4 12:52:20 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: I have always been directed to the C. Fox article when discussing matters of the chemical mechanics and affects of aqueous formalin solution. Whenever I need to clarify a question, I tend to refer back to that resource. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gu.lang@gmx.at > To: histonet@lists.utsouthwestern.edu > Date: Sat, 3 Nov 2012 19:41:07 +0100 > Subject: [Histonet] overfixation with formalin > > Hi histonetters! > > I'm just attending a histo-course, where the teacher told us his opinion > about overfixation. > > For him overfixation takes place in any formaldehyde solution with a > concentration above 5%. This should cause the margin-artefact, that leads to > false-positive IHC at the margins of the tissue and to false-negative > results in the center. The higher concetrated fixative should harden and > shrink the surface, so it cant be penetrated any more by the fixative. > > > > I told him about the publication of Cecil Fox, who saw shrinkage only in > solutions with formaldehyde concentration above 30% (I think) and said, that > the methanol-part is responsible for that. > > I believe, that these margin-artefacts are due to drying at the time of > biopsy or an effect of the needle-shot itself. (But believing is no > evidence) > > > > In our lab we use 8% formaldehyde as standard fixative, buffered with > low-molar phosphatebuffer. There are no complains from the doctors about > margins. > > > > Please help me with the histonet-wisdom. What's your opinion? > > > > Bye > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sun Nov 4 13:01:33 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sun Nov 4 13:01:38 2012 Subject: AW: [Histonet] overfixation with formalin In-Reply-To: <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at>, <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu>, <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> Message-ID: You're fine in my opinion- according to Fox. If you read the first paragraph as introduction, and then skip over to the section starting with "pentration paradox" to paragraph(s) 2-3 of page 846, I think your thoughts are addressed. I can send the pdf to anyone if they want to look it over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gu.lang@gmx.at > To: Barry.R.Rittman@uth.tmc.edu > Date: Sun, 4 Nov 2012 09:13:00 +0100 > Subject: AW: [Histonet] overfixation with formalin > CC: Histonet@lists.utsouthwestern.edu > > Thanks for your support. > Another theory about margin effect could be, that the methylenglycol in > formalin is the main reagens. Isn't it possible, that it acts like other > alcohols with dehydration at the surface (0,2-0,4 mm). But I think this > effect is too weak to inhibit the center to be fixed properly. I still > think, that it is too short fixation (no time for crosslinking) that causes > "bad" centers. > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rittman, > Barry R > Gesendet: Samstag, 03. November 2012 20:32 > An: histonet@lists.utsouthwestern.edu > Betreff: RE: [Histonet] overfixation with formalin > > Gudrun > I would thank him for his opinion and move on. > There has always been the unfortunate tendency to accept anything that is > published or presented formally as if it were gospel. > I am a lot more skeptical, give me proof. > Barry > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang > [gu.lang@gmx.at] > Sent: Saturday, November 03, 2012 1:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] overfixation with formalin > > Hi histonetters! > > I'm just attending a histo-course, where the teacher told us his opinion > about overfixation. > > For him overfixation takes place in any formaldehyde solution with a > concentration above 5%. This should cause the margin-artefact, that leads to > false-positive IHC at the margins of the tissue and to false-negative > results in the center. The higher concetrated fixative should harden and > shrink the surface, so it cant be penetrated any more by the fixative. > > > > I told him about the publication of Cecil Fox, who saw shrinkage only in > solutions with formaldehyde concentration above 30% (I think) and said, that > the methanol-part is responsible for that. > > I believe, that these margin-artefacts are due to drying at the time of > biopsy or an effect of the needle-shot itself. (But believing is no > evidence) > > > > In our lab we use 8% formaldehyde as standard fixative, buffered with > low-molar phosphatebuffer. There are no complains from the doctors about > margins. > > > > Please help me with the histonet-wisdom. What's your opinion? > > > > Bye > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Sun Nov 4 17:21:25 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Nov 4 17:21:43 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D201C1F@xmdb04.nch.kids> Hi Gudrun, Over-fixation is likely to decrease IPX staining at the edges (since it would require harsher antigen retrieval). I agree with you. Many of the edge affects with IPX and several special stains (including H&E and Modified Orcein (for HBSAg etc) on Liver bx) are more probably dur to air-drying of the biopsies prior to fixation (similar seen on cautery specimens) or compression due to the needle as you suggested. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, 4 November 2012 5:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Sun Nov 4 17:29:46 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Nov 4 17:30:08 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <000901cdba62$cbaf5840$630e08c0$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <000901cdba62$cbaf5840$630e08c0$@gmx.at> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D201C3C@xmdb04.nch.kids> I would have explained this as poorly fixed tissue with the mushiness due to alcohol fixation from the dehydrating alcohols. Alcohol shrinks the tissue (as it removes the water from the cells), making it very difficult to infiltrate with xylene and the wax giving a mushy centre. This is also common if tissue samples are too thick (where they are squeezing out of the cassette holes). Formalin forms crosslinks with cell constituents (proteins etc) resulting in a "scaffold-like" rigidity of the cells. This allows easier movement of processing solutions in and out of the cells with little collapse of the cells. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, 4 November 2012 7:03 PM To: Charles.Scouten@LeicaBiosystems.com Cc: Histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] overfixation with formalin This margin effect was especially seen in needle biopsies. In 2 mm cores. But he showed also a lymphnode slide, I would estimate 0,5-1 cm, with a margin-effect, that causes bad cutting in the center (loosened aereas). In this context he said, that a lymphnode sitting in higher concentrated formaldehyde over the weekend isn't properly fixed in the center because of the hardened surface. I'm not with his opinion. Gudrun Von: Charles.Scouten@LeicaBiosystems.com [mailto:Charles.Scouten@LeicaBiosystems.com] Gesendet: Samstag, 03. November 2012 23:20 An: gu.lang@gmx.at; histonet-bounces@lists.utsouthwestern.edu Betreff: Re: [Histonet] overfixation with formalin Is this due to the well known effects that formaldehyde penetrates tissues very slowly, at a rate of about 18mm/25 hours. And that autolysis (tissue breakdown) begins to occur immediately with extraction. If so, then in your tissue the center decayed before the fixative reached it. How large was the starting piece of tissue, how far was the center negative area from any direct exposure to formaldehyde? Was it precut in slices less then a mm thick, before being dropped in fixative? Cordially, Charles W. Scouten, Ph.D Product Specialist Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leicabiosystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: "Gudrun Lang" To: Date: 11/03/2012 01:42 PM Subject: [Histonet] overfixation with formalin Sent by: histonet-bounces@lists.utsouthwestern.edu _____ Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From latecor <@t> montevideo.com.uy Sun Nov 4 19:23:36 2012 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Sun Nov 4 18:22:55 2012 Subject: AW: [Histonet] overfixation with formalin C.Fox pdf article In-Reply-To: References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu> <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> Message-ID: <201211042223360593.00193DF9@smtp.montevideo.com.uy> On 04/11/2012 at 07:01 p.m. joelle weaver wrote: I will be pleased if someone could send to me a pdf copy of C.Fox article. Thanks in advance, Carlos Defeo Histotechnologist Montevideo,Uruguay >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Nov 5 01:03:20 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Nov 5 01:03:33 2012 Subject: AW: [Histonet] overfixation with formalin _ methylene glycol Message-ID: <000301cdbb23$a440ebe0$ecc2c3a0$@gmx.at> Tony, thank you for your response. Do you know about any research on the effect of methylene glycol on native tissue? I made a Google search and wasn't really successful. It's a low concentrated di-ol, only present in aequous solution. Because small and hydrophilic it will penetrate tissue fast. It's action on cellmembrane-lipids?? In formalin about 99% is methylene glycol and therefore gives a w/w % about 59%_ instead of 36% formaldehyde. (Perhaps comparible with 50% ethanol?) Hmm, questions... Gudrun -----Urspr?ngliche Nachricht----- Von: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Gesendet: Montag, 05. November 2012 00:21 An: 'gu.lang@gmx.at'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] overfixation with formalin Hi Gudrun, Over-fixation is likely to decrease IPX staining at the edges (since it would require harsher antigen retrieval). I agree with you. Many of the edge affects with IPX and several special stains (including H&E and Modified Orcein (for HBSAg etc) on Liver bx) are more probably dur to air-drying of the biopsies prior to fixation (similar seen on cautery specimens) or compression due to the needle as you suggested. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, 4 November 2012 5:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** From one_angel_secret <@t> yahoo.com Mon Nov 5 10:03:45 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Nov 5 10:03:50 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <41400FFE517878449D89114DD2526090087EA15B31@tocmail1.tocad.orclinic.com> References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> , <41400FFE517878449D89114DD2526090087EA15B31@tocmail1.tocad.orclinic.com> Message-ID: <1352131425.8108.YahooMailNeo@web112313.mail.gq1.yahoo.com> Hi Histonetters. ? I was curious as to what measures or thoughts anyone had regarding the news from CAP about CMS cutting the 88305 TC by 52%. ? This is a devastating thought and from the news letter it is supposed to start 1/1/13 and I dont see anywhere in here where it says specifically POL's. This loks to effect all of us. ? Here?s the news letter. Your thoughts are appreciated. ? Thanks ? Kim D ? ? CMS Cuts 88305 TC by 52%, Molecular Codes Go On CLFS November 1?Advancing its commitment to contain health care delivery costs, CMS announced a series of physician pay cuts impacting pathologists in the final 2013 Physician Fee Schedule (PFS) released today. Most notably, the agency lowered the technical component (TC) of the surgical pathology code 88305 by 52%, although it raised the professional component (PC) by 2%, beginning Jan. 1. This change alters the global payment for this code, which will decrease by 33% as a result of this revaluation. The agency also announced that the newly developed molecular pathology CPT codes would be placed on the Medicare Clinical Laboratory Fee Schedule (CLFS). A new CMS HCPCS II G-code was created for situations requiring physician interpretation and reporting of these tests for Medicare beneficiaries. The revaluation of the 88305 code?as well as other codes in this surgical pathology family?is not surprising. As directed by the health care reform law, CMS has been focused on scrutinizing high volume codes from all specialties as potentially overvalued services. Indeed, the 88305 code is not only high volume, but its TC has not been reviewed since valued in 2000 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr ? ? ________________________________ From: "Kienitz, Kari" To: "Nails, Felton" ; 'Jesus Ellin' ; 'Cristi Rigazio' ; Brendal Finlay Cc: "histonet@lists.utsouthwestern.edu" ; "Webster, Thomas S." Sent: Friday, November 2, 2012 1:10 PM Subject: RE: [Histonet] Devasting news on 88305TC component Actually, the global payment for this code is being reduced by 33% according to the announcement. Regardless of the establishment, small lab; large volume lab; POL lab everyone will be taking a hit on this.? Even if the POL labs all dried up and went away, the remaining labs may get the work but they will be doing it for a lot less reimbursement. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR? 97220 503.935.8311 kkienitz@orclinic.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flnails@texaschildrens.org] Sent: Friday, November 02, 2012 10:01 AM To: 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?!? Seriously!? 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class!? Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 5 10:10:36 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 5 10:10:47 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <1352131425.8108.YahooMailNeo@web112313.mail.gq1.yahoo.com> References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> , <41400FFE517878449D89114DD2526090087EA15B31@tocmail1.tocad.orclinic.com> <1352131425.8108.YahooMailNeo@web112313.mail.gq1.yahoo.com> Message-ID: <1352131836.32341.YahooMailNeo@web163103.mail.bf1.yahoo.com> Yes, it is a general reeduction. Ren? J. ________________________________ From: Kim Donadio To: "Kienitz, Kari" ; "Nails, Felton" ; 'Jesus Ellin' ; 'Cristi Rigazio' ; Brendal Finlay Cc: "histonet@lists.utsouthwestern.edu" ; "Webster, Thomas S." Sent: Monday, November 5, 2012 11:03 AM Subject: Re: [Histonet] Devasting news on 88305TC component Hi Histonetters. ? I was curious as to what measures or thoughts anyone had regarding the news from CAP about CMS cutting the 88305 TC by 52%. ? This is a devastating thought and from the news letter it is supposed to start 1/1/13 and I dont see anywhere in here where it says specifically POL's. This loks to effect all of us. ? Here?s the news letter. Your thoughts are appreciated. ? Thanks ? Kim D ? ? CMS Cuts 88305 TC by 52%, Molecular Codes Go On CLFS November 1?Advancing its commitment to contain health care delivery costs, CMS announced a series of physician pay cuts impacting pathologists in the final 2013 Physician Fee Schedule (PFS) released today. Most notably, the agency lowered the technical component (TC) of the surgical pathology code 88305 by 52%, although it raised the professional component (PC) by 2%, beginning Jan. 1. This change alters the global payment for this code, which will decrease by 33% as a result of this revaluation. The agency also announced that the newly developed molecular pathology CPT codes would be placed on the Medicare Clinical Laboratory Fee Schedule (CLFS). A new CMS HCPCS II G-code was created for situations requiring physician interpretation and reporting of these tests for Medicare beneficiaries. The revaluation of the 88305 code?as well as other codes in this surgical pathology family?is not surprising. As directed by the health care reform law, CMS has been focused on scrutinizing high volume codes from all specialties as potentially overvalued services. Indeed, the 88305 code is not only high volume, but its TC has not been reviewed since valued in 2000 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr ? ? ________________________________ From: "Kienitz, Kari" To: "Nails, Felton" ; 'Jesus Ellin' ; 'Cristi Rigazio' ; Brendal Finlay Cc: "histonet@lists.utsouthwestern.edu" ; "Webster, Thomas S." Sent: Friday, November 2, 2012 1:10 PM Subject: RE: [Histonet] Devasting news on 88305TC component Actually, the global payment for this code is being reduced by 33% according to the announcement. Regardless of the establishment, small lab; large volume lab; POL lab everyone will be taking a hit on this.? Even if the POL labs all dried up and went away, the remaining labs may get the work but they will be doing it for a lot less reimbursement. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR? 97220 503.935.8311 kkienitz@orclinic.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flnails@texaschildrens.org] Sent: Friday, November 02, 2012 10:01 AM To: 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?!? Seriously!? 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class!? Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com/ > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twebster <@t> CRH.org Mon Nov 5 10:11:02 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Mon Nov 5 10:11:12 2012 Subject: [Histonet] Devasting news on 88305TC component Message-ID: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org> It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame "client billing" the most. The government is tired of being the "pull through" business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From b-frederick <@t> northwestern.edu Mon Nov 5 10:22:02 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Nov 5 10:22:08 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1F9B426E@evcspmbx2.ads.northwestern.edu> Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies......Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 10:11 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame "client billing" the most. The government is tired of being the "pull through" business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Nov 5 10:25:48 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Nov 5 10:26:53 2012 Subject: [Histonet] Histology in Little Rock Arkansas Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA328A55999B@Mail2Node1.ad.uams.edu> Good Morning, We are currently looking for a registered HT or HTL to work in a busy university laboratory. We are at the University of Arkansas for Medical Sciences in Little Rock. This is a full time position requiring someone with a clinical Histology background with processing, embedding, sectioning and staining routine surgical specimens and biopsies. We also have a night shift to cover our bone marrows (up to 35 per night) and many of the biopsies. This is a challenging position in a growing laboratory with some of the latest equipment. Recruiters need not call or write as we are not allowed to use your services due to state government rulings. Please contact me directly by e-mail or check the UAMS website under technical jobs #50038510 to apply through the system. Thank You, Pam Marcum Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mcauliff <@t> umdnj.edu Mon Nov 5 10:28:44 2012 From: mcauliff <@t> umdnj.edu (Geoff) Date: Mon Nov 5 10:28:51 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: <5097E93C.4070907@umdnj.edu> I agree with Rene, Barry, etc. Geoff On 11/3/2012 2:41 PM, Gudrun Lang wrote: > Hi histonetters! > > I'm just attending a histo-course, where the teacher told us his opinion > about overfixation. > > For him overfixation takes place in any formaldehyde solution with a > concentration above 5%. This should cause the margin-artefact, that leads to > false-positive IHC at the margins of the tissue and to false-negative > results in the center. The higher concetrated fixative should harden and > shrink the surface, so it cant be penetrated any more by the fixative. > > > > I told him about the publication of Cecil Fox, who saw shrinkage only in > solutions with formaldehyde concentration above 30% (I think) and said, that > the methanol-part is responsible for that. > > I believe, that these margin-artefacts are due to drying at the time of > biopsy or an effect of the needle-shot itself. (But believing is no > evidence) > > > > In our lab we use 8% formaldehyde as standard fixative, buffered with > low-molar phosphatebuffer. There are no complains from the doctors about > margins. > > > > Please help me with the histonet-wisdom. What's your opinion? > > > > Bye > > Gudrun Lang > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From one_angel_secret <@t> yahoo.com Mon Nov 5 10:32:41 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Nov 5 10:32:50 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1F9B426E@evcspmbx2.ads.northwestern.edu> References: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org> <62C639732D3F274DACED033EBDF6ADAF1F9B426E@evcspmbx2.ads.northwestern.edu> Message-ID: <1352133161.15340.YahooMailNeo@web112307.mail.gq1.yahoo.com> 88305 is the most common code anywhere, hospitals POL. ________________________________ From: Bernice Frederick To: "Webster, Thomas S." ; "'histonet@lists.utsouthwestern.edu'" Sent: Monday, November 5, 2012 11:22 AM Subject: RE: [Histonet] Devasting news on 88305TC component Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies......Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 10:11 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame "client billing" the most. The government is tired of being the "pull through" business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information.? If you are not the intended recipient, please contact the sender by reply e-mail immediately.? Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Mon Nov 5 11:33:38 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Mon Nov 5 11:42:26 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <1352133161.15340.YahooMailNeo@web112307.mail.gq1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org><62C639732D3F274DACED033EBDF6ADAF1F9B426E@evcspmbx2.ads.northwestern.edu> <1352133161.15340.YahooMailNeo@web112307.mail.gq1.yahoo.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939583F8E2@chimsx08.CHI.catholichealth.net> I am not in a POL, nor private lab. 88305 is our bread and butter. Being part of a large national organization, some of their labs will weather it out, however, I am in a town of 33,000 people and the only local laboratory.. We are already in competition with clinicians who choose to send them to a "cheaper" outsource. A number of scenarios come to mind: "Cheap" labs will no longer be any cheaper - we get the business back (most everyone in town as an alimentary canal and skin exposed to too much Sun) and I keep my job" :) (Most everyone will retain their alimentary canals and skin so histology remains sustainable) "Cheap" labs continue to bill less, increasing their volume by draining ours away and I lose my job. :( "Corporate" starts housing regional laboratories, shutting down the smaller ones (like us) and I lose my job or I uproot my family and move to the regional. :! "Corporate" could continue to subsidize the smaller labs, draining resources from other hospitals or services. However, 88305 is only one of many, many, many cuts across the health care industry. There may be no monies to redirect and a lot of people lose their jobs across the system in all disciplines. :( :( "We" offer something the cheap labs cannot and we weather the storm and hope for the best. As to some of the comments made over the last few days about politics and politicians: I know who I support, but this issue is not even a factor. Healthcare reform is happening and will happen. Maybe in a different form, maybe not - but it is coming and in many ways, it is here. Example: Two years ago, the insurance company that covered my company health plan just abandoned their health care coverage. They cannot make a profit on it. We got another insurance company, and the coverage was less impressive, but the good news is I got to pay more for it! Another increase in premiums the next year and more slated for the upcoming enrollment. Its just the way it's going to be. Private option, public option, business option, but there is really no option. What am I doing? I've kept a bathtub full of water since Y2K and learned to cure a ham, bake bread and make cheese, beer and wine. I also have two boxes of band-aids, two botttles of hydrogen peroxide and a clean toothbrush. Got a NetFlix subscription. Got a bible for guidence and a copy of Atlas Shrugged as a warning indicator. Kids are all married off and the wife doesn't eat much. Let the apocalypse come! Have a greaty day! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Monday, November 05, 2012 10:33 AM To: Bernice Frederick; Webster, Thomas S.; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Devasting news on 88305TC component 88305 is the most common code anywhere, hospitals POL. ________________________________ From: Bernice Frederick To: "Webster, Thomas S." ; "'histonet@lists.utsouthwestern.edu'" Sent: Monday, November 5, 2012 11:22 AM Subject: RE: [Histonet] Devasting news on 88305TC component Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies......Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 10:11 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame "client billing" the most. The government is tired of being the "pull through" business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information.? If you are not the intended recipient, please contact the sender by reply e-mail immediately.? Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From JEllin <@t> yumaregional.org Mon Nov 5 12:09:12 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Nov 5 12:09:19 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <1352133161.15340.YahooMailNeo@web112307.mail.gq1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org> <62C639732D3F274DACED033EBDF6ADAF1F9B426E@evcspmbx2.ads.northwestern.edu> <1352133161.15340.YahooMailNeo@web112307.mail.gq1.yahoo.com> Message-ID: This is for all of not matter if you are a POL, Hospital, and Reference. But read the remainder of the other cuts that are coming down. We just need to do things smarter and also look at our process to improve, I still think we have a outlook,, it is not as bright in the past though. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Monday, November 05, 2012 9:33 AM To: Bernice Frederick; Webster, Thomas S.; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Devasting news on 88305TC component 88305 is the most common code anywhere, hospitals POL. ________________________________ From: Bernice Frederick To: "Webster, Thomas S." ; "'histonet@lists.utsouthwestern.edu'" Sent: Monday, November 5, 2012 11:22 AM Subject: RE: [Histonet] Devasting news on 88305TC component Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies......Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 10:11 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame "client billing" the most. The government is tired of being the "pull through" business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information.? If you are not the intended recipient, please contact the sender by reply e-mail immediately.? Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From andreahooper <@t> rocketmail.com Mon Nov 5 12:31:37 2012 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Mon Nov 5 12:31:45 2012 Subject: [Histonet] Snap frozen to FFPE Message-ID: <1352140297.29176.YahooMailNeo@web141404.mail.bf1.yahoo.com> Hi All, Do you have a protocol for formalin fixation (and processing to FFPE) of a previously snap frozen piece of tissue? Or advice as to the best method of snap freezing for such a process (assume isopentane slurry)? We have done this for several?tissues?with our standard fixation and processing as we would for fresh wet tissue and have noticed an artifact. Advice?is welcome. ? Thank you, Andrea? From Mary.Fontaine <@t> vrh.org Mon Nov 5 13:49:37 2012 From: Mary.Fontaine <@t> vrh.org (Mary Fontaine) Date: Mon Nov 5 13:49:44 2012 Subject: [Histonet] slide and cassette labeler Message-ID: Our hospital will be looking into a cassette are a small lab with a volume of about 4000 surgicals appreciate any suggestions on the company and model that has work will for others. This may be a stand alone saystem that is not hook up t References 1. 3D"mailto:mary.fontaine@vrh.org" From latecor <@t> montevideo.com.uy Mon Nov 5 14:57:41 2012 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Mon Nov 5 13:56:57 2012 Subject: [Histonet] Histonet] thanks for your cooperation In-Reply-To: <1352137461.36521.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu> <000e01cdba64$3629fdf0$a27df9d0$@gmx.at> <201211042223360593.00193DF9@smtp.montevideo.com.uy> <1352137461.36521.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <201211051757410468.00092BB7@smtp.montevideo.com.uy> Rene J Buesa *********** REPLY SEPARATOR *********** On 05/11/2012 at 09:44 a.m. Rene J Buesa wrote: Estimado Carlos: Adjunto está el trabajo de Fox et al y, como parece que te interesa el tema, te he adjuntado también dos trabajos míos sobre fijación con formol. Saludos René J. From: C.D.G. To: Histonet@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Sent: Sunday, November 4, 2012 8:23 PM Subject: RE: AW: [Histonet] overfixation with formalin C.Fox pdf article On 04/11/2012 at 07:01 p.m. joelle weaver wrote: I will be pleased if someone could send to me a pdf copy of C.Fox article. Thanks in advance, Carlos Defeo Histotechnologist Montevideo,Uruguay >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Nov 5 14:34:22 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Nov 5 14:34:27 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: References: <7207186ED68FB542803CAF1CE6E82FF8032F7F@exmb1.crh.org> <62C639732D3F274DACED033EBDF6ADAF1F9B426E@evcspmbx2.ads.northwestern.edu> <1352133161.15340.YahooMailNeo@web112307.mail.gq1.yahoo.com> Message-ID: <1352147662.38158.YahooMailNeo@web112317.mail.gq1.yahoo.com> Yes Jesus, Thats what I was saying in short. This code isnt specific to a private lab, so those who think its only going to be private labs hurt by this they are wrong. ? and while I appreciate your positive spin on this. This is beyond devistating. I have worked in labs already where because of cost operational cost alone, then add staff, the budget was treading the red. I have spent the last few years squeezing blood from turnips. ? It seems once again the lab has gotten the shaft. ? It is very disapointing that CAP during its battle managed to get the Pathologist PC ?a 2% increase, but such a huge HUGE decrease for?our TC. ? Someone please tell me a joke or something. Perferably one where a woman walks in a bar......................... ? ? ? ? ________________________________ From: Jesus Ellin To: 'Kim Donadio' ; Bernice Frederick ; "Webster, Thomas S." ; "'histonet@lists.utsouthwestern.edu'" Sent: Monday, November 5, 2012 1:09 PM Subject: RE: [Histonet] Devasting news on 88305TC component This is for all of not matter if you are a POL, Hospital, and Reference. But read the remainder of the other cuts that are coming down.? We just need to do things smarter and also look at our process to improve,? I still think we have a outlook,, it is not as bright in the past though. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Monday, November 05, 2012 9:33 AM To: Bernice Frederick; Webster, Thomas S.; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Devasting news on 88305TC component 88305 is the most common code anywhere, hospitals POL. ________________________________ From: Bernice Frederick To: "Webster, Thomas S." ; "'histonet@lists.utsouthwestern.edu'" Sent: Monday, November 5, 2012 11:22 AM Subject: RE: [Histonet] Devasting news on 88305TC component Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies......Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 10:11 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame "client billing" the most. The government is tired of being the "pull through" business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information.? If you are not the intended recipient, please contact the sender by reply e-mail immediately.? Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From lblazek <@t> digestivespecialists.com Mon Nov 5 14:48:10 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Nov 5 14:49:39 2012 Subject: [Histonet] slide and cassette labeler In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391643405187@IBMB7Exchange.digestivespecialists.com> Mary, Look into Primera Technologies. They have a slide printer that would be ideal for your situation. The distributer for them is Creative Waste Solutions http://cwsincorp.com/ Talk to Rex. We've had the slide printer for a while now and really like it. Rumor has it that they may be coming out with a cassette printer in the near future too. http://www.primerahealthcare.com/signature-slide-printer.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Fontaine Sent: Monday, November 05, 2012 2:50 PM To: histonet Cc: Daniel Jones Subject: [Histonet] slide and cassette labeler Our hospital will be looking into a cassette =d slide labeler. We are a small lab with a volume of about 4000 surgicals= would appreciate any suggestions on the company and model that has work? will for others. This may be a stand alone saystem that is not hook up t=he hospital system .Thank you [1]mary.fontaine@vrh.org References 1. 3D"mailto:mary.fontaine@vrh.org" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Nov 5 14:56:27 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Nov 5 14:56:28 2012 Subject: [Histonet] IHC negative controls In-Reply-To: <761E2B5697F795489C8710BCC72141FF029D2F@ex07.net.ucsf.edu> References: , <77DD817201982748BC67D7960F2F76AF012798@UWHC-MBX12.uwhis.hosp.wisc.edu> <761E2B5697F795489C8710BCC72141FF029D2F@ex07.net.ucsf.edu> Message-ID: The thing is in my opinion that having the backing of CAP on this issue is a good argument to make to CLIA for why you are not doing negatives for polymer based detection IHC work, at least you will have some documentation to cover your decision. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, October 26, 2012 9:45 AM To: Glen Dawson; histonet Subject: RE: [Histonet] IHC negative controls " Can anyone tell me if JHACO & CLIA are deferring to CAP's judgment that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure." Short answer: Don't bet the farm on it. Each enforces CLIA regulations but have different methods of doing so. There is no reason to think that JC or CAP will defer to the other in any particular situation. They really don't have anything to do with one another. My experience is that CAP is more lab-method oriented while JC is more total-process (patient admission to final result ) oriented. Long answer: Let's clarify this. CLIA is the law administered by the Centers for Medicare and Medicaid Services (CMS). The Joint Commission and CAP are two different, independent accrediting agencies deemed by CMS to enforce the CLIA regulations. CMS/ CLIA does not "defer" to CAP or JC, rather CMS deems JC and CAP to be their agent to accredit laboratories according to the CLIA law. CAP and JC cannot enforce anything without CMS/CLIA approval. The fact that CAP allows labs to leave out negative controls in certain situations may be approved by CMS/ CLIA regulators, but it does not follow that CLIA or JC inspectors will follow the same rational. JC is totally independent and can make their own interpretation of the CLIA regulations, which CMS can approve, even if they are different than what CAP allows, as long as it is within the scope of the CLIA regulations. JC can simply interpret it differently and require negative controls (I don't know if that is the case; I haven't yet looked over the new checklist this year). Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Friday, October 26, 2012 6:45 AM To: histonet Subject: [Histonet] IHC negative controls All, Can anyone tell me if JHACO & CLIA are deferring to CAP's judgement that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure. Thank-you in advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Janesville, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Nov 5 14:59:45 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Nov 5 14:59:49 2012 Subject: [Histonet] cutting bone with metal In-Reply-To: <6797F14E80314F79B7B65D7F6967680E@HP2010> References: <6797F14E80314F79B7B65D7F6967680E@HP2010> Message-ID: <187F89E1251D44ACB6F176D5AE5048D2@DESKTOP3> Bone with metal implants will require ground sections prepared from methyl methacrylate embedded samples, microtome sections even using tungsten carbide knives and MMA embedding cannot usually be done in my experience. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, October 26, 2012 3:16 AM To: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] cutting bone with metal Talk with Jack Ratliff, Chair of the NSH Hard Tissue Committee. Jack L. Ratliff 615-236-4901 ratliffjack@gmail.com The answer is Yes, histologic sections can be made, but need plastic resins (methyl methracylate or glycol methacrylate or something similar) and special microtomes and knives. If the researcher's lab doesn't do this technique, Jack can let him know who does, and the tissue can be sent out to the specialty lab. Paraffin blocks on regular histology microtomes won't cut it - literally and figuratively. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are my own, and do not reflect on Beaumont Hospital. -----Original Message----- From: Jennifer MacDonald Sent: Thursday, October 25, 2012 11:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting bone with metal I have been asked the following question. I do not have an answer and was hoping someone in the Histonet community did. Thanks. There is a researcher who is doing orthopedic procedures on broken rat tibias. The researcher is repairing the tibias with metal rods or plates.not sure which (and the doctor isn't sure what kind of metal either). The researcher wants to know if it is possible to make histologic sections of the repaired tibias with the metal intact _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twebster <@t> CRH.org Mon Nov 5 15:01:26 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Mon Nov 5 15:01:34 2012 Subject: [Histonet] Devasting news on 88305TC component Message-ID: <7207186ED68FB542803CAF1CE6E82FF8032FD7@exmb1.crh.org> I wish there was a way to put a positive spin on this but I can't think of any. We can only hope it kills off client billing somehow. Whomever the "stakeholder" was that told CMS a typical 88305 costs 18 bucks, I'd love to know how he/she came up with that number. It's insultingly low. http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20proposed%20rule%208-30-11_3.pdf I believe whoever it was had the goal to stop the proliferation of POLs. Wouldn't surprise me if they worked for a large national lab that had lost a lot of business to POLs. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From Timothy.Morken <@t> ucsfmedctr.org Mon Nov 5 15:10:13 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Nov 5 15:10:26 2012 Subject: [Histonet] IHC negative controls In-Reply-To: References: , <77DD817201982748BC67D7960F2F76AF012798@UWHC-MBX12.uwhis.hosp.wisc.edu> <761E2B5697F795489C8710BCC72141FF029D2F@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF02B538@ex07.net.ucsf.edu> Could be, but my point, maybe not clear in my answer, is that you should check with your accrediting agency before an inspection and not assume that they will follow what CAP has said it will do. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, November 05, 2012 12:56 PM To: Morken, Timothy; 'Glen Dawson'; 'histonet' Subject: RE: [Histonet] IHC negative controls The thing is in my opinion that having the backing of CAP on this issue is a good argument to make to CLIA for why you are not doing negatives for polymer based detection IHC work, at least you will have some documentation to cover your decision. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, October 26, 2012 9:45 AM To: Glen Dawson; histonet Subject: RE: [Histonet] IHC negative controls " Can anyone tell me if JHACO & CLIA are deferring to CAP's judgment that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure." Short answer: Don't bet the farm on it. Each enforces CLIA regulations but have different methods of doing so. There is no reason to think that JC or CAP will defer to the other in any particular situation. They really don't have anything to do with one another. My experience is that CAP is more lab-method oriented while JC is more total-process (patient admission to final result ) oriented. Long answer: Let's clarify this. CLIA is the law administered by the Centers for Medicare and Medicaid Services (CMS). The Joint Commission and CAP are two different, independent accrediting agencies deemed by CMS to enforce the CLIA regulations. CMS/ CLIA does not "defer" to CAP or JC, rather CMS deems JC and CAP to be their agent to accredit laboratories according to the CLIA law. CAP and JC cannot enforce anything without CMS/CLIA approval. The fact that CAP allows labs to leave out negative controls in certain situations may be approved by CMS/ CLIA regulators, but it does not follow that CLIA or JC inspectors will follow the same rational. JC is totally independent and can make their own interpretation of the CLIA regulations, which CMS can approve, even if they are different than what CAP allows, as long as it is within the scope of the CLIA regulations. JC can simply interpret it differently and require negative controls (I don't know if that is the case; I haven't yet looked over the new checklist this year). Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Friday, October 26, 2012 6:45 AM To: histonet Subject: [Histonet] IHC negative controls All, Can anyone tell me if JHACO & CLIA are deferring to CAP's judgement that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure. Thank-you in advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Janesville, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Nov 5 16:08:18 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Nov 5 16:08:20 2012 Subject: [Histonet] IHC negative controls In-Reply-To: <761E2B5697F795489C8710BCC72141FF02B538@ex07.net.ucsf.edu> References: , <77DD817201982748BC67D7960F2F76AF012798@UWHC-MBX12.uwhis.hosp.wisc.edu> <761E2B5697F795489C8710BCC72141FF029D2F@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF02B538@ex07.net.ucsf.edu> Message-ID: So true! Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Monday, November 05, 2012 2:10 PM To: Patsy Ruegg; 'Glen Dawson'; 'histonet' Subject: RE: [Histonet] IHC negative controls Could be, but my point, maybe not clear in my answer, is that you should check with your accrediting agency before an inspection and not assume that they will follow what CAP has said it will do. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, November 05, 2012 12:56 PM To: Morken, Timothy; 'Glen Dawson'; 'histonet' Subject: RE: [Histonet] IHC negative controls The thing is in my opinion that having the backing of CAP on this issue is a good argument to make to CLIA for why you are not doing negatives for polymer based detection IHC work, at least you will have some documentation to cover your decision. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, October 26, 2012 9:45 AM To: Glen Dawson; histonet Subject: RE: [Histonet] IHC negative controls " Can anyone tell me if JHACO & CLIA are deferring to CAP's judgment that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure." Short answer: Don't bet the farm on it. Each enforces CLIA regulations but have different methods of doing so. There is no reason to think that JC or CAP will defer to the other in any particular situation. They really don't have anything to do with one another. My experience is that CAP is more lab-method oriented while JC is more total-process (patient admission to final result ) oriented. Long answer: Let's clarify this. CLIA is the law administered by the Centers for Medicare and Medicaid Services (CMS). The Joint Commission and CAP are two different, independent accrediting agencies deemed by CMS to enforce the CLIA regulations. CMS/ CLIA does not "defer" to CAP or JC, rather CMS deems JC and CAP to be their agent to accredit laboratories according to the CLIA law. CAP and JC cannot enforce anything without CMS/CLIA approval. The fact that CAP allows labs to leave out negative controls in certain situations may be approved by CMS/ CLIA regulators, but it does not follow that CLIA or JC inspectors will follow the same rational. JC is totally independent and can make their own interpretation of the CLIA regulations, which CMS can approve, even if they are different than what CAP allows, as long as it is within the scope of the CLIA regulations. JC can simply interpret it differently and require negative controls (I don't know if that is the case; I haven't yet looked over the new checklist this year). Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Friday, October 26, 2012 6:45 AM To: histonet Subject: [Histonet] IHC negative controls All, Can anyone tell me if JHACO & CLIA are deferring to CAP's judgement that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure. Thank-you in advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Janesville, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From delsuec <@t> gmail.com Mon Nov 5 17:15:56 2012 From: delsuec <@t> gmail.com (Deloris Carter) Date: Mon Nov 5 17:16:02 2012 Subject: [Histonet] Background staining on H pylori Message-ID: Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) From jnocito <@t> satx.rr.com Mon Nov 5 17:58:55 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Nov 5 18:03:48 2012 Subject: [Histonet] Background staining on H pylori In-Reply-To: References: Message-ID: <01dc01cdbbb1$83c05d40$8b4117c0$@rr.com> It might be the antibody itself. We are seeing the same problem. My doctor also thinks that our antibody is picking up normal flora also. Now, when I QC the slides I have to go by morphology. We have a couple of reps coming by to boast about their HP. Let you know how that works out. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Nov 5 18:09:15 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Nov 5 18:14:11 2012 Subject: [Histonet] Devasting news on 88305TC component In-Reply-To: <1351877189.5173.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <76E5F76D-C27C-4BF8-9167-D1A172DB0BFE@gmail.com> <1351877189.5173.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <01e901cdbbb2$f6120000$e2360000$@rr.com> I was working with a dermpath, who's friend is a dermatologist setting up their lab. They stopped dead in their tracks. I mean 88305s would be their bread and butter. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, November 02, 2012 12:26 PM To: Nails, Felton; 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component EXACTLY SO! POLs started to offer "better?prices" to interested colleagues and by doing so work started to "drain" into POLs with the pointed out result = less work for Reference labs. Do you think that large ref. labs like Quest or Lab.Corp were going to "take that drainage" sitting on their hands? Sure not! They are business with billions at stake and money to lobby. This is the result. Blame the power of the?lobbyists! Policies and?even sometimes?democracy is tainted by big money. Tell that to the Supreme Court that has ruled that a large company is a?"person".? Ren? J. ________________________________ From: "Nails, Felton" To: 'Jesus Ellin' ; 'Cristi Rigazio' ; Brendal Finlay Cc: "histonet@lists.utsouthwestern.edu" ; "Webster, Thomas S." Sent: Friday, November 2, 2012 1:01 PM Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?!? Seriously!? 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class!? Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" wrote: > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr > > > Brendal Finlay, HT (ASCP) > Medical Center Clinic > brendal.finlay@medicalcenterclinic.com > 850.474.8758 > http://medicalcenterclinic.com > -----Original message----- > From: Davide Costanzo pathlocums@gmail.com > Date: Fri, 02 Nov 2012 10:09:18 -0500 > To: "Webster, Thomas S." twebster@crh.org > Subject: Re: [Histonet] Devasting news on 88305TCcomponent > > That is devastating! Do you have a link to this information? > > Sent from my iPhone > > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote: > >> Devastating I meant >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use > of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact > the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana > 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? 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If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Nov 5 21:02:51 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Nov 5 21:02:55 2012 Subject: [Histonet] cost per H&E Message-ID: <1352170971.85241.YahooMailNeo@web39403.mail.mud.yahoo.com> Depending on how I dice up labor and materials, I come up with a cost per slide (H&E), from receipt of specimen to the slide in the Path's hands ranging everywhere from $6 to $9/ slide.? ? HELP?? How do you distill your cost per slide?? ? Thanks, Cheryl? From joelleweaver <@t> hotmail.com Tue Nov 6 01:54:32 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 6 01:54:38 2012 Subject: AW: [Histonet] overfixation with formalin C.Fox pdf article In-Reply-To: <201211042223360593.00193DF9@smtp.montevideo.com.uy> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at>, <12A4DAFC2FEBB84B8DED5F5E9201B4E917C30B8242@UTHCMS1.uthouston.edu>, <000e01cdba64$3629fdf0$a27df9d0$@gmx.at>, , <201211042223360593.00193DF9@smtp.montevideo.com.uy> Message-ID: Hi everyone- A number of people wanted a copy of the Fox article. Hopefully anyone who emailed me directly got their pdf since I don't think the histonet accepts group attachments. In case anyone else wants this great reference, I posted it on my blog in a post hopefully viewable at the following URL http://histologyconnection.com/?attachment_id=201 Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sun, 4 Nov 2012 22:23:36 -0300 > From: latecor@montevideo.com.uy > To: Histonet@lists.utsouthwestern.edu > Subject: RE: AW: [Histonet] overfixation with formalin C.Fox pdf article > CC: Histonet@lists.utsouthwestern.edu > > > > > On 04/11/2012 at 07:01 p.m. joelle weaver wrote: > > I will be pleased if someone could send to me a pdf copy of C.Fox article. > Thanks in advance, > Carlos Defeo > Histotechnologist > Montevideo,Uruguay > > > > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jluis.palazon <@t> icman.csic.es Tue Nov 6 02:27:18 2012 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Tue Nov 6 02:27:52 2012 Subject: AW: [Histonet] overfixation with formalin C.Fox pdf article Message-ID: <549869620.14518.1352190438310.JavaMail.tomcat@cedric> Thanks Joelle. I took it from your block. Regards, Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, C?diz, Espa?a email: jluis.palazon@icman.csic.es; jose.palazon@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 El dia 06 nov 2012 08:54, joelle weaver escribi?: > Hi everyone- A number of people wanted a copy of the Fox article. Hopefully anyone who emailed me directly got their pdf since I don't think the histonet accepts group attachments. In case anyone else wants this great reference, I posted it on my blog in a post hopefully viewable at the following URL http://histologyconnection.com/?attachment_id=201 [ http://histologyconnection.com/?attachment_id=201 ] > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > Date: Sun, 4 Nov 2012 22:23:36 -0300 > >> From: latecor@montevideo.com.uy >> To: Histonet@lists.utsouthwestern.edu >> Subject: RE: AW: [Histonet] overfixation with formalin C.Fox pdf article >> CC: Histonet@lists.utsouthwestern.edu >> >> >> >> >> On 04/11/2012 at 07:01 p.m. joelle weaver wrote: >> >> I will be pleased if someone could send to me a pdf copy of C.Fox article. >> Thanks in advance, >> Carlos Defeo >> Histotechnologist >> Montevideo,Uruguay >> >> >> >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] >>>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] >>> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] > From LSebree <@t> uwhealth.org Tue Nov 6 07:31:18 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Nov 6 07:31:28 2012 Subject: [Histonet] Background staining on H pylori In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0140EC@UWHC-MBX12.uwhis.hosp.wisc.edu> Deloris, We recently had what we think was a contaminated dispenser of HP from VMS. It had brown particulate matter scattered over the slide which the docs said was not bugs. Received a new dispenser from VMS and have had no problem since. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Tue Nov 6 08:25:45 2012 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Nov 6 08:25:53 2012 Subject: [Histonet] Looking at cryostats Message-ID: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From thiggins <@t> cddmedical.com Tue Nov 6 08:27:10 2012 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Tue Nov 6 08:27:18 2012 Subject: Subject: [Histonet] Background staining on H pylori In-Reply-To: <20121106133522.123686E60BD@barracuda.crvinc.net> References: <20121106133522.123686E60BD@barracuda.crvinc.net> Message-ID: <1FC29FDEC8AD4192877047691E0725F0@cdd.loc> Hey Deloris, I would have the dispensing nozzles on the XT checked, could be it is not getting rinsed properly. Had that issue with my Ultra not to long ago. No warnings or errors showed up, just a clogged nozzle tip. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 108, Issue 7 **************************************** From rjbuesa <@t> yahoo.com Tue Nov 6 08:34:21 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 6 08:34:29 2012 Subject: [Histonet] Looking at cryostats In-Reply-To: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> References: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> Message-ID: <1352212461.21616.YahooMailNeo@web163104.mail.bf1.yahoo.com> Not really! For me the Leica is the best. Ren? J. ________________________________ From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Tuesday, November 6, 2012 9:25 AM Subject: [Histonet] Looking at cryostats What's good out there these days?? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Nov 6 08:35:21 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Nov 6 08:35:29 2012 Subject: [Histonet] Looking at cryostats In-Reply-To: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> References: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA328A55BB26@Mail2Node1.ad.uams.edu> The CM3050s is the best in my opinion for research or heavy duty frozen sectioning and it can work with the Cryo Jane system or similar if needed. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Tuesday, November 06, 2012 8:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Tue Nov 6 08:37:09 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 6 08:37:16 2012 Subject: [Histonet] cost per H&E In-Reply-To: <1352170971.85241.YahooMailNeo@web39403.mail.mud.yahoo.com> References: <1352170971.85241.YahooMailNeo@web39403.mail.mud.yahoo.com> Message-ID: <1352212629.10960.YahooMailNeo@web163102.mail.bf1.yahoo.com> That is quite acceptable cost. Under separate cover I am sending my costs. Ren? J. ________________________________ From: Cheryl To: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 5, 2012 10:02 PM Subject: [Histonet] cost per H&E Depending on how I dice up labor and materials, I come up with a cost per slide (H&E), from receipt of specimen to the slide in the Path's hands ranging everywhere from $6 to $9/ slide.? ? HELP?? How do you distill your cost per slide?? ? Thanks, Cheryl? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Tue Nov 6 09:19:56 2012 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Nov 6 09:20:08 2012 Subject: Fw: [Histonet] Cytology in Jasper, Indiana Message-ID: Good Morning Histonetters, We are currently looking for a registered Cytotechnologist to work in a Regional hospital doing all aspects of cytology. The position is full time requiring someone with a clinical Cytology background with processing, screening, assisting on FNA's and learning Cervista HPV HR and Probetec for GC/CT. This is a challenging position with all aspects of cytology and molecular as well. Please contact me directly by e-mail or check mhhcc.org website under careers to apply through the system. Thank You, Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) sbaldwin@mhhcc.org Memorial Hospital and Health Care Center 800 West 9th Str. Jasper, In 47546 Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Nov 6 09:37:45 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Nov 6 09:37:55 2012 Subject: [Histonet] Looking at cryostats In-Reply-To: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> References: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34CE5@EXCHMB01.isis.local> Leica!!!! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Tuesday, November 06, 2012 6:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Nov 6 10:08:25 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Nov 6 10:08:38 2012 Subject: [Histonet] Looking at cryostats In-Reply-To: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> References: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF02B6F8@ex07.net.ucsf.edu> Thermo NX70 is very nice. In fact everyone fights to use it rather than the old CM3050 we have... We use them for frozen sections for kidney, skin IF and muscle histochemistry. Lots and lots of frozens... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Tuesday, November 06, 2012 6:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Nov 6 10:13:54 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Nov 6 10:13:59 2012 Subject: [Histonet] Looking at cryostats In-Reply-To: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> References: <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719BE1353F3@EVS1.archildrens.org> Leica! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Tuesday, November 06, 2012 8:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tajibade <@t> echd.org Tue Nov 6 12:02:29 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Tue Nov 6 12:02:43 2012 Subject: [Histonet] Job Opportunity!!! Message-ID: Medical Center Hospital is a premier center and we currently have opening for Histotechnicain/Histotechnologist in our Department. This is a full time opportunity that will rotate through all area of histology routine procedures, special stains and IHC.Please if interested send your resume to me at Email;tajibade@echd.org Fax no: 432-640 2303 Tel: 432-6402348. Tunde Ajibade BS, HTL(ASCP),QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel: 432-640-2348 Fax:432-640-2303 CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From Wanda.Smith <@t> HCAhealthcare.com Tue Nov 6 12:03:10 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Nov 6 12:03:22 2012 Subject: [Histonet] SC Society of Histotechnology Meeting This Weekend Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27CB537239@NADCWPMSGCMS03.hca.corpad.net> Good afternoon Everyone!!!! This is just to let you know the SCSHT Meeting will be this weekend November 9-10, 2012 at the beachfront Landmark Resort in Myrtle Beach, SC. 7contact hours available and workshops include "Lean Workflow in Histology: Error Reduction" "Tissue Processing from the Beginning" and "Histohints to Happiness"! You can call or email me for a brochure and registration information. HOPE TO SEE YOU THERE!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From LRaff <@t> uropartners.com Tue Nov 6 13:19:46 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Tue Nov 6 13:19:50 2012 Subject: FW: [Histonet] Devasting news on 88305TC component--Where the $18 came from Message-ID: Sent: Tuesday, November 06, 2012 12:35 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: RE: [Histonet] Devasting news on 88305TC component--Where the $18 came from The $18.00 figure came from an article published in Archives of Pathology and Lab Medicine: Pathology Economic Model Tool A Novel Approach to Workflow and Budget Cost Analysis in an Anatomic Pathology Laboratory David Muirhead, BSc; Patricia Aoun, MD, MPH; Michael Powell, MS, FASHP; Flemming Juncker, MBA; Jens Mollerup, MSc, PhD (Arch Pathol Lab Med. 2010;134:1164-1169) Thanks to Joe Plandowski of IOL for providing me with the reference. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 3:01 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component I wish there was a way to put a positive spin on this but I can't think of any. We can only hope it kills off client billing somehow. Whomever the "stakeholder" was that told CMS a typical 88305 costs 18 bucks, I'd love to know how he/she came up with that number. It's insultingly low. http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20p roposed%20rule%208-30-11_3.pdf I believe whoever it was had the goal to stop the proliferation of POLs. Wouldn't surprise me if they worked for a large national lab that had lost a lot of business to POLs. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amylee779 <@t> yahoo.com Tue Nov 6 13:41:26 2012 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Tue Nov 6 13:41:30 2012 Subject: [Histonet] How to fix frozen section? Message-ID: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> Hello, ? I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some?use only alcohol. Could you please recommend a fixation method for me? ? ? Thanks, ? Amy From LSebree <@t> uwhealth.org Tue Nov 6 13:44:58 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Nov 6 13:45:05 2012 Subject: [Histonet] How to fix frozen section? In-Reply-To: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> References: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> Message-ID: <77DD817201982748BC67D7960F2F76AF014251@UWHC-MBX12.uwhis.hosp.wisc.edu> It depends upon what marker you're staining for. We fix our renal biopsies for C4d in acetone for 10". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 1:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello, ? I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some?use only alcohol. Could you please recommend a fixation method for me? ? ? Thanks, ? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pam <@t> dlcjax.com Tue Nov 6 13:45:19 2012 From: pam <@t> dlcjax.com (Pam Mathews) Date: Tue Nov 6 13:45:23 2012 Subject: [Histonet] Histologist Message-ID: <2339.208.62.167.196.1352231119.squirrel@webmail.realpages.com> Busy Derm Practice looking for an experienced Histologist to run Derm Path lab. Full or Part time. Must be able to perform all routine histology duties soley. Embedding, grossing, accession, coverslipping, microtonomy, record keeping, H&E stain and special stains. Candidate needs to be familiar with OSHA and CLIA standards. Must be able to update and maintain all manuals. Mohs experience preferred. Pam Mathews, CDC Dermatology and Laser Center Office Manager 904-276-4500 Office 904-276-4160 Fax 904-945-6845 Cell From brett_connolly <@t> merck.com Tue Nov 6 14:08:39 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Nov 6 14:09:06 2012 Subject: [Histonet] How to fix frozen section? In-Reply-To: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> References: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> Message-ID: Amy, We use ice-cold acetone/ethanol (3:1) for 10'...works for all of our antigens so far. Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 2:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello, ? I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some?use only alcohol. Could you please recommend a fixation method for me? ? ? Thanks, ? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. 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From JEllin <@t> yumaregional.org Tue Nov 6 14:11:06 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Nov 6 14:11:16 2012 Subject: [Histonet] Devasting news on 88305TC component--Where the $18 came from In-Reply-To: References: Message-ID: <01935B86-D23C-49F3-9A30-098F3046C4F3@yumaregional.org> Well in this article it does have differences, especially when talking about usage of technology,, but non the less it does expose our state of being within Anatomic Pathology,, ,, what are peoples thought on this Sent from my iPad On Nov 6, 2012, at 12:21 PM, "Lester Raff MD" wrote: > Sent: Tuesday, November 06, 2012 12:35 PM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Devasting news on 88305TC component--Where the > $18 came from > > The $18.00 figure came from an article published in Archives of > Pathology and Lab Medicine: > > Pathology Economic Model Tool > A Novel Approach to Workflow and Budget Cost Analysis in an Anatomic > Pathology Laboratory > David Muirhead, BSc; Patricia Aoun, MD, MPH; Michael Powell, MS, FASHP; > Flemming Juncker, MBA; Jens Mollerup, MSc, PhD > > > (Arch Pathol Lab Med. 2010;134:1164-1169) > > Thanks to Joe Plandowski of IOL for providing me with the reference. > > Lester J. Raff, MD > Medical Director > UroPartners Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel 708.486.0076 > Fax 708.492.0203 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, > Thomas S. > Sent: Monday, November 05, 2012 3:01 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Devasting news on 88305TC component > > I wish there was a way to put a positive spin on this but I can't think > of any. We can only hope it kills off client billing somehow. > > Whomever the "stakeholder" was that told CMS a typical 88305 costs 18 > bucks, I'd love to know how he/she came up with that number. It's > insultingly low. > > http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20p > roposed%20rule%208-30-11_3.pdf > > I believe whoever it was had the goal to stop the proliferation of POLs. > Wouldn't surprise me if they worked for a large national lab that had > lost a lot of business to POLs. > > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From madeleinehuey <@t> gmail.com Tue Nov 6 14:22:51 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Tue Nov 6 14:22:56 2012 Subject: [Histonet] Re: Histonet Digest, Vol 108, Issue 8 In-Reply-To: <5099504e.2870b60a.5b47.556eSMTPIN_ADDED@mx.google.com> References: <5099504e.2870b60a.5b47.556eSMTPIN_ADDED@mx.google.com> Message-ID: Leica Cryostat CM1850 UV for me! Used them for more then 20 years and still prefer them.:) Madeleine Huey On Tue, Nov 6, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Looking at cryostats (kgrobert@rci.rutgers.edu) > 2. Subject: [Histonet] Background staining on H pylori (Tim Higgins) > 3. Re: Looking at cryostats (Rene J Buesa) > 4. RE: Looking at cryostats (Marcum, Pamela A) > 5. Re: cost per H&E (Rene J Buesa) > 6. Fw: [Histonet] Cytology in Jasper, Indiana > (Sara Baldwin/mhhcc.org) > 7. RE: Looking at cryostats (Bea DeBrosse-Serra) > 8. RE: Looking at cryostats (Morken, Timothy) > 9. RE: Looking at cryostats (Horn, Hazel V) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 6 Nov 2012 09:25:45 -0500 (EST) > From: kgrobert@rci.rutgers.edu > Subject: [Histonet] Looking at cryostats > To: "histonet" > Message-ID: > <2ccd020a326d6f64ee276b51bdbcb637.squirrel@webmail.rci.rutgers.edu> > Content-Type: text/plain;charset=iso-8859-1 > > What's good out there these days? I am currently looking at the Leica > CM3050; are there other brands that I should consider? > > Thanks so much, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > > > ------------------------------ > > Message: 2 > Date: Tue, 6 Nov 2012 08:27:10 -0600 > From: "Tim Higgins" > Subject: Subject: [Histonet] Background staining on H pylori > To: > Message-ID: <1FC29FDEC8AD4192877047691E0725F0@cdd.loc> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hey Deloris, > > I would have the dispensing nozzles on the XT checked, could be it is not > getting rinsed properly. Had that issue with my Ultra not to long ago. No > warnings or errors showed up, just a clogged nozzle tip. > > Tim > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris > Carter > Sent: Monday, November 05, 2012 5:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Background staining on H pylori > > Hi, > I'm getting a lot of background staining on my HP's. We use a Ventana > BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, > and I'm not sure why. We use Hollande's on our GI biopsies, and run them on > a shorter run. Nothing has changed in the processing of the specimens, or > the IHC procedure. The antibody dispenser is a newer lot, but not brand > new, as we get the larger size due to the high volume of HP's we run. Any > ideas? > > Deloris Carter HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 108, Issue 7 > **************************************** > > > > > ------------------------------ > > Message: 3 > Date: Tue, 6 Nov 2012 06:34:21 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Looking at cryostats > To: "kgrobert@rci.rutgers.edu" , histonet > > Message-ID: > <1352212461.21616.YahooMailNeo@web163104.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Not really! For me the Leica is the best. > Ren? J. > > > ________________________________ > From: "kgrobert@rci.rutgers.edu" > To: histonet > Sent: Tuesday, November 6, 2012 9:25 AM > Subject: [Histonet] Looking at cryostats > > What's good out there these days? I am currently looking at the Leica > CM3050; are there other brands that I should consider? > > Thanks so much, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 4 > Date: Tue, 6 Nov 2012 14:35:21 +0000 > From: "Marcum, Pamela A" > Subject: RE: [Histonet] Looking at cryostats > To: "'kgrobert@rci.rutgers.edu'" , histonet > > Message-ID: > <41D3A1AF6FEF0643BDC89E0516A6EA328A55BB26@Mail2Node1.ad.uams.edu> > Content-Type: text/plain; charset="us-ascii" > > The CM3050s is the best in my opinion for research or heavy duty frozen sectioning and it can work with the Cryo Jane system or similar if needed. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu > Sent: Tuesday, November 06, 2012 8:26 AM > To: histonet > Subject: [Histonet] Looking at cryostats > > What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? > > Thanks so much, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, > use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > > > > > ------------------------------ > > Message: 5 > Date: Tue, 6 Nov 2012 06:37:09 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] cost per H&E > To: Cheryl , "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1352212629.10960.YahooMailNeo@web163102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That is quite acceptable cost. > Under separate cover I am sending my costs. > Ren? J. > > > ________________________________ > From: Cheryl > To: "histonet@lists.utsouthwestern.edu" > Sent: Monday, November 5, 2012 10:02 PM > Subject: [Histonet] cost per H&E > > > > Depending on how I dice up labor and materials, I come up with a cost per slide (H&E), from receipt of specimen to the slide in the Path's hands ranging everywhere from $6 to $9/ slide. > > HELP? How do you distill your cost per slide?? > > Thanks, > > Cheryl > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 6 > Date: Tue, 6 Nov 2012 10:19:56 -0500 > From: "Sara Baldwin/mhhcc.org" > Subject: Fw: [Histonet] Cytology in Jasper, Indiana > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Good Morning Histonetters, > > We are currently looking for a registered Cytotechnologist to work in a Regional hospital doing all aspects of cytology. The position is full time requiring someone with a clinical Cytology background with processing, screening, assisting on FNA's and learning Cervista HPV HR and Probetec for GC/CT. This is a challenging position with all aspects of cytology and molecular as well. > > > Please contact me directly by e-mail or check mhhcc.org website under careers to apply through the system. > > Thank You, > > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > sbaldwin@mhhcc.org > Memorial Hospital and Health Care Center > 800 West 9th Str. > Jasper, In 47546 > Ph 812-996-0210, 0216, Fax 812-996-0232, > Pager 812-481-0897 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Tue, 6 Nov 2012 07:37:45 -0800 > From: Bea DeBrosse-Serra > Subject: RE: [Histonet] Looking at cryostats > To: "'kgrobert@rci.rutgers.edu'" , histonet > > Message-ID: > <493CAA64F203E14E8823737B9EE0E25F092CE34CE5@EXCHMB01.isis.local> > Content-Type: text/plain; charset="us-ascii" > > Leica!!!! > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu > Sent: Tuesday, November 06, 2012 6:26 AM > To: histonet > Subject: [Histonet] Looking at cryostats > > What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? > > Thanks so much, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 6 Nov 2012 16:08:25 +0000 > From: "Morken, Timothy" > Subject: RE: [Histonet] Looking at cryostats > To: histonet > Message-ID: <761E2B5697F795489C8710BCC72141FF02B6F8@ex07.net.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Thermo NX70 is very nice. In fact everyone fights to use it rather than the old CM3050 we have... > > We use them for frozen sections for kidney, skin IF and muscle histochemistry. Lots and lots of frozens... > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu > Sent: Tuesday, November 06, 2012 6:26 AM > To: histonet > Subject: [Histonet] Looking at cryostats > > What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? > > Thanks so much, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Tue, 6 Nov 2012 10:13:54 -0600 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Looking at cryostats > To: "'kgrobert@rci.rutgers.edu'" , histonet > > Message-ID: > <25A4DE08332B19499904459F00AAACB719BE1353F3@EVS1.archildrens.org> > Content-Type: text/plain; charset="us-ascii" > > Leica! > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu > Sent: Tuesday, November 06, 2012 8:26 AM > To: histonet > Subject: [Histonet] Looking at cryostats > > What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? > > Thanks so much, > Kathleen > > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 108, Issue 8 > **************************************** From rjbuesa <@t> yahoo.com Tue Nov 6 14:58:13 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 6 14:58:17 2012 Subject: [Histonet] How to fix frozen section? In-Reply-To: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> References: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> Message-ID: <1352235493.1466.YahooMailNeo@web163103.mail.bf1.yahoo.com> I used acetone Ren? J. ________________________________ From: Amy Lee To: histonet Sent: Tuesday, November 6, 2012 2:41 PM Subject: [Histonet] How to fix frozen section? Hello, ? I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some?use only alcohol. Could you please recommend a fixation method for me? ? ? Thanks, ? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruhella.arjun <@t> gmail.com Tue Nov 6 15:04:25 2012 From: ruhella.arjun <@t> gmail.com (arjun ruhella) Date: Tue Nov 6 15:04:31 2012 Subject: [Histonet] How to fix frozen section? In-Reply-To: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> References: <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> Message-ID: <71A27E91-9780-448C-B90D-BB6D70E30ADB@gmail.com> i use 1:1 Acetone : alcohol. On Nov 6, 2012, at 2:41 PM, Amy Lee wrote: > Hello, > > I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? > > > Thanks, > > Amy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best, Arjun From Pat.Bell <@t> ucdenver.edu Tue Nov 6 15:44:55 2012 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Tue Nov 6 15:45:00 2012 Subject: [Histonet] CD11b Message-ID: <64DB27005E2FD3439E88502D7A5C9121FC074691E6@CORTEZ.ucdenver.pvt> Hello to Everyone!! :) Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From cforster <@t> umn.edu Tue Nov 6 16:00:10 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Nov 6 16:00:18 2012 Subject: [Histonet] CD11b In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121FC074691E6@CORTEZ.ucdenver.pvt> References: <64DB27005E2FD3439E88502D7A5C9121FC074691E6@CORTEZ.ucdenver.pvt> Message-ID: <5099886A.2070102@umn.edu> Me too!!! C On 11/6/2012 3:44 PM, Bell, Pat wrote: > Hello to Everyone!! :) > > Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) > > Thank you, > Pat > > Pat Bell HT(ASCP) > University of Colorado, Denver > Medical Oncology; MS 8117 > 12801 E 17th Ave. > RC1-S, L18-8402C > Aurora, Co. 80045 > 303-724-6077 > pat.bell@ucdenver.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Wed Nov 7 01:59:02 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 7 01:59:12 2012 Subject: [Histonet] Histologist In-Reply-To: <2339.208.62.167.196.1352231119.squirrel@webmail.realpages.com> References: <2339.208.62.167.196.1352231119.squirrel@webmail.realpages.com> Message-ID: Where is the location please? Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 6 Nov 2012 14:45:19 -0500 > From: pam@dlcjax.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histologist > > Busy Derm Practice looking for an experienced Histologist to run Derm Path > lab. Full or Part time. Must be able to perform all routine histology > duties soley. Embedding, grossing, accession, coverslipping, microtonomy, > record keeping, H&E stain and special stains. Candidate needs to be > familiar with OSHA and CLIA standards. Must be able to update and maintain > all manuals. Mohs experience preferred. > > > > > Pam Mathews, CDC > Dermatology and Laser Center > Office Manager > 904-276-4500 Office > 904-276-4160 Fax > 904-945-6845 Cell > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Nov 7 01:59:50 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 7 01:59:54 2012 Subject: [Histonet] Devasting news on 88305TC component--Where the $18 came from In-Reply-To: References: Message-ID: Does anyone have a link to this article? Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 6 Nov 2012 13:19:46 -0600 > From: LRaff@uropartners.com > To: histonet@lists.utsouthwestern.edu > Subject: FW: [Histonet] Devasting news on 88305TC component--Where the $18 came from > > Sent: Tuesday, November 06, 2012 12:35 PM > To: 'histonet-bounces@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Devasting news on 88305TC component--Where the > $18 came from > > The $18.00 figure came from an article published in Archives of > Pathology and Lab Medicine: > > Pathology Economic Model Tool > A Novel Approach to Workflow and Budget Cost Analysis in an Anatomic > Pathology Laboratory > David Muirhead, BSc; Patricia Aoun, MD, MPH; Michael Powell, MS, FASHP; > Flemming Juncker, MBA; Jens Mollerup, MSc, PhD > > > (Arch Pathol Lab Med. 2010;134:1164-1169) > > Thanks to Joe Plandowski of IOL for providing me with the reference. > > Lester J. Raff, MD > Medical Director > UroPartners Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel 708.486.0076 > Fax 708.492.0203 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, > Thomas S. > Sent: Monday, November 05, 2012 3:01 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Devasting news on 88305TC component > > I wish there was a way to put a positive spin on this but I can't think > of any. We can only hope it kills off client billing somehow. > > Whomever the "stakeholder" was that told CMS a typical 88305 costs 18 > bucks, I'd love to know how he/she came up with that number. It's > insultingly low. > > http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20p > roposed%20rule%208-30-11_3.pdf > > I believe whoever it was had the goal to stop the proliferation of POLs. > Wouldn't surprise me if they worked for a large national lab that had > lost a lot of business to POLs. > > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DianaRip1 <@t> aol.com Wed Nov 7 07:01:49 2012 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Wed Nov 7 07:01:57 2012 Subject: [Histonet] Getting parts for old Leica TP 1050 Message-ID: <2a44f.6e12886b.3dcbb5bd@aol.com> I have an old Leica TP 1050 as a backup machine. Just loved the machine. Thought it was great before Leica decided to make changes in the new model. However, it has had a recent break down possibly a solenoid and something else. My repair guy says he can no longer get parts for it. What is everyone else doing that may still have one of these machines? Thanks so much Diana Ripley From twebster <@t> CRH.org Wed Nov 7 08:09:26 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Wed Nov 7 08:09:35 2012 Subject: [Histonet] Devasting news on 88305TC component--Where the $18 came from Message-ID: <7207186ED68FB542803CAF1CE6E82FF8033142@exmb1.crh.org> Thanks for telling us about this article Dr Raff. Here is a link http://www.archivesofpathology.org/doi/pdf/10.1043/2000-0401-OA.1 CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From jhill <@t> vet.k-state.edu Wed Nov 7 08:13:49 2012 From: jhill <@t> vet.k-state.edu (Jennifer Hill) Date: Wed Nov 7 08:13:57 2012 Subject: [Histonet] Control Tissue Exchange Message-ID: <8AA2173DC209CA438077A832FF98BD7F07B41B40@VETMXHT.ads.vet.k-state.edu> Hello All, I need some help trying to find control tissue for a research project. I need human breast carcinoma. I work in a Veterinary Diagnostic Lab, so I don't have any human tissue, but I can exchange various animal tissues for controls. This would include normal tissue from a variety of species as well as some infectious diseases. Thanks, Jennifer Hill Research Assistant Kansas State University Veterinary Diagnostic Lab From robinsoc <@t> mercyhealth.com Wed Nov 7 08:20:35 2012 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Wed Nov 7 08:20:45 2012 Subject: [Histonet] AFB control Message-ID: <509A19D3020000AF0000B2BC@nodcdmg2.no.trinity-health.org> All, Does anyone have AFB control in human tissue they would be willing to share? I shared fungal controls awhile back with anyone who emailed me....that is, until I ran out. I would be willing to share control material if I have something you need. Thanks. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com From LPaveli1 <@t> hurleymc.com Wed Nov 7 09:29:34 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Nov 7 09:29:50 2012 Subject: [Histonet] AFB control In-Reply-To: <509A19D3020000AF0000B2BC@nodcdmg2.no.trinity-health.org> References: <509A19D3020000AF0000B2BC@nodcdmg2.no.trinity-health.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56889A@EXCHANGEMB1.hmc.hurleymc.com> If you join the Michigan Society of Histotechnologists for $20/year membership, you will receive not only the award winning MikroGraf newsletter, voted #1 by the NSH, but have access to a supply of control blocks as needed for no extra charge! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cynthia Robinson [robinsoc@mercyhealth.com] Sent: Wednesday, November 07, 2012 9:20 AM To: histonet Subject: [Histonet] AFB control All, Does anyone have AFB control in human tissue they would be willing to share? I shared fungal controls awhile back with anyone who emailed me....that is, until I ran out. I would be willing to share control material if I have something you need. Thanks. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Nov 7 09:38:18 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Nov 7 09:38:31 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A7F49AC7D3@EXC-MBX3.cfs.le.ac.uk> I have seen far more damaged/unusable (apart from H@E) tissues through under-fixation than over-fixation..................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 03 November 2012 18:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JHicks <@t> ameripath.com Wed Nov 7 09:45:15 2012 From: JHicks <@t> ameripath.com (Hicks, Jessica L) Date: Wed Nov 7 09:46:02 2012 Subject: [Histonet] Ameripath/Dermpath Diagnostics Employment Opportunities! Message-ID: Ameripath/Dermpath Diagnostics has several employment opportunities across the nation for Histotechs and Histology Lead positions. The positions are located in the following cities: Dallas, Texas Kansas City, Missouri Shelton, Connecticut Phoenix, Arizona We offer competitive compensation and excellent benefits. Please visit our website at www.ameripath.com or contact me at jhicks@ameripath.com for more information. Jessica L. Hicks, PHR, MS AmeriPath | Dermpath Diagnostics | Sr. Staffing Specialist, Employment and Staffing | 2560 N. Shadeland Ave. Suite A | Indianapolis, IN 46219 USA | phone +1.317.351.4118 | fax +1.317.351.4119| mobile +1.317.518.7906 | jhicks@ameripath.com | www.AmeriPath.com | www.DermpathDiagnostics.com Please think about resource conservation before you print this message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Jessica.Vacca <@t> HCAhealthcare.com Wed Nov 7 11:20:47 2012 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Wed Nov 7 11:22:16 2012 Subject: [Histonet] Meaningful Use-Pathology Message-ID: <938D716CD445614ABBB817517557B6F407C6FDEFA9@NADCWPMSGCMS09.hca.corpad.net> How is everyone addressing meeting meaningful use in regards to pathology, when a positive/negative or numeric value is looked at? Objective: Incorporate clinical lab test results into EHR as structured data. Measure: More than 40 percent of all clinical lab test results ordered by the eligible professional during the EHR reporting period whose results are either in a positive/negative or numerical format are incorporated in certified EHR technology as structured data. Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 615-579-0121 o: 615-344-5370 e: Jessica.Vacca@hcahealthcare.com From JEllin <@t> yumaregional.org Wed Nov 7 11:30:46 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Nov 7 11:30:55 2012 Subject: [Histonet] RE: Meaningful Use-Pathology In-Reply-To: <938D716CD445614ABBB817517557B6F407C6FDEFA9@NADCWPMSGCMS09.hca.corpad.net> References: <938D716CD445614ABBB817517557B6F407C6FDEFA9@NADCWPMSGCMS09.hca.corpad.net> Message-ID: Meaningful use is one aspect,, but most of the time you can not do this in EPIC in a fashion that will be able to be searchable and structured. There are other ways of doing this, but most of the time this is a work around -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Wednesday, November 07, 2012 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meaningful Use-Pathology How is everyone addressing meeting meaningful use in regards to pathology, when a positive/negative or numeric value is looked at? Objective: Incorporate clinical lab test results into EHR as structured data. Measure: More than 40 percent of all clinical lab test results ordered by the eligible professional during the EHR reporting period whose results are either in a positive/negative or numerical format are incorporated in certified EHR technology as structured data. Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 615-579-0121 o: 615-344-5370 e: Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From cmiller <@t> physlab.com Wed Nov 7 11:43:42 2012 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Nov 7 11:43:48 2012 Subject: [Histonet] Charges for cutting research case Message-ID: I would like some direction in regards to how to charge a client for cutting multiple slides and levels anywhere between 10 to 50 slides per block of a Technical component case. These would be research cases. Any help directing me would be greatly appreciated. Thank, Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From dgoodwin <@t> rwjuhh.edu Wed Nov 7 12:09:48 2012 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Wed Nov 7 12:10:02 2012 Subject: [Histonet] RE:Charges for cutting research case (Cheri Miller) In-Reply-To: <1352311394.531239@messagescreen2.rwjham.net> References: <1352311394.531239@messagescreen2.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C729D2C44A0D@HAMEXMBA.rwjham.local> We have instituted a price per slide based on a rough cost analysis of how much it takes us to produce a slide, stained and unstained: Reagents, consumables, tech time, clerical time, facility and equipment use. We created an invoice using a template in MS Word which we send along with the slides. Hope this helps. Diana Goodwin Histology Supervisor RWJUHH 609-631-6996 dgoodwin@rwjuhh.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, November 07, 2012 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 108, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Ameripath/Dermpath Diagnostics Employment Opportunities! (Hicks, Jessica L) 2. Meaningful Use-Pathology (Jessica.Vacca@HCAhealthcare.com) 3. RE: Meaningful Use-Pathology (Jesus Ellin) 4. Charges for cutting research case (Cheri Miller) ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 Nov 2012 09:45:15 -0600 From: "Hicks, Jessica L" Subject: [Histonet] Ameripath/Dermpath Diagnostics Employment Opportunities! To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Ameripath/Dermpath Diagnostics has several employment opportunities across the nation for Histotechs and Histology Lead positions. The positions are located in the following cities: Dallas, Texas Kansas City, Missouri Shelton, Connecticut Phoenix, Arizona We offer competitive compensation and excellent benefits. Please visit our website at www.ameripath.com or contact me at jhicks@ameripath.com for more information. Jessica L. Hicks, PHR, MS AmeriPath | Dermpath Diagnostics | Sr. Staffing Specialist, Employment and Staffing | 2560 N. Shadeland Ave. Suite A | Indianapolis, IN 46219 USA | phone +1.317.351.4118 | fax +1.317.351.4119| mobile +1.317.518.7906 | jhicks@ameripath.com | www.AmeriPath.com | www.DermpathDiagnostics.com Please think about resource conservation before you print this message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Wed, 7 Nov 2012 11:20:47 -0600 From: Subject: [Histonet] Meaningful Use-Pathology To: Message-ID: <938D716CD445614ABBB817517557B6F407C6FDEFA9@NADCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" How is everyone addressing meeting meaningful use in regards to pathology, when a positive/negative or numeric value is looked at? Objective: Incorporate clinical lab test results into EHR as structured data. Measure: More than 40 percent of all clinical lab test results ordered by the eligible professional during the EHR reporting period whose results are either in a positive/negative or numerical format are incorporated in certified EHR technology as structured data. Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 615-579-0121 o: 615-344-5370 e: Jessica.Vacca@hcahealthcare.com ------------------------------ Message: 3 Date: Wed, 7 Nov 2012 17:30:46 +0000 From: Jesus Ellin Subject: [Histonet] RE: Meaningful Use-Pathology To: "'Jessica.Vacca@HCAhealthcare.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Meaningful use is one aspect,, but most of the time you can not do this in EPIC in a fashion that will be able to be searchable and structured. There are other ways of doing this, but most of the time this is a work around -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Wednesday, November 07, 2012 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meaningful Use-Pathology How is everyone addressing meeting meaningful use in regards to pathology, when a positive/negative or numeric value is looked at? Objective: Incorporate clinical lab test results into EHR as structured data. Measure: More than 40 percent of all clinical lab test results ordered by the eligible professional during the EHR reporting period whose results are either in a positive/negative or numerical format are incorporated in certified EHR technology as structured data. Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 615-579-0121 o: 615-344-5370 e: Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 4 Date: Wed, 7 Nov 2012 11:43:42 -0600 From: Cheri Miller Subject: [Histonet] Charges for cutting research case To: "histonet-bounces@lists.utsouthwestern.edu" Cc: histonet Message-ID: Content-Type: text/plain; charset="us-ascii" I would like some direction in regards to how to charge a client for cutting multiple slides and levels anywhere between 10 to 50 slides per block of a Technical component case. These would be research cases. Any help directing me would be greatly appreciated. Thank, Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 108, Issue 10 ***************************************** From joelleweaver <@t> hotmail.com Wed Nov 7 12:25:51 2012 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Wed Nov 7 12:26:00 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTG9va2luZyBhdCBjcnlvc3RhdHM=?= Message-ID: Either Leica or Tissue Tek with the UV are my votes Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Bea DeBrosse-Serra" To: "'kgrobert@rci.rutgers.edu'" , "histonet" Subject: [Histonet] Looking at cryostats Date: Tue, Nov 6, 2012 9:37 am Leica!!!! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Tuesday, November 06, 2012 6:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Nov 7 12:31:45 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 7 12:31:49 2012 Subject: [Histonet] Devasting news on 88305TC component--Where the $18 came from In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF8033142@exmb1.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF8033142@exmb1.crh.org> Message-ID: Thanks for the link, I am curious to read this article. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: twebster@CRH.org > To: histonet@lists.utsouthwestern.edu > Date: Wed, 7 Nov 2012 14:09:26 +0000 > Subject: [Histonet] Devasting news on 88305TC component--Where the $18 came from > > Thanks for telling us about this article Dr Raff. > > Here is a link > > > http://www.archivesofpathology.org/doi/pdf/10.1043/2000-0401-OA.1 > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 7 12:46:37 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 7 12:46:39 2012 Subject: [Histonet] Cassette Labeler In-Reply-To: References: <1351347231.27343.YahooMailClassic@web140204.mail.bf1.yahoo.com><6b21d3a7a0e34674310b368e2faf7aa16234e434@localhost><30D679350711B041B577E61FCFC90B6801775798@tbssbs.TBS.local><82C7248978CB50469FD6BA68EBBEFE6708D1CB7F@exchange.propathlab.com> Message-ID: Yea and they welcomed responses by vendors and also wanted to hear from users, this post seems legit to me, I would be glad to have this info. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Monday, October 29, 2012 11:21 AM To: Norm Burnham Cc: histonet@lists.utsouthwestern.edu; Shelly Coker Subject: Re: [Histonet] Cassette Labeler Hi Norm, It sounds like he had something to contribute. I don't think It shouldn't matter where he works. Someone else asked the question. Mark On Mon, Oct 29, 2012 at 10:00 AM, Norm Burnham wrote: > I didn't know that the Histonet site is being used as a medium for vendors > to > advertise their wares? Your thoughts? > Norm Burnham > > ______________________________________________ > Norm Burnham, MBA, MT(ASCP) > Director, Anatomic Laboratory Operations > ProPath - The Leader in Pathology Services > 1355 River Bend Drive > Dallas, TX 75247 > norm.burnham@propath.com > 214.237.1602 Office > 214.237.1802 Fax > 214.709.7127 Cell > > To learn more about ProPath, please visit www.propath.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dustin > Paul > Campbell > Sent: Monday, October 29, 2012 11:54 AM > To: Kaye Ryan; Shelly Coker; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cassette Labeler > > To All, > > TBS offers a compatible cassette and slide labeler that interfaces with > APEASY. We have several accounts using both systems. If addition > information > is needed please contact me directly. dcampbell@trianglebiomedical.com > > Hope this helps, > > > > > Dustin Campbell > Service Technician > > 3014 Croasdaile Drive, Durham NC 27705 > p 919.384.9393 f 919.384.9595 > dcampbell@trianglebiomedical.com > > Visit us online at www.trianglebiomedical.com and follow us on > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan > Sent: Monday, October 29, 2012 9:42 AM > To: Shelly Coker; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cassette Labeler > > Please share that information with me also. > > Thanks, > Kaye > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shelly > Coker > Sent: Saturday, October 27, 2012 10:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cassette Labeler > > Anyone out there with AP Easy software that has a cassette labeler > interfaced > with your LIS software that you would recommend? We are looking at adding > one sometime next year. (PS...while responses from vendors are welcome, I > really want to hear from someone working with the instrument...) > > Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic message is intended to be for the use only of the > named recipient and may contain information that is confidential or > privileged. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution, or use of the contents of this > message is strictly prohibited. If you have received this message in > error or are not the named recipient, please notify us immediately by > contacting the sender at the electronic mail address noted above, and > delete and destroy all copies of this message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 7 13:06:32 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 7 13:06:35 2012 Subject: [Histonet] Dako vs Ventana IHC systems In-Reply-To: <1351524439.26536.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <001e01cdb3b1$616e8a30$244b9e90$@com><77DD817201982748BC67D7960F2F76AF013404@UWHC-MBX12.uwhis.hosp.wisc.edu><731941C266951A47BEF11E5EFAAED9C9127EAD50@nsmvexch01.northside.local><3C2378778400AD448ADA6FD6BDB7CCCC17928CCC@RRMBX03.rrmc.local> <1351524439.26536.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: I second Rene's suggestion, the Leica Bond instrument is my autostainer of choice and I have not had any problems with their service, I find it to be excellent. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 29, 2012 9:27 AM To: Joe W. Walker, Jr.; Terri Brown; Sebree Linda A; Burton, Lynn; Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako vs Ventana IHC systems Now that you are in the market to buy an autostainer, check the "BondMax" originally from BioSystems (Australia) and now distributed by Leica Microsystems. I am sure you will like it. Ren? J. ________________________________ From: "Joe W. Walker, Jr." To: Terri Brown ; Sebree Linda A ; "Burton, Lynn" ; Gloria Tharp ; "histonet@lists.utsouthwestern.edu" Sent: Monday, October 29, 2012 10:38 AM Subject: RE: [Histonet] Dako vs Ventana IHC systems Which Dako and Ventana machines are you all using?? We are in the market for a new IHC stainer and the new Ventana BenckMark Ultra machine looks interesting. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790? F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Monday, October 29, 2012 10:26 AM To: Sebree Linda A; Burton, Lynn; Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems We had Ventana and changed to Dako.? The Dako phone tech support is excellent and we never wait long for in house service.? Our pathologists love the turnaround time with the open system and the stain quality. Terri H. Brown, HT (ASCP) Pathology Laboratory Manager Northside Hospital? Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, October 26, 2012 4:52 PM To: 'Burton, Lynn'; Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems I concur with Lynn; we've been dealing with Ventana 19 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Friday, October 26, 2012 3:47 PM To: Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems We have been dealing with Ventana for at least 10 years with very good service and reliability. Their phone tech support is excellent and we have never waited long for in house service. Lynn Burton Lab Associate Animal Disease Lab Galesburg, Il -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp Sent: Friday, October 26, 2012 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako vs Ventana IHC systems Does anyone have info about Dako vs Ventana IHC systems as to consistency and reliability as far as turn around time on tech repair on site.? Thanks. Gloria Tharp, BA, HTL(ASCP) Director of Operations PCA Southeast gtharp@pcasoutheast.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbarron <@t> stanford.edu Wed Nov 7 13:43:30 2012 From: fbarron <@t> stanford.edu (Frances Elizabeth Barron) Date: Wed Nov 7 13:43:33 2012 Subject: [Histonet] RE: recommended ICC protocol for use of Conjugated mouse antibodies with unconjugated mouse antibodies In-Reply-To: <2063426765.8687790.1352317054044.JavaMail.root@stanford.edu> Message-ID: <2118938242.8696725.1352317410385.JavaMail.root@stanford.edu> Hi All, Hope this day finds you well. I have two antibodies in the lab that I would like to use together for ICC. One is a mouse anti-human pre-conjugated antibody, the other is a mouse anti-human unconjugated antibody. My question is....how to get these guys to play nice with one another or should I not be worrying about it? My thought is that I have to do this sequentially, (stain first with the unconjugated, stain with the secondary, then stain with the pre-conjugated antibody?). However, I'm questioning whether or not this is necessary. Would the mouse secondary react to a conjugated antibody? Does the process of conjugating hide the secondary epitope? I have had bad luck with loosing substantial signal from the first round of primary/secondary antibody staining in a sequential staining, so I'm loathe to do it (appreciate any advice on that issue if you have it!). I can find one of the antibodies raised in another animal, but it isn't validated in human or ICC (just predicted to work). Just trying to see if I can use what I have before I go out and buy another antibody that may or may not work. Appreciate any help with this! Best, ~Francie ******************************************************* Francie Barron, Ph.D. Postdoctoral Fellow, Joseph Wu Lab Stanford University School of Medicine Lorry I. Lokey Stem Cell Research Building 265 Campus Drive, Room G1105 Stanford, CA 94305-5454 Phone: (650) 724-5564 or (650) 724-9240 Fax: (650) 723-6272 ******************************************************* From marktarango <@t> gmail.com Wed Nov 7 14:00:19 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Nov 7 14:00:23 2012 Subject: [Histonet] RE: recommended ICC protocol for use of Conjugated mouse antibodies with unconjugated mouse antibodies In-Reply-To: <2118938242.8696725.1352317410385.JavaMail.root@stanford.edu> References: <2063426765.8687790.1352317054044.JavaMail.root@stanford.edu> <2118938242.8696725.1352317410385.JavaMail.root@stanford.edu> Message-ID: Francie, Conjugated antibodes CAN be detected by secondary antibodies. So the conjugation doesn't really hide the primary antibody to the secondary. I've done it and seen it with biotin and FITC labeled antibodies. Something to consider.. Mark On Wed, Nov 7, 2012 at 11:43 AM, Frances Elizabeth Barron < fbarron@stanford.edu> wrote: > Hi All, > > Hope this day finds you well. > > I have two antibodies in the lab that I would like to use together for > ICC. One is a mouse anti-human pre-conjugated antibody, the other is a > mouse anti-human unconjugated antibody. My question is....how to get these > guys to play nice with one another or should I not be worrying about it? > > My thought is that I have to do this sequentially, (stain first with the > unconjugated, stain with the secondary, then stain with the pre-conjugated > antibody?). However, I'm questioning whether or not this is necessary. > Would the mouse secondary react to a conjugated antibody? Does the process > of conjugating hide the secondary epitope? > > I have had bad luck with loosing substantial signal from the first round > of primary/secondary antibody staining in a sequential staining, so I'm > loathe to do it (appreciate any advice on that issue if you have it!). I > can find one of the antibodies raised in another animal, but it isn't > validated in human or ICC (just predicted to work). Just trying to see if I > can use what I have before I go out and buy another antibody that may or > may not work. > > Appreciate any help with this! > > Best, > ~Francie > > ******************************************************* > > Francie Barron, Ph.D. > Postdoctoral Fellow, Joseph Wu Lab > > Stanford University School of Medicine > Lorry I. Lokey Stem Cell Research Building > 265 Campus Drive, Room G1105 > Stanford, CA 94305-5454 > > Phone: (650) 724-5564 or (650) 724-9240 > Fax: (650) 723-6272 > > ******************************************************* > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From turkekul <@t> gmail.com Wed Nov 7 14:23:21 2012 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Wed Nov 7 14:23:25 2012 Subject: [Histonet] elmer' s glue slide coating protocol Message-ID: Dear Histo Wisdom, I would like to use Elmer' s glue (I have Elmer's glue Glue-All multi-purpose glue) to coat slides so that my FFPE mouse femur sections remain on the slide after HIER during IHC. Is one type of Elmer' s glue better than other types? Do you have a working protocol? Please help me! Regards, Mesru From Caroline.Pratt <@t> uphs.upenn.edu Wed Nov 7 14:58:18 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Nov 7 14:58:26 2012 Subject: [Histonet] Devasting news on 88305TC component--Where the $18came from In-Reply-To: References: Message-ID: Anyone who is willing to share how they calculate their cost per slide, can you please email it to Caroline.pratt@uphs.upenn.edu? Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Tuesday, November 06, 2012 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Devasting news on 88305TC component--Where the $18came from Sent: Tuesday, November 06, 2012 12:35 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: RE: [Histonet] Devasting news on 88305TC component--Where the $18 came from The $18.00 figure came from an article published in Archives of Pathology and Lab Medicine: Pathology Economic Model Tool A Novel Approach to Workflow and Budget Cost Analysis in an Anatomic Pathology Laboratory David Muirhead, BSc; Patricia Aoun, MD, MPH; Michael Powell, MS, FASHP; Flemming Juncker, MBA; Jens Mollerup, MSc, PhD (Arch Pathol Lab Med. 2010;134:1164-1169) Thanks to Joe Plandowski of IOL for providing me with the reference. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 3:01 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component I wish there was a way to put a positive spin on this but I can't think of any. We can only hope it kills off client billing somehow. Whomever the "stakeholder" was that told CMS a typical 88305 costs 18 bucks, I'd love to know how he/she came up with that number. It's insultingly low. http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20p roposed%20rule%208-30-11_3.pdf I believe whoever it was had the goal to stop the proliferation of POLs. Wouldn't surprise me if they worked for a large national lab that had lost a lot of business to POLs. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rjbuesa <@t> yahoo.com Wed Nov 7 15:34:16 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 7 15:34:21 2012 Subject: [Histonet] elmer' s glue slide coating protocol In-Reply-To: References: Message-ID: <1352324056.35105.YahooMailNeo@web163105.mail.bf1.yahoo.com> Prepare a 2% solution. Immerse the slides and place them vertically so they drain the excess. To the oven at 60?C for 15 minutes. Store or use. Ren? J. ________________________________ From: mesruh turkekul To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 7, 2012 3:23 PM Subject: [Histonet] elmer' s glue slide coating protocol Dear Histo Wisdom, I would like to use Elmer' s glue (I have Elmer's glue Glue-All multi-purpose glue) to coat slides so that my FFPE mouse femur sections remain on the slide after HIER during IHC. Is one type of Elmer' s glue better than other types? Do you have a working protocol? Please help me! Regards, Mesru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DAndreas.Williams <@t> pbrc.edu Wed Nov 7 16:39:09 2012 From: DAndreas.Williams <@t> pbrc.edu (D'Andreas Williams) Date: Wed Nov 7 16:39:12 2012 Subject: [Histonet] RE: Protocol for mouse tissue on VIP Processor In-Reply-To: <3F0184241070BC41AD8C8EC2C45B50243A990B@pbrcas31.pbrc.edu> References: <3F0184241070BC41AD8C8EC2C45B50243A990B@pbrcas31.pbrc.edu> Message-ID: <3F0184241070BC41AD8C8EC2C45B50243A990C@pbrcas31.pbrc.edu> To All histology experts: We are currently using a VIP 6 Processor. We have a high demand for mouse tissue. Does anyone out there have a protocol for mouse brain (3mm sections), liver, kidney (whole or half), heart, lung, and fat? If you could share your protocol as well as vac and pressure settings we would be grateful. With Kindest Regards, D'Andreas Williams Pennington Biomedical Research Center From liz <@t> premierlab.com Wed Nov 7 16:45:57 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Nov 7 16:46:05 2012 Subject: [Histonet] RE: Protocol for mouse tissue on VIP Processor In-Reply-To: <3F0184241070BC41AD8C8EC2C45B50243A990C@pbrcas31.pbrc.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE01640B28DBAB@SBS2K8.premierlab.local> We use that processor for soft tissue we use 20 minutes per station for kidney and lung and liver - trimmed sections, whole or half kidneys will require more time anywhere from 30 to 45 minutes per station. For Brain 3 mm section we use 30 minutes per station and for fat I would go 45 minutes to 1 hour per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Andreas Williams Sent: Wednesday, November 07, 2012 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Protocol for mouse tissue on VIP Processor To All histology experts: We are currently using a VIP 6 Processor. We have a high demand for mouse tissue. Does anyone out there have a protocol for mouse brain (3mm sections), liver, kidney (whole or half), heart, lung, and fat? If you could share your protocol as well as vac and pressure settings we would be grateful. With Kindest Regards, D'Andreas Williams Pennington Biomedical Research Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Wed Nov 7 17:19:53 2012 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Nov 7 17:22:29 2012 Subject: [Histonet] overfixation with formalin In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A7F49AC7D3@EXC-MBX3.cfs.le.ac.uk> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <7722595275A4DD4FA225B92CDBF174A101A7F49AC7D3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <509B7938.411C.0039.0@health.qld.gov.au> Hello I agree. I recently saw presentation at a conference where a lab received specimens from Kiribati located in a small group of remote islands in the pacific. They receive specimens in all sorts of solutions including sea water. He was able to demonstrate good staining in specimens that had been in conc. 37% formaldehyde (no buffer) for 3 months simply by modifying antigen retrieval. Of the 55 antibodies they stain in their lab there were only 2-3 where staining was not achieved. But if they were underfixed!!! regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Queensland Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Edwards, Richard E." 11/8/2012 1:38 am >>> I have seen far more damaged/unusable (apart from H@E) tissues through under-fixation than over-fixation..................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 03 November 2012 18:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Dingersoll <@t> aplaboratories.com Wed Nov 7 19:05:06 2012 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Wed Nov 7 19:05:13 2012 Subject: [Histonet] used lab equipment Message-ID: <20121107180506.073ecbdb5144cf8a05e574ee22bfb11a.cf9456c266.wbe@email17.secureserver.net> I posted last week and got several responses. Anyone interested i obtaining this equipment, please email me what you are willing to pay< packaging, handli will accept e We have the following equipment: Leica TP1050 (Tissue Processor) Dako Autostaine RMC Products MR3 Autom Fisher Scientific IsoTemp oven 2 Ventanna Benchmarks I just took over as lab manager a couple =f months ago and I'm not< BR>sure if all of the above are operational. I do kn=w that the Benchm Price is VERY negoti Donna S. Ingersoll, B.S., HTL, CT(ASCP) Laboratory Manager< From sprice2003 <@t> gmail.com Wed Nov 7 19:46:05 2012 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Nov 7 19:46:08 2012 Subject: [Histonet] Collagen fragmentation in skin sections after heat retrieval Message-ID: Our lab has been experiencing a problem recently and we could use some input on what might be causing it and what we might be able to do to prevent it. The issue is a very noticeable fragmentation of the dermal collagen within most skin specimens, which isn't visible after H&E staining, but is after heat-retrieval. We think our retrieval is pretty gentle: It's performed in a veggie steamer (approx. 92oC) for 40 min, followed by 15 min colloing on the countertop before rinsing. We've tried a variety of charged slides to see if the collagen holds on better, but it doesn't. We think our processing is pretty routine as well. If it will help, I can send a photo of this phenomenon to anyone who's interested. Thanks, Sally From liz <@t> premierlab.com Wed Nov 7 20:06:39 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Nov 7 20:07:18 2012 Subject: [Histonet] Collagen fragmentation in skin sections after heat retrieval In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE01640B29BFF3@SBS2K8.premierlab.local> For skin and friable samples we retreive at 70C for 2 hours, works for most antibodies, but not all. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price [sprice2003@gmail.com] Sent: Wednesday, November 07, 2012 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Collagen fragmentation in skin sections after heat retrieval Our lab has been experiencing a problem recently and we could use some input on what might be causing it and what we might be able to do to prevent it. The issue is a very noticeable fragmentation of the dermal collagen within most skin specimens, which isn't visible after H&E staining, but is after heat-retrieval. We think our retrieval is pretty gentle: It's performed in a veggie steamer (approx. 92oC) for 40 min, followed by 15 min colloing on the countertop before rinsing. We've tried a variety of charged slides to see if the collagen holds on better, but it doesn't. We think our processing is pretty routine as well. If it will help, I can send a photo of this phenomenon to anyone who's interested. Thanks, Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Wed Nov 7 20:52:51 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Wed Nov 7 20:52:55 2012 Subject: [Histonet] Re: Histonet Digest, Vol 108, Issue 9 In-Reply-To: <509a80ce.23183c0a.25a3.ffff91feSMTPIN_ADDED@mx.google.com> References: <509a80ce.23183c0a.25a3.ffff91feSMTPIN_ADDED@mx.google.com> Message-ID: > Message: 4 > Date: Tue, 6 Nov 2012 11:41:26 -0800 (PST) > From: Amy Lee > Subject: [Histonet] How to fix frozen section? > To: histonet > Message-ID: > <1352230886.68993.YahooMailClassic@web126002.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello, > > I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? > > > Thanks, > > Amy > Amy, Here's my method (based only on personal experiences); 1st) Remove frozen tissue section/s from freezer 2nd) Immediatly pre-fix in 10% NFB @ RT for 10 min (do NOT thawed slides @ RT) "I found some protein leak out if pre-fix with Acetone without 10%NFB" 3rd) Re-fix in Acetone for addition 5min - 10min @ -20c 4th) Air dry slides with fan for ~ 5 min (or RT for 10 min). "I purchase $10 portable fan from drug store" 5th) Wash off any excess OCT with washing buffer 3x 6th) Proceed your IHC staining...... Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology madeleine_h@elcaminohospital.org From gu.lang <@t> gmx.at Thu Nov 8 00:52:39 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 8 00:52:49 2012 Subject: AW: [Histonet] overfixation with formalin In-Reply-To: <509B7938.411C.0039.0@health.qld.gov.au> References: <013701cdb9f2$ca30c370$5e924a50$@gmx.at> <7722595275A4DD4FA225B92CDBF174A101A7F49AC7D3@EXC-MBX3.cfs.le.ac.uk> <509B7938.411C.0039.0@health.qld.gov.au> Message-ID: <001201cdbd7d$a6074b70$f215e250$@gmx.at> Thank you Tony for this interesting ?large-trial? on tissue-fixation. And this also supports my opinion about formalin-concetrations. The necessary modification of AR is certainly a point, why interlab-standardisation is a good point. Regards Gudrun Von: Tony Reilly [mailto:Tony_Reilly@health.qld.gov.au] Gesendet: Donnerstag, 08. November 2012 00:20 An: 'gu.lang@gmx.at'; Richard E. Edwards; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] overfixation with formalin Hello I agree. I recently saw presentation at a conference where a lab received specimens from Kiribati located in a small group of remote islands in the pacific. They receive specimens in all sorts of solutions including sea water. He was able to demonstrate good staining in specimens that had been in conc. 37% formaldehyde (no buffer) for 3 months simply by modifying antigen retrieval. Of the 55 antibodies they stain in their lab there were only 2-3 where staining was not achieved. But if they were underfixed!!! regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Queensland Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Edwards, Richard E." 11/8/2012 1:38 am >>> I have seen far more damaged/unusable (apart from H@E) tissues through under-fixation than over-fixation..................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 03 November 2012 18:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overfixation with formalin Hi histonetters! I'm just attending a histo-course, where the teacher told us his opinion about overfixation. For him overfixation takes place in any formaldehyde solution with a concentration above 5%. This should cause the margin-artefact, that leads to false-positive IHC at the margins of the tissue and to false-negative results in the center. The higher concetrated fixative should harden and shrink the surface, so it cant be penetrated any more by the fixative. I told him about the publication of Cecil Fox, who saw shrinkage only in solutions with formaldehyde concentration above 30% (I think) and said, that the methanol-part is responsible for that. I believe, that these margin-artefacts are due to drying at the time of biopsy or an effect of the needle-shot itself. (But believing is no evidence) In our lab we use 8% formaldehyde as standard fixative, buffered with low-molar phosphatebuffer. There are no complains from the doctors about margins. Please help me with the histonet-wisdom. What's your opinion? Bye Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From mjdessoye <@t> commonwealthhealth.net Thu Nov 8 06:20:25 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Thu Nov 8 06:20:34 2012 Subject: [Histonet] Charges for cutting research case In-Reply-To: Message-ID: We have worked out a cost for cutting and staining H&E's, so we basically take that cost and mark it up. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, November 07, 2012 12:44 PM To: histonet-bounces@lists.utsouthwestern.edu Cc: histonet Subject: [Histonet] Charges for cutting research case I would like some direction in regards to how to charge a client for cutting multiple slides and levels anywhere between 10 to 50 slides per block of a Technical component case. These would be research cases. Any help directing me would be greatly appreciated. Thank, Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From MSHERWOOD <@t> PARTNERS.ORG Thu Nov 8 09:00:50 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Nov 8 09:01:44 2012 Subject: [Histonet] Re: [Histology] Congo Red Stain Message-ID: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> To all: Would people please share their method for doing Congo Red staining? (We are not interested in making up the reagents from powder form). Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From portera <@t> msu.edu Thu Nov 8 09:11:30 2012 From: portera <@t> msu.edu (Amy Porter) Date: Thu Nov 8 09:11:39 2012 Subject: [Histonet] Re: [Histology] Congo Red Stain In-Reply-To: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> References: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> Message-ID: <000601cdbdc3$54cb3b90$fe61b2b0$@edu> Sigma Aldrich makes a very nice kit for this. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, November 08, 2012 10:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: [Histology] Congo Red Stain To all: Would people please share their method for doing Congo Red staining? (We are not interested in making up the reagents from powder form). Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Thu Nov 8 09:26:40 2012 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Thu Nov 8 09:30:02 2012 Subject: [Histonet] RE: Re: [Histology] Congo Red Stain In-Reply-To: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> References: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> Message-ID: <12ECD7346266D74691EC2BFC75285E450114691B@BFL323E10.pathmdlabs.local> I've tried kits from both Richard Allan/Thermo and American Master Tech. Both staining methods are similar and very easy to perform - 20 minutes in Congo Red and counterstain in Hematoxylin. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, November 08, 2012 7:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: [Histology] Congo Red Stain To all: Would people please share their method for doing Congo Red staining? (We are not interested in making up the reagents from powder form). Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmackinnon <@t> dermatologyconsultants.com Thu Nov 8 09:59:50 2012 From: mmackinnon <@t> dermatologyconsultants.com (Michelle M. Mackinnon) Date: Thu Nov 8 09:59:09 2012 Subject: [Histonet] Manual IHC for Melan-A Message-ID: <7141C086431ED24FB7F1E0E66028BC1B0404494A@mail.derm.local> Has anyone done manual IHC for Melan-A? If yes, who help set up the manual protocol? Thanks, Michelle Michelle M MacKinnon HT, (ASCP) Histology Supervisor Dermatology Consultants. PA 1215 Town Centre Drive, Suite 200 Eagan, MN 55123 Phone: 651-255-3462 Fax: 651-255-3461 From gu.lang <@t> gmx.at Thu Nov 8 10:32:50 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 8 10:34:08 2012 Subject: AW: [Histonet] Re: [Histology] Congo Red Stain In-Reply-To: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> References: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> Message-ID: <000901cdbdce$bb67d830$32378890$@gmx.at> We use the Merck kit but have turned the order of reagenses: first congored and differentiation, then hemalaun. That masks much of unwanted staining. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sherwood, Margaret Gesendet: Donnerstag, 08. November 2012 16:01 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Re: [Histology] Congo Red Stain To all: Would people please share their method for doing Congo Red staining? (We are not interested in making up the reagents from powder form). Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Nov 8 10:44:21 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Nov 8 10:44:38 2012 Subject: [Histonet] RE: Manual IHC for Melan-A In-Reply-To: <7141C086431ED24FB7F1E0E66028BC1B0404494A@mail.derm.local> References: <7141C086431ED24FB7F1E0E66028BC1B0404494A@mail.derm.local> Message-ID: We do manual staining for everything - here is the abbreviated version for Melan A - Thermo Scientific MS799 Clone A103; Mouse MAb Isotype IgG1 Protocol: 1. Dewax and hydrate sections 2. Retrieve with HIER - EDTA pH 8 20 min 3. Block with NGS for 15 min 4. Treat with primary Ab for 30 min 5. Apply Link #1 - biotinylated ?-mouse 6. Apply Link #2 - streptavidin enzyme complex 7. Rinse with water and apply chromogen 8. Rinse with dH2O 9. Counterstain, dry and coverslip Results: Staining is cytoplasmic Granular cytoplasmic staining of adrenal cortical cells; minimal or no staining of kidney Equivalent results using canine and feline tissue during validation Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle M. Mackinnon Sent: Thursday, November 08, 2012 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual IHC for Melan-A Has anyone done manual IHC for Melan-A? If yes, who help set up the manual protocol? Thanks, Michelle Michelle M MacKinnon HT, (ASCP) Histology Supervisor Dermatology Consultants. PA 1215 Town Centre Drive, Suite 200 Eagan, MN 55123 Phone: 651-255-3462 Fax: 651-255-3461 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Nov 8 11:04:43 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 8 11:04:50 2012 Subject: [Histonet] Manual IHC for Melan-A In-Reply-To: <7141C086431ED24FB7F1E0E66028BC1B0404494A@mail.derm.local> References: <7141C086431ED24FB7F1E0E66028BC1B0404494A@mail.derm.local> Message-ID: <1352394283.50549.YahooMailNeo@web163106.mail.bf1.yahoo.com> If you use an autostainer, just repeat all the steps and incubation times, but manually. Ren? J. ________________________________ From: Michelle M. Mackinnon To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, November 8, 2012 10:59 AM Subject: [Histonet] Manual IHC for Melan-A Has anyone done manual IHC for Melan-A?? If yes, who help set up the manual protocol? Thanks, Michelle Michelle M MacKinnon HT, (ASCP) Histology Supervisor Dermatology Consultants. PA 1215 Town Centre Drive, Suite 200 Eagan, MN 55123 Phone: 651-255-3462 Fax: 651-255-3461 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Nov 8 11:06:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 8 11:06:58 2012 Subject: AW: [Histonet] Re: [Histology] Congo Red Stain In-Reply-To: <000901cdbdce$bb67d830$32378890$@gmx.at> References: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> <000901cdbdce$bb67d830$32378890$@gmx.at> Message-ID: <1352394411.50979.YahooMailNeo@web163106.mail.bf1.yahoo.com> For a strong staining with Congo Red and nuclear staining with hematoxylin, do the hematoxylin first of all steps and later stain for Congo Red. Doing the hematoxylin last will produce weak nuclear staining. Ren? J. ________________________________ From: Gudrun Lang To: "'Sherwood, Margaret'" Cc: Histonet@lists.utsouthwestern.edu Sent: Thursday, November 8, 2012 11:32 AM Subject: AW: [Histonet] Re: [Histology] Congo Red Stain We use the Merck kit but have turned the order of reagenses: first congored and differentiation, then hemalaun. That masks much of unwanted staining. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sherwood, Margaret Gesendet: Donnerstag, 08. November 2012 16:01 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Re: [Histology] Congo Red Stain To all: Would people please share their method for doing Congo Red staining?? (We are not interested in making up the reagents from powder form). Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Thu Nov 8 12:28:27 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Nov 8 12:28:34 2012 Subject: [Histonet] RE: Re: [Histology] Congo Red Stain In-Reply-To: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> References: <16F356143B1CE2459BC129BF68AD0F0F03F2B390@PHSX10MB25.partners.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF568A2D@EXCHANGEMB1.hmc.hurleymc.com> We used to use the traditional Congo Red for Amyloid, but our docs were not happy with the color whether purchased from a kit or manually made. We searched and tried "Amyloid Red" from Anatech, and have been doing it ever since. It is a brighter red color. Nothin' like a happy doc! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sherwood, Margaret [MSHERWOOD@PARTNERS.ORG] Sent: Thursday, November 08, 2012 10:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: [Histology] Congo Red Stain To all: Would people please share their method for doing Congo Red staining? (We are not interested in making up the reagents from powder form). Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Nov 8 12:51:10 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Nov 8 12:51:29 2012 Subject: [Histonet] RELIA Histology Careers Bulletin 11-8-2012 A mid-week update. Message-ID: <155d01cdbde2$055b23d0$10116b70$@earthlink.net> Hi Histonetters! How are you? I wanted to drop you a quick line mid-week and let you know about some of the positions I am working on. I have a number of really great opportunities For ASCP certified or eligible histologists. All of these positions are full time permanent and offer excellent pay, great benefits and in some cases bonuses. The great thing about these opportunities is that if you are ready to move right away they are too and if you want to pursue a position with a start date after the holidays that works as well !! RELIA Histology Spotlight Opportunity: Histotechnician/Histotechnologist for a growing lab in Augusta, GA. This is a full time permanent position early morning shift M-F. They offer outstanding benefits a great group of people to work with and opportunity for advancement! Here is a list of some of our other current openings that I am excited about! * Augusta, GA - Histotechnician/Histotechnologist HT/HTL required * Harrisonburg, VA - Histotechnician Full time days HT required * Charlotte, NC - Growing Hospital based lab All shifts. HT/HTL req. * Nashville, TN - Private Path Lab Afternoon shift ASCP HT/HTL or eligible * Collegeville, PA New Lab - Day shift HT/HTL and CLIA qual to gross * Reading, PA - In Office Path Lab - Dermpath Histotech Day Shift HT/HTL and CLIA qual to gross * Dallas, TX - Private Reference Lab - IHC Tech ASCP HT/HTL, QIHC preferred. * Portland, ME - Private Reference Lab - Histotech ASCP HT/HTL or eligible * Topeka, KS - Lead Histotech/Supervisor ASCP HT/HTL * Long Island, NY - Electron Microscopy Lead Tech/Tech - NYS lic or elig. If you are interested in participating in my referral program in addition to histotechs for these positions I also need cytotechs in TN and SD. If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tkngflght <@t> yahoo.com Thu Nov 8 16:23:16 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Nov 8 16:23:20 2012 Subject: [Histonet] Gonadotrophin releasing hormone Message-ID: <1352413396.94328.YahooMailNeo@web39403.mail.mud.yahoo.com> Hi everyone- ? Would you help me find a new vendor for this antibody: ? Gonadotrophin-Releasing Hormone Receptor, Moust monoclonal. ? It was vended by Vector Labs out of Burlingame CA?as VP-G811.? ? We're in the MIDDLE of a research?study and having to change antibodies is going to be tough! ? The original vendor said it was discontinues with no further information.? Usually?vendors can tell us why and I can't dig up anything more on this--hoping maybe they lost?a contract or something where we can find it elsewhere...any help is?VERY much appreciated.?? ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From hlukey <@t> msn.com Thu Nov 8 16:33:08 2012 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Nov 8 16:33:11 2012 Subject: [Histonet] BioInventory (Pathology) software recommendations? Message-ID: Hi all, Can I ask for recommendations for a Tissue block repository software? It needs to incorporate FFPE blocks and their usage in research projects like TMA construction or RNA/DNA testing. We will be most impressed with ease of use and pre-built categories with barcoding. It must be able to incorporate ~15 million entries. Communication with CoPath/Cerner is a plus. We have tried various companies, and are currently using one of them, but the software was not designed to capture workload and is a chore to use. I would appreciate any advice. Vendors welcome. Thank you in advance, Hugh Hawaii From rsrichmond <@t> gmail.com Thu Nov 8 16:34:03 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Nov 8 16:34:08 2012 Subject: [Histonet] Re: Congo Red Stain Message-ID: If you're new to the congo red stain for amyloid: You must have a positive control. Most of the controls I've seen recently have been medullary carcinomas of the thyroid, which usually (though not always) contain stainable amyloid. I've asked many times on Histonet and never got a reply: amyloidosis is fairly easily produced in experimental animals. Why isn't this material used by pathologists as an amyloid control? Supposedly paraffin sections of control tissue must be cut within a month of use - the undeparaffinized sections deteriorate, though the paraffin block themselves do not. You must examine controls and patient sections using polarization. If you don't have a polarizer on your microscope - and most pathologists don't - then don't do the stain. The standard "jiss" - jiss use a pair of broken sunglasses - is not satisfactory. Anatech offers a stain they call "Amyloid Red" (it's clearly characterized in their promotional material) - has anyone used it? Bob Richmond Samurai Pathologist Maryville TN From allison-malandra <@t> uiowa.edu Thu Nov 8 16:52:42 2012 From: allison-malandra <@t> uiowa.edu (Malandra, Allison E) Date: Thu Nov 8 16:52:46 2012 Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic Message-ID: <451035461C585B4BAEBFC8ADAC7F804A23FBBE09@HC-MAILBOXC1-N5.healthcare.uiowa.edu> Hi there - We are trying to stain osteoporotic bone that was embedded using Spurr plastic. We need to be able to differentiate osteoid from mineralized bone. We have tried Gio's trichrome and Goldner's trichrome with no success, both surface staining and on deplasticized slides. We are thinking of trying Von Kossa, does anyone have a recipe/protocol for this? Or other types of stains that you have had success with? Thank you! Allison Malandra, DVM University of Iowa, Bone Healing Research Lab ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From ratliffjack <@t> hotmail.com Thu Nov 8 17:51:49 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Nov 8 17:51:55 2012 Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic In-Reply-To: <451035461C585B4BAEBFC8ADAC7F804A23FBBE09@HC-MAILBOXC1-N5.healthcare.uiowa.edu> References: <451035461C585B4BAEBFC8ADAC7F804A23FBBE09@HC-MAILBOXC1-N5.healthcare.uiowa.edu> Message-ID: Allison, I am not exactly certain if this protocol will work for Spurr's embedded specimens, but here is the protocol that I use for deplasticized sections of methyl methacrylate embedded undemineralized bone (non-decalcified): Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval 100% EtOH - room temp for 5 min 95% EtOH - room temp for 5 min 70% EtOH - room temp for 5 min DI H2O - room temp for 5 min Silver Nitrate (20 g) + DI H2O (400 ml) - room temp (in dark protected from light) for 5 min DI H2O rinse - room temp (protected from light) for 1 min DI H2O rinse - room temp (protected from light) for 1 min DI H2O rinse - room temp (protected from light) for 1 min Sodium Carbonate (22.5 G) + DI H2O (337.5 ml) + 37% Formaldehyde (112.5 ml) - room temp (protected from light) for 2 min DI H2O rinse - room temp for 1 min DI H2O rinse - room temp for 1 min Sodium Thiosulfate (40 g) + Potassium Ferricyanide (2 g) + DI H2O (420 ml) Solution - room temp for 30 sec(solution stable for 30-60 min after addition of potassium ferricyanide) Running Tap Water Rinse - 10 min Counterstain w/ MacNeal's Tetrachrome (12 g) + DI H2O (600 ml) - room temp for 6-8 min(stir continuously w/ heat @ 60C for 48 hours covered, then filter with paper towel) DI H2O rinse - room temp for 1 min DI H2O rinse - room temp for 1 min DI H2O rinse - room temp for 1 min 70% EtOH - room temp for 1 min 100% EtOH - room temp for 1 min Xylenes - room temp for 3 min Xylenes - room temp for 3 min Coverslip w/ Eukitt Hope this helps! Please feel free to contact me if you have any questions or concerns. Best Regards, Jack Jack L RatliffOwner, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology (317) 281-1975jratliff@ratliffhistology.com LinkedIn Profile: http://www.linkedin.com/profile/edit?trk=hb_tab_pro_top > From: allison-malandra@uiowa.edu> To: histonet@lists.utsouthwestern.edu > Date: Thu, 8 Nov 2012 22:52:42 +0000 > Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic > > Hi there - > > We are trying to stain osteoporotic bone that was embedded using Spurr plastic. We need to be able to differentiate osteoid from mineralized bone. We have tried Gio's trichrome and Goldner's trichrome with no success, both surface staining and on deplasticized slides. We are thinking of trying Von Kossa, does anyone have a recipe/protocol for this? Or other types of stains that you have had success with? Thank you! > > Allison Malandra, DVM > University of Iowa, Bone Healing Research Lab > > > ________________________________ > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chak_bou <@t> yahoo.com Thu Nov 8 19:02:03 2012 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Thu Nov 8 19:02:06 2012 Subject: [Histonet] SOX-10 Message-ID: <1352422923.68597.YahooMailClassic@web160804.mail.bf1.yahoo.com> Hi Histonet, Does anyone using SOX-10 antibody? if so can you share with me the protocol? dilution? Thank you so much. Chakib Boussahmain HTL(ASCP) From Lynn.O'Donnell <@t> wchn.org Fri Nov 9 07:25:01 2012 From: Lynn.O'Donnell <@t> wchn.org (O'Donnell, Lynn M.) Date: Fri Nov 9 07:25:15 2012 Subject: [Histonet] Thermo Stainmate Max In-Reply-To: <1352394283.50549.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <7141C086431ED24FB7F1E0E66028BC1B0404494A@mail.derm.local> <1352394283.50549.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: Hi, Have any of you used the Thermo Stainmate Mix? It has 10 dishes holding 200ml of fluid? Am looking for opinions. We are considering getting this for our rush FNAs. Thanks. ______________________________ Lynn O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-7846 lynn.o'donnell@wchn.org From ngomez <@t> neiker.net Fri Nov 9 07:55:23 2012 From: ngomez <@t> neiker.net (Nieves Gomez) Date: Fri Nov 9 07:55:44 2012 Subject: [Histonet] formalin substitutes. HELP Message-ID: <653C9D5D1FE28B4592775182B08B4B2DE6A835@nkderposta1.intranat.wan> Dear Histonetters, I'm new in the net. I work as Pathologist in a Vet Lab in Spain. Because formalin is toxic our Lab is for the practice of using alternative fixatives. I think the main viable alternative is glyoxal based formulas, but I have so many questions that Commercials don't know or don't want to answer me. For example, have a MSDS or is it accessible? Really is less hazardous than formalin or just is not checked? (the advantages and desadvantages of formalin are known for at least 100 years). Related to this, I think the glyoxal is suggested as a formalin substitute in an article in 1940's and now it is sold as a "new product" and most of the products are sold as "green", "no-toxic" or "non harmful". In my opinion, a fixative can not be "non-toxic" if you want it fixed tissues. Another question is the time needed to fix tissues or the ratio volume specimen/fixative. To the first point, I have read an article that mentions there is mould growth in specimens over time. Are we changing a "chemical risk" to a "biological risk"? In my lab we have a specifically workstation for the gross examination and sectioning of specimens, and we wear all the Personal Protective Equipment needed (formalin chemical filters, gloves, googles...) that minimizes the risk (the chemical risk not the biological risk). It is believed that formalin given time will kill any microorganisms (or spores) present in tissues, mycobacteria also.. what about these new products? Are they germicidal? I do not get to appreciate the morfological changes (nuclear changes, lysis of erithrocites, eosinophilia...) because they are well documented. My aim is to know if there is any Lab that works with any formalin substitute routinely to aks these questions. Please, help me. Thanks and have a nice weekend Nieves Gomez Veterinary Pathologist Animal Health Department NEIKER-Tecnalia Berreaga, 1. 48160 Derio. Bizkaia. Spain. ngomez@neiker.net From brett_connolly <@t> merck.com Fri Nov 9 08:16:32 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Nov 9 08:16:39 2012 Subject: [Histonet] RE: formalin substitutes. HELP In-Reply-To: <653C9D5D1FE28B4592775182B08B4B2DE6A835@nkderposta1.intranat.wan> References: <653C9D5D1FE28B4592775182B08B4B2DE6A835@nkderposta1.intranat.wan> Message-ID: Hello Nieves, Here in the US Anatech LTD sells a gyloxal fixative called Prefer. I have attached a link to the website. Go to the MSDS menu and click on 'Prefer' to pull up the MSDS. http://www.anatechltdusa.com/ Below is an old post from the HistoNet that might be helpful. I suggest you contact Ada Feldman at Anatech, as she should be able to address your issues Regards, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 As the developers of the Prefer fixative we would like to address some of the issues. Interchangeability of glyoxal products: The manufacturer of each fixative should be able to provide the information necessary to work with their fixative in conjunction with any other fixative that a lab may be using. Just as an example, Hollandes is not compatible with NBF and requires special attention. As for Prefer we can say it is compatible with the majority of other fixatives, glyoxal or not. Limited time in the fixative: There is a slight reduction in staining intensity after several weeks, but increasing staining time corrects this. So tissues are not rendered unstainable. Transition period: This is true any time you are switching fixatives or processing methods. Eosinophila: Your statements here are true. Lysis of erythrocytes: True again. It is often seen as an advantage because diagnostics cells are easier to see. Breast cancer: It would seem that the improved nuclear morphology would make marked nuclear variation easier to determine. Prostate biopsies: Hope you can get a response from any of the glyoxal users with prostate biopsies. We would be interested in this answer also. Ada T. Feldman, MS, HT/HTL(ASCP) ANATECH LTD. 1020 Harts Lake Road Battle Creek, MI 49015 Phone: 800.262.8324 Fax: 269.964.8084 email: adafeldman@anatechltdusa.com website: www.anatechltdusa.com On Jul 14, 2006, at 4:13 PM, RSRICHMOND@aol.com wrote: > The people at Anatech, makers of Prefer fixative, have published a > review of > glyoxal fixation that every pathologist and histotechnologist ought > to read. > This working surgical pathologist would like to add - and solicit - > some > comments on Histonet. > > "Glyoxal Fixation and Its Relationship to Immunohistochemistry". > Richard W. > Dapson, Ada T. Feldman, and Dee Wolfe. Anatech Limited, Battle > Creek MI. The > Journal of Histotechnology June 2006;29:65-76. > > I don't want to change, but I think we all need to be prepared for > the day > when a manager walks into our laboratory, or a letter from a > regulatory agency > arrives in the mail, telling us that we have to get rid of > formaldehyde right > now. Probably glyoxal is the only acceptable substitute, and we all > need to > have a look at it. I have a number of questions. > > Interchangeability of glyoxal products: Prefer is described as a > buffered > solution of glyoxal with a pH of about 4. The formula is a trade > secret. > Competing glyoxal products probably also have trade-secret > formulas. So can a lab > change brands of buffered glyoxal without problems, or does it have > to stay with a > particular brand with its trade-secret buffering? - We've seen a > similar > problem with distilling aliphatic xylene substitutes: every one of > them requires a > separate distillation routine, at least on a spinning-band still. - > I'll > leave it to John Kiernan to comment on the appropriateness of trade- > secret > reagents in histopathology. > > Limited time in the fixative: Tissue can be left in neutral > buffered formalin > for quite a long time and still be stainable, but tissue stored in > glyoxal > becomes unstainable after about two weeks. Can glyoxal fixed tissue be > transferred to 70% ethanol for more prolonged storage? - A very > occasional surgical > specimen requires additional blocks after a week - a bigger problem > will be the > pathologist who doesn't trim his autopsies promptly. > > Transition period: A laboratory changing to glyoxal would have to > keep IHC > procedures for both fixatives working for some time. There would > have to be some > way to identify whether a block was fixed in formaldehyde or glyoxal. > > Eosinophilia: One ought to be able to distinguish eosinophils from > neutrophils in tissue sections by nuclear morphology, without > having to see granules. > But quantitation of eosinophils - needed in an increasing number of > GI biopsy > situations - could be a problem. We might need an IHC for > eosinophils in some of > these settings. > > Lysis of erythrocytes: Not much of a problem, since we're used to > it with > acid fixatives anyway. > > Breast cancer: Elimination of nuclear bubble artifact in breast biopsy > specimens may raise the apparent nuclear grade of tumors, and thus > increase > Nottingham (Elston-Ellis) scores. > > Prostate biopsies: I'd want to see some prostate biopsies - is > somebody from > OURLab in Nashville still on this list? - with formaldehyde > fixation, nucleoli > are a strong criterion of malignancy, and if glyoxal fixation > demonstrates > nucleoli in benign ductal epithelium, this criterion is lost. > > Bob Richmond > Knoxville TN and Gastonia NC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nieves Gomez Sent: Friday, November 09, 2012 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin substitutes. HELP Dear Histonetters, I'm new in the net. I work as Pathologist in a Vet Lab in Spain. Because formalin is toxic our Lab is for the practice of using alternative fixatives. I think the main viable alternative is glyoxal based formulas, but I have so many questions that Commercials don't know or don't want to answer me. For example, have a MSDS or is it accessible? Really is less hazardous than formalin or just is not checked? (the advantages and desadvantages of formalin are known for at least 100 years). Related to this, I think the glyoxal is suggested as a formalin substitute in an article in 1940's and now it is sold as a "new product" and most of the products are sold as "green", "no-toxic" or "non harmful". In my opinion, a fixative can not be "non-toxic" if you want it fixed tissues. Another question is the time needed to fix tissues or the ratio volume specimen/fixative. To the first point, I have read an article that mentions there is mould growth in specimens over time. Are we changing a "chemical risk" to a "biological risk"? In my lab we have a specifically workstation for the gross examination and sectioning of specimens, and we wear all the Personal Protective Equipment needed (formalin chemical filters, gloves, googles...) that minimizes the risk (the chemical risk not the biological risk). It is believed that formalin given time will kill any microorganisms (or spores) present in tissues, mycobacteria also.. what about these new products? Are they germicidal? I do not get to appreciate the morfological changes (nuclear changes, lysis of erithrocites, eosinophilia...) because they are well documented. My aim is to know if there is any Lab that works with any formalin substitute routinely to aks these questions. Please, help me. Thanks and have a nice weekend Nieves Gomez Veterinary Pathologist Animal Health Department NEIKER-Tecnalia Berreaga, 1. 48160 Derio. Bizkaia. Spain. ngomez@neiker.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Fri Nov 9 08:24:58 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 9 08:25:06 2012 Subject: [Histonet] formalin substitutes. HELP In-Reply-To: <653C9D5D1FE28B4592775182B08B4B2DE6A835@nkderposta1.intranat.wan> References: <653C9D5D1FE28B4592775182B08B4B2DE6A835@nkderposta1.intranat.wan> Message-ID: <1352471098.64076.YahooMailNeo@web163106.mail.bf1.yahoo.com> Nieves: NO formalin substitute will work in the same way as formalin and the solution is not to start testing other "substitutes" that will make your life miserable and your sections and staining procedures of sub-standard quality. The solution is: 1- to use LESS amounts of formalin (a 5:1 volume is more than enough); 2- have a well ventilated area; 3- do NOT prepare your own buffered formalin; buy pre-filled sample bottles; and 4- never handle formalin more than absolutely necessary. Under separate cover I am sending you 2 articles I wrote on this subject Ren? J. ________________________________ From: Nieves Gomez To: histonet@lists.utsouthwestern.edu Sent: Friday, November 9, 2012 8:55 AM Subject: [Histonet] formalin substitutes. HELP Dear Histonetters, I'm new in the net. I work as Pathologist in a Vet Lab in Spain. Because formalin is toxic our Lab is for the practice of using alternative fixatives. I think the main viable alternative is glyoxal based formulas, but I have so many questions that Commercials don't know or don't want to answer me. For example, have a MSDS or is it accessible? Really is less hazardous than formalin or just is not checked? (the advantages and desadvantages of formalin are known for at least 100 years). Related to this, I think the glyoxal is suggested as a formalin substitute in an article in 1940's and now it is sold as a "new product" and most of the products are sold as "green", "no-toxic" or "non harmful". In my opinion, a fixative can not be "non-toxic" if you want it fixed tissues. Another question is the time needed to fix tissues or the ratio volume specimen/fixative. To the first point, I have read an article that mentions there is mould growth in specimens over time. Are we changing a "chemical risk" to a "biological risk"? In my lab we have a specifically workstation for the gross examination and sectioning of specimens, and we wear all the Personal Protective Equipment needed (formalin chemical filters, gloves, googles...) that minimizes the risk (the chemical risk not the biological risk).? It is believed that formalin given time will kill any microorganisms (or spores) present in tissues, mycobacteria also.. what about these new products? Are they germicidal? I do not get to appreciate the morfological changes (nuclear changes, lysis of erithrocites, eosinophilia...) because they are well documented. My aim is to know if there is any Lab that works with any formalin substitute routinely to aks these questions. Please, help me. Thanks and have a nice weekend Nieves Gomez Veterinary Pathologist Animal Health Department NEIKER-Tecnalia Berreaga, 1. 48160 Derio. Bizkaia. Spain. ngomez@neiker.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 9 09:28:44 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 9 09:28:45 2012 Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic In-Reply-To: References: <451035461C585B4BAEBFC8ADAC7F804A23FBBE09@HC-MAILBOXC1-N5.healthcare.uiowa.edu> Message-ID: <332D680CD16347B085ED3F211152DB37@DESKTOP3> Allison, I had a great von kossa protocol for this but it was on glycol methacrylate embedded bone, here it is, it uses the fluorescence property of eosin which will stain the osteoid with the calcified bone covered by the silver von kossa. GMA is not removed Rinse 5 micron sections in dih20, use clean glassware Make 2% silver nitrate, put on the slides and expose them to uv (a lamp or even in the window will do, for about 20 min. The calcified bone should turn black Rinse well in dih20, you can fix the silver with sodium thiosulfate but do it quickly as it does remove some silver, rinse well in dih20 Hematoxylin nuclear stain, we used Gills#3 for 5 min., wash in tap water, blue in ammonia water, wash well in tap water then to dih20 Make a 0.2% solution of aqueous eosin y, stain slides in this for 20 min., rinse in dih20 and airdry, coverslip with permount. You can measure the calcified bone and nuclei in the light microscope, then use a fluorescent scope with a fitc filter to see the osteoid, it will be green just on the surface of the calcified bone which is black from the silver. We got a total sample area measurement, calcified area as a percentage of total area, and osteoid area as a percentage of total area. We did other measurements as well such as number of osteoclasts (we used a Acid phosphotase (TRAP) enzyme histochemical stain for this) and number of osteoblast (can use alkaline phosphotase enzyme histochemical stain for these or we just counted them from the VK slide with the heme nuclear stain by morphology, they will be lining the surface of the bone, very flat and run together.) Hope this helps. Goldner TC is also used to measure osteoid but this worked best for us. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, November 08, 2012 4:52 PM To: Malandra, Allison E; Histonet Subject: RE: [Histonet] Stain for osteoporotic bone in Spurr plastic Allison, I am not exactly certain if this protocol will work for Spurr's embedded specimens, but here is the protocol that I use for deplasticized sections of methyl methacrylate embedded undemineralized bone (non-decalcified): Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval 100% EtOH - room temp for 5 min 95% EtOH - room temp for 5 min 70% EtOH - room temp for 5 min DI H2O - room temp for 5 min Silver Nitrate (20 g) + DI H2O (400 ml) - room temp (in dark protected from light) for 5 min DI H2O rinse - room temp (protected from light) for 1 min DI H2O rinse - room temp (protected from light) for 1 min DI H2O rinse - room temp (protected from light) for 1 min Sodium Carbonate (22.5 G) + DI H2O (337.5 ml) + 37% Formaldehyde (112.5 ml) - room temp (protected from light) for 2 min DI H2O rinse - room temp for 1 min DI H2O rinse - room temp for 1 min Sodium Thiosulfate (40 g) + Potassium Ferricyanide (2 g) + DI H2O (420 ml) Solution - room temp for 30 sec(solution stable for 30-60 min after addition of potassium ferricyanide) Running Tap Water Rinse - 10 min Counterstain w/ MacNeal's Tetrachrome (12 g) + DI H2O (600 ml) - room temp for 6-8 min(stir continuously w/ heat @ 60C for 48 hours covered, then filter with paper towel) DI H2O rinse - room temp for 1 min DI H2O rinse - room temp for 1 min DI H2O rinse - room temp for 1 min 70% EtOH - room temp for 1 min 100% EtOH - room temp for 1 min Xylenes - room temp for 3 min Xylenes - room temp for 3 min Coverslip w/ Eukitt Hope this helps! Please feel free to contact me if you have any questions or concerns. Best Regards, Jack Jack L RatliffOwner, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology (317) 281-1975jratliff@ratliffhistology.com LinkedIn Profile: http://www.linkedin.com/profile/edit?trk=hb_tab_pro_top > From: allison-malandra@uiowa.edu> To: histonet@lists.utsouthwestern.edu > Date: Thu, 8 Nov 2012 22:52:42 +0000 > Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic > > Hi there - > > We are trying to stain osteoporotic bone that was embedded using Spurr plastic. We need to be able to differentiate osteoid from mineralized bone. We have tried Gio's trichrome and Goldner's trichrome with no success, both surface staining and on deplasticized slides. We are thinking of trying Von Kossa, does anyone have a recipe/protocol for this? Or other types of stains that you have had success with? Thank you! > > Allison Malandra, DVM > University of Iowa, Bone Healing Research Lab > > > ________________________________ > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Nov 9 11:34:20 2012 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Nov 9 11:34:36 2012 Subject: [Histonet] decal affecting IHC Message-ID: Does decalcifying tissue (Calex II) affect the antibody reaction for IHC in bony tissue? Diana From ratliffjack <@t> hotmail.com Fri Nov 9 11:41:23 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Nov 9 11:41:31 2012 Subject: [Histonet] decal affecting IHC In-Reply-To: References: Message-ID: <2DB391C2-E776-4F92-98FC-2245C940AB32@hotmail.com> I do not know what is in Calex II but high concentration HCl can have a negative affect. We use 5% formic acid without issue. Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Nov 9, 2012, at 11:34 AM, Diana McCaig wrote: > Does decalcifying tissue (Calex II) affect the antibody reaction for IHC in bony tissue? > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Nov 9 11:47:09 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 9 11:47:13 2012 Subject: [Histonet] decal affecting IHC In-Reply-To: References: Message-ID: <1352483229.63600.YahooMailNeo@web163105.mail.bf1.yahoo.com> IHC reactions can be affected by decalcification especially if: 1- the tissue is not perfectly fixed before going into decalcification 2- decalcification is done at temperature above room temp. 3- decal solution is too acid, specially made with inorganic acids. If at all possible decalcification should be done with a chelating agent, like EDTA Ren? J. ________________________________ From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, November 9, 2012 12:34 PM Subject: [Histonet] decal affecting IHC Does decalcifying tissue? (Calex II) affect the antibody reaction for IHC in bony tissue? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Nov 9 12:00:28 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 9 12:00:32 2012 Subject: [Histonet] decal affecting IHC In-Reply-To: <1352483229.63600.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE01640B28DBBB@SBS2K8.premierlab.local> Diana I agree with Jack, formic acid is the way to go in my opinion, we use 10% formic acid routinely but have pushed it up to 20% on occasion when time was an issue. HCL can work if its controlled really well but you don't have much wiggle room, you can over decal quite quickly, formic acid is a bit more forgiving. As for Rene's comment we have seen the exact opposite with respects to the EDTA decal part. Granted we do not run a lot of IHC on EDTA decaled samples but on several occasions and with several antibodies we were able to obtain good IHC staining in formic acid decaled samples but those antibodies did not work well in EDTA decaled samples. These were not direct comparisons of the decalcification method on the same study, but on samples from different studies so other factors could have affected the results. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, November 09, 2012 10:47 AM To: Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] decal affecting IHC IHC reactions can be affected by decalcification especially if: 1- the tissue is not perfectly fixed before going into decalcification 2- decalcification is done at temperature above room temp. 3- decal solution is too acid, specially made with inorganic acids. If at all possible decalcification should be done with a chelating agent, like EDTA Ren? J. ________________________________ From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, November 9, 2012 12:34 PM Subject: [Histonet] decal affecting IHC Does decalcifying tissue (Calex II) affect the antibody reaction for IHC in bony tissue? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Fri Nov 9 12:14:49 2012 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Fri Nov 9 12:14:54 2012 Subject: [Histonet] Becky Orr Message-ID: <1352484889.20629.YahooMailNeo@web141405.mail.bf1.yahoo.com> I'm looking to contact Rebecca (Becky) Orr. Does anyone know her current email address? The one I have bounced back. ? Thanks, Andrea From jkrupp <@t> deltacollege.edu Fri Nov 9 12:49:48 2012 From: jkrupp <@t> deltacollege.edu (Jon Krupp) Date: Fri Nov 9 12:49:53 2012 Subject: [Histonet] Microtome knives Message-ID: Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College From rjbuesa <@t> yahoo.com Fri Nov 9 14:13:23 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 9 14:13:27 2012 Subject: [Histonet] Microtome knives In-Reply-To: References: Message-ID: <1352492003.36592.YahooMailNeo@web163106.mail.bf1.yahoo.com> Because?of your donated knives? you will have to buy a knives sharpener which are costly and not very easy to find. Your best option is to buy a high profile disposable blades holder (that will be cheaper), buy disposable blades and avoid all the frustrations and waste of time sharpening knives. Ren? J. ________________________________ From: Jon Krupp To: Histonet@lists.utsouthwestern.edu Sent: Friday, November 9, 2012 1:49 PM Subject: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not? histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA? 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Nov 9 14:32:13 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Nov 9 14:32:20 2012 Subject: [Histonet] Microtome knives Message-ID: Hi Jon, Depends on your cash flow. You could get a used sharpener somewhere off the web somewhere such as http://www.labx.com/v2/adsearch/resultsnew.cfm?sw=sharpener&mr=25&te=cat , or http://www.medwow.com/used-microtome-knife-sharpener-equipment/63.med but sharpening knives is a pain IMO and steel knives present more of a safety hazard. I would recommend a sharpener that uses the glass honing plates. You would also need the coarse and fine abrasives. Personally, I would opt for a low profile disposable blade holder that fits your 820. Low and high profile refer to the size (height) of the blade. We use low profile for paraffin block sectioning and high profile for cryostat sectioning. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Krupp Sent: Friday, November 09, 2012 1:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From nguy0515 <@t> gmail.com Fri Nov 9 14:48:15 2012 From: nguy0515 <@t> gmail.com (Trini Nguyen) Date: Fri Nov 9 14:48:19 2012 Subject: [Histonet] Dermatology KOH testing Message-ID: Does anyone perform KOH tests? If so, could you please email me and give me information on your process. Thanks, Trini From bluebird8081 <@t> yahoo.com Fri Nov 9 17:04:48 2012 From: bluebird8081 <@t> yahoo.com (AFirst Name Gee) Date: Fri Nov 9 17:04:53 2012 Subject: [Histonet] test Message-ID: <1352502288.25837.YahooMailNeo@web125504.mail.ne1.yahoo.com> Just testing-new subscriber From Diane.Tokugawa <@t> kp.org Fri Nov 9 17:13:01 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Nov 9 17:13:11 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 11/09/2012 and will not return until 11/15/2012. Note: For Cytology issues, please call Robin (day) at 8-421-5040, Wanda (day) 8-421-5426, or Eric (swing) 8-421-5405 For Histology issues, please call Mario (day) 8-421-4961, Barbara (swing) 8-421-4959, Bob (IHC) 8-421-4706, Kiran at 8-421-5404, or general histology client service at 8-421-5408 or Lotus Notes. From bluebird8081 <@t> yahoo.com Fri Nov 9 17:16:56 2012 From: bluebird8081 <@t> yahoo.com (AFirst Name Gee) Date: Fri Nov 9 17:16:59 2012 Subject: [Histonet] Mold Release Problems Message-ID: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> Does anyone know of a reference for the problems from using mold release?? When too much is used on the mold it eats into the block and causes the ribbons to explode on the water bath and creates an artifact in the tissue.I cannot find a reference for it or any published material about the problem but have been to seminars where it was discussed.When it is not used the problem never occurs. From joelleweaver <@t> hotmail.com Sat Nov 10 02:04:06 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Nov 10 02:04:12 2012 Subject: [Histonet] Mold Release Problems In-Reply-To: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> References: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> Message-ID: I have never seen a published reference unfortunately that I can direct you to. I do not use mold release because I have never had any trouble with this while embedding. It seems to me that if standard metal base molds are used, and they are kept clean, the blocks come out easily if they are fully hardened. However I know with those so-called "turbo" molds, release can be more difficult, and also with those plastic disposable ones ( which I also don't like, and my reason with the plastic ones is that the ones I have encountered is that they do not have a completely flat surface at the block face and so when the block is finished it is concave). But I know some people really like the mold release product and each of the different types of molds, so to each his own. So even though I don't use mold release, I suspect however, that it is some kind of silicone or other chemical that creates a residue that coats the surface of the base mold so that the paraffin is not in direct contact with the mold. It would have to be mostly non-miscible with paraffin to be able to form a barrier between the two. And, if it has any kind of solvent like materials (check out the label or MSDS if it is not a trade secret)- Or someone in histoland may know the constituents off hand? But my suspicion is that the materials are not miscible with the paraffin, and also not with anything aqueous. If you used this in excess, common sense would say that residue could cause any number of problems in the subsequent steps of microtomy and staining. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 9 Nov 2012 15:16:56 -0800 > From: bluebird8081@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mold Release Problems > > Does anyone know of a reference for the problems from using mold release? When too much is used on the mold it eats into the block and causes the ribbons to explode on the water bath and creates an artifact in the tissue.I cannot find a reference for it or any published material about the problem but have been to seminars where it was discussed.When it is not used the problem never occurs. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Sat Nov 10 05:33:15 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Sat Nov 10 07:38:08 2012 Subject: [Histonet] Mold Release Problems In-Reply-To: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> References: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> Message-ID: <357D9B9E-60E2-4B18-9EE3-81DD8297273F@medicalcenterclinic.com> What type of mold release do you use & how do you apply it to the molds? Also, what type of paraffin are you using? On Nov 9, 2012, at 5:16 PM, AFirst Name Gee wrote: > Does anyone know of a reference for the problems from using mold release? When too much is used on the mold it eats into the block and causes the ribbons to explode on the water bath and creates an artifact in the tissue.I cannot find a reference for it or any published material about the problem but have been to seminars where it was discussed.When it is not used the problem never occurs. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Nov 10 09:37:03 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Nov 10 09:37:09 2012 Subject: [Histonet] Mold Release Problems In-Reply-To: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> References: <1352503016.41043.YahooMailNeo@web125501.mail.ne1.yahoo.com> Message-ID: <1352561823.93035.YahooMailNeo@web163101.mail.bf1.yahoo.com> Mold release usually contains isopropyl alcohol and if you use too much, the alcohol will "eat into the block" as you describe and that will cause the sections to explode. Everything? you have described. If you want to use the mold release use less and better yet, do not use mold release (in reality should be called "block" release). Anyway, if you cool the block properly you do not need to use this product because a cold block will contract becoming "smaller" than the mold and in consequence will be easier to be released. Ren? J. ________________________________ From: AFirst Name Gee To: "histonet@lists.utsouthwestern.edu" Sent: Friday, November 9, 2012 6:16 PM Subject: [Histonet] Mold Release Problems Does anyone know of a reference for the problems from using mold release?? When too much is used on the mold it eats into the block and causes the ribbons to explode on the water bath and creates an artifact in the tissue.I cannot find a reference for it or any published material about the problem but have been to seminars where it was discussed.When it is not used the problem never occurs. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Sun Nov 11 03:44:40 2012 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sun Nov 11 03:44:46 2012 Subject: [Histonet] Microtome knives In-Reply-To: References: Message-ID: <1352627080.42134.YahooMailNeo@web171201.mail.ir2.yahoo.com> I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Jon Krupp A: Histonet@lists.utsouthwestern.edu Inviato: Venerd? 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not? histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA? 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sharon.harrison <@t> uwimona.edu.jm Sun Nov 11 08:22:52 2012 From: sharon.harrison <@t> uwimona.edu.jm (HARRISON,Sharon R) Date: Sun Nov 11 08:19:09 2012 Subject: [Histonet] Microtome knives In-Reply-To: <1352627080.42134.YahooMailNeo@web171201.mail.ir2.yahoo.com> References: , <1352627080.42134.YahooMailNeo@web171201.mail.ir2.yahoo.com> Message-ID: <39D05A5FD7C1334DA749CCFCE8538F873638DB5F1E@xchg1.uwimona.edu.jm> Hi All, I am Sharon Harrison, supervisor of Histopathology lab at UWI Mona in Kingston Jamaica. I agree with you that we still need to have the solid blades for the cutting of hard tissue such as cervix fibroids and bone. Hence we have to maintain some vestige of the old technology of knife sharpening, as the disposable blades are quite expensive. The disposable blades are great for biopsies and softer tissue but a real pain for getting sections of the harder tissues. Therefore I advice having both systems especially if your laboratory handles all types of tissue specimen. Sharon Harrison Chief Medical Technologist DMT, BSc, MPH ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Massimo [max_histo_00@yahoo.it] Sent: Sunday, November 11, 2012 4:44 AM To: Jon Krupp; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome knives I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Jon Krupp A: Histonet@lists.utsouthwestern.edu Inviato: Venerd? 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Sun Nov 11 15:34:30 2012 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Sun Nov 11 15:34:34 2012 Subject: [Histonet] Histologist-TMA Technician needing to relocate to CA Message-ID: I am looking to relocate to California, near the San Diago area. My wife's work is relocating her there so I am looking at my options. I am a Histologist (HT, ASCP) with 25 years of experience. I have worked in an Electron Microscopy Lab for 10 years as a Histologist and EM Tech processing and cutting paraffin and Plastics and I have 12 years of experience as a Research Histologist constructing Tissue Microarrays (TMAs). I have published 2 articles on TMA construction in the Journal of Histotechnology: Tissue Microarrays ? Beneficial Technology for Research and Histology, NSH Workshop 2001 / The Tissue Array: The Future of Histology, NSH 2003 and I have given a workshop at a NSH meeting on TMA construction. I have another article just accepted to the USCAP on new Advance TMA techniques (The Radical TMA). I am currently working as a Research Histologist and TMA technician for a Tissue BioRepository in Salt Lake City, Utah. Best regards, Thom Thom Jensen BA, HT (ASCP) TMA Technician From laurie <@t> conxis.com Mon Nov 12 11:02:37 2012 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Mon Nov 12 11:02:55 2012 Subject: [Histonet] rabbit monoclonal secondary kits Message-ID: Hello everyone. I was wondering if anyone has any suggestions for rabbit mAB secondary kits? We are currently using Biocare and Dako reagents and have recently started switching many of our optimization projects from the Dako Autostainer platform to the Bond III platform with research detection. We are seeing some of the antibodies that we are working with that have reduced signal and we are uncertain if many of these kits are set up specifically for rabbit polyclonal antibodies. Thanks for the input, Laurie Laurie Popp, HT (ASCP) research technologist From laurie <@t> conxis.com Mon Nov 12 11:24:41 2012 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Mon Nov 12 11:24:54 2012 Subject: [Histonet] rabbit monoclonal secondary kits-addendum Message-ID: <5BC42E59F49C49419F85DD863425287.MAI@accuwebhosting.biz> I should specify and I apologize for not putting this detail in the original email. I am looking for information pertaining to mouse hosted human xenograft tissues. We are not having problems when it is species specific within a host ( e.g. rabbit mAB on human or rabbit mAB on mouse. ) Thanks again, Laurie From cforster <@t> umn.edu Mon Nov 12 11:40:15 2012 From: cforster <@t> umn.edu (cforster@umn.edu) Date: Mon Nov 12 11:40:23 2012 Subject: [Histonet] rabbit monoclonal secondary kits In-Reply-To: References: Message-ID: A detection that works for rabbit should be OK. For the Bond you should be able to use the Novolink polymer (on human) samples with great results. I use it with the Biocare Nemesis and get excellent staining. You may need to adjust your protocols when you go from one automated platform to another. Colleen Forster U of MN On Nov 12 2012, laurie@conxis.com wrote: >Hello everyone. > I was wondering if anyone has any suggestions for rabbit mAB secondary kits? We are currently using Biocare and Dako reagents and have recently started switching many of our optimization projects from the Dako Autostainer platform to the Bond III platform with research detection. We are seeing some of the antibodies that we are working with that have reduced signal and we are uncertain if many of these kits are set up specifically for rabbit polyclonal antibodies. > >Thanks for the input, >Laurie > >Laurie Popp, HT (ASCP) >research technologist > > > From christina.thurby <@t> bms.com Mon Nov 12 12:11:02 2012 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon Nov 12 12:11:11 2012 Subject: [Histonet] Job opportunity-Supervisor of AP and Central Processsing In-Reply-To: <30b70c4c-c543-425c-ade9-32ca6feeefa8@ushpwbmsmhp003.one.ads.bms.com> References: <30b70c4c-c543-425c-ade9-32ca6feeefa8@ushpwbmsmhp003.one.ads.bms.com> Message-ID: Hi all, I am posting this for a friend. If interested, please see the contact e-mail listed below. Good luck! Supervisor: Anatomic Pathology & Central Processing Requirements: BS degree, 3 years experience as a Cytologist or Histologist, and 2 years of management experience. Since its founding in 1892, Deaconess Hospital, located in Evansville, Indiana has grown into an award-winning, multi-facility health system providing compassionate, high-quality health care to the entire tri-state region. Serving the communities of Southern Indiana, Southeastern Illinois and Western Kentucky, Deaconess Health System includes five hospitals, over 20 primary care locations and several specialty facilities. Deaconess Regional Laboratory offers comprehensive outreach laboratory services at multiple patient service centers throughout the Tri-State. We provide exceptional services, superior quality and rapid turnaround times. Client services include courier pickups, off-hour on-call coverage, client and patient billing and in-office phlebotomy staffing. We are a Sunquest/Powerpath computer site. The AP workload is approximately 22,000 surgicals, 35,000 imaged thinpreps, and 1,000 FNA. Our lab generated approx 2,630,000 tests during the most recent 12 months. This position has the supervisory responsibility for Anatomic Pathology, consisting of Histology, Cytology and Pathology transcription. Additionally, this position will have the responsibility of the Central Processing functions for the laboratory. Interested applicants should email Phil Gamble, Laboratory Manager at phil.gamble@deaconess.com. Christina Thurby Bristol Myers Squibb 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From NHeath <@t> Lifespan.org Mon Nov 12 13:11:13 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Mon Nov 12 13:11:20 2012 Subject: [Histonet] anybody out there working with EKI Safe-Clear?? Message-ID: <130E8991F210424096EFC6F42EA33B2409966F39@LSCOEXCH1.lsmaster.lifespan.org> Anybody using EKI Safe-Clear (xlene substitute)? I just would like to know what mounting media you are using with it. I like the stuff but, just need to find a mounting media that goes with it :) Nancy Heath, HT (ASCP) Neuropathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-9889 nheath@lifespan.org From HParker <@t> Skaggs.Net Mon Nov 12 13:31:20 2012 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Mon Nov 12 13:31:25 2012 Subject: [Histonet] Iron Stain In-Reply-To: <75ee812f-dde7-4676-a8e1-36b61208c91b@email1.skaggs.net> References: <75ee812f-dde7-4676-a8e1-36b61208c91b@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506005C19FCC5A@email1.skaggs.net> Hi All, Is there any specific reason why one iron stain kit I have recommends that you air-dry the bone marrow and blood smears after the Fe stain is completed and then coverslip them while other kits don't mention that at all. I have always stained my smears then dehydrate, clear and coverslip them like the tissue slides (at the end). Ergo Fe stain, rinse, paragon, rinse, 95% Alc, 100% Alc, xylene and coverslip. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hparker@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From rjbuesa <@t> yahoo.com Mon Nov 12 13:45:48 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 12 13:45:52 2012 Subject: [Histonet] Iron Stain In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506005C19FCC5A@email1.skaggs.net> References: <75ee812f-dde7-4676-a8e1-36b61208c91b@email1.skaggs.net> <930EB2E8DF68C544873EDD2A3D5F506005C19FCC5A@email1.skaggs.net> Message-ID: <1352749548.76456.YahooMailNeo@web163105.mail.bf1.yahoo.com> I think that instead trying to guess that "mysterious" step, you should contact the company that developed the kit and ask them. Maybe their reagents require that. Never assume! Ren? J. ________________________________ From: "Parker, Helayne" To: "'histonet@lists.utsouthwestern.edu'" Sent: Monday, November 12, 2012 2:31 PM Subject: [Histonet] Iron Stain Hi All, ? Is there any specific reason why one iron stain kit I have recommends that you air-dry the bone marrow and blood smears after the Fe stain is completed and then coverslip them while other kits don't mention that at all.? I have always stained my smears then dehydrate, clear and coverslip them like the tissue slides (at the end). Ergo Fe stain, rinse, paragon, rinse, 95% Alc, 100% Alc, xylene and coverslip.? Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hparker@skaggs.net Web: http://www.skaggs.net/ CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Norm.Burnham <@t> propath.com Mon Nov 12 15:08:34 2012 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Mon Nov 12 15:08:39 2012 Subject: [Histonet] Immediate Opening for an Experienced Histotechnologist at ProPath in Dallas, TX Message-ID: <82C7248978CB50469FD6BA68EBBEFE6708EA1F98@exchange.propathlab.com> ProPath, a progressive, CAP accredited, high volume, pathology practice, located in Dallas, Texas, is seeking a Histotechnologist. In this position you will be responsible for embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainer and coverslipper, equipment maintenance and record retention. The ideal candidate will have a high school diploma or equivalent and at least 3 years of experience as a Histotech. We prefer, HT, HTL (ASCP) registered or eligible. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, short and long term disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com ______________________________________________ Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 norm.burnham@propath.com 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell To learn more about ProPath, please visit www.propath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From pruegg <@t> ihctech.net Mon Nov 12 15:16:10 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Nov 12 15:16:09 2012 Subject: [Histonet] radiation damage to nerves Message-ID: <9394801779F942DE96BFE6422C955EC0@DESKTOP3> Does anyone have any ideas on how we might demonstrate radiation injury to peripheral nerves near the prostate (in a canine model). we would like to evaluate demyelinating changes and it has been suggested we might do this by embedding the tissue in GMA plastic and cutting 2 micron sections and doing toludine blue stain????? We are trying to avoid osmium and EM for this. Any ideas? Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net From rjbuesa <@t> yahoo.com Mon Nov 12 15:29:05 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 12 15:29:10 2012 Subject: [Histonet] radiation damage to nerves In-Reply-To: <9394801779F942DE96BFE6422C955EC0@DESKTOP3> References: <9394801779F942DE96BFE6422C955EC0@DESKTOP3> Message-ID: <1352755745.12332.YahooMailNeo@web163104.mail.bf1.yahoo.com> I think that is a good approach but I do not think that an inclusion in GMA plastic is required. A well infiltrated FFPE tissue can be sectioned at 1-2 ?m Ren? J. ________________________________ From: Patsy Ruegg To: 'Histonet' Sent: Monday, November 12, 2012 4:16 PM Subject: [Histonet] radiation damage to nerves Does anyone have any ideas on how we might demonstrate radiation injury to peripheral nerves near the prostate (in a canine model).? we would like to evaluate demyelinating changes and it has been suggested we might do this by embedding the tissue in GMA plastic and cutting 2 micron sections and doing toludine blue stain?????? We are trying to avoid osmium and EM for this. Any ideas? Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Tue Nov 13 07:39:53 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Tue Nov 13 07:40:01 2012 Subject: [Histonet] looking for two speakers from NSH Vancouver Message-ID: <130E8991F210424096EFC6F42EA33B2409967010@LSCOEXCH1.lsmaster.lifespan.org> I was wondering if anyone new the names of and how to get in touch with the two speakers that gave the 8:00 am lecture Saturday September 29th titled "Getting Good Sections of Anything"? If anybody knows out there in histo land and can help I would greatly appreciate it :) Nancy Heath, HT (ASCP) Neuropathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-9889 nheath@lifespan.org From NMP <@t> stowers.org Tue Nov 13 09:13:30 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Tue Nov 13 09:13:44 2012 Subject: [Histonet] RE: looking for two speakers from NSH Vancouver In-Reply-To: <130E8991F210424096EFC6F42EA33B2409967010@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B2409967010@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9078ED7C145@EXCHMB-02.stowers-institute.org> The instructors were Curt King and Jen Freeland Best reagards, Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Tuesday, November 13, 2012 7:40 AM To: Histonet Subject: [Histonet] looking for two speakers from NSH Vancouver I was wondering if anyone new the names of and how to get in touch with the two speakers that gave the 8:00 am lecture Saturday September 29th titled "Getting Good Sections of Anything"? If anybody knows out there in histo land and can help I would greatly appreciate it :) Nancy Heath, HT (ASCP) Neuropathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-9889 nheath@lifespan.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AHutton <@t> dh.org Tue Nov 13 10:00:10 2012 From: AHutton <@t> dh.org (Hutton, Allison) Date: Tue Nov 13 10:03:05 2012 Subject: [Histonet] staffing numbers Message-ID: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison From bmillerman <@t> yahoo.com Tue Nov 13 10:12:06 2012 From: bmillerman <@t> yahoo.com (b millerman) Date: Tue Nov 13 10:12:15 2012 Subject: [Histonet] DAPI turning orange Message-ID: <1352823126.88193.YahooMailClassic@web182201.mail.bf1.yahoo.com> Has anyone had a problem with DAPI looking beautifully blue nuclear staining and then have it orange the next day? ? What is the problem and the cure? ? Thank you ? Beth Millerman, Stiefel Labs From zonked <@t> gmail.com Tue Nov 13 10:52:12 2012 From: zonked <@t> gmail.com (z o n k e d) Date: Tue Nov 13 10:52:20 2012 Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Message-ID: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion From chapcl <@t> yahoo.com Tue Nov 13 11:00:24 2012 From: chapcl <@t> yahoo.com (Will Chappell) Date: Tue Nov 13 11:00:33 2012 Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: <4BC568E9-6A48-47BD-885E-3B760FA7CB58@yahoo.com> Nope, sorry. All your fat is dissolved. Sent from my iPhone On Nov 13, 2012, at 8:52 AM, z o n k e d wrote: > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail2 <@t> gmail.com Tue Nov 13 11:10:16 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Tue Nov 13 11:10:23 2012 Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't help. You can't replace the fat. Rena Fail On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d wrote: > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From zonked <@t> gmail.com Tue Nov 13 11:11:16 2012 From: zonked <@t> gmail.com (z o n k e d) Date: Tue Nov 13 11:11:24 2012 Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: <4BC568E9-6A48-47BD-885E-3B760FA7CB58@yahoo.com> References: <4BC568E9-6A48-47BD-885E-3B760FA7CB58@yahoo.com> Message-ID: Ah, I knew it. :( Thank you. Zoe On Tuesday, November 13, 2012, Will Chappell wrote: > Nope, sorry. All your fat is dissolved. > > Sent from my iPhone > > On Nov 13, 2012, at 8:52 AM, z o n k e d > > wrote: > > > Hello Histonetters, > > > > First time writer, long time reader. I'm a newbie tech in academia and I > > was given a simple task which I think I pretty much screwed up. > > > > I should have embedded half of a mouse liver in paraffin for microtome > > sectioning while the other half should have been embedded in OCT for > > cryosectioning (for oil red o). I made the mistake last night of placing > > both liver halves into the tissue processor. The liver I intended for OCT > > embedding is now hard as wax. Is there any way to deparaffinize processed > > organs and may I embed them in OCT for proper cryosectioning? I imagine > > that the liver would get dehydrated, I would get crappy sections, and Oil > > Red O won't work. > > > > Any suggestions are welcome. > > > > Thank you so much, > > > > Zoe W. > > > > > > -- > > "It costs nothing to say something kind. Even less to shut up > altogether." > > > > --Nathan Fillion > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion From JMacDonald <@t> mtsac.edu Tue Nov 13 11:14:31 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 13 11:14:40 2012 Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d To: "histonet@lists.utsouthwestern.edu" Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-bounces@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Tue Nov 13 11:28:43 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Nov 13 11:28:57 2012 Subject: [Histonet] Xpress Question In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395898A83@chimsx08.CHI.catholichealth.net> We are going to go w the Xpress 120. I have worked with it before, but stuff changes. What are Xpress users on histonet doing to clean the baskets and handles after use? We formerly used a dishwasher, but that is not a favorite of ouor plumbing folks fearing that we will clog drains w wax. I guess they think we are going to be pouring gallons of molten wax through the system. Any help would be great help! -Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From avogaro <@t> science.unitn.it Tue Nov 13 11:32:09 2012 From: avogaro <@t> science.unitn.it (Laura Avogaro) Date: Tue Nov 13 11:32:15 2012 Subject: [Histonet] Problem with cardiomyocytes staining Message-ID: <3064.192.168.178.78.1352827929.squirrel@www.science.unitn.it> Dear Histonetters, I am new in histology so I ask if someone have experience with cardiac tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 microns thickness) but in some sections I noted cardiomyocytes colored brown (instead of pale yellow).I wonder if it is probably due to the thickness of the section or something alse(unfortunately I use an old microtome whose probably fails to work in the correct way). Thank you in advance Best regards Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Italy From jqb7 <@t> cdc.gov Tue Nov 13 11:36:36 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Nov 13 11:36:45 2012 Subject: [Histonet] RE: Xpress Question In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395898A83@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF9395898A83@chimsx08.CHI.catholichealth.net> Message-ID: We have a great, small, portable dishwasher that we use to wash the baskets as well as our coplin jars. Just make sure you wipe all the molten paraffin off the baskets thoroughly before you wash them. We have had no problems at all re: plumbing. I can send info. re: the dishwasher. At the time we bought it it was under $200.00 and has been a great workhouse. It's not even a lab dishwasher! Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, November 13, 2012 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xpress Question We are going to go w the Xpress 120. I have worked with it before, but stuff changes. What are Xpress users on histonet doing to clean the baskets and handles after use? We formerly used a dishwasher, but that is not a favorite of ouor plumbing folks fearing that we will clog drains w wax. I guess they think we are going to be pouring gallons of molten wax through the system. Any help would be great help! -Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zonked <@t> gmail.com Tue Nov 13 11:43:45 2012 From: zonked <@t> gmail.com (z o n k e d) Date: Tue Nov 13 11:43:53 2012 Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and ceroid > can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d 'zonked@gmail.com');>> > To: "histonet@lists.utsouthwestern.edu 'histonet@lists.utsouthwestern.edu');>" > > > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: histonet-bounces@lists.utsouthwestern.edu > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu 'Histonet@lists.utsouthwestern.edu');> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion From gmartin <@t> marshallmedical.org Tue Nov 13 12:07:36 2012 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Nov 13 12:07:45 2012 Subject: [Histonet] On call Message-ID: <6ED9D4252F278841A0593D3D788AF24C179597D6@mailsvr.MARSHMED.local> This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary From LPaveli1 <@t> hurleymc.com Tue Nov 13 12:54:05 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Nov 13 12:54:13 2012 Subject: [Histonet] RE: staffing numbers In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF5692BC@EXCHANGEMB1.hmc.hurleymc.com> We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Hutton, Allison [AHutton@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Nov 13 13:01:14 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 13 13:01:20 2012 Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: if there was fat replacement, such as cirrhosis, you will see it in the morphology. From: z o n k e d To: Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Date: 11/13/2012 09:43 AM Subject: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d To: "histonet@lists.utsouthwestern.edu" < histonet@lists.utsouthwestern.edu> Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-bounces@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion From zonked <@t> gmail.com Tue Nov 13 13:41:06 2012 From: zonked <@t> gmail.com (z o n k e d) Date: Tue Nov 13 13:41:10 2012 Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: Thank you all for your helpful responses! I will go ahead and carry on with paraffin sectioning and stain with H&E as planned. As for the Oil Red O, I'll try that too and see what happens. (Still amazed at how amazing Histonet is and how helpful you all are! Thank you very much for all your responses!) On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald wrote: > if there was fat replacement, such as cirrhosis, you will see it in the > morphology. > > > > From: z o n k e d > To: Jennifer MacDonald > Cc: "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu>, " > histonet-bounces@lists.utsouthwestern.edu" < > histonet-bounces@lists.utsouthwestern.edu> > Date: 11/13/2012 09:43 AM > Subject: Re: Help! Liver mistakenly processed in paraffin (had to > be in OCT instead)! > ------------------------------ > > > > We just wanted to see general lipids, nothing in particular. This mouse > died unexpectedly and may have been part of a group that was put on a high > fat or high bile acid diet and we just wanted to see what happened. > > On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and ceroid > can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d <*zonked@gmail.com*> > To: "*histonet@lists.utsouthwestern.edu*" <* > histonet@lists.utsouthwestern.edu*> > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: *histonet-bounces@lists.utsouthwestern.edu* > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list* > **Histonet@lists.utsouthwestern.edu** > **http://lists.utsouthwestern.edu/mailman/listinfo/histonet* > > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion From rjbuesa <@t> yahoo.com Tue Nov 13 13:42:46 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 13 13:42:49 2012 Subject: [Histonet] staffing numbers In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> Message-ID: <1352835766.11849.YahooMailNeo@web163102.mail.bf1.yahoo.com> Allison: The information you need is in at http://histosearch.com/rene.html Ren? J. ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:00 AM Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing.? I would like to know the number of cases done per year and your number of histotechs.? Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 13 13:47:42 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 13 13:47:45 2012 Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: <1352836062.62198.YahooMailNeo@web163106.mail.bf1.yahoo.com> You really screwed it up! When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain. Try to get another piece. Anything you will try will not render good cryosections and no fat staining. Ren? J. ________________________________ From: z o n k e d To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 13, 2012 11:52 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." ? ? --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LLoss <@t> dermwisconsin.com Tue Nov 13 15:21:01 2012 From: LLoss <@t> dermwisconsin.com (Lee Loss) Date: Tue Nov 13 15:21:07 2012 Subject: [Histonet] liftin tissue Message-ID: <2B99CB40EC5CC7409888A2961CBF96880C32A0FC@EX2010.DERM.LOCAL> I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From amosbrooks <@t> gmail.com Tue Nov 13 16:18:51 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Nov 13 16:18:54 2012 Subject: [Histonet] Problem with cardiomyocytes staining Message-ID: Hi Laura, I do this stain very frequently. It can be finickey. You should make very sure the pH is below 2.5. It could be that you have an old solution and over time these tend to drift toward a neutral pH. This will affect the staining. Sometimes the solutions need to be discarded and either re-made or have new purchased. Best of luck, Amos On Tue, Nov 13, 2012 at 1:00 PM, wrote: > Message: 18 > Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET) > From: "Laura Avogaro" > Subject: [Histonet] Problem with cardiomyocytes staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > <3064.192.168.178.78.1352827929.squirrel@www.science.unitn.it> > Content-Type: text/plain;charset=iso-8859-1 > > Dear Histonetters, > I am new in histology so I ask if someone have experience with cardiac > tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 > microns thickness) but in some sections I noted cardiomyocytes colored > brown (instead of pale yellow).I wonder if it is probably due to the > thickness of the section or something alse(unfortunately I use an old > microtome whose probably fails to work in the correct way). > Thank you in advance > Best regards > > Laura > From sadey <@t> hotmail.ca Tue Nov 13 17:14:00 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Tue Nov 13 17:14:04 2012 Subject: [Histonet] Cassette and slide labelling systems Message-ID: Hi Everyone:I'm looking for opinions on cassette and slide labelling systems. Please share your likes and dislikes.ThanksSheila p.s Vendors Do Not call me over this email. That's very annoying From ree3 <@t> leicester.ac.uk Wed Nov 14 03:00:11 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Nov 14 03:00:56 2012 Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101A7F49AC81A@EXC-MBX3.cfs.le.ac.uk> Perhaps all is not lost as you will be able to see on a H@E the spaces reluctantly vacated by the lipids. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of z o n k e d Sent: 13 November 2012 17:44 To: Jennifer MacDonald Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and > ceroid can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d 'zonked@gmail.com');>> > To: "histonet@lists.utsouthwestern.edu 'histonet@lists.utsouthwestern.edu');>" > 'histonet@lists.utsouthwestern.edu');> > > > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: histonet-bounces@lists.utsouthwestern.edu > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and > I was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of > placing both liver halves into the tissue processor. The liver I > intended for OCT embedding is now hard as wax. Is there any way to > deparaffinize processed organs and may I embed them in OCT for proper > cryosectioning? I imagine that the liver would get dehydrated, I would > get crappy sections, and Oil Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu 'Histonet@lists.utsouthwestern.edu');> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jean-philippe.berteau <@t> tu-harburg.de Wed Nov 14 04:07:35 2012 From: jean-philippe.berteau <@t> tu-harburg.de (Jean-Philippe Berteau) Date: Wed Nov 14 04:09:54 2012 Subject: [Histonet] Technovit 9100 new fails to polymerize Message-ID: <001001cdc24f$deaa7390$9bff5ab0$@tu-harburg.de> Hello, I got the same problem, how did you solve it ? Best regards Jean-Philippe From jean-philippe.berteau <@t> tu-harburg.de Wed Nov 14 04:13:58 2012 From: jean-philippe.berteau <@t> tu-harburg.de (Jean-Philippe Berteau) Date: Wed Nov 14 04:14:05 2012 Subject: [Histonet] Technovit 9100 new fails to polymerize Message-ID: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de> Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, From TMcNemar <@t> lmhealth.org Wed Nov 14 04:58:25 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Nov 14 04:58:17 2012 Subject: [Histonet] RE: staffing numbers In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE687@dhmail.dhorg.org> Message-ID: Allison, We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep. We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From NMP <@t> stowers.org Wed Nov 14 06:43:56 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Nov 14 06:44:08 2012 Subject: [Histonet] Technovit 9100 new fails to polymerize In-Reply-To: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de> References: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9078ED7C148@EXCHMB-02.stowers-institute.org> We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau Sent: Wednesday, November 14, 2012 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 new fails to polymerize Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Wed Nov 14 09:26:26 2012 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Nov 14 09:26:32 2012 Subject: [Histonet] TBS Slide Printer Message-ID: I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks. Christie From vperez <@t> pathreflab.com Wed Nov 14 09:43:00 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Wed Nov 14 09:42:20 2012 Subject: [Histonet] RE: liftin tissue In-Reply-To: <2B99CB40EC5CC7409888A2961CBF96880C32A0FC@EX2010.DERM.LOCAL> References: <2B99CB40EC5CC7409888A2961CBF96880C32A0FC@EX2010.DERM.LOCAL> Message-ID: We use Mount quick media, which comes with a procedure for section transfer.... Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, November 13, 2012 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] liftin tissue I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmconway <@t> usgs.gov Wed Nov 14 09:43:56 2012 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Wed Nov 14 09:44:26 2012 Subject: [Histonet] Zinc formalin as primary fixative for TEM Message-ID: Hello everyone, A colleague has tissues which are fixed in zinc formalin and wants to examine them by transmission electron microscopy. Any thoughts regarding the use of this fixative for EM? Thanks in advance, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From pitts.jaclyn <@t> gmail.com Wed Nov 14 09:50:34 2012 From: pitts.jaclyn <@t> gmail.com (Jaclyn Pitts) Date: Wed Nov 14 09:50:41 2012 Subject: [Histonet] H&E Question Message-ID: Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie From peter.craven <@t> nhs.net Wed Nov 14 09:52:11 2012 From: peter.craven <@t> nhs.net (Craven Peter (NHS HIGHLAND)) Date: Wed Nov 14 09:53:32 2012 Subject: [Histonet] H&E Question In-Reply-To: <20121114155108.A0FCF45882B@nhs-pd1e-esg009.ad1.nhs.net> References: <20121114155108.A0FCF45882B@nhs-pd1e-esg009.ad1.nhs.net> Message-ID: <20121114155324.DDD98448D6F@nhs-pd1e-esg101.ad1.nhs.net> Jaclyn it sounds daft but check the pH of your Eosin we found this can be strongly acidic. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jaclyn@gmail.com] Sent: 14 November 2012 03:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From TGoins <@t> mt.gov Wed Nov 14 10:02:57 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Nov 14 10:03:11 2012 Subject: [Histonet] H&E Question In-Reply-To: References: Message-ID: Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Nov 14 10:11:34 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Nov 14 10:11:37 2012 Subject: [Histonet] Histology Supervisor Needed in Atlanta. Can you help? Message-ID: <046c01cdc282$b7f69520$27e3bf60$@earthlink.net> Hi Histonetters! I hope everybody is having a great day. I wanted to drop you a quick line to ask for help because I am currently recruiting for one of my best clients located in Atlanta, GA. We are looking for an experienced Histology Supervisor. We are looking for someone who is ASCP certified with at least 3 years of histology and 2 years of experience supervising a histology lab. They are offering a great salary, terrific benefits, the stability of a large company and a great group of people to work with. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at relia1@earthlink.net or toll free at 866-607-3542. Remember if I hire someone you refer you will receive a referral bonus! Thank you, Pam - 866-607-3542 (866-60RELIA) - Toll Free Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa <@t> yahoo.com Wed Nov 14 10:20:00 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 14 10:20:11 2012 Subject: [Histonet] H&E Question In-Reply-To: References: Message-ID: <1352910000.32673.YahooMailNeo@web163101.mail.bf1.yahoo.com> I never heard of "Azer Scientific hematoxylin" but if you can leave the sections in it during 12 minutes and it is still "weak" for your pathologist, it seems that it is a "progressive" hematoxylin, of the Mayer type. Progressive hematoxylins are somewhat tricky and have to be very fresh to work well. I have always used and preferred "regressive" hematoxylins of the Harris' type. With them you are in control of the staining when you differentiate them up to the strength?proffered by your pathologist. My advise, since it seems that you cannot satisfy your pathologist's demands,is to switch to a Harris type hematoxylin and stop fiddling with the hematoxylin you are using. I always used and preferred Harris by Richard Alan. Switch and your problems will be over. Ren? J. ________________________________ From: Jaclyn Pitts To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 10:50 AM Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5? min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Wed Nov 14 11:34:38 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Nov 14 11:34:46 2012 Subject: [Histonet] H&E Question In-Reply-To: References: , Message-ID: <89F4666A496DC949A819ECC40E11C867BF569441@EXCHANGEMB1.hmc.hurleymc.com> I know this is out of the box, but it happened to me so I thought I'd pass this along. We had the same issue with our chief pathologist.......hematoxylin was too light. We tried everything, but she still complained!! Then we diluted our Eosin Y 1:1 with 95% alcohol and then she was happy. Crazy, I know, but something to think about! Best of luck, and please let us know your findings! Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Goins, Tresa [TGoins@mt.gov] Sent: Wednesday, November 14, 2012 11:02 AM To: Jaclyn Pitts; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Question Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Nov 14 11:35:59 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Nov 14 11:37:49 2012 Subject: [Histonet] H&E Question In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF569441@EXCHANGEMB1.hmc.hurleymc.com> References: , <89F4666A496DC949A819ECC40E11C867BF569441@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916434051C3@IBMB7Exchange.digestivespecialists.com> I second this one! Been there! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, November 14, 2012 12:35 PM To: Goins, Tresa; Jaclyn Pitts; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Question I know this is out of the box, but it happened to me so I thought I'd pass this along. We had the same issue with our chief pathologist.......hematoxylin was too light. We tried everything, but she still complained!! Then we diluted our Eosin Y 1:1 with 95% alcohol and then she was happy. Crazy, I know, but something to think about! Best of luck, and please let us know your findings! Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Goins, Tresa [TGoins@mt.gov] Sent: Wednesday, November 14, 2012 11:02 AM To: Jaclyn Pitts; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Question Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dgoodwin <@t> rwjuhh.edu Wed Nov 14 11:53:09 2012 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Wed Nov 14 11:53:26 2012 Subject: [Histonet] RE: Staffing In-Reply-To: <1352910427.430762@messagescreen.rwjham.net> References: <1352910427.430762@messagescreen.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C729D2C44A47@HAMEXMBA.rwjham.local> We processed about 30,000 blocks in 2011 with 2 FTE Histotechs and 1 PRN tech. I also work the bench about 70% of the time, and we have a FT tech assistant. FYI, there was a study by Kohl et al: "The CAP Subject: [Histonet] On call To: Message-ID: <6ED9D4252F278841A0593D3D788AF24C179597D6@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary ------------------------------ Message: 2 Date: Tue, 13 Nov 2012 18:54:05 +0000 From: Lynette Pavelich Subject: [Histonet] RE: staffing numbers To: "Hutton, Allison" , "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF5692BC@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Hutton, Allison [AHutton@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 13 Nov 2012 11:01:14 -0800 From: Jennifer MacDonald Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="US-ASCII" if there was fat replacement, such as cirrhosis, you will see it in the morphology. From: z o n k e d To: Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Date: 11/13/2012 09:43 AM Subject: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d To: "histonet@lists.utsouthwestern.edu" < histonet@lists.utsouthwestern.edu> Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-bounces@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion ------------------------------ Message: 4 Date: Tue, 13 Nov 2012 14:41:06 -0500 From: z o n k e d Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: Jennifer MacDonald , ryaskovich@dir.nidcr.nih.gov Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank you all for your helpful responses! I will go ahead and carry on with paraffin sectioning and stain with H&E as planned. As for the Oil Red O, I'll try that too and see what happens. (Still amazed at how amazing Histonet is and how helpful you all are! Thank you very much for all your responses!) On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald wrote: > if there was fat replacement, such as cirrhosis, you will see it in the > morphology. > > > > From: z o n k e d > To: Jennifer MacDonald > Cc: "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu>, " > histonet-bounces@lists.utsouthwestern.edu" < > histonet-bounces@lists.utsouthwestern.edu> > Date: 11/13/2012 09:43 AM > Subject: Re: Help! Liver mistakenly processed in paraffin (had to > be in OCT instead)! > ------------------------------ > > > > We just wanted to see general lipids, nothing in particular. This mouse > died unexpectedly and may have been part of a group that was put on a high > fat or high bile acid diet and we just wanted to see what happened. > > On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and ceroid > can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d <*zonked@gmail.com*> > To: "*histonet@lists.utsouthwestern.edu*" <* > histonet@lists.utsouthwestern.edu*> > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: *histonet-bounces@lists.utsouthwestern.edu* > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list* > **Histonet@lists.utsouthwestern.edu** > **http://lists.utsouthwestern.edu/mailman/listinfo/histonet* > > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion ------------------------------ Message: 5 Date: Tue, 13 Nov 2012 11:42:46 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] staffing numbers To: "Hutton, Allison" , "histonet@lists.utsouthwestern.edu" Message-ID: <1352835766.11849.YahooMailNeo@web163102.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Allison: The information you need is in at http://histosearch.com/rene.html Ren? J. ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:00 AM Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing.? I would like to know the number of cases done per year and your number of histotechs.? Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 13 Nov 2012 11:47:42 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d , "histonet@lists.utsouthwestern.edu" Message-ID: <1352836062.62198.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You really screwed it up! When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain. Try to get another piece. Anything you will try will not render good cryosections and no fat staining. Ren? J. ________________________________ From: z o n k e d To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 13, 2012 11:52 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." ? ? --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 13 Nov 2012 21:21:01 +0000 From: Lee Loss Subject: [Histonet] liftin tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: <2B99CB40EC5CC7409888A2961CBF96880C32A0FC@EX2010.DERM.LOCAL> Content-Type: text/plain; charset="us-ascii" I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ------------------------------ Message: 8 Date: Tue, 13 Nov 2012 17:18:51 -0500 From: Amos Brooks Subject: [Histonet] Problem with cardiomyocytes staining To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Laura, I do this stain very frequently. It can be finickey. You should make very sure the pH is below 2.5. It could be that you have an old solution and over time these tend to drift toward a neutral pH. This will affect the staining. Sometimes the solutions need to be discarded and either re-made or have new purchased. Best of luck, Amos On Tue, Nov 13, 2012 at 1:00 PM, wrote: > Message: 18 > Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET) > From: "Laura Avogaro" > Subject: [Histonet] Problem with cardiomyocytes staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > <3064.192.168.178.78.1352827929.squirrel@www.science.unitn.it> > Content-Type: text/plain;charset=iso-8859-1 > > Dear Histonetters, > I am new in histology so I ask if someone have experience with cardiac > tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 > microns thickness) but in some sections I noted cardiomyocytes colored > brown (instead of pale yellow).I wonder if it is probably due to the > thickness of the section or something alse(unfortunately I use an old > microtome whose probably fails to work in the correct way). > Thank you in advance > Best regards > > Laura > ------------------------------ Message: 9 Date: Tue, 13 Nov 2012 18:14:00 -0500 From: Sheila Adey Subject: [Histonet] Cassette and slide labelling systems To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Everyone:I'm looking for opinions on cassette and slide labelling systems. Please share your likes and dislikes.ThanksSheila p.s Vendors Do Not call me over this email. That's very annoying ------------------------------ Message: 10 Date: Wed, 14 Nov 2012 09:00:11 +0000 From: "Edwards, Richard E." Subject: RE: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: 'z o n k e d' , Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A101A7F49AC81A@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Perhaps all is not lost as you will be able to see on a H@E the spaces reluctantly vacated by the lipids. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of z o n k e d Sent: 13 November 2012 17:44 To: Jennifer MacDonald Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and > ceroid can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d 'zonked@gmail.com');>> > To: "histonet@lists.utsouthwestern.edu 'histonet@lists.utsouthwestern.edu');>" > 'histonet@lists.utsouthwestern.edu');> > > > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: histonet-bounces@lists.utsouthwestern.edu > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and > I was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of > placing both liver halves into the tissue processor. The liver I > intended for OCT embedding is now hard as wax. Is there any way to > deparaffinize processed organs and may I embed them in OCT for proper > cryosectioning? I imagine that the liver would get dehydrated, I would > get crappy sections, and Oil Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu 'Histonet@lists.utsouthwestern.edu');> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 14 Nov 2012 11:07:35 +0100 From: "Jean-Philippe Berteau" Subject: [Histonet] Technovit 9100 new fails to polymerize To: Message-ID: <001001cdc24f$deaa7390$9bff5ab0$@tu-harburg.de> Content-Type: text/plain; charset="us-ascii" Hello, I got the same problem, how did you solve it ? Best regards Jean-Philippe ------------------------------ Message: 12 Date: Wed, 14 Nov 2012 11:13:58 +0100 From: "Jean-Philippe Berteau" Subject: [Histonet] Technovit 9100 new fails to polymerize To: Message-ID: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de> Content-Type: text/plain; charset="us-ascii" Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ------------------------------ Message: 13 Date: Wed, 14 Nov 2012 05:58:25 -0500 From: Tom McNemar Subject: [Histonet] RE: staffing numbers To: "'Hutton, Allison'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Allison, We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep. We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 14 Date: Wed, 14 Nov 2012 06:43:56 -0600 From: "Marsh, Nannette" Subject: RE: [Histonet] Technovit 9100 new fails to polymerize To: "'Jean-Philippe Berteau'" , "histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9078ED7C148@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau Sent: Wednesday, November 14, 2012 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 new fails to polymerize Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 14 Nov 2012 15:26:26 +0000 From: CHRISTIE GOWAN Subject: [Histonet] TBS Slide Printer To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks. Christie ------------------------------ Message: 16 Date: Wed, 14 Nov 2012 09:43:00 -0600 From: Vanessa Perez Subject: [Histonet] RE: liftin tissue To: Lee Loss , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use Mount quick media, which comes with a procedure for section transfer.... Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, November 13, 2012 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] liftin tissue I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 14 Nov 2012 07:43:56 -0800 From: Carla M Conway Subject: [Histonet] Zinc formalin as primary fixative for TEM To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello everyone, A colleague has tissues which are fixed in zinc formalin and wants to examine them by transmission electron microscopy. Any thoughts regarding the use of this fixative for EM? Thanks in advance, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov ------------------------------ Message: 18 Date: Wed, 14 Nov 2012 09:50:34 -0600 From: Jaclyn Pitts Subject: [Histonet] H&E Question To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ------------------------------ Message: 19 Date: Wed, 14 Nov 2012 15:52:11 +0000 From: "Craven Peter (NHS HIGHLAND)" Subject: RE: [Histonet] H&E Question To: Jaclyn Pitts , "histonet@lists.utsouthwestern.edu" Message-ID: <20121114155324.DDD98448D6F@nhs-pd1e-esg101.ad1.nhs.net> Content-Type: text/plain; charset="us-ascii" Jaclyn it sounds daft but check the pH of your Eosin we found this can be strongly acidic. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jaclyn@gmail.com] Sent: 14 November 2012 03:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** ------------------------------ Message: 20 Date: Wed, 14 Nov 2012 16:02:57 +0000 From: "Goins, Tresa" Subject: RE: [Histonet] H&E Question To: Jaclyn Pitts , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 14 Nov 2012 11:11:34 -0500 From: "Pam Barker" Subject: [Histonet] Histology Supervisor Needed in Atlanta. Can you help? To: "Histonet" Message-ID: <046c01cdc282$b7f69520$27e3bf60$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I hope everybody is having a great day. I wanted to drop you a quick line to ask for help because I am currently recruiting for one of my best clients located in Atlanta, GA. We are looking for an experienced Histology Supervisor. We are looking for someone who is ASCP certified with at least 3 years of histology and 2 years of experience supervising a histology lab. They are offering a great salary, terrific benefits, the stability of a large company and a great group of people to work with. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at relia1@earthlink.net or toll free at 866-607-3542. Remember if I hire someone you refer you will receive a referral bonus! Thank you, Pam - 866-607-3542 (866-60RELIA) - Toll Free Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 22 Date: Wed, 14 Nov 2012 08:20:00 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] H&E Question To: Jaclyn Pitts , "histonet@lists.utsouthwestern.edu" Message-ID: <1352910000.32673.YahooMailNeo@web163101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I never heard of "Azer Scientific hematoxylin" but if you can leave the sections in it during 12 minutes and it is still "weak" for your pathologist, it seems that it is a "progressive" hematoxylin, of the Mayer type. Progressive hematoxylins are somewhat tricky and have to be very fresh to work well. I have always used and preferred "regressive" hematoxylins of the Harris' type. With them you are in control of the staining when you differentiate them up to the strength?proffered by your pathologist. My advise, since it seems that you cannot satisfy your pathologist's demands,is to switch to a Harris type hematoxylin and stop fiddling with the hematoxylin you are using. I always used and preferred Harris by Richard Alan. Switch and your problems will be over. Ren? J. ________________________________ From: Jaclyn Pitts To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 10:50 AM Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5? min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 108, Issue 18 ***************************************** From dgoodwin <@t> rwjuhh.edu Wed Nov 14 11:57:37 2012 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Wed Nov 14 11:57:52 2012 Subject: [Histonet] RE: H&E staining In-Reply-To: <1352910427.430762@messagescreen.rwjham.net> References: <1352910427.430762@messagescreen.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C729D2C44A48@HAMEXMBA.rwjham.local> Jackie: If you are using tap water to rinse, check the pH. Acidic tap water can lighten the Hematoxylin staining. Diana Goodwin Histology Supervisor RWJUHH 609-631-6996 dgoodwin@rwjuhh.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 108, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. On call (Martin, Gary) 2. RE: staffing numbers (Lynette Pavelich) 3. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Jennifer MacDonald) 4. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (z o n k e d) 5. Re: staffing numbers (Rene J Buesa) 6. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Rene J Buesa) 7. liftin tissue (Lee Loss) 8. Problem with cardiomyocytes staining (Amos Brooks) 9. Cassette and slide labelling systems (Sheila Adey) 10. RE: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Edwards, Richard E.) 11. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 12. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 13. RE: staffing numbers (Tom McNemar) 14. RE: Technovit 9100 new fails to polymerize (Marsh, Nannette) 15. TBS Slide Printer (CHRISTIE GOWAN) 16. RE: liftin tissue (Vanessa Perez) 17. Zinc formalin as primary fixative for TEM (Carla M Conway) 18. H&E Question (Jaclyn Pitts) 19. RE: H&E Question (Craven Peter (NHS HIGHLAND)) 20. RE: H&E Question (Goins, Tresa) 21. Histology Supervisor Needed in Atlanta. Can you help? (Pam Barker) 22. Re: H&E Question (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 13 Nov 2012 10:07:36 -0800 From: "Martin, Gary" Subject: [Histonet] On call To: Message-ID: <6ED9D4252F278841A0593D3D788AF24C179597D6@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary ------------------------------ Message: 2 Date: Tue, 13 Nov 2012 18:54:05 +0000 From: Lynette Pavelich Subject: [Histonet] RE: staffing numbers To: "Hutton, Allison" , "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF5692BC@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Hutton, Allison [AHutton@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 13 Nov 2012 11:01:14 -0800 From: Jennifer MacDonald Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="US-ASCII" if there was fat replacement, such as cirrhosis, you will see it in the morphology. From: z o n k e d To: Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Date: 11/13/2012 09:43 AM Subject: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d To: "histonet@lists.utsouthwestern.edu" < histonet@lists.utsouthwestern.edu> Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-bounces@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion ------------------------------ Message: 4 Date: Tue, 13 Nov 2012 14:41:06 -0500 From: z o n k e d Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: Jennifer MacDonald , ryaskovich@dir.nidcr.nih.gov Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank you all for your helpful responses! I will go ahead and carry on with paraffin sectioning and stain with H&E as planned. As for the Oil Red O, I'll try that too and see what happens. (Still amazed at how amazing Histonet is and how helpful you all are! Thank you very much for all your responses!) On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald wrote: > if there was fat replacement, such as cirrhosis, you will see it in the > morphology. > > > > From: z o n k e d > To: Jennifer MacDonald > Cc: "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu>, " > histonet-bounces@lists.utsouthwestern.edu" < > histonet-bounces@lists.utsouthwestern.edu> > Date: 11/13/2012 09:43 AM > Subject: Re: Help! Liver mistakenly processed in paraffin (had to > be in OCT instead)! > ------------------------------ > > > > We just wanted to see general lipids, nothing in particular. This mouse > died unexpectedly and may have been part of a group that was put on a high > fat or high bile acid diet and we just wanted to see what happened. > > On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and ceroid > can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d <*zonked@gmail.com*> > To: "*histonet@lists.utsouthwestern.edu*" <* > histonet@lists.utsouthwestern.edu*> > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: *histonet-bounces@lists.utsouthwestern.edu* > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list* > **Histonet@lists.utsouthwestern.edu** > **http://lists.utsouthwestern.edu/mailman/listinfo/histonet* > > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion ------------------------------ Message: 5 Date: Tue, 13 Nov 2012 11:42:46 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] staffing numbers To: "Hutton, Allison" , "histonet@lists.utsouthwestern.edu" Message-ID: <1352835766.11849.YahooMailNeo@web163102.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Allison: The information you need is in at http://histosearch.com/rene.html Ren? J. ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:00 AM Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing.? I would like to know the number of cases done per year and your number of histotechs.? Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 13 Nov 2012 11:47:42 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d , "histonet@lists.utsouthwestern.edu" Message-ID: <1352836062.62198.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You really screwed it up! When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain. Try to get another piece. Anything you will try will not render good cryosections and no fat staining. Ren? J. ________________________________ From: z o n k e d To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 13, 2012 11:52 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." ? ? --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 13 Nov 2012 21:21:01 +0000 From: Lee Loss Subject: [Histonet] liftin tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: <2B99CB40EC5CC7409888A2961CBF96880C32A0FC@EX2010.DERM.LOCAL> Content-Type: text/plain; charset="us-ascii" I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ------------------------------ Message: 8 Date: Tue, 13 Nov 2012 17:18:51 -0500 From: Amos Brooks Subject: [Histonet] Problem with cardiomyocytes staining To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Laura, I do this stain very frequently. It can be finickey. You should make very sure the pH is below 2.5. It could be that you have an old solution and over time these tend to drift toward a neutral pH. This will affect the staining. Sometimes the solutions need to be discarded and either re-made or have new purchased. Best of luck, Amos On Tue, Nov 13, 2012 at 1:00 PM, wrote: > Message: 18 > Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET) > From: "Laura Avogaro" > Subject: [Histonet] Problem with cardiomyocytes staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > <3064.192.168.178.78.1352827929.squirrel@www.science.unitn.it> > Content-Type: text/plain;charset=iso-8859-1 > > Dear Histonetters, > I am new in histology so I ask if someone have experience with cardiac > tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 > microns thickness) but in some sections I noted cardiomyocytes colored > brown (instead of pale yellow).I wonder if it is probably due to the > thickness of the section or something alse(unfortunately I use an old > microtome whose probably fails to work in the correct way). > Thank you in advance > Best regards > > Laura > ------------------------------ Message: 9 Date: Tue, 13 Nov 2012 18:14:00 -0500 From: Sheila Adey Subject: [Histonet] Cassette and slide labelling systems To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Everyone:I'm looking for opinions on cassette and slide labelling systems. Please share your likes and dislikes.ThanksSheila p.s Vendors Do Not call me over this email. That's very annoying ------------------------------ Message: 10 Date: Wed, 14 Nov 2012 09:00:11 +0000 From: "Edwards, Richard E." Subject: RE: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: 'z o n k e d' , Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A101A7F49AC81A@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Perhaps all is not lost as you will be able to see on a H@E the spaces reluctantly vacated by the lipids. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of z o n k e d Sent: 13 November 2012 17:44 To: Jennifer MacDonald Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and > ceroid can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d 'zonked@gmail.com');>> > To: "histonet@lists.utsouthwestern.edu 'histonet@lists.utsouthwestern.edu');>" > 'histonet@lists.utsouthwestern.edu');> > > > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: histonet-bounces@lists.utsouthwestern.edu > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and > I was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of > placing both liver halves into the tissue processor. The liver I > intended for OCT embedding is now hard as wax. Is there any way to > deparaffinize processed organs and may I embed them in OCT for proper > cryosectioning? I imagine that the liver would get dehydrated, I would > get crappy sections, and Oil Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu 'Histonet@lists.utsouthwestern.edu');> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 14 Nov 2012 11:07:35 +0100 From: "Jean-Philippe Berteau" Subject: [Histonet] Technovit 9100 new fails to polymerize To: Message-ID: <001001cdc24f$deaa7390$9bff5ab0$@tu-harburg.de> Content-Type: text/plain; charset="us-ascii" Hello, I got the same problem, how did you solve it ? Best regards Jean-Philippe ------------------------------ Message: 12 Date: Wed, 14 Nov 2012 11:13:58 +0100 From: "Jean-Philippe Berteau" Subject: [Histonet] Technovit 9100 new fails to polymerize To: Message-ID: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de> Content-Type: text/plain; charset="us-ascii" Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ------------------------------ Message: 13 Date: Wed, 14 Nov 2012 05:58:25 -0500 From: Tom McNemar Subject: [Histonet] RE: staffing numbers To: "'Hutton, Allison'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Allison, We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep. We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 14 Date: Wed, 14 Nov 2012 06:43:56 -0600 From: "Marsh, Nannette" Subject: RE: [Histonet] Technovit 9100 new fails to polymerize To: "'Jean-Philippe Berteau'" , "histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9078ED7C148@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau Sent: Wednesday, November 14, 2012 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 new fails to polymerize Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 14 Nov 2012 15:26:26 +0000 From: CHRISTIE GOWAN Subject: [Histonet] TBS Slide Printer To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks. Christie ------------------------------ Message: 16 Date: Wed, 14 Nov 2012 09:43:00 -0600 From: Vanessa Perez Subject: [Histonet] RE: liftin tissue To: Lee Loss , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use Mount quick media, which comes with a procedure for section transfer.... Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, November 13, 2012 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] liftin tissue I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 14 Nov 2012 07:43:56 -0800 From: Carla M Conway Subject: [Histonet] Zinc formalin as primary fixative for TEM To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello everyone, A colleague has tissues which are fixed in zinc formalin and wants to examine them by transmission electron microscopy. Any thoughts regarding the use of this fixative for EM? Thanks in advance, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov ------------------------------ Message: 18 Date: Wed, 14 Nov 2012 09:50:34 -0600 From: Jaclyn Pitts Subject: [Histonet] H&E Question To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ------------------------------ Message: 19 Date: Wed, 14 Nov 2012 15:52:11 +0000 From: "Craven Peter (NHS HIGHLAND)" Subject: RE: [Histonet] H&E Question To: Jaclyn Pitts , "histonet@lists.utsouthwestern.edu" Message-ID: <20121114155324.DDD98448D6F@nhs-pd1e-esg101.ad1.nhs.net> Content-Type: text/plain; charset="us-ascii" Jaclyn it sounds daft but check the pH of your Eosin we found this can be strongly acidic. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jaclyn@gmail.com] Sent: 14 November 2012 03:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** ------------------------------ Message: 20 Date: Wed, 14 Nov 2012 16:02:57 +0000 From: "Goins, Tresa" Subject: RE: [Histonet] H&E Question To: Jaclyn Pitts , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 14 Nov 2012 11:11:34 -0500 From: "Pam Barker" Subject: [Histonet] Histology Supervisor Needed in Atlanta. Can you help? To: "Histonet" Message-ID: <046c01cdc282$b7f69520$27e3bf60$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I hope everybody is having a great day. I wanted to drop you a quick line to ask for help because I am currently recruiting for one of my best clients located in Atlanta, GA. We are looking for an experienced Histology Supervisor. We are looking for someone who is ASCP certified with at least 3 years of histology and 2 years of experience supervising a histology lab. They are offering a great salary, terrific benefits, the stability of a large company and a great group of people to work with. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at relia1@earthlink.net or toll free at 866-607-3542. Remember if I hire someone you refer you will receive a referral bonus! Thank you, Pam - 866-607-3542 (866-60RELIA) - Toll Free Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 22 Date: Wed, 14 Nov 2012 08:20:00 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] H&E Question To: Jaclyn Pitts , "histonet@lists.utsouthwestern.edu" Message-ID: <1352910000.32673.YahooMailNeo@web163101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I never heard of "Azer Scientific hematoxylin" but if you can leave the sections in it during 12 minutes and it is still "weak" for your pathologist, it seems that it is a "progressive" hematoxylin, of the Mayer type. Progressive hematoxylins are somewhat tricky and have to be very fresh to work well. I have always used and preferred "regressive" hematoxylins of the Harris' type. With them you are in control of the staining when you differentiate them up to the strength?proffered by your pathologist. My advise, since it seems that you cannot satisfy your pathologist's demands,is to switch to a Harris type hematoxylin and stop fiddling with the hematoxylin you are using. I always used and preferred Harris by Richard Alan. Switch and your problems will be over. Ren? J. ________________________________ From: Jaclyn Pitts To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 10:50 AM Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5? min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 108, Issue 18 ***************************************** From m.kap.1 <@t> erasmusmc.nl Wed Nov 14 12:15:21 2012 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Wed Nov 14 12:15:32 2012 Subject: [Histonet] SPIDIA Prostate Tumor Ring Trial Message-ID: <7CD0BD0DBF66F94F96E9A75A96D7F8EFE3DC62@EXCH-RX02.erasmusmc.nl> Hi Histonetters, I need some prostate experts to evaluate some virtual slides for a world wide morphology ring trial. We started the ring trial already in August, but a few of the participants don't respond. If you know anyone who would be interested, please ask them to contact me (m.kap.1@erasmusmc.nl) for more info. Thanx! Marcel From Albert.Santiago <@t> uphs.upenn.edu Wed Nov 14 12:47:36 2012 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Nov 14 12:47:45 2012 Subject: [Histonet] FW: holes in tissue sections Message-ID: <63C408E65F57294790C8CFA2150A6475984BE7@uphmasphi013.UPHS.PENNHEALTH.PRV> From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice on how to resolve this problem: We are experiencing "holes" in tissue sections. The holes appear mainly in the epidermis but sometimes in the dermis. I've tried a few different things but still haven't resolved. If someone has experienced or seen this please help. Any and all feedback will be appreciated. I have some sample photos for your review, if anyone would like to see what I'm talking about. Please let me know and I'll email them to you. Thank you, Albert The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From LPaveli1 <@t> hurleymc.com Wed Nov 14 12:59:55 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Nov 14 13:00:07 2012 Subject: [Histonet] RE: holes in tissue sections In-Reply-To: <63C408E65F57294790C8CFA2150A6475984BE7@uphmasphi013.UPHS.PENNHEALTH.PRV> References: <63C408E65F57294790C8CFA2150A6475984BE7@uphmasphi013.UPHS.PENNHEALTH.PRV> Message-ID: <89F4666A496DC949A819ECC40E11C867BF5694F9@EXCHANGEMB1.hmc.hurleymc.com> Hi Albert, The first thing that comes to mind to me is aggressive "facing" or rough cutting of the block before taking your final good section. To solve this, after facing the block, (usually at 20+ microns), I go down to my desired slide micron (4) and finish facing at the lower micron for 5-10 more times. This will remove the holes caused by the high microns. Hope this helps, Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Santiago, Albert [Albert.Santiago@uphs.upenn.edu] Sent: Wednesday, November 14, 2012 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: holes in tissue sections From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice on how to resolve this problem: We are experiencing "holes" in tissue sections. The holes appear mainly in the epidermis but sometimes in the dermis. I've tried a few different things but still haven't resolved. If someone has experienced or seen this please help. Any and all feedback will be appreciated. I have some sample photos for your review, if anyone would like to see what I'm talking about. Please let me know and I'll email them to you. Thank you, Albert The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathology.histology <@t> gmail.com Wed Nov 14 13:01:40 2012 From: pathology.histology <@t> gmail.com (path lab) Date: Wed Nov 14 13:01:47 2012 Subject: [Histonet] preserving hydrogel in tissue sections Message-ID: We were asked to perform histology on goat knee joints (fixed in 10%NBF) to demonstrate a polymeric material - hydrogel. Could someone please share their procedure or direct me to someone who could help me? I couldn't find much information using PEG and are open to using other methods. We would like to preserve the hydrogel for microscopic evaluation if at all possible. Thanks in advance. From Fawn.Bomar <@t> HalifaxRegional.com Wed Nov 14 13:22:49 2012 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Nov 14 13:22:59 2012 Subject: [Histonet] Control Block Log Sheets Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B93EA2BC@EXCH-2K10.hrhs.com> Does anyone have a template they would be willing to share on how to keep track of control blocks? Do you all use a type of log sheet to keep track, do you all number them a certain way to keep track? Thank you Fawn From PMonfils <@t> Lifespan.org Wed Nov 14 13:40:02 2012 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Nov 14 13:40:07 2012 Subject: [Histonet] Gomori Trichrome and nemaline bodies? Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835D5C@LSRIEXCH1.lsmaster.lifespan.org> Hello All, I work in a core facility providing laboratory services to researchers. I had a request to cut some frozen sections on muscle tissue, and stain them with Gomori Trichrome, a technique we use routinely. I did the stain, checked them microscopically andconfirmed that the collagen fibers were nicely stained. But the researcher came back and said that the slides did not show what she was looking for, which was nemaline bodies, rod-shaped structures indicative of a disease called nemaline myopathy. I had never heard of this but went online and found several articles on the disease, a couple of which showed images of nemaline bodies stained with a "modified Gomori Trichrome" procedure. But no indications of what the modifications were. Is anyone familiar with the staining of these bodies, either using a modified Gomori trichrome, or some other method? Thanks. Paul Monfils From LPaveli1 <@t> hurleymc.com Wed Nov 14 13:46:11 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Nov 14 13:46:33 2012 Subject: [Histonet] RE: Gomori Trichrome and nemaline bodies? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835D5C@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835D5C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56954B@EXCHANGEMB1.hmc.hurleymc.com> I have attached our Modified Gomori's Trichrome procedure. Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Monfils, Paul [PMonfils@Lifespan.org] Sent: Wednesday, November 14, 2012 2:40 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome and nemaline bodies? Hello All, I work in a core facility providing laboratory services to researchers. I had a request to cut some frozen sections on muscle tissue, and stain them with Gomori Trichrome, a technique we use routinely. I did the stain, checked them microscopically andconfirmed that the collagen fibers were nicely stained. But the researcher came back and said that the slides did not show what she was looking for, which was nemaline bodies, rod-shaped structures indicative of a disease called nemaline myopathy. I had never heard of this but went online and found several articles on the disease, a couple of which showed images of nemaline bodies stained with a "modified Gomori Trichrome" procedure. But no indications of what the modifications were. Is anyone familiar with the staining of these bodies, either using a modified Gomori trichrome, or some other method? Thanks. Paul Monfils _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Nov 14 14:04:12 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 14 14:04:17 2012 Subject: [Histonet] FW: holes in tissue sections In-Reply-To: References: <63C408E65F57294790C8CFA2150A6475984BE7@uphmasphi013.UPHS.PENNHEALTH.PRV>, , <63C408E65F57294790C8CFA2150A6475984BF1@uphmasphi013.UPHS.PENNHEALTH.PRV>, Message-ID: From: joelleweaver@hotmail.com To: albert.santiago@uphs.upenn.edu Subject: RE: [Histonet] FW: holes in tissue sections Date: Wed, 14 Nov 2012 19:47:46 +0000 Thank you for the pictures. Yes, this appears to be a physical tearing. I agree with the other postee on the histonet that this is more likely due to microtomy technique. Specifically, due to "rough" facing, and then taking a section too early after returing to normal section thickness with an automated microtome or too rapid sectioning ( stroke speed)on a manual for the ribbon from which the final section is selected. With some skins, if they are somewhat dense, and (the tissue can sometimes even appear white in color if there are dehydration issues), I find that if you face a little more gingerly and then soak them in some very cold ice water and not just hardened ice, then enough moisture transfers to help make it pliable. I sometimes use the warm water bath and then transfer them back to the cold to get a bit more moisture. This always seems to work pretty well and produces a smoother section without tearing. Most times you can see this artifact on the waterbath if you are watching for it. I never take the first ribbons or sections that appear first on the knife blade with any sections due to this artifact and also the greater thickness of these sections. Alternatively, tearing can occur when the knife blade is not shifted after facing and debris of paraffin and excess tissue dull and remain on the blade( or behind the blade) from facing and then cause tears in sections from the extra drag on the blade edge. Just use one part of the blade only for facing and move to a fresh blade section only for final sections and watch carefully for dragging and tearing as the knift dulls on the water bath. Maybe give these technique adjustments a try first, and I hope that it will simply and quickly solve your issues. Joelle Weaver MAOM, HTL (ASCP) QIHC Subject: RE: [Histonet] FW: holes in tissue sections Date: Wed, 14 Nov 2012 14:28:17 -0500 From: Albert.Santiago@uphs.upenn.edu To: joelleweaver@hotmail.com Hi Joelle, I have attached the photos per your request for your review. Thank you very much for taking the time to look into this. Thank you From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Wednesday, November 14, 2012 2:04 PM To: Santiago, Albert Subject: RE: [Histonet] FW: holes in tissue sections Yes images would be helpful. Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 14 Nov 2012 13:47:36 -0500 > From: Albert.Santiago@uphs.upenn.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FW: holes in tissue sections > > > > > > From: Santiago, Albert > Sent: Wednesday, November 14, 2012 1:35 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: holes in tissue sections > > > > Hello fellow histonetters, We are a derm lab and we are having slide > quality issues, I am hoping someone can provide me with some advice on > how to resolve this problem: > > We are experiencing "holes" in tissue sections. The holes appear mainly > in the epidermis but sometimes in the dermis. I've tried a few different > things but still haven't resolved. If someone has experienced or seen > this please help. Any and all feedback will be appreciated. > > I have some sample photos for your review, if anyone would like to see > what I'm talking about. Please let me know and I'll email them to you. > > > > Thank you, > > Albert > > > > > > > > > > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From AJohnson <@t> aipathology.com Wed Nov 14 14:10:55 2012 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Wed Nov 14 14:11:01 2012 Subject: [Histonet] test Message-ID: <704247D5A09D004C9E6B115138D1703A5492E1@hpserv001.aipathology.local> test From TGoins <@t> mt.gov Wed Nov 14 16:00:04 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Nov 14 16:00:19 2012 Subject: [Histonet] RE: Control Block Log Sheets In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B93EA2BC@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B93EA2BC@EXCH-2K10.hrhs.com> Message-ID: We write the accession number on the control block itself and transfer that ID to the slide label and to the data entry system when the control block is used. This creates a permanent record while eliminating the need for a log that continually needs updating. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, November 14, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Block Log Sheets Does anyone have a template they would be willing to share on how to keep track of control blocks? Do you all use a type of log sheet to keep track, do you all number them a certain way to keep track? Thank you Fawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM <@t> mcclainlab.com Thu Nov 15 04:24:48 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Thu Nov 15 04:25:05 2012 Subject: [Histonet] RE: Histonet Digest, Vol 108, Issue 19 H&E staining Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF3053DBA3@ML1.McClainLabs.local> Eosin tissue affinity vs hema is far different with staining times measured in seconds for Eosin vs minutes for Hema. Overstained Dark eosin makes H appear too light by contrast. If one considers an analogy of a balance or seesaw with H on one end and E on the other, your solution is obvious. When H balances E staining appears optimally dark. Subtracting eosin to properly balance hema makes whole stain appear more vibrant. A vivid triple eosin- Treosin is available commercially from Statlab, (satisfied customer- no financial ties) Eosin Y and Orange G and acid fuchsin offer differential staining of collagen, fibrin, muscle, cornified cells, especially good for eosinophils and RBCs. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From DianaRip1 <@t> aol.com Thu Nov 15 07:21:32 2012 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Nov 15 07:21:45 2012 Subject: [Histonet] Re: Histonet Digest, Vol 108, Issue 19 Message-ID: One technique that has worked well for me is to cut the portion of coverslip off of the half of the slide over the section you need to stain. In a message dated 11/14/2012 10:00:31 A.M. Pacific Standard Time, histonet-request@lists.utsouthwestern.edu writes: LLoss@dermwisconsin.com From Rcartun <@t> harthosp.org Thu Nov 15 10:42:53 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Nov 15 10:43:04 2012 Subject: [Histonet] IHC for ZEB1 Message-ID: <50A4D53D.7400.0077.1@harthosp.org> Is anyone doing IHC for ZEB1 on formalin-fixed, paraffin-embedded tissue? If so, whose antibody are you using? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax From BMolinari <@t> texasheart.org Thu Nov 15 11:45:22 2012 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Thu Nov 15 11:45:51 2012 Subject: [Histonet] Verhoeff's with a green counterstain Message-ID: Hi, I have not heard of this stain before, but saw mention of it in a paper. I could not find a protocol. Could someone tell me more about it? Thanks! Betsy Molinart HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houaton, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. From oneilb <@t> wvuhealthcare.com Thu Nov 15 13:07:03 2012 From: oneilb <@t> wvuhealthcare.com (Oneil, Beth Ann) Date: Thu Nov 15 13:07:18 2012 Subject: [Histonet] Auramine O Rhodamine B for acid fast bacteria Message-ID: <3CEB8EBCF9C7A648B9694B5696462A7103B3D4@NT-EXMB1.wvuh.wvuhs.com> We are bringing the Auramine O Rhodamine B stain for Acid Fast Bacteria in house very soon. My question is can you store the slides indefinitely? With our immunofluororesce testing on kidney biopsies, the slides are not stable. I have not seen any literature in regards to the stability of the Auramine-Rhodamine stain. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals 304-293-7629 (office) 304-293-6014 (lab) From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 15 13:20:04 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Nov 15 13:20:17 2012 Subject: [Histonet] implatna and hardware retention Message-ID: <761E2B5697F795489C8710BCC72141FF02CF9E@ex07.net.ucsf.edu> Hi all, how long do you keep breast explants and ortho hardware? Currently we are keeping them indefinitely and 5 years respectively. Any references for standards on this? Thanks! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From rjbuesa <@t> yahoo.com Thu Nov 15 13:30:24 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 15 13:30:28 2012 Subject: [Histonet] Auramine O Rhodamine B for acid fast bacteria In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A7103B3D4@NT-EXMB1.wvuh.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A7103B3D4@NT-EXMB1.wvuh.wvuhs.com> Message-ID: <1353007824.43627.YahooMailNeo@web163104.mail.bf1.yahoo.com> Auramine O is designed to view the slides with fluorescent light and as in any such procedure the slides are not stable and cannot be stored indefinitely. On the other hand it is a very simple procedure and if your microscope objectives are?good and the light source adequate it can render better results than Ziehl-Nielsen because it can demonstrate low level infections (only few organisms) better. Ren? J. ________________________________ From: "Oneil, Beth Ann" To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, November 15, 2012 2:07 PM Subject: [Histonet] Auramine O Rhodamine B for acid fast bacteria We are bringing the Auramine O Rhodamine B stain for Acid Fast Bacteria in house very soon.? My question is can you store the slides indefinitely?? With our? immunofluororesce testing on kidney biopsies, the slides are not stable.? I have not seen any literature in regards to the stability of the Auramine-Rhodamine stain. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals 304-293-7629 (office) 304-293-6014 (lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jamie.Howard <@t> uky.edu Thu Nov 15 13:52:30 2012 From: Jamie.Howard <@t> uky.edu (Howard, Jamie C) Date: Thu Nov 15 13:52:34 2012 Subject: [Histonet] Tissue Tek VIP 5 processor Message-ID: <90C4820E7D729644ADF9E3FC4518DFA408148A@ex10mb01.ad.uky.edu> We have had our Tissue Tek VIP 5 floor model processor for five years. Here is a little background: We are an animal diagnostic facility, there is no formalin in the processing cycle, and we perform weekly cleaning/maintenance. The retort chamber is wiped down every day after the processing and cleaning run. However, the retort chamber has recently had a green discoloration form on the sides and bottom and I'm not sure what would be causing it. Any ideas? Thanks, Jamie From rjbuesa <@t> yahoo.com Thu Nov 15 13:59:31 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 15 13:59:35 2012 Subject: [Histonet] Tissue Tek VIP 5 processor In-Reply-To: <90C4820E7D729644ADF9E3FC4518DFA408148A@ex10mb01.ad.uky.edu> References: <90C4820E7D729644ADF9E3FC4518DFA408148A@ex10mb01.ad.uky.edu> Message-ID: <1353009571.12692.YahooMailNeo@web163103.mail.bf1.yahoo.com> If by green discoloration you mean that it seems as if the metal is greenish? If that is the case maybe so much scrubbing may have removed part of the nickel platting of the retort and a copper shim is becoming evident (I think). Ren? J. ________________________________ From: "Howard, Jamie C" To: "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, November 15, 2012 2:52 PM Subject: [Histonet] Tissue Tek VIP 5 processor We have had our Tissue Tek VIP 5 floor model processor for five years. Here is a little background: We are an animal diagnostic facility, there is no formalin in the processing cycle, and we perform weekly cleaning/maintenance. The retort chamber is wiped down every day after the processing and cleaning run. However, the retort chamber has recently had a green discoloration form on the sides and bottom and I'm not sure what would be causing it. Any ideas? Thanks, Jamie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Nov 15 13:59:39 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Nov 15 13:59:43 2012 Subject: [Histonet] Tissue Tek VIP 5 processor In-Reply-To: <90C4820E7D729644ADF9E3FC4518DFA408148A@ex10mb01.ad.uky.edu> References: <90C4820E7D729644ADF9E3FC4518DFA408148A@ex10mb01.ad.uky.edu> Message-ID: Are you marking your tissues with ink/dyes? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Nov 15, 2012 at 1:52 PM, Howard, Jamie C wrote: > We have had our Tissue Tek VIP 5 floor model processor for five years. > Here is a little background: We are an animal diagnostic facility, there is > no formalin in the processing cycle, and we perform weekly > cleaning/maintenance. The retort chamber is wiped down every day after the > processing and cleaning run. However, the retort chamber has recently had a > green discoloration form on the sides and bottom and I'm not sure what > would be causing it. Any ideas? > > Thanks, > > Jamie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jqb7 <@t> cdc.gov Fri Nov 16 07:40:23 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Nov 16 08:12:37 2012 Subject: [Histonet] Sugar cane Message-ID: Morning everyone, Does anyone have a protocol for the processing of sugar cane they can share? Thanks much! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartlett@cdc.hhs.gov From joewalker <@t> rrmc.org Fri Nov 16 08:54:42 2012 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Nov 16 08:54:46 2012 Subject: [Histonet] RE: Sugar cane In-Reply-To: References: Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1794A515@RRMBX03.rrmc.local> Into Rum? That might be illegal without a permit. :o) I apologize in advance but I couldn't resist a little attempt at humor on this one. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Friday, November 16, 2012 8:40 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sugar cane Morning everyone, Does anyone have a protocol for the processing of sugar cane they can share? Thanks much! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From rjbuesa <@t> yahoo.com Fri Nov 16 09:47:25 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 16 09:47:36 2012 Subject: [Histonet] Sugar cane In-Reply-To: References: Message-ID: <1353080845.52834.YahooMailNeo@web163105.mail.bf1.yahoo.com> The only thing difficult about sugar cane (I imagine the stems) is that it contains silicon spikes that can be very difficult to section because those fibers can affect the edge of the sectioning blades. Other than that you can use any method for plant tissues. Ren? J. ________________________________ From: "Bartlett, Jeanine (CDC/OID/NCEZID)" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, November 16, 2012 8:40 AM Subject: [Histonet] Sugar cane Morning everyone, Does anyone have a? protocol for the processing of sugar cane they can share? Thanks much! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Fri Nov 16 09:54:55 2012 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Nov 16 09:55:03 2012 Subject: [Histonet] procedure for destaining a cytology slide followed by restaining with a special stain, such as PAS? Message-ID: Does anyone have a procedure for restaining a cytology slide? We experienced a loss of cells on an FNA aspirate of a lymph node, which had been originally stained with Diff Qwik. The procedure we used was as follows: 1) Destain using 1% Acid Alcohol (1 ml. concentrated HCL added to 99 ml. 70% alcohol) for approx. 30-60 seconds. 2) Restain on automated stainer (Ventana) with PAS kit. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From amber.mckenzie <@t> gastrodocs.net Fri Nov 16 10:02:02 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Nov 16 09:59:06 2012 Subject: [Histonet] High Complexity testing In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC7B5CC9AB@JERRY.Gia.com> Does anyone know where to find the High complexity testing requirements for CAP? We're about to apply for CAP accreditation and want to make sure the person working in our lab grossing has the requirements. Thanks! From SteveM <@t> mcclainlab.com Fri Nov 16 10:19:47 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Fri Nov 16 10:20:07 2012 Subject: [Histonet] holes in derm tissue sections Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF3053DDEF@ML1.McClainLabs.local> Generalized Holes commonly present with one of two scenarios. Scenario One-a Histotech may be sloppy about allowing sections to dry thoroughly before warming or baking. Scenario Two is generalized, showing up with slides from every Histotech, and representing unstabilized foci where paraffin did not penetrate, eg a processing failure. Label it as a QI event, call it "catastrophic tissue loss". With that mindset, Examine slides microscopically. Establish baseline: Measure one whole day by examining every slide and counting how many have holes or unstable collagen. Secondarily Pay attention to/ record tissue size. Simple Remedies-Three processing areas to examine. Change your processing alcohols, using NEW, not recycled. Change your xylenes, NEW, not recycled. Rotate paraffins and Lengthen time in last clean paraffin by 25%. Measure again. Examine the remaining failures. "catastrophic tissue loss" may sound a bit harsh, but I have special fondness for derm and hold them to a higher standard. Derm labs which cannot reliably find satellite metastasis of melanoma are XXX007. So label it for what it is- "catastrophic tissue loss". Then put your foot on the gas and fix it urgently, and follow-up those remaining failures. Even if long-term you change your processing program or process according to size or take formalin off your processors. or more drastically, change behavior among grossers to assure proper fixation and slicing of excision specimens. #4 POST-FIX large blocks, those >10mm after gross slicing to proper thickness, place cassettes in fresh formalin for several hours. Change formalin on grossing bench daily. If you post-fix on the tissue processor, change or rotate formalin daily. Dirty formalin smells bad but does not fix well. The sniff test is inaccurate for determining formalin activity. #5 Spreading out cassettes in processing baskets allows for more diffusion. Not more than two high. Stay with it Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 16 12:11:34 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Nov 16 12:11:48 2012 Subject: [Histonet] Nerve teasing videos? Message-ID: <761E2B5697F795489C8710BCC72141FF02D174@ex07.net.ucsf.edu> Hi all, does anyone know of instructional videos for nerve teasing methods? I tried Youtube but didn't find anything... Thanks in advance! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From histonetic <@t> inbox.com Fri Nov 16 12:18:43 2012 From: histonetic <@t> inbox.com (H & E *) Date: Fri Nov 16 12:18:45 2012 Subject: [Histonet] 88305TC starting to hit the fan... Message-ID: <1FAE3C79AFB.00000430histonetic@inbox.com> The news about the decrease of the 88305TC medicaire reimbursement fee was just announced at my lab finally. This of course struck fear in the entire lab workforce because it's clear some major belt-tightening will have to be done, and it's most likely to hurt everybody. Costs and probably hours will have to be reduced it seems clear. Isn't the finaly figure for this cut (%) supposed to be announced today? I have heard anywhere from 20 to 52% is possible? This will give a final measure as to how many belt loops we'll have to cinch-in... Anybody else out there starting to "feel the pain" in their labs yet? ____________________________________________________________ FREE ONLINE PHOTOSHARING - Share your photos online with your friends and family! Visit http://www.inbox.com/photosharing to find out more! From lguernsey <@t> ucsd.edu Fri Nov 16 13:41:57 2012 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Fri Nov 16 13:42:43 2012 Subject: [Histonet] Nerve teasing videos? In-Reply-To: <761E2B5697F795489C8710BCC72141FF02D174@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF02D174@ex07.net.ucsf.edu> Message-ID: If the videos exist, I'm very interested in this as well. Thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu On Fri, Nov 16, 2012 at 10:11 AM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > Hi all, does anyone know of instructional videos for nerve teasing > methods? I tried Youtube but didn't find anything... > > Thanks in advance! > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94143 > > (415) 353-1266 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PAMarcum <@t> uams.edu Fri Nov 16 15:07:43 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Nov 16 15:08:10 2012 Subject: [Histonet] University of Arkansas for Medical Sciences Has Two HT or HTL Positions Open Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA328A56E5DB@Mail2Node2.ad.uams.edu> We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming. Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.) Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO) Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From pruegg <@t> ihctech.net Sun Nov 18 08:45:42 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 18 08:45:39 2012 Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had tobe in OCT instead)! In-Reply-To: References: Message-ID: <927F7AB460684129B089F28CE49FCA90@DESKTOP3> Yea if you were looking for fat that will be lost with paraffin processing, so the oil red o for fat will not work. I would not see any point in trying to depara and then snap freeze, but if I did want to do that since the tissue is formalin fixed I would depara, fix some more in formalin and then infiltrate the tissue in 30% sucrose overnight before snap freezing, if you do not do that fix tissue will not section well frozen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, November 13, 2012 10:15 AM To: z o n k e d Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin (had tobe in OCT instead)! It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d To: "histonet@lists.utsouthwestern.edu" Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-bounces@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 18 08:50:11 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 18 08:50:10 2012 Subject: [Histonet] Microtome knives In-Reply-To: <1352627080.42134.YahooMailNeo@web171201.mail.ir2.yahoo.com> References: <1352627080.42134.YahooMailNeo@web171201.mail.ir2.yahoo.com> Message-ID: <781F33CD0401495DB2AAE3418FBA9F18@DESKTOP3> There are knife sharpening services Sturkey and Dorn and Hart are two that come to mind. You can also find refurbished disposable blade holders and buy disposable blades, the blade holder you get will determine if you use low profile or high profile blades. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Massimo Sent: Sunday, November 11, 2012 2:45 AM To: Jon Krupp; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome knives I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Jon Krupp A: Histonet@lists.utsouthwestern.edu Inviato: Venerd? 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not? histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA? 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 18 08:53:56 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 18 08:53:52 2012 Subject: [Histonet] decal affecting IHC In-Reply-To: <14E2C6176416974295479C64A11CB9AE01640B28DBBB@SBS2K8.premierlab.local> References: <1352483229.63600.YahooMailNeo@web163105.mail.bf1.yahoo.com> <14E2C6176416974295479C64A11CB9AE01640B28DBBB@SBS2K8.premierlab.local> Message-ID: I agree with Liz and use 5-10% formic acid for decal for IHC work, on the other hand if you are trying to demonstrate iron deposits or some enzyme histochemical stains such as TRAP or Alk.phos you may need to use EDTA. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, November 09, 2012 11:00 AM To: 'Rene J Buesa'; Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] decal affecting IHC Diana I agree with Jack, formic acid is the way to go in my opinion, we use 10% formic acid routinely but have pushed it up to 20% on occasion when time was an issue. HCL can work if its controlled really well but you don't have much wiggle room, you can over decal quite quickly, formic acid is a bit more forgiving. As for Rene's comment we have seen the exact opposite with respects to the EDTA decal part. Granted we do not run a lot of IHC on EDTA decaled samples but on several occasions and with several antibodies we were able to obtain good IHC staining in formic acid decaled samples but those antibodies did not work well in EDTA decaled samples. These were not direct comparisons of the decalcification method on the same study, but on samples from different studies so other factors could have affected the results. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, November 09, 2012 10:47 AM To: Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] decal affecting IHC IHC reactions can be affected by decalcification especially if: 1- the tissue is not perfectly fixed before going into decalcification 2- decalcification is done at temperature above room temp. 3- decal solution is too acid, specially made with inorganic acids. If at all possible decalcification should be done with a chelating agent, like EDTA Ren? J. ________________________________ From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, November 9, 2012 12:34 PM Subject: [Histonet] decal affecting IHC Does decalcifying tissue (Calex II) affect the antibody reaction for IHC in bony tissue? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sugartop2000 <@t> gmail.com Sun Nov 18 15:19:52 2012 From: sugartop2000 <@t> gmail.com (Sirena Hudgins) Date: Sun Nov 18 15:19:58 2012 Subject: [Histonet] remove me from email list Message-ID: please remove me from your email list thanks, Sirena From rob <@t> foliobio.com Mon Nov 19 10:03:50 2012 From: rob <@t> foliobio.com (Rob Day) Date: Mon Nov 19 10:03:57 2012 Subject: [Histonet] Need better results from older archival slides? Message-ID: <3B0D7990-AF90-4ED2-99DE-0AF99BB1D4A7@foliobio.com> Hello, Having problems getting good Immuno staining and in-situ hybridization results with older slides? I am writing to see if histonet can suggest any scientists who may be interested in helping us with a study related to a "slide rejuvenation" process we are developing. We think we have found a way to significantly improve the antigenicity of proteins in older archival FFPE preserved slides. Initial tests seem to indicate that our process can allow slides of 5-10 years age, possibly older, to respond to IHC analysis as if they were cut that day. The process may have additional uses and benefits too, including better preservation of cut sections, enhanced ISH results, stabilization of labile proteins and standardization / optimization of IHC staining protocols. We are excited about this development, but we are seeking some independent labs to help us verify our findings. Please feel free to call me to discuss, or pass on this email to any investigators you may know of who are exploring ways to make better use of older archival slides. Folio Biosciences is a provider of biosamples and a commercial research lab based in Columbus Ohio. See: www.foliobio.com. Regards, Rob Day. Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. From rjbuesa <@t> yahoo.com Mon Nov 19 10:16:15 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 19 10:16:20 2012 Subject: [Histonet] Need better results from older archival slides? In-Reply-To: <3B0D7990-AF90-4ED2-99DE-0AF99BB1D4A7@foliobio.com> References: <3B0D7990-AF90-4ED2-99DE-0AF99BB1D4A7@foliobio.com> Message-ID: <1353341775.11990.YahooMailNeo@web163102.mail.bf1.yahoo.com> Rob: If you are really seeking for a confirming study, publish your data and probably there will be a confirmation study. ? I went to the website you indicated and it seems to me that your original posting is just a "subtle and delicate" advertisement. ? Just my opinion otherwise, why don't you tell us all what is that you do to obtain your rejuvenating results? Ren? J. ________________________________ From: Rob Day To: histonet@lists.utsouthwestern.edu Sent: Monday, November 19, 2012 11:03 AM Subject: [Histonet] Need better results from older archival slides? Hello, Having problems getting good Immuno staining and in-situ hybridization results with older slides? I am writing to see if histonet can suggest any scientists who may be interested in helping us with a study related to a? "slide rejuvenation" process we are developing. We think? we have found a way to significantly improve the antigenicity of proteins in older archival FFPE preserved slides. Initial tests seem to indicate that our process can allow slides of 5-10 years age, possibly older, to respond to IHC analysis as if they were cut that day. The process may have additional uses and benefits too, including better preservation of cut sections, enhanced ISH results, stabilization of labile proteins and standardization / optimization of IHC staining protocols. We are excited about this development, but we are seeking some independent labs to help us verify our findings. Please feel free to call me to discuss, or pass on this email to any investigators you may know of who are exploring ways to make better use of older archival slides. Folio Biosciences is a provider of biosamples and a commercial research lab based in Columbus Ohio. See: http://www.foliobio.com/. Regards, Rob Day. Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 |? Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Mon Nov 19 10:22:02 2012 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Nov 19 10:22:24 2012 Subject: [Histonet] Need better results from older archival slides? In-Reply-To: <1353341775.11990.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <3B0D7990-AF90-4ED2-99DE-0AF99BB1D4A7@foliobio.com> <1353341775.11990.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: Agreed! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, November 19, 2012 11:16 AM To: Rob Day; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need better results from older archival slides? Rob: If you are really seeking for a confirming study, publish your data and probably there will be a confirmation study. ? I went to the website you indicated and it seems to me that your original posting is just a "subtle and delicate" advertisement. ? Just my opinion otherwise, why don't you tell us all what is that you do to obtain your rejuvenating results? Ren? J. ________________________________ From: Rob Day To: histonet@lists.utsouthwestern.edu Sent: Monday, November 19, 2012 11:03 AM Subject: [Histonet] Need better results from older archival slides? Hello, Having problems getting good Immuno staining and in-situ hybridization results with older slides? I am writing to see if histonet can suggest any scientists who may be interested in helping us with a study related to a? "slide rejuvenation" process we are developing. We think? we have found a way to significantly improve the antigenicity of proteins in older archival FFPE preserved slides. Initial tests seem to indicate that our process can allow slides of 5-10 years age, possibly older, to respond to IHC analysis as if they were cut that day. The process may have additional uses and benefits too, including better preservation of cut sections, enhanced ISH results, stabilization of labile proteins and standardization / optimization of IHC staining protocols. We are excited about this development, but we are seeking some independent labs to help us verify our findings. Please feel free to call me to discuss, or pass on this email to any investigators you may know of who are exploring ways to make better use of older archival slides. Folio Biosciences is a provider of biosamples and a commercial research lab based in Columbus Ohio. See: http://www.foliobio.com/. Regards, Rob Day. Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 |? Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kntapolias <@t> jgh.mcgill.ca Mon Nov 19 15:04:01 2012 From: kntapolias <@t> jgh.mcgill.ca (kntapolias@jgh.mcgill.ca) Date: Mon Nov 19 15:17:22 2012 Subject: [Histonet] AUTO: Katerina Ntapolias is out of the office. (returning 2012-11-21) Message-ID: I am out of the office until 2012-11-21. I will respond to your message when I return. Katerina Ntapolias Note: This is an automated response to your message "Histonet Digest, Vol 108, Issue 24" sent on 11/19/2012 2:39:13 PM. This is the only notification you will receive while this person is away. From twebster <@t> CRH.org Mon Nov 19 15:25:33 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Mon Nov 19 15:25:39 2012 Subject: [Histonet] 88305TC starting to hit the fan... Message-ID: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From jaylundgren <@t> gmail.com Mon Nov 19 15:56:26 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Nov 19 15:56:30 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> Message-ID: Because there is a huge shortage of primary care docs as it is, which will only get worse when millions of uninsured people suddenly have insurance. So I guess they are trying to entice more med students into primary care. There is a push to train more physician's assistants too As it is, the top medical students want to go into something like radiology or dermatology so they can work banker's hours and make 5x as much money as a PCHP, and who can blame them? I friend of mine who is a radiology tech saw a locum radiologist's pay stub left on the fax machine (by accident), and said radiologist makes $280./hr. Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Nov 19, 2012 at 3:25 PM, Webster, Thomas S. wrote: > CAP had a webinar last week about the cut. These are some very scary > times. For some reason the government has decided to shift wealth from > specialists to family practice. I am becoming more angry with the > affordable care act everyday. > > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BGapinski <@t> pathgroup.com Mon Nov 19 16:15:02 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Mon Nov 19 16:15:19 2012 Subject: [Histonet] 88305 Message-ID: I am in the middle of the biggest cost study because we need to reevaluate the cost of our work. We have no expectation of keeping any derm clients after the change in the 88305. They will go elsewhere to get a slightly better price than here in California. This is depressing on many levels. 1. Lay-offs 2. Inferior quality (we are VERY proud of our work) 3. TAT nightmares. 4. Where does that leave our true client ( THE PATIENT)? Furthermore I may be out of a job (last 35 years) and the pathologists may farm out our surgical work too. How does this impact your Histology laboratory? Respectfully, Bruce Gapinski Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From pathlocums <@t> gmail.com Mon Nov 19 17:46:03 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Nov 19 17:46:10 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> Message-ID: <-3423156224104217588@unknownmsgid> While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Mon Nov 19 18:36:47 2012 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Mon Nov 19 18:36:50 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> Message-ID: Sure they want more physicians. My husband was forced to leave medical school when he became sick (they wouldn't give him a medical leave of absence). No other medical school would touch him after that. They thought something was fishy, his transcript reads "medical leave of absence" which wasn't true. The other shcools can't figure out why the school he was in wouldn't take him back. If he wanted to retake the MCAT and apply cold, they told him he could do that. Medicine is a self limiting profession. The AMA determines how many doctors are availabe to keep a limited resource, limited. Thus the $280/hour radiologist. You leave with close to $200,000 in debt if attending a private school. Primary care pays, in most regions, under $100,000/yr. When you have that kind of debt, lower paying primary care jobs aren't enticing. My bitter two cents. Paula On Mon, Nov 19, 2012 at 4:25 PM, Webster, Thomas S. wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Mon Nov 19 18:50:41 2012 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Mon Nov 19 18:50:54 2012 Subject: [Histonet] Need parts for old equipment Message-ID: Hello all - I have been volunteering in a lab with very old equipment. Although I was able to find 2 processors, they have both "died". We do not have the money to buy even a used processor, so I am looking for refurbished parts. I am hoping there are some vendors out there who have parts that we need or if there is a lab with a processor that works that they don't want we might be able to pay shipping. I have a VIP 3000 and need a motor, gear head, bearing washer and roller washer. The other processor is a Tissue-Tek II (c. 1983). I think I need a optoelectronic switch. Any help any one of you can give me, contact me personally. Thank you in advance, Cheryl Crowder, BA, HTL(ASCP) Animal Science Department LSU Baton Rouge, LA 70820 (225) 772-2865 From histotalk <@t> yahoo.com Mon Nov 19 20:49:15 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon Nov 19 20:49:19 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <-3423156224104217588@unknownmsgid> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> Message-ID: <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Nov 20 02:26:01 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 20 02:26:27 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org>, Message-ID: Wow, I guess I should have considered radiology when I was going to college! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 19 Nov 2012 15:56:26 -0600 > From: jaylundgren@gmail.com > To: twebster@crh.org > Subject: Re: [Histonet] 88305TC starting to hit the fan... > CC: histonet@lists.utsouthwestern.edu > > Because there is a huge shortage of primary care docs as it is, which will > only get worse when millions of uninsured people suddenly have insurance. > So I guess they are trying to entice more med students into primary care. > There is a push to train more physician's assistants too > > As it is, the top medical students want to go into something like radiology > or dermatology so they can work banker's hours and make 5x as much money as > a PCHP, and who can blame them? > > I friend of mine who is a radiology tech saw a locum radiologist's pay stub > left on the fax machine (by accident), and said radiologist makes $280./hr. > > Jay A. Lundgren, M.S., HTL (ASCP) > > > On Mon, Nov 19, 2012 at 3:25 PM, Webster, Thomas S. wrote: > > > CAP had a webinar last week about the cut. These are some very scary > > times. For some reason the government has decided to shift wealth from > > specialists to family practice. I am becoming more angry with the > > affordable care act everyday. > > > > > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > > > > CONFIDENTIALITY NOTICE: > > This e-mail message, including all attachments, is for the sole use of the > > intended recipient(s) and may contain confidential and privileged > > information. You may NOT use, disclose, copy or disseminate this > > information. If you are not the intended recipient, please contact the > > sender by reply e-mail immediately. Please destroy all copies of the > > original message and all attachments. Your cooperation is greatly > > appreciated. > > Columbus Regional Hospital > > 2400 East 17th Street > > Columbus, Indiana 47201_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Tue Nov 20 07:10:47 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Nov 20 07:10:58 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org><-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: This statement is ridiculous and not worth responding to further than to say, clearly you don't understand the CMS process. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Monday, November 19, 2012 9:49 PM To: Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rjbuesa <@t> yahoo.com Tue Nov 20 07:54:45 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 20 07:54:52 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <-3423156224104217588@unknownmsgid> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> Message-ID: <1353419685.62724.YahooMailNeo@web163104.mail.bf1.yahoo.com> Very well said! Ren? J. ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 20 07:58:22 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 20 07:58:31 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Nov 20 08:00:41 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Nov 20 08:00:50 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 20 08:04:23 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 20 08:04:33 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> Message-ID: <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> The law provides economic assistance to those?with low?income who cannot afford insurance. It would be nice if we all read the law instead of paying attention to those who try to scare people. Ren? J. ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 9:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wakehealth.edu Tue Nov 20 08:11:50 2012 From: hborgeri <@t> wakehealth.edu (Hermina Borgerink) Date: Tue Nov 20 08:12:08 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <1199C3D2DD5872438C656168966E3265016E6D94@EXCHDB2.medctr.ad.wfubmc.edu> Thank you Ren?. Couldn't agree more. As a Stage 3C ovarian cancer patient, I would be bankrupt right now if not for insurance. I've been on/off chemotherapy for the past five years, and at nearly $4,000.00 a treatment, not to mention nearly $200,000.00 in surgery costs ....... The monthly cost of insurance pales in comparison to the cost of a catastrophic illness. Hermina M. Borgerink, MA, HT(ASCP)HTL, QIHC Wake Forest University Primate Center Department of Pathology Medical Center Blvd. Winston-Salem, N C? 27157 Tel. (336) 716-1538 Fax (336) 716-1515 Email: hborgeri@wakehealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 8:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Tue Nov 20 08:27:56 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Nov 20 08:28:06 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353419685.62724.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org><-3423156224104217588@unknownmsgid> <1353419685.62724.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: Agreed! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 8:55 AM To: Davide Costanzo; Webster, Thomas S. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 88305TC starting to hit the fan... Very well said! Ren? J. ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Lynn.Burton <@t> Illinois.gov Tue Nov 20 08:31:31 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Nov 20 08:31:42 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: Amen. I never voted for him -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Monday, November 19, 2012 8:49 PM To: Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> virtua.org Tue Nov 20 08:35:10 2012 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Tue Nov 20 08:35:17 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <6932520047F7EE46B512E9801344F160026314@ExchangeMB-1.Virtua.org> And what about NO INCOME................an issue that many are currently facing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 9:04 AM To: Bernice Frederick; David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... The law provides economic assistance to those?with low?income who cannot afford insurance. It would be nice if we all read the law instead of paying attention to those who try to scare people. Ren? J. ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 9:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From dmborel <@t> aol.com Tue Nov 20 08:44:12 2012 From: dmborel <@t> aol.com (Dmborel) Date: Tue Nov 20 08:44:21 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org>, Message-ID: <8CF9544A406F769-10E0-2D736@webmail-m170.sysops.aol.com> If any of the histotechs who read these posts are in a situation where their local pathology lab may be closed because of Medicare cuts, let me know your contact info. Perhaps I could do something to keep that lab closure from happening. David M. Borel, MD Pathology Services, P.A./Dermatopathology Diagnostics 5650 SW 29th Street Topeka, KS 66614 Office 785-272-8246 Cell 785-249-2860 -----Original Message----- From: joelle weaver To: jaylundgren ; twebster Cc: histonet Sent: Tue, Nov 20, 2012 2:27 am Subject: RE: [Histonet] 88305TC starting to hit the fan... ow, I guess I should have considered radiology when I was going to college! oelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 19 Nov 2012 15:56:26 -0600 From: jaylundgren@gmail.com To: twebster@crh.org Subject: Re: [Histonet] 88305TC starting to hit the fan... CC: histonet@lists.utsouthwestern.edu Because there is a huge shortage of primary care docs as it is, which will only get worse when millions of uninsured people suddenly have insurance. So I guess they are trying to entice more med students into primary care. There is a push to train more physician's assistants too As it is, the top medical students want to go into something like radiology or dermatology so they can work banker's hours and make 5x as much money as a PCHP, and who can blame them? I friend of mine who is a radiology tech saw a locum radiologist's pay stub left on the fax machine (by accident), and said radiologist makes $280./hr. Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Nov 19, 2012 at 3:25 PM, Webster, Thomas S. wrote: > CAP had a webinar last week about the cut. These are some very scary > times. For some reason the government has decided to shift wealth from > specialists to family practice. I am becoming more angry with the > affordable care act everyday. > > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Nov 20 08:48:42 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Nov 20 08:48:54 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: There are caps on what folks must pay, folks... please read the law. There are many good things about the new law. Also realize that all of the changes we're facing are not part of Obamacare. The Grandfather clause has been ending for years. Congress finally let it this year. Something had to change and change is hard. Let's work together with our Congress to adjust what needs to be adjusted as needed. Maybe they will begin to work together if we demand it. Happy Thanksgiving - we still have much to be thankful for!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 9:04 AM To: Bernice Frederick; David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... The law provides economic assistance to those with low income who cannot afford insurance. It would be nice if we all read the law instead of paying attention to those who try to scare people. Ren? J. ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 9:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. Yours, David ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact > the sender by reply e-mail immediately. Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From NMP <@t> stowers.org Tue Nov 20 09:03:28 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Tue Nov 20 09:03:40 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF90790705FD3@EXCHMB-02.stowers-institute.org> Very well said Joyce. Thank you. Happy Thanksgiving to you. Best regards. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, November 20, 2012 8:49 AM To: 'Rene J Buesa'; Bernice Frederick; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... There are caps on what folks must pay, folks... please read the law. There are many good things about the new law. Also realize that all of the changes we're facing are not part of Obamacare. The Grandfather clause has been ending for years. Congress finally let it this year. Something had to change and change is hard. Let's work together with our Congress to adjust what needs to be adjusted as needed. Maybe they will begin to work together if we demand it. Happy Thanksgiving - we still have much to be thankful for!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 9:04 AM To: Bernice Frederick; David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... The law provides economic assistance to those with low income who cannot afford insurance. It would be nice if we all read the law instead of paying attention to those who try to scare people. Ren? J. ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 9:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. Yours, David ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact > the sender by reply e-mail immediately. Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Tue Nov 20 09:03:03 2012 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Tue Nov 20 09:03:52 2012 Subject: [Histonet] Question on frozen section IHC and tissue adhesion Message-ID: Hi all, I am performing IHC on tissue that was formalin fixed for 48 hours and then embedded in OCT. I have been using Gold Plus slides from Thermo Fisher. I am staining rat spleen and mandibular lymph nodes. With a peroxidase method, the tissue completely digests off the slides as soon as I apply the peroxidase block (looks like pouring hydrogen peroxide on a cut - just turns white and bubbles off of the slide). I have tried various concentrations of peroxidase block with no luck. With the alkaline phos method, the tissue is staying adhered to the slide, but I am getting significant 'tissue lifting' which makes the pathologists interpretation very difficult. Is there anyone out there with experience in doing IHC on formalin fixed frozen sections that has experienced similar 'tissue lifting' problems? I have ran this antibody on this tissue with immunofluorescence successfully - that may be the only choice we have for this particular tissue. Would really like to be able to perform this staining with either an HRP or AP method for light microscopy if possible. Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From joewalker <@t> rrmc.org Tue Nov 20 09:23:39 2012 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Nov 20 09:23:49 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF90790705FD3@EXCHMB-02.stowers-institute.org> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> <2C40E43D1F7A56408C4463FD245DDDF90790705FD3@EXCHMB-02.stowers-institute.org> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1794F70B@RRMBX03.rrmc.local> Let's be a little factual. The discussion to cut 88305 has been going on for years, even before the Accountable Care Act was passed. Lab fees (TC) and professional fees (PC) in general have been under the gun for cuts year after year. Health care is changing due to the unsustainable costs associated with providing services. The ACA did give the government a reason to take a hard look at many of the codes. 88305 hadn't been evaluated closely since 1999. It is debatable as to what data was used to develop a cost analysis for the work done to determine the cuts. 88305 is being cut by 51.7% on the TC side. Interestingly, the PC(26) side is being increased by 1.9%. Likewise, 88300 is being but 57.1%, 88302 is being cut 51% and 88304 is being cut 34.7%. All of them have slight increases for the PC side. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Tuesday, November 20, 2012 10:03 AM To: 'Weems, Joyce K.'; 'Rene J Buesa'; Bernice Frederick; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... Very well said Joyce. Thank you. Happy Thanksgiving to you. Best regards. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, November 20, 2012 8:49 AM To: 'Rene J Buesa'; Bernice Frederick; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... There are caps on what folks must pay, folks... please read the law. There are many good things about the new law. Also realize that all of the changes we're facing are not part of Obamacare. The Grandfather clause has been ending for years. Congress finally let it this year. Something had to change and change is hard. Let's work together with our Congress to adjust what needs to be adjusted as needed. Maybe they will begin to work together if we demand it. Happy Thanksgiving - we still have much to be thankful for!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 9:04 AM To: Bernice Frederick; David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... The law provides economic assistance to those with low income who cannot afford insurance. It would be nice if we all read the law instead of paying attention to those who try to scare people. Ren? J. ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 9:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. Yours, David ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact > the sender by reply e-mail immediately. Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From contact <@t> excaliburpathology.com Tue Nov 20 09:24:20 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Nov 20 09:24:28 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> Message-ID: <1353425060.59732.YahooMailNeo@web5710.biz.mail.ne1.yahoo.com> Liverpool Care Pathway. Google it. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 8:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 20 09:38:07 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 20 09:38:11 2012 Subject: [Histonet] Question on frozen section IHC and tissue adhesion In-Reply-To: References: Message-ID: <1353425887.56070.YahooMailNeo@web163104.mail.bf1.yahoo.com> Formalin fixed tissue is not adequate for frozen sectioning unless you place it in sucrose first before going into OCT and frozen sectioning. Spleen is particularly difficult for its high blood contents. Ideally you should try to obtain unfixed tissue. Ren? J. ________________________________ From: "Thurby, Christina" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 20, 2012 10:03 AM Subject: [Histonet] Question on frozen section IHC and tissue adhesion Hi all, I am performing IHC on tissue that was formalin fixed for 48 hours and then embedded in OCT.? I have been using Gold Plus slides from Thermo Fisher.? ? I am staining rat spleen and mandibular lymph nodes.? With a peroxidase method, the tissue completely digests off the slides as soon as I apply the peroxidase block (looks like pouring hydrogen peroxide on a cut - just turns white and bubbles off of the slide).? I have tried various concentrations of peroxidase block with no luck.? With the alkaline phos method, the tissue is staying adhered to the slide, but I am getting significant 'tissue lifting' which makes the pathologists interpretation very difficult.? Is there anyone out there with experience in doing IHC on formalin fixed frozen sections that has experienced similar 'tissue lifting' problems?? I have ran this antibody on this tissue with immunofluorescence successfully - that may be the only choice we have for this particular tissue.? Would really like to be able to perform this staining with either an HRP or AP method for light microscopy if possible. Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Tue Nov 20 09:40:04 2012 From: ihcman2010 <@t> hotmail.com (=?utf-8?B?aWhjbWFuMjAxMEBob3RtYWlsLmNvbQ==?=) Date: Tue Nov 20 09:39:59 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gODgzMDVUQyBzdGFydGluZyB0byBoaXQgdGhlIGZhbi4uLg==?= Message-ID: We can all see sour grapes about the election by simply turning on any news channel. Histonet should stay free of those entanglements and remain focused on discussion and problem solving. My opinion. Glen Dawson Janesville, WI Sent from my U.S. Cellular Android device ----- Reply message ----- From: "David Kemler" Date: Mon, Nov 19, 2012 8:49 pm Subject: [Histonet] 88305TC starting to hit the fan... To: "Fellow HistoNetters" Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JStanicar <@t> communitymed.org Tue Nov 20 09:52:38 2012 From: JStanicar <@t> communitymed.org (Stanicar, Jennifer) Date: Tue Nov 20 09:52:51 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: References: Message-ID: Thank you for that Glen. I agree! Jen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcman2010@hotmail.com Sent: Tuesday, November 20, 2012 8:40 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... We can all see sour grapes about the election by simply turning on any news channel. Histonet should stay free of those entanglements and remain focused on discussion and problem solving. My opinion. Glen Dawson Janesville, WI Sent from my U.S. Cellular Android device ----- Reply message ----- From: "David Kemler" Date: Mon, Nov 19, 2012 8:49 pm Subject: [Histonet] 88305TC starting to hit the fan... To: "Fellow HistoNetters" Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact the > sender by reply e-mail immediately.? Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Nov 20 10:00:50 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Nov 20 10:01:13 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC1794F70B@RRMBX03.rrmc.local> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> <2C40E43D1F7A56408C4463FD245DDDF90790705FD3@EXCHMB-02.stowers-institute.org> <3C2378778400AD448ADA6FD6BDB7CCCC1794F70B@RRMBX03.rrmc.local> Message-ID: CAP has a webinar on the web site that explains all this pretty well. Seems like the reimbursement was increased for 88307 and 88309, so that the overall decrease is 6% if I understood correctly. I need to meditate on it a bit. The problem with the 88305 code is that clinicians have taken it away from pathologists... I've been harping for years.. prostates being the biggest culprit for price increase. Shame, shame, shame - in Gomer Pyle speak.. !! :>) Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Joe W. Walker, Jr. [mailto:joewalker@rrmc.org] Sent: Tuesday, November 20, 2012 10:24 AM To: Marsh, Nannette; Weems, Joyce K.; 'Rene J Buesa'; Bernice Frederick; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... Let's be a little factual. The discussion to cut 88305 has been going on for years, even before the Accountable Care Act was passed. Lab fees (TC) and professional fees (PC) in general have been under the gun for cuts year after year. Health care is changing due to the unsustainable costs associated with providing services. The ACA did give the government a reason to take a hard look at many of the codes. 88305 hadn't been evaluated closely since 1999. It is debatable as to what data was used to develop a cost analysis for the work done to determine the cuts. 88305 is being cut by 51.7% on the TC side. Interestingly, the PC(26) side is being increased by 1.9%. Likewise, 88300 is being but 57.1%, 88302 is being cut 51% and 88304 is being cut 34.7%. All of them have slight increases for the PC side. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Tuesday, November 20, 2012 10:03 AM To: 'Weems, Joyce K.'; 'Rene J Buesa'; Bernice Frederick; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... Very well said Joyce. Thank you. Happy Thanksgiving to you. Best regards. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, November 20, 2012 8:49 AM To: 'Rene J Buesa'; Bernice Frederick; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... There are caps on what folks must pay, folks... please read the law. There are many good things about the new law. Also realize that all of the changes we're facing are not part of Obamacare. The Grandfather clause has been ending for years. Congress finally let it this year. Something had to change and change is hard. Let's work together with our Congress to adjust what needs to be adjusted as needed. Maybe they will begin to work together if we demand it. Happy Thanksgiving - we still have much to be thankful for!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 9:04 AM To: Bernice Frederick; David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... The law provides economic assistance to those with low income who cannot afford insurance. It would be nice if we all read the law instead of paying attention to those who try to scare people. Ren? J. ________________________________ From: Bernice Frederick To: Rene J Buesa ; David Kemler ; Fellow HistoNetters Sent: Tuesday, November 20, 2012 9:00 AM Subject: RE: [Histonet] 88305TC starting to hit the fan... But can they afford to buy it???? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. Ren? J. ________________________________ From: David Kemler To: Fellow HistoNetters Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. Yours, David ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact > the sender by reply e-mail immediately. Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From akbitting <@t> geisinger.edu Tue Nov 20 10:02:41 2012 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Nov 20 10:02:54 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F60BCCB5C3@GHSEXMBX2W8K1V.geisinger.edu> "Histonet should stay free of those entanglements(political opinions)" Funny, when I wrote that here 2 weeks before the election no one wanted to hear it. What has changed?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stanicar, Jennifer Sent: Tuesday, November 20, 2012 10:53 AM To: 'ihcman2010@hotmail.com'; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... Thank you for that Glen. I agree! Jen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcman2010@hotmail.com Sent: Tuesday, November 20, 2012 8:40 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... We can all see sour grapes about the election by simply turning on any news channel. Histonet should stay free of those entanglements and remain focused on discussion and problem solving. My opinion. Glen Dawson Janesville, WI Sent from my U.S. Cellular Android device ----- Reply message ----- From: "David Kemler" Date: Mon, Nov 19, 2012 8:49 pm Subject: [Histonet] 88305TC starting to hit the fan... To: "Fellow HistoNetters" Hmmm...I think more people should have paid attention to Obamacare two years ago when it?was being?shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed?that there are so many in the?profession who?are surprised?about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) ? Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. ? Yours, David ? ? ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl > t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar > s.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information.? If you are not the intended recipient, please contact > the sender by reply e-mail immediately.? Please destroy all copies of > the original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From twebster <@t> CRH.org Tue Nov 20 10:12:48 2012 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Tue Nov 20 10:12:58 2012 Subject: [Histonet] 88305TC starting to hit the fan... Message-ID: <7207186ED68FB542803CAF1CE6E82FF80348D0@exmb1.crh.org> I won't disagree that family practice is low paying relative to some specialists. But, pathologists and laboratories already do enough to help the income of family practice physicians. Think about how much money they make from client billing the lab work. And now we have to put up with these huge cuts? Just doesn't seem fair. Why doesn't the government throw us a bone and make nationwide laws that ONLY the entity providing a service shall bill for it? Then we wouldn't have to deal with the profit sharing that goes on with client billing. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From TJohnson <@t> gnf.org Tue Nov 20 10:17:18 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Nov 20 10:17:27 2012 Subject: [Histonet] Re: Question on frozen section IHC and tissue adhesion Message-ID: <9F3CFEE76E51B64991C7485270890B400CE10BEF@EX4.lj.gnf.org> Hi Christina, I agree with Rene Buesa that ideally you should have added a cryoprotection step prior to freezing. If you snap freeze quickly enough though, you might have gotten away with no freezing artifact. An H&E will tell you that. If it looks like a sponge, or like frost patterns, you might have a specimen that is useless. If not, proceed. You might try a couple things. First, cut as thin as you can. 4 microns if you can get away with it. Air dry at least one hour after sectioning, preferably overnight. If you use peroxidase block, dilute your solution to 0.3% and increase your time to 30 minutes. This provides a more gentle approach to endogenous peroxidase blocking. If you can keep the sections on by doing the first two things listed, you can probably use the Alk Phos detection method and not have to worry about using H2O2. What is the difference in your fluorescence and your chromogen technique besides the H2O2? Fewer steps and washes? Make sure your buffer rinses are done gently, maybe in a coplin jar instead of squirting with a squeeze bottle. Good luck! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From Sadeq.Kharzai <@t> nationwidechildrens.org Tue Nov 20 10:35:13 2012 From: Sadeq.Kharzai <@t> nationwidechildrens.org (Kharzai, Sadeq) Date: Tue Nov 20 10:35:27 2012 Subject: [Histonet] remve from the list Message-ID: Would you please remove my name and e -address from the histonet list/ Thank you, Sadeq Kharzai ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From BGapinski <@t> pathgroup.com Tue Nov 20 10:36:39 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Tue Nov 20 10:36:45 2012 Subject: [Histonet] Pa Leeeze Message-ID: Wow, How disappointing. Looking for constructive ways to keep my lab open and I get political stuff. Did you all go crazy in the 80's with Ronald Ray-gun and the DRG's? Too young? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From peter.craven <@t> nhs.net Tue Nov 20 10:49:19 2012 From: peter.craven <@t> nhs.net (Craven Peter (NHS HIGHLAND)) Date: Tue Nov 20 10:50:40 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <20121120155327.11A7E44A477@nhs-pd1e-esg010.ad1.nhs.net> References: , <20121120155327.11A7E44A477@nhs-pd1e-esg010.ad1.nhs.net> Message-ID: <20121120165031.79510449098@nhs-pd1e-esg103.ad1.nhs.net> Me too!! Mind you I am not in the states and not in a private health care service job. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital Old Perth Road Inverness IV2 3UJ Tel 01463 704269 email peter.craven@nhs.net ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stanicar, Jennifer [JStanicar@communitymed.org] Sent: 20 November 2012 03:52 PM To: 'ihcman2010@hotmail.com'; David Kemler; Fellow HistoNetters Subject: RE: [Histonet] 88305TC starting to hit the fan... Thank you for that Glen. I agree! Jen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcman2010@hotmail.com Sent: Tuesday, November 20, 2012 8:40 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... We can all see sour grapes about the election by simply turning on any news channel. Histonet should stay free of those entanglements and remain focused on discussion and problem solving. My opinion. Glen Dawson Janesville, WI Sent from my U.S. Cellular Android device ----- Reply message ----- From: "David Kemler" Date: Mon, Nov 19, 2012 8:49 pm Subject: [Histonet] 88305TC starting to hit the fan... To: "Fellow HistoNetters" Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. Yours, David ________________________________ From: Davide Costanzo To: "Webster, Thomas S." Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr > > > CONFIDENTIALITY NOTICE: > This e-mail message, including all attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. You may NOT use, disclose, copy or disseminate this > information. If you are not the intended recipient, please contact the > sender by reply e-mail immediately. Please destroy all copies of the > original message and all attachments. Your cooperation is greatly > appreciated. > Columbus Regional Hospital > 2400 East 17th Street > Columbus, Indiana 47201_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From pathlocums <@t> gmail.com Tue Nov 20 09:31:14 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Nov 20 10:54:44 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <-6555410300214361670@unknownmsgid> Tell them Rene! Too many Fox News listeners here I suspect. Sent from my iPhone On Nov 20, 2012, at 6:05 AM, Rene J Buesa wrote: > The law provides economic assistance to those with low income who cannot afford insurance. > It would be nice if we all read the law instead of paying attention to those who try to scare people. > Ren? J. > > > ________________________________ > From: Bernice Frederick > To: Rene J Buesa ; David Kemler ; Fellow HistoNetters > Sent: Tuesday, November 20, 2012 9:00 AM > Subject: RE: [Histonet] 88305TC starting to hit the fan... > > But can they afford to buy it???? > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, November 20, 2012 7:58 AM > To: David Kemler; Fellow HistoNetters > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Tell that to all those who have pre-existing medical conditions that can now buy health insurance. > Ren? J. > > > ________________________________ > From: David Kemler > To: Fellow HistoNetters > Sent: Monday, November 19, 2012 9:49 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) > > Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. > > Yours, > David > > > > > ________________________________ > From: Davide Costanzo > To: "Webster, Thomas S." > Cc: "histonet@lists.utsouthwestern.edu" > Sent: Monday, November 19, 2012 6:46 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. > Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. > > Sent from my iPhone > > On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > >> CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. >> >> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid >> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl >> t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar >> s.html&_state=maximized&_pageLabel=cntvwr >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use of >> the intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact >> the sender by reply e-mail immediately. Please destroy all copies of >> the original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Tue Nov 20 09:34:18 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Nov 20 10:54:48 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <5847839503195312806@unknownmsgid> Nicely stated! Sent from my iPhone On Nov 20, 2012, at 6:49 AM, "Weems, Joyce K." wrote: > There are caps on what folks must pay, folks... please read the law. There are many good things about the new law. > > Also realize that all of the changes we're facing are not part of Obamacare. The Grandfather clause has been ending for years. Congress finally let it this year. > > Something had to change and change is hard. Let's work together with our Congress to adjust what needs to be adjusted as needed. Maybe they will begin to work together if we demand it. > > Happy Thanksgiving - we still have much to be thankful for!! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, November 20, 2012 9:04 AM > To: Bernice Frederick; David Kemler; Fellow HistoNetters > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > The law provides economic assistance to those with low income who cannot afford insurance. > It would be nice if we all read the law instead of paying attention to those who try to scare people. > Ren? J. > > > ________________________________ > From: Bernice Frederick > To: Rene J Buesa ; David Kemler ; Fellow HistoNetters > Sent: Tuesday, November 20, 2012 9:00 AM > Subject: RE: [Histonet] 88305TC starting to hit the fan... > > But can they afford to buy it???? > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, November 20, 2012 7:58 AM > To: David Kemler; Fellow HistoNetters > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Tell that to all those who have pre-existing medical conditions that can now buy health insurance. > Ren? J. > > > ________________________________ > From: David Kemler > To: Fellow HistoNetters > Sent: Monday, November 19, 2012 9:49 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) > > Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. > > Yours, > David > > > > > ________________________________ > From: Davide Costanzo > To: "Webster, Thomas S." > Cc: "histonet@lists.utsouthwestern.edu" > Sent: Monday, November 19, 2012 6:46 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. > Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. > > Sent from my iPhone > > On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > >> CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. >> >> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid >> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl >> t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar >> s.html&_state=maximized&_pageLabel=cntvwr >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use of >> the intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact >> the sender by reply e-mail immediately. Please destroy all copies of >> the original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Tue Nov 20 09:29:15 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Nov 20 10:54:52 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: <416152486713122595@unknownmsgid> This is not a political forum. Keep your anti-President talk off this site. Sent from my iPhone On Nov 19, 2012, at 6:49 PM, David Kemler wrote: > Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes..."You ain't seen nottin' yet"! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 & 14 and all of them affect your job or lack thereof. :) > > Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. > > Yours, > David > > > > > ________________________________ > From: Davide Costanzo > To: "Webster, Thomas S." > Cc: "histonet@lists.utsouthwestern.edu" > Sent: Monday, November 19, 2012 6:46 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > While this stinks on many levels, I have to take issue with the "shift > wealth from specialists to family practice" - family practice docs > have been the frontline of medicine, all the while earning less than a > quarter of what specialists earn. It's about time they get a boost. > Too many specialists earn over a million a year, while the family > practice guys/ladies can barely pay their student loans. > > Sent from my iPhone > > On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > >> CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. >> >> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html&_state=maximized&_pageLabel=cntvwr >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use of the >> intended recipient(s) and may contain confidential and privileged >> information. You may NOT use, disclose, copy or disseminate this >> information. If you are not the intended recipient, please contact the >> sender by reply e-mail immediately. Please destroy all copies of the >> original message and all attachments. Your cooperation is greatly >> appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Nov 20 11:01:08 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Nov 20 11:01:25 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <-6555410300214361670@unknownmsgid> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> <-6555410300214361670@unknownmsgid> Message-ID: Pardon me? What do you mean by that? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, November 20, 2012 10:31 AM To: Rene J Buesa Cc: Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell them Rene! Too many Fox News listeners here I suspect. Sent from my iPhone On Nov 20, 2012, at 6:05 AM, Rene J Buesa wrote: > The law provides economic assistance to those with low income who cannot afford insurance. > It would be nice if we all read the law instead of paying attention to those who try to scare people. > Ren? J. > > > ________________________________ > From: Bernice Frederick > To: Rene J Buesa ; David Kemler > ; Fellow HistoNetters > > Sent: Tuesday, November 20, 2012 9:00 AM > Subject: RE: [Histonet] 88305TC starting to hit the fan... > > But can they afford to buy it???? > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Tuesday, November 20, 2012 7:58 AM > To: David Kemler; Fellow HistoNetters > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Tell that to all those who have pre-existing medical conditions that can now buy health insurance. > Ren? J. > > > ________________________________ > From: David Kemler > To: Fellow HistoNetters > Sent: Monday, November 19, 2012 9:49 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Hmmm...I think more people should have paid attention to Obamacare two > years ago when it was being shoved down eveyones throat. Oh well.. as > old saying goes..."You ain't seen nottin' yet"! I'm just amazed that > there are so many in the profession who are surprised about the > changes beginning to take place, there are many, many more to come in > 2013 & 14 and all of them affect your job or lack thereof. :) > > Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. > > Yours, > David > > > > > ________________________________ > From: Davide Costanzo > To: "Webster, Thomas S." > Cc: "histonet@lists.utsouthwestern.edu" > > Sent: Monday, November 19, 2012 6:46 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. > Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. > > Sent from my iPhone > > On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > >> CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. >> >> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverri >> d >> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPt >> l >> t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webina >> r s.html&_state=maximized&_pageLabel=cntvwr >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use >> of the intended recipient(s) and may contain confidential and >> privileged information. You may NOT use, disclose, copy or >> disseminate this information. If you are not the intended recipient, >> please contact the sender by reply e-mail immediately. Please >> destroy all copies of the original message and all attachments. Your >> cooperation is greatly appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana >> 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Nov 20 11:05:15 2012 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Nov 20 11:05:27 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <-6555410300214361670@unknownmsgid> References: <7207186ED68FB542803CAF1CE6E82FF8034860@exmb1.crh.org> <-3423156224104217588@unknownmsgid> <1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com> <1353419902.3539.YahooMailNeo@web163106.mail.bf1.yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1F9B53B5@evcspmbx2.ads.northwestern.edu> <1353420263.84188.YahooMailNeo@web163105.mail.bf1.yahoo.com> <-6555410300214361670@unknownmsgid> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F60BCCB6FD@GHSEXMBX2W8K1V.geisinger.edu> Are we a bunch of catty 13 year olds or can we stick to Healthcare? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, November 20, 2012 10:31 AM To: Rene J Buesa Cc: Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell them Rene! Too many Fox News listeners here I suspect. Sent from my iPhone On Nov 20, 2012, at 6:05 AM, Rene J Buesa wrote: > The law provides economic assistance to those with low income who cannot afford insurance. > It would be nice if we all read the law instead of paying attention to those who try to scare people. > Ren? J. > > > ________________________________ > From: Bernice Frederick > To: Rene J Buesa ; David Kemler > ; Fellow HistoNetters > > Sent: Tuesday, November 20, 2012 9:00 AM > Subject: RE: [Histonet] 88305TC starting to hit the fan... > > But can they afford to buy it???? > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Tuesday, November 20, 2012 7:58 AM > To: David Kemler; Fellow HistoNetters > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Tell that to all those who have pre-existing medical conditions that can now buy health insurance. > Ren? J. > > > ________________________________ > From: David Kemler > To: Fellow HistoNetters > Sent: Monday, November 19, 2012 9:49 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > Hmmm...I think more people should have paid attention to Obamacare two > years ago when it was being shoved down eveyones throat. Oh well.. as > old saying goes..."You ain't seen nottin' yet"! I'm just amazed that > there are so many in the profession who are surprised about the > changes beginning to take place, there are many, many more to come in > 2013 & 14 and all of them affect your job or lack thereof. :) > > Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. > > Yours, > David > > > > > ________________________________ > From: Davide Costanzo > To: "Webster, Thomas S." > Cc: "histonet@lists.utsouthwestern.edu" > > Sent: Monday, November 19, 2012 6:46 PM > Subject: Re: [Histonet] 88305TC starting to hit the fan... > > While this stinks on many levels, I have to take issue with the "shift wealth from specialists to family practice" - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. > Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. > > Sent from my iPhone > > On Nov 19, 2012, at 1:25 PM, "Webster, Thomas S." wrote: > >> CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. >> >> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverri >> d >> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPt >> l >> t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webina >> r s.html&_state=maximized&_pageLabel=cntvwr >> >> >> CONFIDENTIALITY NOTICE: >> This e-mail message, including all attachments, is for the sole use >> of the intended recipient(s) and may contain confidential and >> privileged information. You may NOT use, disclose, copy or >> disseminate this information. If you are not the intended recipient, >> please contact the sender by reply e-mail immediately. Please >> destroy all copies of the original message and all attachments. Your >> cooperation is greatly appreciated. >> Columbus Regional Hospital >> 2400 East 17th Street >> Columbus, Indiana >> 47201_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From SteveM <@t> mcclainlab.com Tue Nov 20 12:27:52 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Tue Nov 20 12:28:24 2012 Subject: [Histonet] 88305 Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF3055F182@ML1.McClainLabs.local> Looks like rough seas ahead. Raising my research fees 33%. Investing to start a new patient center to do more procedures. After that we may have to get creative. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From CDavis <@t> che-east.org Tue Nov 20 12:46:18 2012 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Nov 20 12:46:32 2012 Subject: [Histonet] RE: Histonet Digest, Vol 108, Issue 26 In-Reply-To: References: Message-ID: <08861B9CF6C7774E874635A4818AE37B03791DE7@CHEXCMS01.one.ads.che.org> Hi Christina, try post fixing the FS slides in ice cold acetone (in a freezer) for 30 minutes. That is what was reccommend to me when I was trying to do IHC on ThinPrep slides. Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page ________________________________________ RE: Hi all, I am performing IHC on tissue that was formalin fixed for 48 hours and then embedded in OCT. I have been using Gold Plus slides from Thermo Fisher. I am staining rat spleen and mandibular lymph nodes. With a peroxidase method, the tissue completely digests off the slides as soon as I apply the peroxidase block (looks like pouring hydrogen peroxide on a cut - just turns white and bubbles off of the slide). I have tried various concentrations of peroxidase block with no luck. With the alkaline phos method, the tissue is staying adhered to the slide, but I am getting significant 'tissue lifting' which makes the pathologists interpretation very difficult. Is there anyone out there with experience in doing IHC on formalin fixed frozen sections that has experienced similar 'tissue lifting' problems? I have ran this antibody on this tissue with immunofluorescence successfully - that may be the only choice we have for this particular tissue. Would really like to be able to perform this staining with either an HRP or AP method for light microscopy if possible. Christina Thurby Bristol Myers Squibb 812-307-2093 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From DAndreas.Williams <@t> pbrc.edu Tue Nov 20 12:45:16 2012 From: DAndreas.Williams <@t> pbrc.edu (D'Andreas Williams) Date: Tue Nov 20 12:48:50 2012 Subject: [Histonet] RE: Help needed with mouse brain In-Reply-To: <3F0184241070BC41AD8C8EC2C45B50243A9933@pbrcas31.pbrc.edu> References: <3F0184241070BC41AD8C8EC2C45B50243A9933@pbrcas31.pbrc.edu> Message-ID: <3F0184241070BC41AD8C8EC2C45B50243A9935@pbrcas31.pbrc.edu> Greetings, We are currently trying to optimize our processing protocols for mouse tissue on a VIP 6 processor. Of particular concern to us is mouse brain processing. For the mouse brain we have noticed "fraying" of the edge of the brain. The "fraying" looks a lot like what we see when tissue is over processed. The brains cut as if they are overprocessed; they are brittle and crunch when they hit the blade. At times they roll up and create a hole in the ribbon as our histologists cuts them. Along with the "fraying" edges we noticed that the middle of the brain looks underprocessed. When the section is placed on the waterbath the section wrinkles greatly and spreads considerably, and if left longer than a few seconds explodes. We have tried several protocols, and we see this effect. The brains we are using are cut at about 4 mm thick, and they are sectioned at 5 microns at a temperature of 42 degrees. Below are links to 3 images of a brain. Image 1 shows the edge "fraying". Image 2 show the wrinkles in the tissue. The tissue was left as long as possible on the waterbath to remove wrinkles and avoid breaking apart. Image 3 shows the brain breaking apart before the wrinkles have had a chance to work their way out. Does anyone know what is causing this issue? Also, I have put together a document of all the protocols we have ran with images of the results that I can send to you. Thank you in advance. https://dl.dropbox.com/u/25427596/Histology/IMG_1682.JPG https://dl.dropbox.com/u/25427596/Histology/Wrinkles.jpg https://dl.dropbox.com/u/25427596/Histology/Center.jpg With Kindest Regards, D'Andreas Williams Research Associate Cell Biology & Bioimaging Core Pennington Biomedical Research Center dandreas.williams@pbrc.edu From billodonnell <@t> catholichealth.net Tue Nov 20 14:25:17 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Nov 20 14:25:21 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> Like it or not, politics played a part in the cut of 88305. So did POLs, CAP and a host of other factors. Finger pointing in time of uncertainty somehow makes us all feel better, but it doesn't give us concrete ways of addressing the problem. Histology has enjoyed a fairly long period of great reimbursement, reasonable per-test costs, and a certain amount of security in that what we do is unique. That is all changing, but was likely to change at least some no matter who was elected to do whatever. Remember the panic when DRG's first arrived? There is no doubt that labs are going to have to get leaner, but this was already a trend. Find reasonable ways to cut costs. I know. We've been doing this for years.... But it needs to go further. Some people will lose their jobs. I may well be one of them and I don't like it, but it is a reality. If I go down, it will not be for lack of trying to maintain. 88305 cuts are big but there are a lot of clinical services getting cuts as well. Hospitals need to do what they can to keep the doors open for the benefit of the patient. Pay cuts, bonuses+/-, benefits, hiring freezes, capital freezes are all looming on the horizon. If at all possible, fight them, but do not exhaust yourselves. It's a new world - and it will sometimes be ugly. Blame the Democrats or the Republicans, Wall Street or Main Street, but figure out how to adapt. OK. So.... What can we do to ride out the storm? 1. Find a marketing advantage. POLs and certain smaller private labs cannot remain the "bargain" they once were. My lab is expectiing to get back some of what we lost to them a few years back. We are the only game in our town.... Why are we losing business to labs in other areas? It should all be staying here. 2. Become politically active. Demand better from your elected officials and from your professional organizations that are lobbyists(sp). If they can't do the job, use your vote or your membership fees to fire them OR run for office yourself. Become an activist in your professional organization. 3. Maintain high standards. Cut-backs and performance improvement need not automatically equate to less quality. I hate it when people assume that shaving a couple of minutes must necessitate poor cutting. How close to borderline is your current quality if this is your attitude. Yes, that was snarky, but think about it. 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T PANIC. When you are caught up in a panic mentality, thinking and problem solving suffer. We need our heads in the game if we are going to come out on top. (How's that for my best Zig Zigler impersonation)? Above all - have a nice day and thank you for letting me vent a bit. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Tuesday, November 20, 2012 10:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pa Leeeze Wow, How disappointing. Looking for constructive ways to keep my lab open and I get political stuff. Did you all go crazy in the 80's with Ronald Ray-gun and the DRG's? Too young? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From pathlocums <@t> gmail.com Tue Nov 20 15:03:14 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Nov 20 15:03:18 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> Message-ID: Well put. I do think too many are panic stricken over losing a job. The work will still be there, but it might be at a different location. Physicians are not going to stop doing biopsies because the pay has been cut to the lab. The only thing that may cost a few jobs is if over-utilization is curbed. However, in my opinion that is not a large enough number to cause widespread panic among lab employees. If POL's begin closing, the work will return elsewhere. Those techs are free to apply for those jobs, and there will likely be a new job elsewhere for every one cut at your current practice. In my personal opinion, I think PA's should be far more concerned than techs. We could see a surge in the number of "grossing techs" out there, and a decline in the use of NAACLS trained PA's where the biopsy rate is high. So for all those techs that are worried, think positively - you may just experience a surge in job opportunities, and jobs with more attractive shifts. David On Tue, Nov 20, 2012 at 12:25 PM, O'Donnell, Bill < billodonnell@catholichealth.net> wrote: > Like it or not, politics played a part in the cut of 88305. So did POLs, > CAP and a host of other factors. Finger pointing in time of uncertainty > somehow makes us all feel better, but it doesn't give us concrete ways > of addressing the problem. Histology has enjoyed a fairly long period of > great reimbursement, reasonable per-test costs, and a certain amount of > security in that what we do is unique. > > That is all changing, but was likely to change at least some no matter > who was elected to do whatever. Remember the panic when DRG's first > arrived? > > There is no doubt that labs are going to have to get leaner, but this > was already a trend. Find reasonable ways to cut costs. I know. We've > been doing this for years.... But it needs to go further. > > Some people will lose their jobs. I may well be one of them and I don't > like it, but it is a reality. If I go down, it will not be for lack of > trying to maintain. > > 88305 cuts are big but there are a lot of clinical services getting cuts > as well. Hospitals need to do what they can to keep the doors open for > the benefit of the patient. Pay cuts, bonuses+/-, benefits, hiring > freezes, capital freezes are all looming on the horizon. If at all > possible, fight them, but do not exhaust yourselves. It's a new world - > and it will sometimes be ugly. Blame the Democrats or the Republicans, > Wall Street or Main Street, but figure out how to adapt. > > OK. So.... What can we do to ride out the storm? > > 1. Find a marketing advantage. POLs and certain smaller private labs > cannot remain the "bargain" they once were. My lab is expectiing to get > back some of what we lost to them a few years back. We are the only game > in our town.... Why are we losing business to labs in other areas? It > should all be staying here. > > 2. Become politically active. Demand better from your elected officials > and from your professional organizations that are lobbyists(sp). If they > can't do the job, use your vote or your membership fees to fire them OR > run for office yourself. Become an activist in your professional > organization. > > 3. Maintain high standards. Cut-backs and performance improvement need > not automatically equate to less quality. I hate it when people assume > that shaving a couple of minutes must necessitate poor cutting. How > close to borderline is your current quality if this is your attitude. > Yes, that was snarky, but think about it. > > 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T > PANIC. When you are caught up in a panic mentality, thinking and problem > solving suffer. We need our heads in the game if we are going to come > out on top. > (How's that for my best Zig Zigler impersonation)? > > Above all - have a nice day and thank you for letting me vent a bit. > > Bill > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce > Gapinski > Sent: Tuesday, November 20, 2012 10:37 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Pa Leeeze > > Wow, > How disappointing. Looking for constructive ways to keep > my lab open and I get political stuff. Did you all go crazy in the 80's > with Ronald Ray-gun and the DRG's? Too young? > > > Bruce Gapinsk HT (ASCP) > Chief Histologist > Marin Medical Laboratories > PathGroup SF > > > ________________________________ > > Important Notice: This e-mail is intended for the use of the person to > whom it is addressed and may contain information that is privileged and > confidential. If you are not the intended recipient, any disclosure, > copying, distribution, or use of the contents of this message is > strictly prohibited. If you have received this e-mail in error, please > destroy this message and contact the Security Officer at PathGroup, Inc > immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From Timothy.Morken <@t> ucsfmedctr.org Tue Nov 20 16:17:38 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Nov 20 16:17:53 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: References: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> Message-ID: <761E2B5697F795489C8710BCC72141FF02D660@ex07.net.ucsf.edu> True, things will change and despite all the angst probably for the better for medical care overall. Listen to these two podcasts from the Commonwealth Club of California for some REAL insight into the current state of medicine (or get the book of the first speaker).... 1) http://www.commonwealthclub.org/events/2012-03-19/dr-david-healy-eclipse-medical-care Dr. Otis Brawley: Fighting Patient Mistreatment in America [and by mistreatment he does not mean LACK of treatment!!] Otis Brawley, Chief Medical Officer and Executive Vice President, American Cancer Society; Co-author, How We Do Harm: A Doctor Breaks Ranks About Being Sick in America Finance, Brawley asserts, is inextricably linked to health care in America's current system. Even the very procedures patients undergo, he says, are frequently determined more by doctors' expected payment for performing them than their actual appropriateness in mitigating the ailment with which the patient is afflicted. Brawley will discuss the extent of this problem as well as possible solutions. 2) http://www.commonwealthclub.org/events/2012-03-19/dr-david-healy-eclipse-medical-care Dr. David Healy: Eclipse of Medical Care David Healy, MD, Cardiff University We live in an evidence-based medicine world. Healy sees increasing signs of a mismatch between what is passing as evidence and the real data from studies. As a result, patients and doctors are both increasingly invisible in the clinical encounter, and doctors are losing the ability to care for patients. Join us as Healy explains his theories behind this flawed system and the actions that he believes must be taken to right the problems we are facing. Tim Morken UCSF Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, November 20, 2012 1:03 PM To: O'Donnell, Bill Cc: histonet@lists.utsouthwestern.edu; Bruce Gapinski Subject: Re: [Histonet] Pa Leeeze Well put. I do think too many are panic stricken over losing a job. The work will still be there, but it might be at a different location. Physicians are not going to stop doing biopsies because the pay has been cut to the lab. The only thing that may cost a few jobs is if over-utilization is curbed. However, in my opinion that is not a large enough number to cause widespread panic among lab employees. If POL's begin closing, the work will return elsewhere. Those techs are free to apply for those jobs, and there will likely be a new job elsewhere for every one cut at your current practice. In my personal opinion, I think PA's should be far more concerned than techs. We could see a surge in the number of "grossing techs" out there, and a decline in the use of NAACLS trained PA's where the biopsy rate is high. So for all those techs that are worried, think positively - you may just experience a surge in job opportunities, and jobs with more attractive shifts. David On Tue, Nov 20, 2012 at 12:25 PM, O'Donnell, Bill < billodonnell@catholichealth.net> wrote: > Like it or not, politics played a part in the cut of 88305. So did > POLs, CAP and a host of other factors. Finger pointing in time of > uncertainty somehow makes us all feel better, but it doesn't give us > concrete ways of addressing the problem. Histology has enjoyed a > fairly long period of great reimbursement, reasonable per-test costs, > and a certain amount of security in that what we do is unique. > > That is all changing, but was likely to change at least some no matter > who was elected to do whatever. Remember the panic when DRG's first > arrived? > > There is no doubt that labs are going to have to get leaner, but this > was already a trend. Find reasonable ways to cut costs. I know. We've > been doing this for years.... But it needs to go further. > > Some people will lose their jobs. I may well be one of them and I > don't like it, but it is a reality. If I go down, it will not be for > lack of trying to maintain. > > 88305 cuts are big but there are a lot of clinical services getting > cuts as well. Hospitals need to do what they can to keep the doors > open for the benefit of the patient. Pay cuts, bonuses+/-, benefits, > hiring freezes, capital freezes are all looming on the horizon. If at > all possible, fight them, but do not exhaust yourselves. It's a new > world - and it will sometimes be ugly. Blame the Democrats or the > Republicans, Wall Street or Main Street, but figure out how to adapt. > > OK. So.... What can we do to ride out the storm? > > 1. Find a marketing advantage. POLs and certain smaller private labs > cannot remain the "bargain" they once were. My lab is expectiing to > get back some of what we lost to them a few years back. We are the > only game in our town.... Why are we losing business to labs in other > areas? It should all be staying here. > > 2. Become politically active. Demand better from your elected > officials and from your professional organizations that are > lobbyists(sp). If they can't do the job, use your vote or your > membership fees to fire them OR run for office yourself. Become an > activist in your professional organization. > > 3. Maintain high standards. Cut-backs and performance improvement need > not automatically equate to less quality. I hate it when people assume > that shaving a couple of minutes must necessitate poor cutting. How > close to borderline is your current quality if this is your attitude. > Yes, that was snarky, but think about it. > > 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T > PANIC. When you are caught up in a panic mentality, thinking and > problem solving suffer. We need our heads in the game if we are going > to come out on top. > (How's that for my best Zig Zigler impersonation)? > > Above all - have a nice day and thank you for letting me vent a bit. > > Bill > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce > Gapinski > Sent: Tuesday, November 20, 2012 10:37 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Pa Leeeze > > Wow, > How disappointing. Looking for constructive ways to > keep my lab open and I get political stuff. Did you all go crazy in > the 80's with Ronald Ray-gun and the DRG's? Too young? > > > Bruce Gapinsk HT (ASCP) > Chief Histologist > Marin Medical Laboratories > PathGroup SF > > > ________________________________ > > Important Notice: This e-mail is intended for the use of the person to > whom it is addressed and may contain information that is privileged > and confidential. If you are not the intended recipient, any > disclosure, copying, distribution, or use of the contents of this > message is strictly prohibited. If you have received this e-mail in > error, please destroy this message and contact the Security Officer at > PathGroup, Inc immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely > for the named addressee(s) and contain confidential information. If > you are not an addressee, or responsible for delivering this email to > an addressee, you have received this email in error and are notified > that reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rhonda.Gregoire <@t> gov.mb.ca Tue Nov 20 16:31:59 2012 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRI)) Date: Tue Nov 20 16:32:12 2012 Subject: [Histonet] Tissue Processors Message-ID: <52EFCA115E89304590180DA96959860944EEB4AE16@OC1EX202.ME.MBGOV.CA> Does anyone have the Thermo Shandon Excelsior or the Leica ASP300 S tissue processors? What do you like or not like about the one you have? Thanks Rhonda Gregoire, R.T. Charge Technologist Clinical Pathology/TSE Section Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Initiatives 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca From joelleweaver <@t> hotmail.com Tue Nov 20 16:34:03 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 20 16:34:07 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: References: , <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net>, Message-ID: There could be some positives- thanks for reminding us! This has been "coming down the pike" for awhile. I think that those who have been watching the "horizon" and looking ahead saw this change and many more to come. If you have been working on yourself, building your skills and knowledge, and also within your lab for awhile to work smarter and leaner, you will be prepared,-even if you still have to continue to step it up. Those who have been "sleeping at the wheel" may suffer some. But I think someone said this eariler, what we had been doing overall in healthcare could not be sustained, it was a system that was bound to flux. Politics probably have played a part in specific events, as so often is the case. But changes were coming regardless, so we might as well use our wits to work with it. Going into panic mode certainly won't help, well said by all. I will be curious to see how it plays out. I feel prepared to do what is needed. I agree getting involved in the process, and keeping a nimble mindset is a good start. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 20 Nov 2012 13:03:14 -0800 > From: pathlocums@gmail.com > To: billodonnell@catholichealth.net > Subject: Re: [Histonet] Pa Leeeze > CC: histonet@lists.utsouthwestern.edu; BGapinski@pathgroup.com > > Well put. I do think too many are panic stricken over losing a job. The > work will still be there, but it might be at a different location. > Physicians are not going to stop doing biopsies because the pay has been > cut to the lab. The only thing that may cost a few jobs is if > over-utilization is curbed. However, in my opinion that is not a large > enough number to cause widespread panic among lab employees. > > If POL's begin closing, the work will return elsewhere. Those techs are > free to apply for those jobs, and there will likely be a new job elsewhere > for every one cut at your current practice. > > In my personal opinion, I think PA's should be far more concerned than > techs. We could see a surge in the number of "grossing techs" out there, > and a decline in the use of NAACLS trained PA's where the biopsy rate is > high. So for all those techs that are worried, think positively - you may > just experience a surge in job opportunities, and jobs with more attractive > shifts. > > David > > > > > On Tue, Nov 20, 2012 at 12:25 PM, O'Donnell, Bill < > billodonnell@catholichealth.net> wrote: > > > Like it or not, politics played a part in the cut of 88305. So did POLs, > > CAP and a host of other factors. Finger pointing in time of uncertainty > > somehow makes us all feel better, but it doesn't give us concrete ways > > of addressing the problem. Histology has enjoyed a fairly long period of > > great reimbursement, reasonable per-test costs, and a certain amount of > > security in that what we do is unique. > > > > That is all changing, but was likely to change at least some no matter > > who was elected to do whatever. Remember the panic when DRG's first > > arrived? > > > > There is no doubt that labs are going to have to get leaner, but this > > was already a trend. Find reasonable ways to cut costs. I know. We've > > been doing this for years.... But it needs to go further. > > > > Some people will lose their jobs. I may well be one of them and I don't > > like it, but it is a reality. If I go down, it will not be for lack of > > trying to maintain. > > > > 88305 cuts are big but there are a lot of clinical services getting cuts > > as well. Hospitals need to do what they can to keep the doors open for > > the benefit of the patient. Pay cuts, bonuses+/-, benefits, hiring > > freezes, capital freezes are all looming on the horizon. If at all > > possible, fight them, but do not exhaust yourselves. It's a new world - > > and it will sometimes be ugly. Blame the Democrats or the Republicans, > > Wall Street or Main Street, but figure out how to adapt. > > > > OK. So.... What can we do to ride out the storm? > > > > 1. Find a marketing advantage. POLs and certain smaller private labs > > cannot remain the "bargain" they once were. My lab is expectiing to get > > back some of what we lost to them a few years back. We are the only game > > in our town.... Why are we losing business to labs in other areas? It > > should all be staying here. > > > > 2. Become politically active. Demand better from your elected officials > > and from your professional organizations that are lobbyists(sp). If they > > can't do the job, use your vote or your membership fees to fire them OR > > run for office yourself. Become an activist in your professional > > organization. > > > > 3. Maintain high standards. Cut-backs and performance improvement need > > not automatically equate to less quality. I hate it when people assume > > that shaving a couple of minutes must necessitate poor cutting. How > > close to borderline is your current quality if this is your attitude. > > Yes, that was snarky, but think about it. > > > > 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T > > PANIC. When you are caught up in a panic mentality, thinking and problem > > solving suffer. We need our heads in the game if we are going to come > > out on top. > > (How's that for my best Zig Zigler impersonation)? > > > > Above all - have a nice day and thank you for letting me vent a bit. > > > > Bill > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce > > Gapinski > > Sent: Tuesday, November 20, 2012 10:37 AM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] Pa Leeeze > > > > Wow, > > How disappointing. Looking for constructive ways to keep > > my lab open and I get political stuff. Did you all go crazy in the 80's > > with Ronald Ray-gun and the DRG's? Too young? > > > > > > Bruce Gapinsk HT (ASCP) > > Chief Histologist > > Marin Medical Laboratories > > PathGroup SF > > > > > > ________________________________ > > > > Important Notice: This e-mail is intended for the use of the person to > > whom it is addressed and may contain information that is privileged and > > confidential. If you are not the intended recipient, any disclosure, > > copying, distribution, or use of the contents of this message is > > strictly prohibited. If you have received this e-mail in error, please > > destroy this message and contact the Security Officer at PathGroup, Inc > > immediately at 615-562-9255. Thank you > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely for > > the named addressee(s) and contain confidential information. If you are not > > an addressee, or responsible for delivering this email to an addressee, you > > have received this email in error and are notified that reading, copying, > > or disclosing this email is prohibited. If you received this email in > > error, immediately reply to the sender and delete the message completely > > from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Nov 20 17:01:50 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Nov 20 17:02:04 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: References: , <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net>, Message-ID: There are cuts coming to IHC in 2014 - just be prepared for that as well. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, November 20, 2012 5:34 PM To: pathlocums@gmail.com; billodonnell@catholichealth.net Cc: histonet@lists.utsouthwestern.edu; bgapinski@pathgroup.com Subject: RE: [Histonet] Pa Leeeze There could be some positives- thanks for reminding us! This has been "coming down the pike" for awhile. I think that those who have been watching the "horizon" and looking ahead saw this change and many more to come. If you have been working on yourself, building your skills and knowledge, and also within your lab for awhile to work smarter and leaner, you will be prepared,-even if you still have to continue to step it up. Those who have been "sleeping at the wheel" may suffer some. But I think someone said this eariler, what we had been doing overall in healthcare could not be sustained, it was a system that was bound to flux. Politics probably have played a part in specific events, as so often is the case. But changes were coming regardless, so we might as well use our wits to work with it. Going into panic mode certainly won't help, well said by all. I will be curious to see how it plays out. I feel prepared to do what is needed. I agree getting involved in the process, and keeping a nimble mindset is a good start. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 20 Nov 2012 13:03:14 -0800 > From: pathlocums@gmail.com > To: billodonnell@catholichealth.net > Subject: Re: [Histonet] Pa Leeeze > CC: histonet@lists.utsouthwestern.edu; BGapinski@pathgroup.com > > Well put. I do think too many are panic stricken over losing a job. > The work will still be there, but it might be at a different location. > Physicians are not going to stop doing biopsies because the pay has > been cut to the lab. The only thing that may cost a few jobs is if > over-utilization is curbed. However, in my opinion that is not a large > enough number to cause widespread panic among lab employees. > > If POL's begin closing, the work will return elsewhere. Those techs > are free to apply for those jobs, and there will likely be a new job > elsewhere for every one cut at your current practice. > > In my personal opinion, I think PA's should be far more concerned than > techs. We could see a surge in the number of "grossing techs" out > there, and a decline in the use of NAACLS trained PA's where the > biopsy rate is high. So for all those techs that are worried, think > positively - you may just experience a surge in job opportunities, and > jobs with more attractive shifts. > > David > > > > > On Tue, Nov 20, 2012 at 12:25 PM, O'Donnell, Bill < > billodonnell@catholichealth.net> wrote: > > > Like it or not, politics played a part in the cut of 88305. So did > > POLs, CAP and a host of other factors. Finger pointing in time of > > uncertainty somehow makes us all feel better, but it doesn't give > > us concrete ways of addressing the problem. Histology has enjoyed a > > fairly long period of great reimbursement, reasonable per-test > > costs, and a certain amount of security in that what we do is unique. > > > > That is all changing, but was likely to change at least some no > > matter who was elected to do whatever. Remember the panic when DRG's > > first arrived? > > > > There is no doubt that labs are going to have to get leaner, but > > this was already a trend. Find reasonable ways to cut costs. I know. > > We've been doing this for years.... But it needs to go further. > > > > Some people will lose their jobs. I may well be one of them and I > > don't like it, but it is a reality. If I go down, it will not be for > > lack of trying to maintain. > > > > 88305 cuts are big but there are a lot of clinical services getting > > cuts as well. Hospitals need to do what they can to keep the doors > > open for the benefit of the patient. Pay cuts, bonuses+/-, benefits, > > hiring freezes, capital freezes are all looming on the horizon. If > > at all possible, fight them, but do not exhaust yourselves. It's a > > new world - and it will sometimes be ugly. Blame the Democrats or > > the Republicans, Wall Street or Main Street, but figure out how to adapt. > > > > OK. So.... What can we do to ride out the storm? > > > > 1. Find a marketing advantage. POLs and certain smaller private labs > > cannot remain the "bargain" they once were. My lab is expectiing to > > get back some of what we lost to them a few years back. We are the > > only game in our town.... Why are we losing business to labs in > > other areas? It should all be staying here. > > > > 2. Become politically active. Demand better from your elected > > officials and from your professional organizations that are > > lobbyists(sp). If they can't do the job, use your vote or your > > membership fees to fire them OR run for office yourself. Become an > > activist in your professional organization. > > > > 3. Maintain high standards. Cut-backs and performance improvement > > need not automatically equate to less quality. I hate it when people > > assume that shaving a couple of minutes must necessitate poor > > cutting. How close to borderline is your current quality if this is your attitude. > > Yes, that was snarky, but think about it. > > > > 4. Remember the mantra of the Hitchhikers Guide to the Universe: > > DON'T PANIC. When you are caught up in a panic mentality, thinking > > and problem solving suffer. We need our heads in the game if we are > > going to come out on top. > > (How's that for my best Zig Zigler impersonation)? > > > > Above all - have a nice day and thank you for letting me vent a bit. > > > > Bill > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Bruce Gapinski > > Sent: Tuesday, November 20, 2012 10:37 AM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] Pa Leeeze > > > > Wow, > > How disappointing. Looking for constructive ways to > > keep my lab open and I get political stuff. Did you all go crazy in > > the 80's with Ronald Ray-gun and the DRG's? Too young? > > > > > > Bruce Gapinsk HT (ASCP) > > Chief Histologist > > Marin Medical Laboratories > > PathGroup SF > > > > > > ________________________________ > > > > Important Notice: This e-mail is intended for the use of the person > > to whom it is addressed and may contain information that is > > privileged and confidential. If you are not the intended recipient, > > any disclosure, copying, distribution, or use of the contents of > > this message is strictly prohibited. If you have received this > > e-mail in error, please destroy this message and contact the > > Security Officer at PathGroup, Inc immediately at 615-562-9255. > > Thank you _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely > > for the named addressee(s) and contain confidential information. If > > you are not an addressee, or responsible for delivering this email > > to an addressee, you have received this email in error and are > > notified that reading, copying, or disclosing this email is > > prohibited. If you received this email in error, immediately reply > > to the sender and delete the message completely from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lpwenk <@t> sbcglobal.net Wed Nov 21 04:34:15 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Nov 21 04:34:16 2012 Subject: [Histonet] unsubscribing for Thanksgiving Message-ID: If you want to unsubscribe permanently , OR If you are going away for Thanksgiving and are setting your computer for ?I?m not here? (which all the Histonetters do not need to know), please unsubscribe. Go to the bottom of any Histonet email, from the computer you are receiving the Histonet emails. There are 2 links. Click on the link that has the words ?mailman/listinfo? towards the end. A new page pops up. Scroll to the bottom under ?Histonet Subscribers? Under ?unsubscribe?, type in your email address and hit unsubscribe. (That?s are far as I?m going, so I don?t know if there are any other instruction, but at least you are on the right page.) Go back to this page to re-subscribe when you want to (see right above ?unsubscribe?.). Peggy Wenk From DKBoyd <@t> chs.net Wed Nov 21 08:12:00 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Nov 21 08:12:16 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> Message-ID: <7EAFE982E328304DA6CE2B677BB762466E19A2A3@TN001WEXMBX12.US.chs.net> Thank you Bill, well stated! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, November 20, 2012 3:25 PM To: Bruce Gapinski; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pa Leeeze Like it or not, politics played a part in the cut of 88305. So did POLs, CAP and a host of other factors. Finger pointing in time of uncertainty somehow makes us all feel better, but it doesn't give us concrete ways of addressing the problem. Histology has enjoyed a fairly long period of great reimbursement, reasonable per-test costs, and a certain amount of security in that what we do is unique. That is all changing, but was likely to change at least some no matter who was elected to do whatever. Remember the panic when DRG's first arrived? There is no doubt that labs are going to have to get leaner, but this was already a trend. Find reasonable ways to cut costs. I know. We've been doing this for years.... But it needs to go further. Some people will lose their jobs. I may well be one of them and I don't like it, but it is a reality. If I go down, it will not be for lack of trying to maintain. 88305 cuts are big but there are a lot of clinical services getting cuts as well. Hospitals need to do what they can to keep the doors open for the benefit of the patient. Pay cuts, bonuses+/-, benefits, hiring freezes, capital freezes are all looming on the horizon. If at all possible, fight them, but do not exhaust yourselves. It's a new world - and it will sometimes be ugly. Blame the Democrats or the Republicans, Wall Street or Main Street, but figure out how to adapt. OK. So.... What can we do to ride out the storm? 1. Find a marketing advantage. POLs and certain smaller private labs cannot remain the "bargain" they once were. My lab is expectiing to get back some of what we lost to them a few years back. We are the only game in our town.... Why are we losing business to labs in other areas? It should all be staying here. 2. Become politically active. Demand better from your elected officials and from your professional organizations that are lobbyists(sp). If they can't do the job, use your vote or your membership fees to fire them OR run for office yourself. Become an activist in your professional organization. 3. Maintain high standards. Cut-backs and performance improvement need not automatically equate to less quality. I hate it when people assume that shaving a couple of minutes must necessitate poor cutting. How close to borderline is your current quality if this is your attitude. Yes, that was snarky, but think about it. 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T PANIC. When you are caught up in a panic mentality, thinking and problem solving suffer. We need our heads in the game if we are going to come out on top. (How's that for my best Zig Zigler impersonation)? Above all - have a nice day and thank you for letting me vent a bit. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Tuesday, November 20, 2012 10:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pa Leeeze Wow, How disappointing. Looking for constructive ways to keep my lab open and I get political stuff. Did you all go crazy in the 80's with Ronald Ray-gun and the DRG's? Too young? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From brian <@t> prometheushealthcare.com Wed Nov 21 09:35:36 2012 From: brian <@t> prometheushealthcare.com (brian@prometheushealthcare.com) Date: Wed Nov 21 09:36:57 2012 Subject: [Histonet] Happy Thanksgiving Message-ID: From all of us at Prometheus Healthcare - Have a great holiday! Brian Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 [1]brian@prometheushealthcare.com [2]www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** [3]http://twitter.com/PrometheusBlog References 1. 3D"mailto:brian@prometheushealthcare.com" 2. 3D"http://www.prometheushealthcare.com/" 3. 3D"http://twitter.com/PrometheusBlog" From BGapinski <@t> pathgroup.com Wed Nov 21 10:11:41 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Wed Nov 21 10:11:53 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: <7EAFE982E328304DA6CE2B677BB762466E19A2A3@TN001WEXMBX12.US.chs.net> References: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> <7EAFE982E328304DA6CE2B677BB762466E19A2A3@TN001WEXMBX12.US.chs.net> Message-ID: Dear Histonians, I am sorry. I wish I felt as you do. "We were looking at this for some time, and it was inevitable." "Histology has enjoyed a fairly long period of great reimbursement."" what we had been doing overall in healthcare could not be sustained"" The only thing that may cost a few jobs is if over-utilization is curbed." I don't get it. I don't run my lab that way. Or my Pathologists are real fat-cats who've been pulling the wool over my eyes for almost 40 years. How is it you all can post to HistoNet if you're so flippin lean? I don't have time to do this during work hours. I'M BUSY. Did you get rich in Histology? I didn't. Loosing a job is painful, and we in mid management loathe letting our staff go. The notion that I'm in panic mode is baiting at best. I asked for help from my colleagues and got a plate full of Rush Limbaugh. Untill HistoNet has CONSTRUCTIVE information, I will stay unsubscribed. Bruce Gapinski -----Original Message----- From: Boyd, Debbie M [mailto:DKBoyd@chs.net] Sent: Wednesday, November 21, 2012 6:12 AM To: O'Donnell, Bill; Bruce Gapinski; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pa Leeeze Thank you Bill, well stated! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, November 20, 2012 3:25 PM To: Bruce Gapinski; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pa Leeeze Like it or not, politics played a part in the cut of 88305. So did POLs, CAP and a host of other factors. Finger pointing in time of uncertainty somehow makes us all feel better, but it doesn't give us concrete ways of addressing the problem. Histology has enjoyed a fairly long period of great reimbursement, reasonable per-test costs, and a certain amount of security in that what we do is unique. That is all changing, but was likely to change at least some no matter who was elected to do whatever. Remember the panic when DRG's first arrived? There is no doubt that labs are going to have to get leaner, but this was already a trend. Find reasonable ways to cut costs. I know. We've been doing this for years.... But it needs to go further. Some people will lose their jobs. I may well be one of them and I don't like it, but it is a reality. If I go down, it will not be for lack of trying to maintain. 88305 cuts are big but there are a lot of clinical services getting cuts as well. Hospitals need to do what they can to keep the doors open for the benefit of the patient. Pay cuts, bonuses+/-, benefits, hiring freezes, capital freezes are all looming on the horizon. If at all possible, fight them, but do not exhaust yourselves. It's a new world - and it will sometimes be ugly. Blame the Democrats or the Republicans, Wall Street or Main Street, but figure out how to adapt. OK. So.... What can we do to ride out the storm? 1. Find a marketing advantage. POLs and certain smaller private labs cannot remain the "bargain" they once were. My lab is expectiing to get back some of what we lost to them a few years back. We are the only game in our town.... Why are we losing business to labs in other areas? It should all be staying here. 2. Become politically active. Demand better from your elected officials and from your professional organizations that are lobbyists(sp). If they can't do the job, use your vote or your membership fees to fire them OR run for office yourself. Become an activist in your professional organization. 3. Maintain high standards. Cut-backs and performance improvement need not automatically equate to less quality. I hate it when people assume that shaving a couple of minutes must necessitate poor cutting. How close to borderline is your current quality if this is your attitude. Yes, that was snarky, but think about it. 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T PANIC. When you are caught up in a panic mentality, thinking and problem solving suffer. We need our heads in the game if we are going to come out on top. (How's that for my best Zig Zigler impersonation)? Above all - have a nice day and thank you for letting me vent a bit. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Tuesday, November 20, 2012 10:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pa Leeeze Wow, How disappointing. Looking for constructive ways to keep my lab open and I get political stuff. Did you all go crazy in the 80's with Ronald Ray-gun and the DRG's? Too young? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From relia1 <@t> earthlink.net Wed Nov 21 11:11:06 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Nov 21 11:11:12 2012 Subject: [Histonet] RELIA Histology Careers Bulletin and a few Holiday Shopping Tips 11/21/2012 Message-ID: <009301cdc80b$32027630$96076290$@earthlink.net> Hi Histonetters!! I hope you are having a great day and gearing up for some holiday fun. The weather is getting cooler and the Holidays are right around the corner. I realize that at this time of year a job change is the furthest thing from most people's minds but I have some great opportunities that I wanted to share along with some great tips for getting some good deals while holiday shopping. All of my positions are full time permanent positions with premier companies who offer excellent salaries, benefits and relocation assistance. Most of them are willing to look at onsite interviews and start dates after the holidays so if you or anyone you know might be looking now or after the first of the year it wouldn't hurt to shoot me a quick e-mail at relia1@earthlink.net or give me a quick call toll free at 866-607-3542. Currently I have positions in NY, CO, TN, ME, PA, VA, OR and GA. Holiday shopping is always fun, crazy, chaotic, inspiring, and challenging. Here are some tips that might save you some time, money and stress. * If you have an android or iphone check out your app store for some amazing holiday shopping apps.! * If you want to get a head start on Black Friday check out the website www.bfads.net they have posted the Black Friday Sale ads from your favorite stores. They have a lot of the ads posted NOW that will be in your newspaper on Thanksgiving morning. * The Monday after Thanksgiving is known as Cyber Monday it is the busiest online shopping day of the year and there is another website that will give you heads up on deals for Cyber Monday. This website is www.cybermonday.com the best part of this site is that the stores that advertise on their site donate a percentage of sales to a scholarship fund. Again if you or anyone you know is interested in a new opportunity now or after the holidays get in touch with me. There are a lot of recruiters out there but remember I am the only one with the experience, connections and respect for you and your career that specializes exclusively in histology. I want to place you in a new position only if it is the right place, right time and right position for you. And I am always available for career advice, resume assistance or just a chat. Let me be your career advocate. Happy Thanksgiving!!! Pam 866-607-3542 Relia1@earthlink.net Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa <@t> yahoo.com Wed Nov 21 11:32:55 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 21 11:33:00 2012 Subject: [Histonet] RE: Help needed with mouse brain References: <3F0184241070BC41AD8C8EC2C45B50243A9933@pbrcas31.pbrc.edu> <3F0184241070BC41AD8C8EC2C45B50243A9935@pbrcas31.pbrc.edu> Message-ID: <1353519175.53543.YahooMailNeo@web163105.mail.bf1.yahoo.com> Mouse brain (and any other mouse tissue for that matter) are less fatty than most other tissues and require less processing times, specially in alcohols and mostly on xylene (if you are using it). The ideal solution for mouse tissues (and any other tissue for that matter) is using isopropyl alcohol and mineral oil. Ren? J. ________________________________ From: D'Andreas Williams To: histonet@lists.utsouthwestern.edu Cc: David Burk ; Shirley Ennis Sent: Tuesday, November 20, 2012 1:45 PM Subject: [Histonet] RE: Help needed with mouse brain Greetings, We are currently trying to optimize our processing protocols for mouse tissue on a VIP 6 processor. Of particular concern to us is mouse brain processing. For the mouse brain we have noticed "fraying" of the edge of the brain. The "fraying" looks a lot like? what we see when tissue is over processed. The brains cut as if they are overprocessed; they are brittle and crunch when they hit the blade. At times they roll up and create a hole in the ribbon as our histologists cuts them. Along with the "fraying" edges we noticed that the middle of the brain looks underprocessed. When the section is placed on the waterbath the section wrinkles greatly and spreads considerably, and if left longer than a few seconds explodes. We have tried several protocols, and we see this effect. The brains we are using are cut at about 4 mm thick, and they are sectioned at 5 microns at a temperature of 42 degrees. Below are links to 3 images of a brain. Image 1 shows the edge "fraying". Image 2 show the wrinkles in the tissue. The tissue was left as long as possible on the waterbath to remove wrinkles and avoid breaking apart. Image 3 shows the brain breaking apart before the wrinkles have had a chance to work their way out. Does anyone know what is causing this issue? Also, I have put together a document of all the protocols we have ran with images of the results that I can send to you. Thank you in advance. https://dl.dropbox.com/u/25427596/Histology/IMG_1682.JPG https://dl.dropbox.com/u/25427596/Histology/Wrinkles.jpg https://dl.dropbox.com/u/25427596/Histology/Center.jpg With Kindest Regards, D'Andreas Williams Research Associate Cell Biology & Bioimaging Core Pennington Biomedical Research Center dandreas.williams@pbrc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Nov 21 11:50:12 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Nov 21 11:50:24 2012 Subject: [Histonet] Pa Leeeze In-Reply-To: References: <4940DF6D1C5FDF48931B6966AAEF93958990DF@chimsx08.CHI.catholichealth.net> <7EAFE982E328304DA6CE2B677BB762466E19A2A3@TN001WEXMBX12.US.chs.net> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93958991B8@chimsx08.CHI.catholichealth.net> This post is Op-Ed in nature... So Be Cool. Should probably just let this thread die a quiet death over Thanksgiving, but I can't resist. Blame it on a short night and a very early morning ... or blame it on me. Whatever. My long and ranting post was also more of an Op-Ed. No person or persons were named nor intended to be personal. Sorry if it hit a nerve - but then that's what Op-Ed does. Is there a place for such on HistoNet? Maybe, maybe not. If asked how to retrieve an antigen - there may or may not be better ways of doing it but my opinions about it aren't worth much. But this 88305 issue, if you are in clinical work, is one that we are all dealing with without any particularly familiar ground to work with. To some degree opinions, one way or the other, is what we are left with. And, believe it or not, through all the griping, panic and befuddlement, I was able to gain useful insight. So, I vote that this is a legitimate forum for our concerns as well as our particular technical brilliance. Personally, I'm not all that smart. I'm just determined and focused. Information on the legislation is out there but it is far too laborious and nuanced for most of us poor scientists to completely comprehend. It wasn't written for us but for lawyers and insurance companies. I have to rely on people smarter than me to figure all of this stuff out. I like that HistoNet is full of smarter people. (A personal note: I actually did become very wealthy in histology. I just choose to drive a 2000 Mercury with a cracked windshield, broken door handles and a peculiar smell of hickory smoke and spoiled milk. I could air it out if the windows worked....call me eccentric - and a real smarta$$!) Bottom line: I've been jobless, it suc*ks. I've been without a home for a short duration, and it suc*ks even more. It will not happen to me again - even in this economy. Bustin' my chops to make sure it doesn't! My main tools are a cool head and a strong attitude and the occasional rant. (I am my own best tool) A lot of the rest of it is out of my sphere of control, but I am as forward thinking and as pro-active as I am able. So far, I'm still working, living indoors and still have heat and running water. I'm not going to let a cut in the money my company makes determine what I do or be my motivator. In fact, when I get home in the evening - I don't think about it one wit. I have a wife to love, a dog to pet, a dinner to eat and a bed to sleep in.... 88305 be dam*ned! And that gives me reason for a great Thanksgiving! I hope ya'll have a great one! - Shalom! Bill -----Original Message----- From: Bruce Gapinski [mailto:BGapinski@pathgroup.com] Sent: Wednesday, November 21, 2012 10:12 AM To: 'Boyd, Debbie M'; O'Donnell, Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pa Leeeze Dear Histonians, I am sorry. I wish I felt as you do. "We were looking at this for some time, and it was inevitable." "Histology has enjoyed a fairly long period of great reimbursement."" what we had been doing overall in healthcare could not be sustained"" The only thing that may cost a few jobs is if over-utilization is curbed." I don't get it. I don't run my lab that way. Or my Pathologists are real fat-cats who've been pulling the wool over my eyes for almost 40 years. How is it you all can post to HistoNet if you're so flippin lean? I don't have time to do this during work hours. I'M BUSY. Did you get rich in Histology? I didn't. Loosing a job is painful, and we in mid management loathe letting our staff go. The notion that I'm in panic mode is baiting at best. I asked for help from my colleagues and got a plate full of Rush Limbaugh. Untill HistoNet has CONSTRUCTIVE information, I will stay unsubscribed. Bruce Gapinski -----Original Message----- From: Boyd, Debbie M [mailto:DKBoyd@chs.net] Sent: Wednesday, November 21, 2012 6:12 AM To: O'Donnell, Bill; Bruce Gapinski; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pa Leeeze Thank you Bill, well stated! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, November 20, 2012 3:25 PM To: Bruce Gapinski; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pa Leeeze Like it or not, politics played a part in the cut of 88305. So did POLs, CAP and a host of other factors. Finger pointing in time of uncertainty somehow makes us all feel better, but it doesn't give us concrete ways of addressing the problem. Histology has enjoyed a fairly long period of great reimbursement, reasonable per-test costs, and a certain amount of security in that what we do is unique. That is all changing, but was likely to change at least some no matter who was elected to do whatever. Remember the panic when DRG's first arrived? There is no doubt that labs are going to have to get leaner, but this was already a trend. Find reasonable ways to cut costs. I know. We've been doing this for years.... But it needs to go further. Some people will lose their jobs. I may well be one of them and I don't like it, but it is a reality. If I go down, it will not be for lack of trying to maintain. 88305 cuts are big but there are a lot of clinical services getting cuts as well. Hospitals need to do what they can to keep the doors open for the benefit of the patient. Pay cuts, bonuses+/-, benefits, hiring freezes, capital freezes are all looming on the horizon. If at all possible, fight them, but do not exhaust yourselves. It's a new world - and it will sometimes be ugly. Blame the Democrats or the Republicans, Wall Street or Main Street, but figure out how to adapt. OK. So.... What can we do to ride out the storm? 1. Find a marketing advantage. POLs and certain smaller private labs cannot remain the "bargain" they once were. My lab is expectiing to get back some of what we lost to them a few years back. We are the only game in our town.... Why are we losing business to labs in other areas? It should all be staying here. 2. Become politically active. Demand better from your elected officials and from your professional organizations that are lobbyists(sp). If they can't do the job, use your vote or your membership fees to fire them OR run for office yourself. Become an activist in your professional organization. 3. Maintain high standards. Cut-backs and performance improvement need not automatically equate to less quality. I hate it when people assume that shaving a couple of minutes must necessitate poor cutting. How close to borderline is your current quality if this is your attitude. Yes, that was snarky, but think about it. 4. Remember the mantra of the Hitchhikers Guide to the Universe: DON'T PANIC. When you are caught up in a panic mentality, thinking and problem solving suffer. We need our heads in the game if we are going to come out on top. (How's that for my best Zig Zigler impersonation)? Above all - have a nice day and thank you for letting me vent a bit. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Tuesday, November 20, 2012 10:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pa Leeeze Wow, How disappointing. Looking for constructive ways to keep my lab open and I get political stuff. Did you all go crazy in the 80's with Ronald Ray-gun and the DRG's? Too young? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From LBUSTAMANTE <@t> cvm.tamu.edu Wed Nov 21 11:55:36 2012 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Wed Nov 21 11:55:43 2012 Subject: [Histonet] Microwave processing. ..... Isopropanol Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC97C4650D2@CVMMB02.cvm.tamu.edu> If your lab does Microwave processing using Isopropanol. How do you disposed this reagent? Thank you. Lin From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 21 12:04:59 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 21 12:05:45 2012 Subject: [Histonet] RE: Microwave processing. ..... Isopropanol In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC97C4650D2@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC97C4650D2@CVMMB02.cvm.tamu.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF02D823@ex07.net.ucsf.edu> We put it in a carboy with all other solvents (ethanol, xylene, clear-rite, plus formalin) and waste management picks it up. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, November 21, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing. ..... Isopropanol If your lab does Microwave processing using Isopropanol. How do you disposed this reagent? Thank you. Lin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Cline <@t> meritushealth.com Wed Nov 21 12:04:00 2012 From: Joyce.Cline <@t> meritushealth.com (Joyce Cline) Date: Wed Nov 21 12:09:44 2012 Subject: [Histonet] RE: Microwave processing. ..... Isopropanol In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC97C4650D2@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC97C4650D2@CVMMB02.cvm.tamu.edu> Message-ID: <4A187D87076BF348A9A9FBAD6A6659BC198982FDB2@MHXCHCM.wchsys.org> We dispose of our flammables by a waste disposal company. Joyce Cline, H. T. (ASCP) Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@meritushealth.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin [LBUSTAMANTE@cvm.tamu.edu] Sent: Wednesday, November 21, 2012 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing. ..... Isopropanol If your lab does Microwave processing using Isopropanol. How do you disposed this reagent? Thank you. Lin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From cpyse <@t> x-celllab.com Wed Nov 21 12:43:10 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Nov 21 12:43:22 2012 Subject: [Histonet] Tissue Processors In-Reply-To: <52EFCA115E89304590180DA96959860944EEB4AE16@OC1EX202.ME.MBGOV.CA> References: <52EFCA115E89304590180DA96959860944EEB4AE16@OC1EX202.ME.MBGOV.CA> Message-ID: <002701cdc818$0f75a170$2e60e450$@com> Rhonda I have both a Thermo-Fisher Excelsior and a Sakura VIP6. Both work well but I prefer the Excelsior. There is less hands on time for changing reagents, flushing and cleaning. I did have problems at first with the VIP. Since Sakura replaced the gate valve all has been great. If I need to replace either machine I would purchase the Excelsior. This is just my opinion, I'm sure other out there may disagree with me. Everyone out there in Histoland have a safe, healthy Thanksgiving. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, November 20, 2012 5:32 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processors Does anyone have the Thermo Shandon Excelsior or the Leica ASP300 S tissue processors? What do you like or not like about the one you have? Thanks Rhonda Gregoire, R.T. Charge Technologist Clinical Pathology/TSE Section Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Initiatives 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Nov 23 08:20:48 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Nov 23 08:21:08 2012 Subject: [Histonet] plant histology Message-ID: All, Does anyone have a basic processing schedule for plant histology? Thanks, Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartlett@cdc.hhs.gov From CThornton <@t> dahlchase.com Mon Nov 26 08:41:33 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Nov 26 08:42:35 2012 Subject: [Histonet] Ultra users Message-ID: Has anyone encountered incorrect/inappropriate staining using the Ultra? Twice we have had slides that looked like they had the wrong antibody applied, although the slides and dispensers were labeled correctly (once with TTF-1, once with p16; both had membranous staining). I was wondering if where the reagent wheel both spins and moves on the arm, occasionally it doesn't quite make it to the dispenser it is supposed to and applies the wrong one. Does anyone have any experience/ideas as to why this might happen? thank you! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From CThornton <@t> dahlchase.com Mon Nov 26 08:53:11 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Nov 26 08:54:12 2012 Subject: [Histonet] GMS fungus control Message-ID: Second question of the day: what is everyone using for their fungus control for GMS? We have always used pneumocystis when looking for pneumo, and Aspergillus when looking for fungus, but wondering if anyone uses any type of fungus just to see that stain worked properly. We are having difficulty locating tissue for both pneumo and Aspergillus, and are trying to avoid buying commercial QC slides. thanks again! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From gu.lang <@t> gmx.at Mon Nov 26 09:21:12 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Nov 26 09:21:23 2012 Subject: AW: [Histonet] Ultra users In-Reply-To: References: Message-ID: <001f01cdcbe9$ac5d8140$051883c0$@gmx.at> Clare, last month we had recurring troubles with the Ultra. With a couple of runs the slides were not stained correctly. In one run we had slides with no colour, with dab-colour, with purple-hematoxylin color, with specific antibody-staining and without. The whole panorama. Some runs were completed without any alarm. Some stopped with the alarm that's due to the fact, that the arm didn't find the right position at the end of its turn. This error was told to be caused by an invalid sensor, that is humpered by too much moisture (fog) in the ultra. Ventana said the slides are the culprit (like every time). We took they recommended from Leica. And had the same troubles. After that no one really had an idea. Now we've got a new Ultra. Tomorrow I know more, if it was the instrument or the reagens. Our Ultra is now about two years old and has been running quite 24 hours a day.... I also have the suspiscion, that the spinning arm and the hammer are the cause. Please tell me more about your troubles. And how you fixed it. Kind regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Clare Thornton Gesendet: Montag, 26. November 2012 15:42 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ultra users Has anyone encountered incorrect/inappropriate staining using the Ultra? Twice we have had slides that looked like they had the wrong antibody applied, although the slides and dispensers were labeled correctly (once with TTF-1, once with p16; both had membranous staining). I was wondering if where the reagent wheel both spins and moves on the arm, occasionally it doesn't quite make it to the dispenser it is supposed to and applies the wrong one. Does anyone have any experience/ideas as to why this might happen? thank you! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Mon Nov 26 09:47:31 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Nov 26 10:51:59 2012 Subject: [Histonet] Wrinkle Out Message-ID: Good morning! Is anybody using this product, Wrinkle Out Water Bath H@O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. Thanks! Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From joanne0658 <@t> comcast.net Mon Nov 26 11:08:20 2012 From: joanne0658 <@t> comcast.net (Joanne) Date: Mon Nov 26 11:08:18 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: 1353379755.37709.YahooMailNeo@web121503.mail.ne1.yahoo.com Message-ID: <98F830AFB4DC4EBBBDE5FC1CD93DFD7A@JoannePC> Mr. Buesa . . .You're always a voice of reason. Thank you!!!!!! From rjbuesa <@t> yahoo.com Mon Nov 26 12:13:49 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 26 12:13:53 2012 Subject: [Histonet] Wrinkle Out In-Reply-To: References: Message-ID: <1353953629.4956.YahooMailNeo@web163104.mail.bf1.yahoo.com> Avoid all those dangers and just add any liquid soap at 0.5% in your water bath with the same effects Ren? J. From: "Grantham, Andrea L - (algranth)" To: HISTONET Sent: Monday, November 26, 2012 10:47 AM Subject: [Histonet] Wrinkle Out Good morning! Is anybody using this product, Wrinkle Out Water Bath H@O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. Thanks! Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akelley1 <@t> slu.edu Mon Nov 26 12:18:52 2012 From: akelley1 <@t> slu.edu (Amanda Kelley) Date: Mon Nov 26 12:19:04 2012 Subject: [Histonet] Wrinkle Out In-Reply-To: <1353953629.4956.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <1353953629.4956.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: Rene, Does the soap affect IHC or ISH stains? Amanda On Mon, Nov 26, 2012 at 12:13 PM, Rene J Buesa wrote: > Avoid all those dangers and just add any liquid soap at 0.5% in your water > bath with the same effects > Ren? J. > > From: "Grantham, Andrea L - (algranth)" > To: HISTONET > Sent: Monday, November 26, 2012 10:47 AM > Subject: [Histonet] Wrinkle Out > > Good morning! > Is anybody using this product, Wrinkle Out Water Bath H@O, in their > waterbaths and what precautions are you taking if so? > The label states that the product is highly toxic, a hepatoxin. > I received a sample of it and wasn't aware of its toxicity before I > requested the sample at the NSH S/C. > > Thanks! > > Andi Grantham > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akelley1@slu.edu DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. From cheastys <@t> svm.vetmed.wisc.edu Mon Nov 26 12:19:23 2012 From: cheastys <@t> svm.vetmed.wisc.edu (Sandy's Mail) Date: Mon Nov 26 12:19:26 2012 Subject: [Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation? Message-ID: Hello all, We were using (with great success) the Max Vision "Max Poly-Two" with our mostly DAKO antibodies on our Lab Vision autostainer. Mostly canine and feline tissues, mostly tumor markers. We were so happy. Better quality, great specificity, great reproducibility, and half the price of the Lab Vision polymer detection kit. Now, Max Vision is discontinuing their IHC line, and we are filled with woe and wringing our collective histology hands. Any recommendations for a quality rabbit/mouse polymer detection kit would be most appreciated. Thank you, Sandy From gayle.callis <@t> bresnan.net Mon Nov 26 12:38:23 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Nov 26 12:38:31 2012 Subject: [Histonet] RE: Wrinkle Out Water Bath Message-ID: <000101cdcc05$3927b9e0$ab772da0$@bresnan.net> You wrote: Is anybody using this product, Wrinkle Out Water Bath H <@t> O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. **************************************************************************** ************************ In reading the MSDS for this product, all it contains is 1% isopropyl, 18% Ethanol, and 1% methanol citing possible damage to liver. Why not avoid spending the money and just use 20% ethanol in your water bath according to how this product is used. It will work just as well as this. You could even make up your own mixture with isopropyl and ethanol, forget the more toxic and expensive methanol. These alcohols are all used for tissue processing e.g. denatured reagent alcohols, isopropyl, ethanol and/or methanol alone, and just as toxic to the liver. Beware! Too high of an alcohol concentration can cause your sections to explode on a warm water bath. We never used alcohol in warm water bath to flatten tissue sections. Instead, we used RT 10% ethyl alcohol in a glass staining dish, floated a section on this, picked up section onto a slide then went to a warm water bath, gently lowered the section but kept top paraffin portion of section on the slide, and watch the section flatten in the warm water. This prevented a section from exploding wildly or losing the section on the water bath. All the alcohol or a detergent does is reduce the surface tension of the water so the section flattens. Tween 20 has been used too. I don't think any of these damage IHC since the antigens are still protected by the paraffin in the tissue section. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT From gayle.callis <@t> bresnan.net Mon Nov 26 12:41:26 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Nov 26 12:41:34 2012 Subject: [Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation? Message-ID: <000601cdcc05$a63c12b0$f2b43810$@bresnan.net> Biocare and Golden Bridge International have kits. I believe Vector can be added to this group too. Gayle Callis HTL/HT/MT(ASCP) From rsrichmond <@t> gmail.com Mon Nov 26 12:48:38 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Nov 26 12:48:42 2012 Subject: [Histonet] Re: GMS fungus control Message-ID: Clare J. Thornton, HTL(ASCP)QIHC at Dahl-Chase Diagnostic Services, Bangor, Maine asks: >>What is everyone using for their fungus control for GMS? We have always used pneumocystis when looking for pneumocystis, and Aspergillus when looking for fungus, but wondering if anyone uses any type of fungus just to see that stain worked properly. We are having difficulty locating tissue for both pneumo and Aspergillus, and are trying to avoid buying commercial QC slides.<< It's preferable to have a pneumocystis control when needed. Histoplasma is the best fungus control, when you can get it, because it's the most difficult to stain. In ordinary surgical material, aspergillus is most often seen in paranasal sinus material. Bob Richmond Samurai Pathologist Maryville TN From BGapinski <@t> pathgroup.com Mon Nov 26 12:50:05 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Mon Nov 26 12:50:16 2012 Subject: [Histonet] Ultra Message-ID: I too have had problems with my Ultra. I am so glad I place a control at the top of every slide. Sometimes no Hematoxylin, sometimes no positive staining. I've documented half a dozen cases. One thing we noticed is that the antibody vials get plugged up from the protein in the antibody. We inspect every vial before we place it on the instrument. The other thing we look for is antibody in the spout of the vial. It will recede. Called Ventana, and they said " There are plenty of extra drops in the vial, so prime the vial. Bad advise, here's why. The instrument has no idea how many drops are disposed of during priming. So we ended up with another bunch of primaries with no drops left. Now we prime without expelling any reagent and how that works better. With this problem I feel Ventana owes us URA (Ultimate Reagent Access) then we can prime as we go. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From claycal44 <@t> yahoo.com Mon Nov 26 14:09:39 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Mon Nov 26 14:09:43 2012 Subject: [Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation? In-Reply-To: References: Message-ID: <1353960579.45778.YahooMailNeo@web125603.mail.ne1.yahoo.com> From: Sandy's Mail To: histonet@lists.utsouthwestern.edu Sent: Monday, November 26, 2012 10:19 AM Subject: [Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation? Hello all, ? ? ? ? ? ? ? ? We were using (with great success) the Max Vision "Max Poly-Two" with our mostly DAKO antibodies on our Lab Vision autostainer. Mostly canine and feline tissues, mostly tumor markers. We were so happy. Better quality, great specificity, great reproducibility, and half the price of the Lab Vision polymer detection kit. Now, Max Vision is discontinuing their IHC line, and we are filled with woe and wringing our collective histology hands. ? ? ? ? ? ? ? ? Any recommendations for a quality rabbit/mouse polymer detection kit would be most appreciated. Thank you, Sandy Biocare Medical has one. Nancy Lowen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dhewitt <@t> hvhs.org Mon Nov 26 15:46:47 2012 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Mon Nov 26 15:46:52 2012 Subject: [Histonet] Ultra In-Reply-To: References: Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B016942B3@MX-HVB-02.hvhs.org> We have the same issue from time to time on our Ultra, our vortex mixers seem to clog after about 3-4 months of use. The engineer comes in, unclogs them and everything is good for another 3-4 months. They have never really been able to figure it out why they clog but, but thankfully the service is still covered, just a large nuisance. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Monday, November 26, 2012 1:50 PM To: 'cthornton@dahlchase.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ultra I too have had problems with my Ultra. I am so glad I place a control at the top of every slide. Sometimes no Hematoxylin, sometimes no positive staining. I've documented half a dozen cases. One thing we noticed is that the antibody vials get plugged up from the protein in the antibody. We inspect every vial before we place it on the instrument. The other thing we look for is antibody in the spout of the vial. It will recede. Called Ventana, and they said " There are plenty of extra drops in the vial, so prime the vial. Bad advise, here's why. The instrument has no idea how many drops are disposed of during priming. So we ended up with another bunch of primaries with no drops left. Now we prime without expelling any reagent and how that works better. With this problem I feel Ventana owes us URA (Ultimate Reagent Access) then we can prime as we go. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Tue Nov 27 07:01:55 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Nov 27 07:02:04 2012 Subject: [Histonet] Manager of Medical and Scientific Affairs - Cancer Diagnostics Message-ID: <29ba8c84fda2e9a96d128cf4f33d9138@mail.gmail.com> Good morning Histonet, One of our retained clients who is a global leader in histology and IHC is searching for a Manager of Medical and Scientific Affairs to join their team. The ideal candidate will have an Medical Degree (required) and at least 2 years of practice or hospital experience in Anatomical Pathology. This is a very visible and high level position within this organization and will offer a strong career track and package. If you or anyone you may know would be interested in having a conversation to learn more, please contact me at mw@personifysearch.com or call me at 800.875.6188 ext. 103. Thanks and have a wonderful Tuesday! Matt Ward Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From portera <@t> msu.edu Tue Nov 27 08:24:12 2012 From: portera <@t> msu.edu (Amy Porter) Date: Tue Nov 27 08:23:58 2012 Subject: [Histonet] Looking for US Distributor for biological dyes Message-ID: <002701cdccaa$df875f70$9e961e50$@edu> Does anyone know of a U.S. distributor that carries Chroma-Gesellschaft dyes??? Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 portera@msu.edu www.humanpathology.msu.edu From rjbuesa <@t> yahoo.com Tue Nov 27 09:18:44 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 27 09:18:53 2012 Subject: [Histonet] Looking for US Distributor for biological dyes In-Reply-To: <002701cdccaa$df875f70$9e961e50$@edu> References: <002701cdccaa$df875f70$9e961e50$@edu> Message-ID: <1354029524.20655.YahooMailNeo@web163105.mail.bf1.yahoo.com> Try contacting Aldrich Co. Ren? J. From: Amy Porter To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 27, 2012 9:24 AM Subject: [Histonet] Looking for US Distributor for biological dyes Does anyone know of a U.S. distributor that carries Chroma-Gesellschaft dyes??? Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI? 48824-3320 Phone:? 517-884-5026 Fax:? 517-432-1368 portera@msu.edu http://www.humanpathology.msu.edu/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Nov 27 09:29:06 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Nov 27 09:29:15 2012 Subject: [Histonet] Job Opening Message-ID: <1354030146.34997.YahooMailNeo@web5713.biz.mail.ne1.yahoo.com> Hi, I am posting this for a friend. ?https://www1.recruitingcenter.net/Clients/MRIGlobal/PublicJobs/controller.cfm?jbaction=JobProfile&Job_Id=10951&esid=az ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From gayle.callis <@t> bresnan.net Tue Nov 27 10:20:57 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Nov 27 10:21:09 2012 Subject: [Histonet] Looking for US Distributor for biological dyes In-Reply-To: <002701cdccaa$df875f70$9e961e50$@edu> References: <002701cdccaa$df875f70$9e961e50$@edu> Message-ID: <001501cdccbb$307b4cb0$9171e610$@bresnan.net> Amy, Cellpoint Scientific. You have to call them for information on what you want, or a catalog? Telephone: 800-999-9734 or email: info@cellpointscientific.com They also have brain matrix devices for rodents, etc., but no website other than contact information. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Tuesday, November 27, 2012 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for US Distributor for biological dyes Does anyone know of a U.S. distributor that carries Chroma-Gesellschaft dyes??? Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 portera@msu.edu www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Tue Nov 27 10:42:23 2012 From: portera <@t> msu.edu (Amy Porter) Date: Tue Nov 27 10:42:14 2012 Subject: [Histonet] Looking for US Distributor for biological dyes In-Reply-To: <001501cdccbb$307b4cb0$9171e610$@bresnan.net> References: <002701cdccaa$df875f70$9e961e50$@edu> <001501cdccbb$307b4cb0$9171e610$@bresnan.net> Message-ID: <005701cdccbe$2d3716d0$87a54470$@edu> Cell Point has stopped carrying various dyes based on low usage. This was my primary vendor.... :-( Looking for good Luxol Fast Blue -----Original Message----- From: gayle callis [mailto:gayle.callis@bresnan.net] Sent: Tuesday, November 27, 2012 11:21 AM To: 'Amy Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Looking for US Distributor for biological dyes Amy, Cellpoint Scientific. You have to call them for information on what you want, or a catalog? Telephone: 800-999-9734 or email: info@cellpointscientific.com They also have brain matrix devices for rodents, etc., but no website other than contact information. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Tuesday, November 27, 2012 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for US Distributor for biological dyes Does anyone know of a U.S. distributor that carries Chroma-Gesellschaft dyes??? Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 portera@msu.edu www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Nov 27 12:31:41 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Nov 27 12:31:47 2012 Subject: [Histonet] Re: Wrinkle Out Message-ID: <9F3CFEE76E51B64991C7485270890B400CE1164A@EX4.lj.gnf.org> We always put a small (tiny!) drop of Kodak PhotoFlo in the water bath. It's a surfactant, so likely any diluted detergent would work. Be careful not to get too much in there, your ribbons will want to sink. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From Elizabeth.Cameron <@t> jax.org Tue Nov 27 12:35:12 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Tue Nov 27 12:35:23 2012 Subject: [Histonet] Uneven Staining Message-ID: About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Barry.R.Rittman <@t> uth.tmc.edu Tue Nov 27 12:45:56 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Nov 27 12:46:04 2012 Subject: [Histonet] RE: Uneven Staining In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDDBC@UTHCMS1.uthouston.edu> Elizabeth as the sections are evenly stained but vary in their staining from slide to slide, one suggestion is that section thickness varies. I know that this is reaching but if you section a ribbon and then place this on the water bath and then cut the next portion of ribbon then perhaps the microtome has a problem with the pause between cutting. If this is the case try cutting a long ribbon and then mounting the separate slides. Again, know this is reaching but difficult to see what it could be. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron [Elizabeth.Cameron@jax.org] Sent: Tuesday, November 27, 2012 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uneven Staining About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elizabeth.Cameron <@t> jax.org Tue Nov 27 13:12:53 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Tue Nov 27 13:13:03 2012 Subject: [Histonet] RE: Uneven Staining In-Reply-To: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDDBC@UTHCMS1.uthouston.edu> References: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDDBC@UTHCMS1.uthouston.edu> Message-ID: Barry, Thanks for the suggestion. We are generally only able to get about 10-15 sections in a ribbon due to the nature of the tissue, and the pale staining last for 30+ sections at times. We are also cutting on multiple microtomes (one microtome/kidney, but MANY kidneys!), so all microtomes would have to be affected. No ideas on this one, so I appreciate any far-reaching thoughts! Thanks, Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, November 27, 2012 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Elizabeth as the sections are evenly stained but vary in their staining from slide to slide, one suggestion is that section thickness varies. I know that this is reaching but if you section a ribbon and then place this on the water bath and then cut the next portion of ribbon then perhaps the microtome has a problem with the pause between cutting. If this is the case try cutting a long ribbon and then mounting the separate slides. Again, know this is reaching but difficult to see what it could be. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron [Elizabeth.Cameron@jax.org] Sent: Tuesday, November 27, 2012 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uneven Staining About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From rjbuesa <@t> yahoo.com Tue Nov 27 14:24:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 27 14:24:55 2012 Subject: [Histonet] Uneven Staining In-Reply-To: References: Message-ID: <1354047891.71482.YahooMailNeo@web163105.mail.bf1.yahoo.com> This is quite a puzzling situation. You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of "diluting" in water. Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. Ren? J. From: Elizabeth Cameron To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 27, 2012 1:35 PM Subject: [Histonet] Uneven Staining About a year ago, I posted about issues with staining mouse kidney sections (see below for original post).? We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale.? This time it seems to be consistent across the slide, so I know it is not a drying issue.? They are serial sections, so they are all cut together and stained together, and they are from the same animal.? I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference.? We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining.? I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath.? They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling.? Has anyone experienced anything like this?? Any other ideas?? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys.? We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining.? Half of a section will stain as it should, and the rest of the section is very pale.? It seems to be in a similar area from one section to the next, but not exactly the same area.? It does not look like a deparaffinization issue.? There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine.? The sections are 6 microns. Any ideas on why this might be happening?? Thanks! > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> gmail.com Tue Nov 27 15:01:06 2012 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Tue Nov 27 15:01:24 2012 Subject: [Histonet] Uneven Staining In-Reply-To: <1354047891.71482.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <1354047891.71482.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <04F81A78-35D7-4D7E-A0DA-36AB880F2B11@gmail.com> As most other bases have been covered, do you leave space between the slides in the rack? Once in a blue moon we had stains from the same rack that differed in intensity, after much troubleshooting we started putting space between the slides and we haven't had the problem since. Just an idea. Cristi Sent from my iPhone On Nov 27, 2012, at 12:24 PM, Rene J Buesa wrote: > This is quite a puzzling situation. > You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of "diluting" in water. > Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. > Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. > Ren? J. > > From: Elizabeth Cameron > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, November 27, 2012 1:35 PM > Subject: [Histonet] Uneven Staining > > About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for > hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! > Thanks, > Liz > > We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Tue Nov 27 16:13:32 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Tue Nov 27 16:13:53 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 11/27/2012 and will not return until 12/04/2012. Note: For Cytology issues, please call Robin (day) at 8-421-5040, Wanda (day) 8-421-5426, or Eric (swing) 8-421-5405 For Histology issues, please call Mario (day) 8-421-4961, Barbara (swing) 8-421-4959, Bob (IHC) 8-421-4706, Kiran at 8-421-5404, or general histology client service at 8-421-5408 or Lotus Notes. From amber.mckenzie <@t> gastrodocs.net Wed Nov 28 00:16:10 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Nov 28 00:13:24 2012 Subject: [Histonet] Waste drum labeling Message-ID: <5A33C952BB67F4468AF1F36D739212BC7B5CD4FD@JERRY.Gia.com> For EPA standards, how do you label your waste drums? I have one 55 lb drum that we only pour ventana waste in. What numbers do you write in the diamond to cover all the kits that you stain with? 2 reps from EPA came by my lab yesterday and said i needed an emergency phone list by our phone?? Does anyone have the checklist for EPA that small labs should follow? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cristi Rigazio [cls71877@gmail.com] Sent: Tuesday, November 27, 2012 3:01 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Elizabeth Cameron Subject: Re: [Histonet] Uneven Staining As most other bases have been covered, do you leave space between the slides in the rack? Once in a blue moon we had stains from the same rack that differed in intensity, after much troubleshooting we started putting space between the slides and we haven't had the problem since. Just an idea. Cristi Sent from my iPhone On Nov 27, 2012, at 12:24 PM, Rene J Buesa wrote: > This is quite a puzzling situation. > You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of "diluting" in water. > Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. > Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. > Ren? J. > > From: Elizabeth Cameron > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, November 27, 2012 1:35 PM > Subject: [Histonet] Uneven Staining > > About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for > hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! > Thanks, > Liz > > We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d.a.faichney <@t> stir.ac.uk Wed Nov 28 04:31:49 2012 From: d.a.faichney <@t> stir.ac.uk (Debbie Faichney) Date: Wed Nov 28 04:32:29 2012 Subject: [Histonet] RE: Uneven Staining In-Reply-To: References: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDDBC@UTHCMS1.uthouston.edu> Message-ID: <8ED3F2CA5B78E142B8193376C57330F8FA2F3054BA@EXCH2007.ad.stir.ac.uk> Liz, Could it be due to the presence of residual wash water or previous solution diluting the stain to give patchiness? On the same thread, insufficient agitation of the slides in the solutions? You do not say if staining is by hand or automated as this may be a contributor. Whilst I have no experience of the staining method in use, I stain by hand and therefore able to ensure the previous solution is well drained prior to placing in the next. The slides are also dipped in and out( once or twice) to ensure the previous solution is washed off as well as allowing the stain to be evenly distributed amongst the slides. I hope you are able to resolve this frustrating issue. Kind regards Debbie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: 27 November 2012 19:13 To: Rittman, Barry R; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Barry, Thanks for the suggestion. We are generally only able to get about 10-15 sections in a ribbon due to the nature of the tissue, and the pale staining last for 30+ sections at times. We are also cutting on multiple microtomes (one microtome/kidney, but MANY kidneys!), so all microtomes would have to be affected. No ideas on this one, so I appreciate any far-reaching thoughts! Thanks, Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, November 27, 2012 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Elizabeth as the sections are evenly stained but vary in their staining from slide to slide, one suggestion is that section thickness varies. I know that this is reaching but if you section a ribbon and then place this on the water bath and then cut the next portion of ribbon then perhaps the microtome has a problem with the pause between cutting. If this is the case try cutting a long ribbon and then mounting the separate slides. Again, know this is reaching but difficult to see what it could be. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron [Elizabeth.Cameron@jax.org] Sent: Tuesday, November 27, 2012 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uneven Staining About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is ranked in the top 50 in the world in The Times Higher Education 100 Under 50 table, which ranks the world's best 100 universities under 50 years old. The University of Stirling is a charity registered in Scotland, number SC 011159. From LPaveli1 <@t> hurleymc.com Wed Nov 28 06:22:24 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Nov 28 06:22:31 2012 Subject: [Histonet] RE: Uneven Staining In-Reply-To: <8ED3F2CA5B78E142B8193376C57330F8FA2F3054BA@EXCH2007.ad.stir.ac.uk> References: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDDBC@UTHCMS1.uthouston.edu> , <8ED3F2CA5B78E142B8193376C57330F8FA2F3054BA@EXCH2007.ad.stir.ac.uk> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56B42C@EXCHANGEMB1.hmc.hurleymc.com> I have had this problem on a GI recut!! The first time through, the staining was perfect. The pathologist requested recuts, and I was cutting surgicals, so I put it in the back of the line up on my already melting ice bath to cut later. The recut was terrible. Nuclear staining was very weak! The block soaked up too much water. BUT!! The ribbon got progressively better on the slide!! Now, this lead to another discovery. In lovely processing.....especially with a small 2mm GI biopsy, it shouldn't matter how long it sat on the wet ice, right??! Now I'm thinking it is a processing issue, NOT my wet ice, or how long I let it soak......right?!! In lovely processing land (i.e. research!!), if the tissue was processed well, and wax was infiltrated properly, it wouldn't soak up water. Don't you think THAT is the problem, histo friends?? At first, I thought.....oh....it's the dewaxing, and then I thought it was something not tight or too tight on the microtome, but you said everyone has the same problem. Are they all the same type of microtomes, if not, then I'm leaning towards processing issues........similar to my GI issue. Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Debbie Faichney [d.a.faichney@stir.ac.uk] Sent: Wednesday, November 28, 2012 5:31 AM To: Elizabeth Cameron; Rittman, Barry R; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Liz, Could it be due to the presence of residual wash water or previous solution diluting the stain to give patchiness? On the same thread, insufficient agitation of the slides in the solutions? You do not say if staining is by hand or automated as this may be a contributor. Whilst I have no experience of the staining method in use, I stain by hand and therefore able to ensure the previous solution is well drained prior to placing in the next. The slides are also dipped in and out( once or twice) to ensure the previous solution is washed off as well as allowing the stain to be evenly distributed amongst the slides. I hope you are able to resolve this frustrating issue. Kind regards Debbie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: 27 November 2012 19:13 To: Rittman, Barry R; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Barry, Thanks for the suggestion. We are generally only able to get about 10-15 sections in a ribbon due to the nature of the tissue, and the pale staining last for 30+ sections at times. We are also cutting on multiple microtomes (one microtome/kidney, but MANY kidneys!), so all microtomes would have to be affected. No ideas on this one, so I appreciate any far-reaching thoughts! Thanks, Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, November 27, 2012 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Elizabeth as the sections are evenly stained but vary in their staining from slide to slide, one suggestion is that section thickness varies. I know that this is reaching but if you section a ribbon and then place this on the water bath and then cut the next portion of ribbon then perhaps the microtome has a problem with the pause between cutting. If this is the case try cutting a long ribbon and then mounting the separate slides. Again, know this is reaching but difficult to see what it could be. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron [Elizabeth.Cameron@jax.org] Sent: Tuesday, November 27, 2012 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uneven Staining About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is ranked in the top 50 in the world in The Times Higher Education 100 Under 50 table, which ranks the world's best 100 universities under 50 years old. The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Nov 28 06:52:11 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Nov 28 06:52:20 2012 Subject: [Histonet] Waste drum labeling In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC7B5CD4FD@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC7B5CD4FD@JERRY.Gia.com> Message-ID: <001501cdcd67$2f55a540$8e00efc0$@com> I would also be interested in the answers to this. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 28, 2012 1:16 AM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste drum labeling For EPA standards, how do you label your waste drums? I have one 55 lb drum that we only pour ventana waste in. What numbers do you write in the diamond to cover all the kits that you stain with? 2 reps from EPA came by my lab yesterday and said i needed an emergency phone list by our phone?? Does anyone have the checklist for EPA that small labs should follow? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cristi Rigazio [cls71877@gmail.com] Sent: Tuesday, November 27, 2012 3:01 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Elizabeth Cameron Subject: Re: [Histonet] Uneven Staining As most other bases have been covered, do you leave space between the slides in the rack? Once in a blue moon we had stains from the same rack that differed in intensity, after much troubleshooting we started putting space between the slides and we haven't had the problem since. Just an idea. Cristi Sent from my iPhone On Nov 27, 2012, at 12:24 PM, Rene J Buesa wrote: > This is quite a puzzling situation. > You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of "diluting" in water. > Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. > Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. > Ren? J. > > From: Elizabeth Cameron > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, November 27, 2012 1:35 PM > Subject: [Histonet] Uneven Staining > > About a year ago, I posted about issues with staining mouse kidney > sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! > Thanks, > Liz > > We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Nov 28 06:54:55 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Nov 28 06:55:02 2012 Subject: [Histonet] eosin in the processor Message-ID: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> Hi Everyone, Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues. ?I can't remember which station we put it in (I think it was the 2nd 100% ethanol). ?Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure). Thanks, Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From mw <@t> personifysearch.com Wed Nov 28 06:58:19 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Nov 28 06:58:28 2012 Subject: [Histonet] Workflow Consulting Opportunity Message-ID: <6fcc98457b646c56f2175f1cf3026f32@mail.gmail.com> Good morning, We have had a new position open with a global leader in histology. Please contact me directly at mw@personifysearch.com to learn more. Manager, Workflow Consulting ?East The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Cancer Diagnostics, Anatomical Pathology, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. The Opportunity: The company currently has an opening for an Manager, Workflow Consulting in Cancer Diagnostics. The person who fills this position can live anywhere in the Eastern US. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: Commensurate with Experience + Bonus Primary Responsibilities: The primary responsibility of this role will be to achieve Company sales and profitability goals by offering a value-added service to end-user that provides workflow consulting services and lean principles to optimize customer laboratories performance. Drive change in anatomic pathology laboratories utilizing Lean principles, information management, and hardware/software solutions. The solution for such a change, efficiency gains and waste elimination is the Company product offering. Additional Responsibilities: - Achieve monthly, quarterly, annual unit and revenue goals for the Division. Track KPI to measure revenue generated through Lean Consulting Services - Analyze new and existing customer laboratory organizations and workflow practices and recommend short and long-term improvements - Utilize Company Business System tools to credibly recommend changes to lab practices to eliminate waste, reduce cost and improve quality and turnaround times - Analyze and report market trends and innovative competitive activities for Lean Services - Working in conjunction with local sales representatives, Area Sales Managers and Directors of Sales plan and schedule face-face account calls to current and potential end-users. Train Sales force on basic lean principles for them to help market lean services and be able to follow-up with customers - In conjunction with Sales and Marketing, identify and develop key accounts in the territory. In conjunction with Director of Corporate Accounts, manage national accounts within territory requiring corporate coordination to enable closure and compliance of contracts - Prepare monthly territory status reports on lean activities to Management Maintain and report monthly on Workflow opportunities and projects - Manage operating expenses within assigned budget - Maintain technical, product, applications and sales skill knowledge. Maintain current knowledge of competition and market through study of competitive marketing information, competitive literature, and field surveillance or competition - Prospect for all product opportunities. Follow-up on all sales leads with status review immediately upon receipt - Participate in sales meetings and national trade shows as appropriate and authorized - Conduct Lunch and Learns, workshops, seminars or focus groups at local technical society meetings as appropriate and authorized - Promote the Company as the pathology market leader in quality and innovation Education and Experience Required: - BA/BS in Life Sciences or equivalent required - MBA preferred but not required - 2-5 years Histology/Pathology laboratory experience in clinical , research or industrial setting desirable but not required - Understanding of pathology marketplace with strong technical acumen - 2-5 years knowledge of Company Business Systems, Six Sigma, or Lean Principles required selling experience or consumables - Outstanding problem solving skills. Can manage multiple layers of personnel within customer site 1-3 years Histology laboratory experience in clinical, research or industrial setting desirable but not required - 1-3 years of product management or sales experience in a related discipline preferred but not required Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From LPaveli1 <@t> hurleymc.com Wed Nov 28 07:42:14 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Nov 28 07:42:21 2012 Subject: [Histonet] eosin in the processor In-Reply-To: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> References: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56B479@EXCHANGEMB1.hmc.hurleymc.com> Of our 3- 100% ETOH on our processor, we put about 75mL of LIQUID eosin in our "dirtiest" 100%. Our purchased eosin is made with 95% alcohol, and it works very well, not affecting the processing. We used to use about 1/4 tsp of Eosin Y powder in our "dirtiest" 100%, but as it needs some water to dissolve, it caused trouble in the valves of the processor (oops). We tried using it in our 95%, but it got too washed out by the end of processing. No ill effects on our IHC. Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kim Merriam [kmerriam2003@yahoo.com] Sent: Wednesday, November 28, 2012 7:54 AM To: Histonet Subject: [Histonet] eosin in the processor Hi Everyone, Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues. I can't remember which station we put it in (I think it was the 2nd 100% ethanol). Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure). Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly_colpitts <@t> hotmail.com Wed Nov 28 08:51:02 2012 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Wed Nov 28 08:51:10 2012 Subject: [Histonet] Protocol for preparing Cell Blocks Message-ID: Hi Histo World, We are currently looking at how we process cell blocks and were wondering if anyone had a procedure they would be willing to share with us. Currently, we spin the fluids down in formalin and put the entire button in a biowrap and process that. This leaves our cell blocks very bloody and not easy to embed. I have processed cytology fluids in past jobs where I used cytolyte but I do not have a copy of that protocol. If you would be so kind as to forward me what works for you, that would be great! My email address is kelly.colpitts@nationwidechildrens.org Thanks, Kelly From gayle.callis <@t> bresnan.net Wed Nov 28 09:07:17 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Nov 28 09:07:29 2012 Subject: [Histonet] eosin in the processor In-Reply-To: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> References: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> Message-ID: <000601cdcd7a$10525db0$30f71910$@bresnan.net> The only immunostaining any residual eosin in tissue might affect is if you do immunofluorescence. Eosin does fluoresce. Others can address any effect on IHC. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, November 28, 2012 5:55 AM To: Histonet Subject: [Histonet] eosin in the processor Hi Everyone, Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues. ?I can't remember which station we put it in (I think it was the 2nd 100% ethanol). ?Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure). Thanks, Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaclynch <@t> yahoo.com Wed Nov 28 09:09:05 2012 From: kaclynch <@t> yahoo.com (lynch kenia) Date: Wed Nov 28 09:09:09 2012 Subject: [Histonet] MSI antibodies Message-ID: <1354115345.92172.YahooMailNeo@web112506.mail.gq1.yahoo.com> Need help please......does anyone have a colon block, or would be will ing to cut me a few slides of colon, that has stained positive (which is actually no staining) for the MSI antibodies; MLH1,PMS2, MSH6, MSH2 ? ? Thanks! ? Kenia Lynch, M.T./H.T. Histology/Molecular Supervisor Caldwell Memorial Hospital 321 Mulberry Street SW Lenoir, NC? 28645 From PMonfils <@t> Lifespan.org Wed Nov 28 09:24:59 2012 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Nov 28 09:25:06 2012 Subject: [Histonet] eosin in the processor In-Reply-To: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> References: <1354107295.34329.YahooMailNeo@web121904.mail.ne1.yahoo.com> Message-ID: Eosin won't interfere with binding of antibodies, but eosin is fluorescent. You might want to consider that. From SEsparza <@t> seton.org Wed Nov 28 09:27:05 2012 From: SEsparza <@t> seton.org (Esparza, Sandra E.) Date: Wed Nov 28 09:27:11 2012 Subject: [Histonet] Histologist need in Austin TX.doc Message-ID: <1627CD503B699941A2E8ED164FF3BC9A01440C5AB762@AUSP03VMBX10.apptixhealth.net> Histologist needed in Austin TX We have a full time day position opened for a HT or HTL (ASCP) registered histologist; requires 3 years experience and strong embedding and cutting skills. Experience in muscle enzymes, EM and IHC preferred. If you are interested in learning new techniques and working in a team environment, in a # 1 Trauma Children's hospital with state of the art equipment; in beautiful progressive Austin Texas please call Sandra Esparza BS, HT, QIHC at 512-324-0000 x87062 or fill out an application at seton.org (#040492 Dell Children's). Please note; we are not able to work with recruiters at this time. CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From rjbuesa <@t> yahoo.com Wed Nov 28 09:34:19 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 28 09:34:25 2012 Subject: [Histonet] Waste drum labeling In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC7B5CD4FD@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC7B5CD4FD@JERRY.Gia.com> Message-ID: <1354116859.94606.YahooMailNeo@web163103.mail.bf1.yahoo.com> With different products the toxicity level will vary. Write in the diamond the highest toxic level even if that is not the most abundant. By doing so you cover all inside the drum regarding their toxicity. I do not see the need for the emergency phone but if they told you to so so, do it. Ren? J. From: Amber McKenzie To: Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 28, 2012 1:16 AM Subject: [Histonet] Waste drum labeling For EPA standards, how do you label your waste drums?? I have one 55 lb drum that we only pour ventana waste in.? What numbers do you write in the diamond to cover all the kits that you stain with?? 2 reps from EPA came by my lab yesterday and said i needed an emergency phone list by our phone??? Does anyone have the checklist for EPA that small labs should follow? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cristi Rigazio [cls71877@gmail.com] Sent: Tuesday, November 27, 2012 3:01 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Elizabeth Cameron Subject: Re: [Histonet] Uneven Staining As most other bases have been covered, do you leave space between the slides in the rack?? Once in a blue moon we had stains from the same rack that differed in intensity, after much troubleshooting we started putting space between the slides and we haven't had the problem since.? Just an idea. Cristi Sent from my iPhone On Nov 27, 2012, at 12:24 PM, Rene J Buesa wrote: > This is quite a puzzling situation. > You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of "diluting" in water. > Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. > Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. > Ren? J. > > From: Elizabeth Cameron > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, November 27, 2012 1:35 PM > Subject: [Histonet] Uneven Staining > > About a year ago, I posted about issues with staining mouse kidney sections (see below for original post).? We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale.? This time it seems to be consistent across the slide, so I know it is not a drying issue.? They are serial sections, so they are all cut together and stained together, and they are from the same animal.? I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference.? We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining.? I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath.? They are NBF fixed, and there is a fine line between soaking well for > hydration and oversoaking, resulting in swelling.? Has anyone experienced anything like this?? Any other ideas?? I am at a loss, and I'd really like for this to work! > Thanks, > Liz > > We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys.? We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining.? Half of a section will stain as it should, and the rest of the section is very pale.? It seems to be in a similar area from one section to the next, but not exactly the same area.? It does not look like a deparaffinization issue.? There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine.? The sections are 6 microns. Any ideas on why this might be happening?? Thanks! > > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Wed Nov 28 09:41:39 2012 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Wed Nov 28 09:41:56 2012 Subject: [Histonet] eosin in the processor Message-ID: <08861B9CF6C7774E874635A4818AE37B03791DEA@CHEXCMS01.one.ads.che.org> Hi Kim, try 10cc of the prediluted Eosin (you use in H & E staining) in your last 100% before your Xylene on the processor. By putting it in the last absolute it will not wash out. Your first Xylene will end up a little pink but your eyes will not strain as much trying to fine the tiny tissue fragments. (great for thread-like prostate biopsies too) We had been using it for years & our IHC seems fine. Make sure to keep your solutions for the clean cycles maintenanced on the automatic processors to avoid clogging. The only reason we stopped was the folks we purchased the new processor from told us using it would invalidate the warranty on the new processor.(I really miss it) Cassandra Davis CDavis@che-east.org 302-575-8095 From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kim Merriam [kmerriam2003@yahoo.com] Sent: Wednesday, November 28, 2012 7:54 AM To: Histonet Subject: [Histonet] eosin in the processor Hi Everyone, Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues. I can't remember which station we put it in (I think it was the 2nd 100% ethanol). Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure). Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________ Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From SteveM <@t> mcclainlab.com Wed Nov 28 09:43:46 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Wed Nov 28 09:43:35 2012 Subject: [Histonet] eosin on processor Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF30560BC8@ML1.McClainLabs.local> Eosin on processor can be done with dirty eosin waste saved from the stainer; adding eosin powder sounds like a mess. Especially helpful for orienting small samples with a sidedness, skin GI GU oral, cornea No common down sides for routine HE, PAS, Trichrome, or IHC even with Red chromagens. We leave it off of our research processors, just in case Eosin conflicts with a few obscure extraordinary stains with pale staining, e.g., Acridine Orange or some fluorochromes (fluoroscein), however, given variety of fluorochromes available, use another for your signal and for those others eosin provides a roadmap to navigate the section. Eosin can be made to stain several fungal structures. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From LSebree <@t> uwhealth.org Wed Nov 28 09:54:42 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Nov 28 09:54:50 2012 Subject: [Histonet] Any reference labs offering CD52 (CAMPATH) on human FFPE specimens? Message-ID: <77DD817201982748BC67D7960F2F76AF023FB6@UWHC-MBX12.uwhis.hosp.wisc.edu> Looking for a reference lab offering the above. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From rob <@t> foliobio.com Wed Nov 28 10:00:48 2012 From: rob <@t> foliobio.com (Rob Day) Date: Wed Nov 28 10:00:54 2012 Subject: [Histonet] Any reference labs offering CD52 (CAMPATH) on human FFPE specimens? In-Reply-To: <77DD817201982748BC67D7960F2F76AF023FB6@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF023FB6@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: You might want to try www.phylogenyinc.com. On Nov 28, 2012, at 10:54 AM, Sebree Linda A wrote: > Looking for a reference lab offering the above. > > Thanks, > > Linda A. Sebree > > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > > (608)265-6596 > FAX: (608)262-7174 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Nov 28 10:12:36 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Nov 28 10:12:37 2012 Subject: [Histonet] Quick Question... Message-ID: <00ac01cdcd83$2ee34ab0$8ca9e010$@earthlink.net> Hi Histonetters!! I hope you are having a great day. I have a quick question. Is ASCP certification required in order to work in the state of Michigan? Happy Holidays !! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Valerie.DeMint <@t> phci.org Wed Nov 28 11:24:15 2012 From: Valerie.DeMint <@t> phci.org (DeMint, Valerie S) Date: Wed Nov 28 11:24:25 2012 Subject: [Histonet] FW: Peloris Rapid Tissue Processor Message-ID: <657B95ED46A98B4D9F44EFC67EDCAADD59AF28@RWDEX3.WHS.phci.org> ________________________________ From: DeMint, Valerie S Sent: Tuesday, November 27, 2012 9:29 AM To: 'histonet@lists.utsouthwester.edu' Subject: FW: Peloris Rapid Tissue Processor ________________________________ From: DeMint, Valerie S Sent: Monday, November 26, 2012 3:47 PM To: 'histonet@lists.utsouthwester.edu' Subject: Peloris Rapid Tissue Processor Valerie S. DeMint, HT (ASCP) Waukesha Memorial Hospital Waukesha, WI 53188 I am wondering if anyone has experienced any intermittent, unexplained processing problems with their Peloris rapid tissue processor. I thought our laboratory has had all the correct measures in place to provide us with consistent, good tissue processing result but we continue to have failed runs. Leica technical support cannot locate any problems with our instrument. Here's what we are "using and doing": Careful attention is made so that all specimens are fully fixed prior to starting a processing run. We use ethanol for dehydration. Bottles 3, 4 and 5 are dedicated to graded alcohols at the default concentrations of 70%, 80% and 90%. We are a xylene lab. We are using the recommended xylene protocols and following the tissue size recommendations for each protocol. We use the Activeflow cassettes that are designed to work best with a rapid tissue processor. We do not use anything like biopsy pads that would affect carryover or flow of reagents. Most recently, we had a "bad" run of medium size tissues that were over processed. The only reagent change prior to this run was the 70% alcohol had been changed. Following this bad processing run I checked the alcohol concentration with a hydrometer and it read correctly. There were no error codes on the instrument to indicate a problem. Any ideas or suggestions will be greatly appreciated. This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From rsrichmond <@t> gmail.com Wed Nov 28 12:15:00 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Nov 28 12:15:05 2012 Subject: [Histonet] Re: eosin in the processor Message-ID: Supposedly eosin used to color small specimens, or put in the processor, fluoresces enough in tissue sections to interfere with fluorescent stains. I've seen safranin O (from the microbiology lab) used to mark small surgical specimens like GI biopsies. I don't know whether you can put it in the processor or not. Supposedly safranin O isn't fluorescent, though I haven't verified that. Bob Richmond Samurai Pathoogist Maryville TN From pathlocums <@t> gmail.com Wed Nov 28 12:18:27 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Nov 28 12:18:34 2012 Subject: [Histonet] Deadly Bladex system Message-ID: Does anyone out there use the Bladex system for applying blades to their handles? This unit was forced upon us at one place, required by the OSHA inspectors and specifically told to use Bladex to improve safety in the workplace. I have found that this system not only does not improve safety, but is actually a very unsafe unit. Using Bladex, I have found, the risk of injury is higher than any other means by which we have ever placed a blade on a handle. And it is incredibly expensive to boot. Any thoughts on this unit? Does anyone actually like it, or find it safe? -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From Elizabeth.Cameron <@t> jax.org Wed Nov 28 12:48:53 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Nov 28 12:48:58 2012 Subject: [Histonet] Uneven Staining - Update Message-ID: This morning I decided to pH our DI water, which came out higher than it should. I then did an experiment where I cut a kidney and floated it on three water baths - one a bit basic, one a bit acidic, and one neutral. I cut two sections for each waterbath - one was picked up right away, and the other sat for 10 minutes (we occasionally need to let the kidneys sit to remove wrinkles, although not generally this long). There was no difference between the sections that were picked up immediately and those that sat in the neutral waterbath. The sections from the acidic waterbath did not stain well either way. The sections from the basic water bath stained fine if they were picked up immediately, but did not stain well after sitting for 10 minutes, so I think we?ve figured out the problem. Thanks for all the great suggestions! It's so nice having access to so many knowledgeable histotechs when these strange issues arise! -Liz -----Original Message----- From: Cristi Rigazio [mailto:cls71877@gmail.com] Sent: Tuesday, November 27, 2012 4:01 PM To: Rene J Buesa Cc: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Uneven Staining As most other bases have been covered, do you leave space between the slides in the rack? Once in a blue moon we had stains from the same rack that differed in intensity, after much troubleshooting we started putting space between the slides and we haven't had the problem since. Just an idea. Cristi Sent from my iPhone On Nov 27, 2012, at 12:24 PM, Rene J Buesa wrote: > This is quite a puzzling situation. > You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of "diluting" in water. > Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. > Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. > Ren? J. > > From: Elizabeth Cameron > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, November 27, 2012 1:35 PM > Subject: [Histonet] Uneven Staining > > About a year ago, I posted about issues with staining mouse kidney > sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! > Thanks, > Liz > > We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Helena.Howe <@t> ProMedica.org Wed Nov 28 13:57:26 2012 From: Helena.Howe <@t> ProMedica.org (Howe, Helena) Date: Wed Nov 28 13:57:31 2012 Subject: [Histonet] Validation of a new tissue processor Message-ID: <5288C492C713554297913A98A660A53901549306BC@phsimailcr03.phsi.promedica.org> Does anyone know what the CAP is requiring to validate a new tissue processor? Thank You Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. From brian <@t> prometheushealthcare.com Wed Nov 28 14:28:52 2012 From: brian <@t> prometheushealthcare.com (Brian-Prometheus) Date: Wed Nov 28 14:29:06 2012 Subject: [Histonet] Multiple Histology Mgmt/Supv Openings in NY, NJ, CT Message-ID: <027201cdcda6$fc0c6b70$f4254250$@prometheushealthcare.com> Hope you had a great Thanksgiving! I am currently working on multiple openings in the tri-state area: IHC Supervisor- Suffern NY Lab Operations Manager (over cyto,histo, pre/post technical) Westchester Cty NY Histology Supervisor w Hospital Hartford CT Histology Supervisor reference lab northern NJ If you might know anyone currently looking would greatly appreciate it! Thanks Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From cpyse <@t> x-celllab.com Wed Nov 28 14:44:37 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Nov 28 14:44:46 2012 Subject: [Histonet] chemical disposal Message-ID: <006301cdcda9$2eddd730$8c998590$@com> Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From Joyce.Weems <@t> emoryhealthcare.org Wed Nov 28 14:47:23 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Nov 28 14:47:32 2012 Subject: [Histonet] chemical disposal In-Reply-To: <006301cdcda9$2eddd730$8c998590$@com> References: <006301cdcda9$2eddd730$8c998590$@com> Message-ID: It should be separate. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, November 28, 2012 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] chemical disposal Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mhale <@t> MiracaLS.com Wed Nov 28 14:55:10 2012 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed Nov 28 15:02:06 2012 Subject: [Histonet] California HT Position NEED TO FILL ASAP Message-ID: <0E828EC51C7CC445A51E53F81B64E8C70BFD3F@s-irv-exchmb.PathologyPartners.intranet> Great full time opportunity for a Histotechnician in Yuba City! North Valley GI is looking for certified HT or HTL to join their laboratory . HT candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT/HTL ASCP Certified , Bachelor's degree or Associates is required .Supervisory experience is preferred. Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed These positions offer a competitive rate of $35.00 an hour and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From chapcl <@t> yahoo.com Wed Nov 28 15:14:26 2012 From: chapcl <@t> yahoo.com (Will Chappell) Date: Wed Nov 28 15:14:36 2012 Subject: [Histonet] chemical disposal In-Reply-To: <006301cdcda9$2eddd730$8c998590$@com> References: <006301cdcda9$2eddd730$8c998590$@com> Message-ID: Chemical hazardous waste. Sent from my iPhone On Nov 28, 2012, at 12:44 PM, "Cynthia Pyse" wrote: > Quick question for Histoland. I am having a debate about DAB disposal. Our > general manager ( non lab background) insists that the liquid DAB can go > into a biological hazardous waste. I disagree, it is a chemical and needs to > be disposed in the chemical hazardous waste. What is everyone else doing to > dispose of DAB. We are located in NY, I do have those regs. Thanks in > advance for any and all help. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 20 Northpointe Parkway Suite 100 > > Amherst, NY 14228 > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Nov 28 15:46:33 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 28 15:46:38 2012 Subject: [Histonet] Validation of a new tissue processor In-Reply-To: <5288C492C713554297913A98A660A53901549306BC@phsimailcr03.phsi.promedica.org> References: <5288C492C713554297913A98A660A53901549306BC@phsimailcr03.phsi.promedica.org> Message-ID: <1354139193.94292.YahooMailNeo@web163103.mail.bf1.yahoo.com> Using at least 25 different types of tissues, prepare parallel almost identical pairs of?slices for each tissue and process them simultaneously in your new tissue processor and in the?old one. Prepare H&E sections and give them to the pathologists concealing the used tissue processor. The pathologists should decide if there are differences between the pairs of sections. The pathologist's opinion?should be registered in the QC file and that will be your validation. Ren? J. From: "Howe, Helena" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, November 28, 2012 2:57 PM Subject: [Histonet] Validation of a new tissue processor Does anyone know what the CAP is requiring to validate a new tissue processor? Thank You Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Nov 28 15:48:27 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 28 15:48:34 2012 Subject: [Histonet] chemical disposal In-Reply-To: <006301cdcda9$2eddd730$8c998590$@com> References: <006301cdcda9$2eddd730$8c998590$@com> Message-ID: <1354139307.678.YahooMailNeo@web163103.mail.bf1.yahoo.com> You are right and your "general manager" is wrong (as they usually?are when dealing with histology issues!). Ren? J. From: Cynthia Pyse To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 28, 2012 3:44 PM Subject: [Histonet] chemical disposal Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Wed Nov 28 15:50:30 2012 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Nov 28 15:50:35 2012 Subject: [Histonet] Validation of a new tissue processor In-Reply-To: <1354139193.94292.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <5288C492C713554297913A98A660A53901549306BC@phsimailcr03.phsi.promedica.org> <1354139193.94292.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: You will also need to validate all of the IHC stains and special stains that you do on these tissues. You can take samples from each tissue type and run them in parallel on both machines. Then test all of your special stains and antibodies that you have in your inventory. :) Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, November 28, 2012 4:47 PM To: Howe, Helena; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Validation of a new tissue processor Using at least 25 different types of tissues, prepare parallel almost identical pairs of?slices for each tissue and process them simultaneously in your new tissue processor and in the?old one. Prepare H&E sections and give them to the pathologists concealing the used tissue processor. The pathologists should decide if there are differences between the pairs of sections. The pathologist's opinion?should be registered in the QC file and that will be your validation. Ren? J. From: "Howe, Helena" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, November 28, 2012 2:57 PM Subject: [Histonet] Validation of a new tissue processor Does anyone know what the CAP is requiring to validate a new tissue processor? Thank You Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dreynold <@t> mdanderson.org Wed Nov 28 16:07:55 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Wed Nov 28 16:08:23 2012 Subject: [Histonet] microarray on OCT blocks Message-ID: <785BBF0C5F49CE41BA74460A43A08F02340A814BC8@DCPWVMBXC0VS3.mdanderson.edu> I have an investigator wanting to make microarrays from some frozen OCT blocks. I seem to remember reading a way to do this. Can someone please point me in the right direction? Thanks Donna Reynolds, HT (ASCP) Chief IHC Core Lab Dept. Cancer Biology, Univ. Tex. M.D. Anderson Cancer Center Houston, Texas 713-792-8106 From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 28 16:10:08 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 28 16:10:28 2012 Subject: [Histonet] Validation of a new tissue processor In-Reply-To: References: <5288C492C713554297913A98A660A53901549306BC@phsimailcr03.phsi.promedica.org> <1354139193.94292.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF02DE16@ex07.net.ucsf.edu> I don't think it is necessary to test every single stain or antibody you do to validate a new tissue processor. Most of the time the only thing changing is the machine, not the chemicals you use. The point of validation is to prove that the system gives you the same results as the old instrument. Using a representative sample of types of stains and antibodies will give you confidence that it works fine and save a huge amount of money and time. Think about it from this perspective: We get paraffin slides and blocks from consult cases from all over the place, processed on many different machines with infinitely variable time and chemical protocols. Yet virtually all the cases work fine in our immuno staining system. It is rare to find cases that do not. That alone tells you that massive validation is not really necessary. The point of a validation procedure is to plan one that you feel will give you confidence that the new instrument gives you the same results as the old one. If you do insist on testing "all" your antibodies (we have over 200 here) then tissue arrays or multiple tissue blocks will save a lot of time and money. Of course, if you go to a new processor that uses totally different types of chemicals and methods then validation of every antibody may be necessary. Even then, however, representative markers of various types may suffice. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Wednesday, November 28, 2012 1:51 PM To: 'Rene J Buesa'; Howe, Helena; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Validation of a new tissue processor You will also need to validate all of the IHC stains and special stains that you do on these tissues. You can take samples from each tissue type and run them in parallel on both machines. Then test all of your special stains and antibodies that you have in your inventory. :) Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, November 28, 2012 4:47 PM To: Howe, Helena; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Validation of a new tissue processor Using at least 25 different types of tissues, prepare parallel almost identical pairs of?slices for each tissue and process them simultaneously in your new tissue processor and in the?old one. Prepare H&E sections and give them to the pathologists concealing the used tissue processor. The pathologists should decide if there are differences between the pairs of sections. The pathologist's opinion?should be registered in the QC file and that will be your validation. Ren? J. From: "Howe, Helena" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, November 28, 2012 2:57 PM Subject: [Histonet] Validation of a new tissue processor Does anyone know what the CAP is requiring to validate a new tissue processor? Thank You Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 <@t> gmail.com Wed Nov 28 16:45:38 2012 From: steve8438 <@t> gmail.com (Steven Mello) Date: Wed Nov 28 16:45:50 2012 Subject: [Histonet] chemical disposal In-Reply-To: <1354139307.678.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <006301cdcda9$2eddd730$8c998590$@com> <1354139307.678.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: Separate and chemical hazardous waste! Steven Mello,HT(ASCP) Sent from my iPad On Nov 28, 2012, at 4:48 PM, Rene J Buesa wrote: > You are right and your "general manager" is wrong (as they usually are when dealing with histology issues!). > Ren? J. > > From: Cynthia Pyse > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, November 28, 2012 3:44 PM > Subject: [Histonet] chemical disposal > > Quick question for Histoland. I am having a debate about DAB disposal. Our > general manager ( non lab background) insists that the liquid DAB can go > into a biological hazardous waste. I disagree, it is a chemical and needs to > be disposed in the chemical hazardous waste. What is everyone else doing to > dispose of DAB. We are located in NY, I do have those regs. Thanks in > advance for any and all help. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 20 Northpointe Parkway Suite 100 > > Amherst, NY 14228 > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Wed Nov 28 18:07:52 2012 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Nov 28 18:08:00 2012 Subject: [Histonet] Job Opening Message-ID: <6ED9D4252F278841A0593D3D788AF24C17D300B4@mailsvr.MARSHMED.local> We are a small pathology in the Sacramento area (Placerville) looking for a certified histotech. This is a full time position that offers competitive compensation and full benefits. You can forward your information to Gary Martin at gmartin@marshallmedical.org. From tkngflght <@t> yahoo.com Wed Nov 28 20:07:55 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Nov 28 20:08:00 2012 Subject: [Histonet] short temp but home for Christmas Message-ID: <1354154875.40158.YahooMailClassic@web39404.mail.mud.yahoo.com> Hi All- ? Looking for a happy person and a good cutter, preferrably the same person!? ? A short day shift temp, M-F from ASAP through the Friday before Christmas.? Fill up the bank account and be home for the holidays.? Experience with small biopsies required.? Full travel including per diems, hotel, flights and car rental (or reimbursement for using your own vehicle).? ? For more info, call my cell and leave a message--I'll call you back across the afternoon.? It's one of the nicest little labs in the South! ? 281.883.7704 Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From lpwenk <@t> sbcglobal.net Thu Nov 29 03:53:57 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Nov 29 03:54:00 2012 Subject: [Histonet] chemical disposal In-Reply-To: <006301cdcda9$2eddd730$8c998590$@com> References: <006301cdcda9$2eddd730$8c998590$@com> Message-ID: Where most people get confused is the"Biological Hazard". They think - this chemical would hurt a human being, it would damage someone's skin if splashed on it, it would injure someone's lungs if inhaled, it could cause cancer with long exposure, etc. Since it's hurting a person, it must be a biological hazard. Nope. "Biological hazard" means that the "solution" contains a biologically active microorganism (bacteria, fungus, virus, parasite) or a prion - all of which could infect the person using the "solution" if not handled correctly. (Fresh tissue would be a possible biological hazard, since we don't know what, if anything, that tissue is infected with. Same with a frozen section. If tissue is properly fixed and processed, that would kill the microorganism, so the tissue is no longer a biological hazard. Prions need special treatment with formic acid.) If the material is a chemical (solvent, dye, bleach, soap, toner cartridges that leak, etc.), then it is a Chemical Hazard. This would include the DAB. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on Beaumont Hospital. -----Original Message----- From: Cynthia Pyse Sent: Wednesday, November 28, 2012 3:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] chemical disposal Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Nov 29 08:07:40 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Nov 29 08:07:44 2012 Subject: [Histonet] RE: My earlier post about ASCP requirements in MI Message-ID: <007901cdce3a$e56b9370$b042ba50$@earthlink.net> Hi Histonetters!! Thank you to all my histobuddies that responded about the certification in Michigan question. Of course I am going to encourage them to hire a certified tech it was a question the client asked that I needed to get answered and I appreciate you taking the time. Happy Holidays!! Happy Holidays !! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa <@t> yahoo.com Thu Nov 29 09:13:23 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 29 09:13:34 2012 Subject: [Histonet] Validation of a new tissue processor In-Reply-To: <761E2B5697F795489C8710BCC72141FF02DE16@ex07.net.ucsf.edu> References: <5288C492C713554297913A98A660A53901549306BC@phsimailcr03.phsi.promedica.org> <1354139193.94292.YahooMailNeo@web163103.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02DE16@ex07.net.ucsf.edu> Message-ID: <1354202003.23864.YahooMailNeo@web163103.mail.bf1.yahoo.com> Absolutely agree with Tim's comments. I would even add that if the new tissue processor uses the "standard" heat/agitation/vacuum/pressue sequences as the old one, the validation objective is?mainly directed to have some documentation that the facility's attorneys can have in case "something goes wrong" and the plaintiff's attorney asks: "Was the instrument validated?" avoiding that a judge, also ignorant of our methods, rules against?our lab. The validation is really necessary if the processing technology goes from the "conventional" to the microwave technology where the times are so different and the development of new?quality protocols are of paramount importance.? The one who has to decide about the validation test is the pathologist and his acceptance has to go to the QC file, and that's it! Ren? J.? From: "Morken, Timothy" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, November 28, 2012 5:10 PM Subject: RE: [Histonet] Validation of a new tissue processor I don't think it is necessary to test every single stain or antibody you do to validate a new tissue processor. Most of the time the only thing changing is the machine, not the chemicals you use. The point of validation is to prove that the system gives you the same results as the old instrument. Using a representative sample of types of stains and antibodies will give you confidence that it works fine and save a huge amount of money and time. Think about it from this perspective: We get paraffin slides and blocks from consult cases from all over the place, processed on many different machines with infinitely variable time and chemical protocols. Yet virtually all the cases work fine in our immuno staining system. It is rare to find cases that do not. That alone tells you that massive validation is not really necessary. The point of a validation procedure is to plan one that you feel will give you confidence that the new instrument gives you the same results as the old one. If you do insist on testing "all" your antibodies (we have over 200 here) then tissue arrays or multiple tissue blocks will save a lot of time and money. Of course, if you go to a new processor that uses totally different? types of chemicals and methods then validation of every antibody may be necessary. Even then, however, representative markers of various types may suffice. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Wednesday, November 28, 2012 1:51 PM To: 'Rene J Buesa'; Howe, Helena; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Validation of a new tissue processor You will also need to validate all of the IHC stains and special stains that you do on these tissues. You can take samples from each tissue type and run them in parallel on both machines. Then test all of your special stains and antibodies that you have in your inventory. :) Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, November 28, 2012 4:47 PM To: Howe, Helena; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Validation of a new tissue processor Using at least 25 different types of tissues, prepare parallel almost identical pairs of?slices for each tissue and process them simultaneously in your new tissue processor and in the?old one. Prepare H&E sections and give them to the pathologists concealing the used tissue processor. The pathologists should decide if there are differences between the pairs of sections. The pathologist's opinion?should be registered in the QC file and that will be your validation. Ren? J. From: "Howe, Helena" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, November 28, 2012 2:57 PM Subject: [Histonet] Validation of a new tissue processor Does anyone know what the CAP is requiring to validate a new tissue processor? Thank You Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rags_jeg <@t> yahoo.co.in Thu Nov 29 10:25:33 2012 From: rags_jeg <@t> yahoo.co.in (vaigunda ragavendran) Date: Thu Nov 29 10:25:45 2012 Subject: [Histonet] Hii everybody Message-ID: <1354206333.93500.YahooMailNeo@web192405.mail.sg3.yahoo.com> Hii Histonetters, Happy holidays. I am on the lookout for a marker to be checked using immunohistochemistry that would get expressed at the DRG in response to streptozotocin-induced diabetes. I am basically looking for a neuronal marker that would give an idea of the degree of microvascular dysfunction induced by diabetes. Sounds very complicated though. Any suggestions are most welcome. Thanks and bye. Rags, Postdoctoral Research Assistant, Coderre Lab, Department of Anesthesia, Room No: 1201, McIntyre Medical Building, 3655, Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada. Phone (Lab): +1 514-398-2903 (Facsimile): +1 514-398-8241 From zakkyums <@t> gmail.com Thu Nov 29 10:46:03 2012 From: zakkyums <@t> gmail.com (zakky cholisoh) Date: Thu Nov 29 10:46:06 2012 Subject: [Histonet] Lung histology Message-ID: Hello lovely people! Is there anyone working with mouse lung (or any lung)? I work with respiratory virus in mice and now about to begin the fun part of the work, histopathology :) I was just wondering what is the best section of the lung, is it coronal section or tranversal (horizontal) section? Any advice? Thanks so much in advance Zakky PhD student Respiratory Pharmacology Welsh School of Pharmacy From delsuec <@t> gmail.com Thu Nov 29 10:59:43 2012 From: delsuec <@t> gmail.com (Deloris Carter) Date: Thu Nov 29 10:59:50 2012 Subject: [Histonet] Radiatiation Safety Message-ID: Hi all, I'm trying to complete a protocol for radioactive specimen handling. I need to know if anyone has suggestions for this. We do not use a geiger counter for specimens. The radiation safety person in nuclear med says their policy is that technicium 99 has such a short half life, and that there is no need for special handling of sentinel node specimens. I just need some input. I'm not really getting anywhere with nuclear med on any other information such as prostate seeds, the actual breast biopsy that may be sent after a sentinel node procedure, etc. Our CAP inspection looms, and I need to finalize this protocol ASAP. Thanks for any help. Deloris Carter HT(ASCP) delsuec@gmail.com From peter.craven <@t> nhs.net Thu Nov 29 11:08:42 2012 From: peter.craven <@t> nhs.net (Craven Peter (NHS HIGHLAND)) Date: Thu Nov 29 11:12:02 2012 Subject: [Histonet] Radiatiation Safety In-Reply-To: <20121129170034.CE040478779@nhs-pd1e-esg002.ad1.nhs.net> References: <20121129170034.CE040478779@nhs-pd1e-esg002.ad1.nhs.net> Message-ID: <20121129171155.3C0A2448F61@nhs-pd1e-esg102.ad1.nhs.net> Deloris When we set this up we got our Radiation Protection Services to write a safe handling policy for the tissues (sentinel nodes) and added this into our Standard Operating Procedures. Might as well get the advice from the experts and trust it rather than making your own policy. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital Old Perth Road Inverness IV2 3UJ Tel 01463 704269 email peter.craven@nhs.net ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter [delsuec@gmail.com] Sent: 29 November 2012 04:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Radiatiation Safety Hi all, I'm trying to complete a protocol for radioactive specimen handling. I need to know if anyone has suggestions for this. We do not use a geiger counter for specimens. The radiation safety person in nuclear med says their policy is that technicium 99 has such a short half life, and that there is no need for special handling of sentinel node specimens. I just need some input. I'm not really getting anywhere with nuclear med on any other information such as prostate seeds, the actual breast biopsy that may be sent after a sentinel node procedure, etc. Our CAP inspection looms, and I need to finalize this protocol ASAP. Thanks for any help. Deloris Carter HT(ASCP) delsuec@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From madeleinehuey <@t> gmail.com Thu Nov 29 13:07:13 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Nov 29 13:07:17 2012 Subject: [Histonet] Re: Histonet Digest, Vol 108, Issue 37 In-Reply-To: <50b7a2f6.466fb60a.65b8.ffffe0f4SMTPIN_ADDED_MISSING@mx.google.com> References: <50b7a2f6.466fb60a.65b8.ffffe0f4SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Message: 2 Date: Thu, 29 Nov 2012 16:46:03 +0000 From: zakky cholisoh Subject: [Histonet] Lung histology To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello lovely people! Is there anyone working with mouse lung (or any lung)? I work with respiratory virus in mice and now about to begin the fun part of the work, histopathology :) I was just wondering what is the best section of the lung, is it coronal section or tranversal (horizontal) section? Any advice? Thanks so much in advance Zakky PhD student Respiratory Pharmacology Welsh School of Pharmacy Hello Zakky, It all depends! One of my previous project I am screening tumors from the mouse lungs, therefore I always do them on Tranversal (seperate all 5 lobes first, then embed horizontal). The thinner the block (horizontal) the lesser sections I need to cut. Hope this answer your questions. Madeleine Huey BS, HTL & QIHC (ASCP) Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org From CDavis <@t> che-east.org Thu Nov 29 13:08:05 2012 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Nov 29 13:08:21 2012 Subject: [Histonet] cellblock help Message-ID: <08861B9CF6C7774E874635A4818AE37B03791DEC@CHEXCMS01.one.ads.che.org> Good afternoon Histonet folks, I am hoping somebody might be able to help me with a protocol using Thrombin to get a cellblock from a specimen with scant material. This process was brought to my attention a few years ago but I never saw an actual protocol. Thanks for any help & have an awesome day. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From madeleinehuey <@t> gmail.com Thu Nov 29 13:26:43 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Nov 29 13:26:48 2012 Subject: [Histonet] Re: Histonet Digest, Vol 108, Issue 34 In-Reply-To: <50b6294e.6534b60a.3c30.ffff83c6SMTPIN_ADDED_MISSING@mx.google.com> References: <50b6294e.6534b60a.3c30.ffff83c6SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: On Wed, Nov 28, 2012 at 7:10 AM, wrote: > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kim Merriam [kmerriam2003@yahoo.com] > Sent: Wednesday, November 28, 2012 7:54 AM > To: Histonet > Subject: [Histonet] eosin in the processor > > Hi Everyone, > > Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues. I can't remember which station we put it in (I think it was the 2nd 100% ethanol). Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure). > > Thanks, > Kim > > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA Hello Kim, I always put the Eosin in the last two 100% alcohol. The whole concepts is to have your tissues pick up the Eosin and not wash out during the processing. Therefore if you put them in the beginning (ie.70%, 85%.... ) and not the 100% alcohol, they will eventually be wash out. Madeleine Huey BS, HTL & QIHC (ASCP) Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org From jamie.erickson <@t> abbott.com Thu Nov 29 13:28:00 2012 From: jamie.erickson <@t> abbott.com (Erickson, Jamie E) Date: Thu Nov 29 13:28:11 2012 Subject: [Histonet] Re: Lung histology Message-ID: <8B946A68A8F3534A99CC493DEFB49B10A4198B@WM10002P.oneabbott.com> HI Zakky, I've worked on a lot on mouse lung and the process of embedding can depend on what you are looking for or your pathologist opinion. In my OVA models I was looking at inflammation around the main stem bronchi and looking at goblet cell hyperplasia using PAS stain. Because we used an intranasal route of administration we wanted to look to see if there was a difference in PAS at the main stem bronchi compared to distal secondary and tertiary bronchi so we chose to embed sagittal. I isolated the left lobe that is the lobe with no accessory lobes then trimmed in to the main stem but not to close, this was done to make a flat surface for embedding. Then you can cut into the lung until you get a nice full or almost full length of the main stem bronchi. I did PAS on these and image analysis to quantitate amount of PAS production. Perfusion of the lungs was a must for this view. There are lots of papers that do this by cross sections as well and stain with collagen deposition stains like sirus red, so you'll have to see what fits your study. Whatever you choose be consistent with your location of sectioning, so you can compare samples across groups that are taken from the same anatomical location. Hope that helps Good luck, Jamie Erickson (ASCP) HTL Pharmacology Dept. Abbott laboratories From rsrichmond <@t> gmail.com Thu Nov 29 21:12:55 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Nov 29 21:13:01 2012 Subject: [Histonet] Re: Radiation safety Message-ID: Deloris Carter (where?) asks: >>I'm trying to complete a protocol for radioactive specimen handling. I need to know if anyone has suggestions for this. We do not use a geiger counter for specimens. The radiation safety person in nuclear med says their policy is that technicium 99 has such a short half life, and that there is no need for special handling of sentinel node specimens. I just need some input. I'm not really getting anywhere with nuclear med on any other information such as prostate seeds, the actual breast biopsy that may be sent after a sentinel node procedure, etc. Our CAP inspection looms, and I need to finalize this protocol ASAP. Thanks for any help.<< Technetium 99m has a half-life of six hours, decaying (emitting a gamma particle) into technetium 99 which has a half-life of over 100,000 years. The amount of radioactive material in a sentinel node or a lumpectomy specimen is so small that no special precautions are needed in handling and processing it. There are a number of good articles about this available - I can probably find some references - but this matter was put to rest about 15 years ago. The radioisotopes in prostate "seeds" are more hazardous. There are three such isotopes, if all of them are still in use. The longest-lived has a half-life of 73 days, so it takes about two years for it to decay to a reasonably safe level. I've received "seeds" in prostatectomy (TURP) specimens, with no information as to what they were or how old they were. Although they are probably not very hazardous, I consider this to be serious negligence. Here's what they look like, from a specimen I had several years ago (the "seeds" were four years old, I finally found out). http://www.flickr.com/photos/bobrichmond/5833004125/in/set-72157618450128961 I would suggest not processing the specimen (or opening the container) until adequate information is obtained. Expect to be treated with great condescension when you inquire. I don't know of any references on the subject. I don't know how CAP addresses the problem. Bob Richmond Samurai Pathologist Maryville TN From l.kammili <@t> yahoo.com Thu Nov 29 22:36:39 2012 From: l.kammili <@t> yahoo.com (Lakshmi Kammili) Date: Thu Nov 29 22:36:45 2012 Subject: [Histonet] CRYOSTAT PROBLEM Message-ID: <1354250199.66943.YahooMailNeo@web113714.mail.gq1.yahoo.com> Hi all, We have a old cryostat (Microm h505 model). It was making some sudden vibrating?noises for a little while and then?smooths?it self. There??are no temperature alterations every thing was fine but?suddenly it stopped working, no display more looks like dead :(? I tried calling the service company which serviced before but I guess the company is no more in service (as it was a very old machine). I am very new to this?maintenance?issues , I would be glad if any one of you can help me in suggesting a company or person whom I can talk about the issue or some one who can come and look at the ?instrument and suggest the best advice?. I assume probably some electric motor is dead inside.? Any advice will be really helpful. Thank you, Lakshmi, Sr. research assistant, Pathology core Lab, George Washington University, Washington, DC. From ratliffjack <@t> hotmail.com Fri Nov 30 00:45:01 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Nov 30 00:45:20 2012 Subject: [Histonet] Microtome knives In-Reply-To: <781F33CD0401495DB2AAE3418FBA9F18@DESKTOP3> References: , <1352627080.42134.YahooMailNeo@web171201.mail.ir2.yahoo.com>, <781F33CD0401495DB2AAE3418FBA9F18@DESKTOP3> Message-ID: I might also add that Delaware Diamond Knives (DDK) also sells and sharpens microtome knives! Jack Jack L RatliffRatliff Histology Consultants, LLC317-281-1975 > From: pruegg@ihctech.net > To: max_histo_00@yahoo.it; jkrupp@deltacollege.edu; Histonet@lists.utsouthwestern.edu > Date: Sun, 18 Nov 2012 07:50:11 -0700 > Subject: RE: [Histonet] Microtome knives > CC: > > There are knife sharpening services Sturkey and Dorn and Hart are two that > come to mind. You can also find refurbished disposable blade holders and > buy disposable blades, the blade holder you get will determine if you use > low profile or high profile blades. > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting, LLC > 40864 Arkansas Ave > Bennett, CO 80102 > Phone: 303-644-4538 > Fax: 720-859-4110 > pruegg@ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Massimo > Sent: Sunday, November 11, 2012 2:45 AM > To: Jon Krupp; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome knives > > I prefer to sharpen my microtome knives by myself by hand. > I have a vintage Cambridge Rocking Microtome and despite its age it works > very well. > Sharpening is a time consuming for the first time, it's depends on the > conditions of the blade edge. > Once you have a nice cutting profile its maintenance it's quite easy and it > takes a few minutes by > stroking the knife on a flat glass with oil and a bit of aluminium oxide > powder (3 -1 micron grits). > For me sharpening and honing of a microtome knife has became a secondary > "hobby". > A solid knife has the advantage, compared to a disposable blade, to be > liable to less vibrations. > > Kind Regards, > Massimo Tosi > > > "A humble Chemical > Engineer who loves Histology" > > > > > > ________________________________ > Da: Jon Krupp > A: Histonet@lists.utsouthwestern.edu > Inviato: Venerd? 9 Novembre 2012 19:49 > Oggetto: [Histonet] Microtome knives > > Greetings > > I need some advice regarding microtome knives. I am not histo tech, I did > all my sectioning in a plant research lab, but now I find myself needing to > learn more about histo type methods. > > We have microtomes, AO 820's, and we have a bunch of donated knives. I need > advice about whether it would be better to find a knife sharpener and use > the microtome knives we have, or check into getting a disposable knife > holder. > > When I was sectioning, we just used a simple razor blade holder. Now I see > references to high profile and low profile blades and holders, and I don't > know the difference. > > Anyone willing to help me out? > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From W.E.J.Hoekert <@t> olvg.nl Fri Nov 30 03:54:59 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Nov 30 03:55:14 2012 Subject: [Histonet] MSI antibodies References: <1354115345.92172.YahooMailNeo@web112506.mail.gq1.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F842AF@PAIT42.olvg.nl> Hi Kenia I guess you will need at least two blocks because they are never 'positive' all four of them in the same tumour. You might search your archive for young people with colon cancer. There is a good chance that you will find positive material. Willem Hoekert OLVG The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens lynch kenia Verzonden: wo 28-11-2012 16:09 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] MSI antibodies Need help please......does anyone have a colon block, or would be will ing to cut me a few slides of colon, that has stained positive (which is actually no staining) for the MSI antibodies; MLH1,PMS2, MSH6, MSH2 ? Thanks! Kenia Lynch, M.T./H.T. Histology/Molecular Supervisor Caldwell Memorial Hospital 321 Mulberry Street SW Lenoir, NC 28645 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From melissa <@t> alliedsearchpartners.com Fri Nov 30 09:31:40 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Fri Nov 30 09:31:55 2012 Subject: [Histonet] Cytotechnologist Job (Supervisory/Senior) Message-ID: Hello All, Happy Friday! We have a Cytotechnologist (Supervisory/Senior) position available for full time/permanent hire in Charlotte, NC area. Please email me if you are interested. Thank you, To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From Chris <@t> bakopathology.com Fri Nov 30 09:48:09 2012 From: Chris <@t> bakopathology.com (Chris Winans) Date: Fri Nov 30 09:48:13 2012 Subject: [Histonet] Flashcards Message-ID: Hi, I am looking to purchase a set of the newer Flashcards offered by the ASCP for the HIstotechnician exam. Thanks! ENFD Supervisor Bako Pathology Services Chris@bakopathology.com From JMacDonald <@t> mtsac.edu Fri Nov 30 11:52:38 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Nov 30 11:52:43 2012 Subject: [Histonet] Flashcards In-Reply-To: References: Message-ID: You can purchase them from the NSH or from the ASCP. < wrote: To: "histonet@l From: Sent by: histonet-bounces@lists. Date: 11/30/2012 07:49AM Subject: [Histonet] Flash Hi, I am looking to purchase a set of the newer the ASCP for the HIstotechnician exam. Thanks! ENFD Supervisor Bako Pathology Services Chris@bakopathology.com<< href="mailto:Chris@bakopathology.com">mailto:Chris@bakopathology.com > ___________________ _______________________ 5F_ Histonet mailing list Histonet@lists.utsouthwestern.edu References 1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Tim.Coulter <@t> belairinc.com Fri Nov 30 12:45:30 2012 From: Tim.Coulter <@t> belairinc.com (Tim Coulter) Date: Fri Nov 30 12:44:29 2012 Subject: [Histonet] RE: CRYOSTAT PROBLEM Message-ID: <0183E6EF47BD9B499D49AE65EE9D0189042EE1DC@BIC05.bic.local> Hello Lakshmi, We can help with your cryostat problem. Our company, Belair Instrument Co, has been repairing histology equipment for over 30 years and we have a service office in College Park, MD - not far away from you. We'd be glad to assist, just give us a call at 973 912 8900 and ask for the service desk. Best Regards, Tim Coulter Operations Manager | Belair Corporate Group tim.coulter@belairinc.com | p 973.912.8900 ext. 174 | f 973.232.0077 Avantik Biogroup | Belair Instrument Co. | Sierra Biosystems 36 Commerce Street | P.O. Box 619 | Springfield, NJ 07081-0619 Legal Disclaimer: This email is confidential and may contain legally privileged information. If you are not the intended recipient, you must not disclose or use the information contained in it. If you have received this email in error, please notify us immediately by return email and delete this document. Date: Thu, 29 Nov 2012 20:36:39 -0800 (PST) From: Lakshmi Kammili Subject: [Histonet] CRYOSTAT PROBLEM To: histonet Message-ID: <1354250199.66943.YahooMailNeo@web113714.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, We have a old cryostat (Microm h505 model). It was making some sudden vibrating?noises for a little while and then?smooths?it self. There??are no temperature alterations every thing was fine but?suddenly it stopped working, no display more looks like dead :(? I tried calling the service company which serviced before but I guess the company is no more in service (as it was a very old machine). I am very new to this?maintenance?issues , I would be glad if any one of you can help me in suggesting a company or person whom I can talk about the issue or some one who can come and look at the ?instrument and suggest the best advice?. I assume probably some electric motor is dead inside.? Any advice will be really helpful. Thank you, Lakshmi, Sr. research assistant, Pathology core Lab, George Washington University, Washington, DC. From cpyse <@t> x-celllab.com Fri Nov 30 15:21:30 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Nov 30 15:21:39 2012 Subject: [Histonet] thanks Message-ID: <006101cdcf40$aa8a7670$ff9f6350$@com> I want to thank everyone who responded to my chemical disposal question. I now have adequate ammunition to combat my general manager's lack of knowledge. He seems to think I don't know what I am talking about. Thanks again for the backup it is appreciated. Have a great weekend. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From pruegg <@t> ihctech.net Fri Nov 30 21:53:33 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 30 21:53:37 2012 Subject: [Histonet] 1 gal paraffin dispenser for training in Cozumel Mexico Message-ID: <277B04AB8E5B483FBC125D3231911D7F@DESKTOP3> I am posting this for a colleague David Davis who is doing some training of techs in Cozumel and is really in need of a 1 gal paraffin dispenser for that. I have a 5 gal dispenser to donate but he thinks that is too big. If anyone has a 1 gal paraffin dispenser they would be willing to donate to this cause we could probably provide you with a fedex acct no for shipping it to use. Thank you for considering this request, Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net