[Histonet] RE: Muscle Biopsy Procedure

[Sarah Dysart]

I have done the same thing with acetone instead of alcohol...

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Monday, June 25, 2012 10:33 AM
To: Bea DeBrosse-Serra; 'Ian R Bernard'; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Muscle Biopsy Procedure

One place I was at didnt get many muscle biopsies so we didnt keep liquid nitrogen and isopentane, which is what I was trained to use initially. Here's what we did when we got a muscle biopsy and it worked fine. 
 
After cutting the biopsy into 3-4 mm portions, we wrapped it in aluminum foil and snap froze it in a slush made from 100% alcohol and dry ice. We then shipped it in a styrofoam lined box full of peices of dry ice. The dry ice was made into small peices so we could place the snap frozen biopsy in the middle. 
 
Good luck! 


________________________________
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
To: 'Ian R Bernard' <ibernard <@t> uab.edu>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, June 25, 2012 10:16 AM
Subject: [Histonet] RE: Muscle Biopsy Procedure

Ian, 

Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. 

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian R Bernard
Sent: Friday, June 22, 2012 7:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Muscle Biopisy Procedure

Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one.  Since we never did one, we don't have a written procedure.

We are looking for a  benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing.

We will not perform any other aspects of the procedure, just the freeze and ship.

Hope to do this next week.  Any assistance is greatly appreciated.

Ian R. Bernard
Ian R. Bernard, MSHA, HT (ASCP)
10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840

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