From mpence <@t> grhs.net Thu Mar 1 08:20:55 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Mar 1 08:21:03 2012 Subject: [Histonet] Sunquest CoPath Listserver? In-Reply-To: <625573D1-97A8-4419-A185-2D77D7F0B9AC@mac.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974DC6@is-e2k3.grhs.net> I do know that there is a listserv for CoPath, but it has been a few years ago that I belonged to it. They monitor everything that is said on it and do not allow anything that is negative toward CoPath or any problems with their product. I did not see much use if I could not get the truth about a problem or their product! Good luck! Mike Pence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Watkins David Sent: Wednesday, February 29, 2012 4:55 PM To: histonet@lists.utsouthwestern.edu Cc: Watkins David Subject: [Histonet] Sunquest CoPath Listserver? Does anyone know if there is a Sunquest CoPath listserv? We are upgrading to v.6, and I would like to mine it for information about this new version. thx, David Watkins, MD Department of Pathology Baylor University Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Mar 1 08:28:18 2012 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Mar 1 08:28:28 2012 Subject: [Histonet] Congo red Message-ID: Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From brett_connolly <@t> merck.com Thu Mar 1 08:45:49 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Mar 1 08:45:59 2012 Subject: [Histonet] RE: Congo red In-Reply-To: References: Message-ID: I think it works better with thicker sections ( 8-10um) Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, March 01, 2012 9:28 AM To: histonet Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Congo red Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From TGoins <@t> mt.gov Thu Mar 1 09:25:35 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Mar 1 09:25:46 2012 Subject: [Histonet] RE: Congo red In-Reply-To: References: Message-ID: Cut at 8-10 microns - I believe birefringence depends on the increased thickness of the tissue. We stain our sections with Congo Red for 5 min with good color and fluorescent results. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, March 01, 2012 7:28 AM To: histonet Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Congo red Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Thu Mar 1 09:55:38 2012 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Mar 1 09:55:48 2012 Subject: [Histonet] new antibody validation Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD4CF@EXCHANGESB> ANP.22750 The laboratory has documented validation of new antibodies, prior to use in patient diagnosis. In the CAP's note it states that a panel of 10 positive and 10 negative neoplasms would be sufficient for a well-characterized antibody. How extensive a panel of tissues are laboratories using to meet this checklist requirement? Is it necessary to do 10 positive and 10 negatives or can it be at the discretion of the laboratory director? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From JWeems <@t> sjha.org Thu Mar 1 10:43:50 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 1 10:44:04 2012 Subject: [Histonet] RE: Congo red In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640E0E0FB846@CHEXCMS10.one.ads.che.org> I would cut at 8-10 microns. See how that works. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Please note: New email address effective 3/2/12: Joyce.Weems@emoryhealthcare.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, March 01, 2012 09:28 To: histonet Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Congo red Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From LSebree <@t> uwhealth.org Thu Mar 1 10:46:09 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Mar 1 10:46:13 2012 Subject: [Histonet] new antibody validation In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD4CF@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD4CF@EXCHANGESB> Message-ID: We have been picking (or trying to) 10 positive cases that have negative tissue elements included. Obviously for some hard-to-find positive cases we need to make due with what's available. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, March 01, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new antibody validation ANP.22750 The laboratory has documented validation of new antibodies, prior to use in patient diagnosis. In the CAP's note it states that a panel of 10 positive and 10 negative neoplasms would be sufficient for a well-characterized antibody. How extensive a panel of tissues are laboratories using to meet this checklist requirement? Is it necessary to do 10 positive and 10 negatives or can it be at the discretion of the laboratory director? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu Mar 1 11:22:56 2012 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Mar 1 11:23:05 2012 Subject: [Histonet] Congo red In-Reply-To: References: Message-ID: <4F4FB070.1030704@shaw.ca> Bennhold's method is not the easiest to give good results. Try Highmans's method (http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm). It is much easier to control. Remember that congo red is not an intensely coloured dye and the results are often pale. If your pathologists want a punchier stain try sirius red F3B (NOT 4B), as this is a deeper red than congo red. It can be used in a Highman type procedure as a direct substitute for congo red. It also gives green birefringence, again somewhat darker than congo red. Bryan Llewellyn Cheri Miller wrote: > Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. > > Cheryl A. Miller HT(ASCP)cm > Histology/Cytology Prep Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4145 ext. 554 > > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren <@t> gmail.com Thu Mar 1 13:04:43 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Mar 1 13:04:49 2012 Subject: [Histonet] Congo red In-Reply-To: <4F4FB070.1030704@shaw.ca> References: <4F4FB070.1030704@shaw.ca> Message-ID: If it's taking you a week, you're doing it wrong. Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) ______________________________**_________________ Histonet mailing list Histonet@lists.utsouthwestern.**edu http://lists.utsouthwestern.**edu/mailman/listinfo/histonet ______________________________**_________________ Histonet mailing list Histonet@lists.utsouthwestern.**edu http://lists.utsouthwestern.**edu/mailman/listinfo/histonet From Elizabeth.Cameron <@t> jax.org Thu Mar 1 13:27:49 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Thu Mar 1 13:27:55 2012 Subject: [Histonet] RE: Jones/PAMS Message-ID: Just wanted to give a quick update on this. I had a suggestion for using thiosemicarbazide for 10 minutes after the periodic acid, and of all the things we tried, this was the only thing that worked. It eliminated the nuclear staining and the capillaries are now picking up the silver (as they should be!). Unfortunately, I accidently deleted the email that suggested this so I don't know who to thank, but it was a great suggestion! Liz From: Elizabeth Cameron Sent: Thursday, February 16, 2012 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: Jones/PAMS Hi, I was wondering if anyone has any suggestions for a Jones/PAMS stain that is not working properly. This is something we don't do often. The last time we did it was a year and a half ago, and it seemed fine at the time. We have tried 3 or 4 protocols, including an ammoniacal silver, and it is still not working properly. In some protocols, our red cells are staining but the capillaries in the glomeruli do not seem to be picking up the silver. In other protocols, there are nuclei of some cells that should not be staining that are, but again, the capillaries are not. We are working on mouse tissue that is fixed in NBF. The strange thing is the stain seems to be working well on Bouins and Telly's fixed tissue. I even tried mordanting in Bouins! We have tried multiple kidneys with the same results. We are on new bottles of silver and periodic acid, although our methenamine has been around a while. Any suggestions would be greatly appreciated. Thanks! Liz From rjbuesa <@t> yahoo.com Thu Mar 1 14:01:32 2012 From: rjbuesa <@t> yahoo.com (rjbuesa@yahoo.com) Date: Thu Mar 1 14:01:38 2012 Subject: [Histonet] blackbomber2006 Message-ID: <1330632092.31086.yint-ygo-j2me@web162104.mail.bf1.yahoo.com> Make Money 0nIine Fast http://72pkr.com/madnecy.php?ifahforum=46 Thu, 1 Mar 2012 21:01:31 _________________________________ " The place to which Isaac had retreated was a city which he possessed in the interior of the island called Nicosia" (c) aimil cf amelia aelfwine From one_angel_secret <@t> yahoo.com Thu Mar 1 14:13:39 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Mar 1 14:13:51 2012 Subject: [Histonet] Sunquest CoPath Listserver? In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974DC6@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974DC6@is-e2k3.grhs.net> Message-ID: <31F53ACD-CF37-4314-B0D3-C275043F3008@yahoo.com> Last time and it's been a while that I went on the Cerner copath list serve there wasn't much action. We were sunquest copath to at the time. If you don't see what you need on cerners site perhaps you could ask them to give you a reference of someone who has a sunquest copath system for you to call. I've found that helpful at times. Hood luck. Kim D Sent from my iPhone On Mar 1, 2012, at 9:20 AM, "Mike Pence" wrote: > I do know that there is a listserv for CoPath, but it has been a few > years ago that I belonged to it. They monitor everything that is said on > it and do not allow anything that is negative toward CoPath or any > problems with their product. I did not see much use if I could not get > the truth about a problem or their product! Good luck! > Mike Pence > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Watkins > David > Sent: Wednesday, February 29, 2012 4:55 PM > To: histonet@lists.utsouthwestern.edu > Cc: Watkins David > Subject: [Histonet] Sunquest CoPath Listserver? > > > Does anyone know if there is a Sunquest CoPath listserv? > We are upgrading to v.6, and I would like to mine it for information > about this new version. > > thx, > > David Watkins, MD > Department of Pathology > Baylor University Medical Center > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Mar 1 14:24:54 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Mar 1 14:24:57 2012 Subject: [Histonet] Re: Congo red Message-ID: Some more points about trouble-shooting a weak Congo red stain: Sections are commonly cut at 8 to 10 ?m, as several people have noted. I think that amyloid deteriorates on slides stored when they're cut but not deparaffinized - in something like a month. Your control may simply stain weakly, however you stain it. Good amyloid controls are hard to get. What tissue does your control slide come from? Lately most of the ones I've seen are taken from human amyloid-containing medullary thyroid carcinomas. Are your pathologists using polarization to look at the slides? Amyloid stains require a high quality polarizer, which pathologists often aren't allowed to have. Finally, I've asked these two questions on Histonet several times, and never had a response: 1. Amyloid is fairly easily produced in experimental animals such as mice. Is this material ever used as an amyloid control? 2. Has anybody tried Anatech's "Amyloid Red" stain? Bob Richmond Samurai Pathologist Knoxville TN From Diane.Tokugawa <@t> kp.org Thu Mar 1 16:50:07 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Thu Mar 1 16:50:20 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 03/01/2012 and will not return until 03/08/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From tuyenmai77 <@t> yahoo.com Thu Mar 1 17:48:42 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Thu Mar 1 17:48:46 2012 Subject: [Histonet] blocking endogenous activities Message-ID: <1330645722.91952.YahooMailClassic@web162801.mail.bf1.yahoo.com> In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied before antigen heat retrieval. However, I wonder there is any different result in IHC stain if hydrogen peroxide for blocking endogenous activities is applied after antigen heat retrieval by using steamer or pressure cooker? Thank you, Mai Nguyen Truong Research Associate II Los Angeles, California From BDeBrosse-Serra <@t> isisph.com Thu Mar 1 17:55:06 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Mar 1 17:55:17 2012 Subject: [Histonet] blocking endogenous activities In-Reply-To: <1330645722.91952.YahooMailClassic@web162801.mail.bf1.yahoo.com> References: <1330645722.91952.YahooMailClassic@web162801.mail.bf1.yahoo.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F0921436376@EXCHMB01.isis.local> The majority of the time we do it after retrieval. I believe you can do the hydrogen peroxide block at any point, as long as you do it before the DAB. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen Sent: Thursday, March 01, 2012 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blocking endogenous activities In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied before antigen heat retrieval. However, I wonder there is any different result in IHC stain if hydrogen peroxide for blocking endogenous activities is applied after antigen heat retrieval by using steamer or pressure cooker? Thank you, Mai Nguyen Truong Research Associate II Los Angeles, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu Mar 1 18:13:28 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Mar 1 18:13:32 2012 Subject: [Histonet] blocking endogenous activities In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F0921436376@EXCHMB01.isis.local> References: <1330645722.91952.YahooMailClassic@web162801.mail.bf1.yahoo.com> <493CAA64F203E14E8823737B9EE0E25F0921436376@EXCHMB01.isis.local> Message-ID: You need to do it sometime before the HRP-labeled conjugate is put on. If you put it on after the HRP conjugate, you'll block the HRP substrate and won't see where the primary antibody is binding. I usually do mine before antigen retrieval, but have done it after the primary antibody step on frozen sections and cell smears. Jan Shivers Senior Scientist IHC/Histology/EM Veterinary Diagnostic Lab University of Minnesota St. Paul, MN On Thu, Mar 1, 2012 at 5:55 PM, Bea DeBrosse-Serra < BDeBrosse-Serra@isisph.com> wrote: > The majority of the time we do it after retrieval. I believe you can do > the hydrogen peroxide block at any point, as long as you do it before the > DAB. > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2588 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen > Sent: Thursday, March 01, 2012 3:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] blocking endogenous activities > > In my lab, in IHC protocols for most antibodies, hydrogen peroxide is > applied before antigen heat retrieval. However, I wonder there is any > different result in IHC stain if hydrogen peroxide for blocking endogenous > activities is applied after antigen heat retrieval by using steamer or > pressure cooker? > Thank you, > Mai Nguyen Truong > Research Associate II > Los Angeles, California > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From foreightl <@t> gmail.com Thu Mar 1 19:14:57 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Thu Mar 1 19:15:04 2012 Subject: [Histonet] new antibody validation In-Reply-To: References: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD4CF@EXCHANGESB> Message-ID: Based off the statement: *The scope of the validation is at the discretion of the laboratory director and will vary with the antibody* It seems that it gives the laboratory director the ability to give specific qualifications for each antibody. If a laboratory director says 2 positive cases and 1 negative case is appropriate for validation (I can't believe that there would be one), CAP wouldn't cite them if they had a specific written and signed procedure for this. We try to follow the recommendations by Goldstein et. al. 2007, "Recommendations for improved standardization of immunohistochemistry" from applied immunohistochemistry and molecular morphology. Paraphrasing from recommendation #9: 10 samples high levels of target antigen, 10 samples of intermediate to low target antigen and 5 samples of no IHC evidence of target antigen. On Thu, Mar 1, 2012 at 8:46 AM, Sebree Linda A wrote: > We have been picking (or trying to) 10 positive cases that have negative > tissue elements included. Obviously for some hard-to-find positive > cases we need to make due with what's available. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol > Bryant > Sent: Thursday, March 01, 2012 9:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] new antibody validation > > ANP.22750 > The laboratory has documented validation of new antibodies, prior to use > in patient diagnosis. > In the CAP's note it states that a panel of 10 positive and 10 negative > neoplasms would be sufficient for a well-characterized antibody. > > How extensive a panel of tissues are laboratories using to meet this > checklist requirement? Is it necessary to do 10 positive and 10 > negatives or can it be at the discretion of the laboratory director? > > Carol Bryant, CT (ASCP) > Cytology/Histology Manager > Lexington Clinic > Phone (859) 258-4082 > Fax (859) 258-4081 > cbrya@lexclin.com > > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole > use of the addressee and may contain confidential or privileged > information that is protected by the State of Kentucky and/or Federal > regulations. If you are not the intended recipient, do not read, copy, > retain or disseminate this message or any attachment. If you have > received this message in error, please call the sender immediately at > (859)258-4000 and delete all copies of this message and any attachment. > Any unauthorized review, use, disclosure, copying or distribution is > strictly prohibited. Neither the transmission of this message or any > attachment, nor any error in transmission or misdelivery shall > constitute waiver of any applicable legal privilege. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From James.Reilly <@t> glasgow.ac.uk Fri Mar 2 02:54:09 2012 From: James.Reilly <@t> glasgow.ac.uk (Jim Reilly) Date: Fri Mar 2 02:54:24 2012 Subject: [Histonet] Shiny side of a paraffin section Message-ID: Hello Everyone I have worked in histology for 41 years and the only reason we ever put sections shiny side up is when we want to look at back to back sections: We would take the first section and put it shiny side up in the bath and the next section immediately after would be placed shiny side down. This will give you adjacent sections that will be cut through the same face i.e. if a cell is cut in half the other half will be mirrored on the other section. This can be a very handy tool for comparing IHC staining. Cheers James H Reilly Senior Histology Technician Institute Of Infection, Immunity, Inflammation College Of Medical, Veterinary and Life Sciences University Of Glasgow Room B4/27 Sir Graeme Davies Building 120 University Place Glasgow G12 8TA Tel: +44 141 330 8420/7573 The University of Glasgow is a charity registered in Scotland, charity number SC004401 From lpwenk <@t> sbcglobal.net Fri Mar 2 04:35:07 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Mar 2 04:35:11 2012 Subject: [Histonet] Congo red In-Reply-To: <4F4FB070.1030704@shaw.ca> References: <4F4FB070.1030704@shaw.ca> Message-ID: <0D609432FF7740A496F8EC9167BD5482@HP2010> Is it ONE particular case that is giving you problems, or ALL cases of amyloid? Maybe just the control? If the amyloid is a large deposit, that has been in the patient for a long time, the beta pleats can get warped, and the Congo red will be very pale to no staining. In those cases, we: - Use the Auramine-Rhodamine fluorescence scope (hit slide with green light) on the Congo red stained tissue. Congo red amyloid will fluoresce orange against a black background. - Do a crystal violet stain. Amyloid will be pink violet against a blue purple background. - Do a Thioflavin T stain. Use the FITC fluorescence microscope (hit slide with blue light). TFT will fluoresce yellow against a black background. All the amyloid stains have a weakness when it comes to staining amyloid. There isn't one that works all the time. If the Congo red is working fine, then that's the only stain we do. But if the Congo red isn't staining correctly (our control is great, but the patient's tissue is weak to no staining), then we do one or more of the above options. If you need the crystal violet or TFT procedure, let me know. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of my employer. -----Original Message----- From: Bryan Llewellyn Sent: Thursday, March 01, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Congo red Bennhold's method is not the easiest to give good results. Try Highmans's method (http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm). It is much easier to control. Remember that congo red is not an intensely coloured dye and the results are often pale. If your pathologists want a punchier stain try sirius red F3B (NOT 4B), as this is a deeper red than congo red. It can be used in a Highman type procedure as a direct substitute for congo red. It also gives green birefringence, again somewhat darker than congo red. Bryan Llewellyn Cheri Miller wrote: > Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% > aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 > microns. And I leave in the solution for up to 4 hours and the paths are > still saying it is weak. Any ideas? I have even stopped the Alkaline > alcohol differentiation step and its still too weak. > > Cheryl A. Miller HT(ASCP)cm > Histology/Cytology Prep Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4145 ext. 554 > > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 2 09:24:10 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 2 09:24:19 2012 Subject: [Histonet] blocking endogenous activities Message-ID: <1330701850.69627.YahooMailClassic@web162106.mail.bf1.yahoo.com> Peroxidase, the enzyme you want to block with hydrogen peroxide, is a protein and, as such, should be cross-linked by formalin if the tissue is properly sixed. In consequence, you should eliminate the crosslinkage to fully expose the enzyme before blocking it. Therefore, you should do HIER first and block the peroxidase afterwards. On the other hand, many labs do Ren? J. --- On Thu, 3/1/12, Tuyen Nguyen wrote: From: Tuyen Nguyen Subject: [Histonet] blocking endogenous activities To: histonet@lists.utsouthwestern.edu Date: Thursday, March 1, 2012, 6:48 PM In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied before antigen heat retrieval. However, I wonder there is any different result in IHC stain if hydrogen peroxide for blocking endogenous activities is applied after antigen heat retrieval by using steamer or pressure cooker? Thank you, Mai Nguyen Truong Research Associate II Los Angeles, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patjnm <@t> gwumc.edu Fri Mar 2 11:26:28 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Fri Mar 2 11:26:35 2012 Subject: [Histonet] Repair techs for very old Shandon Tissue Processor, (Roosevelt was in office) Message-ID: <4F50BC74.DB55.001F.1@gwumc.edu> I have an old Shandon tissue processor that is so old, they just called in tissue processor, I think Kennedy was in office, and the serial number is -10. THe machine has been donated and passed down and I am having a hard time getting to work. I t worked once, but now it just keeps giving me every kind of error possible. In speaking with Shandon they have helped me to a coup-le of different resets, but all it does is cycle and try and find other modules. THis os one of those procssors that might have been part of a sequence of several processors. It keeps looking for other modules. ANyway, I cannot get it to do anything. DOes anyone know of a tech out there who can work on these Shandon Pathcenteres? Many thanks. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From Dorothy.L.Webb <@t> HealthPartners.Com Fri Mar 2 12:06:43 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Mar 2 12:06:48 2012 Subject: [Histonet] microtomy Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Courtney.Pierce <@t> quintiles.com Fri Mar 2 12:31:11 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Fri Mar 2 12:31:20 2012 Subject: [Histonet] AKT and pAKT Message-ID: Has anyone out there done AKT and pAKT antibodies for IHC. I have been working on them for about a month now and they are still not working right. I hope there is someone out there that can help me??? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From Longf1 <@t> LabCorp.com Fri Mar 2 12:35:26 2012 From: Longf1 <@t> LabCorp.com (Long, Florence) Date: Fri Mar 2 12:38:27 2012 Subject: [Histonet] RE: microtomy In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Message-ID: Make sure all levers and parts of the knife holder are tightened securely, but do not overtighten -especially on the blade holding plate F. Long ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L [Dorothy.L.Webb@HealthPartners.Com] Sent: Friday, March 02, 2012 1:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] microtomy We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From AGleiberman <@t> cbiolabs.com Fri Mar 2 12:41:53 2012 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Fri Mar 2 12:42:00 2012 Subject: [Histonet] blocking endogenous peroxidase Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C7265D592@cbiolabs05.CBiolabs.local> Some peroxidase-like enzymes in macrophages (probably catalase) and DAB-binding activities in erythrocytes (hemoglobin) are not blocked by formalin. The best way to block this activity was incubation with methanol-peroxide (99 parts of methanol-1 part of 30% peroxide) during de-waxing. 3% peroxide is also effective, but in some cases it causes extensive bubbling that can damage fragile sections. So, I am using methanol-peroxide for paraffin sections and stable peroxide buffer (buffer provided in DAB developer kits, such as Pierce metal-enhanced DAB kit) for cryo- sections. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From mehlikafaire <@t> hotmail.com Fri Mar 2 12:58:45 2012 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Fri Mar 2 12:58:50 2012 Subject: [Histonet] AKT and pAKT In-Reply-To: References: Message-ID: I've used cell signaling 4060S XP for pAKT, and it works really well. > From: Courtney.Pierce@quintiles.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 2 Mar 2012 13:31:11 -0500 > Subject: [Histonet] AKT and pAKT > > > Has anyone out there done AKT and pAKT antibodies for IHC. I have been working on them for about a month now and they are still not working right. I hope there is someone out there that can help me??? > > Courtney Pierce > IHC Specialist > Quintiles > Translational R&D - Oncology > Innovation > Navigating the new health > > 610 Oakmont Lane > Westmont, IL 60559 > > Office: + 630-203-6234 > courtney.pierce@quintiles.com > > clinical | commercial | consulting | capital > > > ********************** IMPORTANT--PLEASE READ ************************ > This electronic message, including its attachments, is COMPANY CONFIDENTIAL > and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are > not the intended recipient, you are hereby notified that any use, disclosure, > copying, or distribution of this message or any of the information included > in it is unauthorized and strictly prohibited. If you have received this > message in error, please immediately notify the sender by reply e-mail and > permanently delete this message and its attachments, along with any copies > thereof. Thank you. > ************************************************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 2 14:38:06 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 2 14:38:09 2012 Subject: [Histonet] microtomy In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Message-ID: <1330720686.1552.YahooMailClassic@web162103.mail.bf1.yahoo.com> Cold temperature will shrink the block and determine thinner sections, not thicker sections. The problems has to be on the mechanics or perhaps the block is hotter than you think it is. Ren? J. --- On Fri, 3/2/12, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] microtomy To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, March 2, 2012, 1:06 PM We have run into an interesting scenario and wondering what the "experts" think!? We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome.? Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns.? I had our service technician? measure the microns and the equipment was cutting as set.? I had the blocks cut on a different microtome and we have seen variations there also.? My question is, does the amount of time on ice make a minor difference in the section thickness?? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation!? This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!!? Thank you!! Dorothy Webb, HT (ASCP) ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Fri Mar 2 14:58:50 2012 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Mar 2 14:58:53 2012 Subject: [Histonet] lab. setting up Message-ID: <1330721930.84981.YahooMailNeo@web112616.mail.gq1.yahoo.com> dear all on histonet I'm going to setup a new histology lab.?in Egypt. I need to contact with you to advice me about the best and comfotable facilities microtomes- processors-automatic stainers and so on ..... ? Mohamed Ass. Lec. of histology Faculty of Vet. Med. Cairo University Egypt From rjbuesa <@t> yahoo.com Fri Mar 2 15:28:05 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 2 15:28:09 2012 Subject: [Histonet] lab. setting up In-Reply-To: <1330721930.84981.YahooMailNeo@web112616.mail.gq1.yahoo.com> Message-ID: <1330723685.78638.YahooMailClassic@web162103.mail.bf1.yahoo.com> It would be better if you contact a local sales manager knowledgeable of the local characteristics and ask him/her this question. From here you will probably receive advises about instruments that probably are not available in Egypt. Ren? J. --- On Fri, 3/2/12, mohamed abd el razik wrote: From: mohamed abd el razik Subject: [Histonet] lab. setting up To: "histonet@lists.utsouthwestern.edu" Date: Friday, March 2, 2012, 3:58 PM dear all on histonet I'm going to setup a new histology lab.?in Egypt. I need to contact with you to advice me about the best and comfotable facilities microtomes- processors-automatic stainers and so on ..... ? Mohamed Ass. Lec. of histology Faculty of Vet. Med. Cairo University Egypt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri Mar 2 15:54:23 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Mar 2 15:54:36 2012 Subject: [Histonet] microtomy In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Message-ID: <3EDB2157-842F-4429-BE33-6E6ED784E1F6@yahoo.com> Love your question. Hate to hear that you are having a issue. My two cents follow: Yes. The amount of time for a faced block will effect the section thickness. The cells become bloated if they Sit to long. < feel free to have a Friday laugh on this one. Anyway. If you havnt changed the stain in any way or the tech isn't rushing and hard facing causing extrenuated cell artifact and you don't have bloated cells? Could it be possible his eyesight got better or maybe he is mad and wants to pick a fuss? I sometimes wish we could post pictures if our issues. I'm wishing you the best. Kim D Sent from my iPhone On Mar 2, 2012, at 1:06 PM, "Webb, Dorothy L" wrote: > We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! > > Dorothy Webb, HT (ASCP) > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Mar 2 16:19:02 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Mar 2 16:19:13 2012 Subject: [Histonet] Re: PAMS Jones Basement Membrane Message-ID: <000001ccf8c2$7af210d0$70d63270$@bresnan.net> A stain I have never had a problem with. However I did have the good fortune to listen to Culling many years ago on the use of 1% periodic acid for PAS staining and he insisted periodic acid, no matter what the concentration, should be made fresh every time you do these stains e.g. PAS and PAMS. Some people use 0.5% but we used 1% for 15 minutes, and then did microwave staining for the methenamine silver. A 56C - 60C water bath also works plus monitoring the color of the sections - the color of dark tea. This is NOT a stable oxidizer once made up, and never should be reused under any circumstances. On the chance you have not used this solution in over 1 1/2 years, you may not be getting proper oxidation of the basement membranes since the periodic acid and the methanamine silver may be bad. Also, the Methenamine silver should be no more than 6 months old. We also kept our silver nitrate solution fresh when we made up the methenamine silver solution, and do store our silver nitrate salts in the refrigerator since this is hygroscopic. Check on storage of this salt in MSDS I have never been one to use kits for either of these stains, particularly with periodic acid as a ready to use solution. This goes into solution very rapidly and be sure to oxidize for 10 minutes before going to the methenamine silver. None of these solutions is difficult to make up in house. If you want, I can send my method and also a protocol pdf from HistoLogic by Stanley Shapiro, where he used freshly made 0.5% periodic acid via private email. The nuclei will pick up some silver, but one should be able to discern nuclei from basement membranes on 1 to 2 ?m sections. Good luck Gayle Callis HTL/HT/MT(ASCP) Bozeman MT You wrote: Just wanted to give a quick update on this. I had a suggestion for using thiosemicarbazide for 10 minutes after the periodic acid, and of all the things we tried, this was the only thing that worked. It eliminated the nuclear staining and the capillaries are now picking up the silver (as they should be!). Unfortunately, I accidently deleted the email that suggested this so I don't know who to thank, but it was a great suggestion! Liz From: Elizabeth Cameron Sent: Thursday, February 16, 2012 2:17 PM To: histonet <@t> lists.utsouthwestern.edu Subject: Jones/PAMS Hi, I was wondering if anyone has any suggestions for a Jones/PAMS stain that is not working properly. This is something we don't do often. The last time we did it was a year and a half ago, and it seemed fine at the time. We have tried 3 or 4 protocols, including an ammoniacal silver, and it is still not working properly. In some protocols, our red cells are staining but the capillaries in the glomeruli do not seem to be picking up the silver. In other protocols, there are nuclei of some cells that should not be staining that are, but again, the capillaries are not. We are working on mouse tissue that is fixed in NBF. The strange thing is the stain seems to be working well on Bouins and Telly's fixed tissue. I even tried mordanting in Bouins! We have tried multiple kidneys with the same results. We are on new bottles of silver and periodic acid, although our methenamine has been around a while. Any suggestions would be greatly appreciated. Thanks! Liz From gayle.callis <@t> bresnan.net Fri Mar 2 16:35:33 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Mar 2 16:35:43 2012 Subject: Posting photographs RE: [Histonet] microtomy Message-ID: <000501ccf8c4$c9c52240$5d4f66c0$@bresnan.net> There is a way to post pictures on histonet but just not in the email messaging. The instructions for doing this are found on the Histonet website. Speed does affect the thickness of sections. It pays to turn the flywheel at a steady, slow pace and not NASCAR racing speeds I have sometime observed. Harder paraffin helps for thin sections but one should be able to section at 2 um without difficulty on a properly adjusted microtome, a sharp disposable blade and using any modern day paraffin. Gayle Callis HTL/HT/MT (ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, March 02, 2012 2:54 PM To: Webb, Dorothy L Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microtomy Love your question. Hate to hear that you are having a issue. My two cents follow: Yes. The amount of time for a faced block will effect the section thickness. The cells become bloated if they Sit to long. < feel free to have a Friday laugh on this one. Anyway. If you havnt changed the stain in any way or the tech isn't rushing and hard facing causing extrenuated cell artifact and you don't have bloated cells? Could it be possible his eyesight got better or maybe he is mad and wants to pick a fuss? I sometimes wish we could post pictures if our issues. I'm wishing you the best. Kim D Sent from my iPhone On Mar 2, 2012, at 1:06 PM, "Webb, Dorothy L" wrote: > We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! > > Dorothy Webb, HT (ASCP) > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to > the sender immediately and delete the original message and any copy of > it from your computer system. If you have any questions concerning > this message, please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duraine <@t> bcm.edu Fri Mar 2 17:05:19 2012 From: duraine <@t> bcm.edu (Duraine, Lita) Date: Fri Mar 2 17:05:23 2012 Subject: [Histonet] Multiple stain for epoxy sections Message-ID: <9D2C4E815F57784DAE6914BA54C82324CAEDCC4B50@EXCMSMBX06.ad.bcm.edu> Hello Histonet Users, I have decided that I should ask the experts. I regularly thick section muscle sections in Embed 812 for bouton identification. I normally use Toluidine blue. I am having trouble differentiating between boutons and a type of vacuole that occurs within the same tissue and sometimes same area using TB. Under the LM they can sometimes appear the same. That is until you get it in the TEM and then you have a vacuole and not a bouton. In the Light Microscope the slide under an oil lens doesn't really indicate sub synaptic reticulum other than a darker area. Does anyone know of a multiple stain that can be used for epoxy resin sections (no methanol or Ethanol) that would stain SSR or vesicles a different color from other surrounding areas. I have checked online and indeed there are many stains but from what I read the protocols are for paraffin sections. Surely there is something. Thank you, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics Duncan Neurological Research Institute 1250 Moursund St. Houston, TX 77030 Rm: N1165.17 MS: N1125.50 832-824-8772 TEM Room 979-549-6526 Cell http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php From Marilyn.A.Weiss <@t> kp.org Fri Mar 2 18:01:37 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Mar 2 18:02:01 2012 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 03/02/2012 and will not return until 03/05/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From rosenfeldtek <@t> hotmail.com Fri Mar 2 18:41:33 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Fri Mar 2 18:41:37 2012 Subject: [Histonet] RE: Congo red In-Reply-To: References: Message-ID: >From the CAP site: "The Benhold Congo red technique does not yield reproducible results and should be avoided" and "the alkaline Congo red method of Puchtler, et al.,7 remains the gold standard for the demonstration of amyloid in tissue sections. " http://www.cap.org/apps/portlets/contentViewer/show.do?printFriendly=true&contentReference=cap_today%2F0609%2F0609g_histologic_preparations.html Jerry > From: cmiller@physlab.com > To: histonet@lists.utsouthwestern.edu > CC: histonet-bounces@lists.utsouthwestern.edu > Date: Thu, 1 Mar 2012 08:28:18 -0600 > Subject: [Histonet] Congo red > > Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak. > > Cheryl A. Miller HT(ASCP)cm > Histology/Cytology Prep Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4145 ext. 554 > > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madsen_marie <@t> hotmail.com Sat Mar 3 02:53:07 2012 From: madsen_marie <@t> hotmail.com (Marie Madsen) Date: Sat Mar 3 02:53:18 2012 Subject: [Histonet] Frozen tissue detachment In-Reply-To: References: Message-ID: Hi everyone, We're having trouble with frozen tissue detachment (mouse aortic root). Our procedure is as follows: 1. Fresh heart into NBF 4% (Lilly's) 24h, fridge (+4) 2. OCT (tissue-tek) 2h, fridge (+4) 3. Quickly frozen in icecold isopentan 4. Freezer (-20) until sectioned at 10 ?m in cryostat (at approx -20) 5. RT one to several hours before being put back into the freezer. We use Superfrost glass slides. The *only* thing I can think of is wrong, is that the sections have been thawed/frozen several times before being used for staining (we do that to find the best section for our stainings) -do you think that's the problem? Another thing could be that after being sectioned we don't have a specific amount of time and temperature at which the sections should dry.. I found out recently regarding my IHC protocol, that removing the OCT with 70% EtOH (10 min => dry 20 min) makes the tissue stick better than removing it with dH2O or TBS..but then the specific staining also became much more faded..any ideas what else to do? Sorry about all the questions -I'm relatively new to this field, but find it very interesting. Best, Marie From gu.lang <@t> gmx.at Sat Mar 3 07:39:57 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 3 07:40:03 2012 Subject: AW: [Histonet] microtomy In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Message-ID: I thought it as a rule, that the thickness of slides vary depending on everything (inclusive weather, humidity, person, speed, temperature, cutting angle...). Although not very scientific, only the techs experience and "eye" will fix the problem. As for the duration on ice: If you have "wet" ice, the tissue can take up the humidiy and swell (if too long, the tissue comes beyond the surface), but the slides wouldn't be thicker. The cooler the block, the longer the temperature is held while sectioning, the longer the thickness is stable. But if you give one breath to the surface, the section is possibly double thick. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Freitag, 02. M?rz 2012 19:07 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] microtomy We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Sat Mar 3 07:43:56 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat Mar 3 07:44:02 2012 Subject: AW: [Histonet] microtomy In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Message-ID: <1330782236.36238.YahooMailNeo@web5716.biz.mail.ne1.yahoo.com> Correct Gudrun! Where is the like button? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Gudrun Lang To: "'Webb, Dorothy L'" Cc: histonet@lists.utsouthwestern.edu Sent: Saturday, March 3, 2012 7:39 AM Subject: AW: [Histonet] microtomy I thought it as a rule, that the thickness of slides vary depending on everything (inclusive weather, humidity, person, speed, temperature, cutting angle...). Although not very scientific, only the techs experience and "eye" will fix the problem. As for the duration on ice: If you have "wet" ice, the tissue can take up the humidiy and swell (if too long, the tissue comes beyond the surface), but the slides wouldn't be thicker. The cooler the block, the longer the temperature is held while sectioning, the longer the thickness is stable. But if you give one breath to the surface, the section is possibly double thick. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Freitag, 02. M?rz 2012 19:07 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] microtomy We have run into an interesting scenario and wondering what the "experts" think!? We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome.? Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns.? I had our service technician? measure the microns and the equipment was cutting as set.? I had the blocks cut on a different microtome and we have seen variations there also.? My question is, does the amount of time on ice make a minor difference in the section thickness?? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation!? This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!!? Thank you!! Dorothy Webb, HT (ASCP) ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Sat Mar 3 11:32:17 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Sat Mar 3 11:32:21 2012 Subject: [Histonet] lab. setting up Message-ID: <8835095097320589777@unknownmsgid> Dear Mohamed, Please feel free to email me directly. I will gladly assist you. I am currently opening a facility in California, and would be pleased to share with you the equipment decisions we made, and why. Most equipment is made in Germany, England or Japan. As such, I am sure it is all very available in Egypt. Let me know if I can be of help. Sent from my Windows Phone From: mohamed abd el razik Sent: 3/2/2012 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab. setting up dear all on histonet I'm going to setup a new histology lab.?in Egypt. I need to contact with you to advice me about the best and comfotable facilities microtomes- processors-automatic stainers and so on ..... Mohamed Ass. Lec. of histology Faculty of Vet. Med. Cairo University Egypt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Mar 3 17:10:23 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Mar 3 17:10:43 2012 Subject: AW: [Histonet] microtomy In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Message-ID: <68630C6D-A3AB-427D-9582-0C003EC97871@yahoo.com> We are talking about reality here. Not what it says in some text book written by someone who hasn't cut a block in years. The tech is going to take the first good looking section they get after they have sat on the ice most Likely waiting for the tech who is so busy with 100 other task. So if long sitting bloated blocks is a new practice in your daily routine. Sure. You can get some thick sections tossed in your days work. Happy days. Sent from my iPhone On Mar 3, 2012, at 8:39 AM, "Gudrun Lang" wrote: > I thought it as a rule, that the thickness of slides vary depending on > everything (inclusive weather, humidity, person, speed, temperature, cutting > angle...). > Although not very scientific, only the techs experience and "eye" will fix > the problem. > > As for the duration on ice: If you have "wet" ice, the tissue can take up > the humidiy and swell (if too long, the tissue comes beyond the surface), > but the slides wouldn't be thicker. The cooler the block, the longer the > temperature is held while sectioning, the longer the thickness is stable. > But if you give one breath to the surface, the section is possibly double > thick. > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, > Dorothy L > Gesendet: Freitag, 02. M?rz 2012 19:07 > An: 'histonet@lists.utsouthwestern.edu' > Betreff: [Histonet] microtomy > > We have run into an interesting scenario and wondering what the "experts" > think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on > one particular microtome. Within the past month, the hematopathologist has > felt the sections are thicker than the usual 3 microns. I had our service > technician measure the microns and the equipment was cutting as set. I had > the blocks cut on a different microtome and we have seen variations there > also. My question is, does the amount of time on ice make a minor > difference in the section thickness? I know a lot of responses may be the > difference in the tech cutting inasmuch as how fast they turn the rotations, > etc., but,we have ruled out that variable by having more than one tech cut > at the microtome in question. I am stymied as to how to remedy this > fluctuation! This is why we love histology, so many variables to create a > problem and why I love histonet, so many techs to help one through a > dilemma!! Thank you!! > > Dorothy Webb, HT (ASCP) > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tuyenmai77 <@t> yahoo.com Sun Mar 4 04:21:29 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Sun Mar 4 04:21:33 2012 Subject: [Histonet] Fwd: Message-ID: <1330856489.74534.yint-ygo-j2me@web162805.mail.bf1.yahoo.com> Greetings, friend! http://www.fminformatica.com/like.php?wutyg=44&ocugimuv=215&ivozuvitu=99 From madeleinehuey <@t> gmail.com Sun Mar 4 15:18:24 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sun Mar 4 15:18:30 2012 Subject: [Histonet] Re: Histonet Digest, Vol 100, Issue 3 In-Reply-To: <4f525c4c.09beec0a.2ffb.6731SMTPIN_ADDED@mx.google.com> References: <4f525c4c.09beec0a.2ffb.6731SMTPIN_ADDED@mx.google.com> Message-ID: From: Courtney Pierce Subject: [Histonet] AKT and pAKT Has anyone out there done AKT and pAKT antibodies for IHC. I have been working on them for about a month now and they are still not working right. I hope there is someone out there that can help me??? Courtney, Maybe you should post your IHC protocol (AKT & pAKT) here, and hopefully we can find the problem & help you. I can help you as well, if you send me your protocol & IHC picture. Best! madeleinehuey@elcaminohospital.org From bridget.maryott <@t> ventana.roche.com Mon Mar 5 08:47:27 2012 From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget) Date: Mon Mar 5 08:48:47 2012 Subject: [Histonet] RE: microtomy Message-ID: You might want to check the tolerance of your instrument. If I remember correctly, even a precisely calibrated microtome can still have a range of around +/- 10%. So even when calibrated correctly, cutting at 3 microns and getting a section of 3.3 microns would still be within the manufacturer's specs. -Bridget Maryott, HT (ASCP) Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. Message: 1 Date: Fri, 2 Mar 2012 12:06:43 -0600 From: "Webb, Dorothy L" Subject: [Histonet] microtomy To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!! Dorothy Webb, HT (ASCP) From g.vanhaalem <@t> prosensa.nl Mon Mar 5 09:25:17 2012 From: g.vanhaalem <@t> prosensa.nl (Geert van Haalem) Date: Mon Mar 5 09:25:30 2012 Subject: [Histonet] replacing tween in the secondary hyb.solution Message-ID: <5F4E852313B6BC4B8C9916B69BAB4C1E05906E36@pro-ex02> Hi, I currently did an experiment in which I left out Tween in de hybridisation solution of the secondary antibodies. I only made a solution of two Alexa in PBS. When imaging Bye eye I did not notice any decrease in signal compared to a hyb.solution with Tween. My intention is to replace tween with FBS, but can anybody tell me pro and cons for this idea. With regards, Geert From rjbuesa <@t> yahoo.com Mon Mar 5 09:46:03 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 5 09:46:10 2012 Subject: [Histonet] replacing tween in the secondary hyb.solution In-Reply-To: <5F4E852313B6BC4B8C9916B69BAB4C1E05906E36@pro-ex02> Message-ID: <1330962363.93868.YahooMailClassic@web162105.mail.bf1.yahoo.com> Not a good idea. The effect of Twenn is to facilitate the dispersion over the section of the reagent. If you do not use it the reagents may not reach all the surface and you?may end with an uneven reaction or your results could be inconsistent. A small savings by not using Tween may mean that you could have to repeat the test and spend much more expensive reagents. Again, not a good idea. Ren? J. --- On Mon, 3/5/12, Geert van Haalem wrote: From: Geert van Haalem Subject: [Histonet] replacing tween in the secondary hyb.solution To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 5, 2012, 10:25 AM Hi, I currently did an experiment in which I left out Tween in de hybridisation solution of the secondary antibodies. I only made a solution of two Alexa in PBS. When imaging Bye eye I did not notice any decrease in signal compared to a hyb.solution with Tween. My intention is to replace tween with FBS, but can anybody tell me pro and cons for this idea. With regards, Geert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KCross <@t> cvm.tamu.edu Mon Mar 5 10:24:03 2012 From: KCross <@t> cvm.tamu.edu (Cross, Kelly) Date: Mon Mar 5 10:24:11 2012 Subject: [Histonet] xylene substitute for processing Message-ID: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> Greetings Histonet! Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? Thank you in advance for your help, Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Veterinary Pathobiology Texas Veterinary Medical Center Texas A&M University College Station, TX 77843-4467 979-862-3658 Office 979-845-5149 Lab From collette2 <@t> llnl.gov Mon Mar 5 11:33:38 2012 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Mon Mar 5 11:33:57 2012 Subject: [Histonet] immunofluorescence mounting medium? Message-ID: Hello, Esteemed Histonetters, I am trying to get nice publication-quality images of my immunofluorescent tissue sections. I am currently using Prolong Gold, and after I let the stuff cure for several days, my 100X oil-immersion images are still smeary, even after taking into account the unevenness of the tissue section. I don't seem to have this problem with colorimetric stains (histological stains, LacZ, etc.), so I am thinking the mounting medium is part of the problem? It seems to me that it never fully cures at the middle of the coverslip? Does anyone have a recommendation for a mounting medium that works better for this purpose? Any advice is appreciated. Thanks in advance, and Happy Monday! Sincerely, Nicole Collette Lawrence Livermore National Laboratory collette2@llnl.gov From yujie.wen <@t> louisville.edu Mon Mar 5 11:40:26 2012 From: yujie.wen <@t> louisville.edu (Wen,Yujie) Date: Mon Mar 5 11:40:38 2012 Subject: [Histonet] immunofluorescence mounting medium? In-Reply-To: References: Message-ID: http://www.vectorlabs.com/catalog.aspx?catID=279 VECTASHIELD Mounting Media for Fluorescence This one works for me and was recommended by Leica technical support for oil immersion image. -------- Original Message -------- From: Collette, Nicole M. Sent: Mon, Mar 5, 2012 12:35 PM To: histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] immunofluorescence mounting medium? Hello, Esteemed Histonetters, I am trying to get nice publication-quality images of my immunofluorescent tissue sections. I am currently using Prolong Gold, and after I let the stuff cure for several days, my 100X oil-immersion images are still smeary, even after taking into account the unevenness of the tissue section. I don't seem to have this problem with colorimetric stains (histological stains, LacZ, etc.), so I am thinking the mounting medium is part of the problem? It seems to me that it never fully cures at the middle of the coverslip? Does anyone have a recommendation for a mounting medium that works better for this purpose? Any advice is appreciated. Thanks in advance, and Happy Monday! Sincerely, Nicole Collette Lawrence Livermore National Laboratory collette2@llnl.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Mon Mar 5 12:06:33 2012 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Mar 5 12:06:55 2012 Subject: [Histonet] re: xylene substitute for processing Message-ID: <24B7B291CC88D04AB663958E77A1F59D025CDA@ex09.net.ucsf.edu> We have been processing without xylene on ThermoFisher Excelsior processors for a couple of years. We go directly from 100% isopropyl alcohol into paraffin. A thermo sales rep should be able to give you some literature on this. When we first got the processors we did side by side validation and found no difference in special stains, immuno or H&E. Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 From Lisa.White3 <@t> va.gov Mon Mar 5 12:10:50 2012 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Mon Mar 5 12:11:38 2012 Subject: [Histonet] xylene substitute for processing Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976095E76F9@VHAV09MSGA3.v09.med.va.gov> We use Formula 83 for overnight processing. It works well with no problems for special staining or IHC. The only problem is that the tissues do have to be well fixed before processing. I have also used Americlear (to which I now have an allergy) and Sub-X and had good results with both at other labs from my past. The switch will cost you a small percent of dehydration that xylene will do that substitutes will not. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From vavalos <@t> allergydermatology.com Mon Mar 5 12:21:45 2012 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Mon Mar 5 12:22:00 2012 Subject: [Histonet] RE: xylene substitute for processing In-Reply-To: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> References: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> Message-ID: I use the Shandon Excelsior and use an overnight run with Iso Alcohol and only use Xylene in the cleaning cycle. Our Thermo Rep came out and set it all up for us. No problems with the processing. I also use sub xylene in staining with a few extra steps but no problems. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly Sent: Monday, March 05, 2012 9:24 AM To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] xylene substitute for processing Greetings Histonet! Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? Thank you in advance for your help, Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Veterinary Pathobiology Texas Veterinary Medical Center Texas A&M University College Station, TX 77843-4467 979-862-3658 Office 979-845-5149 Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Mon Mar 5 13:23:16 2012 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Mar 5 12:23:52 2012 Subject: [Histonet] xylene substitute for processing Message-ID: <1186743546.20120305222316@mail.ru> Kelly, We uses for years mineral oil instead xylene for overnight manual processing and have seen not any adverse effect in H&E, HC and IHC. Opposite, we noticed, that our slides becomed better, crisp and clear than with xylene. Mineral oil have also many positive additional moments for techs and lab aides. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From melissa <@t> alliedsearchpartners.com Mon Mar 5 12:49:37 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Mon Mar 5 12:49:48 2012 Subject: [Histonet] Histotech Job Opening in Naples Message-ID: Hello and Happy Monday Histonet, Allied Search Partners has a job opening for a qualified histotech in Naples, FL. This is a permanent (Direct Hire) position for Monday-Friday, Day Shift (6:30am-2:30pm). Below are the requirements. If you are interested and qualified for the position please send me your resume for review. I will in return send you some more details on this position. Have a great day. -Associates Degree required (at least) -2+ years of experience -Florida Clinical Laboratory Licensed -Looking for a long term/permanent position -Interested in relocating to Naples or living in the area I look forward to hearing from you! -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From charchar999 <@t> gmail.com Mon Mar 5 13:33:11 2012 From: charchar999 <@t> gmail.com (Charity Wyatt) Date: Mon Mar 5 13:33:19 2012 Subject: [Histonet] xylene substitute for processing (Cross, Kelly) Message-ID: Hi, Kelly. I used Americlear at one hospital I worked at, and we had no issues with special stains, processing, etc. We even recycled the Americlear and re-used it with good results. Charity Wyatt On Mon, Mar 5, 2012 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 100, Issue 3 (Madeleine Huey) > 2. RE: microtomy (Maryott, Bridget) > 3. replacing tween in the secondary hyb.solution (Geert van Haalem) > 4. Re: replacing tween in the secondary hyb.solution (Rene J Buesa) > 5. xylene substitute for processing (Cross, Kelly) > 6. immunofluorescence mounting medium? (Collette, Nicole M.) > 7. RE: immunofluorescence mounting medium? (Wen,Yujie) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 4 Mar 2012 13:18:24 -0800 > From: Madeleine Huey > Subject: [Histonet] Re: Histonet Digest, Vol 100, Issue 3 > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > From: Courtney Pierce > Subject: [Histonet] AKT and pAKT > > Has anyone out there done AKT and pAKT antibodies for IHC. I have been > working on them for about a month now and they are still not working > right. I hope there is someone out there that can help me??? > > Courtney, > > Maybe you should post your IHC protocol (AKT & pAKT) here, and > hopefully we can find the problem & help you. > > I can help you as well, if you send me your protocol & IHC picture. > > Best! > madeleinehuey@elcaminohospital.org > > > > ------------------------------ > > Message: 2 > Date: Mon, 5 Mar 2012 09:47:27 -0500 > From: "Maryott, Bridget" > Subject: [Histonet] RE: microtomy > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > F66E3A8D37574042826ACCBFB00C5C2102C73545A8@RNUMSEM722.nala.roche.com> > Content-Type: text/plain; charset="us-ascii" > > You might want to check the tolerance of your instrument. If I remember > correctly, even a precisely calibrated microtome can still have a range of > around +/- 10%. So even when calibrated correctly, cutting at 3 microns and > getting a section of 3.3 microns would still be within the manufacturer's > specs. > > -Bridget Maryott, HT (ASCP) > > Confidentiality Note: This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > > > > Message: 1 > Date: Fri, 2 Mar 2012 12:06:43 -0600 > From: "Webb, Dorothy L" > Subject: [Histonet] microtomy > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > < > 65365F35C0F2EF4D846EC3CA73E49C43016BE17354EC@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > We have run into an interesting scenario and wondering what the "experts" > think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on > one particular microtome. Within the past month, the hematopathologist has > felt the sections are thicker than the usual 3 microns. I had our service > technician measure the microns and the equipment was cutting as set. I > had the blocks cut on a different microtome and we have seen variations > there also. My question is, does the amount of time on ice make a minor > difference in the section thickness? I know a lot of responses may be the > difference in the tech cutting inasmuch as how fast they turn the > rotations, etc., but,we have ruled out that variable by having more than > one tech cut at the microtome in question. I am stymied as to how to remedy > this fluctuation! This is why we love histology, so many variables to > create a problem and why I love histonet, so many techs to help one through > a dilemma!! Thank you!! > > Dorothy Webb, HT (ASCP) > > > > > > > ------------------------------ > > Message: 3 > Date: Mon, 5 Mar 2012 15:25:17 +0000 > From: Geert van Haalem > Subject: [Histonet] replacing tween in the secondary hyb.solution > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <5F4E852313B6BC4B8C9916B69BAB4C1E05906E36@pro-ex02> > Content-Type: text/plain; charset="us-ascii" > > Hi, > > I currently did an experiment in which I left out Tween in de > hybridisation solution of > the secondary antibodies. I only made a solution of two Alexa in PBS. When > imaging > Bye eye I did not notice any decrease in signal compared to a hyb.solution > with Tween. > My intention is to replace tween with FBS, but can anybody tell me pro and > cons for > this idea. > > With regards, > > Geert > > > ------------------------------ > > Message: 4 > Date: Mon, 5 Mar 2012 07:46:03 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] replacing tween in the secondary hyb.solution > To: "histonet@lists.utsouthwestern.edu" > , Geert van Haalem > > Message-ID: > <1330962363.93868.YahooMailClassic@web162105.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Not a good idea. The effect of Twenn is to facilitate the dispersion over > the section of the reagent. If you do not use it the reagents may not reach > all the surface and you may end with an uneven reaction or your results > could be inconsistent. A small savings by not using Tween may mean that you > could have to repeat the test and spend much more expensive reagents. > Again, not a good idea. > Ren? J. > > --- On Mon, 3/5/12, Geert van Haalem wrote: > > > From: Geert van Haalem > Subject: [Histonet] replacing tween in the secondary hyb.solution > To: "histonet@lists.utsouthwestern.edu" > > Date: Monday, March 5, 2012, 10:25 AM > > > Hi, > > I currently did an experiment in which I left out Tween in de > hybridisation solution of > the secondary antibodies. I only made a solution of two Alexa in PBS. When > imaging > Bye eye I did not notice any decrease in signal compared to a hyb.solution > with Tween. > My intention is to replace tween with FBS, but can anybody tell me pro and > cons for > this idea. > > With regards, > > Geert > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Mon, 5 Mar 2012 16:24:03 +0000 > From: "Cross, Kelly" > Subject: [Histonet] xylene substitute for processing > To: "Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)" > > Message-ID: > <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> > Content-Type: text/plain; charset="us-ascii" > > Greetings Histonet! > > Does anyone use xylene substitutes for routine over-night processing? If > so, what do you use and does it have any adverse effect your special stains? > > Thank you in advance for your help, > Kelly > > Kelly S. Cross B.S., HT (ASCP) > Medical Laboratory Supervisor > Veterinary Pathobiology > Texas Veterinary Medical Center > Texas A&M University > College Station, TX 77843-4467 > 979-862-3658 Office > 979-845-5149 Lab > > > > ------------------------------ > > Message: 6 > Date: Mon, 5 Mar 2012 09:33:38 -0800 > From: "Collette, Nicole M." > Subject: [Histonet] immunofluorescence mounting medium? > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > Hello, Esteemed Histonetters, > > I am trying to get nice publication-quality images of my immunofluorescent > tissue sections. I am currently using Prolong Gold, and after I let the > stuff cure for several days, my 100X oil-immersion images are still smeary, > even after taking into account the unevenness of the tissue section. I > don't seem to have this problem with colorimetric stains (histological > stains, LacZ, etc.), so I am thinking the mounting medium is part of the > problem? It seems to me that it never fully cures at the middle of the > coverslip? Does anyone have a recommendation for a mounting medium that > works better for this purpose? Any advice is appreciated. > > Thanks in advance, and Happy Monday! > > Sincerely, > Nicole Collette > Lawrence Livermore National Laboratory > collette2@llnl.gov > > > ------------------------------ > > Message: 7 > Date: Mon, 5 Mar 2012 17:40:26 +0000 > From: "Wen,Yujie" > Subject: RE: [Histonet] immunofluorescence mounting medium? > To: "Collette, Nicole M." , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > http://www.vectorlabs.com/catalog.aspx?catID=279 > > VECTASHIELD Mounting Media for Fluorescence > > This one works for me and was recommended by Leica technical support for > oil immersion image. > > -------- Original Message -------- > From: Collette, Nicole M. > Sent: Mon, Mar 5, 2012 12:35 PM > To: histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] immunofluorescence mounting medium? > > > Hello, Esteemed Histonetters, > > I am trying to get nice publication-quality images of my immunofluorescent > tissue sections. I am currently using Prolong Gold, and after I let the > stuff cure for several days, my 100X oil-immersion images are still smeary, > even after taking into account the unevenness of the tissue section. I > don't seem to have this problem with colorimetric stains (histological > stains, LacZ, etc.), so I am thinking the mounting medium is part of the > problem? It seems to me that it never fully cures at the middle of the > coverslip? Does anyone have a recommendation for a mounting medium that > works better for this purpose? Any advice is appreciated. > > Thanks in advance, and Happy Monday! > > Sincerely, > Nicole Collette > Lawrence Livermore National Laboratory > collette2@llnl.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 100, Issue 5 > **************************************** > -- Charity Wyatt Mohs Histotechnologist Jacksonville Skin Cancer Center, P.A./ Michael E. Lutz, M.D. (904)737-0111 From virginiachladek <@t> wpuafl.com Mon Mar 5 14:39:28 2012 From: virginiachladek <@t> wpuafl.com (Virginia Chladek) Date: Mon Mar 5 14:41:08 2012 Subject: [Histonet] Destain/Restain References: Message-ID: <9BB0A278800B0E41BBC28883FFAB45357DE363@exchsvr.wpu.local> **************************************** I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie From kim.tournear <@t> yahoo.com Mon Mar 5 14:52:18 2012 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Mon Mar 5 14:52:22 2012 Subject: [Histonet] histology programs in Okalahoma Message-ID: <1330980738.90192.YahooMailNeo@web120205.mail.ne1.yahoo.com> Hi everyone, Does anyone know of any histology training/programs in Oklahoma? Thanks in advance for all responses. ~Kim~? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ From JMitchell <@t> uwhealth.org Mon Mar 5 16:27:54 2012 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Mon Mar 5 16:27:57 2012 Subject: [Histonet] 2012 Tri-State Symposium Message-ID: Dear Histonetters: You are invited to join the histology societies of Wisconsin, Iowa and Minnesota as they host "An Ocean of Knowledge" at the 2012 Tri-State Symposium, May 2-4 at The Concourse Hotel in Madison, Wisconsin. For program, registration and vendor/exhibit information contact the following representatives: Wisconsin: Jean Mitchell (jmitchell@uwhealth.org) Iowa: Judi Stasko (judith.stasko@ars.usda.gov) Minnesota: Lois Rowe (rowe.lois@mayo.edu) Vendor/Exhibit: Dawn Schneider (dawn.schneider@ministryhealth.org) From kjgada <@t> gmail.com Mon Mar 5 18:43:44 2012 From: kjgada <@t> gmail.com (Komal Gada) Date: Mon Mar 5 18:43:47 2012 Subject: [Histonet] Question about slides Message-ID: Hello Histo-netters, I'm looking for a source which sells slides for a Tissue Identification class. Does anyone have any leads on where to go for something like this? Thanks, Komal From tuyenmai77 <@t> yahoo.com Mon Mar 5 18:45:35 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Mon Mar 5 18:45:38 2012 Subject: [Histonet] Greetings, friend! Message-ID: <1330994735.93267.yint-ygo-j2me@web162803.mail.bf1.yahoo.com> http://extendiendomanos.org/like.php?ficosyzycuge=38&qiqeli=625&jrexesofy=78 From we3smitty <@t> yahoo.com Mon Mar 5 19:32:32 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Mon Mar 5 19:32:35 2012 Subject: [Histonet] glacial acetic acid vs bouins Message-ID: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this.? Does anyone out there remember what dilution works best?? Thanks From Jonathan.Cremer <@t> med.kuleuven.be Tue Mar 6 01:49:16 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Tue Mar 6 01:49:31 2012 Subject: [Histonet] glacial acetic acid vs bouins In-Reply-To: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> References: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> Message-ID: Just an acid wash then, as in the wash after the methyl blue in an MSB or Mallory's trichrome? I have 1% and 0.5% for those stains, respectively. My guess would be that the concentration of acetic acid is not that important, just the fact that it lowers the pH of the water so specific stain does not wash out of the section. No idea what stain you're doing though. Might be something else entirely. Best --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens angela smith [we3smitty@yahoo.com] Verzonden: dinsdag 6 maart 2012 2:32 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] glacial acetic acid vs bouins I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy.Giroux <@t> stjohn.org Tue Mar 6 06:35:33 2012 From: Stacy.Giroux <@t> stjohn.org (Giroux, Stacy) Date: Tue Mar 6 06:35:39 2012 Subject: [Histonet] Smad4 (B-8) / DPC4 Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3E0709AE9@AUSP03VMBX11.apptixhealth.net> Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital & Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From cgill <@t> marylandgeneral.org Tue Mar 6 07:31:52 2012 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Tue Mar 6 07:32:04 2012 Subject: [Histonet] glacial acetic acid vs bouins In-Reply-To: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> References: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23AA011@MDGEN-EXCH1.marylandgeneral.org> What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well. Hope this helps . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, March 05, 2012 8:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glacial acetic acid vs bouins I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Mar 6 07:34:00 2012 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Mar 6 07:34:12 2012 Subject: [Histonet] Smad4 (B-8) / DPC4 In-Reply-To: <29CCF3EC44815745864D9F84A1ED4B51B3E0709AE9@AUSP03VMBX11.apptixhealth.net> References: <29CCF3EC44815745864D9F84A1ED4B51B3E0709AE9@AUSP03VMBX11.apptixhealth.net> Message-ID: <4F55CBF8.2B7F.00C9.1@geisinger.edu> I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know. Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Giroux, Stacy" 3/6/2012 7:35 AM >>> Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital & Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From cgill <@t> marylandgeneral.org Tue Mar 6 07:34:25 2012 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Tue Mar 6 07:34:37 2012 Subject: [Histonet] glacial acetic acid vs bouins In-Reply-To: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> References: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23AA014@MDGEN-EXCH1.marylandgeneral.org> What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well. Hope this helps . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, March 05, 2012 8:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glacial acetic acid vs bouins I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakerj <@t> umich.edu Tue Mar 6 07:46:49 2012 From: bakerj <@t> umich.edu (John Baker) Date: Tue Mar 6 07:46:58 2012 Subject: [Histonet] tissue processors Message-ID: <6274951A-A955-43AF-A745-9CD70ED76F23@umich.edu> Hello Histoworld, What processors are people using to process bone or soft tissue samples for plastic? Do any of your processors allow for use of the monomer in the processor before embedding? What are your opinions, pro or cons, on the Tissue- Tek VIP5 or 6, the Leica ASP300, or the Thermo Pathcentre units? Hearing yours experiences in using them is greatly appreciated! My best, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From Luis.Chiriboga <@t> nyumc.org Tue Mar 6 08:01:49 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Tue Mar 6 08:02:43 2012 Subject: [Histonet] IHC + control question Message-ID: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis From jshelley <@t> sanfordburnham.org Tue Mar 6 08:04:10 2012 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Mar 6 08:04:33 2012 Subject: [Histonet] CD11c and CD206 Message-ID: <5A605CE38EECB64B94485C02125A0C441AB810B061@LN-MAIL07.ln.burnham.org> Hi All, Was wondering if anyone has had experince using these antibodies. I have been given these antibodies from an investigator and they are from Ab Serotec. One is Rabbit and the other is Armenian Hamster it appears I can get the CD11c to work with a mild protease but the CD 206 does not appear to work under all the conditions I have tried. Too many to list but If someone has worked with both or one of these antibodies your help would be greatly appreciated. Kind regards! John Shelley From sbreeden <@t> nmda.nmsu.edu Tue Mar 6 08:42:54 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 6 08:43:12 2012 Subject: [Histonet] Control Block Tracking Message-ID: <02C099024072804EA34F5906BAC30A413F1F5A@nmdamailsvr.nmda.ad.nmsu.edu> During a recent inspection of my department, the inspector "encouraged" me to develop a method of tracking which control tissue I used for a case (i.e., individually identifying the block and referencing that identifier on the slide). To be honest, I believe this to be overload, but since I'm probably going to have to abide by that "encouragement", I'd like to know if/how you identify which control block is used for a special stain. I can think of a simple method but wonder if any of you have used a really spectacular method that just blew the inspecting agency away. I'd promptly plagiarize it! Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From TMcNemar <@t> lmhealth.org Tue Mar 6 08:57:22 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Mar 6 08:57:07 2012 Subject: [Histonet] RE: Destain/Restain In-Reply-To: <9BB0A278800B0E41BBC28883FFAB45357DE363@exchsvr.wpu.local> References: <9BB0A278800B0E41BBC28883FFAB45357DE363@exchsvr.wpu.local> Message-ID: We do not destain h&e slides. We just take them back to water and stain right over top of the h&e. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Virginia Chladek Sent: Monday, March 05, 2012 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destain/Restain **************************************** I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From akbitting <@t> geisinger.edu Tue Mar 6 09:04:12 2012 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Mar 6 09:04:31 2012 Subject: [Histonet] IHC + control question In-Reply-To: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Message-ID: <4F55E11D.2B7F.00C9.1@geisinger.edu> My vote has always been for vimentin. See Dako's booklet IHC Staining Methods, 5th Ed, Chapter 19 addresses this. Angie >>> "Chiriboga, Luis" 3/6/2012 9:01 AM >>> Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From LSebree <@t> uwhealth.org Tue Mar 6 09:13:06 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 6 09:13:14 2012 Subject: [Histonet] IHC + control question In-Reply-To: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Message-ID: Vimentin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, March 06, 2012 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC + control question Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Mar 6 09:26:01 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Mar 6 09:26:08 2012 Subject: [Histonet] Re: glacial acetic acid vs bouins Message-ID: On the subject of "fixing" marking inks (india ink, Davidson inks and what have you) by dipping the specimen into a "fixing" agent: I agree that Bouin's fixative shouldn't be used, as much because of its messiness as its toxicity and possible explosion hazard. I've worked in labs that used acetone, an unacceptable fire hazard. 2% acetic acid (roughly half-strength white vinegar) is supposed to work as well as anything. I've been grossing since before a fair number of you were born, and I don't use anything. The important thing is to blot the specimen thoroughly dry before inking it, whether it's unfixed or formalin fixed. And realizing that nothing will stick to a cauterized surface (such as a LEEP surgical specimen), but the microscopist can see the cauterized surfaces without any inking. Bob Richmond Samurai Pathologist Knoxville TN From rjbuesa <@t> yahoo.com Tue Mar 6 10:32:06 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 6 10:32:14 2012 Subject: [Histonet] Control Block Tracking In-Reply-To: <02C099024072804EA34F5906BAC30A413F1F5A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1331051526.19102.YahooMailClassic@web162103.mail.bf1.yahoo.com> Absolutely unnecessary! You have to remember that an "inspector", in order to appear knowledgeable and "demanding", has to say something, even if it is stupid. But you can comply in a simple way (as I used to do):? make a list of your control blocks, with documentation about what antigens?they are?used for. Also write the dates from/to that particular block was used. If you want to know what control was used for a specific case/antigen, just check the data the test was performed, and refer to your control blocks list to know which was use according with the date. The problem arises when you use, for example normal?tonsils, although those can also be referred to a case number (the one corresponding to the tonsil resection). In this way you will comply without the need to add additional information to every case. Ren? J. --- On Tue, 3/6/12, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Control Block Tracking To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 6, 2012, 9:42 AM During a recent inspection of my department, the inspector "encouraged" me to develop a method of tracking which control tissue I used for a case (i.e., individually identifying the block and referencing that identifier on the slide).? To be honest, I believe this to be overload, but since I'm probably going to have to abide by that "encouragement", I'd like to know if/how you identify which control block is used for a special stain.? I can think of a simple method but wonder if any of you have used a really spectacular method that just blew the inspecting agency away.? I'd promptly plagiarize it!? Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 6 10:36:21 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 6 10:36:29 2012 Subject: [Histonet] IHC + control question In-Reply-To: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Message-ID: <1331051781.48063.YahooMailClassic@web162105.mail.bf1.yahoo.com> Vimentin Williams et al.(1997); J.Clin.Pathol.,50:422-8 Ren? J. --- On Tue, 3/6/12, Chiriboga, Luis wrote: From: Chiriboga, Luis Subject: [Histonet] IHC + control question To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 6, 2012, 9:01 AM Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From admin <@t> fullstaff.org Tue Mar 6 10:43:09 2012 From: admin <@t> fullstaff.org (Cheryl Kerry) Date: Tue Mar 6 10:43:15 2012 Subject: [Histonet] Open positions...Oh MY! Message-ID: Hi Guys- I don't post often for job openings as usually our network of people help us find good techs for the labs we serve. FORTUNATELY it's getting really busy...feels like the economy isn't quite as icky as our list is getting ever longer and we need help finding GOOD people! Temp or permanent--new grad or old school--we're here to help. The referral-a-friend bonus program is still in place--let us know if you have a friend looking... Here's the short list--if you don't see what you want call, we have more than those listings: URGENT TEMP NEED: Traveling PA - ASCP certified with two years paid experience--call and we'll fill you in on the opportunity. PERMANENT OPENINGS (direct W-2 hire): 1. Pennsylvania: A. Private Small Reference Lab: 2 HISTOTECHS, 1 PA/GROSSER: A newly remodeled private lab has two day shift openings for full service including specials and IHC. Two Histotechs to work together as well as a possible PA or Grosser to fill out the team. They have an aid and it's one of those jobs where you do it all and get the kudos you deserve. An 'old-school' lab and one worth exploring if you're looking for a long-term career opportunity. GREAT DOCS! B. Large reference facility: 1 HISTOTECH: A routine Histotech position. Good pay and a possible career path with a large private company C. Large reference facility: 1 HISTOTECH: Routine histology near one of the nicest cities in which to raise a family (my hometown!) 2. Florida Gulf Coast Hospital: 1 SUPERVISOR and 2 HISTOTECHS All day-shift for a private community hospital rebuilding their team. IF YOU DON"T HAVE A FLORIDA LICENSE--CALL ANYWAY. An opportunity to move into a facility that cares enough to stand up for their techs and get them the pay and the environment to do the best work for their patients! I like the lab manager--she had a great plan for this group! 3. Ohio Private Hospital Facility: 3 HISTOTECHS Three techs. One handling the autopsy suite and some histology, the other two will fill out the Histology team. They are rebuilding their team with an eye toward making it the best place in the area to work. 4. Texas A. Private medium-sized reference lab: 1 LEAD, 1 GROSSER, 2 HISTOTECHS Multiple positions including a lead that might also serve as lead grosser, a grossing tech and several Histotech positions with several start times. They have been a great place to work and with new management, are back on track. B. Private small reference lab: 1 HISTOTECH and possibly 1 IHC TECH Lovely newly remodeled private reference facility outside a major city. Nice management, routine work and a good pay rate...nice place to raise a family, too. C. Large reference lab: PA or QUALIFIED/EXPERIENCED GROSSING HISTOTECH They aren't quite sure of the shift (evenings or early AM) but this is a career facility with promotion from within. Very nice facilities, good pay and benies. 5 Colorado Private medium reference facility: 2 HISTOTECHS A NICE PLACE TO WORK....our temps convert to permanent at this place! Can't get a better compliment than that. They're growing and need more qualified help. They do work with students and train but new blood brings in new ideas. I've benched with this supervisor--she's a straight shooter--easy as you know what is expected and how you're doing. Good pay--BEAUTIFUL city. 6. HISTOTECHS & PATHOLOGIST'S ASSISTANTS: Seeking multiple PAs for a large number of temp and permanent openings...there just aren't enough of you guys. As always--we invite the curious to just pick up the phone and ask all your questions--worst case you spend a few minutes. Best case you find a job you LOVE! Thanks-- Cheryl Cheryl Kerry, HT(ASCP), Coordinator Full Staff Inc. www.fullstaff.org 281.852.9457 Phone 800.756.3309 Fax and Phone admin@fullstaff.org Follow us on our website & blog: www.fullstaff.org or through Facebook: http://www.facebook.com/TheHistologyCompany From campbellj <@t> muhlbauerlab.com Tue Mar 6 10:47:45 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Tue Mar 6 10:47:54 2012 Subject: [Histonet] Question about slides In-Reply-To: References: Message-ID: Ward's Natural Science. On Mon, Mar 5, 2012 at 7:43 PM, Komal Gada wrote: > Hello Histo-netters, > > I'm looking for a source which sells slides for a Tissue Identification > class. Does anyone have any leads on where to go for something like this? > > Thanks, > Komal > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From Diane.Mielnikowski <@t> leica-microsystems.com Tue Mar 6 10:00:46 2012 From: Diane.Mielnikowski <@t> leica-microsystems.com (Diane.Mielnikowski@leica-microsystems.com) Date: Tue Mar 6 11:37:13 2012 Subject: [Histonet] AUTO: Mielnikowski, Diane is out of the office. (returning 03/09/2012) Message-ID: I am out of the office until 03/09/2012. I am in training all week with limited access to email or voicemail. I will return your message as soon as possible. If this is an emergency, please text my cell at 847-257-3111. Diane Note: This is an automated response to your message "Histonet Digest, Vol 100, Issue 6" sent on 3/6/2012 9:01:50 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From TGoins <@t> mt.gov Tue Mar 6 11:59:48 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Mar 6 12:00:09 2012 Subject: [Histonet] Control Block Tracking In-Reply-To: <1331051526.19102.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <02C099024072804EA34F5906BAC30A413F1F5A@nmdamailsvr.nmda.ad.nmsu.edu> <1331051526.19102.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: I must disagree - at one time we also kept a control block list - it can very easily become a record keeping nightmare. We write the ID of the control tissue used in the data base and on the slide label - end of story. No control block written records to update, file, access or archive. My four cents, Tresa Goins Montana Veterinary Diagnostic Lab Bozeman, Montana 59718 406-994-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 06, 2012 9:32 AM To: histonet@lists.utsouthwestern.edu; SaraBreeden Subject: Re: [Histonet] Control Block Tracking Absolutely unnecessary! You have to remember that an "inspector", in order to appear knowledgeable and "demanding", has to say something, even if it is stupid. But you can comply in a simple way (as I used to do):? make a list of your control blocks, with documentation about what antigens?they are?used for. Also write the dates from/to that particular block was used. If you want to know what control was used for a specific case/antigen, just check the data the test was performed, and refer to your control blocks list to know which was use according with the date. The problem arises when you use, for example normal?tonsils, although those can also be referred to a case number (the one corresponding to the tonsil resection). In this way you will comply without the need to add additional information to every case. Ren? J. --- On Tue, 3/6/12, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Control Block Tracking To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 6, 2012, 9:42 AM During a recent inspection of my department, the inspector "encouraged" me to develop a method of tracking which control tissue I used for a case (i.e., individually identifying the block and referencing that identifier on the slide).? To be honest, I believe this to be overload, but since I'm probably going to have to abide by that "encouragement", I'd like to know if/how you identify which control block is used for a special stain.? I can think of a simple method but wonder if any of you have used a really spectacular method that just blew the inspecting agency away.? I'd promptly plagiarize it!? Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> pa.gov Tue Mar 6 12:09:58 2012 From: rcharles <@t> pa.gov (Charles, Roger) Date: Tue Mar 6 12:10:08 2012 Subject: [Histonet] Control Block Tracking In-Reply-To: References: <02C099024072804EA34F5906BAC30A413F1F5A@nmdamailsvr.nmda.ad.nmsu.edu> <1331051526.19102.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: <3809C163DC1DA54AA534B3C7794D07B6AC454BBFF4@ENHBGMBX01.PA.LCL> I also like to use our LIMS system as a tracking tool. In house controls have an accession number that will be on all control blocks and any out of house controls I get are accessioned into LIMS thus giving me electronic records and a case number to use on the control block. Traceability is an AAVLD regulation. Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, March 06, 2012 1:00 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; SaraBreeden Subject: RE: [Histonet] Control Block Tracking I must disagree - at one time we also kept a control block list - it can very easily become a record keeping nightmare. We write the ID of the control tissue used in the data base and on the slide label - end of story. No control block written records to update, file, access or archive. My four cents, Tresa Goins Montana Veterinary Diagnostic Lab Bozeman, Montana 59718 406-994-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 06, 2012 9:32 AM To: histonet@lists.utsouthwestern.edu; SaraBreeden Subject: Re: [Histonet] Control Block Tracking Absolutely unnecessary! You have to remember that an "inspector", in order to appear knowledgeable and "demanding", has to say something, even if it is stupid. But you can comply in a simple way (as I used to do):? make a list of your control blocks, with documentation about what antigens?they are?used for. Also write the dates from/to that particular block was used. If you want to know what control was used for a specific case/antigen, just check the data the test was performed, and refer to your control blocks list to know which was use according with the date. The problem arises when you use, for example normal?tonsils, although those can also be referred to a case number (the one corresponding to the tonsil resection). In this way you will comply without the need to add additional information to every case. Ren? J. --- On Tue, 3/6/12, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Control Block Tracking To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 6, 2012, 9:42 AM During a recent inspection of my department, the inspector "encouraged" me to develop a method of tracking which control tissue I used for a case (i.e., individually identifying the block and referencing that identifier on the slide).? To be honest, I believe this to be overload, but since I'm probably going to have to abide by that "encouragement", I'd like to know if/how you identify which control block is used for a special stain.? I can think of a simple method but wonder if any of you have used a really spectacular method that just blew the inspecting agency away.? I'd promptly plagiarize it!? Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Mar 6 12:13:23 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 6 12:13:37 2012 Subject: [Histonet] IHC + control question In-Reply-To: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Message-ID: <4F560D73.7400.0077.1@harthosp.org> I know that many labs run "Vimentin" for this, but please give consideration to running a nuclear marker as well especially since some of our predictive markers (ER and PR) are nuclear proteins. In my opinion, Vimentin is very "robust" and may show-up even when the tissue is not adequately fixed and/or processed. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Chiriboga, Luis" 3/6/2012 9:01 AM >>> Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCBRITTON <@t> Cheshire-Med.COM Tue Mar 6 12:19:06 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Tue Mar 6 12:19:16 2012 Subject: [Histonet] Destain/Restain In-Reply-To: <9BB0A278800B0E41BBC28883FFAB45357DE363@exchsvr.wpu.local> References: <9BB0A278800B0E41BBC28883FFAB45357DE363@exchsvr.wpu.local> Message-ID: We don't destain them! We remove the coverslip and bring them down to water again with the controls you will be running. We then run them on the Bond with out the Bake and De-wax step. The stain gets removed mostly when you bring them to water again. Our Pathologists have not complained when we do this! Good Luck, Josie Britton HT Cheshire Medical Center Keene, NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Virginia Chladek Sent: Monday, March 05, 2012 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destain/Restain **************************************** I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Tue Mar 6 12:43:04 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Mar 6 12:43:13 2012 Subject: [Histonet] Smad4 (B-8) / DPC4 In-Reply-To: <4F55CBF8.2B7F.00C9.1@geisinger.edu> References: <29CCF3EC44815745864D9F84A1ED4B51B3E0709AE9@AUSP03VMBX11.apptixhealth.net> <4F55CBF8.2B7F.00C9.1@geisinger.edu> Message-ID: Our SMAD-4 is run on the Biocare Intellipath using the Santa Cruz B-8 clone. We do Diva retrieval, 1:200 dilution for 30 minutes followed by PeroxAbolish to eliminate non-specific background staining. Our pathology group has been happy with this. On Tue, Mar 6, 2012 at 5:34 AM, Angela Bitting wrote: > I've tried to work it up on the XT also with inconsistent, weak results. > If you get a reply on this one, let me know. > > Angie > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > > >>> "Giroux, Stacy" 3/6/2012 7:35 AM >>> > Hi, > > I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are > currently trying to work up this antibody for validation; however, we are > having significant problems getting it to work consistently. We are using > the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If > anyone has protocol information to share regardless of platform used it > would be greatly appreciated. > > Thanks, > Stacy > > > Stacy Giroux, HTL(ASCP) > Operations Coordinator, Histology > St. John Hospital & Medical Center > Phone: 313-343-3130 > Fax: 313-343-4965 > > Histotechnology Professionals Day - March 10, 2012 > > > CONFIDENTIALITY NOTICE: > This email message and any accompanying data or files is confidential and > may contain privileged information intended only for the named > recipient(s). If you are not the intended recipient(s), you are hereby > notified that the dissemination, distribution, and or copying of this > message is strictly prohibited. If you receive this message in error, or > are not the named recipient(s), please notify the sender at the email > address above, delete this email from your computer, and destroy any copies > in any form immediately. Receipt by anyone other than the named > recipient(s) is not a waiver of any attorney-client, work product, or other > applicable privilege._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It > is intended solely for the addressee. Access to this message by anyone else > is unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From Courtney.Pierce <@t> quintiles.com Tue Mar 6 13:19:51 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Tue Mar 6 13:20:04 2012 Subject: [Histonet] Microtome Message-ID: Do anyone have a HM 325 Microtome for sale. I'm looking for one. Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From srishan <@t> mail.holyname.org Tue Mar 6 13:30:47 2012 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue Mar 6 13:31:24 2012 Subject: [Histonet] Bradley's single cassette labeller Message-ID: Has anyone used this single cassette labeler? (by Bradley) If so could you please contact me Thanks Nirmala Srishan 201 833 3023 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From tracykme <@t> comcast.net Tue Mar 6 13:31:29 2012 From: tracykme <@t> comcast.net (Tracy) Date: Tue Mar 6 13:31:39 2012 Subject: [Histonet] Online HTL Message-ID: <46F39DB1-6B39-41D9-A028-08AEBBE41D14@comcast.net> Does anyone know of an online HTL program? Thanks From madeleinehuey <@t> gmail.com Tue Mar 6 14:22:33 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Tue Mar 6 14:22:41 2012 Subject: [Histonet] Re: Histonet Digest, Vol 100, Issue 6 In-Reply-To: <4f5627d0.056be00a.7c31.fffffe3eSMTPIN_ADDED@mx.google.com> References: <4f5627d0.056be00a.7c31.fffffe3eSMTPIN_ADDED@mx.google.com> Message-ID: Maggie, What type of slide you have the H&E staining? Charged or uncharged? Tissue section will most likely fall off after the antigen retrieval. Try this; 1) Remove cover slip from H&E slide 2) Hydrate your tissue slide 3) Bake H&E slide overnight in the oven @ 60c - 65c (over the weekend will be better; Not recommend for ER/PR, & HER2) 4) Load to Leica Bond with ER2 for 20 min & (or your usual protocol) This should work because I have done it many times in different labs (you might lost some tissues, but most of them should stay on the slide (manufacture dependant). Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org From: Jonathan Cremer From: "Virginia Chladek" Subject: [Histonet] Destain/Restain I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie From sbreeden <@t> nmda.nmsu.edu Tue Mar 6 14:24:29 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 6 14:24:51 2012 Subject: [Histonet] Control Block Tracking, Part 2 Message-ID: <02C099024072804EA34F5906BAC30A413F1F6E@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to everyone for your tracking suggestions. I think we all agree that there are reasonable regulations and requirements for QA, and we can also agree that some are doing nothing but making our jobs more complex when it's really unnecessary. Soon, every laboratory professional will be attached to a tech robot - your clone physically! Every time you make a move, the "docutech" photographs and documents. At the end of the work day, you unplug your "docutech" and put him/her/it in your locker for recharging. I'm thinkin' there must be a movie in here somewhere - and it may not be a sci-fi feature. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From lblazek <@t> digestivespecialists.com Tue Mar 6 14:30:49 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Mar 6 14:30:54 2012 Subject: [Histonet] RE: Control Block Tracking, Part 2 In-Reply-To: <02C099024072804EA34F5906BAC30A413F1F6E@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F1F6E@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA021B0@IBMB7Exchange.digestivespecialists.com> And the count down continues......... 18 days -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 06, 2012 3:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Block Tracking, Part 2 Thank you to everyone for your tracking suggestions. I think we all agree that there are reasonable regulations and requirements for QA, and we can also agree that some are doing nothing but making our jobs more complex when it's really unnecessary. Soon, every laboratory professional will be attached to a tech robot - your clone physically! Every time you make a move, the "docutech" photographs and documents. At the end of the work day, you unplug your "docutech" and put him/her/it in your locker for recharging. I'm thinkin' there must be a movie in here somewhere - and it may not be a sci-fi feature. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Mar 6 15:17:25 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Mar 6 15:17:32 2012 Subject: [Histonet] RE: Control Block Tracking, Part 2 In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39137DA021B0@IBMB7Exchange.digestivespecialists.com> References: <02C099024072804EA34F5906BAC30A413F1F6E@nmdamailsvr.nmda.ad.nmsu.edu> <5A2BD13465E061429D6455C8D6B40E39137DA021B0@IBMB7Exchange.digestivespecialists.com> Message-ID: <25A4DE08332B19499904459F00AAACB719B2692A53@EVS1.archildrens.org> Lucky lady.... Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, March 06, 2012 2:31 PM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Control Block Tracking, Part 2 And the count down continues......... 18 days -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 06, 2012 3:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Block Tracking, Part 2 Thank you to everyone for your tracking suggestions. I think we all agree that there are reasonable regulations and requirements for QA, and we can also agree that some are doing nothing but making our jobs more complex when it's really unnecessary. Soon, every laboratory professional will be attached to a tech robot - your clone physically! Every time you make a move, the "docutech" photographs and documents. At the end of the work day, you unplug your "docutech" and put him/her/it in your locker for recharging. I'm thinkin' there must be a movie in here somewhere - and it may not be a sci-fi feature. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From g.vanhaalem <@t> prosensa.nl Wed Mar 7 01:54:37 2012 From: g.vanhaalem <@t> prosensa.nl (Geert van Haalem) Date: Wed Mar 7 01:54:47 2012 Subject: [Histonet] immunofluorescence mounting medium? Message-ID: <5F4E852313B6BC4B8C9916B69BAB4C1E05906F17@pro-ex02> Hi, I have used Prolong Gold and the Vectashield with good quality images. So I doubting whether it's the mounting media that causes the smeary effect. I could be the washing steps in your protocol or your choice of antibodies. I would also check your objective perhaps it has a correction ring to compensate for the refractive index. Regards, Geert the Netherlands > > Message: 6 > Date: Mon, 5 Mar 2012 09:33:38 -0800 > From: "Collette, Nicole M." > Subject: [Histonet] immunofluorescence mounting medium? > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > Hello, Esteemed Histonetters, > > I am trying to get nice publication-quality images of my immunofluorescent > tissue sections. I am currently using Prolong Gold, and after I let the > stuff cure for several days, my 100X oil-immersion images are still smeary, > even after taking into account the unevenness of the tissue section. I > don't seem to have this problem with colorimetric stains (histological > stains, LacZ, etc.), so I am thinking the mounting medium is part of the > problem? It seems to me that it never fully cures at the middle of the > coverslip? Does anyone have a recommendation for a mounting medium that > works better for this purpose? Any advice is appreciated. > > Thanks in advance, and Happy Monday! > > Sincerely, > Nicole Collette > Lawrence Livermore National Laboratory > collette2@llnl.gov > > > ------------------------------ > > Message: 7 > Date: Mon, 5 Mar 2012 17:40:26 +0000 > From: "Wen,Yujie" > Subject: RE: [Histonet] immunofluorescence mounting medium? > To: "Collette, Nicole M." , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > http://www.vectorlabs.com/catalog.aspx?catID=279 > > VECTASHIELD Mounting Media for Fluorescence > > This one works for me and was recommended by Leica technical support for > oil immersion image. > > -------- Original Message -------- > From: Collette, Nicole M. > Sent: Mon, Mar 5, 2012 12:35 PM > To: histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] immunofluorescence mounting medium? > > > Hello, Esteemed Histonetters, > > I am trying to get nice publication-quality images of my immunofluorescent > tissue sections. I am currently using Prolong Gold, and after I let the > stuff cure for several days, my 100X oil-immersion images are still smeary, > even after taking into account the unevenness of the tissue section. I > don't seem to have this problem with colorimetric stains (histological > stains, LacZ, etc.), so I am thinking the mounting medium is part of the > problem? It seems to me that it never fully cures at the middle of the > coverslip? Does anyone have a recommendation for a mounting medium that > works better for this purpose? Any advice is appreciated. > > Thanks in advance, and Happy Monday! > > Sincerely, > Nicole Collette > Lawrence Livermore National Laboratory > collette2@llnl.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 100, Issue 5 > **************************************** > -- Charity Wyatt Mohs Histotechnologist Jacksonville Skin Cancer Center, P.A./ Michael E. Lutz, M.D. (904)737-0111 ------------------------------ Message: 7 Date: Mon, 5 Mar 2012 15:39:28 -0500 From: "Virginia Chladek" Subject: [Histonet] Destain/Restain To: Message-ID: <9BB0A278800B0E41BBC28883FFAB45357DE363@exchsvr.wpu.local> Content-Type: text/plain; charset="us-ascii" **************************************** I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie ------------------------------ Message: 8 Date: Mon, 5 Mar 2012 12:52:18 -0800 (PST) From: Kim Tournear Subject: [Histonet] histology programs in Okalahoma To: Histonet Message-ID: <1330980738.90192.YahooMailNeo@web120205.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi everyone, Does anyone know of any histology training/programs in Oklahoma? Thanks in advance for all responses. ~Kim~? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ ------------------------------ Message: 9 Date: Mon, 5 Mar 2012 16:27:54 -0600 From: "Mitchell Jean A" Subject: [Histonet] 2012 Tri-State Symposium To: "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters: You are invited to join the histology societies of Wisconsin, Iowa and Minnesota as they host "An Ocean of Knowledge" at the 2012 Tri-State Symposium, May 2-4 at The Concourse Hotel in Madison, Wisconsin. For program, registration and vendor/exhibit information contact the following representatives: Wisconsin: Jean Mitchell (jmitchell@uwhealth.org) Iowa: Judi Stasko (judith.stasko@ars.usda.gov) Minnesota: Lois Rowe (rowe.lois@mayo.edu) Vendor/Exhibit: Dawn Schneider (dawn.schneider@ministryhealth.org) ------------------------------ Message: 10 Date: Mon, 5 Mar 2012 19:43:44 -0500 From: Komal Gada Subject: [Histonet] Question about slides To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Histo-netters, I'm looking for a source which sells slides for a Tissue Identification class. Does anyone have any leads on where to go for something like this? Thanks, Komal ------------------------------ Message: 11 Date: Mon, 5 Mar 2012 16:45:35 -0800 (PST) From: Tuyen Nguyen Subject: [Histonet] Greetings, friend! To: no-reply-37454207n00@flickr.com, lechi1962@yahoo.com, minhnguyet1818@yahoo.com, histonet@lists.utsouthwestern.edu, plucas@biopath.org, flastname@aerotek.com, svanlancker@memorialcare.org Message-ID: <1330994735.93267.yint-ygo-j2me@web162803.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii http://extendiendomanos.org/like.php?ficosyzycuge=38&qiqeli=625&jrexesofy=78 ------------------------------ Message: 12 Date: Mon, 5 Mar 2012 17:32:32 -0800 (PST) From: angela smith Subject: [Histonet] glacial acetic acid vs bouins To: histonet@lists.utsouthwestern.edu Message-ID: <1330997552.1563.YahooMailClassic@web125401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this.? Does anyone out there remember what dilution works best?? Thanks ------------------------------ Message: 13 Date: Tue, 6 Mar 2012 07:49:16 +0000 From: Jonathan Cremer Subject: RE: [Histonet] glacial acetic acid vs bouins To: angela smith , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Just an acid wash then, as in the wash after the methyl blue in an MSB or Mallory's trichrome? I have 1% and 0.5% for those stains, respectively. My guess would be that the concentration of acetic acid is not that important, just the fact that it lowers the pH of the water so specific stain does not wash out of the section. No idea what stain you're doing though. Might be something else entirely. Best --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens angela smith [we3smitty@yahoo.com] Verzonden: dinsdag 6 maart 2012 2:32 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] glacial acetic acid vs bouins I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 6 Mar 2012 06:35:33 -0600 From: "Giroux, Stacy" Subject: [Histonet] Smad4 (B-8) / DPC4 To: "histonet@lists.utsouthwestern.edu" Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3E0709AE9@AUSP03VMBX11.apptixhealth.net> Content-Type: text/plain; charset="iso-8859-1" Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital & Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ------------------------------ Message: 15 Date: Tue, 6 Mar 2012 08:31:52 -0500 From: "Gill, Caula A." Subject: RE: [Histonet] glacial acetic acid vs bouins To: "angela smith" , Message-ID: <087A9911BBAFDE4B8151CB148586E2C23AA011@MDGEN-EXCH1.marylandgeneral.org> Content-Type: text/plain; charset="iso-8859-1" What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well. Hope this helps . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, March 05, 2012 8:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glacial acetic acid vs bouins I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 06 Mar 2012 08:34:00 -0500 From: "Angela Bitting" Subject: Re: [Histonet] Smad4 (B-8) / DPC4 To: "histonet@lists.utsouthwestern.edu" , "Stacy Giroux" Message-ID: <4F55CBF8.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know. Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Giroux, Stacy" 3/6/2012 7:35 AM >>> Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital & Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 17 Date: Tue, 6 Mar 2012 08:34:25 -0500 From: "Gill, Caula A." Subject: RE: [Histonet] glacial acetic acid vs bouins To: "angela smith" , Message-ID: <087A9911BBAFDE4B8151CB148586E2C23AA014@MDGEN-EXCH1.marylandgeneral.org> Content-Type: text/plain; charset="iso-8859-1" What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well. Hope this helps . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, March 05, 2012 8:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glacial acetic acid vs bouins I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 6 Mar 2012 08:46:49 -0500 From: John Baker Subject: [Histonet] tissue processors To: Histonet@lists.utsouthwestern.edu Message-ID: <6274951A-A955-43AF-A745-9CD70ED76F23@umich.edu> Content-Type: text/plain; charset=us-ascii Hello Histoworld, What processors are people using to process bone or soft tissue samples for plastic? Do any of your processors allow for use of the monomer in the processor before embedding? What are your opinions, pro or cons, on the Tissue- Tek VIP5 or 6, the Leica ASP300, or the Thermo Pathcentre units? Hearing yours experiences in using them is greatly appreciated! My best, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ------------------------------ Message: 19 Date: Tue, 6 Mar 2012 14:01:49 +0000 From: "Chiriboga, Luis" Subject: [Histonet] IHC + control question To: "histonet@lists.utsouthwestern.edu" Message-ID: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Content-Type: text/plain; charset="us-ascii" Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis ------------------------------ Message: 20 Date: Tue, 6 Mar 2012 09:04:10 -0500 From: John Shelley Subject: [Histonet] CD11c and CD206 To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A605CE38EECB64B94485C02125A0C441AB810B061@LN-MAIL07.ln.burnham.org> Content-Type: text/plain; charset="iso-8859-1" Hi All, Was wondering if anyone has had experince using these antibodies. I have been given these antibodies from an investigator and they are from Ab Serotec. One is Rabbit and the other is Armenian Hamster it appears I can get the CD11c to work with a mild protease but the CD 206 does not appear to work under all the conditions I have tried. Too many to list but If someone has worked with both or one of these antibodies your help would be greatly appreciated. Kind regards! John Shelley ------------------------------ Message: 21 Date: Tue, 6 Mar 2012 07:42:54 -0700 From: "Breeden, Sara" Subject: [Histonet] Control Block Tracking To: Message-ID: <02C099024072804EA34F5906BAC30A413F1F5A@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" During a recent inspection of my department, the inspector "encouraged" me to develop a method of tracking which control tissue I used for a case (i.e., individually identifying the block and referencing that identifier on the slide). To be honest, I believe this to be overload, but since I'm probably going to have to abide by that "encouragement", I'd like to know if/how you identify which control block is used for a special stain. I can think of a simple method but wonder if any of you have used a really spectacular method that just blew the inspecting agency away. I'd promptly plagiarize it! Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 22 Date: Tue, 6 Mar 2012 09:57:22 -0500 From: Tom McNemar Subject: [Histonet] RE: Destain/Restain To: 'Virginia Chladek' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We do not destain h&e slides. We just take them back to water and stain right over top of the h&e. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Virginia Chladek Sent: Monday, March 05, 2012 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destain/Restain **************************************** I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 23 Date: Tue, 06 Mar 2012 10:04:12 -0500 From: "Angela Bitting" Subject: Re: [Histonet] IHC + control question To: "histonet@lists.utsouthwestern.edu" , "Luis Chiriboga" Message-ID: <4F55E11D.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset=US-ASCII My vote has always been for vimentin. See Dako's booklet IHC Staining Methods, 5th Ed, Chapter 19 addresses this. Angie >>> "Chiriboga, Luis" 3/6/2012 9:01 AM >>> Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 6 **************************************** From g.vanhaalem <@t> prosensa.nl Wed Mar 7 05:11:51 2012 From: g.vanhaalem <@t> prosensa.nl (Geert van Haalem) Date: Wed Mar 7 05:12:05 2012 Subject: [Histonet] replacing tween in the secondary hyb.solution In-Reply-To: <1330962363.93868.YahooMailClassic@web162105.mail.bf1.yahoo.com> References: <5F4E852313B6BC4B8C9916B69BAB4C1E05906E36@pro-ex02> <1330962363.93868.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: <5F4E852313B6BC4B8C9916B69BAB4C1E0590703D@pro-ex02> Tween indeed facilitates the dispersion. I was testing the Ventana autostainer and I got some inconsistent staining with patchiness of certain features instead of a homogeneous staining. I checked the presence and absence of tween to check if it somehow interferes with the liquid coverslip of Ventana. I just found out that also with a Z-stack that a significant depth variation is created somewhere during the run. It still just doesn't compete with the manual staining. Presumably the reason I could not find by eye a difference between with or without tween is that only 100 microliter is dispensed on the slide with on top the liquid coverslip and thus forcing the dispersion over the section. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Monday, March 05, 2012 4:46 PM To: histonet@lists.utsouthwestern.edu; Geert van Haalem Subject: Re: [Histonet] replacing tween in the secondary hyb.solution Not a good idea. The effect of Twenn is to facilitate the dispersion over the section of the reagent. If you do not use it the reagents may not reach all the surface and you may end with an uneven reaction or your results could be inconsistent. A small savings by not using Tween may mean that you could have to repeat the test and spend much more expensive reagents. Again, not a good idea. Ren? J. --- On Mon, 3/5/12, Geert van Haalem > wrote: From: Geert van Haalem > Subject: [Histonet] replacing tween in the secondary hyb.solution To: "histonet@lists.utsouthwestern.edu" > Date: Monday, March 5, 2012, 10:25 AM Hi, I currently did an experiment in which I left out Tween in de hybridisation solution of the secondary antibodies. I only made a solution of two Alexa in PBS. When imaging Bye eye I did not notice any decrease in signal compared to a hyb.solution with Tween. My intention is to replace tween with FBS, but can anybody tell me pro and cons for this idea. With regards, Geert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sameena.k.masood <@t> Vanderbilt.Edu Wed Mar 7 09:35:37 2012 From: sameena.k.masood <@t> Vanderbilt.Edu (Masood, Sameena Kathryn) Date: Wed Mar 7 09:34:57 2012 Subject: [Histonet] RE: xylene substitute for processing In-Reply-To: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> References: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> Message-ID: <1D0DCB1F02F24E4AB356F0F2EAEE1DC7060F39AA2B@its-hcwnem05.ds.Vanderbilt.edu> Kelly, Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. http://www.nationaldiagnostics.com/product_info.php?products_id=175 Good luck, Kathryn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly Sent: Monday, March 05, 2012 10:24 AM To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] xylene substitute for processing Greetings Histonet! Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? Thank you in advance for your help, Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Veterinary Pathobiology Texas Veterinary Medical Center Texas A&M University College Station, TX 77843-4467 979-862-3658 Office 979-845-5149 Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Wed Mar 7 09:39:07 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Wed Mar 7 09:39:17 2012 Subject: [Histonet] RE: IHC + control question In-Reply-To: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD1429C76E@MSGWCDCPMB22.nyumc.org> Message-ID: <3E6798F00C9F494399E96B720ECD1429CD58@MSGWCDCPMB22.nyumc.org> Consensus: Vimentin Thanks' to all who responded!! Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYUSOM/Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 Luis.Chiriboga@nyumc.org From: Chiriboga, Luis Sent: Tuesday, March 06, 2012 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: IHC + control question Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis From HornHV <@t> archildrens.org Wed Mar 7 09:46:08 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Mar 7 09:46:18 2012 Subject: [Histonet] muscle biopsy stains ATPase Message-ID: <25A4DE08332B19499904459F00AAACB719B2692A55@EVS1.archildrens.org> We are having a problem with our ATPase muscle stain. Does someone out there have a good procedure for this? And any tips or suggestions?? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From PAMarcum <@t> uams.edu Wed Mar 7 09:58:13 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Mar 7 09:58:20 2012 Subject: [Histonet] RE: xylene substitute for processing In-Reply-To: <1D0DCB1F02F24E4AB356F0F2EAEE1DC7060F39AA2B@its-hcwnem05.ds.Vanderbilt.edu> References: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> <1D0DCB1F02F24E4AB356F0F2EAEE1DC7060F39AA2B@its-hcwnem05.ds.Vanderbilt.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA323C82AC49@Mail2Node2.ad.uams.edu> Some people have issues with the smell and the other option is Anatech ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have very low odor and are also about the same price. We can't use the orange smell in our lab as some people have allergies and others just don't like it. Both work types work very well and it is a lab choice for the options, not a quality choice. Be aware most processor companies still require xylene in the clean cycle to keep your system healthy. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn Sent: Wednesday, March 07, 2012 9:36 AM To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: xylene substitute for processing Kelly, Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. http://www.nationaldiagnostics.com/product_info.php?products_id=175 Good luck, Kathryn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly Sent: Monday, March 05, 2012 10:24 AM To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] xylene substitute for processing Greetings Histonet! Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? Thank you in advance for your help, Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Veterinary Pathobiology Texas Veterinary Medical Center Texas A&M University College Station, TX 77843-4467 979-862-3658 Office 979-845-5149 Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From crossj <@t> KGH.KARI.NET Wed Mar 7 10:09:05 2012 From: crossj <@t> KGH.KARI.NET (Sparks, Joanne) Date: Wed Mar 7 10:09:18 2012 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe from histonet. From algranth <@t> email.arizona.edu Wed Mar 7 10:12:24 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Mar 7 10:12:36 2012 Subject: [Histonet] RE: xylene substitute for processing In-Reply-To: <1D0DCB1F02F24E4AB356F0F2EAEE1DC7060F39AA2B@its-hcwnem05.ds.Vanderbilt.edu> References: <869848DDBB7C5D4896A569A38B814E6905576330@CVMMB01.cvm.tamu.edu> <1D0DCB1F02F24E4AB356F0F2EAEE1DC7060F39AA2B@its-hcwnem05.ds.Vanderbilt.edu> Message-ID: <1E964E90-070C-470E-962F-8B997038AB5D@email.arizona.edu> I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite processing and it is great. I get it from Fisher. There has never been a problem with special stains but I use xylene on my stain lines. Andi Grantham On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote: > Kelly, > > Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. > > http://www.nationaldiagnostics.com/product_info.php?products_id=175 > > Good luck, > > Kathryn > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly > Sent: Monday, March 05, 2012 10:24 AM > To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] xylene substitute for processing > > Greetings Histonet! > > Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? > > Thank you in advance for your help, > Kelly > > Kelly S. Cross B.S., HT (ASCP) > Medical Laboratory Supervisor > Veterinary Pathobiology > Texas Veterinary Medical Center > Texas A&M University > College Station, TX 77843-4467 > 979-862-3658 Office > 979-845-5149 Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Wed Mar 7 10:14:43 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 7 10:15:47 2012 Subject: [Histonet] RE: xylene substitute for processing In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA323C82AC49@Mail2Node2.ad.uams.edu> Message-ID: One thing to take into consideration that many of the xylene substitutes take longer to clear than xylene. You may have to adjust your processing schedule. We use a xylene substitute with success. "Marcum, Pamela A" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/07/2012 07:58 AM To "'Masood, Sameena Kathryn'" , "Cross, Kelly" , "Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] RE: xylene substitute for processing Some people have issues with the smell and the other option is Anatech ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have very low odor and are also about the same price. We can't use the orange smell in our lab as some people have allergies and others just don't like it. Both work types work very well and it is a lab choice for the options, not a quality choice. Be aware most processor companies still require xylene in the clean cycle to keep your system healthy. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn Sent: Wednesday, March 07, 2012 9:36 AM To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: xylene substitute for processing Kelly, Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. http://www.nationaldiagnostics.com/product_info.php?products_id=175 Good luck, Kathryn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly Sent: Monday, March 05, 2012 10:24 AM To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] xylene substitute for processing Greetings Histonet! Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? Thank you in advance for your help, Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Veterinary Pathobiology Texas Veterinary Medical Center Texas A&M University College Station, TX 77843-4467 979-862-3658 Office 979-845-5149 Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LouroP <@t> Princeton.Huntingdon.com Wed Mar 7 10:55:26 2012 From: LouroP <@t> Princeton.Huntingdon.com (Louro, Pedro) Date: Wed Mar 7 10:56:47 2012 Subject: [Histonet] modified Davidson's fixative on testes Message-ID: Hello to all Histo techs and Happy early Histotechnology Professionals Day. I wanted to know if anyone out there is using modified Davidson's fixative to fix rat testes and staining with PAS. We have a pathologists that is looking for certain cells within the testes and thought that this might work. Thanks in advance for any information. Pedro Louro Technologist Histology/Immunohistochemistry ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** From melissa <@t> alliedsearchpartners.com Wed Mar 7 11:21:50 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed Mar 7 11:22:01 2012 Subject: [Histonet] Histotech Job Opening in TN-Permanent/Direct Hire Message-ID: Hello All, I have a permanent/full time job opening for a qualified Histotech in the Knoxville, TN area. Please contact me for details if you are interested. Everyone have a great day!!! -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From rford <@t> hcmhcares.org Wed Mar 7 11:23:48 2012 From: rford <@t> hcmhcares.org (Rhonda Ford) Date: Wed Mar 7 11:23:52 2012 Subject: [Histonet] SOP for release of tissues & consent form Message-ID: Does anyone have a procedure for the release of tissues along with a consent form that they like would like to share? Thanks in advance. -- Rhonda Ford, Histology Lab Henry County Hospital (765) 521-1148 From gu.lang <@t> gmx.at Wed Mar 7 11:24:57 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 7 11:25:03 2012 Subject: [Histonet] mesocolon lymphnode treatment with aceton Message-ID: <5B07917451A549249F5203DAEF32FE2B@dielangs.at> Dear all! Does anyone out there use this method for mesocolon clearing with aceton? I heard only a few facts about it and would like to hear about personal experience. The mesocolon is first fixed in NBF, then put in pure aceton, then treated with a teasing roller and put in a "pipe" with holes. The tissue is compressed to squeeze out the fat to form a kind of sausage. The sausage is sliced up, the slices are put into cassettes and processed. I don't know the exact details. If you have the access to this article, you can read more (I don't have). http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_ in_Rectal_Cancer__UICC.5.aspx or this: http://www.springerlink.com/content/748617k331l06143/ thank you Gudrun Lang Biomed. Analytikerin Histolab, Linz, Austria From kaitlin <@t> prometheushealthcare.com Wed Mar 7 12:04:06 2012 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Wed Mar 7 12:04:14 2012 Subject: [Histonet] New Histology Lab: NJ Message-ID: <00b701ccfc8c$b05bf7e0$1113e7a0$@prometheushealthcare.com> I am working with a histology practice that is opening a brand new lab in New Jersey. They are seeking experienced techs who reside in the area. Please feel free to e-mail for inquiries. Kaitlin (Kaitlin@prometheushealthcare.com) From bhartologist <@t> gmail.com Wed Mar 7 18:03:14 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Wed Mar 7 18:03:19 2012 Subject: [Histonet] Online HTL Message-ID: Tracy, Hello! I am currently doing IUPUI's HT program which is online as well, and I like it! :) I know they have an Associate of Science in Histotechnology program, in which case you have to be HT or HTL certified to apply. But how I understood it is if you are HT certified, which makes you eligible for this program, wouldn't you be qualified to sit for the HTL exam since you have to have at least an associates to take that? Not sure, but If you need information regarding this here is their link. And The program Director Debra Wood, who is my instructor as well is extremely nice and patient and can answer any questions you have. Good luck! http://www.iupui.edu/~bulletin/iupui/2010-2012/schools/medicine/undergraduate/histotechnology/index.shtml -Bharti Parihar From bhartologist <@t> gmail.com Wed Mar 7 18:13:32 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Wed Mar 7 18:13:36 2012 Subject: [Histonet] Need Employment Message-ID: Hello Histonetters! This site rocks and has been extremely helpful for me! Thanks to those that have sent me great information. I am encroaching towards the completion of my HT program and will be needing work soon. I'm planning on relocating from Cincinnati, OH to the San Francisco Bay area and am hoping someone can steer me in the right direction for work in that area. My program ends May 18th and I plan on sitting for my exam soon after and be in the bay area by tail end of may. Maybe this will have a good turn out of responses as well (fingers crossed). Thanks! -Bharti Parihar From garret.t.miyamoto <@t> us.army.mil Wed Mar 7 18:35:04 2012 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Wed Mar 7 18:35:14 2012 Subject: [Histonet] Re: Histonet Digest, Vol 100, Issue 9 In-Reply-To: <0M0J00H3N0V28O70@mail14.int.ps1.us.army.mil> References: <0M0J00H3N0V28O70@mail14.int.ps1.us.army.mil> Message-ID: <71d090facd51.4f577218@us.army.mil> Re: SOP for release of tissues and consent form Rhonda, We do not have an SOP but if you have a fax, I can send you a form that we use. Garret Miyamoto Tripler Army Medical Center ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Wednesday, March 7, 2012 8:04 am Subject: Histonet Digest, Vol 100, Issue 9 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: xylene substitute for processing (Marcum, Pamela A) > 2. unsubscribe (Sparks, Joanne) > 3. Re: RE: xylene substitute for processing > (Grantham, Andrea L - (algranth)) > 4. Re: RE: xylene substitute for processing (Jennifer MacDonald) > 5. modified Davidson's fixative on testes (Louro, Pedro) > 6. Histotech Job Opening in TN-Permanent/Direct Hire (Melissa > Phelan) 7. SOP for release of tissues & consent form (Rhonda Ford) > 8. mesocolon lymphnode treatment with aceton (Gudrun Lang) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Wed, 7 Mar 2012 15:58:13 +0000 > From: "Marcum, Pamela A" < > Subject: [Histonet] RE: xylene substitute for processing > To: "'Masood, Sameena Kathryn'" <, > "Cross, Kelly" <, "Histonet Listserv (E-mail) > (histonet@lists.utsouthwestern.edu)" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Some people have issues with the smell and the other option is Anatech ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have very low odor and are also about the same price. We can't use the orange smell in our lab as some people have allergies and others just don't like it. Both work types work very well and it is a lab choice for the options, not a quality choice. Be aware most processor companies still require xylene in the clean cycle to keep your system healthy. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn > Sent: Wednesday, March 07, 2012 9:36 AM > To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: xylene substitute for processing > > Kelly, > > Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. > > http://www.nationaldiagnostics.com/product_info.php?products_id=175 > > Good luck, > > Kathryn > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly > Sent: Monday, March 05, 2012 10:24 AM > To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] xylene substitute for processing > > Greetings Histonet! > > Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? > > Thank you in advance for your help, > Kelly > > Kelly S. Cross B.S., HT (ASCP) > Medical Laboratory Supervisor > Veterinary Pathobiology > Texas Veterinary Medical Center > Texas A&M University > College Station, TX 77843-4467 > 979-862-3658 Office > 979-845-5149 Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, > use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > > > > > ------------------------------ > > Message: 2 > Date: Wed, 7 Mar 2012 11:09:05 -0500 > From: "Sparks, Joanne" < > Subject: [Histonet] unsubscribe > To: < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Please unsubscribe from histonet. > > > > ------------------------------ > > Message: 3 > Date: Wed, 7 Mar 2012 08:12:24 -0800 > From: "Grantham, Andrea L - (algranth)" < > Subject: Re: [Histonet] RE: xylene substitute for processing > Cc: HISTONET < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite processing and it is great. I get it from Fisher. There has never been a problem with special stains but I use xylene on my stain lines. > > Andi Grantham > > > > > On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote: > > > Kelly, > > > > Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher. > > > > http://www.nationaldiagnostics.com/product_info.php?products_id=175 > > > > Good luck, > > > > Kathryn > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, Kelly > > Sent: Monday, March 05, 2012 10:24 AM > > To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) > > Subject: [Histonet] xylene substitute for processing > > > > Greetings Histonet! > > > > Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains? > > > > Thank you in advance for your help, > > Kelly > > > > Kelly S. Cross B.S., HT (ASCP) > > Medical Laboratory Supervisor > > Veterinary Pathobiology > > Texas Veterinary Medical Center > > Texas A&M University > > College Station, TX 77843-4467 > > 979-862-3658 Office > > 979-845-5149 Lab > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Wed, 7 Mar 2012 08:14:43 -0800 > From: Jennifer MacDonald < > Subject: Re: [Histonet] RE: xylene substitute for processing > To: "Marcum, Pamela A" < > Cc: "Histonet Listserv \(E-mail\) > \(histonet@lists.utsouthwestern.edu\)" > <, "'Masood, Sameena Kathryn'" > <, > histonet-bounces@lists.utsouthwestern.edu, "Cross, Kelly" > < > Message-ID: > < > Content-Type: text/plain; charset="US-ASCII" > > One thing to take into consideration that many of the xylene substitutes > take longer to clear than xylene. You may have to adjust your processing > schedule. We use a xylene substitute with success. > > > > > "Marcum, Pamela A" < > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/07/2012 07:58 AM > > To > "'Masood, Sameena Kathryn'" <, "Cross, > Kelly" <, "Histonet Listserv (E-mail) > (histonet@lists.utsouthwestern.edu)" < > cc > > Subject > [Histonet] RE: xylene substitute for processing > > > > > > > Some people have issues with the smell and the other option is Anatech > ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have > very low odor and are also about the same price. We can't use the orange > smell in our lab as some people have allergies and others just don't like > it. Both work types work very well and it is a lab choice for the > options, not a quality choice. Be aware most processor companies still > require xylene in the clean cycle to keep your system healthy. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masood, > Sameena Kathryn > Sent: Wednesday, March 07, 2012 9:36 AM > To: Cross, Kelly; Histonet Listserv (E-mail) > (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: xylene substitute for processing > > Kelly, > > Histo-Clear can be directly substituted for Xylene at every point in > processing and staining. It's completely non-toxic (even has a food grade > rating), is made from oranges, and smells delicious. You don't have to > work in a hood and it seems to make tissue less brittle than Xylene also. > Some guys in our Histology Core told us about it and we have been happy > with it. You can purchase it from the manufacturer, National Diagnostics, > or Fisher. > > http://www.nationaldiagnostics.com/product_info.php?products_id=175 > > Good luck, > > Kathryn > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cross, > Kelly > Sent: Monday, March 05, 2012 10:24 AM > To: Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] xylene substitute for processing > > Greetings Histonet! > > Does anyone use xylene substitutes for routine over-night processing? If > so, what do you use and does it have any adverse effect your special > stains? > > Thank you in advance for your help, > Kelly > > Kelly S. Cross B.S., HT (ASCP) > Medical Laboratory Supervisor > Veterinary Pathobiology > Texas Veterinary Medical Center > Texas A&M University > College Station, TX 77843-4467 > 979-862-3658 Office > 979-845-5149 Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, > use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 7 Mar 2012 11:55:26 -0500 > From: "Louro, Pedro" < > Subject: [Histonet] modified Davidson's fixative on testes > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Hello to all Histo techs and Happy early Histotechnology Professionals > Day. > > > > I wanted to know if anyone out there is using modified Davidson's > fixative to fix rat testes and staining with PAS. > > We have a pathologists that is looking for certain cells within the > testes and thought that this might work. > > > > Thanks in advance for any information. > > > > Pedro Louro > > Technologist > > Histology/Immunohistochemistry > > > > > > ******************************************************************************************** > Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect > ******************************************************************************************** > > LEGAL NOTICE > This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. > The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. > Registered in England No. 1815730 > VAT Reg. No. GB425507072 > Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS > ********************************************************************** > > > > ------------------------------ > > Message: 6 > Date: Wed, 07 Mar 2012 12:21:50 -0500 > From: Melissa Phelan < > Subject: [Histonet] Histotech Job Opening in TN-Permanent/Direct Hire > To: < > Message-ID: < > Content-Type: text/plain; charset="US-ASCII" > > Hello All, > > I have a permanent/full time job opening for a qualified Histotech in the > Knoxville, TN area. Please contact me for details if you are interested. > Everyone have a great day!!! > -- > Melissa Phelan, President Laboratory Staffing > Allied Search Partners > http://www.linkedin.com/in/melissaphelan > P: 888-388-7571 > F: 888-388-7572 > C: 407-697-1175 > www.alliedsearchpartners.com > > > > > > > ------------------------------ > > Message: 7 > Date: Wed, 7 Mar 2012 12:23:48 -0500 > From: Rhonda Ford < > Subject: [Histonet] SOP for release of tissues & consent form > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > Content-Type: text/plain; charset=ISO-8859-1 > > Does anyone have a procedure for the release of tissues along with a > consent form that they like would like to share? Thanks in advance. > > -- > Rhonda Ford, Histology Lab > Henry County Hospital > (765) 521-1148 > > > ------------------------------ > > Message: 8 > Date: Wed, 7 Mar 2012 18:24:57 +0100 > From: "Gudrun Lang" < > Subject: [Histonet] mesocolon lymphnode treatment with aceton > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Dear all! > > Does anyone out there use this method for mesocolon clearing with aceton? I > heard only a few facts about it and would like to hear about personal > experience. > > The mesocolon is first fixed in NBF, then put in pure aceton, then treated > with a teasing roller and put in a "pipe" with holes. The tissue is > compressed to squeeze out the fat to form a kind of sausage. > > The sausage is sliced up, the slices are put into cassettes and processed. > > I don't know the exact details. If you have the access to this article, you > can read more (I don't have). > > http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_ > in_Rectal_Cancer__UICC.5.aspx > > or this: > > http://www.springerlink.com/content/748617k331l06143/ > > > > thank you > > > > Gudrun Lang > > > > Biomed. Analytikerin > > Histolab, Linz, Austria > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 100, Issue 9 > **************************************** From tuyenmai77 <@t> yahoo.com Wed Mar 7 22:29:41 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Wed Mar 7 22:29:44 2012 Subject: [Histonet] Hello, friend! Message-ID: <1331180981.82418.yint-ygo-j2me@web162803.mail.bf1.yahoo.com> http://nutree-herb.com/like.php?rjmegyfegigib=71&mozigaw=374&ljqykeh=62 From NHeath <@t> Lifespan.org Thu Mar 8 05:04:07 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Mar 8 05:04:17 2012 Subject: [Histonet] muscle biopsy stains ATPase In-Reply-To: <25A4DE08332B19499904459F00AAACB719B2692A55@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719B2692A55@EVS1.archildrens.org> Message-ID: <130E8991F210424096EFC6F42EA33B2408B7C3D5@LSCOEXCH1.lsmaster.lifespan.org> hope this helps :) Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, March 07, 2012 10:46 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] muscle biopsy stains ATPase We are having a problem with our ATPase muscle stain. Does someone out there have a good procedure for this? And any tips or suggestions?? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Thu Mar 8 06:57:05 2012 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Mar 8 06:57:15 2012 Subject: [Histonet] ATPase reaction Message-ID: <8CECB4218CC194F-1F28-772F@webmail-d061.sysops.aol.com> Hazel Horn asks about the ATPase reaction: Years ago we had problem with our ATPase reaction on F/S of muscle biopsies. We discovered that in keeping the substrate at minus 20oC in a freezer, and getting it out and letting it warm up to room temperature before weighing a small amount out for the procedure, and then putting it back in the freezer, in doing that all the time, we had inadvertantly inactivated the ATPase substrate. Its best to aliquot it out when you first receive it from Sigma, or whoever, and keep those vials frozen, getting one out to use every time you do the reaction. Other than that, I would think the solutions are suspect, too old or wrong pH or something like that. Regards Michael Titford Pathology - USA Mobile AL From mmatthannah <@t> yahoo.com Thu Mar 8 07:37:43 2012 From: mmatthannah <@t> yahoo.com (Michelle Becker) Date: Thu Mar 8 07:37:47 2012 Subject: [Histonet] articles needed please Message-ID: <1331213863.29399.YahooMailClassic@web82306.mail.mud.yahoo.com> My daughter is doing a paper on "repetitive motion injuries" I thought what better than the AO Microtomes, if anyone has any info or can suggest any web sites with articles on ergonomic issues histo techs have experienced over the years we would greatly appreciate it,? Thanks Michelle From joelleweaver <@t> hotmail.com Thu Mar 8 07:44:26 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 8 07:44:30 2012 Subject: [Histonet] articles needed please In-Reply-To: <1331213863.29399.YahooMailClassic@web82306.mail.mud.yahoo.com> References: <1331213863.29399.YahooMailClassic@web82306.mail.mud.yahoo.com> Message-ID: I do not know what level of depth this paper needs to be, but these are good background articles. http://ergo.human.cornell.edu/AHProjects/Hospital_Ergonomics/lab_radiology.pdf http://www.uos.harvard.edu/ehs/ih/labergo_microtome.shtmlhttp://www.d.umn.edu/ehso/ergonomics/microtomy.html http://www.umdnj.edu/eohssweb/publications/LabErgonomics.pdf https://fpm-www3.fpm.wisc.edu/safety/occupationalHealth/Ergonomics/LaboratoryErgonomics/LaboratoryErgonomicStressors/tabid/85/Default.aspx> Date: Thu, 8 Mar 2012 05:37:43 -0800 > From: mmatthannah@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] articles needed please > > My daughter is doing a paper on "repetitive motion injuries" I thought what better than the AO Microtomes, if anyone has any info or can suggest any web sites with articles on ergonomic issues histo techs have experienced over the years we would greatly appreciate it, Thanks Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Mar 8 07:52:06 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 8 07:52:16 2012 Subject: [Histonet] Online HTL In-Reply-To: References: Message-ID: The ASCP HTL cert. eligibility criteria listed by BOC is a bachelors. http://www.ascp.org/Board-of-Certification/GetCertified#tabs-1Not sure if there is an online available, check the programs listed on NSH, nsh.org , but if you go into a program with a bachelor's with specific biological science, math, chemistry and they approve your credits, I think that route is still available with 1 year experience. > Date: Wed, 7 Mar 2012 19:03:14 -0500 > From: bhartologist@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Online HTL > > Tracy, > Hello! I am currently doing IUPUI's HT program which is online > as well, and I like it! :) I know they have an Associate of Science in > Histotechnology program, in which case you have to be HT or HTL certified > to apply. But how I understood it is if you are HT certified, which makes > you eligible for this program, wouldn't you be qualified to sit for the HTL > exam since you have to have at least an associates to take that? Not sure, > but If you need information regarding this here is their link. And The > program Director Debra Wood, who is my instructor as well is extremely nice > and patient and can answer any questions you have. Good luck! > > http://www.iupui.edu/~bulletin/iupui/2010-2012/schools/medicine/undergraduate/histotechnology/index.shtml > > > -Bharti Parihar > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Thu Mar 8 07:55:06 2012 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Mar 8 07:55:18 2012 Subject: [Histonet] mesocolon lymphnode treatment with aceton In-Reply-To: <5B07917451A549249F5203DAEF32FE2B@dielangs.at> References: <5B07917451A549249F5203DAEF32FE2B@dielangs.at> Message-ID: <4F5865DA020000AF00007F99@nodcdmg2.no.trinity-health.org> I would also be interested if anyone is using this method and their results. I have printed off the article and shared the information with our supervising pathologist. I have found dermal needle rollers on line but wonder if the maximum 2 mm length will do the job of perforating the fat. Also would like more information on the compression device used. Thanks......Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Gudrun Lang" 3/7/2012 11:24 AM >>> Dear all! Does anyone out there use this method for mesocolon clearing with aceton? I heard only a few facts about it and would like to hear about personal experience. The mesocolon is first fixed in NBF, then put in pure aceton, then treated with a teasing roller and put in a "pipe" with holes. The tissue is compressed to squeeze out the fat to form a kind of sausage. The sausage is sliced up, the slices are put into cassettes and processed. I don't know the exact details. If you have the access to this article, you can read more (I don't have). http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_ in_Rectal_Cancer__UICC.5.aspx or this: http://www.springerlink.com/content/748617k331l06143/ thank you Gudrun Lang Biomed. Analytikerin Histolab, Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Mar 8 10:00:58 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Mar 8 10:01:19 2012 Subject: [Histonet] Goldner's Trichrome for Osteoid Message-ID: <474803E6-7C4E-4C41-B476-E198E8CD02E3@email.arizona.edu> Hopefully a hard tissue guru is out there and can help with this. A PI here is asking about doing a Goldner's stain on mouse femur. I'm not set up to cut sections of undecalcified bone so my questions are: 1. can this stain be done successfully on decalcified bone? 2. if so, what type of decal should be used - EDTA? 3. I have googled the stain but didn't see what the preferred thickness was for this stain. Need this info fairly soon! The bones are on the way. Thanks!!! Andi G. From relia1 <@t> earthlink.net Thu Mar 8 10:23:52 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Mar 8 10:24:01 2012 Subject: [Histonet] Territory Sales Rep needed in Southern NJ. Can you help? Message-ID: Hi Histonetters! I hope everyone is having a great day and enjoying some free lunches celebrating histotechnology professionals day! I have a new position I would like to tell you about. Territory Sales Rep Histology-Southern NJ RELIA is working with a growing lab located in Southern NJ. They have asked for assistance with their Territory Sales Rep position. This sales representative must possess a strong background in cancer diagnostic testing/services and be comfortable speaking to doctors across multiple specialties, including gastroenterology, urology, and dermatology. Be ready to hit the ground running! Primary responsibilities of the position: Foster the growth of our companies services by closing new accounts, maintaining, and increasing sales volume of current accounts Have the ability to discuss the various testing in anatomic pathology thoroughly from a technical and laymen's perspective to potential clients Meet and exceed all sales quotas provided by upper sales management for all of our specialties Participate in conferences and local meetings to generate sales leads for potential clients and increase awareness of company services Maintain all prospects and current client information in sales database on a daily basis, attend sales meetings, and respond to emails from sales management and staff in a timely manner Position Requirements: Minimum education: Bachelor's degree, preferably in field of science Minimum 4+ years medical/laboratory sales experience with proven track record-preferably in anatomic pathology Knowledge of laboratory/medical industry, including relevant diagnostic tests, pathology services, disease states, and cancer treatments Excellent communication skills at all business levels Superior presentation and influential skills a must Well-organized, ability to self-manage and maintain a professional, tactful stance at all times, polished appearance Willing to travel Contacts for potential clients in specialties that Our services, including gastroenterology, urology, and dermatology, a definite plus! We offer all sales representatives a competitive salary and commission structure with a comprehensive benefits package. For more information please contact Pam Barker toll free at 866-607-3542 or relia1@earthlink.net Thanks! Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.twitter.com/pamatrelia From DSiena <@t> statlab.com Thu Mar 8 10:33:00 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Mar 8 10:33:08 2012 Subject: [Histonet] mesocolon lymphnode treatment with aceton In-Reply-To: <4F5865DA020000AF00007F99@nodcdmg2.no.trinity-health.org> References: <5B07917451A549249F5203DAEF32FE2B@dielangs.at> <4F5865DA020000AF00007F99@nodcdmg2.no.trinity-health.org> Message-ID: If anyone has access to the article, I would like to get a copy if you don't mind. thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, March 08, 2012 7:55 AM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mesocolon lymphnode treatment with aceton I would also be interested if anyone is using this method and their results. I have printed off the article and shared the information with our supervising pathologist. I have found dermal needle rollers on line but wonder if the maximum 2 mm length will do the job of perforating the fat. Also would like more information on the compression device used. Thanks......Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Gudrun Lang" 3/7/2012 11:24 AM >>> Dear all! Does anyone out there use this method for mesocolon clearing with aceton? I heard only a few facts about it and would like to hear about personal experience. The mesocolon is first fixed in NBF, then put in pure aceton, then treated with a teasing roller and put in a "pipe" with holes. The tissue is compressed to squeeze out the fat to form a kind of sausage. The sausage is sliced up, the slices are put into cassettes and processed. I don't know the exact details. If you have the access to this article, you can read more (I don't have). http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_ in_Rectal_Cancer__UICC.5.aspx or this: http://www.springerlink.com/content/748617k331l06143/ thank you Gudrun Lang Biomed. Analytikerin Histolab, Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhartologist <@t> gmail.com Thu Mar 8 11:03:18 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Thu Mar 8 11:03:25 2012 Subject: [Histonet] Immunocal and Her 2 Neu FISH Message-ID: Does anyone know if bone decalcified in Immunocal is ok to do Her 2 Neu FISH? Thanks Histonetters! :) -Bharti Parihar From louise.renton <@t> gmail.com Thu Mar 8 11:40:21 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Thu Mar 8 11:40:26 2012 Subject: [Histonet] Goldner's Trichrome for Osteoid In-Reply-To: <474803E6-7C4E-4C41-B476-E198E8CD02E3@email.arizona.edu> References: <474803E6-7C4E-4C41-B476-E198E8CD02E3@email.arizona.edu> Message-ID: As far as i know, osteoid will not show up on a decalcified section. On Thu, Mar 8, 2012 at 6:00 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Hopefully a hard tissue guru is out there and can help with this. > > A PI here is asking about doing a Goldner's stain on mouse femur. I'm not > set up to cut sections of undecalcified bone so my questions are: > 1. can this stain be done successfully on decalcified bone? > 2. if so, what type of decal should be used - EDTA? > 3. I have googled the stain but didn't see what the preferred thickness > was for this stain. > > Need this info fairly soon! The bones are on the way. > > Thanks!!! > > Andi G. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From cornettl <@t> hotmail.com Thu Mar 8 12:58:14 2012 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Thu Mar 8 12:58:20 2012 Subject: [Histonet] fahima Message-ID: Business Opportunities - Start Your Own Business http://jadehurtz.com/pisces.php?nufugmailID=701 ____________ Whod you give the baggage to?Nobody. kodiak adelia Thu, 8 Mar 2012 19:58:14 From dmlaud <@t> gmail.com Thu Mar 8 13:16:50 2012 From: dmlaud <@t> gmail.com (Damien) Date: Thu Mar 8 13:16:55 2012 Subject: [Histonet] Re:Goldner's Trichrome for Osteoid Message-ID: Hi Andi, Yes, you can stain and differentiate osteoid using decalcified bone sections. You can see osteoid after staining with any trichrome technique, H&E or Toluidine blue. The structure does not disappear with decalcification. However, do not expect the Goldner?s stain to mimic the staining pattern, or have the sharp contrast, that what would be observed when using a calcified bone section. For decalcified sections, I personally like the Gomori one step with Aniline Blue (osteoid will stain blue). 1.Yes (with some exceptions) 2. Formic acid works well 3. Try 5 microns -Damien L. -- Damien Laudier Laudier Histology www.LaudierHistology.com From patjnm <@t> gwumc.edu Thu Mar 8 13:20:27 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Thu Mar 8 13:20:34 2012 Subject: [Histonet] PAS Rat Testes Message-ID: <4F58C02C.DB55.001F.1@gwumc.edu> If the path does not want to use Davidsons and still wants the acetic acid, perhaps Carnoys, Bouins, Methacarn? Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From NHeath <@t> Lifespan.org Thu Mar 8 13:33:29 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Mar 8 13:33:37 2012 Subject: [Histonet] ATPase reaction In-Reply-To: <8CECB4218CC194F-1F28-772F@webmail-d061.sysops.aol.com> References: <8CECB4218CC194F-1F28-772F@webmail-d061.sysops.aol.com> Message-ID: <130E8991F210424096EFC6F42EA33B2408B7C534@LSCOEXCH1.lsmaster.lifespan.org> @ Hazel Horn...sorry about not attaching the procedure :/ Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Thursday, March 08, 2012 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ATPase reaction Hazel Horn asks about the ATPase reaction: Years ago we had problem with our ATPase reaction on F/S of muscle biopsies. We discovered that in keeping the substrate at minus 20oC in a freezer, and getting it out and letting it warm up to room temperature before weighing a small amount out for the procedure, and then putting it back in the freezer, in doing that all the time, we had inadvertantly inactivated the ATPase substrate. Its best to aliquot it out when you first receive it from Sigma, or whoever, and keep those vials frozen, getting one out to use every time you do the reaction. Other than that, I would think the solutions are suspect, too old or wrong pH or something like that. Regards Michael Titford Pathology - USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Thu Mar 8 13:37:09 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Mar 8 13:37:13 2012 Subject: [Histonet] ATPase reaction In-Reply-To: <130E8991F210424096EFC6F42EA33B2408B7C534@LSCOEXCH1.lsmaster.lifespan.org> References: <8CECB4218CC194F-1F28-772F@webmail-d061.sysops.aol.com> <130E8991F210424096EFC6F42EA33B2408B7C534@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <130E8991F210424096EFC6F42EA33B2408B7C536@LSCOEXCH1.lsmaster.lifespan.org> well...looks like I don't know how to attach things so they will post on histonet...Hazel Horn I sent my procedure to your email too :) Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, March 08, 2012 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ATPase reaction @ Hazel Horn...sorry about not attaching the procedure :/ Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Thursday, March 08, 2012 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ATPase reaction Hazel Horn asks about the ATPase reaction: Years ago we had problem with our ATPase reaction on F/S of muscle biopsies. We discovered that in keeping the substrate at minus 20oC in a freezer, and getting it out and letting it warm up to room temperature before weighing a small amount out for the procedure, and then putting it back in the freezer, in doing that all the time, we had inadvertantly inactivated the ATPase substrate. Its best to aliquot it out when you first receive it from Sigma, or whoever, and keep those vials frozen, getting one out to use every time you do the reaction. Other than that, I would think the solutions are suspect, too old or wrong pH or something like that. Regards Michael Titford Pathology - USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.DeMint <@t> phci.org Thu Mar 8 13:49:10 2012 From: Valerie.DeMint <@t> phci.org (DeMint, Valerie S) Date: Thu Mar 8 13:49:18 2012 Subject: [Histonet] ThermScientific HistoScreens-printing quality problems Message-ID: <657B95ED46A98B4D9F44EFC67EDCAADD04AFC797@RWDEX3.WHS.phci.org> Hi I am looking for help with a cassette printing problem that we are experiencing. We use the Thermo Histo Screen cassettes and the Surgipath VCP 2001 cassette printer. Case numbers are quite smudged or missing completely when the batch has finished processing. This is only happening to the HistoScreens because we also use a routine cassette from another manufacturer and there are no problems with print quality. I have switched lot numbers of the printer ink ribbon and continue to have problems. It is not happening with every cassette on the batch so I do not believe it to be the printer itself. Is any one else experiencing printing quality issues with these cassettes? your help is appreciated, Valerie DeMint, HT(ASCP) Histology Lead Waukesha Memorial Hospital Waukesha, WI This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From ratliffjack <@t> hotmail.com Thu Mar 8 13:55:11 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 8 13:55:15 2012 Subject: [Histonet] Goldner's Trichrome for Osteoid In-Reply-To: <474803E6-7C4E-4C41-B476-E198E8CD02E3@email.arizona.edu> References: <474803E6-7C4E-4C41-B476-E198E8CD02E3@email.arizona.edu> Message-ID: Andi, Your PI has requested the Goldner's trichrome stain because it provides a stark contrast between the green (light-green SF yellowish) stained mineralized component of bone and the red (acid fuchsin-ponceau) stained newly formed unmineralized "bone-like" dense collagen fibers (osteoid) that will eventually mature and mineralize to form bone. Now if you subject mineralized bone to an acid solution, the process of demineralization begins and acts to remove the mineral (calcium and phosphorus) content. The acid will also have an effect on the soft tissue components, but the effect varies depending upon the acid concentration and certain properties of tissues. Nevertheless, the most notable effect of acid decalcification will be seen as a change in the density of bone and demonstrated by stain uptake and penetration. If you have to demineralize your bone in order to cut sections because you do not have the capabilities to cut undemineralized bone, then you need a stain that provides the best contrast for this now demineralized state of bone. Once the bone has been decalcified, you essentially have now demineralized bone (less dense) and still unmineralized "bone-like" collagen fibers, all of which will now be contrasted differently with the Goldner's stain. While an H&E can be used to distinguish bone that was once mineralized (old bone) from unmineralized "bone-like" collagen fibers (osteoid or newly forming bone), there is not enough of a stark contrast and especially if you are wanting to use some form of image analysis software. With that said, the best stain then to use in this situation is a Masson's trichrome where the bone stains blue (aniline blue) and the osteoid stains red (biebrich-scarlet). With regards to the demineralization method, the EDTA will take too long to arrive at the same result that a simple 5% formic acid will provide. Also, your sections will be cut at a standard 5 microns. Hope this information helps you to get what you need and understand a little more of why you need what you need! :) Best Regards, Jack From: algranth@email.arizona.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 8 Mar 2012 08:00:58 -0800 Subject: [Histonet] Goldner's Trichrome for Osteoid Hopefully a hard tissue guru is out there and can help with this. A PI here is asking about doing a Goldner's stain on mouse femur. I'm not set up to cut sections of undecalcified bone so my questions are: 1. can this stain be done successfully on decalcified bone? 2. if so, what type of decal should be used - EDTA? 3. I have googled the stain but didn't see what the preferred thickness was for this stain. Need this info fairly soon! The bones are on the way. Thanks!!! Andi G. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Thu Mar 8 14:06:54 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Mar 8 14:06:59 2012 Subject: [Histonet] RE: ThermScientific HistoScreens-printing quality problems In-Reply-To: <657B95ED46A98B4D9F44EFC67EDCAADD04AFC797@RWDEX3.WHS.phci.org> References: <657B95ED46A98B4D9F44EFC67EDCAADD04AFC797@RWDEX3.WHS.phci.org> Message-ID: This is an issue with the cassettes not your printer or ribbons. If you take a straight edge and put it up against the edge of the cassette you will see that he cassette is concaving causing the styles to loose contact with the cassette. It is more of a problem at the middle top edge of the cassette. It is only with the histoscreen cassettes and I bet Thermo is telling you that this is the first they are hearing about this problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeMint, Valerie S Sent: Thursday, March 08, 2012 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThermScientific HistoScreens-printing quality problems Hi I am looking for help with a cassette printing problem that we are experiencing. We use the Thermo Histo Screen cassettes and the Surgipath VCP 2001 cassette printer. Case numbers are quite smudged or missing completely when the batch has finished processing. This is only happening to the HistoScreens because we also use a routine cassette from another manufacturer and there are no problems with print quality. I have switched lot numbers of the printer ink ribbon and continue to have problems. It is not happening with every cassette on the batch so I do not believe it to be the printer itself. Is any one else experiencing printing quality issues with these cassettes? your help is appreciated, Valerie DeMint, HT(ASCP) Histology Lead Waukesha Memorial Hospital Waukesha, WI This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From modz9636 <@t> gmail.com Thu Mar 8 15:51:46 2012 From: modz9636 <@t> gmail.com (M.O.) Date: Thu Mar 8 15:51:49 2012 Subject: [Histonet] Optimizing H&E Stains for undecalcified bone Message-ID: Hello Histonetters! I am new to staining and can't seem to get good H&E stains from deplacticized undecalcified bone with an implant. It seems as though the hematoxylin is staining too dark. Any feedback would be much appreciated! The protocol I am using is for after the deplasticization step and says: 100% EtOH for 3 mins, 70% EtOH for 3 mins Hematoxylin for 6mins, Rinse, .25% Acid Ethanol dip 8-10 times, Rinse 95% EtOH 1min Eosin for 2mins Quick Dip 95%, 95%, 100% 100% EtOH Soak in ProPar for 15+mins Sincerely, Merissa From ratliffjack <@t> hotmail.com Thu Mar 8 16:31:16 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 8 16:31:27 2012 Subject: [Histonet] Optimizing H&E Stains for undecalcified bone In-Reply-To: References: Message-ID: Merissa, You should very easily be able to get a nice looking H&E from deplasticized undemineralized bone sections. In fact, try the protocol that I routinely use for my MMA embedded specimens: Deplastification x 3 changes each for 15-30 minutes @ 55-60C w/ gentle dip and dunk at half way mark for each change 100% EtOH @ RT for 5 min 95% EtOH @ RT for 5 min 70% EtOH @ RT for 5 min dH2O @ RT for 5 min Hematoxylin 2 (from Richard Allen @ Fisher Sci) @ RT for 3 min Running Tap Water Rinse for 1 min Clarifier 2 (from Richard Allen @ Fisher Sci) @ RT for 20-30 seconds with a gentle dip and dunk at half way mark Running Tap Water Rinse for 1 min Bluing Solution (from Richard Allen @ Fisher Sci) @ RT for 20-30 seconds with a gentle dip and dunk at half way mark Running Tap Water Rinse for 1 min Eosin Y (from Richard Allen @ Fisher Sci) @ RT for 40 seconds 70% EtOH @ RT with 10 gentle dip and dunks 95% EtOH @ RT with 10 gentle dip and dunks 100% EtOH @ RT with 10 gentle dip and dunks 100% EtOH @ RT for 30 seconds Xylenes @ RT for 3 min Xylenes @ RT for 3 min Coverslip Your problem could be the type of hematoxylin you are using (progressive vs. regressive) and the length of time in solution. I would also recommend you try a Von Kossa with a MacNeal's tetrachrome counterstain, Goldner's trichrome, and maybe a toluidine blue stain with these type of sections. Best Regards, Jack Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 8, 2012, at 3:51 PM, "M.O." wrote: > Hello Histonetters! I am new to staining and can't seem to get good H&E > stains from deplacticized undecalcified bone with an implant. It seems as > though the hematoxylin is staining too dark. Any feedback would be much > appreciated! > > The protocol I am using is for after the deplasticization step and says: > > 100% EtOH for 3 mins, 70% EtOH for 3 mins > > Hematoxylin for 6mins, Rinse, .25% Acid Ethanol dip 8-10 times, Rinse > > 95% EtOH 1min > > Eosin for 2mins > > Quick Dip 95%, 95%, 100% 100% EtOH > > Soak in ProPar for 15+mins > > > Sincerely, > Merissa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tuyenmai77 <@t> yahoo.com Fri Mar 9 06:02:52 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Fri Mar 9 06:02:56 2012 Subject: [Histonet] Hi, friend! Message-ID: <1331294572.33300.yint-ygo-j2me@web162806.mail.bf1.yahoo.com> Hi, friend! http://joyeriamegg.com.ar/party.php?evowutacu=27&ykodiz=574&rebarahaw=42 From NSEARCY <@t> swmail.sw.org Fri Mar 9 06:19:56 2012 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Mar 9 06:20:13 2012 Subject: [Histonet] Question Message-ID: <4F59A10C.5D38.00EF.0@swmail.sw.org> IS there a CPT code for performing radiological exams on processed breast cassettes for calcium? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From tajibade <@t> echd.org Fri Mar 9 09:14:41 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Fri Mar 9 09:14:50 2012 Subject: [Histonet] Question In-Reply-To: <4F59A10C.5D38.00EF.0@swmail.sw.org> References: <4F59A10C.5D38.00EF.0@swmail.sw.org> Message-ID: I think your radiology dept suppose to do the radiological exam on the core breast biopsies after the specimen is taken from the patient, radiology bills for this service, they only send the x ray film to the histology lab for pathologists to identify the calcified area of the breast. Tunde Ajibade BS, HTL(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, March 09, 2012 6:20 AM To: histonet@lists.utsouthwestern.edu Cc: Patricia Webster Subject: [Histonet] Question IS there a CPT code for performing radiological exams on processed breast cassettes for calcium? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From relia1 <@t> earthlink.net Fri Mar 9 09:35:56 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Mar 9 09:36:52 2012 Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition. Happy Histotechnology Professionals Day Saturday March 10. Message-ID: <9CEF464EE6B34AB0801E302FA22FD042@ownerf1abaad51> Hi Histonetters!! How are you? I wanted to take a moment and say Happy Histotechnology Professionals Day. I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! I am curious as to how you are planning on celebrating. I started last week by contacting each and every one of my 1000+ client/employers to let them know that March 10 is The 3rd Annual National Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are now!! . Since HPD falls on a Saturday maybe it is a good time to reflect on your career, where you are, what you want, where you would like to be and how are you going to get there? If you aren?t considering a job change: * Is it time to join/renew your NSH membership? * Check out your state society? * Post something to the Histonet? * Take some CEUs to stay up to date and learn some new skills? * Get that state license for the place you want to move to one day? * Start studying for that QIHC? If you are or might be considering a job change now or in the near future: * Does your resume need to be updated? * Have you considered what you are looking for and where? * Have you gotten the state license if it is required before you go? * Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: relia1@earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. As always I have amazing opportunities with outstanding clients so be on the lookout for a regular bulletin listing all of my current openings and give me a call or shoot me an email if you want a preview! Don?t forget to shoot me back an email to share what you did for Histotechnology Professionals Day. I really want to know J Happy Histo Day!! >Pam From dmlaud <@t> laudierhistology.com Fri Mar 9 09:56:44 2012 From: dmlaud <@t> laudierhistology.com (Damien) Date: Fri Mar 9 09:56:49 2012 Subject: [Histonet] Question In-Reply-To: References: <4F59A10C.5D38.00EF.0@swmail.sw.org> Message-ID: On Fri, Mar 9, 2012 at 10:14 AM, Tunde Ajibade wrote: > I think your radiology dept suppose to do the radiological exam on the > core breast biopsies after the specimen is taken from the patient, > radiology bills for this service, they only send the x ray film to the > histology lab for pathologists to identify the calcified area of the breast. > > > Tunde Ajibade BS, HTL(ASCP)QIHC > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy > Sent: Friday, March 09, 2012 6:20 AM > To: histonet@lists.utsouthwestern.edu > Cc: Patricia Webster > Subject: [Histonet] Question > > IS there a CPT code for performing radiological exams on processed breast > cassettes for calcium? > Thanks > > Nita Searcy, HT/HTL (ASCP) > Scott and White Hospital > Division Manager, Anatomic Pathology > 2401 S. 31st. Street > 254-724-2438 > Temple, Texas, 76502 > nsearcy@swmail.sw.org > > > 254-724-2438 > > > > CONFIDENTIALITY NOTICE: The documents accompanying this email transmission > contain confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the individual > or entity named above. The authorized recipient of this information is > prohibited from disclosing this information to any other party and is > required to destroy the information after its stated need has been > fulfilled. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution, or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this email in error, please notify the sender immediately to > arrange for return of these documents. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From ECanal <@t> hei.org Tue Mar 6 15:58:52 2012 From: ECanal <@t> hei.org (Canal, Erica) Date: Fri Mar 9 13:20:57 2012 Subject: [Histonet] ammonium hydroxide Message-ID: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> Hello, I am a newly certified histotech, so I have lots to learn but I would like to know what you experts think about our tissue processing. We process mouse tissue. Tissue is fixed in nbf, and then the skull and spine is placed in decal soln. overnight while the other tissue organs etc,; are placed in 70% etoh over night for a day even two sometimes. Then when it comes to processing here is our pgm. : 70% etoh ...........15 mins 95% etoh ...........45 mins Absolute etoh....60 mins Absolute etoh ...60mins Absolute etoh....60mins Absolute etoh....60 mins Absolute etoh...60 mins Clear rite..........2hrs Clear rite .......2 hrs 15mins Clear rite.......2hrs 30 mins Histowax........1hr Histowax.........1hr Histowax........1hr 30 mins So after processing my tissues are very very dry and brittle. I soak them on ice with soapy water and sometimes use ammonium hydroxide in the ice water, to soften my tissue. I would like to know if you have any suggestions for my processing cycle and I would like to know If ammonium hydroxide affects IHC staining. Thanks much...histo newbie From trathborne <@t> somerset-healthcare.com Fri Mar 9 13:42:08 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Mar 9 13:41:53 2012 Subject: [Histonet] RE: ammonium hydroxide In-Reply-To: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> References: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FA633A@SMCMAIL01.somerset-healthcare.com> Although it has been many years since I worked in research, I never remember placing tissue in etoh overnight or longer. I would suggest that if there are any mice that are extras and don't make it into a study, that you experiment with different protocols using these mice. I'm sure you'll get more suggestions from those currently in the field. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Canal, Erica Sent: Tuesday, March 06, 2012 4:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ammonium hydroxide Hello, I am a newly certified histotech, so I have lots to learn but I would like to know what you experts think about our tissue processing. We process mouse tissue. Tissue is fixed in nbf, and then the skull and spine is placed in decal soln. overnight while the other tissue organs etc,; are placed in 70% etoh over night for a day even two sometimes. Then when it comes to processing here is our pgm. : 70% etoh ...........15 mins 95% etoh ...........45 mins Absolute etoh....60 mins Absolute etoh ...60mins Absolute etoh....60mins Absolute etoh....60 mins Absolute etoh...60 mins Clear rite..........2hrs Clear rite .......2 hrs 15mins Clear rite.......2hrs 30 mins Histowax........1hr Histowax.........1hr Histowax........1hr 30 mins So after processing my tissues are very very dry and brittle. I soak them on ice with soapy water and sometimes use ammonium hydroxide in the ice water, to soften my tissue. I would like to know if you have any suggestions for my processing cycle and I would like to know If ammonium hydroxide affects IHC staining. Thanks much...histo newbie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From aferullo <@t> celgene.com Fri Mar 9 13:52:36 2012 From: aferullo <@t> celgene.com (Andrea Ferullo (non-Celgene)) Date: Fri Mar 9 13:52:42 2012 Subject: [Histonet] RE: ammonium hydroxide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FA633A@SMCMAIL01.somerset-healthcare.com> References: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> <3AD061FE740D464FAC7BF6B5CFB7570711FA633A@SMCMAIL01.somerset-healthcare.com> Message-ID: <24701B0EF4127F4FA262751AA9674EEA029DD3E15394@SUMEXPRDMB03.celgene.com> You can try removing two of the absolute alcohol steps, as it seems you are using too much 100% etoh. Also, the tissue is probably dry from not being infiltrated properly, so instead of doing 3 paraffin steps, you can try 1 step for 1 hour to remove xylene, and then the second step over night. This should penetrate deep in the tissue and should help with the dryness. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, March 09, 2012 2:42 PM To: 'Canal, Erica'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: ammonium hydroxide Although it has been many years since I worked in research, I never remember placing tissue in etoh overnight or longer. I would suggest that if there are any mice that are extras and don't make it into a study, that you experiment with different protocols using these mice. I'm sure you'll get more suggestions from those currently in the field. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Canal, Erica Sent: Tuesday, March 06, 2012 4:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ammonium hydroxide Hello, I am a newly certified histotech, so I have lots to learn but I would like to know what you experts think about our tissue processing. We process mouse tissue. Tissue is fixed in nbf, and then the skull and spine is placed in decal soln. overnight while the other tissue organs etc,; are placed in 70% etoh over night for a day even two sometimes. Then when it comes to processing here is our pgm. : 70% etoh ...........15 mins 95% etoh ...........45 mins Absolute etoh....60 mins Absolute etoh ...60mins Absolute etoh....60mins Absolute etoh....60 mins Absolute etoh...60 mins Clear rite..........2hrs Clear rite .......2 hrs 15mins Clear rite.......2hrs 30 mins Histowax........1hr Histowax.........1hr Histowax........1hr 30 mins So after processing my tissues are very very dry and brittle. I soak them on ice with soapy water and sometimes use ammonium hydroxide in the ice water, to soften my tissue. I would like to know if you have any suggestions for my processing cycle and I would like to know If ammonium hydroxide affects IHC staining. Thanks much...histo newbie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************* THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR INDIVIDUALS NAMED ABOVE. If the reader is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please reply to the sender to notify us of the error and delete the original message. Thank You. ********************************************************* From k84as <@t> yahoo.com Fri Mar 9 13:59:34 2012 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Mar 9 13:59:38 2012 Subject: [Histonet] RE: ammonium hydroxide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FA633A@SMCMAIL01.somerset-healthcare.com> References: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> <3AD061FE740D464FAC7BF6B5CFB7570711FA633A@SMCMAIL01.somerset-healthcare.com> Message-ID: <1331323174.39168.YahooMailNeo@web140402.mail.bf1.yahoo.com> From? my experience it is better to fix all the samples in NBF to coagulate the prteins and keep the normal morphology... then we could transfer the samples to 70% ethanol and no matter how long it will stay as it is considered a fixtave. In your case the proplem is that the processing time is too long in absolute?eth. changes as it cause dryness of tissue and become difficulte to cut. The over all time in absolute eth. in our lab not exceed 80 mins. for mouse small samples. Also i recommed that you?begain with 70% then 80% then 95% followed by absolute changes and the rest of your program ? hope that help ? Mohamed Abd El razik Ass. Lec. of Cytology & Histology Faculty of Vet. Med. cairo University- Egypt ________________________________ From: "Rathborne, Toni" To: "'Canal, Erica'" ; "histonet@lists.utsouthwestern.edu" Sent: Friday, March 9, 2012 9:42 PM Subject: [Histonet] RE: ammonium hydroxide Although it has been many years since I worked in research, I never remember placing tissue in etoh overnight or longer. I would suggest that if there are any mice that are extras and don't make it into a study, that you experiment with different protocols using these mice. I'm sure you'll get more suggestions from those currently in the field. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Canal, Erica Sent: Tuesday, March 06, 2012 4:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ammonium hydroxide Hello, I am a newly certified histotech, so I have lots to learn but I would like to know what you experts think about our tissue processing. We process mouse tissue.? Tissue is fixed in nbf, and then the skull and spine is placed in decal soln. overnight while the other tissue organs etc,; are placed in 70% etoh over night for a day even two sometimes. Then when it comes to processing here is our pgm. : 70% etoh ...........15 mins 95% etoh ...........45 mins Absolute etoh....60 mins Absolute etoh ...60mins Absolute etoh....60mins Absolute etoh....60 mins Absolute etoh...60 mins Clear rite..........2hrs Clear rite .......2 hrs 15mins Clear rite.......2hrs 30 mins Histowax........1hr Histowax.........1hr Histowax........1hr 30 mins So after processing my tissues are very very dry and brittle. I soak them on ice with soapy water and sometimes use ammonium hydroxide in the ice water, to soften my tissue. I would like to know if you have any suggestions for my processing cycle and I would like to know If ammonium hydroxide affects IHC staining. Thanks much...histo newbie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Mar 9 14:21:00 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Mar 9 14:21:05 2012 Subject: [Histonet] RE: ammonium hydroxide - long response In-Reply-To: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC596C@SBS2K8.premierlab.local> Erica What kind of decal are you using? we use 10% Formic Acid, and I don't believe that a mouse skull or spine would decal properly over night unless it's a HCL acid based decal solution. So the issue may be that your samples are not adequately decaled to begin with. If you are processing soft tissue on this cycle its way too long. Here is an example of our processing cycle for mouse soft tissue and we would have a separate processing cycle for the mouse bone samples. In my opinion the samples need to processed separately, we also process mouse skin on the longer processing cycle too. For rodent soft tissue I find that after the tissue is grossed in it must be placed back into 10% NBF. If you gross and place the tissue in 70% alcohol you are going to run into problems with sectioning and the tissue will crack once the slides are stained. For soft tissue here is our processing cycle, its basically 20 minutes per station for mouse soft tissue and 30 minutes per station for rat soft tissue. If you are using a xylene substitute you will need 3 changes of that instead of 2 changes of xylene, we are now using propar for most of our rodent soft tissue samples. For mouse and rat eye samples we do not let the samples sit on the processor overnight we start the processor in the morning we use the 20 minutes cycle for both mouse and rat eyes. In the research setting I find that we will create a specific processing cycle for some tissue types and we have over 12 different cycles that we use routinely. I know of some labs that have over 30 different processing cycles. 50% alcohol 20 min 50% alcohol 20 min 70% alcohol 20 min 70% alcohol 20 min 80% alcohol 20 min 80% alcohol 20 min 95% alcohol 20 min 95% alcohol 20 min 100% alcohol 20 min 100% alcohol 20 min 100% alcohol 20 min 100% alcohol 20 min Propar 20 min Xylene 20 min Propar 20 min Xylene 20 min Propar 20 min Paraffin 20 min Paraffin 20 min Paraffin 20 min Paraffin 20 min Paraffin 20 min Paraffin 20 min Bone and Skin samples - most species For bone and skin we have found that longer processing cycles are ideal and we have not really encountered any issues with over processing, some might think that this cycle is too long but it our hands it works great. We use three absolutes and 3 xylenes and 4 paraffins for bone and skin samples for most species but we will even go longer if required for some tissue samples. 50% alcohol 60 min 70% alcohol 60 min 80% alcohol 60 min 95% alcohol 60 min 100% alcohol 60 min 100% alcohol 60 min 100% alcohol 60 min Xylene 60 min Xylene 60 min Xylene 60 min Paraffin 60 min Paraffin 60 min Paraffin 60 min Paraffin 60 min Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Canal, Erica Sent: Tuesday, March 06, 2012 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ammonium hydroxide Hello, I am a newly certified histotech, so I have lots to learn but I would like to know what you experts think about our tissue processing. We process mouse tissue. Tissue is fixed in nbf, and then the skull and spine is placed in decal soln. overnight while the other tissue organs etc,; are placed in 70% etoh over night for a day even two sometimes. Then when it comes to processing here is our pgm. : 70% etoh ...........15 mins 95% etoh ...........45 mins Absolute etoh....60 mins Absolute etoh ...60mins Absolute etoh....60mins Absolute etoh....60 mins Absolute etoh...60 mins Clear rite..........2hrs Clear rite .......2 hrs 15mins Clear rite.......2hrs 30 mins Histowax........1hr Histowax.........1hr Histowax........1hr 30 mins So after processing my tissues are very very dry and brittle. I soak them on ice with soapy water and sometimes use ammonium hydroxide in the ice water, to soften my tissue. I would like to know if you have any suggestions for my processing cycle and I would like to know If ammonium hydroxide affects IHC staining. Thanks much...histo newbie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMaslanka <@t> stpetes.org Fri Mar 9 14:31:41 2012 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Fri Mar 9 14:31:40 2012 Subject: [Histonet] Meditech Guru? Message-ID: Happy Histotechnology Professional Day everyone, Is there anyone out there using Meditech Client Server that utilizes the Pathology Module to the maximum or close to it? If so I could really use your advice. Joe Maslanka Cyto/Histo Coordinator St Peter's Laboratory Give thanks for ALL things..... From rjbuesa <@t> yahoo.com Fri Mar 9 15:00:10 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 9 15:00:15 2012 Subject: [Histonet] ammonium hydroxide In-Reply-To: <4B5D9B28C1F49548B4D3C429EE0D32E005228602@hi0sml1.hei.org> Message-ID: <1331326810.60258.YahooMailClassic@web162103.mail.bf1.yahoo.com> There are somethings I would change: 1- you have to fix all the tissues first 2- those that do require decalcification, treat them separately. Decalcify them not for a specific time period, but until they are decalcified. 3- those tissues not needing decalcification process them just after being fixed. Keeping them in EthOL70 will lead to the problem you have of being too brittle. 4- mouse tissue is very lean and dehydration should be shorter. Ideally with 2-propanol which will dehydrate more slowly and with less shrinkage. 5- I do not like clear-rite. It would be better to just use 2-propanol and substitute the first paraffin station with a mixture at equal parts of 2-propanol + paraffin (2 hours) 6- the 2 following stations with paraffin wax for 2 hours each. 7- the dehydration times you have will be good for 2-propanol but too much for EthOL 8- when the decalcified tissues are ready, process them in the same way as those not requiring decalcification. Ren? J --- On Tue, 3/6/12, Canal, Erica wrote: From: Canal, Erica Subject: [Histonet] ammonium hydroxide To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 6, 2012, 4:58 PM Hello, I am a newly certified histotech, so I have lots to learn but I would like to know what you experts think about our tissue processing. We process mouse tissue.? Tissue is fixed in nbf, and then the skull and spine is placed in decal soln. overnight while the other tissue organs etc,; are placed in 70% etoh over night for a day even two sometimes. Then when it comes to processing here is our pgm. : 70% etoh ...........15 mins 95% etoh ...........45 mins Absolute etoh....60 mins Absolute etoh ...60mins Absolute etoh....60mins Absolute etoh....60 mins Absolute etoh...60 mins Clear rite..........2hrs Clear rite .......2 hrs 15mins Clear rite.......2hrs 30 mins Histowax........1hr Histowax.........1hr Histowax........1hr 30 mins So after processing my tissues are very very dry and brittle. I soak them on ice with soapy water and sometimes use ammonium hydroxide in the ice water, to soften my tissue. I would like to know if you have any suggestions for my processing cycle and I would like to know If ammonium hydroxide affects IHC staining. Thanks much...histo newbie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From njohnson <@t> kcskincenter.com Fri Mar 9 16:38:58 2012 From: njohnson <@t> kcskincenter.com (Nacaela Johnson) Date: Fri Mar 9 16:39:05 2012 Subject: [Histonet] Happy Histotechnology Professionals Day! Message-ID: <003901ccfe45$6c4d3720$44e7a560$@com> I hope you all have a great Histotechnology Professionals Day tomorrow! Nacaela Johnson, B.S. HTL (ASCP) Histology Supervisor Kansas City Skin & Cancer Center, LLC 5810 NW Barry Rd, Ste 100 Kansas City, MO 64154 ph: 816 584 8100 fx: 816 584 8106 em: njohnson@kcskincenter.com From tigger72837 <@t> yahoo.com Fri Mar 9 19:05:32 2012 From: tigger72837 <@t> yahoo.com (Lawana Moore) Date: Fri Mar 9 19:05:32 2012 Subject: [Histonet] H&E Message-ID: I have been using surgipath hematoxylin for years. Now for some reason (that I can't figure out!!) it is faded and turning gray. I stain about 200 slides a day. Please any help is welcome. I'm ready to try anything. It's making me crazy. Thank You Lawana Moore Styles Dermatopathology Sent from my iPad From POWELL_SA <@t> mercer.edu Fri Mar 9 21:12:16 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Mar 9 21:12:26 2012 Subject: [Histonet] GSH-Region III meeting Callaway Gardens GA Message-ID: <1A9D499290EAC14F835C7004311B09CF268A7908AB@MERCERMAIL.MercerU.local> Hi everyone, I want to remind you of the Georgia Society for Histotechnology hosting Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,. Three important points: 1. PLEASE RESERVE YOUR ROOM BEFORE MARCH 13TH, AS WE HAVE ADDED ANOTHER BLOCK OF ROOMS~ We exceeded the number of rooms blocked and have added another block. The Deadline for releasing these rooms and for receiving the discounted hotel rate is March 13th, so act now. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here in Georgia. Make reservations now at 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program which can be found at http://www.histosearch.com/gsh/symposium.html. 2. PLEASE REGISTER NOW AS "AWAITING FUNDS" if you are waiting approval for the meeting go ahead and register for your workshops, luncheon, dinner and note on the form that you are awaiting funds below the total line. When approved, then send the funds, or if time does not allow, pay on site. But please register as soon as possible, don?t wait. 3. We are excited to have 23 vendors exhibiting at our meeting. They are: BioCare ~Sponsor of new IHC Award BioGenex B/R Instruments Cancer Diagnostics Cell Marque Clarient~ sponsoring dinner at Dagher's Dako EMS Epitomics General Data IMEB Lab Storage Leica Microsystems ~ sponsoring dinner at Dagher?s Leica Biosystems Mopec PolyScientific R&D Sakura ? Sponsor of GSH Histotechnologist of the Year Award hermoFisher TBS StatLab Ventana Not attending but supporting GSH are: Newcomer ? Sponsoring a Break and provided totes CL Sturkey ? Door prizes Come experience?????????????. Histotechnology ? Southern Style Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From c_m_hernandezjr <@t> yahoo.com Fri Mar 9 21:55:58 2012 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Fri Mar 9 21:56:05 2012 Subject: [Histonet] Histology Consulting Message-ID: <553BC9EB-7543-4957-886B-40207077521F@yahoo.com> Does anyone have any ideas on what the going rates are for Consultants starting up in office labs? Duties would include design, contact for construction team, ordering and setting up equipment, setting up accounts for supplies and consumables, creating all manuals and logs including CLIA manual, and hiring histotech. All help greatly appreciated! Carlos From c_m_hernandezjr <@t> yahoo.com Fri Mar 9 22:29:41 2012 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Fri Mar 9 22:29:47 2012 Subject: [Histonet] Histology Consulting Message-ID: <72381D42-F4F8-455F-8BE1-A0BEDF823F44@yahoo.com> Colorado and Wyoming for now. Carlos From melissa <@t> alliedsearchpartners.com Sat Mar 10 06:41:14 2012 From: melissa <@t> alliedsearchpartners.com (Melissa) Date: Sat Mar 10 06:41:17 2012 Subject: [Histonet] Happy Histotechnology Professionals Day From ASP! Message-ID: Good Morning to all! I just want to say Happy Histotechnology Professionals day to everyone working in the histology field. I hope you have a great day. THANK YOU to each and every one of you for working hard behind the scenes without ceasing and with great compassion for the patient and patient care! Have a great weekend! Melissa Phelan, President, Laboratory Staffing LinkedIn: http://www.linkedin.com/in/melissaphelan Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 C: 407.697.1175 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 The email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From histotalk <@t> yahoo.com Sat Mar 10 07:54:56 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Mar 10 07:54:59 2012 Subject: [Histonet] Happy HPD! Message-ID: <1331387696.40986.YahooMailNeo@web120606.mail.ne1.yahoo.com> Hi Everyone - Happy HISTOTECHNOLOGY PROFESSIONALS DAY! ? Yours, David From rjbuesa <@t> yahoo.com Sat Mar 10 09:38:40 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 10 09:38:44 2012 Subject: [Histonet] H&E In-Reply-To: Message-ID: <1331393920.46553.YahooMailClassic@web162102.mail.bf1.yahoo.com> Contact your provider and try to find out. Ren? J. --- On Fri, 3/9/12, Lawana Moore wrote: From: Lawana Moore Subject: [Histonet] H&E To: "histonet@lists.utsouthwestern.edu" Date: Friday, March 9, 2012, 8:05 PM I have been using surgipath hematoxylin for years. Now for some reason (that I can't figure out!!) it is faded and turning gray. I stain about 200 slides a day. Please any help is welcome. I'm ready to try anything. It's making me crazy. Thank You Lawana Moore Styles Dermatopathology Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Mar 10 09:40:53 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 10 09:40:58 2012 Subject: [Histonet] Histology Consulting In-Reply-To: <553BC9EB-7543-4957-886B-40207077521F@yahoo.com> Message-ID: <1331394053.41683.YahooMailClassic@web162101.mail.bf1.yahoo.com> As in any aspect of histology, there are no rates and each consultant may have his/her own scale. Seek a good consultant, ask directly and decide if you want to contract or not. Ren? J. --- On Fri, 3/9/12, Carlos Hernandez wrote: From: Carlos Hernandez Subject: [Histonet] Histology Consulting To: "Histonet" Date: Friday, March 9, 2012, 10:55 PM Does anyone have any ideas on what the going rates are for Consultants starting up in office labs? Duties would include design, contact for construction team, ordering and setting up equipment, setting up accounts for supplies and consumables, creating all manuals and logs including CLIA manual, and hiring histotech. All help greatly appreciated! Carlos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhartologist <@t> gmail.com Sat Mar 10 22:03:02 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sat Mar 10 22:03:06 2012 Subject: [Histonet] Thick Sections cut at 8 microns and up Message-ID: Hello Hitsonetters! I worked on a lab today for my Histology program which called for cutting brain sections at 12 microns. The lab we are doing is Weils to demonstrate myelin. Man oh man was it hard to cut 12 micron sections. Apparently 10 microns work for this stain as well which I managed to do that more easily than 12 but I used the idea of using a small brush to help guide the section down the knife plate like when cutting frozen sections so it wouldn't instantly roll up into a tube which is what the 12 micron sections kept doing. Any tricks to cutting thick sections? Suggestions for the student here? Thanks. -Bharti Parihar From Barry.R.Rittman <@t> uth.tmc.edu Sun Mar 11 06:47:56 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sun Mar 11 06:52:33 2012 Subject: [Histonet] Thick Sections cut at 8 microns and up In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9179C9F7386@UTHCMS1.uthouston.edu> Bharti One thing that usually works is to have a thin layer of a softer melting point wax on the top and bottom of the block, this should allow the sections to adhere to each other and not roll up. Need only a 5 degree or so difference in the wax for this to work. Another is to use a slightly lower melting point wax for your embedding. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bharti Parihar [bhartologist@gmail.com] Sent: Saturday, March 10, 2012 10:03 PM To: Histonet Archive Subject: [Histonet] Thick Sections cut at 8 microns and up Hello Hitsonetters! I worked on a lab today for my Histology program which called for cutting brain sections at 12 microns. The lab we are doing is Weils to demonstrate myelin. Man oh man was it hard to cut 12 micron sections. Apparently 10 microns work for this stain as well which I managed to do that more easily than 12 but I used the idea of using a small brush to help guide the section down the knife plate like when cutting frozen sections so it wouldn't instantly roll up into a tube which is what the 12 micron sections kept doing. Any tricks to cutting thick sections? Suggestions for the student here? Thanks. -Bharti Parihar _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Mar 11 08:49:57 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Mar 11 08:50:02 2012 Subject: [Histonet] false positive CD31? Message-ID: <7AF579FF12924F39BEA31D1AA7C1F877@dielangs.at> Dear histonet! Has anyone seen granular, fine dot-like, cytoplasmic CD-31 staining in tumorcells, that was thought to be false positive? It looks like really specific staining, but my pathologist sent this case to an expert, who couldn't reproduce this staining. The expert is really an expert and his diagnosis is not to be bothered. We stain with dako-ab on benchmark Ultra (CC1 mild, 32min plus amplifier, 1:50). thank you Gudrun Lang Biomed. Analytikerin histolab, Linz, Austria From rjbuesa <@t> yahoo.com Sun Mar 11 10:51:47 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 11 10:51:52 2012 Subject: Fw: Re: [Histonet] Antibody diluent Message-ID: <1331481107.25101.YahooMailClassic@web162104.mail.bf1.yahoo.com> --- On Sun, 3/11/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Antibody diluent To: "Dr. Mohammad Golam Mostafa" Date: Sunday, March 11, 2012, 11:13 AM Yes, there will be a problem, namely that the antibodies (proteins) need a diluted protein base to "float", and a preservative to prevent bacterial growth. Under separate cover I am sending you my recipe to prepare the antibody diluent "in house". Ren? J. --- On Sat, 3/10/12, Dr. Mohammad Golam Mostafa wrote: From: Dr. Mohammad Golam Mostafa Subject: [Histonet] Antibody diluent To: rjbuesa@yahoo.com Date: Saturday, March 10, 2012, 1:20 PM I am a Pathologist of Bangladesh. Is there any problem, if I use TBS as antibody diluents? Please let me know. ? Dr. Mohammad Golam Mostafa Dhaka, Bangladesh ? From dbeil1 <@t> hotmail.com Sun Mar 11 12:35:19 2012 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Sun Mar 11 12:35:25 2012 Subject: [Histonet] Unsubscribe Message-ID: Please unsubscribe. Damaris E. Beil "Live Well, Laugh Often, Love Much" From tuyenmai77 <@t> yahoo.com Sun Mar 11 15:40:48 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Sun Mar 11 15:40:51 2012 Subject: [Histonet] (no subject) Message-ID: <1331498448.85453.yint-ygo-j2me@web162801.mail.bf1.yahoo.com> Greetings, friend! http://nahku3m.zymichost.com/party.php?cytjs=59&hosyzapi=165&ilaze=56 From fbozkurt <@t> gmail.com Mon Mar 12 05:23:51 2012 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Mon Mar 12 05:23:58 2012 Subject: [Histonet] false positive CD31? In-Reply-To: <7AF579FF12924F39BEA31D1AA7C1F877@dielangs.at> References: <7AF579FF12924F39BEA31D1AA7C1F877@dielangs.at> Message-ID: Hello, if you send images, I can say something. And I also can send cd31 positive images from different vascular tumors. On Sun, Mar 11, 2012 at 3:49 PM, Gudrun Lang wrote: > Dear histonet! > > > > Has anyone seen granular, fine dot-like, cytoplasmic CD-31 staining in > tumorcells, that was thought to be false positive? > > It looks like really specific staining, but my pathologist sent this case > to > an expert, who couldn't reproduce this staining. > > The expert is really an expert and his diagnosis is not to be bothered. > > > > We stain with dako-ab on benchmark Ultra (CC1 mild, 32min plus amplifier, > 1:50). > > > > thank you > > Gudrun Lang > > > > Biomed. Analytikerin > > histolab, Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 From TNMayer <@t> mdanderson.org Mon Mar 12 07:47:01 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Mar 12 07:47:06 2012 Subject: [Histonet] RE: Histology Consulting Message-ID: Not sure what the rates are, but remember this-negotiate at least a 1 year contract for the tech. Some POL's are hiring techs, bringing someone else in to train and then releasing the tech. Toysha Message: 11 Date: Fri, 9 Mar 2012 20:55:58 -0700 From: Carlos Hernandez Subject: [Histonet] Histology Consulting To: Histonet Message-ID: <553BC9EB-7543-4957-886B-40207077521F@yahoo.com> Content-Type: text/plain; charset=us-ascii Does anyone have any ideas on what the going rates are for Consultants starting up in office labs? Duties would include design, contact for construction team, ordering and setting up equipment, setting up accounts for supplies and consumables, creating all manuals and logs including CLIA manual, and hiring histotech. All help greatly appreciated! Carlos ------------------------------ Message: 12 Date: Fri, 9 Mar 2012 21:29:41 -0700 From: Carlos Hernandez Subject: [Histonet] Histology Consulting To: Histonet Message-ID: <72381D42-F4F8-455F-8BE1-A0BEDF823F44@yahoo.com> Content-Type: text/plain; charset=us-ascii Colorado and Wyoming for now. Carlos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Sat, 10 Mar 2012 07:40:53 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Histology Consulting To: Histonet , Carlos Hernandez Message-ID: <1331394053.41683.YahooMailClassic@web162101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 As in any aspect of histology, there are no rates and each consultant may have his/her own scale. Seek a good consultant, ask directly and decide if you want to contract or not. Ren? J. --- On Fri, 3/9/12, Carlos Hernandez wrote: From: Carlos Hernandez Subject: [Histonet] Histology Consulting To: "Histonet" Date: Friday, March 9, 2012, 10:55 PM Does anyone have any ideas on what the going rates are for Consultants starting up in office labs? Duties would include design, contact for construction team, ordering and setting up equipment, setting up accounts for supplies and consumables, creating all manuals and logs including CLIA manual, and hiring histotech. All help greatly appreciated! Carlos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 12 ***************************************** From Jeffery.Howery <@t> jcl.com Mon Mar 12 09:59:02 2012 From: Jeffery.Howery <@t> jcl.com (Jeffery Howery) Date: Mon Mar 12 09:59:09 2012 Subject: [Histonet] Undecalcified bone IHC Message-ID: Does anyone have a protocol for Undecalcified bone for IHC? From rjbuesa <@t> yahoo.com Mon Mar 12 10:06:57 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 12 10:07:04 2012 Subject: [Histonet] Undecalcified bone IHC In-Reply-To: Message-ID: <1331564817.74576.YahooMailClassic@web162101.mail.bf1.yahoo.com> Undecalcified? How are you going to section it? If you can section it, just use any IHC protocol for regular sections. Good luck! Ren? J. --- On Mon, 3/12/12, Jeffery Howery wrote: From: Jeffery Howery Subject: [Histonet] Undecalcified bone IHC To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 10:59 AM Does anyone have a protocol for Undecalcified bone for IHC? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Mon Mar 12 10:35:27 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Mar 12 10:35:37 2012 Subject: [Histonet] Undecalcified bone IHC In-Reply-To: <1331564817.74576.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1331564817.74576.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: Hi Jeff, If is it possible a few more specifics of how the tissue has been received, processed and evaluated would help. Undecalcified bone sectioning procedures vary and also what specific markers are you looking to do is important. Vikki On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa wrote: > Undecalcified? How are you going to section it? > If you can section it, just use any IHC protocol for regular sections. > Good luck! > Ren? J. > > --- On Mon, 3/12/12, Jeffery Howery wrote: > > > From: Jeffery Howery > Subject: [Histonet] Undecalcified bone IHC > To: histonet@lists.utsouthwestern.edu > Date: Monday, March 12, 2012, 10:59 AM > > > Does anyone have a protocol for Undecalcified bone for IHC? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From smcbride <@t> andrew.cmu.edu Mon Mar 12 10:50:56 2012 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Mon Mar 12 10:51:17 2012 Subject: [Histonet] Undecalcified bone IHC In-Reply-To: <1331564817.74576.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1331564817.74576.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: <7A31DAFD-2338-469E-887F-47B0B3867D97@andrew.cmu.edu> Ren? & Jeffery, I don't see sectioning as that big of a challenge. I often thin section mineralized bone sections ~7 microns with my Leica microtome. Although I have a great interest in developing an IHC technique for mineralized bone, I have never had a spare moment to carry out any developmental work. I am concerned more about the effects of the solvents used in hard tissue histology on the antigens/epitopes of interest. Does anyone use IHC staining on PMMA mineralized bone specimens on a regular basis? Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Mar 12, 2012, at 11:06 AM, Rene J Buesa wrote: > Undecalcified? How are you going to section it? > If you can section it, just use any IHC protocol for regular sections. > Good luck! > Ren? J. > > --- On Mon, 3/12/12, Jeffery Howery wrote: > > > From: Jeffery Howery > Subject: [Histonet] Undecalcified bone IHC > To: histonet@lists.utsouthwestern.edu > Date: Monday, March 12, 2012, 10:59 AM > > > Does anyone have a protocol for Undecalcified bone for IHC? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Mon Mar 12 11:06:41 2012 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Mar 12 11:07:15 2012 Subject: [Histonet] H&E In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E0956180F@LSRIEXCH1.lsmaster.lifespan.org> I have also used Surgipath hematoxylin (Gill III) for years, and am having no problems at this time. My last shipment was about 3 months ago. From gayle.callis <@t> bresnan.net Mon Mar 12 12:04:20 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Mar 12 12:04:37 2012 Subject: [Histonet] Re: undecalcified bone IHC Message-ID: <000001cd0072$2cbb8150$863283f0$@bresnan.net> Jeff, It is most certainly possible to do IHC on undecalcifed bone sections embedded in PMMA although not the easiest task. Sectioning is done on a microtome that is powerful enough to cut the plastic and using tungsten carbide knives. The key is total removal of the plastic from MMA embedded bone sections to allow antibody/ immunoglobulins to access antigenic sites. Neil Hand has done IHC successfully on PMMA embedded tissues including undecalcified bone on 2 to 3 ?m thick sections. I think one could cut thicker sections at 4 to 5 ?m and still be successful. I do not recall what Troiano et al used. The following publications will help you and should include protocols, although conventional protocols will work according to Hand. Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl methacrylate resin for embedding bone marrow trephine biopsies. Hand NM et al 1996 Antigen unmasking using microwave heating on formalin fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 Jackson P et al. 1996 Amplification of immunocytochemical reactions by the catalytic deposition of biotin on tissue sections. J Path 170(suppl):23A. This was about tyramide amplification when one gets a weak signal from "conventional" methods. Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and application in unmasking antigens embedded in methyl methacrylate. J Histotechnology 2`:231-236 Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light microscopy: a novel post embedding procedure. Proceeding Royal Microscopical Society 24(1):A54-55. Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory and Practice of Histological Technique, 5th edition by Gamble and Bancroft. The 6th edition is updated under same title. Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA embedded bone sections with publications in J Histotechnology. Hand mentioned several HIER methods, using citrate buffer. Optimizing retrieval will depend on the antigen and you may end up doing this with some form of HIER, including microwave or other heat producing methods and with different buffers. Enzyme digestion is also a possibility. Hand removed MMA with xylene, warm my speed up the removal, also more than one change for 10 - 20 minutes or longer. When I talked to him personally, he said he had used warm xylene although temperature was not mentioned in his chapter. After MMA removal, rehydrate section through alcohol gradient as one does paraffin sections. He was emphatic about never allowing the sections dry out. Hopefully Jack Ratliff and Damien Laudier will provide more insight on this topic. Good luck Gayle M. Callis HTL/HT/MT(ASCP) ****************************************** Hi Jeff, If is it possible a few more specifics of how the tissue has been received, processed and evaluated would help. Undecalcified bone sectioning procedures vary and also what specific markers are you looking to do is important. Vikki On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> wrote: > Undecalcified? How are you going to section it? > If you can section it, just use any IHC protocol for regular sections. > Good luck! > Ren? J. > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > From: Jeffery Howery jcl.com> > Subject: [Histonet] Undecalcified bone IHC > To: histonet <@t> lists.utsouthwestern.edu > Date: Monday, March 12, 2012, 10:59 AM > > > Does anyone have a protocol for Undecalcified bone for IHC? > From bakevictoria <@t> gmail.com Mon Mar 12 12:09:39 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Mar 12 12:09:48 2012 Subject: [Histonet] Re: undecalcified bone IHC In-Reply-To: <000001cd0072$2cbb8150$863283f0$@bresnan.net> References: <000001cd0072$2cbb8150$863283f0$@bresnan.net> Message-ID: Thank you Gayle. Vikki On Mar 12, 2012 1:04 PM, "gayle callis" wrote: > Jeff, > > > > It is most certainly possible to do IHC on undecalcifed bone sections > embedded in PMMA although not the easiest task. Sectioning is done on a > microtome that is powerful enough to cut the plastic and using tungsten > carbide knives. The key is total removal of the plastic from MMA embedded > bone sections to allow antibody/ immunoglobulins to access antigenic sites. > Neil Hand has done IHC successfully on PMMA embedded tissues including > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > thicker sections at 4 to 5 ?m and still be successful. I do not recall > what > Troiano et al used. > > > > The following publications will help you and should include protocols, > although conventional protocols will work according to Hand. > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > methacrylate resin for embedding bone marrow trephine biopsies. > > Hand NM et al 1996 Antigen unmasking using microwave heating on formalin > fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > the catalytic deposition of biotin on tissue sections. J Path > 170(suppl):23A. This was about tyramide amplification when one gets a weak > signal from "conventional" methods. > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and > application in unmasking antigens embedded in methyl methacrylate. J > Histotechnology 2`:231-236 > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light > microscopy: a novel post embedding procedure. Proceeding Royal > Microscopical Society 24(1):A54-55. > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory > and Practice of Histological Technique, 5th edition by Gamble and > Bancroft. > The 6th edition is updated under same title. > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA > embedded bone sections with publications in J Histotechnology. > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > retrieval will depend on the antigen and you may end up doing this with > some > form of HIER, including microwave or other heat producing methods and with > different buffers. Enzyme digestion is also a possibility. > > > > Hand removed MMA with xylene, warm my speed up the removal, also more than > one change for 10 - 20 minutes or longer. When I talked to him > personally, > he said he had used warm xylene although temperature was not mentioned in > his chapter. After MMA removal, rehydrate section through alcohol > gradient > as one does paraffin sections. He was emphatic about never allowing the > sections dry out. > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on this > topic. > > > > Good luck > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > ****************************************** > > > > Hi Jeff, > > > > If is it possible a few more specifics of how the tissue has been received, > > processed and evaluated would help. Undecalcified bone sectioning > > procedures vary and also what specific markers are you looking to do is > > important. > > > > Vikki > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> > wrote: > > > > > Undecalcified? How are you going to section it? > > > If you can section it, just use any IHC protocol for regular sections. > > > Good luck! > > > Ren? J. > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > > > > > > > From: Jeffery Howery jcl.com> > > > Subject: [Histonet] Undecalcified bone IHC > > > To: histonet <@t> lists.utsouthwestern.edu > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From NKonop <@t> chw.org Mon Mar 12 12:35:07 2012 From: NKonop <@t> chw.org (Konop, Nicole) Date: Mon Mar 12 12:35:18 2012 Subject: [Histonet] Unsubscribe Message-ID: Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department From Stacy.Giroux <@t> stjohn.org Mon Mar 12 12:35:47 2012 From: Stacy.Giroux <@t> stjohn.org (Giroux, Stacy) Date: Mon Mar 12 12:35:53 2012 Subject: [Histonet] Gram Stain Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From Friedrich_Hahn <@t> bd.com Mon Mar 12 13:31:44 2012 From: Friedrich_Hahn <@t> bd.com (Friedrich_Hahn@bd.com) Date: Mon Mar 12 13:31:58 2012 Subject: [Histonet] DAKO Autostainer Issues Message-ID: We have been having issues with our Autostainer since bringing it out of storage. All parts are in excellent shape, according to the technician that performed the PM. It worked fine for a month of so, but then began malfunctioning after only a couple of staining runs. At some point in the staining process, the machine appears to lose its XY homing and jams itself against the front right corner, dispensing buffer onto the floor of the chamber and occasionally making grinding noises. We've had a technician take a look at it, 'clean' the software, and we thought we had it resolved, but the problem appears to be back. Has anyone experienced this issue? If so, was it hardware- or software- based? Thanks in advance! ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* From Laurie <@t> blufrogpath.com Mon Mar 12 13:34:20 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Mon Mar 12 13:34:29 2012 Subject: [Histonet] Color Blindness Testing Message-ID: <20120312113420.295dc6182df7e5cbb4f32bc101c30dcc.f82e5fb846.wbe@email15.secureserver.net> Does anyone know of any (free) online testing for color blindness Does anyone have an alternate method that they have used to satisfy CAP requirements? Laurie From 41dmb41 <@t> gmail.com Mon Mar 12 13:42:44 2012 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Mar 12 13:42:55 2012 Subject: [Histonet] DAKO Autostainer Issues In-Reply-To: References: Message-ID: <5FAEEDF0-63D7-4EBC-9A3E-AEF9D5297130@gmail.com> There's a circuit board on the arm near the barcode scanner that controls the movement and orientation of the arm. If that circuitboard malfunctions, then you can see some of the erratic behavior that you are describing. That's one possibility I can think of that might cause that kind of problem. However, the technician who looked at it should be able to troubleshoot and replace that board if it is a problem. Drew Sent from my iPhone On Mar 12, 2012, at 2:31 PM, Friedrich_Hahn@bd.com wrote: > > We have been having issues with our Autostainer since bringing it out > of storage. All parts are in excellent shape, according to the > technician that performed the PM. It worked fine for a month of so, > but then began malfunctioning after only a couple of staining runs. > At some point in the staining process, the machine appears to lose its > XY homing and jams itself against the front right corner, dispensing > buffer onto the floor of the chamber and occasionally making grinding > noises. We've had a technician take a look at it, 'clean' the > software, and we thought we had it resolved, but the problem appears > to be back. > Has anyone experienced this issue? If so, was it hardware- or > software- based? > Thanks in advance! > > ----------------------------------------- > ******************************************************************* > IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may > constitute an advertisement of a BD group's products or services or a > solicitation of interest in them. If this is such a message and you > would like to opt out of receiving future advertisements or > solicitations from this BD group, please forward this e-mail to > optoutbygroup@bd.com. > ******************************************************************* > This message (which includes any attachments) is intended only for the > designated recipient(s). It may contain confidential or proprietary > information and may be subject to the attorney-client privilege or > other confidentiality protections. If you are not a designated > recipient, you may not review, use, copy or distribute this message. > If you received this in error, please notify the sender by reply > e-mail and delete this message. Thank you. > ******************************************************************* > Corporate Headquarters Mailing Address: BD (Becton, Dickinson and > Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. > ******************************************************************* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeffery.Howery <@t> jcl.com Mon Mar 12 13:49:10 2012 From: Jeffery.Howery <@t> jcl.com (Jeffery Howery) Date: Mon Mar 12 13:49:22 2012 Subject: [Histonet] IHC for decalcified bone protocol Message-ID: Anyone have a Protocol for Decalcified bone for IHC? From fbozkurt <@t> gmail.com Mon Mar 12 13:51:26 2012 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Mon Mar 12 13:51:32 2012 Subject: [Histonet] IHC for decalcified bone protocol In-Reply-To: References: Message-ID: cool !! On Mon, Mar 12, 2012 at 8:49 PM, Jeffery Howery wrote: > Anyone have a Protocol for Decalcified bone for IHC? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 From gayle.callis <@t> bresnan.net Mon Mar 12 14:08:55 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Mar 12 14:09:09 2012 Subject: [Histonet] Gram Stain In-Reply-To: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> References: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> Message-ID: <000001cd0083$93bf5c80$bb3e1580$@bresnan.net> Stacy, You can buy a complete Brown and Brenn staining kit from Newcomer Supply which is very good, and not change your method. The picric acid/acetone mixture is included and you wouldn't have to store stock picric acid nor ether in the lab but just this small amount of Picric acid/acetone that comes in the kit. Disposal should not be that much of a problem. They also have a Hucker Twort Gram stain that uses acetone for destaining and you supply the acetone. Poly Scientific has Gram Stain kit (Hucker modification) which uses Grams decolorizer, a mixture of acetone and alcohol. They have excellent staining kits too. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Monday, March 12, 2012 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Courtney.Pierce <@t> quintiles.com Mon Mar 12 14:35:48 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Mon Mar 12 14:35:58 2012 Subject: [Histonet] IHC Slides Message-ID: Having problems with IHC slides not looking the same between runs. Does it have to do with how long and at what temp they are in the oven for? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From rjbuesa <@t> yahoo.com Mon Mar 12 14:52:12 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 12 14:52:16 2012 Subject: [Histonet] IHC Slides In-Reply-To: Message-ID: <1331581932.14770.YahooMailClassic@web162101.mail.bf1.yahoo.com> Not really. Time in the oven and temp is usually not an issue as long as the tissue is well fixed/processed/infiltrated. Ren? J. --- On Mon, 3/12/12, Courtney Pierce wrote: From: Courtney Pierce Subject: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 12, 2012, 3:35 PM Having problems with IHC slides not looking the same between runs. Does it have to do with how long and at what temp they are in the oven for? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital **********************? IMPORTANT--PLEASE READ? ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information.? If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited.? If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Mar 12 18:38:11 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Mar 12 18:38:23 2012 Subject: [Histonet] RE: Gram Stain In-Reply-To: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> References: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A20038@xmdb02.nch.kids> Stacy, There are several Gram stain variants that do not use Picric acid. Tworts variant uses fast green and neutral red after the crystal violet-Iodine steps. Brown-Hopps method uses Basic Fuchsin, Gallego's differentiator and 1.5% Tartrazine after the crystal-violet steps, to stain the gram negative bugs. A simple red counterstain is to stain sections with 1% aqueous neutral red for 3 min after the crystal violet-Iodine steps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Tuesday, 13 March 2012 4:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From chak_bou <@t> yahoo.com Mon Mar 12 20:19:14 2012 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Mon Mar 12 20:19:17 2012 Subject: [Histonet] CD34 Message-ID: <1331601554.30627.YahooMailClassic@web161804.mail.bf1.yahoo.com> Hello histonet, Does anyone using CD34? if so, can you share the staining protocol with us? and what are you using for antigen retrieval? Thank you so much. Chakib Boussahmain HTL(ASCP) From enrriq88 <@t> yahoo.com Mon Mar 12 20:25:44 2012 From: enrriq88 <@t> yahoo.com (enrriq88@yahoo.com) Date: Mon Mar 12 20:25:48 2012 Subject: [Histonet] (no subject) Message-ID: <1331601944.56861.YahooMailNeo@web113503.mail.gq1.yahoo.com> Please?unsubscribe. thanks From adesupo2002 <@t> hotmail.com Mon Mar 12 20:26:20 2012 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Mon Mar 12 20:26:24 2012 Subject: [Histonet] Liver Biopsy Processing Schedule Message-ID: Hi, I was wondering if anyone can share their LIVER Biopsy Processing Schedule with me? Thanks, Ade From a.thotakura <@t> imperial.ac.uk Tue Mar 13 06:06:01 2012 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Tue Mar 13 06:06:16 2012 Subject: [Histonet] CD45.1 & CD45.2 IF on parrafin sections. Message-ID: Dear All, I want to do immunofluorescence on formalin fixed paraffin sections, Can you guys help me sending protocol how to do it ? Thank you very much for your help. BW Anil From bliven.laura <@t> marshfieldclinic.org Tue Mar 13 08:29:17 2012 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Tue Mar 13 08:29:41 2012 Subject: [Histonet] IHC Processor Validation & MUM1 Message-ID: <201203131329.q2DDTXa8031218@mailhost3.mfldclin.edu> 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From histogirl4 <@t> gmail.com Tue Mar 13 08:54:27 2012 From: histogirl4 <@t> gmail.com (Sabrina Townsend) Date: Tue Mar 13 08:54:43 2012 Subject: [Histonet] Ihc validation Message-ID: <27E73F93-AB37-4260-8BEA-35F23AF4F022@gmail.com> I am having to validate antibodies and was wondering if you have to do a parallel study or just use known positive and negative tissue? ~Sabrina~ From ratliffjack <@t> hotmail.com Tue Mar 13 09:50:23 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Mar 13 09:50:33 2012 Subject: [Histonet] Re: undecalcified bone IHC In-Reply-To: <000001cd0072$2cbb8150$863283f0$@bresnan.net> References: <000001cd0072$2cbb8150$863283f0$@bresnan.net> Message-ID: I might also add that Neil Hand is co-speaking with myself and Philip Seifert this year at the annual National Society for Histotechnology - Symposium/Convention in Vancouver B.C. Our workshop is titled: Resin Applications Forum: Methods for Processing, Special Staining, Immunohistochemical and In Situ Hybridization of Soft and Hard Tissue Including Medical Device Implants During the last 50 years, numerous histological procedures have been described on resin embedded tissue. While different types of resins are available for different purposes, the acrylics provide the widest range of techniques, especially for light microscopy applications. However, as demand from H&E to more sophisticated techniques increases, so too have the problems, and nowhere is this more apparent and controversial than in the application of immunohistochemistry on resin sections. This workshop will provide a review and discussion for those individuals that currently work with and/or are just getting started working with soft and hard tissue specimens and specifically the various resins (i.e. MMA, GMA, Technovit, Acrylosin, etc.) associated with their specific tissue interests. The workshop will also detail the preparation and staining of sections of soft and hard tissue, including implants (e.g. undemineralized bone and cardiovascular stents), for immunohistochemical and in situ hybridization staining using different acrylic and epoxy resin embedding media. Specific problems and pitfalls, either technical or operational associated with certain resin embedding procedures, will be illustrated and examined. Particular emphasis will be given to procedures which have been used extensively for routine diagnostic, and research purposes, i.e. those that WORK! Individuals with a current or future intent to process and cut undemineralized tissue or tissue containing foreign implant materials using acrylic or epoxy resins are strongly encouraged to attend this workshop! Please feel free to contact me if you would like more information about the workshop as information relevant to the exact date and time becomes available. All I know at this time is that the NSH meeting is September 29th - October 3rd, 2012. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology > From: gayle.callis@bresnan.net > To: histonet@lists.utsouthwestern.edu > Date: Mon, 12 Mar 2012 11:04:20 -0600 > Subject: [Histonet] Re: undecalcified bone IHC > > Jeff, > > > > It is most certainly possible to do IHC on undecalcifed bone sections > embedded in PMMA although not the easiest task. Sectioning is done on a > microtome that is powerful enough to cut the plastic and using tungsten > carbide knives. The key is total removal of the plastic from MMA embedded > bone sections to allow antibody/ immunoglobulins to access antigenic sites. > Neil Hand has done IHC successfully on PMMA embedded tissues including > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > thicker sections at 4 to 5 ?m and still be successful. I do not recall what > Troiano et al used. > > > > The following publications will help you and should include protocols, > although conventional protocols will work according to Hand. > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > methacrylate resin for embedding bone marrow trephine biopsies. > > Hand NM et al 1996 Antigen unmasking using microwave heating on formalin > fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > the catalytic deposition of biotin on tissue sections. J Path > 170(suppl):23A. This was about tyramide amplification when one gets a weak > signal from "conventional" methods. > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and > application in unmasking antigens embedded in methyl methacrylate. J > Histotechnology 2`:231-236 > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light > microscopy: a novel post embedding procedure. Proceeding Royal > Microscopical Society 24(1):A54-55. > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory > and Practice of Histological Technique, 5th edition by Gamble and Bancroft. > The 6th edition is updated under same title. > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA > embedded bone sections with publications in J Histotechnology. > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > retrieval will depend on the antigen and you may end up doing this with some > form of HIER, including microwave or other heat producing methods and with > different buffers. Enzyme digestion is also a possibility. > > > > Hand removed MMA with xylene, warm my speed up the removal, also more than > one change for 10 - 20 minutes or longer. When I talked to him personally, > he said he had used warm xylene although temperature was not mentioned in > his chapter. After MMA removal, rehydrate section through alcohol gradient > as one does paraffin sections. He was emphatic about never allowing the > sections dry out. > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on this > topic. > > > > Good luck > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > ****************************************** > > > > Hi Jeff, > > > > If is it possible a few more specifics of how the tissue has been received, > > processed and evaluated would help. Undecalcified bone sectioning > > procedures vary and also what specific markers are you looking to do is > > important. > > > > Vikki > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> > wrote: > > > > > Undecalcified? How are you going to section it? > > > If you can section it, just use any IHC protocol for regular sections. > > > Good luck! > > > Ren? J. > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > > > > > > > From: Jeffery Howery jcl.com> > > > Subject: [Histonet] Undecalcified bone IHC > > > To: histonet <@t> lists.utsouthwestern.edu > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 13 10:06:35 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 13 10:06:43 2012 Subject: [Histonet] CD34 In-Reply-To: <1331601554.30627.YahooMailClassic@web161804.mail.bf1.yahoo.com> Message-ID: <1331651195.30522.YahooMailClassic@web162106.mail.bf1.yahoo.com> Becton-Dickinson Monoclonal antibody at 1:100 for 30 minutes after HIER (citrate at pH6) with placenta as control. Ren? J. --- On Mon, 3/12/12, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 9:19 PM Hello histonet, Does anyone using CD34? if so, can you share the staining protocol with us? and what are you using for antigen retrieval? Thank you so much. Chakib Boussahmain HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 13 10:25:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 13 10:25:59 2012 Subject: [Histonet] IHC Processor Validation & MUM1 In-Reply-To: <201203131329.q2DDTXa8031218@mailhost3.mfldclin.edu> Message-ID: <1331652350.10883.YahooMailClassic@web162101.mail.bf1.yahoo.com> CAP has some guidelines as to the number of specimens and antibodies to test. I think to remember that the lowest amount was 25 specimens, but I do not remember about the antibodies but it makes sense to use only those your use to work with. Ren? J. --- On Tue, 3/13/12, Bliven, Laura wrote: From: Bliven, Laura Subject: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 13, 2012, 9:29 AM 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sruby <@t> 4path.com Tue Mar 13 10:36:42 2012 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Tue Mar 13 10:36:54 2012 Subject: [Histonet] Color blind chart request Message-ID: Google "Color blind chart". Then select "images" from the google menu. You will get what you want. Select the image and print on a color printer. Viola! Done. Stephen G. Ruby -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 13, 2012 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 100, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: undecalcified bone IHC (gayle callis) 2. Re: Re: undecalcified bone IHC (Victoria Baker) 3. Unsubscribe (Konop, Nicole) 4. Gram Stain (Giroux, Stacy) 5. DAKO Autostainer Issues (Friedrich_Hahn@bd.com) 6. Color Blindness Testing (Laurie@blufrogpath.com) 7. Re: DAKO Autostainer Issues (Drew Meyer) 8. IHC for decalcified bone protocol (Jeffery Howery) 9. Re: IHC for decalcified bone protocol (Mehmet Fatih BOZKURT) 10. RE: Gram Stain (gayle callis) 11. IHC Slides (Courtney Pierce) 12. Re: IHC Slides (Rene J Buesa) 13. RE: Gram Stain (Tony Henwood (SCHN)) 14. CD34 (Chakib Boussahmain) 15. (no subject) (enrriq88@yahoo.com) 16. Liver Biopsy Processing Schedule (ADESUPO ADESUYI) 17. CD45.1 & CD45.2 IF on parrafin sections. (Thotakura, Anil Kumar) 18. IHC Processor Validation & MUM1 (Bliven, Laura) 19. Ihc validation (Sabrina Townsend) 20. RE: Re: undecalcified bone IHC (Jack Ratliff) 21. Re: CD34 (Rene J Buesa) 22. Re: IHC Processor Validation & MUM1 (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Mar 2012 11:04:20 -0600 From: "gayle callis" Subject: [Histonet] Re: undecalcified bone IHC To: "'Histonet'" Message-ID: <000001cd0072$2cbb8150$863283f0$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Jeff, It is most certainly possible to do IHC on undecalcifed bone sections embedded in PMMA although not the easiest task. Sectioning is done on a microtome that is powerful enough to cut the plastic and using tungsten carbide knives. The key is total removal of the plastic from MMA embedded bone sections to allow antibody/ immunoglobulins to access antigenic sites. Neil Hand has done IHC successfully on PMMA embedded tissues including undecalcified bone on 2 to 3 ?m thick sections. I think one could cut thicker sections at 4 to 5 ?m and still be successful. I do not recall what Troiano et al used. The following publications will help you and should include protocols, although conventional protocols will work according to Hand. Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl methacrylate resin for embedding bone marrow trephine biopsies. Hand NM et al 1996 Antigen unmasking using microwave heating on formalin fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 Jackson P et al. 1996 Amplification of immunocytochemical reactions by the catalytic deposition of biotin on tissue sections. J Path 170(suppl):23A. This was about tyramide amplification when one gets a weak signal from "conventional" methods. Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and application in unmasking antigens embedded in methyl methacrylate. J Histotechnology 2`:231-236 Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light microscopy: a novel post embedding procedure. Proceeding Royal Microscopical Society 24(1):A54-55. Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory and Practice of Histological Technique, 5th edition by Gamble and Bancroft. The 6th edition is updated under same title. Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA embedded bone sections with publications in J Histotechnology. Hand mentioned several HIER methods, using citrate buffer. Optimizing retrieval will depend on the antigen and you may end up doing this with some form of HIER, including microwave or other heat producing methods and with different buffers. Enzyme digestion is also a possibility. Hand removed MMA with xylene, warm my speed up the removal, also more than one change for 10 - 20 minutes or longer. When I talked to him personally, he said he had used warm xylene although temperature was not mentioned in his chapter. After MMA removal, rehydrate section through alcohol gradient as one does paraffin sections. He was emphatic about never allowing the sections dry out. Hopefully Jack Ratliff and Damien Laudier will provide more insight on this topic. Good luck Gayle M. Callis HTL/HT/MT(ASCP) ****************************************** Hi Jeff, If is it possible a few more specifics of how the tissue has been received, processed and evaluated would help. Undecalcified bone sectioning procedures vary and also what specific markers are you looking to do is important. Vikki On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> wrote: > Undecalcified? How are you going to section it? > If you can section it, just use any IHC protocol for regular sections. > Good luck! > Ren? J. > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > From: Jeffery Howery jcl.com> > Subject: [Histonet] Undecalcified bone IHC > To: histonet <@t> lists.utsouthwestern.edu > Date: Monday, March 12, 2012, 10:59 AM > > > Does anyone have a protocol for Undecalcified bone for IHC? > ------------------------------ Message: 2 Date: Mon, 12 Mar 2012 13:09:39 -0400 From: Victoria Baker Subject: Re: [Histonet] Re: undecalcified bone IHC To: gayle callis Cc: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank you Gayle. Vikki On Mar 12, 2012 1:04 PM, "gayle callis" wrote: > Jeff, > > > > It is most certainly possible to do IHC on undecalcifed bone sections > embedded in PMMA although not the easiest task. Sectioning is done on a > microtome that is powerful enough to cut the plastic and using tungsten > carbide knives. The key is total removal of the plastic from MMA embedded > bone sections to allow antibody/ immunoglobulins to access antigenic sites. > Neil Hand has done IHC successfully on PMMA embedded tissues including > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > thicker sections at 4 to 5 ?m and still be successful. I do not > recall what Troiano et al used. > > > > The following publications will help you and should include protocols, > although conventional protocols will work according to Hand. > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > methacrylate resin for embedding bone marrow trephine biopsies. > > Hand NM et al 1996 Antigen unmasking using microwave heating on > formalin fixed tissue embedded in methyl methacrylate J Cellular Path > 1:31-37 > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > the catalytic deposition of biotin on tissue sections. J Path > 170(suppl):23A. This was about tyramide amplification when one gets a > weak signal from "conventional" methods. > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use > and application in unmasking antigens embedded in methyl methacrylate. > J Histotechnology 2`:231-236 > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for > light > microscopy: a novel post embedding procedure. Proceeding Royal > Microscopical Society 24(1):A54-55. > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory > and Practice of Histological Technique, 5th edition by Gamble and > Bancroft. > The 6th edition is updated under same title. > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on > PMMA embedded bone sections with publications in J Histotechnology. > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > retrieval will depend on the antigen and you may end up doing this > with some form of HIER, including microwave or other heat producing > methods and with > different buffers. Enzyme digestion is also a possibility. > > > > Hand removed MMA with xylene, warm my speed up the removal, also more than > one change for 10 - 20 minutes or longer. When I talked to him > personally, > he said he had used warm xylene although temperature was not mentioned in > his chapter. After MMA removal, rehydrate section through alcohol > gradient > as one does paraffin sections. He was emphatic about never allowing the > sections dry out. > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on > this topic. > > > > Good luck > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > ****************************************** > > > > Hi Jeff, > > > > If is it possible a few more specifics of how the tissue has been > received, > > processed and evaluated would help. Undecalcified bone sectioning > > procedures vary and also what specific markers are you looking to do > is > > important. > > > > Vikki > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa > yahoo.com> > wrote: > > > > > Undecalcified? How are you going to section it? > > > If you can section it, just use any IHC protocol for regular sections. > > > Good luck! > > > Ren? J. > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > > > > > > > From: Jeffery Howery jcl.com> > > > Subject: [Histonet] Undecalcified bone IHC > > > To: histonet <@t> lists.utsouthwestern.edu > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Mon, 12 Mar 2012 12:35:07 -0500 From: "Konop, Nicole" Subject: [Histonet] Unsubscribe To: "'histonet-request@lists.utsouthwestern.edu'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ------------------------------ Message: 4 Date: Mon, 12 Mar 2012 12:35:47 -0500 From: "Giroux, Stacy" Subject: [Histonet] Gram Stain To: "histonet@lists.utsouthwestern.edu" Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> Content-Type: text/plain; charset="iso-8859-1" Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ------------------------------ Message: 5 Date: Mon, 12 Mar 2012 14:31:44 -0400 From: Friedrich_Hahn@bd.com Subject: [Histonet] DAKO Autostainer Issues To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have been having issues with our Autostainer since bringing it out of storage. All parts are in excellent shape, according to the technician that performed the PM. It worked fine for a month of so, but then began malfunctioning after only a couple of staining runs. At some point in the staining process, the machine appears to lose its XY homing and jams itself against the front right corner, dispensing buffer onto the floor of the chamber and occasionally making grinding noises. We've had a technician take a look at it, 'clean' the software, and we thought we had it resolved, but the problem appears to be back. Has anyone experienced this issue? If so, was it hardware- or software- based? Thanks in advance! ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* ------------------------------ Message: 6 Date: Mon, 12 Mar 2012 11:34:20 -0700 From: Subject: [Histonet] Color Blindness Testing To: histonet@lists.utsouthwestern.edu Message-ID: <20120312113420.295dc6182df7e5cbb4f32bc101c30dcc.f82e5fb846.wbe@email15.secureserver.net> Content-Type: text/plain; charset="utf-8" Does anyone know of any (free) online testing for color blindness Does anyone have an alternate method that they have used to satisfy CAP requirements? Laurie ------------------------------ Message: 7 Date: Mon, 12 Mar 2012 14:42:44 -0400 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] DAKO Autostainer Issues To: "Friedrich_Hahn@bd.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <5FAEEDF0-63D7-4EBC-9A3E-AEF9D5297130@gmail.com> Content-Type: text/plain; charset=us-ascii There's a circuit board on the arm near the barcode scanner that controls the movement and orientation of the arm. If that circuitboard malfunctions, then you can see some of the erratic behavior that you are describing. That's one possibility I can think of that might cause that kind of problem. However, the technician who looked at it should be able to troubleshoot and replace that board if it is a problem. Drew Sent from my iPhone On Mar 12, 2012, at 2:31 PM, Friedrich_Hahn@bd.com wrote: > > We have been having issues with our Autostainer since bringing it out > of storage. All parts are in excellent shape, according to the > technician that performed the PM. It worked fine for a month of so, > but then began malfunctioning after only a couple of staining runs. > At some point in the staining process, the machine appears to lose its > XY homing and jams itself against the front right corner, dispensing > buffer onto the floor of the chamber and occasionally making grinding > noises. We've had a technician take a look at it, 'clean' the > software, and we thought we had it resolved, but the problem appears > to be back. > Has anyone experienced this issue? If so, was it hardware- or > software- based? > Thanks in advance! > > ----------------------------------------- > ******************************************************************* > IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may > constitute an advertisement of a BD group's products or services or a > solicitation of interest in them. If this is such a message and you > would like to opt out of receiving future advertisements or > solicitations from this BD group, please forward this e-mail to > optoutbygroup@bd.com. > ******************************************************************* > This message (which includes any attachments) is intended only for the > designated recipient(s). It may contain confidential or proprietary > information and may be subject to the attorney-client privilege or > other confidentiality protections. If you are not a designated > recipient, you may not review, use, copy or distribute this message. > If you received this in error, please notify the sender by reply > e-mail and delete this message. Thank you. > ******************************************************************* > Corporate Headquarters Mailing Address: BD (Becton, Dickinson and > Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. > ******************************************************************* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 12 Mar 2012 11:49:10 -0700 From: "Jeffery Howery" Subject: [Histonet] IHC for decalcified bone protocol To: Message-ID: Content-Type: text/plain; charset="us-ascii" Anyone have a Protocol for Decalcified bone for IHC? ------------------------------ Message: 9 Date: Mon, 12 Mar 2012 20:51:26 +0200 From: Mehmet Fatih BOZKURT Subject: Re: [Histonet] IHC for decalcified bone protocol To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 cool !! On Mon, Mar 12, 2012 at 8:49 PM, Jeffery Howery wrote: > Anyone have a Protocol for Decalcified bone for IHC? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ------------------------------ Message: 10 Date: Mon, 12 Mar 2012 13:08:55 -0600 From: "gayle callis" Subject: RE: [Histonet] Gram Stain To: "'Giroux, Stacy'" , Message-ID: <000001cd0083$93bf5c80$bb3e1580$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Stacy, You can buy a complete Brown and Brenn staining kit from Newcomer Supply which is very good, and not change your method. The picric acid/acetone mixture is included and you wouldn't have to store stock picric acid nor ether in the lab but just this small amount of Picric acid/acetone that comes in the kit. Disposal should not be that much of a problem. They also have a Hucker Twort Gram stain that uses acetone for destaining and you supply the acetone. Poly Scientific has Gram Stain kit (Hucker modification) which uses Grams decolorizer, a mixture of acetone and alcohol. They have excellent staining kits too. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Monday, March 12, 2012 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 12 Mar 2012 15:35:48 -0400 From: Courtney Pierce Subject: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Having problems with IHC slides not looking the same between runs. Does it have to do with how long and at what temp they are in the oven for? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ ------------------------------ Message: 12 Date: Mon, 12 Mar 2012 12:52:12 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" , Courtney Pierce Message-ID: <1331581932.14770.YahooMailClassic@web162101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Not really. Time in the oven and temp is usually not an issue as long as the tissue is well fixed/processed/infiltrated. Ren? J. --- On Mon, 3/12/12, Courtney Pierce wrote: From: Courtney Pierce Subject: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 12, 2012, 3:35 PM Having problems with IHC slides not looking the same between runs. Does it have to do with how long and at what temp they are in the oven for? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital **********************? IMPORTANT--PLEASE READ? ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information.? If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited.? If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 12 Mar 2012 23:38:11 +0000 From: "Tony Henwood (SCHN)" Subject: [Histonet] RE: Gram Stain To: "'Giroux, Stacy'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A715760A20038@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" Stacy, There are several Gram stain variants that do not use Picric acid. Tworts variant uses fast green and neutral red after the crystal violet-Iodine steps. Brown-Hopps method uses Basic Fuchsin, Gallego's differentiator and 1.5% Tartrazine after the crystal-violet steps, to stain the gram negative bugs. A simple red counterstain is to stain sections with 1% aqueous neutral red for 3 min after the crystal violet-Iodine steps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Tuesday, 13 March 2012 4:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 14 Date: Mon, 12 Mar 2012 18:19:14 -0700 (PDT) From: Chakib Boussahmain Subject: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu Message-ID: <1331601554.30627.YahooMailClassic@web161804.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello histonet, Does anyone using CD34? if so, can you share the staining protocol with us? and what are you using for antigen retrieval? Thank you so much. Chakib Boussahmain HTL(ASCP) ------------------------------ Message: 15 Date: Mon, 12 Mar 2012 18:25:44 -0700 (PDT) From: enrriq88@yahoo.com Subject: [Histonet] (no subject) To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1331601944.56861.YahooMailNeo@web113503.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Please?unsubscribe. thanks ------------------------------ Message: 16 Date: Mon, 12 Mar 2012 21:26:20 -0400 From: ADESUPO ADESUYI Subject: [Histonet] Liver Biopsy Processing Schedule To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I was wondering if anyone can share their LIVER Biopsy Processing Schedule with me? Thanks, Ade ------------------------------ Message: 17 Date: Tue, 13 Mar 2012 11:06:01 +0000 From: "Thotakura, Anil Kumar" Subject: [Histonet] CD45.1 & CD45.2 IF on parrafin sections. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear All, I want to do immunofluorescence on formalin fixed paraffin sections, Can you guys help me sending protocol how to do it ? Thank you very much for your help. BW Anil ------------------------------ Message: 18 Date: Tue, 13 Mar 2012 08:29:17 -0500 From: "Bliven, Laura" Subject: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" Message-ID: <201203131329.q2DDTXa8031218@mailhost3.mfldclin.edu> Content-Type: text/plain; charset="iso-8859-1" 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ Message: 19 Date: Tue, 13 Mar 2012 08:54:27 -0500 From: Sabrina Townsend Subject: [Histonet] Ihc validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <27E73F93-AB37-4260-8BEA-35F23AF4F022@gmail.com> Content-Type: text/plain; charset=us-ascii I am having to validate antibodies and was wondering if you have to do a parallel study or just use known positive and negative tissue? ~Sabrina~ ------------------------------ Message: 20 Date: Tue, 13 Mar 2012 10:50:23 -0400 From: Jack Ratliff Subject: RE: [Histonet] Re: undecalcified bone IHC To: Gayle Callis , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I might also add that Neil Hand is co-speaking with myself and Philip Seifert this year at the annual National Society for Histotechnology - Symposium/Convention in Vancouver B.C. Our workshop is titled: Resin Applications Forum: Methods for Processing, Special Staining, Immunohistochemical and In Situ Hybridization of Soft and Hard Tissue Including Medical Device Implants During the last 50 years, numerous histological procedures have been described on resin embedded tissue. While different types of resins are available for different purposes, the acrylics provide the widest range of techniques, especially for light microscopy applications. However, as demand from H&E to more sophisticated techniques increases, so too have the problems, and nowhere is this more apparent and controversial than in the application of immunohistochemistry on resin sections. This workshop will provide a review and discussion for those individuals that currently work with and/or are just getting started working with soft and hard tissue specimens and specifically the various resins (i.e. MMA, GMA, Technovit, Acrylosin, etc.) associated with their specific tissue interests. The workshop will also detail the preparation and staining of sections of soft and hard tissue, including implants (e.g. undemineralized bone and cardiovascular stents), for immunohistochemical and in situ hybridization staining using different acrylic and epoxy resin embedding media. Specific problems and pitfalls, either technical or operational associated with certain resin embedding procedures, will be illustrated and examined. Particular emphasis will be given to procedures which have been used extensively for routine diagnostic, and research purposes, i.e. those that WORK! Individuals with a current or future intent to process and cut undemineralized tissue or tissue containing foreign implant materials using acrylic or epoxy resins are strongly encouraged to attend this workshop! Please feel free to contact me if you would like more information about the workshop as information relevant to the exact date and time becomes available. All I know at this time is that the NSH meeting is September 29th - October 3rd, 2012. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology > From: gayle.callis@bresnan.net > To: histonet@lists.utsouthwestern.edu > Date: Mon, 12 Mar 2012 11:04:20 -0600 > Subject: [Histonet] Re: undecalcified bone IHC > > Jeff, > > > > It is most certainly possible to do IHC on undecalcifed bone sections > embedded in PMMA although not the easiest task. Sectioning is done on a > microtome that is powerful enough to cut the plastic and using tungsten > carbide knives. The key is total removal of the plastic from MMA embedded > bone sections to allow antibody/ immunoglobulins to access antigenic sites. > Neil Hand has done IHC successfully on PMMA embedded tissues including > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > thicker sections at 4 to 5 ?m and still be successful. I do not recall what > Troiano et al used. > > > > The following publications will help you and should include protocols, > although conventional protocols will work according to Hand. > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > methacrylate resin for embedding bone marrow trephine biopsies. > > Hand NM et al 1996 Antigen unmasking using microwave heating on formalin > fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > the catalytic deposition of biotin on tissue sections. J Path > 170(suppl):23A. This was about tyramide amplification when one gets a weak > signal from "conventional" methods. > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and > application in unmasking antigens embedded in methyl methacrylate. J > Histotechnology 2`:231-236 > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light > microscopy: a novel post embedding procedure. Proceeding Royal > Microscopical Society 24(1):A54-55. > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory > and Practice of Histological Technique, 5th edition by Gamble and Bancroft. > The 6th edition is updated under same title. > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA > embedded bone sections with publications in J Histotechnology. > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > retrieval will depend on the antigen and you may end up doing this with some > form of HIER, including microwave or other heat producing methods and with > different buffers. Enzyme digestion is also a possibility. > > > > Hand removed MMA with xylene, warm my speed up the removal, also more than > one change for 10 - 20 minutes or longer. When I talked to him personally, > he said he had used warm xylene although temperature was not mentioned in > his chapter. After MMA removal, rehydrate section through alcohol gradient > as one does paraffin sections. He was emphatic about never allowing the > sections dry out. > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on this > topic. > > > > Good luck > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > ****************************************** > > > > Hi Jeff, > > > > If is it possible a few more specifics of how the tissue has been received, > > processed and evaluated would help. Undecalcified bone sectioning > > procedures vary and also what specific markers are you looking to do is > > important. > > > > Vikki > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> > wrote: > > > > > Undecalcified? How are you going to section it? > > > If you can section it, just use any IHC protocol for regular sections. > > > Good luck! > > > Ren? J. > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > > > > > > > From: Jeffery Howery jcl.com> > > > Subject: [Histonet] Undecalcified bone IHC > > > To: histonet <@t> lists.utsouthwestern.edu > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 13 Mar 2012 08:06:35 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu, Chakib Boussahmain Message-ID: <1331651195.30522.YahooMailClassic@web162106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Becton-Dickinson Monoclonal antibody at 1:100 for 30 minutes after HIER (citrate at pH6) with placenta as control. Ren? J. --- On Mon, 3/12/12, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 9:19 PM Hello histonet, Does anyone using CD34? if so, can you share the staining protocol with us? and what are you using for antigen retrieval? Thank you so much. Chakib Boussahmain HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 13 Mar 2012 08:25:50 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" , LauraBliven Message-ID: <1331652350.10883.YahooMailClassic@web162101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 CAP has some guidelines as to the number of specimens and antibodies to test. I think to remember that the lowest amount was 25 specimens, but I do not remember about the antibodies but it makes sense to use only those your use to work with. Ren? J. --- On Tue, 3/13/12, Bliven, Laura wrote: From: Bliven, Laura Subject: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 13, 2012, 9:29 AM 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 15 ***************************************** From jqb7 <@t> cdc.gov Tue Mar 13 10:38:40 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 13 10:40:39 2012 Subject: [Histonet] RE: Color blind chart request In-Reply-To: References: Message-ID: You probably have to have someone administer it and verify it to make it acceptable for CAP, though. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen G. Ruby Sent: Tuesday, March 13, 2012 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Color blind chart request Google "Color blind chart". Then select "images" from the google menu. You will get what you want. Select the image and print on a color printer. Viola! Done. Stephen G. Ruby -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 13, 2012 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 100, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: undecalcified bone IHC (gayle callis) 2. Re: Re: undecalcified bone IHC (Victoria Baker) 3. Unsubscribe (Konop, Nicole) 4. Gram Stain (Giroux, Stacy) 5. DAKO Autostainer Issues (Friedrich_Hahn@bd.com) 6. Color Blindness Testing (Laurie@blufrogpath.com) 7. Re: DAKO Autostainer Issues (Drew Meyer) 8. IHC for decalcified bone protocol (Jeffery Howery) 9. Re: IHC for decalcified bone protocol (Mehmet Fatih BOZKURT) 10. RE: Gram Stain (gayle callis) 11. IHC Slides (Courtney Pierce) 12. Re: IHC Slides (Rene J Buesa) 13. RE: Gram Stain (Tony Henwood (SCHN)) 14. CD34 (Chakib Boussahmain) 15. (no subject) (enrriq88@yahoo.com) 16. Liver Biopsy Processing Schedule (ADESUPO ADESUYI) 17. CD45.1 & CD45.2 IF on parrafin sections. (Thotakura, Anil Kumar) 18. IHC Processor Validation & MUM1 (Bliven, Laura) 19. Ihc validation (Sabrina Townsend) 20. RE: Re: undecalcified bone IHC (Jack Ratliff) 21. Re: CD34 (Rene J Buesa) 22. Re: IHC Processor Validation & MUM1 (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Mar 2012 11:04:20 -0600 From: "gayle callis" Subject: [Histonet] Re: undecalcified bone IHC To: "'Histonet'" Message-ID: <000001cd0072$2cbb8150$863283f0$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Jeff, It is most certainly possible to do IHC on undecalcifed bone sections embedded in PMMA although not the easiest task. Sectioning is done on a microtome that is powerful enough to cut the plastic and using tungsten carbide knives. The key is total removal of the plastic from MMA embedded bone sections to allow antibody/ immunoglobulins to access antigenic sites. Neil Hand has done IHC successfully on PMMA embedded tissues including undecalcified bone on 2 to 3 ?m thick sections. I think one could cut thicker sections at 4 to 5 ?m and still be successful. I do not recall what Troiano et al used. The following publications will help you and should include protocols, although conventional protocols will work according to Hand. Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl methacrylate resin for embedding bone marrow trephine biopsies. Hand NM et al 1996 Antigen unmasking using microwave heating on formalin fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 Jackson P et al. 1996 Amplification of immunocytochemical reactions by the catalytic deposition of biotin on tissue sections. J Path 170(suppl):23A. This was about tyramide amplification when one gets a weak signal from "conventional" methods. Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and application in unmasking antigens embedded in methyl methacrylate. J Histotechnology 2`:231-236 Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for light microscopy: a novel post embedding procedure. Proceeding Royal Microscopical Society 24(1):A54-55. Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory and Practice of Histological Technique, 5th edition by Gamble and Bancroft. The 6th edition is updated under same title. Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA embedded bone sections with publications in J Histotechnology. Hand mentioned several HIER methods, using citrate buffer. Optimizing retrieval will depend on the antigen and you may end up doing this with some form of HIER, including microwave or other heat producing methods and with different buffers. Enzyme digestion is also a possibility. Hand removed MMA with xylene, warm my speed up the removal, also more than one change for 10 - 20 minutes or longer. When I talked to him personally, he said he had used warm xylene although temperature was not mentioned in his chapter. After MMA removal, rehydrate section through alcohol gradient as one does paraffin sections. He was emphatic about never allowing the sections dry out. Hopefully Jack Ratliff and Damien Laudier will provide more insight on this topic. Good luck Gayle M. Callis HTL/HT/MT(ASCP) ****************************************** Hi Jeff, If is it possible a few more specifics of how the tissue has been received, processed and evaluated would help. Undecalcified bone sectioning procedures vary and also what specific markers are you looking to do is important. Vikki On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> wrote: > Undecalcified? How are you going to section it? > If you can section it, just use any IHC protocol for regular sections. > Good luck! > Ren? J. > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > From: Jeffery Howery jcl.com> > Subject: [Histonet] Undecalcified bone IHC > To: histonet <@t> lists.utsouthwestern.edu > Date: Monday, March 12, 2012, 10:59 AM > > > Does anyone have a protocol for Undecalcified bone for IHC? > ------------------------------ Message: 2 Date: Mon, 12 Mar 2012 13:09:39 -0400 From: Victoria Baker Subject: Re: [Histonet] Re: undecalcified bone IHC To: gayle callis Cc: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thank you Gayle. Vikki On Mar 12, 2012 1:04 PM, "gayle callis" wrote: > Jeff, > > > > It is most certainly possible to do IHC on undecalcifed bone sections > embedded in PMMA although not the easiest task. Sectioning is done on a > microtome that is powerful enough to cut the plastic and using tungsten > carbide knives. The key is total removal of the plastic from MMA embedded > bone sections to allow antibody/ immunoglobulins to access antigenic sites. > Neil Hand has done IHC successfully on PMMA embedded tissues including > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > thicker sections at 4 to 5 ?m and still be successful. I do not > recall what Troiano et al used. > > > > The following publications will help you and should include protocols, > although conventional protocols will work according to Hand. > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > methacrylate resin for embedding bone marrow trephine biopsies. > > Hand NM et al 1996 Antigen unmasking using microwave heating on > formalin fixed tissue embedded in methyl methacrylate J Cellular Path > 1:31-37 > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > the catalytic deposition of biotin on tissue sections. J Path > 170(suppl):23A. This was about tyramide amplification when one gets a > weak signal from "conventional" methods. > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use > and application in unmasking antigens embedded in methyl methacrylate. > J Histotechnology 2`:231-236 > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for > light > microscopy: a novel post embedding procedure. Proceeding Royal > Microscopical Society 24(1):A54-55. > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory > and Practice of Histological Technique, 5th edition by Gamble and > Bancroft. > The 6th edition is updated under same title. > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on > PMMA embedded bone sections with publications in J Histotechnology. > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > retrieval will depend on the antigen and you may end up doing this > with some form of HIER, including microwave or other heat producing > methods and with > different buffers. Enzyme digestion is also a possibility. > > > > Hand removed MMA with xylene, warm my speed up the removal, also more than > one change for 10 - 20 minutes or longer. When I talked to him > personally, > he said he had used warm xylene although temperature was not mentioned in > his chapter. After MMA removal, rehydrate section through alcohol > gradient > as one does paraffin sections. He was emphatic about never allowing the > sections dry out. > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on > this topic. > > > > Good luck > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > ****************************************** > > > > Hi Jeff, > > > > If is it possible a few more specifics of how the tissue has been > received, > > processed and evaluated would help. Undecalcified bone sectioning > > procedures vary and also what specific markers are you looking to do > is > > important. > > > > Vikki > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa > yahoo.com> > wrote: > > > > > Undecalcified? How are you going to section it? > > > If you can section it, just use any IHC protocol for regular sections. > > > Good luck! > > > Ren? J. > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > > > > > > > From: Jeffery Howery jcl.com> > > > Subject: [Histonet] Undecalcified bone IHC > > > To: histonet <@t> lists.utsouthwestern.edu > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Mon, 12 Mar 2012 12:35:07 -0500 From: "Konop, Nicole" Subject: [Histonet] Unsubscribe To: "'histonet-request@lists.utsouthwestern.edu'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ------------------------------ Message: 4 Date: Mon, 12 Mar 2012 12:35:47 -0500 From: "Giroux, Stacy" Subject: [Histonet] Gram Stain To: "histonet@lists.utsouthwestern.edu" Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3E0709B0E@AUSP03VMBX11.apptixhealth.net> Content-Type: text/plain; charset="iso-8859-1" Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ------------------------------ Message: 5 Date: Mon, 12 Mar 2012 14:31:44 -0400 From: Friedrich_Hahn@bd.com Subject: [Histonet] DAKO Autostainer Issues To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have been having issues with our Autostainer since bringing it out of storage. All parts are in excellent shape, according to the technician that performed the PM. It worked fine for a month of so, but then began malfunctioning after only a couple of staining runs. At some point in the staining process, the machine appears to lose its XY homing and jams itself against the front right corner, dispensing buffer onto the floor of the chamber and occasionally making grinding noises. We've had a technician take a look at it, 'clean' the software, and we thought we had it resolved, but the problem appears to be back. Has anyone experienced this issue? If so, was it hardware- or software- based? Thanks in advance! ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* ------------------------------ Message: 6 Date: Mon, 12 Mar 2012 11:34:20 -0700 From: Subject: [Histonet] Color Blindness Testing To: histonet@lists.utsouthwestern.edu Message-ID: <20120312113420.295dc6182df7e5cbb4f32bc101c30dcc.f82e5fb846.wbe@email15.secureserver.net> Content-Type: text/plain; charset="utf-8" Does anyone know of any (free) online testing for color blindness Does anyone have an alternate method that they have used to satisfy CAP requirements? Laurie ------------------------------ Message: 7 Date: Mon, 12 Mar 2012 14:42:44 -0400 From: Drew Meyer <41dmb41@gmail.com> Subject: Re: [Histonet] DAKO Autostainer Issues To: "Friedrich_Hahn@bd.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <5FAEEDF0-63D7-4EBC-9A3E-AEF9D5297130@gmail.com> Content-Type: text/plain; charset=us-ascii There's a circuit board on the arm near the barcode scanner that controls the movement and orientation of the arm. If that circuitboard malfunctions, then you can see some of the erratic behavior that you are describing. That's one possibility I can think of that might cause that kind of problem. However, the technician who looked at it should be able to troubleshoot and replace that board if it is a problem. Drew Sent from my iPhone On Mar 12, 2012, at 2:31 PM, Friedrich_Hahn@bd.com wrote: > > We have been having issues with our Autostainer since bringing it out > of storage. All parts are in excellent shape, according to the > technician that performed the PM. It worked fine for a month of so, > but then began malfunctioning after only a couple of staining runs. > At some point in the staining process, the machine appears to lose its > XY homing and jams itself against the front right corner, dispensing > buffer onto the floor of the chamber and occasionally making grinding > noises. We've had a technician take a look at it, 'clean' the > software, and we thought we had it resolved, but the problem appears > to be back. > Has anyone experienced this issue? If so, was it hardware- or > software- based? > Thanks in advance! > > ----------------------------------------- > ******************************************************************* > IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may > constitute an advertisement of a BD group's products or services or a > solicitation of interest in them. If this is such a message and you > would like to opt out of receiving future advertisements or > solicitations from this BD group, please forward this e-mail to > optoutbygroup@bd.com. > ******************************************************************* > This message (which includes any attachments) is intended only for the > designated recipient(s). It may contain confidential or proprietary > information and may be subject to the attorney-client privilege or > other confidentiality protections. If you are not a designated > recipient, you may not review, use, copy or distribute this message. > If you received this in error, please notify the sender by reply > e-mail and delete this message. Thank you. > ******************************************************************* > Corporate Headquarters Mailing Address: BD (Becton, Dickinson and > Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. > ******************************************************************* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 12 Mar 2012 11:49:10 -0700 From: "Jeffery Howery" Subject: [Histonet] IHC for decalcified bone protocol To: Message-ID: Content-Type: text/plain; charset="us-ascii" Anyone have a Protocol for Decalcified bone for IHC? ------------------------------ Message: 9 Date: Mon, 12 Mar 2012 20:51:26 +0200 From: Mehmet Fatih BOZKURT Subject: Re: [Histonet] IHC for decalcified bone protocol To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 cool !! On Mon, Mar 12, 2012 at 8:49 PM, Jeffery Howery wrote: > Anyone have a Protocol for Decalcified bone for IHC? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ------------------------------ Message: 10 Date: Mon, 12 Mar 2012 13:08:55 -0600 From: "gayle callis" Subject: RE: [Histonet] Gram Stain To: "'Giroux, Stacy'" , Message-ID: <000001cd0083$93bf5c80$bb3e1580$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Stacy, You can buy a complete Brown and Brenn staining kit from Newcomer Supply which is very good, and not change your method. The picric acid/acetone mixture is included and you wouldn't have to store stock picric acid nor ether in the lab but just this small amount of Picric acid/acetone that comes in the kit. Disposal should not be that much of a problem. They also have a Hucker Twort Gram stain that uses acetone for destaining and you supply the acetone. Poly Scientific has Gram Stain kit (Hucker modification) which uses Grams decolorizer, a mixture of acetone and alcohol. They have excellent staining kits too. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Monday, March 12, 2012 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 12 Mar 2012 15:35:48 -0400 From: Courtney Pierce Subject: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Having problems with IHC slides not looking the same between runs. Does it have to do with how long and at what temp they are in the oven for? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ ------------------------------ Message: 12 Date: Mon, 12 Mar 2012 12:52:12 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" , Courtney Pierce Message-ID: <1331581932.14770.YahooMailClassic@web162101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Not really. Time in the oven and temp is usually not an issue as long as the tissue is well fixed/processed/infiltrated. Ren? J. --- On Mon, 3/12/12, Courtney Pierce wrote: From: Courtney Pierce Subject: [Histonet] IHC Slides To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 12, 2012, 3:35 PM Having problems with IHC slides not looking the same between runs. Does it have to do with how long and at what temp they are in the oven for? Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital **********************? IMPORTANT--PLEASE READ? ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information.? If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited.? If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 12 Mar 2012 23:38:11 +0000 From: "Tony Henwood (SCHN)" Subject: [Histonet] RE: Gram Stain To: "'Giroux, Stacy'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A715760A20038@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" Stacy, There are several Gram stain variants that do not use Picric acid. Tworts variant uses fast green and neutral red after the crystal violet-Iodine steps. Brown-Hopps method uses Basic Fuchsin, Gallego's differentiator and 1.5% Tartrazine after the crystal-violet steps, to stain the gram negative bugs. A simple red counterstain is to stain sections with 1% aqueous neutral red for 3 min after the crystal violet-Iodine steps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Tuesday, 13 March 2012 4:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 14 Date: Mon, 12 Mar 2012 18:19:14 -0700 (PDT) From: Chakib Boussahmain Subject: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu Message-ID: <1331601554.30627.YahooMailClassic@web161804.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello histonet, Does anyone using CD34? if so, can you share the staining protocol with us? and what are you using for antigen retrieval? Thank you so much. Chakib Boussahmain HTL(ASCP) ------------------------------ Message: 15 Date: Mon, 12 Mar 2012 18:25:44 -0700 (PDT) From: enrriq88@yahoo.com Subject: [Histonet] (no subject) To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1331601944.56861.YahooMailNeo@web113503.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Please?unsubscribe. thanks ------------------------------ Message: 16 Date: Mon, 12 Mar 2012 21:26:20 -0400 From: ADESUPO ADESUYI Subject: [Histonet] Liver Biopsy Processing Schedule To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I was wondering if anyone can share their LIVER Biopsy Processing Schedule with me? Thanks, Ade ------------------------------ Message: 17 Date: Tue, 13 Mar 2012 11:06:01 +0000 From: "Thotakura, Anil Kumar" Subject: [Histonet] CD45.1 & CD45.2 IF on parrafin sections. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear All, I want to do immunofluorescence on formalin fixed paraffin sections, Can you guys help me sending protocol how to do it ? Thank you very much for your help. BW Anil ------------------------------ Message: 18 Date: Tue, 13 Mar 2012 08:29:17 -0500 From: "Bliven, Laura" Subject: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" Message-ID: <201203131329.q2DDTXa8031218@mailhost3.mfldclin.edu> Content-Type: text/plain; charset="iso-8859-1" 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ Message: 19 Date: Tue, 13 Mar 2012 08:54:27 -0500 From: Sabrina Townsend Subject: [Histonet] Ihc validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <27E73F93-AB37-4260-8BEA-35F23AF4F022@gmail.com> Content-Type: text/plain; charset=us-ascii I am having to validate antibodies and was wondering if you have to do a parallel study or just use known positive and negative tissue? ~Sabrina~ ------------------------------ Message: 20 Date: Tue, 13 Mar 2012 10:50:23 -0400 From: Jack Ratliff Subject: RE: [Histonet] Re: undecalcified bone IHC To: Gayle Callis , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I might also add that Neil Hand is co-speaking with myself and Philip Seifert this year at the annual National Society for Histotechnology - Symposium/Convention in Vancouver B.C. Our workshop is titled: Resin Applications Forum: Methods for Processing, Special Staining, Immunohistochemical and In Situ Hybridization of Soft and Hard Tissue Including Medical Device Implants During the last 50 years, numerous histological procedures have been described on resin embedded tissue. While different types of resins are available for different purposes, the acrylics provide the widest range of techniques, especially for light microscopy applications. However, as demand from H&E to more sophisticated techniques increases, so too have the problems, and nowhere is this more apparent and controversial than in the application of immunohistochemistry on resin sections. This workshop will provide a review and discussion for those individuals that currently work with and/or are just getting started working with soft and hard tissue specimens and specifically the various resins (i.e. MMA, GMA, Technovit, Acrylosin, etc.) associated with their specific tissue interests. The workshop will also detail the preparation and staining of sections of soft and hard tissue, including implants (e.g. undemineralized bone and cardiovascular stents), for immunohistochemical and in situ hybridization staining using different acrylic and epoxy resin embedding media. Specific problems and pitfalls, either technical or operational associated with certain resin embedding procedures, will be illustrated and examined. Particular emphasis will be given to procedures which have been used extensively for routine diagnostic, and research purposes, i.e. those that WORK! Individuals with a current or future intent to process and cut undemineralized tissue or tissue containing foreign implant materials using acrylic or epoxy resins are strongly encouraged to attend this workshop! Please feel free to contact me if you would like more information about the workshop as information relevant to the exact date and time becomes available. All I know at this time is that the NSH meeting is September 29th - October 3rd, 2012. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology > From: gayle.callis@bresnan.net > To: histonet@lists.utsouthwestern.edu > Date: Mon, 12 Mar 2012 11:04:20 -0600 > Subject: [Histonet] Re: undecalcified bone IHC > > Jeff, > > > > It is most certainly possible to do IHC on undecalcifed bone sections > embedded in PMMA although not the easiest task. Sectioning is done on > a microtome that is powerful enough to cut the plastic and using > tungsten carbide knives. The key is total removal of the plastic from > MMA embedded bone sections to allow antibody/ immunoglobulins to access antigenic sites. > Neil Hand has done IHC successfully on PMMA embedded tissues including > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > thicker sections at 4 to 5 ?m and still be successful. I do not recall > what Troiano et al used. > > > > The following publications will help you and should include protocols, > although conventional protocols will work according to Hand. > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > methacrylate resin for embedding bone marrow trephine biopsies. > > Hand NM et al 1996 Antigen unmasking using microwave heating on > formalin fixed tissue embedded in methyl methacrylate J Cellular Path > 1:31-37 > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > the catalytic deposition of biotin on tissue sections. J Path > 170(suppl):23A. This was about tyramide amplification when one gets a > weak signal from "conventional" methods. > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use > and application in unmasking antigens embedded in methyl methacrylate. > J Histotechnology 2`:231-236 > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for > light > microscopy: a novel post embedding procedure. Proceeding Royal > Microscopical Society 24(1):A54-55. > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. > Theory and Practice of Histological Technique, 5th edition by Gamble and Bancroft. > The 6th edition is updated under same title. > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on > PMMA embedded bone sections with publications in J Histotechnology. > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > retrieval will depend on the antigen and you may end up doing this > with some form of HIER, including microwave or other heat producing > methods and with different buffers. Enzyme digestion is also a possibility. > > > > Hand removed MMA with xylene, warm my speed up the removal, also more > than one change for 10 - 20 minutes or longer. When I talked to him > personally, he said he had used warm xylene although temperature was > not mentioned in his chapter. After MMA removal, rehydrate section > through alcohol gradient as one does paraffin sections. He was > emphatic about never allowing the sections dry out. > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on > this topic. > > > > Good luck > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > ****************************************** > > > > Hi Jeff, > > > > If is it possible a few more specifics of how the tissue has been > received, > > processed and evaluated would help. Undecalcified bone sectioning > > procedures vary and also what specific markers are you looking to do > is > > important. > > > > Vikki > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa > yahoo.com> > wrote: > > > > > Undecalcified? How are you going to section it? > > > If you can section it, just use any IHC protocol for regular sections. > > > Good luck! > > > Ren? J. > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> wrote: > > > > > > > > > From: Jeffery Howery jcl.com> > > > Subject: [Histonet] Undecalcified bone IHC > > > To: histonet <@t> lists.utsouthwestern.edu > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 13 Mar 2012 08:06:35 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu, Chakib Boussahmain Message-ID: <1331651195.30522.YahooMailClassic@web162106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Becton-Dickinson Monoclonal antibody at 1:100 for 30 minutes after HIER (citrate at pH6) with placenta as control. Ren? J. --- On Mon, 3/12/12, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] CD34 To: histonet@lists.utsouthwestern.edu Date: Monday, March 12, 2012, 9:19 PM Hello histonet, Does anyone using CD34? if so, can you share the staining protocol with us? and what are you using for antigen retrieval? Thank you so much. Chakib Boussahmain HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 13 Mar 2012 08:25:50 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" , LauraBliven Message-ID: <1331652350.10883.YahooMailClassic@web162101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 CAP has some guidelines as to the number of specimens and antibodies to test. I think to remember that the lowest amount was 25 specimens, but I do not remember about the antibodies but it makes sense to use only those your use to work with. Ren? J. --- On Tue, 3/13/12, Bliven, Laura wrote: From: Bliven, Laura Subject: [Histonet] IHC Processor Validation & MUM1 To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 13, 2012, 9:29 AM 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 15 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Tue Mar 13 10:41:00 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Tue Mar 13 10:41:14 2012 Subject: [Histonet] RE: Histonet Digest, Vol 100, Issue 15 In-Reply-To: <20120313153114.F0F838B70CC@mx10.myoutlookonline.com> References: <20120313153114.F0F838B70CC@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB5C07E@S10MAILD001N1.SH10.lan> We also have a first generation Autostainer and I have had the same happen with my machine. I believe it is definitely a mechanical issue. The engineer that came out changed the motor driver unit and we haven't had a problem since. He also told me that it can also be the belt drive on the arm that drives the XY that could be a problem if it persists. Get another service call in and get them back out to fix the unit. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 5 Date: Mon, 12 Mar 2012 14:31:44 -0400 From: Friedrich_Hahn@bd.com Subject: [Histonet] DAKO Autostainer Issues To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have been having issues with our Autostainer since bringing it out of storage. All parts are in excellent shape, according to the technician that performed the PM. It worked fine for a month of so, but then began malfunctioning after only a couple of staining runs. At some point in the staining process, the machine appears to lose its XY homing and jams itself against the front right corner, dispensing buffer onto the floor of the chamber and occasionally making grinding noises. We've had a technician take a look at it, 'clean' the software, and we thought we had it resolved, but the problem appears to be back. Has anyone experienced this issue? If so, was it hardware- or software- based? Thanks in advance! ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* From Diane.Tokugawa <@t> kp.org Tue Mar 13 10:44:11 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Tue Mar 13 10:44:26 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 03/13/2012 and will not return until 03/19/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From rsrichmond <@t> gmail.com Tue Mar 13 10:45:17 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Mar 13 10:45:25 2012 Subject: [Histonet] Re: Gram stain Message-ID: Stacy Giroux (where?) asks: >>Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good?<< I'd want to ask your pathologists: do you really need this stain? If you want to see or count bacteria, a simple Giemsa type stain (such as Diff-Quik) will do the job. Tissue Gram stains are arduous to perform, and do not work well, particularly for staining Gram negatives. The Gram stain on smears is of course the workhorse of microbiology, but it does not work well on tissue sections. Bob Richmond Samurai Pathologist Knoxville TN From dmlaud <@t> gmail.com Tue Mar 13 10:55:44 2012 From: dmlaud <@t> gmail.com (Damien) Date: Tue Mar 13 10:55:50 2012 Subject: [Histonet] Re: undecalcified bone IHC In-Reply-To: References: <000001cd0072$2cbb8150$863283f0$@bresnan.net> Message-ID: Excellent! Happy to read the venerable Neil Hand is coming back to the symposium, always a great speaker! -Damien On Tue, Mar 13, 2012 at 10:50 AM, Jack Ratliff wrote: > > I might also add that Neil Hand is co-speaking with myself and Philip > Seifert this year at the annual National Society for Histotechnology - > Symposium/Convention in Vancouver B.C. Our workshop is titled: > > Resin Applications Forum: Methods for Processing, Special Staining, > Immunohistochemical and In Situ Hybridization of Soft and Hard Tissue > Including Medical Device Implants > > During the last 50 years, numerous histological procedures have been > described on resin embedded tissue. While different types of resins are > available for different purposes, the acrylics provide the widest range of > techniques, especially for light microscopy applications. However, as > demand from H&E to more sophisticated techniques increases, so too have the > problems, and nowhere is this more apparent and controversial than in the > application of immunohistochemistry on resin sections. This workshop will > provide a review and discussion for those individuals that currently work > with and/or are just getting started working with soft and hard tissue > specimens and specifically the various resins (i.e. MMA, GMA, Technovit, > Acrylosin, etc.) associated with their specific tissue interests. The > workshop will also detail the preparation and staining of sections of soft > and hard tissue, including implants (e.g. undemineralized bone and > cardiovascular stents), for immunohistochemical and in situ hybridization > staining using different acrylic and epoxy resin embedding media. Specific > problems and pitfalls, either technical or operational associated with > certain resin embedding procedures, will be illustrated and examined. > Particular emphasis will be given to procedures which have been used > extensively for routine diagnostic, and research purposes, i.e. those that > WORK! Individuals with a current or future intent to process and cut > undemineralized tissue or tissue containing foreign implant materials using > acrylic or epoxy resins are strongly encouraged to attend this workshop! > > Please feel free to contact me if you would like more information about > the workshop as information relevant to the exact date and time becomes > available. All I know at this time is that the NSH meeting is September > 29th - October 3rd, 2012. > > Best Regards, > > Jack > > > Jack Ratliff > Hard Tissue Histologist > Chairman, Hard Tissue Committee - National Society for Histotechnology > > > > > > From: gayle.callis@bresnan.net > > To: histonet@lists.utsouthwestern.edu > > Date: Mon, 12 Mar 2012 11:04:20 -0600 > > Subject: [Histonet] Re: undecalcified bone IHC > > > > Jeff, > > > > > > > > It is most certainly possible to do IHC on undecalcifed bone sections > > embedded in PMMA although not the easiest task. Sectioning is done on a > > microtome that is powerful enough to cut the plastic and using tungsten > > carbide knives. The key is total removal of the plastic from MMA embedded > > bone sections to allow antibody/ immunoglobulins to access antigenic > sites. > > Neil Hand has done IHC successfully on PMMA embedded tissues including > > undecalcified bone on 2 to 3 ?m thick sections. I think one could cut > > thicker sections at 4 to 5 ?m and still be successful. I do not recall > what > > Troiano et al used. > > > > > > > > The following publications will help you and should include protocols, > > although conventional protocols will work according to Hand. > > > > > > > > Blythe D. Hand N et al 1997 J Clin Path 50:45-49. The use of methyl > > methacrylate resin for embedding bone marrow trephine biopsies. > > > > Hand NM et al 1996 Antigen unmasking using microwave heating on formalin > > fixed tissue embedded in methyl methacrylate J Cellular Path 1:31-37 > > > > Jackson P et al. 1996 Amplification of immunocytochemical reactions by > > the catalytic deposition of biotin on tissue sections. J Path > > 170(suppl):23A. This was about tyramide amplification when one gets a > weak > > signal from "conventional" methods. > > > > Hand NM, Church RJ 1998 Superheating using pressure cooking: its use and > > application in unmasking antigens embedded in methyl methacrylate. J > > Histotechnology 2`:231-236 > > > > Hand NM et al 1989 Immunohistochemistry on resin embedded tissue for > light > > microscopy: a novel post embedding procedure. Proceeding Royal > > Microscopical Society 24(1):A54-55. > > > > Hand NM Plastic Embedding media and techniques, Ch.30, p 663-677. Theory > > and Practice of Histological Technique, 5th edition by Gamble and > Bancroft. > > The 6th edition is updated under same title. > > > > > > > > Use Google Scholar to find Troiano N et al from Yale on doing IHC on PMMA > > embedded bone sections with publications in J Histotechnology. > > > > > > > > > > > > Hand mentioned several HIER methods, using citrate buffer. Optimizing > > retrieval will depend on the antigen and you may end up doing this with > some > > form of HIER, including microwave or other heat producing methods and > with > > different buffers. Enzyme digestion is also a possibility. > > > > > > > > Hand removed MMA with xylene, warm my speed up the removal, also more > than > > one change for 10 - 20 minutes or longer. When I talked to him > personally, > > he said he had used warm xylene although temperature was not mentioned in > > his chapter. After MMA removal, rehydrate section through alcohol > gradient > > as one does paraffin sections. He was emphatic about never allowing the > > sections dry out. > > > > > > > > Hopefully Jack Ratliff and Damien Laudier will provide more insight on > this > > topic. > > > > > > > > Good luck > > > > > > > > Gayle M. Callis > > > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > > > > > > > > > > > > > > > ****************************************** > > > > > > > > Hi Jeff, > > > > > > > > If is it possible a few more specifics of how the tissue has been > received, > > > > processed and evaluated would help. Undecalcified bone sectioning > > > > procedures vary and also what specific markers are you looking to do is > > > > important. > > > > > > > > Vikki > > > > On Mon, Mar 12, 2012 at 11:06 AM, Rene J Buesa yahoo.com> > > wrote: > > > > > > > > > Undecalcified? How are you going to section it? > > > > > If you can section it, just use any IHC protocol for regular sections. > > > > > Good luck! > > > > > Ren? J. > > > > > > > > > > --- On Mon, 3/12/12, Jeffery Howery jcl.com> > wrote: > > > > > > > > > > > > > > > From: Jeffery Howery jcl.com> > > > > > Subject: [Histonet] Undecalcified bone IHC > > > > > To: histonet <@t> lists.utsouthwestern.edu > > > > > Date: Monday, March 12, 2012, 10:59 AM > > > > > > > > > > > > > > > Does anyone have a protocol for Undecalcified bone for IHC? > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From rsrichmond <@t> gmail.com Tue Mar 13 10:58:59 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Mar 13 10:59:06 2012 Subject: [Histonet] Re: Color Blindness Testing Message-ID: Laurie asks: >>Does anyone know of any (free) online testing for color blindness? Does anyone have an alternate method that they have used to satisfy CAP requirements?<< Here are some online Ishihara plates quickly found through Wikipedia: http://offsetpressman.blogspot.com/2009/10/color-blindness-test-for-printers.html There are many others. Two reservations: Color monitors don't reproduce colors with sufficient accuracy. And an online test may not satisfy bureaucrats. You'd have to ask. I have normal color vision, but I've been interested in color blindness among pathologists for about 50 years. I know of only one major problem, finding red acid-fast bacilli in a blue-background stain. This problem should be solved by banning light-microscopic acid-fast stains, something the CAP should have done years ago. I don't like the implication of color-blindness testing in the absence of a significant problem. Can somebody lose their job, or not get hired in the first place, because of color-blindness? It may be Good Management, but it's not good medicine or good social policy. Bob Richmond Samurai Pathologist Knoxville TN From mdpraet <@t> gmail.com Tue Mar 13 11:24:41 2012 From: mdpraet <@t> gmail.com (mequita praet) Date: Tue Mar 13 11:24:49 2012 Subject: [Histonet] Re: Brown and Brenn Message-ID: Stacy, I use a procedure that was published in the Journal of Histotechnology, Vol 25, No2, June 2002. *Modifying the Modification: How to Redden Shy Gram Negative.* It has worked for me consistently for many years without the picric acid. It does use some acetone. I get the ragents from PolyScientific. If you don't have copies of the Journal, just email me at mdpraet@gmail.com and I will send it to you. Mequita Praet From patjnm <@t> gwumc.edu Tue Mar 13 11:28:05 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Tue Mar 13 11:28:17 2012 Subject: [Histonet] Brown and Brenn Message-ID: <4F5F3D55.DB55.001F.1@gwumc.edu> You can switch to Tartrazine. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From TMcNemar <@t> lmhealth.org Tue Mar 13 12:21:56 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Mar 13 12:22:08 2012 Subject: [Histonet] Re: Color Blindness Testing In-Reply-To: References: Message-ID: I've been watching this thread with great interest.... I have been in Histology for 30+ years now and am myself colorblind (at least according to the Unites States Navy). I do see color even though I could not pass the old card test (failed it 3 times). My problem is with shades of color. I cannot distinguish real light shades or real dark shades. I started my laboratory life about 32 years ago on an aircraft carrier and was trained to read diffs, ph strips, etc. by a senior lab tech who was... you guessed it, color blind! The point of all of this is that you can do histology even if you cannot pass the test for colorblindness. For myself, if I know what a color is supposed to be and what that color looks like to me, I generally do not have any problems at all. As mentioned below, I too have trouble with acid fast stains. I cannot judge the stain quality of the acid fast stain and don't even bother to look at them. I was very nearly fired after only a short time in my present position when the Medical Director found out that I was colorblind. He couldn't see how I could possibly do histology. I convinced him to give me a chance and 28 years later I am still here. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, March 13, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Color Blindness Testing Laurie asks: >>Does anyone know of any (free) online testing for color blindness? Does anyone have an alternate method that they have used to satisfy CAP requirements?<< Here are some online Ishihara plates quickly found through Wikipedia: http://offsetpressman.blogspot.com/2009/10/color-blindness-test-for-printers.html There are many others. Two reservations: Color monitors don't reproduce colors with sufficient accuracy. And an online test may not satisfy bureaucrats. You'd have to ask. I have normal color vision, but I've been interested in color blindness among pathologists for about 50 years. I know of only one major problem, finding red acid-fast bacilli in a blue-background stain. This problem should be solved by banning light-microscopic acid-fast stains, something the CAP should have done years ago. I don't like the implication of color-blindness testing in the absence of a significant problem. Can somebody lose their job, or not get hired in the first place, because of color-blindness? It may be Good Management, but it's not good medicine or good social policy. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From NMP <@t> stowers.org Tue Mar 13 12:46:58 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Tue Mar 13 12:47:03 2012 Subject: [Histonet] Re: Color Blindness Testing In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF997F371E9@EXCHMB-02.stowers-institute.org> What a great story :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, March 13, 2012 12:22 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Color Blindness Testing I've been watching this thread with great interest.... I have been in Histology for 30+ years now and am myself colorblind (at least according to the Unites States Navy). I do see color even though I could not pass the old card test (failed it 3 times). My problem is with shades of color. I cannot distinguish real light shades or real dark shades. I started my laboratory life about 32 years ago on an aircraft carrier and was trained to read diffs, ph strips, etc. by a senior lab tech who was... you guessed it, color blind! The point of all of this is that you can do histology even if you cannot pass the test for colorblindness. For myself, if I know what a color is supposed to be and what that color looks like to me, I generally do not have any problems at all. As mentioned below, I too have trouble with acid fast stains. I cannot judge the stain quality of the acid fast stain and don't even bother to look at them. I was very nearly fired after only a short time in my present position when the Medical Director found out that I was colorblind. He couldn't see how I could possibly do histology. I convinced him to give me a chance and 28 years later I am still here. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, March 13, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Color Blindness Testing Laurie asks: >>Does anyone know of any (free) online testing for color blindness? Does anyone have an alternate method that they have used to satisfy CAP requirements?<< Here are some online Ishihara plates quickly found through Wikipedia: http://offsetpressman.blogspot.com/2009/10/color-blindness-test-for-printers.html There are many others. Two reservations: Color monitors don't reproduce colors with sufficient accuracy. And an online test may not satisfy bureaucrats. You'd have to ask. I have normal color vision, but I've been interested in color blindness among pathologists for about 50 years. I know of only one major problem, finding red acid-fast bacilli in a blue-background stain. This problem should be solved by banning light-microscopic acid-fast stains, something the CAP should have done years ago. I don't like the implication of color-blindness testing in the absence of a significant problem. Can somebody lose their job, or not get hired in the first place, because of color-blindness? It may be Good Management, but it's not good medicine or good social policy. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paw555 <@t> yahoo.com Tue Mar 13 13:20:32 2012 From: paw555 <@t> yahoo.com (pam plumlee) Date: Tue Mar 13 13:20:42 2012 Subject: [Histonet] H.T. Job Opp in San Diego Message-ID: <1331662832.39573.YahooMailNeo@web31905.mail.mud.yahoo.com> Possible full-time position?at a?cancer diagnostic CLIA/CAP lab in San Diego: Must be H.T.(ASCP) Must have excellent cutting skills Must have IHC experience (we use the BondMax) LMD experience a plus or desire to learn (a must!) prefer local candidate.... ? email resume to: ? paw555@yahooo.com From akoug <@t> hotmail.com Tue Mar 13 13:24:48 2012 From: akoug <@t> hotmail.com (=?iso-8859-1?B?QW5kcukgSw==?=) Date: Tue Mar 13 13:24:54 2012 Subject: [Histonet] End-point of decalcification vs. Ion exchange resins Message-ID: Hi, I've read at a couple of places that you can't use the chemical test to determine the end-point of decalcification if you used an ion-exchange resin for the decalcification. I understand that calcium ions are captured by the resin, therefore they can't be precipitated by the ammonium oxalate. My question is : Since you need to refresh the decalcifying solution prior to the test, would it be possible to perform the test by switching to a resin-free solution just before performing the test? Thanks for your answer Andre From cjbulmer <@t> sbcglobal.net Tue Mar 13 13:29:37 2012 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Tue Mar 13 13:29:41 2012 Subject: [Histonet] IHC Stainers Message-ID: <1331663377.79054.YahooMailClassic@web82307.mail.mud.yahoo.com> Hello Techs, ? I would like to get some feedback on the Dako Autostainer Link 48, using the Flex reagents. ? Thank you, Cindy? Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From rjbuesa <@t> yahoo.com Tue Mar 13 14:14:35 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 13 14:14:40 2012 Subject: [Histonet] End-point of decalcification vs. Ion exchange resins In-Reply-To: Message-ID: <1331666075.81441.YahooMailClassic@web162102.mail.bf1.yahoo.com> If you do that all the advantages of using the ion-exchange resin will be lost. Simply determine the end point of the decalcification by other usual and more "traditional" methods, like specimen bending or a pressure/penetration test. You could also use X-ray with a Faxitron. Ren? J. --- On Tue, 3/13/12, Andr? K wrote: From: Andr? K Subject: [Histonet] End-point of decalcification vs. Ion exchange resins To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 13, 2012, 2:24 PM Hi, I've read at a couple of places that you can't use the chemical test to determine the end-point of decalcification if you used an ion-exchange resin for the decalcification. I understand that calcium ions are captured by the resin, therefore they can't be precipitated by the ammonium oxalate. My question is : Since you need to refresh the decalcifying solution prior to the test, would it be possible to perform the test by switching to a resin-free solution just before performing the test? Thanks for your answer Andre ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.DeMint <@t> phci.org Tue Mar 13 14:22:10 2012 From: Valerie.DeMint <@t> phci.org (DeMint, Valerie S) Date: Tue Mar 13 14:22:12 2012 Subject: [Histonet] Thermo Richard Allen Screen Cassettes Message-ID: <657B95ED46A98B4D9F44EFC67EDCAADD04AFC7A4@RWDEX3.WHS.phci.org> Looking for help again....Has anyone been experiencing problems with the ink printing coming off the Thermo Histoscreens after processing? This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From ASelf <@t> georgetownhospitalsystem.org Tue Mar 13 14:43:03 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Mar 13 14:43:16 2012 Subject: [Histonet] CPT Code Question Message-ID: Happy almost Hump Day Everyone, Does anyone use CPT code 88399 or 89240 for any type of unlisted or miscellaneous pathology test or procedures? If so what type of test/procedures are you using the code for? Also is there a CPT code that can be used for gathering up pathology material for consultation when another facility is requesting the use of your blocks/slides for continued patient care? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From tuyenmai77 <@t> yahoo.com Tue Mar 13 15:15:14 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Tue Mar 13 15:15:22 2012 Subject: [Histonet] Greetings, friend! Message-ID: <1331669714.75725.yint-ygo-j2me@web162801.mail.bf1.yahoo.com> Greetings, friend! http://metalgeek.zxq.net/response.php?urjluqjbahuma=79&gjcewip=977&ygoxab=38 From billodonnell <@t> catholichealth.net Tue Mar 13 15:18:16 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Mar 13 15:18:56 2012 Subject: [Histonet] Re: Color Blindness Testing In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395479DC5@chimsx08.CHI.catholichealth.net> Almost exact same situation with me Tom. Navy refused my application for Nuclear Engineering and made me a Medical Technologist instead. It struck me as funny the first time I had to identify an eosiniphil. Then I went to histolgy. Apparently the Navy didn't want me around things that could endanger a lot of people at once...... Just one at a time. William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, March 13, 2012 12:22 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Color Blindness Testing I've been watching this thread with great interest.... I have been in Histology for 30+ years now and am myself colorblind (at least according to the Unites States Navy). I do see color even though I could not pass the old card test (failed it 3 times). My problem is with shades of color. I cannot distinguish real light shades or real dark shades. I started my laboratory life about 32 years ago on an aircraft carrier and was trained to read diffs, ph strips, etc. by a senior lab tech who was... you guessed it, color blind! The point of all of this is that you can do histology even if you cannot pass the test for colorblindness. For myself, if I know what a color is supposed to be and what that color looks like to me, I generally do not have any problems at all. As mentioned below, I too have trouble with acid fast stains. I cannot judge the stain quality of the acid fast stain and don't even bother to look at them. I was very nearly fired after only a short time in my present position when the Medical Director found out that I was colorblind. He couldn't see how I could possibly do histology. I convinced him to give me a chance and 28 years later I am still here. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, March 13, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Color Blindness Testing Laurie asks: >>Does anyone know of any (free) online testing for color blindness? Does anyone have an alternate method that they have used to satisfy CAP requirements?<< Here are some online Ishihara plates quickly found through Wikipedia: http://offsetpressman.blogspot.com/2009/10/color-blindness-test-for-prin ters.html There are many others. Two reservations: Color monitors don't reproduce colors with sufficient accuracy. And an online test may not satisfy bureaucrats. You'd have to ask. I have normal color vision, but I've been interested in color blindness among pathologists for about 50 years. I know of only one major problem, finding red acid-fast bacilli in a blue-background stain. This problem should be solved by banning light-microscopic acid-fast stains, something the CAP should have done years ago. I don't like the implication of color-blindness testing in the absence of a significant problem. Can somebody lose their job, or not get hired in the first place, because of color-blindness? It may be Good Management, but it's not good medicine or good social policy. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From one_angel_secret <@t> yahoo.com Tue Mar 13 15:34:33 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 13 15:34:45 2012 Subject: [Histonet] CPT Code Question In-Reply-To: References: Message-ID: A long time ago I tried to use 88399 misc for prep work my techs were doing for various test we sent out. We never got reimbursed for anything on that code. Maybe others here had better luck than me with it. You seem to be thinking a long the line I was about all the work we do that we don't get to charge for. Now why I have never tried to charge for recuts for consults we alwYs charged a flat fee when it was legal cases to go to attorneys offices. KD Sent from my iPhone On Mar 13, 2012, at 3:43 PM, Amy Self wrote: > Happy almost Hump Day Everyone, > > Does anyone use CPT code 88399 or 89240 for any type of unlisted or miscellaneous pathology test or procedures? If so what type of test/procedures are you using the code for? > > Also is there a CPT code that can be used for gathering up pathology material for consultation when another facility is requesting the use of your blocks/slides for continued patient care? > > > Thanks in advance for your help, > Amy > > > Amy Self > Georgetown Hospital System > 843-527-7179 > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Mar 13 15:43:14 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 13 15:43:34 2012 Subject: [Histonet] IHC Stainers In-Reply-To: <1331663377.79054.YahooMailClassic@web82307.mail.mud.yahoo.com> References: <1331663377.79054.YahooMailClassic@web82307.mail.mud.yahoo.com> Message-ID: <055495FC-212D-477D-BB27-4AD7299EE84B@yahoo.com> I love it. Great turn around time. Great crisp clean stains. And I think it's easy to use. Some will say ah it has the off line Retrieval. But I like the fact that the samples are submerged into a solution. Call me old fashioned? I've used pretty much all of the platforms. They all have good stuff and not so good. I'd recommend the Dako link. Kim Donadio Sent from my iPhone On Mar 13, 2012, at 2:29 PM, Cindy Bulmer wrote: > Hello Techs, > > I would like to get some feedback on the Dako Autostainer Link 48, using the > Flex reagents. > > Thank you, > Cindy > > Cynthia Bulmer HT(ASCP)QIHC > IHC Supervisor, CTPL > Waco, TX > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Mar 13 15:49:13 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 13 15:50:33 2012 Subject: [Histonet] IHC Processor Validation & MUM1 In-Reply-To: <201203131329.q2DDTXa8031218@mailhost3.mfldclin.edu> References: <201203131329.q2DDTXa8031218@mailhost3.mfldclin.edu> Message-ID: CAP/ASCO recommends at least 25 cases for ER, PR and Her2. I just did this a few months ago. As far as the rest verify that each of the ab you are doing work and compare them to what you were doing before. How many it takes to sastify your pathologist that the others are working and they sign off on them should be ok. Hope this helps. Kim D Sent from my iPhone On Mar 13, 2012, at 9:29 AM, "Bliven, Laura" wrote: > > 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one. > > 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone. > > Thanks, > Laura > > ______________________________________________________________________ > The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- End -- > From azdudley <@t> hotmail.com Tue Mar 13 16:49:11 2012 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Mar 13 16:49:14 2012 Subject: [Histonet] ventana uv alkaline phosphatase red Message-ID: for those of you that use the ultraview alk phos red on their machines, do you run the slides through alcohols? we are having a lot of trouble with the staining, sometimes it is good others no staining at all on our mart 1s or pin4 prostate bxs. I was told that we should not put them in alc. air dry after water or oven dry? just wondering is that how others are handleing their slides stained with the red kit? we have been running them down through the alc. with the dab ones. thanks so much anita dudley providence hosp mobile alabama From histotalk <@t> yahoo.com Tue Mar 13 20:17:11 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Mar 13 20:17:16 2012 Subject: [Histonet] Did I mention... Message-ID: <1331687831.73261.YahooMailNeo@web120602.mail.ne1.yahoo.com> Hi everyone - Did I mention that Damien Laudier from Laudier Histology was a guest last Sunday, March 11th on HistoTALK? Or that Vinnie Della Speranza from MUSC Health will be our guest on Sunday, March 25th? No? Well, Damien Laudier from Laudier Histology was a guest last Sunday, March 11th and Vinnie Della Speranza will be our guest on Sunday, March 25th. Why not listen in at www.HistoTALK.com? ? Yours, David ? Oh, yes...the Region III meeting will be hosted by the GSH on April 13-15 at Calloway Gardens, Pine Mountain, GA -?HistoTALK will be there doing interviews for future shows. Thanks Wanda and the GSH Board for the invitation! From tony.henwood <@t> health.nsw.gov.au Tue Mar 13 22:34:05 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Mar 13 22:34:17 2012 Subject: [Histonet] Re: Gram stain In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A2061D@xmdb02.nch.kids> I agree with Bob wholeheartedly as well as agree 100% and I am unanimous in that! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, 14 March 2012 2:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gram stain Stacy Giroux (where?) asks: >>Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good?<< I'd want to ask your pathologists: do you really need this stain? If you want to see or count bacteria, a simple Giemsa type stain (such as Diff-Quik) will do the job. Tissue Gram stains are arduous to perform, and do not work well, particularly for staining Gram negatives. The Gram stain on smears is of course the workhorse of microbiology, but it does not work well on tissue sections. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Melissa.Kuhnla <@t> chsli.org Wed Mar 14 04:18:10 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Mar 14 04:19:10 2012 Subject: [Histonet] ventana uv alkaline phosphatase red In-Reply-To: References: Message-ID: Hi, When Ventana reformulated their RED detection kit a while back, they adviced to remove alcohol from the Coverslipping process. As per their instructions, we do the following: Remove slides from staining platform Rinse with Dawn and warm water Dry slides in 60 degree oven for 15 minutes A few quick dips in fresh Xylene Coverslip -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, March 13, 2012 5:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventana uv alkaline phosphatase red for those of you that use the ultraview alk phos red on their machines, do you run the slides through alcohols? we are having a lot of trouble with the staining, sometimes it is good others no staining at all on our mart 1s or pin4 prostate bxs. I was told that we should not put them in alc. air dry after water or oven dry? just wondering is that how others are handleing their slides stained with the red kit? we have been running them down through the alc. with the dab ones. thanks so much anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Lynn.Burton <@t> Illinois.gov Wed Mar 14 08:14:33 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Mar 14 08:17:11 2012 Subject: [Histonet] ventana uv alkaline phosphatase red In-Reply-To: References: Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00649C40B05@IL084EXMBX214.illinois.gov> The ultra view platform changed its clearing process in November. After staining slides are to be run through two changes of soapy water and then distilled water. After that is done they go on a slide dryer/warmer for at least 15 minutes before going into xylene and then coverslipped. Our sales rep made me aware of the change when it happened. I opened a new kit yesterday and what I just wrote is what the package insert said to do. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdudley@hotmail.com] Sent: Tuesday, March 13, 2012 4:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventana uv alkaline phosphatase red for those of you that use the ultraview alk phos red on their machines, do you run the slides through alcohols? we are having a lot of trouble with the staining, sometimes it is good others no staining at all on our mart 1s or pin4 prostate bxs. I was told that we should not put them in alc. air dry after water or oven dry? just wondering is that how others are handleing their slides stained with the red kit? we have been running them down through the alc. with the dab ones. thanks so much anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Mar 14 08:52:32 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Mar 14 08:52:40 2012 Subject: [Histonet] Reference lab doing GLG-1 (MG-160)? Message-ID: Does anyone know of a reference lab offering this antibody for aggressive pituitary tumors in FFPE human tissue? Thanks, Linda From dmccaig <@t> ckha.on.ca Wed Mar 14 09:23:27 2012 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Mar 14 09:27:44 2012 Subject: [Histonet] restaining negative stain with synaptophysin Message-ID: We have no tissue in a block and would like to take the negative control and stain it for synaptophysin. We are using the Nemesis, Biocare Mach 4, AP protocol. Sincere thanks Diana McCaig From ebreisch <@t> rchsd.org Wed Mar 14 10:57:49 2012 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Wed Mar 14 10:57:55 2012 Subject: [Histonet] SV-40 control tissue Message-ID: <243B05306324D84E8153380CDABC4F76010DAA1BC517@EXBE1.RCHSD.org> Does anyone have a commercial source for SV-40 control tissue to be used in IMPX? Thanks for your help. EAB Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital San Diego Associate Clinical Professor of Anatomy Dept. of Surgery/Div. of Anatomy UCSD School of Medicine LaJolla, CA From gliuygao <@t> hotmail.com Wed Mar 14 12:11:03 2012 From: gliuygao <@t> hotmail.com (yan gao) Date: Wed Mar 14 11:11:13 2012 Subject: [Histonet] incredible Message-ID: Click here to see the attached video From gliuygao <@t> hotmail.com Wed Mar 14 12:11:34 2012 From: gliuygao <@t> hotmail.com (yan gao) Date: Wed Mar 14 11:11:41 2012 Subject: [Histonet] incredible Message-ID: Click here to see the attached video From Marilyn.A.Weiss <@t> kp.org Wed Mar 14 12:00:18 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Mar 14 12:00:36 2012 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 03/14/2012 and will not return until 03/15/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From Ken_Marissael <@t> vwr.com Wed Mar 14 12:02:53 2012 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Wed Mar 14 12:03:05 2012 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 03/14/2012 and will not return until 03/15/2012. I will be away from March 14th for training. I will have limited access to cell and e-mail. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. From karengary <@t> mplpath.com Wed Mar 14 12:10:55 2012 From: karengary <@t> mplpath.com (Karen Gary) Date: Wed Mar 14 12:10:55 2012 Subject: [Histonet] Histotech Position Message-ID: <001201cd0205$6b176600$41463200$@mplpath.com> Seeking a qualified histology tech for full time position. Private pathology lab located in East Texas. Monday - Friday 6:30 - 3:00. Please send resume to karengary@mplpath.com for more detailed information. Karen S. Gary HT (ASCP), BBA Laboratory Manager Medical & Pathology Lab 402 N. 5th Street Longview, TX 75601 903.758.8511 ext. 201 903.758.8528 Confidentiality Notice: The information contained in this transmission may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately notify me by telephone and permanently delete the original and any copy of this e-mail and destroy any printout thereof. From TJohnson <@t> gnf.org Wed Mar 14 12:11:08 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Wed Mar 14 12:12:54 2012 Subject: [Histonet] Diluted secondary antibody stability Message-ID: <9F3CFEE76E51B64991C7485270890B4009F0ECBD@EX5.lj.gnf.org> Hi all, What is your general rule of thumb for the stability of diluted secondary antibodies? What is the longest you will store them for reuse? I know for many, you make them up fresh when you need them, especially for small volume users. But those of us who use automation, what is your lab practice? Thanks in advance, Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From ncosenza <@t> siumed.edu Wed Mar 14 13:39:42 2012 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Wed Mar 14 13:39:46 2012 Subject: [Histonet] re-usability of ethanols Message-ID: <4F60E5EE.3070105@siumed.edu> Hello all, I am looking for opinions on how long/often I can re-use ethanols (100%, 95%, 50%) when I rehydrate paraffin sections for IHC staining. Thanks! From Luis.Chiriboga <@t> nyumc.org Wed Mar 14 14:51:56 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Wed Mar 14 14:52:03 2012 Subject: [Histonet] HIF1a Message-ID: <3E6798F00C9F494399E96B720ECD1429EC8F@MSGWCDCPMB22.nyumc.org> Can anyone recommend ab for HIF1a with reactivity in both human and mouse ? Human over mouse preferred. Thanks Luis Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYUSOM Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 Luis.Chiriboga@nyumc.org From rjbuesa <@t> yahoo.com Wed Mar 14 14:56:06 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 14 14:56:15 2012 Subject: [Histonet] re-usability of ethanols In-Reply-To: <4F60E5EE.3070105@siumed.edu> Message-ID: <1331754966.48926.YahooMailClassic@web162101.mail.bf1.yahoo.com> Do not reuse once you have changed them. Ren? J. --- On Wed, 3/14/12, Nicole Cosenza wrote: From: Nicole Cosenza Subject: [Histonet] re-usability of ethanols To: Histonet@lists.utsouthwestern.edu Date: Wednesday, March 14, 2012, 2:39 PM Hello all, I am looking for opinions on how long/often I can re-use ethanols (100%, 95%, 50%) when I rehydrate paraffin sections for IHC staining. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slakmon <@t> yahoo.com Wed Mar 14 21:42:20 2012 From: slakmon <@t> yahoo.com (Philip Slakmon) Date: Wed Mar 14 21:42:28 2012 Subject: [Histonet] Diamond Knives Message-ID: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... Thank you, Philip From JMacDonald <@t> mtsac.edu Thu Mar 15 00:47:17 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Mar 15 00:48:24 2012 Subject: [Histonet] PAS Diastase Message-ID: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer From tuyenmai77 <@t> yahoo.com Thu Mar 15 03:36:58 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Thu Mar 15 03:37:07 2012 Subject: [Histonet] Fw: Message-ID: <1331800618.4616.yint-ygo-j2me@web162804.mail.bf1.yahoo.com> Greetings, friend! http://morcego.ind.br/response.php?ezoponiperufiv=97&edawiho=258&afuhaf=52 From patpxs <@t> gmail.com Thu Mar 15 07:28:39 2012 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Mar 15 07:28:43 2012 Subject: [Histonet] Diamond Knives In-Reply-To: References: Message-ID: Hi Philip, Diatome is the brand we use. Lately they have been having resharpening issues. We have had to send several back after resharpening because the knives left scratch marks on the thin sections. That said, they are friendly and helpful and have never complained about us returning one for additional resharpening. There are other brands on the market, I have not used them in a while so I cannot comment about those vendors. If you have anyother questions, feel free to ask. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Wed, Mar 14, 2012 at 10:42 PM, Philip Slakmon wrote: > Good Afternoon, > > I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... > > > Thank you, > > Philip > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 15 08:07:53 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 15 08:08:00 2012 Subject: [Histonet] PAS Diastase In-Reply-To: Message-ID: <1331816873.65182.YahooMailClassic@web162105.mail.bf1.yahoo.com> No, you are correct. The tech should always give the pathologists one with and another without diastase. Ren? J. --- On Thu, 3/15/12, Jennifer MacDonald wrote: From: Jennifer MacDonald Subject: [Histonet] PAS Diastase To: histonet@lists.utsouthwestern.edu Date: Thursday, March 15, 2012, 1:47 AM Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase.? They do not do an undigested slide.? How would the pathologist know if the digested slide had a glycogen to begin with?? Am I over thinking this? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Thu Mar 15 08:19:45 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 15 08:19:57 2012 Subject: [Histonet] Diamond Knives In-Reply-To: References: Message-ID: Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon wrote: > Good Afternoon, > > I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... > > > Thank you, > > Philip > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b-frederick <@t> northwestern.edu Thu Mar 15 08:23:53 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 15 08:24:02 2012 Subject: [Histonet] Diamond Knives In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E1B8F20@evcspmbx3.ads.northwestern.edu> We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 8:20 AM To: Philip Slakmon Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon wrote: > Good Afternoon, > > I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... > > > Thank you, > > Philip > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Mar 15 08:54:41 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Mar 15 08:53:34 2012 Subject: [Histonet] PAS Diastase In-Reply-To: References: Message-ID: <4F61F4A1.8030105@umdnj.edu> I agree with Rene. A control (undigested) slide is necessary. Geoff On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested slide > had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From boneimage8 <@t> gmail.com Thu Mar 15 09:10:25 2012 From: boneimage8 <@t> gmail.com (Marc DeCarlo) Date: Thu Mar 15 09:10:33 2012 Subject: [Histonet] Diamond Knives In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E1B8F20@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E1B8F20@evcspmbx3.ads.northwestern.edu> Message-ID: Philip, We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. Marc On Thursday, March 15, 2012, Bernice Frederick wrote: > We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff > Sent: Thursday, March 15, 2012 8:20 AM > To: Philip Slakmon > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Diamond Knives > > Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. > > Best Regards, > > Jack > > > > On Mar 14, 2012, at 9:42 PM, Philip Slakmon wrote: > >> Good Afternoon, >> >> I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... >> >> >> Thank you, >> >> Philip >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From karabou76 <@t> hotmail.com Thu Mar 15 09:10:34 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Thu Mar 15 09:10:42 2012 Subject: [Histonet] Vacuum processing question Message-ID: Hello all, I have a question about vacuum processing. We have ligament tissues that are processed over night, then are to be vacuum processed. I've never done this before, but was able to get ahold of a vacuum oven. >From what I understand, I just put the tissues in a metal container and turn on the vacuum oven for 1 hour. But what are they supposed to be in? Parafin? Alcohol? Do I just leave them in the cassettes and not put them in any sort of liquid? Is there a specific temperature or vacuum that any of you have used that works particularly well? Also, is it possible to let the tissues sit over the weekend after the regular processing before putting them in the vacuum oven for processing? Any advice would be greatly appreciated as I am new to this world of histology, having a biochemistry background. Many thanks in advance, Kara From bakerj <@t> umich.edu Thu Mar 15 09:13:28 2012 From: bakerj <@t> umich.edu (John Baker) Date: Thu Mar 15 09:13:42 2012 Subject: [Histonet] Processor Preferences?? Message-ID: Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From rjbuesa <@t> yahoo.com Thu Mar 15 09:23:44 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 15 09:23:52 2012 Subject: [Histonet] Processor Preferences?? In-Reply-To: Message-ID: <1331821424.86511.YahooMailClassic@web162101.mail.bf1.yahoo.com> Of the 3 you mention, I would buy VIP6. Let's see what Jennifer answers. Ren? J. --- On Thu, 3/15/12, John Baker wrote: From: John Baker Subject: [Histonet] Processor Preferences?? To: histonet@lists.utsouthwestern.edu Date: Thursday, March 15, 2012, 10:13 AM Hello Jennifer,? I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase?? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6.? Your thoughts on your choice and on these listed.? Thanks you,? John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Mar 15 09:31:47 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 15 09:31:51 2012 Subject: [Histonet] Processor Preferences?? In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E1B8F62@evcspmbx3.ads.northwestern.edu> If it was me and it is in the future, I want a Leica Peloris. Leica also has a new one that is a single chamber version of the Peloris. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Baker Sent: Thursday, March 15, 2012 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 15 10:10:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 15 10:10:58 2012 Subject: [Histonet] Vacuum processing question In-Reply-To: Message-ID: <1331824250.38067.YahooMailClassic@web162106.mail.bf1.yahoo.com> Vacuum processing has been advocated in the past as a way to process, but the penetration of liquid is not a function of negative pressure ("vacuum") but of positive pressure. In the initial experiments with the Peloris instrument in Australia (by BioSystems before it was bought by Leica Microsystems) they subjected the tissues that were dehydrated with ethanol to high temperature under vacuum because they needed to eliminate the ethanol before the hot paraffin wax infiltration because ethanol absolutely does not mix with paraffin. Once they changed to isopropyl alcohol (that mixes with melted paraffin) the vacuum and heat step was reduced in time and intensity. The VIP uses vacuum in their retort as the only way the have to empty the retort but that vacuum step has effect on the tissues inside only during the few minutes the retort is empty, that?is when the tissues as subjected to vacuum. At that moment the liquids inside the tissues will exit them and "facilitate" the penetration of the next liquid that will enter the retort under pressure. Vacuum per se has no place in tissue processing except to facilitate the elimination of some liquid that has already penetrated the tissue and as an intermediate step between other steps involving a liquid. I just do not?know why?you?expect to obtain with?your vacuum oven. They are usually used when you want to evaporate some liquid at a temperature below the boiling point. In the same way that a pressure cooker allows you to heat water at above 100?C without boiling, a vacuum oven will allow you to boil water at less than 100?C. Both variations of the boiling point (above or below) are an inverse relation with the (+) or (-) pressure. If you leave your specimens without any liquid in your vacuum oven, you will just dehydrate them. It seems that you have been asked to do this without any further instructions. Ask whomever requested you to do this what for you are supposed to do this. Never fear to ask anybody who instructs you to do something: why! Ren? J. --- On Thu, 3/15/12, Kara Lee wrote: From: Kara Lee Subject: [Histonet] Vacuum processing question To: histonet@lists.utsouthwestern.edu Date: Thursday, March 15, 2012, 10:10 AM Hello all, I have a question about vacuum processing. We have ligament tissues that are processed over night, then are to be vacuum processed.? I've never done this before, but was able to get ahold of a vacuum oven.? >From what I understand, I just put the tissues in a metal container and turn on the vacuum oven for 1 hour.? But what are they supposed to be in?? Parafin?? Alcohol?? Do I just leave them in the cassettes and not put them in any sort of liquid? Is there a specific temperature or vacuum that any of you have used that works particularly well? Also, is it possible to let the tissues sit over the weekend after the regular processing before putting them in the vacuum oven for processing? Any advice would be greatly appreciated as I am new to this world of histology, having a biochemistry background. Many thanks in advance, Kara ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kstoll <@t> mcw.edu Thu Mar 15 10:30:50 2012 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Thu Mar 15 10:30:58 2012 Subject: [Histonet] CDC42 Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34ED1ACBB1FE@MCWMBX2.mcwcorp.net> Does anyone have a good protocol for CDC42? The ab I am using if from santa cruz sc-8401. I stain on the dako autostainer plus using flex reagents. Kathryn Stoll, HT(ASCP) Department of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu From ratliffjack <@t> hotmail.com Thu Mar 15 10:54:50 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 15 10:54:59 2012 Subject: [Histonet] Processor Preferences?? In-Reply-To: References: Message-ID: John, Go for the ASP300S and then let's talk again at your convenience about resin infiltration needs. I hope the information I sent to you was both informative and helpful! Regards, Jack On Mar 15, 2012, at 9:13 AM, John Baker wrote: > Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John > > John A. Baker > The University of Michigan > Orthopaedic Research Laboratories > Histology Unit > 109 Zina Pitcher Place, 2218 BSRB > Ann Arbor, MI 48109-2200 > 734-936-1635 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Thu Mar 15 10:59:10 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 15 10:59:22 2012 Subject: [Histonet] Diamond Knives In-Reply-To: References: <62C639732D3F274DACED033EBDF6ADAF1E1B8F20@evcspmbx3.ads.northwestern.edu> Message-ID: Philip, Marc is correct in everything that he has stated, but if you are looking for diamond knife purchases or services as you indicated in your original posting, I am only aware of DDK providing this service. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 15, 2012, at 9:10 AM, Marc DeCarlo wrote: > Philip, > > We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. > > Marc > > On Thursday, March 15, 2012, Bernice Frederick wrote: > > We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff > > Sent: Thursday, March 15, 2012 8:20 AM > > To: Philip Slakmon > > Cc: Histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Diamond Knives > > > > Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. > > > > Best Regards, > > > > Jack > > > > > > > > On Mar 14, 2012, at 9:42 PM, Philip Slakmon wrote: > > > >> Good Afternoon, > >> > >> I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... > >> > >> > >> Thank you, > >> > >> Philip > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TJohnson <@t> gnf.org Thu Mar 15 11:21:47 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Thu Mar 15 11:21:57 2012 Subject: [Histonet] Re: PAS Diastase Message-ID: <9F3CFEE76E51B64991C7485270890B4009F0EED4@EX5.lj.gnf.org> I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested slide > had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From ree3 <@t> leicester.ac.uk Thu Mar 15 11:46:04 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Mar 15 11:46:12 2012 Subject: [Histonet] ACETONE FIXED TISSUES, GMA PROCESSED+IMMUNOFLUORESCENCE Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F926C2D8@EXC-MBX3.cfs.le.ac.uk> Anyone out there who has had success with the above, I would like to hear from you, many thanks. Richard Edwards University of Leicester U.K. From JMitchell <@t> uwhealth.org Thu Mar 15 12:07:07 2012 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Thu Mar 15 12:07:15 2012 Subject: [Histonet] Diamond Knives In-Reply-To: References: <62C639732D3F274DACED033EBDF6ADAF1E1B8F20@evcspmbx3.ads.northwestern.edu> Message-ID: Micro Star Technologies, Huntsville, Texas is also a supplier of diamond knives and a resharpening service. I have used them many times over the past 20+ years and have been satisfied with their product. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 10:59 AM To: Marc DeCarlo Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Philip, Marc is correct in everything that he has stated, but if you are looking for diamond knife purchases or services as you indicated in your original posting, I am only aware of DDK providing this service. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 15, 2012, at 9:10 AM, Marc DeCarlo wrote: > Philip, > > We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. > > Marc > > On Thursday, March 15, 2012, Bernice Frederick wrote: > > We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack > > Ratliff > > Sent: Thursday, March 15, 2012 8:20 AM > > To: Philip Slakmon > > Cc: Histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Diamond Knives > > > > Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. > > > > Best Regards, > > > > Jack > > > > > > > > On Mar 14, 2012, at 9:42 PM, Philip Slakmon wrote: > > > >> Good Afternoon, > >> > >> I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... > >> > >> > >> Thank you, > >> > >> Philip > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Thu Mar 15 12:32:33 2012 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Mar 15 12:32:57 2012 Subject: Subject: [Histonet] PAS Diastase Message-ID: <4F61D351020000A8000787EC@ns.luhcares.org> Jennifer, No you are not. Matt Lunetta BS HT(ASCP) Longmont United Hospital Message: 9 Date: Wed, 14 Mar 2012 22:47:17 -0700 From: Jennifer MacDonald Subject: [Histonet] PAS Diastase To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer From gu.lang <@t> gmx.at Thu Mar 15 13:09:29 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 15 13:09:40 2012 Subject: AW: [Histonet] Re: PAS Diastase In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F0EED4@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F0EED4@EX5.lj.gnf.org> Message-ID: It depends on the tissue-component to be shown. If you just want to see tissue-architecture or eg. in liver mallory-bodies and glycogen doesn't play a role, one slide would be sufficient. Gudrun On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested slide > had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duraine <@t> bcm.edu Thu Mar 15 13:25:44 2012 From: duraine <@t> bcm.edu (Duraine, Lita) Date: Thu Mar 15 13:25:52 2012 Subject: [Histonet] Diamond Knives In-Reply-To: References: <62C639732D3F274DACED033EBDF6ADAF1E1B8F20@evcspmbx3.ads.northwestern.edu> Message-ID: <9D2C4E815F57784DAE6914BA54C82324CAEE25AF24@EXCMSMBX06.ad.bcm.edu> Philip, Try also Diatome Diamond Knives. http://www.diatomeknives.com/ Regards, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Thursday, March 15, 2012 12:07 PM To: Jack Ratliff Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Diamond Knives Micro Star Technologies, Huntsville, Texas is also a supplier of diamond knives and a resharpening service. I have used them many times over the past 20+ years and have been satisfied with their product. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 10:59 AM To: Marc DeCarlo Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Philip, Marc is correct in everything that he has stated, but if you are looking for diamond knife purchases or services as you indicated in your original posting, I am only aware of DDK providing this service. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 15, 2012, at 9:10 AM, Marc DeCarlo wrote: > Philip, > > We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. > > Marc > > On Thursday, March 15, 2012, Bernice Frederick wrote: > > We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack > > Ratliff > > Sent: Thursday, March 15, 2012 8:20 AM > > To: Philip Slakmon > > Cc: Histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Diamond Knives > > > > Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. > > > > Best Regards, > > > > Jack > > > > > > > > On Mar 14, 2012, at 9:42 PM, Philip Slakmon wrote: > > > >> Good Afternoon, > >> > >> I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, .... > >> > >> > >> Thank you, > >> > >> Philip > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ArtimK <@t> slhn.org Thu Mar 15 15:29:58 2012 From: ArtimK <@t> slhn.org (Artim, Kimberly) Date: Thu Mar 15 15:30:08 2012 Subject: [Histonet] CD230 Message-ID: <9E67FDD215B226448638018A82B952BD026BCDCBF6AB@EXCHANGE.slhn.org> Does anyone have any experience with or knowledge of this antibody (CD230)? I would appreciate any comments/literature you could share. Thank you, Kim Kimberly R. Artim AST, HT (ASCP) St Luke's Specialty Lab 77 S. Commerce Way Bethlehem, PA 18017 Phone: 484-526-4832 artimk@slhn.org ********************************************************************** Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jackgyger <@t> yahoo.com Thu Mar 15 15:37:05 2012 From: jackgyger <@t> yahoo.com (Jack Gyger) Date: Thu Mar 15 15:37:09 2012 Subject: [Histonet] PIN-4 Message-ID: <1331843825.61728.YahooMailClassic@web160201.mail.bf1.yahoo.com> Does anyone have a good protocol for pin-4 on a Ventana XT or Ultra? From Laurie <@t> blufrogpath.com Thu Mar 15 16:15:43 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Thu Mar 15 16:15:52 2012 Subject: [Histonet] Validation of new equipment Message-ID: <20120315141543.295dc6182df7e5cbb4f32bc101c30dcc.11e0648fb5.wbe@email15.secureserver.net> If I am validating a new tissue processor, can I just process a v ariety of tissues and have the pathologist sign off that they are acce established pro Laurie From tony.henwood <@t> health.nsw.gov.au Thu Mar 15 17:26:37 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 15 17:26:54 2012 Subject: [Histonet] PAS Diastase In-Reply-To: <4F61F4A1.8030105@umdnj.edu> References: <4F61F4A1.8030105@umdnj.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A21DB4@xmdb02.nch.kids> My two cents: I agree as well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, 16 March 2012 12:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Diastase I agree with Rene. A control (undigested) slide is necessary. Geoff On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested > slide had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Mar 15 17:28:14 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 15 17:28:23 2012 Subject: Recall: [Histonet] PAS Diastase Message-ID: <6D6BD1DE8A5571489398B392A38A715760A21DBC@xmdb02.nch.kids> Tony Henwood (SCHN) would like to recall the message, "[Histonet] PAS Diastase". ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Mar 15 17:36:37 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 15 17:36:48 2012 Subject: [Histonet] PAS Diastase In-Reply-To: <4F61F4A1.8030105@umdnj.edu> References: <4F61F4A1.8030105@umdnj.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A21DB4@xmdb02.nch.kids> My two cents: I agree as well Though in some instances the presence of glycogen is neither here nor there. It might be removed just to make the diagnostic feature being sought easier to detect. For example, we used to do routinely do AB-DiPAS on oesophageal biopsies for the detection of epithelial metaplasia as well as fungus. In this case the presence of glycogen was thought to confuse things so was removed prior to PAS staining. The presence or absence of glycogen in nearly all cases was not diagnostic. In this case an AB-PAS was not done. Remember to look for internal controls for PAS staining (eg basement membranes). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, 16 March 2012 12:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Diastase I agree with Rene. A control (undigested) slide is necessary. Geoff On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested > slide had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Mar 15 17:39:06 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 15 17:39:23 2012 Subject: [Histonet] RE: Re: PAS Diastase In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009F0EED4@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009F0EED4@EX5.lj.gnf.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A21E51@xmdb02.nch.kids> No, If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well. (see my earlier Post) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, 16 March 2012 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: PAS Diastase I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested > slide had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jenniferscholler <@t> gmail.com Thu Mar 15 19:41:17 2012 From: jenniferscholler <@t> gmail.com (Jennifer Scholler) Date: Thu Mar 15 19:41:20 2012 Subject: [Histonet] Staining GAGs in fetal membranes Message-ID: Hello, I am attempting to stain fetal membranes to look at the GAG content. My advisor suggested Safronin O, which I tried on a preliminary set of slides (hematoxylin, fast green, then safronin O). I did get some staining and saw a band of red staining between the layers, as expected, but the staining is not nearly as pronounced as in images of Safronin O stained tissues that I have seen (some of this obviously may be due to imaging settings). Also, after doing some reading, it seems that Alcian blue may be another option. Can anyone with experience staining FMs or similar tissues suggest a stain and protocol for this? Thanks! Regards, Jennifer Scholler M.S. candidate Mechanical Engineering University of Colorado at Boulder From TJohnson <@t> gnf.org Thu Mar 15 19:43:37 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Thu Mar 15 19:43:42 2012 Subject: [Histonet] RE: Re: PAS Diastase In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A21E51@xmdb02.nch.kids> References: <9F3CFEE76E51B64991C7485270890B4009F0EED4@EX5.lj.gnf.org> <6D6BD1DE8A5571489398B392A38A715760A21E51@xmdb02.nch.kids> Message-ID: <9F3CFEE76E51B64991C7485270890B4009F0F2C4@EX5.lj.gnf.org> Tony, I agree, when looking for glycogen two patient slides should be run, one with, one without. That is not always the case. We were sometimes asked to do PAS/D on lymphomas. Teri -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Thursday, March 15, 2012 3:39 PM To: Theresa (Teri) Johnson; histonet@lists.utsouthwestern.edu Subject: RE: Re: PAS Diastase No, If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well. (see my earlier Post) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, 16 March 2012 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: PAS Diastase I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested > slide had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Mar 15 20:14:11 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 15 20:14:20 2012 Subject: [Histonet] Wet Workshops NSW Australia Message-ID: <6D6BD1DE8A5571489398B392A38A715760A21F1E@xmdb02.nch.kids> Hi all, We are looking at organising wet workshops in Sydney later this year (Wet workshop = hands on lab work). We ran a Histochem workshop last year at our National Meeting (twice) and many seemed happy with it. We are after themes for further workshops eg: Mucin Histochemistry H&E Staining Manual Immunohistochemistry Any other ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) and Linda Prasad MSc, BSc Histopathology Department Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From chak_bou <@t> yahoo.com Fri Mar 16 08:14:06 2012 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Fri Mar 16 08:14:12 2012 Subject: [Histonet] Calponin Message-ID: <1331903646.10510.YahooMailClassic@web161806.mail.bf1.yahoo.com> Hello Histonet Does anyone use Calponin stain? if so can you share the protocol with us? What do you use for epitope retrieval? Many thanks in advance. Chakib Boussahmain Histotechnologist HTL(ASCP) From mcauliff <@t> umdnj.edu Fri Mar 16 09:24:46 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Mar 16 09:23:35 2012 Subject: [Histonet] Staining GAGs in fetal membranes In-Reply-To: References: Message-ID: <4F634D2E.2020407@umdnj.edu> 1. Try an alcoholic fixative like formalin-alcohol-acetic acid 2. Try putting Safranin O in the fixative. Shepard and Mitchell, J. Ultrastruc. Res. 54:451-460, 1976. or same authors used Toluidine Blue O J. Histochem. Cytochem. 24:621-629, 1976. Geoff On 3/15/2012 8:41 PM, Jennifer Scholler wrote: > Hello, > > I am attempting to stain fetal membranes to look at the GAG content. My > advisor suggested Safronin O, which I tried on a preliminary set of slides > (hematoxylin, fast green, then safronin O). I did get some staining and saw > a band of red staining between the layers, as expected, but the staining is > not nearly as pronounced as in images of Safronin O stained tissues that I > have seen (some of this obviously may be due to imaging settings). Also, > after doing some reading, it seems that Alcian blue may be another option. > Can anyone with experience staining FMs or similar tissues suggest a stain > and protocol for this? > > Thanks! > > Regards, > > Jennifer Scholler > M.S. candidate > Mechanical Engineering > University of Colorado at Boulder > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Fri Mar 16 09:34:10 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 09:34:15 2012 Subject: [Histonet] Calponin In-Reply-To: <1331903646.10510.YahooMailClassic@web161806.mail.bf1.yahoo.com> Message-ID: <1331908450.66619.YahooMailClassic@web162101.mail.bf1.yahoo.com> DAKO monoclonal antibody at 1:1000 for 30 minutes, HIER citrate at pH6, colon cancer (+) case as control. Ren? J. --- On Fri, 3/16/12, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] Calponin To: histonet@lists.utsouthwestern.edu Date: Friday, March 16, 2012, 9:14 AM Hello Histonet Does anyone use Calponin stain? if so can you share the protocol with us? What do you use for epitope retrieval? Many thanks in advance. Chakib Boussahmain Histotechnologist HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 16 09:39:06 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 09:39:10 2012 Subject: [Histonet] Validation of new equipment In-Reply-To: <20120315141543.295dc6182df7e5cbb4f32bc101c30dcc.11e0648fb5.wbe@email15.secureserver.net> Message-ID: <1331908746.25916.YahooMailClassic@web162102.mail.bf1.yahoo.com> Your client and immediate supervisor is the pathologists, and s/he is also the one who has to answer directly to your inspecting authority, be it CAP or any other. Therefore, is s/he is satisfied?with the way the tissues you process in a new tissue processor turn out, that is sufficient. Any discrepancy of methodology with the inspecting authority let him/her to deal it with. Ren? J. --- On Thu, 3/15/12, Laurie@blufrogpath.com wrote: From: Laurie@blufrogpath.com Subject: [Histonet] Validation of new equipment To: "Histonet post" Date: Thursday, March 15, 2012, 5:15 PM ???If? I? am? validating? a new tissue processor, can I just process a v???ariety? of? tissues? and? have? the pathologist sign off that they are ???acce=? ptable,? or? do? I? need to do a parallel run with tissue on an ???established pro= cessor and compare the results? ??? ??? ???Laurie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laurie <@t> blufrogpath.com Fri Mar 16 10:53:01 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Fri Mar 16 10:53:10 2012 Subject: [Histonet] Medical Exams Message-ID: <20120316085301.295dc6182df7e5cbb4f32bc101c30dcc.96c77e39d7.wbe@email15.secureserver.net> Does anyone know if a pre-employment physical is required if you will be working with formalin? I have that stipulation in a procedure that info. Laurie From rjbuesa <@t> yahoo.com Fri Mar 16 11:08:31 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 11:08:34 2012 Subject: [Histonet] Medical Exams In-Reply-To: <20120316085301.295dc6182df7e5cbb4f32bc101c30dcc.96c77e39d7.wbe@email15.secureserver.net> Message-ID: <1331914111.94197.YahooMailClassic@web162105.mail.bf1.yahoo.com> A pre-employment physical is required in many hospitals regardless what you are going to be working with. It essentially refers to your employer health insurance. Ren? J. --- On Fri, 3/16/12, Laurie@blufrogpath.com wrote: From: Laurie@blufrogpath.com Subject: [Histonet] Medical Exams To: "Histonet post" Date: Friday, March 16, 2012, 11:53 AM ???Does? anyone? know? if? a pre-employment physical is required if you? ? will? be? working? with? formalin????I? have? that? stipulation? in? a ???procedure=? that? I've had for years, and I don't remember where I got ???that info. ???= ??? ???Laurie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Mar 16 11:25:29 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 16 11:35:14 2012 Subject: [Histonet] Calponin In-Reply-To: <1331908450.66619.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1331903646.10510.YahooMailClassic@web161806.mail.bf1.yahoo.com> <1331908450.66619.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: I think normal breast staining of myoepithial cells is the best control for this antibody. We use BioGenex's antibody (product # MU333-UC) at 1:200 on the Benchmark XT (CC1 mild, 8 mins primary antibody and ultraview DAB detection). Mark On Fri, Mar 16, 2012 at 7:34 AM, Rene J Buesa wrote: > DAKO monoclonal antibody at 1:1000 for 30 minutes, HIER citrate at pH6, > colon cancer (+) case as control. > Ren? J. > > --- On Fri, 3/16/12, Chakib Boussahmain wrote: > > > From: Chakib Boussahmain > Subject: [Histonet] Calponin > To: histonet@lists.utsouthwestern.edu > Date: Friday, March 16, 2012, 9:14 AM > > > Hello Histonet > Does anyone use Calponin stain? if so can you share the protocol with us? > What do you use for epitope retrieval? > Many thanks in advance. > Chakib Boussahmain > Histotechnologist > HTL(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Deb.Krol <@t> midmichigan.org Fri Mar 16 12:35:42 2012 From: Deb.Krol <@t> midmichigan.org (Deb.Krol@midmichigan.org) Date: Fri Mar 16 12:35:51 2012 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe this address. Deborah Krol Histology Section Head MidMichigan Medical Center 4005 Orchard Dr. Midland, Mi 48670 phone (989) 839-1836 fax (989) 839-3042 deb.krol@midmichigan.org ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From Luis.Chiriboga <@t> nyumc.org Fri Mar 16 12:40:28 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Fri Mar 16 12:41:25 2012 Subject: [Histonet] MMP2 Message-ID: <3E6798F00C9F494399E96B720ECD1429F943@MSGWCDCPMB22.nyumc.org> Hi Any recommendations for a MMP2 antibody for use in FFPE human tissues? Great weekend to all !! Luis From rsrichmond <@t> gmail.com Fri Mar 16 16:48:05 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Mar 16 16:48:08 2012 Subject: [Histonet] Re: PAS Diastase Message-ID: Jennifer MacDonald asked: >>At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this?<< If I want an undigested slide, I'll order it along with the PAS diastase. If I'm just trying to get the glycogen out of the way in a liver biopsy, I don't need the second slide. I've usually used the stain when I was looking for the PAS positive granules seen in hepatocytes in cirrhosis resulting from alpha-1 antitrypsin deficiency. Several years ago I got an anxious phone call from an internist taking care of a Baptist preacher who presented with cirrhosis of unknown cause. The pathologist I was filling in for (who should have known better), with no history at all, signed the biopsy out simply as "alcoholic cirrhosis". The internist pointed out that his patient had a somewhat low serum alpha-1 antitrypsin, in the heterozygous (MZ) range. I told him I had a stain for that. Sure enough, the man's hepatocytes contained the characteristic bright red granules. A brief review of the literature found a few reports of cirrhosis in AAT heterozygotes. I may have saved the man's job. Otherwise he would have had to become an Episcopalian like me. Bob Richmond Samurai Pathologist Knoxville TN From amber.mckenzie <@t> gastrodocs.net Fri Mar 16 16:58:46 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Mar 16 16:56:56 2012 Subject: [Histonet] water bath In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC115F02C1@JERRY.Gia.com> Is there a difference in using DI water vs tap water in the water bath? From tuyenmai77 <@t> yahoo.com Fri Mar 16 17:40:55 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Fri Mar 16 17:40:58 2012 Subject: [Histonet] chemical exposure Message-ID: <1331937655.53909.YahooMailClassic@web162806.mail.bf1.yahoo.com> I am wondering how much the tech exposes to the formalin and xylene during working with recycle equipment? my friend works with recycle equipment, and he still smell ?a lot of chemicals( he always wears special carbon filtered mask).Thank you,Mai Truong,Histotechnician, Duarte los Angeles California From tuyenmai77 <@t> yahoo.com Fri Mar 16 17:46:33 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Fri Mar 16 17:46:36 2012 Subject: [Histonet] Question about HT examination Message-ID: <1331937993.1379.YahooMailClassic@web162801.mail.bf1.yahoo.com> I don't have enough credits to renew my HT?license before the expired day. I wonder that I could re-take the HT examination before my license is expired ???Thank you,Mai Truong,Histotechnician Los Angeles California From rjbuesa <@t> yahoo.com Sat Mar 17 10:15:02 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 17 10:15:07 2012 Subject: [Histonet] water bath In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC115F02C1@JERRY.Gia.com> Message-ID: <1331997302.64923.YahooMailClassic@web162101.mail.bf1.yahoo.com> Yes. Tap water may contain suspended particle that can be transferred to the slide. Ren? J. --- On Fri, 3/16/12, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] water bath To: "histonet@lists.utsouthwestern.edu" Date: Friday, March 16, 2012, 5:58 PM Is there a difference in using DI water vs tap water in the water bath? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Mar 17 11:02:12 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 17 11:02:18 2012 Subject: AW: [Histonet] water bath In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC115F02C1@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC115F02C1@JERRY.Gia.com> Message-ID: <000001cd0457$51311750$f39345f0$@gmx.at> We use DI water in our waterbaths and have hardly problems with the adhesion of tissue on charged slides. - this may also be an advantage, because the counter-ions don't bind to each other. And the positive charge slide-surface isn't saturated. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amber McKenzie Gesendet: Freitag, 16. M?rz 2012 22:59 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] water bath Is there a difference in using DI water vs tap water in the water bath? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhotaks <@t> mcmaster.ca Sat Mar 17 12:35:01 2012 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Sat Mar 17 12:35:32 2012 Subject: [Histonet] MMP2 Message-ID: Hi Luis, I had good success with mouse monoclonal clone A-Gel2 VC2 from Neomarkers after HIER in citrate buffer. Please, check my paper: Lhotak S. et al, Clin Exp Metastasi 2000, 18(6): 463-70, for conditions, and for staining for other MMPs and TIMPS. It is a bit dated, though, there may be other antibodies available now. I don't work on MMPs anymore. Sarka Lhotak McMaster University, Hamilton, Ontario, Canada From tuyenmai77 <@t> yahoo.com Sat Mar 17 16:25:38 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Sat Mar 17 16:25:41 2012 Subject: [Histonet] Greetings, friend! Message-ID: <1332019538.95096.yint-ygo-j2me@web162802.mail.bf1.yahoo.com> Welcome, friend! http://decisaologistica.com.br/answers.php?ige=42&ybaqimyk=599&jxigorebe=16 From member <@t> linkedin.com Sun Mar 18 07:38:09 2012 From: member <@t> linkedin.com (erskine husbands via LinkedIn) Date: Sun Mar 18 07:38:12 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <882578095.2861434.1332074289560.JavaMail.app@ela4-bed82.prod> LinkedIn ------------ erskine husbands requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - erskine Accept invitation from erskine husbands http://www.linkedin.com/e/yvpgd1-gzy2mzyr-0/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I240147218_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYUcj8Td34Md399bSVQckF3iD9UbP0Rd3sVdPsPcPoLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=1NipppsJqmMR81 View invitation from erskine husbands http://www.linkedin.com/e/yvpgd1-gzy2mzyr-0/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I240147218_13/3cNnPwNczsQcj0QcAALqnpPbOYWrSlI/svi/?hs=false&tok=3PkMOncbymMR81 ------------------------------------------ Why might connecting with erskine husbands be a good idea? erskine husbands's connections could be useful to you: After accepting erskine husbands's invitation, check erskine husbands's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From lpwenk <@t> sbcglobal.net Sun Mar 18 11:02:50 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Mar 18 11:02:57 2012 Subject: [Histonet] adenovirus control Message-ID: <4B2BC87D94DC4E2EB1129BB7C3CCAE6A@HP2010> Anyone have an adenovirus control they are willing to spare? Or trade for something else you need? Contact me at work, please pwenk@beaumont.edu Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 From kdwyer3322 <@t> aol.com Sun Mar 18 12:43:56 2012 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Mar 18 12:44:05 2012 Subject: [Histonet] Texas Society for Histotechnology 2012 Convention in San Antonio TX Message-ID: <8CED345D3860A75-1C68-2A1B@webmail-m167.sysops.aol.com> Hello Histonet, It is not to late to register for the 2012 TSH meeting in fabulous San Antonio. There are still rooms available at the hotel the cutoff date is Thursday March 22, 2012. If you need a program or more information about the hotel please contact me (Kathy Dwyer) via this e-mail Texas Society for Histotechnology 35th Annual Symposium/Convention "Fiesta of Progress" April 13-15, 2012 Omni San Antonio Hotel 9821 Colonade Blvd. San Antonio, TX 78230 From gu.lang <@t> gmx.at Sun Mar 18 13:38:52 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Mar 18 13:38:59 2012 Subject: [Histonet] questionaire about fixation Message-ID: <003001cd0536$5e128fa0$1a37aee0$@gmx.at> Dear histonet! A few hours after submersion of a tissue-block (eg liver) in NBF, the block is cut across and we see the colour-change from brown to grey at the margins until a frontline. This frontline shows us: a) the distance of penetration of NBF; b) the distance of penetration AND addition of methylol-groups; c) the distance of penetration AND addition of methylol-groups AND (at least beginning) cross-linking. Do you see the issue? I would be happy, if you can help me to find a correct answer. Regards Gudrun Lang Biomed. Analytikerin Histolab Linz, Austria From bhartologist <@t> gmail.com Sun Mar 18 15:56:13 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sun Mar 18 15:56:16 2012 Subject: [Histonet] HT ASCP exam questions Message-ID: So, I just recently visited the ASCP website to look into the details of exam preparation and in that process, I discovered a time chart of when to apply for the exam depending on the date of completion for my program. It then says when you are prospectively eligible to sit for the exam after completing the application process. How accurate is this chart? How long after completing your program, for those of you that went through a program, were you able to sit for your exam? Also, Once you pass the exam are you certified and can you technically be hired on as a certified histotech somewhere or do you have to wait the 4 to 6 weeks for the certificate to arrive in the mail before you can work somewhere as a certified tech? From talulahgosh <@t> gmail.com Sun Mar 18 21:23:51 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Mar 18 21:23:55 2012 Subject: [Histonet] questionaire about fixation In-Reply-To: <003001cd0536$5e128fa0$1a37aee0$@gmx.at> References: <003001cd0536$5e128fa0$1a37aee0$@gmx.at> Message-ID: What the heck is a frontline? I tried to google it, but I got nothing useful. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang wrote: > Dear histonet! > > A few hours after submersion of a tissue-block (eg liver) in NBF, the block > is cut across and we see the colour-change from brown to grey at the > margins > until a frontline. > > This frontline shows us: > > a) the distance of penetration of NBF; > > b) the distance of penetration AND addition of methylol-groups; > > c) the distance of penetration AND addition of methylol-groups AND (at > least > beginning) cross-linking. > > > > Do you see the issue? > > I would be happy, if you can help me to find a correct answer. > > > > Regards > > Gudrun Lang > > > > Biomed. Analytikerin > > Histolab Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TroyerDA <@t> EVMS.EDU Mon Mar 19 09:40:23 2012 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Mon Mar 19 09:40:30 2012 Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc Message-ID: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer From bhartologist <@t> gmail.com Mon Mar 19 09:54:27 2012 From: bhartologist <@t> gmail.com (=?utf-8?B?YmhhcnRvbG9naXN0QGdtYWlsLmNvbQ==?=) Date: Mon Mar 19 09:54:36 2012 Subject: [Histonet] HT ASCP exam questions Message-ID: <4f6748a4.ca6a320a.7425.ffffcb09@mx.google.com> Sarah, you get your results right away now. You are told wether you passed or failed. But thanks for your help! :) From my HTC Amaze 4G on T-Mobile. The first nationwide 4G network From Amanda <@t> YPII.com Mon Mar 19 10:36:40 2012 From: Amanda <@t> YPII.com (Mandy O'Connor) Date: Mon Mar 19 10:36:51 2012 Subject: [Histonet] Fixation for breasts Message-ID: <8287D830EC5C904AA3E482C75773A03B0A0FCE76@EX1.YPIILab.YPII.com> Hey fellow histo nuts! Anyone have any good recommendations for fixing breast tissue? We were wondering about an alcoholic formalin but are unsure if it will hinder or mess up the SISH results. We usually end up re-processing the block again just to get a good slide that is semi readable. Thanks for any and all help! Mandy O'Connor HTL(ASCP) Yellowstone Pathology Instit. Billings, MT From trathborne <@t> somerset-healthcare.com Mon Mar 19 11:17:53 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Mar 19 11:18:28 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FA7CB7@SMCMAIL01.somerset-healthcare.com> You might currently be in violation for your storage conditions. It sounds as though the waste containers are NOT kept in a flammable proof cabinet at this time. Whichever room you decide to keep them in, this is something that you should consider. The fact that they're waste solutions does not decrease their flammability. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Monday, March 19, 2012 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From rjbuesa <@t> yahoo.com Mon Mar 19 11:26:22 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 11:26:31 2012 Subject: [Histonet] questionaire about fixation In-Reply-To: Message-ID: <1332174382.86725.YahooMailClassic@web162106.mail.bf1.yahoo.com> Emily: With formalin fixation, the formalin advances within the tissue in a way that you can macroscopically see up to where the formalin has penetrated. The appearance of the tissue changes in color and consistence. The change in appearance goes from the exterior to the interior of the tissue and that advance is seen as a line, hence "the front line" of the penetration. Ren? J. --- On Sun, 3/18/12, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] questionaire about fixation To: histonet@lists.utsouthwestern.edu Date: Sunday, March 18, 2012, 10:23 PM What the heck is a frontline? I tried to google it, but I got nothing useful. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang wrote: > Dear histonet! > > A few hours after submersion of a tissue-block (eg liver) in NBF, the block > is cut across and we see the colour-change from brown to grey at the > margins > until a frontline. > > This frontline shows us: > > a) the distance of penetration of NBF; > > b) the distance of penetration AND addition of methylol-groups; > > c) the distance of penetration AND addition of methylol-groups AND (at > least > beginning) cross-linking. > > > > Do you see the issue? > > I would be happy, if you can help me to find a correct answer. > > > > Regards > > Gudrun Lang > > > > Biomed. Analytikerin > > Histolab Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Mar 19 11:29:09 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Mar 19 11:29:18 2012 Subject: [Histonet] questionaire about fixation In-Reply-To: <1332174382.86725.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <1332174382.86725.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: So if you have a line, doesn't that mean your tissue isn't fixed properly? The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Mon, Mar 19, 2012 at 12:26 PM, Rene J Buesa wrote: > Emily: > With formalin fixation, the formalin advances within the tissue in a way > that you can macroscopically see up to where the formalin has penetrated. > The appearance of the tissue changes in color and consistence. The change > in appearance goes from the exterior to the interior of the tissue and that > advance is seen as a line, hence "the front line" of the penetration. > Ren? J. > > --- On *Sun, 3/18/12, Emily Sours * wrote: > > > From: Emily Sours > Subject: Re: [Histonet] questionaire about fixation > To: histonet@lists.utsouthwestern.edu > Date: Sunday, March 18, 2012, 10:23 PM > > > What the heck is a frontline? > I tried to google it, but I got nothing useful. > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang > > wrote: > > > Dear histonet! > > > > A few hours after submersion of a tissue-block (eg liver) in NBF, the > block > > is cut across and we see the colour-change from brown to grey at the > > margins > > until a frontline. > > > > This frontline shows us: > > > > a) the distance of penetration of NBF; > > > > b) the distance of penetration AND addition of methylol-groups; > > > > c) the distance of penetration AND addition of methylol-groups AND (at > > least > > beginning) cross-linking. > > > > > > > > Do you see the issue? > > > > I would be happy, if you can help me to find a correct answer. > > > > > > > > Regards > > > > Gudrun Lang > > > > > > > > Biomed. Analytikerin > > > > Histolab Linz, Austria > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Mon Mar 19 11:29:26 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 11:29:34 2012 Subject: [Histonet] Fixation for breasts In-Reply-To: <8287D830EC5C904AA3E482C75773A03B0A0FCE76@EX1.YPIILab.YPII.com> Message-ID: <1332174566.60758.YahooMailClassic@web162102.mail.bf1.yahoo.com> Cut thin slices of breast (3 mm thick or less), fix?them at 45?C in neutral buffered formalin for AT LEAST 36 hours to assure good fixation = cross-linking. If you do this you will?never need to reprocess a block. Ren? J. --- On Mon, 3/19/12, Mandy O'Connor wrote: From: Mandy O'Connor Subject: [Histonet] Fixation for breasts To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, March 19, 2012, 11:36 AM Hey fellow histo nuts!? Anyone have any good recommendations for fixing breast tissue?? We were wondering about an alcoholic formalin but are unsure if it will hinder or mess up the SISH results. We usually end up re-processing the block again just to get a good slide that is semi readable.? Thanks for any and all help! Mandy O'Connor? HTL(ASCP) Yellowstone Pathology Instit. Billings, MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Watson <@t> bms.com Mon Mar 19 11:31:06 2012 From: Linda.Watson <@t> bms.com (Watson, Linda) Date: Mon Mar 19 11:31:15 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FA7CB7@SMCMAIL01.somerset-healthcare.com> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> <3AD061FE740D464FAC7BF6B5CFB7570711FA7CB7@SMCMAIL01.somerset-healthcare.com> Message-ID: <351A66CE7FB11D40AB8AC8FE5559EC7E0C919D0270@ushpwbmsmmp008.one.ads.bms.com> Just thought I would chime in. All our flammable waste is stored in flammable approved waste containers and thus are located within the same lab space that we work in. You might want to look into using these types of containers. Linda :) >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni >Sent: Monday, March 19, 2012 12:18 PM >To: 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu >Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc > >You might currently be in violation for your storage conditions. It >sounds as though the waste containers are NOT kept in a flammable proof >cabinet at this time. Whichever room you decide to keep them in, this is >something that you should consider. The fact that they're waste >solutions does not decrease their flammability. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. >Sent: Monday, March 19, 2012 10:40 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc > >We were considering ways to tidy up a hospital pathology grossing room >where specimens are accessioned, grossed, and frozen sections performed. >There is one tissue processor in the room. >The room has only one doorway and has no windows. > >Several large plastic jugs of liquid waste (alcohols, xylene) are >retained within the room for weekly pick up by a waste disposal service. > >It was suggested that the waste jugs take up valuable floor space and >might be stored in an adjacent room, equally well ventilated, where >additional tissue processors are located. > >One of our laboratory supervisors indicated that the waste jugs could >not be stored outside the room because "waste liquids must be stored >within site of where they are generated". > >I believe the MSDS sheets/regulations were cited, but not sure. > >Is there experience with regulations that address this? It seems >counterintuitive to store flammable liquids in a room such as this with >a number of people there all day and evenings. Particularly if there is >an option of storing them in an adjacent room with releatively fewer >personnel. > >Dean Troyer > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE >This message and any included attachments are from Somerset Medical >Center >and are intended only for the addressee. The information contained in >this >message is confidential and may contain privileged, confidential, >proprietary and/or trade secret information entitled to protection >and/or >exemption from disclosure under applicable law. Unauthorized >forwarding, >printing, copying, distribution, or use of such information is strictly >prohibited and may be unlawful. If you are not the addressee, please >promptly delete this message and notify the sender of the delivery error >by e-mail or you may call Somerset Medical Center's computer Help Desk >at 908-685-2200, ext. 4050. > >Be sure to visit Somerset Medical Center's Web site - >www.somersetmedicalcenter.com - for the most up-to-date news, >event listings, health information and more. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From rjbuesa <@t> yahoo.com Mon Mar 19 11:35:28 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 11:35:33 2012 Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc In-Reply-To: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> Message-ID: <1332174928.24799.YahooMailClassic@web162104.mail.bf1.yahoo.com> What you describe is not an uncommon situation. What your supervisor said about storing wastes were they are generated and quoting MSDS for that is non sense. There is no regulation that says that and "throwing" the MSDS into the discussion, is just a "tactic" to give more weight to something that is not correct. On the other hand a closed room with just 1 door and no windows is not a good place to have dangerous chemicals. Another situation would be if in that room there was a fume hood or a good air extractor. That is what you need. MSDS usually include storage conditions in reference to space, but not about the origin of the wastes. Ren? J --- On Mon, 3/19/12, Troyer, Dean A. wrote: From: Troyer, Dean A. Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc To: histonet@lists.utsouthwestern.edu Date: Monday, March 19, 2012, 10:40 AM We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed.? There is one tissue processor in the room.? The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated".? I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this?? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings.? Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 19 11:37:15 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 11:37:22 2012 Subject: [Histonet] questionaire about fixation In-Reply-To: Message-ID: <1332175035.17513.YahooMailClassic@web162105.mail.bf1.yahoo.com> As long as you a line the tissue is NOT EVEN penetrated and much less fixed. Ren? J. --- On Mon, 3/19/12, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] questionaire about fixation To: histonet@lists.utsouthwestern.edu Date: Monday, March 19, 2012, 12:29 PM So if you have a line, doesn't that mean your tissue isn't fixed properly? The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Mon, Mar 19, 2012 at 12:26 PM, Rene J Buesa wrote: > Emily: > With formalin fixation, the formalin advances within the tissue in a way > that you can macroscopically see up to where the formalin has penetrated. > The appearance of the tissue changes in color and consistence. The change > in appearance goes from the exterior to the interior of the tissue and that > advance is seen as a line, hence "the front line" of the penetration. > Ren? J. > > --- On *Sun, 3/18/12, Emily Sours * wrote: > > > From: Emily Sours > Subject: Re: [Histonet] questionaire about fixation > To: histonet@lists.utsouthwestern.edu > Date: Sunday, March 18, 2012, 10:23 PM > > > What the heck is a frontline? > I tried to google it, but I got nothing useful. > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang > > wrote: > > > Dear histonet! > > > > A few hours after submersion of a tissue-block (eg liver) in NBF, the > block > > is cut across and we see the colour-change from brown to grey at the > > margins > > until a frontline. > > > > This frontline shows us: > > > > a) the distance of penetration of NBF; > > > > b) the distance of penetration AND addition of methylol-groups; > > > > c) the distance of penetration AND addition of methylol-groups AND (at > > least > > beginning) cross-linking. > > > > > > > > Do you see the issue? > > > > I would be happy, if you can help me to find a correct answer. > > > > > > > > Regards > > > > Gudrun Lang > > > > > > > > Biomed. Analytikerin > > > > Histolab Linz, Austria > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Mon Mar 19 13:13:29 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Mar 19 13:13:39 2012 Subject: [Histonet] hard vs. soft paraffin Message-ID: What constitutes "hard" paraffin vs. "soft" paraffin? Is it just melting point? What are the characteristics of hard and soft paraffins? How does water bath temperature play a role? Which is preferable for avoiding compression of tissue? thanks, Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From tkngflght <@t> yahoo.com Mon Mar 19 13:34:15 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Mar 19 13:34:18 2012 Subject: [Histonet] Local temp position in Louisville KY - DAY SHIFT Message-ID: <1332182055.93120.YahooMailNeo@web39405.mail.mud.yahoo.com> Hello guys- We just got a request for a?DAY SHIFT LOCAL HISTOTECH TEMP (not a traveler--someone who lives nearby).? There may be a?chance to be hired?as an employee depending on performance.? It's a 3 month assignment and I have to submit candidates?within a few days.? Will consider all experienced techs--call for more specifics.? It is near Lousville KY so all you in Ohio and Indiana who are close enough to commute please call! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From Joyce.Cline <@t> meritushealth.com Mon Mar 19 13:49:22 2012 From: Joyce.Cline <@t> meritushealth.com (Joyce Cline) Date: Mon Mar 19 13:51:50 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> Message-ID: <4A187D87076BF348A9A9FBAD6A6659BCF65A45@MHXCHCM.wchsys.org> Our waste is stored outside the building in a PIG unit that can hold two 55 gal drums leakage if necesary. We have a waste company pick up every 3 weeks. You cannot store on site more than 90 days flammable hazardous waste. Joyce Cline, H. T. (ASCP) Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@meritushealth.com ________________________________________ ______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From b427297 <@t> aol.com Mon Mar 19 13:52:57 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Mon Mar 19 13:53:11 2012 Subject: [Histonet] hard vs. soft paraffin In-Reply-To: References: Message-ID: <8CED418A221C883-D7C-1901F@webmail-d007.sysops.aol.com> The melting point of paraffin depends on the polymer % in the product. The higher the polymer content, the higher the melting point. Ideally, your paraffin hardness should mimic the tissue hardness, i.e., harder paraffins for bone, 'softer' paraffins for delicate biopsies. But, most busy labs don't have the time or the resources to use multiple paraffins, so they choose one best suited for multiple tissue types. What type of tissue are you having compression problems with? -----Original Message----- From: Clare Thornton To: 'histonet@lists.utsouthwestern.edu' Sent: Mon, Mar 19, 2012 1:14 pm Subject: [Histonet] hard vs. soft paraffin What constitutes "hard" paraffin vs. "soft" paraffin? Is it just melting point? What are the characteristics of hard and soft paraffins? How does water bath temperature play a role? Which is preferable for avoiding compression of tissue? thanks, Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Mar 19 13:58:45 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Mar 19 13:58:52 2012 Subject: [Histonet] Another local temp near INDIANAPOLIS - also dayshift Message-ID: <1332183525.85372.YahooMailNeo@web39406.mail.mud.yahoo.com> When it rains... ? Another LOCAL TEMP (not travel) outside of Indianapolis.? Also day shift with potential permanent opportunity. ? Will consider all experienced candidates...call me quickly! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From rjbuesa <@t> yahoo.com Mon Mar 19 15:46:42 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 15:46:49 2012 Subject: [Histonet] hard vs. soft paraffin In-Reply-To: Message-ID: <1332190002.64080.YahooMailClassic@web162103.mail.bf1.yahoo.com> Besides some additives that all paraffins have, "hard" paraffin is one that, as you point out, has a melting point (MP)?of about 63?C. There are even "harder" paraffins with MP of up to 70?C and usually are more white in color. The "normal" paraffin has a MP of about 53-55?C, and soft paraffins are of less than 50?C, usually about 43-45?C. The temperature of the water bath has nothing to do for paraffins above 50?C and is usually always at about 45?C. Sections of very soft paraffins should be extended in water baths at room temperature and usually are very difficult to work with. The harder the specimen, the higher the MP of the paraffin should be. Plant materials, specially twigs and branches to be cut transversely, require the hardest of the paraffins. Ideally the paraffin should have a hardness to coincide with that of the tissue. Ren? J. --- On Mon, 3/19/12, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] hard vs. soft paraffin To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, March 19, 2012, 2:13 PM What constitutes "hard" paraffin vs. "soft" paraffin?? Is it just melting point?? What are the characteristics of hard and soft paraffins?? How does water bath temperature play a role?? Which is preferable for avoiding compression of tissue? thanks, Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maui.histo <@t> gmail.com Mon Mar 19 16:33:12 2012 From: maui.histo <@t> gmail.com (Philip Muscarella) Date: Mon Mar 19 16:33:24 2012 Subject: [Histonet] hard vs. soft paraffin In-Reply-To: <1332190002.64080.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <1332190002.64080.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: <004901cd0617$e5ee0c10$b1ca2430$@gmail.com> Aside from the answers given, we have found that producing a continuous ribbon of paraffin mainly depends on the temperature of the block, and the blade. The hardness and size of the object may of course affect the ribbon, but if the object is small, and completely infiltrated, the effect is practically negligible. If the temperature of the knife and block is made sufficiently low, very thin sections can be cut without excessively compressing the object, regardless of the melting point of the paraffin. If the microtome is set for thinner sections without lowering the temperature of the knife and block, the sections are compressed more and more as they are made thinner, until the tissues are crushed out of all likeness to their original. The simplest way to cool the blade that we have found, is to simply spray the blade with fast freeze just before cutting. When problems with compression still exist aside from the obvious (cutting too fast, blocks not kept cold until just before cutting etc.), one might adjust the clearance angle of the blade. You can obtain a proper angle by systematically decreasing the clearance angle until you find an optimal position. Philip P. Muscarella, H.T. Histology Laboratory Dr. Malott M.D. - Dermatology Kihei, Maui, Hawaii -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 19, 2012 10:47 AM To: 'histonet@lists.utsouthwestern.edu'; Clare Thornton Subject: Re: [Histonet] hard vs. soft paraffin Besides some additives that all paraffins have, "hard" paraffin is one that, as you point out, has a melting point (MP)?of about 63?C. There are even "harder" paraffins with MP of up to 70?C and usually are more white in color. The "normal" paraffin has a MP of about 53-55?C, and soft paraffins are of less than 50?C, usually about 43-45?C. The temperature of the water bath has nothing to do for paraffins above 50?C and is usually always at about 45?C. Sections of very soft paraffins should be extended in water baths at room temperature and usually are very difficult to work with. The harder the specimen, the higher the MP of the paraffin should be. Plant materials, specially twigs and branches to be cut transversely, require the hardest of the paraffins. Ideally the paraffin should have a hardness to coincide with that of the tissue. Ren? J. --- On Mon, 3/19/12, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] hard vs. soft paraffin To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, March 19, 2012, 2:13 PM What constitutes "hard" paraffin vs. "soft" paraffin?? Is it just melting point?? What are the characteristics of hard and soft paraffins?? How does water bath temperature play a role?? Which is preferable for avoiding compression of tissue? thanks, Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Mar 19 17:01:00 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Mar 19 17:01:20 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A228D8@xmdb02.nch.kids> I hope that these are stored in flammable cupboards/rooms and that maximum allowable volumes are adhered to (that will definitely upset them). As to "waste liquids must be stored within sight of where they are generated"- I hope that all safety storage requirements are adhered to since they must take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Tuesday, 20 March 2012 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Mon Mar 19 17:03:22 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Mar 19 17:03:31 2012 Subject: [Histonet] RE: Fixation for breasts In-Reply-To: <8287D830EC5C904AA3E482C75773A03B0A0FCE76@EX1.YPIILab.YPII.com> References: <8287D830EC5C904AA3E482C75773A03B0A0FCE76@EX1.YPIILab.YPII.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A228E8@xmdb02.nch.kids> Try Microwaving blocks in 10% NBF or alcoholic formalin for 2 hours at 45oC. Works a treat with placentas Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mandy O'Connor Sent: Tuesday, 20 March 2012 2:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fixation for breasts Hey fellow histo nuts! Anyone have any good recommendations for fixing breast tissue? We were wondering about an alcoholic formalin but are unsure if it will hinder or mess up the SISH results. We usually end up re-processing the block again just to get a good slide that is semi readable. Thanks for any and all help! Mandy O'Connor HTL(ASCP) Yellowstone Pathology Instit. Billings, MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From dreynold <@t> mdanderson.org Mon Mar 19 17:07:22 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Mon Mar 19 17:10:12 2012 Subject: [Histonet] RE: Histonet Digest, Vol 100, Issue 23 In-Reply-To: <0b9b9807-6ba9-4c21-9ef3-fdc635b2c87e@DCPWPRTR03.mdanderson.edu> References: <0b9b9807-6ba9-4c21-9ef3-fdc635b2c87e@DCPWPRTR03.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F0230C844D3F9@DCPWVMBXC0VS3.mdanderson.edu> For MMP2 we use Rabbit anti-MMP2 - Abcam ab79781 with pepsin digestion 1:400 with Jackson goat anti-Rabbit HRP secondary. Message: 2 Date: Fri, 16 Mar 2012 17:40:28 +0000 From: "Chiriboga, Luis" Subject: [Histonet] MMP2 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3E6798F00C9F494399E96B720ECD1429F943@MSGWCDCPMB22.nyumc.org> Content-Type: text/plain; charset="us-ascii" Hi Any recommendations for a MMP2 antibody for use in FFPE human tissues? Great weekend to all !! Luis Date: Fri, 16 Mar 2012 21:58:46 +0000 From: Amber McKenzie Subject: [Histonet] water bath To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC115F02C1@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" Is there a difference in using DI water vs tap water in the water bath? From talulahgosh <@t> gmail.com Mon Mar 19 18:07:13 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Mar 19 18:07:18 2012 Subject: [Histonet] RE: Fixation for breasts In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A228E8@xmdb02.nch.kids> References: <8287D830EC5C904AA3E482C75773A03B0A0FCE76@EX1.YPIILab.YPII.com> <6D6BD1DE8A5571489398B392A38A715760A228E8@xmdb02.nch.kids> Message-ID: That subject heading is NOT safe for work. Even if you are talking about science. Emily (what? I know you were thinking it!!) The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Mon, Mar 19, 2012 at 6:03 PM, Tony Henwood (SCHN) < tony.henwood@health.nsw.gov.au> wrote: > Try Microwaving blocks in 10% NBF or alcoholic formalin for 2 hours at > 45oC. > > Works a treat with placentas > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mandy O'Connor > Sent: Tuesday, 20 March 2012 2:37 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Fixation for breasts > > Hey fellow histo nuts! Anyone have any good recommendations for fixing > breast tissue? We were wondering about an alcoholic formalin but are > unsure if it will hinder or mess up the SISH results. > > We usually end up re-processing the block again just to get a good slide > that is semi readable. Thanks for any and all help! > > Mandy O'Connor HTL(ASCP) > Yellowstone Pathology Instit. > Billings, MT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Diane.Tokugawa <@t> kp.org Mon Mar 19 19:09:18 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Mon Mar 19 19:46:05 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 03/19/2012 and will not return until 03/22/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From n.tonge <@t> epistem.co.uk Tue Mar 20 06:15:30 2012 From: n.tonge <@t> epistem.co.uk (Nicola Tonge) Date: Tue Mar 20 06:15:39 2012 Subject: [Histonet] Ammoniacal Silver Nitrate Solution Disposal Message-ID: <176C97AAA877D24798ED7376652D0FD815EFD11513@SRVEXCH02.epistem.local> Hello, Please could someone advise me on how to dispose of used ammoniacal silver nitrate solution? I have read you can precipitate with NaCl or HCl and then filter, but how much is required? Can the filter paper be disposed of in the yellow clinical waste bin or does it need removing by a specialist waste company. Also is there any way of knowing for certain that once precipitated and filtered the liquid is safe to gown down the drain? With kind regards, Nicola Tonge BSc Histology Manager Epistem Ltd 48, Grafton Street, Manchester, M13 9XX, UK. Office Tel. +44 (0) 161 606 7383 Fax: +44 (0) 161 606 7348 Epistem Limited Incorporated and registered in England and Wales under registration number: 06108621 Registered office: The Incubator Building, Grafton Street, Mancheser, M13 9XX. VAT registration number: 748 4927 83 This email and any attachments and files are confidential and are intended only for the addressee(s). Any review, onward transmission, disclosure or other use of this email or any attachments by any other person is prohibited. If you receive this email and are not an intended recipient please inform the sender immediately and delete this message, removing any copies from your system. Unless expressly stated otherwise, any opinions expressed in this email or any attachments transmitted with it are personal and do not constitute the opinions of EpiStem Holdings Plc or any subsidiary company (together "EpiStem"). Although emails are routinely screened for viruses, EpiStem does not accept any liability or responsibility for: (i) changes made to this email or any attachments transmitted with it after they were sent; or (ii) any viruses transmitted through this email or attachments transmitted with it. It is the responsibility of the recipient to ensure that the onward transmission, opening or use of this email or any files or attachments transmitted with it will not adversely affect its systems or data and the recipient should carry out such virus checks and other checks that it considers appropriate. For more information about EpiStem please visit: From kjgada <@t> gmail.com Tue Mar 20 06:50:38 2012 From: kjgada <@t> gmail.com (Komal Gada) Date: Tue Mar 20 06:50:48 2012 Subject: [Histonet] Per Diem availability Message-ID: Hello all, If anyone is in need of a per diem Histologist available on the weekends, please contact me. Thanks, Komal From kdwyer3322 <@t> aol.com Tue Mar 20 07:14:30 2012 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Tue Mar 20 07:14:41 2012 Subject: [Histonet] Texas Society for Histtechnology 2012 Meeting in San Antonio TX - Fiesta of Progress Message-ID: <8CED4AA22EBEB51-2FB0-12C12@webmail-m145.sysops.aol.com> Hello Histonet, It is not to late to register for the 2012 TSH meeting in fabulous San Antonio. There are still a few rooms available at the hotel the cutoff date to obtain a room has been extended to Monday March 26, 2012. If you need a program or more information about the hotel please contact me (Kathy Dwyer) via this e-mail Texas Society for Histotechnology 35th Annual Symposium/Convention "Fiesta of Progress" April 13-15, 2012 Omni San Antonio Hotel 9821 Colonade Blvd. San Antonio, TX 78230 From nto <@t> stowers.org Tue Mar 20 07:32:59 2012 From: nto <@t> stowers.org (Thomas, Nancy) Date: Tue Mar 20 07:33:11 2012 Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc In-Reply-To: <1332174928.24799.YahooMailClassic@web162104.mail.bf1.yahoo.com> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> <1332174928.24799.YahooMailClassic@web162104.mail.bf1.yahoo.com> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF99852DC3D@EXCHMB-02.stowers-institute.org> I don't think it is nonsense, because I have heard this before. I believe it comes from city or local fire dept. ordinances. I think your supervisor may have it turned around, though. It has been some time since I worked under these conditions, but we were able to keep a few gallons of waste in the lab, but larger amounts had to be kept away in properly ventilated areas. We had large drums down near the dock area of the hospital that we had to transfer waste to on a regular basis. Nancy Thomas Stowers Institute for Medical Research Kansas City, MO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 19, 2012 11:35 AM To: histonet@lists.utsouthwestern.edu; Dean A.Troyer Subject: Re: [Histonet] MSDS regarding waste alcohol, xylene, etc What you describe is not an uncommon situation. What your supervisor said about storing wastes were they are generated and quoting MSDS for that is non sense. There is no regulation that says that and "throwing" the MSDS into the discussion, is just a "tactic" to give more weight to something that is not correct. On the other hand a closed room with just 1 door and no windows is not a good place to have dangerous chemicals. Another situation would be if in that room there was a fume hood or a good air extractor. That is what you need. MSDS usually include storage conditions in reference to space, but not about the origin of the wastes. Ren? J --- On Mon, 3/19/12, Troyer, Dean A. wrote: From: Troyer, Dean A. Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc To: histonet@lists.utsouthwestern.edu Date: Monday, March 19, 2012, 10:40 AM We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed.? There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated".? I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this?? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings.? Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Mar 20 08:14:14 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Mar 20 08:14:27 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A228D8@xmdb02.nch.kids> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> <6D6BD1DE8A5571489398B392A38A715760A228D8@xmdb02.nch.kids> Message-ID: <090FA56107A969459F3941DDD5585C3A06917762@PHSX10MB10.partners.org> I thought I would add my 2 cents to this discussion. Our hazardous waste is regulated by the State and the MWRA (Mass Water Resource Association). Basically, we cannot throw anything down the drains. Therefore, we collect our alcohols, etc., label with hazardous waste labels, and store in special satellite accumulaton areas (usually hoods). We have an outside waste company that collects our waste weekly. We cannot leave hazardous waste containers (i.e. alcohol or xylene, etc.)or supplies of such outside a safety cabinet. Therefore, we are not allowed to stockpile solutions. Not sure what the regs are in your area. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, March 19, 2012 6:01 PM To: 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc I hope that these are stored in flammable cupboards/rooms and that maximum allowable volumes are adhered to (that will definitely upset them). As to "waste liquids must be stored within sight of where they are generated"- I hope that all safety storage requirements are adhered to since they must take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Tuesday, 20 March 2012 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From trathborne <@t> somerset-healthcare.com Tue Mar 20 08:35:13 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 20 08:36:06 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <090FA56107A969459F3941DDD5585C3A06917762@PHSX10MB10.partners.org> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> <6D6BD1DE8A5571489398B392A38A715760A228D8@xmdb02.nch.kids> <090FA56107A969459F3941DDD5585C3A06917762@PHSX10MB10.partners.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FB5163@SMCMAIL01.somerset-healthcare.com> You might be able to store larger volumes of these materials outside, if they are kept in an enclosed, secure (locked) storage area. This might require fewer visits by the waste company, if they collect more often than every 90 days now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, March 20, 2012 9:14 AM To: 'Tony Henwood (SCHN)'; 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc I thought I would add my 2 cents to this discussion. Our hazardous waste is regulated by the State and the MWRA (Mass Water Resource Association). Basically, we cannot throw anything down the drains. Therefore, we collect our alcohols, etc., label with hazardous waste labels, and store in special satellite accumulaton areas (usually hoods). We have an outside waste company that collects our waste weekly. We cannot leave hazardous waste containers (i.e. alcohol or xylene, etc.)or supplies of such outside a safety cabinet. Therefore, we are not allowed to stockpile solutions. Not sure what the regs are in your area. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, March 19, 2012 6:01 PM To: 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc I hope that these are stored in flammable cupboards/rooms and that maximum allowable volumes are adhered to (that will definitely upset them). As to "waste liquids must be stored within sight of where they are generated"- I hope that all safety storage requirements are adhered to since they must take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Tuesday, 20 March 2012 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From tp2 <@t> medicine.wisc.edu Tue Mar 20 11:35:01 2012 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Mar 20 11:35:21 2012 Subject: [Histonet] Cal-Ex for surface decalcification Message-ID: <4F686B65020000DF0000F0D1@gwmail.medicine.wisc.edu> Greetings Histonet, I have some mouse limbs to section. I've been told that they were decalcified for 3 hours prior to processing, but they may require some additional surface decal. I have some Cal-Ex on hand, but I've never used it before. Any tips that you ladies and gentlemen could give me would be appreciated. Thanks, Tom Pier From ces01 <@t> grh.org Tue Mar 20 11:58:28 2012 From: ces01 <@t> grh.org (Chase E. Shira) Date: Tue Mar 20 11:58:39 2012 Subject: [Histonet] When to toss reagents? Message-ID: When ordering new reagents, I have noticed many do not have an expiration date or a 'use by' date. How long after opening do you keep your reagents? We have several from the 70's!! Yikes! GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet Health Devices Achievement Award 2011 awarded by ECRI Institute GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From Kristopher.Kalleberg <@t> unilever.com Tue Mar 20 12:28:29 2012 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Mar 20 12:28:52 2012 Subject: [Histonet] Herovici stain Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0D9D3ADC@NTRSEVS30002.s3.ms.unilever.com> Is anyone familiar with Herovici stain? If so, does anyone know of a commercial vendor for this stain or does anyone have a protocol that they would be willing to share? I would like to stain some skin samples and compare collagen 1 and collagen 3 within the dermis. Thank you in advance. Kristopher L. Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 p 203.381.5765 f 203.381.5476 From wdesalvo.cac <@t> hotmail.com Tue Mar 20 12:48:28 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Mar 20 12:48:35 2012 Subject: [Histonet] When to toss reagents? In-Reply-To: References: Message-ID: Test the new reagent for performance and then apply a date label for 12 months from opening/use. Test the reagent at the end of the 12 month period for efficacy and if meets specification or standard, date for another 12 months. Continue the process until you demonstrate a performance issue. Discard when reagent does not perform as expected or meet standard. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 20 Mar 2012 09:58:28 -0700 > From: ces01@grh.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] When to toss reagents? > > When ordering new reagents, I have noticed many do not have an > expiration date or a 'use by' date. How long after opening do you keep > your reagents? We have several from the 70's!! Yikes! > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet > Health Devices Achievement Award 2011 awarded by ECRI Institute > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for return > or destruction of this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kjgada <@t> gmail.com Tue Mar 20 13:23:38 2012 From: kjgada <@t> gmail.com (Komal Gada) Date: Tue Mar 20 13:23:43 2012 Subject: [Histonet] Re: Per Diem availability In-Reply-To: References: Message-ID: Sorry for the hasty post. This is for the Orlando, FL and surrounding areas. Komal On Tue, Mar 20, 2012 at 7:50 AM, Komal Gada wrote: > Hello all, > > If anyone is in need of a per diem Histologist available on the weekends, > please contact me. > > Thanks, > Komal > From BGapinski <@t> pathgroup.com Tue Mar 20 13:48:05 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Tue Mar 20 13:48:18 2012 Subject: [Histonet] CLIA qualified Message-ID: <197CD0B02A81F94994A285C59C8AE05C084DEF7CD5@pgnexchange.pathgroup.com> Dear Histonians, I need a CLIA qualified HT to gross full time at our hospital. The problem is..... the money game. We need to create a new job, and we have no idea where to start with $/hr. A PA would be overkill for our job. Can anyone help us figure out what is fair compensation for a CLIA qualified technician? Respectfully, Bruce Gapinski HT(ASCP) Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From jaylundgren <@t> gmail.com Tue Mar 20 14:20:50 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 20 14:20:55 2012 Subject: [Histonet] CLIA qualified In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C084DEF7CD5@pgnexchange.pathgroup.com> References: <197CD0B02A81F94994A285C59C8AE05C084DEF7CD5@pgnexchange.pathgroup.com> Message-ID: Bruce, You need a recent college grad with the requisite hours of science courses. If a PA would be overkill, I suspect you need someone to just describe your small bx's and dump them in a cassette. In the facilities where I have worked where they have such a position, the "grossing tech" with a B.S. makes much less than a HT (ASCP) with a high school diploma. It is a position of great responsibility, with low pay. I would start in the neighborhood of $10- $12. an hour, but I have no idea what that equates to in California money. About 40-50% what you pay a starting Histotech. By the way, I would suggest you hire someone with no experience and train them yourselves. No experience equals no bad habits. Finding a person with the right attitude, who understands that the Histology lab literally deals with matters of life and death, is the hard part. Technique can be taught. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Mar 20 14:21:17 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 20 14:21:23 2012 Subject: AW: [Histonet] hard vs. soft paraffin In-Reply-To: References: Message-ID: <001401cd06ce$a031f2b0$e095d810$@gmx.at> The melting point usually depends on the length of the paraffin-chains. In the datasheet of paraffins, you will see something like C20-C35 for a melting point of 52-58?C. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Clare Thornton Gesendet: Montag, 19. M?rz 2012 19:13 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] hard vs. soft paraffin What constitutes "hard" paraffin vs. "soft" paraffin? Is it just melting point? What are the characteristics of hard and soft paraffins? How does water bath temperature play a role? Which is preferable for avoiding compression of tissue? thanks, Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Tue Mar 20 14:26:08 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Tue Mar 20 14:26:17 2012 Subject: [Histonet] CLIA qualified In-Reply-To: References: <197CD0B02A81F94994A285C59C8AE05C084DEF7CD5@pgnexchange.pathgroup.com> Message-ID: This is exactly what we have done in our lab. Our grossing techs stay for a few years until they leave for grad school, med school, official PA school, etc. We really enjoy the variety of people we have had working for us, and our proud of the places they have moved on to. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 20, 2012 2:21 PM To: Bruce Gapinski Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA qualified Bruce, You need a recent college grad with the requisite hours of science courses. If a PA would be overkill, I suspect you need someone to just describe your small bx's and dump them in a cassette. In the facilities where I have worked where they have such a position, the "grossing tech" with a B.S. makes much less than a HT (ASCP) with a high school diploma. It is a position of great responsibility, with low pay. I would start in the neighborhood of $10- $12. an hour, but I have no idea what that equates to in California money. About 40-50% what you pay a starting Histotech. By the way, I would suggest you hire someone with no experience and train them yourselves. No experience equals no bad habits. Finding a person with the right attitude, who understands that the Histology lab literally deals with matters of life and death, is the hard part. Technique can be taught. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Tue Mar 20 15:19:12 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Mar 20 15:19:07 2012 Subject: [Histonet] CLIA qualified In-Reply-To: References: <197CD0B02A81F94994A285C59C8AE05C084DEF7CD5@pgnexchange.pathgroup.com> Message-ID: The risk of that rate is the candidates that will likely apply might not have a strong skill set and if you find one that does, as soon as you train them, they will likely leave for more money, but at the end of the day, you will have to let the budget weigh in. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 20, 2012 3:21 PM To: Bruce Gapinski Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA qualified Bruce, You need a recent college grad with the requisite hours of science courses. If a PA would be overkill, I suspect you need someone to just describe your small bx's and dump them in a cassette. In the facilities where I have worked where they have such a position, the "grossing tech" with a B.S. makes much less than a HT (ASCP) with a high school diploma. It is a position of great responsibility, with low pay. I would start in the neighborhood of $10- $12. an hour, but I have no idea what that equates to in California money. About 40-50% what you pay a starting Histotech. By the way, I would suggest you hire someone with no experience and train them yourselves. No experience equals no bad habits. Finding a person with the right attitude, who understands that the Histology lab literally deals with matters of life and death, is the hard part. Technique can be taught. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From tony.henwood <@t> health.nsw.gov.au Tue Mar 20 17:36:20 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Mar 20 17:36:38 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <090FA56107A969459F3941DDD5585C3A06917762@PHSX10MB10.partners.org> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> <6D6BD1DE8A5571489398B392A38A715760A228D8@xmdb02.nch.kids> <090FA56107A969459F3941DDD5585C3A06917762@PHSX10MB10.partners.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A22DB6@xmdb02.nch.kids> Good, though I can see a conflict of interest between the water authorities and fire safety authorities. One say keep and dispose of appropriately. The other says if you keep then store it in minimum volumes and in appropriate safety cabinets. Now to keep both happy (as well as yourself) - that could be difficult Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Wednesday, 21 March 2012 12:14 AM To: Tony Henwood (SCHN); 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: RE: MSDS regarding waste alcohol, xylene, etc I thought I would add my 2 cents to this discussion. Our hazardous waste is regulated by the State and the MWRA (Mass Water Resource Association). Basically, we cannot throw anything down the drains. Therefore, we collect our alcohols, etc., label with hazardous waste labels, and store in special satellite accumulaton areas (usually hoods). We have an outside waste company that collects our waste weekly. We cannot leave hazardous waste containers (i.e. alcohol or xylene, etc.)or supplies of such outside a safety cabinet. Therefore, we are not allowed to stockpile solutions. Not sure what the regs are in your area. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, March 19, 2012 6:01 PM To: 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc I hope that these are stored in flammable cupboards/rooms and that maximum allowable volumes are adhered to (that will definitely upset them). As to "waste liquids must be stored within sight of where they are generated"- I hope that all safety storage requirements are adhered to since they must take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Tuesday, 20 March 2012 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From tony.henwood <@t> health.nsw.gov.au Tue Mar 20 17:38:44 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Mar 20 17:39:04 2012 Subject: [Histonet] RE: When to toss reagents? In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A22DEC@xmdb02.nch.kids> >From the seventies is not necessary a bad thing. Most you will find to be quite useable. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chase E. Shira Sent: Wednesday, 21 March 2012 3:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] When to toss reagents? When ordering new reagents, I have noticed many do not have an expiration date or a 'use by' date. How long after opening do you keep your reagents? We have several from the 70's!! Yikes! GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet Health Devices Achievement Award 2011 awarded by ECRI Institute GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From thisisann <@t> aol.com Tue Mar 20 18:58:26 2012 From: thisisann <@t> aol.com (Ann Angelo) Date: Tue Mar 20 18:58:32 2012 Subject: [Histonet] Block Storage Message-ID: <8CED50C79F9F24B-808-23BB@webmail-d158.sysops.aol.com> Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional component....or should they have the facility performing the professional component store them? Who is ultimately responsible? Ann From MSHERWOOD <@t> PARTNERS.ORG Wed Mar 21 08:00:31 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Wed Mar 21 08:00:45 2012 Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A22DB6@xmdb02.nch.kids> References: <9A3AF60951E04547B598B76A5CCC5C1803F8A969@vulcan.evms.net> <6D6BD1DE8A5571489398B392A38A715760A228D8@xmdb02.nch.kids> <090FA56107A969459F3941DDD5585C3A06917762@PHSX10MB10.partners.org> <6D6BD1DE8A5571489398B392A38A715760A22DB6@xmdb02.nch.kids> Message-ID: <090FA56107A969459F3941DDD5585C3A069179E8@PHSX10MB10.partners.org> We do keep "minimum volumes" of alcohol and xylene in the safety cabinet. But we can't order so much that some are stored on the floor outside the cabinet--keep just enough that you use in a reasonable time frame. We get alcohol in hospital, weekly, so it is not a problem. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Tuesday, March 20, 2012 6:36 PM To: Sherwood, Margaret; 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: RE: MSDS regarding waste alcohol, xylene, etc Good, though I can see a conflict of interest between the water authorities and fire safety authorities. One say keep and dispose of appropriately. The other says if you keep then store it in minimum volumes and in appropriate safety cabinets. Now to keep both happy (as well as yourself) - that could be difficult Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Wednesday, 21 March 2012 12:14 AM To: Tony Henwood (SCHN); 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: RE: MSDS regarding waste alcohol, xylene, etc I thought I would add my 2 cents to this discussion. Our hazardous waste is regulated by the State and the MWRA (Mass Water Resource Association). Basically, we cannot throw anything down the drains. Therefore, we collect our alcohols, etc., label with hazardous waste labels, and store in special satellite accumulaton areas (usually hoods). We have an outside waste company that collects our waste weekly. We cannot leave hazardous waste containers (i.e. alcohol or xylene, etc.)or supplies of such outside a safety cabinet. Therefore, we are not allowed to stockpile solutions. Not sure what the regs are in your area. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, March 19, 2012 6:01 PM To: 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc I hope that these are stored in flammable cupboards/rooms and that maximum allowable volumes are adhered to (that will definitely upset them). As to "waste liquids must be stored within sight of where they are generated"- I hope that all safety storage requirements are adhered to since they must take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Tuesday, 20 March 2012 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because "waste liquids must be stored within site of where they are generated". I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From flnails <@t> texaschildrens.org Wed Mar 21 08:00:39 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Mar 21 08:00:48 2012 Subject: [Histonet] Block Storage In-Reply-To: <8CED50C79F9F24B-808-23BB@webmail-d158.sysops.aol.com> References: <8CED50C79F9F24B-808-23BB@webmail-d158.sysops.aol.com> Message-ID: This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional component....or should they have the facility performing the professional component store them? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From caithesketh <@t> gmail.com Wed Mar 21 08:08:30 2012 From: caithesketh <@t> gmail.com (Caitlin Hesketh) Date: Wed Mar 21 08:08:39 2012 Subject: [Histonet] Thermo Scientific "Aquamount" procedure? Message-ID: I have a bottle of Thermo Scientific Aquamount and a path who is insisting that I start doing AEC chromogen on our immuno slides. We're still hand staining immunos, although we have a Benchmark Ultra now, the doctors are dragging their feet on reading the validation slides. Anyway, how exactly do I use the Aquamount? I seem to have lost the info sheet that came with it and can't find anything online. I used some aqueous mounting media ages ago and that involved just putting drops right on the slide and letting it harden. Or do I use it just like regular mountant, with a coverslip? Thanks so much for any help - Caitlin From kpilarc <@t> comcast.net Wed Mar 21 08:38:40 2012 From: kpilarc <@t> comcast.net (karen p) Date: Wed Mar 21 08:39:04 2012 Subject: [Histonet] Neurofilament - LFB combo -- problems Message-ID: <4F69D9E0.3090601@comcast.net> To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana Benchmark: I have been trying to stain a nerve (cross and longitudinal section) and it keeps falling off of the slide after the neurofilament stain. It's been cut onto a charged slide and has been baked 20 minutes prior to IHC staining. After IHC staining, we have rinsed in Dawn-water per protocol; by then the longitudinal section is peeling off the slide. I tried skipping the Dawn rinse and have gone to straight 95% alcohol and 1 of 4 sections survived on the slide (others fell off). Tissue problem - the nerve is well myelinated and fatty. If I drop the water bath temp too low, the nerve doesn't flatten well; if the temp is too high, it's shredding and spreading. Has anyone out there had this problem and resolved it? Or do you have a protocol that might have avoided it completely? Any suggestions would be appreciated. From arvidsonkristen <@t> yahoo.com Wed Mar 21 09:04:34 2012 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Mar 21 09:04:38 2012 Subject: [Histonet] Looking for Used cassette Printer Message-ID: <1332338674.54332.YahooMailClassic@web162106.mail.bf1.yahoo.com> I am looking for a used VCP cassette printer from Leica/Surgipath.? My print head finally went out.? Thanks. From rjbuesa <@t> yahoo.com Wed Mar 21 09:18:04 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 09:18:13 2012 Subject: [Histonet] Thermo Scientific "Aquamount" procedure? In-Reply-To: Message-ID: <1332339484.94313.YahooMailClassic@web162102.mail.bf1.yahoo.com> Use it as a regular mountant and coverslip. Ren? J. --- On Wed, 3/21/12, Caitlin Hesketh wrote: From: Caitlin Hesketh Subject: [Histonet] Thermo Scientific "Aquamount" procedure? To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:08 AM I have a bottle of Thermo Scientific Aquamount and a path who is insisting that I start doing AEC chromogen on our immuno slides. We're still hand staining immunos, although we have a Benchmark Ultra now, the doctors are dragging their feet on reading the validation slides. Anyway, how exactly do I use the Aquamount? I seem to have lost the info sheet that came with it and can't find anything online. I used some aqueous mounting media ages ago and that involved just putting drops right on the slide and letting it harden. Or do I use it just like regular mountant, with a coverslip? Thanks so much for any help - Caitlin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 21 09:20:11 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 09:20:15 2012 Subject: [Histonet] Block Storage In-Reply-To: Message-ID: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com> Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component? on if they do not perform the professional component....or should they have the facility performing the professional component store them?? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laurie <@t> blufrogpath.com Wed Mar 21 09:27:52 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Wed Mar 21 09:28:02 2012 Subject: [Histonet] Water Quality Message-ID: <20120321072752.295dc6182df7e5cbb4f32bc101c30dcc.60f5e7114d.wbe@email15.secureserver.net> In regards to CAP's Lab General Checklist questions regardin quality: Is deionized water consid Water)? Is water testin small labs test f Would anyone be willing to share their procedure for Water Quality for a small, private pathology lab? Thank Laurie Colbert From rjbuesa <@t> yahoo.com Wed Mar 21 09:34:36 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 09:34:44 2012 Subject: [Histonet] Water Quality In-Reply-To: <20120321072752.295dc6182df7e5cbb4f32bc101c30dcc.60f5e7114d.wbe@email15.secureserver.net> Message-ID: <1332340476.48118.YahooMailClassic@web162105.mail.bf1.yahoo.com> When you introduce the words "reagent quality" it means that a chemical manufacturer has done specific tests to assure that the water complies with such designation. Deionized water is usable?for solutions preparations but the issue resides in the deionizer that has to be tested as to its performance. No "small laboratory" has the? conditions required to perform microbiology cultures, nor it is really necessary to do under normal working histology conditions. If these are new CAP requirements it seems to me that CAP is going beyond reasonable requirements. Ren? J. --- On Wed, 3/21/12, Laurie@blufrogpath.com wrote: From: Laurie@blufrogpath.com Subject: [Histonet] Water Quality To: "Histonet post" Date: Wednesday, March 21, 2012, 10:27 AM ???In? regards to CAP's Lab General Checklist questions regardin= g water ???quality: ??? ??? ???Is? deionized? water? consid=? ered? CLRW (Clinical Laboratory Reagent ???Water)? ??? ???Is? water? testin=? g? necessary? for? a Histology Lab?? If so, how do ???small labs test f= or resisitivity and perform microbiology cultures? ??? ??? ???Would? anyone? be? willing to share their procedure for Water Quality???for a small, private pathology lab? ??? ??? ???Thank= s, ??? ???Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Mar 21 09:40:23 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Mar 21 09:40:35 2012 Subject: [Histonet] Water Quality In-Reply-To: <1332340476.48118.YahooMailClassic@web162105.mail.bf1.yahoo.com> References: <20120321072752.295dc6182df7e5cbb4f32bc101c30dcc.60f5e7114d.wbe@email15.secureserver.net> <1332340476.48118.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E1C3243@evcspmbx3.ads.northwestern.edu> Our DI tanks are provided and serviced by Seimens. They do the testing. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 9:35 AM To: Histonet post; Laurie@blufrogpath.com Subject: Re: [Histonet] Water Quality When you introduce the words "reagent quality" it means that a chemical manufacturer has done specific tests to assure that the water complies with such designation. Deionized water is usable?for solutions preparations but the issue resides in the deionizer that has to be tested as to its performance. No "small laboratory" has the? conditions required to perform microbiology cultures, nor it is really necessary to do under normal working histology conditions. If these are new CAP requirements it seems to me that CAP is going beyond reasonable requirements. Ren? J. --- On Wed, 3/21/12, Laurie@blufrogpath.com wrote: From: Laurie@blufrogpath.com Subject: [Histonet] Water Quality To: "Histonet post" Date: Wednesday, March 21, 2012, 10:27 AM ???In? regards to CAP's Lab General Checklist questions regardin= g water ???quality: ??? ??? ???Is? deionized? water? consid=? ered? CLRW (Clinical Laboratory Reagent ???Water)? ??? ???Is? water? testin=? g? necessary? for? a Histology Lab?? If so, how do ???small labs test f= or resisitivity and perform microbiology cultures? ??? ??? ???Would? anyone? be? willing to share their procedure for Water Quality???for a small, private pathology lab? ??? ??? ???Thank= s, ??? ???Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Wed Mar 21 10:01:46 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Mar 21 10:02:13 2012 Subject: [Histonet] Histotechnologist (Lead) on the 3rd shift needed in Ft. Myers, FL Message-ID: Allied Search Partners is currently looking for a qualified applicant for a Histotechnologist for a 3rd shift Lead position available in a Fort Myers, FL laboratory. Position: Histotechnologist Schedule: 3rd shift/full time permanent position Summary: Perform a variety of routine and specialized histology techniques and procedures. Embedding, Microtomy, Grossing, Processing, and H&E staining Special Staining Equipment maintenance Requirements: FL Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science Benefits: Competitive salaries, Health, Dental, Life & Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To Apply: Please send resume to brannon@alliedsearchpartners.com -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From tajibade <@t> echd.org Wed Mar 21 10:16:36 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Wed Mar 21 10:16:45 2012 Subject: [Histonet] April fool's prank Message-ID: Any ideal for April fool's prank? Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From flnails <@t> texaschildrens.org Wed Mar 21 10:19:28 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Mar 21 10:19:40 2012 Subject: [Histonet] Block Storage In-Reply-To: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: Rene I think this question is eluding to a Physician owned lab and who is responsible for storing the blocks not how long they are retained? ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, March 21, 2012 9:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; Nails, Felton Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional component....or should they have the facility performing the professional component store them? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From cpyse <@t> x-celllab.com Wed Mar 21 10:25:13 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Mar 21 10:25:22 2012 Subject: [Histonet] Block Storage-another question In-Reply-To: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: <001b01cd0776$d0a0b1e0$71e215a0$@com> If the pod lab is in NJ and the "reading" lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component? on if they do not perform the professional component....or should they have the facility performing the professional component store them?? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Mar 21 10:25:37 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Wed Mar 21 10:25:46 2012 Subject: [Histonet] Re: Neurofilament - LFB combo -- problems Message-ID: <9F3CFEE76E51B64991C7485270890B4009F10FD2@EX5.lj.gnf.org> Karen writes: To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana Benchmark: I have been trying to stain a nerve (cross and longitudinal section) and it keeps falling off of the slide after the neurofilament stain. It's been cut onto a charged slide and has been baked 20 minutes prior to IHC staining. After IHC staining, we have rinsed in Dawn-water per protocol; by then the longitudinal section is peeling off the slide. I tried skipping the Dawn rinse and have gone to straight 95% alcohol and 1 of 4 sections survived on the slide (others fell off). Tissue problem - the nerve is well myelinated and fatty. If I drop the water bath temp too low, the nerve doesn't flatten well; if the temp is too high, it's shredding and spreading. Has anyone out there had this problem and resolved it? Or do you have a protocol that might have avoided it completely? Any suggestions would be appreciated. Hi Karen, I don't know if any of this will help you, but here are a few suggestions. You can treat this tissue like we would brain or bone, after the water is drained off the section, lay the slides flat, tissue side up, on a slide warmer at lower temp, maybe 37-45 degrees C. You can try overnight and see if this helps. You can also try using Haupt's adhesive treated slides, they use this for plastic sections and bone and it tends to work pretty well. You can buy it commercially from Dorn and Hart, or you can make your own. The recipe is on the internet. Maybe use a combination of both? Try baking it a little longer too, 1-2 hours at 60 degrees if the above doesn't work. I know how frustrating this can be! Good luck. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From sbreeden <@t> nmda.nmsu.edu Wed Mar 21 10:27:11 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 21 10:27:30 2012 Subject: [Histonet] April fool's prank In-Reply-To: References: Message-ID: <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! From kdboydhisto <@t> yahoo.com Wed Mar 21 10:28:39 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Mar 21 10:28:46 2012 Subject: [Histonet] Re: Histonet Digest, Vol 100, Issue 27 Message-ID: <1332343719.67148.YahooMailNeo@web125806.mail.ne1.yahoo.com> I would be very careful when you say you need a CLIA certified tech to do gross (high complexity testing). You actually need someone who meets the CLIA requirements to perform High Complexity Testing. You can have a tech who is certified, but they may not meet the educational requirements.?That person?MUST?have 60 semester hours, 24 of those?in science, 6 hours must be Biology and 6 must be Chemistry and documented training in the area of high complexity testing. It has NOTHING to with certification, unfortunately.? Kelly D. Boyd, BS, HTL (ASCP) From patjnm <@t> gwumc.edu Wed Mar 21 10:27:07 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Wed Mar 21 10:35:13 2012 Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? Message-ID: <4F69BB0B.DB55.001F.1@gwumc.edu> Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From Jonathan.Cremer <@t> med.kuleuven.be Wed Mar 21 10:36:44 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Mar 21 10:36:54 2012 Subject: [Histonet] April fool's prank In-Reply-To: <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> References: , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: What a horrifying idea to put charcoal -or any material that produces dust- on a microscope. I like the blank slides idea though. ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Breeden, Sara [sbreeden@nmda.nmsu.edu] Verzonden: woensdag 21 maart 2012 16:27 Aan: Tunde Ajibade; histonet-bounces@lists.utsouthwestern.edu CC: histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] April fool's prank I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karabou76 <@t> hotmail.com Wed Mar 21 10:42:59 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Wed Mar 21 10:43:08 2012 Subject: [Histonet] April fool's prank In-Reply-To: <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> References: , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee > Date: Wed, 21 Mar 2012 09:27:11 -0600 > From: sbreeden@nmda.nmsu.edu > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > CC: histonet@lists.utsouthwestern.edu > > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > consume Mass Quantities of the stuff???). A tray of completely blank > slides, all properly numbered for the day's work? > > Feed me some good ones, too. I'm down to my last week and I need to be > remembered when I'm gone so they won't call me to do p.r.n.! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Mar 21 10:53:14 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Mar 21 10:55:26 2012 Subject: [Histonet] Block Storage-another question In-Reply-To: <001b01cd0776$d0a0b1e0$71e215a0$@com> References: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com>, <001b01cd0776$d0a0b1e0$71e215a0$@com> Message-ID: I would think that if NYS is involved in anyway you have to hold those blocks for 20 years. When they inspect you do they inspect both labs? Do both labs hold a NYS permit? For cetain I would call NYS before you discard anything. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse [cpyse@x-celllab.com] Sent: Wednesday, March 21, 2012 11:25 AM To: 'Rene J Buesa'; 'Ann Angelo'; histonet@lists.utsouthwestern.edu; 'FeltonNails' Subject: RE: [Histonet] Block Storage-another question If the pod lab is in NJ and the "reading" lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional component....or should they have the facility performing the professional component store them? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SLB <@t> stowers.org Wed Mar 21 11:13:00 2012 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Wed Mar 21 11:13:09 2012 Subject: [Histonet] April fool's prank In-Reply-To: References: , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org> Please tell me you are kidding about all this stuff!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee > Date: Wed, 21 Mar 2012 09:27:11 -0600 > From: sbreeden@nmda.nmsu.edu > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > CC: histonet@lists.utsouthwestern.edu > > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > consume Mass Quantities of the stuff???). A tray of completely blank > slides, all properly numbered for the day's work? > > Feed me some good ones, too. I'm down to my last week and I need to > be remembered when I'm gone so they won't call me to do p.r.n.! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Wed Mar 21 11:16:43 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Mar 21 11:16:52 2012 Subject: [Histonet] April fool's prank In-Reply-To: <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: There are a few I remember from the past: Telling the pathologists that the processor has been delayed or stopped running in the alcohols. If you ever want to see them go bonkers - that one will do it. Loosening or taking out of bulbs from microscopes - not a very nice thing to do but I've heard of it. Also make sure that the back up bulbs have been safely secured elsewhere. No I've not done this......but I'm told a pathologist or tech without their microscope can be pretty intense. There is always the sick call thing - but it has grown sort of old. Having a pathologist read a fake slide. We used an oak leaf - made out all the paperwork and all of it tied into 'woody' things. Had an unsuspecting green tech gross it in and so on. It's amazing what you can pull off on newbies. Put Vaseline on the microscope stage - slides will go 'slip sliddn' away as they try to adjust x/y positions. Tape or block (pennies sometimes work) their top center desk drawer shut - they can't open other drawers if that one won't open. (or am I dating myself). Replace sharp pencils and pens with dull pencils(stubs if possible) and pens that don't work or are difficult. There is always replacing their red pen ink with blue or black ink. A favorite is replacing a keyboard or loosening a connection on their computer or dictation machine. If it's a 'dead' keyboard they don't know what to do initially - if it's a wrong keyboard it's even more fun. PC vs. MAC in the beginning they don't even look they just don't know why it won't work. It's before they've had the chance for their 5th cup of coffee. For the techs - Setting the microtome to 0 on older ones or changing the auto to not advance. I had trouble with the automated ones but found I could rig it just right. If ever practice made perfect - this was one, because I learned alot on my own about automation there. Have fun and be creative. Lab work can be pretty intense - some good clean laughs break the intensity. On Wed, Mar 21, 2012 at 11:27 AM, Breeden, Sara wrote: > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > consume Mass Quantities of the stuff???). A tray of completely blank > slides, all properly numbered for the day's work? > > Feed me some good ones, too. I'm down to my last week and I need to be > remembered when I'm gone so they won't call me to do p.r.n.! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DStillings <@t> unipathdx.com Wed Mar 21 11:17:23 2012 From: DStillings <@t> unipathdx.com (Donella Stillings) Date: Wed Mar 21 11:16:56 2012 Subject: [Histonet] April fool's prank In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org> References: , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org> Message-ID: <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> I second that. I hope you are kidding! Hm. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 9:13 AM To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee > Date: Wed, 21 Mar 2012 09:27:11 -0600 > From: sbreeden@nmda.nmsu.edu > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > CC: histonet@lists.utsouthwestern.edu > > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > consume Mass Quantities of the stuff???). A tray of completely blank > slides, all properly numbered for the day's work? > > Feed me some good ones, too. I'm down to my last week and I need to > be remembered when I'm gone so they won't call me to do p.r.n.! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karabou76 <@t> hotmail.com Wed Mar 21 11:18:57 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Wed Mar 21 11:19:01 2012 Subject: [Histonet] April fool's prank In-Reply-To: <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> References: , , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu>, , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org>, <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> Message-ID: Nope. a fun work environment breeds better workers. > From: DStillings@unipathdx.com > To: SLB@stowers.org; karabou76@hotmail.com; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > Date: Wed, 21 Mar 2012 16:17:23 +0000 > > I second that. I hope you are kidding! Hm. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon > Sent: Wednesday, March 21, 2012 9:13 AM > To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > Please tell me you are kidding about all this stuff!!!!! > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee > Sent: Wednesday, March 21, 2012 10:43 AM > To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > > While I'm new at histology, I am a master of pranks. > Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) > > 1. fill their desk space with packing peanuts > > 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny > > 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) > > 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. > > 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. > > 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. > > 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. > > Enjoy! > Kara Lee > > > > Date: Wed, 21 Mar 2012 09:27:11 -0600 > > From: sbreeden@nmda.nmsu.edu > > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > > Subject: RE: [Histonet] April fool's prank > > CC: histonet@lists.utsouthwestern.edu > > > > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > > consume Mass Quantities of the stuff???). A tray of completely blank > > slides, all properly numbered for the day's work? > > > > Feed me some good ones, too. I'm down to my last week and I need to > > be remembered when I'm gone so they won't call me to do p.r.n.! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhadley <@t> iupui.edu Wed Mar 21 11:23:50 2012 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Wed Mar 21 11:24:00 2012 Subject: [Histonet] April fool's prank In-Reply-To: References: , , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu>, , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org>, <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F2DD858BE@IU-MSSG-MBX110.ads.iu.edu> I'm with you Kara. No harm no foul. Lee Ann Baldridge IUSM Indpls., IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 12:19 PM To: dstillings@unipathdx.com; slb@stowers.org; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Nope. a fun work environment breeds better workers. > From: DStillings@unipathdx.com > To: SLB@stowers.org; karabou76@hotmail.com; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > Date: Wed, 21 Mar 2012 16:17:23 +0000 > > I second that. I hope you are kidding! Hm. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon > Sent: Wednesday, March 21, 2012 9:13 AM > To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > Please tell me you are kidding about all this stuff!!!!! > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee > Sent: Wednesday, March 21, 2012 10:43 AM > To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > > While I'm new at histology, I am a master of pranks. > Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) > > 1. fill their desk space with packing peanuts > > 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny > > 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) > > 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. > > 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. > > 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. > > 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. > > Enjoy! > Kara Lee > > > > Date: Wed, 21 Mar 2012 09:27:11 -0600 > > From: sbreeden@nmda.nmsu.edu > > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > > Subject: RE: [Histonet] April fool's prank > > CC: histonet@lists.utsouthwestern.edu > > > > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > > consume Mass Quantities of the stuff???). A tray of completely blank > > slides, all properly numbered for the day's work? > > > > Feed me some good ones, too. I'm down to my last week and I need to > > be remembered when I'm gone so they won't call me to do p.r.n.! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 21 11:23:26 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 21 11:24:07 2012 Subject: [Histonet] April fool's prank In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org> References: , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu> <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org> Message-ID: I'm loving it! Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 12:13 PM To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee > Date: Wed, 21 Mar 2012 09:27:11 -0600 > From: sbreeden@nmda.nmsu.edu > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > CC: histonet@lists.utsouthwestern.edu > > I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's > eyepieces. Vaseline on the coffee cup handles (don't all pathologists > consume Mass Quantities of the stuff???). A tray of completely blank > slides, all properly numbered for the day's work? > > Feed me some good ones, too. I'm down to my last week and I need to > be remembered when I'm gone so they won't call me to do p.r.n.! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Wed Mar 21 11:31:26 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Mar 21 11:34:46 2012 Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? In-Reply-To: <4F69BB0B.DB55.001F.1@gwumc.edu> References: <4F69BB0B.DB55.001F.1@gwumc.edu> Message-ID: <493CAA64F203E14E8823737B9EE0E25F0921436403@EXCHMB01.isis.local> I like the DAKO antibody diluent. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Madary Sent: Wednesday, March 21, 2012 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu From NMP <@t> stowers.org Wed Mar 21 11:35:28 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Mar 21 11:35:41 2012 Subject: [Histonet] April fool's prank In-Reply-To: References: , , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu>, , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org>, <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF997F37206@EXCHMB-02.stowers-institute.org> I guess everyone's idea of 'fun' can be different. A lot of these things sound like a lot of work to 'clean up'. Funny is different than making a mess to me--just 1 cent worth :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 11:19 AM To: dstillings@unipathdx.com; Beckham, Sharon; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Nope. a fun work environment breeds better workers. > From: DStillings@unipathdx.com > To: SLB@stowers.org; karabou76@hotmail.com; sbreeden@nmda.nmsu.edu; > tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > Date: Wed, 21 Mar 2012 16:17:23 +0000 > > I second that. I hope you are kidding! Hm. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Beckham, Sharon > Sent: Wednesday, March 21, 2012 9:13 AM > To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; > histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > Please tell me you are kidding about all this stuff!!!!! > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara > Lee > Sent: Wednesday, March 21, 2012 10:43 AM > To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; > histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > > While I'm new at histology, I am a master of pranks. > Here's a list of some fun ones I've done to co-workers over that > years...that haven't gotten me fired or killed yet ;) > > 1. fill their desk space with packing peanuts > > 2. If you have the time, post-it note their entire office, office > wall, ceiling and desk with the multi colored post-it notes...it's > pretty and funny > > 3. Find a dead keyboard in the IT department. Take it home, fill the > areas around the keys with dirt and chia-pet seeds. Wait for them to > sprout. Come in early in the morning and replace someones keyboard > with this one (don't actually plug it into the computer) > > 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. > > 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. > > 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. > > 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. > > Enjoy! > Kara Lee > > > > Date: Wed, 21 Mar 2012 09:27:11 -0600 > > From: sbreeden@nmda.nmsu.edu > > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > > Subject: RE: [Histonet] April fool's prank > > CC: histonet@lists.utsouthwestern.edu > > > > I always liked the M*A*S*H* one with the charcoal (?) on the > > 'scope's eyepieces. Vaseline on the coffee cup handles (don't all > > pathologists consume Mass Quantities of the stuff???). A tray of > > completely blank slides, all properly numbered for the day's work? > > > > Feed me some good ones, too. I'm down to my last week and I need to > > be remembered when I'm gone so they won't call me to do p.r.n.! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Wed Mar 21 11:44:17 2012 From: thisisann <@t> aol.com (Ann Angelo) Date: Wed Mar 21 11:44:33 2012 Subject: [Histonet] Block Storage-another question In-Reply-To: <001b01cd0776$d0a0b1e0$71e215a0$@com> References: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com> <001b01cd0776$d0a0b1e0$71e215a0$@com> Message-ID: <8CED598FDCD98E4-C78-294E7@Webmail-d109.sysops.aol.com> Thank you for your input. We always go by the most strict regulating body which is NY and we save our blocks 20 years. Ann -----Original Message----- From: Cynthia Pyse To: 'Rene J Buesa' ; 'Ann Angelo' ; histonet ; 'FeltonNails' Sent: Wed, Mar 21, 2012 11:25 am Subject: RE: [Histonet] Block Storage-another question If the pod lab is in NJ and the "reading" lab is in NY, which guide lines do ou follow. NYS requires us to save our blocks for 20 years. Due to storage ssues, I would rather the pod lab store the blocks. indy -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa ent: Wednesday, March 21, 2012 10:20 AM o: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails ubject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are tored during different periods of time before being discarded. I used to tore them for 9 years. en? J. --- On Wed, 3/21/12, Nails, Felton wrote: rom: Nails, Felton ubject: RE: [Histonet] Block Storage o: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" histonet@lists.utsouthwestern.edu> ate: Wednesday, March 21, 2012, 9:00 AM his is a very sticky issue, when I setup inhouse labs I always present the rgument that during inspections the lab that produces a report should have ccess to all test material which includes blocks and slides per the CAP hecklist. nn trying approaching it from that angle. -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo ent: Tuesday, March 20, 2012 6:58 PM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they erform Technical component on if they do not perform the professional omponent....or should they have the facility performing the professional omponent store them? Who is ultimately responsible? Ann ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ ONFIDENTIALITY NOTICE: he information in this e-mail may be confidential and/or rivileged. If you are not the intended recipient or an uthorized representative of the intended recipient, you re hereby notified that any review, dissemination, or opying of this e-mail and its attachments, if any, or he information contained herein is prohibited. If you ave received this e-mail in error, please immediately otify the sender by return e-mail and delete this e-mail rom your computer system. Thank you. _____________________________________________________________________ _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Wed Mar 21 11:45:43 2012 From: thisisann <@t> aol.com (Ann Angelo) Date: Wed Mar 21 11:46:03 2012 Subject: [Histonet] Block Storage-another question In-Reply-To: References: <1332339611.55842.YahooMailClassic@web162103.mail.bf1.yahoo.com>, <001b01cd0776$d0a0b1e0$71e215a0$@com> Message-ID: <8CED59930997B2D-C78-2952B@Webmail-d109.sysops.aol.com> I know to hold them for 20 years, but where? At the lab where the blocks were processed or at the lab where the slides were read? Ann -----Original Message----- From: McMahon, Loralee A To: Cynthia Pyse ; 'Rene J Buesa' ; 'Ann Angelo' ; histonet ; 'FeltonNails' Sent: Wed, Mar 21, 2012 11:55 am Subject: RE: [Histonet] Block Storage-another question I would think that if NYS is involved in anyway you have to hold those blocks or 20 years. When they inspect you do they inspect both labs? Do both labs old a NYS permit? For cetain I would call NYS before you discard anything. Loralee McMahon, HTL (ASCP) mmunohistochemistry Supervisor trong Memorial Hospital epartment of Surgical Pathology 585) 275-7210 _______________________________________ rom: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Cynthia Pyse [cpyse@x-celllab.com] ent: Wednesday, March 21, 2012 11:25 AM o: 'Rene J Buesa'; 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails' ubject: RE: [Histonet] Block Storage-another question If the pod lab is in NJ and the "reading" lab is in NY, which guide lines do ou follow. NYS requires us to save our blocks for 20 years. Due to storage ssues, I would rather the pod lab store the blocks. indy -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa ent: Wednesday, March 21, 2012 10:20 AM o: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails ubject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are tored during different periods of time before being discarded. I used to tore them for 9 years. en? J. --- On Wed, 3/21/12, Nails, Felton wrote: rom: Nails, Felton ubject: RE: [Histonet] Block Storage o: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" histonet@lists.utsouthwestern.edu> ate: Wednesday, March 21, 2012, 9:00 AM his is a very sticky issue, when I setup inhouse labs I always present the rgument that during inspections the lab that produces a report should have ccess to all test material which includes blocks and slides per the CAP hecklist. nn trying approaching it from that angle. -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo ent: Tuesday, March 20, 2012 6:58 PM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they erform Technical component on if they do not perform the professional omponent....or should they have the facility performing the professional omponent store them? Who is ultimately responsible? Ann ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ ONFIDENTIALITY NOTICE: he information in this e-mail may be confidential and/or rivileged. If you are not the intended recipient or an uthorized representative of the intended recipient, you re hereby notified that any review, dissemination, or opying of this e-mail and its attachments, if any, or he information contained herein is prohibited. If you ave received this e-mail in error, please immediately otify the sender by return e-mail and delete this e-mail rom your computer system. Thank you. _____________________________________________________________________ _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From las.charette <@t> comcast.net Wed Mar 21 11:59:48 2012 From: las.charette <@t> comcast.net (LaSalette Charette) Date: Wed Mar 21 12:00:11 2012 Subject: [Histonet] Fixatives for breast Message-ID: What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette From one_angel_secret <@t> yahoo.com Wed Mar 21 12:14:14 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Mar 21 12:14:19 2012 Subject: [Histonet] Block Storage In-Reply-To: <8CED50C79F9F24B-808-23BB@webmail-d158.sysops.aol.com> References: <8CED50C79F9F24B-808-23BB@webmail-d158.sysops.aol.com> Message-ID: <1332350054.68518.YahooMailNeo@web112310.mail.gq1.yahoo.com> You dont send your blocks out when you send your slides to be interpreted for the professional component do you? Ive never seen that done personally.What I have seen is the lab that created the blocks stored themand just sent slides out to be read. And if it were me in this position I'd say keep for 20 years since NY requires that, because the Pathologist (In NY)?reading out your cases may be called upon to produce other testing etc for those cases he/she read out in NY and the material would need to be available. Just my 2cents worth. Kim Donadio ________________________________ From: Ann Angelo To: histonet@lists.utsouthwestern.edu Sent: Tuesday, March 20, 2012 7:58 PM Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component? on if they do not perform the professional component....or should they have the facility performing the professional component store them?? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Wed Mar 21 12:29:59 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Mar 21 12:30:01 2012 Subject: [Histonet] Certified techs/high complexity testing Message-ID: <1332350999.40465.YahooMailNeo@web125804.mail.ne1.yahoo.com> I would be very careful when you say you need a CLIA certified tech to do gross (high complexity testing). You actually need someone who meets the CLIA requirements to perform High Complexity Testing. You can have a tech who is certified, but they may not meet the educational requirements. That person MUST have 60 semester hours, 24 of those in science, 6 hours must be Biology and 6 must be Chemistry and documented training in the area of high complexity testing. It has NOTHING to with certification, unfortunately. Kelly D. Boyd, BS, HTL (ASCP) From david.l.krull <@t> gsk.com Wed Mar 21 12:30:20 2012 From: david.l.krull <@t> gsk.com (David Krull) Date: Wed Mar 21 12:30:32 2012 Subject: [Histonet] Re: commercial antibody diluent with carrier proteins Message-ID: I have used the Renaissance Background Reducing Diluent from Biocare Medical. It works great on human and rodent tissues. Biocare also had a wide selection of diluents and blocking reagents when used together can significantly reduce background and enhance staining intensity. Best Regards, Dave Investigative Pathology GlaxoSmithKline From sbreeden <@t> nmda.nmsu.edu Wed Mar 21 12:39:59 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 21 12:40:17 2012 Subject: [Histonet] April fool's prank In-Reply-To: References: , , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu>, , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org>, <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> Message-ID: <02C099024072804EA34F5906BAC30A413F2002@nmdamailsvr.nmda.ad.nmsu.edu> Amen!! From Caroline.Pratt <@t> uphs.upenn.edu Wed Mar 21 12:40:49 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Mar 21 12:40:48 2012 Subject: [Histonet] Certified techs/high complexity testing In-Reply-To: <1332350999.40465.YahooMailNeo@web125804.mail.ne1.yahoo.com> References: <1332350999.40465.YahooMailNeo@web125804.mail.ne1.yahoo.com> Message-ID: Exactly, we are trying to educate our staff on this currently. It is important to understand because the regulatory bodies do focus on this issue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, March 21, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Certified techs/high complexity testing I would be very careful when you say you need a CLIA certified tech to do gross (high complexity testing). You actually need someone who meets the CLIA requirements to perform High Complexity Testing. You can have a tech who is certified, but they may not meet the educational requirements. That person MUST have 60 semester hours, 24 of those in science, 6 hours must be Biology and 6 must be Chemistry and documented training in the area of high complexity testing. It has NOTHING to with certification, unfortunately. Kelly D. Boyd, BS, HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From shu-cheng.chen <@t> merck.com Wed Mar 21 13:55:06 2012 From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng) Date: Wed Mar 21 13:55:13 2012 Subject: [Histonet] Cryostat sectioning Message-ID: <5D62649615FAA6478F801A08D10E518566F0F99ADF@USCTMXP51003.merck.com> Hi, We are searching for a fee for service lab experienced in cryostat sectioning of animal and human tissues. Any recommendation is appreciated. Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Wed Mar 21 15:26:11 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 15:26:16 2012 Subject: [Histonet] Block Storage In-Reply-To: Message-ID: <1332361571.88228.YahooMailClassic@web162103.mail.bf1.yahoo.com> Regardless where the blocks are generated, that private lab has to follow state regulations. That will be a legal?matter related to any possible law suit after an alleged misdiagnosis. If the private lab has no available space, there are companies that can store blocks and slides for as long they are paid to retain them. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Rene J Buesa'" , "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 11:19 AM Rene I think this question is eluding to a Physician owned lab and who is responsible for storing the blocks not how long they are retained? From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, March 21, 2012 9:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; Nails, Felton Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component? on if they do not perform the professional component....or should they have the facility performing the professional component store them?? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From rjbuesa <@t> yahoo.com Wed Mar 21 15:27:20 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 15:27:24 2012 Subject: [Histonet] Block Storage-another question In-Reply-To: <001b01cd0776$d0a0b1e0$71e215a0$@com> Message-ID: <1332361640.16900.YahooMailClassic@web162102.mail.bf1.yahoo.com> Both but especially the place where the diagnosis takes place. Ren? J. --- On Wed, 3/21/12, Cynthia Pyse wrote: From: Cynthia Pyse Subject: RE: [Histonet] Block Storage-another question To: "'Rene J Buesa'" , "'Ann Angelo'" , histonet@lists.utsouthwestern.edu, "'FeltonNails'" Date: Wednesday, March 21, 2012, 11:25 AM If the pod lab is in NJ and the "reading" lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. Ren? J. --- On Wed, 3/21/12, Nails, Felton wrote: From: Nails, Felton Subject: RE: [Histonet] Block Storage To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component? on if they do not perform the professional component....or should they have the facility performing the professional component store them?? Who is ultimately responsible? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 21 15:28:48 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 15:28:52 2012 Subject: [Histonet] Fixatives for breast In-Reply-To: Message-ID: <1332361728.46781.YahooMailClassic@web162105.mail.bf1.yahoo.com> Neutral buffered formalin for 36 hours at room temperature. Ren? J. --- On Wed, 3/21/12, LaSalette Charette wrote: From: LaSalette Charette Subject: [Histonet] Fixatives for breast To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 21, 2012, 12:59 PM What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Wed Mar 21 15:28:18 2012 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Mar 21 15:29:00 2012 Subject: [Histonet] antigen retrieval Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00271984C65F@CVM77.vetmed.wsu.edu> To those with the Biocare intelliPATH system, Is the antigen retrieval part of the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom Truscott From rjbuesa <@t> yahoo.com Wed Mar 21 15:43:01 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 15:43:05 2012 Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? In-Reply-To: <4F69BB0B.DB55.001F.1@gwumc.edu> Message-ID: <1332362581.97793.YahooMailClassic@web162104.mail.bf1.yahoo.com> Joseph: ALL commercial antibody diluent carriers ?contain the same ingredients (bovine albumen, Tween 20 and sodium azide as preservative in pH7 PBS buffer). ? Every 100 mL of antibody diluent contains: Bovine albumen --------- 1 mL PBS pH7 buffer --------- 99 mL Tween 20 ----------------- 50 ?L sodium azide ----------100 mg ? Why don't you prepare it yourself? That is what I used to do and saved a lot of money! Ren? J. --- On Wed, 3/21/12, Joseph Madary wrote: From: Joseph Madary Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 11:27 AM Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Mar 21 15:43:59 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Mar 21 15:44:04 2012 Subject: [Histonet] RE: antigen retrieval In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A00271984C65F@CVM77.vetmed.wsu.edu> References: <9EF5279EBDFE6E4FB6605E8F183A00271984C65F@CVM77.vetmed.wsu.edu> Message-ID: Tim- Antigen retrieval is off-line... you need the decloaker or other device. Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Wednesday, March 21, 2012 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antigen retrieval To those with the Biocare intelliPATH system, Is the antigen retrieval part of the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom Truscott _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pathlocums <@t> gmail.com Wed Mar 21 16:11:09 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Mar 21 16:11:16 2012 Subject: [Histonet] Block Storage-another question In-Reply-To: <1332361640.16900.YahooMailClassic@web162102.mail.bf1.yahoo.com> References: <001b01cd0776$d0a0b1e0$71e215a0$@com> <1332361640.16900.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: Fellow Histonet Subscribers, I have been noticing a LOT of misinformation being passed around this site, much coming from one respondent in particular. This is very troubling, as our position in healthcare, and the work we do, is so very critical to patient care and patient outcomes. I would hope that before responding to questions, people either know what they are talking about, or make it clear that it is opinion they are expressing, and not necessarily fact. Perhaps including some reference, or web link to information is practical at times. Here I have listed below responses to some questions that are outright false, and the information presented, if followed, in some instances may put a lab in jeopardy when inspected. It also appears that we have techs from marine biology centers, and plant histology labs answering questions pertaining to medicine. I am not sure how appropriate that is. This is, of course, just my opinion. *In Response to the question of where to store blocks this was an answer:* ?Both but especially the place where the diagnosis takes place.? It is not possible to store blocks in BOTH locations. Why would you do that? And ?especially the place where the diagnosis takes place? is incorrect. The logical place to keep the blocks is where the histology is performed. If space does not permit long term storage, rent a facility that is compliant with your state requirements for doing so. As far as time to keep blocks, CLIA and CAP have different requirements. Check with your State to see if they are even more stringent than CAP. Here is what CLIA, and CAP require: FROM CLIA: Sec. 493.1105 Standard: Retention requirements (a)(3)(ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. FROM CAP: Retention of Laboratory Records and Materials: *Surgical Pathology (including bone marrows)* Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years *In Response to Water Quality this response was noted:* ?No "small laboratory" has the conditions required to perform microbiology cultures? This is an inaccurate statement. Size of a lab, and ability to perform work in a sterile environment are not at all related. When responding to questions as important as breast fixation times, it would be helpful to include information like what I included immediately below ? furthermore, as all of you know, breast tissue fixes differently depending upon several factors, especially fat content, and thickness of the sections. The question of best fixation time is not an answerable question ? it is specific to the case itself. Is it cores we are talking about? Is it a dense fibrous lumpectomy? Is it a fatty mastectomy? There is no perfect answer to that question. *What are the changes made to minimum fixation times?* The minimum fixation time for HER2 has been clarified and we recommend that samples for HER2 testing be fixed a minimum of 6 hours. The original statement that smaller samples can be fixed for less than 6 hours is not supported by the literature. We recommend that sample for HER2 testing be fixed a minimum of 6 hours regardless of sample size. *WWhat about changes to maximum fixation times? The HER2 fixation time of 6-48 hours is not consistent with that of the ER/PgR fixation time of 6-72 hours.* We are unable to find evidence to support increasing the HER2 fixation time and therefore recommendations for fixation times in neutral buffered formalin are unchanged (6-48 hours for HER2 and 6-72 hours for ER/PgR). The data about the stability of ER and PgR at intervals of 48-72 hours suggest that changing this interval for HER2 testing will not result in adverse testing results. However, there is a lack of specific published studies for HER2 IHC that included specimens with low levels of HER2 expression that would be more vulnerable to fixation time changes. *What are the changes made to minimum fixation times?* The minimum fixation time for HER2 has been clarified and we recommend that samples for HER2 testing be fixed a minimum of 6 hours. The original statement that smaller samples can be fixed for less than 6 hours is not supported by the literature. We recommend that sample for HER2 testing be fixed a minimum of 6 hours regardless of sample size. On Wed, Mar 21, 2012 at 1:27 PM, Rene J Buesa wrote: > Both but especially the place where the diagnosis takes place. > Ren? J. > > --- On Wed, 3/21/12, Cynthia Pyse wrote: > > > From: Cynthia Pyse > Subject: RE: [Histonet] Block Storage-another question > To: "'Rene J Buesa'" , "'Ann Angelo'" < > thisisann@aol.com>, histonet@lists.utsouthwestern.edu, "'FeltonNails'" < > flnails@texaschildrens.org> > Date: Wednesday, March 21, 2012, 11:25 AM > > > If the pod lab is in NJ and the "reading" lab is in NY, which guide lines > do > you follow. NYS requires us to save our blocks for 20 years. Due to storage > issues, I would rather the pod lab store the blocks. > Cindy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Wednesday, March 21, 2012 10:20 AM > To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails > Subject: RE: [Histonet] Block Storage > > Depending on the state regulations and the laboratory policies,blocks are > stored during different periods of time before being discarded. I used to > store them for 9 years. > Ren? J. > > --- On Wed, 3/21/12, Nails, Felton wrote: > > > From: Nails, Felton > Subject: RE: [Histonet] Block Storage > To: "'Ann Angelo'" , "histonet@lists.utsouthwestern.edu > " > > Date: Wednesday, March 21, 2012, 9:00 AM > > > This is a very sticky issue, when I setup inhouse labs I always present the > argument that during inspections the lab that produces a report should have > access to all test material which includes blocks and slides per the CAP > checklist. > Ann trying approaching it from that angle. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo > Sent: Tuesday, March 20, 2012 6:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block Storage > > Does anyone know if a laboratory in NJ is required to keep the blocks they > perform Technical component on if they do not perform the professional > component....or should they have the facility performing the professional > component store them? Who is ultimately responsible? Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From plucas <@t> biopath.org Wed Mar 21 16:15:43 2012 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Mar 21 16:13:13 2012 Subject: [Histonet] Job Opening orange county Message-ID: We have a part-time histotech position available and anyone who is interested, please send me your resume by fax or email. The work days are Tuesday through Saturday starting at 5 am. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 Fax: 714-755-2984 From pathlocums <@t> gmail.com Wed Mar 21 16:26:13 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Mar 21 16:26:18 2012 Subject: [Histonet] Per Diem position in Los Angeles Message-ID: Dear Histonet Colleagues, We have a per diem position to fill for a new lab in the Beverly Hills area of Los Angeles. Looking for an experienced histotech, ASCP certified, to fill in when our staff is short. We will not be opening for a couple of months, however I would really like to get a couple of good techs on board with us to cover in times of need. It will be infrequent, but somewhat flexible with your current work schedule, and the pay will be quite competitive. If you are interested, please send your resume to me at: david@blufrogpath.com Thank you. Have a great day! -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 21 22:16:55 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Mar 21 22:16:43 2012 Subject: [Histonet] RE: April fool's prank In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9179C9F73A5@UTHCMS1.uthouston.edu> Not to put a damper on your fun but something to think about. I have played my April fools pranks and also been at the other end on many occasions. Let us not forget in these high stress times that perceptions vary considerably and what one person considers as a harmless and amusing prank to them may be embarrassing or even mentally damaging to another. If you have seen "Revenge of the Geeks" with its team bullying you know what I mean. Please think long and hard before you start any pranks. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade [tajibade@echd.org] Sent: Wednesday, March 21, 2012 10:16 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] April fool's prank Any ideal for April fool's prank? Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hmarlatt26 <@t> gmail.com Wed Mar 21 22:24:36 2012 From: hmarlatt26 <@t> gmail.com (heather marlatt) Date: Wed Mar 21 22:24:41 2012 Subject: [Histonet] re: april fools prank Message-ID: I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) From tuyenmai77 <@t> yahoo.com Thu Mar 22 04:31:36 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Thu Mar 22 04:31:44 2012 Subject: [Histonet] Fwd: Message-ID: <1332408696.49634.yint-ygo-j2me@web162801.mail.bf1.yahoo.com> Hi, friend! http://luk-art.com/notice.php?bequvuvu=86&evutjdegid=392&ogynjgus=27 From SLB <@t> stowers.org Thu Mar 22 07:14:41 2012 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Thu Mar 22 07:14:51 2012 Subject: [Histonet] re: april fools prank In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. Sharon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of heather marlatt Sent: Wednesday, March 21, 2012 10:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: april fools prank I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie <@t> marshfieldclinic.org Thu Mar 22 07:22:13 2012 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Thu Mar 22 07:22:31 2012 Subject: [Histonet] Just for fun Message-ID: <201203221222.q2MCMNqV024248@mailhost3.mfldclin.edu> Got this off the net: Can't attest to it's accuracy. April Fool's Day History The history of April Fool's Day or All Fool's Day is uncertain, but the current thinking is that it began around 1582 in France with the reform of the calendar under Charles IX. The Gregorian Calendar was introduced, and New Year's Day was moved from March 25 - April 1 (new year's week) to January 1. Communication traveled slowly in those days and some people were only informed of the change several years later. Still others, who were more rebellious refused to acknowledge the change and continued to celebrate on the last day of the former celebration, April 1. These people were labeled "fools" by the general populace, were subject to ridicule and sent on "fool errands," sent invitations to nonexistent parties and had other practical jokes played upon them. The butts of these pranks became known as a "poisson d'avril" or "April fish" because a young naive fish is easily caught. In addition, one common practice was to hook a paper fish on the back of someone as a joke. This harassment evolved over time and a custom of prank-playing continue on the first day of April. This tradition eventually spread elsewhere like to Britain and Scotland in the 18th century and was introduced to the American colonies by the English and the French. Because of this spread to other countries, April Fool's Day has taken on an international flavor with each country celebrating the holiday in its own way. In Scotland, for instance, April Fool's Day is devoted to spoofs involving the buttocks and as such is called Taily Day. The butts of these jokes are known as April 'Gowk', another name for cuckoo bird. The origins of the "Kick Me" sign can be traced back to the Scottish observance. In England, jokes are played only in the morning. Fools are called 'gobs' or 'gobby' and the victim of a joke is called a 'noodle.' It was considered back luck to play a practical joke on someone after noon. In Rome, the holiday is known as Festival of Hilaria, celebrating the resurrection of the god Attis, is on March 25 and is also referred to as "Roman Laughing Day." In Portugal, April Fool's Day falls on the Sunday and Monday before lent. In this celebration, many people throw flour at their friends. The Huli Festival is celebrated on March 31 in India. People play jokes on one another and smear colors on one another celebrating the arrival of Spring. So, no matter where you happen to be in the world on April 1, don't be surprised if April fools fall playfully upon you. ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Thu Mar 22 08:13:54 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 22 08:14:03 2012 Subject: [Histonet] Just for fun In-Reply-To: <201203221222.q2MCMNqV024248@mailhost3.mfldclin.edu> Message-ID: <1332422034.417.YahooMailClassic@web162101.mail.bf1.yahoo.com> Laurie: I really appreciate your historical note unknown to me and of especial personal?significance because I am a "legitimate" April fool being born?in April 1, 1934 which was Easter Sunday that year. Thanks Ren? J. --- On Thu, 3/22/12, King, Laurie wrote: From: King, Laurie Subject: [Histonet] Just for fun To: "histonet@lists.utsouthwestern.edu" Date: Thursday, March 22, 2012, 8:22 AM Got this off the net: Can't attest to it's accuracy. April Fool's Day History The history of April Fool's Day or All Fool's Day is uncertain, but the current thinking is that it began around 1582 in France with the reform of the calendar under Charles IX. The Gregorian Calendar was introduced, and New Year's Day was moved from March 25 - April 1 (new year's week) to January 1. Communication traveled slowly in those days and some people were only informed of the change several years later. Still others, who were more rebellious refused to acknowledge the change and continued to celebrate on the last day of the former celebration, April 1.? These people were labeled "fools" by the general populace, were subject to ridicule and sent on "fool errands," sent invitations to nonexistent parties and had other practical jokes played upon them. The butts of these pranks became known as a "poisson d'avril" or "April fish" because a young naive fish is easily caught. In addition, one common practice was to hook a paper fish on the back of someone as a joke. This harassment evolved over time and a custom of prank-playing continue on the first day of April. This tradition eventually spread elsewhere like to Britain and Scotland in the 18th century and was introduced to the American colonies by the English and the French. Because of this spread to other countries, April Fool's Day has taken on an international flavor with each country celebrating the holiday in its own way. In Scotland, for instance, April Fool's Day is devoted to spoofs involving the buttocks and as such is called Taily Day. The butts of these jokes are known as April 'Gowk', another name for cuckoo bird. The origins of the "Kick Me" sign can be traced back to the Scottish observance. In England, jokes are played only in the morning. Fools are called 'gobs' or 'gobby' and the victim of a joke is called a 'noodle.' It was considered back luck to play a practical joke on someone after noon. In Rome, the holiday is known as Festival of Hilaria, celebrating the resurrection of the god Attis, is on March 25 and is also referred to as "Roman Laughing Day." In Portugal, April Fool's Day falls on the Sunday and Monday before lent. In this celebration, many people throw flour at their friends. The Huli Festival is celebrated on March 31 in India. People play jokes on one another and smear colors on one another celebrating the arrival of Spring. So, no matter where you happen to be in the world on April 1, don't be surprised if April fools fall playfully upon you. ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Mar 22 09:45:46 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Mar 22 09:46:03 2012 Subject: [Histonet] RE: Antiboey Diluents In-Reply-To: <1332362581.97793.YahooMailClassic@web162104.mail.bf1.yahoo.com> References: <4F69BB0B.DB55.001F.1@gwumc.edu> <1332362581.97793.YahooMailClassic@web162104.mail.bf1.yahoo.com> Message-ID: Not all antibody diluents contain BSA - proprietary carrier proteins include synthetic polymers. Not all antibody diluents are buffered with PBS - some are buffered with TBS. If you make your antibody diluents in-house, be very selective about the BSA source, especially if you work with veterinary tissues. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 2:43 PM To: histonet@lists.utsouthwestern.edu; Joseph Madary Subject: Re: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? Joseph: ALL commercial antibody diluent carriers ?contain the same ingredients (bovine albumen, Tween 20 and sodium azide as preservative in pH7 PBS buffer). ? Every 100 mL of antibody diluent contains: Bovine albumen --------- 1 mL PBS pH7 buffer --------- 99 mL Tween 20 ----------------- 50 ?L sodium azide ----------100 mg ? Why don't you prepare it yourself? That is what I used to do and saved a lot of money! Ren? J. --- On Wed, 3/21/12, Joseph Madary wrote: From: Joseph Madary Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 11:27 AM Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Mar 22 10:26:16 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Mar 22 10:26:37 2012 Subject: [Histonet] April fool's prank In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF997F37206@EXCHMB-02.stowers-institute.org> References: , , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu>, , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org>, <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> <2C40E43D1F7A56408C4463FD245DDDF997F37206@EXCHMB-02.stowers-institute.org> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE974@MCINFRWEM003.ucsfmedicalcenter.org> Some people played a May 5 - Cinco de Mayo joke on one of our staff of Mexican heritage. He always came in early to embed and on May 5 one year they set up a cassette recorder on a timer to start playing mariachi music at 5:30 am. Then several of them came into the lab with the fake mustaches, big sombero's, woven ponchos and shakers and singing along with the music. The guy was laughing so hard he couldn't stand up. In fact he kept laughing all day. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, March 21, 2012 9:35 AM To: 'Kara Lee'; dstillings@unipathdx.com; Beckham, Sharon; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank I guess everyone's idea of 'fun' can be different. A lot of these things sound like a lot of work to 'clean up'. Funny is different than making a mess to me--just 1 cent worth :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 11:19 AM To: dstillings@unipathdx.com; Beckham, Sharon; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Nope. a fun work environment breeds better workers. > From: DStillings@unipathdx.com > To: SLB@stowers.org; karabou76@hotmail.com; sbreeden@nmda.nmsu.edu; > tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > Date: Wed, 21 Mar 2012 16:17:23 +0000 > > I second that. I hope you are kidding! Hm. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Beckham, Sharon > Sent: Wednesday, March 21, 2012 9:13 AM > To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; > histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > Please tell me you are kidding about all this stuff!!!!! > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara > Lee > Sent: Wednesday, March 21, 2012 10:43 AM > To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; > histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > > While I'm new at histology, I am a master of pranks. > Here's a list of some fun ones I've done to co-workers over that > years...that haven't gotten me fired or killed yet ;) > > 1. fill their desk space with packing peanuts > > 2. If you have the time, post-it note their entire office, office > wall, ceiling and desk with the multi colored post-it notes...it's > pretty and funny > > 3. Find a dead keyboard in the IT department. Take it home, fill the > areas around the keys with dirt and chia-pet seeds. Wait for them to > sprout. Come in early in the morning and replace someones keyboard > with this one (don't actually plug it into the computer) > > 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. > > 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. > > 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. > > 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. > > Enjoy! > Kara Lee > > > > Date: Wed, 21 Mar 2012 09:27:11 -0600 > > From: sbreeden@nmda.nmsu.edu > > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > > Subject: RE: [Histonet] April fool's prank > > CC: histonet@lists.utsouthwestern.edu > > > > I always liked the M*A*S*H* one with the charcoal (?) on the > > 'scope's eyepieces. Vaseline on the coffee cup handles (don't all > > pathologists consume Mass Quantities of the stuff???). A tray of > > completely blank slides, all properly numbered for the day's work? > > > > Feed me some good ones, too. I'm down to my last week and I need to > > be remembered when I'm gone so they won't call me to do p.r.n.! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Mar 22 10:54:06 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Mar 22 10:54:47 2012 Subject: [Histonet] Nocardia procedure Message-ID: Hi all, I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 Jeanine.bartlett@cdc.hhs.gov From karabou76 <@t> hotmail.com Thu Mar 22 10:58:49 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Thu Mar 22 10:58:53 2012 Subject: [Histonet] re: april fools prank In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> Message-ID: One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From canyon <@t> path-tec.com Thu Mar 22 10:57:56 2012 From: canyon <@t> path-tec.com (Canyon Bowie) Date: Thu Mar 22 10:59:10 2012 Subject: [Histonet] Paraffin Block Shipping Question In-Reply-To: <1330101772.18432.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <1330101772.18432.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: <97F50DC5BE28F24EA6C486D8DC0729F001627EAB20C0@ORD1MBX03.mex04.mlsrvr.com> FYI www.path-tec.com has paraffin block shipping kits with ice packs if needed. Feel free to let me know if you need more information. Thanks, Canyon Bowie Path-Tec 706.507.1575 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 24, 2012 11:43 AM To: histonet@lists.utsouthwestern.edu; E V Subject: Re: [Histonet] Paraffin Block Shipping Question I used to send the overnight in one of those Styrofoam containers along with those?freeze and reuse plastic pouches inside. Ren? J. --- On Thu, 2/23/12, E V wrote: From: E V Subject: [Histonet] Paraffin Block Shipping Question To: histonet@lists.utsouthwestern.edu Date: Thursday, February 23, 2012, 4:07 PM Hi, I am wanting to send out blocks to different sites across the country. My main concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when considering the above stated concern? Thanks, H _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sun <@t> pathology.ufl.edu Thu Mar 22 11:16:16 2012 From: sun <@t> pathology.ufl.edu (Sun,Yuping) Date: Thu Mar 22 11:16:46 2012 Subject: [Histonet] re: april fools prank In-Reply-To: References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> Message-ID: <7B8BEAED567EDF4F95404483D360EFA91B997353@AHC-MB04.ad.ufl.edu> I agree. Enjoy life with your co-worker friends, and laugh! We do spend over 50% of our lives with these people. Cathy Sun Biological Scientist Molecular Pathology Core Dept. of Pathology University of Florida Room: D11-39 Lab Phone: 352-273-7754 Website: http://www.pathology.ufl.edu/~molecular/index.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMP <@t> stowers.org Thu Mar 22 11:27:10 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Thu Mar 22 11:27:19 2012 Subject: [Histonet] April fool's prank In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE974@MCINFRWEM003.ucsfmedicalcenter.org> References: , , <02C099024072804EA34F5906BAC30A413F1FF7@nmdamailsvr.nmda.ad.nmsu.edu>, , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF2@EXCHMB-02.stowers-institute.org>, <756DBA97C5CB8A409AC032D519094BEE09591F@exchange-mbx.unipathllc.corp> <2C40E43D1F7A56408C4463FD245DDDF997F37206@EXCHMB-02.stowers-institute.org> <8D7C2D242DBD45498006B21122072BF89F5EE974@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF997F3720A@EXCHMB-02.stowers-institute.org> Now that's a GREAT April Fool's Day joke--thanks for sharing it :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, March 22, 2012 10:26 AM To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Some people played a May 5 - Cinco de Mayo joke on one of our staff of Mexican heritage. He always came in early to embed and on May 5 one year they set up a cassette recorder on a timer to start playing mariachi music at 5:30 am. Then several of them came into the lab with the fake mustaches, big sombero's, woven ponchos and shakers and singing along with the music. The guy was laughing so hard he couldn't stand up. In fact he kept laughing all day. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, March 21, 2012 9:35 AM To: 'Kara Lee'; dstillings@unipathdx.com; Beckham, Sharon; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank I guess everyone's idea of 'fun' can be different. A lot of these things sound like a lot of work to 'clean up'. Funny is different than making a mess to me--just 1 cent worth :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 11:19 AM To: dstillings@unipathdx.com; Beckham, Sharon; sbreeden@nmda.nmsu.edu; tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Nope. a fun work environment breeds better workers. > From: DStillings@unipathdx.com > To: SLB@stowers.org; karabou76@hotmail.com; sbreeden@nmda.nmsu.edu; > tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > Date: Wed, 21 Mar 2012 16:17:23 +0000 > > I second that. I hope you are kidding! Hm. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Beckham, Sharon > Sent: Wednesday, March 21, 2012 9:13 AM > To: 'Kara Lee'; sbreeden@nmda.nmsu.edu; tajibade@echd.org; > histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > Please tell me you are kidding about all this stuff!!!!! > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara > Lee > Sent: Wednesday, March 21, 2012 10:43 AM > To: sbreeden@nmda.nmsu.edu; tajibade@echd.org; > histonet-bounces@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] April fool's prank > > > While I'm new at histology, I am a master of pranks. > Here's a list of some fun ones I've done to co-workers over that > years...that haven't gotten me fired or killed yet ;) > > 1. fill their desk space with packing peanuts > > 2. If you have the time, post-it note their entire office, office > wall, ceiling and desk with the multi colored post-it notes...it's > pretty and funny > > 3. Find a dead keyboard in the IT department. Take it home, fill the > areas around the keys with dirt and chia-pet seeds. Wait for them to > sprout. Come in early in the morning and replace someones keyboard > with this one (don't actually plug it into the computer) > > 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. > > 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. > > 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. > > 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. > > Enjoy! > Kara Lee > > > > Date: Wed, 21 Mar 2012 09:27:11 -0600 > > From: sbreeden@nmda.nmsu.edu > > To: tajibade@echd.org; histonet-bounces@lists.utsouthwestern.edu > > Subject: RE: [Histonet] April fool's prank > > CC: histonet@lists.utsouthwestern.edu > > > > I always liked the M*A*S*H* one with the charcoal (?) on the > > 'scope's eyepieces. Vaseline on the coffee cup handles (don't all > > pathologists consume Mass Quantities of the stuff???). A tray of > > completely blank slides, all properly numbered for the day's work? > > > > Feed me some good ones, too. I'm down to my last week and I need to > > be remembered when I'm gone so they won't call me to do p.r.n.! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Mar 22 12:34:14 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Mar 22 12:34:23 2012 Subject: [Histonet] RE: Nocardia sp in FFPE Message-ID: <001101cd0852$01a196c0$04e4c440$@bresnan.net> Jeanine, I always recall what the third edition AFIP manual (green) had to say about staining Nocardia successfully. Unfortunately, I didn't see an antibody for Nocardia. Several stains work and maybe have to do a combination of stains correlating results using adjacent sections? First choice would be GMS but you have to do some extra times in silver solution according to AFIP manual/Luna. In order to get complete development of filaments if different types/strains are suspected, run two slides with one at 60 minutes and the other at 90 minutes in the silver staining solution. Be sure to use chromic acid as the oxidizer. Churukian Shenk had a modified GMS that is supposed to work too, but nothing was said about extended times in methenamine silver solution. Fite's Rojas-Darby-Nochomovitz Method for Norcardia, TB and Leprae. This is similar to Fites but uses a Ziehl Neelsen carbol fuchsin modified with mineral oil (from Luna's Histopathological Methods and Color Atlas book) Luna Parker Method for Nocardia Asteroides (Luna book) with filaments appearing red. Brown and Brenn should show the organism too. Good luck Gayle M. Callis HTL/HT/MT(ASCP) You Wrote: I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 Jeanine.bartlett <@t> cdc.hhs.gov From Lisa.White3 <@t> va.gov Thu Mar 22 13:15:36 2012 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Mar 22 13:16:10 2012 Subject: Subject: [Histonet] Paraffin Block Shipping Question Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976097677AD@VHAV09MSGA3.v09.med.va.gov> EXAKT Technologies: Cold D-Pak for transport stock# MD8702V06; Frozen d-Pak for transport stock # MD8703V06 With current regulations on labeling and shipping of diagnostic material if you need to ship on ice or dry ice the above company has pre-labeled containers with all the federal requirements, etc as well as all the required packaging for the specimen and documents. We have to take training every year and it was explained to us that if something leaks all the powers that be out there go after the person who has their name on the shipping label (also whoever packs it has to be the one to do the label with their name). Blocks with a cold pack is a good idea during warm days. After such a mild winter I bet summer will be interesting. Also recently I was trying to find something to put a block in to mail and I had been putting them in a side loaded 5 slide container but could only get one in each one (but they cost $$$). I had been coverslipping and looked down at the empty coverslip container, VIOLA! Take the packaging out and you can get 2-3 blocks in there and they won't get squashed in the shipment and the container is free. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From tpodawiltz <@t> lrgh.org Thu Mar 22 13:20:26 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Mar 22 13:20:36 2012 Subject: [Histonet] re: April fools prank In-Reply-To: References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From robinsoc <@t> mercyhealth.com Thu Mar 22 13:24:09 2012 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Mar 22 13:24:18 2012 Subject: [Histonet] Fungus control Message-ID: <4F6B27F9020000AF000082CC@nodcdmg2.no.trinity-health.org> All, We have a lung case that is loaded with fungus. Is anyone in need? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com From dmnelson <@t> iastate.edu Thu Mar 22 13:31:50 2012 From: dmnelson <@t> iastate.edu (Gerjets, Diane M [V PTH]) Date: Thu Mar 22 13:31:55 2012 Subject: [Histonet] softening cartilage Message-ID: <56A357DCB599A04EAFC9C954B0A33DC20159DE39@ITSDAG2D.its.iastate.edu> I'm sorry if this topic has already been discussed, I don't get on the histonet very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane From thernandez709 <@t> yahoo.com Thu Mar 22 13:32:52 2012 From: thernandez709 <@t> yahoo.com (=?utf-8?B?dGhlcm5hbmRlejcwOUB5YWhvby5jb20=?=) Date: Thu Mar 22 13:32:56 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gRnVuZ3VzIGNvbnRyb2w=?= Message-ID: <860854.13965.bm@smtp110.mail.bf1.yahoo.com> Yes,we could definitely use some fungus. How can we arrange to get it ? Sent from my HTC smartphone on the Now Network from Sprint! ----- Reply message ----- From: "Cynthia Robinson" To: "histonet" Subject: [Histonet] Fungus control Date: Thu, Mar 22, 2012 1:24 pm All, We have a lung case that is loaded with fungus. Is anyone in need? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu Mar 22 13:37:38 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Mar 22 13:37:44 2012 Subject: [Histonet] Retention Standards Message-ID: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle From billodonnell <@t> catholichealth.net Thu Mar 22 13:40:19 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 22 13:40:27 2012 Subject: [Histonet] re: April fools prank In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93954D54D4@chimsx08.CHI.catholichealth.net> Tom, Not always a simple, fun prank, but bordering on the, I don't know......psycopathic? (I won't mention them, as someone might actually do them, then I'd have all those court appearences to deal with and phycological evaluations.... I just don't have that kind of time anymore) Of all the stunts we pulled, the ONLY one that ever got me in trouble was putting carbonated water in someones waterbath. Certainly one of the more benign efforts. I will likely do a lot of time in purgatory for some of those, mostly because they still cause me to smile, even after all these years. And to think we have become respected professionals and pillars of society. Who'd-a-thunk it? - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 22, 2012 1:20 PM To: Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From b-frederick <@t> northwestern.edu Thu Mar 22 13:43:57 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 22 13:44:04 2012 Subject: [Histonet] Retention Standards In-Reply-To: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> References: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsletter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Mar 22 13:46:41 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Mar 22 13:46:47 2012 Subject: [Histonet] RE: softening cartilage In-Reply-To: <56A357DCB599A04EAFC9C954B0A33DC20159DE39@ITSDAG2D.its.iastate.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5A09@SBS2K8.premierlab.local> Diane You should not need to soften cartilage, we section cartilage from many species without any additional steps to routine processing. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gerjets, Diane M [V PTH] Sent: Thursday, March 22, 2012 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] softening cartilage I'm sorry if this topic has already been discussed, I don't get on the histonet very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu Mar 22 13:46:36 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Mar 22 13:46:50 2012 Subject: [Histonet] Retention Standards In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> References: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> Message-ID: <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 From histotech <@t> imagesbyhopper.com Thu Mar 22 14:22:48 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Mar 22 14:22:54 2012 Subject: [Histonet] Retention Standards In-Reply-To: <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> References: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> Message-ID: <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com> Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 From histocontrols <@t> yahoo.com Thu Mar 22 14:35:49 2012 From: histocontrols <@t> yahoo.com (Rich Yeo) Date: Thu Mar 22 14:35:56 2012 Subject: [Histonet] Retention Standards In-Reply-To: <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com> References: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com> Message-ID: <1332444949.74857.YahooMailNeo@web140417.mail.bf1.yahoo.com> There is another update from Jan. 2012. It should be on thier web site R ________________________________ From: "histotech@imagesbyhopper.com" To: 'Bernice Frederick' ; histonet@lists.utsouthwestern.edu Sent: Thursday, March 22, 2012 3:22 PM Subject: RE: [Histonet] Retention Standards Here is an updated CAP compendium retention guidelines list.? This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one!? That's one down.? :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines?? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines.? I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu Mar 22 15:50:24 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Mar 22 15:50:36 2012 Subject: [Histonet] Retention Standards In-Reply-To: <1332444949.74857.YahooMailNeo@web140417.mail.bf1.yahoo.com> References: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com> <1332444949.74857.YahooMailNeo@web140417.mail.bf1.yahoo.com> Message-ID: <000c01cd086d$6bac16b0$43044410$@imagesbyhopper.com> I just got off the phone with CAP and the 2010 revision is their most recent. :o) I also found the CLIA retention guidelines. they can be found at the following URL: https://www.cms.gov/CLIA/03_Interpretive_Guidelines_for_Laboratories.asp 2 down, 2 to go! Michelle From: Rich Yeo [mailto:histocontrols@yahoo.com] Sent: Thursday, March 22, 2012 3:36 PM To: histotech@imagesbyhopper.com; 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Retention Standards There is another update from Jan. 2012. It should be on thier web site R _____ From: "histotech@imagesbyhopper.com" To: 'Bernice Frederick' ; histonet@lists.utsouthwestern.edu Sent: Thursday, March 22, 2012 3:22 PM Subject: RE: [Histonet] Retention Standards Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true &cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true &cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Mar 22 16:09:52 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Mar 22 16:10:00 2012 Subject: [Histonet] Fixatives for breast In-Reply-To: References: Message-ID: <4F6B5CE0.7400.0077.1@harthosp.org> 1.) Formalin, 2.) Formalin, and 3.) Formalin. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> LaSalette Charette 3/21/2012 12:59 PM >>> What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Thu Mar 22 18:23:54 2012 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Thu Mar 22 18:25:04 2012 Subject: [Histonet] Nocardia procedure In-Reply-To: References: Message-ID: <4F6C4129.411C.0039.0@health.qld.gov.au> Hi Jeanine I have always just used a Fite and it has worked well. If you use a Gram you will find that they are Gram variable in their staining. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Bartlett, Jeanine (CDC/OID/NCEZID)" 3/23/2012 1:54 am >>> Hi all, I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 Jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From katelin09htl <@t> gmail.com Thu Mar 22 18:42:49 2012 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Thu Mar 22 18:42:55 2012 Subject: [Histonet] Part Time Histotech with Benefits near Portland, OR Message-ID: Part time certified HT or HTL wanted for busy GI practice in Tualatin, Oregon. Candidate must be ASCP certified and meet CLIA requirements to perform gross dissection. GI experience preferred. This is a M-F position with no weekends , holidays or call. Includes Full Medical & Dental benefits plus a 401K program. Interested applicants should forward their resume to gbacon@gispecialists.org From marccentofante <@t> gmail.com Fri Mar 23 06:54:07 2012 From: marccentofante <@t> gmail.com (marc centofante) Date: Fri Mar 23 06:54:14 2012 Subject: [Histonet] Post question Message-ID: I am not sure how to post to histonet but if I email this address and then it gets posted here is what I would like to post: My employer is looking to buy an anatomic pathology lab with frozen section service. Does anyone know of any labs for sale? -- Thank you, Marc Centofante marccentofante@gmail.com From sonya.martin <@t> soton.ac.uk Fri Mar 23 07:25:27 2012 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Fri Mar 23 07:25:46 2012 Subject: [Histonet] Problems using tomato lectin Message-ID: <5BE0438A6D85E645A67A4A2F58F41693F639@UOS-MSG00041-SI.soton.ac.uk> Hi all, I've just purchased Texas-Red conjugated tomato lectin from Vector and have been trying it out on some frozen mouse liver sections but cant see anything. Protocol - fresh frozen sections 8um cut and air dried overnight. Fixed (10min) acetone, washed PBS, tomato lectin applied (I've done serial dilutions from 1/100 - 1/5000 in PBS/0.05% Tween) 1hr at room temp, wash PBS, mount Vectashield + dapi. Any suggestions? Thanks Sonya From sonya.martin <@t> soton.ac.uk Fri Mar 23 07:34:01 2012 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Fri Mar 23 07:34:48 2012 Subject: [Histonet] Mouse abs on mouse tissue method Message-ID: <5BE0438A6D85E645A67A4A2F58F41693F64A@UOS-MSG00041-SI.soton.ac.uk> I've been trying out the method from IHC world to use mouse primary plus anti-mouse secondary abs on mouse tissue. Protocol; Fresh frozen sections (mouse spleen) cut 8um and air dried Fixed acetone 10min Wash PBS Block with 2.5% normal goat serum in PBS/Tw, 30min Block with unconjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, diluted 1/10 in PBS/Tw), 2hrs room temp Wash PBS/Tw Seconary ab - DyLight488 conjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, 1/500 PBS/Tw), 20min room temp Wash PBS/Tw Mount Blocking with unconjugated Fab did decrease background if I used an IgG goat anti-mouse secondary but the background was actually increased when I used the Fab goat anti-mouse!! Any suggestions? Thanks Sonya From badzrosari <@t> yahoo.com Fri Mar 23 07:42:33 2012 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Fri Mar 23 07:42:42 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES Message-ID: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Good day histonetters.We are a ?new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern? which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me??? From rjbuesa <@t> yahoo.com Fri Mar 23 08:10:31 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 23 08:10:39 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: <1332508231.59558.YahooMailClassic@web162103.mail.bf1.yahoo.com> There is no standard for color cassettes. That?will be?your choice. I used the following: Green for "rush" specimens. Yellow for skins. Pink for bone marrows. Blue for autopsy specimens. Violet for gastric biopsies. White for routine specimens. Prepare something like this and submit it to your pathologist for approval. Ren? J. --- On Fri, 3/23/12, Bernadette del Rosario wrote: From: Bernadette del Rosario Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES To: "histonet@lists.utsouthwestern.edu" Date: Friday, March 23, 2012, 8:42 AM Good day histonetters.We are a ?new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern? which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Fri Mar 23 08:13:44 2012 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Fri Mar 23 08:14:29 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397609767A36@VHAV09MSGA3.v09.med.va.gov> I am not aware of a standardized way to color code cassettes. I have worked at several labs and it is individualized to each lab needs. Currently we have red for stat or special stain, white for large specimens, blue for biopsies, green for autopsy, yellow for bone, pink for cell blocks, orange for fna cell blocks, we have 4 colors for prostate biopsies gray, aqua, lilac, & peach with color coordinated slides to be able to make sure no biopsies make it into the wrong case (we run 4 cases per overnight processing run). Just make an inventory of all your tissues and decide on the color scheme that would suit you best. Ours helps to speed things up in the morning when embedding so that you can find the stat items first. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From talulahgosh <@t> gmail.com Fri Mar 23 08:29:59 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Mar 23 08:30:07 2012 Subject: [Histonet] Mouse abs on mouse tissue method In-Reply-To: <5BE0438A6D85E645A67A4A2F58F41693F64A@UOS-MSG00041-SI.soton.ac.uk> References: <5BE0438A6D85E645A67A4A2F58F41693F64A@UOS-MSG00041-SI.soton.ac.uk> Message-ID: This doesn't really help solve your problem, but I have a question. Why are you using two secondary antibodies? Why not just use the conjugated one? Emily (starting Friday off right by eating candy for breakfast. I love Fridays!!) The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Fri, Mar 23, 2012 at 8:34 AM, James S. wrote: > I've been trying out the method from IHC world to use mouse primary plus > anti-mouse secondary abs on mouse tissue. > > Protocol; > > Fresh frozen sections (mouse spleen) cut 8um and air dried > Fixed acetone 10min > Wash PBS > Block with 2.5% normal goat serum in PBS/Tw, 30min > Block with unconjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, > diluted 1/10 in PBS/Tw), 2hrs room temp > Wash PBS/Tw > Seconary ab - DyLight488 conjugated Fab fragment goat anti-mouse IgG > (Jackson Immuno, 1/500 PBS/Tw), 20min room temp > Wash PBS/Tw > Mount > > Blocking with unconjugated Fab did decrease background if I used an IgG > goat anti-mouse secondary but the background was actually increased when I > used the Fab goat anti-mouse!! > > Any suggestions? > > Thanks > Sonya > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Carol.Freeman <@t> utoledo.edu Fri Mar 23 08:37:36 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Fri Mar 23 08:38:05 2012 Subject: [Histonet] Tissue Micro Array Devices In-Reply-To: <32c97bf3-b654-4037-9d50-47cc36f1534a@MsgApp10.utad.utoledo.edu> References: <32c97bf3-b654-4037-9d50-47cc36f1534a@MsgApp10.utad.utoledo.edu> Message-ID: Good Morning and Happy Friday fellow histonetters :) I am looking into getting a Tissue Micro Array Device to make some nice multi-tissue (normal) and multi-tumor blocks for controls, validations and lot checks for IHC. Has anyone any advice for purchasing one, any favorites or any we should stay from....Any responses are appreciated. Thanks in advance ;) Carol Freeman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, March 23, 2012 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 100, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Nocardia sp in FFPE (gayle callis) 2. Subject: [Histonet] Paraffin Block Shipping Question (White, Lisa M.) 3. RE: re: April fools prank (Podawiltz, Thomas) 4. Fungus control (Cynthia Robinson) 5. softening cartilage (Gerjets, Diane M [V PTH]) 6. Re: Fungus control ( thernandez709@yahoo.com ) 7. Retention Standards (histotech@imagesbyhopper.com) 8. RE: re: April fools prank (O'Donnell, Bill) 9. RE: Retention Standards (Bernice Frederick) 10. RE: softening cartilage (Elizabeth Chlipala) 11. RE: Retention Standards (histotech@imagesbyhopper.com) 12. RE: Retention Standards (histotech@imagesbyhopper.com) 13. Re: Retention Standards (Rich Yeo) 14. RE: Retention Standards (histotech@imagesbyhopper.com) 15. Re: Fixatives for breast (Richard Cartun) 16. Re: Nocardia procedure (Tony Reilly) 17. Part Time Histotech with Benefits near Portland, OR (Katelin Lester) 18. Post question (marc centofante) 19. Problems using tomato lectin (James S.) 20. Mouse abs on mouse tissue method (James S.) 21. PROTOCOL FOR COLOR CODING BIOPSY CASSETTES (Bernadette del Rosario) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 Mar 2012 11:34:14 -0600 From: "gayle callis" Subject: [Histonet] RE: Nocardia sp in FFPE To: "Histonet" Message-ID: <001101cd0852$01a196c0$04e4c440$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Jeanine, I always recall what the third edition AFIP manual (green) had to say about staining Nocardia successfully. Unfortunately, I didn't see an antibody for Nocardia. Several stains work and maybe have to do a combination of stains correlating results using adjacent sections? First choice would be GMS but you have to do some extra times in silver solution according to AFIP manual/Luna. In order to get complete development of filaments if different types/strains are suspected, run two slides with one at 60 minutes and the other at 90 minutes in the silver staining solution. Be sure to use chromic acid as the oxidizer. Churukian Shenk had a modified GMS that is supposed to work too, but nothing was said about extended times in methenamine silver solution. Fite's Rojas-Darby-Nochomovitz Method for Norcardia, TB and Leprae. This is similar to Fites but uses a Ziehl Neelsen carbol fuchsin modified with mineral oil (from Luna's Histopathological Methods and Color Atlas book) Luna Parker Method for Nocardia Asteroides (Luna book) with filaments appearing red. Brown and Brenn should show the organism too. Good luck Gayle M. Callis HTL/HT/MT(ASCP) You Wrote: I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 Jeanine.bartlett <@t> cdc.hhs.gov ------------------------------ Message: 2 Date: Thu, 22 Mar 2012 14:15:36 -0400 From: "White, Lisa M." Subject: Subject: [Histonet] Paraffin Block Shipping Question To: Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976097677AD@VHAV09MSGA3.v09.med.va.gov> Content-Type: text/plain; charset="us-ascii" EXAKT Technologies: Cold D-Pak for transport stock# MD8702V06; Frozen d-Pak for transport stock # MD8703V06 With current regulations on labeling and shipping of diagnostic material if you need to ship on ice or dry ice the above company has pre-labeled containers with all the federal requirements, etc as well as all the required packaging for the specimen and documents. We have to take training every year and it was explained to us that if something leaks all the powers that be out there go after the person who has their name on the shipping label (also whoever packs it has to be the one to do the label with their name). Blocks with a cold pack is a good idea during warm days. After such a mild winter I bet summer will be interesting. Also recently I was trying to find something to put a block in to mail and I had been putting them in a side loaded 5 slide container but could only get one in each one (but they cost $$$). I had been coverslipping and looked down at the empty coverslip container, VIOLA! Take the packaging out and you can get 2-3 blocks in there and they won't get squashed in the shipment and the container is free. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ------------------------------ Message: 3 Date: Thu, 22 Mar 2012 14:20:26 -0400 From: "Podawiltz, Thomas" Subject: RE: [Histonet] re: April fools prank To: Kara Lee , "histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 4 Date: Thu, 22 Mar 2012 14:24:09 -0400 From: "Cynthia Robinson" Subject: [Histonet] Fungus control To: "histonet" Message-ID: <4F6B27F9020000AF000082CC@nodcdmg2.no.trinity-health.org> Content-Type: text/plain; charset=US-ASCII All, We have a lung case that is loaded with fungus. Is anyone in need? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com ------------------------------ Message: 5 Date: Thu, 22 Mar 2012 18:31:50 +0000 From: "Gerjets, Diane M [V PTH]" Subject: [Histonet] softening cartilage To: "histonet@lists.utsouthwestern.edu" Message-ID: <56A357DCB599A04EAFC9C954B0A33DC20159DE39@ITSDAG2D.its.iastate.edu> Content-Type: text/plain; charset="us-ascii" I'm sorry if this topic has already been discussed, I don't get on the histonet very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane ------------------------------ Message: 6 Date: Thu, 22 Mar 2012 13:32:52 -0500 From: " thernandez709@yahoo.com " Subject: Re: [Histonet] Fungus control To: robinsoc@mercyhealth.com,"histonet" Message-ID: <860854.13965.bm@smtp110.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 Yes,we could definitely use some fungus. How can we arrange to get it ? Sent from my HTC smartphone on the Now Network from Sprint! ----- Reply message ----- From: "Cynthia Robinson" To: "histonet" Subject: [Histonet] Fungus control Date: Thu, Mar 22, 2012 1:24 pm All, We have a lung case that is loaded with fungus. Is anyone in need? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 22 Mar 2012 14:37:38 -0400 From: Subject: [Histonet] Retention Standards To: Message-ID: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com> Content-Type: text/plain; charset=US-ASCII Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle ------------------------------ Message: 8 Date: Thu, 22 Mar 2012 12:40:19 -0600 From: "O'Donnell, Bill" Subject: RE: [Histonet] re: April fools prank To: "Podawiltz, Thomas" , "Kara Lee" , Message-ID: <4940DF6D1C5FDF48931B6966AAEF93954D54D4@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" Tom, Not always a simple, fun prank, but bordering on the, I don't know......psycopathic? (I won't mention them, as someone might actually do them, then I'd have all those court appearences to deal with and phycological evaluations.... I just don't have that kind of time anymore) Of all the stunts we pulled, the ONLY one that ever got me in trouble was putting carbonated water in someones waterbath. Certainly one of the more benign efforts. I will likely do a lot of time in purgatory for some of those, mostly because they still cause me to smile, even after all these years. And to think we have become respected professionals and pillars of society. Who'd-a-thunk it? - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 22, 2012 1:20 PM To: Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 9 Date: Thu, 22 Mar 2012 18:43:57 +0000 From: Bernice Frederick Subject: RE: [Histonet] Retention Standards To: "histotech@imagesbyhopper.com" , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E227469@CHCSPMBX4.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsletter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 22 Mar 2012 12:46:41 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: softening cartilage To: "'Gerjets, Diane M [V PTH]'" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5A09@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Diane You should not need to soften cartilage, we section cartilage from many species without any additional steps to routine processing. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gerjets, Diane M [V PTH] Sent: Thursday, March 22, 2012 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] softening cartilage I'm sorry if this topic has already been discussed, I don't get on the histonet very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 22 Mar 2012 14:46:36 -0400 From: Subject: RE: [Histonet] Retention Standards To: "'Bernice Frederick'" , Message-ID: <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com> Content-Type: text/plain; charset=US-ASCII Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 ------------------------------ Message: 12 Date: Thu, 22 Mar 2012 15:22:48 -0400 From: Subject: RE: [Histonet] Retention Standards To: "'Bernice Frederick'" , Message-ID: <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com> Content-Type: text/plain; charset=US-ASCII Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 ------------------------------ Message: 13 Date: Thu, 22 Mar 2012 12:35:49 -0700 (PDT) From: Rich Yeo Subject: Re: [Histonet] Retention Standards To: "histotech@imagesbyhopper.com" , 'Bernice Frederick' , "histonet@lists.utsouthwestern.edu" Message-ID: <1332444949.74857.YahooMailNeo@web140417.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There is another update from Jan. 2012. It should be on thier web site R ________________________________ From: "histotech@imagesbyhopper.com" To: 'Bernice Frederick' ; histonet@lists.utsouthwestern.edu Sent: Thursday, March 22, 2012 3:22 PM Subject: RE: [Histonet] Retention Standards Here is an updated CAP compendium retention guidelines list.? This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one!? That's one down.? :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines?? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines.? I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 22 Mar 2012 16:50:24 -0400 From: Subject: RE: [Histonet] Retention Standards To: "'Rich Yeo'" , "'Bernice Frederick'" , Message-ID: <000c01cd086d$6bac16b0$43044410$@imagesbyhopper.com> Content-Type: text/plain; charset="us-ascii" I just got off the phone with CAP and the 2010 revision is their most recent. :o) I also found the CLIA retention guidelines. they can be found at the following URL: https://www.cms.gov/CLIA/03_Interpretive_Guidelines_for_Laboratories.asp 2 down, 2 to go! Michelle From: Rich Yeo [mailto:histocontrols@yahoo.com] Sent: Thursday, March 22, 2012 3:36 PM To: histotech@imagesbyhopper.com; 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Retention Standards There is another update from Jan. 2012. It should be on thier web site R _____ From: "histotech@imagesbyhopper.com" To: 'Bernice Frederick' ; histonet@lists.utsouthwestern.edu Sent: Thursday, March 22, 2012 3:22 PM Subject: RE: [Histonet] Retention Standards Here is an updated CAP compendium retention guidelines list. This was last updated in March, 2010 http://www.cap.org/apps/cap.portal?_nfpb=true &cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa bel=cntvwr Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 2:47 PM To: 'Bernice Frederick'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards Bernice, Thanks for that one! That's one down. :o) JC anyone? Florida?? :) Michelle -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 22, 2012 2:44 PM To: histotech@imagesbyhopper.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retention Standards You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=true &cntvwrPtlt_actionOverride=%2Fp ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle tter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 22 Mar 2012 17:09:52 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Fixatives for breast To: "LaSalette Charette" , "histonet@lists.utsouthwestern.edu" Message-ID: <4F6B5CE0.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII 1.) Formalin, 2.) Formalin, and 3.) Formalin. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> LaSalette Charette 3/21/2012 12:59 PM >>> What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 23 Mar 2012 09:23:54 +1000 From: "Tony Reilly" Subject: Re: [Histonet] Nocardia procedure To: "Jeanine (CDC/OID/NCEZID) Bartlett" , "histonet@lists.utsouthwestern.edu" Message-ID: <4F6C4129.411C.0039.0@health.qld.gov.au> Content-Type: text/plain; charset="us-ascii" Hi Jeanine I have always just used a Fite and it has worked well. If you use a Gram you will find that they are Gram variable in their staining. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Bartlett, Jeanine (CDC/OID/NCEZID)" 3/23/2012 1:54 am >>> Hi all, I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue. Thanks in advance! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 Jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 17 Date: Thu, 22 Mar 2012 16:42:49 -0700 From: Katelin Lester Subject: [Histonet] Part Time Histotech with Benefits near Portland, OR To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Part time certified HT or HTL wanted for busy GI practice in Tualatin, Oregon. Candidate must be ASCP certified and meet CLIA requirements to perform gross dissection. GI experience preferred. This is a M-F position with no weekends , holidays or call. Includes Full Medical & Dental benefits plus a 401K program. Interested applicants should forward their resume to gbacon@gispecialists.org ------------------------------ Message: 18 Date: Fri, 23 Mar 2012 07:54:07 -0400 From: marc centofante Subject: [Histonet] Post question To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I am not sure how to post to histonet but if I email this address and then it gets posted here is what I would like to post: My employer is looking to buy an anatomic pathology lab with frozen section service. Does anyone know of any labs for sale? -- Thank you, Marc Centofante marccentofante@gmail.com ------------------------------ Message: 19 Date: Fri, 23 Mar 2012 12:25:27 +0000 From: "James S." Subject: [Histonet] Problems using tomato lectin To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <5BE0438A6D85E645A67A4A2F58F41693F639@UOS-MSG00041-SI.soton.ac.uk> Content-Type: text/plain; charset="us-ascii" Hi all, I've just purchased Texas-Red conjugated tomato lectin from Vector and have been trying it out on some frozen mouse liver sections but cant see anything. Protocol - fresh frozen sections 8um cut and air dried overnight. Fixed (10min) acetone, washed PBS, tomato lectin applied (I've done serial dilutions from 1/100 - 1/5000 in PBS/0.05% Tween) 1hr at room temp, wash PBS, mount Vectashield + dapi. Any suggestions? Thanks Sonya ------------------------------ Message: 20 Date: Fri, 23 Mar 2012 12:34:01 +0000 From: "James S." Subject: [Histonet] Mouse abs on mouse tissue method To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <5BE0438A6D85E645A67A4A2F58F41693F64A@UOS-MSG00041-SI.soton.ac.uk> Content-Type: text/plain; charset="us-ascii" I've been trying out the method from IHC world to use mouse primary plus anti-mouse secondary abs on mouse tissue. Protocol; Fresh frozen sections (mouse spleen) cut 8um and air dried Fixed acetone 10min Wash PBS Block with 2.5% normal goat serum in PBS/Tw, 30min Block with unconjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, diluted 1/10 in PBS/Tw), 2hrs room temp Wash PBS/Tw Seconary ab - DyLight488 conjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, 1/500 PBS/Tw), 20min room temp Wash PBS/Tw Mount Blocking with unconjugated Fab did decrease background if I used an IgG goat anti-mouse secondary but the background was actually increased when I used the Fab goat anti-mouse!! Any suggestions? Thanks Sonya ------------------------------ Message: 21 Date: Fri, 23 Mar 2012 05:42:33 -0700 (PDT) From: Bernadette del Rosario Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES To: "histonet@lists.utsouthwestern.edu" Message-ID: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good day histonetters.We are a ?new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern? which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me??? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 32 ***************************************** From bakevictoria <@t> gmail.com Fri Mar 23 08:52:33 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Mar 23 08:52:41 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: Hi Bernadette, I know of several different color code set ups. Pink - breast tissue (usually has separate processing cycle for ER/PR....) Lt. Green - biopsy tissue (has separate processing cycle - short run) Tan - bone marrow (has special stains and IHC built into the protocol) Lavender - Prostate biopsies (short run also PIN4 built into protocol) Aqua - skin/derm specimens Red - "stat" or "rush" specimens" Grey - urate crystal (special processing) White - all other surgical material including breast tissue that does not require special protocols Blue - autopsy material In some labs they correspond the cassettes and the slides (green cassette = green slide). In this context you will look at having both regular (superfrost) and plus slides available as well. There really isn't a set protocol it is more a way of easy identification for processing and also which case/specimen take priority. Faster identification for loading of processors (breast tissue, biopsy, priority cases) ,embedding (priority and embedding orientation), cutting (special stains, add'l sections etc). I'm not fond of the red cassettes because they are difficult to read if not imprinted properly, but that color stood out best and most techs associated it with 'move it out fast'. I did get a chuckle when a tech once asked me why we had lavender for prostate biopsies and I had to tell him that the blue had already been taken ;-). One other thing that I had consternation about was putting fatty breast tissue from a reduction in white because of grossing/processing issues. I could not get assistance through the Doc's for this so there were re-pro's until I was able to get ALL breast tissue put on the longer processing cycle. It didn't make me a lot of friends in the grossing area though. For the biopsies you may want to be sure you have matching mesh cassettes. The lavender ones I usually always used mesh. Look at what your specimen types will be and associate them with something that is easily recognized by staff. There isn't a set protocol for it though. Sorry and I hope this helps. Vikki On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario < badzrosari@yahoo.com> wrote: > Good day histonetters.We are a new university hospital and setting up > histopatholology lab.I used to work with white biopsy cassettes only but > not technicolors.Got this boss who ask me protocols on colored cassettes > etc...No idea about this.Is there any standard pattern which i can just > base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in > the net but cant find..Please someone help me??? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LRaff <@t> uropartners.com Fri Mar 23 09:01:53 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Mar 23 09:02:01 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: "Lavender - Prostate biopsies (short run also PIN4 built into protocol)" No! No! NO! This is why we are running into trouble on reimbursements!!! PIN4 should NOT be done routinely. If you mean cutting a slide for potential PIN4, that's fine, but no pathologist should automatically be doing a PIN4 on every prostate biopsy Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, March 23, 2012 8:53 AM To: Bernadette del Rosario Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES Hi Bernadette, I know of several different color code set ups. Pink - breast tissue (usually has separate processing cycle for ER/PR....) Lt. Green - biopsy tissue (has separate processing cycle - short run) Tan - bone marrow (has special stains and IHC built into the protocol) Lavender - Prostate biopsies (short run also PIN4 built into protocol) Aqua - skin/derm specimens Red - "stat" or "rush" specimens" Grey - urate crystal (special processing) White - all other surgical material including breast tissue that does not require special protocols Blue - autopsy material In some labs they correspond the cassettes and the slides (green cassette = green slide). In this context you will look at having both regular (superfrost) and plus slides available as well. There really isn't a set protocol it is more a way of easy identification for processing and also which case/specimen take priority. Faster identification for loading of processors (breast tissue, biopsy, priority cases) ,embedding (priority and embedding orientation), cutting (special stains, add'l sections etc). I'm not fond of the red cassettes because they are difficult to read if not imprinted properly, but that color stood out best and most techs associated it with 'move it out fast'. I did get a chuckle when a tech once asked me why we had lavender for prostate biopsies and I had to tell him that the blue had already been taken ;-). One other thing that I had consternation about was putting fatty breast tissue from a reduction in white because of grossing/processing issues. I could not get assistance through the Doc's for this so there were re-pro's until I was able to get ALL breast tissue put on the longer processing cycle. It didn't make me a lot of friends in the grossing area though. For the biopsies you may want to be sure you have matching mesh cassettes. The lavender ones I usually always used mesh. Look at what your specimen types will be and associate them with something that is easily recognized by staff. There isn't a set protocol for it though. Sorry and I hope this helps. Vikki On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario < badzrosari@yahoo.com> wrote: > Good day histonetters.We are a new university hospital and setting up > histopatholology lab.I used to work with white biopsy cassettes only but > not technicolors.Got this boss who ask me protocols on colored cassettes > etc...No idea about this.Is there any standard pattern which i can just > base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in > the net but cant find..Please someone help me??? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Mar 23 09:07:28 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Mar 23 09:07:37 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: My Bad for not explaining fully - not all prostate biopsies do get PIN4, additional sections are cut and saved for possible future requests on prostate biopsies. This cannot be done automatically for the very reason you describe. My intention was to point out that we separated these out so that the cutters would know to cut the additional sections. My apologies for not fully explaining this. Vikki On Fri, Mar 23, 2012 at 10:01 AM, Lester Raff MD wrote: > "Lavender - Prostate biopsies (short run also PIN4 built into protocol)" > > No! No! NO! This is why we are running into trouble on > reimbursements!!! PIN4 should NOT be done routinely. If you mean > cutting a slide for potential PIN4, that's fine, but no pathologist > should automatically be doing a PIN4 on every prostate biopsy > > Lester J. Raff, MD > Medical Director > UroPartners Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel 708.486.0076 > Fax 708.492.0203 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria > Baker > Sent: Friday, March 23, 2012 8:53 AM > To: Bernadette del Rosario > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES > > Hi Bernadette, > > I know of several different color code set ups. > Pink - breast tissue (usually has separate processing cycle for > ER/PR....) > Lt. Green - biopsy tissue (has separate processing cycle - short run) > Tan - bone marrow (has special stains and IHC built into the > protocol) > Lavender - Prostate biopsies (short run also PIN4 built into protocol) > Aqua - skin/derm specimens > Red - "stat" or "rush" specimens" > Grey - urate crystal (special processing) > White - all other surgical material including breast tissue that does > not > require special protocols > Blue - autopsy material > > In some labs they correspond the cassettes and the slides (green > cassette = > green slide). In this context you will look at having both regular > (superfrost) and plus slides available as well. > > There really isn't a set protocol it is more a way of easy > identification > for processing and also which case/specimen take priority. Faster > identification for loading of processors (breast tissue, biopsy, > priority > cases) ,embedding (priority and embedding orientation), cutting (special > stains, add'l sections etc). > > I'm not fond of the red cassettes because they are difficult to read if > not > imprinted properly, but that color stood out best and most techs > associated > it with 'move it out fast'. I did get a chuckle when a tech once asked > me > why we had lavender for prostate biopsies and I had to tell him that the > blue had already been taken ;-). One other thing that I had > consternation > about was putting fatty breast tissue from a reduction in white because > of > grossing/processing issues. I could not get assistance through the > Doc's > for this so there were re-pro's until I was able to get ALL breast > tissue > put on the longer processing cycle. It didn't make me a lot of friends > in > the grossing area though. > > For the biopsies you may want to be sure you have matching mesh > cassettes. > The lavender ones I usually always used mesh. > > Look at what your specimen types will be and associate them with > something > that is easily recognized by staff. There isn't a set protocol for it > though. Sorry and I hope this helps. > > Vikki > > > > > > > > > On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario < > badzrosari@yahoo.com> wrote: > > > Good day histonetters.We are a new university hospital and setting up > > histopatholology lab.I used to work with white biopsy cassettes only > but > > not technicolors.Got this boss who ask me protocols on colored > cassettes > > etc...No idea about this.Is there any standard pattern which i can > just > > base and copy (example skin-yellow;breast-pink etc..)Im trying to surf > in > > the net but cant find..Please someone help me??? > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LStadler <@t> cbiolabs.com Fri Mar 23 09:10:07 2012 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Fri Mar 23 09:10:14 2012 Subject: [Histonet] Murine Intestine Protocols Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC725ABFA1@cbiolabs05.CBiolabs.local> Hello all ~ If anyone could share their protocols for intestine processing, embedding, cutting, etc I would greatly appreciate any input. I am having trouble getting consistently good quality intestine sections and am always looking to improve. Also, does anyone have an experience with a product called "GI FIX" from BBC/Histoperfect (Item # 1111)? Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From LRaff <@t> uropartners.com Fri Mar 23 09:10:14 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Mar 23 09:10:23 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: Thanks for the explanation! I didn't mean to jump on anyone, after all, it's Friday! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, March 23, 2012 9:07 AM To: Lester Raff MD Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES My Bad for not explaining fully - not all prostate biopsies do get PIN4, additional sections are cut and saved for possible future requests on prostate biopsies. This cannot be done automatically for the very reason you describe. My intention was to point out that we separated these out so that the cutters would know to cut the additional sections. My apologies for not fully explaining this. Vikki On Fri, Mar 23, 2012 at 10:01 AM, Lester Raff MD wrote: > "Lavender - Prostate biopsies (short run also PIN4 built into protocol)" > > No! No! NO! This is why we are running into trouble on > reimbursements!!! PIN4 should NOT be done routinely. If you mean > cutting a slide for potential PIN4, that's fine, but no pathologist > should automatically be doing a PIN4 on every prostate biopsy > > Lester J. Raff, MD > Medical Director > UroPartners Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel 708.486.0076 > Fax 708.492.0203 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria > Baker > Sent: Friday, March 23, 2012 8:53 AM > To: Bernadette del Rosario > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES > > Hi Bernadette, > > I know of several different color code set ups. > Pink - breast tissue (usually has separate processing cycle for > ER/PR....) > Lt. Green - biopsy tissue (has separate processing cycle - short run) > Tan - bone marrow (has special stains and IHC built into the > protocol) > Lavender - Prostate biopsies (short run also PIN4 built into protocol) > Aqua - skin/derm specimens > Red - "stat" or "rush" specimens" > Grey - urate crystal (special processing) > White - all other surgical material including breast tissue that does > not > require special protocols > Blue - autopsy material > > In some labs they correspond the cassettes and the slides (green > cassette = > green slide). In this context you will look at having both regular > (superfrost) and plus slides available as well. > > There really isn't a set protocol it is more a way of easy > identification > for processing and also which case/specimen take priority. Faster > identification for loading of processors (breast tissue, biopsy, > priority > cases) ,embedding (priority and embedding orientation), cutting (special > stains, add'l sections etc). > > I'm not fond of the red cassettes because they are difficult to read if > not > imprinted properly, but that color stood out best and most techs > associated > it with 'move it out fast'. I did get a chuckle when a tech once asked > me > why we had lavender for prostate biopsies and I had to tell him that the > blue had already been taken ;-). One other thing that I had > consternation > about was putting fatty breast tissue from a reduction in white because > of > grossing/processing issues. I could not get assistance through the > Doc's > for this so there were re-pro's until I was able to get ALL breast > tissue > put on the longer processing cycle. It didn't make me a lot of friends > in > the grossing area though. > > For the biopsies you may want to be sure you have matching mesh > cassettes. > The lavender ones I usually always used mesh. > > Look at what your specimen types will be and associate them with > something > that is easily recognized by staff. There isn't a set protocol for it > though. Sorry and I hope this helps. > > Vikki > > > > > > > > > On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario < > badzrosari@yahoo.com> wrote: > > > Good day histonetters.We are a new university hospital and setting up > > histopatholology lab.I used to work with white biopsy cassettes only > but > > not technicolors.Got this boss who ask me protocols on colored > cassettes > > etc...No idea about this.Is there any standard pattern which i can > just > > base and copy (example skin-yellow;breast-pink etc..)Im trying to surf > in > > the net but cant find..Please someone help me??? > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Mar 23 09:11:00 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Mar 23 09:11:09 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: No I understand fully what you are saying. Have a great weekend. On Fri, Mar 23, 2012 at 10:10 AM, Lester Raff MD wrote: > Thanks for the explanation! I didn't mean to jump on anyone, after all, > it's Friday! > > Lester J. Raff, MD > Medical Director > UroPartners Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel 708.486.0076 > Fax 708.492.0203 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria > Baker > Sent: Friday, March 23, 2012 9:07 AM > To: Lester Raff MD > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES > > My Bad for not explaining fully - not all prostate biopsies do get PIN4, > additional sections are cut and saved for possible future requests on > prostate biopsies. This cannot be done automatically for the very > reason > you describe. My intention was to point out that we separated these out > so > that the cutters would know to cut the additional sections. > > My apologies for not fully explaining this. > > Vikki > > On Fri, Mar 23, 2012 at 10:01 AM, Lester Raff MD > wrote: > > > "Lavender - Prostate biopsies (short run also PIN4 built into > protocol)" > > > > No! No! NO! This is why we are running into trouble on > > reimbursements!!! PIN4 should NOT be done routinely. If you mean > > cutting a slide for potential PIN4, that's fine, but no pathologist > > should automatically be doing a PIN4 on every prostate biopsy > > > > Lester J. Raff, MD > > Medical Director > > UroPartners Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel 708.486.0076 > > Fax 708.492.0203 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Victoria > > Baker > > Sent: Friday, March 23, 2012 8:53 AM > > To: Bernadette del Rosario > > Cc: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES > > > > Hi Bernadette, > > > > I know of several different color code set ups. > > Pink - breast tissue (usually has separate processing cycle for > > ER/PR....) > > Lt. Green - biopsy tissue (has separate processing cycle - short run) > > Tan - bone marrow (has special stains and IHC built into the > > protocol) > > Lavender - Prostate biopsies (short run also PIN4 built into protocol) > > Aqua - skin/derm specimens > > Red - "stat" or "rush" specimens" > > Grey - urate crystal (special processing) > > White - all other surgical material including breast tissue that does > > not > > require special protocols > > Blue - autopsy material > > > > In some labs they correspond the cassettes and the slides (green > > cassette = > > green slide). In this context you will look at having both regular > > (superfrost) and plus slides available as well. > > > > There really isn't a set protocol it is more a way of easy > > identification > > for processing and also which case/specimen take priority. Faster > > identification for loading of processors (breast tissue, biopsy, > > priority > > cases) ,embedding (priority and embedding orientation), cutting > (special > > stains, add'l sections etc). > > > > I'm not fond of the red cassettes because they are difficult to read > if > > not > > imprinted properly, but that color stood out best and most techs > > associated > > it with 'move it out fast'. I did get a chuckle when a tech once > asked > > me > > why we had lavender for prostate biopsies and I had to tell him that > the > > blue had already been taken ;-). One other thing that I had > > consternation > > about was putting fatty breast tissue from a reduction in white > because > > of > > grossing/processing issues. I could not get assistance through the > > Doc's > > for this so there were re-pro's until I was able to get ALL breast > > tissue > > put on the longer processing cycle. It didn't make me a lot of > friends > > in > > the grossing area though. > > > > For the biopsies you may want to be sure you have matching mesh > > cassettes. > > The lavender ones I usually always used mesh. > > > > Look at what your specimen types will be and associate them with > > something > > that is easily recognized by staff. There isn't a set protocol for it > > though. Sorry and I hope this helps. > > > > Vikki > > > > > > > > > > > > > > > > > > On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario < > > badzrosari@yahoo.com> wrote: > > > > > Good day histonetters.We are a new university hospital and setting > up > > > histopatholology lab.I used to work with white biopsy cassettes only > > but > > > not technicolors.Got this boss who ask me protocols on colored > > cassettes > > > etc...No idea about this.Is there any standard pattern which i can > > just > > > base and copy (example skin-yellow;breast-pink etc..)Im trying to > surf > > in > > > the net but cant find..Please someone help me??? > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marccentofante <@t> gmail.com Fri Mar 23 09:25:26 2012 From: marccentofante <@t> gmail.com (marc centofante) Date: Fri Mar 23 09:25:33 2012 Subject: [Histonet] Lab for sale Post from earlier Message-ID: About my previous post: My employer is looking to buy an anatomic pathology lab with frozen section service. Does anyone know of any labs for sale? I am located in FL and southeastern labs for sale is what we are interested in. Thanks in advance. -- Thank you, Marc Centofante marccentofante@gmail.com From sonya.martin <@t> soton.ac.uk Fri Mar 23 09:26:18 2012 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Fri Mar 23 09:26:39 2012 Subject: [Histonet] Murine Intestine Protocols (Lyn Stadler) In-Reply-To: <201203231412.q2NECq2P026662@mailgate4.iss.soton.ac.uk> References: <201203231412.q2NECq2P026662@mailgate4.iss.soton.ac.uk> Message-ID: <5BE0438A6D85E645A67A4A2F58F41693F67D@UOS-MSG00041-SI.soton.ac.uk> Are you doing paraffin or frozen? We do quite a lot of frozen colon / ileum so I can give you our protocols if you like. Sonya From gmartin <@t> marshallmedical.org Fri Mar 23 09:31:34 2012 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Fri Mar 23 09:31:43 2012 Subject: [Histonet] Cassette Protocol Message-ID: <6ED9D4252F278841A0593D3D788AF24C12EA0C9D@mailsvr.MARSHMED.local> It seems to me that this is somewhat guided by the funds you have to spend on a cassette labeler. Otherwise, if you label by hand, no problem. Also, the ordering factor can become a bit more detailed if you have a varied protocol. We keep it pretty simple; white being for single levels, greens for multiple levels, and yellow's for cytology. We do depend on our work list and the histologist skill to identify specimens and their possible special handling (IE; mamatome breast biopsy's = 5 levels). Gary Martin El Dorado Pathology From tpodawiltz <@t> lrgh.org Fri Mar 23 09:33:35 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Mar 23 09:33:45 2012 Subject: [Histonet] re: April fools prank In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93954D54D4@chimsx08.CHI.catholichealth.net> References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> <4940DF6D1C5FDF48931B6966AAEF93954D54D4@chimsx08.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB7E2732@LRGHEXVS1.practice.lrgh.org> The one that almost got us in a lot of trouble was the one that we had everyone convinced that I had been TDY'd to Quantico instead of going on leave. The best part about that one, was while the Officers in charge were running around trying to figure out who changed my orders from leave to TDY, no one thought to go to Sue's lab and ask her were I really was. The only thing that kept us from getting in trouble was none of the officers wanted to explain to the base Admiral why the fell for the joke. I still laugh when I think about that. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Thursday, March 22, 2012 2:40 PM To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank Tom, Not always a simple, fun prank, but bordering on the, I don't know......psycopathic? (I won't mention them, as someone might actually do them, then I'd have all those court appearences to deal with and phycological evaluations.... I just don't have that kind of time anymore) Of all the stunts we pulled, the ONLY one that ever got me in trouble was putting carbonated water in someones waterbath. Certainly one of the more benign efforts. I will likely do a lot of time in purgatory for some of those, mostly because they still cause me to smile, even after all these years. And to think we have become respected professionals and pillars of society. Who'd-a-thunk it? - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 22, 2012 1:20 PM To: Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From billodonnell <@t> catholichealth.net Fri Mar 23 09:39:51 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Mar 23 09:40:06 2012 Subject: [Histonet] re: April fools prank In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386324FB7E2732@LRGHEXVS1.practice.lrgh.org> References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> <4940DF6D1C5FDF48931B6966AAEF93954D54D4@chimsx08.CHI.catholichealth.net> <38667E7FB77ECD4E91BFAEB8D986386324FB7E2732@LRGHEXVS1.practice.lrgh.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93954D5548@chimsx08.CHI.catholichealth.net> We were safe as long as their egos were intact. -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Friday, March 23, 2012 9:34 AM To: O'Donnell, Bill; Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank The one that almost got us in a lot of trouble was the one that we had everyone convinced that I had been TDY'd to Quantico instead of going on leave. The best part about that one, was while the Officers in charge were running around trying to figure out who changed my orders from leave to TDY, no one thought to go to Sue's lab and ask her were I really was. The only thing that kept us from getting in trouble was none of the officers wanted to explain to the base Admiral why the fell for the joke. I still laugh when I think about that. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Thursday, March 22, 2012 2:40 PM To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank Tom, Not always a simple, fun prank, but bordering on the, I don't know......psycopathic? (I won't mention them, as someone might actually do them, then I'd have all those court appearences to deal with and phycological evaluations.... I just don't have that kind of time anymore) Of all the stunts we pulled, the ONLY one that ever got me in trouble was putting carbonated water in someones waterbath. Certainly one of the more benign efforts. I will likely do a lot of time in purgatory for some of those, mostly because they still cause me to smile, even after all these years. And to think we have become respected professionals and pillars of society. Who'd-a-thunk it? - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 22, 2012 1:20 PM To: Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From tpodawiltz <@t> lrgh.org Fri Mar 23 10:01:31 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Mar 23 10:01:41 2012 Subject: [Histonet] re: April fools prank In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93954D5548@chimsx08.CHI.catholichealth.net> References: , <2C40E43D1F7A56408C4463FD245DDDF997FBEAF8@EXCHMB-02.stowers-institute.org> <38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7@LRGHEXVS1.practice.lrgh.org> <4940DF6D1C5FDF48931B6966AAEF93954D54D4@chimsx08.CHI.catholichealth.net> <38667E7FB77ECD4E91BFAEB8D986386324FB7E2732@LRGHEXVS1.practice.lrgh.org> <4940DF6D1C5FDF48931B6966AAEF93954D5548@chimsx08.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB7E273C@LRGHEXVS1.practice.lrgh.org> So true, the person who found it the funniest was the Master Chief at Personnel, once he told me the whole story of what happened and wiped the laugh tears from his eyes. He warned me to keep a very, very, very low profile for awhile, which I passed on to you, Jesse and the rest of our crew. -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Friday, March 23, 2012 10:40 AM To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank We were safe as long as their egos were intact. -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Friday, March 23, 2012 9:34 AM To: O'Donnell, Bill; Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank The one that almost got us in a lot of trouble was the one that we had everyone convinced that I had been TDY'd to Quantico instead of going on leave. The best part about that one, was while the Officers in charge were running around trying to figure out who changed my orders from leave to TDY, no one thought to go to Sue's lab and ask her were I really was. The only thing that kept us from getting in trouble was none of the officers wanted to explain to the base Admiral why the fell for the joke. I still laugh when I think about that. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Thursday, March 22, 2012 2:40 PM To: Podawiltz, Thomas; Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank Tom, Not always a simple, fun prank, but bordering on the, I don't know......psycopathic? (I won't mention them, as someone might actually do them, then I'd have all those court appearences to deal with and phycological evaluations.... I just don't have that kind of time anymore) Of all the stunts we pulled, the ONLY one that ever got me in trouble was putting carbonated water in someones waterbath. Certainly one of the more benign efforts. I will likely do a lot of time in purgatory for some of those, mostly because they still cause me to smile, even after all these years. And to think we have become respected professionals and pillars of society. Who'd-a-thunk it? - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 22, 2012 1:20 PM To: Kara Lee; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: April fools prank I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Thursday, March 22, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: april fools prank One must always consider who the prank is being pulled on. If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled. As people working in the science industry, we have all been trained to observe. If a person is not the type to deal well with that type of humor, don't bother them and let them be. Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;) Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people. Cheers everyone! Kara > From: SLB@stowers.org > To: hmarlatt26@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 22 Mar 2012 07:14:41 -0500 > Subject: RE: [Histonet] re: april fools prank > CC: > > Heather, I see no harm in a fake spider. That would be fun. But, I totally agree with what Barry just wrote. Some of these other pranks are out of line, in my opinion. I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction. I have also pulled the hot dog trick on a resident pathologist. He loved it. > Sharon > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > heather marlatt > Sent: Wednesday, March 21, 2012 10:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] re: april fools prank > > I've been known to leave a fake spider in an embedder for the morning > person.....although it wasn't april fools just for fun it got a great > reaction :) _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From dreynold <@t> mdanderson.org Fri Mar 23 10:36:25 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Fri Mar 23 10:36:33 2012 Subject: [Histonet] RE: mouse abs on mouse tissue In-Reply-To: <2e127860-5449-4fd3-8e24-96725bae4200@DCPWPRTR03.mdanderson.edu> References: <2e127860-5449-4fd3-8e24-96725bae4200@DCPWPRTR03.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F0230C8872A1B@DCPWVMBXC0VS3.mdanderson.edu> I am not familiar with what IHC world has on this but we have used this technique for a number of years. The mouse fragment we use is from Jackson cat #115-007-003. We dilute it 1:10 in our protein blocking solution. It works better with DAB than with Fluorescence. We have also found that to get optimum blocking we need to incubate overnight at 4 degrees. This is more critical with some tissues and antibodies than with others. So we just routinely do it overnight if at all possible. If not we incubate for as long as possible. We usually get at least 98% blocking with this method. With fluorescence we us a 10% cold water fish gelatin (from Electron Microscopy) for our protein blocking solution. This really has cleaned up all our fluorescent labeling. With Dab our normal serum blocking is ok. You didn't say the dilution your were using for the Dylight. You may need to titer it out a little further. I have just recently learned that Jackson is also carrying the mouse Isotype specific antibodies with fluorescent dyes. We have always used the HRP isotype specific antibodies to help clean up our mouse abs on mouse tissue. I have ordered but not tried the fluorescent ones I am expecting good results. Donna Reynolds, ASCP Chief Histology Lab, U.T. M.D. Anderson Cancer Center, Houston TX Department Cancer Biology 713-792-8106 ------------------------------ Message: 20 Date: Fri, 23 Mar 2012 12:34:01 +0000 From: "James S." Subject: [Histonet] Mouse abs on mouse tissue method To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <5BE0438A6D85E645A67A4A2F58F41693F64A@UOS-MSG00041-SI.soton.ac.uk> Content-Type: text/plain; charset="us-ascii" I've been trying out the method from IHC world to use mouse primary plus anti-mouse secondary abs on mouse tissue. Protocol; Fresh frozen sections (mouse spleen) cut 8um and air dried Fixed acetone 10min Wash PBS Block with 2.5% normal goat serum in PBS/Tw, 30min Block with unconjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, diluted 1/10 in PBS/Tw), 2hrs room temp Wash PBS/Tw Seconary ab - DyLight488 conjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, 1/500 PBS/Tw), 20min room temp Wash PBS/Tw Mount Blocking with unconjugated Fab did decrease background if I used an IgG goat anti-mouse secondary but the background was actually increased when I used the Fab goat anti-mouse!! Any suggestions? Thanks Sonya From abilger <@t> wellspan.org Fri Mar 23 10:43:41 2012 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Fri Mar 23 10:44:21 2012 Subject: [Histonet] Intestine processing Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C9BD2DD99@EXCH02.wellspan.org> Lyn, If you are talking about small GI biopsies, we have a good protocol. We have a VIP tissue processor that has 3- formalins, 1- 70% ETOH, 1- 95% ETOH, 3- 100% ETOH's, 2-xylene's and 4- paraffins on it. Our GI biopsies are usually already fixed in formalin before we load them so we skip all the formalin steps. We do 5 minutes in all the other stations, 70% thru paraffins. We remove the cassettes ASAP from the processor so they do not sit in any heat for long. This dries them out. After embedding, blocks are faced off and allowed a brief time on ice with water on top. These specimens are not very dry so only need a short soak before cutting any levels. Sections are taken by cutting at a SLOW, even pace. We have been able to eliminate the "chatter" we used to see on GI biopsy slides by following this protocol. Hope this helps. Andrea Bilger CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From Jessica <@t> nsh.org Fri Mar 23 13:15:56 2012 From: Jessica <@t> nsh.org (Jessica Smith) Date: Fri Mar 23 13:16:06 2012 Subject: [Histonet] NSH 5th Annual Summer Symposium Message-ID: <2B6E973D7D12964F8D8A3598557C553004ED21@NSH-SRVR01.nsh.local> The National Society for Histotechnology is bringing the 5th Annual Summer Symposium back to Las Vegas, NV! General sessions and workshops featuring expert speakers will provide you with the tools, advice and guidance you seek in your professional career. The Summer Symposium is one of the best values for your limited training dollars in histology education offering 12 continuing education credits and an Exhibit Fair for one low price! Registration Fees: Member: $229 Non-Member: $249 Student Member: $169 HT Readiness Course Only (does not include exhibit fair): $99 This fantastic event will be held at the Planet Hollywood Resort & Casino right in the center of all the Las Vegas Entertainment! For more information regarding the agenda, travel information, and to register visit: http://s3.goeshow.com/nsh/SS2012/ereg419568.cfm?clear Or register by mail/fax: http://www.nsh.org/sites/default/files/Reg.%20Form.pdf Any questions or concerns please contact Jessica Smith at 443.535.4062 or jessica@nsh.org Jessica Smith Meeting Coordinator National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From one_angel_secret <@t> yahoo.com Fri Mar 23 14:00:20 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Mar 23 14:00:28 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: <1332529220.67695.YahooMailNeo@web112306.mail.gq1.yahoo.com> Bernadette, There isnt a set in stone formal by law guideline for this. Its a user preference. You can make up what ever colors you like for whatever criteria is specific to your lab. So as you see , you got different answers. Dont fret though, just figure out what your labs specific needs are and color code them to your liking. Good day. :) Kim D ________________________________ From: Bernadette del Rosario To: "histonet@lists.utsouthwestern.edu" Sent: Friday, March 23, 2012 8:42 AM Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES Good day histonetters.We are a ?new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern? which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail2 <@t> gmail.com Fri Mar 23 15:14:43 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Fri Mar 23 15:14:48 2012 Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES In-Reply-To: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> References: <1332506553.66638.YahooMailNeo@web130204.mail.mud.yahoo.com> Message-ID: It's pretty much up to you,. your boss, the pathologists what colors you use. Red for rushes for example. The colors help identify a group of specimens quickly, then they can be sorted by number. This is especially useful if your SOP is to cut groups of specimens in a specific order. Rena Fail On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario < badzrosari@yahoo.com> wrote: > Good day histonetters.We are a new university hospital and setting up > histopatholology lab.I used to work with white biopsy cassettes only but > not technicolors.Got this boss who ask me protocols on colored cassettes > etc...No idea about this.Is there any standard pattern which i can just > base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in > the net but cant find..Please someone help me??? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hlukey <@t> msn.com Fri Mar 23 18:57:42 2012 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Mar 23 18:57:47 2012 Subject: [Histonet] Tissue Micro Array Devices In-Reply-To: References: Message-ID: Hi Carol Freeman, I work in a TMA lab in Hawaii. We own, and highly recommend, Pathology Devices semi-automated TMArrayer and a Beecher TMA-1. However both are more expensive than the routine pathology lab can easily afford. If you Google "TMA arrayer" and you will get several links including, http://www.ihcworld.com/tissuearray.htm This information is nicely put together for various devices, and organizations with expertise, but seems a little dated. There are a few less expensive versions for TMA construction kits. Sakura's QuickRay was at NSH (http://www.sakura-americas.com/products/tisstek-quickray.html) and seems to be easier to keep the cores of tissue evenly lined up using a template that can be mounted and finally cut. I remember thinking it was pricey, but I don't remember the exact costs. Perhaps someone with a tissue arrayer can help you build some control blocks? For my hospital neighbors, I offered to make control blocks if they would loan me the blocks and slides with the areas circled that they want in their (recipient) blocks. I made a couple, but I find that labs need to make their own as quickly and easily as possible. Lets face it, for the quick/dirty method, you just need a razor blade and some some embedding molds. Therefore, for controls, I recommend you buy some "Biopsy Puches" (at least 3 mm) and make your own blocks using your embedding center (unless you understand the process of "Tempering"). Keep your control blocks simple to their needs, as people have a tendency of requesting exotic tumors in blocks that are depleted too quickly. Hugh UH Cancer Center Pathology shared resource Honolulu ------------------------------ Message: 4 Date: Fri, 23 Mar 2012 09:37:36 -0400 From: "Freeman, Carol" Subject: [Histonet] Tissue Micro Array Devices To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning and Happy Friday fellow histonetters :) I am looking into getting a Tissue Micro Array Device to make some nice multi-tissue (normal) and multi-tumor blocks for controls, validations and lot checks for IHC. Has anyone any advice for purchasing one, any favorites or any we should stay from....Any responses are appreciated. Thanks in advance ;) Carol Freeman From tuyenmai77 <@t> yahoo.com Sun Mar 25 02:50:31 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Sun Mar 25 02:50:36 2012 Subject: [Histonet] Re: Message-ID: <1332661831.68375.yint-ygo-j2me@web162803.mail.bf1.yahoo.com> Hi, friend! http://nopporngroup.com/contact.php?qiwep=15&aleki=753&fjxugef=94 From histotalk <@t> yahoo.com Mon Mar 26 06:41:50 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon Mar 26 06:41:56 2012 Subject: [Histonet] HistoTALK Welcomes VINNIE DELLA SPERANZa In-Reply-To: <1330351356.71660.YahooMailNeo@web120604.mail.ne1.yahoo.com> References: <1330351356.71660.YahooMailNeo@web120604.mail.ne1.yahoo.com> Message-ID: <1332762110.95981.YahooMailNeo@web120604.mail.ne1.yahoo.com> ? Hi NSH'ers - ? This Sunday's show, Program #24 of HistoTALK www.HistoTALK.com?welcomes?Vinnie Della Speranza, Manager for Anatomic Pathology Services?at?MUSC.?Vinnie needs no introduction! He has been a driving force in histotechnology and the NSH for many years and it was an honor having him on the show to talk about "professionalism" and being "professional". This is a terrific interview. Listen at home, at work (quietly) or anywhere you have an Internet connection! ? Yours, Dave From Sarah_Mack <@t> urmc.rochester.edu Mon Mar 26 08:28:58 2012 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Mon Mar 26 08:30:17 2012 Subject: [Histonet] Region I hotel deadline 3/28/12! Message-ID: The 2012 Region I meeting is approaching quickly! The venue will be April 27th and 28th at the Marriott Islandia Long Island. The hotel reservation deadline is March, 28, 2012! Please use the code NYSHSregionI to receive the group room rate. The convention room rate is $119.00 for single or double room. For reservations call: 1-800-228-9290 or go to http://www.marriott.com/hotels/travel/ispis-islandia-marriott-long-island/ Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-1702 From patjnm <@t> gwumc.edu Mon Mar 26 10:06:51 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Mon Mar 26 10:07:13 2012 Subject: [Histonet] Fite for Nocardio Message-ID: <4F704DCA.DB55.001F.1@gwumc.edu> I did this stain at AFIP with great success. Still use peanut oil and xylene, carbol fuchsin but instead of using acid alcohol with HCL, using sulfuric acid. Works great. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From Allison_Scott <@t> hchd.tmc.edu Mon Mar 26 10:55:11 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Mar 26 10:55:20 2012 Subject: [Histonet] Cross Contamination procedure flotation baths Message-ID: Hello to all in histoland. Does anyone have a cross contamination procedure for the water bath that they would be willing to share. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rjbuesa <@t> yahoo.com Mon Mar 26 11:07:54 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 26 11:08:01 2012 Subject: [Histonet] Cross Contamination procedure flotation baths In-Reply-To: Message-ID: <1332778074.51344.YahooMailClassic@web162104.mail.bf1.yahoo.com> Are you referring to how to prevent cross contamination? If that is the case, usually sectioning histotechs "wipe" clean the surface of the water in the flotation bath with a kin-wipe after each block has been cut and the slide taken. By doing so any section floating will be removed before the next block is sectioned. After sectioning is finished, the flotation bath is emptied and dry cleaned. Ren? J. --- On Mon, 3/26/12, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Cross Contamination procedure flotation baths To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 26, 2012, 11:55 AM Hello to all in histoland.? Does anyone have a cross contamination procedure for the water bath that they would be willing to share.? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Mon Mar 26 14:17:47 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Mar 26 14:18:04 2012 Subject: [Histonet] Re: Histonet Digest, Vol 100, Issue 37 In-Reply-To: <201203261702.q2QH2MvY019708@masdcsmrp112.kp.org> References: <201203261702.q2QH2MvY019708@masdcsmrp112.kp.org> Message-ID: How do you get on the web to listen to Vinnie? NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. histonet-request@lists.utsouthwestern.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 03/26/2012 10:02 AM Please respond to histonet@lists.utsouthwestern.edu To histonet@lists.utsouthwestern.edu cc Subject Histonet Digest, Vol 100, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. HistoTALK Welcomes VINNIE DELLA SPERANZa (David Kemler) 2. Region I hotel deadline 3/28/12! (Mack, Sarah) 3. Fite for Nocardio (Joseph Madary) 4. Cross Contamination procedure flotation baths (Scott, Allison D) 5. Re: Cross Contamination procedure flotation baths (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Mar 2012 04:41:50 -0700 (PDT) From: David Kemler Subject: [Histonet] HistoTALK Welcomes VINNIE DELLA SPERANZa To: Fellow HistoNetters Message-ID: <1332762110.95981.YahooMailNeo@web120604.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi NSH'ers - This Sunday's show, Program #24 of HistoTALK www.HistoTALK.com welcomes Vinnie Della Speranza, Manager for Anatomic Pathology Services at MUSC. Vinnie needs no introduction! He has been a driving force in histotechnology and the NSH for many years and it was an honor having him on the show to talk about "professionalism" and being "professional". This is a terrific interview. Listen at home, at work (quietly) or anywhere you have an Internet connection! Yours, Dave ------------------------------ Message: 2 Date: Mon, 26 Mar 2012 09:28:58 -0400 From: "Mack, Sarah" Subject: [Histonet] Region I hotel deadline 3/28/12! To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" The 2012 Region I meeting is approaching quickly! The venue will be April 27th and 28th at the Marriott Islandia Long Island. The hotel reservation deadline is March, 28, 2012! Please use the code NYSHSregionI to receive the group room rate. The convention room rate is $119.00 for single or double room. For reservations call: 1-800-228-9290 or go to http://www.marriott.com/hotels/travel/ispis-islandia-marriott-long-island/ Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-1702 ------------------------------ Message: 3 Date: Mon, 26 Mar 2012 11:06:51 -0400 From: "Joseph Madary" Subject: [Histonet] Fite for Nocardio To: Message-ID: <4F704DCA.DB55.001F.1@gwumc.edu> Content-Type: text/plain; charset="us-ascii" I did this stain at AFIP with great success. Still use peanut oil and xylene, carbol fuchsin but instead of using acid alcohol with HCL, using sulfuric acid. Works great. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ------------------------------ Message: 4 Date: Mon, 26 Mar 2012 15:55:11 +0000 From: "Scott, Allison D" Subject: [Histonet] Cross Contamination procedure flotation baths To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Does anyone have a cross contamination procedure for the water bath that they would be willing to share. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 5 Date: Mon, 26 Mar 2012 09:07:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cross Contamination procedure flotation baths To: "histonet@lists.utsouthwestern.edu" , Allison DScott Message-ID: <1332778074.51344.YahooMailClassic@web162104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Are you referring to how to prevent cross contamination? If that is the case, usually sectioning histotechs "wipe" clean the surface of the water in the flotation bath with a kin-wipe after each block has been cut and the slide taken. By doing so any section floating will be removed before the next block is sectioned. After sectioning is finished, the flotation bath is emptied and dry cleaned. Ren? J. --- On Mon, 3/26/12, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Cross Contamination procedure flotation baths To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 26, 2012, 11:55 AM Hello to all in histoland. Does anyone have a cross contamination procedure for the water bath that they would be willing to share. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 100, Issue 37 ***************************************** From Courtney.Pierce <@t> quintiles.com Mon Mar 26 14:56:02 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Mon Mar 26 14:56:09 2012 Subject: [Histonet] Stains for Cholesterol Esters Message-ID: Does anyone out in histo land know any stains for cholesterol or cholesterol esters? Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From rjbuesa <@t> yahoo.com Mon Mar 26 15:19:20 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 26 15:19:24 2012 Subject: [Histonet] Stains for Cholesterol Esters In-Reply-To: Message-ID: <1332793160.40904.YahooMailClassic@web162101.mail.bf1.yahoo.com> Courtney: Check Lillie's Histopathologic Technic and Practical Histochemistry" 3rd edition (1965) pages 473-474 and you will find what you are looking for. Ren? J. ? ? --- On Mon, 3/26/12, Courtney Pierce wrote: From: Courtney Pierce Subject: [Histonet] Stains for Cholesterol Esters To: "histonet@lists.utsouthwestern.edu" Date: Monday, March 26, 2012, 3:56 PM Does anyone out in histo land know any stains for cholesterol or cholesterol esters? Thanks Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital **********************? IMPORTANT--PLEASE READ? ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information.? If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited.? If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfray80 <@t> hotmail.com Mon Mar 26 17:48:16 2012 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Mon Mar 26 17:48:21 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com>, <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> Message-ID: Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." From pathlocums <@t> gmail.com Mon Mar 26 18:56:00 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Mar 26 18:56:04 2012 Subject: [Histonet] FW: Redneck Lent Message-ID: <5007488247871463800@unknownmsgid> Religious humor on this listserv is remarkably inappropriate. I cannot believe anyone would post this here. Tasteless. Sent from my Windows Phone From: JOSEPH FRAZEE Sent: 3/26/2012 3:49 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Mar 26 20:39:17 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Mar 26 20:39:43 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <5007488247871463800@unknownmsgid> References: <5007488247871463800@unknownmsgid> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A244D5@xmdb02.nch.kids> Yeah, But it is still funny (from a laugh-at-myself catholic) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, 27 March 2012 10:56 AM To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent Religious humor on this listserv is remarkably inappropriate. I cannot believe anyone would post this here. Tasteless. Sent from my Windows Phone From: JOSEPH FRAZEE Sent: 3/26/2012 3:49 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jfray80 <@t> hotmail.com Mon Mar 26 23:15:10 2012 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Mon Mar 26 23:15:13 2012 Subject: [Histonet] Histonet Server Message-ID: Sorry for the slip up it was not meant to go to the histonet. From louise.renton <@t> gmail.com Tue Mar 27 01:47:07 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Tue Mar 27 01:47:14 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> Message-ID: I have heard a local version here in South Africa - it just goes to show how information - good and bad is disseminated globally On Tue, Mar 27, 2012 at 12:48 AM, JOSEPH FRAZEE wrote: > > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; > yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; > jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root ; > Marjorie Norris ; "Tom Voss, Sr." < > tomvoss@wildblue.net>; Taber Stewart ; MONTIE L > WINTERS ; Terry Maloney ; > kerri spoering ; "Kenny & Debbie Hager" < > kanddhager@att.net> > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill and > cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the > priest sprinkled holy water over him, he said, "You were born a Baptist, > and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, and > the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed into > Bubba's yard, clutching a rosary and prepared to scold him, he stopped and > watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, > speak kindly." > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From meljdelbarrio <@t> yahoo.com Tue Mar 27 03:14:30 2012 From: meljdelbarrio <@t> yahoo.com (Mel John del Barrio) Date: Tue Mar 27 03:14:37 2012 Subject: [Histonet] Dehydrating Fluid articles Message-ID: <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> Hi All, I'm doing an essay about tissue processing and I'm having a ?very hard time looking for recent journal articles about the dehydrations step. I know that the dehydration step has been ?impervious to alterations ?but I'm just hoping that there are articles about it? Thanks MJ ? ? Image by FlamingText.com From lhadley <@t> iupui.edu Tue Mar 27 07:47:47 2012 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Tue Mar 27 07:47:59 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A244D5@xmdb02.nch.kids> References: <5007488247871463800@unknownmsgid> <6D6BD1DE8A5571489398B392A38A715760A244D5@xmdb02.nch.kids> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F2DD8751A@IU-MSSG-MBX110.ads.iu.edu> Ditto! People are so thinned skinned. Lee Ann Baldridge -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, March 26, 2012 9:39 PM To: 'Davide Costanzo'; JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent Yeah, But it is still funny (from a laugh-at-myself catholic) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, 27 March 2012 10:56 AM To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent Religious humor on this listserv is remarkably inappropriate. I cannot believe anyone would post this here. Tasteless. Sent from my Windows Phone From: JOSEPH FRAZEE Sent: 3/26/2012 3:49 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 27 08:07:16 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 27 08:07:23 2012 Subject: [Histonet] Dehydrating Fluid articles In-Reply-To: <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> Message-ID: <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> You are right about the fact that dehydration is a well known step. The most recent modifications deal with the use of different (and also?well known dehydrating agents) rather with the introduction of new ones. For your essay you will be better off by consulting some known histotechnique books. Bolles Lee, although very old, presents many dehydrating agents and their effects. Ren? J. --- On Tue, 3/27/12, Mel John del Barrio wrote: From: Mel John del Barrio Subject: [Histonet] Dehydrating Fluid articles To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 27, 2012, 4:14 AM Hi All, I'm doing an essay about tissue processing and I'm having a ?very hard time looking for recent journal articles about the dehydrations step. I know that the dehydration step has been ?impervious to alterations ?but I'm just hoping that there are articles about it? Thanks MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Tue Mar 27 08:30:59 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Mar 27 08:29:07 2012 Subject: [Histonet] CLIA requirements In-Reply-To: <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> CLIA requires me to keep a personnel file on everyone that works in the lab and I was wondering how you approach the staff about turning in their highest level of education. Do they bring in a copy of their HS diploma? College diploma? And what if they're not finished with college, do they only turn in their HS credit? Plus, copies of their certifications? From relia1 <@t> earthlink.net Tue Mar 27 09:05:07 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Mar 27 09:05:18 2012 Subject: [Histonet] Calling all Independent Lab Consultants setting up in house pathology labs. Message-ID: <14A7802EA7B44F0CAAAFA862D24B37D7@ownerf1abaad51> Are you a consultant that specializes in implementing in house pathology labs? If so please contact me at relia1@earthlink.net or 866-607-3542. I have some important information to share with you Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.twitter.com/pamatrelia From NMP <@t> stowers.org Tue Mar 27 09:09:13 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Tue Mar 27 09:09:25 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <5007488247871463800@unknownmsgid> References: <5007488247871463800@unknownmsgid> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF99BD3142B@EXCHMB-02.stowers-institute.org> For goodness sakes--lighten up. It was a funny 'joke' and I didn't see anything to get so upset about. I do see that if everyone did it then it could disrupt what 'Histonet' is supposed to be about so I guess any joke telling or anything not work related is inappropriate -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Monday, March 26, 2012 6:56 PM To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent Religious humor on this listserv is remarkably inappropriate. I cannot believe anyone would post this here. Tasteless. Sent from my Windows Phone From: JOSEPH FRAZEE Sent: 3/26/2012 3:49 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Tue Mar 27 09:09:50 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Mar 27 09:09:59 2012 Subject: [Histonet] CLIA requirements In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> References: <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> Message-ID: Hi Amber, Try contacting HR and the Compliance Officer (which may be your lab manager) as to how this requirement is handled. Institutions vary on this, but most times all employee files had to be with HR. Education, CEU's, certifications, licenses and transcripts, along with evaluations or disiplinary actions were all based there and nothing was to be taken out or copied. I've been at a few places recently where accessing of staff files was possible through the intranet - but only certain levels of supervision were able to actually access a part or all of them. Hope that this helps some. Vikki On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > > CLIA requires me to keep a personnel file on everyone that works in the > lab and I was wondering how you approach the staff about turning in their > highest level of education. Do they bring in a copy of their HS diploma? > College diploma? And what if they're not finished with college, do they > only turn in their HS credit? Plus, copies of their certifications? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tpodawiltz <@t> lrgh.org Tue Mar 27 09:46:07 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Mar 27 09:46:28 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF99BD3142B@EXCHMB-02.stowers-institute.org> References: <5007488247871463800@unknownmsgid> <2C40E43D1F7A56408C4463FD245DDDF99BD3142B@EXCHMB-02.stowers-institute.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB7E28AF@LRGHEXVS1.practice.lrgh.org> I help run an online first person shooter game server and one of the few rules we have is you need a thick skin to play here. The other few rules are No Racism, politics or religion discussions while playing. Come to have fun. To me the same should apply here. I come here for advice, sometimes give some, but mostly to learn. Work related jokes are fine, but I think we should avoid the hot buttons. Just stating my opinion. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Tuesday, March 27, 2012 10:09 AM To: 'Davide Costanzo'; JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent For goodness sakes--lighten up. It was a funny 'joke' and I didn't see anything to get so upset about. I do see that if everyone did it then it could disrupt what 'Histonet' is supposed to be about so I guess any joke telling or anything not work related is inappropriate -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Monday, March 26, 2012 6:56 PM To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent Religious humor on this listserv is remarkably inappropriate. I cannot believe anyone would post this here. Tasteless. Sent from my Windows Phone From: JOSEPH FRAZEE Sent: 3/26/2012 3:49 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From sbaldwin <@t> mhhcc.org Tue Mar 27 10:00:49 2012 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Mar 27 10:01:03 2012 Subject: [Histonet] CLIA requirements In-Reply-To: References: , <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> Message-ID: We ask out techs to bring in their transcripts, certifications ETC.. (they don't mind) and put a copy with their job descriptions so we have documentation when going thru inspections. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: Amber McKenzie From: Victoria Baker Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 03/27/2012 10:13AM Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] CLIA requirements Hi Amber, Try contacting HR and the Compliance Officer (which may be your lab manager) as to how this requirement is handled. Institutions vary on this, but most times all employee files had to be with HR. Education, CEU's, certifications, licenses and transcripts, along with evaluations or disiplinary actions were all based there and nothing was to be taken out or copied. I've been at a few places recently where accessing of staff files was possible through the intranet - but only certain levels of supervision were able to actually access a part or all of them. Hope that this helps some. Vikki On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > > CLIA requires me to keep a personnel file on everyone that works in the > lab and I was wondering how you approach the staff about turning in their > highest level of education. Do they bring in a copy of their HS diploma? > College diploma? And what if they're not finished with college, do they > only turn in their HS credit? Plus, copies of their certifications? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Tue Mar 27 10:08:52 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Mar 27 10:09:07 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com>, <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com>, Message-ID: <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From lhadley <@t> iupui.edu Tue Mar 27 10:15:00 2012 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Tue Mar 27 10:15:11 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com>, <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com>, <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F2DD8769B@IU-MSSG-MBX110.ads.iu.edu> Get 'em Debbie. My sentiments exactly! Lee Ann Baldridge -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 11:09 AM To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Mar 27 10:16:30 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Mar 27 10:16:45 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com>, <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com>, <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: AGREE! On Mar 27, 2012, at 8:08 AM, Boyd, Debbie M wrote: > For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] > Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pathlocums <@t> gmail.com Tue Mar 27 10:22:13 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Mar 27 10:22:21 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M wrote: > For goodness sakes! It is a joke. First of all it was accidently sent to > HistoNet per Joseph's second email. But most of all can't we just loosen > up a bit and laugh at/with each other? Every religion, race, gender, etc. > has had jokes made about it. Give the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical > Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [ > jfray80@hotmail.com] > Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; > yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; > jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root ; > Marjorie Norris ; "Tom Voss, Sr." < > tomvoss@wildblue.net>; Taber Stewart ; MONTIE L > WINTERS ; Terry Maloney ; > kerri spoering ; "Kenny & Debbie Hager" < > kanddhager@att.net> > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill and > cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the > priest sprinkled holy water over him, he said, "You were born a Baptist, > and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, and > the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed into > Bubba's yard, clutching a rosary and prepared to scold him, he stopped and > watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, > speak kindly." > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From Rcartun <@t> harthosp.org Tue Mar 27 10:22:39 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 27 10:22:51 2012 Subject: [Histonet] IHC for interferon gamma Message-ID: <4F71A2FF.7400.0077.1@harthosp.org> Has anyone succeeded in doing IHC for interferon gamma on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From DKBoyd <@t> chs.net Tue Mar 27 10:27:38 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Mar 27 10:27:52 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net>, Message-ID: <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> Your last sentence was inappropriate. Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________ From: Davide Costanzo [pathlocums@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote: For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray > To: Ruth Posey >; LueAnn Root >; Marjorie Norris >; "Tom Voss, Sr." >; Taber Stewart >; MONTIE L WINTERS >; Terry Maloney >; kerri spoering >; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- David Costanzo, MHS, PA (ASCP) Project Manager Blufrog Path Lab Solutions 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From SLB <@t> stowers.org Tue Mar 27 10:43:32 2012 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Tue Mar 27 10:43:40 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net>, <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> Hey how bout us rednecks? This redneck wasn't at all offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate. Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________ From: Davide Costanzo [pathlocums@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote: For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray > To: Ruth Posey >; LueAnn Root >; Marjorie Norris >; "Tom Voss, Sr." >; Taber Stewart >; MONTIE L WINTERS >; Terry Maloney >; kerri spoering >; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- David Costanzo, MHS, PA (ASCP) Project Manager Blufrog Path Lab Solutions 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Mar 27 10:55:54 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Mar 27 10:55:59 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com>, <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com>, <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: <1332863754.66364.YahooMailNeo@web5719.biz.mail.ne1.yahoo.com> Ditto!!! Joseph did apologize and said it was not meant to go to Histonet. I am sure everyone has hit the send button and quickly looked for the UNSEND button at one time or another. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Boyd, Debbie M" To: JOSEPH FRAZEE ; Histonet Server ; Taylors Cars ; LINDA FRAZEE ; mike & tony siltman Sent: Tuesday, March 27, 2012 10:08 AM Subject: RE: [Histonet] FW: Redneck Lent For goodness sakes!? It is a joke.? First of all it was accidently sent to HistoNet per Joseph's second email.? But most of all can't we just loosen up a bit and laugh at/with each other?? Every religion, race, gender, etc. has had jokes made about it.? Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist? l Southside Regional Medical Center l? 200 Medical Park Blvd.? l? Petersburg, Va.? 23805 l? PH 804-765-5050 l? FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jflinn <@t> gmu.edu Tue Mar 27 11:02:23 2012 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Mar 27 11:02:30 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <1332863754.66364.YahooMailNeo@web5719.biz.mail.ne1.yahoo.com> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> <1332863754.66364.YahooMailNeo@web5719.biz.mail.ne1.yahoo.com> Message-ID: I agree. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Paula Pierce Date: Tuesday, March 27, 2012 11:55 am Subject: Re: [Histonet] FW: Redneck Lent > Ditto!!! > > Joseph did apologize and said it was not meant to go to Histonet. > > > I am sure everyone has hit the send button and quickly looked for > the UNSEND button at one time or another. > > ? > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 8901 S. Santa Fe, Suite G > Oklahoma City, OK 73139 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: "Boyd, Debbie M" > To: JOSEPH FRAZEE ; Histonet Server > ; Taylors Cars > ; LINDA FRAZEE ; > mike & tony siltman > Sent: Tuesday, March 27, 2012 10:08 AM > Subject: RE: [Histonet] FW: Redneck Lent > > For goodness sakes!? It is a joke.? First of all it was accidently > sent to HistoNet per Joseph's second email.? But most of all can't > we just loosen up a bit and laugh at/with each other?? Every > religion, race, gender, etc. has had jokes made about it.? Give > the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist? l Southside Regional > Medical Center l? 200 Medical Park Blvd.? l? Petersburg, Va.? > 23805 l? PH 804-765-5050 l? FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet- > bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE > [jfray80@hotmail.com]Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; > yvette.fetterly@basf.com; footchina@yahoo.com; > frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root > ; Marjorie Norris ; "Tom > Voss, Sr." ; Taber Stewart > ; MONTIE L WINTERS ; > Terry Maloney ; kerri spoering > ; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor > grill and cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing > such a problem for the Catholic faithful that they finally talked > to their priest. > > The Priest came to visit Bubba, and suggested that he become a > Catholic. > After several classes and much study, Bubba attended Mass...and as > the priest sprinkled holy water over him, he said, "You were born > a Baptist, and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night > arrived, and the wonderful aroma of grilled venison filled the > neighborhood. > The Priest was called immediately by the neighbors, and, as he > rushed into Bubba's yard, clutching a rosary and prepared to scold > him, he stopped and watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care > deeply, speak kindly." > > > > > > ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? > _______________________________________________Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------- > ------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tuyenmai77 <@t> yahoo.com Tue Mar 27 11:23:20 2012 From: tuyenmai77 <@t> yahoo.com (Tuyen Nguyen) Date: Tue Mar 27 11:23:25 2012 Subject: [Histonet] Re[2]: Message-ID: <1332865400.39402.yint-ygo-j2me@web162802.mail.bf1.yahoo.com> Welcome, friend! http://loveboutiquesexshop.com/contact.php?yfupu=65&ugazylycy=167&myrolima=28 From hersheynut2000 <@t> yahoo.com Tue Mar 27 11:28:42 2012 From: hersheynut2000 <@t> yahoo.com (Mara Hernandez) Date: Tue Mar 27 11:28:52 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net>, <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> Message-ID: <1332865722.94705.YahooMailNeo@web181402.mail.ne1.yahoo.com> Awwwwwwwwwww, Joe......still the same 'ol Joe! I thought the joke was funny.? It's even funnier that you accidentally sent it all over Histonet!! ________________________________ From: "To: Davide Costanzo Cc:? Sent: Tuesday, March 27, 2012 10:43 AM Subject: RE: [Histonet] FW: Redneck Lent Hey how bout us rednecks?? This redneck wasn't at all offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate.? Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist? l Southside Regional Medical Center l? 200 Medical Park Blvd.? l? Petersburg, Va.? 23805 l? PH 804-765-5050 l? FAX 804-765-8852 ________________________________ From: Davide Costanzo [pathlocums@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote: For goodness sakes!? It is a joke.? First of all it was accidently sent to HistoNet per Joseph's second email.? But most of all can't we just loosen up a bit and laugh at/with each other?? Every religion, race, gender, etc. has had jokes made about it.? Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist? l Southside Regional Medical Center l? 200 Medical Park Blvd.? l? Petersburg, Va.? 23805 l? PH 804-765-5050 l? FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray > To: Ruth Posey >; LueAnn Root >; Marjorie Norris >; "Tom Voss, Sr." >; Taber Stewart >; MONTIE L WINTERS >; Terry Maloney >; kerri spoering >; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- David Costanzo, MHS, PA (ASCP) Project Manager Blufrog Path Lab Solutions 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hersheynut2000 <@t> yahoo.com Tue Mar 27 11:28:42 2012 From: hersheynut2000 <@t> yahoo.com (Mara Hernandez) Date: Tue Mar 27 11:29:51 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net>, <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> Message-ID: <1332865722.94705.YahooMailNeo@web181402.mail.ne1.yahoo.com> Awwwwwwwwwww, Joe......still the same 'ol Joe! I thought the joke was funny.? It's even funnier that you accidentally sent it all over Histonet!! ________________________________ From: "To: Davide Costanzo Cc:? Sent: Tuesday, March 27, 2012 10:43 AM Subject: RE: [Histonet] FW: Redneck Lent Hey how bout us rednecks?? This redneck wasn't at all offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate.? Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist? l Southside Regional Medical Center l? 200 Medical Park Blvd.? l? Petersburg, Va.? 23805 l? PH 804-765-5050 l? FAX 804-765-8852 ________________________________ From: Davide Costanzo [pathlocums@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote: For goodness sakes!? It is a joke.? First of all it was accidently sent to HistoNet per Joseph's second email.? But most of all can't we just loosen up a bit and laugh at/with each other?? Every religion, race, gender, etc. has had jokes made about it.? Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist? l Southside Regional Medical Center l? 200 Medical Park Blvd.? l? Petersburg, Va.? 23805 l? PH 804-765-5050 l? FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray > To: Ruth Posey >; LueAnn Root >; Marjorie Norris >; "Tom Voss, Sr." >; Taber Stewart >; MONTIE L WINTERS >; Terry Maloney >; kerri spoering >; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- David Costanzo, MHS, PA (ASCP) Project Manager Blufrog Path Lab Solutions 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Tue Mar 27 11:58:39 2012 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Tue Mar 27 11:58:44 2012 Subject: [Histonet] Re[2]: In-Reply-To: <1332865400.39402.yint-ygo-j2me@web162802.mail.bf1.yahoo.com> References: <1332865400.39402.yint-ygo-j2me@web162802.mail.bf1.yahoo.com> Message-ID: How about these postings to histonet? Is everyone else getting these from Tuyen Nguyen & Diamond Ranch Music? If so - can these people/email addresses be deleted from the histonet service? A little redneck humor is one thing - but the love boutique sex shop is another..... Jean Mitchell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen Sent: Tuesday, March 27, 2012 11:23 AM To: histonet@lists.utsouthwestern.edu; boosters@diamondranchmusic.org Subject: [Histonet] Re[2]: Welcome, friend! http://loveboutiquesexshop.com/contact.php?yfupu=65&ugazylycy=167&myroli ma=28 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karabou76 <@t> hotmail.com Tue Mar 27 12:05:52 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Tue Mar 27 12:06:06 2012 Subject: [Histonet] Re[2]: In-Reply-To: References: <1332865400.39402.yint-ygo-j2me@web162802.mail.bf1.yahoo.com>, Message-ID: I am getting these constantly as well. Very annoying. > Date: Tue, 27 Mar 2012 11:58:39 -0500 > From: JMitchell@uwhealth.org > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re[2]: > > How about these postings to histonet? Is everyone else getting these > from Tuyen Nguyen & Diamond Ranch Music? If so - can these people/email > addresses be deleted from the histonet service? > > A little redneck humor is one thing - but the love boutique sex shop is > another..... > > Jean Mitchell > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuyen > Nguyen > Sent: Tuesday, March 27, 2012 11:23 AM > To: histonet@lists.utsouthwestern.edu; boosters@diamondranchmusic.org > Subject: [Histonet] Re[2]: > > > Welcome, friend! > http://loveboutiquesexshop.com/contact.php?yfupu=65&ugazylycy=167&myroli > ma=28 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> umn.edu Tue Mar 27 12:12:23 2012 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Tue Mar 27 12:12:32 2012 Subject: [Histonet] Re[2]: In-Reply-To: References: <1332865400.39402.yint-ygo-j2me@web162802.mail.bf1.yahoo.com>, Message-ID: <4F71F4F7.8070804@umn.edu> I agree~~they need to be deleted from Histonet !! Getting them several times daily ! On 3/27/2012 12:05 PM, Kara Lee wrote: > I am getting these constantly as well. Very annoying. > >> Date: Tue, 27 Mar 2012 11:58:39 -0500 >> From: JMitchell@uwhealth.org >> To: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Re[2]: >> >> How about these postings to histonet? Is everyone else getting these >> from Tuyen Nguyen& Diamond Ranch Music? If so - can these people/email >> addresses be deleted from the histonet service? >> >> A little redneck humor is one thing - but the love boutique sex shop is >> another..... >> >> Jean Mitchell >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuyen >> Nguyen >> Sent: Tuesday, March 27, 2012 11:23 AM >> To: histonet@lists.utsouthwestern.edu; boosters@diamondranchmusic.org >> Subject: [Histonet] Re[2]: >> >> >> Welcome, friend! >> http://loveboutiquesexshop.com/contact.php?yfupu=65&ugazylycy=167&myroli >> ma=28 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Tue Mar 27 12:25:57 2012 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Tue Mar 27 12:26:16 2012 Subject: [Histonet] RE: Redneck Lent In-Reply-To: References: Message-ID: Okay - ya'll seriously need to not read too much into the Catholic thing about that joke - I'm from Kentucky and I think it's more about the 'redneck' thing than a religion!!! I thought it was hilarious - course, I'm not Catholic - but I can always laugh at a redneck joke!! Happy Tuesday ya'll!!! Oh yeah - and the puppies are getting' big in Indiana - if ya' know what this means then I'm saying HI to ya' !!! Kristie This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From one_angel_secret <@t> yahoo.com Tue Mar 27 13:07:53 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 27 13:08:00 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> Message-ID: <8F3AC350-2097-44A8-9FD8-46F289956DA4@yahoo.com> Ahhh lol. I love it :) Sent from my iPhone On Mar 26, 2012, at 6:48 PM, JOSEPH FRAZEE wrote: > > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Tue Mar 27 12:50:54 2012 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Tue Mar 27 13:24:07 2012 Subject: [Histonet] Re[2]: In-Reply-To: <4F71F4F7.8070804@umn.edu> References: <1332865400.39402.yint-ygo-j2me@web162802.mail.bf1.yahoo.com>, , , , <4F71F4F7.8070804@umn.edu> Message-ID: Their email accounts have been hacked and all their addresses stolen. They need to report and change email login and password. This happened to me last year and I was sending out Viagra deals to all my contacts including histonet. > Date: Tue, 27 Mar 2012 12:12:23 -0500 > From: ander093@umn.edu > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re[2]: > > I agree~~they need to be deleted from Histonet !! Getting them several > times daily ! > > > On 3/27/2012 12:05 PM, Kara Lee wrote: > > I am getting these constantly as well. Very annoying. > > > >> Date: Tue, 27 Mar 2012 11:58:39 -0500 > >> From: JMitchell@uwhealth.org > >> To: histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] Re[2]: > >> > >> How about these postings to histonet? Is everyone else getting these > >> from Tuyen Nguyen& Diamond Ranch Music? If so - can these people/email > >> addresses be deleted from the histonet service? > >> > >> A little redneck humor is one thing - but the love boutique sex shop is > >> another..... > >> > >> Jean Mitchell > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tuyen > >> Nguyen > >> Sent: Tuesday, March 27, 2012 11:23 AM > >> To: histonet@lists.utsouthwestern.edu; boosters@diamondranchmusic.org > >> Subject: [Histonet] Re[2]: > >> > >> > >> Welcome, friend! > >> http://loveboutiquesexshop.com/contact.php?yfupu=65&ugazylycy=167&myroli > >> ma=28 > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leswes <@t> shaw.ca Tue Mar 27 13:26:26 2012 From: leswes <@t> shaw.ca (Lesley Weston) Date: Tue Mar 27 13:26:31 2012 Subject: [Histonet] FW: Redneck Lent Message-ID: <7C5BDE41-AD70-43F3-B9DB-C59ECC023D74@shaw.ca> It is indeed a joke, an oldie but a goodie. I first heard it years ago about the Medieval Portuguese Jews who converted to Christianity as the only alternative to death at the hands of the Inquisition. The Bishop made a surprise visit to a "convert's" house during Friday-night-supper, so the householder sprinkled water on the roast chicken, saying "You're fish, you're fish, you're fish." Lesley. > For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] > Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com;donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patjnm <@t> gwumc.edu Tue Mar 27 13:31:20 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Tue Mar 27 13:31:27 2012 Subject: [Histonet] i thought the joke was funny Message-ID: <4F71CF37.DB55.001F.1@gwumc.edu> Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From leswes <@t> shaw.ca Tue Mar 27 13:44:06 2012 From: leswes <@t> shaw.ca (Lesley Weston) Date: Tue Mar 27 13:44:12 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: You're right about your second paragraph. I tried to reply with the original joke, which is indeed about people of the Mosaic faith (only I used the more usual term) surviving the Inquisition, but I got this reply: > This email has violated the RACIAL DISCRIMINATION. > and Quarantine entire message has been taken on 3/27/2012 2:29:28 PM. > Message details: > Server: BCHEXEG > Sender: leswes@shaw.ca; > Recipient: Histonet@lists.utsouthwestern.edu; > Subject: RE: [Histonet] FW: Redneck Lent As a J-person, I ought to be be deeply offended, but the whole thing is just getting funnier and funnier. Lesley. On 03-27-2012, at 8:22 AM, Davide Costanzo wrote: > I, personally, found the joke very funny. I find a lot of distasteful jokes > funny - I actually prefer them over anything clean. That does not take away > the fact that discussing religion, or politics (with humor or in any other > form) has no place in the workplace. Histonet is, in many ways, an > extension of the workplace. I also do not discuss religion or politics with > strangers, and there certainly are more strangers that read this blog than > folks we know. While I was not personally offended by that joke, it is very > conceivable to think that some folks would be offended. > > As I told one replier - had this joke been about Jews it would have been > something folks reacted to harshly. And, for good reason. So we cannot joke > about Jews or Muslims, but Catholics are fine? I respectfully disagree - > ALL religions and posts of humor in reference to a religion on a public > listserv is a terrible idea. > > And, incidentally, this support for those that could be offended is coming > from me - a person that thinks ALL religion is a joke in the first place. > > > > On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M wrote: > >> For goodness sakes! It is a joke. First of all it was accidently sent to >> HistoNet per Joseph's second email. But most of all can't we just loosen >> up a bit and laugh at/with each other? Every religion, race, gender, etc. >> has had jokes made about it. Give the guy a break. >> >> Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical >> Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH >> 804-765-5050 l FAX 804-765-8852 >> >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu [ >> histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [ >> jfray80@hotmail.com] >> Sent: Monday, March 26, 2012 6:48 PM >> To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman >> Subject: [Histonet] FW: Redneck Lent >> >> Date: Mon, 26 Mar 2012 19:55:27 +0100 >> From: spoeringk@yahoo.com >> Subject: Fw: Fwd: Redneck Lent >> To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; >> yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; >> jfray80@hotmail.com; donna.lueck@gmail.com >> >> >> >> >> Kerri >> >> >> >> ----- Forwarded Message ----- >> From: Sharen Pray >> To: Ruth Posey ; LueAnn Root ; >> Marjorie Norris ; "Tom Voss, Sr." < >> tomvoss@wildblue.net>; Taber Stewart ; MONTIE L >> WINTERS ; Terry Maloney ; >> kerri spoering ; "Kenny & Debbie Hager" < >> kanddhager@att.net> >> Sent: Saturday, 24 March 2012, 21:06 >> Subject: Fw: Fwd: Redneck Lent >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Each Friday night after work, Bubba would fire up his outdoor grill and >> cook a venison steak. But, all of >> >> >> >> >> >> Bubba's neighbors were Catholic. And since it was Lent, they were >> forbidden from eating meat on Friday. >> >> >> The delicious aroma from the grilled venison steaks was causing such a >> problem for the Catholic faithful that they finally talked to their priest. >> >> The Priest came to visit Bubba, and suggested that he become a Catholic. >> >> After several classes and much study, Bubba attended Mass...and as the >> priest sprinkled holy water over him, he said, "You were born a Baptist, >> and raised a Baptist, but now you are a Catholic." >> >> Bubba's neighbors were greatly relieved, until Friday night arrived, and >> the wonderful aroma of grilled venison filled the neighborhood. >> >> The Priest was called immediately by the neighbors, and, as he rushed into >> Bubba's yard, clutching a rosary and prepared to scold him, he stopped and >> watched in amazement. >> >> There stood Bubba, clutching a small bottle of holy water which he >> carefully sprinkled over the grilling meat and chanted: >> "You wuz born a >> deer, you wuz raised a deer, but now you is a catfish." >> >> >> >> Blessings, love and light, "Live simply, love generously, care deeply, >> speak kindly." >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> -------------------------------------------------------------------------- >> Disclaimer: This electronic message may contain information that is >> Proprietary, Confidential, or legally privileged or protected. It >> is intended only for the use of the individual(s) and entity named >> in the message. If you are not an intended recipient of this >> message, please notify the sender immediately and delete the >> material from your computer. Do not deliver, distribute or copy >> this message and do not disclose its contents or take any action in >> reliance on the information it contains. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCBRITTON <@t> Cheshire-Med.COM Tue Mar 27 14:16:46 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Tue Mar 27 14:16:42 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com><1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com><1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com><7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net>, <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> Message-ID: I am a redneck and a catholic and was not offended at all! As a matter of fact it made me hungry! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Tuesday, March 27, 2012 11:44 AM To: Davide Costanzo Cc: Histonet Server Subject: RE: [Histonet] FW: Redneck Lent Hey how bout us rednecks? This redneck wasn't at all offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate. Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________ From: Davide Costanzo [pathlocums@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote: For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray > To: Ruth Posey >; LueAnn Root >; Marjorie Norris >; "Tom Voss, Sr." >; Taber Stewart >; MONTIE L WINTERS >; Terry Maloney >; kerri spoering >; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- David Costanzo, MHS, PA (ASCP) Project Manager Blufrog Path Lab Solutions 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Mar 27 14:36:23 2012 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Mar 27 14:36:34 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com><1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com><1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com><7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net>, <7EAFE982E328304DA6CE2B677BB7624601C980A2@TN001WEXMBX12.US.chs.net> <2C40E43D1F7A56408C4463FD245DDDF99BC60C04@EXCHMB-02.stowers-institute.org> Message-ID: <4F71DE77.2B7F.00C9.1@geisinger.edu> I'm a redneck Catholic too and I heard it weeks ago and thought it was pretty funny. Still doesn't belong on a public forum though. >>> "Britton, Josette C" 3/27/2012 3:16 PM >>> I am a redneck and a catholic and was not offended at all! As a matter of fact it made me hungry! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Tuesday, March 27, 2012 11:44 AM To: Davide Costanzo Cc: Histonet Server Subject: RE: [Histonet] FW: Redneck Lent Hey how bout us rednecks? This redneck wasn't at all offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate. Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________ From: Davide Costanzo [pathlocums@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote: For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray > To: Ruth Posey >; LueAnn Root >; Marjorie Norris >; "Tom Voss, Sr." >; Taber Stewart >; MONTIE L WINTERS >; Terry Maloney >; kerri spoering >; "Kenny & Debbie Hager" > Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- David Costanzo, MHS, PA (ASCP) Project Manager Blufrog Path Lab Solutions 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From jflinn <@t> gmu.edu Tue Mar 27 14:47:47 2012 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Mar 27 14:47:50 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: Me too. Are we all in violation? jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Lesley Weston Date: Tuesday, March 27, 2012 2:44 pm Subject: Re: [Histonet] FW: Redneck Lent > You're right about your second paragraph. I tried to reply with > the original joke, which is indeed about people of the Mosaic > faith (only I used the more usual term) surviving the Inquisition, > but I got this reply: > > > > This email has violated the RACIAL DISCRIMINATION. > > and Quarantine entire message has been taken on 3/27/2012 > 2:29:28 PM. > > Message details: > > Server: BCHEXEG > > Sender: leswes@shaw.ca; > > Recipient: Histonet@lists.utsouthwestern.edu; > > Subject: RE: [Histonet] FW: Redneck Lent > > As a J-person, I ought to be be deeply offended, but the whole > thing is just getting funnier and funnier. > > Lesley. > > > On 03-27-2012, at 8:22 AM, Davide Costanzo wrote: > > > I, personally, found the joke very funny. I find a lot of > distasteful jokes > > funny - I actually prefer them over anything clean. That does > not take away > > the fact that discussing religion, or politics (with humor or in > any other > > form) has no place in the workplace. Histonet is, in many ways, an > > extension of the workplace. I also do not discuss religion or > politics with > > strangers, and there certainly are more strangers that read this > blog than > > folks we know. While I was not personally offended by that joke, > it is very > > conceivable to think that some folks would be offended. > > > > As I told one replier - had this joke been about Jews it would > have been > > something folks reacted to harshly. And, for good reason. So we > cannot joke > > about Jews or Muslims, but Catholics are fine? I respectfully > disagree - > > ALL religions and posts of humor in reference to a religion on a > public> listserv is a terrible idea. > > > > And, incidentally, this support for those that could be offended > is coming > > from me - a person that thinks ALL religion is a joke in the > first place. > > > > > > > > On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M > wrote:> > >> For goodness sakes! It is a joke. First of all it was > accidently sent to > >> HistoNet per Joseph's second email. But most of all can't we > just loosen > >> up a bit and laugh at/with each other? Every religion, race, > gender, etc. > >> has had jokes made about it. Give the guy a break. > >> > >> Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside > Regional Medical > >> Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH > >> 804-765-5050 l FAX 804-765-8852 > >> > >> ________________________________________ > >> From: histonet-bounces@lists.utsouthwestern.edu [ > >> histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH > FRAZEE [ > >> jfray80@hotmail.com] > >> Sent: Monday, March 26, 2012 6:48 PM > >> To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony > siltman>> Subject: [Histonet] FW: Redneck Lent > >> > >> Date: Mon, 26 Mar 2012 19:55:27 +0100 > >> From: spoeringk@yahoo.com > >> Subject: Fw: Fwd: Redneck Lent > >> To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; > >> yvette.fetterly@basf.com; footchina@yahoo.com; > frazeelinda@hotmail.com;>> jfray80@hotmail.com; donna.lueck@gmail.com > >> > >> > >> > >> > >> Kerri > >> > >> > >> > >> ----- Forwarded Message ----- > >> From: Sharen Pray > >> To: Ruth Posey ; LueAnn Root > ;>> Marjorie Norris ; > "Tom Voss, Sr." < > >> tomvoss@wildblue.net>; Taber Stewart ; > MONTIE L > >> WINTERS ; Terry Maloney > ;>> kerri spoering ; > "Kenny & Debbie Hager" < > >> kanddhager@att.net> > >> Sent: Saturday, 24 March 2012, 21:06 > >> Subject: Fw: Fwd: Redneck Lent > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> Each Friday night after work, Bubba would fire up his outdoor > grill and > >> cook a venison steak. But, all of > >> > >> > >> > >> > >> > >> Bubba's neighbors were Catholic. And since it was Lent, they were > >> forbidden from eating meat on Friday. > >> > >> > >> The delicious aroma from the grilled venison steaks was causing > such a > >> problem for the Catholic faithful that they finally talked to > their priest. > >> > >> The Priest came to visit Bubba, and suggested that he become a > Catholic.>> > >> After several classes and much study, Bubba attended Mass...and > as the > >> priest sprinkled holy water over him, he said, "You were born a > Baptist,>> and raised a Baptist, but now you are a Catholic." > >> > >> Bubba's neighbors were greatly relieved, until Friday night > arrived, and > >> the wonderful aroma of grilled venison filled the neighborhood. > >> > >> The Priest was called immediately by the neighbors, and, as he > rushed into > >> Bubba's yard, clutching a rosary and prepared to scold him, he > stopped and > >> watched in amazement. > >> > >> There stood Bubba, clutching a small bottle of holy water which he > >> carefully sprinkled over the grilling meat and chanted: > >> "You wuz born a > >> deer, you wuz raised a deer, but now you is a catfish." > >> > >> > >> > >> Blessings, love and light, "Live simply, love generously, care > deeply,>> speak kindly." > >> > >> > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> ---------------------------------------------------------------- > ---------- > >> Disclaimer: This electronic message may contain information > that is > >> Proprietary, Confidential, or legally privileged or protected. It > >> is intended only for the use of the individual(s) and entity named > >> in the message. If you are not an intended recipient of this > >> message, please notify the sender immediately and delete the > >> material from your computer. Do not deliver, distribute or copy > >> this message and do not disclose its contents or take any > action in > >> reliance on the information it contains. > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > > -- > > *David Costanzo, MHS, PA (ASCP)* > > Project Manager > > *Blufrog Path Lab Solutions* > > 9401 Wilshire Blvd. Ste 650 > > Beverly Hills, CA 90212 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amber.mckenzie <@t> gastrodocs.net Tue Mar 27 15:11:10 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Mar 27 15:09:16 2012 Subject: [Histonet] CLIA requirements In-Reply-To: References: , <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC116309B8@JERRY.Gia.com> About the personnel files, do you keep these in the lab on each employee plus what HR has or do you just call HR when CLIA arrives and let HR handle all the personnel questions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, March 27, 2012 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA requirements We ask out techs to bring in their transcripts, certifications ETC.. (they don't mind) and put a copy with their job descriptions so we have documentation when going thru inspections. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: Amber McKenzie From: Victoria Baker Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 03/27/2012 10:13AM Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] CLIA requirements Hi Amber, Try contacting HR and the Compliance Officer (which may be your lab manager) as to how this requirement is handled. Institutions vary on this, but most times all employee files had to be with HR. Education, CEU's, certifications, licenses and transcripts, along with evaluations or disiplinary actions were all based there and nothing was to be taken out or copied. I've been at a few places recently where accessing of staff files was possible through the intranet - but only certain levels of supervision were able to actually access a part or all of them. Hope that this helps some. Vikki On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > > CLIA requires me to keep a personnel file on everyone that works in the > lab and I was wondering how you approach the staff about turning in their > highest level of education. Do they bring in a copy of their HS diploma? > College diploma? And what if they're not finished with college, do they > only turn in their HS credit? Plus, copies of their certifications? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Mar 27 15:27:22 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 27 15:27:07 2012 Subject: [Histonet] CLIA requirements In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC116309B8@JERRY.Gia.com> References: , <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> <5A33C952BB67F4468AF1F36D739212BC116309B8@JERRY.Gia.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FB9922@SMCMAIL01.somerset-healthcare.com> We keep all of ours in the lab. Much easier than having HR search their files for the documentation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, March 27, 2012 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA requirements About the personnel files, do you keep these in the lab on each employee plus what HR has or do you just call HR when CLIA arrives and let HR handle all the personnel questions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, March 27, 2012 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA requirements We ask out techs to bring in their transcripts, certifications ETC.. (they don't mind) and put a copy with their job descriptions so we have documentation when going thru inspections. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: Amber McKenzie From: Victoria Baker Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 03/27/2012 10:13AM Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] CLIA requirements Hi Amber, Try contacting HR and the Compliance Officer (which may be your lab manager) as to how this requirement is handled. Institutions vary on this, but most times all employee files had to be with HR. Education, CEU's, certifications, licenses and transcripts, along with evaluations or disiplinary actions were all based there and nothing was to be taken out or copied. I've been at a few places recently where accessing of staff files was possible through the intranet - but only certain levels of supervision were able to actually access a part or all of them. Hope that this helps some. Vikki On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > > CLIA requires me to keep a personnel file on everyone that works in the > lab and I was wondering how you approach the staff about turning in their > highest level of education. Do they bring in a copy of their HS diploma? > College diploma? And what if they're not finished with college, do they > only turn in their HS credit? Plus, copies of their certifications? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Eric.Hoy <@t> UTSouthwestern.edu Tue Mar 27 15:45:39 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Tue Mar 27 15:45:49 2012 Subject: [Histonet] An immunologist... Message-ID: An immunologist, a histologist, and a microbiologist go into a bar.... I guess I?d better not finish this, because the political-correctness police will come after me. Anyway, it made me laugh, and I?ve worked in all three of those professions. Life is wonderful... why do so many people here need to get one? Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== From jessica <@t> prometheushealthcare.com Tue Mar 27 15:45:45 2012 From: jessica <@t> prometheushealthcare.com (Jessica-Prometheus) Date: Tue Mar 27 15:45:53 2012 Subject: [Histonet] Histology Supervisor Opening! Message-ID: <00ed01cd0c5a$9524f6b0$bf6ee410$@prometheushealthcare.com> Hey, I specialize in the recruitment of lab professionals in Maryland, and I'm looking to fill a full time, day time Histology Supervisor position at a leading hospital in Leonardtown, MD. The ideal candidate would have a Bachelors, Masters or Doctoral in Clinical Lab Sciences or Medical Technology, must be ASCP/AMT/HHS/NCA certified and 5 years of experience in the area of Histology with supervisor experience. The company offers a very competitive salary, full benefits, and would offer relocation. If you are interested or know anyone who might be, please contact me. Please feel free to pass along this information as well. For immediate consideration, just reply back with your most up to date resume. Thank you! Jessica Sanchez Account Manager Prometheus Healthcare Office 301-693-9057 Direct 301-693-8908 Fax 301-368-2478 jessica@prometheushealthcare.com www.prometheushealthcare.com From amber.mckenzie <@t> gastrodocs.net Tue Mar 27 16:00:33 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Mar 27 15:58:40 2012 Subject: [Histonet] CLIA requirements In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FB9922@SMCMAIL01.somerset-healthcare.com> References: , <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> <5A33C952BB67F4468AF1F36D739212BC116309B8@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711FB9922@SMCMAIL01.somerset-healthcare.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC11630A02@JERRY.Gia.com> Do you make it a requirement to work in the lab that everyone provide proof of education b/c it's not a company policy where I work. Plus, does CLIA state in their website about what they require in the personnel files? Is it just employee start date, highest level of education, evals and proficiency testing? -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Tuesday, March 27, 2012 3:27 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA requirements We keep all of ours in the lab. Much easier than having HR search their files for the documentation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, March 27, 2012 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA requirements About the personnel files, do you keep these in the lab on each employee plus what HR has or do you just call HR when CLIA arrives and let HR handle all the personnel questions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, March 27, 2012 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA requirements We ask out techs to bring in their transcripts, certifications ETC.. (they don't mind) and put a copy with their job descriptions so we have documentation when going thru inspections. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: Amber McKenzie From: Victoria Baker Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 03/27/2012 10:13AM Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] CLIA requirements Hi Amber, Try contacting HR and the Compliance Officer (which may be your lab manager) as to how this requirement is handled. Institutions vary on this, but most times all employee files had to be with HR. Education, CEU's, certifications, licenses and transcripts, along with evaluations or disiplinary actions were all based there and nothing was to be taken out or copied. I've been at a few places recently where accessing of staff files was possible through the intranet - but only certain levels of supervision were able to actually access a part or all of them. Hope that this helps some. Vikki On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > > CLIA requires me to keep a personnel file on everyone that works in the > lab and I was wondering how you approach the staff about turning in their > highest level of education. Do they bring in a copy of their HS diploma? > College diploma? And what if they're not finished with college, do they > only turn in their HS credit? Plus, copies of their certifications? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From rsrichmond <@t> gmail.com Tue Mar 27 16:13:32 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Mar 27 16:13:36 2012 Subject: [Histonet] Re: Redneck Lent Message-ID: I think this thread is the longest I've seen in fifteen years on Histonet! I find the joke a little lame, but the thread gets funnier and funnier. If the joke gives offense, it's because its writer clearly has only a very dim idea of Catholic practice (either Roman or Anglican - I'm an Episcopalian). It would be lot funnier if these details were rewritten. Lesley Weston then tried to tell a comparable story from Jewish history, only to have it intercepted by his corporate netnanny, apparently because it contained the word J-w. I think of playwright Herman Wouk's book about his Orthodox faith he published about 50 years ago - "This Is My God" is I think the most profound work of religious apologetic written in the 20th century - well, maybe C.S. Lewis's "Mere Christianity" - Wouk the playwright wryly observed that the word J-w uttered on the stage is always a shocker. As an aside, when you forward anything on the Web, make sure always to delete all the accumulated e-mail addresses in it - they're an invitation to spammers. Bob Richmond Samurai Pathologist Knoxville TN From leswes <@t> shaw.ca Tue Mar 27 16:30:35 2012 From: leswes <@t> shaw.ca (Lesley Weston) Date: Tue Mar 27 16:30:41 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <2857010BC481413CA3FD6CE0FF89C811@ownerf1abaad51> References: <7C5BDE41-AD70-43F3-B9DB-C59ECC023D74@shaw.ca> <2857010BC481413CA3FD6CE0FF89C811@ownerf1abaad51> Message-ID: <6CFE7A01-76A0-4C81-8B67-3EEED978B672@shaw.ca> Me too! And you're welcome. Lesley. On 03-27-2012, at 11:37 AM, Pam Barker wrote: > Hahahahahaha!!!! (Im Jewish!) > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley > Weston > Sent: Tuesday, March 27, 2012 2:26 PM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] FW: Redneck Lent > > It is indeed a joke, an oldie but a goodie. I first heard it years ago about > the Medieval Portuguese Jews who converted to Christianity as the only > alternative to death at the hands of the Inquisition. The Bishop made a > surprise visit to a "convert's" house during Friday-night-supper, so the > householder sprinkled water on the roast chicken, saying "You're fish, > you're fish, you're fish." > > Lesley. > >> For goodness sakes! It is a joke. First of all it was accidently sent to > HistoNet per Joseph's second email. But most of all can't we just loosen up > a bit and laugh at/with each other? Every religion, race, gender, etc. has > had jokes made about it. Give the guy a break. >> >> Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional >> Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l >> PH 804-765-5050 l FAX 804-765-8852 >> >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE >> [jfray80@hotmail.com] >> Sent: Monday, March 26, 2012 6:48 PM >> To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman >> Subject: [Histonet] FW: Redneck Lent >> >> Date: Mon, 26 Mar 2012 19:55:27 +0100 >> From: spoeringk@yahoo.com >> Subject: Fw: Fwd: Redneck Lent >> To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; >> yvette.fetterly@basf.com; footchina@yahoo.com; >> frazeelinda@hotmail.com; jfray80@hotmail.com;donna.lueck@gmail.com >> >> >> >> >> Kerri >> >> >> >> ----- Forwarded Message ----- >> From: Sharen Pray >> To: Ruth Posey ; LueAnn Root >> ; Marjorie Norris ; "Tom >> Voss, Sr." ; Taber Stewart >> ; MONTIE L WINTERS ; Terry >> Maloney ; kerri spoering >> ; "Kenny & Debbie Hager" >> Sent: Saturday, 24 March 2012, 21:06 >> Subject: Fw: Fwd: Redneck Lent >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Each Friday night after work, Bubba would fire up his outdoor grill >> and cook a venison steak. But, all of >> >> >> >> >> >> Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. >> >> >> The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. >> >> The Priest came to visit Bubba, and suggested that he become a Catholic. >> >> After several classes and much study, Bubba attended Mass...and as the > priest sprinkled holy water over him, he said, "You were born a Baptist, and > raised a Baptist, but now you are a Catholic." >> >> Bubba's neighbors were greatly relieved, until Friday night arrived, and > the wonderful aroma of grilled venison filled the neighborhood. >> >> The Priest was called immediately by the neighbors, and, as he rushed into > Bubba's yard, clutching a rosary and prepared to scold him, he stopped and > watched in amazement. >> >> There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: >> "You wuz born a >> deer, you wuz raised a deer, but now you is a catfish." >> >> >> >> Blessings, love and light, "Live simply, love generously, care deeply, > speak kindly." >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ---------------------------------------------------------------------- >> ---- >> Disclaimer: This electronic message may contain information that is >> Proprietary, Confidential, or legally privileged or protected. It is >> intended only for the use of the individual(s) and entity named in the >> message. If you are not an intended recipient of this message, please >> notify the sender immediately and delete the material from your >> computer. Do not deliver, distribute or copy this message and do not >> disclose its contents or take any action in reliance on the >> information it contains. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From one_angel_secret <@t> yahoo.com Tue Mar 27 16:34:04 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 27 16:34:12 2012 Subject: [Histonet] Re: Redneck Lent In-Reply-To: References: Message-ID: Good to see some of us still have a sense of humor. We are the sultans of swing :D Sent from my iPhone On Mar 27, 2012, at 5:13 PM, Bob Richmond wrote: > I think this thread is the longest I've seen in fifteen years on > Histonet! I find the joke a little lame, but the thread gets funnier > and funnier. > > If the joke gives offense, it's because its writer clearly has only a > very dim idea of Catholic practice (either Roman or Anglican - I'm an > Episcopalian). It would be lot funnier if these details were > rewritten. > > Lesley Weston then tried to tell a comparable story from Jewish > history, only to have it intercepted by his corporate netnanny, > apparently because it contained the word J-w. I think of playwright > Herman Wouk's book about his Orthodox faith he published about 50 > years ago - "This Is My God" is I think the most profound work of > religious apologetic written in the 20th century - well, maybe C.S. > Lewis's "Mere Christianity" - Wouk the playwright wryly observed that > the word J-w uttered on the stage is always a shocker. > > As an aside, when you forward anything on the Web, make sure always to > delete all the accumulated e-mail addresses in it - they're an > invitation to spammers. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM <@t> mcclainlab.com Tue Mar 27 16:39:01 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Tue Mar 27 16:39:18 2012 Subject: [Histonet] Avidin Red for Mast Cells fading Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF083BDB08@ML1.McClainLabs.local> Avidin Red or Avidin bound to Texas Red produces a beautiful specific stain for mast cell granule heparin. Triple stain (RGB) IF can be done by combining with fluoroscein labeled ab and a Hoescht stain for nuclei. However, the Avidin staining dissipates within days to weeks. Any suggestions? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From one_angel_secret <@t> yahoo.com Tue Mar 27 16:40:37 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 27 16:40:46 2012 Subject: [Histonet] CLIA requirements In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FB9922@SMCMAIL01.somerset-healthcare.com> References: <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> <5A33C952BB67F4468AF1F36D739212BC116309B8@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711FB9922@SMCMAIL01.somerset-healthcare.com> Message-ID: I've always kept an additional copy for my own files when inspections occur Not every place allows this btw But transcripts of education copies of state license is always handy in a inspection so I do. Under lock and key. Because personnel files should be private. Sent from my iPhone On Mar 27, 2012, at 4:27 PM, "Rathborne, Toni" wrote: > We keep all of ours in the lab. Much easier than having HR search their files for the documentation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, March 27, 2012 4:11 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] CLIA requirements > > About the personnel files, do you keep these in the lab on each employee plus what HR has or do you just call HR when CLIA arrives and let HR handle all the personnel questions? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org > Sent: Tuesday, March 27, 2012 10:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CLIA requirements > > > We ask out techs to bring in their transcripts, certifications ETC.. (they don't mind) and put a copy with their job descriptions so we have documentation when going thru inspections. > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 > > > -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- > To: Amber McKenzie > From: Victoria Baker > Sent by: histonet-bounces@lists.utsouthwestern.edu > Date: 03/27/2012 10:13AM > Cc: "histonet@lists.utsouthwestern.edu" > Subject: Re: [Histonet] CLIA requirements > > > Hi Amber, > > Try contacting HR and the Compliance Officer (which may be your lab > manager) as to how this requirement is handled. Institutions vary on this, > but most times all employee files had to be with HR. Education, CEU's, > certifications, licenses and transcripts, along with evaluations or > disiplinary actions were all based there and nothing was to be taken out or > copied. I've been at a few places recently where accessing of staff files > was possible through the intranet - but only certain levels of supervision > were able to actually access a part or all of them. > > Hope that this helps some. > > Vikki > > > > > > On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < > amber.mckenzie@gastrodocs.net> wrote: > >> >> CLIA requires me to keep a personnel file on everyone that works in the >> lab and I was wondering how you approach the staff about turning in their >> highest level of education. Do they bring in a copy of their HS diploma? >> College diploma? And what if they're not finished with college, do they >> only turn in their HS credit? Plus, copies of their certifications? >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Mar 27 16:41:54 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 27 16:43:03 2012 Subject: [Histonet] CLIA requirements In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC11630A02@JERRY.Gia.com> References: <1332836070.47360.YahooMailNeo@web114503.mail.gq1.yahoo.com> <1332853636.12838.YahooMailClassic@web162103.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BC11630731@JERRY.Gia.com> <5A33C952BB67F4468AF1F36D739212BC116309B8@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711FB9922@SMCMAIL01.somerset-healthcare.com> <5A33C952BB67F4468AF1F36D739212BC11630A02@JERRY.Gia.com> Message-ID: CLIA does state that personnel performing high complexity testing have it. So yes. They have to provide it. Sent from my iPhone On Mar 27, 2012, at 5:00 PM, Amber McKenzie wrote: > Do you make it a requirement to work in the lab that everyone provide proof of education b/c it's not a company policy where I work. Plus, does CLIA state in their website about what they require in the personnel files? Is it just employee start date, highest level of education, evals and proficiency testing? > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Tuesday, March 27, 2012 3:27 PM > To: Amber McKenzie; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] CLIA requirements > > We keep all of ours in the lab. Much easier than having HR search their files for the documentation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, March 27, 2012 4:11 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] CLIA requirements > > About the personnel files, do you keep these in the lab on each employee plus what HR has or do you just call HR when CLIA arrives and let HR handle all the personnel questions? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org > Sent: Tuesday, March 27, 2012 10:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CLIA requirements > > > We ask out techs to bring in their transcripts, certifications ETC.. (they don't mind) and put a copy with their job descriptions so we have documentation when going thru inspections. > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 > > > -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- > To: Amber McKenzie > From: Victoria Baker > Sent by: histonet-bounces@lists.utsouthwestern.edu > Date: 03/27/2012 10:13AM > Cc: "histonet@lists.utsouthwestern.edu" > Subject: Re: [Histonet] CLIA requirements > > > Hi Amber, > > Try contacting HR and the Compliance Officer (which may be your lab > manager) as to how this requirement is handled. Institutions vary on this, > but most times all employee files had to be with HR. Education, CEU's, > certifications, licenses and transcripts, along with evaluations or > disiplinary actions were all based there and nothing was to be taken out or > copied. I've been at a few places recently where accessing of staff files > was possible through the intranet - but only certain levels of supervision > were able to actually access a part or all of them. > > Hope that this helps some. > > Vikki > > > > > > On Tue, Mar 27, 2012 at 9:30 AM, Amber McKenzie < > amber.mckenzie@gastrodocs.net> wrote: > >> >> CLIA requires me to keep a personnel file on everyone that works in the >> lab and I was wondering how you approach the staff about turning in their >> highest level of education. Do they bring in a copy of their HS diploma? >> College diploma? And what if they're not finished with college, do they >> only turn in their HS credit? Plus, copies of their certifications? >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Mar 27 17:44:40 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Mar 27 17:44:42 2012 Subject: [Histonet] An immunologist... In-Reply-To: References: Message-ID: Should I not send stuff like this, then? It's safe for work. Not for puns. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Tue, Mar 27, 2012 at 4:45 PM, Eric Hoy wrote: > An immunologist, a histologist, and a microbiologist go into a bar.... > > I guess I?d better not finish this, because the political-correctness > police > will come after me. > > Anyway, it made me laugh, and I?ve worked in all three of those > professions. > > > Life is wonderful... why do so many people here need to get one? > > Eric Hoy > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood <@t> health.nsw.gov.au Tue Mar 27 18:21:54 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Mar 27 18:22:06 2012 Subject: [Histonet] RE: An immunologist... In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A24951@xmdb02.nch.kids> Well, don't leave me hanging - what happened. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Hoy Sent: Wednesday, 28 March 2012 7:46 AM To: Histonet Subject: [Histonet] An immunologist... An immunologist, a histologist, and a microbiologist go into a bar.... I guess I?d better not finish this, because the political-correctness police will come after me. Anyway, it made me laugh, and I?ve worked in all three of those professions. Life is wonderful... why do so many people here need to get one? Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Laurie <@t> blufrogpath.com Tue Mar 27 18:26:15 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Tue Mar 27 18:26:21 2012 Subject: [Histonet] Chemical Fume Hoods Message-ID: <20120327162615.295dc6182df7e5cbb4f32bc101c30dcc.5a325d2ced.wbe@email15.secureserver.net> Can anyone expand on the following CAP checklist question: < GEN.71450 Phase II There is a function verification prog hoods required by the laboratory's Chemical Hygiene P Evidence of Compliance: ? verification[DEL: :DEL] AND< [DEL: ? :DEL] Records of function verification What do they mean by "function verification?" Does this m lab personnel just have to do a function test and make sure the ho is working, or does an outside company have to test the hood for correct outside compa Laurie Colbert From tony.henwood <@t> health.nsw.gov.au Tue Mar 27 18:28:07 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Mar 27 18:28:29 2012 Subject: [Histonet] Re: Redneck Lent In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A24984@xmdb02.nch.kids> Roman or Anglican or Episcopalian? Well I'm Australian!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, 28 March 2012 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Redneck Lent I think this thread is the longest I've seen in fifteen years on Histonet! I find the joke a little lame, but the thread gets funnier and funnier. If the joke gives offense, it's because its writer clearly has only a very dim idea of Catholic practice (either Roman or Anglican - I'm an Episcopalian). It would be lot funnier if these details were rewritten. Lesley Weston then tried to tell a comparable story from Jewish history, only to have it intercepted by his corporate netnanny, apparently because it contained the word J-w. I think of playwright Herman Wouk's book about his Orthodox faith he published about 50 years ago - "This Is My God" is I think the most profound work of religious apologetic written in the 20th century - well, maybe C.S. Lewis's "Mere Christianity" - Wouk the playwright wryly observed that the word J-w uttered on the stage is always a shocker. As an aside, when you forward anything on the Web, make sure always to delete all the accumulated e-mail addresses in it - they're an invitation to spammers. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From matthew.ibbs <@t> wco.pl Wed Mar 28 01:39:59 2012 From: matthew.ibbs <@t> wco.pl (MRI) Date: Wed Mar 28 01:40:07 2012 Subject: [Histonet] Hard water. Message-ID: <4F72B23F.4070801@wco.pl> Dear all, In theory, we should all be using distilled or deionised water for making our formalin (assuming we don't buy it pre-prepared). However, I know that some labs in my area are using tap water. The water in our area is very hard ( >4.5 mol/m3 25?d) though it is pH neutral, and I'm curious if anyone knows what effects this may have on immuno and in-situ. In our lab, we buffer our formalin according to the method in "Theory and Practice" by Bancroft and Gamble so I'm assuming others in the area do too... but I could be wrong, and I'm too frequently an optimist. Should the buffers counteract negative effects from the minerals in the water or will they exacerbate the situation and add to the hardness and reduce the pH level? Any ideas? Thanks in advance, Matthew. From Susan.Walzer <@t> HCAHealthcare.com Wed Mar 28 02:26:46 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Mar 28 02:27:52 2012 Subject: [Histonet] Re: Redneck Lent In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A24984@xmdb02.nch.kids> References: <6D6BD1DE8A5571489398B392A38A715760A24984@xmdb02.nch.kids> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DE75D7302@FWDCWPMSGCMS09.hca.corpad.net> I'm Frisbeetarian, we believe when you die your soul goes up on the roof and you can't get it down. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Tuesday, March 27, 2012 7:28 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Redneck Lent Roman or Anglican or Episcopalian? Well I'm Australian!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, 28 March 2012 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Redneck Lent I think this thread is the longest I've seen in fifteen years on Histonet! I find the joke a little lame, but the thread gets funnier and funnier. If the joke gives offense, it's because its writer clearly has only a very dim idea of Catholic practice (either Roman or Anglican - I'm an Episcopalian). It would be lot funnier if these details were rewritten. Lesley Weston then tried to tell a comparable story from Jewish history, only to have it intercepted by his corporate netnanny, apparently because it contained the word J-w. I think of playwright Herman Wouk's book about his Orthodox faith he published about 50 years ago - "This Is My God" is I think the most profound work of religious apologetic written in the 20th century - well, maybe C.S. Lewis's "Mere Christianity" - Wouk the playwright wryly observed that the word J-w uttered on the stage is always a shocker. As an aside, when you forward anything on the Web, make sure always to delete all the accumulated e-mail addresses in it - they're an invitation to spammers. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shehnazster <@t> gmail.com Wed Mar 28 03:51:46 2012 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Wed Mar 28 03:51:53 2012 Subject: [Histonet] GLASSWARE CALIBRATION Message-ID: Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn From louise.renton <@t> gmail.com Wed Mar 28 04:17:17 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed Mar 28 04:20:59 2012 Subject: [Histonet] GLASSWARE CALIBRATION In-Reply-To: References: Message-ID: 1ml of water =1g mass. could you use this to determine the degree of error between calibration and "real" volume? On Wed, Mar 28, 2012 at 10:51 AM, shehnaz khan wrote: > Hi netters, > > Could someone please shed some light: > > 1. How is calibration for glassware performed on non-Class A glassware? > > 2. If Class A glassware is used but no certificate is located - does > it still require calibration? > > Thanx again. > > S Kahn > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From jqb7 <@t> cdc.gov Wed Mar 28 05:06:59 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 28 05:07:18 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: <5007488247871463800@unknownmsgid> References: <5007488247871463800@unknownmsgid> Message-ID: But hilarious! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Monday, March 26, 2012 7:56 PM To: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: RE: [Histonet] FW: Redneck Lent Religious humor on this listserv is remarkably inappropriate. I cannot believe anyone would post this here. Tasteless. Sent from my Windows Phone From: JOSEPH FRAZEE Sent: 3/26/2012 3:49 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeringk@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; yvette.fetterly@basf.com; footchina@yahoo.com; frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com Kerri ----- Forwarded Message ----- From: Sharen Pray To: Ruth Posey ; LueAnn Root ; Marjorie Norris ; "Tom Voss, Sr." ; Taber Stewart ; MONTIE L WINTERS ; Terry Maloney ; kerri spoering ; "Kenny & Debbie Hager" Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up his outdoor grill and cook a venison steak. But, all of Bubba's neighbors were Catholic. And since it was Lent, they were forbidden from eating meat on Friday. The delicious aroma from the grilled venison steaks was causing such a problem for the Catholic faithful that they finally talked to their priest. The Priest came to visit Bubba, and suggested that he become a Catholic. After several classes and much study, Bubba attended Mass...and as the priest sprinkled holy water over him, he said, "You were born a Baptist, and raised a Baptist, but now you are a Catholic." Bubba's neighbors were greatly relieved, until Friday night arrived, and the wonderful aroma of grilled venison filled the neighborhood. The Priest was called immediately by the neighbors, and, as he rushed into Bubba's yard, clutching a rosary and prepared to scold him, he stopped and watched in amazement. There stood Bubba, clutching a small bottle of holy water which he carefully sprinkled over the grilling meat and chanted: "You wuz born a deer, you wuz raised a deer, but now you is a catfish." Blessings, love and light, "Live simply, love generously, care deeply, speak kindly." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 28 05:09:29 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 28 05:09:36 2012 Subject: FW: [Histonet] FW: Redneck Lent References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> Message-ID: Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, March 28, 2012 6:09 AM To: 'Davide Costanzo' Subject: RE: [Histonet] FW: Redneck Lent Actually, I thought the joke was mostly aimed at stupid, Southern Baptists. And by the way, I am from Georgia and am Baptist. I was not offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M wrote: > For goodness sakes! It is a joke. First of all it was accidently > sent to HistoNet per Joseph's second email. But most of all can't we > just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. > has had jokes made about it. Give the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional > Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l > PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE > [ jfray80@hotmail.com] > Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; > yvette.fetterly@basf.com; footchina@yahoo.com; > frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root > ; Marjorie Norris ; "Tom > Voss, Sr." < tomvoss@wildblue.net>; Taber Stewart > ; MONTIE L WINTERS ; Terry > Maloney ; kerri spoering > ; "Kenny & Debbie Hager" < kanddhager@att.net> > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill > and cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the > priest sprinkled holy water over him, he said, "You were born a > Baptist, and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, > and the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed > into Bubba's yard, clutching a rosary and prepared to scold him, he > stopped and watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, > speak kindly." > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------------- > ---- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your > computer. Do not deliver, distribute or copy this message and do not > disclose its contents or take any action in reliance on the > information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 28 06:01:41 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Mar 28 06:04:16 2012 Subject: [Histonet] GLASSWARE CALIBRATION In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9179C9F73B5@UTHCMS1.uthouston.edu> Thank you for posting something that has to do with the business of Histotechnology rather than redneck jokes, my delete finger has blisters on it. . Let us have some meaningful discussions on the problems and solutions in today's histotechnology field. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shehnaz khan [shehnazster@gmail.com] Sent: Wednesday, March 28, 2012 3:51 AM To: histonet; histonet Subject: [Histonet] GLASSWARE CALIBRATION Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 28 07:08:59 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 28 07:09:02 2012 Subject: [Histonet] Chemical Fume Hoods In-Reply-To: <20120327162615.295dc6182df7e5cbb4f32bc101c30dcc.5a325d2ced.wbe@email15.secureserver.net> Message-ID: <1332936539.30089.YahooMailClassic@web162101.mail.bf1.yahoo.com> When you have a fume hood in your lab you should have it tested, at least once a year, by somebody that can?quantify the air flow at the hood's entrance. I used to do that and I also had at the entrance (my lab?had 5 fume hoods) I installed a small plastic air vane. The vane moved across a calibrated scale indicating the feet/minute of the air flow. We checked the vanes daily and recorded the readings in the scale. Those records, along with the more precise annual measurements, were part of our CAP inspection check list. Those vanes are not expensive and you can install them easily. Ren? J.?? --- On Tue, 3/27/12, Laurie@blufrogpath.com wrote: From: Laurie@blufrogpath.com Subject: [Histonet] Chemical Fume Hoods To: "Histonet post" Date: Tuesday, March 27, 2012, 7:26 PM ???Can anyone expand on the following CAP checklist question: <= /div> ??? ??? = ???GEN.71450 ??? ???= Fume Hood Verification??? ???Phase II ??? ??? ??? ? There? is? a function verification prog= ram for chemical fume ???hoods required by the laboratory's Chemical Hygiene P= lan. ??? ???Evidence of Compliance: ????=? ? ? ???Written? procedure? for chemical fume hood function ???verification[DEL:???:DEL] AND<= /FONT> ??? ???[DEL: ?? ? ? :DEL] Records of function verification ??? ??? ??? ???What? do? they mean by "function verification?"? Does this m= ean that ???lab personnel just have to do a function test and make sure the ho= od ???is? working,? or? does? an? outside? company have to test the hood for ???correct=? airflow? and? then? certify? it????At my last job, we had an ???outside compa= ny come out annually to test and certify the hoods. ??? ? ? ???Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 28 07:23:33 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 28 07:23:41 2012 Subject: [Histonet] Hard water. In-Reply-To: <4F72B23F.4070801@wco.pl> Message-ID: <1332937413.82712.YahooMailClassic@web162102.mail.bf1.yahoo.com> First of all I want to strongly encourage you to talk with your manager to try to eliminate the preparation of NBF in house. Formalin, as you perfectly know, is an extremely dangerous substance and any extra contact with it should be avoided. Spending some extra money in buying pre-filled vials with NBF is always worth it, because there is no price to your health. Regarding your question you are adding your chemicals and determining the pH of your solution. NBF is used to fix the tissues and?not to be used in a subsequent chemical analysis where all the components need to be known. This means that the purity of the water to prepare NBF is of little consequence in fixation,?unless you are going to use the fixed tissues to determine some types of metals, in which case you may be incorporating those?metals from the water into the tissues. For IHC or FISH you are measuring organic components and I know of no interference with water minerals during fixation. In any event, when you are adding your neutralizing chemicals and reach the desired pH that pH value is including any chemicals in the tap water and their contribution, if any. Again, the ideal situation is to buy pre-filled vials with NBF. Do you in your lab determine formalin levels in the air and on the personnel? Ren? J. ? ? ? ? --- On Wed, 3/28/12, MRI wrote: From: MRI Subject: [Histonet] Hard water. To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 28, 2012, 2:39 AM Dear all, In theory, we should all be using distilled or deionised water for making our formalin (assuming we don't buy it pre-prepared).? However, I know that some labs in my area are using tap water.? The water in our area is very hard ( >4.5 mol/m3 25?d) though it is pH neutral, and I'm curious if anyone knows what effects this may have on immuno and in-situ. In our lab, we buffer our formalin according to the method in "Theory and Practice" by Bancroft and Gamble so I'm assuming others in the area do too... but I could be wrong, and I'm too frequently an optimist. Should the buffers counteract negative effects from the minerals in the water or will they exacerbate the situation and add to the hardness and reduce the pH level? Any ideas? Thanks in advance, Matthew. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 28 07:38:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 28 07:39:00 2012 Subject: [Histonet] GLASSWARE CALIBRATION In-Reply-To: Message-ID: <1332938331.41907.YahooMailClassic@web162105.mail.bf1.yahoo.com> Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ? 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20?C and I do not think that 20?C is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment),?a good thermometer to determine the?distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result?(in grams) between the density of the water at the temperature you made the measurement and you will get the value?of the volume. Too much trouble, just trust the manufacturer of your glassware! Ren? J.? --- On Wed, 3/28/12, shehnaz khan wrote: From: shehnaz khan Subject: [Histonet] GLASSWARE CALIBRATION To: "histonet" , "histonet" Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2.? If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> dsmanitoba.ca Wed Mar 28 08:46:32 2012 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Wed Mar 28 08:55:24 2012 Subject: [Histonet] Phosphorylase method Message-ID: Hi, Does anyone have a good enzyme method for Phosphorylase done on muscle bx's. We have been doing the method from Dubowitz, 2nd edition for years but would like to get away from the messy PVP & coverslipping. We are trying cytoseal, which seems to work fine for coverslipping, but would like to compare other methods without PVP. Also, does anyone know the purpose of PVP, glyceron or dextrin in this test? Thanks for any help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From Lynn.Burton <@t> Illinois.gov Wed Mar 28 09:20:45 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Mar 28 09:21:13 2012 Subject: [Histonet] FW: Redneck Lent In-Reply-To: References: <1234431471.204582.1332205030955.JavaMail.root@md14.quartz.synacor.com> <1332641192.63488.YahooMailNeo@web112810.mail.gq1.yahoo.com> <1332788127.23142.YahooMailNeo@web24918.mail.ird.yahoo.com> <7EAFE982E328304DA6CE2B677BB7624601C9805A@TN001WEXMBX12.US.chs.net> , Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00649C40B37@IL084EXMBX214.illinois.gov> Let's move on please. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) [jqb7@cdc.gov] Sent: Wednesday, March 28, 2012 5:09 AM To: Histonet Server Subject: FW: [Histonet] FW: Redneck Lent Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, March 28, 2012 6:09 AM To: 'Davide Costanzo' Subject: RE: [Histonet] FW: Redneck Lent Actually, I thought the joke was mostly aimed at stupid, Southern Baptists. And by the way, I am from Georgia and am Baptist. I was not offended. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike & tony siltman; Taylors Cars Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M wrote: > For goodness sakes! It is a joke. First of all it was accidently > sent to HistoNet per Joseph's second email. But most of all can't we > just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. > has had jokes made about it. Give the guy a break. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional > Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l > PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE > [ jfray80@hotmail.com] > Sent: Monday, March 26, 2012 6:48 PM > To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike & tony siltman > Subject: [Histonet] FW: Redneck Lent > > Date: Mon, 26 Mar 2012 19:55:27 +0100 > From: spoeringk@yahoo.com > Subject: Fw: Fwd: Redneck Lent > To: karen.greenlee@hobbylobby.com; stewartdaphne@hotmail.com; > yvette.fetterly@basf.com; footchina@yahoo.com; > frazeelinda@hotmail.com; jfray80@hotmail.com; donna.lueck@gmail.com > > > > > Kerri > > > > ----- Forwarded Message ----- > From: Sharen Pray > To: Ruth Posey ; LueAnn Root > ; Marjorie Norris ; "Tom > Voss, Sr." < tomvoss@wildblue.net>; Taber Stewart > ; MONTIE L WINTERS ; Terry > Maloney ; kerri spoering > ; "Kenny & Debbie Hager" < kanddhager@att.net> > Sent: Saturday, 24 March 2012, 21:06 > Subject: Fw: Fwd: Redneck Lent > > > > > > > > > > > > > > > > > > > Each Friday night after work, Bubba would fire up his outdoor grill > and cook a venison steak. But, all of > > > > > > Bubba's neighbors were Catholic. And since it was Lent, they were > forbidden from eating meat on Friday. > > > The delicious aroma from the grilled venison steaks was causing such a > problem for the Catholic faithful that they finally talked to their priest. > > The Priest came to visit Bubba, and suggested that he become a Catholic. > > After several classes and much study, Bubba attended Mass...and as the > priest sprinkled holy water over him, he said, "You were born a > Baptist, and raised a Baptist, but now you are a Catholic." > > Bubba's neighbors were greatly relieved, until Friday night arrived, > and the wonderful aroma of grilled venison filled the neighborhood. > > The Priest was called immediately by the neighbors, and, as he rushed > into Bubba's yard, clutching a rosary and prepared to scold him, he > stopped and watched in amazement. > > There stood Bubba, clutching a small bottle of holy water which he > carefully sprinkled over the grilling meat and chanted: > "You wuz born a > deer, you wuz raised a deer, but now you is a catfish." > > > > Blessings, love and light, "Live simply, love generously, care deeply, > speak kindly." > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------------- > ---- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your > computer. Do not deliver, distribute or copy this message and do not > disclose its contents or take any action in reliance on the > information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amkmadden <@t> gmail.com Wed Mar 28 09:56:13 2012 From: amkmadden <@t> gmail.com (Amanda Madden) Date: Wed Mar 28 09:56:42 2012 Subject: [Histonet] Thionin Staining Recipe and Protocol Message-ID: Good Morning Histonetters! I am looking for advice on my thionin staining recipe and protocol. The recipe I use currently ends up being just under 1% Thionin. It is made by mixing 80ml 1M acetic acid and 14.4ml 1M NAOH to a pH of 4, and then adding 305.6ml 1.3% stock thionin. When I used this protocol last, I rehydrated the mounted rat brain sections (perfused with 4% buffered para) through ETOH to dH20 steps, then left the slides in thionin for 6 minutes, followed by 2x2min rinses in dH20, 1 min in 70% ETOH, 3 min in 95% ETOH, and finally absolute ETOH and xylene for coverslipping. The tissue came out too dark, and what I am wondering is if I should change the thionin recipe to something a bit more dilute, or if I should focus on the baths following thionin, for instance only rinsing in water and moving into a .1% acetic acid in 70% ETOH differentiator? Any help, either by directing my own troubleshooting or even recipes and protocols would be greatly appreciated! Thanks, as always, for being such wonderful resources for those like myself who have such little histology background :) Amanda Madden From mcauliff <@t> umdnj.edu Wed Mar 28 10:28:14 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Mar 28 10:27:01 2012 Subject: [Histonet] Thionin Staining Recipe and Protocol In-Reply-To: References: Message-ID: <4F732E0E.1060407@umdnj.edu> Simple CV or Thionine method: Cresyl Violet or Thionine0.1 gram Distilled water100 ml when dissolved add 10% acetic acid0.5 ml *or * glacial acetic acid100 ?l 1. Bring paraffin sections to water as above, frozen or cryostat sections are rinsed in water. 2. Stain 2-10 minutes in 0.1% cresyl violet or thionine. 3. Rinse in 2 changes of distilled water, 30 seconds to one minute each. 4. Dehydrate quickly in a few dips in 70% ethanol then 95% ethanol, 15 seconds, then 2 changes of 100% ethanol, one minute each. 5. Clear in 2 changes of xylene and coverslip with DPX mounting medium. Some stain will come out in the lower alcohols which is normal. Only nuclei and Nissl should stain. Sounds like your stain is too concentrated. OR, if you prefer a buffered solution ... 1. Bring paraffin sections to water. 2. Stain 20 minutes in: 0.1% aqueous *Cresyl Violet* or *Thionin*, one part. 0.2 M Walpole acetate buffer, pH 4.05, ten parts. (16 ml 0.2M acetic acid + 4 ml 0.2M sodium acetate) 3. Rinse twice in distilled water, 30 seconds to one minute each. 4. Dehydrate quickly in 95% ethanol, 15-30 seconds, then 2 changes of 100% ethanol, one minute each. 5. Clear and coverslip. Source:_Staining Procedures_, 4th edition., George Clark editor. Geoff On 3/28/2012 10:56 AM, Amanda Madden wrote: > Good Morning Histonetters! > > I am looking for advice on my thionin staining recipe and protocol. The > recipe I use currently ends up being just under 1% Thionin. It is made by > mixing 80ml 1M acetic acid and 14.4ml 1M NAOH to a pH of 4, and then adding > 305.6ml 1.3% stock thionin. When I used this protocol last, I rehydrated > the mounted rat brain sections (perfused with 4% buffered para) through > ETOH to dH20 steps, then left the slides in thionin for 6 minutes, followed > by 2x2min rinses in dH20, 1 min in 70% ETOH, 3 min in 95% ETOH, and finally > absolute ETOH and xylene for coverslipping. The tissue came out too dark, > and what I am wondering is if I should change the thionin recipe to > something a bit more dilute, or if I should focus on the baths following > thionin, for instance only rinsing in water and moving into a .1% acetic > acid in 70% ETOH differentiator? Any help, either by directing my own > troubleshooting or even recipes and protocols would be greatly appreciated! > > Thanks, as always, for being such wonderful resources for those like myself > who have such little histology background :) > > Amanda Madden > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From CThornton <@t> dahlchase.com Wed Mar 28 10:43:29 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Mar 28 10:43:33 2012 Subject: [Histonet] Ventana XT/Ultra Message-ID: Has anyone ever worked up cost/slide for either the XT and/or Ultra as far as the bulk fluids are concerned? Does anyone know how much bulk fluid (LCS, Reaction buffer, CC1) is used per slide during a typical run? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From gu.lang <@t> gmx.at Wed Mar 28 11:04:55 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 28 11:05:05 2012 Subject: AW: [Histonet] Hard water. In-Reply-To: <4F72B23F.4070801@wco.pl> References: <4F72B23F.4070801@wco.pl> Message-ID: <002101cd0cfc$84b0e5d0$8e12b170$@gmx.at> Dear Matthew, Formaldehyd in aequoes solution undergoes the "Cannizzaro-reaction". Please google it for further info. In the end the pH is rendered acid with prolonged storage of unbuffered formalin. Then you have to deal with slightly acid formalin and that was shown to influence immunohistochemistry. And unbuffered formalin may lead to even more fragmented DNA than usually with NBF. In tapwater diluted formaldehyde was formerly the usual kind of preparation, but the acid was neutralized by putting a piece of marble into the container. The minerals in tapwater may influence the buffering-action of phosphatebuffer, but that could be easily shown by ph-measuring of the ready solution. I don't believe, that additional ions in the fixative from tapwater have any influence on the fixing action. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von MRI Gesendet: Mittwoch, 28. M?rz 2012 08:40 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Hard water. Dear all, In theory, we should all be using distilled or deionised water for making our formalin (assuming we don't buy it pre-prepared). However, I know that some labs in my area are using tap water. The water in our area is very hard ( >4.5 mol/m3 25?d) though it is pH neutral, and I'm curious if anyone knows what effects this may have on immuno and in-situ. In our lab, we buffer our formalin according to the method in "Theory and Practice" by Bancroft and Gamble so I'm assuming others in the area do too... but I could be wrong, and I'm too frequently an optimist. Should the buffers counteract negative effects from the minerals in the water or will they exacerbate the situation and add to the hardness and reduce the pH level? Any ideas? Thanks in advance, Matthew. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Mar 28 11:08:11 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Mar 28 11:08:23 2012 Subject: [Histonet] GLASSWARE CALIBRATION In-Reply-To: <1332938331.41907.YahooMailClassic@web162105.mail.bf1.yahoo.com> References: <1332938331.41907.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: Glass glassware, including glass pipets, has always been accurate enough for my needs in making up histological stains. This is not (repeat NOT) true of pipettors with disposable tips: Some of my pipettors deliver exactly what they say they do, some deliver 50% more, and some deliver 3 times as much as their factory calibration claims. I calibrate my pipettors by weighing the average volume of water delivered in 5 trials and write the "true" volume on a tape label on the barrel of the pipettor. Antibody dilutions using 2 pipettors thus require a bit of calculation. Allen A. Smith Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 28, 2012 8:39 AM To: histonet; histonet; shehnaz khan Subject: Re: [Histonet] GLASSWARE CALIBRATION Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ? 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20?C and I do not think that 20?C is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment),?a good thermometer to determine the?distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result?(in grams) between the density of the water at the temperature you made the measurement and you will get the value?of the volume. Too much trouble, just trust the manufacturer of your glassware! Ren? J.? --- On Wed, 3/28/12, shehnaz khan wrote: From: shehnaz khan Subject: [Histonet] GLASSWARE CALIBRATION To: "histonet" , "histonet" Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2.? If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> dsmanitoba.ca Wed Mar 28 11:11:41 2012 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Wed Mar 28 11:14:17 2012 Subject: [Histonet] Phosphorylase method In-Reply-To: References: Message-ID: Thanks for the quick response. I would love to take a look at your method. Our method from Dubowitz's 2nd edition has worked fine for years, but can leave residue on the slides, especially when fresh, fades & is very messy to file and we seem to be going back to previous bx's with more frequency. The 3rd edition of Dubowitz & Dawson's Neuropathology Techniques do not use PVP or glycogen, but use Dextran. This solution is much nicer to work with, not as thick & does not leave deposits on the slide when fresh and claims to appear stable for several years. So, finally, after doing this test one way "because we have always done it that way", I am trying to clean it up a little. Also, do you do phosphorylase routinely, if not what is the criteria for doing it. At present we do it as a part of a panel for all muscle bx's (which amounts to 80 to 110 per year). It seems this may not be necessary. Thanks again for your interest; I would appreciate your input. Have a good day, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -----Original Message----- From: Mitchell Jean A [mailto:JMitchell@uwhealth.org] Sent: Wednesday, March 28, 2012 9:07 AM To: Sharon Allen Subject: RE: [Histonet] Phosphorylase method Sharon: Just curious - Does the phosphorylase method you use fade after time? The method I use does use PVP and dextran (no glyceron though). I am not certain of why these compounds are used; may have something to do with carbohydrate bonding but wouldn't swear by it. I make the incubation media up in larger batches; aliquot into smaller amounts; freeze at -20 until ready to use. I section my muscle onto 22 X 22 coverslips and only use 10ml aliquots of incubation media at a time. Your name sounds familiar; possibly from one of the muscle workshops I have given at NSH? Anyway - if you don't already have my procedure - let me know and I will forward. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Wednesday, March 28, 2012 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Phosphorylase method Hi, Does anyone have a good enzyme method for Phosphorylase done on muscle bx's. We have been doing the method from Dubowitz, 2nd edition for years but would like to get away from the messy PVP & coverslipping. We are trying cytoseal, which seems to work fine for coverslipping, but would like to compare other methods without PVP. Also, does anyone know the purpose of PVP, glyceron or dextrin in this test? Thanks for any help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From Lynn.Burton <@t> Illinois.gov Wed Mar 28 11:24:53 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Mar 28 11:25:03 2012 Subject: [Histonet] RE: Ventana XT/Ultra In-Reply-To: References: Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00649C40B3D@IL084EXMBX214.illinois.gov> We have. I don't recall the amount dispensed but my notes say the cost, per slide, is as follows: LCS $.02 CC1 .28 EZ Prep $.15 Reaction Buffer $.02 Ultra View kit $5.09 My brain keeps telling me the dispensed amount is 1,uL? Tech support at Ventana is great about answering those questions. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton [CThornton@dahlchase.com] Sent: Wednesday, March 28, 2012 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana XT/Ultra Has anyone ever worked up cost/slide for either the XT and/or Ultra as far as the bulk fluids are concerned? Does anyone know how much bulk fluid (LCS, Reaction buffer, CC1) is used per slide during a typical run? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Mar 28 11:27:47 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Mar 28 11:27:51 2012 Subject: [Histonet] Ventana XT/Ultra In-Reply-To: References: Message-ID: Ventana can give you these numbers based on your contract pricing for bulk fluids. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, March 28, 2012 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana XT/Ultra Has anyone ever worked up cost/slide for either the XT and/or Ultra as far as the bulk fluids are concerned? Does anyone know how much bulk fluid (LCS, Reaction buffer, CC1) is used per slide during a typical run? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Wed Mar 28 11:37:44 2012 From: member <@t> linkedin.com (Peggy Brask via LinkedIn) Date: Wed Mar 28 11:37:47 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1603752117.18669201.1332952664307.JavaMail.app@ela4-app0128.prod> LinkedIn ------------ Peggy Brask requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Peggy Accept invitation from Peggy Brask http://www.linkedin.com/e/yvpgd1-h0cllm9b-1e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I244781281_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYNe38Ne3sQd399bRBxhlhGuAxPbPcPdz4Ve3oRejkLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=3d4U2y1h35_581 View invitation from Peggy Brask http://www.linkedin.com/e/yvpgd1-h0cllm9b-1e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I244781281_13/3cNnP4Ucz4UdPgQcAALqnpPbOYWrSlI/svi/?hs=false&tok=3xVZwBccP5_581 ------------------------------------------ Why might connecting with Peggy Brask be a good idea? Peggy Brask's connections could be useful to you: After accepting Peggy Brask's invitation, check Peggy Brask's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From JMitchell <@t> uwhealth.org Wed Mar 28 12:02:25 2012 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Wed Mar 28 12:02:32 2012 Subject: [Histonet] Phosphorylase method In-Reply-To: References: Message-ID: Sharon: we do the phosphorylase procedure routinely on all muscle biopsies. It is a stain that very, very seldom have we seen an abnormal result. But phosphorylase is also a secondary stain to review in the case of glycogen storage to confirm those findings. I will forward my phosphorylase procedure to you in a separate email. The procedure has clean results, is permanent; and can be coverslipped with regular mounting media (I use cytoseal). Jean -----Original Message----- From: Sharon Allen [mailto:SAllen@dsmanitoba.ca] Sent: Wednesday, March 28, 2012 11:12 AM To: Mitchell Jean A Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Phosphorylase method Thanks for the quick response. I would love to take a look at your method. Our method from Dubowitz's 2nd edition has worked fine for years, but can leave residue on the slides, especially when fresh, fades & is very messy to file and we seem to be going back to previous bx's with more frequency. The 3rd edition of Dubowitz & Dawson's Neuropathology Techniques do not use PVP or glycogen, but use Dextran. This solution is much nicer to work with, not as thick & does not leave deposits on the slide when fresh and claims to appear stable for several years. So, finally, after doing this test one way "because we have always done it that way", I am trying to clean it up a little. Also, do you do phosphorylase routinely, if not what is the criteria for doing it. At present we do it as a part of a panel for all muscle bx's (which amounts to 80 to 110 per year). It seems this may not be necessary. Thanks again for your interest; I would appreciate your input. Have a good day, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -----Original Message----- From: Mitchell Jean A [mailto:JMitchell@uwhealth.org] Sent: Wednesday, March 28, 2012 9:07 AM To: Sharon Allen Subject: RE: [Histonet] Phosphorylase method Sharon: Just curious - Does the phosphorylase method you use fade after time? The method I use does use PVP and dextran (no glyceron though). I am not certain of why these compounds are used; may have something to do with carbohydrate bonding but wouldn't swear by it. I make the incubation media up in larger batches; aliquot into smaller amounts; freeze at -20 until ready to use. I section my muscle onto 22 X 22 coverslips and only use 10ml aliquots of incubation media at a time. Your name sounds familiar; possibly from one of the muscle workshops I have given at NSH? Anyway - if you don't already have my procedure - let me know and I will forward. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Wednesday, March 28, 2012 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Phosphorylase method Hi, Does anyone have a good enzyme method for Phosphorylase done on muscle bx's. We have been doing the method from Dubowitz, 2nd edition for years but would like to get away from the messy PVP & coverslipping. We are trying cytoseal, which seems to work fine for coverslipping, but would like to compare other methods without PVP. Also, does anyone know the purpose of PVP, glyceron or dextrin in this test? Thanks for any help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca From liz <@t> premierlab.com Wed Mar 28 12:04:34 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 28 12:04:42 2012 Subject: [Histonet] GLASSWARE CALIBRATION In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5A91@SBS2K8.premierlab.local> Allen Any pipette calibration company can test and calibrate your pipettors so they are dispensing accurately and within specifications. The cost is not that significant. We have our pipettors calibrated quarterly but twice a year is good too. If you are using variable pipettors they should be calibrated at several volumes. For example a variable pipettor that ranges from 100 to 1000 ul should be measured and calibrated at 1000ul, 500ul and 100ul Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, March 28, 2012 10:08 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GLASSWARE CALIBRATION Glass glassware, including glass pipets, has always been accurate enough for my needs in making up histological stains. This is not (repeat NOT) true of pipettors with disposable tips: Some of my pipettors deliver exactly what they say they do, some deliver 50% more, and some deliver 3 times as much as their factory calibration claims. I calibrate my pipettors by weighing the average volume of water delivered in 5 trials and write the "true" volume on a tape label on the barrel of the pipettor. Antibody dilutions using 2 pipettors thus require a bit of calculation. Allen A. Smith Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 28, 2012 8:39 AM To: histonet; histonet; shehnaz khan Subject: Re: [Histonet] GLASSWARE CALIBRATION Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ? 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20?C and I do not think that 20?C is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment), a good thermometer to determine the distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result (in grams) between the density of the water at the temperature you made the measurement and you will get the value of the volume. Too much trouble, just trust the manufacturer of your glassware! Ren? J. --- On Wed, 3/28/12, shehnaz khan wrote: From: shehnaz khan Subject: [Histonet] GLASSWARE CALIBRATION To: "histonet" , "histonet" Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Mar 28 12:18:29 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Mar 28 12:18:37 2012 Subject: [Histonet] "placenta encapsulation" Message-ID: Just when you thought life couldn't get weirder, it does. I've been hearing scattered tales of women eating their own (actually their babies') placentas for years, but I didn't know about "placenta encapsulation" until my daughter's college classmate Nancy Redd (she's at nancyredd.com) bylined a New York Times story http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placenta/?scp=1&sq=placenta&st=cse and then got on ABC news this morning. http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 It seems the pill-'n'-potion culture has come to the rescue, with services (they seem to be local operations, no mail-order, probably how they slip under the regulatory radar) that for $200 or so will dry and grind up your baby's placenta and put it into large gelatin capsules. It has to be your own baby's placenta, by the way. Here's a graphic demonstration of how it's done http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Instructions_w_Pictures And here's a FAQ that answers some, but not all questions a pathology service might have. http://birth-wise.org/faq They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. An obvious concern would be bacterial overgrowth. Eeeeewwww! Bob Richmond Samurai Pathologist Knoxville TN From kkienitz <@t> orclinic.com Wed Mar 28 12:23:58 2012 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Wed Mar 28 12:27:59 2012 Subject: [Histonet] RE: Ventana XT/Ultra In-Reply-To: References: Message-ID: <41400FFE517878449D89114DD25260900848688BD3@tocmail1.tocad.orclinic.com> Be careful assuming costs. The cost of all consumables through Ventana is dependent of your instituitions volume and contractual agreement. The only way to have an accurate accounting is for Ventana to do a quote or go by list pricing. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kbradshaw@orclinic.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton [CThornton@dahlchase.com] Sent: Wednesday, March 28, 2012 8:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana XT/Ultra Has anyone ever worked up cost/slide for either the XT and/or Ultra as far as the bulk fluids are concerned? Does anyone know how much bulk fluid (LCS, Reaction buffer, CC1) is used per slide during a typical run? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Mar 28 12:32:53 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Mar 28 12:33:01 2012 Subject: [Histonet] "placenta encapsulation" In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF93954D58EE@chimsx08.CHI.catholichealth.net> Now THAT"S NATURAL CHILDBIRTH! In my 30 years of histology, I have had two such requests. (Interestingly, one was about ten years ago - must have been an early adopter) Risk management has a much bigger headache than I do, I just deliver it to their department, they get it to the patient. Question for histonetters - Is this cannibalism? One of the questions I was never allowed to ask at an interview "What is your feeling about cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, I do not advocate cannibalism, and I am sorry if I offeneded any cannibals (in the vein of redneck lent thread) - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, March 28, 2012 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "placenta encapsulation" Just when you thought life couldn't get weirder, it does. I've been hearing scattered tales of women eating their own (actually their babies') placentas for years, but I didn't know about "placenta encapsulation" until my daughter's college classmate Nancy Redd (she's at nancyredd.com) bylined a New York Times story http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent a/?scp=1&sq=placenta&st=cse and then got on ABC news this morning. http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 It seems the pill-'n'-potion culture has come to the rescue, with services (they seem to be local operations, no mail-order, probably how they slip under the regulatory radar) that for $200 or so will dry and grind up your baby's placenta and put it into large gelatin capsules. It has to be your own baby's placenta, by the way. Here's a graphic demonstration of how it's done http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst ructions_w_Pictures And here's a FAQ that answers some, but not all questions a pathology service might have. http://birth-wise.org/faq They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. An obvious concern would be bacterial overgrowth. Eeeeewwww! Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From billodonnell <@t> catholichealth.net Wed Mar 28 12:40:35 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Mar 28 12:40:42 2012 Subject: [Histonet] "placenta encapsulation" In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93954D58EE@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF93954D58EE@chimsx08.CHI.catholichealth.net> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93954D58F2@chimsx08.CHI.catholichealth.net> OK, this is too funny. I swear I got this message concerning my last post. I wonder what triggered it? Are ca**ibals now a protected minori*y? "This email has violated the R-AC-IAL DIS-CRIMI_NATION. and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. Message details: Server: BCHEXEG Sender: (deleted) Recipient: rsrichmond@gmail.com;histonet@lists.utsouthwestern.edu; Subject: RE: [Histonet] "placenta encapsulation" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, March 28, 2012 12:33 PM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] "placenta encapsulation" Now THAT"S NATURAL CHILDBIRTH! In my 30 years of histology, I have had two such requests. (Interestingly, one was about ten years ago - must have been an early adopter) Risk management has a much bigger headache than I do, I just deliver it to their department, they get it to the patient. Question for histonetters - Is this cannibalism? One of the questions I was never allowed to ask at an interview "What is your feeling about cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, I do not advocate cannibalism, and I am sorry if I offeneded any cannibals (in the vein of redneck lent thread) - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, March 28, 2012 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "placenta encapsulation" Just when you thought life couldn't get weirder, it does. I've been hearing scattered tales of women eating their own (actually their babies') placentas for years, but I didn't know about "placenta encapsulation" until my daughter's college classmate Nancy Redd (she's at nancyredd.com) bylined a New York Times story http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent a/?scp=1&sq=placenta&st=cse and then got on ABC news this morning. http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 It seems the pill-'n'-potion culture has come to the rescue, with services (they seem to be local operations, no mail-order, probably how they slip under the regulatory radar) that for $200 or so will dry and grind up your baby's placenta and put it into large gelatin capsules. It has to be your own baby's placenta, by the way. Here's a graphic demonstration of how it's done http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst ructions_w_Pictures And here's a FAQ that answers some, but not all questions a pathology service might have. http://birth-wise.org/faq They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. An obvious concern would be bacterial overgrowth. Eeeeewwww! Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From jqb7 <@t> cdc.gov Wed Mar 28 12:41:50 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 28 12:42:55 2012 Subject: [Histonet] "placenta encapsulation" In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93954D58F2@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF93954D58EE@chimsx08.CHI.catholichealth.net> <4940DF6D1C5FDF48931B6966AAEF93954D58F2@chimsx08.CHI.catholichealth.net> Message-ID: I thought it was an early April Fool's Day joke...... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, March 28, 2012 1:41 PM To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] "placenta encapsulation" OK, this is too funny. I swear I got this message concerning my last post. I wonder what triggered it? Are ca**ibals now a protected minori*y? "This email has violated the R-AC-IAL DIS-CRIMI_NATION. and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. Message details: Server: BCHEXEG Sender: (deleted) Recipient: rsrichmond@gmail.com;histonet@lists.utsouthwestern.edu; Subject: RE: [Histonet] "placenta encapsulation" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, March 28, 2012 12:33 PM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] "placenta encapsulation" Now THAT"S NATURAL CHILDBIRTH! In my 30 years of histology, I have had two such requests. (Interestingly, one was about ten years ago - must have been an early adopter) Risk management has a much bigger headache than I do, I just deliver it to their department, they get it to the patient. Question for histonetters - Is this cannibalism? One of the questions I was never allowed to ask at an interview "What is your feeling about cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, I do not advocate cannibalism, and I am sorry if I offeneded any cannibals (in the vein of redneck lent thread) - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, March 28, 2012 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "placenta encapsulation" Just when you thought life couldn't get weirder, it does. I've been hearing scattered tales of women eating their own (actually their babies') placentas for years, but I didn't know about "placenta encapsulation" until my daughter's college classmate Nancy Redd (she's at nancyredd.com) bylined a New York Times story http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent a/?scp=1&sq=placenta&st=cse and then got on ABC news this morning. http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 It seems the pill-'n'-potion culture has come to the rescue, with services (they seem to be local operations, no mail-order, probably how they slip under the regulatory radar) that for $200 or so will dry and grind up your baby's placenta and put it into large gelatin capsules. It has to be your own baby's placenta, by the way. Here's a graphic demonstration of how it's done http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst ructions_w_Pictures And here's a FAQ that answers some, but not all questions a pathology service might have. http://birth-wise.org/faq They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. An obvious concern would be bacterial overgrowth. Eeeeewwww! Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail2 <@t> gmail.com Wed Mar 28 13:08:37 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Wed Mar 28 13:08:44 2012 Subject: [Histonet] "placenta encapsulation" In-Reply-To: References: Message-ID: Too Too gross. A veritable bacterial breeding ground. Rena Fail On Wed, Mar 28, 2012 at 1:18 PM, Bob Richmond wrote: > Just when you thought life couldn't get weirder, it does. > > I've been hearing scattered tales of women eating their own (actually > their babies') placentas for years, but I didn't know about "placenta > encapsulation" until my daughter's college classmate Nancy Redd (she's > at nancyredd.com) bylined a New York Times story > > http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placenta/?scp=1&sq=placenta&st=cse > > and then got on ABC news this morning. > http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 > > It seems the pill-'n'-potion culture has come to the rescue, with > services (they seem to be local operations, no mail-order, probably > how they slip under the regulatory radar) that for $200 or so will dry > and grind up your baby's placenta and put it into large gelatin > capsules. It has to be your own baby's placenta, by the way. Here's a > graphic demonstration of how it's done > > http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Instructions_w_Pictures > > And here's a FAQ that answers some, but not all questions a pathology > service might have. > http://birth-wise.org/faq > > They want the placenta refrigerated, or possibly frozen. Formaldehyde > is prohibited. > > Seems to me that this could turn into a major headache for a pathology > service, and that some conferring with the necessary people in advance > would be a good idea. > > An obvious concern would be bacterial overgrowth. > > Eeeeewwww! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From matthew.ibbs <@t> wco.pl Wed Mar 28 13:16:53 2012 From: matthew.ibbs <@t> wco.pl (Matthew Ibbs) Date: Wed Mar 28 13:17:05 2012 Subject: [Histonet] Hard water Message-ID: <4F735595.7030604@wco.pl> Thanks for the replies to my question regarding minerals in "hard" tap water used in the production of formalin. I'm glad there's no known issue with interactions between the minerals and the fixation process. That clears up a few points that we've been asked about recently. Ren?: Regular testing of air quality around personnel and in general in lab areas is required by Polish law, and is carried out by the local sanitation and epidemiology laboratory. Exposure to formaldehyde is pretty well controlled but the purchase of ready made NBF is almost unheard of - mostly owing to costs. The Polish health service is not particularly well off and NBF costs around 10 times more than the raw materials needed to make it in house. Gudrun: Thanks for your input. At least in the case of our laboratory, formalin is used rapidly, around 50 litres per day. We buffer our formalin and the pH of our water, buffers (pre-mixed) and final formalin solution are all 7. No problems here then. The smaller labs in our area though, may or may not buffer their formalin and some of them do very little work and so may be storing formalin for extended periods. That being the case, the DNA fragmentation you mentioned would certainly interfere with ISH, though possibly less so with immunohistochemistry. This nicely lines up with what we've been observing locally. In house IHC and ISH work OK. Material referred to us from outside often works with IHC but ISH (usually FISH) results in very weakly fluorescent signals. Thanks again for the replies. Matthew. From shehnazster <@t> gmail.com Wed Mar 28 13:58:39 2012 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Wed Mar 28 13:58:42 2012 Subject: [Histonet] Re: GLASSWARE CALIBRATION In-Reply-To: References: Message-ID: Hi , Thank you all for an enlightening response. Best regards S.Kahn On Wed, Mar 28, 2012 at 11:51 AM, shehnaz khan wrote: > Hi netters, > > Could someone please shed some light: > > 1. How is calibration for glassware performed on non-Class A glassware? > > 2. If Class A glassware is used but no certificate is located - does > it still require calibration? > > Thanx again. > > S Kahn > From VHammel <@t> primecare.org Wed Mar 28 14:48:05 2012 From: VHammel <@t> primecare.org (Hammel, Vicky) Date: Wed Mar 28 14:51:53 2012 Subject: [Histonet] (no subject) Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C6938B5A@EXCHANGE2K7.staprimecare.org> I am looking to find a control block for EBV. I have blocks I can trade (melanoma or H. pylori). Please contact me if anyone is interested. Thank you Vicky Hammel HTL, ASCP Pathology Technical Consultant vhammel@primecare.org St. Alexius Medical Center Histology Laboratory 900 east Broadway Bismarck, ND 58506 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From mehlikafaire <@t> hotmail.com Wed Mar 28 17:13:17 2012 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Wed Mar 28 17:13:21 2012 Subject: [Histonet] pJNK Anitbody Message-ID: Hello, Does anyone recommend a good pJNK antibody for frozen sections? These sections have been fixed in 4% PFA overnight. If you know of any, or a reliable antigen retrieval protocol for frozen sections, that would be helpful. Thanks, Mehlika From tony.henwood <@t> health.nsw.gov.au Wed Mar 28 17:17:48 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Mar 28 17:18:02 2012 Subject: [Histonet] RE: Phosphorylase method In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A24FBC@xmdb02.nch.kids> This is the method we use: Phosphorylase Use McArdle's disease (myophosphorylase deficiency) is the most common glycogenosis affecting skeletal muscle. The biochemical hallmark of the disease is impaired muscle glycogenolysis due to lack of muscle glycogen phosphorylase (M-GP) activity. M-GP is the muscle-specific isoform of the enzyme, with two others being the liver- and the brain-specific forms. Muscle biopsies of McArdle's patients are characterized by subsarcolemmal storage of normal glycogen and by absent stain with the histochemical phosphorylase reaction. In some patients, however, it is possible to find scattered fibers with positive phosphorylase staining. Underlying Principle Phosphorylase catalyses the breakdown of Glucose-1-phosphate to glucose and phosphate ions. The Glucose is subsequently stained with iodine to give a purple localisation. Fixation and Sectioning Air dried unfixed 8?M cryostat sections Reagents 1. 0.2M Sodium Acetate Sodium acetate 3.6g Distilled water 500ml 2. 0.2M Acetic Acid Glacial acetic acid( 17N) 5.9ml Distilled water make up to 500ml total volume 3. 0.1M Sodium hydroxide Sodium hydroxide pellets 2.0g Distilled water 500ml 4. Incubating medium 0.2M sodium acetate 15ml 0.2M acetic acid 5ml Absolute alcohol 5ml Glucose 1 phosphate 0.015 g Adenosine monophophate (AMP) 0.015 g EDTA 0.025 g Sodium fluoride 0.020 g Dextran 1.000 g Adjust pH to 5.9 using 0.1M NaOH 5. Grams iodine Iodine 10.0g Potassium iodide 2.0g Distilled water 300ml Dissolve the Potassium Iodide in a small amount of water, dissolve the iodine in this, make up to 300ml. Method 1. Allow frozen sections to dry approx. 45min. 2. Incubate in above medium for 60min at 37?C 3. Wash slides in distilled water 4. Stain in Grams iodine 1 min, 5. Wash well in distilled water 6. Air dry 7. Clear in xylene 8. Mount in DPX Results Phosphorylase positive muscle fibres purple Deficient muscle fibres and background yellow-brown Smooth muscle in artery walls purple References Martinuzzi et al (1999) "McArdle's Disease - The Unsolved Mystery of the Reappearing Enzyme" American Journal of Pathology.;154:1893-1897 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 29 March 2012 12:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Phosphorylase method Hi, Does anyone have a good enzyme method for Phosphorylase done on muscle bx's. We have been doing the method from Dubowitz, 2nd edition for years but would like to get away from the messy PVP & coverslipping. We are trying cytoseal, which seems to work fine for coverslipping, but would like to compare other methods without PVP. Also, does anyone know the purpose of PVP, glyceron or dextrin in this test? Thanks for any help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From cmiller <@t> gladstone.ucsf.edu Wed Mar 28 20:40:39 2012 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Wed Mar 28 20:40:45 2012 Subject: [Histonet] Re: "placenta encapsulation" In-Reply-To: References: Message-ID: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> Well....I am 6 months pregnant, and was planning on taking a pot of formalin and a few blades into the delivery suite to get some of my placenta.......I am in real need of a good CD31 and CD34 control! We all give our bodies to this - right ;P Caroline Caroline Miller, M.Sc, DIC, AIBMS Senior Research Technologist Director, Histology and Light Microscopy Core Gladstone Institutes 1650 Owens St San Francisco CA 94158 Tel: 415 734 2566 Fax: 415 355 0824 Cell: 415 218 7297 http://www.gladstone.ucsf.edu/gladstone/site/histology/ cmiller@gladstone.ucsf.edu On Mar 28, 2012, at 5:33 PM, Caroline Miller wrote: > > > > > Begin forwarded message: > >> From: "Bartlett, Jeanine (CDC/OID/NCEZID)" >> Date: March 28, 2012 10:41:50 PDT >> To: "O'Donnell, Bill" , Bob Richmond , "histonet@lists.utsouthwestern.edu" >> Subject: RE: [Histonet] "placenta encapsulation" >> >> I thought it was an early April Fool's Day joke...... >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill >> Sent: Wednesday, March 28, 2012 1:41 PM >> To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] "placenta encapsulation" >> >> OK, this is too funny. I swear I got this message concerning my last post. I wonder what triggered it? Are ca**ibals now a protected minori*y? >> >> "This email has violated the R-AC-IAL DIS-CRIMI_NATION. >> and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. >> Message details: >> Server: BCHEXEG >> Sender: (deleted) >> Recipient: rsrichmond@gmail.com;histonet@lists.utsouthwestern.edu; >> Subject: RE: [Histonet] "placenta encapsulation" >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill >> Sent: Wednesday, March 28, 2012 12:33 PM >> To: Bob Richmond; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] "placenta encapsulation" >> >> Now THAT"S NATURAL CHILDBIRTH! >> >> In my 30 years of histology, I have had two such requests. >> (Interestingly, one was about ten years ago - must have been an early >> adopter) >> >> Risk management has a much bigger headache than I do, I just deliver it to their department, they get it to the patient. >> >> Question for histonetters - Is this cannibalism? One of the questions I was never allowed to ask at an interview "What is your feeling about cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, I do not advocate cannibalism, and I am sorry if I offeneded any cannibals (in the vein of redneck lent thread) - Bill >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond >> Sent: Wednesday, March 28, 2012 12:18 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] "placenta encapsulation" >> >> Just when you thought life couldn't get weirder, it does. >> >> I've been hearing scattered tales of women eating their own (actually their babies') placentas for years, but I didn't know about "placenta encapsulation" until my daughter's college classmate Nancy Redd (she's at nancyredd.com) bylined a New York Times story http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent >> a/?scp=1&sq=placenta&st=cse >> >> and then got on ABC news this morning. >> http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 >> >> It seems the pill-'n'-potion culture has come to the rescue, with services (they seem to be local operations, no mail-order, probably how they slip under the regulatory radar) that for $200 or so will dry and grind up your baby's placenta and put it into large gelatin capsules. It has to be your own baby's placenta, by the way. Here's a graphic demonstration of how it's done http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst >> ructions_w_Pictures >> >> And here's a FAQ that answers some, but not all questions a pathology service might have. >> http://birth-wise.org/faq >> >> They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. >> >> Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. >> >> An obvious concern would be bacterial overgrowth. >> >> Eeeeewwww! >> >> Bob Richmond >> Samurai Pathologist >> Knoxville TN >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leswes <@t> shaw.ca Wed Mar 28 21:09:34 2012 From: leswes <@t> shaw.ca (Lesley Weston) Date: Wed Mar 28 21:09:39 2012 Subject: [Histonet] Re: "placenta encapsulation" In-Reply-To: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> References: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> Message-ID: When I had my first baby, someone from the lab where I had been working until just before then arrived to collect the placenta, having asked my permission beforehand. He seemed pretty pleased about it. Lesley. On 03-28-2012, at 6:40 PM, Caroline Miller wrote: > Well....I am 6 months pregnant, and was planning on taking a pot of formalin and a few blades into the delivery suite to get some of my placenta.......I am in real need of a good CD31 and CD34 control! > > We all give our bodies to this - right ;P > > Caroline > > > Caroline Miller, M.Sc, DIC, AIBMS > Senior Research Technologist > Director, Histology and Light Microscopy Core > Gladstone Institutes > 1650 Owens St > San Francisco > CA 94158 > > Tel: 415 734 2566 > Fax: 415 355 0824 > Cell: 415 218 7297 > > http://www.gladstone.ucsf.edu/gladstone/site/histology/ > cmiller@gladstone.ucsf.edu > > > > > > > > > > > > > On Mar 28, 2012, at 5:33 PM, Caroline Miller wrote: > >> >> >> >> >> Begin forwarded message: >> >>> From: "Bartlett, Jeanine (CDC/OID/NCEZID)" >>> Date: March 28, 2012 10:41:50 PDT >>> To: "O'Donnell, Bill" , Bob Richmond , "histonet@lists.utsouthwestern.edu" >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> I thought it was an early April Fool's Day joke...... >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill >>> Sent: Wednesday, March 28, 2012 1:41 PM >>> To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> OK, this is too funny. I swear I got this message concerning my last post. I wonder what triggered it? Are ca**ibals now a protected minori*y? >>> >>> "This email has violated the R-AC-IAL DIS-CRIMI_NATION. >>> and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. >>> Message details: >>> Server: BCHEXEG >>> Sender: (deleted) >>> Recipient: rsrichmond@gmail.com;histonet@lists.utsouthwestern.edu; >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill >>> Sent: Wednesday, March 28, 2012 12:33 PM >>> To: Bob Richmond; histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] "placenta encapsulation" >>> >>> Now THAT"S NATURAL CHILDBIRTH! >>> >>> In my 30 years of histology, I have had two such requests. >>> (Interestingly, one was about ten years ago - must have been an early >>> adopter) >>> >>> Risk management has a much bigger headache than I do, I just deliver it to their department, they get it to the patient. >>> >>> Question for histonetters - Is this cannibalism? One of the questions I was never allowed to ask at an interview "What is your feeling about cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, I do not advocate cannibalism, and I am sorry if I offeneded any cannibals (in the vein of redneck lent thread) - Bill >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond >>> Sent: Wednesday, March 28, 2012 12:18 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] "placenta encapsulation" >>> >>> Just when you thought life couldn't get weirder, it does. >>> >>> I've been hearing scattered tales of women eating their own (actually their babies') placentas for years, but I didn't know about "placenta encapsulation" until my daughter's college classmate Nancy Redd (she's at nancyredd.com) bylined a New York Times story http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent >>> a/?scp=1&sq=placenta&st=cse >>> >>> and then got on ABC news this morning. >>> http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 >>> >>> It seems the pill-'n'-potion culture has come to the rescue, with services (they seem to be local operations, no mail-order, probably how they slip under the regulatory radar) that for $200 or so will dry and grind up your baby's placenta and put it into large gelatin capsules. It has to be your own baby's placenta, by the way. Here's a graphic demonstration of how it's done http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst >>> ructions_w_Pictures >>> >>> And here's a FAQ that answers some, but not all questions a pathology service might have. >>> http://birth-wise.org/faq >>> >>> They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. >>> >>> Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. >>> >>> An obvious concern would be bacterial overgrowth. >>> >>> Eeeeewwww! >>> >>> Bob Richmond >>> Samurai Pathologist >>> Knoxville TN >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Mar 28 21:09:41 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Mar 28 21:09:55 2012 Subject: [Histonet] Re: "placenta encapsulation" In-Reply-To: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> References: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A2516A@xmdb02.nch.kids> What a great idea. Now all I have to do is have a baby and I also can have plenty of control material. Oh bugger, that won't work!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Miller Sent: Thursday, 29 March 2012 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: "placenta encapsulation" Well....I am 6 months pregnant, and was planning on taking a pot of formalin and a few blades into the delivery suite to get some of my placenta.......I am in real need of a good CD31 and CD34 control! We all give our bodies to this - right ;P Caroline Caroline Miller, M.Sc, DIC, AIBMS Senior Research Technologist Director, Histology and Light Microscopy Core Gladstone Institutes 1650 Owens St San Francisco CA 94158 Tel: 415 734 2566 Fax: 415 355 0824 Cell: 415 218 7297 http://www.gladstone.ucsf.edu/gladstone/site/histology/ cmiller@gladstone.ucsf.edu On Mar 28, 2012, at 5:33 PM, Caroline Miller wrote: > > > > > Begin forwarded message: > >> From: "Bartlett, Jeanine (CDC/OID/NCEZID)" >> Date: March 28, 2012 10:41:50 PDT >> To: "O'Donnell, Bill" , Bob Richmond >> , "histonet@lists.utsouthwestern.edu" >> >> Subject: RE: [Histonet] "placenta encapsulation" >> >> I thought it was an early April Fool's Day joke...... >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> O'Donnell, Bill >> Sent: Wednesday, March 28, 2012 1:41 PM >> To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] "placenta encapsulation" >> >> OK, this is too funny. I swear I got this message concerning my last post. I wonder what triggered it? Are ca**ibals now a protected minori*y? >> >> "This email has violated the R-AC-IAL DIS-CRIMI_NATION. >> and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. >> Message details: >> Server: BCHEXEG >> Sender: (deleted) >> Recipient: rsrichmond@gmail.com;histonet@lists.utsouthwestern.edu; >> Subject: RE: [Histonet] "placenta encapsulation" >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> O'Donnell, Bill >> Sent: Wednesday, March 28, 2012 12:33 PM >> To: Bob Richmond; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] "placenta encapsulation" >> >> Now THAT"S NATURAL CHILDBIRTH! >> >> In my 30 years of histology, I have had two such requests. >> (Interestingly, one was about ten years ago - must have been an early >> adopter) >> >> Risk management has a much bigger headache than I do, I just deliver it to their department, they get it to the patient. >> >> Question for histonetters - Is this cannibalism? One of the questions >> I was never allowed to ask at an interview "What is your feeling >> about cannibalism?" Maybe I may allowed to ask it now. - Just having >> some fun, I do not advocate cannibalism, and I am sorry if I >> offeneded any cannibals (in the vein of redneck lent thread) - Bill >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob >> Richmond >> Sent: Wednesday, March 28, 2012 12:18 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] "placenta encapsulation" >> >> Just when you thought life couldn't get weirder, it does. >> >> I've been hearing scattered tales of women eating their own (actually >> their babies') placentas for years, but I didn't know about "placenta >> encapsulation" until my daughter's college classmate Nancy Redd >> (she's at nancyredd.com) bylined a New York Times story >> http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-plac >> ent >> a/?scp=1&sq=placenta&st=cse >> >> and then got on ABC news this morning. >> http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 >> >> It seems the pill-'n'-potion culture has come to the rescue, with >> services (they seem to be local operations, no mail-order, probably >> how they slip under the regulatory radar) that for $200 or so will >> dry and grind up your baby's placenta and put it into large gelatin >> capsules. It has to be your own baby's placenta, by the way. Here's a >> graphic demonstration of how it's done >> http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_I >> nst >> ructions_w_Pictures >> >> And here's a FAQ that answers some, but not all questions a pathology service might have. >> http://birth-wise.org/faq >> >> They want the placenta refrigerated, or possibly frozen. Formaldehyde is prohibited. >> >> Seems to me that this could turn into a major headache for a pathology service, and that some conferring with the necessary people in advance would be a good idea. >> >> An obvious concern would be bacterial overgrowth. >> >> Eeeeewwww! >> >> Bob Richmond >> Samurai Pathologist >> Knoxville TN >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From leswes <@t> shaw.ca Wed Mar 28 21:14:30 2012 From: leswes <@t> shaw.ca (Lesley Weston) Date: Wed Mar 28 21:14:34 2012 Subject: [Histonet] "placenta encapsulation" In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93954D58F2@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF93954D58EE@chimsx08.CHI.catholichealth.net> <4940DF6D1C5FDF48931B6966AAEF93954D58F2@chimsx08.CHI.catholichealth.net> Message-ID: <94D664DA-96A4-42BB-841F-812C4417E943@shaw.ca> Like I said, it just gets funnier and funnier. And I agree with the Samurai Pathologist about the original post in this thread: Eeeewwwwww! Lesley. On 03-28-2012, at 10:40 AM, O'Donnell, Bill wrote: > OK, this is too funny. I swear I got this message concerning my last > post. I wonder what triggered it? Are ca**ibals now a protected > minori*y? > > "This email has violated the R-AC-IAL DIS-CRIMI_NATION. > and Quarantine entire message has been taken on 3/28/2012 1:36:10 PM. > Message details: > Server: BCHEXEG > Sender: (deleted) > Recipient:(deleted) > Subject: RE: [Histonet] "placenta encapsulation" > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > O'Donnell, Bill > Sent: Wednesday, March 28, 2012 12:33 PM > To: Bob Richmond; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] "placenta encapsulation" > > Now THAT"S NATURAL CHILDBIRTH! > > In my 30 years of histology, I have had two such requests. > (Interestingly, one was about ten years ago - must have been an early > adopter) > > Risk management has a much bigger headache than I do, I just deliver it > to their department, they get it to the patient. > > Question for histonetters - Is this cannibalism? One of the questions I > was never allowed to ask at an interview "What is your feeling about > cannibalism?" Maybe I may allowed to ask it now. - Just having some fun, > I do not advocate cannibalism, and I am sorry if I offeneded any > cannibals (in the vein of redneck lent thread) - Bill > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob > Richmond > Sent: Wednesday, March 28, 2012 12:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] "placenta encapsulation" > > Just when you thought life couldn't get weirder, it does. > > I've been hearing scattered tales of women eating their own (actually > their babies') placentas for years, but I didn't know about "placenta > encapsulation" until my daughter's college classmate Nancy Redd (she's > at nancyredd.com) bylined a New York Times story > http://parenting.blogs.nytimes.com/2012/03/25/i-regret-eating-my-placent > a/?scp=1&sq=placenta&st=cse > > and then got on ABC news this morning. > http://abcnews.go.com/GMA/video/eating-placenta-trend-safe-16019081 > > It seems the pill-'n'-potion culture has come to the rescue, with > services (they seem to be local operations, no mail-order, probably how > they slip under the regulatory radar) that for $200 or so will dry and > grind up your baby's placenta and put it into large gelatin capsules. It > has to be your own baby's placenta, by the way. Here's a graphic > demonstration of how it's done > http://www.cafemom.com/journals/read/1577334/Placenta_Encapsulation_Inst > ructions_w_Pictures > > And here's a FAQ that answers some, but not all questions a pathology > service might have. > http://birth-wise.org/faq > > They want the placenta refrigerated, or possibly frozen. Formaldehyde is > prohibited. > > Seems to me that this could turn into a major headache for a pathology > service, and that some conferring with the necessary people in advance > would be a good idea. > > An obvious concern would be bacterial overgrowth. > > Eeeeewwww! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are > not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and delete > the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Thu Mar 29 06:56:09 2012 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Mar 29 06:56:31 2012 Subject: [Histonet] "placenta encapsulation" Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976097B20B3@VHAV09MSGA3.v09.med.va.gov> A few years ago I did see a program from England. The mom took the placenta home in Tupperware put it in the freezer and had a dinner party to which she cooked up the placenta into some sort of spread to be eaten with crackers and they all ate it in "celebration" of the birth of the baby. At the time I grossed several placentas a day and normally like all pathology persons nothing makes me sick, will have to say wanted to become violently ill L. I should be safe on this one, working at a VA hospital no maternity ward! Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From Fawn.Bomar <@t> HalifaxRegional.com Thu Mar 29 07:45:15 2012 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Thu Mar 29 07:45:29 2012 Subject: [Histonet] Annual Competency Checklists Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B92500D9@EXCH-2K10.hrhs.com> Hello Everyone! Does anybody have an annual competency checklist for their employees that they are willing to share? Thank you Fawn From SDattili <@t> stormontvail.org Thu Mar 29 08:31:27 2012 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Thu Mar 29 08:31:43 2012 Subject: [Histonet] Evaluation of interobserver variability among pathologists Message-ID: Hi all, Could you please share how your laboratory evaluates interobserver variability among the pathologists per CAP checklist item ANP.22970 (Annual Result Comparison)? I would like to evaluate if my procedure is adequate of if there is more that I should be doing. Does the checklist item imply that we compare the results by individual pathologist to the published benchmarks? Or does it mean that we compare the scoring of each pathologist on the same set of cases? Or something else? Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas P.S. Hi Scott! Thanks again for the great inspection yesterday. NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From mweirauch <@t> crittenton.com Thu Mar 29 09:14:39 2012 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Thu Mar 29 09:17:00 2012 Subject: [Histonet] Evaluation of interobserver variability among pathologists In-Reply-To: References: Message-ID: We subscribe to the PM Immunohistochemistry CAP Surveys. (Each time we receive a new survey, they are given to a different pathologist so they each have a turn at submitting results to the CAP. Can't say that they are fighting over them-haha) When you go on-line to the CAP site to input results, you can print extra blank copies of the result form. I will print several extra copies and hold them. When the survey due date is past and the survey can be shared with other people, the slides and extra blank forms are passed around to all the rest of the pathologists for their input. I collect all the results and put them in a spread sheet that lines up each pathologists' answers side by side along with the CAP expected result for comparison. This is how we evaluate interobserver variability among our pathologists, nothing more. We also compare our lab results bi-annually with the benchmarks, but this is not broken down by individual pathologist. Maray -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, March 29, 2012 8:31 AM To: Histonet Listserv (E-mail) Subject: [Histonet] Evaluation of interobserver variability among pathologists Hi all, Could you please share how your laboratory evaluates interobserver variability among the pathologists per CAP checklist item ANP.22970 (Annual Result Comparison)? I would like to evaluate if my procedure is adequate of if there is more that I should be doing. Does the checklist item imply that we compare the results by individual pathologist to the published benchmarks? Or does it mean that we compare the scoring of each pathologist on the same set of cases? Or something else? Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas P.S. Hi Scott! Thanks again for the great inspection yesterday. NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Mar 29 09:24:01 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Mar 29 09:24:33 2012 Subject: [Histonet] Evaluation of interobserver variability among pathologists In-Reply-To: References: , Message-ID: This is exactly what we do as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch [mweirauch@crittenton.com] Sent: Thursday, March 29, 2012 10:14 AM To: Histonet Listserv (E-mail) Subject: RE: [Histonet] Evaluation of interobserver variability among pathologists We subscribe to the PM Immunohistochemistry CAP Surveys. (Each time we receive a new survey, they are given to a different pathologist so they each have a turn at submitting results to the CAP. Can't say that they are fighting over them-haha) When you go on-line to the CAP site to input results, you can print extra blank copies of the result form. I will print several extra copies and hold them. When the survey due date is past and the survey can be shared with other people, the slides and extra blank forms are passed around to all the rest of the pathologists for their input. I collect all the results and put them in a spread sheet that lines up each pathologists' answers side by side along with the CAP expected result for comparison. This is how we evaluate interobserver variability among our pathologists, nothing more. We also compare our lab results bi-annually with the benchmarks, but this is not broken down by individual pathologist. Maray -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, March 29, 2012 8:31 AM To: Histonet Listserv (E-mail) Subject: [Histonet] Evaluation of interobserver variability among pathologists Hi all, Could you please share how your laboratory evaluates interobserver variability among the pathologists per CAP checklist item ANP.22970 (Annual Result Comparison)? I would like to evaluate if my procedure is adequate of if there is more that I should be doing. Does the checklist item imply that we compare the results by individual pathologist to the published benchmarks? Or does it mean that we compare the scoring of each pathologist on the same set of cases? Or something else? Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas P.S. Hi Scott! Thanks again for the great inspection yesterday. NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.Freeman <@t> fda.hhs.gov Thu Mar 29 09:32:59 2012 From: Lisa.Freeman <@t> fda.hhs.gov (Freeman, Lisa*) Date: Thu Mar 29 09:33:09 2012 Subject: [Histonet] Microwave processor Message-ID: <67FEC78882E60A48B275A63143BAA0F519A4566B6B@FDSWV2150.fda.gov> I will soon be in the market for our first microwave tissue processor. Any information / favorite, would be greatly appreciated. Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov From jqb7 <@t> cdc.gov Thu Mar 29 09:36:50 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Mar 29 09:36:58 2012 Subject: [Histonet] RE: Microwave processor In-Reply-To: <67FEC78882E60A48B275A63143BAA0F519A4566B6B@FDSWV2150.fda.gov> References: <67FEC78882E60A48B275A63143BAA0F519A4566B6B@FDSWV2150.fda.gov> Message-ID: I love our Sakura Xpress. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Lisa D. (FDA/NCTR/OCS) (CTR) Sent: Thursday, March 29, 2012 10:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microwave processor I will soon be in the market for our first microwave tissue processor. Any information / favorite, would be greatly appreciated. Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 29 09:53:52 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 29 09:53:59 2012 Subject: [Histonet] Microwave processor In-Reply-To: <67FEC78882E60A48B275A63143BAA0F519A4566B6B@FDSWV2150.fda.gov> Message-ID: <1333032832.47445.YahooMailClassic@web162103.mail.bf1.yahoo.com> Check with your Milestone representative. Ren? J. --- On Thu, 3/29/12, Freeman, Lisa* wrote: From: Freeman, Lisa* Subject: [Histonet] Microwave processor To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, March 29, 2012, 10:32 AM I will soon be in the market for our first microwave tissue processor. Any information / favorite, would be greatly appreciated. Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Mar 29 10:03:00 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Mar 29 10:03:14 2012 Subject: [Histonet] "placenta encapsulation" In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF3976097B20B3@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF3976097B20B3@VHAV09MSGA3.v09.med.va.gov> Message-ID: Back in 1974 in Louisville, KY I agreed to give the placenta from my firstborn over for some research project. I didn't ask at the time what the heck they were researching - I was in labor and I just wanted it OUT. I hope they didn't eat it! Or make shampoo out of it. Eeeewwwwww! Andi G. On Mar 29, 2012, at 4:56 AM, White, Lisa M. wrote: > A few years ago I did see a program from England. The mom took the > placenta home in Tupperware put it in the freezer and had a dinner party > to which she cooked up the placenta into some sort of spread to be eaten > with crackers and they all ate it in "celebration" of the birth of the > baby. At the time I grossed several placentas a day and normally like > all pathology persons nothing makes me sick, will have to say wanted to > become violently ill L. I should be safe on this one, working at a VA > hospital no maternity ward! > > > > Lisa White, HT(ASCP) > > Supervisory HT > > James H. Quillen VAMC > > PO Box 4000 > > Corner of Veterans Way and Lamont > > PLMS 113 > > Mountain Home, TN 37684 > > 423-979-3567 > > 423-979-3401 fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brannon <@t> alliedsearchpartners.com Thu Mar 29 10:44:48 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Mar 29 10:45:08 2012 Subject: [Histonet] Histology Technician (Clinical Lab Technician-Histology) Message-ID: Allied Search Partners is working with a laboratory in Southern CA looking for a Histology professional. To Apply Please email or fax resume to Brannon@alliedsearchpartners.com or fax to 888 388 7572. No other information is given about location or the organization at this time. Please send resume for review by our recruiters and all qualified candidates will be given a phone call to discuss further. Thank you! Location: Oceanside, CA area Schedule: Full Time/Permanent Title: Histology Technician (Clinical Lab Technician-Histology) Summary The Clinical Lab Technician I will be responsible to assist in the pre-analytical, and post-analytical processes associated with specimen processing and test performance. The Clinical Lab Technician I will also prepare and stain sections from formalin-fixed, paraffin-embedded tissue specimens for routine and special procedures. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rjbuesa <@t> yahoo.com Thu Mar 29 10:51:19 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 29 10:51:23 2012 Subject: [Histonet] RE: Microwave processor In-Reply-To: Message-ID: <1333036279.98287.YahooMailClassic@web162102.mail.bf1.yahoo.com> The pricey Sakura Xpress is a "mixed-technology" tissue processor with the first?2 (or 1 in the smaller version) chambers with microwave technology and the other 2 (or 1) with "conventional" (non-microwave) technology. Ren? J. --- On Thu, 3/29/12, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] RE: Microwave processor To: "Freeman, Lisa D. (FDA/NCTR/OCS) (CTR)" , "'histonet@lists.utsouthwestern.edu'" Date: Thursday, March 29, 2012, 10:36 AM I love our Sakura Xpress. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Lisa D. (FDA/NCTR/OCS) (CTR) Sent: Thursday, March 29, 2012 10:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microwave processor I will soon be in the market for our first microwave tissue processor. Any information / favorite, would be greatly appreciated. Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estellamireles <@t> gmail.com Thu Mar 29 11:19:34 2012 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Thu Mar 29 11:19:38 2012 Subject: [Histonet] Need the name of a piece of equipment Message-ID: Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella From wbenton <@t> cua.md Thu Mar 29 11:26:16 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Mar 29 11:26:37 2012 Subject: [Histonet] Need the name of a piece of equipment - para trimmer Message-ID: <0B8979A204680A42B93A52B486088CD92B6A285F9A@CUAEXH1.GCU-MD.local> http://www.thermoscientific.com/ecomm/servlet/productsdetail_11152_L10744_80555_12933395_-1 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles [estellamireles@gmail.com] Sent: Thursday, March 29, 2012 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From brannon <@t> alliedsearchpartners.com Thu Mar 29 11:26:28 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Mar 29 11:26:43 2012 Subject: [Histonet] HT/HTL Opening in Southern CA Message-ID: Allied Search Partners is working with a laboratory in Southern CA looking for a qualified and licensed Histotechnologist or Histotechnician. To Apply Please email or fax resume to Brannon@alliedsearchpartners.com or fax to 888 388 7572. No other information is given about location or the organization at this time. Please send resume for review by our recruiters and all qualified candidates will be given a phone call to discuss further. Thank you! Location: Oceanside, CA area Schedule: Full Time/Permanent Title: Histotechnician (HT) or Histotechnologist (HTL) depending on education and certification. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From lblazek <@t> digestivespecialists.com Thu Mar 29 11:31:47 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 29 11:31:57 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E3913819D437D@IBMB7Exchange.digestivespecialists.com> http://www.mercedesmedical.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, March 29, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Thu Mar 29 11:35:22 2012 From: jstaruk <@t> masshistology.com (jstaruk) Date: Thu Mar 29 11:35:29 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <025a01cd0dc9$f0cdf730$d269e590$@masshistology.com> http://www.walmart.com/ip/Rival-20-Electric-Griddle/16451315 $18.96 _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, March 29, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4902 - Release Date: 03/29/12 From lblazek <@t> digestivespecialists.com Thu Mar 29 11:43:24 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 29 11:43:33 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: <025a01cd0dc9$f0cdf730$d269e590$@masshistology.com> References: <025a01cd0dc9$f0cdf730$d269e590$@masshistology.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3913819D437E@IBMB7Exchange.digestivespecialists.com> LOL Good one! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Thursday, March 29, 2012 12:35 PM To: 'Stella Mireles'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Need the name of a piece of equipment http://www.walmart.com/ip/Rival-20-Electric-Griddle/16451315 $18.96 _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, March 29, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2114/4902 - Release Date: 03/29/12 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Mar 29 11:47:35 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Mar 29 11:47:47 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <090FA56107A969459F3941DDD5585C3A06919845@PHSX10MB10.partners.org> I can't compete with the WalMart griddle(!), but EMS (Electron Microscopy Sciences) sells the same para-trimmer for considerably less than Fisher ($827) or Mercedes Medical ($724.50). EMS's price $350.00 (Cat. # 62850-10, 115VAC). We have one and it works just fine. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, March 29, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From jaylundgren <@t> gmail.com Thu Mar 29 11:49:21 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Mar 29 11:49:29 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: <025a01cd0dc9$f0cdf730$d269e590$@masshistology.com> References: <025a01cd0dc9$f0cdf730$d269e590$@masshistology.com> Message-ID: On Thu, Mar 29, 2012 at 11:35 AM, jstaruk wrote: > http://www.walmart.com/ip/Rival-20-Electric-Griddle/16451315 > > $18.96 > > > LOL > > > > > > > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1913 / Virus Database: 2114/4902 - Release Date: 03/29/12 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Thu Mar 29 11:55:43 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Mar 29 11:55:46 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: Hey, when you find out the name, send it to the histonet list. I'm curious as to what it's called. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Thu, Mar 29, 2012 at 12:19 PM, Stella Mireles wrote: > Good day to all, > I need the name of the equipment that melts the wax off the edges of a > paraffin block. > Where can I purchase it. > > Thank You > Stella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Glark <@t> NSHS.edu Thu Mar 29 12:21:50 2012 From: Glark <@t> NSHS.edu (Lark, Gayle P) Date: Thu Mar 29 12:24:05 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <5D695559785693428F529FB5D5FC5A1143B0BE2823@SYKECHXVS04.nslijhs.net> That is called a wax trimmer.Wehave an old one made by Premerie. Gayle Gayle Lark MS Histology Supervisor Southside Hospital 301 E. Main Street Bay Shore, NY 11706 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles [estellamireles@gmail.com] Sent: Thursday, March 29, 2012 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. From mfisher <@t> ecrmc.org Thu Mar 29 12:34:17 2012 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu Mar 29 12:34:29 2012 Subject: [Histonet] Placenta Helper Message-ID: Placenta eaters have been around for a long time. In fact, many years ago on the original Saturday Night Live, Jane Curtin and Laraine Newman did an absolutely hilarious skit on Placenta Helper (Hamburger Helper). You can probably find it online. It's also been known that fresh placentas make great rose bush fertilizer and catfish bait! Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From DKBoyd <@t> chs.net Thu Mar 29 12:41:39 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Mar 29 12:41:50 2012 Subject: [Histonet] IHC charges Message-ID: <7EAFE982E328304DA6CE2B677BB762460BACCD33@TN001WEXMBX12.US.chs.net> Can I get the synoptic version of the IHC charge, changes? IE; each antibody or each block? Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From DKBoyd <@t> chs.net Thu Mar 29 12:44:24 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Mar 29 12:44:34 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB762460BACCDBB@TN001WEXMBX12.US.chs.net> Fisher/Shandon sells it. It is called the ParaTrimmer. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Stella Mireles [estellamireles@gmail.com] Sent: Thursday, March 29, 2012 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From MSHERWOOD <@t> PARTNERS.ORG Thu Mar 29 13:05:15 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Mar 29 13:05:23 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <090FA56107A969459F3941DDD5585C3A069198B0@PHSX10MB10.partners.org> The one from EMS is called Wax Trimmer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 29, 2012 12:56 PM To: Stella Mireles; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need the name of a piece of equipment Hey, when you find out the name, send it to the histonet list. I'm curious as to what it's called. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that's beautiful. --Ron Swanson On Thu, Mar 29, 2012 at 12:19 PM, Stella Mireles wrote: > Good day to all, > I need the name of the equipment that melts the wax off the edges of a > paraffin block. > Where can I purchase it. > > Thank You > Stella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From TGoins <@t> mt.gov Thu Mar 29 13:25:17 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Mar 29 13:25:28 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: Found a wax trimmer from Ted Pella for less than $300 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, March 29, 2012 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Mar 29 14:00:46 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Mar 29 14:00:53 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <090FA56107A969459F3941DDD5585C3A0691990F@PHSX10MB10.partners.org> Thanks, Tresa. I stand corrected. I did order ours from Ted Pella @ a year ago for $289. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Thursday, March 29, 2012 2:25 PM To: 'Stella Mireles'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need the name of a piece of equipment Found a wax trimmer from Ted Pella for less than $300 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, March 29, 2012 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Jessica <@t> nsh.org Thu Mar 29 14:43:56 2012 From: Jessica <@t> nsh.org (Jessica Smith) Date: Thu Mar 29 15:09:01 2012 Subject: [Histonet] NSH 5th Annual Summer Symposium Message-ID: <2B6E973D7D12964F8D8A3598557C5530052D3B@NSH-SRVR01.nsh.local> The National Society for Histotechnology is bringing the 5th Annual Summer Symposium back to Las Vegas, NV! General sessions and workshops featuring expert speakers will provide you with the tools, advice and guidance you seek in your professional career. The Summer Symposium is one of the best values for your limited training dollars in histology education offering 12 continuing education credits and an Exhibit Fair for one low price! Registration Fees: Member: $229 Non-Member: $249 Student Member: $169 HT Readiness Course Only (does not include exhibit fair): $99 This fantastic event will be held at the Planet Hollywood Resort & Casino right in the center of all the Las Vegas Entertainment! For more information regarding the agenda, travel information, and to register visit: http://s3.goeshow.com/nsh/SS2012/ereg419568.cfm?clear Or register by mail/fax: http://www.nsh.org/sites/default/files/Reg.%20Form.pdf Any questions or concerns please contact Jessica Smith at 443.535.4062 or jessica@nsh.org Jessica Smith Meeting Coordinator National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From amosbrooks <@t> gmail.com Thu Mar 29 15:38:50 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Mar 29 15:38:54 2012 Subject: [Histonet] Re: "placenta encapsulation" Message-ID: Hi, I would recommend getting someone else to do it for you. Unless you are *really* resilient, I would expect you would have other recovery related things to think about. With the OB/GYN's permission (and curiosity) I took my wife's placenta twice. Once for each kid. The local college was grateful to have it since it is a great tissue to learn on, and I also had an abundance of control tissue. The shock on my mother-in-law's face was palpable. She still thinks I'm nuts. Congratulations & Good Luck, Amos On Wed, Mar 28, 2012 at 10:10 PM, wrote: > Message: 14 > Date: Wed, 28 Mar 2012 18:40:39 -0700 > From: Caroline Miller > Subject: [Histonet] Re: "placenta encapsulation" > To: histonet@lists.utsouthwestern.edu > Message-ID: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Well....I am 6 months pregnant, and was planning on taking a pot of > formalin and a few blades into the delivery suite to get some of my > placenta.......I am in real need of a good CD31 and CD34 control! > > We all give our bodies to this - right ;P > > Caroline > > From tony.henwood <@t> health.nsw.gov.au Thu Mar 29 17:43:16 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 29 17:43:30 2012 Subject: [Histonet] Need the name of a piece of equipment In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A25493@xmdb02.nch.kids> Thermo's Para-Trimer is probably what you are after. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, 30 March 2012 3:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need the name of a piece of equipment Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From cmhernandez78 <@t> me.com Thu Mar 29 18:21:31 2012 From: cmhernandez78 <@t> me.com (Carlos Hernandez) Date: Thu Mar 29 18:21:43 2012 Subject: [Histonet] Microwave processor In-Reply-To: <67FEC78882E60A48B275A63143BAA0F519A4566B6B@FDSWV2150.fda.gov> References: <67FEC78882E60A48B275A63143BAA0F519A4566B6B@FDSWV2150.fda.gov> Message-ID: <0841DF3F-B48B-4C34-9443-EE3D7101F8E9@me.com> I absolutely love the Milestone Pathos Delta, which is fully automated that can be used formalin and xylene free without having to buy proprietary reagents. They also have a variety of options based on your type of lab and workload. Feel free to contact me if you have more specific questions. Carlos On Mar 29, 2012, at 8:32 AM, "Freeman, Lisa*" wrote: > I will soon be in the market for our first microwave tissue processor. Any information / favorite, would be greatly appreciated. > > Lisa Freeman, HT > Histology Supervisor > Toxicologic Pathology Associates > National Center for Toxicological Research > 3900 NCTR RD > Jefferson AR 72079 > Phone: 870-543-7234 > E-mail: Lisa.Freeman@fda.hhs.gov > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwahlsten <@t> yahoo.com Thu Mar 29 20:30:50 2012 From: mwahlsten <@t> yahoo.com (Michelle Wahlsten) Date: Thu Mar 29 20:31:54 2012 Subject: [Histonet] Leica Peloris II processor validation Message-ID: <1333071050.94140.YahooMailNeo@web111202.mail.gq1.yahoo.com> Hi everyone, I'm currently in process of validating the Peloris II for our lab, my first instrument validation so I'm a bit lost as to what specifically I need to do and document.? Other than running specimens through the specific protocols to verify the quality of the specimen with a pathologist's review and/or sign-off, I feel that there is more that I need to do but I'm not sure what that is.? Can anyone share with me their experience of the validation process and/or suggestions as to what else I need or what I'm missing?? In addition to the instrument validation itself, we also need to validate IHC use on the Peloris, per one of our pathologist's.? How many antibodies, controls or panels should we be doing to satisfy the 'validation' process for IHC??? Any information will be helpful, thank you so much!! Michelle Wahlsten, HTL University of Minnesota Medical Center, Fairview Minneapolis, MN From d-emge <@t> northwestern.edu Thu Mar 29 23:35:42 2012 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Thu Mar 29 23:35:46 2012 Subject: [Histonet] Histology Position 12hrs to 15hrs per week, downtown Chicago, IL Message-ID: <54C3042A8564BA4AA2D355DD1D4C949C1071BA56@chcspmbx2.ads.northwestern.edu> Histology position available 12hrs to 15hrs per week with flexible scheduling at one of the top research core laboratories at Northwestern University, Feinberg School of Medicine, in the heart of downtown Chicago, IL. This is not the typical clinical grind, it is a laboratory that provides histology service university wide to researchers from the Evanston and Chicago campus.' Send resume to mhpl@northwestern.edu From sbreeden <@t> nmda.nmsu.edu Fri Mar 30 08:16:14 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Mar 30 08:16:34 2012 Subject: [Histonet] Farewell Message-ID: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From lblazek <@t> digestivespecialists.com Fri Mar 30 08:21:23 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Mar 30 08:21:33 2012 Subject: [Histonet] RE: Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E3913819D4380@IBMB7Exchange.digestivespecialists.com> Ah... We'll miss you Sally! You have been a great source of wisdom, humor, my off line bantering partner and my conference cohort. But I know where you are! Remember to take a picture of the sunrise on Monday morning. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, March 30, 2012 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Mar 30 08:24:20 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri Mar 30 08:24:32 2012 Subject: [Histonet] RE: Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <090FA56107A969459F3941DDD5585C3A0691A22E@PHSX10MB10.partners.org> Best wishes, Sally. I hope you do fun things in retirement. I know you will "wake up and smell the coffee", just not at that ungodly hour of 3:45am! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine-EDR 214 50 Blossom Street Boston, MA 02114 617-724-4839 (voice) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, March 30, 2012 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From jqb7 <@t> cdc.gov Fri Mar 30 08:25:25 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Mar 30 08:25:54 2012 Subject: [Histonet] RE: Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Congratulations and good for you! No one should wait until it is too late to enjoy life. Hopefully I won't be far behind! You will be missed. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, March 30, 2012 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 30 08:34:16 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 30 08:34:24 2012 Subject: [Histonet] Leica Peloris II processor validation In-Reply-To: <1333071050.94140.YahooMailNeo@web111202.mail.gq1.yahoo.com> Message-ID: <1333114456.55595.YahooMailClassic@web162102.mail.bf1.yahoo.com> CAP has a "suggestion" for a validation protocol BUT the most important thing is the acceptance of your processed tissues by the pathologist. S/he will be the ultimate responsible in the validation process. Ideally, you should run parallel samples with the instrument you are using now and the Peloris. The number varies. You will also have to do IHC in some of the samples run with the Peloris. Select those IHC of greater importance and most frequent use. Ask your pathologist which s/he wants. In all these validations do not try to do it by yourself, always ask the pathologists what s/he wants to do. At first glance validation seems to be a daunting task but it is not if you follow your pathologists' indications. Document the whole process and make sure to have the pathologist's signature on the evaluation of the slides and procedures. Archive those blocks and slides for future reference. That is how I did all my validations even when I was developing new processing protocols. Ren? J. --- On Thu, 3/29/12, Michelle Wahlsten wrote: From: Michelle Wahlsten Subject: [Histonet] Leica Peloris II processor validation To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, March 29, 2012, 9:30 PM Hi everyone, I'm currently in process of validating the Peloris II for our lab, my first instrument validation so I'm a bit lost as to what specifically I need to do and document.? Other than running specimens through the specific protocols to verify the quality of the specimen with a pathologist's review and/or sign-off, I feel that there is more that I need to do but I'm not sure what that is.? Can anyone share with me their experience of the validation process and/or suggestions as to what else I need or what I'm missing?? In addition to the instrument validation itself, we also need to validate IHC use on the Peloris, per one of our pathologist's.? How many antibodies, controls or panels should we be doing to satisfy the 'validation' process for IHC??? Any information will be helpful, thank you so much!! Michelle Wahlsten, HTL University of Minnesota Medical Center, Fairview Minneapolis, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 30 08:41:37 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 30 08:41:45 2012 Subject: [Histonet] Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1333114897.962.YahooMailClassic@web162106.mail.bf1.yahoo.com> Sarah: It has been always a pleasure communicating with you as we have done so many times in the past.??You are a real specialist in the difficult field of veterinary histology and you should remember that when in retirement. Try to pass on your knowledge. Being retired does not mean to go into "mental hibernation". Participate in HistoNet even more than before. Now you are the owner of your time. My sincere congratulations and enjoy your retirement as I have been doing for the last 10 years. Ren? J. --- On Fri, 3/30/12, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Farewell To: histonet@lists.utsouthwestern.edu Date: Friday, March 30, 2012, 9:16 AM Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work.? Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday!? Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience.? I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m.? Thank you all for your advice, guidance, humor, relative insanity and wisdom.? Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Mar 30 09:18:35 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Mar 30 09:18:42 2012 Subject: [Histonet] Farewell In-Reply-To: <1333114897.962.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> <1333114897.962.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: Sally, I will miss our on- and off- topic conversations! It's been invaluable bantering back and forth with you regarding IHC in veterinary medicine, with all of its 'adventures in staining'. You must promise to enjoy every second of your retirement - do it for 'the team'. And keep posting your humorous quips on Histonet. Jan Shivers On Fri, Mar 30, 2012 at 8:41 AM, Rene J Buesa wrote: > Sarah: > It has been always a pleasure communicating with you as we have done so > many times in the past. You are a real specialist in the difficult field > of veterinary histology and you should remember that when in retirement. > Try to pass on your knowledge. Being retired does not mean to go into > "mental hibernation". > Participate in HistoNet even more than before. Now you are the owner of > your time. > My sincere congratulations and enjoy your retirement as I have been doing > for the last 10 years. > Ren? J. > > --- On Fri, 3/30/12, Breeden, Sara wrote: > > > From: Breeden, Sara > Subject: [Histonet] Farewell > To: histonet@lists.utsouthwestern.edu > Date: Friday, March 30, 2012, 9:16 AM > > > Hard as it is to believe - and I probably won't until I've been away for > a week or more - this is my LAST day to work. Retirement? Who knew it > would come around so fast? I remember having only fifteen years left to > work and that was just yesterday! Histology has been 'bery, 'bery good > to me and although I fell into it by accident, it has been a > fascinating, involving, liberating experience. I'll be lurking on > Histonet but under the alias of nmhisto and I'll probably do some p.r.n. > work but I'm going home and throwing away my alarm clock and resetting > the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for > your advice, guidance, humor, relative insanity and wisdom. Enjoy what > you do and try to recruit at least one person into this field before > it's your time to pick out the color of the tennis balls on your walker. > > > > Hail and Farewell and best of everything to every one of you. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mturner <@t> CSILaboratories.com Fri Mar 30 09:24:00 2012 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Mar 30 09:24:09 2012 Subject: [Histonet] Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <467983BDAC5880438E2202F6B9BF79A84C9F4C@exchange-be-01.CSI-LABS.local> Congratulations on a retirement well earned. I have always valued your insight into the problems posed on the histonet. Keep in touch and continue investing in the next generation of histotechs, both through the histonet and in any speaking engagements you can line up. Try to get some rest, but my guess is that you will continue to wake up early for a few more weeks. After all, one knows that someone is a histotech when waking up at 4:30 AM is considered "sleeping in!" :-)) Best wishes on your retirement! Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: Friday, March 30, 2012 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Mar 30 09:43:29 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Mar 30 09:43:42 2012 Subject: [Histonet] Say Just for a while In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE24B32F2C4DE@MERCERMAIL.MercerU.local> Well I bet you will be back in a week. This histology family is hard to stay away from. :) I am coming up on 50 years in July, but not ready to leave it. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, March 30, 2012 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Fri Mar 30 09:48:45 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Fri Mar 30 09:51:15 2012 Subject: [Histonet] RE: Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <2346AA5F03D2D04CAC9DB1C25B3F10C90AC69E30E8@VA3DIAXVS451.RED001.local> I'm just trying to get a replacement hired so I can spend the next 2 yrs teaching him all I know about histology! Well some of what I know. Hiring process here is a !@#$%. Gook luck Sally! Happy, happy retirement!!!!!!!!!!!!! Andi ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Friday, March 30, 2012 6:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From t.magee <@t> gipath.com Fri Mar 30 10:35:21 2012 From: t.magee <@t> gipath.com (Tiffeny Magee) Date: Fri Mar 30 10:35:35 2012 Subject: [Histonet] Skin Biopsy Neuropathy PGP 9.5 Message-ID: Happy Friday fellow Histo's does anyone have hands on experience with testing intraepidermal nerve fibers. This test is assessing intrapedimeral nerve fibers density using skin biopsy and immunostaining (PGP 9.5 -13C4). This identification allows the pathologist to count the small nerve fibers. I am unable to locate a protocol with all the specifics to make this work. I have been tweaking it on paraffin embedded tissue but only have seen a small amount of nerves. Anyone have a successful method to make this work? Thank You, Tiffeny Magee From contact <@t> excaliburpathology.com Fri Mar 30 10:55:49 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Mar 30 10:55:54 2012 Subject: [Histonet] Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1333122949.69032.YahooMailNeo@web5715.biz.mail.ne1.yahoo.com> Congrats!!! & Enjoy! See you under your new handle. :) ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Breeden, Sara" To: histonet@lists.utsouthwestern.edu Sent: Friday, March 30, 2012 8:16 AM Subject: [Histonet] Farewell Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work.? Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday!? Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience.? I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m.? Thank you all for your advice, guidance, humor, relative insanity and wisdom.? Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Mar 30 10:58:26 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Mar 30 10:58:32 2012 Subject: [Histonet] Reference lab doing West Nile Virus and HHV6 IHC on human FFPE specimens Message-ID: Yup, that's what I'm looking for Histonetters. Any info. is appreciated. Linda From shive003 <@t> umn.edu Fri Mar 30 11:03:18 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Mar 30 11:03:25 2012 Subject: [Histonet] Reference lab doing West Nile Virus and HHV6 IHC on human FFPE specimens In-Reply-To: References: Message-ID: Aww, too bad. We do WNV testing on FFPE, but not on human tissue. BioReliance sells a good antibody (clone 7H2), in case anyone else out there wants to know a vendor for the antibody. Enzyme digestion works OK for antigen retrieval, but HIER works much better at exposing epitopes. Jan Shivers UMN Vet Diag Lab St. Paul, MN On Fri, Mar 30, 2012 at 10:58 AM, Sebree Linda A wrote: > Yup, that's what I'm looking for Histonetters. > > Any info. is appreciated. > > Linda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Fri Mar 30 11:05:01 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Mar 30 11:05:10 2012 Subject: [Histonet] Reference lab doing West Nile Virus and HHV6 IHC on human FFPE specimens In-Reply-To: References: Message-ID: Clarification to my previous post - we can't *accept* human tissue here. I'm sure the antibody will work on any species, however. The antibody is specific to the virus itself, not the tissue species it's residing in. Jan Shivers On Fri, Mar 30, 2012 at 11:03 AM, Jan Shivers wrote: > Aww, too bad. We do WNV testing on FFPE, but not on human tissue. > BioReliance sells a good antibody (clone 7H2), in case anyone else out > there wants to know a vendor for the antibody. Enzyme digestion works OK > for antigen retrieval, but HIER works much better at exposing epitopes. > > Jan Shivers > UMN Vet Diag Lab > St. Paul, MN > > > On Fri, Mar 30, 2012 at 10:58 AM, Sebree Linda A wrote: > >> Yup, that's what I'm looking for Histonetters. >> >> Any info. is appreciated. >> >> Linda >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From mamawooo <@t> hotmail.com Fri Mar 30 12:53:28 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Mar 30 12:53:34 2012 Subject: [Histonet] Farewell In-Reply-To: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Enjoy retirement Sara, I'm loving it and have been for almost a year now. Still love and am very interested in Histology. I think it stays in the blood forever. I'm doing a little consulting but mostly enjoying my grandson and being home. Never a boring minute, I'm as busy as I ever was, Congratulations!Jan MahoneyOmaha > Date: Fri, 30 Mar 2012 07:16:14 -0600 > From: sbreeden@nmda.nmsu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Farewell > > Hard as it is to believe - and I probably won't until I've been away for > a week or more - this is my LAST day to work. Retirement? Who knew it > would come around so fast? I remember having only fifteen years left to > work and that was just yesterday! Histology has been 'bery, 'bery good > to me and although I fell into it by accident, it has been a > fascinating, involving, liberating experience. I'll be lurking on > Histonet but under the alias of nmhisto and I'll probably do some p.r.n. > work but I'm going home and throwing away my alarm clock and resetting > the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for > your advice, guidance, humor, relative insanity and wisdom. Enjoy what > you do and try to recruit at least one person into this field before > it's your time to pick out the color of the tennis balls on your walker. > > > > Hail and Farewell and best of everything to every one of you. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Fri Mar 30 12:55:59 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Mar 30 12:56:04 2012 Subject: [Histonet] Farewell In-Reply-To: References: <02C099024072804EA34F5906BAC30A413F2043@nmdamailsvr.nmda.ad.nmsu.edu>, Message-ID: I meant Sally, sorry. > From: mamawooo@hotmail.com > To: sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu > Date: Fri, 30 Mar 2012 07:53:28 -1000 > Subject: RE: [Histonet] Farewell > CC: > > > Enjoy retirement Sara, I'm loving it and have been for almost a year now. Still love and am very interested in Histology. I think it stays in the blood forever. I'm doing a little consulting but mostly enjoying my grandson and being home. Never a boring minute, I'm as busy as I ever was, > Congratulations!Jan MahoneyOmaha > > > Date: Fri, 30 Mar 2012 07:16:14 -0600 > > From: sbreeden@nmda.nmsu.edu > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Farewell > > > > Hard as it is to believe - and I probably won't until I've been away for > > a week or more - this is my LAST day to work. Retirement? Who knew it > > would come around so fast? I remember having only fifteen years left to > > work and that was just yesterday! Histology has been 'bery, 'bery good > > to me and although I fell into it by accident, it has been a > > fascinating, involving, liberating experience. I'll be lurking on > > Histonet but under the alias of nmhisto and I'll probably do some p.r.n. > > work but I'm going home and throwing away my alarm clock and resetting > > the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for > > your advice, guidance, humor, relative insanity and wisdom. Enjoy what > > you do and try to recruit at least one person into this field before > > it's your time to pick out the color of the tennis balls on your walker. > > > > > > > > Hail and Farewell and best of everything to every one of you. > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laurie <@t> blufrogpath.com Fri Mar 30 13:53:21 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Fri Mar 30 13:53:26 2012 Subject: [Histonet] Per Diem HT Position, Los Angeles Message-ID: <20120330115321.295dc6182df7e5cbb4f32bc101c30dcc.5eb4f75707.wbe@email15.secureserver.net> Hi Histonetters, I am working fo (very close to Cedars Si ASCP certified histotech to wo person will help cover the lab when or when the workload is high - so the ho consistent. We need someone that is experienced in a histology (embedding, cutting, special stains) and is able to well on their own with little guidance. Good troubleshooting sk are also required. The hours are somewhat flexible, and the pay will be based on experience but is very competitive. Please forward your resume to me if you are interested, or feel to email me with any questions you may have. Thanks! Laurie Colbert HT (ASCP) BluFrog Los Angeles, CA From ander093 <@t> umn.edu Fri Mar 30 16:50:21 2012 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Fri Mar 30 16:50:30 2012 Subject: [Histonet] Farewell In-Reply-To: <1333114897.962.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <1333114897.962.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: <4F762A9D.4040404@umn.edu> Wishing you all the best in your retirement, Sally !! Do try to keep in touch though~~your advice and expertise will be missed !!! Enjoy! LuAnn On 3/30/2012 8:41 AM, Rene J Buesa wrote: > Sarah: > It has been always a pleasure communicating with you as we have done so many times in the past. You are a real specialist in the difficult field of veterinary histology and you should remember that when in retirement. > Try to pass on your knowledge. Being retired does not mean to go into "mental hibernation". > Participate in HistoNet even more than before. Now you are the owner of your time. > My sincere congratulations and enjoy your retirement as I have been doing for the last 10 years. > Ren? J. > > --- On Fri, 3/30/12, Breeden, Sara wrote: > > > From: Breeden, Sara > Subject: [Histonet] Farewell > To: histonet@lists.utsouthwestern.edu > Date: Friday, March 30, 2012, 9:16 AM > > > Hard as it is to believe - and I probably won't until I've been away for > a week or more - this is my LAST day to work. Retirement? Who knew it > would come around so fast? I remember having only fifteen years left to > work and that was just yesterday! Histology has been 'bery, 'bery good > to me and although I fell into it by accident, it has been a > fascinating, involving, liberating experience. I'll be lurking on > Histonet but under the alias of nmhisto and I'll probably do some p.r.n. > work but I'm going home and throwing away my alarm clock and resetting > the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for > your advice, guidance, humor, relative insanity and wisdom. Enjoy what > you do and try to recruit at least one person into this field before > it's your time to pick out the color of the tennis balls on your walker. > > > > Hail and Farewell and best of everything to every one of you. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet