From rjbuesa <@t> yahoo.com Tue May 1 06:53:28 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 1 06:53:31 2012 Subject: [Histonet] Productivity Reports In-Reply-To: Message-ID: <1335873208.39081.YahooMailClassic@web162105.mail.bf1.yahoo.com> Productivity figures are fundamental to justify the staff you have or the one you will need if a work growth is expected or planned by your director. There are many ways of figuring out productivity and not all tasks can be measured in the same way. For instance: 1- FS productivity ought to be measured in the time (minutes) elapsing between the moment the specimen reached the FS area and the moment the histotech (HT)?delivers to the pathologist (PT) the slides ready to read. By the way, this is the only task that CAP has developed a productivity standard and it is 20 minutes and the PT has to justify if any FS takes more than the required?20 minutes. As to the HT, the average productivity if 15 minutes. 2- For the lab supervisor, two productivity indices are casted blocks per hour, while embedding or blocks per hour while sectioning, and the list can be expanded to other tasks where the work unit is the block. 3- all in all, the Canadian model of measuring productivity, that I have included in my calculations, refers to the number of blocks (or cases in other occasions) divided by the?total time it elapses between the moment the case is received at the lab. until the diagnosis is sent to the physician. That would be the Total Gross Work flow productivity. ? There are many ways but what is absolutely certain is that if you want to have a grasp of your lab and staff and control their activities, as well as analyze the whole operation to improve the work flow, you need to determine productivity. There are always those that, with reason, argue that excessive productivity can "damage" quality. The argument seems to be correct in general but in no way it can deter trying to be as productive as possible and maintain quality. There should be no compromises either way. An "ideal" quality cannot affect productivity, and viceversa. Under separate cover I am sending 2 works I published on this issue. You should show them to your boss and let him/her select which productivity indices s/he prefers. Ren? J. --- On Mon, 4/30/12, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Productivity Reports To: "histonet@lists.utsouthwestern.edu" Date: Monday, April 30, 2012, 5:24 PM Hello to all in histoland.? Does any one have to do productivity reports for their boss.? If so, what are you using for your unit of measure to say if your techs are being productive.? My boss is really into the numbers, graphs and charts.? I have a my techs fill out a workload recording form.? I really don't know where to begin trying to pull data from it for a report.? If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Tue May 1 08:42:25 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Tue May 1 08:42:35 2012 Subject: [Histonet] Part Time MOHS Tech Needed ASAP Message-ID: Allied Search Partners is working with a small laboratory who is in need of a Part Time MOHS Histotech. This position is an immediate need. We are looking for someone who has experience in doing MOHS Histology. To apply: Please send resume to Melissa@alliedsearchpartners.com or fax to 888 388 7572. Please note: No other information is disclosed about the organization until we receive your resume for review. All qualified applicants will be contacted with further information prior to an interview being scheduled for the position. Thank you! Location(s): Mt. Pleasant, WI 3 or 4 days per week and 1?2 a Day in Franklin, WI Schedule: Monday-Friday Part Time Hours. This position has the potential to become a full time position. Travel: Majority of the work is in Mt. Pleasant, WI area and 1?2 a Day in Franklin, WI area. Requirements: Ability to travel between two locations on one day per week MOHS Histology Experience Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From FUNKM <@t> mercyhealth.com Tue May 1 10:44:06 2012 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue May 1 10:44:15 2012 Subject: [Histonet] Productivity Reports In-Reply-To: References: Message-ID: <4F9FBE76020000AC00010179@nodcdmg2.no.trinity-health.org> Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "Scott, Allison D" 04/30/2012 4:24 PM >>> Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue May 1 11:01:24 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue May 1 11:01:24 2012 Subject: [Histonet] Productivity Reports In-Reply-To: <4F9FBE76020000AC00010179@nodcdmg2.no.trinity-health.org> References: <4F9FBE76020000AC00010179@nodcdmg2.no.trinity-health.org> Message-ID: <8D7C2D242DBD45498006B21122072BF8B477EBA5@MCINFRWEM003.ucsfmedicalcenter.org> One issue to consider is that you need to find a way to compare apples to apples. Generally that means doing workload by comparing identical shifts with identical work practice, or doing your workload recording in small increments so you are only measuring one task at a time. For instance, while several people may be scheduled to "section" they may also be assigned additional work like changing processors, backing up special stains, doing the daily rush biopsies, doing some data entry. Along with all that the "sectioning" should be the same kinds of cases - bx vs bx, etc. All those can skew the "sectioning" totals in various ways. You need to "normalize" the counts so they are all measuring the same work in the same way. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, May 01, 2012 8:44 AM To: Allison D Scott; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Productivity Reports Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "Scott, Allison D" 04/30/2012 4:24 PM >>> >>> Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Tue May 1 11:20:05 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Tue May 1 11:20:54 2012 Subject: [Histonet] Productivity ReportsMarcia Funk In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B477EBA5@MCINFRWEM003.ucsfmedicalcenter.org> References: <4F9FBE76020000AC00010179@nodcdmg2.no.trinity-health.org> <8D7C2D242DBD45498006B21122072BF8B477EBA5@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <3328693C43A557458850CC37CE16CD1877954A2C@chw-msg-829.chw.edu> I have been battling this for years they gives us RVU's for each task. What a headache. The problem I run into is they only see per specimen. If I have a specimen that has 30 cassettes it does not matter it is treated the same as one cassette per specimen. Not to mention the doctors will hold cases for the next day or two and then one day I get slammed with a 120 cassettes and only have maybe 30-40 specimens from the regular surgical from the day before. You have to make them realize that this it is just not as cut and dry as it looks. I do not like when they just look at the numbers and graphs and not the real workload. I am the Chief Cook and bottle washer here and they still get on me about productivity. I agree with Tim on the labs doing things differently and different specimens so it is hard to compare. I feel your pain. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, May 01, 2012 9:01 AM To: Marcia Funk; Allison D Scott; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Productivity ReportsMarcia Funk One issue to consider is that you need to find a way to compare apples to apples. Generally that means doing workload by comparing identical shifts with identical work practice, or doing your workload recording in small increments so you are only measuring one task at a time. For instance, while several people may be scheduled to "section" they may also be assigned additional work like changing processors, backing up special stains, doing the daily rush biopsies, doing some data entry. Along with all that the "sectioning" should be the same kinds of cases - bx vs bx, etc. All those can skew the "sectioning" totals in various ways. You need to "normalize" the counts so they are all measuring the same work in the same way. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, May 01, 2012 8:44 AM To: Allison D Scott; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Productivity Reports Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "Scott, Allison D" 04/30/2012 4:24 PM >>> >>> Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aferullo <@t> celgene.com Tue May 1 11:53:13 2012 From: aferullo <@t> celgene.com (Andrea Ferullo (non-Celgene)) Date: Tue May 1 11:53:17 2012 Subject: [Histonet] Frozen brains on cryostat Message-ID: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Hello everyone, I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT. I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up. I would appreciate any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. Thanks and I look forward to your advice. Andrea ********************************************************* THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR INDIVIDUALS NAMED ABOVE. If the reader is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please reply to the sender to notify us of the error and delete the original message. Thank You. ********************************************************* From talulahgosh <@t> gmail.com Tue May 1 12:12:20 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue May 1 12:12:23 2012 Subject: [Histonet] Frozen brains on cryostat In-Reply-To: References: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Message-ID: If they haven't been fixed, good luck! I've never been able to section unfixed tissue. Then again, I work with embryonic tissue only. Have you tried sectioning at a smaller section size? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < aferullo@celgene.com> wrote: > Hello everyone, > > I recently received rat brain samples that were frozen in liquid nitrogen > and embedded in OCT. I sectioned them on the cryostat and they are coming > out very wrinkled, no matter what technique I use to pick them up. I would > appreciate any tips/tricks that anyone has to offer. Forebrain sections > are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. > Thanks and I look forward to your advice. > > Andrea > > ********************************************************* > THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS > CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED > INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL > OR INDIVIDUALS NAMED ABOVE. > If the reader is not the intended recipient, or the > employee or agent responsible to deliver it to the > intended recipient, you are hereby notified that any > dissemination, distribution or copying of this > communication is strictly prohibited. If you have > received this communication in error, please reply to the > sender to notify us of the error and delete the original > message. Thank You. > ********************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Tue May 1 13:24:22 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue May 1 13:24:55 2012 Subject: [Histonet] Frozen brains on cryostat In-Reply-To: References: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Message-ID: <1FFD6B41-7550-4E0D-9588-C7C8116F2F26@email.arizona.edu> Aggghhhhhhhhh! Cryosectioning brains! There are many tricks and I have just learned after 13 years of struggling with them a few of the tricks. Most of the brains I have to section are rodent and they have been fixed and sucrose protected. Sometimes they are in OCT and other times not. First trick - keep the slides in the cryostat so they are cold. Cut your section and tamp out any wrinkles or folds (or as many as possible). Then put a slide on top of the section and hopefully it will stick. Then very carefully rub a warm finger on the back of the slide to melt and further attach the section to the slide. Start at one end and move down over the rest of the tissue. This should get you a wrinkle and bubble free section. Somebody on histonet suggested this about a year ago and I was tired of pulling out my hair trying to get good frozen brain sections so I tried it and IT WORKED!!!!! You might be able to find the original post in the histonet archives. Andi G. On May 1, 2012, at 10:12 AM, Emily Sours wrote: > If they haven't been fixed, good luck! I've never been able to section > unfixed tissue. > Then again, I work with embryonic tissue only. > Have you tried sectioning at a smaller section size? > > Emily > > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > > On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < > aferullo@celgene.com> wrote: > >> Hello everyone, >> >> I recently received rat brain samples that were frozen in liquid nitrogen >> and embedded in OCT. I sectioned them on the cryostat and they are coming >> out very wrinkled, no matter what technique I use to pick them up. I would >> appreciate any tips/tricks that anyone has to offer. Forebrain sections >> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. >> Thanks and I look forward to your advice. >> >> Andrea >> >> ********************************************************* >> THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS >> CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED >> INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL >> OR INDIVIDUALS NAMED ABOVE. >> If the reader is not the intended recipient, or the >> employee or agent responsible to deliver it to the >> intended recipient, you are hereby notified that any >> dissemination, distribution or copying of this >> communication is strictly prohibited. If you have >> received this communication in error, please reply to the >> sender to notify us of the error and delete the original >> message. Thank You. >> ********************************************************* >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bboyce <@t> NEMOURS.ORG Tue May 1 13:27:28 2012 From: bboyce <@t> NEMOURS.ORG (Boyce, Bobbie) Date: Tue May 1 13:27:55 2012 Subject: [Histonet] Frozen brains on cryostat In-Reply-To: References: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Message-ID: Hi Andrea, I'm not sure about the unfixed tissue, but we got awesome advice from Donna Emge in regards to our wrinkle problems on fixed frozen brains. We would dip a brush in water and run that across the slide (not too much and not too little), then pick up the tissue. My first thought was, "Water in the cryo?", but it really worked. Good luck! Bobbie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, May 01, 2012 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen brains on cryostat If they haven't been fixed, good luck! I've never been able to section unfixed tissue. Then again, I work with embryonic tissue only. Have you tried sectioning at a smaller section size? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < aferullo@celgene.com> wrote: > Hello everyone, > > I recently received rat brain samples that were frozen in liquid nitrogen > and embedded in OCT. I sectioned them on the cryostat and they are coming > out very wrinkled, no matter what technique I use to pick them up. I would > appreciate any tips/tricks that anyone has to offer. Forebrain sections > are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. > Thanks and I look forward to your advice. > > Andrea > > ********************************************************* > THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS > CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED > INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL > OR INDIVIDUALS NAMED ABOVE. > If the reader is not the intended recipient, or the > employee or agent responsible to deliver it to the > intended recipient, you are hereby notified that any > dissemination, distribution or copying of this > communication is strictly prohibited. If you have > received this communication in error, please reply to the > sender to notify us of the error and delete the original > message. Thank You. > ********************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBEDRI <@t> PARTNERS.ORG Tue May 1 13:59:10 2012 From: BBEDRI <@t> PARTNERS.ORG (Bedri, Babiker) Date: Tue May 1 13:59:52 2012 Subject: [Histonet] RE:. Cassette printer comparison In-Reply-To: References: Message-ID: <2C0DC30544A81949A1A741F7087245AE0EBF58D1@PHSX10MB5.partners.org> We have used the General Data cassette printers for 6 plus years now, both 1 and 2 D barcodes. We have both the 12 hopper (magazine) and single hoper printers. The single hoppers are faster but require manual change of hoppers if you are using different colored cassettes. The quality of the print is excellent, reliable on scanning and can withstand a lot of punishment, believe me we tried. You must have the under counter space for the Hepa filter. Babiker Bedri MGH Pathology Boston, MA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, May 01, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 102, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. blocks processed in plastic (Nancy Schmitt) 2. RE: blocks processed in plastic (Jack Ratliff) 3. Cassette printer comparison (Morken, Timothy) 4. RE: Cassette printer comparison (Davide Costanzo) 5. Productivity Reports (Scott, Allison D) 6. Re: Productivity Reports (Rene J Buesa) 7. Part Time MOHS Tech Needed ASAP (Melissa Phelan) 8. Re: Productivity Reports (Marcia Funk) 9. RE: Productivity Reports (Morken, Timothy) 10. RE: Productivity ReportsMarcia Funk (Heckford, Karen - SMMC-SF) 11. Frozen brains on cryostat (Andrea Ferullo (non-Celgene)) ---------------------------------------------------------------------- Message: 1 Date: Mon, 30 Apr 2012 17:04:22 +0000 From: Nancy Schmitt Subject: [Histonet] blocks processed in plastic To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36DEE18B@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters- Is there a special blade or angle or directions required for cutting plastic blocks? As always - Thanks for your help Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 2 Date: Mon, 30 Apr 2012 14:43:18 -0400 From: Jack Ratliff Subject: RE: [Histonet] blocks processed in plastic To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Nancy! What type of plastic blocks are you wanting to cut? May I also ask what type of specimens are contained within the blocks. Without much information on what specifically you are working with you are looking at needing diamond knives, tungsten-carbide knives, or diamond impregnated wire, band saw blades, or disc wheels. Best, Jack Jack Ratliff Senior Histologist, BioMimetic Therapeutics, Inc. Chairman, Hard Tissue Committee - National Society for Histotechnology > From: Nancy_Schmitt@pa-ucl.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 30 Apr 2012 17:04:22 +0000 > Subject: [Histonet] blocks processed in plastic > > Hi Histonetters- > Is there a special blade or angle or directions required for cutting plastic blocks? > As always - Thanks for your help > Nancy > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 30 Apr 2012 12:18:46 -0700 From: "Morken, Timothy" Subject: [Histonet] Cassette printer comparison To: Histonet Message-ID: <8D7C2D242DBD45498006B21122072BF8B477E509@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Hi all, We are looking at cassette printers and have narrowed to three. The printers will be used for 2D code printing so I am wondering what experience anyone has had with reliability of 2D code readability and durability with the output of these three printers Thermo Printmate General Data Leica IP-C Thanks for any info you can give! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org ------------------------------ Message: 4 Date: Mon, 30 Apr 2012 13:09:17 -0700 From: Davide Costanzo Subject: RE: [Histonet] Cassette printer comparison To: "Morken, Timothy" , Histonet Message-ID: <-3998100205982320642@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 The General Data is nice, but takes up space with its vacuum canister, which they rarely show in pictures. Be sure you see it and make certain it will fit in the space you have. Cassettes are more costly as they are all pre-printed with black facing, but look great. Thermo is less bulky (no vacuum and waste container to clean) but produces a lower quality print. Cassettes are much cheaper, but they have had some troubles with the print head not lasting long. The Leica is not much better (if at all) than the Thermo with respect to print quality. It also constantly jams (the ones I have used). Personally, I would choose the General Data. Costs more in the long term with pricey cassettes, but looks great! Less troubles with barcode scanning too. There is no comparison between this and the other two. Like a Kia vs a Bentley. I just bought the Thermo, and only because my lab is a Thermo Showcase lab and that is all we use, across the board. I wish I could have the General Data unit. Sent from my Windows Phone From: Morken, Timothy Sent: 4/30/2012 12:19 PM To: Histonet Subject: [Histonet] Cassette printer comparison Hi all, We are looking at cassette printers and have narrowed to three. The printers will be used for 2D code printing so I am wondering what experience anyone has had with reliability of 2D code readability and durability with the output of these three printers Thermo Printmate General Data Leica IP-C Thanks for any info you can give! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 30 Apr 2012 21:24:41 +0000 From: "Scott, Allison D" Subject: [Histonet] Productivity Reports To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 6 Date: Tue, 1 May 2012 04:53:28 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Productivity Reports To: "histonet@lists.utsouthwestern.edu" , Allison DScott Message-ID: <1335873208.39081.YahooMailClassic@web162105.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Productivity figures are fundamental to justify the staff you have or the one you will need if a work growth is expected or planned by your director. There are many ways of figuring out productivity and not all tasks can be measured in the same way. For instance: 1- FS productivity ought to be measured in the time (minutes) elapsing between the moment the specimen reached the FS area and the moment the histotech (HT)?delivers to the pathologist (PT) the slides ready to read. By the way, this is the only task that CAP has developed a productivity standard and it is 20 minutes and the PT has to justify if any FS takes more than the required?20 minutes. As to the HT, the average productivity if 15 minutes. 2- For the lab supervisor, two productivity indices are casted blocks per hour, while embedding or blocks per hour while sectioning, and the list can be expanded to other tasks where the work unit is the block. 3- all in all, the Canadian model of measuring productivity, that I have included in my calculations, refers to the number of blocks (or cases in other occasions) divided by the?total time it elapses between the moment the case is received at the lab. until the diagnosis is sent to the physician. That would be the Total Gross Work flow productivity. ? There are many ways but what is absolutely certain is that if you want to have a grasp of your lab and staff and control their activities, as well as analyze the whole operation to improve the work flow, you need to determine productivity. There are always those that, with reason, argue that excessive productivity can "damage" quality. The argument seems to be correct in general but in no way it can deter trying to be as productive as possible and maintain quality. There should be no compromises either way. An "ideal" quality cannot affect productivity, and viceversa. Under separate cover I am sending 2 works I published on this issue. You should show them to your boss and let him/her select which productivity indices s/he prefers. Ren? J. --- On Mon, 4/30/12, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Productivity Reports To: "histonet@lists.utsouthwestern.edu" Date: Monday, April 30, 2012, 5:24 PM Hello to all in histoland.? Does any one have to do productivity reports for their boss.? If so, what are you using for your unit of measure to say if your techs are being productive.? My boss is really into the numbers, graphs and charts.? I have a my techs fill out a workload recording form.? I really don't know where to begin trying to pull data from it for a report.? If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 01 May 2012 09:42:25 -0400 From: Melissa Phelan Subject: [Histonet] Part Time MOHS Tech Needed ASAP To: Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Allied Search Partners is working with a small laboratory who is in need of a Part Time MOHS Histotech. This position is an immediate need. We are looking for someone who has experience in doing MOHS Histology. To apply: Please send resume to Melissa@alliedsearchpartners.com or fax to 888 388 7572. Please note: No other information is disclosed about the organization until we receive your resume for review. All qualified applicants will be contacted with further information prior to an interview being scheduled for the position. Thank you! Location(s): Mt. Pleasant, WI 3 or 4 days per week and 1?2 a Day in Franklin, WI Schedule: Monday-Friday Part Time Hours. This position has the potential to become a full time position. Travel: Majority of the work is in Mt. Pleasant, WI area and 1?2 a Day in Franklin, WI area. Requirements: Ability to travel between two locations on one day per week MOHS Histology Experience Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com ------------------------------ Message: 8 Date: Tue, 01 May 2012 11:44:06 -0400 From: "Marcia Funk" Subject: Re: [Histonet] Productivity Reports To: "Allison D Scott" , "histonet@lists.utsouthwestern.edu" Message-ID: <4F9FBE76020000AC00010179@nodcdmg2.no.trinity-health.org> Content-Type: text/plain; charset=US-ASCII Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "Scott, Allison D" 04/30/2012 4:24 PM >>> Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 1 May 2012 09:01:24 -0700 From: "Morken, Timothy" Subject: RE: [Histonet] Productivity Reports To: "Marcia Funk" , "Allison D Scott" , "histonet@lists.utsouthwestern.edu" Message-ID: <8D7C2D242DBD45498006B21122072BF8B477EBA5@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii One issue to consider is that you need to find a way to compare apples to apples. Generally that means doing workload by comparing identical shifts with identical work practice, or doing your workload recording in small increments so you are only measuring one task at a time. For instance, while several people may be scheduled to "section" they may also be assigned additional work like changing processors, backing up special stains, doing the daily rush biopsies, doing some data entry. Along with all that the "sectioning" should be the same kinds of cases - bx vs bx, etc. All those can skew the "sectioning" totals in various ways. You need to "normalize" the counts so they are all measuring the same work in the same way. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, May 01, 2012 8:44 AM To: Allison D Scott; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Productivity Reports Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "Scott, Allison D" 04/30/2012 4:24 PM >>> >>> Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 1 May 2012 09:20:05 -0700 From: "Heckford, Karen - SMMC-SF" Subject: RE: [Histonet] Productivity ReportsMarcia Funk To: "'Morken, Timothy'" , Marcia Funk , Allison D Scott , "histonet@lists.utsouthwestern.edu" Message-ID: <3328693C43A557458850CC37CE16CD1877954A2C@chw-msg-829.chw.edu> Content-Type: text/plain; charset="us-ascii" I have been battling this for years they gives us RVU's for each task. What a headache. The problem I run into is they only see per specimen. If I have a specimen that has 30 cassettes it does not matter it is treated the same as one cassette per specimen. Not to mention the doctors will hold cases for the next day or two and then one day I get slammed with a 120 cassettes and only have maybe 30-40 specimens from the regular surgical from the day before. You have to make them realize that this it is just not as cut and dry as it looks. I do not like when they just look at the numbers and graphs and not the real workload. I am the Chief Cook and bottle washer here and they still get on me about productivity. I agree with Tim on the labs doing things differently and different specimens so it is hard to compare. I feel your pain. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, May 01, 2012 9:01 AM To: Marcia Funk; Allison D Scott; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Productivity ReportsMarcia Funk One issue to consider is that you need to find a way to compare apples to apples. Generally that means doing workload by comparing identical shifts with identical work practice, or doing your workload recording in small increments so you are only measuring one task at a time. For instance, while several people may be scheduled to "section" they may also be assigned additional work like changing processors, backing up special stains, doing the daily rush biopsies, doing some data entry. Along with all that the "sectioning" should be the same kinds of cases - bx vs bx, etc. All those can skew the "sectioning" totals in various ways. You need to "normalize" the counts so they are all measuring the same work in the same way. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, May 01, 2012 8:44 AM To: Allison D Scott; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Productivity Reports Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "Scott, Allison D" 04/30/2012 4:24 PM >>> >>> Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 1 May 2012 12:53:13 -0400 From: "Andrea Ferullo (non-Celgene)" Subject: [Histonet] Frozen brains on cryostat To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Content-Type: text/plain; charset="us-ascii" Hello everyone, I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT. I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up. I would appreciate any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. Thanks and I look forward to your advice. Andrea ********************************************************* THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR INDIVIDUALS NAMED ABOVE. If the reader is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please reply to the sender to notify us of the error and delete the original message. Thank You. ********************************************************* ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 102, Issue 1 **************************************** The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue May 1 15:38:37 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue May 1 15:38:42 2012 Subject: [Histonet] Integrin Antibody Message-ID: I am attempting to work up alpha2 beta 1 intergrin from abcam. I have tried lots of things, But if anyone has any suggestions to get it to work I would appreciate it. Even if it means getting a different antibody. thank you. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From Diane.Tokugawa <@t> kp.org Tue May 1 18:01:36 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Tue May 1 18:01:57 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 04/30/2012 and will not return until 05/02/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From gonavy2003 <@t> gmail.com Wed May 2 02:13:35 2012 From: gonavy2003 <@t> gmail.com (Rick Tiefenauer) Date: Wed May 2 02:13:41 2012 Subject: [Histonet] Productivity Reports Message-ID: Allison, One KEY to success I have found is to make sure your boss knows what he or she wants, and get an agreement on what you need to report before you start. It can be very frustrating to invest resources in reporting what isn't value added. My problem has always been, that determining ACTUAL productivity reaches outside of the lab if it is to be used to determine something like the labs overall fiscal contribution to the enterprise. If it is simply to measure "turn around time" for patient cases, that can become a complicated set of time measurements from point to point within the lab, or it can be as simple as IN and OUT, total elapsed time. What works for you should be driven by what is to be accomplished with the productivity report. There are 4 questions I would ask immediately 1. What will the data be used for? (want it to accomplish) 2. What investment is willing to be made in capturing and maintaining data? (cost) 3. Is this a ?one-time? measurement? (if this is the case, maybe an informed and educated calculation can be made using existing data. Work flow, billed hours, and patient tracking should point to productivity, however this will have a higher error factor than a measurement controlled within the lab. Payroll, patient billing, or IT should be able to provide this data.) 4. Is this a paradigm shift to capture data on a continuous basis to perform trend analysis to improve the flow of work in an attempt to incorporate six-sigma/lean principles in the lab? (if so, a six-sigma black belt would be a good consultant) Or, again depending on the detail of existing data mentioned earlier, you might be able to set up an ad-hoc report that is issued on a time period that fits your schedule. An error factor can be established and reported with the data each time. MS Excel makes great charts and graphs. This would be an easy way to report specific data applicable to your lab only. I am new to the Histotech field but I have over 20 years in Quality Assurance, I can tell you from experience that sometimes there is more effort put into keeping statistics than they appear to be worth. But, what the boss wants the boss gets. One of my past supervisors used to always say, "KEEP IT SIMPLE", and make sure your metrics are *S.M.A.R.T.* *S*pecific *M*easurable *A*ttainable *R*ealistic *T*imely Best of Luck, Rick T. From Amanda <@t> YPII.com Wed May 2 04:58:54 2012 From: Amanda <@t> YPII.com (Mandy O'Connor) Date: Wed May 2 04:59:03 2012 Subject: [Histonet] CAP inspection requirements Message-ID: <8287D830EC5C904AA3E482C75773A03B0A118FBB@EX1.YPIILab.YPII.com> What are the requirements to become a CAP inspector? Do you have to at least be an actual registered HT/HTL? Is just having a 2 year histotech degree enough? Thanks, Mandy O'Connor, HTL (ASCP) Yellowstone Pathology Instit. Billings, MT From one_angel_secret <@t> yahoo.com Wed May 2 06:46:19 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed May 2 06:46:31 2012 Subject: [Histonet] CAP inspection requirements In-Reply-To: <8287D830EC5C904AA3E482C75773A03B0A118FBB@EX1.YPIILab.YPII.com> References: <8287D830EC5C904AA3E482C75773A03B0A118FBB@EX1.YPIILab.YPII.com> Message-ID: <9F97AB21-AB61-4BBB-B83A-4CE5637744C1@yahoo.com> CAP inspectors are your peers. In other words they are people managing other labs. So those people have a variety of degrees or experiences. They don't get paid to inspect. It's a requirement that labs that participate in CAP bi annually inspect another lab because CAP works as a peer review system. I'm sure there are actual paid jobs with CAP but best to look at Their website for those opportunity for job descriptions. Hope this helps. Kim D Sent from my iPhone On May 2, 2012, at 5:58 AM, Mandy O'Connor wrote: > What are the requirements to become a CAP inspector? Do you have to at least be an actual registered HT/HTL? Is just having a 2 year histotech degree enough? > > Thanks, > Mandy O'Connor, HTL (ASCP) > Yellowstone Pathology Instit. > Billings, MT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Wed May 2 07:21:56 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed May 2 07:22:07 2012 Subject: [Histonet] DeCloaking Chamber Message-ID: <3328693C43A557458850CC37CE16CD1877954A2D@chw-msg-829.chw.edu> Hi, Does anyone know of a delayed start Decloaking chamber that is independent from the IHC stainer? I am looking around to get a new Decloaking chamber. Any suggetions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From christina.thurby <@t> bms.com Wed May 2 12:42:00 2012 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Wed May 2 12:42:10 2012 Subject: [Histonet] RE: Re: Frozen brains on cryostat In-Reply-To: References: Message-ID: Andrea, A couple of suggestions for you to help with sectioning the brain tissue. If you haven't already adjusted the cryostat temperature that is a place to start. Brains section really well at -10 to -15 C. Make sure to let the blocks equilibrate to the temperature in the cryostat. If you've taken the frozen blocks out of a -70 C freezer, let them sit in the cryostat for at least 20-30 minutes to 'warm up' to the cryostat temperature. At times, I have really good luck getting nice sections when I do the following: 1. Shave (or face off) the block until you're ready to get a section. 2. Using a gloved hand, place your thumb over the tissue for a few seconds. 3. Begin sectioning - discard the first section that comes off, the second section is usually really nice (be ready to use your chilled brush) and gently pull the section toward you as you are moving the wheel (manual instrument). 4. This method can be repeated several times - just be patient. It does take some practice. Finally, if you have any trouble getting the brain sections to stay adhered to the glass slides, depending on the downstream application you may want to try using the Gold Plus Slides (I think they are from Thermo Shandon or maybe Erie Scientific??) These slides are really nice to use when working with brain tissue if you experience 'tissue lifting' during staining applications - but in our experience they can't be used for LCM work. Good Luck! Kristie Christina Thurby Bristol Myers Squibb 812-307-2093 >> On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < >> aferullo@celgene.com> wrote: >> >>> Hello everyone, >>> >>> I recently received rat brain samples that were frozen in liquid nitrogen >>> and embedded in OCT. I sectioned them on the cryostat and they are coming >>> out very wrinkled, no matter what technique I use to pick them up. I would >>> appreciate any tips/tricks that anyone has to offer. Forebrain sections >>> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. >>> Thanks and I look forward to your advice. >>> >>> Andrea This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From jsjurczak <@t> comcast.net Wed May 2 12:48:40 2012 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Wed May 2 12:48:47 2012 Subject: [Histonet] Grossing chair Message-ID: <829751199.2026664.1335980920084.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Does anybody out there have a grossing chair that they could recommend to us? We're shopping for something that's more amenable to 4 or 5 hour sessions. We're sitting at a Mopec grossing station. Thanks From cpyse <@t> x-celllab.com Wed May 2 14:10:18 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed May 2 14:10:43 2012 Subject: [Histonet] DeCloaking Chamber In-Reply-To: <3328693C43A557458850CC37CE16CD1877954A2D@chw-msg-829.chw.edu> References: <3328693C43A557458850CC37CE16CD1877954A2D@chw-msg-829.chw.edu> Message-ID: <003901cd2897$3721b0e0$a56512a0$@com> Karen I would just purchase a timer to plug the Decloaking chamber into, I use one to heat my water bath for pretreatments prior to by techs coming in. I purchased the timers from Fisher, cat. #666224 for $28.82. Works like a charm. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, May 02, 2012 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DeCloaking Chamber Hi, Does anyone know of a delayed start Decloaking chamber that is independent from the IHC stainer? I am looking around to get a new Decloaking chamber. Any suggetions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed May 2 15:02:36 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed May 2 15:02:39 2012 Subject: [Histonet] IMF antibody validation Message-ID: Hello everyone, I'm branching out into IMF antibodies and as such am starting to validate these antibodies. Previously no comparison studies were done that I know of. Now we're running frozen tissue slides manually with our current set of antibodies simultaneously with new vendors' antibodies being run automated. Since IMF is not permanent, my question to all of you is, are you photographing the slides for a permanent record or just keeping a written record of the comparison results? Any and all responses are welcome. Thanks, Linda Sebree From amosbrooks <@t> gmail.com Wed May 2 15:46:08 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 2 15:46:13 2012 Subject: [Histonet] DeCloaking Chamber Message-ID: Hi, Here are the best steamers on the market... http://www.walmart.com/ip/Black-Decker-7-Quart-Food-Steamer/14320967?findingMethod=rr There are others that will work as well. Use only one layer. The second layer is never as hot as the first. I drill a hole in the top and drop in a thermometer to monitor the temperature in real time. If you spend over $50 on such a product you are getting robbed. Happy shopping, Amos On Wed, May 2, 2012 at 1:01 PM, wrote: > Message: 10 > Date: Wed, 2 May 2012 05:21:56 -0700 > From: "Heckford, Karen - SMMC-SF" > Subject: [Histonet] DeCloaking Chamber > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3328693C43A557458850CC37CE16CD1877954A2D@chw-msg-829.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > > Hi, > Does anyone know of a delayed start Decloaking chamber that is independent > from the IHC stainer? I am looking around to get a new Decloaking chamber. > Any suggetions? > > Karen Heckford HT ASCP CE > From Courtney.Pierce <@t> quintiles.com Thu May 3 09:13:04 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Thu May 3 09:13:11 2012 Subject: [Histonet] Phosphatase Treatment Message-ID: Does anyone have any info about Phosphatase Treatment and how to use it? Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From dreynold <@t> mdanderson.org Thu May 3 09:42:27 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Thu May 3 09:42:47 2012 Subject: [Histonet] RE: Frozen brains on cryostat Message-ID: <785BBF0C5F49CE41BA74460A43A08F02315AC3F02F@DCPWVMBXC0VS3.mdanderson.edu> I cut mouse brains frozen in OCT with very little problem. I bring the Cyrostat temp up to between -15 to -13. Pick them up with a warm slide and seldom have a wrinkle. Brains are unfixed placed in OCT and frozen with liquid nitrogen. Donna Reynolds, Chief Histology Lab (IHC) U.T, M.D. Anderson Cancer Center Houston, Texas 713-792-8106 Message: 11 Date: Tue, 1 May 2012 12:53:13 -0400 From: "Andrea Ferullo (non-Celgene)" Subject: [Histonet] Frozen brains on cryostat To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Content-Type: text/plain; charset="us-ascii" Hello everyone, I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT. I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up. I would appreciate any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. Thanks and I look forward to your advice. Andrea ************ From Valerie.Hannen <@t> parrishmed.com Thu May 3 10:26:29 2012 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu May 3 10:26:37 2012 Subject: [Histonet] .._Bone Marrows Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40DF7@isexstore03> Good morning Histonetters!!! Our Pathologist has been reading again !! He read an article about Bone Marrow fixation. I have a few questions to pose to you all. 1) Do you typically fix your Bone Marrow biopsies overnight before decalcification? We currently do not. 2) What are you fixing them in? Formalin? Zinc Formalin? (this is what we currently are using) B Plus? (this was the fixative mentioned in the article). Any and all input will be greatly appreciated!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From kc <@t> ka-recruiting.com Thu May 3 10:28:35 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Thu May 3 10:28:08 2012 Subject: [Histonet] Histology jobs Message-ID: <212107475.1336058915414.JavaMail.cfservice@sl4app2> Dear Histonet Community, I hope you are all having a great week. I am a one of the founders of a nationally recognized healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in taking the next step in your career. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position with a hospital outside of Columbus, Ohio. This is a 1st shift Histotechnologist position at one of Ohio's most highly regarded hospitals. Recognized for superior patient satisfaction this 300 bed, level 1 trauma center hospital offers an excellent work environment. This is a position offers a terrific compensation package; including competitive hourly rate, relocation assistance, and retirement plan. To qualify you must have an AS or BS in Histology and be either HT or HTL (ASCP) certified. Working knowledge of Tissue Processors, Micrometers, Immunohistochemical and Special Staining Instrumentation is required. If you are interested in learning more about this position, please call or e-mail me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email with a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. Histotech Openings: * ME - Lead Surgical Pathologist * NY - Western - Histotech * NY - NYC - Histotech * NYC - Pathology Manager (commercial background) * PA - South Eastern - IHC Tech * IN - Histotech - 2nd shift * NC - Histology Supervisor 2nd shift * NC - Histology Manager * FL - Treasure Coast - Histotech * WY - Histotech - 1st shift * CO - Denver - Histotech * NV - IHC tech * CA - Southern - Histology Manager I look forward to hearing from you. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From rjbuesa <@t> yahoo.com Thu May 3 10:56:10 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 3 10:56:18 2012 Subject: [Histonet] .._Bone Marrows In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40DF7@isexstore03> Message-ID: <1336060570.19838.YahooMailClassic@web162105.mail.bf1.yahoo.com> Our?bone marrow trephines were fixed?with neutral buffered formalin from the moment they were taken to about 6 PM (from?8 to?12 hours) in NBF before placing them in EDTA overnight. The only thing is that we were absolutely sure that the pH of the NBF was 7.0 Always got very good results. Ren? J. --- On Thu, 5/3/12, Hannen, Valerie wrote: From: Hannen, Valerie Subject: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" Date: Thursday, May 3, 2012, 11:26 AM Good morning Histonetters!!! Our Pathologist has been reading again !!? He read an article about Bone Marrow fixation. I have a few questions to pose to you all. 1) Do you typically fix your Bone Marrow biopsies overnight before decalcification?? We currently do not. 2) What are you fixing them in? Formalin?? ? ? ? Zinc Formalin? (this is what we currently are using)? ? ???B Plus? (this was the fixative mentioned in the article). Any and all input will be greatly appreciated!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Thu May 3 11:19:27 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Thu May 3 11:19:35 2012 Subject: [Histonet] (no subject) Message-ID: We fix bone marrow cores in a B5 substitute before decalcification; usually a minimum of 2 hours depending upon the size of the core. ? Brendal Finlay, HT (ASCP) From anna.c.hughes <@t> gsk.com Thu May 3 12:09:53 2012 From: anna.c.hughes <@t> gsk.com (Anna Hughes) Date: Thu May 3 12:10:02 2012 Subject: [Histonet] PEN2 (PSENEN) or B7-H4 (VTCN1) Message-ID: <56B8D7633148A9419B559EBE8E9CF5903F6466@019-SN1MPN1-033.019D.MGD.MSFT.NET> Hi Everyone! Does anyone have any experience with these antibodies from any source? :PEN2 (PSENEN) or B7-H4 (VTCN1). If so, what antibodies are you using, and what are your IHC results on human tissue? Thanks for your time! Anna Anna C. Hughes anna.c.hughes@gsk.com From BBEDRI <@t> PARTNERS.ORG Thu May 3 12:21:14 2012 From: BBEDRI <@t> PARTNERS.ORG (Bedri, Babiker) Date: Thu May 3 12:21:35 2012 Subject: Subject: [Histonet] .._Bone Marrows In-Reply-To: References: Message-ID: <2C0DC30544A81949A1A741F7087245AE0EBF854E@PHSX10MB5.partners.org> Hi Valerie, We fix bone marrow cores in B-Plus for a minimum of 2 hours, rinse then decal in Rapid Cal Immuno (with agitation) for 30 minutes. Both chemicals are supplied by BBC Biochemical (1-800-635-4477) Babiker Bedri MGH Pathology Boston, MA 02114 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 03, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 102, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Re: Frozen brains on cryostat (Thurby, Christina) 2. Grossing chair (jsjurczak@comcast.net) 3. RE: DeCloaking Chamber (Cynthia Pyse) 4. IMF antibody validation (Sebree Linda A) 5. DeCloaking Chamber (Amos Brooks) 6. Phosphatase Treatment (Courtney Pierce) 7. RE: Frozen brains on cryostat (Reynolds,Donna M) 8. .._Bone Marrows (Hannen, Valerie) 9. Histology jobs (K.C. Carpenter) 10. Re: .._Bone Marrows (Rene J Buesa) 11. (no subject) (Brendal Finlay) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 May 2012 13:42:00 -0400 From: "Thurby, Christina" Subject: [Histonet] RE: Re: Frozen brains on cryostat To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Andrea, A couple of suggestions for you to help with sectioning the brain tissue. If you haven't already adjusted the cryostat temperature that is a place to start. Brains section really well at -10 to -15 C. Make sure to let the blocks equilibrate to the temperature in the cryostat. If you've taken the frozen blocks out of a -70 C freezer, let them sit in the cryostat for at least 20-30 minutes to 'warm up' to the cryostat temperature. At times, I have really good luck getting nice sections when I do the following: 1. Shave (or face off) the block until you're ready to get a section. 2. Using a gloved hand, place your thumb over the tissue for a few seconds. 3. Begin sectioning - discard the first section that comes off, the second section is usually really nice (be ready to use your chilled brush) and gently pull the section toward you as you are moving the wheel (manual instrument). 4. This method can be repeated several times - just be patient. It does take some practice. Finally, if you have any trouble getting the brain sections to stay adhered to the glass slides, depending on the downstream application you may want to try using the Gold Plus Slides (I think they are from Thermo Shandon or maybe Erie Scientific??) These slides are really nice to use when working with brain tissue if you experience 'tissue lifting' during staining applications - but in our experience they can't be used for LCM work. Good Luck! Kristie Christina Thurby Bristol Myers Squibb 812-307-2093 >> On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < >> aferullo@celgene.com> wrote: >> >>> Hello everyone, >>> >>> I recently received rat brain samples that were frozen in liquid nitrogen >>> and embedded in OCT. I sectioned them on the cryostat and they are coming >>> out very wrinkled, no matter what technique I use to pick them up. I would >>> appreciate any tips/tricks that anyone has to offer. Forebrain sections >>> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. >>> Thanks and I look forward to your advice. >>> >>> Andrea This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 2 Date: Wed, 2 May 2012 17:48:40 +0000 (UTC) From: jsjurczak@comcast.net Subject: [Histonet] Grossing chair To: histonet@lists.utsouthwestern.edu Message-ID: <829751199.2026664.1335980920084.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Does anybody out there have a grossing chair that they could recommend to us? We're shopping for something that's more amenable to 4 or 5 hour sessions. We're sitting at a Mopec grossing station. Thanks ------------------------------ Message: 3 Date: Wed, 2 May 2012 15:10:18 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] DeCloaking Chamber To: "'Heckford, Karen - SMMC-SF'" , Message-ID: <003901cd2897$3721b0e0$a56512a0$@com> Content-Type: text/plain; charset="us-ascii" Karen I would just purchase a timer to plug the Decloaking chamber into, I use one to heat my water bath for pretreatments prior to by techs coming in. I purchased the timers from Fisher, cat. #666224 for $28.82. Works like a charm. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, May 02, 2012 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DeCloaking Chamber Hi, Does anyone know of a delayed start Decloaking chamber that is independent from the IHC stainer? I am looking around to get a new Decloaking chamber. Any suggetions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 2 May 2012 15:02:36 -0500 From: "Sebree Linda A" Subject: [Histonet] IMF antibody validation To: Cc: Haack Lori A Message-ID: Content-Type: text/plain; charset="us-ascii" Hello everyone, I'm branching out into IMF antibodies and as such am starting to validate these antibodies. Previously no comparison studies were done that I know of. Now we're running frozen tissue slides manually with our current set of antibodies simultaneously with new vendors' antibodies being run automated. Since IMF is not permanent, my question to all of you is, are you photographing the slides for a permanent record or just keeping a written record of the comparison results? Any and all responses are welcome. Thanks, Linda Sebree ------------------------------ Message: 5 Date: Wed, 2 May 2012 16:46:08 -0400 From: Amos Brooks Subject: [Histonet] DeCloaking Chamber To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, Here are the best steamers on the market... http://www.walmart.com/ip/Black-Decker-7-Quart-Food-Steamer/14320967?findingMethod=rr There are others that will work as well. Use only one layer. The second layer is never as hot as the first. I drill a hole in the top and drop in a thermometer to monitor the temperature in real time. If you spend over $50 on such a product you are getting robbed. Happy shopping, Amos On Wed, May 2, 2012 at 1:01 PM, wrote: > Message: 10 > Date: Wed, 2 May 2012 05:21:56 -0700 > From: "Heckford, Karen - SMMC-SF" > Subject: [Histonet] DeCloaking Chamber > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3328693C43A557458850CC37CE16CD1877954A2D@chw-msg-829.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > > Hi, > Does anyone know of a delayed start Decloaking chamber that is independent > from the IHC stainer? I am looking around to get a new Decloaking chamber. > Any suggetions? > > Karen Heckford HT ASCP CE > ------------------------------ Message: 6 Date: Thu, 3 May 2012 10:13:04 -0400 From: Courtney Pierce Subject: [Histonet] Phosphatase Treatment To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have any info about Phosphatase Treatment and how to use it? Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ ------------------------------ Message: 7 Date: Thu, 3 May 2012 09:42:27 -0500 From: "Reynolds,Donna M" Subject: [Histonet] RE: Frozen brains on cryostat To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <785BBF0C5F49CE41BA74460A43A08F02315AC3F02F@DCPWVMBXC0VS3.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" I cut mouse brains frozen in OCT with very little problem. I bring the Cyrostat temp up to between -15 to -13. Pick them up with a warm slide and seldom have a wrinkle. Brains are unfixed placed in OCT and frozen with liquid nitrogen. Donna Reynolds, Chief Histology Lab (IHC) U.T, M.D. Anderson Cancer Center Houston, Texas 713-792-8106 Message: 11 Date: Tue, 1 May 2012 12:53:13 -0400 From: "Andrea Ferullo (non-Celgene)" Subject: [Histonet] Frozen brains on cryostat To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Content-Type: text/plain; charset="us-ascii" Hello everyone, I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT. I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up. I would appreciate any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. Thanks and I look forward to your advice. Andrea ************ ------------------------------ Message: 8 Date: Thu, 3 May 2012 11:26:29 -0400 From: "Hannen, Valerie" Subject: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40DF7@isexstore03> Content-Type: text/plain; charset="us-ascii" Good morning Histonetters!!! Our Pathologist has been reading again !! He read an article about Bone Marrow fixation. I have a few questions to pose to you all. 1) Do you typically fix your Bone Marrow biopsies overnight before decalcification? We currently do not. 2) What are you fixing them in? Formalin? Zinc Formalin? (this is what we currently are using) B Plus? (this was the fixative mentioned in the article). Any and all input will be greatly appreciated!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 9 Date: 3 May 2012 11:28:35 -0400 From: "K.C. Carpenter" Subject: [Histonet] Histology jobs To: histonet@lists.utsouthwestern.edu Message-ID: <212107475.1336058915414.JavaMail.cfservice@sl4app2> Content-Type: text/plain; charset="utf-8" Dear Histonet Community, I hope you are all having a great week. I am a one of the founders of a nationally recognized healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in taking the next step in your career. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position with a hospital outside of Columbus, Ohio. This is a 1st shift Histotechnologist position at one of Ohio's most highly regarded hospitals. Recognized for superior patient satisfaction this 300 bed, level 1 trauma center hospital offers an excellent work environment. This is a position offers a terrific compensation package; including competitive hourly rate, relocation assistance, and retirement plan. To qualify you must have an AS or BS in Histology and be either HT or HTL (ASCP) certified. Working knowledge of Tissue Processors, Micrometers, Immunohistochemical and Special Staining Instrumentation is required. If you are interested in learning more about this position, please call or e-mail me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email with a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. Histotech Openings: * ME - Lead Surgical Pathologist * NY - Western - Histotech * NY - NYC - Histotech * NYC - Pathology Manager (commercial background) * PA - South Eastern - IHC Tech * IN - Histotech - 2nd shift * NC - Histology Supervisor 2nd shift * NC - Histology Manager * FL - Treasure Coast - Histotech * WY - Histotech - 1st shift * CO - Denver - Histotech * NV - IHC tech * CA - Southern - Histology Manager I look forward to hearing from you. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 10 Date: Thu, 3 May 2012 08:56:10 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" , ValerieHannen Message-ID: <1336060570.19838.YahooMailClassic@web162105.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Our?bone marrow trephines were fixed?with neutral buffered formalin from the moment they were taken to about 6 PM (from?8 to?12 hours) in NBF before placing them in EDTA overnight. The only thing is that we were absolutely sure that the pH of the NBF was 7.0 Always got very good results. Ren? J. --- On Thu, 5/3/12, Hannen, Valerie wrote: From: Hannen, Valerie Subject: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" Date: Thursday, May 3, 2012, 11:26 AM Good morning Histonetters!!! Our Pathologist has been reading again !!? He read an article about Bone Marrow fixation. I have a few questions to pose to you all. 1) Do you typically fix your Bone Marrow biopsies overnight before decalcification?? We currently do not. 2) What are you fixing them in? Formalin?? ? ? ? Zinc Formalin? (this is what we currently are using)? ? ???B Plus? (this was the fixative mentioned in the article). Any and all input will be greatly appreciated!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 03 May 2012 11:19:27 -0500 From: "Brendal Finlay" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="utf-8" We fix bone marrow cores in a B5 substitute before decalcification; usually a minimum of 2 hours depending upon the size of the core. ?? Brendal Finlay, HT (ASCP) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 102, Issue 3 **************************************** The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Marilyn.A.Weiss <@t> kp.org Thu May 3 13:21:10 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu May 3 13:21:25 2012 Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 3 In-Reply-To: <201205031702.q43H2PFa019825@cnndcsmrp115.nndc.kp.org> References: <201205031702.q43H2PFa019825@cnndcsmrp115.nndc.kp.org> Message-ID: We us B-Plus for the biopsies and formalin for the clot in San Diego. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. histonet-request@lists.utsouthwestern.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 05/03/2012 10:02 AM Please respond to histonet@lists.utsouthwestern.edu To histonet@lists.utsouthwestern.edu cc Subject Histonet Digest, Vol 102, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Re: Frozen brains on cryostat (Thurby, Christina) 2. Grossing chair (jsjurczak@comcast.net) 3. RE: DeCloaking Chamber (Cynthia Pyse) 4. IMF antibody validation (Sebree Linda A) 5. DeCloaking Chamber (Amos Brooks) 6. Phosphatase Treatment (Courtney Pierce) 7. RE: Frozen brains on cryostat (Reynolds,Donna M) 8. .._Bone Marrows (Hannen, Valerie) 9. Histology jobs (K.C. Carpenter) 10. Re: .._Bone Marrows (Rene J Buesa) 11. (no subject) (Brendal Finlay) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 May 2012 13:42:00 -0400 From: "Thurby, Christina" Subject: [Histonet] RE: Re: Frozen brains on cryostat To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Andrea, A couple of suggestions for you to help with sectioning the brain tissue. If you haven't already adjusted the cryostat temperature that is a place to start. Brains section really well at -10 to -15 C. Make sure to let the blocks equilibrate to the temperature in the cryostat. If you've taken the frozen blocks out of a -70 C freezer, let them sit in the cryostat for at least 20-30 minutes to 'warm up' to the cryostat temperature. At times, I have really good luck getting nice sections when I do the following: 1. Shave (or face off) the block until you're ready to get a section. 2. Using a gloved hand, place your thumb over the tissue for a few seconds. 3. Begin sectioning - discard the first section that comes off, the second section is usually really nice (be ready to use your chilled brush) and gently pull the section toward you as you are moving the wheel (manual instrument). 4. This method can be repeated several times - just be patient. It does take some practice. Finally, if you have any trouble getting the brain sections to stay adhered to the glass slides, depending on the downstream application you may want to try using the Gold Plus Slides (I think they are from Thermo Shandon or maybe Erie Scientific??) These slides are really nice to use when working with brain tissue if you experience 'tissue lifting' during staining applications - but in our experience they can't be used for LCM work. Good Luck! Kristie Christina Thurby Bristol Myers Squibb 812-307-2093 >> On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < >> aferullo@celgene.com> wrote: >> >>> Hello everyone, >>> >>> I recently received rat brain samples that were frozen in liquid nitrogen >>> and embedded in OCT. I sectioned them on the cryostat and they are coming >>> out very wrinkled, no matter what technique I use to pick them up. I would >>> appreciate any tips/tricks that anyone has to offer. Forebrain sections >>> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. >>> Thanks and I look forward to your advice. >>> >>> Andrea This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 2 Date: Wed, 2 May 2012 17:48:40 +0000 (UTC) From: jsjurczak@comcast.net Subject: [Histonet] Grossing chair To: histonet@lists.utsouthwestern.edu Message-ID: <829751199.2026664.1335980920084.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Does anybody out there have a grossing chair that they could recommend to us? We're shopping for something that's more amenable to 4 or 5 hour sessions. We're sitting at a Mopec grossing station. Thanks ------------------------------ Message: 3 Date: Wed, 2 May 2012 15:10:18 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] DeCloaking Chamber To: "'Heckford, Karen - SMMC-SF'" , Message-ID: <003901cd2897$3721b0e0$a56512a0$@com> Content-Type: text/plain; charset="us-ascii" Karen I would just purchase a timer to plug the Decloaking chamber into, I use one to heat my water bath for pretreatments prior to by techs coming in. I purchased the timers from Fisher, cat. #666224 for $28.82. Works like a charm. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, May 02, 2012 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DeCloaking Chamber Hi, Does anyone know of a delayed start Decloaking chamber that is independent from the IHC stainer? I am looking around to get a new Decloaking chamber. Any suggetions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 2 May 2012 15:02:36 -0500 From: "Sebree Linda A" Subject: [Histonet] IMF antibody validation To: Cc: Haack Lori A Message-ID: Content-Type: text/plain; charset="us-ascii" Hello everyone, I'm branching out into IMF antibodies and as such am starting to validate these antibodies. Previously no comparison studies were done that I know of. Now we're running frozen tissue slides manually with our current set of antibodies simultaneously with new vendors' antibodies being run automated. Since IMF is not permanent, my question to all of you is, are you photographing the slides for a permanent record or just keeping a written record of the comparison results? Any and all responses are welcome. Thanks, Linda Sebree ------------------------------ Message: 5 Date: Wed, 2 May 2012 16:46:08 -0400 From: Amos Brooks Subject: [Histonet] DeCloaking Chamber To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, Here are the best steamers on the market... http://www.walmart.com/ip/Black-Decker-7-Quart-Food-Steamer/14320967?findingMethod=rr There are others that will work as well. Use only one layer. The second layer is never as hot as the first. I drill a hole in the top and drop in a thermometer to monitor the temperature in real time. If you spend over $50 on such a product you are getting robbed. Happy shopping, Amos On Wed, May 2, 2012 at 1:01 PM, wrote: > Message: 10 > Date: Wed, 2 May 2012 05:21:56 -0700 > From: "Heckford, Karen - SMMC-SF" > Subject: [Histonet] DeCloaking Chamber > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3328693C43A557458850CC37CE16CD1877954A2D@chw-msg-829.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > > Hi, > Does anyone know of a delayed start Decloaking chamber that is independent > from the IHC stainer? I am looking around to get a new Decloaking chamber. > Any suggetions? > > Karen Heckford HT ASCP CE > ------------------------------ Message: 6 Date: Thu, 3 May 2012 10:13:04 -0400 From: Courtney Pierce Subject: [Histonet] Phosphatase Treatment To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have any info about Phosphatase Treatment and how to use it? Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ ------------------------------ Message: 7 Date: Thu, 3 May 2012 09:42:27 -0500 From: "Reynolds,Donna M" Subject: [Histonet] RE: Frozen brains on cryostat To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <785BBF0C5F49CE41BA74460A43A08F02315AC3F02F@DCPWVMBXC0VS3.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" I cut mouse brains frozen in OCT with very little problem. I bring the Cyrostat temp up to between -15 to -13. Pick them up with a warm slide and seldom have a wrinkle. Brains are unfixed placed in OCT and frozen with liquid nitrogen. Donna Reynolds, Chief Histology Lab (IHC) U.T, M.D. Anderson Cancer Center Houston, Texas 713-792-8106 Message: 11 Date: Tue, 1 May 2012 12:53:13 -0400 From: "Andrea Ferullo (non-Celgene)" Subject: [Histonet] Frozen brains on cryostat To: "Histonet@lists.utsouthwestern.edu" Message-ID: <24701B0EF4127F4FA262751AA9674EEA029FE97ED479@SUMEXPRDMB03.celgene.com> Content-Type: text/plain; charset="us-ascii" Hello everyone, I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT. I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up. I would appreciate any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. Thanks and I look forward to your advice. Andrea ************ ------------------------------ Message: 8 Date: Thu, 3 May 2012 11:26:29 -0400 From: "Hannen, Valerie" Subject: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40DF7@isexstore03> Content-Type: text/plain; charset="us-ascii" Good morning Histonetters!!! Our Pathologist has been reading again !! He read an article about Bone Marrow fixation. I have a few questions to pose to you all. 1) Do you typically fix your Bone Marrow biopsies overnight before decalcification? We currently do not. 2) What are you fixing them in? Formalin? Zinc Formalin? (this is what we currently are using) B Plus? (this was the fixative mentioned in the article). Any and all input will be greatly appreciated!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 9 Date: 3 May 2012 11:28:35 -0400 From: "K.C. Carpenter" Subject: [Histonet] Histology jobs To: histonet@lists.utsouthwestern.edu Message-ID: <212107475.1336058915414.JavaMail.cfservice@sl4app2> Content-Type: text/plain; charset="utf-8" Dear Histonet Community, I hope you are all having a great week. I am a one of the founders of a nationally recognized healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in taking the next step in your career. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position with a hospital outside of Columbus, Ohio. This is a 1st shift Histotechnologist position at one of Ohio's most highly regarded hospitals. Recognized for superior patient satisfaction this 300 bed, level 1 trauma center hospital offers an excellent work environment. This is a position offers a terrific compensation package; including competitive hourly rate, relocation assistance, and retirement plan. To qualify you must have an AS or BS in Histology and be either HT or HTL (ASCP) certified. Working knowledge of Tissue Processors, Micrometers, Immunohistochemical and Special Staining Instrumentation is required. If you are interested in learning more about this position, please call or e-mail me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email with a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. Histotech Openings: * ME - Lead Surgical Pathologist * NY - Western - Histotech * NY - NYC - Histotech * NYC - Pathology Manager (commercial background) * PA - South Eastern - IHC Tech * IN - Histotech - 2nd shift * NC - Histology Supervisor 2nd shift * NC - Histology Manager * FL - Treasure Coast - Histotech * WY - Histotech - 1st shift * CO - Denver - Histotech * NV - IHC tech * CA - Southern - Histology Manager I look forward to hearing from you. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 10 Date: Thu, 3 May 2012 08:56:10 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" , ValerieHannen Message-ID: <1336060570.19838.YahooMailClassic@web162105.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Our bone marrow trephines were fixed with neutral buffered formalin from the moment they were taken to about 6 PM (from 8 to 12 hours) in NBF before placing them in EDTA overnight. The only thing is that we were absolutely sure that the pH of the NBF was 7.0 Always got very good results. Ren? J. --- On Thu, 5/3/12, Hannen, Valerie wrote: From: Hannen, Valerie Subject: [Histonet] .._Bone Marrows To: "histonet@lists.utsouthwestern.edu" Date: Thursday, May 3, 2012, 11:26 AM Good morning Histonetters!!! Our Pathologist has been reading again !! He read an article about Bone Marrow fixation. I have a few questions to pose to you all. 1) Do you typically fix your Bone Marrow biopsies overnight before decalcification? We currently do not. 2) What are you fixing them in? Formalin? Zinc Formalin? (this is what we currently are using) B Plus? (this was the fixative mentioned in the article). Any and all input will be greatly appreciated!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 03 May 2012 11:19:27 -0500 From: "Brendal Finlay" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="utf-8" We fix bone marrow cores in a B5 substitute before decalcification; usually a minimum of 2 hours depending upon the size of the core. ? Brendal Finlay, HT (ASCP) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 102, Issue 3 **************************************** From cpyse <@t> x-celllab.com Thu May 3 15:11:04 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu May 3 15:11:30 2012 Subject: [Histonet] vaginitis panel Message-ID: <000601cd2968$de6522c0$9b2f6840$@com> Histonetters For those of you performing vaginitis panels, how are you billing these? Do you bill for each separate test or use the combine CPT code? We are having a discussion on what is our best method of billing. Thanks in advance for the info. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From melissa <@t> alliedsearchpartners.com Thu May 3 15:34:28 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Thu May 3 15:34:38 2012 Subject: [Histonet] Histotech Job-Southwest FL Message-ID: Hi Everyone, A great position just came across my desk today in Southwest Florida. Please let me know if any Histotechs are out there who are looking for a full time position, preferably, right away! Thanks! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.html Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From d-emge <@t> northwestern.edu Thu May 3 18:34:45 2012 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Thu May 3 18:34:50 2012 Subject: [Histonet] re: Frozen brains on cryostat Message-ID: <54C3042A8564BA4AA2D355DD1D4C949C1072E918@chcspmbx2.ads.northwestern.edu> Andrea, I have had this happen periodically with fixed, sucrose cryoprotected frozen brain samples. Unlike fresh frozen brain samples they may not pick up flat and will usually be much wrinkled in the center. Our theory for such samples is that they were inadequately fixed before being placed in the sucrose solution, but I haven't tested this as the cause. What works for me is to use a brush to lightly moisten the slide with distilled water and then pick up the section. This works great. Do not over wet the slide though - just dampen it. One other thing, if they are fixed, sucrose cryoprotected samples then you will likely need a colder temperature -20 or -21C for sectioning than the usual -13 to -17 C that would be used for fresh frozen brain samples. Let us all know what ends up working best for you. Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 From mjdessoye <@t> commonwealthhealth.net Fri May 4 07:50:51 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Fri May 4 07:50:57 2012 Subject: [Histonet] IHC Validation Message-ID: Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From rjbuesa <@t> yahoo.com Fri May 4 08:07:20 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 4 08:07:29 2012 Subject: [Histonet] IHC Validation In-Reply-To: Message-ID: <1336136840.91835.YahooMailClassic@web162105.mail.bf1.yahoo.com> Hi Michael: This is what I think, for what it is worth: 1- if you and the reference lab are using different clones, that is not much of a validation, ? 2- additionally the validation?using another lab poses additional problems: it is not only the instrument they are using, but how they use it, what is the whole protocol before using the instrument, and how the instrument is maintained. At the end of the day, you will compare your results with those of the other lab, but qualitatively only. ? 3- now, and this is much more important: if every time you are going to implement a new antibody you are going to go through this whole validation process it will be very expensive and, as you wrote, your pathologists are satisfied. ? This is how I used to approach this issue: when I changed from manual to the DAKO auto stainer, the pathologists were the ones who decided if the results were comparable using the same controls I used manually and automated. ? Each time they wanted a new antibody or clone to be added to our battery of Abs I tried several supposedly positive controls and the pathologists either accepted or required either increasing or decreasing the Abs dilution to get the intensity and reaction pattern they were looking for when comparing with the reference they read. They (and I) also compared the results with what the literature described as desirable. ? My validations always rested on the acceptance or rejection from our pathologists. They are the ones who are going to use it and no matter who is going to "validate" your protocol, the bottom line resides with the likes or dislikes of the pathologists. You will never be able to over-rule their decision and they are the ones responsible for the whole lab results regarding CAP. ? My advise: rely on your pathologists and never attempt to do a costly?validation that is not going to be either appreciated or required. You can advise your pathologists but they are the "deciders" Ren? J. ? ? --- On Fri, 5/4/12, Dessoye, Michael J wrote: From: Dessoye, Michael J Subject: [Histonet] IHC Validation To: histonet@lists.utsouthwestern.edu Date: Friday, May 4, 2012, 8:50 AM Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT.? Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison.? I'm now faced with changing clones for several antibodies.? I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours.? I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this?? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone.? Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Fri May 4 08:10:36 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri May 4 08:11:36 2012 Subject: [Histonet] IHC Validation In-Reply-To: References: Message-ID: Hi, Think of yourself as a reference lab as well. You have a validated protocol for one clone already. Validate the new clone against the current. Your medical director can help you determine how many cases are sufficient. Depending on the antibody we validate anywhere from five cases to twenty. In my opinion, this in-house validation is the best way. The only variable is the clone. Your processing, staining platform, detection...all stays the same. Good luck melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From MMASSEY <@t> HHCS.org Fri May 4 09:29:38 2012 From: MMASSEY <@t> HHCS.org (Massey, Michelle) Date: Fri May 4 09:31:37 2012 Subject: [Histonet] FT Histology Position Message-ID: <4B7C0D513CF2204FBB171FD7C77B973C126D048DE0@mail2007.hhcs.org> Histology position available in Dalton, GA. Full-time; dayshift, hours may vary, no weekends, no holidays. Please visit HamiltonHealth.com and click on the Career section. Thanks, Michelle Massey, HT (ASCP) Tumor Bank Coord. | Histology Team Leader Hamilton Medical Center 1200 Memorial Drive Dalton, GA 30720 From Kristopher.Kalleberg <@t> unilever.com Fri May 4 09:58:09 2012 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Fri May 4 09:58:22 2012 Subject: [Histonet] Herovici stain Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0E0E5250@NTRSEVS30002.s3.ms.unilever.com> All, Does anyone know what is the chemistry/biology that lets the stain selectively bind mature/premature collagen? I have tried looking for any literature dealing with this topic but have come up empty. If anyone can give me any information or point me in the right direction of where to find any information behind this stain it would be greatly appreciated. Thank you all in advance. Kristopher L. Kalleberg Research Scientist Unilever R&D Skin Biosciences 40 Merritt Blvd. Trumbull, CT 06611 p 203.381.5765 f 203.381.5476 From burke.reed <@t> hmutx.com Fri May 4 10:54:10 2012 From: burke.reed <@t> hmutx.com (burke) Date: Fri May 4 10:54:18 2012 Subject: [Histonet] IHC Validation In-Reply-To: Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri May 4 11:02:12 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 4 11:02:19 2012 Subject: [Histonet] RE: IHC Validation In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF8B4ABC7FD@MCINFRWEM003.ucsfmedicalcenter.org> All you need to do is compare the new clone to your current clone and show the new clone is concordant with the new clone for sensitivity and specificity . You don't need to compare to the same clone elsewhere. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 5:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Fri May 4 11:04:35 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Fri May 4 11:05:19 2012 Subject: [Histonet] RE: IHC Validation In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B4ABC7FD@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Thank you all for the info. I agree that in-house validation is the way to go. The only reason we had been using an outside reference lab was due to recommendations during a JC inspection which were applied to our area, but not really meant for IHC. The inspector was not a histology person, so it was a little difficult to get our point across. But they were adamant that with only one instrument the slides should be sent out for comparison. Thanks everyone! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Friday, May 04, 2012 12:02 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: IHC Validation All you need to do is compare the new clone to your current clone and show the new clone is concordant with the new clone for sensitivity and specificity . You don't need to compare to the same clone elsewhere. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 5:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From Timothy.Morken <@t> ucsfmedctr.org Fri May 4 11:09:39 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 4 11:09:49 2012 Subject: [Histonet] RE: IHC Validation In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF8B4ABC7FD@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <8D7C2D242DBD45498006B21122072BF8B4ABC816@MCINFRWEM003.ucsfmedicalcenter.org> Outside reference (any other lab using an antibody to the same target) are helpful for those antibodies/cases for which you have scant material and may need more data. Then you can send your slides to them for staining, and stain some of their slides, and maybe trade controls. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 9:05 AM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC Validation Thank you all for the info. I agree that in-house validation is the way to go. The only reason we had been using an outside reference lab was due to recommendations during a JC inspection which were applied to our area, but not really meant for IHC. The inspector was not a histology person, so it was a little difficult to get our point across. But they were adamant that with only one instrument the slides should be sent out for comparison. Thanks everyone! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Friday, May 04, 2012 12:02 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: IHC Validation All you need to do is compare the new clone to your current clone and show the new clone is concordant with the new clone for sensitivity and specificity . You don't need to compare to the same clone elsewhere. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 5:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> cbiolabs.com Fri May 4 11:18:50 2012 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Fri May 4 11:18:56 2012 Subject: [Histonet] GI fix Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C9A534257@cbiolabs05.CBiolabs.local> Hi histoneters, Does anyone have experience with IHC on samples fixed with GI Fix from BBC? Any differences from routine NBF? Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From sadey <@t> hotmail.ca Fri May 4 11:24:48 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Fri May 4 11:24:52 2012 Subject: [Histonet] Kevlar gloves Message-ID: Hello: I'm looking to purchase some gloves that are cut resistant during autopsy cases. Can anyone recommend a brand? Thanks Sheila From timothy.m.coskran <@t> pfizer.com Fri May 4 11:54:57 2012 From: timothy.m.coskran <@t> pfizer.com (Coskran, Timothy M) Date: Fri May 4 11:55:02 2012 Subject: [Histonet] bone marrow aspirate Message-ID: <70249E5B79AFEB48A47D78568CE216E9027FB1@NDHAMREXDE02.amer.pfizer.com> Does anyone have a protocol on how to fix and process a bone marrow aspirate to paraffin? Thanks, Tim Coskran Pfizer From mtitford <@t> aol.com Fri May 4 12:04:31 2012 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri May 4 12:04:37 2012 Subject: [Histonet] Cut proof gloves Message-ID: <8CEF82F1B453650-43C-140B0@webmail-m127.sysops.aol.com> Sheila asks about cut-resistant gloves: We use the cut resistant gloves from Surgipath Medical Industries. They are of course, cut resistant, but not cut proof or puncture proof. They fit nice and snugly and don't impede work. They work well if you just touch a finger lightly with a scalpel or blade during an autopsy or while grossing a specimen. When that happens the rubber glove on top gets cut and lets the blood in and you must wash the cut resistant glove (and your hand very well of course). I don't know if they are Kevlar. That may be a brand name. Michael Titford USA Pathology Mobile AL USA From mpence <@t> grhs.net Fri May 4 12:15:28 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Fri May 4 12:15:32 2012 Subject: [Histonet] GI fix In-Reply-To: <77BC2EEB6AC66C49AEF794DC98BE314C9A534257@cbiolabs05.CBiolabs.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E20@is-e2k3.grhs.net> I have used GI Fix for years and have never had IHC problems. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anatoli Gleiberman Sent: Friday, May 04, 2012 11:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI fix Hi histoneters, Does anyone have experience with IHC on samples fixed with GI Fix from BBC? Any differences from routine NBF? Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri May 4 13:28:29 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri May 4 13:28:49 2012 Subject: [Histonet] I will be out the afternoon of 5/4/2012 and be back early Tuesday morning Message-ID: I will be out of the office starting 05/04/2012 and will not return until 05/08/2012. I will be at a inspection . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. If this is concerning a block, please call the department at 619-528-6801 or tie line 8-280-6801 Thank you. From Ashley.Troutman <@t> Vanderbilt.Edu Fri May 4 16:24:05 2012 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri May 4 16:24:16 2012 Subject: [Histonet] bone marrow aspirate Message-ID: <7B310892042DA74CB3590053F424CFE6145CB28982@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Tim, The best way I have found over the years actually requires the person collecting the specimen to do the most work. What we used to do is after the aspirate is performed, make all of the smears, and then inject the remaining aspirate directly into formalin before it coagulates. This gets rid of all the blood and ensures all that is left is marrow. After sufficient time in formalin, filter the marrow out of the formalin and process. As for a processing protocol, we do a run as follows: Formalin 30 min 70% Alcohol 20 min 90% Alcohol 10 min 100% Alcohol 10 min 100% Alcohol 10 min 100% Alcohol 15 min Xylene1 15 min Xylene2 15 min Xylene3 20 min Paraffin1 15 min Paraffin2 15 min Paraffin3 30 min This protocol was done with pressure/vacuum. We have excellent results with this and the pathologists do not have to spend a lot of time hunting for small areas of marrow on the slide, the whole slide is marrow. Good luck! Ashley Message: 5 Date: Fri, 4 May 2012 16:54:57 +0000 From: "Coskran, Timothy M" > Subject: [Histonet] bone marrow aspirate To: "histonet@lists.utsouthwestern.edu" > Message-ID: <70249E5B79AFEB48A47D78568CE216E9027FB1@NDHAMREXDE02.amer.pfizer.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a protocol on how to fix and process a bone marrow aspirate to paraffin? Thanks, Tim Coskran Pfizer From mehlikafaire <@t> hotmail.com Fri May 4 16:54:21 2012 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Fri May 4 16:54:25 2012 Subject: [Histonet] Apoptosis Antibody Message-ID: Does anyone know of a good anti-apoptotic antibody that is not made in rabbit? Preferably one that works well in immunocytochemistry or frozen sections. Thanks,Mehlika From one_angel_secret <@t> yahoo.com Fri May 4 17:30:18 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri May 4 17:30:25 2012 Subject: [Histonet] vaginitis panel In-Reply-To: <000601cd2968$de6522c0$9b2f6840$@com> References: <000601cd2968$de6522c0$9b2f6840$@com> Message-ID: It's Friday :) Noticed none touched this? Sent from my iPhone On May 3, 2012, at 4:11 PM, "Cynthia Pyse" wrote: > Histonetters > > For those of you performing vaginitis panels, how are you billing these? Do > you bill for each separate test or use the combine CPT code? We are having a > discussion on what is our best method of billing. Thanks in advance for the > info. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Sat May 5 09:59:40 2012 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Sat May 5 09:59:46 2012 Subject: [Histonet] Neutrophil Antibodies Message-ID: <5A605CE38EECB64B94485C02125A0C44300E14389F@LN-MAIL07.ln.burnham.org> Hi All in Histoland, Problem solving never ends! I am working up two antibodies for neutrophils which are NIMP-R14 from Abcam which it is a rat monoclonal and also Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have tried different conditions without any luck and was wondering if anyone has worked with these antibodies either from Abcam or another supplier and might have working conditions that have given results in the past. We are trying this on FFPE sections which the antibodies say they work on but I am thinking that I may try frozens. I was trying to avoid this however because I am using these antibodies on mouse WAT. Any help would be appreciated. Thanks!!! Happy Monday morning!!! Kind Regards! John J Shelley From liz <@t> premierlab.com Sat May 5 10:08:26 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Sat May 5 10:09:28 2012 Subject: [Histonet] RE: Neutrophil Antibodies In-Reply-To: <5A605CE38EECB64B94485C02125A0C44300E14389F@LN-MAIL07.ln.burnham.org> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5D44@SBS2K8.premierlab.local> Serotec has a really nice rat anti-mouse neutrophil antibody that works great in paraffin sections. Catalog number: MCA771 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Saturday, May 05, 2012 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutrophil Antibodies Hi All in Histoland, Problem solving never ends! I am working up two antibodies for neutrophils which are NIMP-R14 from Abcam which it is a rat monoclonal and also Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have tried different conditions without any luck and was wondering if anyone has worked with these antibodies either from Abcam or another supplier and might have working conditions that have given results in the past. We are trying this on FFPE sections which the antibodies say they work on but I am thinking that I may try frozens. I was trying to avoid this however because I am using these antibodies on mouse WAT. Any help would be appreciated. Thanks!!! Happy Monday morning!!! Kind Regards! John J Shelley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat May 5 12:14:33 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat May 5 12:14:37 2012 Subject: [Histonet] Re: bone marrow aspirate Message-ID: I don't do the procedure any more, but earlier in my career I did about 400 iliac crest aspirations and biopsies, reading them all myself. I always had an assistant, whom I often trained myself - you really can't both tend to the patient and make the smears. If the assistant didn't have her own method, I'd teach her to squirt some of the aspirate onto a slanted slide, pick up the particles with a broken applicator stick and move them to additional slides to make smears. I'd then squirt the rest of the still unclotted aspirate into neutral buffered formalin, which of course doesn't clot blood, so that you get a suspension of tiny particles in clear liquid. This could be poured into a tea bag (or nowadays into one of those little nylon bags) for further fixation and subsequent processing. In those days I'd also put the bone biopsy specimen and a clot into Zenker/Helly fixative, which of course is no more all gone. I don't see any reason to process clots today, unless the aspirate clots prematurely. Bob Richmond Samurai Pathologist Knoxville TN *********************************************** > Message: 4 > Date: Fri, 4 May 2012 16:24:05 -0500 > From: "Troutman, Kenneth A" > Subject: [Histonet] bone marrow aspirate > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<7B310892042DA74CB3590053F424CFE6145CB28982@ITS-HCWNEM06.ds.Vanderbilt.edu> > > Content-Type: text/plain; charset="us-ascii" > > Hi Tim, > > The best way I have found over the years actually requires the person collecting the specimen to do the most work. ?What we used to do is after the aspirate is performed, make all of the smears, and then inject the remaining aspirate directly into formalin before it coagulates. ?This gets rid of all the blood and ensures all that is left is marrow. ?After sufficient time in formalin, filter the marrow out of the formalin and process. > > As for a processing protocol, we do a run as follows: > Formalin 30 min > 70% Alcohol 20 min > 90% Alcohol 10 min > 100% Alcohol 10 min > 100% Alcohol 10 min > 100% Alcohol 15 min > Xylene1 15 min > Xylene2 15 min > Xylene3 20 min > Paraffin1 15 min > Paraffin2 15 min > Paraffin3 30 min > > This protocol was done with pressure/vacuum. > > We have excellent results with this and the pathologists do not have to spend a lot of time hunting for small areas of marrow on the slide, the whole slide is marrow. > > Good luck! > > Ashley > > > Message: 5 > Date: Fri, 4 May 2012 16:54:57 +0000 > From: "Coskran, Timothy M" > > Subject: [Histonet] bone marrow aspirate > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? ? ? ? ?> > Message-ID: > ? ? ? ? ? ? ? ?<70249E5B79AFEB48A47D78568CE216E9027FB1@NDHAMREXDE02.amer.pfizer.com> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have a protocol on how to fix and process a bone marrow aspirate to paraffin? > > Thanks, > Tim Coskran > Pfizer From b427297 <@t> aol.com Sat May 5 17:42:32 2012 From: b427297 <@t> aol.com (b427297@aol.com) Date: Sat May 5 17:42:46 2012 Subject: [Histonet] Neutrophil Antibodies In-Reply-To: <5A605CE38EECB64B94485C02125A0C44300E14389F@LN-MAIL07.ln.burnham.org> References: <5A605CE38EECB64B94485C02125A0C44300E14389F@LN-MAIL07.ln.burnham.org> Message-ID: <3FA30873-F986-4023-BC02-7FCB8892B0E6@aol.com> While on the topic of neuts-anyone have an antibody for canine neutrphils in ffpe? Sent from my iPhone On May 5, 2012, at 9:59 AM, John Shelley wrote: > Hi All in Histoland, > > Problem solving never ends! I am working up two antibodies for neutrophils which are NIMP-R14 from Abcam which it is a rat monoclonal and also Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have tried different conditions without any luck and was wondering if anyone has worked with these antibodies either from Abcam or another supplier and might have working conditions that have given results in the past. We are trying this on FFPE sections which the antibodies say they work on but I am thinking that I may try frozens. I was trying to avoid this however because I am using these antibodies on mouse WAT. Any help would be appreciated. Thanks!!! > > Happy Monday morning!!! > Kind Regards! > John J Shelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lab.path <@t> yahoo.com Sun May 6 10:04:50 2012 From: lab.path <@t> yahoo.com (Lab Path) Date: Sun May 6 10:07:31 2012 Subject: [Histonet] Part-time histotech needed in Milwaukee, WI Message-ID: <1336316690.79327.YahooMailNeo@web122003.mail.ne1.yahoo.com> Part-time histotech needed for small private pathology laboratory, located in downtown Milwaukee, Wisconsin. ?Hours are negotiable; they can be adjusted to the tech's schedule and availability but will probably vary somewhat with the lab's workload. Compensation is based on experience but highly competitive. Congenial work environment. No benefits. Please respond to lab.path@yahoo.com if interested. From alineumann <@t> aol.com Sun May 6 11:40:57 2012 From: alineumann <@t> aol.com (Alice Neumann) Date: Sun May 6 11:41:05 2012 Subject: [Histonet] Old Practical Reviews in Pathology CDs Message-ID: <8CEF9BE257A9135-2674-37F4D@webmail-d077.sysops.aol.com> Hello all, If anyone in a resource limited area needs these, I can mail them to you in the future on an intermittent basis. They are continuing medical education CDs in English, and I will not be able to supply a CD player or batteries, however they may be able to be used on a computer if you have a CD player on it. If you want them, please send your address and the method(s) by which you can receive mail (?Fed Ex or postal service). Best wishes, Alice Neumann MD Pinnacle Pathology PC 207 Auburn Dr. #1 Auburn, AL 36830 24 Hour Cell Phone: 307-413-4092 alineumann@aol.com From meryl50 <@t> hotmail.com Sun May 6 15:51:27 2012 From: meryl50 <@t> hotmail.com (Meryl Roberts) Date: Sun May 6 15:51:32 2012 Subject: [Histonet] how often do you change your hematoxylin? Message-ID: We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl From b427297 <@t> aol.com Sun May 6 21:39:58 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Sun May 6 21:40:09 2012 Subject: [Histonet] how often do you change your hematoxylin? In-Reply-To: References: Message-ID: <8CEFA11D4225243-1188-2CCA1@webmail-m016.sysops.aol.com> Absolutely it becomes diluted from water - are you hand staining or auto? Autostainers tend to allow a longer time for excess water to drip off - more consistency. Are you covering your htox when not in use? Do you notice any type of sheen on the surface prior to staining? Have you noticed weaker staining after 1K slides? Jackie O' -----Original Message----- From: Meryl Roberts To: histonet Sent: Sun, May 6, 2012 3:51 pm Subject: [Histonet] how often do you change your hematoxylin? We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SStephenson <@t> lifecell.com Mon May 7 06:07:49 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Mon May 7 06:09:20 2012 Subject: [Histonet] how often do you change your hematoxylin? In-Reply-To: References: Message-ID: <5476245379016B4D8212E8BCD11ECFFB15BB1A170D@AMWPVEX01.kci.com> Hi Meryl, What stain are you using your Hematoxylin for? We use Harris Hematoxylin for H&E staining and we filter it each day that we need to use it. We change it once per month. We are also not a high volume lab. We are a histology department in a biotech company. Your suspicions are right that it could be getting diluted by water dripping off the slides if they are not properly drained after the water rinsing step. Sheryl. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meryl Roberts Sent: Sunday, May 06, 2012 4:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how often do you change your hematoxylin? We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Mon May 7 10:16:54 2012 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Mon May 7 10:17:10 2012 Subject: [Histonet] how often do you change your hematoxylin? In-Reply-To: References: Message-ID: We are a small lab and used Vintage for a while and loved the stain. I did notice that both the Eosin and Hemat did become watered down faster than any other brand we had tried and evaporated more. I also could only use it for about 2 wks before having to toss and refill. If it was more cost efficient we would still be using it. We went back to our Gill III and I filter it 2-3 times a week and can use it for about 3 weeks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meryl Roberts Sent: Sunday, May 06, 2012 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how often do you change your hematoxylin? We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Mon May 7 10:45:42 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon May 7 10:45:46 2012 Subject: [Histonet] Neutrophil Antibodies In-Reply-To: <5A605CE38EECB64B94485C02125A0C44300E14389F@LN-MAIL07.ln.burnham.org> References: <5A605CE38EECB64B94485C02125A0C44300E14389F@LN-MAIL07.ln.burnham.org> Message-ID: Hi John, In the past, I used Neutrophil Elastase from Dako on monkey FFPE tissue; no pretreatment needed; dilution with lot used = 1:50. Worked great. Jan Shivers UMN Vet Diag Lab On Sat, May 5, 2012 at 9:59 AM, John Shelley wrote: > Hi All in Histoland, > > Problem solving never ends! I am working up two antibodies for neutrophils > which are NIMP-R14 from Abcam which it is a rat monoclonal and also > Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I > have tried different conditions without any luck and was wondering if > anyone has worked with these antibodies either from Abcam or another > supplier and might have working conditions that have given results in the > past. We are trying this on FFPE sections which the antibodies say they > work on but I am thinking that I may try frozens. I was trying to avoid > this however because I am using these antibodies on mouse WAT. Any help > would be appreciated. Thanks!!! > > Happy Monday morning!!! > Kind Regards! > John J Shelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Mon May 7 12:41:51 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon May 7 12:41:55 2012 Subject: [Histonet] Re: how often do you change your hematoxylin? Message-ID: Meryl Roberts describes a vintage hematoxylin. I didn't know that hematoxylin came in vintages. But here's a poem by Oliver Wendell Holmes Sr., written about 1880, that makes the comparison. http://www.flickr.com/photos/bobrichmond/3866070073/in/set-72157622048353383 Holmes, a nearly forgotten American poet (he wrote "The Chambered Nautilus" and "The Wonderful One Hoss Shay") was a physician whose day job professor of anatomy at Harvard. There was some interest in lead-mordanted hematoxylins at that time, and he may have been working with them. Holmes actually published the poem as you see it on my Flickr link. What would now be called a concrete poem. Bob Richmond Samurai Pathologist Knoxville TN From Maxim_71 <@t> mail.ru Mon May 7 13:52:47 2012 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon May 7 12:51:17 2012 Subject: [Histonet] how often do you change your hematoxylin? Message-ID: <1017378582.20120507215247@mail.ru> Meryl, We uses for more than 15 years a home made regressive hematoxylin Lillie. Our lab stains approximately 5000-6500 slides monthly. We does our stains maually. We never change our solution, but time to time add new hematoxylin for necessary level in stainig dish. Everyday we filter our working solution after workday. Never any metallic film on surface. Before hematoxyline we rinse rack with slides in DW 1 minute and drain excess water. Always very good results. Sincerely, Maxim Peshkov Russia, Taganrog. You wrote: We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl mailto:Maxim_71@mail.ru From Ken_Marissael <@t> vwr.com Mon May 7 13:01:58 2012 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Mon May 7 13:02:03 2012 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 05/07/2012 and will not return until 05/11/2012. I will be away beginning today at our healthcare meeting and will return on Friday 5/11/12. My access to both e-mail and phone will be limited, so you may not receive an immediate response. Should you have an emergency, please contact customer service at 800-932-5000. Press 6 for Healthcare. From cpyse <@t> x-celllab.com Mon May 7 13:07:11 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon May 7 13:07:44 2012 Subject: [Histonet] vaginitis panel In-Reply-To: References: <000601cd2968$de6522c0$9b2f6840$@com> Message-ID: <002601cd2c7c$3a01dd60$ae059820$@com> I know. I was hoping to see what everyone else is doing. -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Friday, May 04, 2012 6:30 PM To: Cynthia Pyse Cc: Subject: Re: [Histonet] vaginitis panel It's Friday :) Noticed none touched this? Sent from my iPhone On May 3, 2012, at 4:11 PM, "Cynthia Pyse" wrote: > Histonetters > > For those of you performing vaginitis panels, how are you billing > these? Do you bill for each separate test or use the combine CPT code? > We are having a discussion on what is our best method of billing. > Thanks in advance for the info. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue May 8 10:25:51 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue May 8 10:29:43 2012 Subject: [Histonet] stainer question Message-ID: Good morning Histoland! It is a sad day here because I am loosing my Leica stainer. It was given to me by another lab and now they want it back. So it is back to hand staining for us. I'm considering purchasing a replacement and I was looking at refurbed stainers. Don't have much room (as usual) and the ST5020 actually took up so much space that we had it in the processing/embedding room which was a little inconvenient. Thinking of one of the more linear types of stainers. They also seem cheaper. Does anybody have one to recommend? Andi Grantham U of A From Ashley.Troutman <@t> Vanderbilt.Edu Tue May 8 12:14:54 2012 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue May 8 12:15:07 2012 Subject: [Histonet] stainer question Message-ID: <7B310892042DA74CB3590053F424CFE6145CB28989@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Andi, I have had good luck with the Shandon Gemini. Small footprint, too. Ashley Troutman BS, HT(ASCP) QIHC Message: 5 Date: Tue, 8 May 2012 08:25:51 -0700 From: "Grantham, Andrea L - (algranth)" > Subject: [Histonet] stainer question To: HISTONET > Message-ID: > Content-Type: text/plain; charset="us-ascii" Good morning Histoland! It is a sad day here because I am loosing my Leica stainer. It was given to me by another lab and now they want it back. So it is back to hand staining for us. I'm considering purchasing a replacement and I was looking at refurbed stainers. Don't have much room (as usual) and the ST5020 actually took up so much space that we had it in the processing/embedding room which was a little inconvenient. Thinking of one of the more linear types of stainers. They also seem cheaper. Does anybody have one to recommend? Andi Grantham U of A From sauconym <@t> yahoo.com Tue May 8 12:58:11 2012 From: sauconym <@t> yahoo.com (Miha Tesar) Date: Tue May 8 12:58:16 2012 Subject: [Histonet] (no subject) Message-ID: <1336499891.91210.YahooMailNeo@web45012.mail.sp1.yahoo.com> Hi! Please erase me from your mailing list! Thank you best regards Miha From SohrabB1 <@t> ah.org Tue May 8 13:57:05 2012 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue May 8 13:57:13 2012 Subject: [Histonet] Cassett Labeler Message-ID: <4FA90A11.4347.0054.1@ah.org> We have to purchase a cassette labeler ASAP, Any suggestions?? Appreciate any help? Behnaz Sohrab From SStephenson <@t> lifecell.com Tue May 8 14:24:25 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Tue May 8 14:24:28 2012 Subject: [Histonet] Cassett Labeler In-Reply-To: <4FA90A11.4347.0054.1@ah.org> References: <4FA90A11.4347.0054.1@ah.org> Message-ID: <5476245379016B4D8212E8BCD11ECFFB15BB1A1712@AMWPVEX01.kci.com> We use PrintMate by Thermo Fisher. Kinda loud by its pretty neat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, May 08, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassett Labeler We have to purchase a cassette labeler ASAP, Any suggestions?? Appreciate any help? Behnaz Sohrab From mucram11 <@t> comcast.net Tue May 8 14:39:53 2012 From: mucram11 <@t> comcast.net (mucram11@comcast.net) Date: Tue May 8 14:40:04 2012 Subject: [Histonet] Cassett Labeler In-Reply-To: <5476245379016B4D8212E8BCD11ECFFB15BB1A1712@AMWPVEX01.kci.com> References: <4FA90A11.4347.0054.1@ah.org> <5476245379016B4D8212E8BCD11ECFFB15BB1A1712@AMWPVEX01.kci.com> Message-ID: <636109955-1336505994-cardhu_decombobulator_blackberry.rim.net-1684895385-@b13.c13.bise6.blackberry> We agree. Had them for a year and half and love the cassette writer and slide writers. Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Stephenson, Sheryl" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Tue, 8 May 2012 14:24:25 To: 'Behnaz Sohrab'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassett Labeler We use PrintMate by Thermo Fisher. Kinda loud by its pretty neat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, May 08, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassett Labeler We have to purchase a cassette labeler ASAP, Any suggestions?? Appreciate any help? Behnaz Sohrab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mdpraet <@t> gmail.com Tue May 8 16:17:21 2012 From: mdpraet <@t> gmail.com (mequita praet) Date: Tue May 8 16:17:24 2012 Subject: [Histonet] Stainers Message-ID: Hey Andi, If you can get a Leica XL they are terrific. Real workhorses. Might cost a little more but well worth it. You can even do some special staining on it, at least part of the special. You should be able to get a refurbished one. If you need some contacts with the companies that sell refurbished equipment let me know. Mequita Praet Histology Pro Consulting, LLC From nicole <@t> dlcjax.com Wed May 9 08:49:12 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed May 9 08:49:22 2012 Subject: [Histonet] Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs Message-ID: <4637.208.62.167.196.1336571352.squirrel@webmail.realpages.com> ---------------------------- Original Message ---------------------------- Subject: Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs From: blondi33331@aol.com Date: Tue, May 8, 2012 3:57 pm To: nicole@dlcjax.com -------------------------------------------------------------------------- -----Original Message----- From: nfe1244 To: seaglstein ; pam ; blondi33331 Sent: Sun, May 6, 2012 9:06 am Subject: Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs good news! -----Original Message----- From: American Academy of Dermatology Association To: N. Fred Eaglstein; DO; FAAD Sent: Fri, May 4, 2012 5:58 pm Subject: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs If you can't see the images in this email, please click here. This week???s headlines: Indoor tanning bed labeling legislation introduced, fails to include FDA reclassification House and Senate Committees continue working on prescription drug, medical device legislation AADA provides comments to the FDA regarding biosimilars AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs Dermatology societies to collaboratively share recently approved AUC for Mohs surgery with payers Vermont becomes second state in the nation to ban tanning for minors California patient safety bill moves swiftly through state assembly Cosmetic tax proposals stripped from California bills Tanning bill endorsed by Missouri House of Representatives Mississippi enacts ??Patient??s Right to Informed Health Care Choices Act?? Maryland enacts board certification disclosure requirements SkinPAC to host fundraiser in Chicago Register now for the 2012 AADA Legislative Conference, Sept. 9 ?? 11, Washington D.C. Congressional action Indoor tanning bed labeling legislation introduced, fails to include FDA reclassification On April 19, Sens. Jack Reed (D-R.I.) and Johnny Isakson (R-Ga.) introduced S. 2301, the Tanning Transparency and Notification Act of 2012 which calls on the FDA to enhance indoor tanning bed labeling requirements based on recommendations the agency made as a result of the TAN Act of 2006. The AADA has been working closely with Sen. Reed??s office to introduce a Senate companion bill to H.R. 1676, the Tanning Bed Cancer Control Act, which calls on the Food and Drug Administration (FDA) to reclassify indoor tanning beds, however Sen. Reed introduced his new Senate legislation removing the AADA-supported FDA reclassification language and leaving only the enhanced labeling portion of the bill. The AADA sent a letter to both senators stating the importance of including language calling on the FDA to reclassify tanning beds, in addition to the enhanced labeling requirements that, alone, do little to deter the use of indoor tanning beds. House and Senate committees continue working on prescription drug, medical device legislation The House Energy & Commerce Committee and the Senate Health, Education, Labor, and Pensions (HELP) Committee are working on a bipartisan effort to reauthorize prescription drug and medical device user fee legislation. The resulting bills are expected to come to the House and Senate floors for action sometime in June. Both the House and Senate draft bills include language to address the ongoing prescription drug shortages that physicians across the country are facing and propose solutions to mitigate future shortages. Additionally, the Senate HELP Committee version currently includes legislation (S. 2301) introduced by Sens. Jack Reed (D-R.I.) and Johnny Isakson (R-Ga.) calling on the FDA to enhance indoor tanning bed labeling requirements based on the agency??s recommendations (see story above). As the Committee process moves forward, the AADA is monitoring the legislation and urging Congress to also include language calling on the FDA to reclassify indoor tanning beds. Federal agency focus AADA provides comments to the FDA regarding biosimilars On May 11, the Food and Drug Administration will convene a public hearing regarding biosimilars. The hearing will include conversations on naming, labeling, and pharmacovigilance of these new therapies. In anticipation of the hearing, the AADA has submitted comments to the agency highlighting our continued concerns regarding naming and pharmacoviligance issues. The Academy urges the agency to provide unique non-proprietary names for all biosimilars to reduce any confusion with the reference biologic products. An update on the May 11 meeting will appear in the next issue of Dermatology Advocate. Private payer activity AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs Aetna has assured the Academy that dermatology practices with in-office laboratories will be exempted from a recently announced policy requiring practices to be both Clinical Laboratory Improvement Amendments (CLIA) certified and College of American Pathologists (CAP) accredited to receive payment for surgical pathology services. The Academy took the lead on this issue and has been working closely with Aetna since early April to address concerns about the recent change. As a result, Aetna has agreed that dermatologists do not need to provide additional accreditation documentation. Aetna expects to provide written notification to affected practices in the next few weeks. Dermatology societies to collaboratively share recently approved AUC for Mohs surgery with payers The Academy is collaborating with the American College of Mohs Surgery, American Society for Dermatologic Surgery Association, and American Society for Mohs Surgery to engage in an ongoing dialogue with payers about the recently approved Appropriate Use Criteria for Mohs surgery (Mohs AUC). Next week, the societies will begin this dialogue by sending a joint letter to Novitas Solutions, Inc. (aka Highmark Medicare Services, Inc.) which will provide context for interpreting the Mohs AUC, and communicate how the Mohs AUC support the clinical indications for Mohs surgery, which will serve to preserve its use for our patients. This coordinated effort will help minimize misinterpretation or misuse of the AUC. The Mohs AUC have been submitted for publication to the Journal of the American Academy of Dermatology and Dermatologic Surgery and are posted to the Academy??s website. Members of the Academy and collaborating organizations are encouraged to review the Mohs AUC and complete the online survey to provide feedback on what educational resources would be helpful to them in implementing the Mohs AUC in their practices, and to provide comments on areas for consideration in future updates of the Mohs AUC. State policy roundup Vermont becomes second state in the nation to ban tanning for minors On May 2, Vermont became the second state in the nation to prohibit the use of tanning devices by children and teens under the age of 18. The AADA and Vermont Dermatological Society (VDS) commended the state legislature for taking the lead on this important public health issue. Vermont was one of nearly 25 states considering legislation related to regulation of the indoor tanning industry and minors?? access this session, and the second state after Utah to enact a bill this year. The AADA and VDS hope Vermont??s leadership will serve as a catalyst for other states with pending legislation that could take action before the end of their 2012 legislative sessions, including Massachusetts, Rhode Island, New York, New Jersey, Ohio, North Carolina, and Pennsylvania. California patient safety bill moves swiftly through state assembly On April 26, the California State Assembly unanimously passed Assembly Bill 1548, cosponsored by the American Society for Dermatologic Surgery Association (ASDSA) and the California Society of Dermatology and Dermatologic Surgery (CalDerm), by a vote of 71 to zero. The bill, which the AADA supported via written comments, will now move on to the California State Senate for further consideration. AB 1548 would increase financial penalties for corporate practice of medicine. The bill, as previously reported here, was passed by the full state legislature on two previous occasions and vetoed by then-Governor Arnold Schwarzenegger. This year??s legislation has been moving swiftly through the legislative process. AADA will continue to support ASDSA and CalDerm in this effort. Cosmetic tax proposals stripped from California bills Last week, two pieces of legislation were amended to remove language that would have applied California state sales tax to cosmetic medical procedures. The AADA and the American Society for Dermatologic Surgery Association jointly commented in opposition to AB 2540 and AB 1963 prior to hearings in Assembly policy committees last month. The AADA will remain vigilant with the ASDSA and the California Society of Dermatology and Dermatologic Surgery to ensure these proposals do not resurface this year. Tanning bill passes Missouri House of Representatives On April 30, the Missouri House of Representatives passed House Bill 1475 by a vote of 91 to 56. The legislation would require minors under the age of 17 to obtain parental consent before using a tanning bed. The bill is now being sent to the state senate for consideration. The Missouri Dermatological Society Association (MDSA) has been working tirelessly for several years, with the support of the AADA, to pass legislation to better regulate the tanning industry in the state, including provisions related to minors?? access. HB 1475 is the first stand-alone bill in two years to proceed to this point in the legislative process. AADA will continue to support the efforts of the MSDA on this issue. Mississippi enacts ??Patient??s Right to Informed Health Care Choices Act?? On April 18, Mississippi Gov. Haley Barbour signed Senate Bill 2670, "The Patient's Right to Informed Health Care Choices Act." The AADA and the Mississippi Dermatological Society supported SB 2670 and its companion bill, House Bill 899, early in the legislative process with written comments. The legislation is based on the American Medical Association??s model bill on truth in advertising, developed by the Scope of Practice Partnership, and helps to ensure that patients are promptly and clearly informed of the actual training and qualifications of their health care practitioners. The bill requires in part that all advertisements for health care services are free from deceptive and misleading information and identify the type of license held by the provider(s). In addition, health care practitioners are required to post in their office and affirmatively communicate their specific license to all patients. The new law is effective July 1. Maryland enacts board certification disclosure requirements At the close of the Maryland legislative session last month, the General Assembly unanimously passed Senate Bill 395 and House Bill 957, requiring all health occupation boards in the state to submit information to the legislature by the end of 2012 on exactly what regulations or policies currently exist for their licensees related to advertising. In addition, the legislation requires any physician who advertises board certification to disclose his or her specialty or subspecialty field of certification and the name of the certifying board. Only physicians with certification from approved boards will be permitted to advertise board certification. Gov. O??Malley signed both pieces of legislation on May 2, and the new law will take effect on Oct. 1. Political affairs ?? SkinPAC SkinPAC to host fundraiser in Chicago SkinPAC will be having a fundraiser in Chicago on Saturday, May 5, 2012 from 7 to 9 p.m. in a private suite, room 3712, at the Fairmont Hotel. This reception will coincide with the American College of Mohs Surgery Annual Meeting, but is not associated with the organization; however Mohs surgeons have been invited to attend. The event is being hosted by Brett Coldiron, MD, Brent Moody, MD, and Pat Davey, MD. The host committee is asking for a $500 contribution to attend the event. Cocktails and hors d??oeuvres will be served. If you would like to attend, please RSVP to Sam Hewitt, the AADA??s manager of political affairs, at (202) 712-2609, or by email at shewitt@aad.org. SkinPAC??s political purpose is to solicit and receive contributions to be used to make political campaign expenditures to those candidates for federal elective office, and other federal political committees, who demonstrate understanding and interest in the views and goals of the American Academy of Dermatology Association. Contributions to SkinPAC are not deductible as charitable contributions for federal income tax purposes. SkinPAC cannot accept contributions from corporate accounts. All AADA members have the right to refuse to contribute without reprisal. Federal law prohibits us from accepting contributions from foreign nationals. Federal law requires us to use our best efforts to collect and report the name, physical address, occupation, and the name of the employer of individuals whose contributions exceed $200 in a calendar year. Upcoming events Register now for the 2012 AADA Legislative Conference, Sept 9 ?? 11, Washington, D.C. The AADA is pleased to invite the entire Academy membership to register for the 2012 Legislative Conference, Sept. 9 ?? 11 at the Crystal Gateway Marriott, 10 minutes from the US Capitol. The Legislative Conference is a unique blend of didactic and interactive advocacy training sessions, political debate and the direct lobbying of your members of Congress. Conference attendees learn about critical health policy issues from national health policy experts, political insiders, and AADA advocacy leaders. A keynote address will be delivered by former CMS administrator, Mark McClellan, MD, PhD. However, the most powerful aspect of the Legislative Conference is your ability to build and strengthen personal relationships with your members of Congress and their staff. If you no longer wish to receive future emails from the Academy, please click here. -------------- next part -------------- -----Original Message----- From: American Academy of Dermatology Association <[1]noreply@aadassociation.org> To: N. Fred Eaglstein; DO; FAAD <[2]nf Sent: Fri, May 4, 2012 5:58 pm Subject: AADA rapid response team convinces Aetna to clarify policy on ac If you can't see the images in this email, please < #cccccc; TEXT-DECORATION: underline; text-underline: si href="http://lyris.neton-line.com/t/46093/2710654/1531/0/" target =_blank>click here. [3][test.neton-line.c=] This week??? * [4]Indoor tanning bed labeling legislation introduced, fa include FDA reclassification * [5]House and Senate Committees continue working on prescr drug, medical device legislation * [6]AADA provides comments to the FDA regarding biosimilar * [7]AADA rapid response team convinces Aetna to clarify po accreditation for in-office pathology labs * [8]Dermatology societies to collaboratively share recentl approved AUC for Mohs surgery with payers * [9]Vermont becomes second state in the nation to ban tann minors * [10]California patient safety bill moves swiftly through s assembly * [11]Cosmetic tax proposals stripped from California bills< * [12]Tanning bill endorsed by Missouri House of Representat * [13]Mississippi enacts ??Patient??s Right to Info Health Care Choices Act?? * [14]Maryland enacts board certification disclosure requir * [15]SkinPAC to host fundraiser in Chicago * [16]Register now for the 2012 AADA Legislative Conference 9 ?? 11, Washington D.C. Congressional action Indoor tanni FDA reclassifica On April 19, Sens. Jack Reed (D-R.I.) and Johnny Isakson (R-Ga.) introduc Act of 2 labeling require result of the TAN Act Sen. Reed??s office 1676, the Tanning Bed Cancer Drug Administration (FDA) to re Sen. Reed introduced his new Senate AADA-supported FDA reclassification language an enhanced labeling portion of the bill. The AADA sent a both senators stating the importance of including la on the FDA to reclassify tanning beds , in addition to the enhanced labeling requirements that, alone, do litt indoor tanning beds. House and Senate committees continue working on prescription drug, m edical device legislation The House Energy & Commerce Committee and the Senate Health, Educatio n, Labor, and Pensions (HELP) Committee are working on a bipartisan effor legislatio and Senate floor Senate draft bills incl prescription drug shortages that phys facing and propose solutions to mitigate fu Additionally, the Senate HELP Committee version currently [19]legislation (S. 2301) introduced by Sens. Jack Reed Johnny Isakson (R-Ga.) calling on the FDA to enhance indoor bed labeling requirements based on the agency??s recommendati (see story above). As the Committee process moves forward, the AADA i s monitoring the legislation and urging Congress to also include language calling on the FDA to reclassify indoor tanning Federal agency focus AADA provides comments to the FDA regarding biosimilars On May 11, the Food and Drug Administration will convene a public hearing on naming, anticipation the agency pharmacoviliganc unique non-proprietary confusion with the reference biol 11 meeting will appear in the next is Private payer activity accreditation for in-office pathology labs Aetna has assured the Academy that dermatology practices with in-office l policy requiring p Improvement Amendments (CLIA) cer Pathologists (CAP) accredited to receive p pathology services. The Academy took the lead on this been working closely with Aetna since early April to addre concerns about the recent change. As a result, Aetna has agreed that d documentati affected practices i Dermatology for Mohs surgery The Academy is collaborating with the American College of Mohs Surgery, A American Societ with payers about the Mohs surgery (Mohs AUC). dialogue by sending a joint lett Highmark Medicare Services, Inc.) whic interpreting the Mohs AUC, and communicate how the clinical indications for Mohs surgery, which wi preserve its use for our patients. This coordinated effort wi minimize misinterpretation or misuse of the AUC. The Mohs AUC have been submitted for publ the American Academy of Dermatology an posted to the [21]Academy?? collaborating organizations are complete the online survey to prov resources would be helpful to them in im their practices, and to provide comments on ar in future updates of the Mohs AUC. State policy roundup Vermont becomes second state in the nation to ban tanning for minors On May 2, Vermont became the second state in the nation to prohibit the u 18. The AADA the state health issue. Ve legislation related to regu minors?? access this sessio enact a bill this year. The AADA an leadership will serve as a catalyst for other s legislation that could take action before the end of t legislative sessions, including Massachusetts, Rhode Island, Ne York, New Jersey, Ohio, North Carolina, and Pennsylvania. California patient safety bill moves swiftly through state assembly< /STRONG> On April 26, the California State Assembly unanimously passed Assembly Bi Surgery Ass Dermatology and Dermatolog zero. The bill, which the AADA s now move on to the California State S consideration. AB 1548 would increase financial penalti corporate practice of medicine. The bill, as previously reported h ere, was passed by the full state legislature on two previous occasions a year??s legisl legislative process. AADA will in this effort. Cosmetic tax proposals stripped from California bills Last week, two pieces of legislation were amended to remove language that medical proced Dermatologic Surgery Associat AB 2540 and AB 1963 prior to heari last month. The AADA will remain vigila California Society of Dermatology and Dermatolo these proposals do not resurface this year. Tanning bill passes Missouri House of Representatives On April 30, the Missouri House of Representatives passed House Bill 1475 under the ag tanning bed. The bill i consideration. The Missouri Derm (MDSA) has been working tirelessly for sev support of the AADA, to pass legislation to better r tanning industry in the state, including provisions related t minors?? access. HB 1475 is the first stand-alone bill in two years continue Mississippi enacts ??Patient??s Right to Informed Health Ca re Choices Act?? On April 18, Mississippi Gov. Haley Barbour signed Senate Bill 2670, "The and the M companion bil with written comments Medical Association??s mod developed by the Scope of Practice Partn that patients are promptly and clearly inform training and qualifications of their health care practit bill requires in part that all advertisements for health care services are free from deceptive and misleading information and identify health care pr affirmatively commun The new law is effective Ju Maryland enacts board certification disclosure requirements At the close of the Maryland legislative session last month, the General 957, requiring information to the l regulations or policies cur to advertising. In addition, the who advertises board certification to or subspecialty field of certification and board. Only physicians with certification fro be permitted to advertise board certification. Gov signed both pieces of legislation on May 2, and the new l take effect on Oct. 1. Political affairs ?? SkinPA SkinPAC to host fundraiser in Chicago SkinPAC will be having a fundraiser in Chicago on Saturday, May 5, 2012 f [23]Fairmont Hot College of Mohs Su the organization; howeve The event is being hosted by Pat Davey, MD. The host committ to attend the event. Cocktails and h served. If you would like to attend, please RS AADA??s manager of political affairs, at (202) email at [24]shewitt@aad.o SkinPAC??s polit contributions to be used to make p those candidates for federal elective o political committees, who demonstrate understand the views and goals of the American Academy of Dermat Association. Contributions to SkinPAC are not deductible as charitable contributions contributions from right to refuse to contrib prohibits us from accepting contributio Federal law requires us to use our best effort report the name, physical address, occupation, and the n employer of individuals whose contributions exceed $200 in a c alendar year. Upcoming events Register now for the 2012 AADA Legislative Con 11, Washington, D.C. The AADA is pleased to invite the entire Academy membership to [25]reg The L interactive advoc direct lobbying of your m learn about critical health poli policy experts, political insiders, and AA keynote address will be delivered by former CMS ad McClellan, MD, PhD. However, the most powerful aspect o Legislative Conference is your ability to build and strengthen pers onal relationships with your members of Congress and their staff. 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(HT)ASCP, QIHC Please do not respond in the histo-net, but to my private e-mail bill@hphisto.com Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar Sent: Tuesday, May 08, 2012 12:58 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! Please erase me from your mailing list! Thank you best regards Miha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From kshimp <@t> seattlecca.org Wed May 9 12:37:01 2012 From: kshimp <@t> seattlecca.org (Shimp, Kristen R) Date: Wed May 9 12:37:07 2012 Subject: [Histonet] WSHS Spring Meeting Message-ID: <24041_1336585023_4FAAAB3F_24041_51_1_B4C7785E2A17AE4D932107D2EFA36AD44A6F20D7@EXDB01.seattlecca.org> The Washington State Histology Society is hosting a Spring continuing education meeting on May 19, 2012 in Everett, Washington. Early registration is still available. We have a variety of class topics including lung cancer research, double IHC staining techniques, and microbiology. A copy of the program and registration form can be found at www.wahisto.wordpress.com. This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 624-1159, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From kaitlin <@t> prometheushealthcare.com Wed May 9 14:27:13 2012 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Wed May 9 14:27:18 2012 Subject: [Histonet] Manager needed for NC Message-ID: <018a01cd2e19$bcd8fe50$368afaf0$@prometheushealthcare.com> My team and I are currently seeking a qualified Histology Manager for one of our top facilities in North Carolina. The ideal candidate will have a minimum of 3-5 years of management experience, HT/HTL ASCP certification and a BA/BS. The facility processes mostly IHC and surgical specimens. The position offers a great location to live, highly competitive salary with annual bonus, relocation package and full benefits. Please contact me to inquire about the opening. Kaitlin Webster Recruiting Manager Prometheus Healthcare Kaitlin@prometheushealthcare.com From Vickroy.Jim <@t> mhsil.com Thu May 10 07:37:09 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu May 10 07:37:14 2012 Subject: [Histonet] Grossing Cassette Baskets Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C4721AB66@mmc-mail.ad.mhsil.com> Does anybody know where we can purchase wire or plastic tissue cassette baskets for our grossing stations. We are looking for baskets that will keep the cassettes in line similar to the old VIP 3000 metal baskets with dividers. In our workflow we later transfer the cassettes to processing baskets. (Looked into purchasing more processing baskets, but at 400-500 a basket that doesn't seem to feasible.) Surely someone has old wire baskets or new plastic ones. Thanks for your help. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From GaleL <@t> unionhospital.org Thu May 10 08:26:47 2012 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Thu May 10 08:26:54 2012 Subject: [Histonet] formalin substitute Message-ID: Is there any problem (to tissue or processor) adding tissue fixed in a formalin substitute to a processor where 10% NBF is used? Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From ftryka <@t> tetonhospital.org Thu May 10 09:42:43 2012 From: ftryka <@t> tetonhospital.org (Tryka, A. Francine) Date: Thu May 10 09:42:53 2012 Subject: [Histonet] RE: formalin substitute In-Reply-To: References: Message-ID: I supply Excell to a clinician allergic to formalin. These are small GI biopsy specimens and I place them directly into our 10% NBF (thus, no special handling) and see no difference in the histology, nor is there any problem with sectioning. F Tryka, M.D. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gale Limron [GaleL@unionhospital.org] Sent: Thursday, May 10, 2012 7:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin substitute Is there any problem (to tissue or processor) adding tissue fixed in a formalin substitute to a processor where 10% NBF is used? Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. From Donna.Willis <@t> baylorhealth.edu Thu May 10 10:59:21 2012 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu May 10 10:59:32 2012 Subject: [Histonet] Productivity Standards Message-ID: <3FA597486B013249A2FC8EE113CBCC02196A4EC1BD@BHDAEXVM33.bhcs.pvt> Is anyone out there in Histoland willing to share their minimum productivity standards for embedding and microtomy. Quality is just as important as quantity. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From aonomic <@t> auburn.edu Thu May 10 11:02:07 2012 From: aonomic <@t> auburn.edu (Michelle Aono) Date: Thu May 10 11:02:16 2012 Subject: [Histonet] Brown Adipose Tissue Message-ID: <3A862E73E3BAEF4ABCA665F7E3AF064012DFC1C6@EXMB2.auburn.edu> Hi Histonetters! Does anyone have a protocol for fixing, processing, sectioning brown adipose? I don't have any experience with adipose so a detailed description would be fantastic. I think the tissue will be coming from squirrels. Thanks! From kblack <@t> digestivehlth.com Thu May 10 13:32:45 2012 From: kblack <@t> digestivehlth.com (Konni Black) Date: Thu May 10 13:32:50 2012 Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 11 In-Reply-To: <201205101703.q4AH3ZBf008028@hlth24.securesites.net> References: <201205101703.q4AH3ZBf008028@hlth24.securesites.net> Message-ID: Pretty cool. Fun accessories at really good prices. Don't know if I would buy much but I loved 2 pair of earrings on there that were soldout! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 10, 2012 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 102, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. WSHS Spring Meeting (Shimp, Kristen R) 2. Manager needed for NC (Kaitlin Webster) 3. Grossing Cassette Baskets (Vickroy, Jim) 4. formalin substitute (Gale Limron) 5. RE: formalin substitute (Tryka, A. Francine) 6. Productivity Standards (Willis, Donna G.) 7. Brown Adipose Tissue (Michelle Aono) ---------------------------------------------------------------------- Message: 1 Date: Wed, 9 May 2012 17:37:01 +0000 From: "Shimp, Kristen R" Subject: [Histonet] WSHS Spring Meeting To: "histonet@lists.utsouthwestern.edu" Message-ID: <24041_1336585023_4FAAAB3F_24041_51_1_B4C7785E2A17AE4D932107D2EFA36AD44A6F20D7@EXDB01.seattlecca.org> Content-Type: text/plain; charset="us-ascii" The Washington State Histology Society is hosting a Spring continuing education meeting on May 19, 2012 in Everett, Washington. Early registration is still available. We have a variety of class topics including lung cancer research, double IHC staining techniques, and microbiology. A copy of the program and registration form can be found at www.wahisto.wordpress.com. This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 624-1159, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ------------------------------ Message: 2 Date: Wed, 9 May 2012 15:27:13 -0400 From: "Kaitlin Webster" Subject: [Histonet] Manager needed for NC To: Message-ID: <018a01cd2e19$bcd8fe50$368afaf0$@prometheushealthcare.com> Content-Type: text/plain; charset="us-ascii" My team and I are currently seeking a qualified Histology Manager for one of our top facilities in North Carolina. The ideal candidate will have a minimum of 3-5 years of management experience, HT/HTL ASCP certification and a BA/BS. The facility processes mostly IHC and surgical specimens. The position offers a great location to live, highly competitive salary with annual bonus, relocation package and full benefits. Please contact me to inquire about the opening. Kaitlin Webster Recruiting Manager Prometheus Healthcare Kaitlin@prometheushealthcare.com ------------------------------ Message: 3 Date: Thu, 10 May 2012 07:37:09 -0500 From: "Vickroy, Jim" Subject: [Histonet] Grossing Cassette Baskets To: "histonet@lists.utsouthwestern.edu" Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C4721AB66@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" Does anybody know where we can purchase wire or plastic tissue cassette baskets for our grossing stations. We are looking for baskets that will keep the cassettes in line similar to the old VIP 3000 metal baskets with dividers. In our workflow we later transfer the cassettes to processing baskets. (Looked into purchasing more processing baskets, but at 400-500 a basket that doesn't seem to feasible.) Surely someone has old wire baskets or new plastic ones. Thanks for your help. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 4 Date: Thu, 10 May 2012 09:26:47 -0400 From: Gale Limron Subject: [Histonet] formalin substitute To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Is there any problem (to tissue or processor) adding tissue fixed in a formalin substitute to a processor where 10% NBF is used? Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. ------------------------------ Message: 5 Date: Thu, 10 May 2012 14:42:43 +0000 From: "Tryka, A. Francine" Subject: [Histonet] RE: formalin substitute To: Gale Limron , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I supply Excell to a clinician allergic to formalin. These are small GI biopsy specimens and I place them directly into our 10% NBF (thus, no special handling) and see no difference in the histology, nor is there any problem with sectioning. F Tryka, M.D. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gale Limron [GaleL@unionhospital.org] Sent: Thursday, May 10, 2012 7:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin substitute Is there any problem (to tissue or processor) adding tissue fixed in a formalin substitute to a processor where 10% NBF is used? Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. ------------------------------ Message: 6 Date: Thu, 10 May 2012 10:59:21 -0500 From: "Willis, Donna G." Subject: [Histonet] Productivity Standards To: "histonet@lists.utsouthwestern.edu" Message-ID: <3FA597486B013249A2FC8EE113CBCC02196A4EC1BD@BHDAEXVM33.bhcs.pvt> Content-Type: text/plain; charset="ISO-8859-1" Is anyone out there in Histoland willing to share their minimum productivity standards for embedding and microtomy. Quality is just as important as quantity. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 7 Date: Thu, 10 May 2012 16:02:07 +0000 From: Michelle Aono Subject: [Histonet] Brown Adipose Tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: <3A862E73E3BAEF4ABCA665F7E3AF064012DFC1C6@EXMB2.auburn.edu> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! Does anyone have a protocol for fixing, processing, sectioning brown adipose? I don't have any experience with adipose so a detailed description would be fantastic. I think the tissue will be coming from squirrels. Thanks! ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 102, Issue 11 ***************************************** From mitchell <@t> wanpost.com Thu May 10 17:57:15 2012 From: mitchell <@t> wanpost.com (Mitchell Wan) Date: Thu May 10 17:57:26 2012 Subject: [Histonet] Re. Grossing Cassette Baskets Message-ID: <002101cd2f00$3e7eb850$bb7c28f0$@com> Hi James, I am interested in locating some baskets as well. I am tempted to go to a local metal works shop and ask them to make some stainless steel ones for myself. It will be approx. 1/10 of the price. Regards Mitchell Wan InfinityPATH Pty Ltd M: 0418 745 750 P: 07 3123 8888 F: 07 3123 8889 E: mitchell.wan@infinitypath.com.au W: www.infinitypath.com.au Does anybody know where we can purchase wire or plastic tissue cassette baskets for our grossing stations. We are looking for baskets that will keep the cassettes in line similar to the old VIP 3000 metal baskets with dividers. In our workflow we later transfer the cassettes to processing baskets. (Looked into purchasing more processing baskets, but at 400-500 a basket that doesn't seem to feasible.) Surely someone has old wire baskets or new plastic ones. Thanks for your help. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 From Dorothy.L.Webb <@t> HealthPartners.Com Fri May 11 09:11:08 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri May 11 09:11:30 2012 Subject: [Histonet] cryptosopridia Message-ID: <65365F35C0F2EF4D846EC3CA73E49C430190CE5A09BB@HPEMX3.HealthPartners.int> What stain is anyone using for cryptosporidia in histology?? I thought this was more of a microbiology test. Could use some help please!! Thanks, Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Rcartun <@t> harthosp.org Fri May 11 09:25:15 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri May 11 09:25:26 2012 Subject: [Histonet] cryptosopridia In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C430190CE5A09BB@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C430190CE5A09BB@HPEMX3.HealthPartners.int> Message-ID: <4FACE90B.7400.0077.1@harthosp.org> Yes, most testing is performed in the Microbiology Laboratory; however, I have an IHC test for formalin-fixed, paraffin-embedded tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Webb, Dorothy L" 5/11/2012 10:11 AM >>> What stain is anyone using for cryptosporidia in histology?? I thought this was more of a microbiology test. Could use some help please!! Thanks, Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri May 11 11:21:17 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 11 11:21:15 2012 Subject: [Histonet] Re. Grossing Cassette Baskets In-Reply-To: <002101cd2f00$3e7eb850$bb7c28f0$@com> References: <002101cd2f00$3e7eb850$bb7c28f0$@com> Message-ID: <8D7C2D242DBD45498006B21122072BF8B4D99AF8@MCINFRWEM003.ucsfmedicalcenter.org> You can get the old-style sakura baskets from this company. These are the ones in which the plastic cassettes lay on their sides. They are about half the price. ***************** http://store.techonebiomedical.com/store/ We sell the old style Sakura baskets. We have the 50 and 100 cassette models. We also sell a 150 cassette deluxe model. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 **************************** -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Wan Sent: Thursday, May 10, 2012 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re. Grossing Cassette Baskets Hi James, I am interested in locating some baskets as well. I am tempted to go to a local metal works shop and ask them to make some stainless steel ones for myself. It will be approx. 1/10 of the price. Regards Mitchell Wan InfinityPATH Pty Ltd M: 0418 745 750 P: 07 3123 8888 F: 07 3123 8889 E: mitchell.wan@infinitypath.com.au W: www.infinitypath.com.au Does anybody know where we can purchase wire or plastic tissue cassette baskets for our grossing stations. We are looking for baskets that will keep the cassettes in line similar to the old VIP 3000 metal baskets with dividers. In our workflow we later transfer the cassettes to processing baskets. (Looked into purchasing more processing baskets, but at 400-500 a basket that doesn't seem to feasible.) Surely someone has old wire baskets or new plastic ones. Thanks for your help. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrewcoleman131 <@t> gmail.com Fri May 11 11:25:19 2012 From: andrewcoleman131 <@t> gmail.com (Andrew Coleman) Date: Fri May 11 11:25:29 2012 Subject: [Histonet] Xylene Substitute for Counterstain Clearing Message-ID: Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew From liz <@t> premierlab.com Fri May 11 11:35:47 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri May 11 11:35:55 2012 Subject: [Histonet] Xylene Substitute for Counterstain Clearing In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5D9F@SBS2K8.premierlab.local> Andrew There are aqueous permanent mounting medias such as Advantage Permanent Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years since I used it but it does work on some applications. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Coleman Sent: Friday, May 11, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute for Counterstain Clearing Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SohrabB1 <@t> ah.org Fri May 11 12:29:59 2012 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Fri May 11 12:30:10 2012 Subject: [Histonet] Fwd: Plants References: <4FABE0A2.4347.0054.1@ah.org> Message-ID: <4FACEA27.4347.0054.1@ah.org> I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz From chapcl <@t> yahoo.com Fri May 11 12:32:37 2012 From: chapcl <@t> yahoo.com (William) Date: Fri May 11 12:32:48 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <4FACEA27.4347.0054.1@ah.org> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules. Will Chappell Sent from my iPhone On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote: > > I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? > Thank you, Behnaz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Fri May 11 12:41:33 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri May 11 12:42:20 2012 Subject: [Histonet] Xylene Substitute for Counterstain Clearing In-Reply-To: References: Message-ID: <001e01cd2f9d$4eaeec70$ec0cc550$@com> Andrew You could use Clearium from Leica. Clearium can either be coverslipped from xylene or isopropyl alcohol. Drying time from isopropyl is longer then xylene. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Coleman Sent: Friday, May 11, 2012 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute for Counterstain Clearing Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri May 11 12:58:39 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri May 11 12:58:44 2012 Subject: [Histonet] paraffin melting in VIP Message-ID: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun From bakevictoria <@t> gmail.com Fri May 11 13:17:39 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri May 11 13:17:43 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <4FACEA27.4347.0054.1@ah.org> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: It's probably more toxic for the plants, but I like having them and no one has told me I had to remove them. Ivy's are the most sturdy and the green color just perks up things. On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab wrote: > > I was told by infectious control person that plants are not allowed in the > lab?? IS this true? any experience with this? > Thank you, Behnaz > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From bakevictoria <@t> gmail.com Fri May 11 13:21:45 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri May 11 13:21:49 2012 Subject: [Histonet] Xylene Substitute for Counterstain Clearing In-Reply-To: References: Message-ID: I think that it is called Crystal Mount - but apply to section, allow to harden dip slide in clearing media and coverslip. I know that there must be others out there as well. Good Luck On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman wrote: > Hi all, > > We are performing a neutral red counterstain on tissue sections > containing colored polystyrene microspheres. The spheres are inert to > alcohol, but are washed out when we clear with xylene to coverslip. > The spheres are also supposedly soluble in DMF, acetone, acetonitrile, > chloroform and methylene chloride for what its worth. > > Is it reasonable to coverslip these slides in permanent mount without > clearing with xylene after dehydrating the tissue? Or does anyone know > of a substitute clearing agent with chemical properties dissimilar > enough from xylene that might be worth trying instead? > > Thanks, > > Andrew > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri May 11 13:22:57 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 11 13:23:04 2012 Subject: [Histonet] Xylene Substitute for Counterstain Clearing In-Reply-To: Message-ID: <1336760577.37265.YahooMailClassic@web162101.mail.bf1.yahoo.com> After staining dry the sections in an oven at 60?C for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. Ren? J. --- On Fri, 5/11/12, Andrew Coleman wrote: From: Andrew Coleman Subject: [Histonet] Xylene Substitute for Counterstain Clearing To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 12:25 PM Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 11 13:27:36 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 11 13:27:40 2012 Subject: [Histonet] paraffin melting in VIP In-Reply-To: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> Message-ID: <1336760856.98785.YahooMailClassic@web162102.mail.bf1.yahoo.com> Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!. Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin?at the temperature you desire. Ren? J. --- On Fri, 5/11/12, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] paraffin melting in VIP To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 1:58 PM HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Fri May 11 13:37:21 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Fri May 11 13:37:27 2012 Subject: [Histonet] paraffin melting in VIP In-Reply-To: <1336760856.98785.YahooMailClassic@web162102.mail.bf1.yahoo.com> References: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> <1336760856.98785.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: I agree Rene! I also believe Sakura recommends not putting paraffin flakes directly in the containers. On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa wrote: > Regardless of the time it takes or of how many people do it, melting the > paraffin directly in the VIP should not be done because it causes the > heating elements to work extra reducing their useful life. They are quite > expensive to replace!. > Melt the paraffin outside the VIP and use the VIP only to keep the melted > paraffin at the temperature you desire. > Ren? J. > > --- On Fri, 5/11/12, Gudrun Lang wrote: > > > From: Gudrun Lang > Subject: [Histonet] paraffin melting in VIP > To: histonet@lists.utsouthwestern.edu > Date: Friday, May 11, 2012, 1:58 PM > > > HI! > > A question for those, who melt the paraffin directly in the VIP. How long > does it take to melt the pellets in the VIP-oven? > > > > Thanks > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From sdysart <@t> mirnarx.com Fri May 11 13:51:56 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Fri May 11 13:52:11 2012 Subject: [Histonet] paraffin melting in VIP In-Reply-To: <1336760856.98785.YahooMailClassic@web162102.mail.bf1.yahoo.com> References: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> <1336760856.98785.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50066E2135@BL2PRD0710MB363.namprd07.prod.outlook.com> This is probably a best case scenario, but for labs like the one I am currently in I melt it in the processor. My lab doesn't have the funds to buy me a melting pot (I have had them in all my other labs, just don't know what they are technically called). To answer your question it usually takes overnight and it's melted, but sometimes I end up having to add a little more to get to top off level, that takes a couple hours. Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 11, 2012 1:28 PM To: histonet@lists.utsouthwestern.edu; gu.lang@gmx.at Subject: Re: [Histonet] paraffin melting in VIP Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!. Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin?at the temperature you desire. Ren? J. --- On Fri, 5/11/12, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] paraffin melting in VIP To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 1:58 PM HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri May 11 14:31:00 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri May 11 14:31:05 2012 Subject: AW: [Histonet] paraffin melting in VIP In-Reply-To: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> References: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> Message-ID: <003b01cd2fac$98dca5d0$ca95f170$@gmx.at> Thanks for your kind responses! Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Freitag, 11. Mai 2012 19:59 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] paraffin melting in VIP HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri May 11 14:33:22 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri May 11 14:33:29 2012 Subject: [Histonet] paraffin melting in VIP In-Reply-To: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> References: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> Message-ID: When it is time to exchange reagents, the paraffin is the first thing we do because of the melting time required. We fill up the bin and place it in the oven until there is room to add more wax pellets - we do not stuff it in, but leave it rather loose. We do this about six times during the day until the bin is full. The paraffin pellets are not "cold" and there is a lot of residual heat in the oven due to the three hot wax bins already at temperature, so I am not sure how much "stress" this places on the heating elements. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, May 11, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin melting in VIP HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri May 11 15:07:22 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri May 11 15:07:25 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> Message-ID: <1336766842.44972.YahooMailNeo@web112312.mail.gq1.yahoo.com> Hate to say it, but yes plants are considered infectious. Thats why you cant take them in ICU's either. I guess the mold or bacterias can grow on them. Most places let this slide, but some dont. Good luck! ________________________________ From: William To: Behnaz Sohrab Cc: "" Sent: Friday, May 11, 2012 1:32 PM Subject: Re: [Histonet] Fwd: Plants I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules. Will Chappell Sent from my iPhone On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote: > > I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? > Thank you, Behnaz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lguernsey <@t> ucsd.edu Fri May 11 15:07:11 2012 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Fri May 11 15:07:56 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: I keep a pothos and spider plant in my lab. EH&S has never complained, though I can't say one way or another if it's technically allowed. While my plants are mostly just decorative (I don't think I have enough of them to make much of a difference), it doesn't hurt that they may be filtering our air somewhat. NASA compiled a list of air-filtering plants that can eliminate significant amounts of formaldehyde, xylene, benzene, etc. (Source: http://en.wikipedia.org/wiki/List_of_air-filtering_plants). Lucie UCSD Dept. of Pathology On Friday, May 11, 2012, Victoria Baker wrote: > It's probably more toxic for the plants, but I like having them and no one > has told me I had to remove them. Ivy's are the most sturdy and the green > color just perks up things. > On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab wrote: > > > > > I was told by infectious control person that plants are not allowed in > the > > lab?? IS this true? any experience with this? > > Thank you, Behnaz > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gayle.callis <@t> bresnan.net Fri May 11 16:54:35 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri May 11 16:54:46 2012 Subject: [Histonet] Xylene Substitute for Counterstain Clearing In-Reply-To: <1336760577.37265.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1336760577.37265.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: <000601cd2fc0$a9a2af90$fce80eb0$@bresnan.net> You are also likely to have spheres dissolve in toluene based mounting media. Aqueous media may be the answer unless the neutral red runs out of the tissue in the aqueous environment. Hard to get the best of both worlds if dye runs in aqueous media and spheres dissolve in solvent based . Good luck! Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 11, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu; Andrew Coleman Subject: Re: [Histonet] Xylene Substitute for Counterstain Clearing After staining dry the sections in an oven at 60?C for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. Ren? J. --- On Fri, 5/11/12, Andrew Coleman wrote: From: Andrew Coleman Subject: [Histonet] Xylene Substitute for Counterstain Clearing To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 12:25 PM Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri May 11 16:57:22 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri May 11 16:57:27 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> Message-ID: Yeah but they used to use canaries in mines to warn of toxic levels of gases; having one in a histo lab might be a VERY good idea! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Sent: Friday, May 11, 2012 12:33 PM To: Behnaz Sohrab Cc: Subject: Re: [Histonet] Fwd: Plants I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules. Will Chappell Sent from my iPhone On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote: > > I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? > Thank you, Behnaz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri May 11 16:57:56 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri May 11 16:58:01 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <4FACEA27.4347.0054.1@ah.org> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: I've always had at least one; makes the day more tolerable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, May 11, 2012 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz From talulahgosh <@t> gmail.com Sat May 12 00:12:26 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat May 12 00:12:30 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> Message-ID: A canary? Well, I know what you used that for...how did that work out for you? Actually, I know the answer because you're alive. EH&S should really look into this option, screw the badges... Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Fri, May 11, 2012 at 1:32 PM, William wrote: > I have had plants in a number of labs. Could be against the rules, but I > never saw it. I even had a canary in one lab - pretty sure that is against > the rules. > > Will Chappell > > Sent from my iPhone > > On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote: > > > > > I was told by infectious control person that plants are not allowed in > the lab?? IS this true? any experience with this? > > Thank you, Behnaz > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gayle.callis <@t> bresnan.net Sat May 12 09:48:18 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sat May 12 09:48:29 2012 Subject: [Histonet] Immunofluorescence in the clinical laboratory, some questions Message-ID: <000f01cd304e$467c8380$d3758a80$@bresnan.net> Dear Histonetters, I know that immunofluorescence has been done for decades on renal biopsies, but am curious if laboratories are using IF more these days? If so, I would be very interested to talk to you one on one about this as I have more questions on why you deviate from standard chromogenic enzyme immunohistochemistry and perform IF. Comments about renal biopsy procedures are welcome too. Also, do you do mostly single IF or double IF, and the reasons why? Is your IF done primarily on FFPE or frozen sections/acetone fixation? When you do immunofluorescence in your clinical laboratory are you using an automated stainer or a manual protocol? Or is your clinical laboratory associated with medical research groups? Any comments/information is most welcome. Thanks.............. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From foreightl <@t> gmail.com Sat May 12 10:09:16 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Sat May 12 10:09:21 2012 Subject: [Histonet] Immunofluorescence in the clinical laboratory, some questions In-Reply-To: <000f01cd304e$467c8380$d3758a80$@bresnan.net> References: <000f01cd304e$467c8380$d3758a80$@bresnan.net> Message-ID: Hi Gayle, We perform IF on renal biopsies and dermatological specimens with various bullous disorders. We perform IgA, IgM, IgG, C3, C1q, Albumin, Fibrinogen, Kappa and Lambda on acetone fixed frozen sections. These are all direct IF. We perform C4D indirectly on frozen tissue due to the lack of staining intensity of any directly conjugated antibodies, as well as C4D IF on FFPE tissue and we also perform IHC C4D on FFPE. Our pathologist's usual comments about the reason that they like the immunofluorescence is that they can easily see smaller deposits using immunofluorescence that would be indeterminate with conventional IHC. We use an automated stainer for these, it allows us to be more hands off as well as to incubate them in the dark. Let me know if you have any further questions. On Sat, May 12, 2012 at 7:48 AM, gayle callis wrote: > Dear Histonetters, > > > > I know that immunofluorescence has been done for decades on renal biopsies, > but am curious if laboratories are using IF more these days? If so, I > would be very interested to talk to you one on one about this as I have > more > questions on why you deviate from standard chromogenic enzyme > immunohistochemistry and perform IF. Comments about renal biopsy > procedures are welcome too. > > > > Also, do you do mostly single IF or double IF, and the reasons why? Is > your IF done primarily on FFPE or frozen sections/acetone fixation? > > > > When you do immunofluorescence in your clinical laboratory are you using an > automated stainer or a manual protocol? Or is your clinical laboratory > associated with medical research groups? > > > > Any comments/information is most welcome. > > > > Thanks.............. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From rory.pritchard001 <@t> gmail.com Sat May 12 12:04:38 2012 From: rory.pritchard001 <@t> gmail.com (Rory Pritchard) Date: Sat May 12 12:05:02 2012 Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 13 In-Reply-To: <4fae973a.094ab60a.1447.2819SMTPIN_ADDED@mx.google.com> References: <4fae973a.094ab60a.1447.2819SMTPIN_ADDED@mx.google.com> Message-ID: Message 3: xylene substitute for counterstain clearing. You could use histoclear, it's less toxic than xylene and works well. -Rory On May 12, 2012, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Fwd: Plants (Behnaz Sohrab) > 2. Re: Fwd: Plants (William) > 3. RE: Xylene Substitute for Counterstain Clearing (Cynthia Pyse) > 4. paraffin melting in VIP (Gudrun Lang) > 5. Re: Fwd: Plants (Victoria Baker) > 6. Re: Xylene Substitute for Counterstain Clearing (Victoria Baker) > 7. Re: Xylene Substitute for Counterstain Clearing (Rene J Buesa) > 8. Re: paraffin melting in VIP (Rene J Buesa) > 9. Re: paraffin melting in VIP (Jennifer Campbell) > 10. RE: paraffin melting in VIP (Sarah Dysart) > 11. AW: [Histonet] paraffin melting in VIP (Gudrun Lang) > 12. RE: paraffin melting in VIP (Goins, Tresa) > 13. Re: Fwd: Plants (Kim Donadio) > 14. Re: Fwd: Plants (Lucie Guernsey) > 15. RE: Xylene Substitute for Counterstain Clearing (gayle callis) > 16. RE: Fwd: Plants (Sebree Linda A) > 17. RE: Fwd: Plants (Sebree Linda A) > 18. Re: Fwd: Plants (Emily Sours) > 19. Immunofluorescence in the clinical laboratory, some questions > (gayle callis) > 20. Re: Immunofluorescence in the clinical laboratory, some > questions (Patrick Laurie) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 11 May 2012 10:29:59 -0700 > From: "Behnaz Sohrab" > Subject: [Histonet] Fwd: Plants > To: > Message-ID: <4FACEA27.4347.0054.1@ah.org> > Content-Type: text/plain; charset="us-ascii" > > > I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? > Thank you, Behnaz > > ------------------------------ > > Message: 2 > Date: Fri, 11 May 2012 13:32:37 -0400 > From: William > Subject: Re: [Histonet] Fwd: Plants > To: Behnaz Sohrab > Cc: "" > > Message-ID: <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules. > > Will Chappell > > Sent from my iPhone > > On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote: > >> >> I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? >> Thank you, Behnaz >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Fri, 11 May 2012 13:41:33 -0400 > From: "Cynthia Pyse" > Subject: RE: [Histonet] Xylene Substitute for Counterstain Clearing > To: "'Andrew Coleman'" , > > Message-ID: <001e01cd2f9d$4eaeec70$ec0cc550$@com> > Content-Type: text/plain; charset="us-ascii" > > Andrew > You could use Clearium from Leica. Clearium can either be coverslipped from > xylene or isopropyl alcohol. Drying time from isopropyl is longer then > xylene. > Cindy > > Cindy Pyse, CLT, HT (ASCP) > Laboratory Manager > X-Cell Laboratories > 716-250-9235 etx. 232 > e-mail cpyse@x-celllab.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew > Coleman > Sent: Friday, May 11, 2012 12:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitute for Counterstain Clearing > > Hi all, > > We are performing a neutral red counterstain on tissue sections containing > colored polystyrene microspheres. The spheres are inert to alcohol, but are > washed out when we clear with xylene to coverslip. > The spheres are also supposedly soluble in DMF, acetone, acetonitrile, > chloroform and methylene chloride for what its worth. > > Is it reasonable to coverslip these slides in permanent mount without > clearing with xylene after dehydrating the tissue? Or does anyone know of a > substitute clearing agent with chemical properties dissimilar enough from > xylene that might be worth trying instead? > > Thanks, > > Andrew > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 11 May 2012 19:58:39 +0200 > From: "Gudrun Lang" > Subject: [Histonet] paraffin melting in VIP > To: > Message-ID: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> > Content-Type: text/plain; charset="us-ascii" > > HI! > > A question for those, who melt the paraffin directly in the VIP. How long > does it take to melt the pellets in the VIP-oven? > > > > Thanks > > Gudrun > > > > ------------------------------ > > Message: 5 > Date: Fri, 11 May 2012 14:17:39 -0400 > From: Victoria Baker > Subject: Re: [Histonet] Fwd: Plants > To: Behnaz Sohrab > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > It's probably more toxic for the plants, but I like having them and no one > has told me I had to remove them. Ivy's are the most sturdy and the green > color just perks up things. > On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab wrote: > >> >> I was told by infectious control person that plants are not allowed in the >> lab?? IS this true? any experience with this? >> Thank you, Behnaz >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 6 > Date: Fri, 11 May 2012 14:21:45 -0400 > From: Victoria Baker > Subject: Re: [Histonet] Xylene Substitute for Counterstain Clearing > To: Andrew Coleman > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I think that it is called Crystal Mount - but apply to section, allow to > harden dip slide in clearing media and coverslip. I know that there must > be others out there as well. > Good Luck > > > > On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman > wrote: > >> Hi all, >> >> We are performing a neutral red counterstain on tissue sections >> containing colored polystyrene microspheres. The spheres are inert to >> alcohol, but are washed out when we clear with xylene to coverslip. >> The spheres are also supposedly soluble in DMF, acetone, acetonitrile, >> chloroform and methylene chloride for what its worth. >> >> Is it reasonable to coverslip these slides in permanent mount without >> clearing with xylene after dehydrating the tissue? Or does anyone know >> of a substitute clearing agent with chemical properties dissimilar >> enough from xylene that might be worth trying instead? >> >> Thanks, >> >> Andrew >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 7 > Date: Fri, 11 May 2012 11:22:57 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene Substitute for Counterstain Clearing > To: histonet@lists.utsouthwestern.edu, Andrew Coleman > > Message-ID: > <1336760577.37265.YahooMailClassic@web162101.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > After staining dry the sections in an oven at 60?C for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. > Ren? J. > > --- On Fri, 5/11/12, Andrew Coleman wrote: > > > From: Andrew Coleman > Subject: [Histonet] Xylene Substitute for Counterstain Clearing > To: histonet@lists.utsouthwestern.edu > Date: Friday, May 11, 2012, 12:25 PM > > > Hi all, > > We are performing a neutral red counterstain on tissue sections > containing colored polystyrene microspheres. The spheres are inert to > alcohol, but are washed out when we clear with xylene to coverslip. > The spheres are also supposedly soluble in DMF, acetone, acetonitrile, > chloroform and methylene chloride for what its worth. > > Is it reasonable to coverslip these slides in permanent mount without > clearing with xylene after dehydrating the tissue? Or does anyone know > of a substitute clearing agent with chemical properties dissimilar > enough from xylene that might be worth trying instead? > > Thanks, > > Andrew > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 8 > Date: Fri, 11 May 2012 11:27:36 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] paraffin melting in VIP > To: histonet@lists.utsouthwestern.edu, gu.lang@gmx.at > Message-ID: > <1336760856.98785.YahooMailClassic@web162102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!. > Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin?at the temperature you desire. > Ren? J. > > --- On Fri, 5/11/12, Gudrun Lang wrote: > > > From: Gudrun Lang > Subject: [Histonet] paraffin melting in VIP > To: histonet@lists.utsouthwestern.edu > Date: Friday, May 11, 2012, 1:58 PM > > > HI! > > A question for those, who melt the paraffin directly in the VIP. How long > does it take to melt the pellets in the VIP-oven? > > > > Thanks > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 9 > Date: Fri, 11 May 2012 14:37:21 -0400 > From: Jennifer Campbell > Subject: Re: [Histonet] paraffin melting in VIP > To: Rene J Buesa > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I agree Rene! > > I also believe Sakura recommends not putting paraffin flakes directly in > the containers. > > On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa wrote: > >> Regardless of the time it takes or of how many people do it, melting the >> paraffin directly in the VIP should not be done because it causes the >> heating elements to work extra reducing their useful life. They are quite >> expensive to replace!. >> Melt the paraffin outside the VIP and use the VIP only to keep the melted >> paraffin at the temperature you desire. >> Ren? J. >> >> --- On Fri, 5/11/12, Gudrun Lang wrote: >> >> >> From: Gudrun Lang >> Subject: [Histonet] paraffin melting in VIP >> To: histonet@lists.utsouthwestern.edu >> Date: Friday, May 11, 2012, 1:58 PM >> >> >> HI! >> >> A question for those, who melt the paraffin directly in the VIP. How long >> does it take to melt the pellets in the VIP-oven? >> >> >> >> Thanks >> >> Gudrun >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Jen Campbell, HT(ASCP) > Supervisor of Technical Services > Muhlbauer Dermatopathology Laboratory > 61 Monroe Avenue, Ste B > Pittsford NY 14534 > P: 585.586.5166 > F: 585.586.3137 > > > IMPORTANT NOTICE: This e-mail and any attachments may contain confidential > or sensitive information which is, or may be, legally privileged or > otherwise protected by law from further disclosure. It is intended only > for the addressee. If you received this in error or from someone who was > not authorized to send it to you, please do not distribute, copy or use it > or any attachments. Please notify the sender immediately by reply e-mail > and delete this from your system. Thank you for your cooperation. > > > ------------------------------ > > Message: 10 > Date: Fri, 11 May 2012 18:51:56 +0000 > From: Sarah Dysart > Subject: RE: [Histonet] paraffin melting in VIP > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > , "gu.lang@gmx.at" > Message-ID: > <8A70A9B2ECDD084DACFE6C59FCF86D50066E2135@BL2PRD0710MB363.namprd07.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > This is probably a best case scenario, but for labs like the one I am currently in I melt it in the processor. My lab doesn't have the funds to buy me a melting pot (I have had them in all my other labs, just don't know what they are technically called). > To answer your question it usually takes overnight and it's melted, but sometimes I end up having to add a little more to get to top off level, that takes a couple hours. > Good Luck!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, May 11, 2012 1:28 PM > To: histonet@lists.utsouthwestern.edu; gu.lang@gmx.at > Subject: Re: [Histonet] paraffin melting in VIP > > Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!. > Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin?at the temperature you desire. > Ren? J. > > --- On Fri, 5/11/12, Gudrun Lang wrote: > > > From: Gudrun Lang > Subject: [Histonet] paraffin melting in VIP > To: histonet@lists.utsouthwestern.edu > Date: Friday, May 11, 2012, 1:58 PM > > > HI! > > A question for those, who melt the paraffin directly in the VIP. How long > does it take to melt the pellets in the VIP-oven? > > > > Thanks > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Fri, 11 May 2012 21:31:00 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] paraffin melting in VIP > To: > Message-ID: <003b01cd2fac$98dca5d0$ca95f170$@gmx.at> > Content-Type: text/plain; charset="iso-8859-1" > > Thanks for your kind responses! > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun > Lang > Gesendet: Freitag, 11. Mai 2012 19:59 > An: histon From carl.hobbs <@t> kcl.ac.uk Sat May 12 12:21:03 2012 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat May 12 12:23:50 2012 Subject: [Histonet] Re: Immunofluorescence in the clinical laboratory, Message-ID: <11D9615B89C10747B1C985966A63D7CA3847E8545E@KCL-MAIL04.kclad.ds.kcl.ac.uk> Dear Gayle, I would be interested to know why you are interested to know ;-) Respectfully, Carl Carl Hobbs Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From gu.lang <@t> gmx.at Sat May 12 13:12:17 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat May 12 13:12:23 2012 Subject: AW: [Histonet] Immunofluorescence in the clinical laboratory, some questions In-Reply-To: <000f01cd304e$467c8380$d3758a80$@bresnan.net> References: <000f01cd304e$467c8380$d3758a80$@bresnan.net> Message-ID: <009301cd306a$c4748690$4d5d93b0$@gmx.at> Hi Gayle! We do mainly the same as Patrick. Dermatological specimens for bull. deseases on frozen air-dried slides. Direct IF + indirect IF with patients serum. We do also direct IF on NBF fixed renal biopsies, with digestion for antigen retrieval. We perform those tests manually. 2-3 skinbiopsies per week, 3-4 renal biopsies per month. Only clinical. Best regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von gayle callis Gesendet: Samstag, 12. Mai 2012 16:48 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Immunofluorescence in the clinical laboratory, some questions Dear Histonetters, I know that immunofluorescence has been done for decades on renal biopsies, but am curious if laboratories are using IF more these days? If so, I would be very interested to talk to you one on one about this as I have more questions on why you deviate from standard chromogenic enzyme immunohistochemistry and perform IF. Comments about renal biopsy procedures are welcome too. Also, do you do mostly single IF or double IF, and the reasons why? Is your IF done primarily on FFPE or frozen sections/acetone fixation? When you do immunofluorescence in your clinical laboratory are you using an automated stainer or a manual protocol? Or is your clinical laboratory associated with medical research groups? Any comments/information is most welcome. Thanks.............. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vrivera <@t> westderm.com Sat May 12 13:36:07 2012 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Sat May 12 13:36:25 2012 Subject: [Histonet] Histology / Grossing position(s) in Placentia, CA Message-ID: Hello fellow Histonet community, West Dermatology Pathology Laboratory, located in Placentia California has the following position(s) open: A growing dermatopathology laboratory is looking for qualified, certified or certification eligible, Histotechnician and Histotechnologists to start immediately. Come be a part of our team. Competitive salary and benefits. Growth opportunities and leadership ancillary duties possible based your education and training. Please do not apply if you are not nationally certified or certification eligible. Grossing room experience preferred. 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Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From ibernard <@t> uab.edu Sun May 13 11:14:42 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun May 13 11:14:59 2012 Subject: [Histonet] Tissue Processing Protocol for Small iopsies Message-ID: Fellow histonetters, I'm looking for evidence based or best practice/ benchmarked tissue processing protocols for the small biopsies listed below. Please provide a reference since our facility strives for evidence based procedures for our patients . I am processing a number of: - Endoscopic or gastroenterology biopsies - Breast Core Biopsies Currently, owing to one processor (Sakura, Tissue Tek- VIP-5), we put all tissues on our 12 hour processing run. As a result, the endoscopic tissue, owing to its size, tends to be more dehydrated. To avoid the chatter artifacts associated with over dehydration, we soak these specimen blocks, a minimum of 15 minutes before sectioning with minimal or no chatter. We are hoping to improving turnaround time( to the pathologist) by not having to soak so long. Thus, we feel that a tissue processing protocol of less time will make a difference. As we all know CAP and ASCO, have recommended breast core biopsies (the evidence based standard for determining breast cancer) be fixed at a minimum of 6 to 72 hrs before processing to accommodate accurate testing of immune stains ER. PR and Her2Neu for breast cancer determination. Our processor reagents are as follows: 10% Neutral Buffered Formalin x 2 changes; 70% isopropanol x 1; 95% isopropanol x 1; 100% isopropanol- x 3 changes; clearing reagent- x 3 changes; and paraffin- 4 changes. By the way, we are looking at purchasing another processor- my next communicated topic to accommodate processing of our small biopsies. Question: is it ok to mix endoscopic with other small tissues such as: cervical bxs, shaves, ECC or EMB specimens on the same endoscopic processing protocol? Again provide a reference as a matter of evidence based medicine. We do this to try and split cases to avoid cross contamination at the grossing, embedding and cutting. We consider this a QA mechanism for maintaining the integrity of the specimen cases. V/r Ian R. Bernard Ian R. Bernard, MSgt, USAF, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From b427297 <@t> aol.com Sun May 13 14:42:16 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Sun May 13 14:42:25 2012 Subject: [Histonet] Can use some help with processing references In-Reply-To: References: Message-ID: <8CEFF57A2C5E94C-7FC-76B33@webmail-m029.sysops.aol.com> Hi Y'all - I'm kind of in a jam here - I'm looking for references on paraffin tissue processing - can someone point me towards any literature that says leaving tissues in molten paraffin over the weekend is a bad idea? I need to prove a point, and need something to back me up. I'm out of the US doing a consult, having trouble doing a lit search - I'm really not lazy. Thanks in advance. Jackie O' From ibernard <@t> uab.edu Sun May 13 16:48:42 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun May 13 16:48:54 2012 Subject: [Histonet] Can use some help with processing references In-Reply-To: <8CEFF57A2C5E94C-7FC-76B33@webmail-m029.sysops.aol.com> References: <8CEFF57A2C5E94C-7FC-76B33@webmail-m029.sysops.aol.com> Message-ID: Rather than research literature (lit), may I refer you to the foremost authority on histotechnology procedures: Histotechonology- A Self Instructional Text, 3rd Edition by Frieda Carson and Christa Hladik, Chapter 2, page 38, paragraph 2: "Tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening." I consider book the foremost (bible) reference on histotechnology. At least I hope so, since I'm studying for my HTL from this book. Note: There are lit at the end of this chapter that may go into more detail. The paragraph goes on to state that: " ...melted paraffin should be kept 2 to 4 degrees Celsius above the melting point because tissues exposed to overheated paraffin during infiltration will over harden. Bottom-line, I would not leave tissues in paraffin over the weekend. If you are using an automatic-closed system processor, your equipment should be able to work on a delayed status, where the tissues will sit in 10 Neutral Buffered Formalin under vacuum (better for the tissue), all weekend, and start processing schedule on Sunday to come off on Monday morning. At least ours does. Hope this helps. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Sunday, May 13, 2012 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can use some help with processing references Hi Y'all - I'm kind of in a jam here - I'm looking for references on paraffin tissue processing - can someone point me towards any literature that says leaving tissues in molten paraffin over the weekend is a bad idea? I need to prove a point, and need something to back me up. I'm out of the US doing a consult, having trouble doing a lit search - I'm really not lazy. Thanks in advance. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Sun May 13 17:20:13 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun May 13 17:20:28 2012 Subject: [Histonet] RE: Tissue Processing Protocol for Small biopsies part 2 Message-ID: Regarding the fixation time for breast biopsies, after doing some more research, I stand corrected (not 72 but up to 18hrs.) with the maximum amount of time for fixation in order to preserve the ER antigen in immunohistochemistry. See this paragraph taken from CAP website: "The type of fixative also has an impact on the results of estrogen receptor determination. Most labs use 10 percent buffered formalin. It's a great general fixative. It doesn't make the tissue too hard, and it fixes tissue reasonably quickly. Optimum fixation time for estrogen receptor activity is between six and 18 hours for buffered formalin. Less than that and more than that, you're going to get a diminution in estrogen receptor or even a false-negative. The optimum fixation time also includes processor time. Consider your tissue is sitting in a processor over the weekend in 10 percent buffered formalin for up to 48 hours. You may well end up with false-negative reactivity or at least lowered estrogen receptor reactivity in breast cancer. Too little fixation is also a problem. It is a problem that I encountered in testing for estrogen receptor on breast cores. Our breast cores on the biopsy processor were getting a total of eight to 10 hours of fixation. The laboratory changed its procedure and started processing immediately after loading biopsy tissue. All of a sudden my breast cores were getting only two to six hours of fixation. And there was a falloff in estrogen receptor activity." Though I'd double check myself. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From: Ian R Bernard Sent: Sunday, May 13, 2012 11:15 AM To: 'histonet@lists.utsouthwestern.edu' Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH Subject: Tissue Processing Protocol for Small iopsies Fellow histonetters, I'm looking for evidence based or best practice/ benchmarked tissue processing protocols for the small biopsies listed below. Please provide a reference since our facility strives for evidence based procedures for our patients . I am processing a number of: - Endoscopic or gastroenterology biopsies - Breast Core Biopsies Currently, owing to one processor (Sakura, Tissue Tek- VIP-5), we put all tissues on our 12 hour processing run. As a result, the endoscopic tissue, owing to its size, tends to be more dehydrated. To avoid the chatter artifacts associated with over dehydration, we soak these specimen blocks, a minimum of 15 minutes before sectioning with minimal or no chatter. We are hoping to improving turnaround time( to the pathologist) by not having to soak so long. Thus, we feel that a tissue processing protocol of less time will make a difference. As we all know CAP and ASCO, have recommended breast core biopsies (the evidence based standard for determining breast cancer) be fixed at a minimum of 6 to 72 hrs before processing to accommodate accurate testing of immune stains ER. PR and Her2Neu for breast cancer determination. Our processor reagents are as follows: 10% Neutral Buffered Formalin x 2 changes; 70% isopropanol x 1; 95% isopropanol x 1; 100% isopropanol- x 3 changes; clearing reagent- x 3 changes; and paraffin- 4 changes. By the way, we are looking at purchasing another processor- my next communicated topic to accommodate processing of our small biopsies. Question: is it ok to mix endoscopic with other small tissues such as: cervical bxs, shaves, ECC or EMB specimens on the same endoscopic processing protocol? Again provide a reference as a matter of evidence based medicine. We do this to try and split cases to avoid cross contamination at the grossing, embedding and cutting. We consider this a QA mechanism for maintaining the integrity of the specimen cases. V/r Ian R. Bernard Ian R. Bernard, MSgt, USAF, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From ibernard <@t> uab.edu Sun May 13 17:31:19 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun May 13 17:31:25 2012 Subject: [Histonet] New Topic: New Tissue Processor Message-ID: We are looking at purchasing the Leica ASP 300S tissue processor. Like I mentioned below, I have the Sakura Tissue Tek VIP 5. Thus for my purchase package, I'm looking for references (pros and cons), of your experiences with the Leica ASP 300s. So far, I've heard good things. In particular, if there are any military or Federal agencies out there with this processor, please respond. However, I will take all responses. Note: This is no Federal endorsement of the Leica or Sakura processors, just our lab's preference based upon experiences/feedback. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From: Ian R Bernard Sent: Sunday, May 13, 2012 11:15 AM To: 'histonet@lists.utsouthwestern.edu' Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH Subject: Tissue Processing Protocol for Small iopsies Fellow histonetters, I'm looking for evidence based or best practice/ benchmarked tissue processing protocols for the small biopsies listed below. Please provide a reference since our facility strives for evidence based procedures for our patients . I am processing a number of: - Endoscopic or gastroenterology biopsies - Breast Core Biopsies Currently, owing to one processor (Sakura, Tissue Tek- VIP-5), we put all tissues on our 12 hour processing run. As a result, the endoscopic tissue, owing to its size, tends to be more dehydrated. To avoid the chatter artifacts associated with over dehydration, we soak these specimen blocks, a minimum of 15 minutes before sectioning with minimal or no chatter. We are hoping to improving turnaround time( to the pathologist) by not having to soak so long. Thus, we feel that a tissue processing protocol of less time will make a difference. As we all know CAP and ASCO, have recommended breast core biopsies (the evidence based standard for determining breast cancer) be fixed at a minimum of 6 to 72 hrs before processing to accommodate accurate testing of immune stains ER. PR and Her2Neu for breast cancer determination. Our processor reagents are as follows: 10% Neutral Buffered Formalin x 2 changes; 70% isopropanol x 1; 95% isopropanol x 1; 100% isopropanol- x 3 changes; clearing reagent- x 3 changes; and paraffin- 4 changes. By the way, we are looking at purchasing another processor- my next communicated topic to accommodate processing of our small biopsies. Question: is it ok to mix endoscopic with other small tissues such as: cervical bxs, shaves, ECC or EMB specimens on the same endoscopic processing protocol? Again provide a reference as a matter of evidence based medicine. We do this to try and split cases to avoid cross contamination at the grossing, embedding and cutting. We consider this a QA mechanism for maintaining the integrity of the specimen cases. V/r Ian R. Bernard Ian R. Bernard, MSgt, USAF, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From ibernard <@t> uab.edu Sun May 13 17:55:27 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun May 13 17:55:39 2012 Subject: [Histonet] Tendon Tissue Specimens In-Reply-To: References: Message-ID: Histonetters, our lab processes tendon tissue. After processing, embedding and at the microtome, the specimen tends to be hard (possible calcification) like bone. We get decent sections but not the best, consistently. To help get the decent sections, we soak longer than regular tissues (almost like the endoscopic tissues), and we do some surface decalcifying. Besides these two techniques, are there are other methods for obtaining optimum sections (I.e. full face and complete), e.g. processing protocol, soak in another reagent etc.? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From tony.henwood <@t> health.nsw.gov.au Sun May 13 18:38:36 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun May 13 18:39:00 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <4FACEA27.4347.0054.1@ah.org> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A48E7C@xmdb02.nch.kids> If referring to a Histopathology lab, what a load of codswallop. Don't we love supposed Professionals who make decisions based on wrong information. I would suggest that the "Infectious Control person" do some further study on other areas of pathology. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Saturday, 12 May 2012 3:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Sun May 13 18:42:52 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun May 13 18:43:13 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <1336766842.44972.YahooMailNeo@web112312.mail.gq1.yahoo.com> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA@yahoo.com> <1336766842.44972.YahooMailNeo@web112312.mail.gq1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A48EAC@xmdb02.nch.kids> I would suggest that it is the potting mix that is the culprit not the plant. But then remember Histo laboratories are not ICUs nor are they Microbiology laboratories. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Saturday, 12 May 2012 6:07 AM To: William; Behnaz Sohrab Cc: Subject: Re: [Histonet] Fwd: Plants Hate to say it, but yes plants are considered infectious. Thats why you cant take them in ICU's either. I guess the mold or bacterias can grow on them. Most places let this slide, but some dont. Good luck! ________________________________ From: William To: Behnaz Sohrab Cc: "" Sent: Friday, May 11, 2012 1:32 PM Subject: Re: [Histonet] Fwd: Plants I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules. Will Chappell Sent from my iPhone On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" wrote: > > I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? > Thank you, Behnaz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From b427297 <@t> aol.com Mon May 14 01:07:10 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Mon May 14 01:07:15 2012 Subject: [Histonet] Can use some help with processing references In-Reply-To: References: <8CEFF57A2C5E94C-7FC-76B33@webmail-m029.sysops.aol.com> Message-ID: <8CEFFAEEEEC5C6D-2010-40161@webmail-d002.sysops.aol.com> Thank you, Ian - the quote from Carson's book with be most helpful. I do not have access to reference texts in my current location - I very much appreciate your time to provide the quote. Good luck with your HTL! -----Original Message----- From: Ian R Bernard To: Jackie O'Connor ; histonet Sent: Sun, May 13, 2012 4:48 pm Subject: RE: [Histonet] Can use some help with processing references Rather than research literature (lit), may I refer you to the foremost authority n histotechnology procedures: Histotechonology- A Self Instructional Text, 3rd dition by Frieda Carson and Christa Hladik, Chapter 2, page 38, paragraph 2: Tissue should remain in paraffin the shortest time necessary for good nfiltration because exposure to prolonged heat causes shrinkage and hardening." consider book the foremost (bible) reference on histotechnology. At least I ope so, since I'm studying for my HTL from this book. Note: There are lit at he end of this chapter that may go into more detail. The paragraph goes on to state that: " ...melted paraffin should be kept 2 to 4 egrees Celsius above the melting point because tissues exposed to overheated araffin during infiltration will over harden. Bottom-line, I would not leave tissues in paraffin over the weekend. If you are sing an automatic-closed system processor, your equipment should be able to ork on a delayed status, where the tissues will sit in 10 Neutral Buffered ormalin under vacuum (better for the tissue), all weekend, and start processing chedule on Sunday to come off on Monday morning. At least ours does. Hope this helps. Ian R. Bernard an R. Bernard, MSHA, HT (ASCP) 0th Medical Group- Anatomic Pathology Lab SAF Academy, CO 80840 -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Jackie O'Connor ent: Sunday, May 13, 2012 2:42 PM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Can use some help with processing references Hi Y'all - I'm kind of in a jam here - I'm looking for references on paraffin issue processing - can someone point me towards any literature that says eaving tissues in molten paraffin over the weekend is a bad idea? I need to rove a point, and need something to back me up. I'm out of the US doing a onsult, having trouble doing a lit search - I'm really not lazy. hanks in advance. ackie O' ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Mon May 14 02:25:05 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon May 14 02:25:27 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DEA25AD3B@FWDCWPMSGCMS09.hca.corpad.net> I read an article once that said spider plants absorb formalin fumes we have kept them in the histo lab for years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Friday, May 11, 2012 4:07 PM To: Victoria Baker Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab Subject: Re: [Histonet] Fwd: Plants I keep a pothos and spider plant in my lab. EH&S has never complained, though I can't say one way or another if it's technically allowed. While my plants are mostly just decorative (I don't think I have enough of them to make much of a difference), it doesn't hurt that they may be filtering our air somewhat. NASA compiled a list of air-filtering plants that can eliminate significant amounts of formaldehyde, xylene, benzene, etc. (Source: http://en.wikipedia.org/wiki/List_of_air-filtering_plants). Lucie UCSD Dept. of Pathology On Friday, May 11, 2012, Victoria Baker wrote: > It's probably more toxic for the plants, but I like having them and no one > has told me I had to remove them. Ivy's are the most sturdy and the green > color just perks up things. > On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab wrote: > > > > > I was told by infectious control person that plants are not allowed in > the > > lab?? IS this true? any experience with this? > > Thank you, Behnaz > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamcas1 <@t> gmail.com Mon May 14 04:32:51 2012 From: jamcas1 <@t> gmail.com (James Castillo) Date: Mon May 14 04:33:00 2012 Subject: [Histonet] Southern California Message-ID: Hello Histonet! I am posting for a friend that is looking to relocate to the San Diego area. Does anyone know of any open histology positions that might not already be posted job hunting websites like NSH, Careerbuilder or Indeed? He is an HT certified tech and has CLIA qualifications to gross. Thanks to anyone who can help and have a great week! From cdemarinis <@t> SARATOGACARE.ORG Mon May 14 06:28:44 2012 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Mon May 14 06:28:49 2012 Subject: [Histonet] IRON STAIN Message-ID: <1C75A843982A7B44BB368A3CC946ABCA0EB00F@SHEXCHMBX01.SARAHOSP.ORG> Our iron stain (Newcomer kit) works great for tissue sections but is not adequate for identifying sideroblasts in a bone marrow film. What is the preferred iron stain to identify sideroblasts and which company do you purchase the kit from? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From one_angel_secret <@t> yahoo.com Mon May 14 07:03:36 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon May 14 07:03:54 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DEA25AD3B@FWDCWPMSGCMS09.hca.corpad.net> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> <4BF03F5404EBDE409AF9232DA74B9DED2DEA25AD3B@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <28D65C00-C1F6-45B3-999A-C444DCCDC033@yahoo.com> Hey I love having plants in the lab. I just offered up what I've been told by other infectious control people. Some infectious control people are more serious than others. In the end you'll have to sell that person on it. I've seen it turn out both ways. Happy week ! Kim D Sent from my iPhone On May 14, 2012, at 3:25 AM, wrote: > I read an article once that said spider plants absorb formalin fumes we have kept them in the histo lab for years. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey > Sent: Friday, May 11, 2012 4:07 PM > To: Victoria Baker > Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab > Subject: Re: [Histonet] Fwd: Plants > > I keep a pothos and spider plant in my lab. EH&S has never complained, > though I can't say one way or another if it's technically allowed. > > While my plants are mostly just decorative (I don't think I have enough of > them to make much of a difference), it doesn't hurt that they may be > filtering our air somewhat. NASA compiled a list of air-filtering plants > that can eliminate significant amounts of formaldehyde, xylene, benzene, > etc. (Source: http://en.wikipedia.org/wiki/List_of_air-filtering_plants). > > Lucie > UCSD > Dept. of Pathology > > > On Friday, May 11, 2012, Victoria Baker wrote: > >> It's probably more toxic for the plants, but I like having them and no one >> has told me I had to remove them. Ivy's are the most sturdy and the green >> color just perks up things. >> On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab wrote: >> >>> >>> I was told by infectious control person that plants are not allowed in >> the >>> lab?? IS this true? any experience with this? >>> Thank you, Behnaz >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Mon May 14 07:10:43 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Mon May 14 07:10:49 2012 Subject: [Histonet] RE: Tissue Processing Protocol for Small iopsies In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB762460BAD35E1@TN001WEXMBX12.US.chs.net> Ian, For our endoscopic biopsies a 12 hour run is too long. We load ours in the afternoon but they stay in formalin until about 2 am. They end at 5:30 in the morning. The dehydration is 20 minutes x5 stations, with 30 in the 6th. Clearant is 20 minutes x2 stations, with 30 in the 3rd. Paraffin is 30 min. x2 and 45 in the 3rd station. Our breast cores process with our larger tissues for the 12 hr. program without advise affects. However we use ProSoft and ProPar from Anatech (on both processors) which is very gentle on the tissues. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Sunday, May 13, 2012 12:15 PM To: histonet@lists.utsouthwestern.edu Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH Subject: [Histonet] Tissue Processing Protocol for Small iopsies Fellow histonetters, I'm looking for evidence based or best practice/ benchmarked tissue processing protocols for the small biopsies listed below. Please provide a reference since our facility strives for evidence based procedures for our patients . I am processing a number of: - Endoscopic or gastroenterology biopsies - Breast Core Biopsies Currently, owing to one processor (Sakura, Tissue Tek- VIP-5), we put all tissues on our 12 hour processing run. As a result, the endoscopic tissue, owing to its size, tends to be more dehydrated. To avoid the chatter artifacts associated with over dehydration, we soak these specimen blocks, a minimum of 15 minutes before sectioning with minimal or no chatter. We are hoping to improving turnaround time( to the pathologist) by not having to soak so long. Thus, we feel that a tissue processing protocol of less time will make a difference. As we all know CAP and ASCO, have recommended breast core biopsies (the evidence based standard for determining breast cancer) be fixed at a minimum of 6 to 72 hrs before processing to accommodate accurate testing of immune stains ER. PR and Her2Neu for breast cancer determination. Our processor reagents are as follows: 10% Neutral Buffered Formalin x 2 changes; 70% isopropanol x 1; 95% isopropanol x 1; 100% isopropanol- x 3 changes; clearing reagent- x 3 changes; and paraffin- 4 changes. By the way, we are looking at purchasing another processor- my next communicated topic to accommodate processing of our small biopsies. Question: is it ok to mix endoscopic with other small tissues such as: cervical bxs, shaves, ECC or EMB specimens on the same endoscopic processing protocol? Again provide a reference as a matter of evidence based medicine. We do this to try and split cases to avoid cross contamination at the grossing, embedding and cutting. We consider this a QA mechanism for maintaining the integrity of the specimen cases. V/r Ian R. Bernard Ian R. Bernard, MSgt, USAF, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From stephanie.d.rivera <@t> gsk.com Mon May 14 08:10:33 2012 From: stephanie.d.rivera <@t> gsk.com (Stephanie Rivera) Date: Mon May 14 08:10:50 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <4FACEA27.4347.0054.1@ah.org> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> Message-ID: <0D4094F105C6A74B87960B0746967965A031D7@019-SN2MPN1-041.019D.MGD.MSFT.NET> We were told last year to remove all plants from our lab........something about contaminants.....not sure if it was FDA rule or our department rule, but we had to remove all plants and an inspection from the higher ups was done to ensure all plants had been removed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, May 11, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz From TJohnson <@t> gnf.org Mon May 14 10:14:52 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon May 14 10:15:02 2012 Subject: [Histonet] Re: Immunofluorescence in the clinical laboratory Message-ID: <9F3CFEE76E51B64991C7485270890B4009F3204D@EX5.lj.gnf.org> To those doing DIF in the clinical lab, are you using frozen tonsils or other tissue as control material when you do these? Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From mbmphoto <@t> gmail.com Mon May 14 10:17:53 2012 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon May 14 10:17:59 2012 Subject: [Histonet] histologist seeking recruiters Message-ID: Hello, I've recently been laid off from UCSF due to lack of funding. Would histology recruiters for the Bay Area in Northern California, please contact me on my personal email address mbmphoto@gmail.com Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF SF CA 94103 From hymclab.hymclab <@t> ministryhealth.org Mon May 14 10:22:05 2012 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Mon May 14 10:22:20 2012 Subject: [Histonet] paraffin melting in VIP In-Reply-To: References: <001b01cd2f9f$b24a4610$16ded230$@gmx.at> Message-ID: We do the same thing Tresa. I've been here 20 years and have an original VIP that is 22 years old as our back up and a VIP 5 that is 8 years old as our primary and have never had 1 issue with the heating elements!! Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, May 11, 2012 2:33 PM To: 'gu.lang@gmx.at'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] paraffin melting in VIP When it is time to exchange reagents, the paraffin is the first thing we do because of the melting time required. We fill up the bin and place it in the oven until there is room to add more wax pellets - we do not stuff it in, but leave it rather loose. We do this about six times during the day until the bin is full. The paraffin pellets are not "cold" and there is a lot of residual heat in the oven due to the three hot wax bins already at temperature, so I am not sure how much "stress" this places on the heating elements. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, May 11, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin melting in VIP HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From jessica <@t> prometheushealthcare.com Mon May 14 10:44:53 2012 From: jessica <@t> prometheushealthcare.com (Jessica-Prometheus) Date: Mon May 14 10:45:02 2012 Subject: [Histonet] Histology Supervisor Opening in AZ! Message-ID: <006b01cd31e8$815fb800$841f2800$@prometheushealthcare.com> Hey, I specialize in the recruitment of lab professionals in Arizona, and I'm looking to fill a full time, day time Histology Supervisor position available just north of Phoenix. It offers a great salary and full benefits. If you are interested or know anyone who might be, please contact me. Please feel free to pass along this information as well. For immediate consideration, email me your most up to date resume. Thank you! Jessica Sanchez Account Manager Fax 301-368-2478 jessica@prometheushealthcare.com www.prometheushealthcare.com http://twitter.com/PrometheusBlog Click Here to Meet Me! From LSebree <@t> uwhealth.org Mon May 14 11:53:48 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon May 14 11:53:52 2012 Subject: [Histonet] Re: Immunofluorescence in the clinical laboratory In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F3204D@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F3204D@EX5.lj.gnf.org> Message-ID: Yes as well as the tissue usually run with these antibodies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Monday, May 14, 2012 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Immunofluorescence in the clinical laboratory To those doing DIF in the clinical lab, are you using frozen tonsils or other tissue as control material when you do these? Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Mon May 14 13:13:11 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon May 14 13:15:27 2012 Subject: [Histonet] Can use some help with processing references References: <8CEFF57A2C5E94C-7FC-76B33@webmail-m029.sysops.aol.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A843@UWHC-MAIL2.uwhis.hosp.wisc.edu> We have also removed the blocks and let them harden until we are ready to embed them, then put back in the hot well on the embedder to warm back up and go from there. As they are already processed and infiltrated, I don't think there would be a problem leaving them at room temperature for a few days. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ian R Bernard Sent: Sun 5/13/2012 4:48 PM To: Jackie O'Connor; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Can use some help with processing references Rather than research literature (lit), may I refer you to the foremost authority on histotechnology procedures: Histotechonology- A Self Instructional Text, 3rd Edition by Frieda Carson and Christa Hladik, Chapter 2, page 38, paragraph 2: "Tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening." I consider book the foremost (bible) reference on histotechnology. At least I hope so, since I'm studying for my HTL from this book. Note: There are lit at the end of this chapter that may go into more detail. The paragraph goes on to state that: " ...melted paraffin should be kept 2 to 4 degrees Celsius above the melting point because tissues exposed to overheated paraffin during infiltration will over harden. Bottom-line, I would not leave tissues in paraffin over the weekend. If you are using an automatic-closed system processor, your equipment should be able to work on a delayed status, where the tissues will sit in 10 Neutral Buffered Formalin under vacuum (better for the tissue), all weekend, and start processing schedule on Sunday to come off on Monday morning. At least ours does. Hope this helps. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Sunday, May 13, 2012 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can use some help with processing references Hi Y'all - I'm kind of in a jam here - I'm looking for references on paraffin tissue processing - can someone point me towards any literature that says leaving tissues in molten paraffin over the weekend is a bad idea? I need to prove a point, and need something to back me up. I'm out of the US doing a consult, having trouble doing a lit search - I'm really not lazy. Thanks in advance. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Mon May 14 13:35:12 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon May 14 13:35:21 2012 Subject: [Histonet] RE: Plants Message-ID: Everywhere I have worked we had plants. Old fashioned Ivy's and a spider plant. The spider plant was for the fumes, and the Ivy was because they grew under any conditions. I have worked in veterinary, and human labs and have never had plants be a big deal. Even CAP never said anything. Toysha ------------------------------ Message: 7 Date: Sun, 13 May 2012 23:42:52 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] Fwd: Plants To: "'Kim Donadio'" , William , Behnaz Sohrab Cc: "" Message-ID: <6D6BD1DE8A5571489398B392A38A715760A48EAC@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" I would suggest that it is the potting mix that is the culprit not the plant. But then remember Histo laboratories are not ICUs nor are they Microbiology laboratories. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Saturday, 12 May 2012 6:07 AM To: William; Behnaz Sohrab Cc: Subject: Re: [Histonet] Fwd: Plants Hate to say it, but yes plants are considered infectious. Thats why you cant take them in ICU's either. I guess the mold or bacterias can grow on them. Most places let this slide, but some dont. Good luck! Message: 9 Date: Mon, 14 May 2012 02:25:05 -0500 From: Subject: RE: [Histonet] Fwd: Plants To: , Cc: histonet@lists.utsouthwestern.edu, SohrabB1@ah.org Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DEA25AD3B@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" I read an article once that said spider plants absorb formalin fumes we have kept them in the histo lab for years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Friday, May 11, 2012 4:07 PM To: Victoria Baker Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab Subject: Re: [Histonet] Fwd: Plants I keep a pothos and spider plant in my lab. EH&S has never complained, though I can't say one way or another if it's technically allowed. While my plants are mostly just decorative (I don't think I have enough of them to make much of a difference), it doesn't hurt that they may be filtering our air somewhat. NASA compiled a list of air-filtering plants that can eliminate significant amounts of formaldehyde, xylene, benzene, etc. (Source: http://en.wikipedia.org/wiki/List_of_air-filtering_plants). Lucie UCSD Dept. of Pathology Message: 12 Date: Mon, 14 May 2012 08:03:36 -0400 From: Kim Donadio Subject: Re: [Histonet] Fwd: Plants To: "" Cc: "histonet@lists.utsouthwestern.edu" , "SohrabB1@ah.org" , "" Message-ID: <28D65C00-C1F6-45B3-999A-C444DCCDC033@yahoo.com> Content-Type: text/plain; charset=us-ascii Hey I love having plants in the lab. I just offered up what I've been told by other infectious control people. Some infectious control people are more serious than others. In the end you'll have to sell that person on it. I've seen it turn out both ways. Happy week ! Kim D Message: 14 Date: Mon, 14 May 2012 13:10:33 +0000 From: Stephanie Rivera Subject: RE: [Histonet] Fwd: Plants To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <0D4094F105C6A74B87960B0746967965A031D7@019-SN2MPN1-041.019D.MGD.MSFT.NET> Content-Type: text/plain; charset="us-ascii" We were told last year to remove all plants from our lab........something about contaminants.....not sure if it was FDA rule or our department rule, but we had to remove all plants and an inspection from the higher ups was done to ensure all plants had been removed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, May 11, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz From POWELL_SA <@t> mercer.edu Mon May 14 14:50:30 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon May 14 14:50:35 2012 Subject: [Histonet] Histo position available in Georgia Message-ID: <9BF995BC0E47744E9673A41486E24EE24B36E53EBD@MERCERMAIL.MercerU.local> Posting for a friend. Please respond to the below contact information. Histotechnician/technologist position available at HCA Coliseum Medical Centers, Macon, GA. Full-time. Monday-Friday. At least one year experience preferred. Apply on line at www.coliseumhealthsytem.com or call Redonna Bunch at 478-464-5486. From rjbuesa <@t> yahoo.com Mon May 14 14:54:00 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 14 14:54:04 2012 Subject: [Histonet] RE: Plants In-Reply-To: Message-ID: <1337025240.36169.YahooMailClassic@web162101.mail.bf1.yahoo.com> If you go to Wikipedia there is an extensive list of plants and which chemicals they absorb so, at the end of the day, plants may turn to be advisable to have in our highly dangerous environment. Ren? J. --- On Mon, 5/14/12, Mayer,Toysha N wrote: From: Mayer,Toysha N Subject: [Histonet] RE: Plants To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, May 14, 2012, 2:35 PM Everywhere I have worked we had plants.? Old fashioned Ivy's and a spider plant.? The spider plant was for the fumes, and the Ivy was because they grew under any conditions. I have worked in veterinary, and human labs and have never had plants be a big deal. Even CAP never said anything. Toysha ------------------------------ Message: 7 Date: Sun, 13 May 2012 23:42:52 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] Fwd: Plants To: "'Kim Donadio'" , William ??? , ??? Behnaz Sohrab Cc: "" ??? Message-ID: <6D6BD1DE8A5571489398B392A38A715760A48EAC@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" I would suggest that it is the potting mix that is the culprit not the plant. But then remember Histo laboratories are not ICUs nor are they Microbiology laboratories. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Saturday, 12 May 2012 6:07 AM To: William; Behnaz Sohrab Cc: Subject: Re: [Histonet] Fwd: Plants Hate to say it, but yes plants are considered infectious. Thats why you cant take them in ICU's either. I guess the mold or bacterias can grow on them. Most places let this slide, but some dont. Good luck! Message: 9 Date: Mon, 14 May 2012 02:25:05 -0500 From: Subject: RE: [Histonet] Fwd: Plants To: , Cc: histonet@lists.utsouthwestern.edu, SohrabB1@ah.org Message-ID: ??? <4BF03F5404EBDE409AF9232DA74B9DED2DEA25AD3B@FWDCWPMSGCMS09.hca.corpad.net> ??? Content-Type: text/plain; charset="us-ascii" I read an article once that said spider plants absorb formalin fumes we have kept them in the histo lab for years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Friday, May 11, 2012 4:07 PM To: Victoria Baker Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab Subject: Re: [Histonet] Fwd: Plants I keep a pothos and spider plant in my lab. EH&S has never complained, though I can't say one way or another if it's technically allowed. While my plants are mostly just decorative (I don't think I have enough of them to make much of a difference), it doesn't hurt that they may be filtering our air somewhat. NASA compiled a list of air-filtering plants that can eliminate significant amounts of formaldehyde, xylene, benzene, etc. (Source:???http://en.wikipedia.org/wiki/List_of_air-filtering_plants). Lucie UCSD Dept. of Pathology Message: 12 Date: Mon, 14 May 2012 08:03:36 -0400 From: Kim Donadio Subject: Re: [Histonet] Fwd: Plants To: "" ??? Cc: "histonet@lists.utsouthwestern.edu" ??? ,??? "SohrabB1@ah.org" ??? ,??? "" Message-ID: <28D65C00-C1F6-45B3-999A-C444DCCDC033@yahoo.com> Content-Type: text/plain;??? charset=us-ascii Hey I love having plants in the lab. I just offered up what I've been told by other infectious control people. Some infectious control people are more serious than others. In the end you'll have to sell that person on it. I've seen it turn out both ways. Happy week ! Kim D Message: 14 Date: Mon, 14 May 2012 13:10:33 +0000 From: Stephanie Rivera Subject: RE: [Histonet] Fwd: Plants To: Behnaz Sohrab , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <0D4094F105C6A74B87960B0746967965A031D7@019-SN2MPN1-041.019D.MGD.MSFT.NET> ??? Content-Type: text/plain; charset="us-ascii" We were told last year to remove all plants from our lab........something about contaminants.....not sure if it was FDA rule or our department rule, but we had to remove all plants and an inspection from the higher ups was done to ensure all plants had been removed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, May 11, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon May 14 18:24:06 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon May 14 18:24:26 2012 Subject: [Histonet] Fwd: Plants In-Reply-To: <0D4094F105C6A74B87960B0746967965A031D7@019-SN2MPN1-041.019D.MGD.MSFT.NET> References: <4FABE0A2.4347.0054.1@ah.org> <4FACEA27.4347.0054.1@ah.org> <0D4094F105C6A74B87960B0746967965A031D7@019-SN2MPN1-041.019D.MGD.MSFT.NET> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A492AA@xmdb02.nch.kids> Well, Why are we not surprised that the "higher-ups" have the authority but little knowledge? At least Stephanie's Bosses decisions cannot be taken as gospel for the rest of us! (ie we can ignore them) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephanie Rivera Sent: Monday, 14 May 2012 11:11 PM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fwd: Plants We were told last year to remove all plants from our lab........something about contaminants.....not sure if it was FDA rule or our department rule, but we had to remove all plants and an inspection from the higher ups was done to ensure all plants had been removed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, May 11, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Diane.Tokugawa <@t> kp.org Mon May 14 18:41:44 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Mon May 14 18:42:06 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 05/14/2012 and will not return until 06/06/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, IHC/Histo issues Kiran at 8-421-5404, or general histology client service at 8-421-5408. I will have limited access to Lotus Notes. From bhartologist <@t> gmail.com Mon May 14 21:59:07 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Mon May 14 21:59:11 2012 Subject: [Histonet] Looking for work in Bay Area Message-ID: Hello all! You'll be happy to know (maybe lol) I have officially scheduled to take my ASCP Exam on June 11th and will be hitting the road to Berkeley, CA on June 12th, pass or fail, preferably pass of course :) So I am HT exam eligible if anyone knows of any Histotech jobs within BART distance of Berkeley. I have a car too so it doesn't have to be just within BART distance. Thanks to anyone who can help! :) Sincerley, Bharti From JMacDonald <@t> mtsac.edu Tue May 15 01:06:58 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue May 15 01:07:54 2012 Subject: [Histonet] Looking for work in Bay Area In-Reply-To: References: Message-ID: [1]http://www.californiahistology.org/job_postings/jobs.html There are a couple of jobs in Berkeley poste -----histonet-bounces@li To: Histonet Archive upei.ca Tue May 15 06:51:44 2012 From: Mhorne <@t> upei.ca (Margaret Horne) Date: Tue May 15 06:51:55 2012 Subject: [Histonet] plants Message-ID: <4FB21920020000D100013807@oes-grpwise.novell.upei.ca> I had to remove my plants from a lab because people were doing something ( RNAse ? ) that could be contaminated by air borne plant material. However that was the only time. Any other labs were not worried about it. I even repot plants in my present lab. The questions is " What would happen if plant material contaminates the samples? " Depending on what procedures are being done in that lab, if it doesn't skew the results, it's not a problem. Especially because the plants are improving air quality and work place productivity. The Inspectors might be more of a contamination problem than the plants ;-) Margaret From Sarah.Lewis <@t> nationwidechildrens.org Tue May 15 08:34:33 2012 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Tue May 15 08:34:40 2012 Subject: [Histonet] Schematic for trimming and Embedding Message-ID: <4BAB3E4AF573804EA9929EFD1E15817101AC82AC@L1PERDWXMB03.childrensroot.net> Does anyone have a schematic for trimming and embedding primate tissue that they can share with me? Thanks, Sarah Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Sarah.Lewis <@t> nationwidechildrens.org Tue May 15 08:50:08 2012 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Tue May 15 08:50:18 2012 Subject: [Histonet] HT, Job posting Columbus Ohio Message-ID: <4BAB3E4AF573804EA9929EFD1E15817101AC82CF@L1PERDWXMB03.childrensroot.net> Nationwide Children's Hospital Neuromuscular Technician Center for Gene Therapy Full time DAYS: Monday - Friday SHIFT: Dayshift Responsible for performance of complex nerve/muscle histology testing. Conducts histology technical skills for both clinical and research specimens. Assists in the daily functions of the Neuromuscular Laboratory. Visit http://www.nationwidechildrens.org/gd/applications/controller.cfm?&page=82 Keyword Histology for more information. Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From kc <@t> ka-recruiting.com Tue May 15 09:15:41 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Tue May 15 09:15:12 2012 Subject: [Histonet] Histotech jobs - Full time, permanent Message-ID: <884769822.1337091341718.JavaMail.cfservice@sl4app3> Good morning Histoneters, I hope you are well. I am a one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in advancing your career. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position in Louisville, KY. Exciting Histotech Job My client is a leading health care provider offering comprehensive anatomic and cytopathology services for health care providers throughout the South. Committed to quality, access, continuity and cost effectiveness of patient care their board-certified and registered clinical laboratory professionals provide the knowledge and expertise to assist clinicians in the management of patient care. They are looking to hire a full time Histotech to work out of their lab in Louisville. For consideration you must have an Associates or Bachelors degree in Histology (or equivalent) with at least 1 year of histology experience. HT or HTL (ASCP) certification is required. This Lab offers one of the better compensation & benefits package around including relocation assistance when necessary. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histotech/Cytotech Openings: * IN - Histotech * ME - Lead Surgical Pathologist * NC - Histology Supervisor 2nd shift * NC - Histology Manager * NC - Histotech 3rd shift (experienced) * NY - Western - Histotech - 1st shift * NY - NYC - Histotech * OH - Histotechnologist - 1st shift * NYC - Pathology Manager (commercial background) * FL - Treasure Coast - Histotech * FL - Cytotech - 1st shift * NV - Histotech, IHC To view additional opportunities please visit our website at www.ka-recruiting.com. I look forward to hearing from you. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From relia1 <@t> earthlink.net Tue May 15 10:46:04 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue May 15 10:46:13 2012 Subject: [Histonet] RELIA Solutions Hot Histology Jobs Alert!! Check out these exciting opportunities. Message-ID: <6EF9C28758904624AE8660915C1A573A@ownerf1abaad51> Hi Histonetters!! I hope you are having a great day. I want to take a minute and give you a heads up on some exciting positions that I am currently working on. All of my clients offer competitive salaries, excellent benefits a great group of people to work with and in most cases relocation assistance. Here is a partial list of positions: Histology Tech Nights - Louisville, KY Electron Microscopy Tech - Louisville, KY Molecular Tech - Nashville, TN Histology Tech All shifts - NY Histology Tech - AZ multiple openings ***If you are interested in any of these positions please contact me right away ***If you know anyone who might be interested in these positions have them contact me right away (we offer a 500 dollar referral fee for anyone you refer that we place)! *** If you are considering looking at a new opportunity and none of these are quite what you are looking for please contact me right away. (I have new positions coming in all of the time from all over the country. REMEMBER RELIA Solutions is the ONLY recruiting firm that specializes EXCLUSIVELY in the permanent placement of histology professionals. We offer 25+ years of experience helping people find their dream jobs!! Thanks- Pam relia1@earthlink.net 866-60RELIA 866-607-3542 Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mbmphoto <@t> gmail.com Tue May 15 20:25:43 2012 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue May 15 20:25:51 2012 Subject: [Histonet] Schematic for trimming and Embedding In-Reply-To: <4BAB3E4AF573804EA9929EFD1E15817101AC82AC@L1PERDWXMB03.childrensroot.net> References: <4BAB3E4AF573804EA9929EFD1E15817101AC82AC@L1PERDWXMB03.childrensroot.net> Message-ID: <6AD0ECC1-2AD2-415C-8EA7-A2E81752ECF3@gmail.com> Dear Sarah, Please can you be more specific. By schematic for trimming you mean pictures or written SOPs? Is it for primate tissue fixed or fresh frozen? If fixed, is the tissue for embedding in paraffin, or for cut free-floating? Just need more detail information, so I can help. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF On May 15, 2012, at 6:34 AM, Lewis, Sarah wrote: > Does anyone have a schematic for trimming and embedding primate tissue that they can share with me? > > Thanks, > Sarah > > Sarah E. Lewis HT, ASCP > The Research Institute at > Nationwide Children's Hospital > Center for Gene Therapy > Neuromuscular Division > Rm WA3110 > (614)722-2204 > > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Lewis <@t> nationwidechildrens.org Wed May 16 08:35:55 2012 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Wed May 16 08:36:11 2012 Subject: [Histonet] Schematic for trimming and Embedding In-Reply-To: <6AD0ECC1-2AD2-415C-8EA7-A2E81752ECF3@gmail.com> References: <4BAB3E4AF573804EA9929EFD1E15817101AC82AC@L1PERDWXMB03.childrensroot.net> <6AD0ECC1-2AD2-415C-8EA7-A2E81752ECF3@gmail.com> Message-ID: <4BAB3E4AF573804EA9929EFD1E15817101AC85BD@L1PERDWXMB03.childrensroot.net> Thank you Marie, This is fixed tissue for routine histology, paraffin embedding. I am looking for a picture schematic... I have one for rodent tissue but I can't seem to find anything for primate tissue. A written SOP would be very helpful as well if you wouldn't mind sharing. Thank you! Sarah -----Original Message----- From: Maria Mejia [mailto:mbmphoto@gmail.com] Sent: Tuesday, May 15, 2012 9:26 PM To: Lewis, Sarah Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Schematic for trimming and Embedding Dear Sarah, Please can you be more specific. By schematic for trimming you mean pictures or written SOPs? Is it for primate tissue fixed or fresh frozen? If fixed, is the tissue for embedding in paraffin, or for cut free-floating? Just need more detail information, so I can help. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF On May 15, 2012, at 6:34 AM, Lewis, Sarah wrote: > Does anyone have a schematic for trimming and embedding primate tissue that they can share with me? > > Thanks, > Sarah > > Sarah E. Lewis HT, ASCP > The Research Institute at > Nationwide Children's Hospital > Center for Gene Therapy > Neuromuscular Division > Rm WA3110 > (614)722-2204 > > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the sole > use of the intended recipient(s) and may contain confidential and > privileged information. The recipient is responsible to maintain the > confidentiality of this information and to use the information only > for authorized purposes. If you are not the intended recipient (or > authorized to receive information for the intended recipient), you are > hereby notified that any review, use, disclosure, distribution, > copying, printing, or action taken in reliance on the contents of this > e-mail is strictly prohibited. If you have received this communication > in error, please notify us immediately by reply e-mail and destroy all > copies of the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Wed May 16 09:36:44 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed May 16 09:37:05 2012 Subject: [Histonet] IHC Specialist Job in East Texas Message-ID: Hello, I have a full time permanent IHC Specialist Job opening in East Texas. If you are in East Texas or would be interested in relocating to East Texas please message me for more details. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.html Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From wilson6848 <@t> yahoo.com Wed May 16 12:00:26 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Wed May 16 12:00:32 2012 Subject: [Histonet] ANTIBODY VALIDATION PROCEDURE Message-ID: <1337187626.52079.YahooMailNeo@web120905.mail.ne1.yahoo.com> ? ?Hi, ???? How are you guys doing? I hope you are doing great. Please I will appreciate it, if you guys have a procedure on the ANTIBODY VALIDATION and would like to share it with me. ?? Thanks ? Wilson From LSebree <@t> uwhealth.org Wed May 16 12:26:53 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed May 16 12:27:15 2012 Subject: [Histonet] ANTIBODY VALIDATION PROCEDURE In-Reply-To: <1337187626.52079.YahooMailNeo@web120905.mail.ne1.yahoo.com> References: <1337187626.52079.YahooMailNeo@web120905.mail.ne1.yahoo.com> Message-ID: Follow the CAP guidelines for antibody validation; that's what we do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wilson A Sent: Wednesday, May 16, 2012 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANTIBODY VALIDATION PROCEDURE ? ?Hi, ???? How are you guys doing? I hope you are doing great. Please I will appreciate it, if you guys have a procedure on the ANTIBODY VALIDATION and would like to share it with me. ?? Thanks ? Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed May 16 13:29:30 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed May 16 13:29:37 2012 Subject: [Histonet] Old cryostat for parts Message-ID: <4FB3B9CA.7400.0077.1@harthosp.org> We have an "old" Miles "Tissue-Tek II" cryostat that is going to be discarded unless someone would like it for parts. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From mw <@t> personifysearch.com Wed May 16 14:19:06 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed May 16 14:19:15 2012 Subject: [Histonet] 6 New Histology Opportunities Message-ID: Good Afternoon Histonet, Personify currently has 6 exclusive histology openings throughout the US. We are searching for experienced histology professionals who are looking to join a global manufacturer of histology products. Below are the current searches we have available. *Field Applications Specialist ? Northeast* - Histology Consumables (Field based position, can live near a major airport.) *Field Applications Specialist ? Southeast* - Histology Consumables (Field based position, can live near a major airport.) *Field Applications Specialist ? Western Region* - Histology Consumables (Field based position, can live near a major airport, ideally in Southern CA.) *IHC Field Support ? NY Region* (NY,NJ). *QA Histologist *? Based in Northern IL, in house position. *In House Applications Specialist/Trainer* ? Based in Northern IL, in house position. To learn more about these opportunities please contact me at mw@personifysearch.com or call at 800.875.6188 ext. 103. Also, please connect with me on LinkedIn to receive updates on new positions. http://www.linkedin.com/in/mattwardpersonify Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From Courtney.Pierce <@t> quintiles.com Wed May 16 14:26:53 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Wed May 16 14:27:01 2012 Subject: [Histonet] CAP vs. CLIA Message-ID: Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From billodonnell <@t> catholichealth.net Wed May 16 14:29:43 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed May 16 14:29:53 2012 Subject: [Histonet] 6 New Histology Opportunities In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF93955B0BA9@chimsx08.CHI.catholichealth.net> Matt, would be interested in learning a bit more about the two positions listed below. Please contact me via my private e-mail bill@deaconbill.com Thanks - Bill O'Donnell Below are the current searches we have available. *Field Applications Specialist - Northeast* - Histology Consumables (Field based position, can live near a major airport.) *Field Applications Specialist - Southeast* - Histology Consumables (Field based position, can live near a major airport.) This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From dr <@t> personifysearch.com Wed May 16 14:51:20 2012 From: dr <@t> personifysearch.com (Danielle Robinson) Date: Wed May 16 14:51:28 2012 Subject: [Histonet] UK Sales Manager - Clinical/Medical (Danielle Robinson on behalf of Leica Microsystems) Message-ID: *Hi all,* *Our firm is globally retained with a world leading manufacturer in the histopathology arena. There are dozens of openings throughout the EU, so look for more opportunities to follow. This individual would manage a total of around 8 direct reports, so medical sales management experience is required. Detailed position description is below as well as a link to apply directly. Thanks!* Danielle Robinson Life Sciences Executive Recruiter International Access Phone: UK Toll Free: +44 20 30 51 42 69* *US Toll Free: (800) 875-6188 ext. 140 http://www.personifysearch.com/ http://www.linkedin.com/pub/danielle-robinson/23/5aa/70a * * * * *Sales Manager* *The Company:* Our client, Leica Biosystems, brings histopathology laboratories and researchers the highest quality, most comprehensive product range. With complete histology systems featuring innovative automation, reagents and consumables, Leica Biosystems offers the idea product for each histology step and high-productivity workflow solutions for the entire laboratory. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for a Sales Manager to be based in the UK. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Salary: Commensurate with Experience *Primary Responsibilities:* Set up direct sales strategy and achieve annual sales strategies and goals through management of Sales Representatives. Develop area sales plans to support Leica's customers. Provide the direction, leadership, coaching and mentoring for direct sales representatives. Advance the professional development, professional conduct, sales effectiveness and efficiency of staff. Additional Responsibilities: - Achieve and exceed Area's quarterly and annual sales quota, strategic and unit sales mix objectives, profit goal and market share goals - Develop annual sales quotas for sales representatives and dealers - Develop and train sales reps and specialists with clearly communicated sales and overall performance standards - Maintain strategy, support and pricing consistency between sales territories for National/Regional Accounts and National contracts - Coordinate the allocation and movement of Area's demonstration equipment with optimal efficiency and yield high inventory turns - Plan and coordinate semi-annual Area Sales Meetings; Attend and staff national or local sales meetings - Travel with sales reps and specialists providing individual coaching and professional development to members of the regional selling team - Complete Field Sales Management Reports and distribute report for each field visit - Ensure the completion of annual performance appraisals and merit increases for all direct reports *Education and Experience Required:* - BA/BSc in Life Science or equivalent; MBA/MSc in Life Science highly desired - 5+ years diagnostic sales experience - 3+ years sales management experience in capital equipment - Demonstrated ability to manage and develop sales reps and create teams - Histology/Cytology/Immunohistochemistry market knowledge is highly desired *Keywords:* Clinical Sales - Medical Sales - Diagnostics - Histology - Microscopes - Capital Equipment - Laboratory If you meet and/or exceed the experience criteria, please submit your resume by clicking on the link below. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered. Click here to apply online From dr <@t> personifysearch.com Wed May 16 14:59:54 2012 From: dr <@t> personifysearch.com (Danielle Robinson) Date: Wed May 16 15:00:05 2012 Subject: [Histonet] UK Based Field Support Scientist Positions (Danielle Robinson on behalf of Leica Microsystems) Message-ID: <12723b8cd3039ccf5bbe4e1ce1e65cfa@mail.gmail.com> Hi all, Two more UK based positions. These are technical sales support positions and there is one in South England for Core Histology and one that can be based anywhere within the UK for Advanced Staining (Immunohistochemistry). Full position descriptions below and direct apply links. Email me if you have any questions. Thanks! Danielle Robinson Life Sciences Executive Recruiter International Access Phone: UK Toll Free: +44 20 30 51 42 69* *US Toll Free: (800) 875-6188 ext. 140 http://www.personifysearch.com/ http://www.linkedin.com/pub/danielle-robinson/23/5aa/70a ------------------------------------------------------------------------------------------------------------------------------------------ *Field Support Scientist - Advanced Staining* *The Company:* Leica Biosystems brings histopathology laboratories and researchers the highest quality, most comprehensive product range. With complete histology systems featuring innovative automation, reagents and consumables, Leica Biosystems offers the idea product for each histology step and high-productivity workflow solutions for the entire laboratory. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* Leica currently has an opening for a Field Support Scientist to be based in any major city in the UK. Salary: Commensurate with experience *Primary Responsibilities:* This position will deliver first-class technical support, training, installations and demonstrations of Advanced Staining Instruments with promotion and sales of the Advanced training portfolio of reagents and consumables. The ultimate objective is to achieve customer satisfaction with Leica Biosystems Advanced Staining products in conjunction with growing Novocastra and core reagent product sales by marketing and sales initiatives. The FSS will provide installation, training and applications support to customers in their defined territory thus ensuring LBS Advanced Staining products have maximum "uptime" and therefore retains customers to generate reagent order intake. The FSS will work in close conjunction with the Sales Account Manager and FSS team to provide support with demonstrations, grow sales and margin and to increase market share though effective promotion and selling efforts. Additional Responsibilities: - Generate and develop sales and product awareness of LBS Advanced Staining product portfolio directly through customer visits and product information, samples and customer trials - Follow-up new leads generated by marketing activities, product presentations and workshops; In conjunction with the Advanced Staining Sales Account Manager, plan and implement sales initiatives in order to address specific tactical and/or strategic business needs; Record and actively manage activities in CRM - Research and identify potential with new customers in line with strategic directives in order to generate sales and to expand the LBS customer base; Identify and qualify opportunities to offer consumables reagents with instrumentation to further increase sales in new market segments - In collaboration with SAM colleagues, initiate and coordinate demos of Bond IHC stainer at customer sites; Lead customer visits for demonstration; Participate in congresses and meetings as necessary in order to promote existing and new antibodies and probes to customers in line with the existing promotional programs and strategies - Supply information on market share development, trends and market developments in geographic region of responsibility to Sales Management and Marketing - Performing customer training directly in the demonstration of instrument use, theranostics products, software functionality and IHC protocol development for the customer - Provide technical support to all existing customers as required including in-field troubleshooting of applications problems and diagnosing rudimentary hardware and software failures; In-office application support including special purpose IHC protocol development for specific customer requirements and "Helpline" technical customer support as required *Education and Experience Required:* 1 to 3 years Histology laboratory experience in clinical, research or industrial setting with a practical focus on IHC. 1-3 years experience in the optimization, operation, functionality and support of both manual and automated IHC instruments and related products is highly desired but not a requirement. Candidate must be self motivated, goal oriented and results driven with excellent interpersonal and communication skills. If you meet and/or exceed the experience criteria, please submit your resume by clicking on the "Inquire" link below. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered. Click here to apply online -------------------------------------------------------------------------------------------------------------------- *Field Support Scientist - Core Histology* *The Company:* Leica Biosystems brings histopathology laboratories and researchers the highest quality, most comprehensive product range. With complete histology systems featuring innovative automation, reagents and consumables, Leica Biosystems offers the idea product for each histology step and high-productivity workflow solutions for the entire laboratory. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* Leica currently has an opening for a Field Support Scientist to be based in Birmingham, London or Southern UK. Salary: Commensurate with experience *Primary Responsibilities:* This position will deliver first-class technical support, training, installations and demonstrations of Advanced Staining Instruments with promotion and sales of the Advanced training portfolio of reagents and consumables. The ultimate objective is to achieve customer satisfaction with Leica Biosystems Advanced Staining products in conjunction with growing Novocastra and core reagent product sales by marketing and sales initiatives. The FSS will provide installation, training and applications support to customers in their defined territory thus ensuring LBS Advanced Staining products have maximum "uptime" and therefore retains customers to generate reagent order intake. The FSS will work in close conjunction with the Sales Account Manager and FSS team to provide support with demonstrations, grow sales and margin and to increase market share though effective promotion and selling efforts. Additional Responsibilities: - Generate and develop sales and product awareness of LBS Advanced Staining product portfolio directly through customer visits and product information, samples and customer trials - Follow-up new leads generated by marketing activities, product presentations and workshops; In conjunction with the Advanced Staining Sales Account Manager, plan and implement sales initiatives in order to address specific tactical and/or strategic business needs; Record and actively manage activities in CRM - Research and identify potential with new customers in line with strategic directives in order to generate sales and to expand the LBS customer base; Identify and qualify opportunities to offer consumables reagents with instrumentation to further increase sales in new market segments - In collaboration with SAM colleagues, initiate and coordinate demos of Bond IHC stainer at customer sites; Lead customer visits for demonstration; Participate in congresses and meetings as necessary in order to promote existing and new antibodies and probes to customers in line with the existing promotional programs and strategies - Supply information on market share development, trends and market developments in geographic region of responsibility to Sales Management and Marketing - Performing customer training directly in the demonstration of instrument use, theranostics products, software functionality and IHC protocol development for the customer - Provide technical support to all existing customers as required including in-field troubleshooting of applications problems and diagnosing rudimentary hardware and software failures; In-office application support including special purpose IHC protocol development for specific customer requirements and "Helpline" technical customer support as required *Education and Experience Required:* 1 to 3 years Histology laboratory experience in clinical, research or industrial setting with a practical focus on IHC. 1-3 years experience in the optimization, operation, functionality and support of both manual and automated IHC instruments and related products is highly desired but not a requirement. Candidate must be self motivated, goal oriented and results driven with excellent interpersonal and communication skills. If you meet and/or exceed the experience criteria, please submit your resume by clicking on the link below. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered. Click here to apply online From wilson6848 <@t> yahoo.com Wed May 16 21:32:04 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Wed May 16 21:32:07 2012 Subject: [Histonet] ANTIBODY VALIDATION PROCEDURE Message-ID: <1337221924.83207.YahooMailNeo@web120902.mail.ne1.yahoo.com> ?Hi, How are you guys doing? I hope you are doing great. Please I will appreciate it, if you guys have a procedure on the ANTIBODY VALIDATION and would like to share it with me. Thanks Wilson From JMacDonald <@t> mtsac.edu Wed May 16 22:08:19 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed May 16 22:09:14 2012 Subject: [Histonet] need a speaker Message-ID: Hi All, The Mt. San Antonio College Caduceus Club Histotech Section is having a meeting on Monday, May 21 at 4:30 PM. We have been unable to secure a speaker for this meeting. Is there anyone in the area that would like to speak to the students about H&E Staining or fixation? They are a great group of enthusiastic students who would like to hear from someone other than me. :) Thank you, Jennifer MacDonald jmacdonald@mtsac.edu 909.274-4884 From ibernard <@t> uab.edu Thu May 17 00:38:33 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu May 17 00:38:41 2012 Subject: [Histonet] Cytology in Histo Lab Message-ID: Our histopathology lab does cytology processing of Non-Gyns samples. Looking for a good cytology spray fixative to fix nipple discharge slides or smears. Need name of product, catalog number and distributor of this product. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From micropathlabs <@t> yahoo.com Thu May 17 06:22:19 2012 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu May 17 06:22:23 2012 Subject: [Histonet] Cytology Staining Message-ID: <1337253739.33117.YahooMailNeo@web161705.mail.bf1.yahoo.com> Hello all. During an inspection yesterday, the inspector made a brief, vague reference to?cytology staining now being deemed "high complexity". Does anyone have information and/or a reference for this? I'd appreciate any information anyone could provide. Thanks a bunch. ? Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From HornHV <@t> archildrens.org Thu May 17 07:26:15 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 17 07:26:24 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From micropathlabs <@t> yahoo.com Thu May 17 07:56:17 2012 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu May 17 07:56:21 2012 Subject: [Histonet] Cytology Staining In-Reply-To: <1337253739.33117.YahooMailNeo@web161705.mail.bf1.yahoo.com> References: <1337253739.33117.YahooMailNeo@web161705.mail.bf1.yahoo.com> Message-ID: <1337259377.60692.YahooMailNeo@web161706.mail.bf1.yahoo.com> This inspector specifically referred to the pap stain (sorry I didn't make this clear initially). We briefly chatted about what the difference would be between the H&E and the pap stains as well as automation of both stains. The inspector didn't seem to have more insight so I thought I'd ask the real experts...you all. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From: Sheila Haas To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, May 17, 2012 7:22 AM Subject: [Histonet] Cytology Staining Hello all. During an inspection yesterday, the inspector made a brief, vague reference to?cytology staining now being deemed "high complexity". Does anyone have information and/or a reference for this? I'd appreciate any information anyone could provide. Thanks a bunch. ? Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Thu May 17 08:21:35 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu May 17 08:21:10 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> Message-ID: Thank you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 8:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From mpence <@t> grhs.net Thu May 17 08:26:32 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Thu May 17 08:26:38 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E28@is-e2k3.grhs.net> This is a great info source to have. Thanks, Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Thursday, May 17, 2012 8:22 AM To: Horn, Hazel V; Courtney Pierce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: CAP vs. CLIA Thank you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 8:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 17 08:27:26 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 17 08:27:32 2012 Subject: [Histonet] Cytology Staining In-Reply-To: <1337259377.60692.YahooMailNeo@web161706.mail.bf1.yahoo.com> Message-ID: <1337261246.97048.YahooMailClassic@web162106.mail.bf1.yahoo.com> You are right in many ways. Pap stain (now in its 62nd birthday!) is a routine stain that many labs do automated. It seems that your inspector needed to "say something" as if "saying something" means "knowing more than you". I know of no regulation that considers Papa stain as a complex task. Ren? J. --- On Thu, 5/17/12, Sheila Haas wrote: From: Sheila Haas Subject: Re: [Histonet] Cytology Staining To: "Sheila Haas" , "histonet@lists.utsouthwestern.edu" Date: Thursday, May 17, 2012, 8:56 AM This inspector specifically referred to the pap stain (sorry I didn't make this clear initially). We briefly chatted about what the difference would be between the H&E and the pap stains as well as automation of both stains. The inspector didn't seem to have more insight so I thought I'd ask the real experts...you all. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From: Sheila Haas To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, May 17, 2012 7:22 AM Subject: [Histonet] Cytology Staining Hello all. During an inspection yesterday, the inspector made a brief, vague reference to?cytology staining now being deemed "high complexity". Does anyone have information and/or a reference for this? I'd appreciate any information anyone could provide. Thanks a bunch. ? Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Thu May 17 08:41:51 2012 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu May 17 08:42:00 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> Message-ID: <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From HornHV <@t> archildrens.org Thu May 17 09:05:09 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 17 09:05:21 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From micropathlabs <@t> yahoo.com Thu May 17 09:11:59 2012 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu May 17 09:12:08 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> Message-ID: <1337263919.96011.YahooMailNeo@web161705.mail.bf1.yahoo.com> I received the same alert and interpreted it the same as Hazel did. Also,?the state inspector in my facility yesterday had the same interpretation. ? Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From: "Horn, Hazel V" To: "'Willis, Donna G.'" ; 'Courtney Pierce' Cc: "histonet@lists.utsouthwestern.edu" Sent: Thursday, May 17, 2012 10:05 AM Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation.? The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results."? To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person.? Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me.? In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster?? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital **********************? IMPORTANT--PLEASE READ? ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information.? If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited.? If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Thu May 17 09:23:41 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu May 17 09:23:52 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> Message-ID: I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From JMacDonald <@t> mtsac.edu Thu May 17 09:23:58 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu May 17 09:24:58 2012 Subject: [Histonet] Mt. San Antonio College - Need speaker Message-ID: I guess it would help to know where Mt. San Antonio College is. We are located in Southern California, about 35 miles east of Los Angeles. 1100 N. Grand Ave. Walnut, CA 91789 From Donna.Willis <@t> baylorhealth.edu Thu May 17 09:25:39 2012 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu May 17 09:25:48 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> Message-ID: <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt> Very well said Jesus. I agree. Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Thursday, May 17, 2012 9:24 AM To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From lblazek <@t> digestivespecialists.com Thu May 17 09:26:59 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu May 17 09:27:22 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E3913856F3C99@IBMB7Exchange.digestivespecialists.com> You hit the nail on the head! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, May 17, 2012 10:24 AM To: 'Horn, Hazel V'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abdulrahmanmsaadalla <@t> gmail.com Thu May 17 09:49:12 2012 From: abdulrahmanmsaadalla <@t> gmail.com (abdulrahman saadalla) Date: Thu May 17 09:49:41 2012 Subject: [Histonet] (no subject) Message-ID: Dear all I am trying to do IHC double stain for GITR and Foxp3. I would be grateful if you provide me any advices and tips. Regard, Abdulrahman From dgoodwin <@t> rwjuhh.edu Thu May 17 10:31:38 2012 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Thu May 17 10:31:51 2012 Subject: [Histonet] NJSH-Thermo Scientific One-Day Seminar in Philadelphia Message-ID: <09411E0112A96A459D8D5FBDAB9C15C725299A68A3@HAMEXMBA.rwjham.local> Hi everyone, I am excited to announce that the New Jersey Society for Histotechnology has partnered with Thermo- Scientific to present a one-day seminar as part of the Thermo Scientific Educational Series. Date: Saturday June 2, 2012 Location: Holiday Inn Philadelphia Stadium Please see use the link below (or copy and paste it into your browser's address line) to access the NJSH web site for information regarding the program, hotel accommodations, and to register. Hope to see you there! Pedro Louro President - NJSH http://www.njsh.org/njsh/Meetings.html From cbrya <@t> lexclin.com Thu May 17 10:41:29 2012 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu May 17 10:41:37 2012 Subject: [Histonet] Ventana/ LIS Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF41740541A7@EXCHANGESB> Can the Ventana Ultra system be interfaced to your LIS so that personnel do not have to re-enter the case # and patient ID in the Ultra? I know that Ventana offers the Vantage system that connects everything, but can one merely interface their LIS directly to the Ultra? Anyone have any ideas or suggestions? Thank you, Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From HornHV <@t> archildrens.org Thu May 17 11:17:09 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 17 11:17:20 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3913856F3C99@IBMB7Exchange.digestivespecialists.com> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <5A2BD13465E061429D6455C8D6B40E3913856F3C99@IBMB7Exchange.digestivespecialists.com> Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A1605@EVS1.archildrens.org> I certainly agree with you Jesus. I felt like my face had been slapped. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Thursday, May 17, 2012 9:27 AM To: 'Jesus Ellin'; Horn, Hazel V; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA You hit the nail on the head! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, May 17, 2012 10:24 AM To: 'Horn, Hazel V'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From joelleweaver <@t> hotmail.com Thu May 17 11:24:52 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 17 11:25:04 2012 Subject: [Histonet] Ventana/ LIS In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF41740541A7@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF41740541A7@EXCHANGESB> Message-ID: My understanding is that they want you to buy the Vantage system. I would consider them a "closed system", meaning that they set it up for little manipulation by users- that is a plus or minus depending on your viewpoint. It appeals to labs who don't want to do a lot of training, and from their mouths "they make it so "anyone" can use it". I would say that it does ensure a certain amount of reliability and standardization, but that is not really my stance on good use of instrumentation-but another topic entirely! I would say that in my dealings with Ventana their are protective of their products /design so that it is difficult to stray from the system( they want you to buy their stuff)- not to say it can't be done. That is not really a criticism of them, and makes good business sense, just a plus or minus to be considered when you buy instruments. It may be possible with middleware of some sort or with customized programming to do what you wish to do. They would probably have to allow access or provide a map to the code. If you have programmers in your LIS department you might check with them and probably depends on what computer system you have ( older or object oriented perhaps). I think that situation may change with EMR and competitors that can directly interface, as this will be an issue of increasing importance in my opinion. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: cbrya@lexclin.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 17 May 2012 11:41:29 -0400 > Subject: [Histonet] Ventana/ LIS > > Can the Ventana Ultra system be interfaced to your LIS so that personnel do not have to re-enter the case # and patient ID in the Ultra? I know that Ventana offers the Vantage system that connects everything, but can one merely interface their LIS directly to the Ultra? > Anyone have any ideas or suggestions? > > Thank you, > Carol > > Carol Bryant, CT (ASCP) > Cytology/Histology Manager > Lexington Clinic > Phone (859) 258-4082 > Fax (859) 258-4081 > cbrya@lexclin.com > > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu May 17 11:40:38 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 17 11:40:36 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A1605@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <5A2BD13465E061429D6455C8D6B40E3913856F3C99@IBMB7Exchange.digestivespecialists.com> <25A4DE08332B19499904459F00AAACB719BB4A1605@EVS1.archildrens.org> Message-ID: <8D7C2D242DBD45498006B21122072BF8B4F4E528@MCINFRWEM003.ucsfmedicalcenter.org> " CAP does not consider us testing personnel. How they come by this is a mystery to me. " The "test" is the interpretation of results - providing diagnostic decisions on stains. Histology staff perform the stains - apply reagents to a slide following a pre-determined protocol. The pathologist, or someone he delegates it to, does the interpretation. In the clinical lab the techs actually validate results. Most of these are machine-produced numbers so the tech is validating that the machine works correctly. But since they are "signing out" the result they are considered as doing the testing. Cytotechs interpret slides and produce an independent result that they sign off and report out. They are doing high complexity testing. A clin lab tech who reads micro slides, plates or whatever and provides a result that they alone sign off on before sending out the result are doing high complexity testing. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 9:17 AM To: 'Blazek, Linda'; 'Jesus Ellin'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA I certainly agree with you Jesus. I felt like my face had been slapped. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Thursday, May 17, 2012 9:27 AM To: 'Jesus Ellin'; Horn, Hazel V; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA You hit the nail on the head! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, May 17, 2012 10:24 AM To: 'Horn, Hazel V'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu May 17 11:48:40 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 17 11:48:40 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF8B4F4E540@MCINFRWEM003.ucsfmedicalcenter.org> Here is the CLIA method of determining a high complexity test. Taken from : http://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm124208.htm Tim Morken ******************************************************** CLIA Categorization Criteria Each specific laboratory test system, assay, and examination is graded for level of complexity by assigning scores of 1, 2, or 3 for each of the seven criteria listed below. A score of 1 indicates the lowest level of complexity, and the score of 3 indicates the highest level. These scores are totaled. Test systems, assays or examinations receiving scores of 12 or less are categorized as moderate complexity, while those receiving scores above 12 are categorized as high complexity. Note: A score of 2 will be assigned to a criteria heading when the characteristics for a particular test are intermediate between the descriptions listed for scores of 1 and 3 Tests may also be categorized as waived.1 Criteria for Categorization (1) Knowledge. Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction. Score 3. Specialized scientific and technical knowledge is essential to perform preanalytic, analytic or postanalytic phases of the testing. (2) Training and experience. Score 1. (A) Minimal training is required for preanalytic, analytic and postanalytic phases of the testing process; and (B) Limited experience is required to perform the test. Score 3. (A) Specialized training is essential to perform the preanalytic, analytic or postanalytic testing process; or Substantial experience may be necessary for analytic test performance. (3) Reagents and materials preparation. Score 1. (A) Reagents and materials are generally stable and reliable; and (B) Reagents and materials are prepackaged, or premeasured, or require no special handling, precautions or storage conditions. Score 3. (A) Reagents and materials may be labile and may require special handling to assure reliability; or (B) Reagents and materials preparation may include manual steps such as gravimetric or volumetric measurements. (4) Characteristics of operational steps. Score 1. Operational steps are either automatically executed (such as pipetting, temperature monitoring, or timing of steps), or are easily controlled. Score 3. Operational steps in the testing process require close monitoring or control, and may require special specimen preparation,precise temperature control or timing of procedural steps, accuratepipetting, or extensive calculations. (5) Calibration, quality control, and proficiency testing materials. Score 1. (A) Calibration materials are stable and readily available; (B) Quality control materials are stable and readily available; and (C) External proficiency testing materials, when available, are stable. Score 3. (A) Calibration materials, if available, may be labile; (B) Quality control materials may be labile, or not available; or (C) External proficiency testing materials, if available, may be labile. (6) Test system troubleshooting and equipment maintenance. Score 1. (A) Test system troubleshooting is automatic or self-correcting, or clearly described or requires minimal judgment; and (B) Equipment maintenance is provided by the manufacturer, is seldom needed, or can easily be performed. Score 3. (A) Troubleshooting is not automatic and requires decision-making and direct intervention to resolve most problems; or (B) Maintenance requires special knowledge, skills, and abilities. (7) Interpretation and judgment. Score 1. (A) Minimal interpretation and judgment are required to perform preanalytic, analytic and postanalytic processes; and (B) Resolution of problems requires limited independent interpretation and judgment; and Score 3. (A) Extensive independent interpretation and judgment are required to perform the preanalytic, analytic or postanalytic processes; and (B) Resolution of problems requires extensive interpretation and judgment. ******************************************** -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu May 17 11:50:29 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 17 11:50:30 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B4F4E528@MCINFRWEM003.ucsfmedicalcenter.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <5A2BD13465E061429D6455C8D6B40E3913856F3C99@IBMB7Exchange.digestivespecialists.com> <25A4DE08332B19499904459F00AAACB719BB4A1605@EVS1.archildrens.org> <8D7C2D242DBD45498006B21122072BF8B4F4E528@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <8D7C2D242DBD45498006B21122072BF8B4F4E54B@MCINFRWEM003.ucsfmedicalcenter.org> I should have included grossing. Grossing is high complexity because the person has to use independent judgment when examining and sampling the specimen. They are essentially providing a result of a procedure to be used to get more results down stream. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, May 17, 2012 9:41 AM To: Horn, Hazel V; 'Blazek, Linda'; 'Jesus Ellin'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA " CAP does not consider us testing personnel. How they come by this is a mystery to me. " The "test" is the interpretation of results - providing diagnostic decisions on stains. Histology staff perform the stains - apply reagents to a slide following a pre-determined protocol. The pathologist, or someone he delegates it to, does the interpretation. In the clinical lab the techs actually validate results. Most of these are machine-produced numbers so the tech is validating that the machine works correctly. But since they are "signing out" the result they are considered as doing the testing. Cytotechs interpret slides and produce an independent result that they sign off and report out. They are doing high complexity testing. A clin lab tech who reads micro slides, plates or whatever and provides a result that they alone sign off on before sending out the result are doing high complexity testing. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 9:17 AM To: 'Blazek, Linda'; 'Jesus Ellin'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA I certainly agree with you Jesus. I felt like my face had been slapped. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] Sent: Thursday, May 17, 2012 9:27 AM To: 'Jesus Ellin'; Horn, Hazel V; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA You hit the nail on the head! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, May 17, 2012 10:24 AM To: 'Horn, Hazel V'; 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. 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Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. 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Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MYang <@t> emc.org Thu May 17 11:59:10 2012 From: MYang <@t> emc.org (Yang, Mari) Date: Thu May 17 11:59:24 2012 Subject: [Histonet] Cytology Staining In-Reply-To: <1337253739.33117.YahooMailNeo@web161705.mail.bf1.yahoo.com> References: <1337253739.33117.YahooMailNeo@web161705.mail.bf1.yahoo.com> Message-ID: <3340FC2AE9CFEE4E9D001D077700C6A2253ED944@NT106.info.sys> Sheila, Maybe the inspector was referring to the prep? Many cytology labs use automated staining. If you find out any information, I'd love to know. Thanks, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, May 17, 2012 4:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining Hello all. During an inspection yesterday, the inspector made a brief, vague reference to?cytology staining now being deemed "high complexity". Does anyone have information and/or a reference for this? I'd appreciate any information anyone could provide. Thanks a bunch. ? Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu May 17 12:35:08 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 17 12:35:19 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B4F4E54B@MCINFRWEM003.ucsfmedicalcenter.org> References: , <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org>, <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt>, <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org>, , <5A2BD13465E061429D6455C8D6B40E3913856F3C99@IBMB7Exchange.digestivespecialists.com>, <25A4DE08332B19499904459F00AAACB719BB4A1605@EVS1.archildrens.org>, <8D7C2D242DBD45498006B21122072BF8B4F4E528@MCINFRWEM003.ucsfmedicalcenter.org>, <8D7C2D242DBD45498006B21122072BF8B4F4E54B@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: TimI agree with your comments and the interpretation of the regulations( and these are regulations not value judgments). I still think however that CLIA should be updated to include all activities, in all potential settings, that well trained histology personnel may perform. I can think of several histology jobs where output was produced that stood on its own, but did get fed into later processes for clinical interpretation and decision-making. I just think the picture/definition is rather narrow. I think that if you use the testing phases, our just crosses over some but not all phases( preanalytic & analytic), whereas other testing has post-analytic. Maybe the word should be oversight? Histology techs sometimes just get offended because emotionally, this feels like it is implied that we don't know or understand our activities or supply technical correction. I think there is similarity to grossing in application, and likewise, histology produces a result that moves to later in the process stream. Though admittedly there is wide variation of skill and experience and different types of roles out there, I do think most of us monitor and "validate" the technical quality of our output, and are very concerned about the patient and the impact of our technical performance has on their outcomes... Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: Timothy.Morken@ucsfmedctr.org; HornHV@archildrens.org; lblazek@digestivespecialists.com; JEllin@yumaregional.org; Donna.Willis@baylorhealth.edu; Courtney.Pierce@quintiles.com > Date: Thu, 17 May 2012 09:50:29 -0700 > CC: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > I should have included grossing. Grossing is high complexity because the person has to use independent judgment when examining and sampling the specimen. They are essentially providing a result of a procedure to be used to get more results down stream. > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, May 17, 2012 9:41 AM > To: Horn, Hazel V; 'Blazek, Linda'; 'Jesus Ellin'; 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > " CAP does not consider us testing personnel. How they come by this is a mystery to me. " > > The "test" is the interpretation of results - providing diagnostic decisions on stains. Histology staff perform the stains - apply reagents to a slide following a pre-determined protocol. The pathologist, or someone he delegates it to, does the interpretation. > > In the clinical lab the techs actually validate results. Most of these are machine-produced numbers so the tech is validating that the machine works correctly. But since they are "signing out" the result they are considered as doing the testing. > > Cytotechs interpret slides and produce an independent result that they sign off and report out. They are doing high complexity testing. > > A clin lab tech who reads micro slides, plates or whatever and provides a result that they alone sign off on before sending out the result are doing high complexity testing. > > > Tim Morken > Department of Pathology > UC San Francisco Medical Center > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 9:17 AM > To: 'Blazek, Linda'; 'Jesus Ellin'; 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > I certainly agree with you Jesus. I felt like my face had been slapped. > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > > -----Original Message----- > From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] > Sent: Thursday, May 17, 2012 9:27 AM > To: 'Jesus Ellin'; Horn, Hazel V; 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: CAP vs. CLIA > > You hit the nail on the head! > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin > Sent: Thursday, May 17, 2012 10:24 AM > To: 'Horn, Hazel V'; 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. > > Jesus Ellin HT/PA ASCP, BSBE,MSBE > Yuma Regional Medical Center > Anatomic Pathology Supervisor > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 7:05 AM > To: 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > It was a CAP e alert dated April 2, 2012 > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > > -----Original Message----- > From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] > Sent: Thursday, May 17, 2012 8:42 AM > To: Horn, Hazel V; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: CAP vs. CLIA > > I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. > > This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Histology Lab Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 7:26 AM > To: 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: > > " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? > > As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." > > And goes on to say: > "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? > > Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce > Sent: Wednesday, May 16, 2012 2:27 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP vs. CLIA > > > Can someone help me with the High Complexity Test with CAP vs. CLIA. > Thanks > Courtney Pierce > IHC Specialist > Quintiles > Translational R&D - Oncology > Innovation > Navigating the new health > > 610 Oakmont Lane > Westmont, IL 60559 > > Office: + 630-203-6234 > courtney.pierce@quintiles.com > > clinical | commercial | consulting | capital > > > ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. > ************************************************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu May 17 12:46:05 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 17 12:46:01 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt> Message-ID: <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org> Jesus wrote: " I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. " Certainly the regulations limit the "high complexity" designation to interpretation of procedure results, but that does not mean a facility does not need very highly trained and competent technologists to do the protocols that lead to good interpretation. It simply highlights the difference between running slides through protocols vs looking at the result and determining a diagnosis. I'm sure most here will see the difference. Remember that CAP is a simply a deemed agency of CLIA - that is, CMS (Centers for Medicare and Medicaid, which administers the CLIA regulations) delegates to CAP (and Joint Commission) the authority to accredit laboratories. CAP cannot make up new regulations, only enforce existing CLIA regulations. However, the CLIA regulations are by necessity very general so they can apply to any kind of laboratory operations, current or future. CAP has the leeway to look at what labs are doing and determine if the CLIA regulations apply to those tasks. However, CAP must submit their proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are implemented. CAP checklists are far more complex than they were 20 years ago. But the histo lab is far more complex as well, and regulators (as well as the public) are looking much more closely at histology because of some major mistakes that have happened largely due to lack of rigor in testing validation and implementation. A lot of that has to do with small labs doing complex testing (interpretation) with methods they were/are not fully competent to do primarily due to lack of experience and expertise. While the accreditation process is getting more onerous, it is also forcing labs to be much more professional in their operations - always a good thing, I think. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, May 17, 2012 7:26 AM To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA Very well said Jesus. I agree. Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Thursday, May 17, 2012 9:24 AM To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abdulrahmanmsaadalla <@t> gmail.com Thu May 17 12:47:04 2012 From: abdulrahmanmsaadalla <@t> gmail.com (abdulrahman saadalla) Date: Thu May 17 12:47:08 2012 Subject: [Histonet] (no subject) Message-ID: Dear all I am do IHC double staining for CD25 and Foxp3. I would be grateful if you provide with your tips and advices for perfect results. Regerd, Abdulrahman. From JEllin <@t> yumaregional.org Thu May 17 12:52:44 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu May 17 12:52:52 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org> References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt> <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: I completely agree with you on this. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, May 17, 2012 10:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA Jesus wrote: " I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. " Certainly the regulations limit the "high complexity" designation to interpretation of procedure results, but that does not mean a facility does not need very highly trained and competent technologists to do the protocols that lead to good interpretation. It simply highlights the difference between running slides through protocols vs looking at the result and determining a diagnosis. I'm sure most here will see the difference. Remember that CAP is a simply a deemed agency of CLIA - that is, CMS (Centers for Medicare and Medicaid, which administers the CLIA regulations) delegates to CAP (and Joint Commission) the authority to accredit laboratories. CAP cannot make up new regulations, only enforce existing CLIA regulations. However, the CLIA regulations are by necessity very general so they can apply to any kind of laboratory operations, current or future. CAP has the leeway to look at what labs are doing and determine if the CLIA regulations apply to those tasks. However, CAP must submit their proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are implemented. CAP checklists are far more complex than they were 20 years ago. But the histo lab is far more complex as well, and regulators (as well as the public) are looking much more closely at histology because of some major mistakes that have happened largely due to lack of rigor in testing validation and implementation. A lot of that has to do with small labs doing complex testing (interpretation) with methods they were/are not fully competent to do primarily due to lack of experience and expertise. While the accreditation process is getting more onerous, it is also forcing labs to be much more professional in their operations - always a good thing, I think. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, May 17, 2012 7:26 AM To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA Very well said Jesus. I agree. Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Thursday, May 17, 2012 9:24 AM To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. Jesus Ellin HT/PA ASCP, BSBE,MSBE Yuma Regional Medical Center Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:05 AM To: 'Willis, Donna G.'; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA It was a CAP e alert dated April 2, 2012 Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] Sent: Thursday, May 17, 2012 8:42 AM To: Horn, Hazel V; 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: RE: CAP vs. CLIA I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, May 17, 2012 7:26 AM To: 'Courtney Pierce' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP vs. CLIA CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." And goes on to say: "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce Sent: Wednesday, May 16, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP vs. CLIA Can someone help me with the High Complexity Test with CAP vs. CLIA. Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From kc <@t> ka-recruiting.com Thu May 17 21:46:33 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Thu May 17 21:46:42 2012 Subject: [Histonet] Histotech job in Las Vegas Message-ID: <2045247280.1337309193429.JavaMail.cfservice@sl4app2> Job Description: * Associates degree required, Bachelor?s degree preferred * Certified as a histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required * 1+ years of experience preferred * Good customer service * Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. Job Description: * Associates degree required, Bachelor?s degree preferred * Certified as a histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required * 1+ years of experience preferred * Good customer service * Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. Hi histonet community, I hope you are well. I am a one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in advancing your career. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position in Las Vegas, NV Exciting Histotech Job Job Description: * Associates degree required, Bachelor?s degree preferred * Certified as a histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required * 1+ years of experience preferred * Good customer service * Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. Job Description: * Associates degree required, Bachelor?s degree preferred * Certified as a histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required * 1+ years of experience preferred * Good customer service * Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. Job Description: * Associates degree required, Bachelor?s degree preferred * Certified as a histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required * 1+ years of experience preferred * Good customer service * Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. Job Description: * Associates degree required, Bachelor?s degree preferred * Certified as a histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required * 1+ years of experience preferred * Good customer service * Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. Associates degree required, Bachelor?s degree preferred. Certified as a Histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology required . 1+ years of experience preferred Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. This Lab offers one of the better compensation packages around including relocation assistance when necessary. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histotech/Cytotech Openings: * IN - Histotech * ME - Lead Surgical Pathologist * NC - Histology Supervisor 2nd shift * NC - Histology Manager * NC - Histotech 3rd shift (experienced) * NY - Western - Histotech - 1st shift * NY - NYC - Histotech * OH - Histotechnologist - 1st shift * NYC - Pathology Manager (commercial background) * FL - Treasure Coast - Histotech * FL - Cytotech - 1st shift * NV - Histotech, IHC To view additional opportunities please visit our website at www.ka-recruiting.com. I look forward to hearing from you. Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From kc <@t> ka-recruiting.com Fri May 18 07:37:32 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Fri May 18 12:28:56 2012 Subject: [Histonet] Histology job in Las vegas Message-ID: <734354233.1337344651908.JavaMail.cfservice@SL4APP4> Good morning Histonet Community, I hope you are well. I am a one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in advancing your career. We are completely free of charge to candidates and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position in Las Vegas, NV Exciting Histotech Job This position requires an Associates or Bachelor?s degree in Histology. Must be certified as a Histotechnician or Histotechnologist (HT/HTL) by the American Society of Clinical Pathology. A minimum of 1 year of experience is preferred. Must be a US citizen or eligible to work in the US for any employer. No sponsorship will be provided. This Lab offers one of the better compensation packages around including relocation assistance when necessary. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histotech/Cytotech Openings: * IN - Histotech * ME - Lead Surgical Pathologist * NC - Histology Supervisor 2nd shift * NC - Histology Manager * NC - Histotech 3rd shift (experienced) * KY ? Histotech ? 3rd shift * NY - Western - Histotech - 1st shift * NY - NYC - Histotech * OH - Histotechnologist - 1st shift * NYC - Pathology Manager (commercial background) * FL - Treasure Coast - Histotech * NV - Histotech, IHC * FL - Cytotech - 1st shift To view additional opportunities please visit our website at www.ka-recruiting.com . I look forward to hearing from you. Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From Vickroy.Jim <@t> mhsil.com Fri May 18 09:05:50 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri May 18 13:00:02 2012 Subject: [Histonet] Elastic Stain Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF53A@mmc-mail.ad.mhsil.com> I am looking for an Elastic/H&E stain procedure for lung tissue. Please submit procedures to Jim Vickroy. vickroy.jim@mhsil.com We sent this last week and because of the wonderful world of IT systems, my tech could not open the responses she got. We apologize if you had already sent a response to Kathy Argenta. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Vickroy.Jim <@t> mhsil.com Fri May 18 12:00:09 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri May 18 13:32:08 2012 Subject: [Histonet] Cognitive Printers from General Data Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF542@mmc-mail.ad.mhsil.com> Does anybody have any experience with trouble shooting barcoded slide label quality from these printers? We are cleaning the heads twice a week and still experience uneven printing regularly. General Data usually say problems are due to "dirty" printer heads. We know there are some adjustments that can be made to the printers themselves but just wondered if anyone has experienced the same kind of problems and how they resolved them. Any help would be appreciated. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Ken_Marissael <@t> vwr.com Fri May 18 13:03:06 2012 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Fri May 18 13:40:53 2012 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 05/18/2012 and will not return until 05/22/2012. I will be away until Tuesday and will not have access to e-mail or the internet. Please contact contact customer service at 800-932-5000. Press 6 for Healthcare. From CloughleyN <@t> rvh.on.ca Fri May 18 15:02:35 2012 From: CloughleyN <@t> rvh.on.ca (Cloughley-Gray, Nancy) Date: Fri May 18 15:00:22 2012 Subject: [Histonet] Help Message-ID: <4E09C2A3596693458A4BDDD2245F3146138A30@RVH-EXMB2.rvh.on.ca> I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of the normal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT From rjbuesa <@t> yahoo.com Fri May 18 15:27:04 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 18 15:27:06 2012 Subject: [Histonet] Help In-Reply-To: <4E09C2A3596693458A4BDDD2245F3146138A30@RVH-EXMB2.rvh.on.ca> Message-ID: <1337372824.23092.YahooMailClassic@web162106.mail.bf1.yahoo.com> As to your issue of tissue not adhering to the slides, you could try to check the expiration date of your (+) slides. Perhaps it is just an issue?with the slide. As to controlling the "concentration" of an antibody by changing the incubation time, that is somewhat unorthodox to say the least. You modify a concentration with dilution, not with time. Perhaps you could modify the HIER step, or try to dilute the antibody. Ren? J. --- On Fri, 5/18/12, Cloughley-Gray, Nancy wrote: From: Cloughley-Gray, Nancy Subject: [Histonet] Help To: "'histonet@lists.utsouthwestern.edu'" Cc: "Callan, Lisa" Date: Friday, May 18, 2012, 4:02 PM I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of the normal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Sat May 19 12:15:45 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Sat May 19 12:16:57 2012 Subject: [Histonet] Ventana/ LIS In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF41740541A7@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF41740541A7@EXCHANGESB> Message-ID: <004e01cd35e3$1384efe0$3a8ecfa0$@pathview.com> Good afternoon Carol, I represent an LIS vendor and I can definitely tell you that 'technically', your LIS can be directly interfaced to the Ultra because we have done that ourselves. On the other hand, it's an entirely different issue as to whether Ventana will allow you to do this. Now that they have their Vantage offering, I believe that they are trying to run all their interfaces through this product. I'm not 100% certain of that fact though. I hope it's not true as it turns a pretty straightforward, HL7 based interface with a fairly standard cost into a significantly larger project both in terms of cost and implementation. If you'd like to discuss any of the technical details, please feel free to contact me directly. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, May 17, 2012 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana/ LIS Can the Ventana Ultra system be interfaced to your LIS so that personnel do not have to re-enter the case # and patient ID in the Ultra? I know that Ventana offers the Vantage system that connects everything, but can one merely interface their LIS directly to the Ultra? Anyone have any ideas or suggestions? Thank you, Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Sun May 20 22:37:56 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Sun May 20 22:38:05 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: , <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org>, <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt>, <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org>, , <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt>, <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org>, Message-ID: I seemed to have missed something or it might have been all the fresh sea air I got in Tampa at the FSH, but I do not understand the outrage expressed towards CLIA and CAP because we are not listed as testing personnel. I applaud everyone's passion for Histotechnology and the outrage that we are not allowed to fully participate in the test system model, but I think we should be directing more of our outrage to the individuals working in Histotechnology that are not and will not take responsibility to increase the professionalism of our profession and our own acceptance of the current state of Histotechnology. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results. As good as we are and as complex parts of the Histotechnology process may be, Histotechnicians, Histotechnologists and Pathology Assistants do not meet the standard stated and do not participate in the post-analytic phase, produce and release patient results. We simply are not able to be credentialed as is the Medical Technologists and Cytotechnologist. I am not saying any one laboratory professional group is better than the other, just that to be considered testing personnel, we must be properly credentialed. Collectively, we as a discipline, science and group should be working to upgrade our education requirements and training so that we can become fully invested partners with the Pathologist. We, not CAP or CLIA, must greatly increase our professionalism before we can truly be considered competent to work in the post-analytical phase. I cannot today accept that every working Histotechnician, Histotechnologist and Pathologist Assistant is able to produce the "result and release". I am quite sure that every Medical Technologist and Cytotechnologist is capable and competent to produce and release a patient result. As things stand today, Histotechnology and all of us the working in this discipline are a support function to the one person in our discipline, the Pathologist, that is educated, trained, credentialed and competent to produce and release a patient result. I also believe there are many opportunities within our process available now, such as histochemical staining for organisms, that could allow us to participate in the post-analytic step. There will be many more as personalized medicine continues to transform Histotechnology. That said, how can we honestly promote our participation in the post-analytic phase, when there are far too many individuals (good, decent and hard working) that work every day, in every type and complexity of lab, that do not have a formal secondary education, have participated in defined clinical trials or have completed a certification exam (required and necessary credentials). Just think how many practitioners of Histotechnology are out there working today that are not properly credentialed. Now think if you know of any Medical Technologist or Cytotechnologist are working that do not have the required credentials. We have many obstacles to increasing the professionalism of Histotechnology; wide and varied backgrounds, lack of standards, lack of automation, lack of certification, but I do not think that CAP or CLIA should be considered one of them. This problem is completely our responsibility. We first have to demand proper credentials, no exceptions, no matter the problem, before we can expect other laboratory professionals to support us in increasing our professionalism and participation in the healthcare delivery system. As important the need for a robust accreditation process, healthy discussion must take place before real change can happen. I suggest we direct our passion and outrage to demand proper credentials to work in Histotechnology and then demand full participation in the test system and proper recognition by all laboratory professionals. William DeSalvo, B.S., HTL(ASCP) > From: JEllin@yumaregional.org > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > Date: Thu, 17 May 2012 17:52:44 +0000 > CC: > Subject: [Histonet] RE: CAP vs. CLIA > > I completely agree with you on this. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, May 17, 2012 10:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > Jesus wrote: > > " I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. " > > Certainly the regulations limit the "high complexity" designation to interpretation of procedure results, but that does not mean a facility does not need very highly trained and competent technologists to do the protocols that lead to good interpretation. It simply highlights the difference between running slides through protocols vs looking at the result and determining a diagnosis. I'm sure most here will see the difference. > > Remember that CAP is a simply a deemed agency of CLIA - that is, CMS (Centers for Medicare and Medicaid, which administers the CLIA regulations) delegates to CAP (and Joint Commission) the authority to accredit laboratories. CAP cannot make up new regulations, only enforce existing CLIA regulations. However, the CLIA regulations are by necessity very general so they can apply to any kind of laboratory operations, current or future. CAP has the leeway to look at what labs are doing and determine if the CLIA regulations apply to those tasks. However, CAP must submit their proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are implemented. > > CAP checklists are far more complex than they were 20 years ago. But the histo lab is far more complex as well, and regulators (as well as the public) are looking much more closely at histology because of some major mistakes that have happened largely due to lack of rigor in testing validation and implementation. A lot of that has to do with small labs doing complex testing (interpretation) with methods they were/are not fully competent to do primarily due to lack of experience and expertise. > > While the accreditation process is getting more onerous, it is also forcing labs to be much more professional in their operations - always a good thing, I think. > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. > Sent: Thursday, May 17, 2012 7:26 AM > To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > Very well said Jesus. I agree. > > Donna Willis, HT/HTL (ASCP) > Histology Lab Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > > -----Original Message----- > From: Jesus Ellin [mailto:JEllin@yumaregional.org] > Sent: Thursday, May 17, 2012 9:24 AM > To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: CAP vs. CLIA > > I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. > > Jesus Ellin HT/PA ASCP, BSBE,MSBE > Yuma Regional Medical Center > Anatomic Pathology Supervisor > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 7:05 AM > To: 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > It was a CAP e alert dated April 2, 2012 > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > > -----Original Message----- > From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] > Sent: Thursday, May 17, 2012 8:42 AM > To: Horn, Hazel V; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: CAP vs. CLIA > > I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. > > This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Histology Lab Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 7:26 AM > To: 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: > > " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? > > As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." > > And goes on to say: > "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? > > Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce > Sent: Wednesday, May 16, 2012 2:27 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP vs. CLIA > > > Can someone help me with the High Complexity Test with CAP vs. CLIA. > Thanks > Courtney Pierce > IHC Specialist > Quintiles > Translational R&D - Oncology > Innovation > Navigating the new health > > 610 Oakmont Lane > Westmont, IL 60559 > > Office: + 630-203-6234 > courtney.pierce@quintiles.com > > clinical | commercial | consulting | capital > > > ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. > ************************************************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.boanas <@t> epistem.co.uk Mon May 21 01:59:47 2012 From: a.boanas <@t> epistem.co.uk (Adam Boanas) Date: Mon May 21 01:59:56 2012 Subject: [Histonet] Translucent patch Message-ID: <176C97AAA877D24798ED7376652D0FD8178CE0F0B2@SRVEXCH02.epistem.local> Hello, We have recently noticed strange translucent patches that can be seen within our 3 micron tissue sections when they are floated out. From the surface of the water bath they look like holes within the tissue but when the slides are viewed, the tissue is still present. This region of tissue however, creases and folds when mounted. The rest of the section looks and behaves perfectly - it is only this translucent region that is causing the problem. I have seen this region as a small blob but also as a thin streak that runs through the entire section. This region can only be seen for the first 7-10 sections taken. After this the translucent region gets smaller and disappears. This is making getting perfectly flat, artefact free sections of tissue (mouse / rat gut / liver) difficult. Does anyone have any idea what this could be? My thoughts are a possibly processing issue - currently process without vacuum wax infiltration or an embedding issue. Our metal moulds were cleaned with a methanol based para-release spray about 2 months ago by mistake - we have had subsequent tissue spreading issues as a result. Could traces of this affect the cutting surface of the tissue causing this patch? Any ideas would be great, Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX From sllandsc <@t> gmail.com Mon May 21 07:32:05 2012 From: sllandsc <@t> gmail.com (Sara Landschoot) Date: Mon May 21 07:32:11 2012 Subject: [Histonet] Manual for Sorvall MT-2B Rotary Motor UltraMicrotome? Message-ID: Does anyone have a manual for the Sorvall MT-2B rotary motor ultramicrotome? I have been asked to teach a student how to use it for their research, yet I have never used this ultramicrotome before. Thank you, Sara Landschoot HTL(ASCP) Medical College of Wisconsin Orthopaedic Surgery - Research From brendal.finlay <@t> medicalcenterclinic.com Mon May 21 09:25:21 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Mon May 21 09:25:35 2012 Subject: [Histonet] Help Message-ID: Nancy, We've had similar issues with fatty tissue falling off of the slides while performing IHC.? We use Superfrost + slides which we have found to really hold the tissue well.? Also, I have learned through reading round on the?Histonet?that air drying doesn't completely remove the water from the middle area of a tissue section.? For this reason, we no longer air dry at all unless it's a slide that was cut the day before and just happened to be air dried.? Our protocol changed to cutting the slides and draining them well, then putting them in a 60 C oven for 15 minutes.??Then?the slides are run?down to water on an automated stainer with another 15 minute time in the oven on the stainer.? A specific instance?when the tissue falls off,?was during antigen retrieval in Trilogy in a pressure cooker.? If the pressure was manually released, this would cause the Trilogy to boil?and?it would separate the tissue from the slide.? Ourprotocol changed to 12 minutes in the pressure cooker with Trilogy, then around 8 minutes?to wait for the pressure to release on it's own.? We would then rinse softly in distilled water to remove the?Trilogy.? This also seemed to help with the issue. ? The combination of this?has worked?fairly well for us with some exceptionally stubborn tissue still?attempting to fall off of the slides.??I would love to?hear of other's experiences and?how they resolved this.?? I do wonder about the length of time in your oven.? I had spoken with one of our Biocare reps about this when we encountered the problem and he felt that longer than 30 minutes in the oven would damage the specimen's IHC integrity.? Brendal Finlay HT (ASCP) ----Original message----- From: "Cloughley-Gray, Nancy" CloughleyN@rvh.on.ca Date: Fri, 18 May 2012 14:02:35 -0500 To: "'histonet@lists.utsouthwestern.edu'"histonet@lists.utsouthwestern.edu Subject: [Histonet] Help > I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? > I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). > I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. > > Thanking you in advance, > I look forward to your input, > Nancy Cloughley-Gray MLT > From mwhite <@t> mcleodhealth.org Mon May 21 09:50:42 2012 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Mon May 21 09:50:51 2012 Subject: [Histonet] histotech position Message-ID: I know of a histotech position available in South Carolina. Are there any histonetters looking for work in this area? Melanie NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. From akelley1 <@t> slu.edu Mon May 21 09:53:41 2012 From: akelley1 <@t> slu.edu (Amanda Kelley) Date: Mon May 21 09:53:54 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt> <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: I usually do not comment on the histonet, however this topic is near and dear to my heart. We in Histology are support scientific staff to the Pathologist as much as the Laboratory director is to the Pathologist. Scientific duties, which a pathologist can perform (histological and cytological preparation has been delegated down) to our position. Interpretation of Controls and associated material is often left to us and the Pathologist assistant We determine what the Pathologist can interpret Our expertise directly determines patient outcome, if we are not professional in our decisions then the Pathologist is ineffective in his or hers. The Professional business of running the laboratory is performed by the medical laboratory director, and associate director. These are professional positions within the laboratory where a Pathologist has delegated the managerial responsibility to a subordinate. According to CLIA, this position does not exist, only the Pathologist can truly be the Medical laboratory director. Yet in hospitals across the country many Clinical laboratory Scientist's who don't know anything or have limited knowledge of AP are in charge of our AP labs.Their positions are listed as Laboratory Director. This subordinate is the most important person in the lab, yet they too do not turn out results. They are frequently the highest paid in the lab. Their pay is based on the designation of their scientific and managerial background. I believe we can do the same. The business of the pathology lab would be very difficult for the pathologist to handle without this symbiotic relationship of the scientific businessman to the Pathologist. Likewise a Pathologist can not perform their job without the symbiotic relationship to the histotechnologist.Thereby technically, making our jobs indispensable to the Pathologist much like the laboratory director. Our problem is history, where many pathologists trained their out of work brother in law to do the work. Many rural areas still run their labs this way. As long as there is a path to become a histotech by hiring whomsoever to do the job. We will always be left behind. So I believe if CLIA and CAP can recognize the laboratory Manager as an important professional position delegated by the Pathologist then we should be afforded the same courtesy. On Sun, May 20, 2012 at 10:37 PM, WILLIAM DESALVO wrote: > > > > > > > > > > > I seemed to have missed something or it might have been all the fresh sea > air I got in Tampa at the FSH, but I do not understand the outrage > expressed towards CLIA and CAP because we are not listed as testing > personnel. I applaud everyone's passion for Histotechnology and the outrage > that we are not allowed to fully participate in the test system model, but > I think we should be directing more of our outrage to the individuals > working in Histotechnology that are not and will not take responsibility to > increase the professionalism of our profession and our own acceptance of > the current state of Histotechnology. > > A TEST SYSTEM is the process that includes pre-analytic, analytic, and > post-analytic steps used to produce a test result or set of results. As > good as we are and as complex parts of the Histotechnology process may be, > Histotechnicians, Histotechnologists and Pathology Assistants do not meet > the standard stated and do not participate in the post-analytic phase, > produce and release patient results. We simply are not able to be > credentialed as is the Medical Technologists and Cytotechnologist. I am not > saying any one laboratory professional group is better than the other, just > that to be considered testing personnel, we must be properly credentialed. > Collectively, we as a discipline, science and group should be working to > upgrade our education requirements and training so that we can become fully > invested partners with the Pathologist. We, not CAP or CLIA, must greatly > increase our professionalism before we can truly be considered competent to > work in the post-analytical phase. I cannot today accept that every working > Histotechnician, Histotechnologist and Pathologist Assistant is able to > produce the "result and release". I am quite sure that every Medical > Technologist and Cytotechnologist is capable and competent to produce and > release a patient result. As things stand today, Histotechnology and all of > us the working in this discipline are a support function to the one person > in our discipline, the Pathologist, that is educated, trained, credentialed > and competent to produce and release a patient result. I also believe there > are many opportunities within our process available now, such as > histochemical staining for organisms, that could allow us to participate in > the post-analytic step. There will be many more as personalized medicine > continues to transform Histotechnology. That said, how can we honestly > promote our participation in the post-analytic phase, when there are far > too many individuals (good, decent and hard working) that work every day, > in every type and complexity of lab, that do not have a formal secondary > education, have participated in defined clinical trials or have completed a > certification exam (required and necessary credentials). Just think how > many practitioners of Histotechnology are out there working today that are > not properly credentialed. Now think if you know of any Medical > Technologist or Cytotechnologist are working that do not have the required > credentials. We have many obstacles to increasing the professionalism of > Histotechnology; wide and varied backgrounds, lack of standards, lack of > automation, lack of certification, but I do not think that CAP or CLIA > should be considered one of them. This problem is completely our > responsibility. We first have to demand proper credentials, no exceptions, > no matter the problem, before we can expect other laboratory professionals > to support us in increasing our professionalism and participation in the > healthcare delivery system. As important the need for a robust > accreditation process, healthy discussion must take place before real > change can happen. I suggest we direct our passion and outrage to demand > proper credentials to work in Histotechnology and then demand full > participation in the test system and proper recognition by all laboratory > professionals. > > > William DeSalvo, B.S., HTL(ASCP) > > > > From: JEllin@yumaregional.org > > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > > Date: Thu, 17 May 2012 17:52:44 +0000 > > CC: > > Subject: [Histonet] RE: CAP vs. CLIA > > > > I completely agree with you on this. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > > Sent: Thursday, May 17, 2012 10:46 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > Jesus wrote: > > > > " I think the CAP need to re-evaluate this and re consider what high > complexity testing is, because CLIA defines it not the CAP. Remember CAP > enforces CLIA regulation as well as their own. " > > > > Certainly the regulations limit the "high complexity" designation to > interpretation of procedure results, but that does not mean a facility does > not need very highly trained and competent technologists to do the > protocols that lead to good interpretation. It simply highlights the > difference between running slides through protocols vs looking at the > result and determining a diagnosis. I'm sure most here will see the > difference. > > > > Remember that CAP is a simply a deemed agency of CLIA - that is, CMS > (Centers for Medicare and Medicaid, which administers the CLIA regulations) > delegates to CAP (and Joint Commission) the authority to accredit > laboratories. CAP cannot make up new regulations, only enforce existing > CLIA regulations. However, the CLIA regulations are by necessity very > general so they can apply to any kind of laboratory operations, current or > future. CAP has the leeway to look at what labs are doing and determine if > the CLIA regulations apply to those tasks. However, CAP must submit their > proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are > implemented. > > > > CAP checklists are far more complex than they were 20 years ago. But the > histo lab is far more complex as well, and regulators (as well as the > public) are looking much more closely at histology because of some major > mistakes that have happened largely due to lack of rigor in testing > validation and implementation. A lot of that has to do with small labs > doing complex testing (interpretation) with methods they were/are not fully > competent to do primarily due to lack of experience and expertise. > > > > While the accreditation process is getting more onerous, it is also > forcing labs to be much more professional in their operations - always a > good thing, I think. > > > > Tim Morken > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. > > Sent: Thursday, May 17, 2012 7:26 AM > > To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > Very well said Jesus. I agree. > > > > Donna Willis, HT/HTL (ASCP) > > Histology Lab Manager > > Baylor University Medical Center-Dallas > > ph. 214-820-2465 office > > ph. 214-725-6184 mobile > > donna.willis@baylorhealth.edu > > > > > > -----Original Message----- > > From: Jesus Ellin [mailto:JEllin@yumaregional.org] > > Sent: Thursday, May 17, 2012 9:24 AM > > To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: CAP vs. CLIA > > > > I am going to have to go there,, sorry all I know I am going to stir-up > a hornets nest, but here it goes, don't we think that this is done in lue > of the fact that CAP are representing the Pathologist interest and not the > interest of the Technicians. Times have changed and the CAP is asking for > more and more from Anatomic Pathology questions every year, not only to > include technical, but also instrumentation (simple and complex), as well > as information systems, predictive markers, Digital Pathology ( a huge > one), etc. I think the CAP need to re-evaluate this and re consider what > high complexity testing is, because CLIA defines it not the CAP. Remember > CAP enforces CLIA regulation as well as their own. I would challenge this. > I feel the staff under me do more than turn a wheel, or place tissue in a > mold. With Passion comes a need to start to create change, we need this > done. > > > > Jesus Ellin HT/PA ASCP, BSBE,MSBE > > Yuma Regional Medical Center > > Anatomic Pathology Supervisor > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > > Sent: Thursday, May 17, 2012 7:05 AM > > To: 'Willis, Donna G.'; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > It was a CAP e alert dated April 2, 2012 > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > > > -----Original Message----- > > From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] > > Sent: Thursday, May 17, 2012 8:42 AM > > To: Horn, Hazel V; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: CAP vs. CLIA > > > > I have to say I disagree with this interpretation. The commentary in > the 7/11/2011 checklists indicates that regulations apply to "A laboratory > must evaluate and document the competency of all testing personnel for each > test system. A TEST SYSTEM is the process that includes pre-analytic, > analytic, and post-analytic steps used to produce a test result or set of > results." To me this includes both histology and pathology office staff. > > > > This is the opinion on myself and our compliance person. Hazel can you > tell us where to find the CAP quote. > > > > Thanks, > > > > Donna Willis, HT/HTL (ASCP) > > Histology Lab Manager > > Baylor University Medical Center-Dallas > > ph. 214-820-2465 office > > ph. 214-725-6184 mobile > > donna.willis@baylorhealth.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > > Sent: Thursday, May 17, 2012 7:26 AM > > To: 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > CAP does not consider us testing personnel. How they come by this is a > mystery to me. In a recent memo from CAP it describes this: > > > > " Why does CAP require the completion of the "Laboratory Personnel > Evaluation Roster" form and when was this process implemented? > > > > As part of CAP's deemed status with CMS as an accrediting organization, > CMS required CAP to implement a more stringent process to document that > accredited laboratories have appropriately qualified personnel and adequate > documentation of personnel qualifications. The Laboratory Personnel > Evaluation Roster form requires laboratories to confirm that personnel > files contain the information necessary for laboratories to be in > compliance with the CLIA personnel qualification regulations and CAP > Checklist requirements prior to the inspection. It is also used by the > inspection team to assist in the auditing of the records during the > inspection to confirm compliance with the Checklist requirements. The > process of completing the personnel form took effect in August 2009." > > > > And goes on to say: > > "Do I need to list histologists on the Laboratory Personnel Evaluation > Roster? > > > > Typical histologist duties (e.g., fixation, embedding, microtomy, > staining and cover slipping) are not considered testing. Therefore, it is > not necessary to list these personnel on the roster. However, if the > histologist is performing any part of the macroscopic tissue examination > which is considered high complexity testing, it is necessary to list those > personnel on the roster. Such personnel must provide documentation at > minimum of an associate's degree/transcripts or high school diploma or > equivalent for individuals performing grossing at the same laboratory prior > to September 1, 1997." > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce > > Sent: Wednesday, May 16, 2012 2:27 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] CAP vs. CLIA > > > > > > Can someone help me with the High Complexity Test with CAP vs. CLIA. > > Thanks > > Courtney Pierce > > IHC Specialist > > Quintiles > > Translational R&D - Oncology > > Innovation > > Navigating the new health > > > > 610 Oakmont Lane > > Westmont, IL 60559 > > > > Office: + 630-203-6234 > > courtney.pierce@quintiles.com > > > > clinical | commercial | consulting | capital > > > > > > ********************** IMPORTANT--PLEASE READ ************************ > This electronic message, including its attachments, is COMPANY CONFIDENTIAL > and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are > not the intended recipient, you are hereby notified that any use, > disclosure, copying, or distribution of this message or any of the > information included in it is unauthorized and strictly prohibited. If you > have received this message in error, please immediately notify the sender > by reply e-mail and permanently delete this message and its attachments, > along with any copies thereof. Thank you. > > ************************************************************************ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message > is not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it > from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************** > > This e-mail may contain confidential and/or privileged information. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly forbidden and possibly a violation of federal or state law and > regulations. Baylor Health Care System, its subsidiaries, and affiliates > hereby claim all applicable privileges related to this information. > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message > is not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it > from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, or > copying of this message is strictly prohibited. If you receive this > message in error, or are not the named recipient(s), please notify the > sender at either the e-mail, fax, address, or telephone number listed above > and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. 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From JEllin <@t> yumaregional.org Mon May 21 10:04:02 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon May 21 10:04:15 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: , <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org>, <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt>, <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org>, , <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt>, <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org>, Message-ID: Bill I have to agree with you on this, but then again we have always been looked as a step children within the lab. What I see is word play here, Cyto tech and Med Tech (CLS) are to be credited with release of a result. But because there Tech ID number is on the result they are accountable for this. As we move forward in the computer age within Anatomic Pathology we are going to be seeing the same shift, but we need our professional societies, to start to transform our profession. I am talking about algorithm analysis, special stains, IHC, bio banking, etc. There are many decisions that make us more than just a point and push tech, for lack there of a better term. I do agree education is a barrier, but once again how did the CLS (Med Tech), Cyto tech evelove? I do recall when they were taught on the job or through military training, so to say they are better because of a degree is far from the truth. Many MANY tech these days are assets to our profession and as we move forward in the future they we need to look for ways to have properly credentialed and EXPERIANCED staff. I my self am witness to the lack of basic lab knowledge a new grads have, but we are also responsible because our clinical rotation programs are scares and we do not have time to train. The future is full of opportunity for all histology tech, educated and experience, we just need to move forward and have the healthy discussion and make the changes needed in order to establish our profession. ________________________________ From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Sunday, May 20, 2012 8:38 PM To: Jesus Ellin; Timothy Morken; histonet Subject: RE: [Histonet] RE: CAP vs. CLIA I seemed to have missed something or it might have been all the fresh sea air I got in Tampa at the FSH, but I do not understand the outrage expressed towards CLIA and CAP because we are not listed as testing personnel. I applaud everyone's passion for Histotechnology and the outrage that we are not allowed to fully participate in the test system model, but I think we should be directing more of our outrage to the individuals working in Histotechnology that are not and will not take responsibility to increase the professionalism of our profession and our own acceptance of the current state of Histotechnology. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results. As good as we are and as complex parts of the Histotechnology process may be, Histotechnicians, Histotechnologists and Pathology Assistants do not meet the standard stated and do not participate in the post-analytic phase, produce and release patient results. We simply are not able to be credentialed as is the Medical Technologists and Cytotechnologist. I am not saying any one laboratory professional group is better than the other, just that to be considered testing personnel, we must be properly credentialed. Collectively, we as a discipline, science and group should be working to upgrade our education requirements and training so that we can become fully invested partners with the Pathologist. We, not CAP or CLIA, must greatly increase our professionalism before we can truly be considered competent to work in the post-analytical phase. I cannot today accept that every working Histotechnician, Histotechnologist and Pathologist Assistant is able to produce the "result and release". I am quite sure that every Medical Technologist and Cytotechnologist is capable and competent to produce and release a patient result. As things stand today, Histotechnology and all of us the working in this discipline are a support function to the one person in our discipline, the Pathologist, that is educated, trained, credentialed and competent to produce and release a patient result. I also believe there are many opportunities within our process available now, such as histochemical staining for organisms, that could allow us to participate in the post-analytic step. There will be many more as personalized medicine continues to transform Histotechnology. That said, how can we honestly promote our participation in the post-analytic phase, when there are far too many individuals (good, decent and hard working) that work every day, in every type and complexity of lab, that do not have a formal secondary education, have participated in defined clinical trials or have completed a certification exam (required and necessary credentials). Just think how many practitioners of Histotechnology are out there working today that are not properly credentialed. Now think if you know of any Medical Technologist or Cytotechnologist are working that do not have the required credentials. We have many obstacles to increasing the professionalism of Histotechnology; wide and varied backgrounds, lack of standards, lack of automation, lack of certification, but I do not think that CAP or CLIA should be considered one of them. This problem is completely our responsibility. We first have to demand proper credentials, no exceptions, no matter the problem, before we can expect other laboratory professionals to support us in increasing our professionalism and participation in the healthcare delivery system. As important the need for a robust accreditation process, healthy discussion must take place before real change can happen. I suggest we direct our passion and outrage to demand proper credentials to work in Histotechnology and then demand full participation in the test system and proper recognition by all laboratory professionals. William DeSalvo, B.S., HTL(ASCP) > From: JEllin@yumaregional.org > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > Date: Thu, 17 May 2012 17:52:44 +0000 > CC: > Subject: [Histonet] RE: CAP vs. CLIA > > I completely agree with you on this. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, May 17, 2012 10:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > Jesus wrote: > > " I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. " > > Certainly the regulations limit the "high complexity" designation to interpretation of procedure results, but that does not mean a facility does not need very highly trained and competent technologists to do the protocols that lead to good interpretation. It simply highlights the difference between running slides through protocols vs looking at the result and determining a diagnosis. I'm sure most here will see the difference. > > Remember that CAP is a simply a deemed agency of CLIA - that is, CMS (Centers for Medicare and Medicaid, which administers the CLIA regulations) delegates to CAP (and Joint Commission) the authority to accredit laboratories. CAP cannot make up new regulations, only enforce existing CLIA regulations. However, the CLIA regulations are by necessity very general so they can apply to any kind of laboratory operations, current or future. CAP has the leeway to look at what labs are doing and determine if the CLIA regulations apply to those tasks. However, CAP must submit their proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are implemented. > > CAP checklists are far more complex than they were 20 years ago. But the histo lab is far more complex as well, and regulators (as well as the public) are looking much more closely at histology because of some major mistakes that have happened largely due to lack of rigor in testing validation and implementation. A lot of that has to do with small labs doing complex testing (interpretation) with methods they were/are not fully competent to do primarily due to lack of experience and expertise. > > While the accreditation process is getting more onerous, it is also forcing labs to be much more professional in their operations - always a good thing, I think. > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. > Sent: Thursday, May 17, 2012 7:26 AM > To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > Very well said Jesus. I agree. > > Donna Willis, HT/HTL (ASCP) > Histology Lab Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > > -----Original Message----- > From: Jesus Ellin [mailto:JEllin@yumaregional.org] > Sent: Thursday, May 17, 2012 9:24 AM > To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: CAP vs. CLIA > > I am going to have to go there,, sorry all I know I am going to stir-up a hornets nest, but here it goes, don't we think that this is done in lue of the fact that CAP are representing the Pathologist interest and not the interest of the Technicians. Times have changed and the CAP is asking for more and more from Anatomic Pathology questions every year, not only to include technical, but also instrumentation (simple and complex), as well as information systems, predictive markers, Digital Pathology ( a huge one), etc. I think the CAP need to re-evaluate this and re consider what high complexity testing is, because CLIA defines it not the CAP. Remember CAP enforces CLIA regulation as well as their own. I would challenge this. I feel the staff under me do more than turn a wheel, or place tissue in a mold. With Passion comes a need to start to create change, we need this done. > > Jesus Ellin HT/PA ASCP, BSBE,MSBE > Yuma Regional Medical Center > Anatomic Pathology Supervisor > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 7:05 AM > To: 'Willis, Donna G.'; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > It was a CAP e alert dated April 2, 2012 > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > > -----Original Message----- > From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] > Sent: Thursday, May 17, 2012 8:42 AM > To: Horn, Hazel V; 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: CAP vs. CLIA > > I have to say I disagree with this interpretation. The commentary in the 7/11/2011 checklists indicates that regulations apply to "A laboratory must evaluate and document the competency of all testing personnel for each test system. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results." To me this includes both histology and pathology office staff. > > This is the opinion on myself and our compliance person. Hazel can you tell us where to find the CAP quote. > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Histology Lab Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Thursday, May 17, 2012 7:26 AM > To: 'Courtney Pierce' > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP vs. CLIA > > CAP does not consider us testing personnel. How they come by this is a mystery to me. In a recent memo from CAP it describes this: > > " Why does CAP require the completion of the "Laboratory Personnel Evaluation Roster" form and when was this process implemented? > > As part of CAP's deemed status with CMS as an accrediting organization, CMS required CAP to implement a more stringent process to document that accredited laboratories have appropriately qualified personnel and adequate documentation of personnel qualifications. The Laboratory Personnel Evaluation Roster form requires laboratories to confirm that personnel files contain the information necessary for laboratories to be in compliance with the CLIA personnel qualification regulations and CAP Checklist requirements prior to the inspection. It is also used by the inspection team to assist in the auditing of the records during the inspection to confirm compliance with the Checklist requirements. The process of completing the personnel form took effect in August 2009." > > And goes on to say: > "Do I need to list histologists on the Laboratory Personnel Evaluation Roster? > > Typical histologist duties (e.g., fixation, embedding, microtomy, staining and cover slipping) are not considered testing. Therefore, it is not necessary to list these personnel on the roster. However, if the histologist is performing any part of the macroscopic tissue examination which is considered high complexity testing, it is necessary to list those personnel on the roster. Such personnel must provide documentation at minimum of an associate's degree/transcripts or high school diploma or equivalent for individuals performing grossing at the same laboratory prior to September 1, 1997." > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce > Sent: Wednesday, May 16, 2012 2:27 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP vs. CLIA > > > Can someone help me with the High Complexity Test with CAP vs. CLIA. > Thanks > Courtney Pierce > IHC Specialist > Quintiles > Translational R&D - Oncology > Innovation > Navigating the new health > > 610 Oakmont Lane > Westmont, IL 60559 > > Office: + 630-203-6234 > courtney.pierce@quintiles.com > > clinical | commercial | consulting | capital > > > ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. 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Thank You. ______________________________________________________________________ From pathlocums <@t> gmail.com Mon May 21 10:35:35 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon May 21 10:35:46 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: References: <25A4DE08332B19499904459F00AAACB719BB4A1603@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C8B@BHDAEXVM33.bhcs.pvt> <25A4DE08332B19499904459F00AAACB719BB4A1604@EVS1.archildrens.org> <3FA597486B013249A2FC8EE113CBCC02196A617C92@BHDAEXVM33.bhcs.pvt> <8D7C2D242DBD45498006B21122072BF8B4F4E5F7@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: William, I certainly agree that folks in both histology and pathology disciplines need to work harder at defining who they are as professionals. We, in both departments, are responsible for creating confusion. It is no wonder progress is slow in this area. If we all want to be treated more as professionals, and become better recognized, then we should start correcting the problems we allow to exist. The best place to start is with titles. - A person should not be called a "histotech" unless they are certified. Anyone practicing histology that is not certified should be called something else - perhaps lab assistant, or histology assistant. Regardless of experience, the tem HT should be reserved for those that are certified. If not, what is the value of certification? - Nobody should use the phrase "PA" or "Pathologists' Assistant" if they are not certified. I cannot tell you how many times I hear about the "PA" at this place, or that place and when I check the registry those folks are not PA's at all. This gets under the skin of each and every one of us that went through years of training, and received graduate degrees to claim that title, and is unfair to all the others that have thier degrees and completed all the requirements set forth by the ASCP and the AAPA. It seems that many like to self-promote themselves to titles they never earned. We cannot take a title simply because it is the closest description to what we do all day. I have a graduate degree in pathology, but I would never think of calling myself a "pathologist". And I would be more accurate with that title than most that call themselves PA's. A graduate degree in microbiology? A Microbiologist. Graduate degree in psychology? A Psychologist. Graduate degree in biology? A Biologist. Graduate degree in Pathology????? A PA. Why? Because the title Pathologist is reserved for a very specific person, with a very specific training and certification. So too should be the title HT and PA. A widespread problem in medicine is that of folks pretending to be what they are not. Some clarification is in order in all areas of medicine. Why don't we correct the problems in our own little corner and set an example? Everybody can be what they want to be, but today - you are what you are (generalization, not YOU) On Sun, May 20, 2012 at 8:37 PM, WILLIAM DESALVO wrote: > > > > > > > > > > > I seemed to have missed something or it might have been all the fresh sea > air I got in Tampa at the FSH, but I do not understand the outrage > expressed towards CLIA and CAP because we are not listed as testing > personnel. I applaud everyone's passion for Histotechnology and the outrage > that we are not allowed to fully participate in the test system model, but > I think we should be directing more of our outrage to the individuals > working in Histotechnology that are not and will not take responsibility to > increase the professionalism of our profession and our own acceptance of > the current state of Histotechnology. > > A TEST SYSTEM is the process that includes pre-analytic, analytic, and > post-analytic steps used to produce a test result or set of results. As > good as we are and as complex parts of the Histotechnology process may be, > Histotechnicians, Histotechnologists and Pathology Assistants do not meet > the standard stated and do not participate in the post-analytic phase, > produce and release patient results. We simply are not able to be > credentialed as is the Medical Technologists and Cytotechnologist. I am not > saying any one laboratory professional group is better than the other, just > that to be considered testing personnel, we must be properly credentialed. > Collectively, we as a discipline, science and group should be working to > upgrade our education requirements and training so that we can become fully > invested partners with the Pathologist. We, not CAP or CLIA, must greatly > increase our professionalism before we can truly be considered competent to > work in the post-analytical phase. I cannot today accept that every working > Histotechnician, Histotechnologist and Pathologist Assistant is able to > produce the "result and release". I am quite sure that every Medical > Technologist and Cytotechnologist is capable and competent to produce and > release a patient result. As things stand today, Histotechnology and all of > us the working in this discipline are a support function to the one person > in our discipline, the Pathologist, that is educated, trained, credentialed > and competent to produce and release a patient result. I also believe there > are many opportunities within our process available now, such as > histochemical staining for organisms, that could allow us to participate in > the post-analytic step. There will be many more as personalized medicine > continues to transform Histotechnology. That said, how can we honestly > promote our participation in the post-analytic phase, when there are far > too many individuals (good, decent and hard working) that work every day, > in every type and complexity of lab, that do not have a formal secondary > education, have participated in defined clinical trials or have completed a > certification exam (required and necessary credentials). Just think how > many practitioners of Histotechnology are out there working today that are > not properly credentialed. Now think if you know of any Medical > Technologist or Cytotechnologist are working that do not have the required > credentials. We have many obstacles to increasing the professionalism of > Histotechnology; wide and varied backgrounds, lack of standards, lack of > automation, lack of certification, but I do not think that CAP or CLIA > should be considered one of them. This problem is completely our > responsibility. We first have to demand proper credentials, no exceptions, > no matter the problem, before we can expect other laboratory professionals > to support us in increasing our professionalism and participation in the > healthcare delivery system. As important the need for a robust > accreditation process, healthy discussion must take place before real > change can happen. I suggest we direct our passion and outrage to demand > proper credentials to work in Histotechnology and then demand full > participation in the test system and proper recognition by all laboratory > professionals. > > > William DeSalvo, B.S., HTL(ASCP) > > > > From: JEllin@yumaregional.org > > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > > Date: Thu, 17 May 2012 17:52:44 +0000 > > CC: > > Subject: [Histonet] RE: CAP vs. CLIA > > > > I completely agree with you on this. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > > Sent: Thursday, May 17, 2012 10:46 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > Jesus wrote: > > > > " I think the CAP need to re-evaluate this and re consider what high > complexity testing is, because CLIA defines it not the CAP. Remember CAP > enforces CLIA regulation as well as their own. " > > > > Certainly the regulations limit the "high complexity" designation to > interpretation of procedure results, but that does not mean a facility does > not need very highly trained and competent technologists to do the > protocols that lead to good interpretation. It simply highlights the > difference between running slides through protocols vs looking at the > result and determining a diagnosis. I'm sure most here will see the > difference. > > > > Remember that CAP is a simply a deemed agency of CLIA - that is, CMS > (Centers for Medicare and Medicaid, which administers the CLIA regulations) > delegates to CAP (and Joint Commission) the authority to accredit > laboratories. CAP cannot make up new regulations, only enforce existing > CLIA regulations. However, the CLIA regulations are by necessity very > general so they can apply to any kind of laboratory operations, current or > future. CAP has the leeway to look at what labs are doing and determine if > the CLIA regulations apply to those tasks. However, CAP must submit their > proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are > implemented. > > > > CAP checklists are far more complex than they were 20 years ago. But the > histo lab is far more complex as well, and regulators (as well as the > public) are looking much more closely at histology because of some major > mistakes that have happened largely due to lack of rigor in testing > validation and implementation. A lot of that has to do with small labs > doing complex testing (interpretation) with methods they were/are not fully > competent to do primarily due to lack of experience and expertise. > > > > While the accreditation process is getting more onerous, it is also > forcing labs to be much more professional in their operations - always a > good thing, I think. > > > > Tim Morken > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. > > Sent: Thursday, May 17, 2012 7:26 AM > > To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > Very well said Jesus. I agree. > > > > Donna Willis, HT/HTL (ASCP) > > Histology Lab Manager > > Baylor University Medical Center-Dallas > > ph. 214-820-2465 office > > ph. 214-725-6184 mobile > > donna.willis@baylorhealth.edu > > > > > > -----Original Message----- > > From: Jesus Ellin [mailto:JEllin@yumaregional.org] > > Sent: Thursday, May 17, 2012 9:24 AM > > To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: CAP vs. CLIA > > > > I am going to have to go there,, sorry all I know I am going to stir-up > a hornets nest, but here it goes, don't we think that this is done in lue > of the fact that CAP are representing the Pathologist interest and not the > interest of the Technicians. Times have changed and the CAP is asking for > more and more from Anatomic Pathology questions every year, not only to > include technical, but also instrumentation (simple and complex), as well > as information systems, predictive markers, Digital Pathology ( a huge > one), etc. I think the CAP need to re-evaluate this and re consider what > high complexity testing is, because CLIA defines it not the CAP. Remember > CAP enforces CLIA regulation as well as their own. I would challenge this. > I feel the staff under me do more than turn a wheel, or place tissue in a > mold. With Passion comes a need to start to create change, we need this > done. > > > > Jesus Ellin HT/PA ASCP, BSBE,MSBE > > Yuma Regional Medical Center > > Anatomic Pathology Supervisor > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > > Sent: Thursday, May 17, 2012 7:05 AM > > To: 'Willis, Donna G.'; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > It was a CAP e alert dated April 2, 2012 > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > > > -----Original Message----- > > From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] > > Sent: Thursday, May 17, 2012 8:42 AM > > To: Horn, Hazel V; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: CAP vs. CLIA > > > > I have to say I disagree with this interpretation. The commentary in > the 7/11/2011 checklists indicates that regulations apply to "A laboratory > must evaluate and document the competency of all testing personnel for each > test system. A TEST SYSTEM is the process that includes pre-analytic, > analytic, and post-analytic steps used to produce a test result or set of > results." To me this includes both histology and pathology office staff. > > > > This is the opinion on myself and our compliance person. Hazel can you > tell us where to find the CAP quote. > > > > Thanks, > > > > Donna Willis, HT/HTL (ASCP) > > Histology Lab Manager > > Baylor University Medical Center-Dallas > > ph. 214-820-2465 office > > ph. 214-725-6184 mobile > > donna.willis@baylorhealth.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > > Sent: Thursday, May 17, 2012 7:26 AM > > To: 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > CAP does not consider us testing personnel. How they come by this is a > mystery to me. In a recent memo from CAP it describes this: > > > > " Why does CAP require the completion of the "Laboratory Personnel > Evaluation Roster" form and when was this process implemented? > > > > As part of CAP's deemed status with CMS as an accrediting organization, > CMS required CAP to implement a more stringent process to document that > accredited laboratories have appropriately qualified personnel and adequate > documentation of personnel qualifications. The Laboratory Personnel > Evaluation Roster form requires laboratories to confirm that personnel > files contain the information necessary for laboratories to be in > compliance with the CLIA personnel qualification regulations and CAP > Checklist requirements prior to the inspection. It is also used by the > inspection team to assist in the auditing of the records during the > inspection to confirm compliance with the Checklist requirements. The > process of completing the personnel form took effect in August 2009." > > > > And goes on to say: > > "Do I need to list histologists on the Laboratory Personnel Evaluation > Roster? > > > > Typical histologist duties (e.g., fixation, embedding, microtomy, > staining and cover slipping) are not considered testing. Therefore, it is > not necessary to list these personnel on the roster. However, if the > histologist is performing any part of the macroscopic tissue examination > which is considered high complexity testing, it is necessary to list those > personnel on the roster. Such personnel must provide documentation at > minimum of an associate's degree/transcripts or high school diploma or > equivalent for individuals performing grossing at the same laboratory prior > to September 1, 1997." > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce > > Sent: Wednesday, May 16, 2012 2:27 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] CAP vs. CLIA > > > > > > Can someone help me with the High Complexity Test with CAP vs. CLIA. > > Thanks > > Courtney Pierce > > IHC Specialist > > Quintiles > > Translational R&D - Oncology > > Innovation > > Navigating the new health > > > > 610 Oakmont Lane > > Westmont, IL 60559 > > > > Office: + 630-203-6234 > > courtney.pierce@quintiles.com > > > > clinical | commercial | consulting | capital > > > > > > ********************** IMPORTANT--PLEASE READ ************************ > This electronic message, including its attachments, is COMPANY CONFIDENTIAL > and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are > not the intended recipient, you are hereby notified that any use, > disclosure, copying, or distribution of this message or any of the > information included in it is unauthorized and strictly prohibited. If you > have received this message in error, please immediately notify the sender > by reply e-mail and permanently delete this message and its attachments, > along with any copies thereof. Thank you. > > ************************************************************************ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message > is not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it > from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************** > > This e-mail may contain confidential and/or privileged information. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly forbidden and possibly a violation of federal or state law and > regulations. Baylor Health Care System, its subsidiaries, and affiliates > hereby claim all applicable privileges related to this information. > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message > is not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it > from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, or > copying of this message is strictly prohibited. If you receive this > message in error, or are not the named recipient(s), please notify the > sender at either the e-mail, fax, address, or telephone number listed above > and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From PMonfils <@t> Lifespan.org Mon May 21 11:02:19 2012 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon May 21 11:02:28 2012 Subject: [Histonet] Help In-Reply-To: <4E09C2A3596693458A4BDDD2245F3146138A30@RVH-EXMB2.rvh.on.ca> References: <4E09C2A3596693458A4BDDD2245F3146138A30@RVH-EXMB2.rvh.on.ca> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835D2E@LSRIEXCH1.lsmaster.lifespan.org> I realize that such "+" slides come with the instruction to completely dry slides at room temperature before placing in the drying oven. I have used these slides for many years, and have found this procedure to be not only unnecessary, but sometimes problematic. I believe sections are more likely to detach if dried at room temperature prior to oven drying. If the section is not lying perfectly flat against the glass - and some types of tissue never do initially - room temperature drying doesn't allow wrinkled areas or other problem areas to effectively spread flat. Points that are in contact with the glass bond electrostatically, but points that are separated from the glass, even by a few microns, do not. Then, when placed into the oven, such raised areas cannot spread flat because closely adjacent areas are already bonded to the slide, and cannot move. After picking up sections from the waterbath, I allow them to stand vertically and drain for no more than 5 minutes, then place them into the drying oven at 70 degrees C. for an hour. I very seldom have any detachment problems with this protocol. Paul M. From lmdee1 <@t> yahoo.com Mon May 21 12:57:55 2012 From: lmdee1 <@t> yahoo.com (Linda) Date: Mon May 21 12:57:59 2012 Subject: [Histonet] Leica CV5030 coverslipper issues Message-ID: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> Hello Everyone, ? ? I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February.? I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. ? I have emailed Leica several times now requesting a new coverslipper.? If I have this many issues at three months what is it going to do in a year? ? The tech service support person has been great with fixing all of the issues. ? This is my reply I received from the President, North America, Jack Kenny- ? "We do not believe that it is appropriate at this point to replace this system. ? We will continute to monitor the situation but not upgrade at this time." ? ? I am not looking for an upgrade I would just like a new coverslipper that works. ? Has anyone else had problems with Leica not replacing defective equipment?? Please let me know.? How did you resolve the issue? ? Thank you in advance, ? ? Linda Dee, BGS, HT(ASCP) From MSHERWOOD <@t> PARTNERS.ORG Mon May 21 12:57:14 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Mon May 21 13:03:44 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> Message-ID: <090FA56107A969459F3941DDD5585C3A1175D266@PHSX10MB10.partners.org> We have a refurbished CV5030 and Autostainer XL and have had no issues with them, or if so, minor ones that did not require contacting anyone. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, ? ? I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February.? I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. ? I have emailed Leica several times now requesting a new coverslipper.? If I have this many issues at three months what is it going to do in a year? ? The tech service support person has been great with fixing all of the issues. ? This is my reply I received from the President, North America, Jack Kenny- ? "We do not believe that it is appropriate at this point to replace this system. ? We will continute to monitor the situation but not upgrade at this time." ? ? I am not looking for an upgrade I would just like a new coverslipper that works. ? Has anyone else had problems with Leica not replacing defective equipment?? Please let me know.? How did you resolve the issue? ? Thank you in advance, ? ? Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From trathborne <@t> somerset-healthcare.com Mon May 21 13:12:27 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon May 21 13:14:30 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <090FA56107A969459F3941DDD5585C3A1175D266@PHSX10MB10.partners.org> References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> <090FA56107A969459F3941DDD5585C3A1175D266@PHSX10MB10.partners.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FE0124@SMCMAIL01.somerset-healthcare.com> Our CV5030 is 7 years old, and although it experiences problems occasionally, it is nothing that we felt it was necessary to have replaced. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Monday, May 21, 2012 1:57 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We have a refurbished CV5030 and Autostainer XL and have had no issues with them, or if so, minor ones that did not require contacting anyone. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, ? ? I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February.? I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. ? I have emailed Leica several times now requesting a new coverslipper.? If I have this many issues at three months what is it going to do in a year? ? The tech service support person has been great with fixing all of the issues. ? This is my reply I received from the President, North America, Jack Kenny- ? "We do not believe that it is appropriate at this point to replace this system. ? We will continute to monitor the situation but not upgrade at this time." ? ? I am not looking for an upgrade I would just like a new coverslipper that works. ? Has anyone else had problems with Leica not replacing defective equipment?? Please let me know.? How did you resolve the issue? ? Thank you in advance, ? ? Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Joyce.Weems <@t> emoryhealthcare.org Mon May 21 13:14:45 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon May 21 13:14:59 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> Message-ID: We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- "We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time." I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From jclark <@t> pcnm.com Mon May 21 13:34:53 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon May 21 13:35:01 2012 Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 24 In-Reply-To: <20120521153817.797808B70A7@mx10.myoutlookonline.com> References: <20120521153817.797808B70A7@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E37867608587@S10MAILD001N3.SH10.lan> We used to have this problem too, till we switched to Tru-Bond slides from Tru-Scientific. Our contact is sara@tru-scientific.com . We also try to dry our slides for 1 hour in the 60 degree oven whenever possible and especially for really fatty tissues, like breast. We haven't had any problems with antigen integrity. I think greater than 60 could cook your tissue and affect things, but if you make sure it doesn't go any higher you should be OK. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 4 Date: Mon, 21 May 2012 09:25:21 -0500 From: "Brendal Finlay" Subject: Re: [Histonet] Help To: 'histonet@lists.utsouthwestern.edu' Message-ID: Content-Type: text/plain; charset="utf-8" Nancy, We've had similar issues with fatty tissue falling off of the slides while performing IHC.?? We use Superfrost + slides which we have found to really hold the tissue well.?? Also, I have learned through reading round on the??Histonet??that air drying doesn't completely remove the water from the middle area of a tissue section.?? For this reason, we no longer air dry at all unless it's a slide that was cut the day before and just happened to be air dried.?? Our protocol changed to cutting the slides and draining them well, then putting them in a 60 C oven for 15 minutes.????Then??the slides are run??down to water on an automated stainer with another 15 minute time in the oven on the stainer.?? A specific instance??when the tissue falls off,??was during antigen retrieval in Trilogy in a pressure cooker.?? If the pressure was manually released, this would cause the Trilogy to boil??and??it would separate the tissue from the slide.?? Ourprotocol changed to 12 minutes in the pressure cooker with Trilogy, then around 8 minutes??to wait for the pressure to release on it's own.?? We would then rinse softly in distilled water to remove the??Trilogy.?? This also seemed to help with the issue. ?? The combination of this??has worked??fairly well for us with some exceptionally stubborn tissue still??attempting to fall off of the slides.????I would love to??hear of other's experiences and??how they resolved this.???? I do wonder about the length of time in your oven.?? I had spoken with one of our Biocare reps about this when we encountered the problem and he felt that longer than 30 minutes in the oven would damage the specimen's IHC integrity.?? Brendal Finlay HT (ASCP) ----Original message----- From: "Cloughley-Gray, Nancy" CloughleyN@rvh.on.ca Date: Fri, 18 May 2012 14:02:35 -0500 To: "'histonet@lists.utsouthwestern.edu'"histonet@lists.utsouthwestern.edu Subject: [Histonet] Help > I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? > I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). > I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. > > Thanking you in advance, > I look forward to your input, > Nancy Cloughley-Gray MLT > From HornHV <@t> archildrens.org Mon May 21 14:00:10 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon May 21 14:00:19 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A1614@EVS1.archildrens.org> We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- "We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time." I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From histotech <@t> imagesbyhopper.com Mon May 21 14:30:59 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon May 21 14:31:12 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A1614@EVS1.archildrens.org> References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719BB4A1614@EVS1.archildrens.org> Message-ID: <447AFFCF-9DDE-4910-8995-33F346C3753F@imagesbyhopper.com> We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken "ears" on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, "Horn, Hazel V" wrote: > We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. > Sent: Monday, May 21, 2012 1:15 PM > To: 'Linda'; histonet@lists.utsouthwestern.edu > Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com > Subject: RE: [Histonet] Leica CV5030 coverslipper issues > > We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda > Sent: Monday, May 21, 2012 1:58 PM > To: histonet@lists.utsouthwestern.edu > Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com > Subject: [Histonet] Leica CV5030 coverslipper issues > > Hello Everyone, > > > I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. > > I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? > > The tech service support person has been great with fixing all of the issues. > > This is my reply I received from the President, North America, Jack Kenny- > > "We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time." > > > I am not looking for an upgrade I would just like a new coverslipper that works. > > Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? > > Thank you in advance, > > > Linda Dee, BGS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sarahjones <@t> ufl.edu Mon May 21 14:46:57 2012 From: sarahjones <@t> ufl.edu (Jones,Sarah A) Date: Mon May 21 14:47:04 2012 Subject: [Histonet] job opportunity University of Florida Message-ID: Hello Histonetters, The Anatomic Pathology Service within the University of Florida Veterinary Diagnostic Laboratories (College of Veterinary Medicine) is recruiting for Laboratory Technician for the Histology Laboratory. The work involves diagnostic veterinary histology and research histology. Full time, 40 hours per week, days, no weekends. The HT (ASCP) is not a requirement for employment. We also do not require the Florida State Laboratory License. A high school diploma and two years of appropriate experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience. Veterinary experience a plus. The University of Florida offers a generous benefits package, including one of the best pension plans in the State. Gainesville is a beautiful college town with large oaks and nearby spring fed rivers for canoeing or kayaking. Housing is very affordable. The job listing can be viewed at: https://jobs.ufl.edu/ Job title: Laboratory Technician job requisition number is 0900939 Sarah A. Jones, HT (ASCP) Histology Lab Manager sarahjones@ufl.edu From adesupo2002 <@t> hotmail.com Mon May 21 22:04:57 2012 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Mon May 21 22:05:03 2012 Subject: [Histonet] P 40 Antibody Message-ID: Hi, I am looking for informations on P 40 Antibody. I want informations like the vendor, procedure e.t.c. Thanks, Adesupo Adesuyi, HTL(ASCP)QIHC NRH, Norman, OK From hcaouette17 <@t> gmail.com Mon May 21 22:12:28 2012 From: hcaouette17 <@t> gmail.com (Heather) Date: Mon May 21 22:12:36 2012 Subject: [Histonet] HTL exam Message-ID: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> Hi, I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. Thanks! -Heather From akbitting <@t> geisinger.edu Tue May 22 04:40:02 2012 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue May 22 04:40:22 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <447AFFCF-9DDE-4910-8995-33F346C3753F@imagesbyhopper.com> References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719BB4A1614@EVS1.archildrens.org> <447AFFCF-9DDE-4910-8995-33F346C3753F@imagesbyhopper.com> Message-ID: <4FBB26B2.2B7F.00C9.1@geisinger.edu> If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to use as a coverslipper. >>> "histotech@imagesbyhopper.com" 5/21/2012 3:30 PM >>> We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken "ears" on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, "Horn, Hazel V" wrote: > We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. > Sent: Monday, May 21, 2012 1:15 PM > To: 'Linda'; histonet@lists.utsouthwestern.edu > Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com > Subject: RE: [Histonet] Leica CV5030 coverslipper issues > > We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda > Sent: Monday, May 21, 2012 1:58 PM > To: histonet@lists.utsouthwestern.edu > Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com > Subject: [Histonet] Leica CV5030 coverslipper issues > > Hello Everyone, > > > I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. > > I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? > > The tech service support person has been great with fixing all of the issues. > > This is my reply I received from the President, North America, Jack Kenny- > > "We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time." > > > I am not looking for an upgrade I would just like a new coverslipper that works. > > Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? > > Thank you in advance, > > > Linda Dee, BGS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From histotech <@t> imagesbyhopper.com Tue May 22 05:34:43 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue May 22 05:34:56 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <4FBB26B2.2B7F.00C9.1@geisinger.edu> References: <1337623075.34932.YahooMailNeo@web162401.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719BB4A1614@EVS1.archildrens.org> <447AFFCF-9DDE-4910-8995-33F346C3753F@imagesbyhopper.com> <4FBB26B2.2B7F.00C9.1@geisinger.edu> Message-ID: <6C952316-950E-4B40-8215-68ADB57C7864@imagesbyhopper.com> We do have the Ventana labeled slides, but coverslip them by hand. The issues we have are with standard, non-labeled slides In fairness to both Leica and the repair company we use, it has been a couple of months since we have had a real problem. Not sure if that is saying a lot, just wanted to be fair. Michelle On May 22, 2012, at 5:40 AM, "Angela Bitting" wrote: > If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to use as a coverslipper. > > >>> "histotech@imagesbyhopper.com" 5/21/2012 3:30 PM >>> > We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken "ears" on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! > > I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! > > Michelle > > On May 21, 2012, at 3:00 PM, "Horn, Hazel V" wrote: > > > We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription > > Anatomic Pathology > > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > > hornhv@archildrens.org > > archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. > > Sent: Monday, May 21, 2012 1:15 PM > > To: 'Linda'; histonet@lists.utsouthwestern.edu > > Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com > > Subject: RE: [Histonet] Leica CV5030 coverslipper issues > > > > We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. > > > > Joyce Weems > > Pathology Manager > > 678-843-7376 Phone > > 678-843-7831 Fax > > joyce.weems@emoryhealthcare.org > > > > > > > > www.saintjosephsatlanta.org > > 5665 Peachtree Dunwoody Road > > Atlanta, GA 30342 > > > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda > > Sent: Monday, May 21, 2012 1:58 PM > > To: histonet@lists.utsouthwestern.edu > > Cc: Kristy.Hanson@leica-microsystems.com; Jack.Kenny@leica-microsystems.com; paul.raimondo@leica-microsystems.com > > Subject: [Histonet] Leica CV5030 coverslipper issues > > > > Hello Everyone, > > > > > > I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. > > > > I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? > > > > The tech service support person has been great with fixing all of the issues. > > > > This is my reply I received from the President, North America, Jack Kenny- > > > > "We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time." > > > > > > I am not looking for an upgrade I would just like a new coverslipper that works. > > > > Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? > > > > Thank you in advance, > > > > > > Linda Dee, BGS, HT(ASCP) > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ________________________________ > > > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and confidential > > and protected from disclosure. If the reader of this message is not the > > intended recipient, or an employee or agent responsible for delivering this > > message to the intended recipient, you are hereby notified that any > > dissemination, distribution or copying of this communication is strictly > > prohibited. If you have received this communication in error, please notify > > us immediately by replying to the message and deleting it from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From mucram11 <@t> comcast.net Tue May 22 07:43:28 2012 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue May 22 07:44:03 2012 Subject: [Histonet] RE: CAP vs. CLIA In-Reply-To: Message-ID: <1529948018.3002.1337690608713.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Thank You Amanda!!! ----- Original Message ----- From: "Amanda Kelley" To: "WILLIAM DESALVO" Cc: "histonet" Sent: Monday, May 21, 2012 9:53:41 AM Subject: Re: [Histonet] RE: CAP vs. CLIA I usually do not comment on the histonet, however this topic is near and dear to my heart. We in Histology are support scientific staff to the Pathologist as much as the Laboratory director is to the Pathologist. Scientific duties, which a pathologist can perform (histological and cytological preparation has been delegated down) to our position. Interpretation of Controls and associated material is often left to us and the Pathologist assistant We determine what the Pathologist can interpret Our expertise directly determines patient outcome, if we are not professional in our decisions then the Pathologist is ineffective in his or hers. The Professional business of running the laboratory is performed by the medical laboratory director, and associate director. ?These are professional positions within the laboratory where a Pathologist has delegated the managerial responsibility to a subordinate. According to CLIA, this position does not exist, only the Pathologist can truly be the Medical laboratory director. Yet in hospitals across the country many Clinical laboratory Scientist's who don't know anything or have limited knowledge of AP are in charge of our AP labs.Their positions are listed as Laboratory Director. This subordinate is the most important person in the lab, yet they too do not turn out results. They are frequently the highest paid in the lab. Their pay is based on the designation of their scientific and managerial background. I believe we can do the same. The business of the pathology lab would be very difficult for the pathologist to handle without this symbiotic relationship of the scientific businessman to the Pathologist. Likewise a Pathologist can not perform their job without the symbiotic relationship to the histotechnologist.Thereby technically, making our jobs indispensable to the Pathologist much like the laboratory director. ?Our problem is history, where many pathologists trained their out of work brother in law to do the work. Many rural areas still run their labs this way. As long as there is a path to become a histotech by hiring whomsoever to do the job. We will always be left behind. So I believe if CLIA and CAP can recognize the laboratory Manager as an important professional position delegated by the Pathologist then we should be afforded the same courtesy. On Sun, May 20, 2012 at 10:37 PM, WILLIAM DESALVO wrote: > > > > > > > > > > > I seemed to have missed something or it might have been all the fresh sea > air I got in Tampa at the FSH, but I do not understand the outrage > expressed towards CLIA and CAP because we are not listed as testing > personnel. I applaud everyone's passion for Histotechnology and the outrage > that we are not allowed to fully participate in the test system model, but > I think we should be directing more of our outrage to the individuals > working in Histotechnology that are not and will not take responsibility to > increase the professionalism of our profession and our own acceptance of > the current state of Histotechnology. > > A TEST SYSTEM is the process that includes pre-analytic, analytic, and > post-analytic steps used to produce a test result or set of results. As > good as we are and as complex parts of the Histotechnology process may be, > Histotechnicians, Histotechnologists and Pathology Assistants do not meet > the standard stated and do not participate in the post-analytic phase, > produce and release patient results. We simply are not able to be > credentialed as is the Medical Technologists and Cytotechnologist. I am not > saying any one laboratory professional group is better than the other, just > that to be considered testing personnel, we must be properly credentialed. > Collectively, we as a discipline, science and group should be working to > upgrade our education requirements and training so that we can become fully > invested partners with the Pathologist. We, not CAP or CLIA, must greatly > increase our professionalism before we can truly be considered competent to > work in the post-analytical phase. I cannot today accept that every working > Histotechnician, Histotechnologist and Pathologist Assistant is able to > produce the "result and release". I am quite sure that every Medical > Technologist and Cytotechnologist is capable and competent to produce and > release a patient result. As things stand today, Histotechnology and all of > us the working in this discipline are a support function to the one person > in our discipline, the Pathologist, that is educated, trained, credentialed > and competent to produce and release a patient result. I also believe there > are many opportunities within our process available now, such as > histochemical staining for organisms, that could allow us to participate in > the post-analytic step. There will be many more as personalized medicine > continues to transform Histotechnology. That said, how can we honestly > promote our participation in the post-analytic phase, when there are far > too many individuals (good, decent and hard working) that work every day, > in every type and complexity of lab, that do not have a formal secondary > education, have participated in defined clinical trials or have completed a > certification exam (required and necessary credentials). Just think how > many practitioners of Histotechnology are out there working today that are > not properly credentialed. Now think if you know of any Medical > Technologist or Cytotechnologist are working that do not have the required > credentials. ?We have many obstacles to increasing the professionalism of > Histotechnology; wide and varied backgrounds, lack of standards, lack of > automation, lack of certification, but I do not think that CAP or CLIA > should be considered one of them. This problem is completely our > responsibility. We first have to demand proper credentials, no exceptions, > no matter the problem, before we can expect other laboratory professionals > to support us in increasing our professionalism and participation in the > healthcare delivery system. As important the need for a robust > accreditation process, healthy discussion must take place before real > change can happen. I suggest we direct our passion and outrage to demand > proper credentials to work in Histotechnology and then demand full > participation in the test system and proper recognition by all laboratory > professionals. > > > William DeSalvo, B.S., HTL(ASCP) > > > > From: JEllin@yumaregional.org > > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > > Date: Thu, 17 May 2012 17:52:44 +0000 > > CC: > > Subject: [Histonet] RE: CAP vs. CLIA > > > > I completely agree with you on this. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > > Sent: Thursday, May 17, 2012 10:46 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > Jesus wrote: > > > > " I think the CAP need to re-evaluate this and re consider what high > complexity testing is, because CLIA defines it not the CAP. ?Remember CAP > enforces CLIA regulation as well as their own. ?" > > > > Certainly the regulations limit the "high complexity" designation to > interpretation of procedure results, but that does not mean a facility does > not need very highly trained and competent technologists to do the > protocols that lead to good interpretation. It simply highlights the > difference between running slides through protocols vs looking at the > result and determining a diagnosis. I'm sure most here will see the > difference. > > > > Remember that CAP is a simply a deemed agency of CLIA - that is, CMS > (Centers for Medicare and Medicaid, which administers the CLIA regulations) > delegates to CAP (and Joint Commission) the authority to accredit > laboratories. CAP cannot make up new regulations, only enforce existing > CLIA regulations. However, the CLIA regulations are by necessity very > general so they can apply to any kind of laboratory operations, current or > future. CAP has the leeway to look at what labs are doing and determine if > the CLIA regulations apply to those tasks. However, CAP must submit their > proposals to CMS/CLIA and CMS/CLIA must pass off on them before they are > implemented. > > > > CAP checklists are far more complex than they were 20 years ago. But the > histo lab is far more complex as well, and regulators (as well as the > public) are looking much more closely at histology because of some major > mistakes that have happened largely due to lack of rigor in testing > validation and implementation. A lot of that has to do with small labs > doing complex testing (interpretation) with methods they were/are not fully > competent to do primarily due to lack of experience and expertise. > > > > While the accreditation process is getting more onerous, it is also > forcing labs to be much more professional in their operations - always a > good thing, I think. > > > > Tim Morken > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. > > Sent: Thursday, May 17, 2012 7:26 AM > > To: 'Jesus Ellin'; 'Horn, Hazel V'; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > Very well said Jesus. ?I agree. > > > > Donna Willis, HT/HTL (ASCP) > > Histology Lab Manager > > Baylor University Medical Center-Dallas > > ph. 214-820-2465 office > > ph. 214-725-6184 mobile > > donna.willis@baylorhealth.edu > > > > > > -----Original Message----- > > From: Jesus Ellin [mailto:JEllin@yumaregional.org] > > Sent: Thursday, May 17, 2012 9:24 AM > > To: 'Horn, Hazel V'; Willis, Donna G.; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: CAP vs. CLIA > > > > I am going to have to go there,, sorry all I know I am going to stir-up > a hornets nest, but here it goes, don't we think that this is done in lue > of the fact that CAP are representing the Pathologist interest and not the > interest of the Technicians. ?Times have changed and the CAP is asking for > more and more from Anatomic Pathology questions every year, not only to > include technical, but also instrumentation (simple and complex), as well > as information systems, predictive markers, Digital Pathology ( a huge > one), etc. ?I think the CAP need to re-evaluate this and re consider what > high complexity testing is, because CLIA defines it not the CAP. ?Remember > CAP enforces CLIA regulation as well as their own. ?I would challenge this. > ?I feel the staff under me do more than turn a wheel, or place tissue in a > mold. ?With Passion comes a need to start to create change, we need this > done. > > > > Jesus Ellin ?HT/PA ASCP, BSBE,MSBE > > Yuma Regional Medical Center > > Anatomic Pathology Supervisor > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > > Sent: Thursday, May 17, 2012 7:05 AM > > To: 'Willis, Donna G.'; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > It was a CAP e alert dated April 2, 2012 > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > > > -----Original Message----- > > From: Willis, Donna G. [mailto:Donna.Willis@baylorhealth.edu] > > Sent: Thursday, May 17, 2012 8:42 AM > > To: Horn, Hazel V; 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: CAP vs. CLIA > > > > I have to say I disagree with this interpretation. ?The commentary in > the 7/11/2011 checklists indicates that regulations apply to "A laboratory > must evaluate and document the competency of all testing personnel for each > test system. A TEST SYSTEM is the process that includes pre-analytic, > analytic, and post-analytic steps used to produce a test result or set of > results." ?To me this includes both histology and pathology office staff. > > > > This is the opinion on myself and our compliance person. ?Hazel can you > tell us where to find the CAP quote. > > > > Thanks, > > > > Donna Willis, HT/HTL (ASCP) > > Histology Lab Manager > > Baylor University Medical Center-Dallas > > ph. 214-820-2465 office > > ph. 214-725-6184 mobile > > donna.willis@baylorhealth.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > > Sent: Thursday, May 17, 2012 7:26 AM > > To: 'Courtney Pierce' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP vs. CLIA > > > > CAP does not consider us testing personnel. How they come by this is a > mystery to me. ? In a recent memo from CAP it describes this: > > > > " Why does CAP require the completion of the "Laboratory Personnel > Evaluation Roster" form and when was this process implemented? > > > > As part of CAP's deemed status with CMS as an accrediting organization, > CMS required CAP to implement a more stringent process to document that > accredited laboratories have appropriately qualified personnel and adequate > documentation of personnel qualifications. The Laboratory Personnel > Evaluation Roster form requires laboratories to confirm that personnel > files contain the information necessary for laboratories to be in > compliance with the CLIA personnel qualification regulations and CAP > Checklist requirements prior to the inspection. It is also used by the > inspection team to assist in the auditing of the records during the > inspection to confirm compliance with the Checklist requirements. The > process of completing the personnel form took effect in August 2009." > > > > And goes on to say: > > "Do I need to list histologists on the Laboratory Personnel Evaluation > Roster? > > > > Typical histologist duties (e.g., fixation, embedding, microtomy, > staining and cover slipping) are not considered testing. Therefore, it is > not necessary to list these personnel on the roster. However, if the > histologist is performing any part of the macroscopic tissue examination > which is considered high complexity testing, it is necessary to list those > personnel on the roster. Such personnel must provide documentation at > minimum of an associate's degree/transcripts or high school diploma or > equivalent for individuals performing grossing at the same laboratory prior > to September 1, 1997." > > > > Hazel Horn > > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hornhv@archildrens.org archildrens.org > > > > > > > > > > 100 YEARS YOUNG! > > JOIN THE PARTY AT > > ach100.org > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Courtney Pierce > > Sent: Wednesday, May 16, 2012 2:27 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] CAP vs. CLIA > > > > > > Can someone help me with the High Complexity Test with CAP vs. CLIA. > > Thanks > > Courtney Pierce > > IHC Specialist > > Quintiles > > Translational R&D - Oncology > > Innovation > > Navigating the new health > > > > 610 Oakmont Lane > > Westmont, IL 60559 > > > > Office: + 630-203-6234 > > courtney.pierce@quintiles.com > > > > clinical | commercial | consulting | capital > > > > > > ********************** ?IMPORTANT--PLEASE READ ?************************ > This electronic message, including its attachments, is COMPANY CONFIDENTIAL > and may contain PROPRIETARY or LEGALLY PRIVILEGED information. ?If you are > not the intended recipient, you are hereby notified that any use, > disclosure, copying, or distribution of this message or any of the > information included in it is unauthorized and strictly prohibited. ?If you > have received this message in error, please immediately notify the sender > by reply e-mail and permanently delete this message and its attachments, > along with any copies thereof. Thank you. > > ************************************************************************ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message > is not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it > from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************** > > This e-mail may contain confidential and/or privileged information. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly forbidden and possibly a violation of federal or state law and > regulations. Baylor Health Care System, its subsidiaries, and affiliates > hereby claim all applicable privileges related to this information. > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message > is not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it > from your computer. > > Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. ?If you are not the intended > recipient(s), you are notified that the dissemination, distribution, or > copying of this message is strictly prohibited. ?If you receive this > message in error, or are not the named recipient(s), please notify the > sender at either the e-mail, fax, address, or telephone number listed above > and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. ?If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. ?If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ?_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akelley1@slu.edu ?DISCLAIMER: ?The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Philip.Gibson <@t> nuth.nhs.uk Tue May 22 08:16:14 2012 From: Philip.Gibson <@t> nuth.nhs.uk (Gibson, Philip) Date: Tue May 22 08:16:20 2012 Subject: [Histonet] Leica CV5030 coverslipper issues Message-ID: <20120522131614.189384487E3@nhs-pd1e-esg101.ad1.nhs.net> We had a bunch of issues with our 5030 coverslipper that have largely been resolved with the following "top tips"!: a) Making sure that the coverslips we load into the machine are not statically-charged (they don't lie flat against each other - the pile feels "springy"). The brand we use only has occasional boxes of 100 coverslips like this, we just use them for hand-mounting instead. b) Scrupulous housekeeping (clean any DPX/glass splinters away regularly) Phil ----------------------------- Mr Philip Gibson Business Manager Directorate of Medicine Newcastle Upon Tyne Hospitals NHS Foundation Trust Tel. 0191 2821511 DECT 21511 ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ******************************************************************************************************************** This email has been processed by SmoothZap - www.smoothwall.net From Jessica <@t> nsh.org Tue May 22 08:17:28 2012 From: Jessica <@t> nsh.org (Jessica Smith) Date: Tue May 22 08:17:35 2012 Subject: [Histonet] NSH 5th Annual Summer Symposium Message-ID: <2B6E973D7D12964F8D8A3598557C5530061E79@NSH-SRVR01.nsh.local> NSH is bringing the 5th Annual Summer Symposium back to Las Vegas, NV! General Sessions and workshops featuring expert speakers will provide you with the tools, advice, and guidance you seek in your professional career. The Summer Symposium is one of the best values for your training dollars in histology education, offering 12 continuing education credits and an exhibit fair for one low price! Current Exhibitors Include: Ameripath/Dermpath Diagnostics, Biocare Medical, Cell Marque Corporation, Leica Microsystems, MediaLab, Inc., Sakura Finetek USA, Simport Scientific, Statlab Medical Products, and Anatech Ltd. Registration Fees: Members: $229 Non-Members: $249 Student Members: $169 HT Readiness Course Only: $99 Registration Brochure: https://s3.goeshow.com/nsh/SS2012/PDF/SSBrochureforWeb.pdf Click here for more details and to register online! https://s3.goeshow.com/nsh/SS2012/ereg419568.cfm?pg=home Have a great week and we hope to see you in Vegas! Jessica Smith Meeting Coordinator/Social Media Specialist National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From mucram11 <@t> comcast.net Tue May 22 08:32:46 2012 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue May 22 08:32:57 2012 Subject: [Histonet] Leica CV5030 coverslipper issues In-Reply-To: <20120522131614.189384487E3@nhs-pd1e-esg101.ad1.nhs.net> Message-ID: <1792296348.4193.1337693566736.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We also have the person taking care of the coverslipper change the brush daily and keep the one in use wet with xylene several times a day.? This seems to expand the life of the dispensing needle and keep it from bending or breaking.? We are doing everything else suggested and the problems are gone.? We have a couple of repair issues however; that were small and quickly fixed. Pam ----- Original Message ----- From: "Philip Gibson" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 22, 2012 8:16:14 AM Subject: Re: [Histonet] Leica CV5030 coverslipper issues We had a bunch of issues with our 5030 coverslipper that have largely been resolved with the following "top tips"!: a) ? ? ?Making sure that the coverslips we load into the machine are not statically-charged ?(they don't lie flat against each other - the pile feels "springy"). ?The brand we use only has occasional boxes of 100 coverslips like this, we just use them for hand-mounting instead. b) ? ? ?Scrupulous housekeeping (clean any DPX/glass splinters away regularly) Phil ? ----------------------------- Mr Philip Gibson Business Manager Directorate of Medicine Newcastle Upon Tyne Hospitals NHS Foundation Trust ? Tel. 0191 2821511 DECT 21511 ? ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ******************************************************************************************************************** This email has been processed by SmoothZap - www.smoothwall.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue May 22 10:08:15 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue May 22 10:08:20 2012 Subject: [Histonet] Monoclonal antibodies from different companies Message-ID: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From chapcl <@t> yahoo.com Tue May 22 10:20:54 2012 From: chapcl <@t> yahoo.com (William) Date: Tue May 22 10:21:13 2012 Subject: [Histonet] Monoclonal antibodies from different companies In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> Message-ID: <7742455B-E19C-4AA3-B030-379DA7299C48@yahoo.com> In general, the antibody itself is the same antibody. Clone types are generally purchased by different companies from the same source. They are usually purchased by the supplying company highly concentrated and then prepared for secondary sale by diluting (even when purchasing concentrating antibodies) and adding buffers and proprietary stabilizers. Antibodies treated in this way can vary substantially from one company to another. Companies also buy cell lines that will retain the same clone number, but will be grown by the ihc supply company in house and prepared as above. These can be better or worse. In general, the best clones are proprietary clones that are sold by the individual company - usually discovered or purchased while partnering with a researcher or educational institution. These can be among the best quality products supplied by an ihc company because the company is putting its best foot forward and wants to protect their exclusive rights. I can provide specifics off-line. Who knows I may work for one of those companies in the future or may have worked for them in the past. Will Chappell, HTL Sent from my iPhone On May 22, 2012, at 11:08 AM, Teri Johnson wrote: > Dear Histonetters, > > If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. > > Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? > > Thanks for your input! > > Teri Johnson, HT(ASCP)QIHC > GNF Histology Lab Manager > Genomics Institute of the Novartis Research Foundation > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aphipps <@t> lmhealth.org Tue May 22 10:21:42 2012 From: aphipps <@t> lmhealth.org (Amanda Phipps) Date: Tue May 22 10:21:46 2012 Subject: [Histonet] HTL exam In-Reply-To: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> Message-ID: Heather, I took the exam last year and used the ASCP guidelines along with the Carson/Hladik (3rd edition) text, the Carson/Hladik Histodeck flash cards, and NSH practice question booklets. I am not sure how old the NSH booklets are because another tech (thankfully!) let me borrow them, but they were extremely helpful. The HistoDeck cards were nice for studying, however the quality of HistoDeck pictures are *much* better than those of the exam. Here is a link to some study materials from NSH http://www.nsh.org/content/certification-exam-study-aids Goodluck!!! Amanda Phipps, HTL (ASCP)cm Histotechnologist Licking Memorial Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather [hcaouette17@gmail.com] Sent: Monday, May 21, 2012 11:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi, I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. Thanks! -Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TGoins <@t> mt.gov Tue May 22 10:22:52 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue May 22 10:23:09 2012 Subject: [Histonet] RE: Monoclonal antibodies from different companies In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> Message-ID: In my experience, identical clones from different companies are not always equivalent. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, May 22, 2012 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue May 22 10:24:53 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue May 22 10:24:50 2012 Subject: [Histonet] RE: Monoclonal antibodies from different companies In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> Message-ID: <8D7C2D242DBD45498006B21122072BF8B50D87C0@MCINFRWEM003.ucsfmedicalcenter.org> Teri, this is an excellent question. Short answer: the same antibody clone from different company's may produce different results. Usually it will be a dilution factor but could be specificity as well if not purified to the same level. There is no way to tell for sure without trying them both. The fact is that some companies make their antibodies and control the entire process. Others repackage antibodies they buy from other companies. Others sell a mix of their own and repackaged. Most likely they won't tell you which is which. Even the clone doesn't make much difference because the companies may each have licensed the same clone from the originator of that clone. Theoretically clones should be the same, but unless they come from the same facility they may not be. One company's production process will be slightly different than another's and so the antibody may produce slightly different results. This can be due to impurities, level of purification, dilution levels (yes they dilute the antibody, even in "concentrate" form!) buffers, etc. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, May 22, 2012 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> cbiolabs.com Tue May 22 10:34:45 2012 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue May 22 10:34:59 2012 Subject: [Histonet] RE: Monoclonal antibodies from different companies In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C9A5392FB@cbiolabs05.CBiolabs.local> Agree with Tresa. It could happen because some companies purchased original clones (not Ig concentrate for further distribution) and screw them. I had such problems occasionally, in particular with now extinct Chemicon. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, May 22, 2012 11:23 AM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Monoclonal antibodies from different companies In my experience, identical clones from different companies are not always equivalent. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, May 22, 2012 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From ASelf <@t> georgetownhospitalsystem.org Tue May 22 12:24:43 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue May 22 12:25:00 2012 Subject: [Histonet] test Message-ID: NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Sherrian.McAnn <@t> va.gov Tue May 22 12:39:39 2012 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue May 22 12:39:57 2012 Subject: [Histonet] (no subject) Message-ID: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a "histotech". I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. From joelleweaver <@t> hotmail.com Tue May 22 12:42:44 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue May 22 12:42:49 2012 Subject: [Histonet] (no subject) In-Reply-To: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> Message-ID: That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 22 May 2012 12:39:39 -0500 > From: Sherrian.McAnn@va.gov > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > I agree and would like to add. This is one scenario that I have seen > many times, where hospitals or wherever will hire histotechs without > certifications . I am thinking that saves them money and they still > have a "histotech". I have seen good histotechs that have no > certification and likewise some bad ones with certification. Lately I > have seen these schools turn out histotechs , it seems with little > encouragement to get certified. If places will hire them without being > certified, there seems little incentive (unless you are self motivated > for more money) to move on up to certification. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue May 22 12:47:42 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue May 22 12:48:13 2012 Subject: [Histonet] P 40 Antibody In-Reply-To: References: Message-ID: Biocare has it. IVD... Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI [adesupo2002@hotmail.com] Sent: Monday, May 21, 2012 11:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P 40 Antibody Hi, I am looking for informations on P 40 Antibody. I want informations like the vendor, procedure e.t.c. Thanks, Adesupo Adesuyi, HTL(ASCP)QIHC NRH, Norman, OK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From evzmedia <@t> yahoo.com Tue May 22 13:00:46 2012 From: evzmedia <@t> yahoo.com (Carl) Date: Tue May 22 13:01:17 2012 Subject: [Histonet] HTL exam In-Reply-To: References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> Message-ID: <64571E32-517E-4003-9098-98B0CD3A4851@yahoo.com> I did the BOR Study guide Second Edition and passed 4 years ago. Carl Ryan Nituda, HTL(ASCP) QIHC Pathology Sciences Medical Sent from my iPhone On May 22, 2012, at 8:21, Amanda Phipps wrote: > Heather, > > I took the exam last year and used the ASCP guidelines along with the Carson/Hladik (3rd edition) text, the Carson/Hladik Histodeck flash cards, and NSH practice question booklets. I am not sure how old the NSH booklets are because another tech (thankfully!) let me borrow them, but they were extremely helpful. > The HistoDeck cards were nice for studying, however the quality of HistoDeck pictures are *much* better than those of the exam. > > Here is a link to some study materials from NSH > > http://www.nsh.org/content/certification-exam-study-aids > > Goodluck!!! > > Amanda Phipps, HTL (ASCP)cm > Histotechnologist > Licking Memorial Hospital > > > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather [hcaouette17@gmail.com] > Sent: Monday, May 21, 2012 11:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HTL exam > > Hi, > I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. > Thanks! > -Heather > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue May 22 13:01:47 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue May 22 13:01:58 2012 Subject: [Histonet] CD133 on Ventana Benchmark Ultra Message-ID: <002401cd3844$f4fd8780$def89680$@gmx.at> Hi histonetters! Can anyone provide me with a successful protocol for CD133/1 from Miltenyi on the Ventana Benchmark Ultra? Today I searched the web for this antibody. It seems like this company has the exclusive right for selling it.?. Their website contents also a forum. There are some posts of unsatisfied costumers with problems to get the IHC working on FFPE tissue. Any experiences? Thanks in advance Gudrun From newhiretracking <@t> hotmail.com Tue May 22 14:46:55 2012 From: newhiretracking <@t> hotmail.com (Heather Marlatt) Date: Tue May 22 14:46:58 2012 Subject: [Histonet] Reagent containers??? Message-ID: Does anyone have reagent containers and slide baskets for a Shandon LTD Varistain XY Slide stainer that they would be willing to get rid of? Feel free to reply to my personal email addy Thanks Heather From tony.henwood <@t> health.nsw.gov.au Tue May 22 19:48:00 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue May 22 19:48:13 2012 Subject: [Histonet] RE: Monoclonal antibodies from different companies In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A4ACF3@xmdb02.nch.kids> Hi Teri If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, IT IS THE SAME. There is no any benefit in buying the antibody from Company B to try, since it is a duplication of effort and cost Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, 23 May 2012 1:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotech <@t> imagesbyhopper.com Tue May 22 19:50:12 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue May 22 19:50:26 2012 Subject: [Histonet] Cytology Staining In-Reply-To: <3340FC2AE9CFEE4E9D001D077700C6A2253ED944@NT106.info.sys> References: <1337253739.33117.YahooMailNeo@web161705.mail.bf1.yahoo.com> <3340FC2AE9CFEE4E9D001D077700C6A2253ED944@NT106.info.sys> Message-ID: <8DCF27BB-462F-416A-A89C-D67E7E9083FC@imagesbyhopper.com> Is Cyto prep considered a high complexity task? On May 17, 2012, at 12:59 PM, "Yang, Mari" wrote: > Sheila, > > Maybe the inspector was referring to the prep? Many cytology labs use automated staining. If you find out any information, I'd love to know. > > Thanks, > Mari > > Mari Yang, MHA, CT(ASCP)CMHTLCM > Cytology Supervisor > Tel: 760.773.2009 > > P Save a tree, please don't print this e-mail unless you really need to. > > Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Thursday, May 17, 2012 4:22 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cytology Staining > > Hello all. During an inspection yesterday, the inspector made a brief, vague > reference to cytology staining now being deemed "high complexity". Does > anyone have information and/or a reference for this? > I'd appreciate any information anyone could provide. > Thanks a bunch. > > Sheila Haas > Laboratory Manager > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood <@t> health.nsw.gov.au Tue May 22 19:58:50 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue May 22 19:59:01 2012 Subject: [Histonet] RE: Monoclonal antibodies from different companies In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A4ACF3@xmdb02.nch.kids> References: <9F3CFEE76E51B64991C7485270890B4009F42DB5@EX5.lj.gnf.org> <6D6BD1DE8A5571489398B392A38A715760A4ACF3@xmdb02.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A4AD67@xmdb02.nch.kids> Gee I do live in an ideal world, looking through rose coloured glasses - definitely affects my interpretation of an H&E stain! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, 23 May 2012 10:48 AM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Monoclonal antibodies from different companies Hi Teri If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, IT IS THE SAME. There is no any benefit in buying the antibody from Company B to try, since it is a duplication of effort and cost Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, 23 May 2012 1:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Tue May 22 23:28:51 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Tue May 22 23:28:59 2012 Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 26 In-Reply-To: <4fbb8a31.658bb60a.6804.ffffc983SMTPIN_ADDED@mx.google.com> References: <4fbb8a31.658bb60a.6804.ffffc983SMTPIN_ADDED@mx.google.com> Message-ID: >> I'm a Histotechnologist working in the Regional Hospital in Barrie, > ON Canada. We are using the Ventana Ultra for our Immunohistochemistry > (IHC). Since the end of February, we have been having issues with some > tissues lifting off our positive (marked with +) charged slides. It > seems to be mostly with the fatty and/or larger sections. We now dry > our slides for one hour at room temperature (R.T.) and an additional > hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have > tried 2 different types of + slides and will be trying another type of > charged slide (from Newcomer this time) I was wondering if anyone has > any other suggestions? >> I also have another question regarding a QC (quality control) issue. > We use a multi-tissue control that is applied to the top of all our > test slides for IHC. One of our paths commented that there is some > positive staining in the smooth muscle nuclei of thenormal bowel when > we are testing for Progesterone (PR). We are using a Heat Induce > Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary > buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 > minutes with PR clone 1E2. (Ventana instrumentation provides > pre-diluted antibodies and the user adjusts the concentration of the > antibody by adjusting the time the primary antibody is incubated with > the tissue). >> I am concerned about the implications of this staining and I have > not been able to find a reference to this kind of unusual staining > pattern. The bowel tissue that we are using as QC is from a 62 year > old female patient. I was wondering if anyone has had any experience > with this kind of staining and /or any references that I could use. >> >> Thanking you in advance, >> I look forward to your input, >> Nancy Cloughley-Gray MLT Nancy, Your problem is very common, due to fatty tissue is difficult to process. Here's my suggestion if you want to salvage your fatty tissue blocks (presumably the fatty tissues are well fixed); 1) melt down the fatty tissue block & re-process 2) cut & put on charged slides (Fisher Scientific "Ultra Stick" charged slides work the best) 3) bake slide for 30 min @ 60c 4) do antigen retrieval with Biocare's Diva for 3 min in Pascal or Biocare pressure cooker (very important; do NOT use Triology & Cell Marque pressure cooker). 5) do your ihc as usual Three important elements; (1) well process tissue, (2) Biocare Diva AR buffer, & (3) Pascal pressure cooker. Keep us posted what's your outcome. Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - Pathology (histology & IPOX) madeleine_h@elcaminohospital.org From Melissa.Kuhnla <@t> chsli.org Wed May 23 06:23:09 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed May 23 06:23:46 2012 Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 26 In-Reply-To: References: <4fbb8a31.658bb60a.6804.ffffc983SMTPIN_ADDED@mx.google.com> Message-ID: Hello, I also use Ventana Ultra instruments. We have had our air share of tissue adhesion issues as well. We currently have most of it under control by doing the following: 1. We switched from standard plus slides to Fisherbrand Excel Adhesion slides (cat #22-034-985). I believe they are meant for automated staining, solutions of varying temp and pH, etc. We did see an improvement!! 2. We at all time handle our slides with gloves on. We dip and dry controls upside-down. I was very clear to us that dipping and handling the slide while cutting the control section altered the charge of the rest of the slide. As a rule of thumb...if we need to repeat things due to tissue loss, we run it on a slide by itself and run a separate control. If a focus of tumor is very small or tissue is minimal to begin with, a pathologist may request separate controls right from the start. 3. We also altered some of our processing schedules. At first we were loosing a lot of small biopsies and FNAs. We shortened our processing and now they adhere fine!! 4. We discontinued the use of recycled Xylene in the processor as well. BTW, We routinely cut at four microns, dry vertical for 15 minutes and bake at 60 for 90-120 minutes We have this issue under such a microscope here that we know it will be impossible to get rid of this all together. Breast cases (and sometimes skin) remain to be the only culprits. These tissues just by nature may always be a problem. We now are contemplating running batched controls for ER/PR cases so all those cases are on their own slides. Hope this helps. Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Wednesday, May 23, 2012 12:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 26 >> I'm a Histotechnologist working in the Regional Hospital in Barrie, > ON Canada. We are using the Ventana Ultra for our Immunohistochemistry > (IHC). Since the end of February, we have been having issues with some > tissues lifting off our positive (marked with +) charged slides. It > seems to be mostly with the fatty and/or larger sections. We now dry > our slides for one hour at room temperature (R.T.) and an additional > hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have > tried 2 different types of + slides and will be trying another type of > charged slide (from Newcomer this time) I was wondering if anyone has > any other suggestions? >> I also have another question regarding a QC (quality control) issue. > We use a multi-tissue control that is applied to the top of all our > test slides for IHC. One of our paths commented that there is some > positive staining in the smooth muscle nuclei of thenormal bowel when > we are testing for Progesterone (PR). We are using a Heat Induce > Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary > buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 > minutes with PR clone 1E2. (Ventana instrumentation provides > pre-diluted antibodies and the user adjusts the concentration of the > antibody by adjusting the time the primary antibody is incubated with > the tissue). >> I am concerned about the implications of this staining and I have > not been able to find a reference to this kind of unusual staining > pattern. The bowel tissue that we are using as QC is from a 62 year > old female patient. I was wondering if anyone has had any experience > with this kind of staining and /or any references that I could use. >> >> Thanking you in advance, >> I look forward to your input, >> Nancy Cloughley-Gray MLT Nancy, Your problem is very common, due to fatty tissue is difficult to process. Here's my suggestion if you want to salvage your fatty tissue blocks (presumably the fatty tissues are well fixed); 1) melt down the fatty tissue block & re-process 2) cut & put on charged slides (Fisher Scientific "Ultra Stick" charged slides work the best) 3) bake slide for 30 min @ 60c 4) do antigen retrieval with Biocare's Diva for 3 min in Pascal or Biocare pressure cooker (very important; do NOT use Triology & Cell Marque pressure cooker). 5) do your ihc as usual Three important elements; (1) well process tissue, (2) Biocare Diva AR buffer, & (3) Pascal pressure cooker. Keep us posted what's your outcome. Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - Pathology (histology & IPOX) madeleine_h@elcaminohospital.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From kiran_g <@t> sbcglobal.net Wed May 23 09:25:45 2012 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Wed May 23 09:26:03 2012 Subject: [Histonet] Excellent opportunity: Histology Manager in Berkeley, CA Message-ID: <1337783145.52650.YahooMailClassic@web180101.mail.gq1.yahoo.com> Regional Laboratory Section Manager ? Histology Company Name: Kaiser Permanente Approximate Salary: Not Specified Location: Berkeley, California Country: United States Industry: Management Position type: Full Time Experience level: > 5 years Education level: Bachelor's Degree ID 714 Short Description: Regional Laboratory Section Manager, Histology - Kaiser Permanente in Berkeley, California Long Description: Success is measured one step at a time I hold myself to the highest standards. And believe in my ability to make a difference?one day at a time. As an employee of Kaiser Permanente, I have the freedom to put these beliefs into practice. Here, I have the resources and support I need to realize my potential. If you believe professional empowerment leads to personal satisfaction, this is the place to put your beliefs into practice. We have the following full-time position in Berkeley, California. Regional Laboratory Section Manager ? Histology In this role, you will direct and control the day-to-day Regional Laboratory Histology department operations including all personnel activities, technical oversight, and work flow. You will participate in the design of the Regional Laboratory and Northern California Region integrated laboratory quality system; design and implement effective risk control processes; and will ensure compliance with regulatory and accreditation agencies? rules and regulations. You will also lead in the research of new and/or improved test development methodologies by performing experimental testing procedures; validating effectiveness/feasibility for implementation; cost of procedures; preparing and submitting recommendations for change to laboratory management and other stakeholders (such as Chiefs of Pathologists). You will also participate in department, inter-department, inter-facility, and inter-regional level projects which help the regional laboratory achieve its goal of providing quality service and client support in a cost effective manner. In addition, you will serve as a primary liaison to RILIS/ITS for ongoing and new issues. Qualifications include: ? At least five years of experience in a high-volume histology laboratory ? Previous supervisory/managerial experience (usually three years) ? A bachelor?s degree in biological sciences or a related field; a master?s degree in science is preferred ? Additional courses in business administration and/or management preferred ? Certification by the American Society for Clinical Pathologists ? IHC laboratory operations experience preferred ? IHC method optimization/validation experience and IHC instrumentation experience preferred ? The ability to work in a Labor/Management Partnership environment We offer a highly competitive salary and an exceptional benefits package. For immediate consideration, please e-mail your resume to Charlene.Schaeffer@kp.org or visit http://jobs.kp.org for complete qualifications and job submission details, referencing job number 133637. Principals only. EOE/AA Employer. If you would like to hear the Kaiser Permanente story as told by our employees, watch the videos at kp.org/jobs/video. Follow us on twitter.com/KPCareers or visit the KP Careers tab on facebook.com/KPThrive. This position supports Kaiser Permanente?s code of conduct and compliance by adhering to all laws and regulations, accreditation and licensure requirements, and internal policies and procedures. jobs.kp.org KAISER PERMANENTE Contact: Charlene.Schaeffer@kp.org ? From tpodawiltz <@t> lrgh.org Wed May 23 09:45:52 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed May 23 09:46:03 2012 Subject: [Histonet] (no subject) In-Reply-To: References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> The last person I hired for a tech position, was just finishing her on-line program. I helped her complete that and then she was given two years to get her certification. This was all part of her hiring agreement, so she knew this coming in and completely understood that failing to achieve either one would affect her employment. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 22, 2012 1:43 PM To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 22 May 2012 12:39:39 -0500 > From: Sherrian.McAnn@va.gov > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > I agree and would like to add. This is one scenario that I have seen > many times, where hospitals or wherever will hire histotechs without > certifications . I am thinking that saves them money and they still > have a "histotech". I have seen good histotechs that have no > certification and likewise some bad ones with certification. Lately I > have seen these schools turn out histotechs , it seems with little > encouragement to get certified. If places will hire them without being > certified, there seems little incentive (unless you are self motivated > for more money) to move on up to certification. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Stacy_McLaughlin <@t> cooley-dickinson.org Wed May 23 09:51:12 2012 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Wed May 23 09:56:07 2012 Subject: [Histonet] CAP new reagent lot verification Message-ID: Hello Histoland, How are you handling ANP.22760 New Reagent Lot Verification: What do you use for "an appropriate panel of control tissues...made on slides cut from the same control block" Do you have multi-tissue (panel) blocks for each antibody, do you have one multi-tissue block that is good for all of your antibodies? We are trying to make multi-tissue blocks for each antibody, trying to use some for multiple antibodies (i.e. melanoma panels, etc.) Any better suggestions? Any advice you can offer an overwhelmed tech would be greatly appreciated! J Thanks Stacy From arlange <@t> medicine.nevada.edu Wed May 23 10:03:17 2012 From: arlange <@t> medicine.nevada.edu (Alicia R. Lange) Date: Wed May 23 10:03:26 2012 Subject: [Histonet] RESTAINING WITH KI67 Message-ID: <9809822F80B1414C90EB4438713C896801345590@MEDX.medicine.nevada.edu> Hello Histoland! Has anyone restained tunel slides with ki67? Since tunel stains apoptosis and ki67 proliferation, I was just going to restain with ki67. I wanted to get some input before I did this. Thanks! Alicia Lange Laboratory Supervisor University of Nevada, Reno Pathology Department arlange@medicine.nevada.edu From Margaret.Perry <@t> sdstate.edu Wed May 23 10:31:41 2012 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed May 23 10:31:47 2012 Subject: [Histonet] PVK stain Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE032365@SDSU-EX03.jacks.local> We are in need of some help trouble shooting this stain. Is it normal for the tissue nuclei to stain red? If not what are we doing wrong? The Chlamydia are staining OK. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From joelleweaver <@t> hotmail.com Wed May 23 11:41:10 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 23 11:41:19 2012 Subject: [Histonet] (no subject) In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov>, , <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> Message-ID: TomI compliment your approach, and I personally really like to see when employers do this. I think it provides opportunity, yet incentive. Two years seems especially reasonable. Most places that I have known who use this method for new-grad hires, have allowed 1 year. It is even better when they provide additional training and support to the person as they are preparing. Having been involved in an online program and clinical curricula in the past, I feel from my observations that continuity with work in the environment is essential for most in connecting the theory with execution. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tpodawiltz@lrgh.org > To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > Date: Wed, 23 May 2012 10:45:52 -0400 > Subject: RE: [Histonet] (no subject) > > The last person I hired for a tech position, was just finishing her on-line program. I helped her complete that and then she was given two years to get her certification. This was all part of her hiring agreement, so she knew this coming in and completely understood that failing to achieve either one would affect her employment. > > > > Tom Podawiltz HT (ASCP) > Histology Section Head/Laboratory Safety Officer. > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Tuesday, May 22, 2012 1:43 PM > To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) > > > That seems to be the unfortunate situation at this time... > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > Date: Tue, 22 May 2012 12:39:39 -0500 > > From: Sherrian.McAnn@va.gov > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] (no subject) > > > > I agree and would like to add. This is one scenario that I have seen > > many times, where hospitals or wherever will hire histotechs without > > certifications . I am thinking that saves them money and they still > > have a "histotech". I have seen good histotechs that have no > > certification and likewise some bad ones with certification. Lately I > > have seen these schools turn out histotechs , it seems with little > > encouragement to get certified. If places will hire them without being > > certified, there seems little incentive (unless you are self motivated > > for more money) to move on up to certification. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > From Courtney.Pierce <@t> quintiles.com Wed May 23 11:57:08 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Wed May 23 11:57:17 2012 Subject: [Histonet] Antibody titer Message-ID: When you titer a new antibody you go one below and one above the recommend dilution. If you find that the one above the dilution works do you go then out two more to make sure they don't work? This is a question in our lab right now. Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From TNMayer <@t> mdanderson.org Wed May 23 12:49:17 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed May 23 12:49:45 2012 Subject: [Histonet] RE: HTL exam Message-ID: We used to have our students take the BOC just before graduation in the summer, but found that the stress of finals coupled with the BOC was a bit much. Especially for those who worked. We do require that the students sign up for the exam (we do this in class as a group) and advise them to take it within about a month of graduation. Some of the students request extra tutoring for the exam and we try to accommodate that as well. Employers that are changing their requirements want registered or eligible; or completion of an accredited program, usually. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 2 Date: Tue, 22 May 2012 12:39:39 -0500 From: "McAnn, Sherrian" Subject: [Histonet] (no subject) To: Message-ID: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> Content-Type: text/plain; charset="us-ascii" I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a "histotech". I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. ------------------------------ Message: 3 Date: Tue, 22 May 2012 17:42:44 +0000 From: joelle weaver Subject: RE: [Histonet] (no subject) To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 22 May 2012 12:39:39 -0500 > From: Sherrian.McAnn@va.gov > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > I agree and would like to add. This is one scenario that I have seen > many times, where hospitals or wherever will hire histotechs without > certifications . I am thinking that saves them money and they still > have a "histotech". I have seen good histotechs that have no > certification and likewise some bad ones with certification. Lately I > have seen these schools turn out histotechs , it seems with little > encouragement to get certified. If places will hire them without > being certified, there seems little incentive (unless you are self > motivated for more money) to move on up to certification. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************* From amosbrooks <@t> gmail.com Wed May 23 13:19:34 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 23 13:19:42 2012 Subject: [Histonet] Re: Histonet Digest, Vol 102, Issue 28 In-Reply-To: <4fbd17bb.a4183c0a.593b.ffffdfedSMTPIN_ADDED@mx.google.com> References: <4fbd17bb.a4183c0a.593b.ffffdfedSMTPIN_ADDED@mx.google.com> Message-ID: Hi, I have done this. It works quite well. It would probably be best to use an alkaline phosphatase detection since the brilliant red of the fast red precipitate is more distinguishable from the brown DAB. (I am assuming you used HRP-DAB detection of the Tunel.) You needn't worry about the left over HRP since you'll be doing an antigen retrieval step which should wash them all out. Amos On Wed, May 23, 2012 at 1:00 PM, wrote: > Message: 16 > Date: Wed, 23 May 2012 08:03:17 -0700 > From: "Alicia R. Lange" > Subject: [Histonet] RESTAINING WITH KI67 > To: > Message-ID: > <9809822F80B1414C90EB4438713C896801345590@MEDX.medicine.nevada.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello Histoland! > > > > Has anyone restained tunel slides with ki67? Since tunel stains > apoptosis and ki67 proliferation, I was just going to restain with ki67. > I wanted to get some input before I did this. Thanks! > > > > Alicia Lange > From Rcartun <@t> harthosp.org Wed May 23 13:25:16 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed May 23 13:27:30 2012 Subject: [Histonet] Antibody titer In-Reply-To: References: Message-ID: <4FBCF344.7400.0077.1@harthosp.org> Yes, I always want to see the immunoreactivity "drop-off" (or even disappear) with a higher dilution(s). Otherwise, you might be wasting antibody by using a dilution that is too concentrated. Also, be careful with the recommended dilution. That dilution is only applicable if you are using the exact same staining conditions. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Courtney Pierce 5/23/2012 12:57 PM >>> When you titer a new antibody you go one below and one above the recommend dilution. If you find that the one above the dilution works do you go then out two more to make sure they don't work? This is a question in our lab right now. Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBUSTAMANTE <@t> cvm.tamu.edu Wed May 23 13:41:31 2012 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Wed May 23 13:41:35 2012 Subject: [Histonet] Microwave verification Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. From tajibade <@t> echd.org Wed May 23 14:33:33 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Wed May 23 14:33:45 2012 Subject: [Histonet] RE: Microwave verification In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> Message-ID: Can you please give more details about this question? Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel: 432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, May 23, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave verification Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From marlmuel <@t> ethz.ch Wed May 23 15:24:29 2012 From: marlmuel <@t> ethz.ch (=?ISO-8859-1?Q?Marlen_M=FCller?=) Date: Wed May 23 15:28:02 2012 Subject: [Histonet] RNA extraction and amplification of LCM rice grain tissue In-Reply-To: References: <4fbd17bb.a4183c0a.593b.ffffdfedSMTPIN_ADDED@mx.google.com> Message-ID: <4FBD477D.6070509@ethz.ch> Dear HistoNet Community, Currently we are working on a procedure to dissect the grain of the rice plant via a PALM Laser Microdisection System. According to published work in the barley grain (Schiebold et al 2011) 2 kits for RNA extraction, purification and amplification were used (for mRNA extraction "Dynabeads mRNA DIRECT MicroKit" or for total RNA extraction"RNeasy MicroKit" and for RNA amplification "C&E Version ExpressArt mRNA amplification Nanokit"). We will use the extracted and amplified RNA then for high-throughput RNA Sequencing. Do you have any experience with RNA amplification for very small amount of tissues? Would you use the classical trizol method or should I rely on a commercial kit for extraction? Thanks alot for sharing your knowledge! Best regards, Marlen -- ETH Z?rich *Marlen M?ller* PhD Student Group of Plantbiotechnology Universit?tstrasse 2 8092 Z?rich Switzerland From Joyce.Weems <@t> emoryhealthcare.org Wed May 23 15:26:32 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed May 23 15:28:04 2012 Subject: [Histonet] RE: Microwave verification In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> Message-ID: Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ? Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: ?Reproducibility? is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ? Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ? Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ? Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, May 23, 2012 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave verification Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From POWELL_SA <@t> mercer.edu Wed May 23 15:31:01 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed May 23 15:31:15 2012 Subject: [Histonet] old equipment for parts Message-ID: <9BF995BC0E47744E9673A41486E24EE24B373A6352@MERCERMAIL.MercerU.local> I have an old Tissue Tek III cryo console that only gets luke cool and a ThermoFisher slide dryer that died on me. If anyone wants these for parts or boat anchors you can have them, you pay shipping. I hate to trash them if the parts can be recycled. sp Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From Joyce.Weems <@t> emoryhealthcare.org Wed May 23 15:33:44 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed May 23 15:33:53 2012 Subject: [Histonet] RE: Microwave verification In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> Message-ID: Ok - attachment didn't work. Whoever wants the procedure, just let me know it is ok to send separately! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 23, 2012 4:27 PM To: 'Bustamante, Lin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microwave verification Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ? Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: ?Reproducibility? is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ? Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ? Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ? Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, May 23, 2012 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave verification Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From amber.mckenzie <@t> gastrodocs.net Wed May 23 16:07:55 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed May 23 16:05:56 2012 Subject: [Histonet] Grossing hours In-Reply-To: <64571E32-517E-4003-9098-98B0CD3A4851@yahoo.com> References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> <64571E32-517E-4003-9098-98B0CD3A4851@yahoo.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC11688DDA@JERRY.Gia.com> How many hours of Science does a person need to be qualified to Gross (under CAP and CLIA)? From LBUSTAMANTE <@t> cvm.tamu.edu Wed May 23 16:28:38 2012 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Wed May 23 16:28:42 2012 Subject: [Histonet] RE: Microwave verification In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu> , Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB3497E@CVMMB02.cvm.tamu.edu> Thank you so much. You are all great. Lin :-) ________________________________________ From: Weems, Joyce K. [Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, May 23, 2012 3:33 PM To: Weems, Joyce K.; Bustamante, Lin; histonet@lists.utsouthwestern.edu Subject: RE: Microwave verification Ok - attachment didn't work. Whoever wants the procedure, just let me know it is ok to send separately! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 23, 2012 4:27 PM To: 'Bustamante, Lin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microwave verification Someone else asked about this as well.. hope the attachment of our very simple instructions is ok to send. Sor far this has worked for us...j Here is CAP checklist: ANP.27170 Microwave Usage Phase I Microwave devices are used in accordance with manufacturer's instructions. NOTE: Microwave devices should be used in accordance with manufacturer's instructions, unless CAP requirements are more stringent. Evidence of Compliance: ? Written procedure for microwave usage ANP.28290 Microwave Monitoring Phase I Microwave devices are at least annually monitored for reproducibility. NOTE: ?Reproducibility? is defined as consistency in diagnostic quality obtained from microwave equipment and procedures. For some devices, reproducibility may be evaluated by monitoring the temperatures of identical samples after microwave processing. For those microwave devices (particularly those incorporated into histology processing equipment) that use temperature-independent methods to evaluate reproducibility, the laboratory should have a written procedure for monitoring reproducibility that follows instrument manufacturer's instructions. Information on such procedures is given in the reference to this checklist requirement (see below). The microwave device should be tested for radiation leakage if there is visible damage to the device. Evidence of Compliance: ? Written procedure for monitoring the diagnostic quality of specimens processed using microwaves ANP.28860 Microwave Container Venting Phase I All containers used in microwave devices are vented. NOTE: Venting of containers is necessary so that processing occurs at atmospheric pressure, to prevent explosion. For procedures using pressure above that of the atmosphere, specialized containers must be used, with strict adherence to manufacturer instructions. Evidence of Compliance: ? Written procedure for the use of appropriately vented containers ANP.29430 Microwave Venting Phase I Microwave devices are properly vented. NOTE: Microwave devices should be placed in an appropriate ventilation hood to contain airborne chemical contaminants and potentially infectious agents. Before operation of the microwave device, 36 of 43 Emory/Saint Josephs, Inc Main Laboratory Anatomic Pathology Checklist 01.04.2012 flammable and corrosive reagents should be removed from the hood, to prevent fire or chemical damage to the electronic components of the device. Microwave devices used outside a fume hood should have an integral fume extractor that is certified by the manufacturer for use in a clinical laboratory. The effectiveness of ventilation should be monitored at least annually. This checklist requirement does not apply if only non-hazardous reagents (and non-infectious specimens) are used in the device (e.g. water, certain biological stains, paraffin sections). The laboratory should consult the MSDS sheets received with reagents and stains to assist in determining proper handling requirements and safe use. This checklist item does not apply to microwave devices that are designed by the manufacturer to operate without venting. Evidence of Compliance: ? Records of annual evaluation of ventilation effectiveness Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Wednesday, May 23, 2012 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave verification Does anyone knows a protocol for Microwave verification? (CAP requirement). Thank you. Lin Bustamante Central Texas Gastrointestinal Clinic Histology laboratory. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lpwenk <@t> sbcglobal.net Thu May 24 04:43:32 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 24 04:43:41 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov>, , <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> Message-ID: <29496B3FDE874BA18B4B3A7A07111F37@HP2010> I'd like to wade into this discuss with a couple of comments: LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": Yes, I'm still hearing about places like this. When I talk with the supervisors, it's because the lab wants the person doing the "histotech" job, but they only want to pay them at "lab assistant" wages. Plus, once they get the people trained as "histotechs", the employees can't go elsewhere, because the other labs only want certified histotech, and these people can't get certified as they don't have the associate degree and minimum 12 hours of biology and chemistry combined as required to take the ASCP HT exam. So these people end up having to stay there. (Personally, I think is very unfair to the employees they hire.) LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: Even though the High School route was dropped as of Jan 1, 2005 (over 7 years ago), I still get emails from labs that want to hire one of my students, but their job description says high school diploma. I usually call these places up, and the histology supervisor had no idea the ASCP HT high school route was dropped. "Someone" should have told them. Even though it was in every "NSH in Action" for the 5 years previous (that's now over 12 years ago), in some ASCP publications each year for the 5 years previous, and on both the NSH and ASCP webpage for the 5 years previous, well, since they aren't NSH or ASCP members, well, "someone" still should have contacted them directly and let them know. Sigh. I've had employees call that they were hired after the 2005 deadline, with the job description of high school graduate requirement, and were told they had 2 years to get the experience required, and then they had 1 additional year in which to take and pass the HT exam. And when they went to sign up to take the HT exam, they discovered that the HT exam requirements had dropped the high school route and now the on-the-job (OJT) requires the associate degree/60 credit hours with 12 credits of bio/chem, which of course they don't have. They tell me that their histology supervisor says they are going to fire them, because they can't take the ASCP HT Exam. I end up talking with the supervisor, and advise them to talk with their HR and Legal departments, as they are the ones who advertised the high school requirement, and they are the ones who hired this person without the needed education. And I suggest they help with person complete an on-line NAACLS HT program, several of which will take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, but for most lab training programs.) NAACLS has a long list of standards for programs to follow. (Think CAP checklist.) Standard 14 G has a statement "The granting of the degree or certificate must not be contingent upon the student's passing any type of external certification or licensure examination." (Explanation: Not all HT programs end in an associate degree. The certificate refers to a certificate of completion of a program. My program, for example, is hospital-based. Some students already have their degree before they start my program. Some have all the college credits except for the ones they are earning while completing the internship, then they earn their degree from the college when they complete the internship and get the grade for those last credit hours. The hospital doesn't grant the degree, the college does. The hospital program grants a certificate of completion of the program, which is acceptable to NAACLS, ASCP, and employers.) As NAACLS accredited HT or HTL programs, we can encourage our students to take the HT/HTL exam upon completion of the program. We can do review sessions with them. We can remind them of the deadlines to sign up. We can help them sign up if they are having problems. We can let them know that labs in our area expect people to be certified. We can let them know that they can sign up while still in the program (couple of months before graduation), and they can, before they graduate, pick a date to take the exam after graduation. We can tell them that these dates to take the exams can be put on their resume, on the application, and that they can inform the supervisor during the interview that they are already signed up to take the HT/HTL exam. But we can NOT "make" the student take the exam. Completion of the program cannot be contingent upon taking or passing the HT/HTL exam (or getting state licensure). The program could lose NAACLS accreditation if we force the student to take the HT/HTL/state licensure exam, or withhold their degree or certificate until they do take/pass the HT/HTL exam/become state licensed. Thanks for "listening". Peggy A. Wenk, HTL(ASCP)SLS Program Director, Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are my own, and do not represent Beaumont Hospital.) -----Original Message----- From: joelle weaver Sent: Wednesday, May 23, 2012 12:41 PM To: tpodawiltz@lrgh.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) TomI compliment your approach, and I personally really like to see when employers do this. I think it provides opportunity, yet incentive. Two years seems especially reasonable. Most places that I have known who use this method for new-grad hires, have allowed 1 year. It is even better when they provide additional training and support to the person as they are preparing. Having been involved in an online program and clinical curricula in the past, I feel from my observations that continuity with work in the environment is essential for most in connecting the theory with execution. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tpodawiltz@lrgh.org > To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > histonet@lists.utsouthwestern.edu > Date: Wed, 23 May 2012 10:45:52 -0400 > Subject: RE: [Histonet] (no subject) > > The last person I hired for a tech position, was just finishing her > on-line program. I helped her complete that and then she was given two > years to get her certification. This was all part of her hiring agreement, > so she knew this coming in and completely understood that failing to > achieve either one would affect her employment. > > > > Tom Podawiltz HT (ASCP) > Histology Section Head/Laboratory Safety Officer. > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Tuesday, May 22, 2012 1:43 PM > To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) > > > That seems to be the unfortunate situation at this time... > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > Date: Tue, 22 May 2012 12:39:39 -0500 > > From: Sherrian.McAnn@va.gov > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] (no subject) > > > > I agree and would like to add. This is one scenario that I have seen > > many times, where hospitals or wherever will hire histotechs without > > certifications . I am thinking that saves them money and they still > > have a "histotech". I have seen good histotechs that have no > > certification and likewise some bad ones with certification. Lately I > > have seen these schools turn out histotechs , it seems with little > > encouragement to get certified. If places will hire them without being > > certified, there seems little incentive (unless you are self motivated > > for more money) to move on up to certification. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication > in error, please notify the sender by return e-mail and delete this > message and any attachments from your computer. Any views or opinions > expressed are solely those of the author and do not necessarily represent > those of LRGHealthcare. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From idimitro <@t> mun.ca Thu May 24 07:37:48 2012 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Thu May 24 07:38:00 2012 Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Message-ID: <14C3108E8B98EF43B3EDAE5833670034152E7A@exchange.med.mun.ca> Dear HistoNet Community, Recently we purchased an immunostainer . In the waste from the immunostainer there are small quantities of 3,3'-Diaminobenzidine Tetrahydrochloride (DAB) in the amount of e 35-38 mg per 20L of waste. I want to hear from the community how you dispose of it. Do you use any of the methods available (permanganate/sulphuric acid and hydrogen peroxide/HRPA) to convert DAB to non-mutagenic compounds? Thank you, Iliana Dimitrova, RT, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php From Joyce.Weems <@t> emoryhealthcare.org Thu May 24 08:58:12 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu May 24 08:58:22 2012 Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) In-Reply-To: <14C3108E8B98EF43B3EDAE5833670034152E7A@exchange.med.mun.ca> References: <14C3108E8B98EF43B3EDAE5833670034152E7A@exchange.med.mun.ca> Message-ID: We have ours hauled away by our hazardous waste company.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of idimitro@mun.ca Sent: Thursday, May 24, 2012 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Dear HistoNet Community, Recently we purchased an immunostainer . In the waste from the immunostainer there are small quantities of 3,3'-Diaminobenzidine Tetrahydrochloride (DAB) in the amount of e 35-38 mg per 20L of waste. I want to hear from the community how you dispose of it. Do you use any of the methods available (permanganate/sulphuric acid and hydrogen peroxide/HRPA) to convert DAB to non-mutagenic compounds? Thank you, Iliana Dimitrova, RT, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From relia1 <@t> earthlink.net Thu May 24 09:17:35 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu May 24 09:18:00 2012 Subject: [Histonet] Exciting new opportunities in Philadelphia, PA and Phoenix, AZ These are RELIA EXCLUSIVES!! Message-ID: <000001cd39b7$f7f57730$e7e06590$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day. I have new opportunities that are exclusive to RELIA that I want to tell you about. I am excited about these positions because they are new clients for me who I have heard really great things about from your peers. I have a new client in the Phoenix area that is looking for a histotech ht/htl certified with at least 2 years of experience. Derm experience is preferred and Mohs is a plus - if you don't know Mohs they will train. I have a new client who we are helping with staffing in their brand new lab located just north of Philadelphia. ASCP HT/HTL and AAS or BS degree required along with a minimum of 3 years of experience with routine histology. Grossing and/or IHC experience are a plus. My clients offer competitive salaries and excellent benefits. If you or anyone you know might be interested in hearing more about either of these opportunities please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From joelleweaver <@t> hotmail.com Thu May 24 09:25:44 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 24 09:25:54 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <29496B3FDE874BA18B4B3A7A07111F37@HP2010> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov>, , <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> , <29496B3FDE874BA18B4B3A7A07111F37@HP2010> Message-ID: Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lpwenk@sbcglobal.net > To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org > CC: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > Date: Thu, 24 May 2012 05:43:32 -0400 > > I'd like to wade into this discuss with a couple of comments: > > LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": > Yes, I'm still hearing about places like this. When I talk with the > supervisors, it's because the lab wants the person doing the "histotech" > job, but they only want to pay them at "lab assistant" wages. Plus, once > they get the people trained as "histotechs", the employees can't go > elsewhere, because the other labs only want certified histotech, and these > people can't get certified as they don't have the associate degree and > minimum 12 hours of biology and chemistry combined as required to take the > ASCP HT exam. So these people end up having to stay there. (Personally, I > think is very unfair to the employees they hire.) > > LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: > Even though the High School route was dropped as of Jan 1, 2005 (over 7 > years ago), I still get emails from labs that want to hire one of my > students, but their job description says high school diploma. I usually call > these places up, and the histology supervisor had no idea the ASCP HT high > school route was dropped. "Someone" should have told them. Even though it > was in every "NSH in Action" for the 5 years previous (that's now over 12 > years ago), in some ASCP publications each year for the 5 years previous, > and on both the NSH and ASCP webpage for the 5 years previous, well, since > they aren't NSH or ASCP members, well, "someone" still should have contacted > them directly and let them know. Sigh. > > I've had employees call that they were hired after the 2005 deadline, with > the job description of high school graduate requirement, and were told they > had 2 years to get the experience required, and then they had 1 additional > year in which to take and pass the HT exam. And when they went to sign up to > take the HT exam, they discovered that the HT exam requirements had dropped > the high school route and now the on-the-job (OJT) requires the associate > degree/60 credit hours with 12 credits of bio/chem, which of course they > don't have. They tell me that their histology supervisor says they are going > to fire them, because they can't take the ASCP HT Exam. I end up talking > with the supervisor, and advise them to talk with their HR and Legal > departments, as they are the ones who advertised the high school > requirement, and they are the ones who hired this person without the needed > education. And I suggest they help with person complete an on-line NAACLS HT > program, several of which will take someone with the high school diploma, as > long as they had a biology, a chemistry, and a math class in high school. > > NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: > NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, but > for most lab training programs.) NAACLS has a long list of standards for > programs to follow. (Think CAP checklist.) > > Standard 14 G has a statement "The granting of the degree or certificate > must not be contingent upon the student's passing any type of external > certification or licensure examination." > > (Explanation: Not all HT programs end in an associate degree. The > certificate refers to a certificate of completion of a program. My program, > for example, is hospital-based. Some students already have their degree > before they start my program. Some have all the college credits except for > the ones they are earning while completing the internship, then they earn > their degree from the college when they complete the internship and get the > grade for those last credit hours. The hospital doesn't grant the degree, > the college does. The hospital program grants a certificate of completion of > the program, which is acceptable to NAACLS, ASCP, and employers.) > > As NAACLS accredited HT or HTL programs, we can encourage our students to > take the HT/HTL exam upon completion of the program. We can do review > sessions with them. We can remind them of the deadlines to sign up. We can > help them sign up if they are having problems. We can let them know that > labs in our area expect people to be certified. We can let them know that > they can sign up while still in the program (couple of months before > graduation), and they can, before they graduate, pick a date to take the > exam after graduation. We can tell them that these dates to take the exams > can be put on their resume, on the application, and that they can inform the > supervisor during the interview that they are already signed up to take the > HT/HTL exam. > > But we can NOT "make" the student take the exam. Completion of the program > cannot be contingent upon taking or passing the HT/HTL exam (or getting > state licensure). The program could lose NAACLS accreditation if we force > the student to take the HT/HTL/state licensure exam, or withhold their > degree or certificate until they do take/pass the HT/HTL exam/become state > licensed. > > Thanks for "listening". > > Peggy A. Wenk, HTL(ASCP)SLS > Program Director, Schools of Histotechnology > William Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own, and do not represent Beaumont Hospital.) > > -----Original Message----- > From: joelle weaver > Sent: Wednesday, May 23, 2012 12:41 PM > To: tpodawiltz@lrgh.org > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) > > > TomI compliment your approach, and I personally really like to see when > employers do this. I think it provides opportunity, yet incentive. Two years > seems especially reasonable. Most places that I have known who use this > method for new-grad hires, have allowed 1 year. It is even better when they > provide additional training and support to the person as they are preparing. > Having been involved in an online program and clinical curricula in the > past, I feel from my observations that continuity with work in the > environment is essential for most in connecting the theory with execution. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > From: tpodawiltz@lrgh.org > > To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 23 May 2012 10:45:52 -0400 > > Subject: RE: [Histonet] (no subject) > > > > The last person I hired for a tech position, was just finishing her > > on-line program. I helped her complete that and then she was given two > > years to get her certification. This was all part of her hiring agreement, > > so she knew this coming in and completely understood that failing to > > achieve either one would affect her employment. > > > > > > > > Tom Podawiltz HT (ASCP) > > Histology Section Head/Laboratory Safety Officer. > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > > weaver > > Sent: Tuesday, May 22, 2012 1:43 PM > > To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] (no subject) > > > > > > That seems to be the unfortunate situation at this time... > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Tue, 22 May 2012 12:39:39 -0500 > > > From: Sherrian.McAnn@va.gov > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] (no subject) > > > > > > I agree and would like to add. This is one scenario that I have seen > > > many times, where hospitals or wherever will hire histotechs without > > > certifications . I am thinking that saves them money and they still > > > have a "histotech". I have seen good histotechs that have no > > > certification and likewise some bad ones with certification. Lately I > > > have seen these schools turn out histotechs , it seems with little > > > encouragement to get certified. If places will hire them without being > > > certified, there seems little incentive (unless you are self motivated > > > for more money) to move on up to certification. > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > > This e-mail message and any attachments are proprietary and confidential > > information intended only for the use of the recipient(s) named above. If > > you are not the intended recipient, you may not print,distribute, or copy > > this message or any attachments. If you have received this communication > > in error, please notify the sender by return e-mail and delete this > > message and any attachments from your computer. Any views or opinions > > expressed are solely those of the author and do not necessarily represent > > those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b-frederick <@t> northwestern.edu Thu May 24 09:45:44 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu May 24 09:45:54 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov>, , <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> , <29496B3FDE874BA18B4B3A7A07111F37@HP2010> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 24, 2012 9:26 AM To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lpwenk@sbcglobal.net > To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org > CC: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > Date: Thu, 24 May 2012 05:43:32 -0400 > > I'd like to wade into this discuss with a couple of comments: > > LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": > Yes, I'm still hearing about places like this. When I talk with the > supervisors, it's because the lab wants the person doing the "histotech" > job, but they only want to pay them at "lab assistant" wages. Plus, > once they get the people trained as "histotechs", the employees can't > go elsewhere, because the other labs only want certified histotech, > and these people can't get certified as they don't have the associate > degree and minimum 12 hours of biology and chemistry combined as > required to take the ASCP HT exam. So these people end up having to > stay there. (Personally, I think is very unfair to the employees they > hire.) > > LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: > Even though the High School route was dropped as of Jan 1, 2005 (over > 7 years ago), I still get emails from labs that want to hire one of > my students, but their job description says high school diploma. I > usually call these places up, and the histology supervisor had no idea > the ASCP HT high school route was dropped. "Someone" should have told > them. Even though it was in every "NSH in Action" for the 5 years > previous (that's now over 12 years ago), in some ASCP publications > each year for the 5 years previous, and on both the NSH and ASCP > webpage for the 5 years previous, well, since they aren't NSH or ASCP > members, well, "someone" still should have contacted them directly and let them know. Sigh. > > I've had employees call that they were hired after the 2005 deadline, > with the job description of high school graduate requirement, and were > told they had 2 years to get the experience required, and then they > had 1 additional year in which to take and pass the HT exam. And when > they went to sign up to take the HT exam, they discovered that the HT > exam requirements had dropped the high school route and now the > on-the-job (OJT) requires the associate > degree/60 credit hours with 12 credits of bio/chem, which of course > they don't have. They tell me that their histology supervisor says > they are going to fire them, because they can't take the ASCP HT Exam. > I end up talking with the supervisor, and advise them to talk with > their HR and Legal departments, as they are the ones who advertised > the high school requirement, and they are the ones who hired this > person without the needed education. And I suggest they help with > person complete an on-line NAACLS HT program, several of which will > take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. > > NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: > NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, > but for most lab training programs.) NAACLS has a long list of > standards for programs to follow. (Think CAP checklist.) > > Standard 14 G has a statement "The granting of the degree or > certificate must not be contingent upon the student's passing any type > of external certification or licensure examination." > > (Explanation: Not all HT programs end in an associate degree. The > certificate refers to a certificate of completion of a program. My > program, for example, is hospital-based. Some students already have > their degree before they start my program. Some have all the college > credits except for the ones they are earning while completing the > internship, then they earn their degree from the college when they > complete the internship and get the grade for those last credit hours. > The hospital doesn't grant the degree, the college does. The hospital > program grants a certificate of completion of the program, which is > acceptable to NAACLS, ASCP, and employers.) > > As NAACLS accredited HT or HTL programs, we can encourage our students > to take the HT/HTL exam upon completion of the program. We can do > review sessions with them. We can remind them of the deadlines to sign > up. We can help them sign up if they are having problems. We can let > them know that labs in our area expect people to be certified. We can > let them know that they can sign up while still in the program (couple > of months before graduation), and they can, before they graduate, pick > a date to take the exam after graduation. We can tell them that these > dates to take the exams can be put on their resume, on the > application, and that they can inform the supervisor during the > interview that they are already signed up to take the HT/HTL exam. > > But we can NOT "make" the student take the exam. Completion of the > program cannot be contingent upon taking or passing the HT/HTL exam > (or getting state licensure). The program could lose NAACLS > accreditation if we force the student to take the HT/HTL/state > licensure exam, or withhold their degree or certificate until they do > take/pass the HT/HTL exam/become state licensed. > > Thanks for "listening". > > Peggy A. Wenk, HTL(ASCP)SLS > Program Director, Schools of Histotechnology William Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own, and do not represent Beaumont > Hospital.) > > -----Original Message----- > From: joelle weaver > Sent: Wednesday, May 23, 2012 12:41 PM > To: tpodawiltz@lrgh.org > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) > > > TomI compliment your approach, and I personally really like to see > when employers do this. I think it provides opportunity, yet > incentive. Two years seems especially reasonable. Most places that I > have known who use this method for new-grad hires, have allowed 1 > year. It is even better when they provide additional training and support to the person as they are preparing. > Having been involved in an online program and clinical curricula in > the past, I feel from my observations that continuity with work in the > environment is essential for most in connecting the theory with execution. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > From: tpodawiltz@lrgh.org > > To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 23 May 2012 10:45:52 -0400 > > Subject: RE: [Histonet] (no subject) > > > > The last person I hired for a tech position, was just finishing her > > on-line program. I helped her complete that and then she was given > > two years to get her certification. This was all part of her hiring > > agreement, so she knew this coming in and completely understood that > > failing to achieve either one would affect her employment. > > > > > > > > Tom Podawiltz HT (ASCP) > > Histology Section Head/Laboratory Safety Officer. > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > joelle weaver > > Sent: Tuesday, May 22, 2012 1:43 PM > > To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] (no subject) > > > > > > That seems to be the unfortunate situation at this time... > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Tue, 22 May 2012 12:39:39 -0500 > > > From: Sherrian.McAnn@va.gov > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] (no subject) > > > > > > I agree and would like to add. This is one scenario that I have > > > seen many times, where hospitals or wherever will hire histotechs > > > without certifications . I am thinking that saves them money and > > > they still have a "histotech". I have seen good histotechs that > > > have no certification and likewise some bad ones with certification. Lately I > > > have seen these schools turn out histotechs , it seems with little > > > encouragement to get certified. If places will hire them without > > > being certified, there seems little incentive (unless you are > > > self motivated for more money) to move on up to certification. > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > > This e-mail message and any attachments are proprietary and > > confidential information intended only for the use of the > > recipient(s) named above. If you are not the intended recipient, you > > may not print,distribute, or copy this message or any attachments. > > If you have received this communication in error, please notify the > > sender by return e-mail and delete this message and any attachments > > from your computer. Any views or opinions expressed are solely those > > of the author and do not necessarily represent those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Thu May 24 09:50:23 2012 From: chapcl <@t> yahoo.com (William) Date: Thu May 24 09:50:45 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> <29496B3FDE874BA18B4B3A7A07111F37@HP2010> <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> Message-ID: <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> OJT is only available to HTL's via the route you described. Sent from my iPhone On May 24, 2012, at 10:45 AM, Bernice Frederick wrote: > If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, May 24, 2012 9:26 AM > To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > > > Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: lpwenk@sbcglobal.net >> To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org >> CC: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) >> Date: Thu, 24 May 2012 05:43:32 -0400 >> >> I'd like to wade into this discuss with a couple of comments: >> >> LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": >> Yes, I'm still hearing about places like this. When I talk with the >> supervisors, it's because the lab wants the person doing the "histotech" >> job, but they only want to pay them at "lab assistant" wages. Plus, >> once they get the people trained as "histotechs", the employees can't >> go elsewhere, because the other labs only want certified histotech, >> and these people can't get certified as they don't have the associate >> degree and minimum 12 hours of biology and chemistry combined as >> required to take the ASCP HT exam. So these people end up having to >> stay there. (Personally, I think is very unfair to the employees they >> hire.) >> >> LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: >> Even though the High School route was dropped as of Jan 1, 2005 (over >> 7 years ago), I still get emails from labs that want to hire one of >> my students, but their job description says high school diploma. I >> usually call these places up, and the histology supervisor had no idea >> the ASCP HT high school route was dropped. "Someone" should have told >> them. Even though it was in every "NSH in Action" for the 5 years >> previous (that's now over 12 years ago), in some ASCP publications >> each year for the 5 years previous, and on both the NSH and ASCP >> webpage for the 5 years previous, well, since they aren't NSH or ASCP >> members, well, "someone" still should have contacted them directly and let them know. Sigh. >> >> I've had employees call that they were hired after the 2005 deadline, >> with the job description of high school graduate requirement, and were >> told they had 2 years to get the experience required, and then they >> had 1 additional year in which to take and pass the HT exam. And when >> they went to sign up to take the HT exam, they discovered that the HT >> exam requirements had dropped the high school route and now the >> on-the-job (OJT) requires the associate >> degree/60 credit hours with 12 credits of bio/chem, which of course >> they don't have. They tell me that their histology supervisor says >> they are going to fire them, because they can't take the ASCP HT Exam. >> I end up talking with the supervisor, and advise them to talk with >> their HR and Legal departments, as they are the ones who advertised >> the high school requirement, and they are the ones who hired this >> person without the needed education. And I suggest they help with >> person complete an on-line NAACLS HT program, several of which will >> take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. >> >> NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: >> NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, >> but for most lab training programs.) NAACLS has a long list of >> standards for programs to follow. (Think CAP checklist.) >> >> Standard 14 G has a statement "The granting of the degree or >> certificate must not be contingent upon the student's passing any type >> of external certification or licensure examination." >> >> (Explanation: Not all HT programs end in an associate degree. The >> certificate refers to a certificate of completion of a program. My >> program, for example, is hospital-based. Some students already have >> their degree before they start my program. Some have all the college >> credits except for the ones they are earning while completing the >> internship, then they earn their degree from the college when they >> complete the internship and get the grade for those last credit hours. >> The hospital doesn't grant the degree, the college does. The hospital >> program grants a certificate of completion of the program, which is >> acceptable to NAACLS, ASCP, and employers.) >> >> As NAACLS accredited HT or HTL programs, we can encourage our students >> to take the HT/HTL exam upon completion of the program. We can do >> review sessions with them. We can remind them of the deadlines to sign >> up. We can help them sign up if they are having problems. We can let >> them know that labs in our area expect people to be certified. We can >> let them know that they can sign up while still in the program (couple >> of months before graduation), and they can, before they graduate, pick >> a date to take the exam after graduation. We can tell them that these >> dates to take the exams can be put on their resume, on the >> application, and that they can inform the supervisor during the >> interview that they are already signed up to take the HT/HTL exam. >> >> But we can NOT "make" the student take the exam. Completion of the >> program cannot be contingent upon taking or passing the HT/HTL exam >> (or getting state licensure). The program could lose NAACLS >> accreditation if we force the student to take the HT/HTL/state >> licensure exam, or withhold their degree or certificate until they do >> take/pass the HT/HTL exam/become state licensed. >> >> Thanks for "listening". >> >> Peggy A. Wenk, HTL(ASCP)SLS >> Program Director, Schools of Histotechnology William Beaumont Hospital >> Royal Oak, MI 48073 >> >> (The opinions expressed are my own, and do not represent Beaumont >> Hospital.) >> >> -----Original Message----- >> From: joelle weaver >> Sent: Wednesday, May 23, 2012 12:41 PM >> To: tpodawiltz@lrgh.org >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] (no subject) >> >> >> TomI compliment your approach, and I personally really like to see >> when employers do this. I think it provides opportunity, yet >> incentive. Two years seems especially reasonable. Most places that I >> have known who use this method for new-grad hires, have allowed 1 >> year. It is even better when they provide additional training and support to the person as they are preparing. >> Having been involved in an online program and clinical curricula in >> the past, I feel from my observations that continuity with work in the >> environment is essential for most in connecting the theory with execution. >> >> >> >> Joelle Weaver MAOM, HTL (ASCP) QIHC >>> From: tpodawiltz@lrgh.org >>> To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; >>> histonet@lists.utsouthwestern.edu >>> Date: Wed, 23 May 2012 10:45:52 -0400 >>> Subject: RE: [Histonet] (no subject) >>> >>> The last person I hired for a tech position, was just finishing her >>> on-line program. I helped her complete that and then she was given >>> two years to get her certification. This was all part of her hiring >>> agreement, so she knew this coming in and completely understood that >>> failing to achieve either one would affect her employment. >>> >>> >>> >>> Tom Podawiltz HT (ASCP) >>> Histology Section Head/Laboratory Safety Officer. >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>> joelle weaver >>> Sent: Tuesday, May 22, 2012 1:43 PM >>> To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] (no subject) >>> >>> >>> That seems to be the unfortunate situation at this time... >>> >>> >>> >>> >>> Joelle Weaver MAOM, HTL (ASCP) QIHC >>>> Date: Tue, 22 May 2012 12:39:39 -0500 >>>> From: Sherrian.McAnn@va.gov >>>> To: histonet@lists.utsouthwestern.edu >>>> Subject: [Histonet] (no subject) >>>> >>>> I agree and would like to add. This is one scenario that I have >>>> seen many times, where hospitals or wherever will hire histotechs >>>> without certifications . I am thinking that saves them money and >>>> they still have a "histotech". I have seen good histotechs that >>>> have no certification and likewise some bad ones with certification. Lately I >>>> have seen these schools turn out histotechs , it seems with little >>>> encouragement to get certified. If places will hire them without >>>> being certified, there seems little incentive (unless you are >>>> self motivated for more money) to move on up to certification. >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> THIS MESSAGE IS CONFIDENTIAL. >>> This e-mail message and any attachments are proprietary and >>> confidential information intended only for the use of the >>> recipient(s) named above. If you are not the intended recipient, you >>> may not print,distribute, or copy this message or any attachments. >>> If you have received this communication in error, please notify the >>> sender by return e-mail and delete this message and any attachments >>> from your computer. Any views or opinions expressed are solely those >>> of the author and do not necessarily represent those of LRGHealthcare. >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu May 24 09:52:16 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu May 24 09:52:27 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> <29496B3FDE874BA18B4B3A7A07111F37@HP2010> <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E252167@evcspmbx3.ads.northwestern.edu> So why was OJT supposedly off the charts in 2005 (so to speak). Guess not. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: William [mailto:chapcl@yahoo.com] Sent: Thursday, May 24, 2012 9:50 AM To: Bernice Frederick Cc: joelle weaver; lpwenk@sbcglobal.net; tpodawiltz@lrgh.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) OJT is only available to HTL's via the route you described. Sent from my iPhone On May 24, 2012, at 10:45 AM, Bernice Frederick wrote: > If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Thursday, May 24, 2012 9:26 AM > To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > > > Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: lpwenk@sbcglobal.net >> To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org >> CC: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) >> Date: Thu, 24 May 2012 05:43:32 -0400 >> >> I'd like to wade into this discuss with a couple of comments: >> >> LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": >> Yes, I'm still hearing about places like this. When I talk with the >> supervisors, it's because the lab wants the person doing the "histotech" >> job, but they only want to pay them at "lab assistant" wages. Plus, >> once they get the people trained as "histotechs", the employees can't >> go elsewhere, because the other labs only want certified histotech, >> and these people can't get certified as they don't have the associate >> degree and minimum 12 hours of biology and chemistry combined as >> required to take the ASCP HT exam. So these people end up having to >> stay there. (Personally, I think is very unfair to the employees they >> hire.) >> >> LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: >> Even though the High School route was dropped as of Jan 1, 2005 (over >> 7 years ago), I still get emails from labs that want to hire one of >> my students, but their job description says high school diploma. I >> usually call these places up, and the histology supervisor had no >> idea the ASCP HT high school route was dropped. "Someone" should have >> told them. Even though it was in every "NSH in Action" for the 5 >> years previous (that's now over 12 years ago), in some ASCP >> publications each year for the 5 years previous, and on both the NSH >> and ASCP webpage for the 5 years previous, well, since they aren't >> NSH or ASCP members, well, "someone" still should have contacted them directly and let them know. Sigh. >> >> I've had employees call that they were hired after the 2005 deadline, >> with the job description of high school graduate requirement, and >> were told they had 2 years to get the experience required, and then >> they had 1 additional year in which to take and pass the HT exam. And >> when they went to sign up to take the HT exam, they discovered that >> the HT exam requirements had dropped the high school route and now >> the on-the-job (OJT) requires the associate >> degree/60 credit hours with 12 credits of bio/chem, which of course >> they don't have. They tell me that their histology supervisor says >> they are going to fire them, because they can't take the ASCP HT Exam. >> I end up talking with the supervisor, and advise them to talk with >> their HR and Legal departments, as they are the ones who advertised >> the high school requirement, and they are the ones who hired this >> person without the needed education. And I suggest they help with >> person complete an on-line NAACLS HT program, several of which will >> take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. >> >> NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: >> NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, >> but for most lab training programs.) NAACLS has a long list of >> standards for programs to follow. (Think CAP checklist.) >> >> Standard 14 G has a statement "The granting of the degree or >> certificate must not be contingent upon the student's passing any >> type of external certification or licensure examination." >> >> (Explanation: Not all HT programs end in an associate degree. The >> certificate refers to a certificate of completion of a program. My >> program, for example, is hospital-based. Some students already have >> their degree before they start my program. Some have all the college >> credits except for the ones they are earning while completing the >> internship, then they earn their degree from the college when they >> complete the internship and get the grade for those last credit hours. >> The hospital doesn't grant the degree, the college does. The hospital >> program grants a certificate of completion of the program, which is >> acceptable to NAACLS, ASCP, and employers.) >> >> As NAACLS accredited HT or HTL programs, we can encourage our >> students to take the HT/HTL exam upon completion of the program. We >> can do review sessions with them. We can remind them of the deadlines >> to sign up. We can help them sign up if they are having problems. We >> can let them know that labs in our area expect people to be >> certified. We can let them know that they can sign up while still in >> the program (couple of months before graduation), and they can, >> before they graduate, pick a date to take the exam after graduation. >> We can tell them that these dates to take the exams can be put on >> their resume, on the application, and that they can inform the >> supervisor during the interview that they are already signed up to take the HT/HTL exam. >> >> But we can NOT "make" the student take the exam. Completion of the >> program cannot be contingent upon taking or passing the HT/HTL exam >> (or getting state licensure). The program could lose NAACLS >> accreditation if we force the student to take the HT/HTL/state >> licensure exam, or withhold their degree or certificate until they do >> take/pass the HT/HTL exam/become state licensed. >> >> Thanks for "listening". >> >> Peggy A. Wenk, HTL(ASCP)SLS >> Program Director, Schools of Histotechnology William Beaumont >> Hospital Royal Oak, MI 48073 >> >> (The opinions expressed are my own, and do not represent Beaumont >> Hospital.) >> >> -----Original Message----- >> From: joelle weaver >> Sent: Wednesday, May 23, 2012 12:41 PM >> To: tpodawiltz@lrgh.org >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] (no subject) >> >> >> TomI compliment your approach, and I personally really like to see >> when employers do this. I think it provides opportunity, yet >> incentive. Two years seems especially reasonable. Most places that I >> have known who use this method for new-grad hires, have allowed 1 >> year. It is even better when they provide additional training and support to the person as they are preparing. >> Having been involved in an online program and clinical curricula in >> the past, I feel from my observations that continuity with work in >> the environment is essential for most in connecting the theory with execution. >> >> >> >> Joelle Weaver MAOM, HTL (ASCP) QIHC >>> From: tpodawiltz@lrgh.org >>> To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; >>> histonet@lists.utsouthwestern.edu >>> Date: Wed, 23 May 2012 10:45:52 -0400 >>> Subject: RE: [Histonet] (no subject) >>> >>> The last person I hired for a tech position, was just finishing her >>> on-line program. I helped her complete that and then she was given >>> two years to get her certification. This was all part of her hiring >>> agreement, so she knew this coming in and completely understood that >>> failing to achieve either one would affect her employment. >>> >>> >>> >>> Tom Podawiltz HT (ASCP) >>> Histology Section Head/Laboratory Safety Officer. >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>> joelle weaver >>> Sent: Tuesday, May 22, 2012 1:43 PM >>> To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] (no subject) >>> >>> >>> That seems to be the unfortunate situation at this time... >>> >>> >>> >>> >>> Joelle Weaver MAOM, HTL (ASCP) QIHC >>>> Date: Tue, 22 May 2012 12:39:39 -0500 >>>> From: Sherrian.McAnn@va.gov >>>> To: histonet@lists.utsouthwestern.edu >>>> Subject: [Histonet] (no subject) >>>> >>>> I agree and would like to add. This is one scenario that I have >>>> seen many times, where hospitals or wherever will hire histotechs >>>> without certifications . I am thinking that saves them money and >>>> they still have a "histotech". I have seen good histotechs that >>>> have no certification and likewise some bad ones with certification. Lately I >>>> have seen these schools turn out histotechs , it seems with little >>>> encouragement to get certified. If places will hire them without >>>> being certified, there seems little incentive (unless you are self >>>> motivated for more money) to move on up to certification. >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> THIS MESSAGE IS CONFIDENTIAL. >>> This e-mail message and any attachments are proprietary and >>> confidential information intended only for the use of the >>> recipient(s) named above. If you are not the intended recipient, you >>> may not print,distribute, or copy this message or any attachments. >>> If you have received this communication in error, please notify the >>> sender by return e-mail and delete this message and any attachments >>> from your computer. Any views or opinions expressed are solely those >>> of the author and do not necessarily represent those of LRGHealthcare. >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 24 09:57:17 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 24 09:57:25 2012 Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) In-Reply-To: <14C3108E8B98EF43B3EDAE5833670034152E7A@exchange.med.mun.ca> Message-ID: <1337871437.63943.YahooMailClassic@web162106.mail.bf1.yahoo.com> I used to neutralize DAB with 0.2M potassium permanganate + 2.0 M sulfuric + decolorize with ascorbic acid, but the procedure is too cumbersome. Others prefer to give the waste to a company that will dispose of it BUT remember that no matter who "disposes" of your wastes, YOU and NOT the disposing company are responsible is something happens, The same happens with disposing of used formalin and xylene. Ren? J. --- On Thu, 5/24/12, idimitro@mun.ca wrote: From: idimitro@mun.ca Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) To: histonet@lists.utsouthwestern.edu Date: Thursday, May 24, 2012, 8:37 AM Dear HistoNet Community, Recently we purchased an immunostainer . In the waste from the immunostainer there are small quantities of 3,3'-Diaminobenzidine Tetrahydrochloride (DAB) in the amount of e 35-38 mg per 20L of waste. I want to hear from the community how you dispose of it. Do you use any of the methods available (permanganate/sulphuric acid and hydrogen peroxide/HRPA) to convert DAB to non-mutagenic compounds? Thank you, Iliana Dimitrova, RT, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu May 24 10:09:38 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu May 24 10:11:55 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov>, , <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> , <29496B3FDE874BA18B4B3A7A07111F37@HP2010> , <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC7FAF@SBS2K8.premierlab.local> Bernice I hire individuals with BS and train them in the lab and then they sit for the HT or HTL registry after one year of employment. I have done this consistently over the years. I have had probably about 8 or so individuals train with me and then pass the registry. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick [b-frederick@northwestern.edu] Sent: Thursday, May 24, 2012 8:45 AM To: joelle weaver; lpwenk@sbcglobal.net; tpodawiltz@lrgh.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 24, 2012 9:26 AM To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lpwenk@sbcglobal.net > To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org > CC: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > Date: Thu, 24 May 2012 05:43:32 -0400 > > I'd like to wade into this discuss with a couple of comments: > > LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": > Yes, I'm still hearing about places like this. When I talk with the > supervisors, it's because the lab wants the person doing the "histotech" > job, but they only want to pay them at "lab assistant" wages. Plus, > once they get the people trained as "histotechs", the employees can't > go elsewhere, because the other labs only want certified histotech, > and these people can't get certified as they don't have the associate > degree and minimum 12 hours of biology and chemistry combined as > required to take the ASCP HT exam. So these people end up having to > stay there. (Personally, I think is very unfair to the employees they > hire.) > > LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: > Even though the High School route was dropped as of Jan 1, 2005 (over > 7 years ago), I still get emails from labs that want to hire one of > my students, but their job description says high school diploma. I > usually call these places up, and the histology supervisor had no idea > the ASCP HT high school route was dropped. "Someone" should have told > them. Even though it was in every "NSH in Action" for the 5 years > previous (that's now over 12 years ago), in some ASCP publications > each year for the 5 years previous, and on both the NSH and ASCP > webpage for the 5 years previous, well, since they aren't NSH or ASCP > members, well, "someone" still should have contacted them directly and let them know. Sigh. > > I've had employees call that they were hired after the 2005 deadline, > with the job description of high school graduate requirement, and were > told they had 2 years to get the experience required, and then they > had 1 additional year in which to take and pass the HT exam. And when > they went to sign up to take the HT exam, they discovered that the HT > exam requirements had dropped the high school route and now the > on-the-job (OJT) requires the associate > degree/60 credit hours with 12 credits of bio/chem, which of course > they don't have. They tell me that their histology supervisor says > they are going to fire them, because they can't take the ASCP HT Exam. > I end up talking with the supervisor, and advise them to talk with > their HR and Legal departments, as they are the ones who advertised > the high school requirement, and they are the ones who hired this > person without the needed education. And I suggest they help with > person complete an on-line NAACLS HT program, several of which will > take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. > > NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: > NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, > but for most lab training programs.) NAACLS has a long list of > standards for programs to follow. (Think CAP checklist.) > > Standard 14 G has a statement "The granting of the degree or > certificate must not be contingent upon the student's passing any type > of external certification or licensure examination." > > (Explanation: Not all HT programs end in an associate degree. The > certificate refers to a certificate of completion of a program. My > program, for example, is hospital-based. Some students already have > their degree before they start my program. Some have all the college > credits except for the ones they are earning while completing the > internship, then they earn their degree from the college when they > complete the internship and get the grade for those last credit hours. > The hospital doesn't grant the degree, the college does. The hospital > program grants a certificate of completion of the program, which is > acceptable to NAACLS, ASCP, and employers.) > > As NAACLS accredited HT or HTL programs, we can encourage our students > to take the HT/HTL exam upon completion of the program. We can do > review sessions with them. We can remind them of the deadlines to sign > up. We can help them sign up if they are having problems. We can let > them know that labs in our area expect people to be certified. We can > let them know that they can sign up while still in the program (couple > of months before graduation), and they can, before they graduate, pick > a date to take the exam after graduation. We can tell them that these > dates to take the exams can be put on their resume, on the > application, and that they can inform the supervisor during the > interview that they are already signed up to take the HT/HTL exam. > > But we can NOT "make" the student take the exam. Completion of the > program cannot be contingent upon taking or passing the HT/HTL exam > (or getting state licensure). The program could lose NAACLS > accreditation if we force the student to take the HT/HTL/state > licensure exam, or withhold their degree or certificate until they do > take/pass the HT/HTL exam/become state licensed. > > Thanks for "listening". > > Peggy A. Wenk, HTL(ASCP)SLS > Program Director, Schools of Histotechnology William Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own, and do not represent Beaumont > Hospital.) > > -----Original Message----- > From: joelle weaver > Sent: Wednesday, May 23, 2012 12:41 PM > To: tpodawiltz@lrgh.org > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) > > > TomI compliment your approach, and I personally really like to see > when employers do this. I think it provides opportunity, yet > incentive. Two years seems especially reasonable. Most places that I > have known who use this method for new-grad hires, have allowed 1 > year. It is even better when they provide additional training and support to the person as they are preparing. > Having been involved in an online program and clinical curricula in > the past, I feel from my observations that continuity with work in the > environment is essential for most in connecting the theory with execution. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > From: tpodawiltz@lrgh.org > > To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 23 May 2012 10:45:52 -0400 > > Subject: RE: [Histonet] (no subject) > > > > The last person I hired for a tech position, was just finishing her > > on-line program. I helped her complete that and then she was given > > two years to get her certification. This was all part of her hiring > > agreement, so she knew this coming in and completely understood that > > failing to achieve either one would affect her employment. > > > > > > > > Tom Podawiltz HT (ASCP) > > Histology Section Head/Laboratory Safety Officer. > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > joelle weaver > > Sent: Tuesday, May 22, 2012 1:43 PM > > To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] (no subject) > > > > > > That seems to be the unfortunate situation at this time... > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Tue, 22 May 2012 12:39:39 -0500 > > > From: Sherrian.McAnn@va.gov > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] (no subject) > > > > > > I agree and would like to add. This is one scenario that I have > > > seen many times, where hospitals or wherever will hire histotechs > > > without certifications . I am thinking that saves them money and > > > they still have a "histotech". I have seen good histotechs that > > > have no certification and likewise some bad ones with certification. Lately I > > > have seen these schools turn out histotechs , it seems with little > > > encouragement to get certified. If places will hire them without > > > being certified, there seems little incentive (unless you are > > > self motivated for more money) to move on up to certification. > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > > This e-mail message and any attachments are proprietary and > > confidential information intended only for the use of the > > recipient(s) named above. If you are not the intended recipient, you > > may not print,distribute, or copy this message or any attachments. > > If you have received this communication in error, please notify the > > sender by return e-mail and delete this message and any attachments > > from your computer. Any views or opinions expressed are solely those > > of the author and do not necessarily represent those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu May 24 10:37:19 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu May 24 10:38:03 2012 Subject: [Histonet] Elastic Stain Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF573@mmc-mail.ad.mhsil.com> We are still looking for an Elastic/H&E stain procedure for lung tissue. Please submit procedures to Jim Vickroy. vickroy.jim@mhsil.com A few of you have responded with some suggestions but we still have not found the right procedure. Currently we stain for elastic with Verhoeff's Elastic Stain and use Van Gieson's stain as a counterstain and the pathologist would like us to use a hematoxylin and eosin counterstain instead. We have tried to use the H&E as a counterstain but it is still not where he wants it to be. Does anybody have some experience with this that they would like to share? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From renafail2 <@t> gmail.com Thu May 24 10:53:25 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Thu May 24 10:53:38 2012 Subject: [Histonet] Elastic Stain In-Reply-To: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF573@mmc-mail.ad.mhsil.com> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF573@mmc-mail.ad.mhsil.com> Message-ID: Have you tried Resorcin fucshin H&E? 1 g RF 100 ml 70% ethanol 1 ml HCL Stain in RF solution 15-30 minutes , according to depth desired wash off excess stain in 95% alcohol Wash in tap water 3-5 minutes Follow with routine H&E Rena Fail On Thu, May 24, 2012 at 11:37 AM, Vickroy, Jim wrote: > We are still looking for an Elastic/H&E stain procedure for lung tissue. > Please submit procedures to Jim Vickroy. vickroy.jim@mhsil.com vickroy.jim@mhsil.com> > > A few of you have responded with some suggestions but we still have not > found the right procedure. Currently we stain for elastic with Verhoeff's > Elastic Stain and use Van Gieson's stain as a counterstain and the > pathologist would like us to use a hematoxylin and eosin counterstain > instead. We have tried to use the H&E as a counterstain but it is still > not where he wants it to be. Does anybody have some experience with this > that they would like to share? > > Thanks > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Thu May 24 11:23:45 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 24 11:23:55 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E252167@evcspmbx3.ads.northwestern.edu> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> <29496B3FDE874BA18B4B3A7A07111F37@HP2010> <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu> <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> <62C639732D3F274DACED033EBDF6ADAF1E252167@evcspmbx3.ads.northwestern.edu> Message-ID: <8D7C2D242DBD45498006B21122072BF8B5187702@MCINFRWEM003.ucsfmedicalcenter.org> Bernice, I think you are confusing OJT with education requirements. The Education requirements changed, not the route of training. And the education requirements are simply for basic math and science, not specifically for Histotechnology. Since histotech schools are so rare the vast majority of histotechs are still trained on the job. We just hired a person this week who came into the lab several months ago as a temp lab assistant for basic non-histo work and had no clue about histotechnology. However she showed excellent trainability, has a B.S. degree and became very interested in the work we do. We hired her as a full time regular general lab tech (not a histotech) with the intention of training her in to histology. She will qualify to take the HTL in the next couple years. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, May 24, 2012 7:52 AM To: William Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) So why was OJT supposedly off the charts in 2005 (so to speak). Guess not. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: William [mailto:chapcl@yahoo.com] Sent: Thursday, May 24, 2012 9:50 AM To: Bernice Frederick Cc: joelle weaver; lpwenk@sbcglobal.net; tpodawiltz@lrgh.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) OJT is only available to HTL's via the route you described. Sent from my iPhone On May 24, 2012, at 10:45 AM, Bernice Frederick wrote: > If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Thursday, May 24, 2012 9:26 AM > To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > > > Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: lpwenk@sbcglobal.net >> To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org >> CC: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) >> Date: Thu, 24 May 2012 05:43:32 -0400 >> >> I'd like to wade into this discuss with a couple of comments: >> >> LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": >> Yes, I'm still hearing about places like this. When I talk with the >> supervisors, it's because the lab wants the person doing the "histotech" >> job, but they only want to pay them at "lab assistant" wages. Plus, >> once they get the people trained as "histotechs", the employees can't >> go elsewhere, because the other labs only want certified histotech, >> and these people can't get certified as they don't have the associate >> degree and minimum 12 hours of biology and chemistry combined as >> required to take the ASCP HT exam. So these people end up having to >> stay there. (Personally, I think is very unfair to the employees they >> hire.) >> >> LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: >> Even though the High School route was dropped as of Jan 1, 2005 (over >> 7 years ago), I still get emails from labs that want to hire one of >> my students, but their job description says high school diploma. I >> usually call these places up, and the histology supervisor had no >> idea the ASCP HT high school route was dropped. "Someone" should have >> told them. Even though it was in every "NSH in Action" for the 5 >> years previous (that's now over 12 years ago), in some ASCP >> publications each year for the 5 years previous, and on both the NSH >> and ASCP webpage for the 5 years previous, well, since they aren't >> NSH or ASCP members, well, "someone" still should have contacted them directly and let them know. Sigh. >> >> I've had employees call that they were hired after the 2005 deadline, >> with the job description of high school graduate requirement, and >> were told they had 2 years to get the experience required, and then >> they had 1 additional year in which to take and pass the HT exam. And >> when they went to sign up to take the HT exam, they discovered that >> the HT exam requirements had dropped the high school route and now >> the on-the-job (OJT) requires the associate >> degree/60 credit hours with 12 credits of bio/chem, which of course >> they don't have. They tell me that their histology supervisor says >> they are going to fire them, because they can't take the ASCP HT Exam. >> I end up talking with the supervisor, and advise them to talk with >> their HR and Legal departments, as they are the ones who advertised >> the high school requirement, and they are the ones who hired this >> person without the needed education. And I suggest they help with >> person complete an on-line NAACLS HT program, several of which will >> take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. >> >> NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: >> NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, >> but for most lab training programs.) NAACLS has a long list of >> standards for programs to follow. (Think CAP checklist.) >> >> Standard 14 G has a statement "The granting of the degree or >> certificate must not be contingent upon the student's passing any >> type of external certification or licensure examination." >> >> (Explanation: Not all HT programs end in an associate degree. The >> certificate refers to a certificate of completion of a program. My >> program, for example, is hospital-based. Some students already have >> their degree before they start my program. Some have all the college >> credits except for the ones they are earning while completing the >> internship, then they earn their degree from the college when they >> complete the internship and get the grade for those last credit hours. >> The hospital doesn't grant the degree, the college does. The hospital >> program grants a certificate of completion of the program, which is >> acceptable to NAACLS, ASCP, and employers.) >> >> As NAACLS accredited HT or HTL programs, we can encourage our >> students to take the HT/HTL exam upon completion of the program. We >> can do review sessions with them. We can remind them of the deadlines >> to sign up. We can help them sign up if they are having problems. We >> can let them know that labs in our area expect people to be >> certified. We can let them know that they can sign up while still in >> the program (couple of months before graduation), and they can, >> before they graduate, pick a date to take the exam after graduation. >> We can tell them that these dates to take the exams can be put on >> their resume, on the application, and that they can inform the >> supervisor during the interview that they are already signed up to take the HT/HTL exam. >> >> But we can NOT "make" the student take the exam. Completion of the >> program cannot be contingent upon taking or passing the HT/HTL exam >> (or getting state licensure). The program could lose NAACLS >> accreditation if we force the student to take the HT/HTL/state >> licensure exam, or withhold their degree or certificate until they do >> take/pass the HT/HTL exam/become state licensed. >> >> Thanks for "listening". >> >> Peggy A. Wenk, HTL(ASCP)SLS >> Program Director, Schools of Histotechnology William Beaumont >> Hospital Royal Oak, MI 48073 >> >> (The opinions expressed are my own, and do not represent Beaumont >> Hospital.) >> >> -----Original Message----- >> From: joelle weaver >> Sent: Wednesday, May 23, 2012 12:41 PM >> To: tpodawiltz@lrgh.org >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] (no subject) >> >> >> TomI compliment your approach, and I personally really like to see >> when employers do this. I think it provides opportunity, yet >> incentive. Two years seems especially reasonable. Most places that I >> have known who use this method for new-grad hires, have allowed 1 >> year. It is even better when they provide additional training and support to the person as they are preparing. >> Having been involved in an online program and clinical curricula in >> the past, I feel from my observations that continuity with work in >> the environment is essential for most in connecting the theory with execution. >> >> >> >> Joelle Weaver MAOM, HTL (ASCP) QIHC >>> From: tpodawiltz@lrgh.org >>> To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; >>> histonet@lists.utsouthwestern.edu >>> Date: Wed, 23 May 2012 10:45:52 -0400 >>> Subject: RE: [Histonet] (no subject) >>> >>> The last person I hired for a tech position, was just finishing her >>> on-line program. I helped her complete that and then she was given >>> two years to get her certification. This was all part of her hiring >>> agreement, so she knew this coming in and completely understood that >>> failing to achieve either one would affect her employment. >>> >>> >>> >>> Tom Podawiltz HT (ASCP) >>> Histology Section Head/Laboratory Safety Officer. >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>> joelle weaver >>> Sent: Tuesday, May 22, 2012 1:43 PM >>> To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] (no subject) >>> >>> >>> That seems to be the unfortunate situation at this time... >>> >>> >>> >>> >>> Joelle Weaver MAOM, HTL (ASCP) QIHC >>>> Date: Tue, 22 May 2012 12:39:39 -0500 >>>> From: Sherrian.McAnn@va.gov >>>> To: histonet@lists.utsouthwestern.edu >>>> Subject: [Histonet] (no subject) >>>> >>>> I agree and would like to add. This is one scenario that I have >>>> seen many times, where hospitals or wherever will hire histotechs >>>> without certifications . I am thinking that saves them money and >>>> they still have a "histotech". I have seen good histotechs that >>>> have no certification and likewise some bad ones with certification. Lately I >>>> have seen these schools turn out histotechs , it seems with little >>>> encouragement to get certified. If places will hire them without >>>> being certified, there seems little incentive (unless you are self >>>> motivated for more money) to move on up to certification. >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> THIS MESSAGE IS CONFIDENTIAL. >>> This e-mail message and any attachments are proprietary and >>> confidential information intended only for the use of the >>> recipient(s) named above. If you are not the intended recipient, you >>> may not print,distribute, or copy this message or any attachments. >>> If you have received this communication in error, please notify the >>> sender by return e-mail and delete this message and any attachments >>> from your computer. Any views or opinions expressed are solely those >>> of the author and do not necessarily represent those of LRGHealthcare. >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu May 24 11:25:18 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 24 11:25:25 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> <29496B3FDE874BA18B4B3A7A07111F37@HP2010> <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu>, <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> Message-ID: I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. Joelle Weaver MAOM, HTL (ASCP) QIHC > CC: joelleweaver@hotmail.com; lpwenk@sbcglobal.net; tpodawiltz@lrgh.org; histonet@lists.utsouthwestern.edu > From: chapcl@yahoo.com > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > Date: Thu, 24 May 2012 10:50:23 -0400 > To: b-frederick@northwestern.edu > > OJT is only available to HTL's via the route you described. > > Sent from my iPhone > > On May 24, 2012, at 10:45 AM, Bernice Frederick wrote: > > > If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > > Sent: Thursday, May 24, 2012 9:26 AM > > To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > > > > > > Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > >> From: lpwenk@sbcglobal.net > >> To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org > >> CC: histonet@lists.utsouthwestern.edu > >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > >> Date: Thu, 24 May 2012 05:43:32 -0400 > >> > >> I'd like to wade into this discuss with a couple of comments: > >> > >> LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": > >> Yes, I'm still hearing about places like this. When I talk with the > >> supervisors, it's because the lab wants the person doing the "histotech" > >> job, but they only want to pay them at "lab assistant" wages. Plus, > >> once they get the people trained as "histotechs", the employees can't > >> go elsewhere, because the other labs only want certified histotech, > >> and these people can't get certified as they don't have the associate > >> degree and minimum 12 hours of biology and chemistry combined as > >> required to take the ASCP HT exam. So these people end up having to > >> stay there. (Personally, I think is very unfair to the employees they > >> hire.) > >> > >> LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: > >> Even though the High School route was dropped as of Jan 1, 2005 (over > >> 7 years ago), I still get emails from labs that want to hire one of > >> my students, but their job description says high school diploma. I > >> usually call these places up, and the histology supervisor had no idea > >> the ASCP HT high school route was dropped. "Someone" should have told > >> them. Even though it was in every "NSH in Action" for the 5 years > >> previous (that's now over 12 years ago), in some ASCP publications > >> each year for the 5 years previous, and on both the NSH and ASCP > >> webpage for the 5 years previous, well, since they aren't NSH or ASCP > >> members, well, "someone" still should have contacted them directly and let them know. Sigh. > >> > >> I've had employees call that they were hired after the 2005 deadline, > >> with the job description of high school graduate requirement, and were > >> told they had 2 years to get the experience required, and then they > >> had 1 additional year in which to take and pass the HT exam. And when > >> they went to sign up to take the HT exam, they discovered that the HT > >> exam requirements had dropped the high school route and now the > >> on-the-job (OJT) requires the associate > >> degree/60 credit hours with 12 credits of bio/chem, which of course > >> they don't have. They tell me that their histology supervisor says > >> they are going to fire them, because they can't take the ASCP HT Exam. > >> I end up talking with the supervisor, and advise them to talk with > >> their HR and Legal departments, as they are the ones who advertised > >> the high school requirement, and they are the ones who hired this > >> person without the needed education. And I suggest they help with > >> person complete an on-line NAACLS HT program, several of which will > >> take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. > >> > >> NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: > >> NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, > >> but for most lab training programs.) NAACLS has a long list of > >> standards for programs to follow. (Think CAP checklist.) > >> > >> Standard 14 G has a statement "The granting of the degree or > >> certificate must not be contingent upon the student's passing any type > >> of external certification or licensure examination." > >> > >> (Explanation: Not all HT programs end in an associate degree. The > >> certificate refers to a certificate of completion of a program. My > >> program, for example, is hospital-based. Some students already have > >> their degree before they start my program. Some have all the college > >> credits except for the ones they are earning while completing the > >> internship, then they earn their degree from the college when they > >> complete the internship and get the grade for those last credit hours. > >> The hospital doesn't grant the degree, the college does. The hospital > >> program grants a certificate of completion of the program, which is > >> acceptable to NAACLS, ASCP, and employers.) > >> > >> As NAACLS accredited HT or HTL programs, we can encourage our students > >> to take the HT/HTL exam upon completion of the program. We can do > >> review sessions with them. We can remind them of the deadlines to sign > >> up. We can help them sign up if they are having problems. We can let > >> them know that labs in our area expect people to be certified. We can > >> let them know that they can sign up while still in the program (couple > >> of months before graduation), and they can, before they graduate, pick > >> a date to take the exam after graduation. We can tell them that these > >> dates to take the exams can be put on their resume, on the > >> application, and that they can inform the supervisor during the > >> interview that they are already signed up to take the HT/HTL exam. > >> > >> But we can NOT "make" the student take the exam. Completion of the > >> program cannot be contingent upon taking or passing the HT/HTL exam > >> (or getting state licensure). The program could lose NAACLS > >> accreditation if we force the student to take the HT/HTL/state > >> licensure exam, or withhold their degree or certificate until they do > >> take/pass the HT/HTL exam/become state licensed. > >> > >> Thanks for "listening". > >> > >> Peggy A. Wenk, HTL(ASCP)SLS > >> Program Director, Schools of Histotechnology William Beaumont Hospital > >> Royal Oak, MI 48073 > >> > >> (The opinions expressed are my own, and do not represent Beaumont > >> Hospital.) > >> > >> -----Original Message----- > >> From: joelle weaver > >> Sent: Wednesday, May 23, 2012 12:41 PM > >> To: tpodawiltz@lrgh.org > >> Cc: histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] (no subject) > >> > >> > >> TomI compliment your approach, and I personally really like to see > >> when employers do this. I think it provides opportunity, yet > >> incentive. Two years seems especially reasonable. Most places that I > >> have known who use this method for new-grad hires, have allowed 1 > >> year. It is even better when they provide additional training and support to the person as they are preparing. > >> Having been involved in an online program and clinical curricula in > >> the past, I feel from my observations that continuity with work in the > >> environment is essential for most in connecting the theory with execution. > >> > >> > >> > >> Joelle Weaver MAOM, HTL (ASCP) QIHC > >>> From: tpodawiltz@lrgh.org > >>> To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > >>> histonet@lists.utsouthwestern.edu > >>> Date: Wed, 23 May 2012 10:45:52 -0400 > >>> Subject: RE: [Histonet] (no subject) > >>> > >>> The last person I hired for a tech position, was just finishing her > >>> on-line program. I helped her complete that and then she was given > >>> two years to get her certification. This was all part of her hiring > >>> agreement, so she knew this coming in and completely understood that > >>> failing to achieve either one would affect her employment. > >>> > >>> > >>> > >>> Tom Podawiltz HT (ASCP) > >>> Histology Section Head/Laboratory Safety Officer. > >>> > >>> > >>> > >>> > >>> > >>> -----Original Message----- > >>> From: histonet-bounces@lists.utsouthwestern.edu > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >>> joelle weaver > >>> Sent: Tuesday, May 22, 2012 1:43 PM > >>> To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > >>> Subject: RE: [Histonet] (no subject) > >>> > >>> > >>> That seems to be the unfortunate situation at this time... > >>> > >>> > >>> > >>> > >>> Joelle Weaver MAOM, HTL (ASCP) QIHC > >>>> Date: Tue, 22 May 2012 12:39:39 -0500 > >>>> From: Sherrian.McAnn@va.gov > >>>> To: histonet@lists.utsouthwestern.edu > >>>> Subject: [Histonet] (no subject) > >>>> > >>>> I agree and would like to add. This is one scenario that I have > >>>> seen many times, where hospitals or wherever will hire histotechs > >>>> without certifications . I am thinking that saves them money and > >>>> they still have a "histotech". I have seen good histotechs that > >>>> have no certification and likewise some bad ones with certification. Lately I > >>>> have seen these schools turn out histotechs , it seems with little > >>>> encouragement to get certified. If places will hire them without > >>>> being certified, there seems little incentive (unless you are > >>>> self motivated for more money) to move on up to certification. > >>>> > >>>> > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> THIS MESSAGE IS CONFIDENTIAL. > >>> This e-mail message and any attachments are proprietary and > >>> confidential information intended only for the use of the > >>> recipient(s) named above. If you are not the intended recipient, you > >>> may not print,distribute, or copy this message or any attachments. > >>> If you have received this communication in error, please notify the > >>> sender by return e-mail and delete this message and any attachments > >>> from your computer. Any views or opinions expressed are solely those > >>> of the author and do not necessarily represent those of LRGHealthcare. > >>> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkrupp <@t> deltacollege.edu Thu May 24 11:53:21 2012 From: jkrupp <@t> deltacollege.edu (Jon Krupp) Date: Thu May 24 11:53:29 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> <29496B3FDE874BA18B4B3A7A07111F37@HP2010> <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu>, <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> Message-ID: <873934A8-E729-4E82-9D1A-77ED6E5660B0@deltacollege.edu> On May 24, 2012, at 9:25 AM, joelle weaver wrote: > > I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. OK, so I have a question. We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College From Timothy.Morken <@t> ucsfmedctr.org Thu May 24 11:56:25 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 24 11:56:23 2012 Subject: [Histonet] RE: Not requiring HT Certification Message-ID: <8D7C2D242DBD45498006B21122072BF8B518776A@MCINFRWEM003.ucsfmedicalcenter.org> " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 24, 2012 9:25 AM To: chapcl@yahoo.com; b-frederick@northwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. Joelle Weaver MAOM, HTL (ASCP) QIHC > CC: joelleweaver@hotmail.com; lpwenk@sbcglobal.net; tpodawiltz@lrgh.org; histonet@lists.utsouthwestern.edu > From: chapcl@yahoo.com > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > Date: Thu, 24 May 2012 10:50:23 -0400 > To: b-frederick@northwestern.edu > > OJT is only available to HTL's via the route you described. > > Sent from my iPhone > > On May 24, 2012, at 10:45 AM, Bernice Frederick wrote: > > > If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > joelle weaver > > Sent: Thursday, May 24, 2012 9:26 AM > > To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] (no subject) (Not requiring HT > > Certification) > > > > > > Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > >> From: lpwenk@sbcglobal.net > >> To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org > >> CC: histonet@lists.utsouthwestern.edu > >> Subject: Re: [Histonet] (no subject) (Not requiring HT > >> Certification) > >> Date: Thu, 24 May 2012 05:43:32 -0400 > >> > >> I'd like to wade into this discuss with a couple of comments: > >> > >> LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": > >> Yes, I'm still hearing about places like this. When I talk with the > >> supervisors, it's because the lab wants the person doing the "histotech" > >> job, but they only want to pay them at "lab assistant" wages. Plus, > >> once they get the people trained as "histotechs", the employees > >> can't go elsewhere, because the other labs only want certified > >> histotech, and these people can't get certified as they don't have > >> the associate degree and minimum 12 hours of biology and chemistry > >> combined as required to take the ASCP HT exam. So these people end > >> up having to stay there. (Personally, I think is very unfair to the > >> employees they > >> hire.) > >> > >> LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: > >> Even though the High School route was dropped as of Jan 1, 2005 > >> (over > >> 7 years ago), I still get emails from labs that want to hire one > >> of my students, but their job description says high school diploma. > >> I usually call these places up, and the histology supervisor had no > >> idea the ASCP HT high school route was dropped. "Someone" should > >> have told them. Even though it was in every "NSH in Action" for the > >> 5 years previous (that's now over 12 years ago), in some ASCP > >> publications each year for the 5 years previous, and on both the > >> NSH and ASCP webpage for the 5 years previous, well, since they > >> aren't NSH or ASCP members, well, "someone" still should have contacted them directly and let them know. Sigh. > >> > >> I've had employees call that they were hired after the 2005 > >> deadline, with the job description of high school graduate > >> requirement, and were told they had 2 years to get the experience > >> required, and then they had 1 additional year in which to take and > >> pass the HT exam. And when they went to sign up to take the HT > >> exam, they discovered that the HT exam requirements had dropped the > >> high school route and now the on-the-job (OJT) requires the > >> associate > >> degree/60 credit hours with 12 credits of bio/chem, which of course > >> they don't have. They tell me that their histology supervisor says > >> they are going to fire them, because they can't take the ASCP HT Exam. > >> I end up talking with the supervisor, and advise them to talk with > >> their HR and Legal departments, as they are the ones who advertised > >> the high school requirement, and they are the ones who hired this > >> person without the needed education. And I suggest they help with > >> person complete an on-line NAACLS HT program, several of which will > >> take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. > >> > >> NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: > >> NAACLS is the accrediting agency for HT and HTL programs. (Think > >> CAP, but for most lab training programs.) NAACLS has a long list of > >> standards for programs to follow. (Think CAP checklist.) > >> > >> Standard 14 G has a statement "The granting of the degree or > >> certificate must not be contingent upon the student's passing any > >> type of external certification or licensure examination." > >> > >> (Explanation: Not all HT programs end in an associate degree. The > >> certificate refers to a certificate of completion of a program. My > >> program, for example, is hospital-based. Some students already have > >> their degree before they start my program. Some have all the > >> college credits except for the ones they are earning while > >> completing the internship, then they earn their degree from the > >> college when they complete the internship and get the grade for those last credit hours. > >> The hospital doesn't grant the degree, the college does. The > >> hospital program grants a certificate of completion of the program, > >> which is acceptable to NAACLS, ASCP, and employers.) > >> > >> As NAACLS accredited HT or HTL programs, we can encourage our > >> students to take the HT/HTL exam upon completion of the program. We > >> can do review sessions with them. We can remind them of the > >> deadlines to sign up. We can help them sign up if they are having > >> problems. We can let them know that labs in our area expect people > >> to be certified. We can let them know that they can sign up while > >> still in the program (couple of months before graduation), and they > >> can, before they graduate, pick a date to take the exam after > >> graduation. We can tell them that these dates to take the exams can > >> be put on their resume, on the application, and that they can > >> inform the supervisor during the interview that they are already signed up to take the HT/HTL exam. > >> > >> But we can NOT "make" the student take the exam. Completion of the > >> program cannot be contingent upon taking or passing the HT/HTL exam > >> (or getting state licensure). The program could lose NAACLS > >> accreditation if we force the student to take the HT/HTL/state > >> licensure exam, or withhold their degree or certificate until they > >> do take/pass the HT/HTL exam/become state licensed. > >> > >> Thanks for "listening". > >> > >> Peggy A. Wenk, HTL(ASCP)SLS > >> Program Director, Schools of Histotechnology William Beaumont > >> Hospital Royal Oak, MI 48073 > >> > >> (The opinions expressed are my own, and do not represent Beaumont > >> Hospital.) > >> > >> -----Original Message----- > >> From: joelle weaver > >> Sent: Wednesday, May 23, 2012 12:41 PM > >> To: tpodawiltz@lrgh.org > >> Cc: histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] (no subject) > >> > >> > >> TomI compliment your approach, and I personally really like to see > >> when employers do this. I think it provides opportunity, yet > >> incentive. Two years seems especially reasonable. Most places that > >> I have known who use this method for new-grad hires, have allowed 1 > >> year. It is even better when they provide additional training and support to the person as they are preparing. > >> Having been involved in an online program and clinical curricula in > >> the past, I feel from my observations that continuity with work in > >> the environment is essential for most in connecting the theory with execution. > >> > >> > >> > >> Joelle Weaver MAOM, HTL (ASCP) QIHC > >>> From: tpodawiltz@lrgh.org > >>> To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > >>> histonet@lists.utsouthwestern.edu > >>> Date: Wed, 23 May 2012 10:45:52 -0400 > >>> Subject: RE: [Histonet] (no subject) > >>> > >>> The last person I hired for a tech position, was just finishing > >>> her on-line program. I helped her complete that and then she was > >>> given two years to get her certification. This was all part of her > >>> hiring agreement, so she knew this coming in and completely > >>> understood that failing to achieve either one would affect her employment. > >>> > >>> > >>> > >>> Tom Podawiltz HT (ASCP) > >>> Histology Section Head/Laboratory Safety Officer. > >>> > >>> > >>> > >>> > >>> > >>> -----Original Message----- > >>> From: histonet-bounces@lists.utsouthwestern.edu > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >>> joelle weaver > >>> Sent: Tuesday, May 22, 2012 1:43 PM > >>> To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > >>> Subject: RE: [Histonet] (no subject) > >>> > >>> > >>> That seems to be the unfortunate situation at this time... > >>> > >>> > >>> > >>> > >>> Joelle Weaver MAOM, HTL (ASCP) QIHC > >>>> Date: Tue, 22 May 2012 12:39:39 -0500 > >>>> From: Sherrian.McAnn@va.gov > >>>> To: histonet@lists.utsouthwestern.edu > >>>> Subject: [Histonet] (no subject) > >>>> > >>>> I agree and would like to add. This is one scenario that I have > >>>> seen many times, where hospitals or wherever will hire > >>>> histotechs without certifications . I am thinking that saves > >>>> them money and they still have a "histotech". I have seen good > >>>> histotechs that have no certification and likewise some bad ones with certification. Lately I > >>>> have seen these schools turn out histotechs , it seems with little > >>>> encouragement to get certified. If places will hire them without > >>>> being certified, there seems little incentive (unless you are > >>>> self motivated for more money) to move on up to certification. > >>>> > >>>> > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> THIS MESSAGE IS CONFIDENTIAL. > >>> This e-mail message and any attachments are proprietary and > >>> confidential information intended only for the use of the > >>> recipient(s) named above. If you are not the intended recipient, > >>> you may not print,distribute, or copy this message or any attachments. > >>> If you have received this communication in error, please notify > >>> the sender by return e-mail and delete this message and any > >>> attachments from your computer. Any views or opinions expressed > >>> are solely those of the author and do not necessarily represent those of LRGHealthcare. > >>> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu May 24 12:20:41 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 24 12:20:45 2012 Subject: [Histonet] (no subject) (Not requiring HT Certification) In-Reply-To: <873934A8-E729-4E82-9D1A-77ED6E5660B0@deltacollege.edu> References: <61E2B58CECEF384094A363989D47C090084E5A13@VHAV17MSGA2.v17.med.va.gov> <38667E7FB77ECD4E91BFAEB8D986386324FBA81CD5@LRGHEXVS1.practice.lrgh.org> <29496B3FDE874BA18B4B3A7A07111F37@HP2010> <62C639732D3F274DACED033EBDF6ADAF1E252145@evcspmbx3.ads.northwestern.edu>, <7A00EB5F-84F5-441B-82F2-00682BFE2A69@yahoo.com> , <873934A8-E729-4E82-9D1A-77ED6E5660B0@deltacollege.edu> Message-ID: Jon There is a route with associates and training I believe. Of course I can't speak for the BOC, and I am sure that you want to help your employees as much as you can. I do see your point about the similarities in tasks. My thought would be that the exam eligibility states that they have to have recent experience in fixation, embedding, microtomy, and staining (histology) and the associated theory knowledge. EM is on the exam study topics, but also with the theory/experience for all those routine histological techniques, is how I read it. Take a look at the exam outlines, that should give you an idea of the scope. Ascp.org "get certified". As I have been told, they want to cover the widest possible scope of roles histologists can perform, which could include EM, but not only that. If they don't have exposure to regular histology I think that it might be hard for to feel prepared for the regular HT or HTL exams. That's just my opinion, based on what I have observed and also the pass rates ( ~ 65%), for people even with training/experience- there could be an exceptional person out there. I can understand not wanting to get buried in doing a whole HT curricula ( believe me, I do). How about the option of having cross training in a histology lab? Do you have routine histology on site or a nearby lab? The best advice I can give is to go to the website and carefully read the requirments to see how your employees might fit in. If you want to provide the theory without having to do the curricula, there are on line programs out there which can supplement OJT and a supportive mentor and organization. I have seen this work successfully with motivated people with the ability to have hands on practice alongside. I suggest the NSH site which lists the accredited programs or the NAACLS site which has a search for programs, if that would help. As far as employability, my opinion is that it would certainly open up options for your employees to also have skills in routine histology make them more valuable to your organization, and I would think certification would be even more helpful to them as far as options. Joelle Weaver MAOM, HTL (ASCP) QIHC > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > From: jkrupp@deltacollege.edu > Date: Thu, 24 May 2012 09:53:21 -0700 > CC: histonet@lists.utsouthwestern.edu > To: joelleweaver@hotmail.com > > > On May 24, 2012, at 9:25 AM, joelle weaver wrote: > > > > > I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. > > OK, so I have a question. > > We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. > > So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? > > Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > From joelleweaver <@t> hotmail.com Thu May 24 12:28:10 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 24 12:28:19 2012 Subject: [Histonet] RE: Not requiring HT Certification In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B518776A@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8B518776A@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Yes I was referring to the HS and OJT training route. There is confusion about the degree + OJT and HS + OJT. Yes, that was my understanding also of the drivers, I just could not recall where I read/heard that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 24 May 2012 09:56:25 -0700 > Subject: [Histonet] RE: Not requiring HT Certification > > " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." > > Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. > > If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. > > > Tim Morken > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, May 24, 2012 9:25 AM > To: chapcl@yahoo.com; b-frederick@northwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > > > I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > CC: joelleweaver@hotmail.com; lpwenk@sbcglobal.net; tpodawiltz@lrgh.org; histonet@lists.utsouthwestern.edu > > From: chapcl@yahoo.com > > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > > Date: Thu, 24 May 2012 10:50:23 -0400 > > To: b-frederick@northwestern.edu > > > > OJT is only available to HTL's via the route you described. > > > > Sent from my iPhone > > > > On May 24, 2012, at 10:45 AM, Bernice Frederick wrote: > > > > > If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. > > > Bernice > > > > > > Bernice Frederick HTL (ASCP) > > > Senior Research Tech > > > Pathology Core Facility > > > ECOGPCO-RL > > > Robert. H. Lurie Cancer Center > > > Northwestern University > > > 710 N Fairbanks Court > > > Olson 8-421 > > > Chicago,IL 60611 > > > 312-503-3723 > > > b-frederick@northwestern.edu > > > > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > > joelle weaver > > > Sent: Thursday, May 24, 2012 9:26 AM > > > To: lpwenk@sbcglobal.net; tpodawiltz@lrgh.org > > > Cc: histonet@lists.utsouthwestern.edu > > > Subject: RE: [Histonet] (no subject) (Not requiring HT > > > Certification) > > > > > > > > > Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. > > > > > > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > >> From: lpwenk@sbcglobal.net > > >> To: joelleweaver@hotmail.com; tpodawiltz@lrgh.org > > >> CC: histonet@lists.utsouthwestern.edu > > >> Subject: Re: [Histonet] (no subject) (Not requiring HT > > >> Certification) > > >> Date: Thu, 24 May 2012 05:43:32 -0400 > > >> > > >> I'd like to wade into this discuss with a couple of comments: > > >> > > >> LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED "HISTOTECHS": > > >> Yes, I'm still hearing about places like this. When I talk with the > > >> supervisors, it's because the lab wants the person doing the "histotech" > > >> job, but they only want to pay them at "lab assistant" wages. Plus, > > >> once they get the people trained as "histotechs", the employees > > >> can't go elsewhere, because the other labs only want certified > > >> histotech, and these people can't get certified as they don't have > > >> the associate degree and minimum 12 hours of biology and chemistry > > >> combined as required to take the ASCP HT exam. So these people end > > >> up having to stay there. (Personally, I think is very unfair to the > > >> employees they > > >> hire.) > > >> > > >> LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: > > >> Even though the High School route was dropped as of Jan 1, 2005 > > >> (over > > >> 7 years ago), I still get emails from labs that want to hire one > > >> of my students, but their job description says high school diploma. > > >> I usually call these places up, and the histology supervisor had no > > >> idea the ASCP HT high school route was dropped. "Someone" should > > >> have told them. Even though it was in every "NSH in Action" for the > > >> 5 years previous (that's now over 12 years ago), in some ASCP > > >> publications each year for the 5 years previous, and on both the > > >> NSH and ASCP webpage for the 5 years previous, well, since they > > >> aren't NSH or ASCP members, well, "someone" still should have contacted them directly and let them know. Sigh. > > >> > > >> I've had employees call that they were hired after the 2005 > > >> deadline, with the job description of high school graduate > > >> requirement, and were told they had 2 years to get the experience > > >> required, and then they had 1 additional year in which to take and > > >> pass the HT exam. And when they went to sign up to take the HT > > >> exam, they discovered that the HT exam requirements had dropped the > > >> high school route and now the on-the-job (OJT) requires the > > >> associate > > >> degree/60 credit hours with 12 credits of bio/chem, which of course > > >> they don't have. They tell me that their histology supervisor says > > >> they are going to fire them, because they can't take the ASCP HT Exam. > > >> I end up talking with the supervisor, and advise them to talk with > > >> their HR and Legal departments, as they are the ones who advertised > > >> the high school requirement, and they are the ones who hired this > > >> person without the needed education. And I suggest they help with > > >> person complete an on-line NAACLS HT program, several of which will > > >> take someone with the high school diploma, as long as they had a biology, a chemistry, and a math class in high school. > > >> > > >> NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM: > > >> NAACLS is the accrediting agency for HT and HTL programs. (Think > > >> CAP, but for most lab training programs.) NAACLS has a long list of > > >> standards for programs to follow. (Think CAP checklist.) > > >> > > >> Standard 14 G has a statement "The granting of the degree or > > >> certificate must not be contingent upon the student's passing any > > >> type of external certification or licensure examination." > > >> > > >> (Explanation: Not all HT programs end in an associate degree. The > > >> certificate refers to a certificate of completion of a program. My > > >> program, for example, is hospital-based. Some students already have > > >> their degree before they start my program. Some have all the > > >> college credits except for the ones they are earning while > > >> completing the internship, then they earn their degree from the > > >> college when they complete the internship and get the grade for those last credit hours. > > >> The hospital doesn't grant the degree, the college does. The > > >> hospital program grants a certificate of completion of the program, > > >> which is acceptable to NAACLS, ASCP, and employers.) > > >> > > >> As NAACLS accredited HT or HTL programs, we can encourage our > > >> students to take the HT/HTL exam upon completion of the program. We > > >> can do review sessions with them. We can remind them of the > > >> deadlines to sign up. We can help them sign up if they are having > > >> problems. We can let them know that labs in our area expect people > > >> to be certified. We can let them know that they can sign up while > > >> still in the program (couple of months before graduation), and they > > >> can, before they graduate, pick a date to take the exam after > > >> graduation. We can tell them that these dates to take the exams can > > >> be put on their resume, on the application, and that they can > > >> inform the supervisor during the interview that they are already signed up to take the HT/HTL exam. > > >> > > >> But we can NOT "make" the student take the exam. Completion of the > > >> program cannot be contingent upon taking or passing the HT/HTL exam > > >> (or getting state licensure). The program could lose NAACLS > > >> accreditation if we force the student to take the HT/HTL/state > > >> licensure exam, or withhold their degree or certificate until they > > >> do take/pass the HT/HTL exam/become state licensed. > > >> > > >> Thanks for "listening". > > >> > > >> Peggy A. Wenk, HTL(ASCP)SLS > > >> Program Director, Schools of Histotechnology William Beaumont > > >> Hospital Royal Oak, MI 48073 > > >> > > >> (The opinions expressed are my own, and do not represent Beaumont > > >> Hospital.) > > >> > > >> -----Original Message----- > > >> From: joelle weaver > > >> Sent: Wednesday, May 23, 2012 12:41 PM > > >> To: tpodawiltz@lrgh.org > > >> Cc: histonet@lists.utsouthwestern.edu > > >> Subject: RE: [Histonet] (no subject) > > >> > > >> > > >> TomI compliment your approach, and I personally really like to see > > >> when employers do this. I think it provides opportunity, yet > > >> incentive. Two years seems especially reasonable. Most places that > > >> I have known who use this method for new-grad hires, have allowed 1 > > >> year. It is even better when they provide additional training and support to the person as they are preparing. > > >> Having been involved in an online program and clinical curricula in > > >> the past, I feel from my observations that continuity with work in > > >> the environment is essential for most in connecting the theory with execution. > > >> > > >> > > >> > > >> Joelle Weaver MAOM, HTL (ASCP) QIHC > > >>> From: tpodawiltz@lrgh.org > > >>> To: joelleweaver@hotmail.com; sherrian.mcann@va.gov; > > >>> histonet@lists.utsouthwestern.edu > > >>> Date: Wed, 23 May 2012 10:45:52 -0400 > > >>> Subject: RE: [Histonet] (no subject) > > >>> > > >>> The last person I hired for a tech position, was just finishing > > >>> her on-line program. I helped her complete that and then she was > > >>> given two years to get her certification. This was all part of her > > >>> hiring agreement, so she knew this coming in and completely > > >>> understood that failing to achieve either one would affect her employment. > > >>> > > >>> > > >>> > > >>> Tom Podawiltz HT (ASCP) > > >>> Histology Section Head/Laboratory Safety Officer. > > >>> > > >>> > > >>> > > >>> > > >>> > > >>> -----Original Message----- > > >>> From: histonet-bounces@lists.utsouthwestern.edu > > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > >>> joelle weaver > > >>> Sent: Tuesday, May 22, 2012 1:43 PM > > >>> To: sherrian.mcann@va.gov; histonet@lists.utsouthwestern.edu > > >>> Subject: RE: [Histonet] (no subject) > > >>> > > >>> > > >>> That seems to be the unfortunate situation at this time... > > >>> > > >>> > > >>> > > >>> > > >>> Joelle Weaver MAOM, HTL (ASCP) QIHC > > >>>> Date: Tue, 22 May 2012 12:39:39 -0500 > > >>>> From: Sherrian.McAnn@va.gov > > >>>> To: histonet@lists.utsouthwestern.edu > > >>>> Subject: [Histonet] (no subject) > > >>>> > > >>>> I agree and would like to add. This is one scenario that I have > > >>>> seen many times, where hospitals or wherever will hire > > >>>> histotechs without certifications . I am thinking that saves > > >>>> them money and they still have a "histotech". I have seen good > > >>>> histotechs that have no certification and likewise some bad ones with certification. Lately I > > >>>> have seen these schools turn out histotechs , it seems with little > > >>>> encouragement to get certified. If places will hire them without > > >>>> being certified, there seems little incentive (unless you are > > >>>> self motivated for more money) to move on up to certification. > > >>>> > > >>>> > > >>>> > > >>>> _______________________________________________ > > >>>> Histonet mailing list > > >>>> Histonet@lists.utsouthwestern.edu > > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >>> _______________________________________________ > > >>> Histonet mailing list > > >>> Histonet@lists.utsouthwestern.edu > > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >>> THIS MESSAGE IS CONFIDENTIAL. > > >>> This e-mail message and any attachments are proprietary and > > >>> confidential information intended only for the use of the > > >>> recipient(s) named above. If you are not the intended recipient, > > >>> you may not print,distribute, or copy this message or any attachments. > > >>> If you have received this communication in error, please notify > > >>> the sender by return e-mail and delete this message and any > > >>> attachments from your computer. Any views or opinions expressed > > >>> are solely those of the author and do not necessarily represent those of LRGHealthcare. > > >>> > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From virginiachladek <@t> wpuafl.com Thu May 24 12:36:10 2012 From: virginiachladek <@t> wpuafl.com (Virginia Chladek) Date: Thu May 24 12:38:06 2012 Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 20 References: Message-ID: <9BB0A278800B0E41BBC28883FFAB45352E1C22@exchsvr.wpu.local> Hi all- Can someone help me with a billing question- We do a Giemsa stain maybe once a year, and I can't remember how we billed for it. Any ideas? Thank you! Maggie HTL, Winter Park, Florida From jaylundgren <@t> gmail.com Thu May 24 13:40:52 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu May 24 13:40:56 2012 Subject: [Histonet] Unregistered techs Message-ID: Question 1: Why do health care organizations NOT hire unlicensed Physicians? Answer: Because of the maelstrom of malpractice suits, civil and criminal laws they would be breaking, ruined careers, prison, orphaned children etc. Question 2: Why do supervisors/lab managers hire "unregistered" (see previous posts) histotechs? Answer: Because they can pay them $12/hr instead of $30/hr. Many of them receive hefty bonuses for controlling costs. They get away with it. The most brilliant Pathologist in the world could NEVER EVEN SEE a malignancy that was rough cut away by an unqualified histotech. Jay A. Lundgren, M.S., HTL(ASCP) From joelleweaver <@t> hotmail.com Thu May 24 13:49:48 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 24 13:49:52 2012 Subject: [Histonet] RE: Microwave verification In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB34915@CVMMB02.cvm.tamu.edu>, Message-ID: Try the CLSI guideline. It is being revised now, but the original is pretty detailed. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tajibade@echd.org > To: LBUSTAMANTE@cvm.tamu.edu; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > Date: Wed, 23 May 2012 14:33:33 -0500 > CC: > Subject: [Histonet] RE: Microwave verification > > Can you please give more details about this question? > > Tunde Ajibade BS, HTL(ASCP)QIHC > Histology Supervisor > Medical Center Hospital > Odessa,TX > Tel: 432-640-2348 > Fax:432-640-2303 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin > Sent: Wednesday, May 23, 2012 1:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microwave verification > > Does anyone knows a protocol for Microwave verification? (CAP requirement). > Thank you. > Lin Bustamante > Central Texas Gastrointestinal Clinic > Histology laboratory. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu May 24 13:52:07 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu May 24 13:52:10 2012 Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) In-Reply-To: <1337871437.63943.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <14C3108E8B98EF43B3EDAE5833670034152E7A@exchange.med.mun.ca> <1337871437.63943.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: Sadly, I've worked at dozens of labs where they pour it down the sink. I have actually heard Technical Representatives/ Salesmen tell prospective purchasers of their immunostainers that the waste was safe to pour down the sink. Most labs will follow the cheapest path they can get away with, based on their municipality's water regulations, or their own ignorance of said regulation. Dilution does not equal neutralization. We are putting metric tons of DAB into our watershed, milliliter by milliliter. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From cindy38017 <@t> yahoo.com Thu May 24 14:01:39 2012 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Thu May 24 14:01:42 2012 Subject: [Histonet] embedding Message-ID: <1337886099.6871.YahooMailClassic@web37101.mail.mud.yahoo.com> On average, how many blocks should a tech with 6 years experience be able to embed in an hour? This is a dermpath lab, where the majority of our specimens are shave and punch biopsies. Thanks, Cindy From rjbuesa <@t> yahoo.com Thu May 24 14:55:45 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 24 14:55:50 2012 Subject: [Histonet] embedding In-Reply-To: <1337886099.6871.YahooMailClassic@web37101.mail.mud.yahoo.com> Message-ID: <1337889345.61986.YahooMailClassic@web162103.mail.bf1.yahoo.com> 60 Ren? J. --- On Thu, 5/24/12, cindy dewar wrote: From: cindy dewar Subject: [Histonet] embedding To: histonet@lists.utsouthwestern.edu Date: Thursday, May 24, 2012, 3:01 PM On average, how many blocks should a tech with 6 years experience be able to embed in an hour? This is a dermpath lab, where the majority of our specimens are shave and punch biopsies. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu May 24 15:14:48 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu May 24 15:16:47 2012 Subject: [Histonet] embedding In-Reply-To: <1337889345.61986.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <1337886099.6871.YahooMailClassic@web37101.mail.mud.yahoo.com>, <1337889345.61986.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: If you are embedding skins and they all have to be on edge. And some of them are bisected or tri-sected and you have to put two to three pieces of skin in one cassette all on edge. I would say a good tech could do about 45-50 per hour. If they were all punches, then 60 would be about right. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] Sent: Thursday, May 24, 2012 3:55 PM To: histonet@lists.utsouthwestern.edu; cindy dewar Subject: Re: [Histonet] embedding 60 Ren? J. --- On Thu, 5/24/12, cindy dewar wrote: From: cindy dewar Subject: [Histonet] embedding To: histonet@lists.utsouthwestern.edu Date: Thursday, May 24, 2012, 3:01 PM On average, how many blocks should a tech with 6 years experience be able to embed in an hour? This is a dermpath lab, where the majority of our specimens are shave and punch biopsies. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Thu May 24 15:44:33 2012 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Thu May 24 15:44:39 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: Message-ID: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Ok. I have to chime in on this one. Jay, I see your point. I'm neutral on this subject. I work with an unregistered tech (30yrs on the bench) and her work is excellent. For whatever reason she missed the dead line for the change on the OJT route on entry. I have worked with registered techs out there that do crappy work. So what is the difference between registered vs unregistered when it comes to their skills? Unregistered doesn't necessarily mean unqualified. Sent from the iPhone of Kim Tournear. On May 24, 2012, at 1:40 PM, Jay Lundgren wrote: > Question 1: Why do health care organizations NOT hire unlicensed Physicians? > Answer: Because of the maelstrom of malpractice suits, civil and criminal > laws they would be breaking, ruined careers, prison, orphaned children etc. > > Question 2: Why do supervisors/lab managers hire "unregistered" (see > previous posts) histotechs? > > Answer: Because they can pay them $12/hr instead of $30/hr. > Many of them receive hefty bonuses for controlling costs. > They get away with it. > > The most brilliant Pathologist in the world could NEVER EVEN SEE a > malignancy that was rough cut away by an unqualified histotech. > > Jay A. > Lundgren, M.S., HTL(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu May 24 16:01:46 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu May 24 16:01:49 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: Scott Lyons slnj07@yahoo.com Give me a break, HTs and HTLs do not make diagnoses or treat patients. I am a registered HT and a Florida licensed HTL with 19 years experience, I've done it all in the lab. I believe the certification and licensure of techs is a scam to bleed more money from people. Honestly, you can train a monkey to do our job. And I don't want to hear from everyone saying it's an art form, we are just as much needed as pathologists, blah, blah, blah....... I work where they are hiring people from a masters degree program for histology with certification, THEY KNOW NOTHING. Experience it where it's at, whether certified or not, get off your high horse. > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Thu May 24 17:01:30 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Thu May 24 17:01:34 2012 Subject: [Histonet] Unregistered techs Message-ID: <8067136403215664900@unknownmsgid> Oh someone is going to get BLASTED and I'm so glad it's not me this time! But I have to say.... Shame shame for suggesting a monkey can do that job. Doesn't speak well of your work, but most techs I know are very talented. I can't do their work, and I like to think I am a little more evolved than a monkey. At least an ape for crying out loud! Sent from my Windows Phone From: Jay Lundgren Sent: 5/24/2012 2:02 PM To: Kim Tournear Cc: histonet Subject: Re: [Histonet] Unregistered techs Scott Lyons slnj07@yahoo.com Give me a break, HTs and HTLs do not make diagnoses or treat patients. I am a registered HT and a Florida licensed HTL with 19 years experience, I've done it all in the lab. I believe the certification and licensure of techs is a scam to bleed more money from people. Honestly, you can train a monkey to do our job. And I don't want to hear from everyone saying it's an art form, we are just as much needed as pathologists, blah, blah, blah....... I work where they are hiring people from a masters degree program for histology with certification, THEY KNOW NOTHING. Experience it where it's at, whether certified or not, get off your high horse. > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Thu May 24 17:48:23 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Thu May 24 17:48:28 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: I'm sorry - I cannot let this rest. The comment: "we are just as much needed as pathologists, blah, blah, blah......." is so upsetting I cannot sit back and listen to that without saying something! Everyone, regardless of their lot in life, is a very worthwhile part of the whole. Let me ask you a question, since you highly undervalue humans that are not MD's - let's say that you are a patient at Hospital X, and you go in to have your toenail removed. Who plays a more important role in your survival - the Podiatrist or the hospital janitor? I would argue that the janitor is more crucial in this instance, for if he/she fails to clean up the MRSA from the last patient you could conceivably die. The doctor solved your fungal problem, but the janitor prevented you from getting a potentially life-threatening infection. Think before you speak like that - everyone involved in your care is critical - and, yes, sometimes the doctor is not the most important person when it comes to keeping you alive and well! On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren wrote: > Scott Lyons slnj07@yahoo.com > > Give me a break, HTs and HTLs do not make diagnoses or treat patients. I > am a registered HT and a Florida licensed HTL with 19 years experience, > I've done it all in the lab. I believe the certification and licensure of > techs is a scam to bleed more money from people. Honestly, you can train a > monkey to do our job. And I don't want to hear from everyone saying it's an > art form, we are just as much needed as pathologists, blah, blah, > blah....... I work where they are hiring people from a masters degree > program for histology with certification, THEY KNOW NOTHING. Experience it > where it's at, whether certified or not, get off your high horse. > > > > > > > > > > > > > > > > > > > > > > > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From chapcl <@t> yahoo.com Thu May 24 18:02:45 2012 From: chapcl <@t> yahoo.com (William Chappell) Date: Thu May 24 18:02:49 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism. There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive. Neither are better than the other, both are needed. I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group. I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home. they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide. Jay refers to these people as no better than trained monkeys. That is a horrible insult with a small (very small) grain of truth. One day those histologists will be replaced by a mechanical/robotic process. The march of progress is unstoppable. The career histologist has a much longer life span however. We analyze and troubleshoot problems. We understand or endeavor to learn the organic chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and special stains. And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: > I'm sorry - I cannot let this rest. The comment: "we are just as much > needed as pathologists, blah, blah, > blah......." is so upsetting I cannot sit back and listen to that without > saying something! > > Everyone, regardless of their lot in life, is a very worthwhile part of the > whole. Let me ask you a question, since you highly undervalue humans that > are not MD's - let's say that you are a patient at Hospital X, and you go > in to have your toenail removed. Who plays a more important role in your > survival - the Podiatrist or the hospital janitor? I would argue that the > janitor is more crucial in this instance, for if he/she fails to clean up > the MRSA from the last patient you could conceivably die. The doctor solved > your fungal problem, but the janitor prevented you from getting a > potentially life-threatening infection. Think before you speak like that - > everyone involved in your care is critical - and, yes, sometimes the doctor > is not the most important person when it comes to keeping you alive and > well! > > > > > > On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren wrote: > >> Scott Lyons slnj07@yahoo.com >> >> Give me a break, HTs and HTLs do not make diagnoses or treat patients. I >> am a registered HT and a Florida licensed HTL with 19 years experience, >> I've done it all in the lab. I believe the certification and licensure of >> techs is a scam to bleed more money from people. Honestly, you can train a >> monkey to do our job. And I don't want to hear from everyone saying it's an >> art form, we are just as much needed as pathologists, blah, blah, >> blah....... I work where they are hiring people from a masters degree >> program for histology with certification, THEY KNOW NOTHING. Experience it >> where it's at, whether certified or not, get off your high horse. >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu May 24 18:25:59 2012 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu May 24 18:26:04 2012 Subject: [Histonet] Unregistered techs References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: <90354A475B420441B2A0396E5008D49692C0C0@copc-sbs.COPC.local> Holy buckets, that's a shot below the belt! Must say, I'm quite surprised to see a comment like that from someone with 19 years experience. By the by, I understand the registered HT thing, what is a "Florida licensed HTL"? Is that something new? But I digress. I can see your point about the scam/bleed money thing, but that's another discussion. You think monkeys can be trained to do histology...well, you're entitled to your opinion. However, the validity of an opinion depends on its basis. In my opinion monkeys cannot be trained to do our job. I'm quite certain that everything I did in the lab today (before returning to my office, reading this post and writing my response) would be challenging for a lot of folks...pathologists included...let alone a smart monkey. I'm a bit confused seeing the name Scott Lyons in the post below, so I don't want to direct my response to the wrong person. If this is indeed you, Jay, I've read many of your posts in the past. In consideration of that, I'm thinking maybe you're exaggerating to make a point, maybe having/had a bad day or both. I agree there is no substitute for experience. And I agree that many people with advanced degrees can be all thumbs in a lab, or maybe have a hard time transitioning book learning into hands on action. Come to think of it monkeys are pretty dexterous...so maybe we're taking this all wrong. I'm not responding to "light someone up" or get into a war or words with. I'll just say that I hold those of us doing this work in high regard, monkey or not. And that includes you too Jay...I've not met you personally, but honestly you're no monkey. Regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Thursday, May 24, 2012 2:02 PM To: Kim Tournear Cc: histonet Subject: Re: [Histonet] Unregistered techs Scott Lyons slnj07@yahoo.com Give me a break, HTs and HTLs do not make diagnoses or treat patients. I am a registered HT and a Florida licensed HTL with 19 years experience, I've done it all in the lab. I believe the certification and licensure of techs is a scam to bleed more money from people. Honestly, you can train a monkey to do our job. And I don't want to hear from everyone saying it's an art form, we are just as much needed as pathologists, blah, blah, blah....... I work where they are hiring people from a masters degree program for histology with certification, THEY KNOW NOTHING. Experience it where it's at, whether certified or not, get off your high horse. > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu May 24 18:27:55 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 24 18:28:18 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: <004e01cd3a04$d8ec7df0$8ac579d0$@rr.com> Let me add more fuel to this fire. Will makes strong points. I have been in this field over 35 years ( has it been that long) Any way. I have worked in and have managed many labs. I have had registered and unregistered techs. Some good, some not so good, some I told that their talents would be better served in another career field. Some people in histology come for the pay, others for a career. However, I have seen some clinicians, nurses and other healthcare providers do the same. I went to a neurologist once (emphasis on once). I was trying to explain to him my lengthy previous medical history, which has been plagued by heart problems for years. He was not interested in that. He wanted to get me in and get me out within the 15 minute time limit. My point is this: I don't care what job you do, there are going to be people who look at it as a job, others look at as a career. My youngest sister had some cognitive issues. She worked at minimum wage jobs all her life. One job was at a laundry mat that had several large accounts. I met her for lunch one day before I joined the Air Force. I watched her fold sheets so tight that they looked like you would cut your fingers on them if you ran them across the creases. I asked her why she took so much care in folding the sheets. She looked at me and said "Joey, I do it because anything you do, you have to do it good. If you ain't gonna do it good, don't do it at all". I still carry that notion and I hope I have passed that idea onto my children. When I was in Basic Training, making my bunk, I would always think of that day with my sister. Consider yourself lucky if you work with more people who think Histology as a career rather than just a job. I always do. I'll get off my soap box now and return you to regular programing. Joe Nocito -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Chappell Sent: Thursday, May 24, 2012 6:03 PM To: Davide Costanzo Cc: histonet Subject: Re: [Histonet] Unregistered techs I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism. There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive. Neither are better than the other, both are needed. I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group. I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home. they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide. Jay refers to these people as no better than trained monkeys. That is a horrible insult with a small (very small) grain of truth. One day those histologists will be replaced by a mechanical/robotic process. The march of progress is unstoppable. The career histologist has a much longer life span however. We analyze and troubleshoot problems. We understand or endeavor to learn the organic chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and special stains. And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: > I'm sorry - I cannot let this rest. The comment: "we are just as much > needed as pathologists, blah, blah, > blah......." is so upsetting I cannot sit back and listen to that without > saying something! > > Everyone, regardless of their lot in life, is a very worthwhile part of the > whole. Let me ask you a question, since you highly undervalue humans that > are not MD's - let's say that you are a patient at Hospital X, and you go > in to have your toenail removed. Who plays a more important role in your > survival - the Podiatrist or the hospital janitor? I would argue that the > janitor is more crucial in this instance, for if he/she fails to clean up > the MRSA from the last patient you could conceivably die. The doctor solved > your fungal problem, but the janitor prevented you from getting a > potentially life-threatening infection. Think before you speak like that - > everyone involved in your care is critical - and, yes, sometimes the doctor > is not the most important person when it comes to keeping you alive and > well! > > > > > > On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren wrote: > >> Scott Lyons slnj07@yahoo.com >> >> Give me a break, HTs and HTLs do not make diagnoses or treat patients. I >> am a registered HT and a Florida licensed HTL with 19 years experience, >> I've done it all in the lab. I believe the certification and licensure of >> techs is a scam to bleed more money from people. Honestly, you can train a >> monkey to do our job. And I don't want to hear from everyone saying it's an >> art form, we are just as much needed as pathologists, blah, blah, >> blah....... I work where they are hiring people from a masters degree >> program for histology with certification, THEY KNOW NOTHING. Experience it >> where it's at, whether certified or not, get off your high horse. >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu May 24 20:08:58 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu May 24 20:09:10 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: <8CF082A18F2D598-1960-535B9@webmail-d033.sysops.aol.com> I've personally tried to train monkeys to do this - they suck at it. I've trained a lot of histotechs, and learned early on that not just any Joe Schmo can do this job (my apologies to any real Joe Schmo's out there). There is a certain skill level and intelligence needed to perform good microtomy, optimize and antibody, or troubleshoot a special stain. I've been in labs where people were just told 'this is a block, put it in the holder on that machine, crank the handle as fast as you can, and pick up what comes off". LITERALLY. This is a skill, and it requires talent. To be good at it requires intelligence and good training. To be great at it requires desire. You're really lucky if you love your job, and I do love this work. I can clicker train monkeys and dogs, but not histotechs. Jackie O' -----Original Message----- From: William Chappell To: Davide Costanzo Cc: histonet Sent: Thu, May 24, 2012 6:02 pm Subject: Re: [Histonet] Unregistered techs I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism. There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive. Neither are better than the other, both are needed. I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group. I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home. they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide. Jay refers to these people as no better than trained monkeys. That is a horrible insult with a small (very small) grain of truth. One day those histologists will be replaced by a mechanical/robotic process. The march of progress is unstoppable. The career histologist has a much longer life span however. We analyze and troubleshoot problems. We understand or endeavor to learn the organic chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and special stains. And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: > I'm sorry - I cannot let this rest. The comment: "we are just as much > needed as pathologists, blah, blah, > blah......." is so upsetting I cannot sit back and listen to that without > saying something! > > Everyone, regardless of their lot in life, is a very worthwhile part of the > whole. Let me ask you a question, since you highly undervalue humans that > are not MD's - let's say that you are a patient at Hospital X, and you go > in to have your toenail removed. Who plays a more important role in your > survival - the Podiatrist or the hospital janitor? I would argue that the > janitor is more crucial in this instance, for if he/she fails to clean up > the MRSA from the last patient you could conceivably die. The doctor solved > your fungal problem, but the janitor prevented you from getting a > potentially life-threatening infection. Think before you speak like that - > everyone involved in your care is critical - and, yes, sometimes the doctor > is not the most important person when it comes to keeping you alive and > well! > > > > > > On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren wrote: > >> Scott Lyons slnj07@yahoo.com >> >> Give me a break, HTs and HTLs do not make diagnoses or treat patients. I >> am a registered HT and a Florida licensed HTL with 19 years experience, >> I've done it all in the lab. I believe the certification and licensure of >> techs is a scam to bleed more money from people. Honestly, you can train a >> monkey to do our job. And I don't want to hear from everyone saying it's an >> art form, we are just as much needed as pathologists, blah, blah, >> blah....... I work where they are hiring people from a masters degree >> program for histology with certification, THEY KNOW NOTHING. Experience it >> where it's at, whether certified or not, get off your high horse. >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gina.Rodriguez <@t> leica-microsystems.com Thu May 24 22:03:34 2012 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Thu May 24 22:03:40 2012 Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 05/29/2012) Message-ID: I am out of the office until 05/29/2012. I will respond to your message when I return. If you need immediate assistance please contact 800-248-0123 or Tech.support@leica-microsystems.com Note: This is an automated response to your message "Histonet Digest, Vol 102, Issue 32" sent on 5/24/2012 8:08:27 PM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From one_angel_secret <@t> yahoo.com Fri May 25 06:23:19 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri May 25 06:23:24 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> Message-ID: <1337944999.87501.YahooMailNeo@web112306.mail.gq1.yahoo.com> LOL, I was waiting for this thread to turn into a fist fight. I knew it would. ? A Fl Licensed HTL is someone who oviously had to take more test than a ASCP certified tech if they got it from 19 years ago. How do I know, I'm one of those who had to take all those test way back then. Florida is pretty stringent who can do what with what. Not as much as they used to be, because now the only route is through ASCP. You will not work in a hospital as a histologist without a Fl License. Maybe in a exclusive provider private office, but that is the only exception. ? As far as Monkeys. Whew! I think that's whats wrong with our profession, one thing anyway. Too many people veiw it that way. ? Personally I wish the Histo programs would go back to teaching on campus with using the MLT course comparison. Not to bash anyone who is either involved or has been involved with the internet programs that have popped up everywhere, but I'm not seeing the same level of technical skill or knowledge come out of these programs. I'm sure there are many super students who do get it, but many times they are pushed through on thier short rotations and used as gophers etc and dont have the skills even sometimes to cut a block. And forget about knowing what a good control for a GMS is. ? I'd like to see our profession go back to min AS degrees in Histology. The certification only have hurt us and the pay scale is changing I feel because of it. ? Also, if you really want to promote our field and improve it, be supportive of your state going to a license for our practice. ASCP supports states requiring license. ? Thats my 2cents for the day. Happy Friday! :) ? Kim D ________________________________ From: William Chappell To: Davide Costanzo Cc: histonet Sent: Thursday, May 24, 2012 7:02 PM Subject: Re: [Histonet] Unregistered techs I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism.? There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive.? Neither are better than the other, both are needed.? I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group.? I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home.? they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide.? Jay refers to these people as no better than trained monkeys.? That is a horrible insult with a small (very small) grain of truth.? One day those histologists will be replaced by a mechanical/robotic process.? The march of progress is unstoppable. The career histologist has a much longer life span however.? We analyze and troubleshoot problems.? We understand or endeavor to learn the organic chemistry of stains.? We know EXACTLY how a Rabbit Monoclonal antibody is made.? We know more about the practice of histology than ANY pathologist.? We invent and develop antibodies and special stains.? And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended.? We don't do this simply because it is a good paycheck.? We are histologists because we are professionals who choose this career.? You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: > I'm sorry - I cannot let this rest. The comment: "we are just as much > needed as pathologists, blah, blah, > blah......." is so upsetting I cannot sit back and listen to that without > saying something! > > Everyone, regardless of their lot in life, is a very worthwhile part of the > whole. Let me ask you a question, since you highly undervalue humans that > are not MD's - let's say that you are a patient at Hospital X, and you go > in to have your toenail removed. Who plays a more important role in your > survival - the Podiatrist or the hospital janitor? I would argue that the > janitor is more crucial in this instance, for if he/she fails to clean up > the MRSA from the last patient you could conceivably die. The doctor solved > your fungal problem, but the janitor prevented you from getting a > potentially life-threatening infection. Think before you speak like that - > everyone involved in your care is critical - and, yes, sometimes the doctor > is not the most important person when it comes to keeping you alive and > well! > > > > > > On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren wrote: > >> Scott Lyons slnj07@yahoo.com >> >> Give me a break, HTs and HTLs do not make diagnoses or treat patients. I >> am a registered HT and a Florida licensed HTL with 19 years experience, >> I've done it all in the lab. I believe the certification and licensure of >> techs is a scam to bleed more money from people. Honestly, you can train a >> monkey to do our job. And I don't want to hear from everyone saying it's an >> art form, we are just as much needed as pathologists, blah, blah, >> blah....... I work where they are hiring people from a masters degree >> program for histology with certification, THEY KNOW NOTHING. Experience it >> where it's at, whether certified or not, get off your high horse. >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From keeping.janet <@t> gmail.com Fri May 25 06:37:38 2012 From: keeping.janet <@t> gmail.com (Janet Keeping) Date: Fri May 25 06:37:45 2012 Subject: [Histonet] certification of histotechnologists Message-ID: Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. From ree3 <@t> leicester.ac.uk Fri May 25 07:35:39 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri May 25 07:36:01 2012 Subject: [Histonet] certification of histotechnologists In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A216@EXC-MBX3.cfs.le.ac.uk> Same in UK, I have never understood why the separation in the Land of the Free. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: 25 May 2012 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri May 25 07:45:08 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri May 25 07:45:19 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <8CF082A18F2D598-1960-535B9@webmail-d033.sysops.aol.com> References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> <8CF082A18F2D598-1960-535B9@webmail-d033.sysops.aol.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E253330@evcspmbx3.ads.northwestern.edu> Than you Jackie. That was the point I was trying to make. I like my job and I hate it when researchers think "anyone can do histology", yet even though the MD has to have us tell him or his Post-docs what they need or do not need. Bernce Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, May 24, 2012 8:09 PM To: chapcl@yahoo.com; pathlocums@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered techs I've personally tried to train monkeys to do this - they suck at it. I've trained a lot of histotechs, and learned early on that not just any Joe Schmo can do this job (my apologies to any real Joe Schmo's out there). There is a certain skill level and intelligence needed to perform good microtomy, optimize and antibody, or troubleshoot a special stain. I've been in labs where people were just told 'this is a block, put it in the holder on that machine, crank the handle as fast as you can, and pick up what comes off". LITERALLY. This is a skill, and it requires talent. To be good at it requires intelligence and good training. To be great at it requires desire. You're really lucky if you love your job, and I do love this work. I can clicker train monkeys and dogs, but not histotechs. Jackie O' -----Original Message----- From: William Chappell To: Davide Costanzo Cc: histonet Sent: Thu, May 24, 2012 6:02 pm Subject: Re: [Histonet] Unregistered techs I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism. There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive. Neither are better than the other, both are needed. I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group. I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home. they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide. Jay refers to these people as no better than trained monkeys. That is a horrible insult with a small (very small) grain of truth. One day those histologists will be replaced by a mechanical/robotic process. The march of progress is unstoppable. The career histologist has a much longer life span however. We analyze and troubleshoot problems. We understand or endeavor to learn the organic chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and special stains. And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: > I'm sorry - I cannot let this rest. The comment: "we are just as much > needed as pathologists, blah, blah, blah......." is so upsetting I > cannot sit back and listen to that without saying something! > > Everyone, regardless of their lot in life, is a very worthwhile part > of the whole. Let me ask you a question, since you highly undervalue > humans that are not MD's - let's say that you are a patient at > Hospital X, and you go in to have your toenail removed. Who plays a > more important role in your survival - the Podiatrist or the hospital > janitor? I would argue that the janitor is more crucial in this > instance, for if he/she fails to clean up the MRSA from the last > patient you could conceivably die. The doctor solved your fungal > problem, but the janitor prevented you from getting a potentially > life-threatening infection. Think before you speak like that - > everyone involved in your care is critical - and, yes, sometimes the > doctor is not the most important person when it comes to keeping you alive and well! > > > > > > On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren wrote: > >> Scott Lyons slnj07@yahoo.com >> >> Give me a break, HTs and HTLs do not make diagnoses or treat >> patients. I am a registered HT and a Florida licensed HTL with 19 >> years experience, I've done it all in the lab. I believe the >> certification and licensure of techs is a scam to bleed more money >> from people. Honestly, you can train a monkey to do our job. And I >> don't want to hear from everyone saying it's an art form, we are just >> as much needed as pathologists, blah, blah, blah....... I work where >> they are hiring people from a masters degree program for histology >> with certification, THEY KNOW NOTHING. Experience it where it's at, whether certified or not, get off your high horse. >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Fri May 25 08:25:38 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri May 25 08:25:17 2012 Subject: [Histonet] RE: Not requiring HT Certification) Message-ID: Like everyone else, I was going to keep quiet, but I can't. I am sensitive to those downgrading us who took the OJT route. I did the OJT route, had a BS in Biology, tried for Veterinary School, but that wasn't so. What do I do with all of this Science? As part of my Pre-Med curricula had to take histology, loved it. Had no idea that it was a paying field and such. Had I known, I would have applied to a school as a backup. It took me a while, but when I was hired as a tech, I did not excel quickly (Cheryl remember my mistakes), but I learned and had patient coworkers. Move on down the line several years and another coworker questioned my knowledge of the chemistry behind a stain, and all of a sudden all of my organic and biochem can running out of my mouth. I didn't realize that I really knew all of that. I had always felt a little disadvantaged because I did not get formal training through a school, just studied and passed the HT with the help of my coworkers (shout out to LSU VetPath). There is nothing wrong with OJT for Biology majors. They should have the basic background to understand the chemistries and processes behind why we do what we do. With the modernization of technologies and procedures in the histo lab some formal education is needed. There are many ways to receive this education, it can be online, or in person. It all depends on the learner. To overcome the stigma we should continue with some of the things that are now in place to stabilize the training of our successors (yes we all are going to have to retire one day). A continued push for formal training, promotion of the field, professionalism by our colleagues, and respect from the customers (pathologists, patients, and gen lab personnel). In order to facilitate change for respect, we must first present a unified front. I know good techs with no certification, I know bad techs with certification. We all do. Last year I celebrated my 20th year in histo and never thought I would be where I am. I never wanted to do research, and didn't like management (don't like telling grown folks what to do), but I love teaching. It helps me to learn the theory behind what I do and apply it better. There are so many people who can do histo whether it is routine, special procedures, or management. We shouldn't look down on those who took the OJT route, sometimes they just don't know about a formal program (like me). You never know where the next great manager, director or tech is coming from, so don't count them out. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Thu, 24 May 2012 17:20:41 +0000 From: joelle weaver Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Jon There is a route with associates and training I believe. Of course I can't speak for the BOC, and I am sure that you want to help your employees as much as you can. I do see your point about the similarities in tasks. My thought would be that the exam eligibility states that they have to have recent experience in fixation, embedding, microtomy, and staining (histology) and the associated theory knowledge. EM is on the exam study topics, but also with the theory/experience for all those routine histological techniques, is how I read it. Take a look at the exam outlines, that should give you an idea of the scope. Ascp.org "get certified". As I have been told, they want to cover the widest possible scope of roles histologists can perform, which could include EM, but not only that. If they don't have exposure to regular histology I think that it might be hard for to feel prepared for the regular HT or HTL exams. That's just my opinion, based on what I have observed and also the pass rates ( ~ 65%), for people even with training/experience- there could be an exceptional person out there. I can understand not wanting to get buried in doing a whole HT curricula ( believe me, I do). How about the option of having cross training in a histology lab? Do you have routine histology on site or a nearby lab? The best advice I can give is to go to the website and carefully read the requirments to see how your employees might fit in. If you want to provide the theory without having to do the curricula, there are on line programs out there which can supplement OJT and a supportive mentor and organization. I have seen this work successfully with motivated people with the ability to have hands on practice alongside. I suggest the NSH site which lists the accredited programs or the NAACLS site which has a search for programs, if that would help. As far as employability, my opinion is that it would certainly open up options for your employees to also have skills in routine histology make them more valuable to your organization, and I would think certification would be even more helpful to them as far as options. Joelle Weaver MAOM, HTL (ASCP) QIHC > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > From: jkrupp@deltacollege.edu > Date: Thu, 24 May 2012 09:53:21 -0700 > CC: histonet@lists.utsouthwestern.edu > To: joelleweaver@hotmail.com > > > On May 24, 2012, at 9:25 AM, joelle weaver wrote: > > > > > I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. > > OK, so I have a question. > > We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. > > So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? > > Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > ------------------------------ Message: 2 Date: Thu, 24 May 2012 17:28:10 +0000 From: joelle weaver Subject: RE: [Histonet] RE: Not requiring HT Certification To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes I was referring to the HS and OJT training route. There is confusion about the degree + OJT and HS + OJT. Yes, that was my understanding also of the drivers, I just could not recall where I read/heard that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 24 May 2012 09:56:25 -0700 > Subject: [Histonet] RE: Not requiring HT Certification > > " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." > > Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. > > If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. > > > Tim Morken > > ******************************** From one_angel_secret <@t> yahoo.com Fri May 25 09:03:28 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri May 25 09:03:42 2012 Subject: [Histonet] RE: Not requiring HT Certification) In-Reply-To: References: Message-ID: <53D6ADCF-AF81-45B4-B416-6E2F7D856C7B@yahoo.com> I personally agree with your route. What I am most concerned with is so many want a quick route these days and don't want to put in the hard work to learn the theory. They just want to pass the test, get a job and make a big paycheck and expect the lab that hires them to actually teach them. I enjoy teaching myself to a degree but times are changing and we are expected to do more with less. We need graduating techs to be hit the ground ready day one. If we don't start to expect this you can count on with all the cut backs in health care that our profession will continue to be viewed as just a bunch of monkeys and therefore will be payed as a bunch of monkeys. And personally being refered to as a monkey ticks me off. I'm not a monkey. I'm a frog lol Sent from my iPhone On May 25, 2012, at 9:25 AM, "Mayer,Toysha N" wrote: > > > Like everyone else, I was going to keep quiet, but I can't. > I am sensitive to those downgrading us who took the OJT route. > I did the OJT route, had a BS in Biology, tried for Veterinary School, but that wasn't so. What do I do with all of this Science? As part of my Pre-Med curricula had to take histology, loved it. Had no idea that it was a paying field and such. Had I known, I would have applied to a school as a backup. It took me a while, but when I was hired as a tech, I did not excel quickly (Cheryl remember my mistakes), but I learned and had patient coworkers. > Move on down the line several years and another coworker questioned my knowledge of the chemistry behind a stain, and all of a sudden all of my organic and biochem can running out of my mouth. I didn't realize that I really knew all of that. I had always felt a little disadvantaged because I did not get formal training through a school, just studied and passed the HT with the help of my coworkers (shout out to LSU VetPath). > There is nothing wrong with OJT for Biology majors. They should have the basic background to understand the chemistries and processes behind why we do what we do. With the modernization of technologies and procedures in the histo lab some formal education is needed. There are many ways to receive this education, it can be online, or in person. It all depends on the learner. > To overcome the stigma we should continue with some of the things that are now in place to stabilize the training of our successors (yes we all are going to have to retire one day). A continued push for formal training, promotion of the field, professionalism by our colleagues, and respect from the customers (pathologists, patients, and gen lab personnel). > In order to facilitate change for respect, we must first present a unified front. > I know good techs with no certification, I know bad techs with certification. We all do. > Last year I celebrated my 20th year in histo and never thought I would be where I am. I never wanted to do research, and didn't like management (don't like telling grown folks what to do), but I love teaching. It helps me to learn the theory behind what I do and apply it better. There are so many people who can do histo whether it is routine, special procedures, or management. > We shouldn't look down on those who took the OJT route, sometimes they just don't know about a formal program (like me). You never know where the next great manager, director or tech is coming from, so don't count them out. > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 24 May 2012 17:20:41 +0000 > From: joelle weaver > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Jon There is a route with associates and training I believe. > Of course I can't speak for the BOC, and I am sure that you want to help your employees as much as you can. I do see your point about the similarities in tasks. My thought would be that the exam eligibility states that they have to have recent experience in fixation, embedding, microtomy, and staining (histology) and the associated theory knowledge. EM is on the exam study topics, but also with the theory/experience for all those routine histological techniques, is how I read it. Take a look at the exam outlines, that should give you an idea of the scope. Ascp.org "get certified". As I have been told, they want to cover the widest possible scope of roles histologists can perform, which could include EM, but not only that. If they don't have exposure to regular histology I think that it might be hard for to feel prepared for the regular HT or HTL exams. That's just my opinion, based on what I have observed and also the pass rates ( ~ 65%), for people even with training/experience- there could be an exceptional person out there. I can understand not wanting to get buried in doing a whole HT curricula ( believe me, I do). How about the option of having cross training in a histology lab? Do you have routine histology on site or a nearby lab? The best advice I can give is to go to the website and carefully read the requirments to see how your employees might fit in. If you want to provide the theory without having to do the curricula, there are on line programs out there which can supplement OJT and a supportive mentor and organization. I have seen this work successfully with motivated people with the ability to have hands on practice alongside. I suggest the NSH site which lists the accredited programs or the NAACLS site which has a search for programs, if that would help. As far as employability, my opinion is that it would certainly open up options for your employees to also have skills in routine histology make them more valuable to your organization, and I would think certification would be even more helpful to them as far as options. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) >> From: jkrupp@deltacollege.edu >> Date: Thu, 24 May 2012 09:53:21 -0700 >> CC: histonet@lists.utsouthwestern.edu >> To: joelleweaver@hotmail.com >> >> >> On May 24, 2012, at 9:25 AM, joelle weaver wrote: >> >>> >>> I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. >> >> OK, so I have a question. >> >> We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. >> >> So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? >> >> Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. >> >> Jon >> >> Jonathan Krupp >> Delta College >> 5151 Pacific Ave. >> Box 212 >> Stockton, CA 95207 >> 209-954-5284 >> jkrupp@deltacollege.edu >> >> Find us on Facebook @ >> Electron Microscopy at SJ Delta College >> >> >> >> >> >> > > > ------------------------------ > > Message: 2 > Date: Thu, 24 May 2012 17:28:10 +0000 > From: joelle weaver > Subject: RE: [Histonet] RE: Not requiring HT Certification > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Yes I was referring to the HS and OJT training route. There is confusion about the degree + OJT and HS + OJT. Yes, that was my understanding also of the drivers, I just could not recall where I read/heard that. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: Timothy.Morken@ucsfmedctr.org >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 24 May 2012 09:56:25 -0700 >> Subject: [Histonet] RE: Not requiring HT Certification >> >> " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." >> >> Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. >> >> If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. >> >> >> Tim Morken >> >> > ******************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Fri May 25 09:16:52 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri May 25 09:17:08 2012 Subject: [Histonet] RE: Not requiring HT Certification) In-Reply-To: References: Message-ID: I studies at the famed AFIP while in the military and have two degrees and never thought I would stay in histology. 22 years later I am still in the field. I don't think OJT is a bad route it has often generated some of our best techs but I am a firm believer that new techs need to go through a formal program or have a B.S. degree. I feel this way because most facilities require a degree to move up in positions of authority. I have a wonderful tech that went through a formal associates histology program but does not have a B.S. degree, my facility will not allow me to promote her to a assistant manager position. So if you are a young tech out there and you have dreams of moving up into a leadership position, get a B.S. degree in something. That is why med techs are held in higher regards and why most assistant director and director positions come from the clinical lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Friday, May 25, 2012 8:26 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Not requiring HT Certification) Like everyone else, I was going to keep quiet, but I can't. I am sensitive to those downgrading us who took the OJT route. I did the OJT route, had a BS in Biology, tried for Veterinary School, but that wasn't so. What do I do with all of this Science? As part of my Pre-Med curricula had to take histology, loved it. Had no idea that it was a paying field and such. Had I known, I would have applied to a school as a backup. It took me a while, but when I was hired as a tech, I did not excel quickly (Cheryl remember my mistakes), but I learned and had patient coworkers. Move on down the line several years and another coworker questioned my knowledge of the chemistry behind a stain, and all of a sudden all of my organic and biochem can running out of my mouth. I didn't realize that I really knew all of that. I had always felt a little disadvantaged because I did not get formal training through a school, just studied and passed the HT with the help of my coworkers (shout out to LSU VetPath). There is nothing wrong with OJT for Biology majors. They should have the basic background to understand the chemistries and processes behind why we do what we do. With the modernization of technologies and procedures in the histo lab some formal education is needed. There are many ways to receive this education, it can be online, or in person. It all depends on the learner. To overcome the stigma we should continue with some of the things that are now in place to stabilize the training of our successors (yes we all are going to have to retire one day). A continued push for formal training, promotion of the field, professionalism by our colleagues, and respect from the customers (pathologists, patients, and gen lab personnel). In order to facilitate change for respect, we must first present a unified front. I know good techs with no certification, I know bad techs with certification. We all do. Last year I celebrated my 20th year in histo and never thought I would be where I am. I never wanted to do research, and didn't like management (don't like telling grown folks what to do), but I love teaching. It helps me to learn the theory behind what I do and apply it better. There are so many people who can do histo whether it is routine, special procedures, or management. We shouldn't look down on those who took the OJT route, sometimes they just don't know about a formal program (like me). You never know where the next great manager, director or tech is coming from, so don't count them out. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Thu, 24 May 2012 17:20:41 +0000 From: joelle weaver Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Jon There is a route with associates and training I believe. Of course I can't speak for the BOC, and I am sure that you want to help your employees as much as you can. I do see your point about the similarities in tasks. My thought would be that the exam eligibility states that they have to have recent experience in fixation, embedding, microtomy, and staining (histology) and the associated theory knowledge. EM is on the exam study topics, but also with the theory/experience for all those routine histological techniques, is how I read it. Take a look at the exam outlines, that should give you an idea of the scope. Ascp.org "get certified". As I have been told, they want to cover the widest possible scope of roles histologists can perform, which could include EM, but not only that. If they don't have exposure to regular histology I think that it might be hard for to feel prepared for the regular HT or HTL exams. That's just my opinion, based on what I have observed and also the pass rates ( ~ 65%), for people even with training/experience- there could be an exceptional person out there. I can understand not wanting to get buried in doing a whole HT curricula ( believe me, I do). How about the option of having cross training in a histology lab? Do you have routine histology on site or a nearby lab? The best advice I can give is to go to the website and carefully read the requirments to see how your employees might fit in. If you want to provide the theory without having to do the curricula, there are on line programs out there which can supplement OJT and a supportive mentor and organization. I have seen this work successfully with motivated people with the ability to have hands on practice alongside. I suggest the NSH site which lists the accredited programs or the NAACLS site which has a search for programs, if that would help. As far as employability, my opinion is that it would certainly open up options for your employees to also have skills in routine histology make them more valuable to your organization, and I would think certification would be even more helpful to them as far as options. Joelle Weaver MAOM, HTL (ASCP) QIHC > Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) > From: jkrupp@deltacollege.edu > Date: Thu, 24 May 2012 09:53:21 -0700 > CC: histonet@lists.utsouthwestern.edu > To: joelleweaver@hotmail.com > > > On May 24, 2012, at 9:25 AM, joelle weaver wrote: > > > > > I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. > > OK, so I have a question. > > We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. > > So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? > > Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > ------------------------------ Message: 2 Date: Thu, 24 May 2012 17:28:10 +0000 From: joelle weaver Subject: RE: [Histonet] RE: Not requiring HT Certification To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes I was referring to the HS and OJT training route. There is confusion about the degree + OJT and HS + OJT. Yes, that was my understanding also of the drivers, I just could not recall where I read/heard that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 24 May 2012 09:56:25 -0700 > Subject: [Histonet] RE: Not requiring HT Certification > > " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." > > Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. > > If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. > > > Tim Morken > > ******************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From bboyce <@t> NEMOURS.ORG Fri May 25 09:44:04 2012 From: bboyce <@t> NEMOURS.ORG (Boyce, Bobbie) Date: Fri May 25 09:44:14 2012 Subject: [Histonet] Embed-It Message-ID: Hello all, We are getting ready for a project that uses Embed-It(tm). We are embedding decalcified bones in the plastic to obtain 0.5um sections. Has anyone had any experience with this product? It looks like a fairly simple kit. Much different than the Ostoebed(tm). Any helpful hits would be great. Bobbie Boyce Histology Specialist III duPont Hospital for Children Wilmington, DE 302-651-6771 (Lab) 302-651-5010 (Fax) From MSHERWOOD <@t> PARTNERS.ORG Fri May 25 10:18:49 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri May 25 10:22:37 2012 Subject: [Histonet] RE: Embed-It In-Reply-To: References: Message-ID: <090FA56107A969459F3941DDD5585C3A1175DD2F@PHSX10MB10.partners.org> I've never heard of it. Who sells this product? I tried googling, but could not find the site. Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyce, Bobbie Sent: Friday, May 25, 2012 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embed-It Hello all, We are getting ready for a project that uses Embed-It(tm). We are embedding decalcified bones in the plastic to obtain 0.5um sections. Has anyone had any experience with this product? It looks like a fairly simple kit. Much different than the Ostoebed(tm). Any helpful hits would be great. Bobbie Boyce Histology Specialist III duPont Hospital for Children Wilmington, DE 302-651-6771 (Lab) 302-651-5010 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Timothy.Morken <@t> ucsfmedctr.org Fri May 25 10:55:58 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 25 10:55:52 2012 Subject: [Histonet] certification of histotechnologists In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF8B5187DE7@MCINFRWEM003.ucsfmedicalcenter.org> Janet brings up an interesting point. The rest of the world (ie, besides US) has histo as part of the med tech program and then they specialize in their final year. I have worked with techs from many other countries and in general are far more knowledgeable than the majority of even certified techs in the US. The US med tech programs dropped histo decades ago. I'm not sure why. Pathology labs certainly benefitted financially because it allowed them to hire literally anybody to do the work. But even in the US the med tech schools are declining due to lack of enrollment. Probably due to automation in laboratories they just don't need as many people. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Friday, May 25, 2012 4:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri May 25 11:10:22 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri May 25 11:10:31 2012 Subject: [Histonet] certification of Histotechnologists Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32406666EF@Mail2Node2.ad.uams.edu> The programs were dropped as the pathologist could still hold the training etcetera to suit their needs and not worry about allowing histologist to become part of the professional/complex testing world. In the 1960s when this happened we did few of the tests we are asked to do now and really rarely looked at slides. Microscopes were rarely available to most of us to even review our work. The changes in the field from routine histology to IHC have not been accounted for by ASCP and so far NSH has not made the grade for getting the field recognized as a part of the professional/complex testing world. We now have QIHC however; it does not help those of us in the Histology side in any way. The rest of the world is very different in how they approach this field and why it is needed for patient care. I sometimes feel here we are only servants to the pathologist and what they want. The pay scale has not gotten much better while the automated field in med tech are being paid more and required to do less hands on actual testing the way we do in Histology. Pam Mar -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, May 25, 2012 10:56 AM To: Janet Keeping; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification of histotechnologists Janet brings up an interesting point. The rest of the world (ie, besides US) has histo as part of the med tech program and then they specialize in their final year. I have worked with techs from many other countries and in general are far more knowledgeable than the majority of even certified techs in the US. The US med tech programs dropped histo decades ago. I'm not sure why. Pathology labs certainly benefitted financially because it allowed them to hire literally anybody to do the work. But even in the US the med tech schools are declining due to lack of enrollment. Probably due to automation in laboratories they just don't need as many people. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Friday, May 25, 2012 4:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From nicole <@t> dlcjax.com Fri May 25 11:35:16 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Fri May 25 11:35:22 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <1337944999.87501.YahooMailNeo@web112306.mail.gq1.yahoo.com> References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> <1337944999.87501.YahooMailNeo@web112306.mail.gq1.yahoo.com> Message-ID: <2344.208.62.167.196.1337963716.squirrel@webmail.realpages.com> >LOL, I dont have much to say about this one. Like you Kim I have a fl HTL license and an HT ASCP. I have two classes left before I complete my BSH and will be able to sit for my ASCP HTL. I have 12yrs of experience but that is not reconginized in the eyes of licensure for ASCP. I do believe my skill level should determine my licensure status not the degree I hold. Because I promise every class im taking rt now means nothing towards my field. The word histology has not even be written in any of the books im studying. I think OJT techs are just as qualified as any person who completed an online programs, if not more so. I completed an online histology program and learned my skills from other people in the bizz with alot of experience who were willing to train me and be patient. Never one time did I question their, experience, licensure, or training. The OJTs carried Histology through the transisition of licensure and passed the skills and knowlegde on to us young bucks to carry the torch. That is all...lol Nicole Tatum HT ASCP (hahaha soon to be HTL ASCP) :)~~~~~~~ LOL, I was waiting for this thread to turn into a fist fight. I knew it > would. > ? > A Fl Licensed HTL is someone who oviously had to take more test than a > ASCP certified tech if they got it from 19 years ago. How do I know, I'm > one of those who had to take all those test way back then. Florida is > pretty stringent who can do what with what. Not as much as they used to > be, because now the only route is through ASCP. You will not work in a > hospital as a histologist without a Fl License. Maybe in a exclusive > provider private office, but that is the only exception. > ? > As far as Monkeys. Whew! I think that's whats wrong with our profession, > one thing anyway. Too many people veiw it that way. > ? > Personally I wish the Histo programs would go back to teaching on campus > with using the MLT course comparison. Not to bash anyone who is either > involved or has been involved with the internet programs that have popped > up everywhere, but I'm not seeing the same level of technical skill or > knowledge come out of these programs. I'm sure there are many super > students who do get it, but many times they are pushed through on thier > short rotations and used as gophers etc and dont have the skills even > sometimes to cut a block. And forget about knowing what a good control for > a GMS is. > ? > I'd like to see our profession go back to min AS degrees in Histology. The > certification only have hurt us and the pay scale is changing I feel > because of it. > ? > Also, if you really want to promote our field and improve it, be > supportive of your state going to a license for our practice. ASCP > supports states requiring license. > ? > Thats my 2cents for the day. Happy Friday! :) > ? > Kim D > > > ________________________________ > From: William Chappell > To: Davide Costanzo > Cc: histonet > Sent: Thursday, May 24, 2012 7:02 PM > Subject: Re: [Histonet] Unregistered techs > > I have respected Jay's input in the past, but I too must say something. > > Without realizing it, and by stating his opinion in a horribly crass way, > Jay has touched upon an important truism.? There are two types of > histologists, those that have a job that pays the bills, and those who > have a career in which they thrive.? Neither are better than the other, > both are needed.? I suspect, however, that the majority of Histonetters -- > especially avid contributors are in the latter group.? I know I am. > > Histotechs who approach histology as a job, go into work, embed, cut, > stain and go home.? they are excellent techs, but are just not committed > to expanding the field or doing more than is needed to provide the > pathologist with a perfect slide.? Jay refers to these people as no better > than trained monkeys.? That is a horrible insult with a small (very small) > grain of truth.? One day those histologists will be replaced by a > mechanical/robotic process.? The march of progress is unstoppable. > > The career histologist has a much longer life span however.? We analyze > and troubleshoot problems.? We understand or endeavor to learn the organic > chemistry of stains.? We know EXACTLY how a Rabbit Monoclonal antibody is > made.? We know more about the practice of histology than ANY pathologist.? > We invent and develop antibodies and special stains.? And we conceptualize > and perfect the instruments that will replace the first group in the > future. > > Jay, that is why so many are offended.? We don't do this simply because it > is a good paycheck.? We are histologists because we are professionals who > choose this career.? You may be going to a job cutting slides (which is > great and necessary), but we are enjoying our life. > > Will Chappell, HTL (ASCP), QIHC, MBA > and histologist by choice, not accident > > > On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: > >> I'm sorry - I cannot let this rest. The comment: "we are just as much >> needed as pathologists, blah, blah, >> blah......." is so upsetting I cannot sit back and listen to that >> without >> saying something! >> >> Everyone, regardless of their lot in life, is a very worthwhile part of >> the >> whole. Let me ask you a question, since you highly undervalue humans >> that >> are not MD's - let's say that you are a patient at Hospital X, and you >> go >> in to have your toenail removed. Who plays a more important role in your >> survival - the Podiatrist or the hospital janitor? I would argue that >> the >> janitor is more crucial in this instance, for if he/she fails to clean >> up >> the MRSA from the last patient you could conceivably die. The doctor >> solved >> your fungal problem, but the janitor prevented you from getting a >> potentially life-threatening infection. Think before you speak like that >> - >> everyone involved in your care is critical - and, yes, sometimes the >> doctor >> is not the most important person when it comes to keeping you alive and >> well! >> >> >> >> >> >> On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren >> wrote: >> >>> Scott Lyons slnj07@yahoo.com >>> >>> Give me a break, HTs and HTLs do not make diagnoses or treat patients. >>> I >>> am a registered HT and a Florida licensed HTL with 19 years experience, >>> I've done it all in the lab. I believe the certification and licensure >>> of >>> techs is a scam to bleed more money from people. Honestly, you can >>> train a >>> monkey to do our job. And I don't want to hear from everyone saying >>> it's an >>> art form, we are just as much needed as pathologists, blah, blah, >>> blah....... I work where they are hiring people from a masters degree >>> program for histology with certification, THEY KNOW NOTHING. Experience >>> it >>> where it's at, whether certified or not, get off your high horse. >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> *David Costanzo, MHS, PA (ASCP)* >> Project Manager >> *Blufrog Path Lab Solutions* >> 9401 Wilshire Blvd. Ste 650 >> Beverly Hills, CA 90212 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From thisisann <@t> aol.com Fri May 25 12:02:29 2012 From: thisisann <@t> aol.com (Ann Specian) Date: Fri May 25 12:02:46 2012 Subject: [Histonet] Embedding Message-ID: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> We are having a problem with floaters in our blocks which occur during embedding. We have multiple forceps which are placed in heated wells and each cassette is embedded with a new forcep. We also wipe with a gauze, but we are still getting floaters embedded in the cassette from time to time. Does anyone do anything else to prevent this? Thank you, Ann From kim.tournear <@t> yahoo.com Fri May 25 12:10:10 2012 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Fri May 25 12:10:20 2012 Subject: [Histonet] certification of Histotechnologists In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32406666EF@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA32406666EF@Mail2Node2.ad.uams.edu> Message-ID: <85DB63C2-7426-4ADD-A667-2CF87A3B7104@yahoo.com> Well said Pam. Sent from the iPhone of Kim Tournear. On May 25, 2012, at 11:10 AM, "Marcum, Pamela A" wrote: > The programs were dropped as the pathologist could still hold the training etcetera to suit their needs and not worry about allowing histologist to become part of the professional/complex testing world. In the 1960s when this happened we did few of the tests we are asked to do now and really rarely looked at slides. Microscopes were rarely available to most of us to even review our work. The changes in the field from routine histology to IHC have not been accounted for by ASCP and so far NSH has not made the grade for getting the field recognized as a part of the professional/complex testing world. We now have QIHC however; it does not help those of us in the Histology side in any way. > > The rest of the world is very different in how they approach this field and why it is needed for patient care. I sometimes feel here we are only servants to the pathologist and what they want. The pay scale has not gotten much better while the automated field in med tech are being paid more and required to do less hands on actual testing the way we do in Histology. > > Pam Mar > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Friday, May 25, 2012 10:56 AM > To: Janet Keeping; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] certification of histotechnologists > > Janet brings up an interesting point. The rest of the world (ie, besides US) has histo as part of the med tech program and then they specialize in their final year. I have worked with techs from many other countries and in general are far more knowledgeable than the majority of even certified techs in the US. The US med tech programs dropped histo decades ago. I'm not sure why. Pathology labs certainly benefitted financially because it allowed them to hire literally anybody to do the work. > > But even in the US the med tech schools are declining due to lack of enrollment. Probably due to automation in laboratories they just don't need as many people. > > Tim Morken > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping > Sent: Friday, May 25, 2012 4:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] certification of histotechnologists > > Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, > use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Fri May 25 12:12:18 2012 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Fri May 25 12:12:27 2012 Subject: [Histonet] Embedding In-Reply-To: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> References: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD00BDA1E0C@PPWEXD01a.childrens.sea.kids> We make sure to clean the wells also. Little flecks of tissue are almost always in there after embedding. Sally Norton Seattle Children's -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Friday, May 25, 2012 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding We are having a problem with floaters in our blocks which occur during embedding. We have multiple forceps which are placed in heated wells and each cassette is embedded with a new forcep. We also wipe with a gauze, but we are still getting floaters embedded in the cassette from time to time. Does anyone do anything else to prevent this? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From thisisann <@t> aol.com Fri May 25 12:14:53 2012 From: thisisann <@t> aol.com (Ann Specian) Date: Fri May 25 12:15:17 2012 Subject: [Histonet] Embedding In-Reply-To: <1357F84B33D39A46BA015A8EC6ABCBD00BDA1E0C@PPWEXD01a.childrens.sea.kids> References: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> <1357F84B33D39A46BA015A8EC6ABCBD00BDA1E0C@PPWEXD01a.childrens.sea.kids> Message-ID: <8CF08B10897E8C0-C74-95C4@webmail-m025.sysops.aol.com> we clean them at the start of our shift, but not during embedding. do you clean them during embedding too? -----Original Message----- From: Norton, Sally To: 'Ann Specian' ; histonet Sent: Fri, May 25, 2012 1:12 pm Subject: RE: [Histonet] Embedding We make sure to clean the wells also. Little flecks of tissue are almost always n there after embedding. Sally Norton eattle Children's -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Ann Specian ent: Friday, May 25, 2012 10:02 AM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Embedding e are having a problem with floaters in our blocks which occur during mbedding. We have multiple forceps which are placed in heated wells and each assette is embedded with a new forcep. We also wipe with a gauze, but we are till getting floaters embedded in the cassette from time to time. Does anyone do anything else to prevent this? hank you, Ann ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for he sole use of the intended recipient(s) and may contain confidential and rivileged information protected by law. Any unauthorized review, use, isclosure or distribution is prohibited. If you are not the intended ecipient, please contact the sender by reply e-mail and destroy all copies of he original message. From joelleweaver <@t> hotmail.com Fri May 25 12:21:49 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri May 25 12:21:57 2012 Subject: [Histonet] certification of histotechnologists In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B5187DE7@MCINFRWEM003.ucsfmedicalcenter.org> References: , <8D7C2D242DBD45498006B21122072BF8B5187DE7@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: These are interesting points. In my experience with the educational "wing" at least some ( non-histology educators) have about the same "monkey" assessment of what knowledge and skills are needed for histology as some others. They seemed to feel that an associates was unecessary, and also feel that any med tech, ( or just about anyone) can do histology, with or without training- so why do we need to have programs at all? Plus it's a money issue- based on enrollment. So on campus programs fell away and continue to do so. The alternative was to have the students train in an off site lab and attend class on campus. Finding those labs willing to take students for clinicals was challenging. Then came not even classes on campus, but on line. That seemed to provide something for some people. It is a better alternative than nothing, and some programs seem to be good, and can provide the theory. But based on my first hand experience, I came to the conclusion that for quite a few students, it just doesn't translate well. Before I get a "flurry" of angry responses, note that some people do quite well- it just depends on the individual and where their clinical site is. I just think that more people deserve to be set up to do well, and I think the education could be more unified and consistent. I am not even "blaming" educators, they do their best with what resources they are given- I just think that those resources are often not enough. I was lucky to have training in a program on campus, with good instructors, good clinicals, and then go on to work in a great lab with people who wanted me to succeed. I never entertained that this was a substitute for actual experience, but I did feel it gave me good fundamentals to get started, and I continue to learn everyday both on my own, and from others. Whenever I get sucked into this topic, it always gets construed that I am somehow insulting people who followed different paths- I am not! Experience is always valuable.There are good and bad examples in ALL professions, no matter the individual's education, training or experience. Education and experience are both valuable. They are just different ways of learning, and hopefully can work in synergy for each person. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: keeping.janet@gmail.com; histonet@lists.utsouthwestern.edu > Date: Fri, 25 May 2012 08:55:58 -0700 > Subject: RE: [Histonet] certification of histotechnologists > CC: > > Janet brings up an interesting point. The rest of the world (ie, besides US) has histo as part of the med tech program and then they specialize in their final year. I have worked with techs from many other countries and in general are far more knowledgeable than the majority of even certified techs in the US. The US med tech programs dropped histo decades ago. I'm not sure why. Pathology labs certainly benefitted financially because it allowed them to hire literally anybody to do the work. > > But even in the US the med tech schools are declining due to lack of enrollment. Probably due to automation in laboratories they just don't need as many people. > > Tim Morken > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping > Sent: Friday, May 25, 2012 4:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] certification of histotechnologists > > Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri May 25 12:24:40 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri May 25 12:24:52 2012 Subject: [Histonet] IHC for p21, p27, and RB Message-ID: <4FBF8818.7400.0077.1@harthosp.org> What has been people's experience with immunohistochemical staining for p21, p27, and Retinoblastoma (RB) gene protein on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From ibernard <@t> uab.edu Fri May 25 12:31:14 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Fri May 25 12:31:23 2012 Subject: [Histonet] certification of histotechnologists In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B5187DE7@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8B5187DE7@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: 1. The military (US Air Force) even followed this approach; maybe in alignment with the civilian sector, with a purpose of cost savings or efficiency (by eliminating the med-lab foundation before histology). However, I would like to think that they viewed histopathology as a stand-alone needed specialty that required more time to focus on skill development. For unlike med-lab, our profession is a truly an "art and science". When I received my histopathology training via the military in 1994 at the AFIP at WRAMC, all future histotechnicians had to complete medical-laboratory school. Now there is no requirement to complete med-lab school. Nevertheless, I was grateful for the med-lab foundation. Although my passion is grand scale healthcare administration, I love this field and will always try to improve and maintain my SKA in it. Hence, is the reason why I'm studying for my HTL. This is more than a job, it is a profession and thus a career. In the military (also a profession and career), we learn that for a job to be a profession or career it has to have the following attributes: - High degree of generalized and systematic knowledge- from Fixation, Processing, Specials, Immuno, Ground Histology, etc. - Primary orientation to the community interest rather than to individual self interest- we are key to patient care; i.e. we produce a product that the pathologist relies on to make patient diagnosis. - A high degree of self control of behavior through codes of ethics and voluntary associations: Ours are establish like organization like NSH, ASCP, CLIA, FDA etc. - A system of rewards or a set of symbols of work achievement. Our HT, HTL certifications are part of this. Bottom-line: our profession has a body of theory and specialized knowledge; a service orientation and a distinct subculture. So be encouraged my colleagues, be proud and know that our profession is significant to the field of medicine. I would highly suggest if you ever have the chance at a state or national Histotechnology event to hear the lecture from a pathologistd about our professional culture and the history of our profession. It will instill major professional pride. V/r IRB Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, May 25, 2012 10:56 AM To: Janet Keeping; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification of histotechnologists Janet brings up an interesting point. The rest of the world (ie, besides US) has histo as part of the med tech program and then they specialize in their final year. I have worked with techs from many other countries and in general are far more knowledgeable than the majority of even certified techs in the US. The US med tech programs dropped histo decades ago. I'm not sure why. Pathology labs certainly benefitted financially because it allowed them to hire literally anybody to do the work. But even in the US the med tech schools are declining due to lack of enrollment. Probably due to automation in laboratories they just don't need as many people. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Friday, May 25, 2012 4:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri May 25 12:40:43 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri May 25 12:40:47 2012 Subject: [Histonet] IHC for p21, p27, and RB In-Reply-To: <4FBF8818.7400.0077.1@harthosp.org> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5E47@SBS2K8.premierlab.local> Richard We just started working on a IHC protocol for p21 we tried the antibody from Dako, we looked at 1:25 and 1:50 at one hour RT incubation with pH9 retreival on some colon CA blocks, stain is specific to the nuclei with no background so far, but that's about all we have done, we were going to try an overnight incubation just to see what happens, overall we are not seeing a large amount of nuclei stain. It cross reacts with rat - that's the species we are working with. The rat samples stained but only a few nuclei. Both samples were formalin fixed. For p27 we have run this antibody just a couple times and it seems to work fine in our hands with FFPE tissues, we use human tonsil as a control. We used the one from Santa Cruz since our target was also rat. It worked well in both the human tonsil sample and the rat ovarian tumor we stained. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, May 25, 2012 11:25 AM To: Histonet Subject: [Histonet] IHC for p21, p27, and RB What has been people's experience with immunohistochemical staining for p21, p27, and Retinoblastoma (RB) gene protein on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Fri May 25 12:41:08 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri May 25 12:41:13 2012 Subject: [Histonet] Re: Elastic Stain Message-ID: <9F3CFEE76E51B64991C7485270890B4009F49868@EX5.lj.gnf.org> Hi Jim, You said "We tried to use the H&E as a counterstain bit it is still not where he wants it to be." What is the H&E counterstain lacking that your pathologist wants to see? Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From Marilyn.A.Weiss <@t> kp.org Fri May 25 13:00:14 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri May 25 13:00:24 2012 Subject: [Histonet] AUTO: I will be out the afternoon of 5/25/2012 and be back 6/4 early Tuesday morning Message-ID: I will be out of the office starting 05/25/2012 and will not return until 06/04/2012. If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. If this is concerning a block, please call the department at 619-528-6801 or tie line 8-280-6801 Thank you. Note: This is an automated response to your message "Histonet Digest, Vol 102, Issue 33" sent on 5/25/2012 9:38:38. This is the only notification you will receive while this person is away. From tjasper <@t> copc.net Fri May 25 13:46:49 2012 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri May 25 13:46:57 2012 Subject: [Histonet] certification of histotechnologists References: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A216@EXC-MBX3.cfs.le.ac.uk> Message-ID: <90354A475B420441B2A0396E5008D49692C0C1@copc-sbs.COPC.local> Janet and Richard, To answer your question...to my knowledge this has not been given consideration in the U.S.(I could be wrong). Perhaps you could explain this concept a little bit. I personally have a hard time understanding how an AP discipline (histology) is taught, mastered well enough and certified alongside clinical lab disciplines (microbiology, hematology, blood banking and general lab). To me this seems a daunting task. Am I to understand that in Canada and the UK someone certified in your medical laboratory programs is able to bounce around and work in the clinical labs and then be expected to show up in histology and work at a competent level there as well? I worked in a lab were an MLT came and helped us out now and then because she knew a little about histology. She did not cut sections too well and one day severely filleted her finger. Also, in a previous supervisory position I was asked by the general lab for help in flow cytometry from my tech specialist. He spent time down there helping out and worked in my lab as well (IHC specialist). His mind was about fried after 6 months or so and I felt it was an unfair and unrealistic expectation for him to perform at a high level in both areas. Maybe I'm not understanding this correctly. I believe the standard view in the US is that Histology and Cytology are close relatives in the world of anatomic path. The other medical lab disciplines, microbiology, hematology, BB, etc., have people floating around that understand the instrumentation and objectives of those areas. Seems it's fairly common for histologists here to assist and do testing for cytology (a bit of heme as well). Again maybe I just don't get the concept. Especially as our discipline develops and we find ourselves moving into the world of molecular pathology; not to mention the other areas of histology outside of human clinical applications. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Bend, Oregon 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, May 25, 2012 5:36 AM To: 'Janet Keeping'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification of histotechnologists Same in UK, I have never understood why the separation in the Land of the Free. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: 25 May 2012 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri May 25 14:24:44 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri May 25 14:28:45 2012 Subject: [Histonet] IHC for p21, p27, and RB In-Reply-To: <4FBF8818.7400.0077.1@harthosp.org> References: <4FBF8818.7400.0077.1@harthosp.org> Message-ID: Calbiochem p21 (1:100) and Cell Signaling for the p27 (1:250) We use the Dako Flex Reagents on the autostainer. Low pH retrieval. I have more detailed protocol if you need it. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Friday, May 25, 2012 1:24 PM To: Histonet Subject: [Histonet] IHC for p21, p27, and RB What has been people's experience with immunohistochemical staining for p21, p27, and Retinoblastoma (RB) gene protein on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Fri May 25 16:17:05 2012 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Fri May 25 16:17:14 2012 Subject: [Histonet] Not requiring HT Certification) In-Reply-To: References: Message-ID: <5412D37B-8DAB-41D3-8AAA-836E09A0CC70@gmail.com> Toysha - well said! Thank you for being brave enough in your response to counter balance the conversation. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF SF CA 94103 On May 25, 2012, at 6:25 AM, Mayer,Toysha N wrote: > > > Like everyone else, I was going to keep quiet, but I can't. > I am sensitive to those downgrading us who took the OJT route. > I did the OJT route, had a BS in Biology, tried for Veterinary School, but that wasn't so. What do I do with all of this Science? As part of my Pre-Med curricula had to take histology, loved it. Had no idea that it was a paying field and such. Had I known, I would have applied to a school as a backup. It took me a while, but when I was hired as a tech, I did not excel quickly (Cheryl remember my mistakes), but I learned and had patient coworkers. > Move on down the line several years and another coworker questioned my knowledge of the chemistry behind a stain, and all of a sudden all of my organic and biochem can running out of my mouth. I didn't realize that I really knew all of that. I had always felt a little disadvantaged because I did not get formal training through a school, just studied and passed the HT with the help of my coworkers (shout out to LSU VetPath). > There is nothing wrong with OJT for Biology majors. They should have the basic background to understand the chemistries and processes behind why we do what we do. With the modernization of technologies and procedures in the histo lab some formal education is needed. There are many ways to receive this education, it can be online, or in person. It all depends on the learner. > To overcome the stigma we should continue with some of the things that are now in place to stabilize the training of our successors (yes we all are going to have to retire one day). A continued push for formal training, promotion of the field, professionalism by our colleagues, and respect from the customers (pathologists, patients, and gen lab personnel). > In order to facilitate change for respect, we must first present a unified front. > I know good techs with no certification, I know bad techs with certification. We all do. > Last year I celebrated my 20th year in histo and never thought I would be where I am. I never wanted to do research, and didn't like management (don't like telling grown folks what to do), but I love teaching. It helps me to learn the theory behind what I do and apply it better. There are so many people who can do histo whether it is routine, special procedures, or management. > We shouldn't look down on those who took the OJT route, sometimes they just don't know about a formal program (like me). You never know where the next great manager, director or tech is coming from, so don't count them out. > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 24 May 2012 17:20:41 +0000 > From: joelle weaver > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Jon There is a route with associates and training I believe. > Of course I can't speak for the BOC, and I am sure that you want to help your employees as much as you can. I do see your point about the similarities in tasks. My thought would be that the exam eligibility states that they have to have recent experience in fixation, embedding, microtomy, and staining (histology) and the associated theory knowledge. EM is on the exam study topics, but also with the theory/experience for all those routine histological techniques, is how I read it. Take a look at the exam outlines, that should give you an idea of the scope. Ascp.org "get certified". As I have been told, they want to cover the widest possible scope of roles histologists can perform, which could include EM, but not only that. If they don't have exposure to regular histology I think that it might be hard for to feel prepared for the regular HT or HTL exams. That's just my opinion, based on what I have observed and also the pass rates ( ~ 65%), for people even with training/experience- there could be an exceptional person out there. I can understand not wanting to get buried in doing a whole HT curricula ( believe me, I do). How about the option of having cross training in a histology lab? Do you have routine histology on site or a nearby lab? The best advice I can give is to go to the website and carefully read the requirments to see how your employees might fit in. If you want to provide the theory without having to do the curricula, there are on line programs out there which can supplement OJT and a supportive mentor and organization. I have seen this work successfully with motivated people with the ability to have hands on practice alongside. I suggest the NSH site which lists the accredited programs or the NAACLS site which has a search for programs, if that would help. As far as employability, my opinion is that it would certainly open up options for your employees to also have skills in routine histology make them more valuable to your organization, and I would think certification would be even more helpful to them as far as options. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) >> From: jkrupp@deltacollege.edu >> Date: Thu, 24 May 2012 09:53:21 -0700 >> CC: histonet@lists.utsouthwestern.edu >> To: joelleweaver@hotmail.com >> >> >> On May 24, 2012, at 9:25 AM, joelle weaver wrote: >> >>> >>> I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. >> >> OK, so I have a question. >> >> We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. >> >> So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? >> >> Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. >> >> Jon >> >> Jonathan Krupp >> Delta College >> 5151 Pacific Ave. >> Box 212 >> Stockton, CA 95207 >> 209-954-5284 >> jkrupp@deltacollege.edu >> >> Find us on Facebook @ >> Electron Microscopy at SJ Delta College >> >> >> >> >> >> > > > ------------------------------ > > Message: 2 > Date: Thu, 24 May 2012 17:28:10 +0000 > From: joelle weaver > Subject: RE: [Histonet] RE: Not requiring HT Certification > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Yes I was referring to the HS and OJT training route. There is confusion about the degree + OJT and HS + OJT. Yes, that was my understanding also of the drivers, I just could not recall where I read/heard that. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: Timothy.Morken@ucsfmedctr.org >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 24 May 2012 09:56:25 -0700 >> Subject: [Histonet] RE: Not requiring HT Certification >> >> " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." >> >> Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. >> >> If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. >> >> >> Tim Morken >> >> > ******************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri May 25 18:39:01 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri May 25 18:39:06 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <2344.208.62.167.196.1337963716.squirrel@webmail.realpages.com> References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> <1337944999.87501.YahooMailNeo@web112306.mail.gq1.yahoo.com> <2344.208.62.167.196.1337963716.squirrel@webmail.realpages.com> Message-ID: Jay did not make the monkey comment. Maybe I could have made that more clear. Scott Lyons made the monkey comment, not Jay. Jay does not use the m word to refer to friends and colleagues. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From b427297 <@t> aol.com Sat May 26 11:03:01 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Sat May 26 11:03:26 2012 Subject: [Histonet] certification of histotechnologists In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A216@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A216@EXC-MBX3.cfs.le.ac.uk> Message-ID: <8CF097028975B0F-8FC-1798D@webmail-m042.sysops.aol.com> I have a new topic along this thread - seems to be generating a lot of interest. What, if any, histology group, such as NSH in the US - is available in Europe? Or any other support group for that matter. I have colleagues in Germany who would greatly benefit from being able to interact with other histotechs who are closer than ME. (Chicago area) Any suggestions will be appreciated. Also, are there certifying agencies out there like ASCP here? Jackie O' -----Original Message----- From: Edwards, Richard E. To: 'Janet Keeping' ; histonet Sent: Fri, May 25, 2012 7:36 am Subject: RE: [Histonet] certification of histotechnologists Same in UK, I have never understood why the separation in the Land of the Free. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: 25 May 2012 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gonavy2003 <@t> gmail.com Sat May 26 11:52:14 2012 From: gonavy2003 <@t> gmail.com (Rick Tiefenauer) Date: Sat May 26 11:52:17 2012 Subject: [Histonet] Unregistered techs Message-ID: Histonetters, I see this subject tends to illicit strong sentiments from professionals who are impacted or have an impact on HT/HTL's (sort of everyone on the net)?. I am still in school, but I want to fully understand how training, certification, and registration work for HT/HTL?s.? I realize that ASCP certification is voluntary, and that some States require some sort of license or certification, but I?ve never heard of a ?Registry? for HT/HTL?s. -The way I understand through what I?ve been taught at school is that Histology is the study of tissue, And that... -To study tissue there is another science that prepares specimens so they can be studied. And that... -There is a final sequence ?Quality Control? that verifies the science that prpares specimens is properly done so the tissue can be studied. And that? In order for this all to happen successfully and consistently, the HT/HTL's make sure that during the whole preparation process, safety is observed, proper adherence to federal and state regulations maintained, plus train other technicians to do the same, and much more. If I understood it all correctly I can?t help but wonder: If HT/HTL's do all of this crucial preparation work to make sure specimens are acceptable for precise microscopic identification of cells, tissue type, diagnosis of disease, and other needs: "Why wouldn't we want to have some method that can gage a set of basic skills to indicate a level of competency that HT/HTL's should initially have, in order to enter the field of work that can effect so many people either directly or indirectly?" And also... "Wouldn?t having NAACLS accredited training and ASCP Certifications serve to gage those basic skills?" And also? "Would gaging basic skills have a positive impact on the quality of patient care and the efficiency of the HT/HTL?s impact on labs?? I'm not sure but...this fall? when I complete my NAACLS accredited degree program, and voluntarily take the ASCP HTL certification exam, my future employer will be able to expect a certain level of competency that I hope to have established through training and certification. Rick T. From pathlocums <@t> gmail.com Sat May 26 12:21:18 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Sat May 26 12:21:22 2012 Subject: [Histonet] Unregistered techs Message-ID: <4103196765429318503@unknownmsgid> Well said. Everyone should aim for certification. Those that don't will offer myriad excuses, but it should be the standard. With any luck it will be required one day (likely will). Whether OJT or college trained, all should prove competency and the best way is through ASCP certification. Sent from my Windows Phone From: Rick Tiefenauer Sent: 5/26/2012 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered techs Histonetters, I see this subject tends to illicit strong sentiments from professionals who are impacted or have an impact on HT/HTL's (sort of everyone on the net)?. I am still in school, but I want to fully understand how training, certification, and registration work for HT/HTL?s.? I realize that ASCP certification is voluntary, and that some States require some sort of license or certification, but I?ve never heard of a ?Registry? for HT/HTL?s. -The way I understand through what I?ve been taught at school is that Histology is the study of tissue, And that... -To study tissue there is another science that prepares specimens so they can be studied. And that... -There is a final sequence ?Quality Control? that verifies the science that prpares specimens is properly done so the tissue can be studied. And that? In order for this all to happen successfully and consistently, the HT/HTL's make sure that during the whole preparation process, safety is observed, proper adherence to federal and state regulations maintained, plus train other technicians to do the same, and much more. If I understood it all correctly I can?t help but wonder: If HT/HTL's do all of this crucial preparation work to make sure specimens are acceptable for precise microscopic identification of cells, tissue type, diagnosis of disease, and other needs: "Why wouldn't we want to have some method that can gage a set of basic skills to indicate a level of competency that HT/HTL's should initially have, in order to enter the field of work that can effect so many people either directly or indirectly?" And also... "Wouldn?t having NAACLS accredited training and ASCP Certifications serve to gage those basic skills?" And also? "Would gaging basic skills have a positive impact on the quality of patient care and the efficiency of the HT/HTL?s impact on labs?? I'm not sure but...this fall? when I complete my NAACLS accredited degree program, and voluntarily take the ASCP HTL certification exam, my future employer will be able to expect a certain level of competency that I hope to have established through training and certification. Rick T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat May 26 16:00:23 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat May 26 16:00:30 2012 Subject: AW: [Histonet] certification of histotechnologists In-Reply-To: <8CF097028975B0F-8FC-1798D@webmail-m042.sysops.aol.com> References: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A216@EXC-MBX3.cfs.le.ac.uk> <8CF097028975B0F-8FC-1798D@webmail-m042.sysops.aol.com> Message-ID: <001e01cd3b82$91e28060$b5a78120$@gmx.at> Hi Jackie, I didn't read the whole topic posted, perhaps there's a repeat in my answer. German training as "MTA" (medical technical assistent) includes histology and histotechnolgy. So they are definitly certified in their profession with a diploma. I think this kind of education is rather similar to the Canadian way. Also in Austria, where I come from, we have the training as Biomedical Scientist in the third education sector, now ending with a Bachelor and thesis, with theoretical and practical histotechnology. Theoretically there exists a law that excludes working with patients' clinical specimens to our profession. But like everywhere cheaper persons are trained on the job and supervised by hopefully one BMS. If a pathologist takes the responsibility he may employ anyone, although he/she has not the specified technical training (doctor and employer). The registration is another issue. People taking care of patients of any profession should be registered, because on one hand the profession and on the other hand patients should be protected against bad lab practice. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jackie O'Connor Gesendet: Samstag, 26. Mai 2012 18:03 An: ree3@leicester.ac.uk; keeping.janet@gmail.com; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] certification of histotechnologists I have a new topic along this thread - seems to be generating a lot of interest. What, if any, histology group, such as NSH in the US - is available in Europe? Or any other support group for that matter. I have colleagues in Germany who would greatly benefit from being able to interact with other histotechs who are closer than ME. (Chicago area) Any suggestions will be appreciated. Also, are there certifying agencies out there like ASCP here? Jackie O' -----Original Message----- From: Edwards, Richard E. To: 'Janet Keeping' ; histonet Sent: Fri, May 25, 2012 7:36 am Subject: RE: [Histonet] certification of histotechnologists Same in UK, I have never understood why the separation in the Land of the Free. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: 25 May 2012 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Sun May 27 09:46:42 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Sun May 27 09:46:47 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: Message-ID: <1338130002.33458.YahooMailNeo@web120605.mail.ne1.yahoo.com> Good stuff. In the "old" days, 36 years ago for me,?taking the HT(ASCP) exam?it was said?that you were "registered" by the ASCP, because the designation HT?is given by the Board of Registry of the ASCP. Many of the NEW folks use the?word "certified". After a total of?39 years (3 years was training before?you were eligible)?I still only use registered by the ASCP / Licensed by the State of Florida?and call myself a "histologist". ? In those days ( long before the Internet), you took your ASCP exam (HT's, MT's, CT's, BT's, MLT's)?at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you?needed to drive 100 miles or further?to get to the?examining college?on March 15 OR August 15th, (the only dates it was given)?that's what you did.?Getting into the exam auditorium?before you were allowed to "sit" (that's what it was called)?for the exam, was a challenge. You had to prove that you were who you said you were or you?were not?getting in. Once those guarded doors were closed -?they were CLOSED! I saw several folks crying outside?the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately,?not so today. So you?can see why for us?older techs, if you were?HT(ASCP) it really meant something. Unfortunately, not so today.? ? Yours, Dave Histonetters, I see this subject tends to illicit strong sentiments from professionals who are impacted or have an impact on HT/HTL's (sort of everyone on the net)?. I am still in school, but I want to fully understand how training, certification, and registration work for HT/HTL?s.? I realize that ASCP certification is voluntary, and that some States require some sort of license or certification, but I?ve never heard of a ?Registry? for HT/HTL?s. -The way I understand through what I?ve been taught at school is that Histology is the study of tissue, And that... -To study tissue there is another science that prepares specimens so they can be studied. And that... -There is a final sequence ?Quality Control? that verifies the science that prpares specimens is properly done so the tissue can be studied. And that? In order for this all to happen successfully and consistently, the HT/HTL's make sure that during the whole preparation process, safety is observed, proper adherence to federal and state regulations maintained, plus train other technicians to do the same, and much more. If I understood it all correctly I can?t help but wonder: If HT/HTL's do all of this crucial preparation work to make sure specimens are acceptable for precise microscopic identification of cells, tissue type, diagnosis of disease, and other needs: "Why wouldn't we want to have some method that can gage a set of basic skills to indicate a level of competency that HT/HTL's should initially have, in order to enter the field of work that can effect so many people either directly or indirectly?" And also... "Wouldn?t having NAACLS accredited training and ASCP Certifications serve to gage those basic skills?" And also? "Would gaging basic skills have a positive impact on the quality of patient care and the efficiency of the HT/HTL?s impact on labs?? I'm not sure but...this fall? when I complete my NAACLS accredited degree program, and voluntarily take the ASCP HTL certification exam, my future employer will be able to expect a certain level of competency that I hope to have established through training and certification. Rick T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Rick Tiefenauer To: histonet@lists.utsouthwestern.edu Sent: Saturday, May 26, 2012 12:52 PM Subject: Re: [Histonet] Unregistered techs From wilson6848 <@t> yahoo.com Sun May 27 10:19:10 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Sun May 27 10:19:14 2012 Subject: [Histonet] Renal Core Biopsy Message-ID: <1338131950.21103.YahooMailNeo@web120902.mail.ne1.yahoo.com> ? ? Hello, ????????? I would like to know how you guys receive your renal core biopsy. Our Pathologists said they were having problem getting the biopsy out of the little renal biopsy bottle sent from the Radiology Dept. ?? Thanks, Wilson From amosbrooks <@t> gmail.com Sun May 27 10:28:37 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun May 27 10:28:39 2012 Subject: [Histonet] Embedding Message-ID: Hi, Any chance it is from your reservoir? Sometimes the paraffin is not filtered very well and there is some junk that collects in the chamber. (Yes I'm looking at you Paraplast!) Also is the reservoir you keep your cassettes in prior to embedding clean? Perhaps junk on your tampers? Amos On Sat, May 26, 2012 at 1:00 PM, wrote: > Message: 1 > Date: Fri, 25 May 2012 13:02:29 -0400 (EDT) > From: Ann Specian > Subject: [Histonet] Embedding > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > We are having a problem with floaters in our blocks which occur during > embedding. We have multiple forceps which are placed in heated wells and > each cassette is embedded with a new forcep. We also wipe with a gauze, > but we are still getting floaters embedded in the cassette from time to > time. > > Does anyone do anything else to prevent this? > Thank you, Ann > From jrsmallwood <@t> bell.net Sun May 27 15:46:27 2012 From: jrsmallwood <@t> bell.net (John Smallwood) Date: Sun May 27 15:46:40 2012 Subject: [Histonet] Leitz 1512 Message-ID: Does anyone have a resource for parts for this 40 year old microtome ?? Thanks John Smallwood From info <@t> morphisto.de Mon May 28 02:15:28 2012 From: info <@t> morphisto.de (Morphisto GmbH (Kundenbetreuung)) Date: Mon May 28 02:15:43 2012 Subject: [Histonet] Leitz 1512 In-Reply-To: References: Message-ID: <6F705959-303E-453B-8AD3-41490D1C058C@morphisto.de> Hello John, which kind of resource parts do you need? We have two of this very good microtomes in our lab, but we recently do not use them very often. Best regards Michael Am 27.05.2012 um 22:46 schrieb John Smallwood: > Does anyone have a resource for parts for this 40 year old microtome ?? > Thanks > John Smallwood > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 60 Telefax: 069 / 989 72 1100 E-Mail: info@morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From billions1998 <@t> hotmail.com Mon May 28 02:19:53 2012 From: billions1998 <@t> hotmail.com (Sinoera Tech) Date: Mon May 28 02:20:05 2012 Subject: [Histonet] Re:Alcian Yellow In-Reply-To: <6F705959-303E-453B-8AD3-41490D1C058C@morphisto.de> References: <6F705959-303E-453B-8AD3-41490D1C058C@morphisto.de> Message-ID: Dear Histonetters, We are offering Alcian Yellow, Alcian Blue 8GX. Looking forward to hearing from you. Kind Regards SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road, Suzhou New & Hi-Tech District, 215011 China Tel: 0086 512 68246939 Fax: 0086 512 68258994 Inquiries: billions1998@hotmail.com General Questions: sinoerachem@sina.cn http://www.sinoeratech.com http://www.sinoerachem.com From ree3 <@t> leicester.ac.uk Mon May 28 03:54:24 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon May 28 03:59:21 2012 Subject: [Histonet] certification of histotechnologists In-Reply-To: <8CF097028975B0F-8FC-1798D@webmail-m042.sysops.aol.com> References: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A216@EXC-MBX3.cfs.le.ac.uk> <8CF097028975B0F-8FC-1798D@webmail-m042.sysops.aol.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A227@EXC-MBX3.cfs.le.ac.uk> During the time of the British Empire, many of the member countries/colonies adopted many British institutions, including the British model of organizing medical laboratories and the associated training .I believe the link is still maintained today in countries such as Australia, New Zealand, Malaysia and Hong Kong, in fact the UK professional body (Institute of BioMedical Science) used to supervise their qualification processes, maybe they still do?, perhaps somebody could inform us?. From: Jackie O'Connor [mailto:b427297@aol.com] Sent: 26 May 2012 17:03 To: ree3@leicester.ac.uk; keeping.janet@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] certification of histotechnologists I have a new topic along this thread - seems to be generating a lot of interest. What, if any, histology group, such as NSH in the US - is available in Europe? Or any other support group for that matter. I have colleagues in Germany who would greatly benefit from being able to interact with other histotechs who are closer than ME. (Chicago area) Any suggestions will be appreciated. Also, are there certifying agencies out there like ASCP here? Jackie O' -----Original Message----- From: Edwards, Richard E. To: 'Janet Keeping' ; histonet Sent: Fri, May 25, 2012 7:36 am Subject: RE: [Histonet] certification of histotechnologists Same in UK, I have never understood why the separation in the Land of the Free. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: 25 May 2012 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification of histotechnologists Just curious if any consideration has been given to including Histotechnology in your medical laboratory programs as we do in Canada? our graduates are certified for 5 different careers and shortages in one particular laboratory does not seem to be a problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From itai.moshe <@t> mail.huji.ac.il Mon May 28 06:24:31 2012 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Mon May 28 06:24:39 2012 Subject: [Histonet] Staining paraffin blocks of evans blue treated mice with Sirius red Message-ID: Hi Histonets. Can i stain slides from praffin blocks of Evans blue treated mice with Sirius red ? Does the Sirius red might be effected from the Evans blue treatment? Have someone done it before, or have a reference for it ? Thank you all, Itai From mucram11 <@t> comcast.net Mon May 28 07:29:42 2012 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon May 28 07:30:14 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <1338130002.33458.YahooMailNeo@web120605.mail.ne1.yahoo.com> Message-ID: <979416964.133726.1338208182892.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> You forgot one thing.? We had to pass a practical examination of stains they told you to do and pass before we could sit for the written!? That went away with all the automated equipment and many who OJT now and even some schools don't do a hand staining specal stain sequence from scratch.??Or what all the ex tras ones we did by hand are as the list is s o extensive.? IHC is taking some of the old stains away and is a better method for some disease processes.? However ; we still have some older patholgoist wh o want to see stains we don't do or can't due to mercury or other components we are not allowed to dispose of without a permit or extra expense to get r id of.? We have automated and hand staining with some of us older histologist still enjoying the occassional hand stain technique. Pam Marcum ----- Original Message ----- From: "David Kemler" To: "Fellow HistoNetters" Sent: Sunday, May 27, 2012 9:46:42 AM Subject: Re: [Histonet] Unregistered techs Good stuff. In the "old" days, 36 years ago for me,?taking the HT(ASCP) exam?it was said?that you were "registered" by the ASCP, because the designation HT?is given by the Board of Registry of the ASCP. Many of the NEW folks use the?word "certified". After a total of?39 years (3 years was training before?you were eligible)?I still only use registered by the ASCP / Licensed by the State of Florida?and call myself a "histologist". ? In those days ( long before the Internet), you took your ASCP exam (HT's, MT's, CT's, BT's, MLT's)?at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you?needed to drive 100 miles or further?to get to the?examining college?on March 15 OR August 15th, (the only dates it was given)?that's what you did.?Getting into the exam auditorium?before you were allowed to "sit" (that's what it was called)?for the exam, was a challenge. You had to prove that you were who you said you were or you?were not?getting in. Once those guarded doors were closed -?they were CLOSED! I saw several folks crying outside?the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately,?not so today. So you?can see why for us?older techs, if you were?HT(ASCP) it really meant something. Unfortunately, not so today.? ? Yours, Dave Histonetters, I see this subject tends to illicit strong sentiments from professionals who are impacted or have an impact on HT/HTL's (sort of everyone on the net)?. I am still in school, but I want to fully understand how training, certification, and registration work for HT/HTL?s.? I realize that ASCP certification is voluntary, and that some States require some sort of license or certification, but I?ve never heard of a ?Registry? for HT/HTL?s. -The way I understand through what I?ve been taught at school is that Histology is the study of tissue, And that... -To study tissue there is another science that prepares specimens so they can be studied. And that... -There is a final sequence ?Quality Control? that verifies the science that prpares specimens is properly done so the tissue can be studied. And that? In order for this all to happen successfully and consistently, the HT/HTL's make sure that during the whole preparation process, safety is observed, proper adherence to federal and state regulations maintained, plus train other technicians to do the same, and much more. If I understood it all correctly I can?t help but wonder: If HT/HTL's do all of this crucial preparation work to make sure specimens are acceptable for precise microscopic identification of cells, tissue type, diagnosis of disease, and other needs: "Why wouldn't we want to have some method that can gage a set of basic skills to indicate a level of competency that HT/HTL's should initially have, in order to enter the field of work that can effect so many people either directly or indirectly?" And also... "Wouldn?t having NAACLS accredited training and ASCP Certifications serve to gage those basic skills?" And also? "Would gaging basic skills have a positive impact on the quality of patient care and the efficiency of the HT/HTL?s impact on labs?? I'm not sure but...this fall? when I complete my NAACLS accredited degree program, and voluntarily take the ASCP HTL certification exam, my future employer will be able to expect a certain level of competency that I hope to have established through training and certification. Rick T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Rick Tiefenauer To: histonet@lists.utsouthwestern.edu Sent: Saturday, May 26, 2012 12:52 PM Subject: Re: [Histonet] Unregistered techs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From enarvaez <@t> cblpath.com Mon May 28 08:31:46 2012 From: enarvaez <@t> cblpath.com (Edison Narvaez) Date: Mon May 28 08:31:50 2012 Subject: [Histonet] Unsuscribe Message-ID: <85073123-E8C0-4C3D-A027-6D97A02488EF@cblpath.com> From one_angel_secret <@t> yahoo.com Mon May 28 12:41:29 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon May 28 12:41:38 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <2344.208.62.167.196.1337963716.squirrel@webmail.realpages.com> References: <3C8C50D3-470C-43E0-B5AD-1DCB39FA26BF@yahoo.com> <1337944999.87501.YahooMailNeo@web112306.mail.gq1.yahoo.com> <2344.208.62.167.196.1337963716.squirrel@webmail.realpages.com> Message-ID: <100DC654-9E83-4F3B-929A-E7F2F9D2EC88@yahoo.com> And you are a wonderful tech. And I'm so proud of you for continuing your education. Anyone who gets mentored by you will be very lucky. Hope your having a great day. Luv ya Sent from my iPhone On May 25, 2012, at 12:35 PM, "Nicole Tatum" wrote: >> LOL, I dont have much to say about this one. Like you Kim I have a fl HTL > license and an HT ASCP. I have two classes left before I complete my BSH > and will be able to sit for my ASCP HTL. I have 12yrs of experience but > that is not reconginized in the eyes of licensure for ASCP. I do believe > my skill level should determine my licensure status not the degree I > hold. Because I promise every class im taking rt now means nothing > towards my field. The word histology has not even be written in any of > the books im studying. I think OJT techs are just as qualified as any > person who completed an online programs, if not more so. I completed an > online histology program and learned my skills from other people in the > bizz with alot of experience who were willing to train me and be patient. > Never one time did I question their, experience, licensure, or training. > The OJTs carried Histology through the transisition of licensure and > passed the skills and knowlegde on to us young bucks to carry the torch. > > That is all...lol > Nicole Tatum HT ASCP (hahaha soon to be HTL ASCP) :)~~~~~~~ > > > > > > > LOL, I was waiting for this thread to turn into a fist fight. I knew it >> would. >> >> A Fl Licensed HTL is someone who oviously had to take more test than a >> ASCP certified tech if they got it from 19 years ago. How do I know, I'm >> one of those who had to take all those test way back then. Florida is >> pretty stringent who can do what with what. Not as much as they used to >> be, because now the only route is through ASCP. You will not work in a >> hospital as a histologist without a Fl License. Maybe in a exclusive >> provider private office, but that is the only exception. >> >> As far as Monkeys. Whew! I think that's whats wrong with our profession, >> one thing anyway. Too many people veiw it that way. >> >> Personally I wish the Histo programs would go back to teaching on campus >> with using the MLT course comparison. Not to bash anyone who is either >> involved or has been involved with the internet programs that have popped >> up everywhere, but I'm not seeing the same level of technical skill or >> knowledge come out of these programs. I'm sure there are many super >> students who do get it, but many times they are pushed through on thier >> short rotations and used as gophers etc and dont have the skills even >> sometimes to cut a block. And forget about knowing what a good control for >> a GMS is. >> >> I'd like to see our profession go back to min AS degrees in Histology. The >> certification only have hurt us and the pay scale is changing I feel >> because of it. >> >> Also, if you really want to promote our field and improve it, be >> supportive of your state going to a license for our practice. ASCP >> supports states requiring license. >> >> Thats my 2cents for the day. Happy Friday! :) >> >> Kim D >> >> >> ________________________________ >> From: William Chappell >> To: Davide Costanzo >> Cc: histonet >> Sent: Thursday, May 24, 2012 7:02 PM >> Subject: Re: [Histonet] Unregistered techs >> >> I have respected Jay's input in the past, but I too must say something. >> >> Without realizing it, and by stating his opinion in a horribly crass way, >> Jay has touched upon an important truism. There are two types of >> histologists, those that have a job that pays the bills, and those who >> have a career in which they thrive. Neither are better than the other, >> both are needed. I suspect, however, that the majority of Histonetters -- >> especially avid contributors are in the latter group. I know I am. >> >> Histotechs who approach histology as a job, go into work, embed, cut, >> stain and go home. they are excellent techs, but are just not committed >> to expanding the field or doing more than is needed to provide the >> pathologist with a perfect slide. Jay refers to these people as no better >> than trained monkeys. That is a horrible insult with a small (very small) >> grain of truth. One day those histologists will be replaced by a >> mechanical/robotic process. The march of progress is unstoppable. >> >> The career histologist has a much longer life span however. We analyze >> and troubleshoot problems. We understand or endeavor to learn the organic >> chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is >> made. We know more about the practice of histology than ANY pathologist. >> We invent and develop antibodies and special stains. And we conceptualize >> and perfect the instruments that will replace the first group in the >> future. >> >> Jay, that is why so many are offended. We don't do this simply because it >> is a good paycheck. We are histologists because we are professionals who >> choose this career. You may be going to a job cutting slides (which is >> great and necessary), but we are enjoying our life. >> >> Will Chappell, HTL (ASCP), QIHC, MBA >> and histologist by choice, not accident >> >> >> On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: >> >>> I'm sorry - I cannot let this rest. The comment: "we are just as much >>> needed as pathologists, blah, blah, >>> blah......." is so upsetting I cannot sit back and listen to that >>> without >>> saying something! >>> >>> Everyone, regardless of their lot in life, is a very worthwhile part of >>> the >>> whole. Let me ask you a question, since you highly undervalue humans >>> that >>> are not MD's - let's say that you are a patient at Hospital X, and you >>> go >>> in to have your toenail removed. Who plays a more important role in your >>> survival - the Podiatrist or the hospital janitor? I would argue that >>> the >>> janitor is more crucial in this instance, for if he/she fails to clean >>> up >>> the MRSA from the last patient you could conceivably die. The doctor >>> solved >>> your fungal problem, but the janitor prevented you from getting a >>> potentially life-threatening infection. Think before you speak like that >>> - >>> everyone involved in your care is critical - and, yes, sometimes the >>> doctor >>> is not the most important person when it comes to keeping you alive and >>> well! >>> >>> >>> >>> >>> >>> On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren >>> wrote: >>> >>>> Scott Lyons slnj07@yahoo.com >>>> >>>> Give me a break, HTs and HTLs do not make diagnoses or treat patients. >>>> I >>>> am a registered HT and a Florida licensed HTL with 19 years experience, >>>> I've done it all in the lab. I believe the certification and licensure >>>> of >>>> techs is a scam to bleed more money from people. Honestly, you can >>>> train a >>>> monkey to do our job. And I don't want to hear from everyone saying >>>> it's an >>>> art form, we are just as much needed as pathologists, blah, blah, >>>> blah....... I work where they are hiring people from a masters degree >>>> program for histology with certification, THEY KNOW NOTHING. Experience >>>> it >>>> where it's at, whether certified or not, get off your high horse. >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>>> Histonet@lists.utsouthwestern.edu >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> -- >>> *David Costanzo, MHS, PA (ASCP)* >>> Project Manager >>> *Blufrog Path Lab Solutions* >>> 9401 Wilshire Blvd. Ste 650 >>> Beverly Hills, CA 90212 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From pruegg <@t> ihctech.net Mon May 28 12:58:33 2012 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Mon May 28 13:00:25 2012 Subject: [Histonet] Unregistered techs Message-ID: <20120528105833.f86bd30e73b823f57b516b5451216a98.cf73b36ceb.wbe@email01.secureserver.net> Well said Will. -------- Original Message -------- Subject: Re: [Histonet] Unregistered techs From: William Chappell << href="mailto:chapcl@yahoo.com">chapcl@yahoo.com> Date: Thu, M To: Davide Costanzo <[1]pathlocums@gmail.com> Cc: histonet <[2]histonet@lists.utsouthwestern.edu> I have respected Jay's input in the past, but I too must say s omething. Without realizing it, and by stating his opinion in a horr way, Jay has touched upon an important truism. There are two typ of histologists, those that have a job that pays the bills, and those wh other, bo Histonetters -- esp group. I know I am. Hist cut, stain and committed to expanding provide the pathologist with a p people as no better than trained monkeys. with a small (very small) grain of truth. One da will be replaced by a mechanical/robotic process. The progress is unstoppable. The career histologist has a much analyze and troubleshoot problems. We understa learn the organic chemistry of stains. We know EXACTLY ho Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and specia that will replac Jay, that is why so many are offend because it is a good paycheck. We are histologi professionals who choose this career. You may be going t cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by On May 24, 2012, at 6:48 PM, Davide Costan > I'm sorry - I cannot let this rest. The comment: "we much > needed as pathologists, blah, blah, > blah.. without > > > Everyone, regardless of their lot in li part of the > whole. Let me ask you a questi humans that > are not MD's - let's sa you go > in to have your in your > survival - that the > jan clean up & doctor so > your fungal problem, but the janitor prevented you from gettin > potentially life-threatening infection. Think before you speak like that - > everyone involved in your care is critical - and, yes, the doctor > is not the most important person when it comes and > well! > > > > > > On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren <[3]jaylundgren@gmail.com> wrote: & >> Scott Lyons [4]slnj07@yah >> >> Give me a break, HTs and HTLs do not ma patients. I >> am a registered HT and a Flor experience, >> I've done it all in licensure of >> techs is can train a >&g saying it's >> art form, we are just as much needed as pathologists, blah, >> blah....... I work where they are hiring people from a m degree >> program for histology with certification, THEY KN Experience it >> where it's at, whether certified or n >> >> >> > >> >> >> >> >> & >> >> >> >> >> >> >> >> >> >> >> >> >>> [5]Histonet@lists.utsouthwestern.edu >>> [6]http://lists.utso >> __________________ >> Histonet mailing list >> >> [8]http://lists.utsouthwestern.edu/mailman/listinfo/hi >> > > > > -- > *Dav > Project Manager > *Blufrog Path > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, C > _______________________________________________ > His > [9]Histonet@lists.utsouthwestern.edu > [10]http://lists.utsouthwestern.e _______________________________ Histonet mailing list [11]Histonet@lists.utsouthwestern.edu [12]http://lists.uts References 1. 3D"mailto:pathlocums 2. file://localhost/tmp/3D"mai 3. ="mailto:jaylundgren@gmail.com" 4. 3D"mailto:slnj07@yahoo.com" 5. 3D"mailto:Histonet@lists.utsouthwest 6. file://localhost/tmp/3D"h 7. 3D"mailto:Histonet@lists.utsouthwestern.edu" 8. 3D"http://lists.utsouthwestern.edu/mai 9. 3D"mailto:Histonet@lists.utsouthwestern. 10. 3D"http://lists.=/ 11. 3D"mailto:Histonet@lis 12. file://localhost/tmp/3D" From techmana12 <@t> yahoo.com Tue May 29 00:14:49 2012 From: techmana12 <@t> yahoo.com (Dorothy Ragland-Glass) Date: Tue May 29 00:03:04 2012 Subject: [Histonet] Unregistered techs Message-ID: Dorothy R. Glass, BS,HTL(ASCP),IHC You are so right about the good old days when you would prove who you were and sit for a paper not computer exam at a medical school close to you. I sat for the HTL in 1988. I was so proud to call myself a Histologist after being trained at a School of Histotechnology and being ASCP certified. NOW you have people on the job trained just referring to themselves casually as histotects. Not histo trainees. Unfortunate for us older techs, it is very offensive considering what we went through in establishing a career not just a job. Some reference labs is focusing on hiring aids to later, after a few months train them and refer to them as histotechs. I hate it when the term is used so freely. Sinserely, frustrated HTL David Kemler wrote: >Good stuff. In the "old" days, 36 years ago for me,?taking the HT(ASCP) exam?it was said?that you were "registered" by the ASCP, because the designation HT?is given by the Board of Registry of the ASCP. Many of the NEW folks use the?word "certified". After a total of?39 years (3 years was training before?you were eligible)?I still only use registered by the ASCP / Licensed by the State of Florida?and call myself a "histologist". >? >In those days ( long before the Internet), you took your ASCP exam (HT's, MT's, CT's, BT's, MLT's)?at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you?needed to drive 100 miles or further?to get to the?examining college?on March 15 OR August 15th, (the only dates it was given)?that's what you did.?Getting into the exam auditorium?before you were allowed to "sit" (that's what it was called)?for the exam, was a challenge. You had to prove that you were who you said you were or you?were not?getting in. Once those guarded doors were closed -?they were CLOSED! I saw several folks crying outside?the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately,?not so today. So you?can see why for us?older techs, if you were?HT(ASCP) it really meant something. Unfortunately, not so today.? >? >Yours, >Dave >Histonetters, > >I see this subject tends to illicit strong sentiments from >professionals who are impacted or have an impact on HT/HTL's (sort of >everyone on the net)?. > >I am still in school, but I want to fully understand how training, >certification, and registration work for HT/HTL?s.? I realize that >ASCP certification is voluntary, and that some States require some >sort of license or certification, but I?ve never heard of a ?Registry? >for HT/HTL?s. > >-The way I understand through what I?ve been taught at school is that >Histology is the study of tissue, And that... > >-To study tissue there is another science that prepares specimens so >they can be studied. And that... > >-There is a final sequence ?Quality Control? that verifies the science >that prpares specimens is properly done so the tissue can be studied. >And that? > >In order for this all to happen successfully and consistently, the >HT/HTL's make sure that during the whole preparation process, safety >is observed, proper adherence to federal and state regulations >maintained, plus train other technicians to do the same, and much >more. > >If I understood it all correctly I can?t help but wonder: > >If HT/HTL's do all of this crucial preparation work to make sure >specimens are acceptable for precise microscopic identification of >cells, tissue type, diagnosis of disease, and other needs: > >"Why wouldn't we want to have some method that can gage a set of basic >skills to indicate a level of competency that HT/HTL's should >initially have, in order to enter the field of work that can effect so >many people either directly or indirectly?" > >And also... "Wouldn?t having NAACLS accredited training and ASCP >Certifications serve to gage those basic skills?" > >And also? "Would gaging basic skills have a positive impact on the >quality of patient care and the efficiency of the HT/HTL?s impact on >labs?? > >I'm not sure but...this fall? when I complete my NAACLS accredited >degree program, and voluntarily take the ASCP HTL certification exam, >my future employer will be able to expect a certain level of >competency that I hope to have established through training and >certification. > >Rick T. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >________________________________ > >From: Rick Tiefenauer >To: histonet@lists.utsouthwestern.edu >Sent: Saturday, May 26, 2012 12:52 PM >Subject: Re: [Histonet] Unregistered techs > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue May 29 07:50:42 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue May 29 07:50:48 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E25366D@evcspmbx3.ads.northwestern.edu> All, My point exactly. It belittles what we do (our education and training) and went through to get certified. As I voiced to a an MD the other day, it would be like him calling himself an MD without being licensed. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Ragland-Glass Sent: Tuesday, May 29, 2012 12:15 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] Unregistered techs Dorothy R. Glass, BS,HTL(ASCP),IHC You are so right about the good old days when you would prove who you were and sit for a paper not computer exam at a medical school close to you. I sat for the HTL in 1988. I was so proud to call myself a Histologist after being trained at a School of Histotechnology and being ASCP certified. NOW you have people on the job trained just referring to themselves casually as histotects. Not histo trainees. Unfortunate for us older techs, it is very offensive considering what we went through in establishing a career not just a job. Some reference labs is focusing on hiring aids to later, after a few months train them and refer to them as histotechs. I hate it when the term is used so freely. Sinserely, frustrated HTL David Kemler wrote: >Good stuff. In the "old" days, 36 years ago for me,?taking the HT(ASCP) exam?it was said?that you were "registered" by the ASCP, because the designation HT?is given by the Board of Registry of the ASCP. Many of the NEW folks use the?word "certified". After a total of?39 years (3 years was training before?you were eligible)?I still only use registered by the ASCP / Licensed by the State of Florida?and call myself a "histologist". >? >In those days ( long before the Internet), you took your ASCP exam >(HT's, MT's, CT's, BT's, MLT's)?at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you?needed to drive 100 miles or further?to get to the?examining college?on March 15 OR August 15th, (the only dates it was given)?that's what you did.?Getting into the exam auditorium?before you were allowed to "sit" (that's what it was called)?for the exam, was a challenge. You had to prove that you were who you said you were or you?were not?getting in. Once those guarded doors were closed -?they were CLOSED! I saw several folks crying outside?the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately,?not so today. So you?can see why for us?older techs, if you were?HT(ASCP) it really meant something. Unfortunately, not so today. >? >Yours, >Dave >Histonetters, > >I see this subject tends to illicit strong sentiments from >professionals who are impacted or have an impact on HT/HTL's (sort of >everyone on the net)?. > >I am still in school, but I want to fully understand how training, >certification, and registration work for HT/HTL?s.? I realize that ASCP >certification is voluntary, and that some States require some sort of >license or certification, but I?ve never heard of a ?Registry? >for HT/HTL?s. > >-The way I understand through what I?ve been taught at school is that >Histology is the study of tissue, And that... > >-To study tissue there is another science that prepares specimens so >they can be studied. And that... > >-There is a final sequence ?Quality Control? that verifies the science >that prpares specimens is properly done so the tissue can be studied. >And that? > >In order for this all to happen successfully and consistently, the >HT/HTL's make sure that during the whole preparation process, safety is >observed, proper adherence to federal and state regulations maintained, >plus train other technicians to do the same, and much more. > >If I understood it all correctly I can?t help but wonder: > >If HT/HTL's do all of this crucial preparation work to make sure >specimens are acceptable for precise microscopic identification of >cells, tissue type, diagnosis of disease, and other needs: > >"Why wouldn't we want to have some method that can gage a set of basic >skills to indicate a level of competency that HT/HTL's should initially >have, in order to enter the field of work that can effect so many >people either directly or indirectly?" > >And also... "Wouldn?t having NAACLS accredited training and ASCP >Certifications serve to gage those basic skills?" > >And also? "Would gaging basic skills have a positive impact on the >quality of patient care and the efficiency of the HT/HTL?s impact on >labs?? > >I'm not sure but...this fall? when I complete my NAACLS accredited >degree program, and voluntarily take the ASCP HTL certification exam, >my future employer will be able to expect a certain level of competency >that I hope to have established through training and certification. > >Rick T. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >________________________________ > >From: Rick Tiefenauer >To: histonet@lists.utsouthwestern.edu >Sent: Saturday, May 26, 2012 12:52 PM >Subject: Re: [Histonet] Unregistered techs > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue May 29 10:00:51 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 29 10:01:07 2012 Subject: [Histonet] Renal Core Biopsy In-Reply-To: <1338131950.21103.YahooMailNeo@web120902.mail.ne1.yahoo.com> References: <1338131950.21103.YahooMailNeo@web120902.mail.ne1.yahoo.com> Message-ID: <4FC4AC63.7400.0077.1@harthosp.org> Are you referring to biopsies done for medical renal disease or tumor? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Wilson A 5/27/2012 11:19 AM >>> Hello, I would like to know how you guys receive your renal core biopsy. Our Pathologists said they were having problem getting the biopsy out of the little renal biopsy bottle sent from the Radiology Dept. Thanks, Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tajibade <@t> echd.org Tue May 29 10:43:29 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Tue May 29 10:43:40 2012 Subject: [Histonet] IHC cost per test calculator Message-ID: Hello everyone, Is there any cost per test calculator that can be used to determine the cost per test for IHC? Thanks Tunde Ajibade CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From TNMayer <@t> mdanderson.org Tue May 29 11:23:54 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue May 29 11:24:39 2012 Subject: [Histonet] RE: Embedding Message-ID: Yes, cleaning your wells would be the next possible thing to try. Clean them out with a little xylene (or paraguard) daily. Also if your embedding unit has the removable wells, placing them upside down in the oven may be something to try. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ******************************** Message: 1 Date: Fri, 25 May 2012 13:02:29 -0400 (EDT) From: Ann Specian Subject: [Histonet] Embedding To: histonet@lists.utsouthwestern.edu Message-ID: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We are having a problem with floaters in our blocks which occur during embedding. We have multiple forceps which are placed in heated wells and each cassette is embedded with a new forcep. We also wipe with a gauze, but we are still getting floaters embedded in the cassette from time to time. Does anyone do anything else to prevent this? Thank you, Ann From TNMayer <@t> mdanderson.org Tue May 29 11:33:13 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue May 29 11:34:20 2012 Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 34 In-Reply-To: <3a296b18-3a59-4a47-a181-6333dca04df6@DCPWPRTR01.mdanderson.edu> References: <3a296b18-3a59-4a47-a181-6333dca04df6@DCPWPRTR01.mdanderson.edu> Message-ID: Well said Joelle. It is almost like choosing schools for your child, not every school will fit every child. Promotion is a good key to attracting new HT/HTL students. When I visit with students in 4 yr universities, I always ask the pre-med students what are they going to do with all of the science if they do not get into med/dental/nursing/pharmacy school. They don't even know about histo and how it can help them. Felton is right, in order to remain competitive in management and director positions we have to advance our education. It has to be us to tell us what to do and how to do it. On another note, you CSI watchers, I saw a few years back the show was assisting ACSP in promoting lab week. Great job, but on the show I saw the M.E. with a microtome, and scope at the side of the body, and he was attempting to demonstrate histology. I cringed. NSH should have talked to them about that display. House does a little better, but I don't any physicians that perform Fite stains. OK I'm off my horse now. Thanks for listening. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- ------------------------------ Message: 5 Date: Fri, 25 May 2012 17:21:49 +0000 From: joelle weaver Subject: RE: [Histonet] certification of histotechnologists To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" These are interesting points. In my experience with the educational "wing" at least some ( non-histology educators) have about the same "monkey" assessment of what knowledge and skills are needed for histology as some others. They seemed to feel that an associates was unecessary, and also feel that any med tech, ( or just about anyone) can do histology, with or without training- so why do we need to have programs at all? Plus it's a money issue- based on enrollment. So on campus programs fell away and continue to do so. The alternative was to have the students train in an off site lab and attend class on campus. Finding those labs willing to take students for clinicals was challenging. Then came not even classes on campus, but on line. That seemed to provide something for some people. It is a better alternative than nothing, and some programs seem to be good, and can provide the theory. But based on my first hand experience, I came to the conclusion that for quite a few students, it just doesn't translate well. Before I get a "flurry" of angry responses, note that some people do quite well- it just depends on the individual and where their clinical site is. I just think that more people deserve to be set up to do well, and I think the education could be more unified and consistent. I am not even "blaming" educators, they do their best with what resources they are given- I just think that those resources are often not enough. I was lucky to have training in a program on campus, with good instructors, good clinicals, and then go on to work in a great lab with people who wanted me to succeed. I never entertained that this was a substitute for actual experience, but I did feel it gave me good fundamentals to get started, and I continue to learn everyday both on my own, and from others. Whenever I get sucked into this topic, it always gets construed that I am somehow insulting people who followed different paths- I am not! Experience is always valuable.There are good and bad examples in ALL professions, no matter the individual's education, training or experience. Education and experience are both valuable. They are just different ways of learning, and hopefully can work in synergy for each person. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: keeping.janet@gmail.com; histonet@lists.utsouthwestern.edu > Date: Fri, 25 May 2012 08:55:58 -0700 > Subject: RE: [Histonet] certification of histotechnologists > CC: > > Janet brings up an interesting point. The rest of the world (ie, besides US) has histo as part of the med tech program and then they specialize in their final year. I have worked with techs from many other countries and in general are far more knowledgeable than the majority of even certified techs in the US. The US med tech programs dropped histo decades ago. I'm not sure why. Pathology labs certainly benefitted financially because it allowed them to hire literally anybody to do the work. > > But even in the US the med tech schools are declining due to lack of enrollment. Probably due to automation in laboratories they just don't need as many people. > > Tim Morken > > ******************** From pruegg <@t> ihctech.net Tue May 29 12:18:29 2012 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue May 29 12:18:35 2012 Subject: [Histonet] Unregistered techs Message-ID: <20120529101829.f86bd30e73b823f57b516b5451216a98.a613e9ad39.wbe@email01.secureserver.net> There is nothing volunteer about being ASCP certified as an HT or= HTL where I have worked for the last 35 years, all those employed as HT's = at the U of Colorado must be ASCP certified and I believe this is the case = for most other places doing hospital based Histology, work, right???? =0A =0A =0A------= -- Original Message -------- =0ASubject: Re: [Histonet] Unregistered tec= hs =0AFrom: Dorothy Ragland-Glass <[1]techmana12@yahoo.com> =0ADate: Mon, May 28, 2012 10:14 pm=0ATo: David Kemler <[2]histotalk@= yahoo.com>, Fellow HistoNetters =0A<[3]Histonet@Lists.UTSouthwestern.edu> =0A= =0ADorothy R. Glass, BS,HTL(ASCP),IHC =0A You are so right about = the good old days when you would prove who you were and sit for a paper not= computer exam at a medical school close to you. I sat for the HTL in 1988.= I was so proud to call myself a Histologist after being trained at a Schoo= l of Histotechnology and being ASCP certified. NOW you have people on the j= ob trained just referring to themselves casually as histotects. Not histo t= rainees. Unfortunate for us older techs, it is very offensive considering w= hat we went through in establishing a career not just a job. Some reference= labs is focusing on hiring aids to later, after a few months train them an= d refer to them as histotechs. I hate it when the term is used so freely.=0ASinserely, frustrated HTL =0A =0A =0ADavid Kemler <[4]histotalk@yahoo.com> wrote: =0A=0A>Good stuff. In the "old" days, 36 years ago for me, taking th= e HT(ASCP) exam it was said that you were "registered" by the ASC= P, because the designation HT is given by the Board of Registry of the= ASCP. Many of the NEW folks use the word "certified". After a total o= f 39 years (3 years was training before you were eligible) I= still only use registered by the ASCP / Licensed by the State of Florida&n= bsp;and call myself a "histologist". =0A> =0A>In those d= ays ( long before the Internet), you took your ASCP exam (HT's, MT's, CT's,= BT's, MLT's) at specially selected medical schools across the US. You= chose the one giving the exam which was closest to where you lived. If you= needed to drive 100 miles or further to get to the examinin= g college on March 15 OR August 15th, (the only dates it was given)&nb= sp;that's what you did. Getting into the exam auditorium before y= ou were allowed to "sit" (that's what it was called) for the exam, was= a challenge. You had to prove that you were who you said you were or you&n= bsp;were not getting in. Once those guarded doors were closed - t= hey were CLOSED! I saw several folks crying outside the auditorium tha= t day n 1975. Chances for cheating were eliminated at every turn. Unfortuna= tely, not so today. So you can see why for us older techs, i= f you were HT(ASCP) it really meant something. Unfortunately, not so t= oday. =0A> =0A>Yours, =0A>Dave =0A>Hist= onetters, =0A> =0A>I see this subject tends to illicit strong s= entiments from =0A>professionals who are impacted or have an impact o= n HT/HTL's (sort of =0A>everyone on the net)?. =0A> =0A>I= am still in school, but I want to fully understand how training, =0A>= ;certification, and registration work for HT/HTL=E2=80=99s. I realize= that =0A>ASCP certification is voluntary, and that some States requi= re some =0A>sort of license or certification, but I=E2=80=99ve never = heard of a =E2=80=9CRegistry=E2=80=9D =0A>for HT/HTL=E2=80=99s. = =0A> =0A>-The way I understand through what I=E2=80=99ve been taug= ht at school is that =0A>Histology is the study of tissue, And that..= . =0A> =0A>-To study tissue there is another science that prepa= res specimens so =0A>they can be studied. And that... =0A> = =0A>-There is a final sequence =E2=80=9CQuality Control=E2=80=9D that ve= rifies the science =0A>that prpares specimens is properly done so the= tissue can be studied. =0A>And that=E2=80=A6 =0A> =0A>In= order for this all to happen successfully and consistently, the =0A>= HT/HTL's make sure that during the whole preparation process, safety =0A= >is observed, proper adherence to federal and state regulations =0A&g= t;maintained, plus train other technicians to do the same, and much =0A&= gt;more. =0A> =0A>If I understood it all correctly I can=E2=80= =99t help but wonder: =0A> =0A>If HT/HTL's do all of this cruci= al preparation work to make sure =0A>specimens are acceptable for pre= cise microscopic identification of =0A>cells, tissue type, diagnosis = of disease, and other needs: =0A> =0A>"Why wouldn't we want to = have some method that can gage a set of basic =0A>skills to indicate = a level of competency that HT/HTL's should =0A>initially have, in ord= er to enter the field of work that can effect so =0A>many people eith= er directly or indirectly?" =0A> =0A>And also... "Wouldn=E2=80= =99t having NAACLS accredited training and ASCP =0A>Certifications se= rve to gage those basic skills?" =0A> =0A>And also=E2=80=A6 "Wo= uld gaging basic skills have a positive impact on the =0A>quality of = patient care and the efficiency of the HT/HTL=E2=80=99s impact on =0A>= ;labs?=E2=80=9D =0A> =0A>I'm not sure but...this fall=E2=80=A6 = when I complete my NAACLS accredited =0A>degree program, and voluntar= ily take the ASCP HTL certification exam, =0A>my future employer will= be able to expect a certain level of =0A>competency that I hope to h= ave established through training and =0A>certification. =0A> =0A>Rick T. =0A> =0A>______________________________________= _________ =0A>Histonet mailing list =0A>[5]Histonet@lists.utsouthwestern.edu =0A&= gt;[6]ht= tp://lists.utsouthwestern.edu/mailman/listinfo/histonet =0A> = =0A> =0A> =0A> =0A>________________________________ =0A> =0A>From: Rick Tiefenauer <[7]gonavy2003@gmail.com> =0A>To: [8]histonet@lists.utsouthwestern.edu = =0A>Sent: Saturday, May 26, 2012 12:52 PM =0A>Subject: Re: [Histon= et] Unregistered techs =0A> =0A>_______________________________= ________________ =0A>Histonet mailing list =0A>[9]Histonet@lists.utsouthwestern.edu<= br>=0A>[10]http://lists.utsouthwestern.edu/mailman/listinfo/histonet =0A_______________________________________________ =0AHistonet mailing li= st =0A[11]Histonet@list= s.utsouthwestern.edu =0A[12]http://lists.utsouthwestern.edu/mailman/listinfo/= histonet =0A=0A =0A References 1. 3D"mailto:techmana12@yahoo= 2. 3D"mailto:histotalk@yahoo.com" 3. 3D"mailto:Histonet= 4. =3D"mailto:histotalk@yahoo.com" 5. 3D"mailto:Histo= 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 7. 3D"mailto:gonavy2003@g= 8. 3D"mailto:hist= 9. file://localhost/tmp/3D"mailt= 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histo= 11. 3D"mailto:Histonet@lists.utsouthwestern.edu" 12. 3D"http://lists.utsouthwestern.edu/m= From joelleweaver <@t> hotmail.com Tue May 29 12:27:00 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue May 29 12:27:09 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <20120529101829.f86bd30e73b823f57b516b5451216a98.a613e9ad39.wbe@email01.secureserver.net> References: <20120529101829.f86bd30e73b823f57b516b5451216a98.a613e9ad39.wbe@email01.secureserver.net> Message-ID: I thought so too, and that was the case where I am originally from- but no, not really it seems to vary by geographic location. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: pruegg@ihctech.net > To: techmana12@yahoo.com; histotalk@yahoo.com; Histonet@Lists.UTSouthwestern.edu > Date: Tue, 29 May 2012 10:18:29 -0700 > Subject: RE: [Histonet] Unregistered techs > CC: > > > There is nothing volunteer about being ASCP certified as an HT or=L > where I have worked for the last 35 years, all those employed as HT's > = the U of Colorado must be ASCP certified and I believe this is > the case =r most other places doing hospital based Histology, work, > right???? > > > > > > > > > ------= Original Message -------- > > Subject: Re: [Histonet] Unregistered tec= > > From: Dorothy Ragland-Glass <[1]techmana12@yahoo.com> > > Date: Mon, May 28, 2012 10:14 pm > To: David Kemler <[2]histotalk@ yahoo.com>, Fellow HistoNetters > > <[3]Histonet@Lists.UTSouthwestern.edu> > > > Dorothy R. Glass, BS,HTL(ASCP),IHC > > You are so right about =e good old days when you would prove > who you were and sit for a paper not=mputer exam at a medical > school close to you. I sat for the HTL in 1988.=as so proud to > call myself a Histologist after being trained at a Schoo=f > Histotechnology and being ASCP certified. NOW you have people on the > j= trained just referring to themselves casually as histotects. Not > histo t=inees. Unfortunate for us older techs, it is very offensive > considering w=t we went through in establishing a career not just a > job. Some reference=bs is focusing on hiring aids to later, after a > few months train them an=efer to them as histotechs. I hate it > when the term is used so freely. > Sinserely, frustrated HTL > > > > > > David Kemler <[4]histotalk@yahoo.com> wrote: > > > >Good stuff. In the "old" days, 36 years ago for me, taking th e HT(ASCP) exam it was said that you were "registered" by the ASC= > because the designation HT is given by the Board of Registry of the ASCP. Many of the NEW folks use the word "certified". After a total o f 39 years (3 years was training before you were eligible) I=ill > only use registered by the ASCP / Licensed by the State of Florida&n bsp;and call myself a "histologist". > > > > > >In those d=s ( long before the Internet), you took your ASCP > exam (HT's, MT's, CT's,='s, MLT's) at specially selected medical > schools across the US. You=ose the one giving the exam which was > closest to where you lived. If you=eded to drive 100 miles or > further to get to the examinin=ollege on March 15 OR August 15th, > (the only dates it was given)&nb=;that's what you did. Getting into > the exam auditorium before y= were allowed to "sit" (that's what it > was called) for the exam, was?hallenge. You had to prove that you > were who you said you were or you&n=p;were not getting in. Once > those guarded doors were closed - t=y were CLOSED! I saw several > folks crying outside the auditorium tha=ay n 1975. Chances for > cheating were eliminated at every turn. Unfortuna=ly, not so today. > So you can see why for us older techs, i=ou were HT(ASCP) it > really meant something. Unfortunately, not so t=ay. > > > > > >Yours, > > >Dave > > >Hist=etters, > > > > > >I see this subject tends to illicit strong s=timents from > > >professionals who are impacted or have an impact o=T/HTL's > (sort of > > >everyone on the net)?. > > > > > >I= still in school, but I want to fully understand how > training, > > >=ertification, and registration work for HT/HTL?s. I > realize=at > > >ASCP certification is voluntary, and that some States requi= > some > > >sort of license or certification, but I?ve never =ard > of a ?Registry? > > >for HT/HTL?s. > =A> > > >-The way I understand through what I?ve been taug= at > school is that > > >Histology is the study of tissue, And that..= > > > > > >-To study tissue there is another science that prepa=s > specimens so > > >they can be studied. And that... > > > > =A>-There is a final sequence ?Quality Control? > that ve=fies the science > > >that prpares specimens is properly done so the=ssue can be > studied. > > >And that? > > > > > >In=der for this all to happen successfully and consistently, > the > > >=/HTL's make sure that during the whole preparation process, > safety > > =s observed, proper adherence to federal and state regulations > > &g=maintained, plus train other technicians to do the same, and > much > > &=;more. > > > > > >If I understood it all correctly I can?=9t help but > wonder: > > > > > >If HT/HTL's do all of this cruci= preparation work to make sure > > >specimens are acceptable for pre=se microscopic identification > of > > >cells, tissue type, diagnosis = disease, and other needs: > > > > > >"Why wouldn't we want to =ve some method that can gage a set of > basic > > >skills to indicate =evel of competency that HT/HTL's should > > >initially have, in ord= to enter the field of work that can > effect so > > >many people eith= directly or indirectly?" > > > > > >And also... "Wouldn?=9t having NAACLS accredited training > and ASCP > > >Certifications se=e to gage those basic skills?" > > > > > >And also? "Wo=d gaging basic skills have a positive > impact on the > > >quality of =tient care and the efficiency of the > HT/HTL?s impact on > > >=abs?? > > > > > >I'm not sure but...this fall? =en I complete my NAACLS > accredited > > >degree program, and voluntar=y take the ASCP HTL certification > exam, > > >my future employer will? able to expect a certain level of > > >competency that I hope to h=e established through training and > > >certification. > > > > > >Rick T. > > > > > >______________________________________=_______ > > >Histonet mailing list > > >[5]Histonet@lists.utsouthwestern.edu > > &=;[6]ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > =A> > > > > > > > > >________________________________ > > > > > >From: Rick Tiefenauer <[7]gonavy2003@gmail.com> > > >To: [8]histonet@lists.utsouthwestern.edu > =A>Sent: Saturday, May 26, 2012 12:52 PM > > >Subject: Re: [Histon=] Unregistered techs > > > > > >_______________________________=______________ > > >Histonet mailing list > > >[9]Histonet@lists.utsouthwestern.edu< br> > >[10]http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing li= > > [11]Histonet@list=utsouthwestern.edu > > [12]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet > > > > > > > > References > > 1. 3D"mailto:techmana12@yahoo 2. 3D"mailto:histotalk@yahoo.com" > 3. 3D"mailto:Histonet 4. ="mailto:histotalk@yahoo.com" > 5. 3D"mailto:Histo 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > 7. 3D"mailto:gonavy2003@g 8. 3D"mailto:hist 9. file://localhost/tmp/3D"mailt 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histo 11. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 12. 3D"http://lists.utsouthwestern.edu/m_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.thotakura <@t> imperial.ac.uk Tue May 29 12:30:41 2012 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Tue May 29 12:30:57 2012 Subject: [Histonet] IHC on paraffin sections Message-ID: Dear All, I want to stain for inos, Dectin-1 and Hem OX-1 on formalin fixed paraffins sections. I was told by some one these antibodies only work on frozen sections and not on paraffin sections, but I see all these antibodies datasheet say it work on paraffin sections as well. Any input will be helpful. Many Thanks, Kumar. From one_angel_secret <@t> yahoo.com Tue May 29 13:58:43 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue May 29 13:58:49 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <20120529101829.f86bd30e73b823f57b516b5451216a98.a613e9ad39.wbe@email01.secureserver.net> References: <20120529101829.f86bd30e73b823f57b516b5451216a98.a613e9ad39.wbe@email01.secureserver.net> Message-ID: <1338317923.76347.YahooMailNeo@web112320.mail.gq1.yahoo.com> Lets just get to the crux of all this shall we. ? In some states where license rules. It goes like this. ? I'll use Florida because thats where I am. ? Back in the day the state offered their own test. 3 of them to be specific, technician, technologist and general lab. ? The state decided to stop giving test so they went and allowed the ASCP to offer the way to becoming a histotech. ? Now dont get me wrong, I have a great deal of respect for ASCP, although I do feel they lowered the standards when they got rid of the technical test portion. ? I know many histotechs that went through a internet AS degreed program, such as Florida State College. a Great program by the way. They still require students to go to campus occasionally and a long technical practicum somehwere. These students get?QUALITATIVE CHEMISTRY?and microbiology. plus a very intensive practicum.?You used to have to take immunology, hematology, phlebotomy and some other stuff I cant remember. Never the less still one of the best colleges to seek good techs from.?< yes, Im biased. ? But here's what I'm seeing. Many of these out of state programs take in students they NEVER lay an eye on. These people pass a test and they are histotechs. And guess what, many of these students all they care about is passing that test and getting that title and fat paycheck much like all I care about taxes is that Ive filled it out and now send me a return (hopefully). ? I'm going to catch a bunch of stuff for this. But if none of you want to address it, then I have no choice. Because I'm just stuborn that way and I really care about this profession and by god Im getting older and desire for people coming into this profession to?have enough knowledge to solve problems. Why? because patient care really is still driving me after all these years. ? So back to license or requiring ASCP. No, you dont have to have ASCP if youve went though all this other stuff Ive talked about in Florida. You are lisensed. But if you are new, yes you have to get ASCP to get one. ? But even if you go through a online internet program and DO NOT have a higher education, Florida will still only reconize to license you as a technician. You will need to have some years under your belt as a technician to take?the route to become technologist, then more years to become supervisor. ? There is no real OJT in Florida, you would have to be registered with the state and given a temporary license and you would have to show where you are studying in a approved course to get it. ? Who knows, maybe you even had your husband do the program for you while you were at work or making dinner. But you still have to pass that test so maybe you are a good test taker. Who knows. ? Now, if anyone wants to bash me up, go ahead. Because I have had at least 8 people in the last 2 years who couldnt even tell me what stain stains a nucleus who has graduated from a online course. ? If you want respect, raise the bar. If you raise it so high that I have?even half?to jump through more hoops , so be it. But dont just ignore where we ALL know the problem is. We are NOT politicians. ? I care for this place and all of you as professionals and I am sorry for being so blunt because I know this is going to hurt me. ? ? Good Day. ? Kim D ? ? ? ________________________________ From: "pruegg@ihctech.net" To: Dorothy Ragland-Glass ; David Kemler ; Fellow HistoNetters Sent: Tuesday, May 29, 2012 1:18 PM Subject: RE: [Histonet] Unregistered techs ? There is nothing volunteer about being ASCP certified as an HT or= HTL ? where? I have worked for the last 35 years, all those employed as HT's ? =? at? the? U of Colorado must be ASCP certified and I believe this is ? the case = for most other places doing hospital based Histology, work, ? right???? ? ? ? ? ------= -- Original Message -------- ? Subject: Re: [Histonet] Unregistered tec= hs ? From: Dorothy Ragland-Glass <[1]techmana12@yahoo.com> ? Date: Mon, May 28, 2012 10:14 pm To: David Kemler <[2]histotalk@? yahoo.com>, Fellow HistoNetters ? <[3]Histonet@Lists.UTSouthwestern.edu> ? ? Dorothy R. Glass, BS,HTL(ASCP),IHC ? ? You? are? so right about = the good old days when you would prove ? who? you? were? and? sit? for? a paper not= computer exam at a medical ? school? close? to? you.? I sat for the HTL in 1988.= I was so proud to ? call? myself? a? Histologist? after? being? trained? at? a Schoo= l of ? Histotechnology? and? being ASCP certified. NOW you have people on the ? j= ob trained just referring to themselves casually as histotects. Not ? histo t= rainees. Unfortunate for us older techs, it is very offensive ? considering w= hat we went through in establishing a career not just a ? job. Some reference= labs is focusing on hiring aids to later, after a ? few? months? train? them? an= d refer to them as histotechs. I hate it ? when the term is used so freely. Sinserely, frustrated HTL ? ? ? David Kemler <[4]histotalk@yahoo.com> wrote: ? >Good? stuff. In the "old" days, 36 years ago for me, taking th? e? HT(ASCP) exam it was said that you were "registered" by the ASC= P, ? because? the? designation HT is given by the Board of Registry of the? ASCP. Many of the NEW folks use the word "certified". After a total o? f 39? years? (3? years was training before you were eligible) I= still ? only? use registered by the ASCP / Licensed by the State of Florida&n? bsp;and call myself a "histologist". ? > ? >In? those? d=? ays ( long before the Internet), you took your ASCP ? exam? (HT's,? MT's,? CT's,= BT's, MLT's) at specially selected medical ? schools? across? the? US. You= chose the one giving the exam which was ? closest? to? where? you? lived.? If you=? needed to drive 100 miles or ? further to? get to the examinin= g college on March 15 OR August 15th, ? (the only dates it was given)&nb= sp;that's what you did. Getting into ? the exam auditorium before y= ou were allowed to "sit" (that's what it ? was? called) for the exam, was= a challenge. You had to prove that you ? were? who? you? said? you were or you&n= bsp;were not getting in. Once ? those? guarded? doors? were closed - t= hey were CLOSED! I saw several ? folks? crying? outside the? auditorium? tha= t day n 1975. Chances for ? cheating were eliminated at every turn. Unfortuna= tely, not so today. ? So? you can? see? why? for? us older? techs, i= f you were HT(ASCP) it ? really meant something. Unfortunately, not so t= oday. ? > ? >Yours, ? >Dave ? >Hist= onetters, ? > ? >I see this subject tends to illicit strong s= entiments from ? >professionals? who? are? impacted? or have an impact o= n HT/HTL's ? (sort of ? >everyone on the net)?. ? > ? >I=? am? still? in? school,? but? I? want? to? fully understand how ? training, ? >=? ;certification,? and registration work for HT/HTL???s.? I ? realize= that ? >ASCP? certification? is? voluntary, and that some States requi= re ? some ? >sort? of? license or certification, but I???ve never = heard ? of a ???Registry??? ? >for HT/HTL???s. ? = > ? >-The? way? I understand through what I???ve been taug= ht at ? school is that ? >Histology is the study of tissue, And that..= . ? > ? >-To? study? tissue? there? is? another? science? that? prepa=? res ? specimens so ? >they can be studied. And that... ? > ? =? >-There? is? a? final sequence ???Quality Control??? ? that ve= rifies the science ? >that? prpares? specimens? is? properly? done so the= tissue can be ? studied. ? >And that??? ? > ? >In=? order? for? this all to happen successfully and consistently, ? the ? >=? HT/HTL's? make? sure that during the whole preparation process, ? safety ? = >is observed, proper adherence to federal and state regulations ? &g=? t;maintained, plus train other technicians to do the same, and ? much ? &= gt;more. ? > ? >If? I? understood? it? all? correctly? I? can??= ?t help but ? wonder: ? > ? >If HT/HTL's do all of this cruci= al preparation work to make sure ? >specimens? are acceptable for pre= cise microscopic identification ? of ? >cells, tissue type, diagnosis = of disease, and other needs: ? > ? >"Why wouldn't we want to = have some method that can gage a set of ? basic ? >skills to indicate = a level of competency that HT/HTL's should ? >initially? have,? in? ord=? er to enter the field of work that can ? effect so ? >many people eith= er directly or indirectly?" ? > ? >And? also... "Wouldn??= ?t having NAACLS accredited training ? and ASCP ? >Certifications se= rve to gage those basic skills?" ? > ? >And? also???? "Wo=? uld? gaging basic skills have a positive ? impact on the ? >quality? of? =? patient? care? and? the? efficiency? of? the ? HT/HTL???s impact on ? >= ;labs???? ? > ? >I'm? not sure but...this fall??? = when I complete my NAACLS ? accredited ? >degree? program, and voluntar= ily take the ASCP HTL certification ? exam, ? >my future employer will= be able to expect a certain level of ? >competency that I hope to h= ave established through training and ? >certification. ? > ? >Rick T. ? > ? >______________________________________= _________ ? >Histonet mailing list ? >[5]Histonet@lists.utsouthwestern.edu ? &= gt;[6]ht? tp://lists.utsouthwestern.edu/mailman/listinfo/histonet ? > ? = > ? > ? > ? >________________________________ ? > ? >From: Rick Tiefenauer <[7]gonavy2003@gmail.com> ? >To: [8]histonet@lists.utsouthwestern.edu ? = >Sent: Saturday, May 26, 2012 12:52 PM ? >Subject: Re: [Histon= et] Unregistered techs ? > ? >_______________________________= ________________ ? >Histonet mailing list ? >[9]Histonet@lists.utsouthwestern.edu >[10]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ ? Histonet mailing li= st ? [11]Histonet@list= s.utsouthwestern.edu ? [12]http://lists.utsouthwestern.edu/mailman/listinfo/= histonet ? ? ? References ? 1. 3D"mailto:techmana12@yahoo? 2. 3D"mailto:histotalk@yahoo.com" ? 3. 3D"mailto:Histonet? 4. ="mailto:histotalk@yahoo.com" ? 5. 3D"mailto:Histo? 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ? 7. 3D"mailto:gonavy2003@g? 8. 3D"mailto:hist? 9. file://localhost/tmp/3D"mailt? 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histo? 11. 3D"mailto:Histonet@lists.utsouthwestern.edu" ? 12. 3D"http://lists.utsouthwestern.edu/m_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Tue May 29 14:02:00 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Tue May 29 14:02:17 2012 Subject: [Histonet] RE: Not requiring HT Certification) In-Reply-To: <53D6ADCF-AF81-45B4-B416-6E2F7D856C7B@yahoo.com> References: <53D6ADCF-AF81-45B4-B416-6E2F7D856C7B@yahoo.com> Message-ID: <3328693C43A557458850CC37CE16CD1877954A3A@chw-msg-829.chw.edu> I learned 22 years ago with OJT and then went and got certified a year later. I also love to teach. What I am finding now days is the new techs only know how to embed and cut but push buttons for everything else. To me they missed a very important step in their training. Very hard to find one that can actually manually do special stains. I personally like to do mine by hand because I think the quality is so much better. I know this is hard for very large labs. Just my two cents, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, May 25, 2012 7:03 AM To: Mayer,Toysha N Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Not requiring HT Certification) I personally agree with your route. What I am most concerned with is so many want a quick route these days and don't want to put in the hard work to learn the theory. They just want to pass the test, get a job and make a big paycheck and expect the lab that hires them to actually teach them. I enjoy teaching myself to a degree but times are changing and we are expected to do more with less. We need graduating techs to be hit the ground ready day one. If we don't start to expect this you can count on with all the cut backs in health care that our profession will continue to be viewed as just a bunch of monkeys and therefore will be payed as a bunch of monkeys. And personally being refered to as a monkey ticks me off. I'm not a monkey. I'm a frog lol Sent from my iPhone On May 25, 2012, at 9:25 AM, "Mayer,Toysha N" wrote: > > > Like everyone else, I was going to keep quiet, but I can't. > I am sensitive to those downgrading us who took the OJT route. > I did the OJT route, had a BS in Biology, tried for Veterinary School, but that wasn't so. What do I do with all of this Science? As part of my Pre-Med curricula had to take histology, loved it. Had no idea that it was a paying field and such. Had I known, I would have applied to a school as a backup. It took me a while, but when I was hired as a tech, I did not excel quickly (Cheryl remember my mistakes), but I learned and had patient coworkers. > Move on down the line several years and another coworker questioned my knowledge of the chemistry behind a stain, and all of a sudden all of my organic and biochem can running out of my mouth. I didn't realize that I really knew all of that. I had always felt a little disadvantaged because I did not get formal training through a school, just studied and passed the HT with the help of my coworkers (shout out to LSU VetPath). > There is nothing wrong with OJT for Biology majors. They should have the basic background to understand the chemistries and processes behind why we do what we do. With the modernization of technologies and procedures in the histo lab some formal education is needed. There are many ways to receive this education, it can be online, or in person. It all depends on the learner. > To overcome the stigma we should continue with some of the things that are now in place to stabilize the training of our successors (yes we all are going to have to retire one day). A continued push for formal training, promotion of the field, professionalism by our colleagues, and respect from the customers (pathologists, patients, and gen lab personnel). > In order to facilitate change for respect, we must first present a unified front. > I know good techs with no certification, I know bad techs with certification. We all do. > Last year I celebrated my 20th year in histo and never thought I would be where I am. I never wanted to do research, and didn't like management (don't like telling grown folks what to do), but I love teaching. It helps me to learn the theory behind what I do and apply it better. There are so many people who can do histo whether it is routine, special procedures, or management. > We shouldn't look down on those who took the OJT route, sometimes they just don't know about a formal program (like me). You never know where the next great manager, director or tech is coming from, so don't count them out. > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 24 May 2012 17:20:41 +0000 > From: joelle weaver > Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Jon There is a route with associates and training I believe. > Of course I can't speak for the BOC, and I am sure that you want to help your employees as much as you can. I do see your point about the similarities in tasks. My thought would be that the exam eligibility states that they have to have recent experience in fixation, embedding, microtomy, and staining (histology) and the associated theory knowledge. EM is on the exam study topics, but also with the theory/experience for all those routine histological techniques, is how I read it. Take a look at the exam outlines, that should give you an idea of the scope. Ascp.org "get certified". As I have been told, they want to cover the widest possible scope of roles histologists can perform, which could include EM, but not only that. If they don't have exposure to regular histology I think that it might be hard for to feel prepared for the regular HT or HTL exams. That's just my opinion, based on what I have observed and also the pass rates ( ~ 65%), for people even with training/experience- there could be an exceptional person out there. I can understand not wanting to get buried in doing a whole HT curricula ( believe me, I do). How about the option of having cross training in a histology lab? Do you have routine histology on site or a nearby lab? The best advice I can give is to go to the website and carefully read the requirments to see how your employees might fit in. If you want to provide the theory without having to do the curricula, there are on line programs out there which can supplement OJT and a supportive mentor and organization. I have seen this work successfully with motivated people with the ability to have hands on practice alongside. I suggest the NSH site which lists the accredited programs or the NAACLS site which has a search for programs, if that would help. As far as employability, my opinion is that it would certainly open up options for your employees to also have skills in routine histology make them more valuable to your organization, and I would think certification would be even more helpful to them as far as options. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) >> From: jkrupp@deltacollege.edu >> Date: Thu, 24 May 2012 09:53:21 -0700 >> CC: histonet@lists.utsouthwestern.edu >> To: joelleweaver@hotmail.com >> >> >> On May 24, 2012, at 9:25 AM, joelle weaver wrote: >> >>> >>> I think that the original post was referring to people with a HSD or GED. I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession. The people with bachelors and the science credits needed can choose either the HT or HTL as I understand it, with the one year of verified experience/training. I think there is a route with a associate's and the required science credits available with the verified training for the HT. I believe there are now two routes for each exam if I recall correctly, one being the program graduate route plus experience , and one with education requirements met and training/experience. There is a page on the site that lays this out if anyone wants to clarify/correct my recollection. >> >> OK, so I have a question. >> >> We train students to do electron microscopy, both specimen prep and instrument operation. They fix and embed (in plastic) tissues and make thick sections (1 um or less for us) and examine them using LM. It is not much of a leap to add paraffin techniques and/or basic staining etc. We have most of the equipment that would be needed already, but I am not ready to go into a full blown HT curriculum. >> >> So, the question is, if a student gets an Associates degree that includes the basic science, would it help the student to get the basics of HT before looking for a job? Or could they take the test, get something to show for their work and make them a good job candidate? >> >> Our students are skilled and could do the job, but figuring out how to help them and give them the right advice is my problem now. >> >> Jon >> >> Jonathan Krupp >> Delta College >> 5151 Pacific Ave. >> Box 212 >> Stockton, CA 95207 >> 209-954-5284 >> jkrupp@deltacollege.edu >> >> Find us on Facebook @ >> Electron Microscopy at SJ Delta College >> >> >> >> >> >> > > > ------------------------------ > > Message: 2 > Date: Thu, 24 May 2012 17:28:10 +0000 > From: joelle weaver > Subject: RE: [Histonet] RE: Not requiring HT Certification > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Yes I was referring to the HS and OJT training route. There is confusion about the degree + OJT and HS + OJT. Yes, that was my understanding also of the drivers, I just could not recall where I read/heard that. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: Timothy.Morken@ucsfmedctr.org >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 24 May 2012 09:56:25 -0700 >> Subject: [Histonet] RE: Not requiring HT Certification >> >> " I am not sure of the exact reasons considered in discontinuation of OJT route, but I recall some publications discussing the desire to raise the perception and awareness of the profession." >> >> Just a clarification, the OJT route was NOT discontinued - it is still there - even now the vast majority of techs are trained by OJT - maybe 99.9 percent. They simply need more education (not even a degree, just enough credits!) to qualify to take the HT test. >> >> If you mean the pure OJT route - no education beyond high school, well, the primary driver was the fact that almost all techs could pass the practical but the pass rate on the written test was much, much lower. It became obvious that many people were doing lab work that they did not fully understand or had trouble comprehending the details. >> >> >> Tim Morken >> >> > ******************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue May 29 14:09:35 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 29 14:10:10 2012 Subject: [Histonet] Lab Office position References: <4FC4E3BA020000770003953A@gwmail3.harthosp.org> <4FC4E6AF0200007700039541@gwmail3.harthosp.org> Message-ID: <4FC4E6AF.7400.0077.0@harthosp.org> I know someone here in Connecticut who is thinking about re-locating to the Melbourne/Palm Bay area of Florida. She currently works in a busy Anatomic Pathology office in a hospital doing specimen accessioning, consult accessioning, slide(s) send-outs, slide filing, etc. Are there any jobs in this part of FL for a person with this skill-set? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Sandra.Harrison3 <@t> va.gov Tue May 29 15:12:07 2012 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue May 29 15:12:49 2012 Subject: [Histonet] compatibility of Thermo slide printers with Leica cassette printer Message-ID: 1) Does anyone have a Leica Cassette Printer on which they are printing 2-Dimensional barcodes? 2) Is anyone using a Thermo slide printer, with bar code reader, to read cassettes printed off of a Leica Cassette Printer with 2-D bar codes? Thanks for your input. Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From tkngflght <@t> yahoo.com Tue May 29 15:58:20 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue May 29 15:58:24 2012 Subject: [Histonet] floaters - thisisann@aol.com Message-ID: <1338325100.66322.YahooMailNeo@web39402.mail.mud.yahoo.com> Hi Ann- ? May we assume you've confirmed this is happening at embedding and?have ruled?out?any floaters happening during cutting? ? When embedding, keep Kimwipes or other tissues around, keep the wells closed and only pull out enough cassettes that you can keeep clean and clear of your working area.? Wipe forceps and surfaces?between blocks containing fragmented or friable tissue, don't put forceps back in the wells without wiping. You can stack a few guaze pads on top of the spout to wipe as you replace the forceps and change the pads frequently.???Always, always only open one cassettte at a time and never leave the station with an open cassette on the station.? Finish before standing or recap and replace.? ? If you are working with currettes, cell blocks,?or other cellular, friable tissue, open and unwrap?on a clean surface (hot or cold - embedding station surface or wipe or l'absorb) and don't reuse the surface before wiping or replacing.? If you're using knives or scalpels to scrape, make sure the handles and connection points aren't harboring residual tissue. Buy those little seamless paring knive from the dollar store--they fit in the wells and wipe easily.? Use swabs between embedding sessions or between people trading places?to clean the wells and then clean them again at the end of the embedding session.? ? If you keep molten paraffin in the hold bins, filter or replace frequently and do not reuse.? If you keep the hold bins dry, clean routinely (daily)? Clean your molten wax chamber periodically to remove contaminants and keep the filter from clogging over time. Most embedding station mfc don't condone running xylene through the tubes & pumps--clean hot wax will do the job. ? We always make it the responsibility of each person to clean both at the end of embedding AND to clean again before starting to fully assure the wells and surfaces were clear and eliminate a possible cross if one person in the chain forgets...double system processes like you double check specimen IDs. ? You'll go through a whole bunch of kimwipes--but they are much cheaper than gauze and SOOO much better than a cross contamination situation.? IF it still happens, it's time to track who embeds each block?to see if there's a pattern by person.? The point is not to write people?up but?to support developing clean habits and to adjust their habits to do it to their best ability.? ? Wipe wipe wipe wipe wipe!!? Hope this helps! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From afimbres <@t> uci.edu Tue May 29 19:08:08 2012 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Tue May 29 19:08:15 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: Message-ID: I hope I've misunderstood but I want to clarify that the ASCP computer exam for technicians and technologists is NOT inferior to those that "sat" for an exam at their local medical school. "Chances for cheating were eliminated at every turn." This still holds true for the ASCP computer exam as well. I'm not sure if you're familiar with the process, but one has to bring a state issued picture ID (or equivalent) which has to match the name exactly to the exam application (no ifs, ands, or buts). Then, they ask you to turn out all your pockets and if you have anything on you (including keys) it goes into a locker. Then they fingerprint you and tell you that as you are taking the exam, you will be videotaped AND recorded while you sit there. If you leave to use the restroom, that whole procedure is repeated. Please do not assume that sitting for the exam back in the day is somehow superior to those that take the computer exams nowadays. We ALL worked hard studying for those exams and we should be united in our stance that HT/HTL certification is important to all of us. Stepping off the soap box now, Amber M. Fimbres, MHA CT(ASCP)HTL Proudly took her HT, HTL, and CT using the ASCP BOR computer exam -----Original Message----- From: Dorothy Ragland-Glass [mailto:techmana12@yahoo.com] Sent: Monday, May 28, 2012 10:15 PM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] Unregistered techs Dorothy R. Glass, BS,HTL(ASCP),IHC You are so right about the good old days when you would prove who you were and sit for a paper not computer exam at a medical school close to you. I sat for the HTL in 1988. I was so proud to call myself a Histologist after being trained at a School of Histotechnology and being ASCP certified. NOW you have people on the job trained just referring to themselves casually as histotects. Not histo trainees. Unfortunate for us older techs, it is very offensive considering what we went through in establishing a career not just a job. Some reference labs is focusing on hiring aids to later, after a few months train them and refer to them as histotechs. I hate it when the term is used so freely. Sinserely, frustrated HTL David Kemler wrote: >Good stuff. In the "old" days, 36 years ago for me, taking the HT(ASCP) exam it was said that you were "registered" by the ASCP, because the designation HT is given by the Board of Registry of the ASCP. Many of the NEW folks use the word "certified". After a total of 39 years (3 years was training before you were eligible) I still only use registered by the ASCP / Licensed by the State of Florida and call myself a "histologist". > >In those days ( long before the Internet), you took your ASCP exam >(HT's, MT's, CT's, BT's, MLT's) at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you needed to drive 100 miles or further to get to the examining college on March 15 OR August 15th, (the only dates it was given) that's what you did. Getting into the exam auditorium before you were allowed to "sit" (that's what it was called) for the exam, was a challenge. You had to prove that you were who you said you were or you were not getting in. Once those guarded doors were closed - they were CLOSED! I saw several folks crying outside the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately, not so today. So you can see why for us older techs, if you were HT(ASCP) it really meant something. Unfortunately, not so today. > >Yours, >Dave >Histonetters, > >I see this subject tends to illicit strong sentiments from >professionals who are impacted or have an impact on HT/HTL's (sort of >everyone on the net)?. > >I am still in school, but I want to fully understand how training, >certification, and registration work for HT/HTL's. I realize that ASCP >certification is voluntary, and that some States require some sort of >license or certification, but I've never heard of a "Registry" >for HT/HTL's. > >-The way I understand through what I've been taught at school is that >Histology is the study of tissue, And that... > >-To study tissue there is another science that prepares specimens so >they can be studied. And that... > >-There is a final sequence "Quality Control" that verifies the science >that prpares specimens is properly done so the tissue can be studied. >And that... > >In order for this all to happen successfully and consistently, the >HT/HTL's make sure that during the whole preparation process, safety is >observed, proper adherence to federal and state regulations maintained, >plus train other technicians to do the same, and much more. > >If I understood it all correctly I can't help but wonder: > >If HT/HTL's do all of this crucial preparation work to make sure >specimens are acceptable for precise microscopic identification of >cells, tissue type, diagnosis of disease, and other needs: > >"Why wouldn't we want to have some method that can gage a set of basic >skills to indicate a level of competency that HT/HTL's should initially >have, in order to enter the field of work that can effect so many >people either directly or indirectly?" > >And also... "Wouldn't having NAACLS accredited training and ASCP >Certifications serve to gage those basic skills?" > >And also... "Would gaging basic skills have a positive impact on the >quality of patient care and the efficiency of the HT/HTL's impact on >labs?" > >I'm not sure but...this fall... when I complete my NAACLS accredited >degree program, and voluntarily take the ASCP HTL certification exam, >my future employer will be able to expect a certain level of competency >that I hope to have established through training and certification. > >Rick T. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >________________________________ > >From: Rick Tiefenauer >To: histonet@lists.utsouthwestern.edu >Sent: Saturday, May 26, 2012 12:52 PM >Subject: Re: [Histonet] Unregistered techs > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From tracykme <@t> comcast.net Tue May 29 20:07:04 2012 From: tracykme <@t> comcast.net (Tracy) Date: Tue May 29 20:07:25 2012 Subject: [Histonet] Please unsubscribe Message-ID: From pruegg <@t> ihctech.net Tue May 29 21:17:45 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue May 29 21:17:53 2012 Subject: [Histonet] Unregistered techs In-Reply-To: <1338317923.76347.YahooMailNeo@web112320.mail.gq1.yahoo.com> References: <20120529101829.f86bd30e73b823f57b516b5451216a98.a613e9ad39.wbe@email01.secureserver.net> <1338317923.76347.YahooMailNeo@web112320.mail.gq1.yahoo.com> Message-ID: <1FF4581352C14EB18984F1EA67856FB6@prueggihctechlt> I am sorry but I must be living in dream world, in the last 9 years I have trained at least 4 people in histology who passed the Ht or the HTL exam and I would put them up against any histotech for knowing what stains the nucleus, special stains, as well as being really well trained (not just to chose the right bar code) in IHC, to do IHC manually and trouble shoot problems that do go wrong with autostainers . in my community ASCP certification and even more than that who trained you and how you were trained matters. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _____ From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Tuesday, May 29, 2012 12:59 PM To: pruegg@ihctech.net; Dorothy Ragland-Glass; David Kemler; Fellow HistoNetters Subject: Re: [Histonet] Unregistered techs Lets just get to the crux of all this shall we. In some states where license rules. It goes like this. I'll use Florida because thats where I am. Back in the day the state offered their own test. 3 of them to be specific, technician, technologist and general lab. The state decided to stop giving test so they went and allowed the ASCP to offer the way to becoming a histotech. Now dont get me wrong, I have a great deal of respect for ASCP, although I do feel they lowered the standards when they got rid of the technical test portion. I know many histotechs that went through a internet AS degreed program, such as Florida State College. a Great program by the way. They still require students to go to campus occasionally and a long technical practicum somehwere. These students get QUALITATIVE CHEMISTRY and microbiology. plus a very intensive practicum. You used to have to take immunology, hematology, phlebotomy and some other stuff I cant remember. Never the less still one of the best colleges to seek good techs from. < yes, Im biased. But here's what I'm seeing. Many of these out of state programs take in students they NEVER lay an eye on. These people pass a test and they are histotechs. And guess what, many of these students all they care about is passing that test and getting that title and fat paycheck much like all I care about taxes is that Ive filled it out and now send me a return (hopefully). I'm going to catch a bunch of stuff for this. But if none of you want to address it, then I have no choice. Because I'm just stuborn that way and I really care about this profession and by god Im getting older and desire for people coming into this profession to have enough knowledge to solve problems. Why? because patient care really is still driving me after all these years. So back to license or requiring ASCP. No, you dont have to have ASCP if youve went though all this other stuff Ive talked about in Florida. You are lisensed. But if you are new, yes you have to get ASCP to get one. But even if you go through a online internet program and DO NOT have a higher education, Florida will still only reconize to license you as a technician. You will need to have some years under your belt as a technician to take the route to become technologist, then more years to become supervisor. There is no real OJT in Florida, you would have to be registered with the state and given a temporary license and you would have to show where you are studying in a approved course to get it. Who knows, maybe you even had your husband do the program for you while you were at work or making dinner. But you still have to pass that test so maybe you are a good test taker. Who knows. Now, if anyone wants to bash me up, go ahead. Because I have had at least 8 people in the last 2 years who couldnt even tell me what stain stains a nucleus who has graduated from a online course. If you want respect, raise the bar. If you raise it so high that I have even half to jump through more hoops , so be it. But dont just ignore where we ALL know the problem is. We are NOT politicians. I care for this place and all of you as professionals and I am sorry for being so blunt because I know this is going to hurt me. Good Day. Kim D From: "pruegg@ihctech.net" To: Dorothy Ragland-Glass ; David Kemler ; Fellow HistoNetters Sent: Tuesday, May 29, 2012 1:18 PM Subject: RE: [Histonet] Unregistered techs There is nothing volunteer about being ASCP certified as an HT or= HTL where I have worked for the last 35 years, all those employed as HT's = at the U of Colorado must be ASCP certified and I believe this is the case = for most other places doing hospital based Histology, work, right???? ------= -- Original Message -------- Subject: Re: [Histonet] Unregistered tec= hs From: Dorothy Ragland-Glass <[1]techmana12@yahoo.com> Date: Mon, May 28, 2012 10:14 pm To: David Kemler <[2]histotalk@ yahoo.com>, Fellow HistoNetters <[3]Histonet@Lists.UTSouthwestern.edu> Dorothy R. Glass, BS,HTL(ASCP),IHC You are so right about = the good old days when you would prove who you were and sit for a paper not= computer exam at a medical school close to you. I sat for the HTL in 1988.= I was so proud to call myself a Histologist after being trained at a Schoo= l of Histotechnology and being ASCP certified. NOW you have people on the j= ob trained just referring to themselves casually as histotects. Not histo t= rainees. Unfortunate for us older techs, it is very offensive considering w= hat we went through in establishing a career not just a job. Some reference= labs is focusing on hiring aids to later, after a few months train them an= d refer to them as histotechs. I hate it when the term is used so freely. Sinserely, frustrated HTL David Kemler <[4]histotalk@yahoo.com> wrote: >Good stuff. In the "old" days, 36 years ago for me, taking th e HT(ASCP) exam it was said that you were "registered" by the ASC= P, because the designation HT is given by the Board of Registry of the ASCP. Many of the NEW folks use the word "certified". After a total o f 39 years (3 years was training before you were eligible) I= still only use registered by the ASCP / Licensed by the State of Florida&n bsp;and call myself a "histologist". > >In those d= ays ( long before the Internet), you took your ASCP exam (HT's, MT's, CT's,= BT's, MLT's) at specially selected medical schools across the US. You= chose the one giving the exam which was closest to where you lived. If you= needed to drive 100 miles or further to get to the examinin= g college on March 15 OR August 15th, (the only dates it was given)&nb= sp;that's what you did. Getting into the exam auditorium before y= ou were allowed to "sit" (that's what it was called) for the exam, was= a challenge. You had to prove that you were who you said you were or you&n= bsp;were not getting in. Once those guarded doors were closed - t= hey were CLOSED! I saw several folks crying outside the auditorium tha= t day n 1975. Chances for cheating were eliminated at every turn. Unfortuna= tely, not so today. So you can see why for us older techs, i= f you were HT(ASCP) it really meant something. Unfortunately, not so t= oday. > >Yours, >Dave >Hist= onetters, > >I see this subject tends to illicit strong s= entiments from >professionals who are impacted or have an impact o= n HT/HTL's (sort of >everyone on the net)?. > >I= am still in school, but I want to fully understand how training, >= ;certification, and registration work for HT/HTL???s. I realize= that >ASCP certification is voluntary, and that some States requi= re some >sort of license or certification, but I???ve never = heard of a ???Registry??? >for HT/HTL???s. = > >-The way I understand through what I???ve been taug= ht at school is that >Histology is the study of tissue, And that..= . > >-To study tissue there is another science that prepa= res specimens so >they can be studied. And that... > = >-There is a final sequence ???Quality Control??? that ve= rifies the science >that prpares specimens is properly done so the= tissue can be studied. >And that??? > >In= order for this all to happen successfully and consistently, the >= HT/HTL's make sure that during the whole preparation process, safety = >is observed, proper adherence to federal and state regulations &g= t;maintained, plus train other technicians to do the same, and much &= gt;more. > >If I understood it all correctly I can??= ?t help but wonder: > >If HT/HTL's do all of this cruci= al preparation work to make sure >specimens are acceptable for pre= cise microscopic identification of >cells, tissue type, diagnosis = of disease, and other needs: > >"Why wouldn't we want to = have some method that can gage a set of basic >skills to indicate = a level of competency that HT/HTL's should >initially have, in ord= er to enter the field of work that can effect so >many people eith= er directly or indirectly?" > >And also... "Wouldn??= ?t having NAACLS accredited training and ASCP >Certifications se= rve to gage those basic skills?" > >And also??? "Wo= uld gaging basic skills have a positive impact on the >quality of = patient care and the efficiency of the HT/HTL???s impact on >= ;labs???? > >I'm not sure but...this fall??? = when I complete my NAACLS accredited >degree program, and voluntar= ily take the ASCP HTL certification exam, >my future employer will= be able to expect a certain level of >competency that I hope to h= ave established through training and >certification. > >Rick T. > >______________________________________= _________ >Histonet mailing list >[5]Histonet@lists.utsouthwestern.edu &= gt;[6]ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet > = > > > >________________________________ > >From: Rick Tiefenauer <[7]gonavy2003@gmail.com> >To: [8]histonet@lists.utsouthwestern.edu = >Sent: Saturday, May 26, 2012 12:52 PM >Subject: Re: [Histon= et] Unregistered techs > >_______________________________= ________________ >Histonet mailing list >[9]Histonet@lists.utsouthwestern.edu< br> >[10]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing li= st [11]Histonet@list= s.utsouthwestern.edu [12]http://lists.utsouthwestern.edu/mailman/listinfo/= histonet References 1. 3D"mailto:techmana12@yahoo 2. 3D"mailto:histotalk@yahoo.com" 3. 3D"mailto:Histonet 4. ="mailto:histotalk@yahoo.com" 5. 3D"mailto:Histo 6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 7. 3D"mailto:gonavy2003@g 8. 3D"mailto:hist 9. file://localhost/tmp/3D"mailt 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histo 11. 3D"mailto:Histonet@lists.utsouthwestern.edu" 12. 3D"http://lists.utsouthwestern.edu/m_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From info <@t> morphisto.de Wed May 30 01:14:29 2012 From: info <@t> morphisto.de (Morphisto GmbH) Date: Wed May 30 01:15:02 2012 Subject: [Histonet] compatibility of Thermo slide printers with Leica cassette printer In-Reply-To: References: Message-ID: Hello Sandy, we use a Leica Cassette and Slide Printer which are both connected to a database in which we organize all our specimens and print cassettes and slides, so it is not necessary to read the barcodes from cassettes to print them on the slides. However, there is of course not much space for barcode on the cassettes, but in most cases we can read the barcode with our smart phones. I do not know if the barcodes of Leica and Thermo are compatible. I think you have to use the same type of barcode in both machines, so that the other one can read it. Best regards Michael Am 29.05.2012 um 22:12 schrieb Harrison, Sandra C.: > 1) Does anyone have a Leica Cassette Printer on which they are > printing 2-Dimensional barcodes? > > > > 2) Is anyone using a Thermo slide printer, with bar code reader, to > read cassettes printed off of a Leica Cassette Printer with 2-D bar > codes? > > > > Thanks for your input. > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 60 Telefax: 069 / 989 72 1100 E-Mail: info@morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From one_angel_secret <@t> yahoo.com Wed May 30 05:51:07 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed May 30 05:51:45 2012 Subject: [Histonet] Unregistered techs In-Reply-To: References: Message-ID: <0B9DFF6B-3BDF-41A7-8797-345945A9FFE7@yahoo.com> You've misunderstood. No one has said anything about test at a school. At least I haven't My response at least was referring to those who sat for state exams and then a comparison to the now ASCP exam route. There's a huge difference in what older techs got than what newer ones are getting and anyone who says different. Well I just don't buy it This whole conversation was about why are we not raising the bar. My point was the way it is now has not raised the bar. There is no way to address this topic here without offending some. Which was never my intentions. I tried my best to explain the routes techs take these days compared to the routes way back. And if everyone here thinks that having a bunch of Internet programs where you don't have the other courses and no face time and the focus is to pass a exam then don't get upset. When respect isn't given. I think I've said more than my fairshare on this. I'll just spend the rest of my day pulling the knives from my back. Good luck to all. Sent from my iPhone On May 29, 2012, at 8:08 PM, "Fimbres, Amber" wrote: > I hope I've misunderstood but I want to clarify that the ASCP computer exam for technicians and technologists is NOT inferior to those that "sat" for an exam at their local medical school. > > "Chances for cheating were eliminated at every turn." This still holds true for the ASCP computer exam as well. I'm not sure if you're familiar with the process, but one has to bring a state issued picture ID (or equivalent) which has to match the name exactly to the exam application (no ifs, ands, or buts). Then, they ask you to turn out all your pockets and if you have anything on you (including keys) it goes into a locker. Then they fingerprint you and tell you that as you are taking the exam, you will be videotaped AND recorded while you sit there. If you leave to use the restroom, that whole procedure is repeated. > > Please do not assume that sitting for the exam back in the day is somehow superior to those that take the computer exams nowadays. We ALL worked hard studying for those exams and we should be united in our stance that HT/HTL certification is important to all of us. > > Stepping off the soap box now, > > Amber M. Fimbres, MHA CT(ASCP)HTL > Proudly took her HT, HTL, and CT using the ASCP BOR computer exam > > > > -----Original Message----- > From: Dorothy Ragland-Glass [mailto:techmana12@yahoo.com] > Sent: Monday, May 28, 2012 10:15 PM > To: David Kemler; Fellow HistoNetters > Subject: Re: [Histonet] Unregistered techs > > Dorothy R. Glass, BS,HTL(ASCP),IHC > You are so right about the good old days when you would prove who you were and sit for a paper not computer exam at a medical school close to you. I sat for the HTL in 1988. I was so proud to call myself a Histologist after being trained at a School of Histotechnology and being ASCP certified. NOW you have people on the job trained just referring to themselves casually as histotects. Not histo trainees. Unfortunate for us older techs, it is very offensive considering what we went through in establishing a career not just a job. Some reference labs is focusing on hiring aids to later, after a few months train them and refer to them as histotechs. I hate it when the term is used so freely. > Sinserely, frustrated HTL > > > David Kemler wrote: > >> Good stuff. In the "old" days, 36 years ago for me, taking the HT(ASCP) exam it was said that you were "registered" by the ASCP, because the designation HT is given by the Board of Registry of the ASCP. Many of the NEW folks use the word "certified". After a total of 39 years (3 years was training before you were eligible) I still only use registered by the ASCP / Licensed by the State of Florida and call myself a "histologist". >> >> In those days ( long before the Internet), you took your ASCP exam >> (HT's, MT's, CT's, BT's, MLT's) at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you needed to drive 100 miles or further to get to the examining college on March 15 OR August 15th, (the only dates it was given) that's what you did. Getting into the exam auditorium before you were allowed to "sit" (that's what it was called) for the exam, was a challenge. You had to prove that you were who you said you were or you were not getting in. Once those guarded doors were closed - they were CLOSED! I saw several folks crying outside the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately, not so today. So you can see why for us older techs, if you were HT(ASCP) it really meant something. Unfortunately, not so today. >> >> Yours, >> Dave >> Histonetters, >> >> I see this subject tends to illicit strong sentiments from >> professionals who are impacted or have an impact on HT/HTL's (sort of >> everyone on the net)?. >> >> I am still in school, but I want to fully understand how training, >> certification, and registration work for HT/HTL's. I realize that ASCP >> certification is voluntary, and that some States require some sort of >> license or certification, but I've never heard of a "Registry" >> for HT/HTL's. >> >> -The way I understand through what I've been taught at school is that >> Histology is the study of tissue, And that... >> >> -To study tissue there is another science that prepares specimens so >> they can be studied. And that... >> >> -There is a final sequence "Quality Control" that verifies the science >> that prpares specimens is properly done so the tissue can be studied. >> And that... >> >> In order for this all to happen successfully and consistently, the >> HT/HTL's make sure that during the whole preparation process, safety is >> observed, proper adherence to federal and state regulations maintained, >> plus train other technicians to do the same, and much more. >> >> If I understood it all correctly I can't help but wonder: >> >> If HT/HTL's do all of this crucial preparation work to make sure >> specimens are acceptable for precise microscopic identification of >> cells, tissue type, diagnosis of disease, and other needs: >> >> "Why wouldn't we want to have some method that can gage a set of basic >> skills to indicate a level of competency that HT/HTL's should initially >> have, in order to enter the field of work that can effect so many >> people either directly or indirectly?" >> >> And also... "Wouldn't having NAACLS accredited training and ASCP >> Certifications serve to gage those basic skills?" >> >> And also... "Would gaging basic skills have a positive impact on the >> quality of patient care and the efficiency of the HT/HTL's impact on >> labs?" >> >> I'm not sure but...this fall... when I complete my NAACLS accredited >> degree program, and voluntarily take the ASCP HTL certification exam, >> my future employer will be able to expect a certain level of competency >> that I hope to have established through training and certification. >> >> Rick T. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> ________________________________ >> >> From: Rick Tiefenauer >> To: histonet@lists.utsouthwestern.edu >> Sent: Saturday, May 26, 2012 12:52 PM >> Subject: Re: [Histonet] Unregistered techs >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SStephenson <@t> lifecell.com Wed May 30 06:32:10 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Wed May 30 06:32:18 2012 Subject: [Histonet] Embedding In-Reply-To: References: Message-ID: That was my initial thought, check your paraffin. I could be coming from the paraffin...... or whatever you are cleaning with make sure its dust free/debris free. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax 908.947.1085 Direct: 908.947.1624 ?sstephenson@lifecell.com www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Sunday, May 27, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Hi, Any chance it is from your reservoir? Sometimes the paraffin is not filtered very well and there is some junk that collects in the chamber. (Yes I'm looking at you Paraplast!) Also is the reservoir you keep your cassettes in prior to embedding clean? Perhaps junk on your tampers? Amos On Sat, May 26, 2012 at 1:00 PM, wrote: > Message: 1 > Date: Fri, 25 May 2012 13:02:29 -0400 (EDT) > From: Ann Specian > Subject: [Histonet] Embedding > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CF08AF4CF811BC-C74-9030@webmail-m025.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > We are having a problem with floaters in our blocks which occur during > embedding. We have multiple forceps which are placed in heated wells and > each cassette is embedded with a new forcep. We also wipe with a gauze, > but we are still getting floaters embedded in the cassette from time to > time. > > Does anyone do anything else to prevent this? > Thank you, Ann > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From enarvaez <@t> cblpath.com Wed May 30 06:35:41 2012 From: enarvaez <@t> cblpath.com (Edison Narvaez) Date: Wed May 30 06:35:49 2012 Subject: [Histonet] Please unsuscribe Message-ID: Please unsuscribe From SStephenson <@t> lifecell.com Wed May 30 06:36:23 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Wed May 30 06:36:31 2012 Subject: [Histonet] Leitz 1512 In-Reply-To: References: Message-ID: Yeah, a brand new Leica RM 2255. :D Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax 908.947.1085 Direct: 908.947.1624 ?sstephenson@lifecell.com www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Smallwood Sent: Sunday, May 27, 2012 4:46 PM To: Histonet Submissions Subject: [Histonet] Leitz 1512 Does anyone have a resource for parts for this 40 year old microtome ?? Thanks John Smallwood _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed May 30 08:23:05 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed May 30 08:23:17 2012 Subject: [Histonet] compatibility of Thermo slide printers with Leica cassette printer In-Reply-To: References: Message-ID: We use both the Printmate and slidemate from Thermo. We also have used the Leica IPS for slides. The biggest thing to remember is when using these items is if you are passing them through your APLIS or the printers own proprietary software. Either was you are going to have to look at this with a critical eye. I would suggest using 2D barcodes for both items. 1D tend to increase as the data increases. But this hold true for all barcodes you place on either a slide or cassette. The main thing is the symobology you need in order to produce the barcode. There are several symoblogies out there to use. We currently use the Datamatrix one. This is all driven by either the APLIS or the database you are using. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morphisto GmbH Sent: Tuesday, May 29, 2012 11:14 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] compatibility of Thermo slide printers with Leica cassette printer Hello Sandy, we use a Leica Cassette and Slide Printer which are both connected to a database in which we organize all our specimens and print cassettes and slides, so it is not necessary to read the barcodes from cassettes to print them on the slides. However, there is of course not much space for barcode on the cassettes, but in most cases we can read the barcode with our smart phones. I do not know if the barcodes of Leica and Thermo are compatible. I think you have to use the same type of barcode in both machines, so that the other one can read it. Best regards Michael Am 29.05.2012 um 22:12 schrieb Harrison, Sandra C.: > 1) Does anyone have a Leica Cassette Printer on which they are > printing 2-Dimensional barcodes? > > > > 2) Is anyone using a Thermo slide printer, with bar code reader, to > read cassettes printed off of a Leica Cassette Printer with 2-D bar > codes? > > > > Thanks for your input. > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 60 Telefax: 069 / 989 72 1100 E-Mail: info@morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From b-frederick <@t> northwestern.edu Wed May 30 09:16:03 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed May 30 09:16:13 2012 Subject: [Histonet] CAP Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> They're here!!!!!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From JEllin <@t> yumaregional.org Wed May 30 09:33:43 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed May 30 09:33:49 2012 Subject: [Histonet] RE: CAP In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> Message-ID: Have fun and good luck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, May 30, 2012 7:16 AM To: Fellow HistoNetters Subject: [Histonet] CAP They're here!!!!!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From raestask <@t> grics.net Wed May 30 09:35:43 2012 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Wed May 30 09:35:56 2012 Subject: [Histonet] QIHC Message-ID: <000f01cd3e71$800bdca0$802395e0$@grics.net> Is there any other resource for information and studying for the QIHC exam other than the ASCP outline? Rae Staskiewicz From tajibade <@t> echd.org Wed May 30 09:36:29 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Wed May 30 09:36:41 2012 Subject: [Histonet] RE: CAP In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> Message-ID: Best of luck. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel: 432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, May 30, 2012 9:16 AM To: Fellow HistoNetters Subject: [Histonet] CAP They're here!!!!!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From Stacy_McLaughlin <@t> cooley-dickinson.org Wed May 30 09:58:35 2012 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Wed May 30 09:59:11 2012 Subject: [Histonet] CAP In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> Message-ID: I looked at the message before I looked at the sender and thought they were in my facility! (our window is open too) Pulse returning to normal. Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 Stacy_McLaughlin@Cooley-Dickinson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, May 30, 2012 10:16 AM To: Fellow HistoNetters Subject: [Histonet] CAP They're here!!!!!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed May 30 10:03:27 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed May 30 10:04:37 2012 Subject: [Histonet] Update on Slide Printers Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF599@mmc-mail.ad.mhsil.com> We were experiencing a lot of problems with our slide labels and assumed the problems were with the General Data Cognitive Printers. I had asked several of you if you had experienced any problems with these printers while using CoPath Plus automated barcoded tracking. Today after trouble shooting we have found the problems are not with the printers but with the software communicating to the printers from CoPath Plus ABT. I didn't get a lot of response but wanted others to know that the printers were not the problem. If you are experiencing any problems with uneven printing and are using the same pathology system please let me know. Hopefully further upgrades in the systems will resolve any further problems. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From carrie.schray <@t> gmail.com Wed May 30 10:29:23 2012 From: carrie.schray <@t> gmail.com (Carrie Schray) Date: Wed May 30 10:29:28 2012 Subject: [Histonet] QIHC In-Reply-To: <000f01cd3e71$800bdca0$802395e0$@grics.net> References: <000f01cd3e71$800bdca0$802395e0$@grics.net> Message-ID: The Michigan Soceity of Histotechnologists has just completed a QIHC study guide. Please go to our website for more information. www.mihisto.org Thanks! Carrie Schray On 5/30/12, Rae Staskiewicz wrote: > Is there any other resource for information and studying for the QIHC exam > other than the ASCP outline? > > > > Rae Staskiewicz > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jessica <@t> nsh.org Wed May 30 10:36:45 2012 From: Jessica <@t> nsh.org (Jessica Smith) Date: Wed May 30 10:36:53 2012 Subject: [Histonet] NSH IHC/ISH Two-Day Forum Message-ID: <2B6E973D7D12964F8D8A3598557C553008CDA2@NSH-SRVR01.nsh.local> Good Afternoon! The National Society for Histotechnology is offering a Two-Day Forum focusing on both basic and advance topics in Immunohistochemistry (IHC) and In Situ Hybridization (ISH). The event will be held Friday, July 13 & Saturday, July 14 at the Hartford/Windsor Marriott in Windsort, CT! So come join us for an outstanding two day program with some of the best speakers for one low flat rate! For more details and to register visit: http://s3.goeshow.com/nsh/IHC2012/ereg852712.cfm?clear Topics Include: IHC & ISH Overviews, Biospecimen Collection for Immunohistochemical & Molecular Testing, Standardization & Quality Control in Immunohistochemistry, Immunoflouresence, Digital Pathology, IHC Troubleshooting, Selection of Automated IHC & ISH Slide Staining Systems, Estrogen Receptor IHC Analysis and the Implementation of Standardization Guidelines, & Current Issues in Diagnostic Immunohistochemistry Feel free to contact the NSH Office, 443.535.4060 or histo@nsh.org with any additional questions or concerns about the IHC/ISH Forum! We hope to see you in Connecticut! Jessica Smith Meeting Coordinator/Social Media Specialist National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From Jennifer.Bull <@t> northwestpathology.com Wed May 30 12:07:08 2012 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Wed May 30 12:07:17 2012 Subject: [Histonet] PathCentral LIS Message-ID: <85760CECEC18444BB95F26D5E88DAEAA2396472321@hinet2.hinet.org> Are there any labs out there that are currently using PathCentral as an LIS? Any feedback is appreciated. Thanks! mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- From Kimberly.Blundon <@t> drdc-rddc.gc.ca Wed May 30 12:11:30 2012 From: Kimberly.Blundon <@t> drdc-rddc.gc.ca (Blundon, Kimberly) Date: Wed May 30 12:11:40 2012 Subject: [Histonet] Unsubscribe Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501EFE451@suffieldex01.suffield.drdc-rddc.gc.ca> Unsubscribe please. Thank you. Kimberly Blundon B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.blundon@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From mpence <@t> grhs.net Wed May 30 12:15:39 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 30 12:15:42 2012 Subject: [Histonet] CoPath Users using Dragon Software Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E34@is-e2k3.grhs.net> I am looking for anyone that is using CoPath Sunquest and using Dragon software at gross and by the pathologist. Also what are you using for hands-free dictation at gross and at sign out? You can contact me offline if you wish. Thanks, Mike From joelleweaver <@t> hotmail.com Wed May 30 12:20:49 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 30 12:21:05 2012 Subject: [Histonet] QIHC In-Reply-To: <000f01cd3e71$800bdca0$802395e0$@grics.net> References: <000f01cd3e71$800bdca0$802395e0$@grics.net> Message-ID: I used the Dako guides, there is a good theory one and also an AR guide. That is about all I used to study. They are on their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: raestask@grics.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 30 May 2012 09:35:43 -0500 > Subject: [Histonet] QIHC > > Is there any other resource for information and studying for the QIHC exam > other than the ASCP outline? > > > > Rae Staskiewicz > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yjoneschavez <@t> gmail.com Wed May 30 12:34:25 2012 From: yjoneschavez <@t> gmail.com (Yvonne) Date: Wed May 30 12:34:35 2012 Subject: [Histonet] Please unsubscribe Message-ID: <76C45466-611D-481D-85C1-26CED4FA7D3B@gmail.com> Please unsubscribe me from this list. Sent from my iPod From Kimberly.Blundon <@t> drdc-rddc.gc.ca Wed May 30 12:51:13 2012 From: Kimberly.Blundon <@t> drdc-rddc.gc.ca (Blundon, Kimberly) Date: Wed May 30 12:51:18 2012 Subject: [Histonet] Unsubscribe Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501EFE452@suffieldex01.suffield.drdc-rddc.gc.ca> Please unsubscribe me from this list. Thank you Kimberly Blundon B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.blundon@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada From joelleweaver <@t> hotmail.com Wed May 30 12:52:55 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 30 12:53:06 2012 Subject: [Histonet] compatibility of Thermo slide printers with Leica cassette printer In-Reply-To: References: Message-ID: I have used the code printed cassettes, but with QR codes. I have seen the slide printing only but not in use (yet). In theory any reader should be able to read the standard symbologies. But since they are different vendors, you might contact them directly about compatability for transfer of patient ID data. Maybe middleware is an option if they are not directly compatible. ,Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 29 May 2012 15:12:07 -0500 > From: Sandra.Harrison3@va.gov > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] compatibility of Thermo slide printers with Leica cassette printer > > 1) Does anyone have a Leica Cassette Printer on which they are > printing 2-Dimensional barcodes? > > > > 2) Is anyone using a Thermo slide printer, with bar code reader, to > read cassettes printed off of a Leica Cassette Printer with 2-D bar > codes? > > > > Thanks for your input. > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Wed May 30 13:23:22 2012 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Wed May 30 13:23:31 2012 Subject: [Histonet] CoPath Users using Dragon Software In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974E34@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974E34@is-e2k3.grhs.net> Message-ID: <2925AE271EAAD440AF48FCCEB8002D091C0778A9@smgmail01.smgj.sclhs.net> I would be interested in this info, as well - please share! Thank you Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, May 30, 2012 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CoPath Users using Dragon Software I am looking for anyone that is using CoPath Sunquest and using Dragon software at gross and by the pathologist. Also what are you using for hands-free dictation at gross and at sign out? You can contact me offline if you wish. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Jessica.Vacca <@t> HCAhealthcare.com Wed May 30 13:35:15 2012 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Wed May 30 13:44:37 2012 Subject: [Histonet] EPIC Beaker AP or clinical system Message-ID: <938D716CD445614ABBB817517557B6F4076FA3FD5F@NADCWPMSGCMS09.hca.corpad.net> Is anyone out there on the Epic-Beaker system? I would like to talk to someone that is already live on the system, if you have a few minutes can you contact me at the email below? Thanks Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 813-789-3588 e: Jessica.Vacca@hcahealthcare.com From nmhisto <@t> comcast.net Wed May 30 17:00:30 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Wed May 30 17:00:48 2012 Subject: [Histonet] How to Unsubscribe, Part 114 Message-ID: <1688184558.484887.1338415230136.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Okay, I'm retired now but ?I monitor Histonet from my dining room table while eating bon-bons between soap operas.? Yeah, right.? But that doesn't mean I can't add to the conversation... I would like to remind all subscribers (for the 114th time) that when you subscribed to Histonet, you were given ? UNSUBSCRIBE directions . The listserv manager cannot unsubscribe you - you must do it yourself.? Does it sound like I've mellowed any in the last 60 days?? Not!? I miss my microtome and hope that Carole is talking nicely to it as I did... From prisana.p <@t> psu.ac.th Wed May 30 22:05:31 2012 From: prisana.p <@t> psu.ac.th (prisana.p@psu.ac.th) Date: Wed May 30 22:05:42 2012 Subject: [Histonet] Masson trichrome goldner Message-ID: <9050cb3bd212273eb67f0951c4222f0e.squirrel@webmail.psu.ac.th> Dear I am working on undecalcified section of bone and stain it with masson trichrome goldner. I am not sure aabout the protocol I used or anything wrong because all my bone is red not green. Does anyone has the same problem? Please suggest me what happen and what to do. if you could share the protocol it will be very appreciated. yours sincerely Prisana From info <@t> morphisto.de Thu May 31 00:15:54 2012 From: info <@t> morphisto.de (Morphisto GmbH) Date: Thu May 31 00:16:07 2012 Subject: [Histonet] Masson trichrome goldner In-Reply-To: <9050cb3bd212273eb67f0951c4222f0e.squirrel@webmail.psu.ac.th> References: <9050cb3bd212273eb67f0951c4222f0e.squirrel@webmail.psu.ac.th> Message-ID: <458F586C-B407-4803-999E-91A1A9DC3C57@morphisto.de> Dear Prisana, we often stain bones and other similiar tissues with masson goldner, but we never had the problem, that the bone becomes red instead of green or blue (depending if you use light green or aniline blue). We use the protocol which you can find here in this list: http://www.morphisto.de/en/histology/methods/ The protocol and the names of the solutions are in german, but I think it will be no problem, to understand it. Best regards Michael Am 31.05.2012 um 05:05 schrieb prisana.p@psu.ac.th: > Dear > I am working on undecalcified section of bone and stain it with masson > trichrome goldner. I am not sure aabout the protocol I used or anything > wrong because all my bone is red not green. Does anyone has the same > problem? Please suggest me what happen and what to do. if you could share > the protocol it will be very appreciated. > yours sincerely > Prisana > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 60 Telefax: 069 / 989 72 1100 E-Mail: info@morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. 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From MSHERWOOD <@t> PARTNERS.ORG Thu May 31 08:55:21 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu May 31 08:59:53 2012 Subject: [Histonet] How to Unsubscribe, Part 114 In-Reply-To: <1688184558.484887.1338415230136.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> References: <1688184558.484887.1338415230136.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <090FA56107A969459F3941DDD5585C3A1175F6D8@PHSX10MB10.partners.org> I love it! How true. I get frustrated as well. Enjoy your retirement! Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Wednesday, May 30, 2012 6:01 PM To: HISTONET Subject: [Histonet] How to Unsubscribe, Part 114 Okay, I'm retired now but ?I monitor Histonet from my dining room table while eating bon-bons between soap operas.? Yeah, right.? But that doesn't mean I can't add to the conversation... I would like to remind all subscribers (for the 114th time) that when you subscribed to Histonet, you were given ? UNSUBSCRIBE directions . The listserv manager cannot unsubscribe you - you must do it yourself.? Does it sound like I've mellowed any in the last 60 days?? Not!? I miss my microtome and hope that Carole is talking nicely to it as I did... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From b-shmaltsuyev <@t> northwestern.edu Thu May 31 10:13:58 2012 From: b-shmaltsuyev <@t> northwestern.edu (Bella Shmaltsuyeva) Date: Thu May 31 10:14:07 2012 Subject: [Histonet] Please unsubscribe Message-ID: Please unsubscribe me from this list. Bella Shmaltsuyeva, HT (ASCP),QIHC Senior Research Tech Pathology Core Facility Robert H Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-429 Chicago ,IL6061 312-503-4705 b-shmaltsuyev@northwestern.edu From brett_connolly <@t> merck.com Thu May 31 10:18:23 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu May 31 10:18:36 2012 Subject: [Histonet] RE: Please unsubscribe In-Reply-To: References: Message-ID: # 115 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bella Shmaltsuyeva Sent: Thursday, May 31, 2012 11:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Please unsubscribe Please unsubscribe me from this list. Bella Shmaltsuyeva, HT (ASCP),QIHC Senior Research Tech Pathology Core Facility Robert H Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-429 Chicago ,IL6061 312-503-4705 b-shmaltsuyev@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From dtaylor <@t> mcpathology.com Thu May 31 10:34:52 2012 From: dtaylor <@t> mcpathology.com (Debbie Taylor) Date: Thu May 31 10:38:57 2012 Subject: [Histonet] please unsubscribe Message-ID: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> Please unsubscribe me from this list. Thanks. Deborah Taylor, MS Customer Relations/Lab Manager Marlboro Chesterfield Pathology, PC 672 Hwy 9 West Bennettsville, SC 29512 Phone: 843-479-2402 Fax: 843-479-6609 Note: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anone else is unauthorized. If you are not the intended recipient, any disclosure copying or distribution of the message or any action or omission taken by you in reliance on it, is prohibitied and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. From wdesalvo.cac <@t> hotmail.com Thu May 31 10:40:06 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu May 31 10:40:15 2012 Subject: [Histonet] please unsubscribe In-Reply-To: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> References: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> Message-ID: #116 - WHEN WILL IT STOP????? William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 31 May 2012 11:34:52 -0400 > From: dtaylor@mcpathology.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] please unsubscribe > > Please unsubscribe me from this list. Thanks. > > > > Deborah Taylor, MS > > Customer Relations/Lab Manager > > Marlboro Chesterfield Pathology, PC > > 672 Hwy 9 West > > Bennettsville, SC 29512 > > Phone: 843-479-2402 > > Fax: 843-479-6609 > > > > > > Note: The information in this message is confidential and may be > legally privileged. It is intended solely for the addressee. Access to > this message by anone else is unauthorized. If you are not the intended > recipient, any disclosure copying or distribution of the message or any > action or omission taken by you in reliance on it, is prohibitied and > may be unlawful. Please immediately contact the sender if you have > received this message in error. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask <@t> grics.net Thu May 31 10:40:54 2012 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Thu May 31 10:40:58 2012 Subject: [Histonet] ADH 5 Message-ID: <20120531114054.ru0s93ll70n408g4@webmail2.centurytel.net> Is anyone doing the ADH 5 stain on a Ventana platform? Rae Staskiewicz Methodist Medical Center in Illinois From jqb7 <@t> cdc.gov Thu May 31 10:41:10 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu May 31 10:41:26 2012 Subject: [Histonet] please unsubscribe In-Reply-To: References: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> Message-ID: Too funny! Everyone, UNSUBSCRIBE YOURSELF! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, May 31, 2012 11:40 AM To: dtaylor@mcpathology.com; histonet Subject: RE: [Histonet] please unsubscribe #116 - WHEN WILL IT STOP????? William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 31 May 2012 11:34:52 -0400 > From: dtaylor@mcpathology.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] please unsubscribe > > Please unsubscribe me from this list. Thanks. > > > > Deborah Taylor, MS > > Customer Relations/Lab Manager > > Marlboro Chesterfield Pathology, PC > > 672 Hwy 9 West > > Bennettsville, SC 29512 > > Phone: 843-479-2402 > > Fax: 843-479-6609 > > > > > > Note: The information in this message is confidential and may be > legally privileged. It is intended solely for the addressee. Access to > this message by anone else is unauthorized. If you are not the > intended recipient, any disclosure copying or distribution of the > message or any action or omission taken by you in reliance on it, is > prohibitied and may be unlawful. Please immediately contact the sender > if you have received this message in error. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu May 31 12:03:28 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu May 31 12:03:32 2012 Subject: [Histonet] please unsubscribe #117 In-Reply-To: References: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> Message-ID: Bill, It will never stop as long as people don't pay attention to the instructions when they subscribe. Maybe the process is too difficult for some people? Maybe they don't understand the instructions? Sally, Have you considered sending out weekly synopses of the soaps so we can all keep up while we still have to come to work? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From Vickroy.Jim <@t> mhsil.com Thu May 31 13:48:26 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu May 31 13:49:33 2012 Subject: [Histonet] BLock alignment tool Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com> Awhile back we got an advertisement for a block alignment tool that would be helpful in recut cases. Can anyone share with me companies that have this device and approximate costs? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From campbellj <@t> muhlbauerlab.com Thu May 31 13:56:48 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Thu May 31 13:57:25 2012 Subject: [Histonet] BLock alignment tool In-Reply-To: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com> Message-ID: American MasterTech carries it and I believe it was ~$200. Newcomer Supply had it too but I think the price was higher from them. On Thu, May 31, 2012 at 2:48 PM, Vickroy, Jim wrote: > Awhile back we got an advertisement for a block alignment tool that would > be helpful in recut cases. Can anyone share with me companies that have > this device and approximate costs? > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From foreightl <@t> gmail.com Thu May 31 13:56:59 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Thu May 31 13:57:28 2012 Subject: [Histonet] BLock alignment tool In-Reply-To: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com> Message-ID: Newcomer supply has one that works in all standard microtomes. It is rather intuitive. It is essentially a level on an apparatus that goes inside of the block clamp part of the microtome. They call it the universal microtome alignment tool. On Thu, May 31, 2012 at 11:48 AM, Vickroy, Jim wrote: > Awhile back we got an advertisement for a block alignment tool that would > be helpful in recut cases. Can anyone share with me companies that have > this device and approximate costs? > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From b-frederick <@t> northwestern.edu Thu May 31 14:10:11 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu May 31 14:10:17 2012 Subject: [Histonet] BLock alignment tool In-Reply-To: References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E254CB8@evcspmbx3.ads.northwestern.edu> As does TechOne Biomedical. www.techoneweb.com. We have one for each microtome. Just bear in mind if your floor or workstation is not level, it may skew the leveling of the microtome. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Thursday, May 31, 2012 1:57 PM To: Vickroy, Jim Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] BLock alignment tool Newcomer supply has one that works in all standard microtomes. It is rather intuitive. It is essentially a level on an apparatus that goes inside of the block clamp part of the microtome. They call it the universal microtome alignment tool. On Thu, May 31, 2012 at 11:48 AM, Vickroy, Jim wrote: > Awhile back we got an advertisement for a block alignment tool that would > be helpful in recut cases. Can anyone share with me companies that have > this device and approximate costs? > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical > Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should > delete this message. Any disclosure, copying, or distribution of this > message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Thu May 31 14:16:41 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu May 31 14:16:46 2012 Subject: [Histonet] BLock alignment tool In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E254CB8@evcspmbx3.ads.northwestern.edu> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com>, , <62C639732D3F274DACED033EBDF6ADAF1E254CB8@evcspmbx3.ads.northwestern.edu> Message-ID: Good point. That is why I like the tools that slip onto the stage. A little more money up front, but much quicker to align multiple microtomes and all microtome chucks are exactly the same. Which, I think, should be the goal. William DeSalvo, B.S., HTL(ASCP) > From: b-frederick@northwestern.edu > To: foreightl@gmail.com; Vickroy.Jim@mhsil.com > Date: Thu, 31 May 2012 19:10:11 +0000 > Subject: RE: [Histonet] BLock alignment tool > CC: histonet@lists.utsouthwestern.edu > > As does TechOne Biomedical. www.techoneweb.com. We have one for each microtome. Just bear in mind if your floor or workstation is not level, it may skew the leveling of the microtome. > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie > Sent: Thursday, May 31, 2012 1:57 PM > To: Vickroy, Jim > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] BLock alignment tool > > Newcomer supply has one that works in all standard microtomes. It is rather intuitive. It is essentially a level on an apparatus that goes inside of the block clamp part of the microtome. They call it the universal microtome alignment tool. > > On Thu, May 31, 2012 at 11:48 AM, Vickroy, Jim wrote: > > > Awhile back we got an advertisement for a block alignment tool that would > > be helpful in recut cases. Can anyone share with me companies that have > > this device and approximate costs? > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical > > Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > > information intended for a specific individual and purpose, and is > > protected by law. If you are not the intended recipient, you should > > delete this message. Any disclosure, copying, or distribution of this > > message, or the taking of any action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrie.schray <@t> gmail.com Thu May 31 18:16:49 2012 From: carrie.schray <@t> gmail.com (Carrie Schray) Date: Thu May 31 18:16:55 2012 Subject: [Histonet] QIHC In-Reply-To: <000f01cd3e71$800bdca0$802395e0$@grics.net> References: <000f01cd3e71$800bdca0$802395e0$@grics.net> Message-ID: Also available from the Michigan Society of Histotechnologists are a HT/HTL study guide and a Competency Assessment Handbook. They really are great to have to help your staff pass their exam, as well as in developing competency assessments to meet your inspection criteria! The competency assessment has example forms and content of forms that are useful when integrating assessments in your laboratory. For your conveinence, our website is www.mihisto.org. Thank you, Carrie Schray Secretary-elect, Michigan Soceity of Histotechnologists On 5/30/12, Rae Staskiewicz wrote: > Is there any other resource for information and studying for the QIHC exam > other than the ASCP outline? > > > > Rae Staskiewicz > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >