[Histonet] Eosin staining for small biopsies

[Cynthia Pyse]

We also use safranin on all our small biopsies and also our breast biopsies
with no adverse effects to any of our IHC or ISH.
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cpyse <@t> x-celllab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson
Sent: Wednesday, June 20, 2012 10:32 AM
To: histonet <@t> lists.utsouthwestern.edu; Carol Freeman
Subject: Re: [Histonet] Eosin staining for small biopsies

We use safranin (used in Microbiology as a counterstain)  on our small
biopsies. We apply a small drop during grossing. It does not affect staining
of H&E, IHC or ISH. We like this because it is an intense red that doesn't
leach out in the alcohols of processor.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc <@t> mercyhealth.com


>>> "Freeman, Carol" <Carol.Freeman <@t> utoledo.edu> 6/20/2012 8:46 AM >>>


Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First does
anyone have any papers written or studies done on the use of Eosin to stain
small biopsies or the use of eosin on the tissue processor in the last
alcohol??  I have read snippets on eosin having an effect on FISH testing??
Does anyone know of this to be true and have any papers or studies to refer
back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies
and/or any thoughts on its use to do so?? My thoughts are that because of
it's precipitation qualities it could gunk up the tissue processor and leave
residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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