[Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates

[Smith, Allen]

I have used Vector's ImmPress and ABC secondary antibody systems.  I like ImmPress better: it has fewer steps, it gives me less background, and the reagents last longer.  Vector's Nova Red has given me better results than any other stain that I have tried.
-Allen A. Smith
Barry University School of Podiatric Medicine.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra
Sent: Wednesday, June 27, 2012 1:38 PM
To: 'Lewis, Patrick'; 'Histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates

We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit.

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Wednesday, June 27, 2012 9:45 AM
To: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates

Hi everyone,

I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates.

I love DAKO, but their kits and reagents are expensive.

Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits.

I do all my IHC manually by hand.  I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage.

Thanks

Patrick.

PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors.
      Has anyone tried Spring Bioscience's products?




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