[Histonet] RE: Eosin staining for small biopsies

[Blazek, Linda]

We use eosin in our last 100% alcohol on the processor.  It is a great help for our person embedding to be able to see and orient the small biopsies.  We have been doing this for years and have never had any problem with the processor or any subsequent staining that has been performed.  We also at times ink translucent esophageal biopsies at the grossing station to help in embedding.  This also has never presented a problem. 

Linda

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Freeman, Carol
Sent: Wednesday, June 20, 2012 9:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin staining for small biopsies



Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol??  I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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