[Histonet] RE: bone regrowth

[Elizabeth Chlipala]

Betsy

I would use formic acid rather than EDTA, especially if you need to run collagen II IHC, we ran into an issue with a study that was decaled in EDTA and the collagen II IHC was not as optimal with the EDTA decal, formic acid is much better for collagen II.  We also get good results for safranin O in most samples (except mouse joints) decaled in formic acid.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy
Sent: Friday, June 29, 2012 6:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: bone regrowth



From: Molinari, Betsy
Sent: Thursday, June 28, 2012 7:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: bone regrowth

Hi all,
I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences.
It involves bone regrowth. The sample will vary in size. From my reading, it seems that 5% formic  or 10% EDTA is preferred. The researcher asked about acetic acid, but I have never heard as acetic alone as a decal, isn't it just used to balance pH of the EDTA? It is my understanding that the decal step will be done somewhere else then the sample sent to me. That lab feels that HCL decal will not  not affect the sample. I think otherwise. The staining they have talked about is the Goldners Trichrome mainly. The researcher mentioned Safranin O but from my conversations I think they have little knowledge of histology and I work in a cardiovascular lab with my only experience has been with mouse tibia and these samples will be from a larger source.
Type 2 collagen was brought up but when I said that was an immuno stain they did not realize that.
Si in a nutshell, what decal sol. would be better and what stain?
Sorry for the rambling.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology





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Betsy Molinari
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