[Histonet] RE: Histonet Digest, Vol 103, Issue 31
Joshi, Neelam
NJoshi <@t> lsuhsc.edu
Tue Jun 26 14:22:06 CDT 2012
Hello Everybody,
My name is Neelam Joshi. I work for Louisiana State University Health Sciences Center. I am the supervisor of Histology and Immunohistochemistry Lab. I need the help to set the protocol to run HPV FAMILY16 low and high risk (ASR) on VENTANA XT intrument. The protocol which I have at present, having lot of background. My email address is njoshi <@t> lsuhsc.edu amd phone# 318-675-5874 Please let me know.
Thanks
Neelam Joshi ,HT(ASCP)
Histology Supervisor
Louisiana State University Health sciences Center
1501 Kings HWY-71130
Phone# 1-318-675-5874
njoshi <@t> lsuhsc.edu
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Tuesday, June 26, 2012 12:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 103, Issue 31
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Today's Topics:
1. Thank you to all who responded (Chiriboga, Luis)
2. (no subject) (manal galal)
3. Re: Embedding beads (Bob Richmond)
4. Re: Embedding beads (Alan Taylor)
5. macrophage stain (Galina Deyneko)
6. RE: Adopting a bar code system (Marcia Funk)
7. RE: RE: Adopting a bar code system (Clare Thornton)
8. RE: RE: Adopting a bar code system (Teri Johnson)
9. Tissue removal from slides (Jill Cox)
----------------------------------------------------------------------
Message: 1
Date: Mon, 25 Jun 2012 17:14:20 +0000
From: "Chiriboga, Luis" <Luis.Chiriboga <@t> nyumc.org>
Subject: [Histonet] Thank you to all who responded
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3E6798F00C9F494399E96B720ECD142908487F <@t> MSGWCDCPMB22.nyumc.org>
Content-Type: text/plain; charset="us-ascii"
To my Microm HM 500 OM User manual request!!
------------------------------
Message: 2
Date: Mon, 25 Jun 2012 11:15:52 -0700 (PDT)
From: manal galal <galalmkh <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: azzamonib <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu,
asmaa-twins <@t> hotmail.com, almasryeg <@t> yahoo.com
Message-ID:
<1340648152.15180.YahooMailNeo <@t> web130104.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii
http://apartment-in-jerusalem.com/ngler.html
------------------------------
Message: 3
Date: Mon, 25 Jun 2012 14:48:20 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Embedding beads
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH6qb67Yjq3dZHeM5TR1_brrdc=VNv2WQSCt9d+MSq7RXw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I've never seen beads used for identifying individual embedders by
marking blocks with the beads. I never heard of Hama Beads (that's how
it's spelled), which I think may be called Perler Beads in the US, but
I never heard of them either. I think you can get them from Amazon.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 4
Date: Mon, 25 Jun 2012 23:52:09 +0100
From: "Alan Taylor" <aj.taylor <@t> blueyonder.co.uk>
Subject: Re: [Histonet] Embedding beads
To: "Jennifer Campbell" <campbellj <@t> muhlbauerlab.com>, "Amber McKenzie"
<amber.mckenzie <@t> gastrodocs.net>
Cc: "Willis, Donna G." <Donna.Willis <@t> baylorhealth.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <79F573FCC6F94E63AB4E68A0E433FA39 <@t> merlin>
Content-Type: text/plain; charset="iso-8859-1"
Hi All
Thanks for the correction Dr Richmond. It is Hama Beads.
We keep ours in used rectangular cover slip boxes, which hold many beads of individual colours in each box. When in use each embedder has their allocated box at the back of the embedding centre, close to the dispensing spout, so that a bead can be quickly picked up with tweezers and dropped into the cassette tray when filling with wax.
When not in use the boxes are kept in a small instrument tray at the side of the embedding centre. Each persons name is clearly written on the lid of each box. The stock bags are kept in a nearby drawer.
Have never considered timing each embedding, but I am certain that there are very few additional seconds in reaching for a bead and dropping it into the molten wax.
Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. EX1 3AJ Devon. UK.
----- Original Message -----
From: Jennifer Campbell
To: Amber McKenzie
Cc: Alan Taylor ; Willis, Donna G. ; histonet <@t> lists.utsouthwestern.edu
Sent: Monday, June 25, 2012 2:51 PM
Subject: Re: [Histonet] Embedding beads
We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab.
There has to be accountability for every job.
Jen Campbell
On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
Does this slow the embedder down any having to pick a bead up each time to embed along with the tissue? I've never heard of this procedure before. The labs I've worked in have an embedding log and we initial each case we embed. What do you keep the beads in and are they set in a particular spot on the embedding station so they're easily assessable?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alan Taylor
Sent: Friday, June 22, 2012 5:15 PM
To: Willis, Donna G.; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Embedding beads
Hi All
We use Hamma beads to identify individual embedders on a particular day.
Hamma beads will be well known to parents and child carers as tiny tube like
beads that can be made into many patterns and then ironed (by adults) to
make colourful place mats etc for children.
Hamma beads come in a very wide range of colours, in bulk bags from any good
toy or handicraft store. They are very cheap and they are clearly visible
when placed in the corner of an embedding cassette when filling with wax.
We have a small staff in our lab so the colours we have chosen are readily
identifiable to an individual who has embedded a particular block. I can
strongly recommend them, we have used them for a number of years as a
traceable guide to a particular block. Hope this is helpful to all of you
who are embedding lots of blocks each day.
Best wishes to you all
Alan Taylor BSc(Hons), FRMS
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ.UK
----- Original Message -----
From: "Willis, Donna G." <Donna.Willis <@t> baylorhealth.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 22, 2012 3:58 PM
Subject: [Histonet] Embedding beads
Does anyone out in Histoland know of a vendor other that Mar Med to get
small beads to put in cassettes to designate the person that embeds.
Thanks,
Donna Willis, HT/HTL (ASCP)
Histology Lab Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.willis <@t> baylorhealth.edu
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--
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137
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------------------------------
Message: 5
Date: Tue, 26 Jun 2012 07:57:40 -0700 (PDT)
From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
Subject: [Histonet] macrophage stain
To: kimulah <@t> gmail.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1340722660.12041.YahooMailClassic <@t> web161003.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I use rat-a- mouse Mac3 on FFPE mouse aortic sinus. BD pharmigen # 550292,very simple basic protocol Dilution 1;200, no need HIER with tis dilution.
Galina Deyneko
Novartis, Cambridge, MA
?
617-871-7613 w
------------------------------
Message: 6
Date: Tue, 26 Jun 2012 10:58:48 -0400
From: "Marcia Funk" <FUNKM <@t> mercyhealth.com>
Subject: [Histonet] RE: Adopting a bar code system
To: "Teri Johnson" <TJohnson <@t> gnf.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Loralee A McMahon"
<Loralee_Mcmahon <@t> URMC.Rochester.edu>
Message-ID: <4FE987D8020000AC0001280F <@t> nodcdmg2.no.trinity-health.org>
Content-Type: text/plain; charset="us-ascii"
We are installing Vantage system from Ventana and very excited - great things to follow.
Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
>>> "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu> 06/21/2012 1:55 PM >>>
Sorry Teri must have missed the reply all button.
And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson <@t> gnf.org]
Sent: Thursday, June 21, 2012 2:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Adopting a bar code system
Here's a response I have received off list:
Teri,
We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc.
I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson <@t> gnf.org]
Sent: Thursday, June 21, 2012 1:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Adopting a bar code system
Hi Histonetters,
I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so.
Thanks!
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 7
Date: Tue, 26 Jun 2012 11:30:05 -0400
From: Clare Thornton <CThornton <@t> dahlchase.com>
Subject: RE: [Histonet] RE: Adopting a bar code system
To: 'Marcia Funk' <FUNKM <@t> mercyhealth.com>, Teri Johnson
<TJohnson <@t> gnf.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Loralee A McMahon
<Loralee_Mcmahon <@t> URMC.Rochester.edu>
Message-ID:
<C9D78FFC9D668B4CBEA4405F846975040109B4F735E3 <@t> iris.dahlchase.net>
Content-Type: text/plain; charset="us-ascii"
If you will be at NSH in Vancouver, I will be presenting a workshop called "Every Step You Take: Implementing a Tracking System in the Pathology Laboratory". I presented this at the Region I meeting in NY this past April and received good feedback that the information was useful. It is scheduled for Saturday afternoon. I do concentrate on my particular lab's tracking system (while making no recommendations) but talk in depth about implementation, added values, and pros and cons.
Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia Funk
Sent: Tuesday, June 26, 2012 10:59 AM
To: Teri Johnson; histonet <@t> lists.utsouthwestern.edu; Loralee A McMahon
Subject: [Histonet] RE: Adopting a bar code system
We are installing Vantage system from Ventana and very excited - great things to follow.
Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
>>> "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu> 06/21/2012 1:55 PM >>>
Sorry Teri must have missed the reply all button.
And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson <@t> gnf.org]
Sent: Thursday, June 21, 2012 2:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Adopting a bar code system
Here's a response I have received off list:
Teri,
We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc.
I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson <@t> gnf.org]
Sent: Thursday, June 21, 2012 1:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Adopting a bar code system
Hi Histonetters,
I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so.
Thanks!
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 26 Jun 2012 15:39:36 +0000
From: Teri Johnson <TJohnson <@t> gnf.org>
Subject: RE: [Histonet] RE: Adopting a bar code system
To: Clare Thornton <CThornton <@t> dahlchase.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9F3CFEE76E51B64991C7485270890B400CDBEEED <@t> EX4.lj.gnf.org>
Content-Type: text/plain; charset="us-ascii"
Clare, I have already chosen this workshop and I will be attending it. Thank you for this email.
Teri
-----Original Message-----
From: Clare Thornton [mailto:CThornton <@t> dahlchase.com]
Sent: Tuesday, June 26, 2012 8:30 AM
To: 'Marcia Funk'; Teri Johnson; histonet <@t> lists.utsouthwestern.edu; Loralee A McMahon
Subject: RE: [Histonet] RE: Adopting a bar code system
If you will be at NSH in Vancouver, I will be presenting a workshop called "Every Step You Take: Implementing a Tracking System in the Pathology Laboratory". I presented this at the Region I meeting in NY this past April and received good feedback that the information was useful. It is scheduled for Saturday afternoon. I do concentrate on my particular lab's tracking system (while making no recommendations) but talk in depth about implementation, added values, and pros and cons.
Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia Funk
Sent: Tuesday, June 26, 2012 10:59 AM
To: Teri Johnson; histonet <@t> lists.utsouthwestern.edu; Loralee A McMahon
Subject: [Histonet] RE: Adopting a bar code system
We are installing Vantage system from Ventana and very excited - great things to follow.
Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
>>> "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu> 06/21/2012 1:55 PM >>>
Sorry Teri must have missed the reply all button.
And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson <@t> gnf.org]
Sent: Thursday, June 21, 2012 2:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Adopting a bar code system
Here's a response I have received off list:
Teri,
We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc.
I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson <@t> gnf.org]
Sent: Thursday, June 21, 2012 1:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Adopting a bar code system
Hi Histonetters,
I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so.
Thanks!
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 26 Jun 2012 08:49:32 -0700 (PDT)
From: Jill Cox <jcox90 <@t> yahoo.com>
Subject: [Histonet] Tissue removal from slides
To: "Histonet <@t> Lists. Edu" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1340725772.80693.YahooMailNeo <@t> web161606.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Histoland,
?Does anyone know the name of the reagent that is used to remove or split tissue sections from slides? It pours on like liquid and allows you to peel off after a couple of hours, it's been years since I've done this so don't remember.. Thanks in advance!!
?
Jill Cox HT (ASCP)
Pathology Lab/ Histologist
Cancer Treatment Centers of America
Ph: 623-207-3710 F: 623-207-3717
------------------------------
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End of Histonet Digest, Vol 103, Issue 31
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