[Histonet] Sequenza units for manual immunohistochemistry

[Beckham, Sharon]

I have used them for quite some time.  I was having problems with uneven staining and determined that it was from the being too full and was drawing up liquid from the bottom.  There is a small opening on the end and I just empty the solution from it.  I usually empty it after rinsing the antibody off and then usually before putting DAB or DAPI on depending on the number of slides stained.  As for washing, I just wash in a solution of soapy water and then rinse in distilled.  I use the coverplates for several staining times.  I love the sequenza and prefer it to having an automatic stainer.
 


Sharon Beckham, HT (ASCP)
Head, Histotechnology
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
(816)926-4305
slb <@t> stowers.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: Wednesday, June 06, 2012 12:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sequenza units for manual immunohistochemistry

Hello Histonet,

I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent "waste" in the bottom from all your additions and washes.  Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom.

Has anyone had any issues using these units?  How do people prevent a large volume from building up in the bottom?  How are people cleaning their coverplates and the units themselves?

Thanks in advance for your thoughts!


Keri

Keri Colwell, BSc
Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640  | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell <@t> inspection.gc.ca Telephone | Téléphone:  403-382-5500 ext 5613/5511 Facsimile | Télécopieur: 403-382-5585 Government of Canada | Gouvernement du Canada

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