[Histonet] RE: Antigen retrieval

[pruegg <@t> ihctech.net]

   The  most  important  part   known  positive  tissue  to use as   everything  you  do is a shot in the dark   protocol  is  not  working  or  the  control  you  are     expressing that antigen.  Each antibody has it's own requ   pretreatment or not.  We do what Liz does and sometimes ad   buffer  when  nothing  else works.  Just to save on slides to    start  with no ar and ph6 and if we do not get good results, go to ed   ta  ph9,  then PK, then ph8, if all these fail, we revert to overnight
   incuba   buffers and/or enzy   all  of  course  a   Some of my experien   nuclei  may  prefer  high  ph   noticed, definitely not something I h
   Regards,<
   Patsy


     


   
-------- Original Message --------
   
Subject: [Histonet] RE: Antigen retrieval
   
From: Elizabeth Chlipal      
To: 'Mike Tighe' <[2]mtighe <@t> trudeauinstitute.org>,
      istonet <@t> lists.utsouthwestern.edu)"
   
<[5]histonet <@t> lists.utsouthwestern.edu>
   
   
Mike
   

   
Retrieval  methods  are  also  based  upon  the  antibody   protocol  development we try several different retrieval methods -   retrieval,  enzymatic  (proteinase  K), pH6 and pH9, we then determine
   the   for all    

   
Liz
   

   
Elizabeth A. Chlipala, BS, HTL(AS   
Manager
   
Premier Laboratory, LLC
   
PO Box 18592
      
(303) 682-3949 office
   
(303) 682-9060    
(303) 881-0763 cell
   
[6]w   

   
Ship to address:
   

   
1567 Skywa   
Longmont, CO 80504
   

   
-----Original Message   
From:                  [7]histonet-bounces <@t> lists.utsouthwestern.edu
   [[8]mailto:histonet-bounces <@t> lists.utsouthw   Mike Tighe
   
Sent: Wednesday, June 13, 201   
To:              [9]hi   ([10]histonet <@t> lists.utsouthwestern.edu)
   
Subject: [Histo   

   
Does  anyone  have  a  favorite antigen ret   mouse  tissues that they would be willing to share? I   citrate  buffer  Ph6.0  with poor to moderate results. Thanks   help!
   

   

   

   
Mike
   
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References

   1. 3D"mailto:liz <@t> premierlab.com"
   2. file://localhost/tmp/3D"m   3. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   4. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   5. 3D"mailto:histonet   6. 3D"http://www.premierlab.com"/
   7. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.e   8. 3D"mailto:histon   9. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
  10. 3D"mailto:histonet <@t> lists.utsou  11. file://localhost/tmp/3D"mail  12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet  13. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
  14. 3D"http://lists.utsouthwestern.edu/