[Histonet] Fluorescence-filters

[Anatoli Gleiberman]

Filters are usually very stable. What is burning out are fiber optic cables that are present in some illumination systems such as X-Cite in Zeiss microscopes. These optical fiber cables should be replaced after 2000 hours.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, June 05, 2012 10:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fluorescence-filters

Hi!

Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year.

 

What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. 

 

Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.

 

Hoping for responses

Gudrun Lang

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


This communication may contain privileged information.  It is intended solely for the use of the addressee.  If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information.  If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.  This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.