From Susan.Walzer <@t> HCAHealthcare.com Fri Jun 1 03:42:42 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Jun 1 03:42:54 2012 Subject: [Histonet] Uranyl nitrate Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DEC219768@FWDCWPMSGCMS09.hca.corpad.net> Does anyone know a retic stain that does not use uranyl nitrate? From W.E.J.Hoekert <@t> olvg.nl Fri Jun 1 06:42:37 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Jun 1 06:42:48 2012 Subject: [Histonet] MDM-2 on Ventana, which clone? References: <1338325100.66322.YahooMailNeo@web39402.mail.mud.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F8424B@PAIT42.olvg.nl> Hi Histonetters, I am having a very hard time in setting up the MDM-2 antibody on our Ventana Benchmark XT's. I am using clone SMP14 from Becton Dickinson, which was working fine on the Labvision. I have tried everything (even incubating overnight at 4?C) and I am out of idea's. Maybe I should use another clone, for instance 1B10 from Monosan? Can somebody please give me a good protocol? Thank's in advance, Willem Hoekert Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From one_angel_secret <@t> yahoo.com Fri Jun 1 06:50:14 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jun 1 06:50:59 2012 Subject: [Histonet] floaters - thisisann@aol.com In-Reply-To: <1338325100.66322.YahooMailNeo@web39402.mail.mud.yahoo.com> References: <1338325100.66322.YahooMailNeo@web39402.mail.mud.yahoo.com> Message-ID: <6C4645EA-8C37-44F7-8C1B-6302121BC0B4@yahoo.com> Beautiful reply! Sent from my iPhone On May 29, 2012, at 4:58 PM, Cheryl wrote: > Hi Ann- > > May we assume you've confirmed this is happening at embedding and have ruled out any floaters happening during cutting? > > When embedding, keep Kimwipes or other tissues around, keep the wells closed and only pull out enough cassettes that you can keeep clean and clear of your working area. Wipe forceps and surfaces between blocks containing fragmented or friable tissue, don't put forceps back in the wells without wiping. You can stack a few guaze pads on top of the spout to wipe as you replace the forceps and change the pads frequently. Always, always only open one cassettte at a time and never leave the station with an open cassette on the station. Finish before standing or recap and replace. > > If you are working with currettes, cell blocks, or other cellular, friable tissue, open and unwrap on a clean surface (hot or cold - embedding station surface or wipe or l'absorb) and don't reuse the surface before wiping or replacing. If you're using knives or scalpels to scrape, make sure the handles and connection points aren't harboring residual tissue. Buy those little seamless paring knive from the dollar store--they fit in the wells and wipe easily. Use swabs between embedding sessions or between people trading places to clean the wells and then clean them again at the end of the embedding session. > > If you keep molten paraffin in the hold bins, filter or replace frequently and do not reuse. If you keep the hold bins dry, clean routinely (daily) Clean your molten wax chamber periodically to remove contaminants and keep the filter from clogging over time. Most embedding station mfc don't condone running xylene through the tubes & pumps--clean hot wax will do the job. > > We always make it the responsibility of each person to clean both at the end of embedding AND to clean again before starting to fully assure the wells and surfaces were clear and eliminate a possible cross if one person in the chain forgets...double system processes like you double check specimen IDs. > > You'll go through a whole bunch of kimwipes--but they are much cheaper than gauze and SOOO much better than a cross contamination situation. IF it still happens, it's time to track who embeds each block to see if there's a pattern by person. The point is not to write people up but to support developing clean habits and to adjust their habits to do it to their best ability. > > Wipe wipe wipe wipe wipe!! Hope this helps! > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Fri Jun 1 08:30:05 2012 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Jun 1 08:30:18 2012 Subject: [Histonet] Block Alignment Tool Message-ID: <5F06C3AD0B27264CA20CFA986C87882E3149C3CE@EXCHANGEPV1.KGH.ON.CA> Interesting thread about block alignment. Bernice makes a good point about Newcomer's level, and the possible effects of benchtop or floor not being level; perhaps the stage-mounted tools are preferable. We have a stage-mounted tool, which brings me to another point. Before this type of tool was invented, we used a block of wax, no tissue, for alignment. This required some eye-balling and often subsequent adjustment of the head. This was a skill learned by experience and practice, as most skills are. The results were usually good. Now that we have Leica RM2255 motorized microtomes, I find their method of aligning the block to be excellent, with red cylinders showing aligned or not on both axes. We receive many cases from referring hospitals for subsequent IHC in our laboratory. Many of these blocks contain very thin biopsies which have already been cut. At this point, when microtomy for IHC begins, the alignment almost inevitably has to be adjusted. This takes us back to the eye-balling mentioned earlier, and very careful initial turns of the wheel to gauge proper alignment and not waste tissue. But in terms of daily microtomy of just-embedded blocks, either the Leica microtome method or the block alignment tool will go a long way to ensuring that all microtomes are as closely aligned as possible. They all rely on proper training and experience to ensure this. Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada From gagnone <@t> KGH.KARI.NET Fri Jun 1 08:31:04 2012 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Jun 1 08:31:24 2012 Subject: [Histonet] Friday funny...block alignment Message-ID: <5F06C3AD0B27264CA20CFA986C87882E3149C3DA@EXCHANGEPV1.KGH.ON.CA> Speaking of block alignment, when this topic comes up, someone invariably pipes up... "Hey, is your head on straight?" Eric From Carol.Freeman <@t> utoledo.edu Fri Jun 1 08:29:42 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Fri Jun 1 08:32:33 2012 Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 40 In-Reply-To: References: Message-ID: I find it sad that there are so many messages to unsubscribe following the great certification debacle that has been on here the last couple of days. I personally just find it tedious that on a server set up purely for histology is where we are discussing whether or not histology is a respectable profession. I would like to believe it is the ones sharing their nasty viewpoints and lack of self worth, but sadly it is probably some of the good ones that we have lost. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 31, 2012 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 102, Issue 40 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. PathCentral LIS (Bull, Jennifer L.) 2. Unsubscribe (Blundon, Kimberly) 3. CoPath Users using Dragon Software (Mike Pence) 4. RE: QIHC (joelle weaver) 5. Please unsubscribe (Yvonne) 6. Unsubscribe (Blundon, Kimberly) 7. RE: compatibility of Thermo slide printers with Leica cassette printer (joelle weaver) 8. RE: CoPath Users using Dragon Software (Inman, Anna) 9. EPIC Beaker AP or clinical system (Jessica.Vacca@HCAhealthcare.com) 10. How to Unsubscribe, Part 114 (nmhisto@comcast.net) 11. Masson trichrome goldner (prisana.p@psu.ac.th) 12. Re: Masson trichrome goldner (Morphisto GmbH) 13. RE: How to Unsubscribe, Part 114 (Sherwood, Margaret) 14. Please unsubscribe (Bella Shmaltsuyeva) 15. RE: Please unsubscribe (Connolly, Brett M) 16. please unsubscribe (Debbie Taylor) 17. RE: please unsubscribe (WILLIAM DESALVO) 18. ADH 5 (Rae Staskiewicz) 19. RE: please unsubscribe (Bartlett, Jeanine (CDC/OID/NCEZID)) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 May 2012 10:07:08 -0700 From: "Bull, Jennifer L." Subject: [Histonet] PathCentral LIS To: "histonet@lists.utsouthwestern.edu" Message-ID: <85760CECEC18444BB95F26D5E88DAEAA2396472321@hinet2.hinet.org> Content-Type: text/plain; charset=us-ascii Are there any labs out there that are currently using PathCentral as an LIS? Any feedback is appreciated. Thanks! mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Wed, 30 May 2012 11:11:30 -0600 From: "Blundon, Kimberly" Subject: [Histonet] Unsubscribe To: Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501EFE451@suffieldex01.suffield.drdc-rddc.gc.ca> Content-Type: text/plain; charset="iso-8859-1" Unsubscribe please. Thank you. Kimberly Blundon B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.blundon@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada ------------------------------ Message: 3 Date: Wed, 30 May 2012 12:15:39 -0500 From: "Mike Pence" Subject: [Histonet] CoPath Users using Dragon Software To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E34@is-e2k3.grhs.net> Content-Type: text/plain; charset="US-ASCII" I am looking for anyone that is using CoPath Sunquest and using Dragon software at gross and by the pathologist. Also what are you using for hands-free dictation at gross and at sign out? You can contact me offline if you wish. Thanks, Mike ------------------------------ Message: 4 Date: Wed, 30 May 2012 17:20:49 +0000 From: joelle weaver Subject: RE: [Histonet] QIHC To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I used the Dako guides, there is a good theory one and also an AR guide. That is about all I used to study. They are on their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: raestask@grics.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 30 May 2012 09:35:43 -0500 > Subject: [Histonet] QIHC > > Is there any other resource for information and studying for the QIHC exam > other than the ASCP outline? > > > > Rae Staskiewicz > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 30 May 2012 13:34:25 -0400 From: Yvonne Subject: [Histonet] Please unsubscribe To: "Histonet@lists.utsouthwestern.edu" Message-ID: <76C45466-611D-481D-85C1-26CED4FA7D3B@gmail.com> Content-Type: text/plain; charset=us-ascii Please unsubscribe me from this list. Sent from my iPod ------------------------------ Message: 6 Date: Wed, 30 May 2012 11:51:13 -0600 From: "Blundon, Kimberly" Subject: [Histonet] Unsubscribe To: Message-ID: <42DFE1A029181B4B8CCBA7261B52D76501EFE452@suffieldex01.suffield.drdc-rddc.gc.ca> Content-Type: text/plain; charset="iso-8859-1" Please unsubscribe me from this list. Thank you Kimberly Blundon B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.blundon@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada ------------------------------ Message: 7 Date: Wed, 30 May 2012 17:52:55 +0000 From: joelle weaver Subject: RE: [Histonet] compatibility of Thermo slide printers with Leica cassette printer To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have used the code printed cassettes, but with QR codes. I have seen the slide printing only but not in use (yet). In theory any reader should be able to read the standard symbologies. But since they are different vendors, you might contact them directly about compatability for transfer of patient ID data. Maybe middleware is an option if they are not directly compatible. ,Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 29 May 2012 15:12:07 -0500 > From: Sandra.Harrison3@va.gov > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] compatibility of Thermo slide printers with Leica cassette printer > > 1) Does anyone have a Leica Cassette Printer on which they are > printing 2-Dimensional barcodes? > > > > 2) Is anyone using a Thermo slide printer, with bar code reader, to > read cassettes printed off of a Leica Cassette Printer with 2-D bar > codes? > > > > Thanks for your input. > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 30 May 2012 12:23:22 -0600 From: "Inman, Anna" Subject: RE: [Histonet] CoPath Users using Dragon Software To: "Mike Pence" , Message-ID: <2925AE271EAAD440AF48FCCEB8002D091C0778A9@smgmail01.smgj.sclhs.net> Content-Type: text/plain; charset="us-ascii" I would be interested in this info, as well - please share! Thank you Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, May 30, 2012 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CoPath Users using Dragon Software I am looking for anyone that is using CoPath Sunquest and using Dragon software at gross and by the pathologist. Also what are you using for hands-free dictation at gross and at sign out? You can contact me offline if you wish. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Wed, 30 May 2012 13:35:15 -0500 From: Subject: [Histonet] EPIC Beaker AP or clinical system To: Message-ID: <938D716CD445614ABBB817517557B6F4076FA3FD5F@NADCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Is anyone out there on the Epic-Beaker system? I would like to talk to someone that is already live on the system, if you have a few minutes can you contact me at the email below? Thanks Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 813-789-3588 e: Jessica.Vacca@hcahealthcare.com ------------------------------ Message: 10 Date: Wed, 30 May 2012 22:00:30 +0000 (UTC) From: nmhisto@comcast.net Subject: [Histonet] How to Unsubscribe, Part 114 To: HISTONET Message-ID: <1688184558.484887.1338415230136.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Okay, I'm retired now but ??I monitor Histonet from my dining room table while eating bon-bons between soap operas.?? Yeah, right.?? But that doesn't mean I can't add to the conversation... I would like to remind all subscribers (for the 114th time) that when you subscribed to Histonet, you were given ?? UNSUBSCRIBE directions . The listserv manager cannot unsubscribe you - you must do it yourself.?? Does it sound like I've mellowed any in the last 60 days??? Not!?? I miss my microtome and hope that Carole is talking nicely to it as I did... ------------------------------ Message: 11 Date: Thu, 31 May 2012 10:05:31 +0700 (ICT) From: prisana.p@psu.ac.th Subject: [Histonet] Masson trichrome goldner To: histonet@lists.utsouthwestern.edu Message-ID: <9050cb3bd212273eb67f0951c4222f0e.squirrel@webmail.psu.ac.th> Content-Type: text/plain;charset=tis-620 Dear I am working on undecalcified section of bone and stain it with masson trichrome goldner. I am not sure aabout the protocol I used or anything wrong because all my bone is red not green. Does anyone has the same problem? Please suggest me what happen and what to do. if you could share the protocol it will be very appreciated. yours sincerely Prisana ------------------------------ Message: 12 Date: Thu, 31 May 2012 07:15:54 +0200 From: Morphisto GmbH Subject: Re: [Histonet] Masson trichrome goldner To: prisana.p@psu.ac.th, Histonet@lists.utsouthwestern.edu Message-ID: <458F586C-B407-4803-999E-91A1A9DC3C57@morphisto.de> Content-Type: text/plain; charset=iso-8859-1 Dear Prisana, we often stain bones and other similiar tissues with masson goldner, but we never had the problem, that the bone becomes red instead of green or blue (depending if you use light green or aniline blue). We use the protocol which you can find here in this list: http://www.morphisto.de/en/histology/methods/ The protocol and the names of the solutions are in german, but I think it will be no problem, to understand it. Best regards Michael Am 31.05.2012 um 05:05 schrieb prisana.p@psu.ac.th: > Dear > I am working on undecalcified section of bone and stain it with masson > trichrome goldner. I am not sure aabout the protocol I used or anything > wrong because all my bone is red not green. Does anyone has the same > problem? Please suggest me what happen and what to do. if you could share > the protocol it will be very appreciated. > yours sincerely > Prisana > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 60 Telefax: 069 / 989 72 1100 E-Mail: info@morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. ------------------------------ Message: 13 Date: Thu, 31 May 2012 13:55:21 +0000 From: "Sherwood, Margaret" Subject: RE: [Histonet] How to Unsubscribe, Part 114 To: "'nmhisto@comcast.net'" , HISTONET Message-ID: <090FA56107A969459F3941DDD5585C3A1175F6D8@PHSX10MB10.partners.org> Content-Type: text/plain; charset="iso-8859-1" I love it! How true. I get frustrated as well. Enjoy your retirement! Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Wednesday, May 30, 2012 6:01 PM To: HISTONET Subject: [Histonet] How to Unsubscribe, Part 114 Okay, I'm retired now but ?I monitor Histonet from my dining room table while eating bon-bons between soap operas.? Yeah, right.? But that doesn't mean I can't add to the conversation... I would like to remind all subscribers (for the 114th time) that when you subscribed to Histonet, you were given ? UNSUBSCRIBE directions . The listserv manager cannot unsubscribe you - you must do it yourself.? Does it sound like I've mellowed any in the last 60 days?? Not!? I miss my microtome and hope that Carole is talking nicely to it as I did... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 14 Date: Thu, 31 May 2012 15:13:58 +0000 From: Bella Shmaltsuyeva Subject: [Histonet] Please unsubscribe To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Please unsubscribe me from this list. Bella Shmaltsuyeva, HT (ASCP),QIHC Senior Research Tech Pathology Core Facility Robert H Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-429 Chicago ,IL6061 312-503-4705 b-shmaltsuyev@northwestern.edu ------------------------------ Message: 15 Date: Thu, 31 May 2012 11:18:23 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: Please unsubscribe To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" # 115 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bella Shmaltsuyeva Sent: Thursday, May 31, 2012 11:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Please unsubscribe Please unsubscribe me from this list. Bella Shmaltsuyeva, HT (ASCP),QIHC Senior Research Tech Pathology Core Facility Robert H Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-429 Chicago ,IL6061 312-503-4705 b-shmaltsuyev@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 16 Date: Thu, 31 May 2012 11:34:52 -0400 From: "Debbie Taylor" Subject: [Histonet] please unsubscribe To: Message-ID: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> Content-Type: text/plain; charset="us-ascii" Please unsubscribe me from this list. Thanks. Deborah Taylor, MS Customer Relations/Lab Manager Marlboro Chesterfield Pathology, PC 672 Hwy 9 West Bennettsville, SC 29512 Phone: 843-479-2402 Fax: 843-479-6609 Note: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anone else is unauthorized. If you are not the intended recipient, any disclosure copying or distribution of the message or any action or omission taken by you in reliance on it, is prohibitied and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. ------------------------------ Message: 17 Date: Thu, 31 May 2012 09:40:06 -0600 From: WILLIAM DESALVO Subject: RE: [Histonet] please unsubscribe To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" #116 - WHEN WILL IT STOP????? William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 31 May 2012 11:34:52 -0400 > From: dtaylor@mcpathology.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] please unsubscribe > > Please unsubscribe me from this list. Thanks. > > > > Deborah Taylor, MS > > Customer Relations/Lab Manager > > Marlboro Chesterfield Pathology, PC > > 672 Hwy 9 West > > Bennettsville, SC 29512 > > Phone: 843-479-2402 > > Fax: 843-479-6609 > > > > > > Note: The information in this message is confidential and may be > legally privileged. It is intended solely for the addressee. Access to > this message by anone else is unauthorized. If you are not the intended > recipient, any disclosure copying or distribution of the message or any > action or omission taken by you in reliance on it, is prohibitied and > may be unlawful. Please immediately contact the sender if you have > received this message in error. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 31 May 2012 11:40:54 -0400 From: "Rae Staskiewicz" Subject: [Histonet] ADH 5 To: Histonet@lists.utsouthwestern.edu Message-ID: <20120531114054.ru0s93ll70n408g4@webmail2.centurytel.net> Content-Type: text/plain; charset=UTF-8 Is anyone doing the ADH 5 stain on a Ventana platform? Rae Staskiewicz Methodist Medical Center in Illinois ------------------------------ Message: 19 Date: Thu, 31 May 2012 15:41:10 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] please unsubscribe To: WILLIAM DESALVO , "dtaylor@mcpathology.com" , histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Too funny! Everyone, UNSUBSCRIBE YOURSELF! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, May 31, 2012 11:40 AM To: dtaylor@mcpathology.com; histonet Subject: RE: [Histonet] please unsubscribe #116 - WHEN WILL IT STOP????? William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 31 May 2012 11:34:52 -0400 > From: dtaylor@mcpathology.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] please unsubscribe > > Please unsubscribe me from this list. Thanks. > > > > Deborah Taylor, MS > > Customer Relations/Lab Manager > > Marlboro Chesterfield Pathology, PC > > 672 Hwy 9 West > > Bennettsville, SC 29512 > > Phone: 843-479-2402 > > Fax: 843-479-6609 > > > > > > Note: The information in this message is confidential and may be > legally privileged. It is intended solely for the addressee. Access to > this message by anone else is unauthorized. If you are not the > intended recipient, any disclosure copying or distribution of the > message or any action or omission taken by you in reliance on it, is > prohibitied and may be unlawful. Please immediately contact the sender > if you have received this message in error. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 102, Issue 40 ***************************************** From b-frederick <@t> northwestern.edu Fri Jun 1 08:38:02 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 1 08:38:09 2012 Subject: [Histonet] RE: Friday funny...block alignment In-Reply-To: <5F06C3AD0B27264CA20CFA986C87882E3149C3DA@EXCHANGEPV1.KGH.ON.CA> References: <5F06C3AD0B27264CA20CFA986C87882E3149C3DA@EXCHANGEPV1.KGH.ON.CA> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E254DA8@evcspmbx3.ads.northwestern.edu> Haven't had that checked lately. How about you? I know my mom may have had something to say to that effect back in high school...... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Friday, June 01, 2012 8:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday funny...block alignment Speaking of block alignment, when this topic comes up, someone invariably pipes up... "Hey, is your head on straight?" Eric _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri Jun 1 09:29:55 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Jun 1 09:30:10 2012 Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 40 In-Reply-To: References: Message-ID: I personally found the discussions on certification enlightening and occasionally entertaining. The opinions expressed are simply opinions - I do not judge them as "nasty" or "tedious". If I am not interested in a discussion thread, I simply do not read it. tg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol Sent: Friday, June 01, 2012 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 40 I find it sad that there are so many messages to unsubscribe following the great certification debacle that has been on here the last couple of days. I personally just find it tedious that on a server set up purely for histology is where we are discussing whether or not histology is a respectable profession. I would like to believe it is the ones sharing their nasty viewpoints and lack of self worth, but sadly it is probably some of the good ones that we have lost. From Jessica <@t> nsh.org Fri Jun 1 09:31:20 2012 From: Jessica <@t> nsh.org (Jessica Smith) Date: Fri Jun 1 09:31:32 2012 Subject: [Histonet] NSH Troubleshooting Stains Forum Message-ID: <2B6E973D7D12964F8D8A3598557C553008E4B5@NSH-SRVR01.nsh.local> Join The National Society for Histotechnology in Orlando, FL this August for a One-Day Forum on Troubleshooting Stains! NSH has recruited two of its highest rated speakers to bring you this one day forum where attendees will learn good practices for both H&E and IHC Staining. The morning provides an opportunity for you to learn if your "gold standard" H&E is living up to expectations. Then spend your afternoon learning how to optimize IHC regardless of the fixative, antibody or protocol used. When: Saturday, August 4, 2012 from 8:00am-4:30pm Where: Doubletree by Hilton Orlando at SeaWorld Visit: http://s3.goeshow.com/nsh/StainsE12/ereg875669.cfm?pg=home for all the details regarding Travel, Schedule, and Registration! Contact histo@nsh.org for questions or give NSH a call at 443.535.4060. Jessica Smith Meeting Coordinator/Social Media Specialist National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From b-frederick <@t> northwestern.edu Fri Jun 1 09:40:58 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 1 09:41:07 2012 Subject: [Histonet] RE: CAP In-Reply-To: References: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E254E03@evcspmbx3.ads.northwestern.edu> We got an A+ rating......... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Wednesday, May 30, 2012 9:34 AM To: Bernice Frederick; Fellow HistoNetters Subject: RE: CAP Have fun and good luck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, May 30, 2012 7:16 AM To: Fellow HistoNetters Subject: [Histonet] CAP They're here!!!!!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Wanda.Smith <@t> HCAhealthcare.com Fri Jun 1 10:27:33 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Jun 1 10:27:39 2012 Subject: [Histonet] RE: CAP In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E254E03@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E253966@evcspmbx3.ads.northwestern.edu> <62C639732D3F274DACED033EBDF6ADAF1E254E03@evcspmbx3.ads.northwestern.edu> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27414D6D9A@NADCWPMSGCMS03.hca.corpad.net> Congratulations!!!!!! WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, June 01, 2012 10:41 AM To: Jesus Ellin; Fellow HistoNetters Subject: [Histonet] RE: CAP We got an A+ rating......... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Wednesday, May 30, 2012 9:34 AM To: Bernice Frederick; Fellow HistoNetters Subject: RE: CAP Have fun and good luck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, May 30, 2012 7:16 AM To: Fellow HistoNetters Subject: [Histonet] CAP They're here!!!!!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Jun 1 10:45:06 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Jun 1 10:45:18 2012 Subject: [Histonet] RELIA Solutions HOT Histology Job Alert. Histology Tech HT/HTL needed for a brand new lab in Eastern TN Message-ID: <006b01cd400d$8525ec60$8f71c520$@earthlink.net> Hi Histonetters! I hope you area having a great Friday and looking forward to a fun filled weekend. I have a new position that I want to tell you about. I am working with a lab in eastern TN that is setting up a brand new histology lab! This is a permanent full time day shift position and my client offers an excellent salary and great benefits. The ideal candidate will be ASCP HT/HTL certified and CLIA qualified to gross with at least 3 years of histology experience. Since this is a brand new lab there is a lot of potential for growth. If you are interested in hearing more about it for yourself please contact me right away. If you know someone who might be interested please fill them in and have them contact me - If I place them I will pay you a referral fee. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From madeleinehuey <@t> gmail.com Fri Jun 1 12:28:30 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Jun 1 12:28:35 2012 Subject: [Histonet] hiring - writing SOP policies (Histology & IPOX) Message-ID: Hello Histonetters, We are looking for someone who have a lot of experience in writing SOP policies for Histology & IHC (ie.CAP check-list). You can do them at home (if your retiree) or in our lab. We will pay your service. If you know someone who might be interested please tell them to contact me as well. My contact number 650-940-7038 or 415/572-3013. madeleinehuey@elcaminohospital.org From sprice2003 <@t> gmail.com Fri Jun 1 12:29:08 2012 From: sprice2003 <@t> gmail.com (Sally Price) Date: Fri Jun 1 12:29:13 2012 Subject: [Histonet] In FL, who can perform IHC/ISH? Message-ID: I realize that this is a controversial topic, bu t I'm looking for a definitive answer to the question of who can perfrom IHC and ISH in Florida. I ask this because, in addition to attempting to comply with the Federal CLIA regs governing labs, Florida has personnel licensure regs that cover our staff. So, can an ASCP-registered technician (HT, MLT) handle these procedures, or does it require a technologist (HTL, MT)? And what about unregistered/unlicensed people who work in physicians-office labs? I'm especially interested in receiving feedback from the board members of the FL Society for Histotechnology and respresentatives from the FL Department of Health's division on Clinical Lab Personnel. From Luis.Chiriboga <@t> nyumc.org Fri Jun 1 13:17:18 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Fri Jun 1 13:20:08 2012 Subject: [Histonet] Special Stain query Message-ID: <3E6798F00C9F494399E96B720ECD1429070620@MSGWCDCPMB22.nyumc.org> Does anyone have a method/protocol for Pinkus?acid orcein-giemsa stain? Thanks and good weekend to all Luis Chiriboga Experimental Pathology Core Laboratory New York University School of Medicine From rjbuesa <@t> yahoo.com Fri Jun 1 13:35:08 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 1 13:35:12 2012 Subject: [Histonet] Special Stain query In-Reply-To: <3E6798F00C9F494399E96B720ECD1429070620@MSGWCDCPMB22.nyumc.org> Message-ID: <1338575708.32030.YahooMailClassic@web162105.mail.bf1.yahoo.com> Reagents: Sol.A = water 30mL + 95%EthOL 70mL + HCl 0.6mL + orcein 1 g Sol.B = HCl 0.3mL + absolute EthOL 97mL Sol.C = Giemsa solution (0.05% eosin + 0.8% azur 5) 0.6mL + water 100mL Sol.D = 0.001% eosin Y in 95%EthOL ? Method: sections ? 70%EthOL ? A x ? to 1 hour ? rinse ? 95% EthOL a few dips ? 100% EthOL til decolorized to pale brwon ? B till almost completely decolorized ? wash ? C x 2 to 12 hours ? blot dry ? D till dehydrate ? clear ? mount ? Recommended for skin section. Ref.: Peter Gray pp 424 Method 21.412 Pinkus 1944 There you have it! Ren? J. --- On Fri, 6/1/12, Chiriboga, Luis wrote: From: Chiriboga, Luis Subject: [Histonet] Special Stain query To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, June 1, 2012, 2:17 PM Does anyone have a method/protocol for Pinkus?acid orcein-giemsa stain? Thanks and good weekend to all Luis Chiriboga Experimental Pathology Core Laboratory New York University School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Fri Jun 1 14:22:21 2012 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Jun 1 14:22:54 2012 Subject: [Histonet] Anatomic Pathology Coder/Biller Message-ID: <4FC8D01E.5D38.00EF.0@swmail.sw.org> I am presently writing a job description for the above position. Does anyone have such a person in their lab? Could you share with me their job description/salary range, etc. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From dholmes <@t> umc.edu Fri Jun 1 17:04:21 2012 From: dholmes <@t> umc.edu (Dianne E. Holmes) Date: Fri Jun 1 17:04:30 2012 Subject: [Histonet] Stains Message-ID: Does anyone know a retic stain that does NOT use uranyl nitrate? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From tahseen <@t> brain.net.pk Sat Jun 2 02:30:50 2012 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sat Jun 2 02:31:08 2012 Subject: [Histonet] DFA Method for the diagnosis of PCP on sputa. Message-ID: <43686.203.135.35.66.1338622250.squirrel@brain.net.pk> Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT (Japan) Senior Supervisor Histopathology SKMCH&RC Lahore Pakistan From jaylundgren <@t> gmail.com Sat Jun 2 10:07:39 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Sat Jun 2 10:07:45 2012 Subject: [Histonet] In FL, who can perform IHC/ISH? In-Reply-To: References: Message-ID: I had an assignment in SW Florida not too long ago, doing mostly IHC, and at that time I was told that FL requires a tech doing IHC to be a Florida registered Technologist. (Florida has three levels of registration, technician, technologist, and supervisor.) *NOTA BENE*: This is NOT the same thing as an HTL (ASCP). To find the requirements for a Florida registered Technologist, go the the FL DOH website. It's almost time to renew mine. :) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) : From rjbuesa <@t> yahoo.com Sat Jun 2 10:53:12 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jun 2 10:53:17 2012 Subject: [Histonet] DFA Method for the diagnosis of PCP on sputa. In-Reply-To: <43686.203.135.35.66.1338622250.squirrel@brain.net.pk> Message-ID: <1338652392.83685.YahooMailClassic@web162101.mail.bf1.yahoo.com> Use Grocott's methenamine silver method. It has become the "standard" procedure for P.carinii Ren? J. --- On Sat, 6/2/12, tahseen@brain.net.pk wrote: From: tahseen@brain.net.pk Subject: [Histonet] DFA Method for the diagnosis of PCP on sputa. To: histonet@lists.utsouthwestern.edu Date: Saturday, June 2, 2012, 3:30 AM Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT (Japan) Senior Supervisor Histopathology SKMCH&RC Lahore Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jun 2 10:54:43 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jun 2 10:54:51 2012 Subject: [Histonet] Stains In-Reply-To: Message-ID: <1338652483.3355.YahooMailClassic@web162105.mail.bf1.yahoo.com> Use modified Steiner with phosphotungstic acid instead of uranyl nitrate. Ren? J. --- On Fri, 6/1/12, Dianne E. Holmes wrote: From: Dianne E. Holmes Subject: [Histonet] Stains To: "histonet@lists.utsouthwestern.edu" Date: Friday, June 1, 2012, 6:04 PM Does anyone know a retic stain that does NOT use uranyl? nitrate? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Jun 2 12:11:40 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Jun 2 12:11:50 2012 Subject: [Histonet] In FL, who can perform IHC/ISH? In-Reply-To: References: Message-ID: This is correct. The only exclusion is a private office lab only needs to meet CLIA sub part M requirements. The science courses basically CLIA has been lenient on this but are now getting tuffer with the new healthcare regulations coming out. I don't want to make this a threat but everyone needs to be diligent About upping their game. It's Saturday and I really don't want to get blasted Read CLIA sub part M and go to DOH fl site like jay recommended Cheers Sent from my iPhone On Jun 2, 2012, at 11:07 AM, Jay Lundgren wrote: > I had an assignment in SW Florida not too long ago, doing mostly IHC, > and at that time I was told that FL requires a tech doing IHC to be a > Florida registered Technologist. (Florida has three levels of > registration, technician, technologist, and supervisor.) *NOTA BENE*: > This is NOT the same thing as an HTL (ASCP). To find the requirements for > a Florida registered Technologist, go the the FL DOH website. It's almost > time to renew mine. :) > > Sincerely, > > Jay A. Lundgren, M.S., > HTL (ASCP) > > > > > > > > > > > > : > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Jun 2 12:52:11 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Jun 2 12:52:18 2012 Subject: [Histonet] In FL, who can perform IHC/ISH? In-Reply-To: References: Message-ID: <874D74AB-448C-4741-A36F-4633F00CA827@yahoo.com> All sorry about my double posting. My iPhone must have an echo. :( Enjoy life! Sent from my iPhone On Jun 2, 2012, at 1:11 PM, Kim Donadio wrote: > This is correct. The only exclusion is a private office lab only needs to meet CLIA sub part M requirements. The science courses basically > CLIA has been lenient on this but are now getting tuffer with the new healthcare regulations coming out. > > I don't want to make this a threat but everyone needs to be diligent About upping their game. It's Saturday and I really don't want to get blasted > > Read CLIA sub part M and go to DOH fl site like jay recommended > > Cheers > > > Sent from my iPhone > > On Jun 2, 2012, at 11:07 AM, Jay Lundgren wrote: > >> I had an assignment in SW Florida not too long ago, doing mostly IHC, >> and at that time I was told that FL requires a tech doing IHC to be a >> Florida registered Technologist. (Florida has three levels of >> registration, technician, technologist, and supervisor.) *NOTA BENE*: >> This is NOT the same thing as an HTL (ASCP). To find the requirements for >> a Florida registered Technologist, go the the FL DOH website. It's almost >> time to renew mine. :) >> >> Sincerely, >> >> Jay A. Lundgren, M.S., >> HTL (ASCP) >> >> >> >> >> >> >> >> >> >> >> >> : >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Jun 2 13:10:49 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jun 2 13:10:53 2012 Subject: [Histonet] Pinkus' acid orcein Giemsa stain Message-ID: Luis Chiriboga (at NYU) asks for a method for Pinkus' acid orcein Giemsa stain. I haven't performed this stain or seen it done, but I copied the method out of Pinkus' book quite a few years ago. I don't know if anybody is still doing this stain. Orcein is a natural dye (from a species of lichen) which can be synthesized, though the synthetic dye is different from the natural one. Pinkus specified the synthetic and gave a source (the old Harleco). Bob Richmond Samurai Pathologist Knoxville TN ************************************************ Mehregan, Amir H. (Wayne State). Pinkus? Guide to Dermatohistopathology. 4th ed. Appleton-Century-Crofts, 1986. Hermann Pinkus, 1905-1985 Acid orcein and Giemsa stain: from Pinkus H, Hunter R. Simplified acid orcein and Giemsa technique for routine staining of skin sections. Arch Dermatol 82:699, 1960 Krobock E, Rahbari H, Mehregan AH. Acid orcein and Giemsa stain. Modification of a valuable stain for dermatologic specimens. J Cutan Pathol 5:37, 1978. Fix in formalin or alcohol, without chromium or mercury. 1. Stain in ORCEIN for 30 minutes. Synthetic orcein stains elastic fibers specifically, with very little background staining. The background may be decolorized by short immersion in absolute alcohol or 0.1% acid alcohol. 2. Wash in running water 10 minutes. 3. Stain overnight in GIEMSA. Krobock et al. speeded this up by staining 1 hour in 1% Giemsa solution at 60? C. 4. Wipe slides. Remove excess blue by rinsing in 95% alcohol to which a small amount of eosin has been added if necessary. Continue until the collagen of the skin looks pink. Then dehydrate. ORCEIN: dissolve 200 mg of Harleco?s synthetic Orcein in 100 mL of 70% alcohol. Add 0.6 mL of concentrated HCl. The solution improves on standing and has a long shelf life. GIEMSA: one drop of stock in 20 mL of distilled water or pH 7.0 phosphate buffer. RESULTS: Collagen is rose-pink, while elastic is dark brown to black. Melanin is dark green to black. Bacteria and fungi are dark blue. Looking at the book, I would suppose that Mehregan had just about abandoned the use of this stain. From amosbrooks <@t> gmail.com Sat Jun 2 13:31:20 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Jun 2 13:31:25 2012 Subject: [Histonet] BLock alignment tool Message-ID: Hi, There is one on every microtome made. The two little knobs align the block just perfectly to any angle previously cut. It is much better than just whacking into a block that was cut slightly differently than what a dumb instrument is telling you is aligned. Knowing how to use this alignment tool should be the first lesson. (Well ok maybe the second, the first being don't cut yourself!) This is like driving down the road with a clamp on your steering wheel complaining about the curve in the road causing you to hit a tree. Amos On Fri, Jun 1, 2012 at 10:32 AM, wrote: > Message: 2 > Date: Thu, 31 May 2012 13:48:26 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] BLock alignment tool > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com > > > Content-Type: text/plain; charset="us-ascii" > > Awhile back we got an advertisement for a block alignment tool that would > be helpful in recut cases. Can anyone share with me companies that have > this device and approximate costs? > > James Vickroy BS, HT(ASCP) > From rllott <@t> bellsouth.net Sat Jun 2 14:23:23 2012 From: rllott <@t> bellsouth.net (Robert L. Lott) Date: Sat Jun 2 14:23:37 2012 Subject: [Histonet] Retic Stains Message-ID: The Gordon and Sweets, Gomori, Laidlaw, and Nasher and Shanklin methods for reticulin do NOT use Uranyl nitrate as a sensitizer. The Snook and Wilder methods do. Robert L. Lott, HTL(ASCP) From: Dianne E. Holmes Subject: [Histonet] Stains To: "histonet@lists.utsouthwestern.edu" Date: Friday, June 1, 2012, 6:04 PM Does anyone know a retic stain that does NOT use uranyl nitrate? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From one_angel_secret <@t> yahoo.com Sat Jun 2 14:23:56 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Jun 2 14:24:02 2012 Subject: [Histonet] BLock alignment tool In-Reply-To: References: Message-ID: I thought a alignment tool was one of those weird things histotechs used to make sure their toenails were even on all 5 toes ? Dang! Sent from my iPhone On Jun 2, 2012, at 2:31 PM, Amos Brooks wrote: > Hi, > There is one on every microtome made. The two little knobs align the > block just perfectly to any angle previously cut. It is much better than > just whacking into a block that was cut slightly differently than what a > dumb instrument is telling you is aligned. Knowing how to use this > alignment tool should be the first lesson. (Well ok maybe the second, the > first being don't cut yourself!) This is like driving down the road with a > clamp on your steering wheel complaining about the curve in the road > causing you to hit a tree. > > Amos > > On Fri, Jun 1, 2012 at 10:32 AM, > wrote: > >> Message: 2 >> Date: Thu, 31 May 2012 13:48:26 -0500 >> From: "Vickroy, Jim" >> Subject: [Histonet] BLock alignment tool >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF5B0@mmc-mail.ad.mhsil.com >>> >> Content-Type: text/plain; charset="us-ascii" >> >> Awhile back we got an advertisement for a block alignment tool that would >> be helpful in recut cases. Can anyone share with me companies that have >> this device and approximate costs? >> >> James Vickroy BS, HT(ASCP) >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Jun 3 19:11:19 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Jun 3 19:11:26 2012 Subject: [Histonet] DIRECTIONS TO UNSUBSCRIBE In-Reply-To: References: <107F5E0D3BEF5843BF653A6F629694F624AE7A@mcpexchange.mcp.local> Message-ID: DIRECTIONS TO UNSUBSCRIBE - it's a do-it-yourself: - from the computer you are receiving the Histonet emails: - open any email from Histonet - scroll to the bottom of the email - click on the http link - scroll to the bottom of the new link, look for a statement about "To unsubscribe from Histonet" - put in your email in the blank - click on unsubscribe Peggy Wenk -----Original Message----- From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Thursday, May 31, 2012 11:41 AM To: WILLIAM DESALVO ; dtaylor@mcpathology.com ; histonet Subject: RE: [Histonet] please unsubscribe Too funny! Everyone, UNSUBSCRIBE YOURSELF! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, May 31, 2012 11:40 AM To: dtaylor@mcpathology.com; histonet Subject: RE: [Histonet] please unsubscribe #116 - WHEN WILL IT STOP????? William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 31 May 2012 11:34:52 -0400 > From: dtaylor@mcpathology.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] please unsubscribe > > Please unsubscribe me from this list. Thanks. > > > > Deborah Taylor, MS > > Customer Relations/Lab Manager > > Marlboro Chesterfield Pathology, PC > > 672 Hwy 9 West > > Bennettsville, SC 29512 > > Phone: 843-479-2402 > > Fax: 843-479-6609 > > > > > > Note: The information in this message is confidential and may be > legally privileged. It is intended solely for the addressee. Access to > this message by anone else is unauthorized. If you are not the > intended recipient, any disclosure copying or distribution of the > message or any action or omission taken by you in reliance on it, is > prohibitied and may be unlawful. Please immediately contact the sender > if you have received this message in error. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From minhan.science <@t> gmail.com Sun Jun 3 19:49:49 2012 From: minhan.science <@t> gmail.com (Min-Han Tan) Date: Sun Jun 3 19:49:52 2012 Subject: [Histonet] Formaldehyde concentration assay Message-ID: Dear Histonet members, I would like to evaluate our NBF and formaldehyde aliquots to ensure that it is at 4%, 2% etc. I would guess I need an assay between 0 - 5%? Does anyone know a quick chemical experiment to test this? I have access to all common lab chemicals. I can see that there are kits available e.g. http://www.hannacan.com/PDF/manHI3838.pdf but I really don't need so many tests; neither do I really need the ultrasensitive formaldehyde assays for urine. Your advice is appreciated - thanks! Regards, Min-Han From rnegron <@t> sarapath.com Sun Jun 3 20:44:40 2012 From: rnegron <@t> sarapath.com (Robin Negron) Date: Sun Jun 3 20:40:50 2012 Subject: [Histonet] Opinions for the On-Demand Cassette Engravers vs. Batch Engraver Message-ID: <329AC0C6085B0440B5B8EDF24FB002B503D8EB28@SPEX10.sarapath.com> I would like to know if anyone is currently using the on demand cassette engravers at the grossing table? If so can you share your Likes or dislikes and how this has helped or hindered your process. I can also use any feed back from others for batch printers if anyone has purchased a new one recently. Thanks, Robin Negron From JMaslanka <@t> stpetes.org Mon Jun 4 11:11:13 2012 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Mon Jun 4 11:11:36 2012 Subject: [Histonet] IHC Procedure-Napsin A Message-ID: Our IHC tech is having trouble validating NAPSIN A, we are running it on a benchmark XT. She's been working with tech support at Ventana to no avail. Any suggestions would be appreciated. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... From TJohnson <@t> gnf.org Mon Jun 4 12:15:28 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Jun 4 12:15:40 2012 Subject: [Histonet] Research Associate - Temporary position San Diego Message-ID: <9F3CFEE76E51B64991C7485270890B4009F5C0BC@EX5.lj.gnf.org> Dear Histonetters, We currently have an opening for a temporary Research Associate. Here is the direct link to the listing on the company website: http://www.gnf.org/careers/job_20120524.htm Job Code: TJ05-016 Description: GNF is currently seeking a highly motivated temporary scientific associate to join the Histology group.? Performs routine, special staining and complex procedures necessary for preparing specimens of animal tissue in a research environment. Qualifications: .Minimum Associate's degree in a biological science or equivalent experience is required. .American Society of Clinical Pathologist (ASCP) certification as a Histology Technician is preferred. .Applicants must demonstrate the ability to perform the essential functions of the job as outlined in the position description.? Experience: Applicant should have at least 2 years of experience in histologic techniques in a research environment, a wide variety of histochemical? staining, and fluorescent and chromogenic immunohistochemistry. Essential Functions: .Identifies significant tissue elements microscopically to determine quality of staining. .Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. .Performs microtomy on rotary microtome, cryostat. .Prepares dyes and solutions in order to perform special or complex procedures. .Has the background to conduct basic histochemical stains and enzymatic histochemical stains. Have the background in Immunohistochemical staining and other advanced histological procedures. .Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. .Operate Slide scanning instrumentation. .Ability to work as a team in a fast paced environment.? Send CV/resume as attachment to the email address listed below (please include job code in the subject line). Contact: E-mail: jobs@gnf.org Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From sadey <@t> hotmail.ca Mon Jun 4 13:19:07 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Mon Jun 4 13:19:14 2012 Subject: [Histonet] Slide distribution amongst Pathologists Message-ID: Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 From gu.lang <@t> gmx.at Mon Jun 4 13:33:34 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Jun 4 13:33:40 2012 Subject: AW: [Histonet] Slide distribution amongst Pathologists In-Reply-To: References: Message-ID: <003701cd4280$8cc1f160$a645d420$@gmx.at> Here the slides go through the hands of one pathologist, who distributes the cases. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey Gesendet: Montag, 04. Juni 2012 20:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Mon Jun 4 13:33:35 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Mon Jun 4 13:33:42 2012 Subject: [Histonet] Cytology Guru Message-ID: Happy Monday to everyone in Histoland, I am looking for a cytology guru, especially one that deals with processing and staining of non-gyn cytologies. If you are out there and don't mind me calling you please privately email me your contact information. We are having some processing and staining issues within our lab and I know that there has to be something easier out there than what we are doing, which is everything manually with non-gyn cytologies. Thanks in advance for your help, Amy Amy Self Georgetown Hospital System Georgetown, SC 843-527-7179 (p) NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From mpence <@t> grhs.net Mon Jun 4 13:39:30 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jun 4 13:39:34 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E39@is-e2k3.grhs.net> The only way this will be worked out that everyone feels it is fair is if the 5 Dr's set down and talk it out. That way everyone has their say and input. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Monday, June 04, 2012 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Jun 4 13:44:36 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Jun 4 13:44:49 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974E39@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974E39@is-e2k3.grhs.net> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27415ADF1B@NADCWPMSGCMS03.hca.corpad.net> We divide the work up by trays and mark the number of trays on a dry erase board every day. I have 3 1/2 (1 part time) Pathologist and each day one is Primary, one is secondary and then there is the Cytology/Frozen section Pathologist. They rotate this each day. When the Hemepath guy has a lot of BM slides, we give him less surgical trays. They are great about helping each other out if one is super busy!!! WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, June 04, 2012 2:40 PM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists The only way this will be worked out that everyone feels it is fair is if the 5 Dr's set down and talk it out. That way everyone has their say and input. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Monday, June 04, 2012 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Mon Jun 4 17:14:39 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jun 4 17:14:44 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974E39@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974E39@is-e2k3.grhs.net> Message-ID: <660E23CE-5E82-4676-A927-38C8C94ECC29@email.arizona.edu> Mike is right. You will never satisfy 5 pathologists by making the decision yourself. They have to collectively agree on how they want it done otherwise somebody will get bent out of shape because they think their portion on the work is greater than others and it is unfair. You could be at the meeting and make suggestions but they need to make the final decision. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From wilson6848 <@t> yahoo.com Mon Jun 4 22:46:59 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Mon Jun 4 22:47:02 2012 Subject: [Histonet] IHC Procedures Message-ID: <1338868019.63557.YahooMailNeo@web120903.mail.ne1.yahoo.com> ? ? ?Hi guys, ???????? We are preparing for CAP Inspection and we were suppose to write procedues/policieson stuffs like Buffer water, positive controls, negative controls.? ETC. ?? So please I will appreciate it if somebody can get these procedure out? to me. ?? I really appreciate you guys. ? ? ?? Thanks, ? ?Wilson. From Susan.Walzer <@t> HCAHealthcare.com Tue Jun 5 02:21:16 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Jun 5 02:21:37 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: <003701cd4280$8cc1f160$a645d420$@gmx.at> References: <003701cd4280$8cc1f160$a645d420$@gmx.at> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DEC33D7DF@FWDCWPMSGCMS09.hca.corpad.net> Boy, they sure like to put us in the middle of what should be their own problem. Thank heavens I only work now for one Dr at a time now but when I was at a larger place they rotated. They still used to tell us to give them particular cases when it was not their turn so that we got the flak when someone did not get what they thought was theirs. You can never win! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, June 04, 2012 2:34 PM To: 'Sheila Adey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Slide distribution amongst Pathologists Here the slides go through the hands of one pathologist, who distributes the cases. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey Gesendet: Montag, 04. Juni 2012 20:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Jun 5 05:01:52 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jun 5 05:02:04 2012 Subject: [Histonet] View Transit of Venus Message-ID: Now for something totally non-histology related ? For those of us in the basement, who hardly ever see the sun: Literally a once-in-a-lifetime (about every 112 years) event ? Venus will pass in front of our Sun tonight Tues June 5. Starting about 5:45 pm Eastern time, for about 3 hours. All of North American should be able to see it. (Those in other countries ? check the NASA website below.) Do NOT look at the sun directly. Do NOT look at it with regular sunglasses. Use welders glass, pin hole camera, or telescope with solar filter are OK. Demonstrations of how to do these: http://www.transitofvenus.org/june2012/eye-safety/281-six-ways-to-see-the-transit-of-venus Or watch on live NASA broadcast. http://sunearthday.nasa.gov/2012/transit/webcast.php Have fun! Peggy A. Wenk, HTL(ASCP)SLS From tpodawiltz <@t> lrgh.org Tue Jun 5 05:05:32 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Jun 5 05:05:44 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DEC33D7DF@FWDCWPMSGCMS09.hca.corpad.net> References: <003701cd4280$8cc1f160$a645d420$@gmx.at> <4BF03F5404EBDE409AF9232DA74B9DED2DEC33D7DF@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FBA8227A@LRGHEXVS1.practice.lrgh.org> We have one simple way of doing it. He who Grosses is he who reads. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Tuesday, June 05, 2012 3:21 AM To: gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists Boy, they sure like to put us in the middle of what should be their own problem. Thank heavens I only work now for one Dr at a time now but when I was at a larger place they rotated. They still used to tell us to give them particular cases when it was not their turn so that we got the flak when someone did not get what they thought was theirs. You can never win! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, June 04, 2012 2:34 PM To: 'Sheila Adey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Slide distribution amongst Pathologists Here the slides go through the hands of one pathologist, who distributes the cases. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey Gesendet: Montag, 04. Juni 2012 20:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From trathborne <@t> somerset-healthcare.com Tue Jun 5 07:40:34 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jun 5 07:39:35 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386324FBA8227A@LRGHEXVS1.practice.lrgh.org> References: <003701cd4280$8cc1f160$a645d420$@gmx.at> <4BF03F5404EBDE409AF9232DA74B9DED2DEC33D7DF@FWDCWPMSGCMS09.hca.corpad.net> <38667E7FB77ECD4E91BFAEB8D986386324FBA8227A@LRGHEXVS1.practice.lrgh.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FE2E81@SMCMAIL01.somerset-healthcare.com> We do the same. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, June 05, 2012 6:06 AM To: Susan.Walzer@HCAHealthcare.com; gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists We have one simple way of doing it. He who Grosses is he who reads. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Tuesday, June 05, 2012 3:21 AM To: gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists Boy, they sure like to put us in the middle of what should be their own problem. Thank heavens I only work now for one Dr at a time now but when I was at a larger place they rotated. They still used to tell us to give them particular cases when it was not their turn so that we got the flak when someone did not get what they thought was theirs. You can never win! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, June 04, 2012 2:34 PM To: 'Sheila Adey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Slide distribution amongst Pathologists Here the slides go through the hands of one pathologist, who distributes the cases. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey Gesendet: Montag, 04. Juni 2012 20:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From kelly_colpitts <@t> hotmail.com Tue Jun 5 08:25:51 2012 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Tue Jun 5 08:25:59 2012 Subject: [Histonet] HPV control slides Message-ID: Happy Tuesday, We are currently looking for HPV positive control slides for both HPV high risk and HPV low risk probes. Would anyone happen to have a half dozen or so of each that they could share? Please contact me at my email address if you do. Thanks, Kelly Colpitts Nationwide Childrens Hospital Columbus, OH From talulahgosh <@t> gmail.com Tue Jun 5 09:15:45 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 5 09:15:53 2012 Subject: OT: [Histonet] View Transit of Venus Message-ID: Also, the ISS will be taking close-up pictures of Venus, the first time the pictures will be very detailed as the cupola wasn't on the ISS before. http://www.flickr.com/photos/nasa_jsc_photo/7164346718/in/set-72157629649730820/ Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Tue, Jun 5, 2012 at 6:01 AM, Lee & Peggy Wenk wrote: > Now for something totally non-histology related ? For those of us in the > basement, who hardly ever see the sun: > > Literally a once-in-a-lifetime (about every 112 years) event ? Venus will > pass in front of our Sun tonight Tues June 5. Starting about 5:45 pm > Eastern time, for about 3 hours. All of North American should be able to > see it. (Those in other countries ? check the NASA website below.) > > Do NOT look at the sun directly. Do NOT look at it with regular > sunglasses. Use welders glass, pin hole camera, or telescope with solar > filter are OK. Demonstrations of how to do these: > > http://www.transitofvenus.org/june2012/eye-safety/281-six-ways-to-see-the-transit-of-venus > > Or watch on live NASA broadcast. > http://sunearthday.nasa.gov/2012/transit/webcast.php > > Have fun! > > Peggy A. Wenk, HTL(ASCP)SLS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Tue Jun 5 09:37:33 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jun 5 09:37:42 2012 Subject: [Histonet] Fluorescence-filters Message-ID: <009101cd4328$be8c48f0$3ba4dad0$@gmx.at> Hi! Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration? We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year. What do you think? Is it time to change them. I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. - and unfortunately unexperienced doctors on reading of this special probe. Hoping for responses Gudrun Lang From rjbuesa <@t> yahoo.com Tue Jun 5 10:04:17 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 5 10:04:24 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: <009101cd4328$be8c48f0$3ba4dad0$@gmx.at> References: <009101cd4328$be8c48f0$3ba4dad0$@gmx.at> Message-ID: <1338908657.5899.YahooMailNeo@web121405.mail.ne1.yahoo.com> Hi Gudrun: What you wrote is "news for me". Dichroic filters with specific lambda transmission of good quality can be used for years. The filters?I used were Ernst Leitz, from the early 1950's and the transmission intensity remained strong. We checked the life duration of the mercury lamp, but not of the filters. If you are getting weaker signals perhaps is another cause. Ren? J. ________________________________ From: Gudrun Lang To: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 5, 2012 10:37 AM Subject: [Histonet] Fluorescence-filters Hi! Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration? We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year. What do you think? Is it time to change them. I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. - and unfortunately unexperienced doctors on reading of this special probe. Hoping for responses Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Jun 5 11:03:09 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jun 5 11:03:16 2012 Subject: [Histonet] Fluorescence-filters -2 Message-ID: <000301cd4334$b43ed730$1cbc8590$@gmx.at> I forgot: the filters are now 10 years old. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Dienstag, 05. Juni 2012 16:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fluorescence-filters Hi! Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration? We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year. What do you think? Is it time to change them. I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. - and unfortunately unexperienced doctors on reading of this special probe. Hoping for responses Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> cbiolabs.com Tue Jun 5 11:39:42 2012 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Jun 5 11:39:58 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: <009101cd4328$be8c48f0$3ba4dad0$@gmx.at> References: <009101cd4328$be8c48f0$3ba4dad0$@gmx.at> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C9A53C8DF@cbiolabs05.CBiolabs.local> Filters are usually very stable. What is burning out are fiber optic cables that are present in some illumination systems such as X-Cite in Zeiss microscopes. These optical fiber cables should be replaced after 2000 hours. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, June 05, 2012 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fluorescence-filters Hi! Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration? We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year. What do you think? Is it time to change them. I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. - and unfortunately unexperienced doctors on reading of this special probe. Hoping for responses Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From perrintoshia <@t> yahoo.com Tue Jun 5 13:04:50 2012 From: perrintoshia <@t> yahoo.com (Mark Perrin) Date: Tue Jun 5 13:05:57 2012 Subject: [Histonet] Reagent rotation Message-ID: <1338919490.15637.YahooMailNeo@web44714.mail.sp1.yahoo.com> What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From TGoins <@t> mt.gov Tue Jun 5 13:35:14 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Jun 5 13:35:26 2012 Subject: [Histonet] Reagent rotation In-Reply-To: <1338919490.15637.YahooMailNeo@web44714.mail.sp1.yahoo.com> References: <1338919490.15637.YahooMailNeo@web44714.mail.sp1.yahoo.com> Message-ID: We rotate by cassette number - our daily load can vary from 10 to more than 100 cassettes, so an exchange schedule based on number of runs was not appropriate. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SStephenson <@t> lifecell.com Tue Jun 5 14:02:36 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Tue Jun 5 14:02:40 2012 Subject: [Histonet] Reagent rotation Message-ID: We do it by the number of Runs. About every 6 run for rotations and 12 runs for a full change out. But we also have another processor as well. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax 908.947.1085 Direct: 908.947.1624 ?sstephenson@lifecell.com 732. 939. 3037 Cell www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.Hoy <@t> UTSouthwestern.edu Tue Jun 5 15:30:19 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Tue Jun 5 15:30:29 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: <6daa0181c108412f8366874e414c85c7@SWMSHUB2.swmed.org> Message-ID: We do a LOT of fluorescent microscopy in our immunology lab, so I have a bit of experience with fluorescence. The answer to your question depends on what type of filters you have in your microscope, and what type of light source is on the microscope. Older fluorescent systems used absorption filters, which were simply discs of coloured glass. These filters had a fairly wide band-pass, so the fluorescence tended to be less than we see with interference filters. The good news with these filters is that they are nearly indestructible (unless you drop and break them.) Interference filters are produced by vacuum deposition of a thin film of metal vapour on high-quality glass. These filters usually have much sharper band pass characteristics than absorption filters. They are also considerably more expensive. If handled properly, these filters will last for decades, but improper cleaning and handling of the filters can shorten their lifespan. I have also heard that prolonged exposure to solvent vapours (such as we find in a histology lab), can damage the filters, although I have not seen any filters that suffered this type of damage. I have seen interference filters that show "delamination" of the metal film over time. In my experience these are older filters that were not produced with the current technologies, and filters that have been mishandled. Interference filters made in the past 20 years should last as long as the microscope, if they are properly handled. If you are seeing reduced fluorescence, I would suspect the light source as the most likely problem. Halogen lamps have less intensity than mercury vapour lamps, which are less intense than metal halide lamps, which are less intense than LED sources. We have converted all of our microscopes to LED sources. If you are using an older HBO or halogen lamp, the age of the lamp, the initial wattage of the lamp, and the alignment can all affect the fluorescent output. As you identified, perhaps the most important aspect of immunofluorescence is the skill and experience of the person who reads the slides. Let me know if you have further questions. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 6/5/12 9:37 AM, "Gudrun Lang" wrote: > Hi! > > Filters for fluorescencemicroscopy tend to "burn out" after a certain > duration of usage. What duration? > > We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours > are about 150 per year. > > > > What do you think? Is it time to change them. > > I have often bad feedback about weak signals, and I would not be surprised > if the microscope is the culprit and not our protocol. > > > > Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but > tumourcells mixed within collagenfibers. > > - and unfortunately unexperienced doctors on reading of this special probe. From brannon <@t> alliedsearchpartners.com Tue Jun 5 16:02:34 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Tue Jun 5 16:02:56 2012 Subject: [Histonet] Current Histology Openings Message-ID: Allied Search Partners is seeking qualified candidates for the following openings in Histology. 1) Night time Histology Supervisor- North of Los Angeles, CA 2) Histology Manager- Fort Myers, FL 3) Part time Mohs Tech- Denver, CO 4) IHC Technologist- Port Chester, NY Email/message me for full job descriptions! -- Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 From marktarango <@t> gmail.com Tue Jun 5 16:47:58 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jun 5 16:48:02 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: References: <6daa0181c108412f8366874e414c85c7@SWMSHUB2.swmed.org> Message-ID: Hi Gudrun, i read about 15 ALK cases a week. If you are seeing a lot of collagen fibers around the tumor cells, I'd try increasing the digestion time of your pepsin (especially if they were fixed for longer than usual). Before altering the pretreatment though, you would want to make sure that these slides were not exposed to light for any period of time. The discrete signals of the probes can quickly fade (much faster than with IF stained slides). I'd also make sure the door is closed in your dark room. If there is light in the corner of your eye the signals can be hard to see. ALK FISH should be scored at high power under oil immersion (60-100x objective). If they are scoring at 40x, it could be a problem. good luck! Mark Tarango On Tuesday, June 5, 2012, Eric Hoy wrote: > We do a LOT of fluorescent microscopy in our immunology lab, so I have a > bit > of experience with fluorescence. > > The answer to your question depends on what type of filters you have in > your > microscope, and what type of light source is on the microscope. > > Older fluorescent systems used absorption filters, which were simply discs > of coloured glass. These filters had a fairly wide band-pass, so the > fluorescence tended to be less than we see with interference filters. The > good news with these filters is that they are nearly indestructible (unless > you drop and break them.) > > Interference filters are produced by vacuum deposition of a thin film of > metal vapour on high-quality glass. These filters usually have much > sharper > band pass characteristics than absorption filters. They are also > considerably more expensive. If handled properly, these filters will last > for decades, but improper cleaning and handling of the filters can shorten > their lifespan. I have also heard that prolonged exposure to solvent > vapours (such as we find in a histology lab), can damage the filters, > although I have not seen any filters that suffered this type of damage. > > I have seen interference filters that show "delamination" of the metal film > over time. In my experience these are older filters that were not produced > with the current technologies, and filters that have been mishandled. > Interference filters made in the past 20 years should last as long as the > microscope, if they are properly handled. > > If you are seeing reduced fluorescence, I would suspect the light source as > the most likely problem. Halogen lamps have less intensity than mercury > vapour lamps, which are less intense than metal halide lamps, which are > less > intense than LED sources. We have converted all of our microscopes to LED > sources. If you are using an older HBO or halogen lamp, the age of the > lamp, the initial wattage of the lamp, and the alignment can all affect the > fluorescent output. > > As you identified, perhaps the most important aspect of immunofluorescence > is the skill and experience of the person who reads the slides. > > Let me know if you have further questions. > > Eric Hoy > > =============================================== > Eric S. Hoy, Ph.D., SI(ASCP) > Clinical Associate Professor > Department of Medical Laboratory Sciences > The University of Texas Southwestern Medical Center > Dallas, Texas > Email: Eric.Hoy@UTSouthwestern.edu > =============================================== > > On 6/5/12 9:37 AM, "Gudrun Lang" > wrote: > > > Hi! > > > > Filters for fluorescencemicroscopy tend to "burn out" after a certain > > duration of usage. What duration? > > > > We have filters for FITC, TRITC, Dapi and a triplefilter. The > working-hours > > are about 150 per year. > > > > > > > > What do you think? Is it time to change them. > > > > I have often bad feedback about weak signals, and I would not be > surprised > > if the microscope is the culprit and not our protocol. > > > > > > > > Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed > but > > tumourcells mixed within collagenfibers. > > > > - and unfortunately unexperienced doctors on reading of this special > probe. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mw <@t> personifysearch.com Tue Jun 5 17:27:32 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Jun 5 17:27:39 2012 Subject: [Histonet] NEW Position Alert - Quality Assurance Histologist Message-ID: <855477cea9188eab486251231404faa7@mail.gmail.com> Good Evening Histonet, We are currently searching for a Histotech that would be interested in a QA role with a global leader in Histology based in Richmond IL. The company is growing and offers a strong package. Please respond to this e-mail with your resume if you would be interested in learning more. *Quality Assurance Histologist * *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacturing and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunity:* The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. This position reports to the Quality Assurance Manager. All applicants must not be adverse to travel, as this is a position that may require domestic and international travel when necessary. Salary: Commensurate with experience Other: Full benefits - 401k program/matching *Primary Responsibilities: * The primary purpose for this position is to perform product specification and functionality testing on incoming raw material and final products to release for distribution. Additional Responsibilities: - Perform incoming, in process and final inspection on product for release - Effectively communicate the status of product testing - Assist in the maintenance of laboratory facilities, equipment and consumables - Perform additional testing and investigation related to customer complaints - Maintain laboratory equipment - Good documentation practices - Necessary computer skills, Microsoft Word, Excel, and Powerpoint. Use of SAP is preferable - A commitment to follow standard company financial procedures - Achieve best practice safety performance levels - Product training and quality systems training *Education and Experience Required:* - Ability to work independently and as part of a team - Able to perform tissue grossing, tissue processing, embedding - Sectioning paraffin embedded tissue as well as frozen tissue - Performing routine stains (H and E) as well as special stains - Formulation and production of routine laboratory reagents and solutions - Performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Good communication skills both verbal and written - Proficiency in basic computer skills and with software applications such as Microsoft Office Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From talulahgosh <@t> gmail.com Tue Jun 5 18:27:34 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 5 18:27:38 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: References: <6daa0181c108412f8366874e414c85c7@SWMSHUB2.swmed.org> Message-ID: Wow, that was detailed and interesting!! I didn't know microscopes could even use LED, does that require a different setup, or just a different bulb? Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh From talulahgosh <@t> gmail.com Tue Jun 5 18:31:39 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 5 18:31:43 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: References: <6daa0181c108412f8366874e414c85c7@SWMSHUB2.swmed.org> Message-ID: Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh From ibernard <@t> uab.edu Tue Jun 5 20:22:40 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Tue Jun 5 20:22:48 2012 Subject: [Histonet] Reagent rotation In-Reply-To: References: Message-ID: We do it by number of blocks- At or after 350 blocks the processor is changed. Ian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Tuesday, June 05, 2012 2:03 PM To: 'Mark Perrin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation We do it by the number of Runs. About every 6 run for rotations and 12 runs for a full change out. But we also have another processor as well. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax 908.947.1085 Direct: 908.947.1624 ?sstephenson@lifecell.com 732. 939. 3037 Cell www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Cremer <@t> med.kuleuven.be Wed Jun 6 01:18:00 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Jun 6 01:18:13 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: References: <6daa0181c108412f8366874e414c85c7@SWMSHUB2.swmed.org> , Message-ID: Sure you can, all you need is a bright enough lightsource which emits at the correct wavelength. If the spectrum of the light source is too wide, you dump in a filter to narrow it down to your desired excitation. Flow cytometers nowadays even use LED lasers instead of the huge, bulky and vulnerable gas lasers of the old days. Which is a good thing. Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Emily Sours [talulahgosh@gmail.com] Verzonden: woensdag 6 juni 2012 1:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Wed Jun 6 06:39:54 2012 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Jun 6 06:40:03 2012 Subject: [Histonet] Fluorescence-filters In-Reply-To: References: <6daa0181c108412f8366874e414c85c7@SWMSHUB2.swmed.org> Message-ID: We recently installed a LED light source on our fluorescence scope that our pathologists use for reading IF on kidneys and skins. They love it. No more having to warm up the bulb, not turning it off too soon, or my nightmare - leaving it on overnight. The bulb is supposed to last a really long time and gives off a nice bright light. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, June 05, 2012 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet To report this email as SPAM, please forward it to spam@websense.com. From ADuddey <@t> firsthealth.org Wed Jun 6 07:30:47 2012 From: ADuddey <@t> firsthealth.org (Duddey, Aimee) Date: Wed Jun 6 07:31:03 2012 Subject: [Histonet] Histotech scheduling Message-ID: I am looking for some creative scheduling ideas. We have a day shift crew that starts at 4am and goes til 530pm. The schedule is below. Techs would like to rotate to do different jobs within the department but don't really want to rotate hours. Can I please get some examples of how others of you are staffing? Even if you have a second or third shift I would like to see some different workflow plans and how they are staffed. 1 HT 400am - Embedder 2 HTs 500am - Cutters 1 HT 730 - Immunos/specials/orders 1 HT 830 - Grossing/pathologist assist 1 lab assistant 800am - cytology prep/gross room assist Aimee M. Duddey, MLT(ASCP) Moore Regional Hospital Laboratory Assistant Director of Laboratory - Pathology 910-715-5286 FirstHealth OF THE CAROLINAS From joelleweaver <@t> hotmail.com Wed Jun 6 09:14:05 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jun 6 09:14:14 2012 Subject: [Histonet] Histotech scheduling In-Reply-To: References: Message-ID: http://www.biomedcentral.com/1472-6890/10/2 Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 6 Jun 2012 08:30:47 -0400 > From: ADuddey@firsthealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histotech scheduling > > I am looking for some creative scheduling ideas. We have a day shift > crew that starts at 4am and goes til 530pm. The schedule is below. > Techs would like to rotate to do different jobs within the department > but don't really want to rotate hours. Can I please get some examples > of how others of you are staffing? Even if you have a second or third > shift I would like to see some different workflow plans and how they are > staffed. > > > > 1 HT 400am - Embedder > > 2 HTs 500am - Cutters > > 1 HT 730 - Immunos/specials/orders > > 1 HT 830 - Grossing/pathologist assist > > 1 lab assistant 800am - cytology prep/gross room assist > > > > Aimee M. Duddey, MLT(ASCP) > > Moore Regional Hospital Laboratory > > Assistant Director of Laboratory - Pathology > > 910-715-5286 > > FirstHealth OF THE CAROLINAS > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Claire.Fahy <@t> agriculture.gov.ie Wed Jun 6 09:19:46 2012 From: Claire.Fahy <@t> agriculture.gov.ie (Fahy, Claire) Date: Wed Jun 6 09:19:58 2012 Subject: [Histonet] Histotech scheduling In-Reply-To: References: Message-ID: Please unsubscribe me. thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: 06 June 2012 15:14 To: aduddey@firsthealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotech scheduling http://www.biomedcentral.com/1472-6890/10/2 Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 6 Jun 2012 08:30:47 -0400 > From: ADuddey@firsthealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histotech scheduling > > I am looking for some creative scheduling ideas. We have a day shift > crew that starts at 4am and goes til 530pm. The schedule is below. > Techs would like to rotate to do different jobs within the department > but don't really want to rotate hours. Can I please get some examples > of how others of you are staffing? Even if you have a second or third > shift I would like to see some different workflow plans and how they > are staffed. > > > > 1 HT 400am - Embedder > > 2 HTs 500am - Cutters > > 1 HT 730 - Immunos/specials/orders > > 1 HT 830 - Grossing/pathologist assist > > 1 lab assistant 800am - cytology prep/gross room assist > > > > Aimee M. Duddey, MLT(ASCP) > > Moore Regional Hospital Laboratory > > Assistant Director of Laboratory - Pathology > > 910-715-5286 > > FirstHealth OF THE CAROLINAS > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Department of Agriculture, Food and the Marine The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha?ochta, Bia agus Mara T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. From AHutton <@t> dh.org Wed Jun 6 10:09:06 2012 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Jun 6 10:11:48 2012 Subject: [Histonet] staffing questions Message-ID: <38A56C4F4630D348A50B3720409270870E0FE5D6@dhmail.dhorg.org> I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs. If anybody is willing to answer the questions listed below, I would appreciate your input. You may email me at privately and all information will be kept confidental. 1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides 2. Approximate workload, either blocks per day or cases per year 3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful) Thank You in advance Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital ahutton@dh.org From rjbuesa <@t> yahoo.com Wed Jun 6 10:33:57 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 6 10:34:06 2012 Subject: [Histonet] staffing questions In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE5D6@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE5D6@dhmail.dhorg.org> Message-ID: <1338996837.66141.YahooMailNeo@web121402.mail.ne1.yahoo.com> Staff level (your question 1) depends on the answer to your question 2 and also depends on the productivity level, both individual and collective. The salaries depend on the nature of your lab (non for profit, private for profit or governmental) and with the total benefits offered. But also depend on the market availability and the willingness to negotiate both from the administration and the potential or existing employees. So you ca understand both issues I am sending you some information under separate cover. Ren? J. ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 6, 2012 11:09 AM Subject: [Histonet] staffing questions I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs.? If anybody is willing to answer the questions listed below, I would appreciate your input.? You may email me at privately and all information will be kept confidental. 1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides 2. Approximate workload, either blocks per day or cases per year 3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful) Thank You in advance Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital ahutton@dh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Keri.Colwell <@t> inspection.gc.ca Wed Jun 6 12:23:29 2012 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Wed Jun 6 12:23:37 2012 Subject: [Histonet] Sequenza units for manual immunohistochemistry References: <4FCF39DD020000BF00008D26@inspection.gc.ca> <4FCF3DB1020000BF00008D2F@inspection.gc.ca> Message-ID: <4FCF3DB1.C0AD.00BF.0@inspection.gc.ca> Hello Histonet, I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent "waste" in the bottom from all your additions and washes. Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom. Has anyone had any issues using these units? How do people prevent a large volume from building up in the bottom? How are people cleaning their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 ext 5613/5511 Facsimile | T?l?copieur: 403-382-5585 Government of Canada | Gouvernement du Canada From SLB <@t> stowers.org Wed Jun 6 13:23:41 2012 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Wed Jun 6 13:23:46 2012 Subject: [Histonet] Sequenza units for manual immunohistochemistry In-Reply-To: <4FCF3DB1.C0AD.00BF.0@inspection.gc.ca> References: <4FCF39DD020000BF00008D26@inspection.gc.ca> <4FCF3DB1020000BF00008D2F@inspection.gc.ca> <4FCF3DB1.C0AD.00BF.0@inspection.gc.ca> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9A0B65B11@EXCHMB-02.stowers-institute.org> I have used them for quite some time. I was having problems with uneven staining and determined that it was from the being too full and was drawing up liquid from the bottom. There is a small opening on the end and I just empty the solution from it. I usually empty it after rinsing the antibody off and then usually before putting DAB or DAPI on depending on the number of slides stained. As for washing, I just wash in a solution of soapy water and then rinse in distilled. I use the coverplates for several staining times. I love the sequenza and prefer it to having an automatic stainer. Sharon Beckham, HT (ASCP) Head, Histotechnology Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 (816)926-4305 slb@stowers.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Wednesday, June 06, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sequenza units for manual immunohistochemistry Hello Histonet, I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent "waste" in the bottom from all your additions and washes. Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom. Has anyone had any issues using these units? How do people prevent a large volume from building up in the bottom? How are people cleaning their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 ext 5613/5511 Facsimile | T?l?copieur: 403-382-5585 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM <@t> mcclainlab.com Wed Jun 6 15:32:32 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Wed Jun 6 15:33:12 2012 Subject: [Histonet] processor solution rotation Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF11B2FDC6@ML1.McClainLabs.local> May I suggest? Define a standard that works for you, combining both elements, blocks and runs e.g., Every week or 200 blocks then check the first reagent volume and quality. Again at 400 And again at 600 blocks. We change the first reagent whenever it appears dirty. Consider adding a few ccs. of eosin as a visual indicator in the first 100% alcohol to monitor carryover and number of runs. When the indicator reaches the first xylene, time to rotate or change. NOTE Eosin fluoresces. The actual blocks and runs number varies on specimen size and fixation, (unfixed tissues use up the alcohols) and also whether you use sponges (more carryover) and whether the processor drains/pumps out completely-carryover. And on how fast the processing cycle is- short cycles demand fresh reagents and leave little room for error. When in doubt do not hesitate to replenish the last solution of each type, alcohol, xylene and paraffin or change all solutions. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From melissa <@t> alliedsearchpartners.com Wed Jun 6 16:22:17 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed Jun 6 16:22:30 2012 Subject: [Histonet] Pathologists' Assistant Job in Mid-West Message-ID: Hi Everyone, I have a Pathologists' Assistant Job in the MidWest that is a full time permanent job. I am looking for an ASCP Certified Pathologists' Assistant, with a Master's Degree from an NAACLS accredited institution. Please email me for more details. Thank you@ To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From JASproles <@t> tmhs.org Wed Jun 6 16:29:57 2012 From: JASproles <@t> tmhs.org (Sproles, Judy A.) Date: Wed Jun 6 16:30:04 2012 Subject: [Histonet] Approval and review of procedures Message-ID: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 6 16:40:03 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jun 6 16:39:51 2012 Subject: [Histonet] RE: Approval and review of procedures In-Reply-To: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> References: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> Message-ID: <8D7C2D242DBD45498006B21122072BF8B54D4182@MCINFRWEM003.ucsfmedicalcenter.org> No, it has to be the pathologist who is designated as the technical supervisor for the lab. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. Sent: Wednesday, June 06, 2012 2:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Approval and review of procedures Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Jun 6 16:49:43 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Jun 6 16:49:51 2012 Subject: [Histonet] RE: Approval and review of procedures In-Reply-To: <8D7C2D242DBD45498006B21122072BF8B54D4182@MCINFRWEM003.ucsfmedicalcenter.org> References: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> <8D7C2D242DBD45498006B21122072BF8B54D4182@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: And not her/his designee? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, June 06, 2012 5:40 PM To: Sproles, Judy A.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Approval and review of procedures No, it has to be the pathologist who is designated as the technical supervisor for the lab. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. Sent: Wednesday, June 06, 2012 2:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Approval and review of procedures Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From vijaykanthg <@t> orchidpharma.com Wed Jun 6 23:20:36 2012 From: vijaykanthg <@t> orchidpharma.com (vijaykanthg@orchidpharma.com) Date: Wed Jun 6 23:20:53 2012 Subject: [Histonet] RE: Reagent rotation In-Reply-To: <96fef194-2dc6-4223-9908-191d49d0a562@CH1EHSMHS011.ehs.local> References: <96fef194-2dc6-4223-9908-191d49d0a562@CH1EHSMHS011.ehs.local> Message-ID: Dear Mark We change the solution depend upon no of cassettes run. In your case the size of specimen is small so u can change/ rotate depend upon no of run. i felt that the size of the specimen make big impact on reagents in Tissue Processer. With regards Dr G vijay Orchid Pharma -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, June 06, 2012 10:30 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 103, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Reagent rotation (Mark Perrin) 2. RE: Reagent rotation (Goins, Tresa) 3. Reagent rotation (Stephenson, Sheryl) 4. Re: Fluorescence-filters (Eric Hoy) 5. Current Histology Openings (Brannon Owens) 6. Re: Fluorescence-filters (Mark Tarango) 7. NEW Position Alert - Quality Assurance Histologist (Matt Ward) 8. Re: Fluorescence-filters (Emily Sours) 9. Re: Fluorescence-filters (Emily Sours) 10. RE: Reagent rotation (Ian R Bernard) 11. RE: Fluorescence-filters (Jonathan Cremer) 12. RE: Fluorescence-filters (Sue Hunter) 13. Histotech scheduling (Duddey, Aimee) 14. RE: Histotech scheduling (joelle weaver) 15. RE: Histotech scheduling (Fahy, Claire) 16. staffing questions (Hutton, Allison) 17. Re: staffing questions (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 5 Jun 2012 11:04:50 -0700 (PDT) From: Mark Perrin Subject: [Histonet] Reagent rotation To: "histonet@lists.utsouthwestern.edu" Message-ID: <1338919490.15637.YahooMailNeo@web44714.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. ------------------------------ Message: 2 Date: Tue, 5 Jun 2012 18:35:14 +0000 From: "Goins, Tresa" Subject: RE: [Histonet] Reagent rotation To: 'Mark Perrin' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We rotate by cassette number - our daily load can vary from 10 to more than 100 cassettes, so an exchange schedule based on number of runs was not appropriate. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 5 Jun 2012 14:02:36 -0500 From: "Stephenson, Sheryl" Subject: [Histonet] Reagent rotation To: 'Mark Perrin' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We do it by the number of Runs. About every 6 run for rotations and 12 runs for a full change out. But we also have another processor as well. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax 908.947.1085 Direct: 908.947.1624 ?sstephenson@lifecell.com 732. 939. 3037 Cell www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 05 Jun 2012 15:30:19 -0500 From: Eric Hoy Subject: Re: [Histonet] Fluorescence-filters To: Histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" We do a LOT of fluorescent microscopy in our immunology lab, so I have a bit of experience with fluorescence. The answer to your question depends on what type of filters you have in your microscope, and what type of light source is on the microscope. Older fluorescent systems used absorption filters, which were simply discs of coloured glass. These filters had a fairly wide band-pass, so the fluorescence tended to be less than we see with interference filters. The good news with these filters is that they are nearly indestructible (unless you drop and break them.) Interference filters are produced by vacuum deposition of a thin film of metal vapour on high-quality glass. These filters usually have much sharper band pass characteristics than absorption filters. They are also considerably more expensive. If handled properly, these filters will last for decades, but improper cleaning and handling of the filters can shorten their lifespan. I have also heard that prolonged exposure to solvent vapours (such as we find in a histology lab), can damage the filters, although I have not seen any filters that suffered this type of damage. I have seen interference filters that show "delamination" of the metal film over time. In my experience these are older filters that were not produced with the current technologies, and filters that have been mishandled. Interference filters made in the past 20 years should last as long as the microscope, if they are properly handled. If you are seeing reduced fluorescence, I would suspect the light source as the most likely problem. Halogen lamps have less intensity than mercury vapour lamps, which are less intense than metal halide lamps, which are less intense than LED sources. We have converted all of our microscopes to LED sources. If you are using an older HBO or halogen lamp, the age of the lamp, the initial wattage of the lamp, and the alignment can all affect the fluorescent output. As you identified, perhaps the most important aspect of immunofluorescence is the skill and experience of the person who reads the slides. Let me know if you have further questions. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 6/5/12 9:37 AM, "Gudrun Lang" wrote: > Hi! > > Filters for fluorescencemicroscopy tend to "burn out" after a certain > duration of usage. What duration? > > We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours > are about 150 per year. > > > > What do you think? Is it time to change them. > > I have often bad feedback about weak signals, and I would not be surprised > if the microscope is the culprit and not our protocol. > > > > Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but > tumourcells mixed within collagenfibers. > > - and unfortunately unexperienced doctors on reading of this special probe. ------------------------------ Message: 5 Date: Tue, 05 Jun 2012 17:02:34 -0400 From: Brannon Owens Subject: [Histonet] Current Histology Openings To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="US-ASCII" Allied Search Partners is seeking qualified candidates for the following openings in Histology. 1) Night time Histology Supervisor- North of Los Angeles, CA 2) Histology Manager- Fort Myers, FL 3) Part time Mohs Tech- Denver, CO 4) IHC Technologist- Port Chester, NY Email/message me for full job descriptions! -- Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 ------------------------------ Message: 6 Date: Tue, 5 Jun 2012 14:47:58 -0700 From: Mark Tarango Subject: Re: [Histonet] Fluorescence-filters To: Eric Hoy Cc: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Gudrun, i read about 15 ALK cases a week. If you are seeing a lot of collagen fibers around the tumor cells, I'd try increasing the digestion time of your pepsin (especially if they were fixed for longer than usual). Before altering the pretreatment though, you would want to make sure that these slides were not exposed to light for any period of time. The discrete signals of the probes can quickly fade (much faster than with IF stained slides). I'd also make sure the door is closed in your dark room. If there is light in the corner of your eye the signals can be hard to see. ALK FISH should be scored at high power under oil immersion (60-100x objective). If they are scoring at 40x, it could be a problem. good luck! Mark Tarango On Tuesday, June 5, 2012, Eric Hoy wrote: > We do a LOT of fluorescent microscopy in our immunology lab, so I have a > bit > of experience with fluorescence. > > The answer to your question depends on what type of filters you have in > your > microscope, and what type of light source is on the microscope. > > Older fluorescent systems used absorption filters, which were simply discs > of coloured glass. These filters had a fairly wide band-pass, so the > fluorescence tended to be less than we see with interference filters. The > good news with these filters is that they are nearly indestructible (unless > you drop and break them.) > > Interference filters are produced by vacuum deposition of a thin film of > metal vapour on high-quality glass. These filters usually have much > sharper > band pass characteristics than absorption filters. They are also > considerably more expensive. If handled properly, these filters will last > for decades, but improper cleaning and handling of the filters can shorten > their lifespan. I have also heard that prolonged exposure to solvent > vapours (such as we find in a histology lab), can damage the filters, > although I have not seen any filters that suffered this type of damage. > > I have seen interference filters that show "delamination" of the metal film > over time. In my experience these are older filters that were not produced > with the current technologies, and filters that have been mishandled. > Interference filters made in the past 20 years should last as long as the > microscope, if they are properly handled. > > If you are seeing reduced fluorescence, I would suspect the light source as > the most likely problem. Halogen lamps have less intensity than mercury > vapour lamps, which are less intense than metal halide lamps, which are > less > intense than LED sources. We have converted all of our microscopes to LED > sources. If you are using an older HBO or halogen lamp, the age of the > lamp, the initial wattage of the lamp, and the alignment can all affect the > fluorescent output. > > As you identified, perhaps the most important aspect of immunofluorescence > is the skill and experience of the person who reads the slides. > > Let me know if you have further questions. > > Eric Hoy > > =============================================== > Eric S. Hoy, Ph.D., SI(ASCP) > Clinical Associate Professor > Department of Medical Laboratory Sciences > The University of Texas Southwestern Medical Center > Dallas, Texas > Email: Eric.Hoy@UTSouthwestern.edu > =============================================== > > On 6/5/12 9:37 AM, "Gudrun Lang" > wrote: > > > Hi! > > > > Filters for fluorescencemicroscopy tend to "burn out" after a certain > > duration of usage. What duration? > > > > We have filters for FITC, TRITC, Dapi and a triplefilter. The > working-hours > > are about 150 per year. > > > > > > > > What do you think? Is it time to change them. > > > > I have often bad feedback about weak signals, and I would not be > surprised > > if the microscope is the culprit and not our protocol. > > > > > > > > Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed > but > > tumourcells mixed within collagenfibers. > > > > - and unfortunately unexperienced doctors on reading of this special > probe. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Tue, 5 Jun 2012 18:27:32 -0400 From: Matt Ward Subject: [Histonet] NEW Position Alert - Quality Assurance Histologist To: histonet@lists.utsouthwestern.edu Message-ID: <855477cea9188eab486251231404faa7@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Good Evening Histonet, We are currently searching for a Histotech that would be interested in a QA role with a global leader in Histology based in Richmond IL. The company is growing and offers a strong package. Please respond to this e-mail with your resume if you would be interested in learning more. *Quality Assurance Histologist * *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacturing and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunity:* The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. This position reports to the Quality Assurance Manager. All applicants must not be adverse to travel, as this is a position that may require domestic and international travel when necessary. Salary: Commensurate with experience Other: Full benefits - 401k program/matching *Primary Responsibilities: * The primary purpose for this position is to perform product specification and functionality testing on incoming raw material and final products to release for distribution. Additional Responsibilities: - Perform incoming, in process and final inspection on product for release - Effectively communicate the status of product testing - Assist in the maintenance of laboratory facilities, equipment and consumables - Perform additional testing and investigation related to customer complaints - Maintain laboratory equipment - Good documentation practices - Necessary computer skills, Microsoft Word, Excel, and Powerpoint. Use of SAP is preferable - A commitment to follow standard company financial procedures - Achieve best practice safety performance levels - Product training and quality systems training *Education and Experience Required:* - Ability to work independently and as part of a team - Able to perform tissue grossing, tissue processing, embedding - Sectioning paraffin embedded tissue as well as frozen tissue - Performing routine stains (H and E) as well as special stains - Formulation and production of routine laboratory reagents and solutions - Performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Good communication skills both verbal and written - Proficiency in basic computer skills and with software applications such as Microsoft Office Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ------------------------------ Message: 8 Date: Tue, 5 Jun 2012 19:27:34 -0400 From: Emily Sours Subject: Re: [Histonet] Fluorescence-filters To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Wow, that was detailed and interesting!! I didn't know microscopes could even use LED, does that require a different setup, or just a different bulb? Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh ------------------------------ Message: 9 Date: Tue, 5 Jun 2012 19:31:39 -0400 From: Emily Sours Subject: Re: [Histonet] Fluorescence-filters To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh ------------------------------ Message: 10 Date: Wed, 6 Jun 2012 01:22:40 +0000 From: Ian R Bernard Subject: RE: [Histonet] Reagent rotation To: "Stephenson, Sheryl" , 'Mark Perrin' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We do it by number of blocks- At or after 350 blocks the processor is changed. Ian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Tuesday, June 05, 2012 2:03 PM To: 'Mark Perrin'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation We do it by the number of Runs. About every 6 run for rotations and 12 runs for a full change out. But we also have another processor as well. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax 908.947.1085 Direct: 908.947.1624 ?sstephenson@lifecell.com 732. 939. 3037 Cell www.lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 6 Jun 2012 06:18:00 +0000 From: Jonathan Cremer Subject: RE: [Histonet] Fluorescence-filters To: Emily Sours , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Sure you can, all you need is a bright enough lightsource which emits at the correct wavelength. If the spectrum of the light source is too wide, you dump in a filter to narrow it down to your desired excitation. Flow cytometers nowadays even use LED lasers instead of the huge, bulky and vulnerable gas lasers of the old days. Which is a good thing. Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Emily Sours [talulahgosh@gmail.com] Verzonden: woensdag 6 juni 2012 1:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 6 Jun 2012 11:39:54 +0000 From: Sue Hunter Subject: RE: [Histonet] Fluorescence-filters To: Emily Sours , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" We recently installed a LED light source on our fluorescence scope that our pathologists use for reading IF on kidneys and skins. They love it. No more having to warm up the bulb, not turning it off too soon, or my nightmare - leaving it on overnight. The bulb is supposed to last a really long time and gives off a nice bright light. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, June 05, 2012 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet To report this email as SPAM, please forward it to spam@websense.com. ------------------------------ Message: 13 Date: Wed, 6 Jun 2012 08:30:47 -0400 From: "Duddey, Aimee" Subject: [Histonet] Histotech scheduling To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for some creative scheduling ideas. We have a day shift crew that starts at 4am and goes til 530pm. The schedule is below. Techs would like to rotate to do different jobs within the department but don't really want to rotate hours. Can I please get some examples of how others of you are staffing? Even if you have a second or third shift I would like to see some different workflow plans and how they are staffed. 1 HT 400am - Embedder 2 HTs 500am - Cutters 1 HT 730 - Immunos/specials/orders 1 HT 830 - Grossing/pathologist assist 1 lab assistant 800am - cytology prep/gross room assist Aimee M. Duddey, MLT(ASCP) Moore Regional Hospital Laboratory Assistant Director of Laboratory - Pathology 910-715-5286 FirstHealth OF THE CAROLINAS ------------------------------ Message: 14 Date: Wed, 6 Jun 2012 14:14:05 +0000 From: joelle weaver Subject: RE: [Histonet] Histotech scheduling To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" http://www.biomedcentral.com/1472-6890/10/2 Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 6 Jun 2012 08:30:47 -0400 > From: ADuddey@firsthealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histotech scheduling > > I am looking for some creative scheduling ideas. We have a day shift > crew that starts at 4am and goes til 530pm. The schedule is below. > Techs would like to rotate to do different jobs within the department > but don't really want to rotate hours. Can I please get some examples > of how others of you are staffing? Even if you have a second or third > shift I would like to see some different workflow plans and how they are > staffed. > > > > 1 HT 400am - Embedder > > 2 HTs 500am - Cutters > > 1 HT 730 - Immunos/specials/orders > > 1 HT 830 - Grossing/pathologist assist > > 1 lab assistant 800am - cytology prep/gross room assist > > > > Aimee M. Duddey, MLT(ASCP) > > Moore Regional Hospital Laboratory > > Assistant Director of Laboratory - Pathology > > 910-715-5286 > > FirstHealth OF THE CAROLINAS > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 6 Jun 2012 15:19:46 +0100 From: "Fahy, Claire" Subject: RE: [Histonet] Histotech scheduling To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Please unsubscribe me. thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: 06 June 2012 15:14 To: aduddey@firsthealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotech scheduling http://www.biomedcentral.com/1472-6890/10/2 Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 6 Jun 2012 08:30:47 -0400 > From: ADuddey@firsthealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histotech scheduling > > I am looking for some creative scheduling ideas. We have a day shift > crew that starts at 4am and goes til 530pm. The schedule is below. > Techs would like to rotate to do different jobs within the department > but don't really want to rotate hours. Can I please get some examples > of how others of you are staffing? Even if you have a second or third > shift I would like to see some different workflow plans and how they > are staffed. > > > > 1 HT 400am - Embedder > > 2 HTs 500am - Cutters > > 1 HT 730 - Immunos/specials/orders > > 1 HT 830 - Grossing/pathologist assist > > 1 lab assistant 800am - cytology prep/gross room assist > > > > Aimee M. Duddey, MLT(ASCP) > > Moore Regional Hospital Laboratory > > Assistant Director of Laboratory - Pathology > > 910-715-5286 > > FirstHealth OF THE CAROLINAS > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Department of Agriculture, Food and the Marine The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha??ochta, Bia agus Mara T?? an t-eolais san r??omhphost seo, agus in aon ceangl??in leis, faoi phribhl??id agus faoi r??n agus le h-aghaigh an seola?? amh??in. D???fh??adfadh ??bhar an seoladh seo bheith faoi phribhl??id profisi??nta n?? dl??thi??il. Mura tusa an seola?? a bh?? beartaithe leis an r??omhphost seo a fh??il, t?? cosc air, n?? aon chuid de, a ??s??id, a ch??ipe??l, n?? a scaoileadh. M?? th??inig s?? chugat de bharr dearmad, t??igh i dteagmh??il leis an seolt??ir agus scrios an t-??bhar ?? do r??omhaire le do thoil. ------------------------------ Message: 16 Date: Wed, 6 Jun 2012 11:09:06 -0400 From: "Hutton, Allison" Subject: [Histonet] staffing questions To: Message-ID: <38A56C4F4630D348A50B3720409270870E0FE5D6@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs. If anybody is willing to answer the questions listed below, I would appreciate your input. You may email me at privately and all information will be kept confidental. 1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides 2. Approximate workload, either blocks per day or cases per year 3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful) Thank You in advance Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital ahutton@dh.org ------------------------------ Message: 17 Date: Wed, 6 Jun 2012 08:33:57 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] staffing questions To: "Hutton, Allison" , "histonet@lists.utsouthwestern.edu" Message-ID: <1338996837.66141.YahooMailNeo@web121402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Staff level (your question 1) depends on the answer to your question 2 and also depends on the productivity level, both individual and collective. The salaries depend on the nature of your lab (non for profit, private for profit or governmental) and with the total benefits offered. But also depend on the market availability and the willingness to negotiate both from the administration and the potential or existing employees. So you ca understand both issues I am sending you some information under separate cover. Ren? J. ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 6, 2012 11:09 AM Subject: [Histonet] staffing questions I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs.? If anybody is willing to answer the questions listed below, I would appreciate your input.? You may email me at privately and all information will be kept confidental. 1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides 2. Approximate workload, either blocks per day or cases per year 3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful) Thank You in advance Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital ahutton@dh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 103, Issue 7 **************************************** From ree3 <@t> leicester.ac.uk Thu Jun 7 02:43:59 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Jun 7 02:44:25 2012 Subject: [Histonet] Sequenza units for manual immunohistochemistry In-Reply-To: <4FCF3DB1.C0AD.00BF.0@inspection.gc.ca> References: <4FCF39DD020000BF00008D26@inspection.gc.ca> <4FCF3DB1020000BF00008D2F@inspection.gc.ca> <4FCF3DB1.C0AD.00BF.0@inspection.gc.ca> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4FEF6A2AB@EXC-MBX3.cfs.le.ac.uk> We empty them before they get to that stage, as this would be bound to affect the efficient working of the Coverplates. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: 06 June 2012 18:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sequenza units for manual immunohistochemistry Hello Histonet, I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent "waste" in the bottom from all your additions and washes. Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom. Has anyone had any issues using these units? How do people prevent a large volume from building up in the bottom? How are people cleaning their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 ext 5613/5511 Facsimile | T?l?copieur: 403-382-5585 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Jun 7 04:43:41 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 7 04:43:42 2012 Subject: [Histonet] RE: Approval and review of procedures In-Reply-To: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> References: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> Message-ID: As supervisor of Histology, I review and sign off on the SOPs yearly. Any new or altered are final approved by our medical director. The medical director also signs off on the hardcopy manuals each year. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. Sent: Wednesday, June 06, 2012 5:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Approval and review of procedures Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From one_angel_secret <@t> yahoo.com Thu Jun 7 09:01:15 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jun 7 09:01:36 2012 Subject: [Histonet] RE: Approval and review of procedures In-Reply-To: References: <8DEBF8903D1096439BF270B0CAADB2A00166E59B5B@EXCH12.tmh.tmhs> Message-ID: <05293BD5-159F-44DC-BC98-7ADE5C21E10D@yahoo.com> This is what I do as well. However to try and be more specific to her question a HT could do those task if they had the required education and training per CLIA sub part M. In pathology per CAP GEN. 53400 the technical supervisors are usually the medical directors. So often you see direct supervisors who also have to meet certain education and training requirements dealing with the SOP's , signing them and the pathologist signs off on it. What's it boil down to? Education and training per CLIA and we shouldn't assume that having ASCP HT either meets or does not meet CLIA 's guidelines for this. I encourage anyone dealing with regulatory issues read at least sub part M of CLIA even if you are CAP accredited because that's what CAP is anyway. A organization to help us stay CLIA compliant and above. If Ice confused anyone sorry. CLIA is written in legalese. :/ Sent from my iPhone On Jun 7, 2012, at 5:43 AM, Tom McNemar wrote: > As supervisor of Histology, I review and sign off on the SOPs yearly. Any new or altered are final approved by our medical director. The medical director also signs off on the hardcopy manuals each year. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. > Sent: Wednesday, June 06, 2012 5:30 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Approval and review of procedures > > Hello, > I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? > > Judy Sproles CT(ASCP > > Methodist. Leading Medicine. Recognized by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy.crumpton <@t> tuality.org Thu Jun 7 09:10:22 2012 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Thu Jun 7 09:09:00 2012 Subject: [Histonet] Patient requests for tissues Message-ID: I am wondering how other hospital connected histology labs handle requests from patients to get their tissue back. I am especially interested in the placentas. Our pathologists and managers are struggling to come up with a policy that is sensitive to various religions and cultures but keeps everyone safe. Do you allow patients to get back their specimens after the case is signed out or not??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From info <@t> morphisto.de Thu Jun 7 09:08:57 2012 From: info <@t> morphisto.de (Morphisto GmbH) Date: Thu Jun 7 09:09:12 2012 Subject: [Histonet] formaline free fixatives Message-ID: <265A1F55-A2EF-41DF-A0A6-E36D81B78C33@morphisto.de> Hello, has anyone experiences with formaline free fixatives? What do you think about formaline replacements? Are there any recommendations? Best regards Michael From LBUSTAMANTE <@t> cvm.tamu.edu Thu Jun 7 09:15:40 2012 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Thu Jun 7 09:15:48 2012 Subject: [Histonet] Short time Formaldehyde exposure Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB3B9B2@CVMMB02.cvm.tamu.edu> Does anyone knows a Co. in Texas that does on site testing for short time formalin exposure? Short time of 15 min or less. Thank you. Lin Bustamante From dreynold <@t> mdanderson.org Thu Jun 7 09:19:00 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Thu Jun 7 09:19:01 2012 Subject: [Histonet] Block alignment tool Message-ID: <785BBF0C5F49CE41BA74460A43A08F023219DDC2A8@DCPWVMBXC0VS3.mdanderson.edu> Is there a block alignment tool that will work in a cryostat? I work in research and frequently have to go back and recut small pieces of tissue. My Leica allows me to adjust the head but it is very difficult to get the block lined up on the same axis as before. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 From mike <@t> pathview.com Thu Jun 7 09:29:46 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Jun 7 09:30:45 2012 Subject: [Histonet] Slide distribution amongst Pathologists In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FE2E81@SMCMAIL01.somerset-healthcare.com> References: <003701cd4280$8cc1f160$a645d420$@gmx.at> <4BF03F5404EBDE409AF9232DA74B9DED2DEC33D7DF@FWDCWPMSGCMS09.hca.corpad.net> <38667E7FB77ECD4E91BFAEB8D986386324FBA8227A@LRGHEXVS1.practice.lrgh.org> <3AD061FE740D464FAC7BF6B5CFB7570711FE2E81@SMCMAIL01.somerset-healthcare.com> Message-ID: <003501cd44ba$17fb8da0$47f2a8e0$@pathview.com> Good morning, By the way, if anyone wants an LIS perspective, we've been requested to distribute slides according to: 1. slides per case or relative value units per case 2. specimen type 3. pathologist availability for the day 4. requesting facility 5. requesting clinician 6. and of course, 'manual override' I can also tell you when the computer assigns the cases to a pathologist it takes a lot of the political burden off of the accessioning, grossing, and histology personnel. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, June 05, 2012 8:41 AM To: 'Podawiltz, Thomas'; Susan.Walzer@HCAHealthcare.com; gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists We do the same. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, June 05, 2012 6:06 AM To: Susan.Walzer@HCAHealthcare.com; gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists We have one simple way of doing it. He who Grosses is he who reads. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Tuesday, June 05, 2012 3:21 AM To: gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists Boy, they sure like to put us in the middle of what should be their own problem. Thank heavens I only work now for one Dr at a time now but when I was at a larger place they rotated. They still used to tell us to give them particular cases when it was not their turn so that we got the flak when someone did not get what they thought was theirs. You can never win! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, June 04, 2012 2:34 PM To: 'Sheila Adey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Slide distribution amongst Pathologists Here the slides go through the hands of one pathologist, who distributes the cases. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey Gesendet: Montag, 04. Juni 2012 20:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Timothy.Morken <@t> ucsfmedctr.org Thu Jun 7 10:00:10 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jun 7 09:59:58 2012 Subject: [Histonet] RE: Short time Formaldehyde exposure In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB3B9B2@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC95BB3B9B2@CVMMB02.cvm.tamu.edu> Message-ID: <8D7C2D242DBD45498006B21122072BF8B54D43A8@MCINFRWEM003.ucsfmedicalcenter.org> Lin, we use a company called Advanced Chemical Sensors, who will send you badges to be worn by the personnel exposed. They simply fill out a form telling what they did, what protection and controls were used (ie, fume hood) and length of exposure. The tech wears the badge for the specified time period and then you send it back to them for analysis. Very easy to do. Website: http://acsbadge.com/formaldehyde.shtml Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Thursday, June 07, 2012 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Short time Formaldehyde exposure Does anyone knows a Co. in Texas that does on site testing for short time formalin exposure? Short time of 15 min or less. Thank you. Lin Bustamante _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bridget.maryott <@t> ventana.roche.com Thu Jun 7 10:01:25 2012 From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget) Date: Thu Jun 7 10:01:54 2012 Subject: [Histonet] RE: Unsubscribe Me Message-ID: I am making a list of all those who are unqualified to unsubscribe themselves. I will then refer to this this when hiring. I kid, I kid. -Bridget Maryott, HT (ASCP) From SLB <@t> stowers.org Thu Jun 7 10:06:38 2012 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Thu Jun 7 10:06:53 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9A0B65B18@EXCHMB-02.stowers-institute.org> Too funny! But, good idea. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryott, Bridget Sent: Thursday, June 07, 2012 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Unsubscribe Me I am making a list of all those who are unqualified to unsubscribe themselves. I will then refer to this this when hiring. I kid, I kid. -Bridget Maryott, HT (ASCP) From nmhisto <@t> comcast.net Thu Jun 7 11:24:09 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Thu Jun 7 11:24:33 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF9A0B65B18@EXCHMB-02.stowers-institute.org> Message-ID: <380369746.810982.1339086249858.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> This is a very logical way of determining who can follow directions... ----- Original Message ----- From: "Sharon Beckham" To: "Bridget' 'Maryott" , histonet@lists.utsouthwestern.edu Sent: Thursday, June 7, 2012 9:06:38 AM Subject: [Histonet] RE: Unsubscribe Me Too funny! But, good idea. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryott, Bridget Sent: Thursday, June 07, 2012 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Unsubscribe Me I am making a list of all those who are unqualified to unsubscribe themselves. I will then refer to this this when hiring. I kid, I kid. -Bridget Maryott, HT (ASCP) ?? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Thu Jun 7 11:26:20 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Thu Jun 7 11:26:28 2012 Subject: [Histonet] Patient requests for tissues Message-ID: <31f2a449b8d40d8ff4b549d9eac1cf5e@medicalcenterclinic.com> We normally do not allow patients to have their specimens back after they are signed out, but recently we did have a patient request a specimen to be returned due to his religious preferences.? We have held onto the specimen and it's being looked at by our legal and risk management departments.? The biggest issue with?this is?the chemical (formalin) and?biohazard exposure involved.? Eventually, the patient received his specimen, but there was a lot of red tape and it's the only instance in which I have seen a patient get a specimen back after we were done with it. Brendal Finlay, HT ASCP -----Original message----- From: Cathy Crumpton cathy.crumpton@tuality.org Date: Thu, 07 Jun 2012 08:10:22 -0500 To: "histonet@lists.utsouthwestern.edu" histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient requests for tissues > I am wondering how other hospital connected histology labs handle requests from patientsto get their tissue back. I am especially interested in the placentas. Our pathologists and managers are struggling to come up with a policy that is sensitive to various religions and cultures but keeps everyone safe. Do you allow patients to get back their specimens after the case is signed out or not??? > > > > Cathy Crumpton HT(ASCP), Lead Histotechnician > > Tuality Community Hospital > > 503-681-1292 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From one_angel_secret <@t> yahoo.com Thu Jun 7 11:35:06 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jun 7 11:35:21 2012 Subject: [Histonet] Patient requests for tissues In-Reply-To: <31f2a449b8d40d8ff4b549d9eac1cf5e@medicalcenterclinic.com> References: <31f2a449b8d40d8ff4b549d9eac1cf5e@medicalcenterclinic.com> Message-ID: I agree. I had this situation come up as the patient wanted the placenta to eat. I got out of that by explaining that federal law requires us to keep wet specimens for 2 weeks after sign out and that it would have to go in formalin as well. Also once had a patient request her uterus and another their arm I referred both those patients to go through a mortuary service and we sent those over to the service. I don't know what happened between the mortuary and parts after that. Hope this helps. Sent from my iPhone On Jun 7, 2012, at 12:26 PM, "Brendal Finlay" wrote: > > We normally do not allow patients to have their specimens back after > they are signed out, but recently we did have a patient request a > specimen to be returned due to his religious preferences. We have > held onto the specimen and it's being looked at by our legal and risk > management departments. The biggest issue with this is the > chemical (formalin) and biohazard exposure involved. Eventually, > the patient received his specimen, but there was a lot of red tape and > it's the only instance in which I have seen a patient get a specimen > back after we were done with it. > > > Brendal Finlay, HT ASCP > > > -----Original message----- > From: Cathy Crumpton cathy.crumpton@tuality.org > Date: Thu, 07 Jun 2012 08:10:22 -0500 > To: "histonet@lists.utsouthwestern.edu" > histonet@lists.utsouthwestern.edu > Subject: [Histonet] Patient requests for tissues > >> I am wondering how other hospital connected histology labs handle > requests from patientsto get their tissue back. I am especially > interested in the placentas. Our pathologists and managers are > struggling to come up with a policy that is sensitive to various > religions and cultures but keeps everyone safe. Do you allow patients > to get back their specimens after the case is signed out or not??? >> >> >> >> Cathy Crumpton HT(ASCP), Lead Histotechnician >> >> Tuality Community Hospital >> >> 503-681-1292 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Jun 7 13:21:29 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 7 13:20:01 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: <380369746.810982.1339086249858.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> References: <380369746.810982.1339086249858.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <4FD0F129.2030503@umdnj.edu> On 6/7/2012 12:24 PM, nmhisto@comcast.net wrote: > This is a very logical way of determining who can follow directions... IF they could follow directions ... Geoff > > ----- Original Message ----- > From: "Sharon Beckham" > To: "Bridget' 'Maryott", histonet@lists.utsouthwestern.edu > Sent: Thursday, June 7, 2012 9:06:38 AM > Subject: [Histonet] RE: Unsubscribe Me > > Too funny! But, good idea. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryott, Bridget > Sent: Thursday, June 07, 2012 10:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Unsubscribe Me > > I am making a list of all those who are unqualified to unsubscribe themselves. I will then refer to this this when hiring. > > I kid, I kid. > > -Bridget Maryott, HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Mark.Elliott <@t> hli.ubc.ca Thu Jun 7 13:51:00 2012 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Jun 7 13:51:29 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 31 In-Reply-To: References: Message-ID: <4FD095A4020000D60004CC3F@mail.mrl.ubc.ca> I have been asked by a colleague if I know of someplace to buy purified smooth muscle actin. Most places we have looked only have it tagged, but they need untagged as they are looking at it under atomic force microscopy to look at the structure of the filaments and interaction with other proteins. Anyone know of a source. They have tried purifying it themselves from pig trachea with no luck. Thanks Mark in very soggy Vancouver BC ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Ashley.Troutman <@t> Vanderbilt.Edu Thu Jun 7 15:41:21 2012 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Jun 7 15:42:49 2012 Subject: [Histonet] Slide distribution amongst Pathologists Message-ID: <7B310892042DA74CB3590053F424CFE614600C2C03@ITS-HCWNEM06.ds.Vanderbilt.edu> I like Michael's solution. We used to give them out 10 cases at a time as they came off the stainer. Currently, I am in a lab that color codes blocks by service (GI, GYN, ENT, etc.) and these cases are all delivered to one location and whatever pathologist is assigned to that service picks up the cases in the respective box. Hope that helps. Ashley Troutman Message: 3 Date: Thu, 7 Jun 2012 10:29:46 -0400 From: "Michael Mihalik" > Subject: RE: [Histonet] Slide distribution amongst Pathologists To: "'Rathborne, Toni'" >, "'Podawiltz, Thomas'" >, >, >, > Cc: histonet@lists.utsouthwestern.edu Message-ID: <003501cd44ba$17fb8da0$47f2a8e0$@pathview.com> Content-Type: text/plain; charset="UTF-8" Good morning, By the way, if anyone wants an LIS perspective, we've been requested to distribute slides according to: 1. slides per case or relative value units per case 2. specimen type 3. pathologist availability for the day 4. requesting facility 5. requesting clinician 6. and of course, 'manual override' I can also tell you when the computer assigns the cases to a pathologist it takes a lot of the political burden off of the accessioning, grossing, and histology personnel. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, June 05, 2012 8:41 AM To: 'Podawiltz, Thomas'; Susan.Walzer@HCAHealthcare.com; gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists We do the same. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, June 05, 2012 6:06 AM To: Susan.Walzer@HCAHealthcare.com; gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists We have one simple way of doing it. He who Grosses is he who reads. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Tuesday, June 05, 2012 3:21 AM To: gu.lang@gmx.at; sadey@hotmail.ca Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide distribution amongst Pathologists Boy, they sure like to put us in the middle of what should be their own problem. Thank heavens I only work now for one Dr at a time now but when I was at a larger place they rotated. They still used to tell us to give them particular cases when it was not their turn so that we got the flak when someone did not get what they thought was theirs. You can never win! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, June 04, 2012 2:34 PM To: 'Sheila Adey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Slide distribution amongst Pathologists Here the slides go through the hands of one pathologist, who distributes the cases. Gudrun -----Urspr??ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey Gesendet: Montag, 04. Juni 2012 20:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide distribution amongst Pathologists Hello Netters: I am looking for some ideas regarding slide distribution amongst pathologists. Currently one Dr. reads all the surgicals and one other reads the cytology and bone marrows. We now have 5 Dr.s and they are looking for ways to disperse the work evenly per day. Thanks :) Sheila Adey Charge Technologist Laboratory - Histology Department Bluewater Health 89 Norman Street Sarnia, ON N7T 6S3 519-464-4500 x 7063 From njohnson <@t> kcskincenter.com Thu Jun 7 15:55:46 2012 From: njohnson <@t> kcskincenter.com (Nacaela Johnson) Date: Thu Jun 7 15:52:26 2012 Subject: [Histonet] Position opening in KC, Missouri Message-ID: <006c01cd44ef$ea222070$be666150$@com> We are looking for a talented, self sufficient Histotechnologist to run a growing Derm lab in Kansas City, MO. Ideal candidate would have: Associates, preferably Bachelors in science HT or HTL certification 3+ years experience Leadership capabilities This individual must have exceptional communication skills. Experience with Quality Analysis and Documentation procedures is required. Please contact Tammy Clemmer. tclemmer@kcskincenter.com 816-584-8100 From one_angel_secret <@t> yahoo.com Thu Jun 7 19:53:31 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jun 7 19:53:34 2012 Subject: [Histonet] ASP300 issue help? Message-ID: <1339116811.93874.YahooMailNeo@web112312.mail.gq1.yahoo.com> Hi Histonettters.... ? We are having some issues with our ASP300 tissue processor, it seems to be leeking paraffin down into the cleaning xylene causing it to clog and then we get a vacuum error. This is the second time in just over a month. We've had?many valves replaced etc. Has anyone else had this problem? And does anyone have any suggestions for me to check. Would really appreciate your thoughts :) ? Thanks ? Kim D From one_angel_secret <@t> yahoo.com Thu Jun 7 19:56:01 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jun 7 19:56:07 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: <4FD0F129.2030503@umdnj.edu> References: <380369746.810982.1339086249858.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> <4FD0F129.2030503@umdnj.edu> Message-ID: <1339116962.56082.YahooMailNeo@web112304.mail.gq1.yahoo.com> I love it when you guys are funny. I wont unsubscribe <:P ... but sometimes I sure do wish there was a "UNSEND" button. ? :o) ________________________________ From: Geoff McAuliffe To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 7, 2012 2:21 PM Subject: Re: [Histonet] RE: Unsubscribe Me On 6/7/2012 12:24 PM, nmhisto@comcast.net wrote: > This is a very logical way of determining who can follow directions... IF they could follow directions ... Geoff > > ----- Original Message ----- > From: "Sharon Beckham" > To: "Bridget' 'Maryott", histonet@lists.utsouthwestern.edu > Sent: Thursday, June 7, 2012 9:06:38 AM > Subject: [Histonet] RE: Unsubscribe Me > > Too funny! But, good idea. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryott, Bridget > Sent: Thursday, June 07, 2012 10:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Unsubscribe Me > > I am making a list of all those who are unqualified to unsubscribe themselves. I will then refer to this this when hiring. > > I kid, I kid. > > -Bridget Maryott, HT (ASCP) >? ? ? ? ? ? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From techonebs <@t> comcast.net Thu Jun 7 20:03:46 2012 From: techonebs <@t> comcast.net (Matt Mincer) Date: Thu Jun 7 20:04:05 2012 Subject: [Histonet] Re: block alignment tool Message-ID: <4FD14F72.3060105@comcast.net> Hey Donna, We sell a cryostat alignment tool. Check it out at our store http://store.techonebiomedical.com/store/cryostat-alignment-tool.html . If you need an explanation of how to use it, please give me a call. Thanks Matt Mincer TECH ONE BIOMEDICAL SERVICES toll-free 866.497.3033 | office 708.383.6040 | cell 708.822.3738 From talulahgosh <@t> gmail.com Fri Jun 8 08:21:51 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 8 08:22:00 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: <1339116962.56082.YahooMailNeo@web112304.mail.gq1.yahoo.com> References: <380369746.810982.1339086249858.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> <4FD0F129.2030503@umdnj.edu> <1339116962.56082.YahooMailNeo@web112304.mail.gq1.yahoo.com> Message-ID: Don't kid! This is the digital age, how can anyone not know how to unsubscribe themselves from a mailing list?! And speaking of being behind the times, I just found out my university's copying center SCANS hard copies of documents to print them in larger quantities. Apparently, they don't have those newfangled "flash drives" or that "email" the kids are using these days. Somehow this is more efficient for them when you need 150 copies of a 300 page syllabus. I shudder to think of the technology they use (carbon paper? monks who copy with quills?) Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Thu, Jun 7, 2012 at 8:56 PM, Kim Donadio wrote: > I love it when you guys are funny. I wont unsubscribe <:P ... but > sometimes I sure do wish there was a "UNSEND" button. > > :o) > > > ________________________________ > From: Geoff McAuliffe > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, June 7, 2012 2:21 PM > Subject: Re: [Histonet] RE: Unsubscribe Me > > On 6/7/2012 12:24 PM, nmhisto@comcast.net wrote: > > This is a very logical way of determining who can follow directions... > > IF they could follow directions ... > > Geoff > > > > ----- Original Message ----- > > From: "Sharon Beckham" > > To: "Bridget' 'Maryott", > histonet@lists.utsouthwestern.edu > > Sent: Thursday, June 7, 2012 9:06:38 AM > > Subject: [Histonet] RE: Unsubscribe Me > > > > Too funny! But, good idea. > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryott, Bridget > > Sent: Thursday, June 07, 2012 10:01 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: Unsubscribe Me > > > > I am making a list of all those who are unqualified to unsubscribe > themselves. I will then refer to this this when hiring. > > > > I kid, I kid. > > > > -Bridget Maryott, HT (ASCP) > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Fri Jun 8 08:44:59 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Fri Jun 8 08:45:11 2012 Subject: [Histonet] Why people are having issues unsubscribing Message-ID: There are 3 reasons why it is not immediately obvious to people that one of the links at the bottom of Histonet email helps you unsubscribe: 1. In this day and age of internet and email hypertext clutter people simply do not have the time nor the desire to click and explore every link they come across to see which one is going to lead them to the desired page. If the hypertext of a link does not jump out at a person with the info they want, they disregard it and keep looking or freak out and ask the whole list. Neither link at the bottom of Histonet email explicitly states the info most people are probably looking for - that is, to unsubscribe. 2. Most email (junk or subscription) that people receive contain a link that states, "To unsubscribe CLICK HERE," or words to that effect. I believe this is what most people are looking for who are trying to unsubscribe from Histonet. 3. Most people trying to unsubscribe are not even reading well-intentioned email sent from Histonetters trying to help them. They see [Histonet] in the subject line and disregard it because they're trying to unsubscribe, not read more Histonet email. Just trying to facilitate some understanding here of the problem. That said, my suggestion to the list moderator would be to include a link at the bottom of Histonet email that explicitly states "To unsubscribe CLICK HERE." This should reduce some of the clutter of unwanted and unnecessary off-topic (i.e., "unsubscribe me please!!!") Histonet email. Regards, Merced Merced M Leiker Cardiovascular Medicine Biomedical Research Building Rm 348 State University of New York at Buffalo 3435 Main St., Buffalo, NY 14214 (Ph) 716-829-6118 (Fx) 716-829-2665 ______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From veronique.barres <@t> gmail.com Fri Jun 8 09:13:54 2012 From: veronique.barres <@t> gmail.com (=?ISO-8859-1?Q?V=E9ronique_Barr=E8s?=) Date: Fri Jun 8 09:14:33 2012 Subject: [Histonet] microtome Message-ID: Hi histonetters! We are a molecular biology lab with a histology facility (we do whole FFPE tissue sections to build TMAs and then TMA sectionning). We currently have a Leica RM2125 in the lab, but want to acquire a new manual microtome, mainly as a safe because we depend a lot on tissue sectioning. Could anyone give me some advice/suggestion on which microtome to buy? We only use the regular cassettes. Best regards, V?ronique From lpwenk <@t> sbcglobal.net Fri Jun 8 09:14:53 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 8 09:14:58 2012 Subject: [Histonet] Why people are having issues unsubscribing Message-ID: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> Merced, This makes perfect sense. Many people (young and old) are NOT internet savvy and indeed will NOT go searching for the correct way to ?unsubscribe?. Adding a hyperlink to unsubscribe would solve this problem for most people. I'm certain that there are some people that would not see something as obvious as this and would still be asking to be unsubscribed. Lee Wenk -----Original Message----- From: Leiker, Merced Sent: Friday, June 08, 2012 9:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Why people are having issues unsubscribing There are 3 reasons why it is not immediately obvious to people that one of the links at the bottom of Histonet email helps you unsubscribe: 1. In this day and age of internet and email hypertext clutter people simply do not have the time nor the desire to click and explore every link they come across to see which one is going to lead them to the desired page. If the hypertext of a link does not jump out at a person with the info they want, they disregard it and keep looking or freak out and ask the whole list. Neither link at the bottom of Histonet email explicitly states the info most people are probably looking for - that is, to unsubscribe. 2. Most email (junk or subscription) that people receive contain a link that states, "To unsubscribe CLICK HERE," or words to that effect. I believe this is what most people are looking for who are trying to unsubscribe from Histonet. 3. Most people trying to unsubscribe are not even reading well-intentioned email sent from Histonetters trying to help them. They see [Histonet] in the subject line and disregard it because they're trying to unsubscribe, not read more Histonet email. Just trying to facilitate some understanding here of the problem. That said, my suggestion to the list moderator would be to include a link at the bottom of Histonet email that explicitly states "To unsubscribe CLICK HERE." This should reduce some of the clutter of unwanted and unnecessary off-topic (i.e., "unsubscribe me please!!!") Histonet email. Regards, Merced Merced M Leiker Cardiovascular Medicine Biomedical Research Building Rm 348 State University of New York at Buffalo 3435 Main St., Buffalo, NY 14214 (Ph) 716-829-6118 (Fx) 716-829-2665 ______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 8 09:20:23 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 8 09:20:29 2012 Subject: [Histonet] RE: Why people are having issues unsubscribing In-Reply-To: References: Message-ID: Perfect suggestion!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leiker, Merced Sent: Friday, June 08, 2012 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Why people are having issues unsubscribing There are 3 reasons why it is not immediately obvious to people that one of the links at the bottom of Histonet email helps you unsubscribe: 1. In this day and age of internet and email hypertext clutter people simply do not have the time nor the desire to click and explore every link they come across to see which one is going to lead them to the desired page. If the hypertext of a link does not jump out at a person with the info they want, they disregard it and keep looking or freak out and ask the whole list. Neither link at the bottom of Histonet email explicitly states the info most people are probably looking for - that is, to unsubscribe. 2. Most email (junk or subscription) that people receive contain a link that states, "To unsubscribe CLICK HERE," or words to that effect. I believe this is what most people are looking for who are trying to unsubscribe from Histonet. 3. Most people trying to unsubscribe are not even reading well-intentioned email sent from Histonetters trying to help them. They see [Histonet] in the subject line and disregard it because they're trying to unsubscribe, not read more Histonet email. Just trying to facilitate some understanding here of the problem. That said, my suggestion to the list moderator would be to include a link at the bottom of Histonet email that explicitly states "To unsubscribe CLICK HERE." This should reduce some of the clutter of unwanted and unnecessary off-topic (i.e., "unsubscribe me please!!!") Histonet email. Regards, Merced Merced M Leiker Cardiovascular Medicine Biomedical Research Building Rm 348 State University of New York at Buffalo 3435 Main St., Buffalo, NY 14214 (Ph) 716-829-6118 (Fx) 716-829-2665 ______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From brett_connolly <@t> merck.com Fri Jun 8 09:26:54 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jun 8 09:27:20 2012 Subject: [Histonet] Why people are having issues unsubscribing In-Reply-To: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> References: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> Message-ID: I also agree that that adding a specific "Unsubscribe" link would hopefully alleviate the problem. Now....please unscribe, uscribe and unsubscribe me. Hey - it's Friday :) Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, June 08, 2012 10:15 AM To: Histonet Subject: Re: [Histonet] Why people are having issues unsubscribing Merced, This makes perfect sense. Many people (young and old) are NOT internet savvy and indeed will NOT go searching for the correct way to 'unsubscribe'. Adding a hyperlink to unsubscribe would solve this problem for most people. I'm certain that there are some people that would not see something as obvious as this and would still be asking to be unsubscribed. Lee Wenk -----Original Message----- From: Leiker, Merced Sent: Friday, June 08, 2012 9:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Why people are having issues unsubscribing There are 3 reasons why it is not immediately obvious to people that one of the links at the bottom of Histonet email helps you unsubscribe: 1. In this day and age of internet and email hypertext clutter people simply do not have the time nor the desire to click and explore every link they come across to see which one is going to lead them to the desired page. If the hypertext of a link does not jump out at a person with the info they want, they disregard it and keep looking or freak out and ask the whole list. Neither link at the bottom of Histonet email explicitly states the info most people are probably looking for - that is, to unsubscribe. 2. Most email (junk or subscription) that people receive contain a link that states, "To unsubscribe CLICK HERE," or words to that effect. I believe this is what most people are looking for who are trying to unsubscribe from Histonet. 3. Most people trying to unsubscribe are not even reading well-intentioned email sent from Histonetters trying to help them. They see [Histonet] in the subject line and disregard it because they're trying to unsubscribe, not read more Histonet email. Just trying to facilitate some understanding here of the problem. That said, my suggestion to the list moderator would be to include a link at the bottom of Histonet email that explicitly states "To unsubscribe CLICK HERE." This should reduce some of the clutter of unwanted and unnecessary off-topic (i.e., "unsubscribe me please!!!") Histonet email. Regards, Merced Merced M Leiker Cardiovascular Medicine Biomedical Research Building Rm 348 State University of New York at Buffalo 3435 Main St., Buffalo, NY 14214 (Ph) 716-829-6118 (Fx) 716-829-2665 ______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From SStephenson <@t> lifecell.com Fri Jun 8 09:53:29 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Fri Jun 8 09:53:38 2012 Subject: [Histonet] microtome In-Reply-To: References: Message-ID: If you want to stay with Leica; they have a newer model called RM2255 it has the manual and automatic rotations. Sheryl Stephenson | Histology Technician ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s Sent: Friday, June 08, 2012 10:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome Hi histonetters! We are a molecular biology lab with a histology facility (we do whole FFPE tissue sections to build TMAs and then TMA sectionning). We currently have a Leica RM2125 in the lab, but want to acquire a new manual microtome, mainly as a safe because we depend a lot on tissue sectioning. Could anyone give me some advice/suggestion on which microtome to buy? We only use the regular cassettes. Best regards, V?ronique _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Fri Jun 8 10:13:06 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Jun 8 10:13:17 2012 Subject: [Histonet] microtome In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F09298A6074@EXCHMB01.isis.local> Leica 2255, all the way. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s Sent: Friday, June 08, 2012 7:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome Hi histonetters! We are a molecular biology lab with a histology facility (we do whole FFPE tissue sections to build TMAs and then TMA sectionning). We currently have a Leica RM2125 in the lab, but want to acquire a new manual microtome, mainly as a safe because we depend a lot on tissue sectioning. Could anyone give me some advice/suggestion on which microtome to buy? We only use the regular cassettes. Best regards, V?ronique _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri Jun 8 10:40:38 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Jun 8 10:40:46 2012 Subject: [Histonet] Why people are having issues unsubscribing In-Reply-To: References: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> Message-ID: Please unsubscribe me. :) lololololol (just kidding histofriends, I couldn't resist!) Jokingly, Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 8 10:53:59 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 8 10:54:08 2012 Subject: [Histonet] Why people are having issues unsubscribing In-Reply-To: References: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> Message-ID: I would like to be unscribed... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, June 08, 2012 11:41 AM To: Connolly, Brett M Cc: Histonet Subject: Re: [Histonet] Why people are having issues unsubscribing Please unsubscribe me. :) lololololol (just kidding histofriends, I couldn't resist!) Jokingly, Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From tajibade <@t> echd.org Fri Jun 8 11:00:24 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Fri Jun 8 11:00:38 2012 Subject: [Histonet] why people are unsubscribing? In-Reply-To: References: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> Message-ID: I noticed that histonet has been having a lot of people unsubscribing for the past few weeks. Is there any reason why they do this? Because histonet has being a great source knowledge and excellent for the histotechs, cytotechs and even for the pathologists. I will never unsubscribe, I am a life member. Tunde HTL(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, June 08, 2012 10:41 AM To: Connolly, Brett M Cc: Histonet Subject: Re: [Histonet] Why people are having issues unsubscribing Please unsubscribe me. :) lololololol (just kidding histofriends, I couldn't resist!) Jokingly, Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From JMacDonald <@t> mtsac.edu Fri Jun 8 11:02:41 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jun 8 11:03:49 2012 Subject: [Histonet] why people are unsubscribing? In-Reply-To: Message-ID: I always unsubscribe when I go away for vacation so that the histonet is not informed each time that "I am out of the office". Perhaps others do the same. Tunde Ajibade Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2012 09:00 AM To "'histonet-bounces@lists.utsouthwestern.edu'" cc "histonet@lists.utsouthwestern.edu" Subject [Histonet] why people are unsubscribing? I noticed that histonet has been having a lot of people unsubscribing for the past few weeks. Is there any reason why they do this? Because histonet has being a great source knowledge and excellent for the histotechs, cytotechs and even for the pathologists. I will never unsubscribe, I am a life member. Tunde HTL(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, June 08, 2012 10:41 AM To: Connolly, Brett M Cc: Histonet Subject: Re: [Histonet] Why people are having issues unsubscribing Please unsubscribe me. :) lololololol (just kidding histofriends, I couldn't resist!) Jokingly, Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri Jun 8 11:20:25 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Jun 8 11:20:29 2012 Subject: [Histonet] Why people are having issues unsubscribing In-Reply-To: References: <3E6400FD0B374A9FB1ACD206B00FACE3@HP2010> Message-ID: What about untranscribed? Curiously, Jay A. Lundgren, M.S., HTL (ASCP) From Linda.Margraf <@t> cookchildrens.org Fri Jun 8 11:22:58 2012 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Fri Jun 8 11:23:06 2012 Subject: [Histonet] unsubscribing from the list Message-ID: <928719B9EBFA1C4686918B975FF8452801279D@CCHCSMBX04.CCHCS.LDAP> Hi, histonetters. I tried to get a note added to the bottom of the messages so it says "To unsubscribe click here" but the wonderful University IT guy who helps keep the list running says it cannot be done and I am pretty sure he is correct. I also tried to figure out a way but with no luck. For people on the digest version of the list, every digest they receive starts with all the important links. Here's what the header of the digest says, for anyone needing the information: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu By the way, I do remove people (or add them) about once a week or so, when I can get into the server. My new remote access only works some of the time (ahh, the wonders of technology). So if you have an issue, do let me know but it may take a while for me to be able to fix it. Thanks, Linda M Histonet administrator From TJohnson <@t> gnf.org Fri Jun 8 12:04:54 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Jun 8 12:05:00 2012 Subject: [Histonet] Unsubscribing from the list Message-ID: <9F3CFEE76E51B64991C7485270890B400CDAA05A@EX5.lj.gnf.org> Thanks Linda, we appreciate all you do here. I would also like to add that sometimes the amount of emails gets overwhelming to some. Especially when certain subjects get a bit heated. They can go to digest format, but sometimes even that gets overwhelming, especially when you have nested digests into digests. That one eventually tests my patience as well. It isn't unusual to unsubscribe from email lists by hitting reply and putting unsubscribe in the subject or body of the mail. So these folks are just doing what they've always done. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From careerstudio <@t> bellsouth.net Fri Jun 8 12:10:24 2012 From: careerstudio <@t> bellsouth.net (Career Studio) Date: Fri Jun 8 12:10:30 2012 Subject: [Histonet] Histology/IHC Supervisor for clinical reference lab in southern CA Message-ID: <000401cd4599$986151c0$c923f540$@net> Our client is a leading clinical laboratory currently seeking a California-licensed Histology/IHC Supervisor, Monday thru Friday, to oversee operation & administration of several departments. This individual will work closely with pathologists & other medical professionals to effectively meet operation goals, work with tight timelines and ensure that histology staff adheres to these needs. while conducting high quality work. Specimens: slides, blocks, previously cut....some Grossing.....Bone Marrow and Aspirate. Responsibilities will include: . Management of all day-to-day operations technical activities of IHC/Histology/Imaging Department, including staffing, planning, coordination and evaluation . Troubleshooting quality and production issues including stains, processes, systems, and equipment. . Developing and implementing improvements in systems and processes for enhanced efficiency and quality, against corporate and departmental and goals. Candidate must have BA/BS in Biology, Chemistry or related science, with 4+ years of experience in a clinical lab including at least 3 years of supervisory experience. HT (ASCP); and QIHC qualification helpful. Must meet CLIA requirements. In addition, superior organizational and operational skills and the ability to manage multiple priorities and timelines effectively are needed. Qualities in this individual will be: self-motivated, detail oriented, excellent communication skills, and thriving with high degree of responsibility. Based in southern CA, salary/bonus package is competitive to attract top talent, & relocation assistance is offered. Career Studio is a well-established biotechnology search firm focused on the clinical laboratory sectors. Please contact David King at biolabcareers@aol.com for more information & to discuss your career goals. David King Career Studio national search biolabcareers@aol.com 561-738-6363 Visit us on linkedin: http://www.linkedin.com/in/biotechnologyhires From perrintoshia <@t> yahoo.com Fri Jun 8 12:22:39 2012 From: perrintoshia <@t> yahoo.com (Mark Perrin) Date: Fri Jun 8 12:22:42 2012 Subject: [Histonet] Best controls Message-ID: <1339176159.39804.YahooMailNeo@web44703.mail.sp1.yahoo.com> What is the best tissue to use as a control for Trichrome, GMS and Iron stains? ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From jaylundgren <@t> gmail.com Fri Jun 8 12:34:07 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Jun 8 12:34:12 2012 Subject: [Histonet] Best controls In-Reply-To: <1339176159.39804.YahooMailNeo@web44703.mail.sp1.yahoo.com> References: <1339176159.39804.YahooMailNeo@web44703.mail.sp1.yahoo.com> Message-ID: MTS= skin, lung, small intestine, GMS= known positive tissue Fe= liver from a hemochromatosis patient Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) From rosenfeldtek <@t> hotmail.com Fri Jun 8 13:28:42 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Fri Jun 8 13:28:47 2012 Subject: [Histonet] I thought of unsuscribing once... In-Reply-To: References: , Message-ID: a long time ago when I noticed how much histonet mail was filling up my inbox. Then I remembered that the hotmail address where I get histonet posts exists primarily for the purpose of receiving histonet posts! I have two histonet folders on this account--one for questions and one for answers. It's really not that hard to make a dedicated email account for your Histonet mail. Histonet is like a Craigslist pathology geek forum and don't know what I would do without it. Jerry Ricks Research Scientist University of Washington Department of Pathology > To: tajibade@echd.org > From: JMacDonald@mtsac.edu > Date: Fri, 8 Jun 2012 09:02:41 -0700 > Subject: Re: [Histonet] why people are unsubscribing? > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > I always unsubscribe when I go away for vacation so that the histonet is > not informed each time that "I am out of the office". Perhaps others do > the same. > > > > > Tunde Ajibade > Sent by: histonet-bounces@lists.utsouthwestern.edu > 06/08/2012 09:00 AM > > To > "'histonet-bounces@lists.utsouthwestern.edu'" > > cc > "histonet@lists.utsouthwestern.edu" > Subject > [Histonet] why people are unsubscribing? > > > > > > > I noticed that histonet has been having a lot of people unsubscribing for > the past few weeks. Is there any reason why they do this? Because histonet > has being a great source knowledge and excellent for the histotechs, > cytotechs and even for the pathologists. I will never unsubscribe, I am a > life member. > > Tunde HTL(ASCP)QIHC > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay > Lundgren > Sent: Friday, June 08, 2012 10:41 AM > To: Connolly, Brett M > Cc: Histonet > Subject: Re: [Histonet] Why people are having issues unsubscribing > > Please unsubscribe me. > > :) lololololol (just kidding histofriends, I couldn't resist!) > > Jokingly, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > CONFIDENTIALITY NOTICE: The documents accompanying this email transmission > contain confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any other > party and is required to destroy the information after its stated need has > been fulfilled. If you are not the intended recipient, you are hereby > notified that any disclosure, copying, distribution, or action taken in > reliance on the contents of these documents is strictly prohibited. If you > have received this email in error, please notify the sender immediately to > arrange for return of these documents. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Fri Jun 8 18:43:29 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Jun 8 18:43:44 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 06/08/2012 and will not return until 06/14/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, IHC/Histo issues Kiran at 8-421-5404, or general histology client service at 8-421-5408. From akemiat3377 <@t> yahoo.com Sat Jun 9 10:12:54 2012 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sat Jun 9 10:13:00 2012 Subject: [Histonet] Akemi's Email contacts Hi-Jacked Message-ID: Hello All: Sorry, but it looks like someone hi-jacked my email addresses.... Not sure how this happened last night. Could have been from my LinkedIn, but I changed my password 2 days ago. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From akemiat3377 <@t> yahoo.com Sat Jun 9 10:40:17 2012 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sat Jun 9 10:40:20 2012 Subject: [Histonet] SPAM Sent-Akemi's Email Addresses Hi-Jacked Message-ID: <14731389-AF5C-4731-B8A5-F221FE812A27@yahoo.com> Hello All: SPAM Sent! Sorry, but it looks like someone hi-jacked my email contact addresses.... I have a MAC, but it could have been from my LinkedIn or my Facebook. I changed my password 2 days ago on my LinkedIn after the notification of a security breach. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From talulahgosh <@t> gmail.com Sat Jun 9 13:22:04 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Jun 9 13:22:08 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: <7D2CD4D6F76D6143999DB4BD2515A0F738B886@hsc1msmx0017.ad.wrha.mb.ca> References: <7D2CD4D6F76D6143999DB4BD2515A0F738B886@hsc1msmx0017.ad.wrha.mb.ca> Message-ID: That's it, I have had it with these Mister Falcon unsubscribes on this Mother Father list!! We need to start screening, using a quiz that involves three questions: 1. How do you unsubscribe from the Histonet list? 2. Describe in less than 100 words how one would stop receiving emails from the Histonet list. 3. If you wanted to remove yourself from the Histonet list, which of the following is the CORRECT way to do so: A. Email everyone on the Histonet list a request to unsubscribe. B. Go to the page linked at the BOTTOM OF EVERY HISTONET EMAIL and follow the directions. C. Email everyone on the Histonet list a request to unsubscribe. D. Email everyone on the Histonet list a request to unsubscribe. E. Email everyone on the Histonet list a request to unsubscribe. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Fri, Jun 8, 2012 at 9:25 AM, Lisa Manning wrote: > ** > > -= Sent from my Blackberry Wireless Handheld =- > > > > From talulahgosh <@t> gmail.com Sat Jun 9 13:39:36 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Jun 9 13:39:41 2012 Subject: [Histonet] the case of the disappearing dna Message-ID: This isn't really histology, more molecular biology, but I'm not aware of any good molecular biology lists ^_^ I recently started making RNA probes again (after about a year of not doing so). When I linearize plasmid DNA (usually pBS, smaller than 4kb total) and run it on a gel, the band is really really light. I know the DNA I'm starting with has a bright band when it's run uncut. The protocol is a basic restriction enzyme digest for 2-3 hours, which I then clean with a Qiagen kit. I've tried running the DNA on a gel before I clean it, in case it's the cleaning which is not working well, but it's still really light. The weird thing is, if I use it to make an RNA probe, I get a pretty big RNA pellet when I ethanol extract it. It's also specific when it's used in a slide in situ. But again, I can't see any RNA on a gel AND the DNA band is very light. WTF is going on?? One thing I haven't checked is the temperature of the water bath--it's a digital reading and over ten years old so it might be off. I have tried different plasmids and different enzymes only to get the same result. When I used to make RNA probes, both the linearized DNA and DNA with RNA probe were pretty bright. Also, I tried different loading buffer and ethidium bromide for the gels. Also also, I've run uncut DNA on the same gel and it was a very bright band. Would it be worth it to take samples of the restriction digest before it's incubated, and then at one hour intervals, to run on a gel with the uncut DNA? I really have no solution for this, and using the RNA for an in situ without seeing it on a gel makes me very nervous. Sorry for the rambling nature of this email, it's Saturday and I keep thinking random things I should mention about what I'm doing. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh From enrriq88 <@t> yahoo.com Sat Jun 9 13:40:32 2012 From: enrriq88 <@t> yahoo.com (enrriq88@yahoo.com) Date: Sat Jun 9 13:40:35 2012 Subject: Fw: [Histonet] RE: Unsubscribe Me In-Reply-To: References: <7D2CD4D6F76D6143999DB4BD2515A0F738B886@hsc1msmx0017.ad.wrha.mb.ca> Message-ID: <1339267232.49553.YahooMailNeo@web113505.mail.gq1.yahoo.com> Unsubcribe Me Now...!!!!!! ----- Forwarded Message ----- From: Emily Sours To: Lisa Manning ; histonet@lists.utsouthwestern.edu Sent: Saturday, June 9, 2012 2:22 PM Subject: Re: [Histonet] RE: Unsubscribe Me That's it, I have had it with these Mister Falcon unsubscribes on this Mother Father list!! We need to start screening, using a quiz that involves three questions: 1. How do you unsubscribe from the Histonet list? 2. Describe in less than 100 words how one would stop receiving emails from the Histonet list. 3. If you wanted to remove yourself from the Histonet list, which of the following is the CORRECT ? ? way to do so: ? ? ? A. Email everyone on the Histonet list a request to unsubscribe. ? ? ? B. Go to the page linked at the BOTTOM OF EVERY HISTONET EMAIL and follow the ? ? ? ? ? directions. ? ? ? C. Email everyone on the Histonet list a request to unsubscribe. ? ? ? D. Email everyone on the Histonet list a request to unsubscribe. ? ? ? E. Email everyone on the Histonet list a request to unsubscribe. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Fri, Jun 8, 2012 at 9:25 AM, Lisa Manning wrote: > ** > > -= Sent from my Blackberry Wireless Handheld =- > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Sat Jun 9 13:59:29 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Jun 9 13:59:33 2012 Subject: [Histonet] Your Request to Histonet Message-ID: Dear enrriq88@yahoo.com, It is with great dismay that I must announce your immediate removal from the internet. Please return your computer, smartphone, and any unused portions of the internet to your local provider. You will not be able to connect again without reading the explicit instructions contained here http://lists.utsouthwestern.edu/mailman/listinfo/histonet The internet would like to thank you for attempting an association with your peers, but you have been deemed unable to utilize this knowledge in an intelligent fashion. The internet is not responsible for any damage that may occur due to misuse. Use caution when operating the internet while under the influence of drugs and/or alcohol. The internet cannot be held accountable for the execution of Rule 34 and/or godwinning. Offer void where prohibited by law. Sorry, Tennessee! Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Sat, Jun 9, 2012 at 2:40 PM, wrote: > Unsubcribe Me Now...!!!!!! > > > ----- Forwarded Message ----- > From: Emily Sours > To: Lisa Manning ; > histonet@lists.utsouthwestern.edu > Sent: Saturday, June 9, 2012 2:22 PM > Subject: Re: [Histonet] RE: Unsubscribe Me > > That's it, I have had it with these Mister Falcon unsubscribes on this > Mother Father list!! > We need to start screening, using a quiz that involves three questions: > > 1. How do you unsubscribe from the Histonet list? > 2. Describe in less than 100 words how one would stop receiving emails from > the Histonet list. > 3. If you wanted to remove yourself from the Histonet list, which of the > following is the CORRECT > way to do so: > > A. Email everyone on the Histonet list a request to unsubscribe. > B. Go to the page linked at the BOTTOM OF EVERY HISTONET EMAIL and > follow the > directions. > C. Email everyone on the Histonet list a request to unsubscribe. > D. Email everyone on the Histonet list a request to unsubscribe. > E. Email everyone on the Histonet list a request to unsubscribe. > > > Emily > > "You see a peanut, day's off to a good start; you witness some soil it's a > jamboree for Vince Noir." > --Howard Moon, in "Charlie", The Mighty Boosh > > > > On Fri, Jun 8, 2012 at 9:25 AM, Lisa Manning > wrote: > > > ** > > > > -= Sent from my Blackberry Wireless Handheld =- > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shirleyennis57 <@t> yahoo.com Sat Jun 9 14:58:27 2012 From: shirleyennis57 <@t> yahoo.com (Shirley Ennis) Date: Sat Jun 9 14:58:37 2012 Subject: [Histonet] QIHC exam Message-ID: What study guides are suggested for QIHC exam ? Sent from my iPhone From gayle.callis <@t> bresnan.net Sat Jun 9 16:10:25 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sat Jun 9 16:10:49 2012 Subject: [Histonet] Re: Unsubscribing problems for the amusement with a touch of seriousness for all Message-ID: <000201cd4684$4bba7270$e32f5750$@bresnan.net> Dear Histonetters particularly those irritated with people who incorrectly unsubscribe from this list, After participating on Histonet since its very beginning, it is noted that incorrect unsubscribing (and other infractions) from the list has been a continuous problem from the start. This has been the proverbial thorn in peoples' sides and always eliciting a rash of How to unsubscribe correctly messages. This rash of "How To Unsubscribe" messages can be found in Histonet Archives for every year this list has been in existence. This includes to the amusement of some, the misspelling of "UNSUBSCRIBE" in interesting, amusing ways. Another irritation is Re: Daily Digest instead of a valid topic in subject line. All these things use to get my lab coat in a bunch, decision were made to calm ruffled feathers and deal with these life's less than important crises. So the following suggestions/comments might help. 1. Don't waste time informing those few people "How To Unsubscribe". This is a lost cause, a proven fact shown not to work during all the years of Histonet. 2. If one is subscribed to Histonet and a subject line contains "Unsubcribe me; Remove me from list; Take me off Histonet, etc." and Re: Daily Digest. Use delete key. 3. Suggestion. If one finds these irritations too difficult to deal with, causing indigestion, clenched jaw syndrome, gnashing of teeth, tension headache, sleep loss , computer keyboard abuse, bad language habits, and Histonet eRoad rage leading a message for all to read, try cruising Histonet Archives daily. You pass up subject(s) not interesting to you, and the OMG not again (!) incorrect "Unsubscribe" practice. When a message/topic piques your interest enough to pen a reply, then hop back onto (whoops! subscribe to) Histonet to share your histo-expertise. Well I am off to unsubscribe correctly and will look for your replies with commentary in the Archives. Gayle M. Callis J HTL/HT/MT (ASCP) From rsrichmond <@t> gmail.com Sat Jun 9 21:47:22 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jun 9 21:47:25 2012 Subject: [Histonet] Re: best controls Message-ID: What controls for trichrome, methenamine silver fungal stain, and hemosiderin? For trichrome, whatever keeps the regulatory agencies happy. Pathologists use it mostly for liver biopsies, so an autopsy liver with early hepatic fibrosis would be ideal. But I don't bother to look at the control. GMS methenamine silver for fungi: I'd want histoplasma growing in human tissue, with numerous yeast forms with a history of bad staining. But you take what you can get. A Candida culture injected into a mouse lung is what I expect to see. Hemosiderin: any tissue with hemosiderin in it. Dog spleen or liver works quite well - unlike people, dogs store a lot of iron. Human hemochromatotic tissue is hard to get, and I'd hope increasingly difficult. Could I add amyloid? The only control I can usually get is human medullary thyroid carcinoma. Amyloidosis is easily induced in experimental animals, and I've asked on Histonet several times - why isn't this tissue available for clinical use? And acid-fast? Once again, I expect mouse lung injected with AFB. The best I've ever seen was rhesus monkey lung, those pestiferous New Delhi monkeys come to autopsy. Leprosy requires a separate AFB control. Once again, why not animal material. Leprosy is easily produced in armadillos (Dasypus novemcinctus), and one lepradillo could supply the world for a century. Bob Richmond Samurai Pathologist Knoxville TN From mayallf <@t> wave.co.nz Sun Jun 10 06:35:00 2012 From: mayallf <@t> wave.co.nz (Dr Mayall) Date: Sun Jun 10 06:35:07 2012 Subject: [Histonet] Free Diagnostic Pathology Software from the UK Message-ID: Some of the list members might be interested in some free diagnostic pathology software that we have developed in the UK. You can view it and download it from this website: www.freedp.org Many histopathology laboratories in the UK are using antiquated reporting software. Upgrading to a more modern system is expensive. Even the more modern systems often lack the technology needed for pathologists to efficiently report complex cases. They are often difficult to use across multiple sites. We have developed reporting software that overcomes these difficulties. This has been trialled by 15 staff at two sites to report over 6000 cases; many complex tumour cases requiring key data element capture. We intend to share this open source software internationally. Over the next year we are planning to incorporate more proforma reports to cover most major tumour types. From one_angel_secret <@t> yahoo.com Sun Jun 10 08:14:35 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sun Jun 10 08:15:33 2012 Subject: [Histonet] RE: Unsubscribe Me In-Reply-To: <1339267232.49553.YahooMailNeo@web113505.mail.gq1.yahoo.com> References: <7D2CD4D6F76D6143999DB4BD2515A0F738B886@hsc1msmx0017.ad.wrha.mb.ca> <1339267232.49553.YahooMailNeo@web113505.mail.gq1.yahoo.com> Message-ID: You can unsubscribe, but you can never leave........Hotel Histonet. The Eagles! Sent from my iPhone On Jun 9, 2012, at 2:40 PM, enrriq88@yahoo.com wrote: > Unsubcribe Me Now...!!!!!! > > > ----- Forwarded Message ----- > From: Emily Sours > To: Lisa Manning ; histonet@lists.utsouthwestern.edu > Sent: Saturday, June 9, 2012 2:22 PM > Subject: Re: [Histonet] RE: Unsubscribe Me > > That's it, I have had it with these Mister Falcon unsubscribes on this > Mother Father list!! > We need to start screening, using a quiz that involves three questions: > > 1. How do you unsubscribe from the Histonet list? > 2. Describe in less than 100 words how one would stop receiving emails from > the Histonet list. > 3. If you wanted to remove yourself from the Histonet list, which of the > following is the CORRECT > way to do so: > > A. Email everyone on the Histonet list a request to unsubscribe. > B. Go to the page linked at the BOTTOM OF EVERY HISTONET EMAIL and > follow the > directions. > C. Email everyone on the Histonet list a request to unsubscribe. > D. Email everyone on the Histonet list a request to unsubscribe. > E. Email everyone on the Histonet list a request to unsubscribe. > > > Emily > > "You see a peanut, day's off to a good start; you witness some soil it's a > jamboree for Vince Noir." > --Howard Moon, in "Charlie", The Mighty Boosh > > > > On Fri, Jun 8, 2012 at 9:25 AM, Lisa Manning wrote: > >> ** >> >> -= Sent from my Blackberry Wireless Handheld =- >> >> >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwray78 <@t> gmail.com Sun Jun 10 13:43:37 2012 From: jwray78 <@t> gmail.com (Josh Wray) Date: Sun Jun 10 13:43:41 2012 Subject: [Histonet] Re: QIHC exam Message-ID: You can't beat the Dako IHC info from their site: http://www.dako.com/us/08002_ihc_staining_methods_5ed.pdf This was my main source of information I used while studying for the exam. Also, look into IHC for cytology prepared specimens online. I had a few of them pop up on the test. Hope this helps! Good luck! Joshua Wray HT(ASCP), QIHC From kc <@t> ka-recruiting.com Sun Jun 10 20:41:54 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Sun Jun 10 20:41:59 2012 Subject: [Histonet] Lead Histotech Job Message-ID: <991177983.1339378914297.JavaMail.cfservice@sl4app2> Dear Histonet Community, I am a one of the founders of a nationally recognized healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in taking the next step in your career. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Lead Histotech position with a hospital outside of Columbus, Ohio. This is a 1st shift Lead Histotechnologist position at one of Eastern Ohio's most highly regarded hospitals. Recognized for superior patient satisfaction this 300 bed, level 1 trauma center hospital offers an excellent work environment. This is a position offers a terrific compensation package; including competitive hourly rate, relocation assistance, and retirement plan. To qualify you must have an AS or BS in Histology and be either HT or HTL (ASCP) certified. Working knowledge of Tissue Processors, Micrometers, Immunohistochemical and Special Staining Instrumentation is required. If you are interested in learning more about this position, please call or e-mail me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email with a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. Histotech/Cytotech Openings: * AZ - Histotech * FL - Treasure Coast - Histotech * PA - Philadelphia - IHC Tech * IN - Histotech * ME - Lead Surgical Pathologist * NC - Histology Supervisor 2nd shift * NC - Histology Manager * NC - Histotech 3rd shift (experienced) * KY - Histotechnologist - 3rd shift * NY - Western - Histotech * NY - NYC - Histotech * NYC - Pathology Manager (commercial background) * OH - Columbus - Histotech - 1st shift * NV - Las Vegas - Histotech, IHC * CA - Southern - Histology Manager To view additional opportunities please visit our website at www.ka-recruiting.com. I look forward to hearing from you. KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From jaylundgren <@t> gmail.com Mon Jun 11 00:33:33 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jun 11 00:33:39 2012 Subject: [Histonet] Re: best controls In-Reply-To: References: Message-ID: I wonder what happened to all the leprous armadillos living at AFIP? Heeere little feller.... Jay A. Lundgren, M.S., HTL (ASCP) From SDattili <@t> stormontvail.org Mon Jun 11 07:42:53 2012 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Mon Jun 11 07:43:03 2012 Subject: [Histonet] RE: Unsubscribing to the list plus generous use of the DELETE function In-Reply-To: <8cafcea100025095@stormontvail.org> Message-ID: Hi all, Teri wrote about how overwhelming it can be when you get the digest form of the list, as there are layers upon layers of the same messages repeated over and over. Please (I beg you) delete all the messages underneath when you use "reply" in your email application to post to the board. It only takes a few moments and would save all of us time and allow us to find that pearl of wisdom that was formerly buried under 7 copies of the same post-reply chain. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From Pathrm35 <@t> comcast.net Mon Jun 11 07:49:07 2012 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Mon Jun 11 07:49:22 2012 Subject: [Histonet] cytology control slides? Message-ID: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> ?Fellow techs, ? I was wondering what some of you do for a control slide ?for your cytology stainer. We will be staining urine cytology specifically. ? Thanks, Ron Martin From asmith <@t> mail.barry.edu Mon Jun 11 08:23:59 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Jun 11 08:24:10 2012 Subject: [Histonet] topoisomerase IIalpha Message-ID: I would like to hear suggestions of a good primary antibody to human topoisomerase IIalpha. From one_angel_secret <@t> yahoo.com Mon Jun 11 09:39:45 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Jun 11 09:39:59 2012 Subject: [Histonet] cytology control slides? In-Reply-To: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: What I used to do was when I got a thick specimen we would prepare 20-30 slides and run one of those each day for a positive control. We also ran a blank slide as a negative control to insure we didn't have contamination in our reagents. Doing these two things were part of our lab to meet CAP guidelines. Sent from my iPhone On Jun 11, 2012, at 8:49 AM, Pathrm35@comcast.net wrote: > > > Fellow techs, > > > > I was wondering what some of you do for a control slide for your cytology stainer. We will be staining urine cytology specifically. > > > > Thanks, > > Ron Martin > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Mon Jun 11 09:42:24 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Jun 11 09:42:38 2012 Subject: [Histonet] QIHC exam In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F09298A607B@EXCHMB01.isis.local> DAKO IHC guide book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Ennis Sent: Saturday, June 09, 2012 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC exam What study guides are suggested for QIHC exam ? Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Mon Jun 11 09:48:15 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Mon Jun 11 09:48:26 2012 Subject: [Histonet] cytology control slides? In-Reply-To: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <7EAFE982E328304DA6CE2B677BB7624639D653B9@TN001WEXMBX11.US.chs.net> We make a buckle smear and stain it. Quick and easy. The QC is for the pap stain quality. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, June 11, 2012 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytology control slides? ?Fellow techs, ? I was wondering what some of you do for a control slide ?for your cytology stainer. We will be staining urine cytology specifically. ? Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From GaleL <@t> unionhospital.org Mon Jun 11 10:26:43 2012 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Mon Jun 11 10:26:53 2012 Subject: [Histonet] giemsa stain control Message-ID: Good Morning! Does anyone have any extra giemsa stain control tissue that they would be willing to share? Thanks! Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From rjbuesa <@t> yahoo.com Mon Jun 11 10:39:41 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 11 10:39:44 2012 Subject: [Histonet] giemsa stain control In-Reply-To: References: Message-ID: <1339429181.52681.YahooMailNeo@web121403.mail.ne1.yahoo.com> Why would you need a "Giemsa control"? ANY tissue, but especially tonsil or bone marrow section, will stain with Giemsa if done properly. It is not a tissue you will need, but a correct procedure that will stain properly a test tissue. Ren? J.. ________________________________ From: Gale Limron To: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 11, 2012 11:26 AM Subject: [Histonet] giemsa stain control Good Morning! Does anyone have any extra giemsa stain control tissue that they would be willing to share? Thanks! Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mdpraet <@t> gmail.com Mon Jun 11 11:07:05 2012 From: mdpraet <@t> gmail.com (mequita praet) Date: Mon Jun 11 11:07:13 2012 Subject: [Histonet] consulting Message-ID: Hi All, I have my own consulting company now. Histology ProConculting, LLC. I set up histology, derm or Mohs labs. I have 40 years+ experience with 20 years of it in derm labs. I have written procedure manuals and kept them CLIA compliant. I can train Mohs techs. I have completed my last project and am looking for my next one. If you need help or know of someone who needs help, I would appreciate your referral. Thanks so much, Mequita Praet mdpraet@gmail.com From kmerriam2003 <@t> yahoo.com Mon Jun 11 11:37:13 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Jun 11 11:37:16 2012 Subject: [Histonet] endogenous peroxidase in eosinophils Message-ID: <1339432633.47254.YahooMailNeo@web130103.mail.mud.yahoo.com> Anyone have tips for quenching endogenous peroxidase in eosinophils?? Our standard px block is not doing the job (Biocare Peroxidazed-1). Thanks, Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From POWELL_SA <@t> mercer.edu Mon Jun 11 11:54:19 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Jun 11 11:54:28 2012 Subject: [Histonet] Re: best controls In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE24B37931C83@MERCERMAIL.MercerU.local> I want to know what happened to all the equipment there, especially the large Sartorius Microtome that was in the bone lab. If anyone has that information please email me. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Monday, June 11, 2012 1:34 AM To: Bob Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: best controls I wonder what happened to all the leprous armadillos living at AFIP? Heeere little feller.... Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 11 12:02:45 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 11 12:02:51 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: <9BF995BC0E47744E9673A41486E24EE24B37931C83@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE24B37931C83@MERCERMAIL.MercerU.local> Message-ID: <1339434165.66848.YahooMailNeo@web121405.mail.ne1.yahoo.com> What happened to the armadillos in AFIP? Who knows what happened to them when AFIP CLOSED! Didn't you know that? Ren? J. ________________________________ From: Shirley A. Powell To: Jay Lundgren ; Bob Richmond Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 11, 2012 12:54 PM Subject: RE: [Histonet] Re: best controls I want to know what happened to all the equipment there, especially the large Sartorius Microtome that was in the bone lab.? If anyone has that information please email me.? Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Monday, June 11, 2012 1:34 AM To: Bob Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: best controls I wonder what happened to all the leprous armadillos living at AFIP? ? ? ? ? ? ? ? Heeere little feller.... ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jun 11 12:09:01 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 11 12:09:11 2012 Subject: [Histonet] RELIA Hot Histology Job Alert Exciting New Opportunities. Message-ID: <027201cd47f4$e61cc590$b25650b0$@earthlink.net> Hi Histonetters! I hope everybody is having a great start to the week. I have some new positions to tell you about. All of the clients that I represent offer full time permanent positions with excellent pay and benefits & They are ready to interview and hire right away! Here are my newest and most exciting opportunities! Lead Histotech - Days near Columbus *Histology Tech - Nights Lafayette, LA *Electron Microscopy Specialist - Long Island, NY *Flow Cytometry Tech- Nashville, TN *Cytogenetics Tech - Nashville, TN * Specialist - Long Island, NY *Night Shift Supervisor - Aurora, IL **I also have some exciting opportunities in KY, PA and NY. If you or anyone you know might be interested in hearing more about any of these opportunities please contact me right away. I can be reached toll free at 866-607-3542 until 6p EST and anytime on my cell at 407-353-5070 or via email at relia1@earthlink.net. There could be a great opportunity here for you. OR you could help out a friend and earn a referral fee from RELIA. Thanks for taking the time to read this email. Have a great day! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From clb1158 <@t> yahoo.com Mon Jun 11 12:33:31 2012 From: clb1158 <@t> yahoo.com (C B) Date: Mon Jun 11 12:33:34 2012 Subject: [Histonet] Histology Intern Positions in Atlanta Message-ID: <1339436011.6434.YahooMailNeo@web114011.mail.gq1.yahoo.com> Are there any labs in the Atlanta area that offer Intern positions in paraffin histology?? After graduating from a formal histology program I worked in electron microscopy then moved on to calcified bone and implants, therefore, all of my working experience has been with plastic resins. I would like to get back in to paraffin and think interning would be a great start. Cindy Baranowski, HT(ASCP) From TNMayer <@t> mdanderson.org Mon Jun 11 13:49:34 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Jun 11 13:50:52 2012 Subject: [Histonet] RE: best controls (Bob Richmond) Message-ID: For trichrome: appendix is the most universal control I know of. Then maybe a kidney. GMS: store bought controls are nice, but if you live close to the local morgue try them, every so often they get a good case that they can spare some. Hemosiderin: Fetal Liver is good, or as you say dog. Call the vet or vet school and see if they can get you some. Also ask them for some mast cell tissue. Dogs get mast cells and they stain beautifully. Amyloid: the problem is with the storage, just freeze the block after you cut it. One block should last you for years. Also contact the closest amyloidosis clinic and see if they can find a place to supply a block. They would know of positive cases. AFB/Fite: get in touch with Hansen's disease center, they can provide you with a block 'free' of charge. Message: 7 Date: Sat, 9 Jun 2012 22:47:22 -0400 From: Bob Richmond Subject: [Histonet] Re: best controls To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 What controls for trichrome, methenamine silver fungal stain, and hemosiderin? For trichrome, whatever keeps the regulatory agencies happy. Pathologists use it mostly for liver biopsies, so an autopsy liver with early hepatic fibrosis would be ideal. But I don't bother to look at the control. GMS methenamine silver for fungi: I'd want histoplasma growing in human tissue, with numerous yeast forms with a history of bad staining. But you take what you can get. A Candida culture injected into a mouse lung is what I expect to see. Hemosiderin: any tissue with hemosiderin in it. Dog spleen or liver works quite well - unlike people, dogs store a lot of iron. Human hemochromatotic tissue is hard to get, and I'd hope increasingly difficult. Could I add amyloid? The only control I can usually get is human medullary thyroid carcinoma. Amyloidosis is easily induced in experimental animals, and I've asked on Histonet several times - why isn't this tissue available for clinical use? And acid-fast? Once again, I expect mouse lung injected with AFB. The best I've ever seen was rhesus monkey lung, those pestiferous New Delhi monkeys come to autopsy. Leprosy requires a separate AFB control. Once again, why not animal material. Leprosy is easily produced in armadillos (Dasypus novemcinctus), and one lepradillo could supply the world for a century. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ From Pathrm35 <@t> comcast.net Mon Jun 11 14:24:37 2012 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Mon Jun 11 14:24:53 2012 Subject: [Histonet] Uro-2 controls Message-ID: <546808344.1610493.1339442677709.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Does anyone know of a company that sells Uro-2 control slides? From bpineau <@t> cellmarque.com Mon Jun 11 14:40:18 2012 From: bpineau <@t> cellmarque.com (Brandon Pineau) Date: Mon Jun 11 14:40:28 2012 Subject: [Histonet] Uro-2 controls In-Reply-To: <546808344.1610493.1339442677709.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <546808344.1610493.1339442677709.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <80005D45618C0A46972477C18124C2AC4145C808@cmrocmb1.cellmarque.local> Cell Marque sells them....part number 345S. Thank you! Brandon Pineau -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, June 11, 2012 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uro-2 controls Does anyone know of a company that sells Uro-2 control slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmhisto <@t> comcast.net Mon Jun 11 14:53:17 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Mon Jun 11 14:53:39 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: <1339434165.66848.YahooMailNeo@web121405.mail.ne1.yahoo.com> Message-ID: <1668253677.963578.1339444397313.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Rene - Those were STUFFED armadillos that had escaped from the Smithsonian!? The real armadillo work (with leprosy) is still going on in Hawaii ('cause I got involved in that for a very short period of time in the Olde Days).? If you want an armadillo - ask someone that lives in Texas and I'm sure they'd be happy to round one up for you.? Watch them claws, though. Retirement... aaah...time to annoy people! ----- Original Message ----- From: "Rene J Buesa" To: "Shirley A. Powell" , "Jay Lundgren" , "Bob Richmond" Cc: histonet@lists.utsouthwestern.edu Sent: Monday, June 11, 2012 11:02:45 AM Subject: Re: [Histonet] Armadillos living at AFIP What happened to the armadillos in AFIP? Who knows what happened to them when AFIP CLOSED! Didn't you know that? Ren? J. ________________________________ From: Shirley A. Powell To: Jay Lundgren ; Bob Richmond Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 11, 2012 12:54 PM Subject: RE: [Histonet] Re: best controls I want to know what happened to all the equipment there, especially the large Sartorius Microtome that was in the bone lab.? If anyone has that information please email me.? Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Monday, June 11, 2012 1:34 AM To: Bob Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: best controls I wonder what happened to all the leprous armadillos living at AFIP? ? ? ? ? ? ? ? Heeere little feller.... ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Jun 11 15:08:43 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jun 11 15:08:51 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: <1668253677.963578.1339444397313.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> References: <1339434165.66848.YahooMailNeo@web121405.mail.ne1.yahoo.com> <1668253677.963578.1339444397313.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: Is that what you get to do in retirement, I am working on my second one, hopefully I can start ignoring now............. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Monday, June 11, 2012 1:53 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Bob Richmond; Shirley A. Powell Subject: Re: [Histonet] Armadillos living at AFIP Rene - Those were STUFFED armadillos that had escaped from the Smithsonian!? The real armadillo work (with leprosy) is still going on in Hawaii ('cause I got involved in that for a very short period of time in the Olde Days).? If you want an armadillo - ask someone that lives in Texas and I'm sure they'd be happy to round one up for you.? Watch them claws, though. Retirement... aaah...time to annoy people! ----- Original Message ----- From: "Rene J Buesa" To: "Shirley A. Powell" , "Jay Lundgren" , "Bob Richmond" Cc: histonet@lists.utsouthwestern.edu Sent: Monday, June 11, 2012 11:02:45 AM Subject: Re: [Histonet] Armadillos living at AFIP What happened to the armadillos in AFIP? Who knows what happened to them when AFIP CLOSED! Didn't you know that? Ren? J. ________________________________ From: Shirley A. Powell To: Jay Lundgren ; Bob Richmond Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 11, 2012 12:54 PM Subject: RE: [Histonet] Re: best controls I want to know what happened to all the equipment there, especially the large Sartorius Microtome that was in the bone lab.? If anyone has that information please email me.? Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Monday, June 11, 2012 1:34 AM To: Bob Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: best controls I wonder what happened to all the leprous armadillos living at AFIP? ? ? ? ? ? ? ? Heeere little feller.... ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 11 15:08:52 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 11 15:08:57 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: <1668253677.963578.1339444397313.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> References: <1339434165.66848.YahooMailNeo@web121405.mail.ne1.yahoo.com> <1668253677.963578.1339444397313.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <1339445332.71519.YahooMailNeo@web121404.mail.ne1.yahoo.com> Thanks! Nice to know. Ren? J. ________________________________ From: "nmhisto@comcast.net" To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Shirley A. Powell ; Jay Lundgren ; Bob Richmond Sent: Monday, June 11, 2012 3:53 PM Subject: Re: [Histonet] Armadillos living at AFIP Rene - Those were STUFFED armadillos that had escaped from the Smithsonian!? The real armadillo work (with leprosy) is still going on in Hawaii ('cause I got involved in that for a very short period of time in the Olde Days).? If you want an armadillo - ask someone that lives in Texas and I'm sure they'd be happy to round one up for you.? Watch them claws, though. ? Retirement... aaah...time to annoy people! ________________________________ From: "Rene J Buesa" To: "Shirley A. Powell" , "Jay Lundgren" , "Bob Richmond" Cc: histonet@lists.utsouthwestern.edu Sent: Monday, June 11, 2012 11:02:45 AM Subject: Re: [Histonet] Armadillos living at AFIP What happened to the armadillos in AFIP? Who knows what happened to them when AFIP CLOSED! Didn't you know that? Ren? J. ________________________________ From: Shirley A. Powell To: Jay Lundgren ; Bob Richmond Cc: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 11, 2012 12:54 PM Subject: RE: [Histonet] Re: best controls I want to know what happened to all the equipment there, especially the large Sartorius Microtome that was in the bone lab.? If anyone has that information please email me.? Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Monday, June 11, 2012 1:34 AM To: Bob Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: best controls I wonder what happened to all the leprous armadillos living at AFIP? ? ? ? ? ? ? ? Heeere little feller.... ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Mon Jun 11 18:01:01 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jun 11 18:01:06 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: References: <1339434165.66848.YahooMailNeo@web121405.mail.ne1.yahoo.com> <1668253677.963578.1339444397313.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: Of course I know AFIP closed, I was there the day they shut the doors. That doesn't answer the question of what happened to the armadillos. I'll bet someone put them in a box with a blanket and gave them away in front of Walmart. Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) From jaylundgren <@t> gmail.com Mon Jun 11 18:20:33 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jun 11 18:20:38 2012 Subject: [Histonet] giemsa stain control In-Reply-To: <1339429181.52681.YahooMailNeo@web121403.mail.ne1.yahoo.com> References: <1339429181.52681.YahooMailNeo@web121403.mail.ne1.yahoo.com> Message-ID: I'll bet you a nickel she means H. pylori controls. Good luck with that one Gale. I worked in a lab that is sitting on 100 lifetime's worth, but it's a secret....... (hint: inadequate sanitation, low social class, and crowded living conditions are related to H. pylori infection.) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From gayle.callis <@t> bresnan.net Mon Jun 11 19:04:14 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Jun 11 19:04:41 2012 Subject: [Histonet] Re:Glucose Oxidase peroxidase block that works for eosinophils Message-ID: <000001cd482e$e9011470$bb033d50$@bresnan.net> Dear Kim I attached the peroxidase block method via personal email which works for either frozen or FFPE sections. You must buy the correct, exact glucose from ICN as Sigma discontinued this. I included the original publication. You can also find this blocking method on the Vector website. This has been discussed on Histonet before but possibly not in the context of the original publication for eosinophils in frozen section in minimally fixed e.g. acetone. If the sections are FFPE, you may have to extend the time or stick to 60 minute incubation in the glucose oxidase/glucose mixture. We nicknamed this GLUOX Peroxidase Block. For frozens, we used 60 minutes incubation but later found a publication using 30 min blocking of endogenous peroxidase (ordinary and not eosinophils) in frozen sections. Timing is something you might play with to shorten the staining time. Theory is slow, continuous production of H2O2 removes/blocks peroxidase and pseudoperoxidases in tissues/cells. I had feedback that it worked on paraffin sections with stubborn endogenous peroxidase. Good luck Gayle Callis From techonebs <@t> comcast.net Mon Jun 11 19:17:27 2012 From: techonebs <@t> comcast.net (Matt Mincer) Date: Mon Jun 11 19:17:34 2012 Subject: [Histonet] regulating bodies Message-ID: <4FD68A97.10409@comcast.net> Hey All, Our version of the Histo Bath is on the market and ready to sell. No, I'm not trying to get a plug in for it. The manufacturer was asking me if there were any regulating bodies other than UL and CE etc. that they should register with. ex. CAP and JCAHO. Any thoughts on the matter would be greatly appreciated. Thanks Matt -- TECH ONE BIOMEDICAL SERVICES toll-free 866.497.3033 | office 708.383.6040 | cell 708.822.3738 From kelly_colpitts <@t> hotmail.com Mon Jun 11 20:57:28 2012 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Mon Jun 11 20:57:33 2012 Subject: [Histonet] HPV Control Tissue In-Reply-To: References: Message-ID: Hello Histonetters,We are looking for HPV positive control tissue for ISH testing for both high risk and low risk probes. Does anyone have a half dozen or so slides of each that they could spare? A block would be better but we'll take whatever you could spare. If you have something you could send us, please send me an email (kelly_colpitts@hotmail.com). Thanks,Kelly Colpitts, HT (ASCP)Nationwide Children's Hospital Columbus, OH From kelly_colpitts <@t> hotmail.com Mon Jun 11 21:01:04 2012 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Mon Jun 11 21:01:09 2012 Subject: [Histonet] Inventory Management System Message-ID: Hello Histonetters,Does anyone use inventory management software to keep track of their chemical inventories as well as other supplies? We are working on setting up Kanban cards but we are wondering what other labs are doing to help with inventory and ordering. Thanks for your input,Kelly Colpitts, HT (ASCP)Nationwide Childrens HospitalColumbus, OH From kelly_colpitts <@t> hotmail.com Mon Jun 11 21:09:42 2012 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Mon Jun 11 21:09:45 2012 Subject: [Histonet] Body Fluid Specimens Message-ID: Hi,Our current protocol for preparing cytospins from body fluids and cytospins is very limited. We basically add fixative and spin it down. This leaves us with pretty bloody slides and cell blocks at times. In my past experience, we did a lot more to the specimens and I think the blocks and slides looked better. Our hematology department prepares our CSF and brochoalveolar lavage slides for us. Does anyone have a protocol for specimen preparation they could email us? You can send me the protocol at kelly_colpitts@hotmail.com. Thanks so much,Kelly Colpitts, HT (ASCP)Nationwide Childrens HospitalColumbus, OH From nmhisto <@t> comcast.net Mon Jun 11 21:20:45 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Mon Jun 11 21:21:02 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: Message-ID: <744507110.982745.1339467645006.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Okay, now I have to know more.? I realize this is not strictly "histologically"-oriented, but since armadillos are used for research that would likely involve histology, I'd like to know more about the armadillos used at AFIP.? And I assume that by putting "them in a box with a blanket..." that these guys/gals were ah...living specimens when AFIP closed?? What was the research?? ----- Original Message ----- From: "Jay Lundgren" To: "Patsy Ruegg" Cc: nmhisto@comcast.net, "Rene J Buesa" , histonet@lists.utsouthwestern.edu, "Shirley A. Powell" , "Bob Richmond" Sent: Monday, June 11, 2012 5:01:01 PM Subject: Re: [Histonet] Armadillos living at AFIP Of course I know AFIP closed, I was there the day they shut the doors.? That doesn't answer the question of what happened to the armadillos.? I'll bet someone put them in a box with a blanket and gave them away in front of Walmart. ? ?????????????????????????????????????????????????????????Sincerely? ? ???????????????????????????????????????????????????????????????? Jay A. Lundgren, M.S., HTL (ASCP) ? ? ?????????????????????????????????????????????????????????????? From nmhisto <@t> comcast.net Mon Jun 11 21:23:37 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Mon Jun 11 21:23:41 2012 Subject: [Histonet] Armadillos Message-ID: <1596861012.982881.1339467817411.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Could this be something along the line of "The Armadillos of Walmart"?? Sorry - can't resist. From rsrichmond <@t> gmail.com Mon Jun 11 21:26:52 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Jun 11 21:26:55 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: <744507110.982745.1339467645006.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> References: <744507110.982745.1339467645006.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: Armadillos (Dasypus novemcinctus) are more or less unique in being easily infected with human leprosy; in fact, about 2% of Louisiana 'dillos are naturally infected. Obviously these animals would be quite an infection hazard. What I want to know is - is there any way to obtain armadillo tissue blocks as control material for staining Mycobacterium leprae? Bob Richmond Samurai Pathologist Knoxville TN On Mon, Jun 11, 2012 at 10:20 PM, wrote: > Okay, now I have to know more.? I realize this is not strictly > "histologically"-oriented, but since armadillos are used for research that > would likely involve histology, I'd like to know more about the armadillos > used at AFIP.? And I assume that by putting "them in a box with a > blanket..." that these guys/gals were ah...living specimens when AFIP > closed?? What was the research? > > ________________________________ > From: "Jay Lundgren" > To: "Patsy Ruegg" > Cc: nmhisto@comcast.net, "Rene J Buesa" , > histonet@lists.utsouthwestern.edu, "Shirley A. Powell" > , "Bob Richmond" > Sent: Monday, June 11, 2012 5:01:01 PM > > Subject: Re: [Histonet] Armadillos living at AFIP > > Of course I know AFIP closed, I was there the day they shut the doors.? That > doesn't answer the question of what happened to the armadillos.? I'll bet > someone put them in a box with a blanket and gave them away in front of > Walmart. > > ?????????????????????????????????????????????????????????Sincerely? > > ???????????????????????????????????????????????????????????????? Jay A. > Lundgren, M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > From mturner <@t> CSILaboratories.com Tue Jun 12 04:46:14 2012 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Tue Jun 12 04:46:27 2012 Subject: [Histonet] Armadillos living at AFIP In-Reply-To: References: <744507110.982745.1339467645006.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <467983BDAC5880438E2202F6B9BF79A8759107@exchange-be-01.CSI-LABS.local> Dr. Bob, I'd bet that 80 mph on a Louisiana highway after midnight might reap a few specimens.... Just bring a scalpel and a vat of formalin! Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: Bob Richmond [mailto:rsrichmond@gmail.com] Sent: Monday, June 11, 2012 10:27 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Armadillos living at AFIP Armadillos (Dasypus novemcinctus) are more or less unique in being easily infected with human leprosy; in fact, about 2% of Louisiana 'dillos are naturally infected. Obviously these animals would be quite an infection hazard. What I want to know is - is there any way to obtain armadillo tissue blocks as control material for staining Mycobacterium leprae? Bob Richmond Samurai Pathologist Knoxville TN On Mon, Jun 11, 2012 at 10:20 PM, wrote: > Okay, now I have to know more.? I realize this is not strictly > "histologically"-oriented, but since armadillos are used for research > that would likely involve histology, I'd like to know more about the > armadillos used at AFIP.? And I assume that by putting "them in a box > with a blanket..." that these guys/gals were ah...living specimens > when AFIP closed?? What was the research? > > ________________________________ > From: "Jay Lundgren" > To: "Patsy Ruegg" > Cc: nmhisto@comcast.net, "Rene J Buesa" , > histonet@lists.utsouthwestern.edu, "Shirley A. Powell" > , "Bob Richmond" > Sent: Monday, June 11, 2012 5:01:01 PM > > Subject: Re: [Histonet] Armadillos living at AFIP > > Of course I know AFIP closed, I was there the day they shut the doors.? > That doesn't answer the question of what happened to the armadillos.? > I'll bet someone put them in a box with a blanket and gave them away > in front of Walmart. > > ?????????????????????????????????????????????????????????Sincerely? > > ???????????????????????????????????????????????????????????????? Jay A. > Lundgren, M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Jun 12 07:37:52 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Jun 12 07:38:50 2012 Subject: [Histonet] Running Animal Tissue on Tissue Processor Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> We are a hospital diagnostic lab associated with a medical school. Generally we do not do however a lot of research tissue since the medical school does have a lab. However occasionally we are asked to process tissue and then cut H&E slides. In the past I have not seen a problem with this but wondered if anyone knew of any regulations or problems they have had that would prevent us from running this tissue. Thanks for your insight. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From talulahgosh <@t> gmail.com Tue Jun 12 08:20:38 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 12 08:20:43 2012 Subject: OT: Re: [Histonet] Armadillos Message-ID: I bet guy named Terry found a baby armadillo by the side of the road and nursed it. Now it sleeps under his bed. His name is Felix. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Mon, Jun 11, 2012 at 10:23 PM, wrote: > Could this be something along the line of "The Armadillos of Walmart"? > Sorry - can't resist. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Joyce.Cline <@t> meritushealth.com Tue Jun 12 08:39:54 2012 From: Joyce.Cline <@t> meritushealth.com (Joyce Cline) Date: Tue Jun 12 08:42:17 2012 Subject: [Histonet] Leitz 1512 micotome Message-ID: <4A187D87076BF348A9A9FBAD6A6659BCBA876ED3@MHXCHCM.wchsys.org> We have a 1512 microtome that can be used for parts or used to cut. All we ask is for shipping to be paid. Contact me off line. Joyce Cline, H. T. (ASCP) Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@meritushealth.com ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From settembr <@t> umdnj.edu Tue Jun 12 11:05:43 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Jun 12 11:06:01 2012 Subject: [Histonet] looking p16 - where is mtm labs? In-Reply-To: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> Message-ID: Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dhewitt <@t> hvhs.org Tue Jun 12 11:10:58 2012 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Tue Jun 12 11:11:27 2012 Subject: [Histonet] looking p16 - where is mtm labs? In-Reply-To: References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B016940D8@MX-HVB-02.hvhs.org> P16 is now only sold through Ventana, believe they bought MTM or the rights to P16. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, June 12, 2012 12:06 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: [Histonet] looking p16 - where is mtm labs? Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Jun 12 11:12:02 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jun 12 11:12:10 2012 Subject: [Histonet] looking p16 - where is mtm labs? In-Reply-To: References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> Message-ID: As far as I know you may only purchase MTM p16 through Ventana now...FDA approved. BD sells p16 buts it's RUO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, June 12, 2012 11:06 AM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: [Histonet] looking p16 - where is mtm labs? Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akelley1 <@t> slu.edu Tue Jun 12 11:13:08 2012 From: akelley1 <@t> slu.edu (Amanda Kelley) Date: Tue Jun 12 11:13:17 2012 Subject: [Histonet] looking p16 - where is mtm labs? In-Reply-To: References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> Message-ID: You have to get current pricing on P16 from your local Ventana rep. It is available both in the vial and in the Ventana dispenser. Amanda On Tue, Jun 12, 2012 at 11:05 AM, Settembre, Dana wrote: > Looking to get current pricing and availability from mtm labs on p16 and > no one > answers their phone - 800# nor their direct# > Seems Roche has taken them over. > Need to reach them. > Any help would be appreciated > Dana Settembre > Immunohistochemistry > University Hospital - UMDNJ > Newark, NJ > USA > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akelley1@slu.edu DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. From settembr <@t> umdnj.edu Tue Jun 12 11:46:10 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Jun 12 11:46:21 2012 Subject: [Histonet] THANKS for p16 info In-Reply-To: References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> Message-ID: From: Amanda Kelley [mailto:akelley1@slu.edu] Sent: Tuesday, June 12, 2012 12:13 PM To: Settembre, Dana Cc: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] looking p16 - where is mtm labs? You have to get current pricing on P16 from your local Ventana rep. It is available both in the vial and in the Ventana dispenser. Amanda On Tue, Jun 12, 2012 at 11:05 AM, Settembre, Dana > wrote: Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akelley1@slu.edu DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. From Joyce.Cline <@t> meritushealth.com Tue Jun 12 11:49:16 2012 From: Joyce.Cline <@t> meritushealth.com (Joyce Cline) Date: Tue Jun 12 11:54:22 2012 Subject: [Histonet] RE: Leitz 1512 micotome In-Reply-To: <4A187D87076BF348A9A9FBAD6A6659BCBA876ED3@MHXCHCM.wchsys.org> References: <4A187D87076BF348A9A9FBAD6A6659BCBA876ED3@MHXCHCM.wchsys.org> Message-ID: <4A187D87076BF348A9A9FBAD6A6659BCBA876ED4@MHXCHCM.wchsys.org> I am sorry to report that the 1512 microtome has multiple requests and I am closing the offer. Sorry, we didn't know they were still so popular. Joyce Cline, H. T. (ASCP) Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@meritushealth.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline [Joyce.Cline@meritushealth.com] Sent: Tuesday, June 12, 2012 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leitz 1512 micotome We have a 1512 microtome that can be used for parts or used to cut. All we ask is for shipping to be paid. Contact me off line. Joyce Cline, H. T. (ASCP) Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@meritushealth.com ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From akelley1 <@t> slu.edu Tue Jun 12 12:09:10 2012 From: akelley1 <@t> slu.edu (Amanda Kelley) Date: Tue Jun 12 12:09:21 2012 Subject: [Histonet] THANKS for p16 info In-Reply-To: References: <55C1FD599E1FFB479BAA2E4B5EF348C3013C53BEF61D@mmc-mail.ad.mhsil.com> Message-ID: You're Welcome! On Tue, Jun 12, 2012 at 11:46 AM, Settembre, Dana wrote: > ** ** > > ** ** > > *From:* Amanda Kelley [mailto:akelley1@slu.edu] > *Sent:* Tuesday, June 12, 2012 12:13 PM > *To:* Settembre, Dana > *Cc:* Vickroy, Jim; histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] looking p16 - where is mtm labs?**** > > ** ** > > You have to get current pricing on P16 from your local Ventana rep. It is > available both in the vial and in the Ventana dispenser. > Amanda**** > > On Tue, Jun 12, 2012 at 11:05 AM, Settembre, Dana > wrote:**** > > Looking to get current pricing and availability from mtm labs on p16 and > no one > answers their phone - 800# nor their direct# > Seems Roche has taken them over. > Need to reach them. > Any help would be appreciated > Dana Settembre > Immunohistochemistry > University Hospital - UMDNJ > Newark, NJ > USA > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet**** > > > > > -- > Amanda Kelley > Histology Supervisor > St. Louis University Medical School > Department of Pathology > 1402 S. Grand Blvd. > St. Louis Mo. 63104 > Phone: (314) 977-7868 > Fax: (314) 977-8740 > akelley1@slu.edu > > DISCLAIMER: The contents of this e-mail, including any attachments, > contain information which may be confidential, legally privileged, > proprietary in nature, or otherwise protected by law from disclosure, and > is solely for the use of the intended recipient(s). If you are not the > intended recipient, any use, disclosure or copying of this e-mail, > including any attachments, is unauthorized and strictly prohibited. If you > have received this e-mail in error, please notify us via return e-mail and > immediately delete all copies of it from your system. Any opinions either > expressed or implied in this e-mail and all attachments, are those of its > author only, and do not necessarily reflect those of Saint Louis University. > **** > -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akelley1@slu.edu DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. From dw18 <@t> uchicago.edu Tue Jun 12 12:11:25 2012 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Tue Jun 12 12:11:32 2012 Subject: [Histonet] Re: endogenous peroxidase Message-ID: <20120612121125.BLJ12222@mstore03.uchicago.edu> Hi Kim and Histonet Just a suggestion - I include sodium azide (to 0.01% final) in my antigen retrieval buffer. As we are repeatedly warned, azide is deadly to peroxidase activity, but this also includes endogenous peroxidases, so we can turn it to our advantage too. By the time I do my 'real' peroxidase staining, any residual free azide has long been washed away but continues to poison the endogenous enzyme. This has worked for me for neutrophils infiltrating brain injuries. Without using any other peroxidase quenching I get no background staining (including the neutrophils), but I can DAB-detect them just fine after azide if I use an anti-myeloperoxidase primary. -hope it works for you -David ============= Original Message: Histonet Digest, Vol 103, Issue 14 Message: 13 Date: Mon, 11 Jun 2012 09:37:13 -0700 (PDT) From: Kim Merriam Subject: [Histonet] endogenous peroxidase in eosinophils Anyone have tips for quenching endogenous peroxidase in eosinophils? Our standard px block is not doing the job (Biocare Peroxidazed-1). Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ================ David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 From Stacy.Giroux <@t> stjohn.org Tue Jun 12 12:44:11 2012 From: Stacy.Giroux <@t> stjohn.org (Giroux, Stacy) Date: Tue Jun 12 12:44:18 2012 Subject: [Histonet] Ventana U6 protocol Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3E0709CB7@AUSP03VMBX11.apptixhealth.net> Hi, I was wondering if anyone has a good protocol for running Ventana U6 DNP probe on the Benchmark XT or Ultra. Thanks, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From jaylundgren <@t> gmail.com Tue Jun 12 13:14:45 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jun 12 13:14:55 2012 Subject: [Histonet] ***News Flash*** Message-ID: BREAKING NEWS I. C. Critterz The Associated Press Washington, D.C.- Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings. The armadillos are allegedly covered in unusual skin lesions and missing several toes. Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland. While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. From lblazek <@t> digestivespecialists.com Tue Jun 12 13:19:17 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jun 12 13:20:13 2012 Subject: [Histonet] ***News Flash*** In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E3913E7C53834@IBMB7Exchange.digestivespecialists.com> Grab a scalpel! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, June 12, 2012 2:15 PM To: histonet Subject: [Histonet] ***News Flash*** BREAKING NEWS I. C. Critterz The Associated Press Washington, D.C.- Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings. The armadillos are allegedly covered in unusual skin lesions and missing several toes. Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland. While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELLE.LAMPHERE <@t> childrens.com Tue Jun 12 14:44:33 2012 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Tue Jun 12 14:43:49 2012 Subject: [Histonet] Histotech Grossing Protocols Message-ID: Is there anyone that would be willing to share their protocols with me concerning their high complexity qualified histotechs grossing surgical specimens? We are in the process of researching this endeavor and would like to get a feel for what other labs are doing and how they maintain compliance. Michelle Lamphere, HT (ASCP) Lead Tech, Histology Children's Medical Center 1935 Medical District Dr Dallas, TX 75235 tel 214.456.2318 michelle.lamphere@childrens.com Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
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From Nancy_Schmitt <@t> pa-ucl.com Tue Jun 12 10:35:28 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Jun 12 15:25:19 2012 Subject: [Histonet] processors Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Tue Jun 12 15:29:19 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 12 15:29:23 2012 Subject: [Histonet] processors In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> Message-ID: <1339532959.2363.YahooMailNeo@web121404.mail.ne1.yahoo.com> At this moment, just like in automobiles, all tissue processor can give you a good service and your selection should be based on your budget. Having said that, you ask for a comparison between 2, the Leica ASP300 vs. Sakura VIP6 and, again, price could decide but from the quality and durability VIP6 "wins hands down", at least for me! Ren? J. ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, June 12, 2012 11:35 AM Subject: [Histonet] processors Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA? 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Jun 12 15:54:20 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jun 12 15:54:26 2012 Subject: [Histonet] processors In-Reply-To: <1339532959.2363.YahooMailNeo@web121404.mail.ne1.yahoo.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> <1339532959.2363.YahooMailNeo@web121404.mail.ne1.yahoo.com> Message-ID: I would pick the VIP for its great performance and ease of use. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From nmhisto <@t> comcast.net Tue Jun 12 15:55:53 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Tue Jun 12 15:56:11 2012 Subject: [Histonet] processors In-Reply-To: <1339532959.2363.YahooMailNeo@web121404.mail.ne1.yahoo.com> Message-ID: <999514983.1019587.1339534553801.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> When I was evaluating the different processors in order to purchase a new one, I took all three manufacturers' brochures (Leica, Sakura and Thermo) and did a "spreadsheet" of the features.? I was raised on Sakura but the Leica won - hands down - and the Thermo didn't even place.? Service and reliability for the Leica beat the other two handily - and knowing that German engineering is a known quality was the kicker.? I'm not employed by Leica, but I played one on TV (huh?). ----- Original Message ----- From: "Rene J Buesa" To: "Nancy Schmitt" , histonet@lists.utsouthwestern.edu Sent: Tuesday, June 12, 2012 2:29:19 PM Subject: Re: [Histonet] processors At this moment, just like in automobiles, all tissue processor can give you a good service and your selection should be based on your budget. Having said that, you ask for a comparison between 2, the Leica ASP300 vs. Sakura VIP6 and, again, price could decide but from the quality and durability VIP6 "wins hands down", at least for me! Ren? J. ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, June 12, 2012 11:35 AM Subject: [Histonet] processors Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA? 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Tue Jun 12 16:03:28 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Jun 12 16:03:35 2012 Subject: [Histonet] processors In-Reply-To: <1339532959.2363.YahooMailNeo@web121404.mail.ne1.yahoo.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> <1339532959.2363.YahooMailNeo@web121404.mail.ne1.yahoo.com> Message-ID: I would have to agree with Rene. I have had two VIP's that were replaced after they were over 20 years old and they both were still running. I currently have 3 VIP 6's, thus far the only problem I've experienced was the fluid level sensor malfunctioned once. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 12, 2012 3:29 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] processors At this moment, just like in automobiles, all tissue processor can give you a good service and your selection should be based on your budget. Having said that, you ask for a comparison between 2, the Leica ASP300 vs. Sakura VIP6 and, again, price could decide but from the quality and durability VIP6 "wins hands down", at least for me! Ren? J. ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, June 12, 2012 11:35 AM Subject: [Histonet] processors Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA? 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From Allison_Scott <@t> hchd.tmc.edu Tue Jun 12 16:18:10 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Jun 12 16:18:17 2012 Subject: [Histonet] Varistain 24-4 Stainer Manual Message-ID: Hello to all in histoland. Does anyone have a maual for the Varistain 24-4 stainer? I have a friend looking for one. Any help in this will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From one_angel_secret <@t> yahoo.com Tue Jun 12 16:23:55 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jun 12 16:24:58 2012 Subject: [Histonet] ***News Flash*** In-Reply-To: References: Message-ID: <1339536235.42976.YahooMailNeo@web112320.mail.gq1.yahoo.com> Epidemic update******* ? Seems the Critters have been tracked back to south florida. Witnesses have described multiple critters on the sides of highways feet pointing up< small children assumed the critters had passed from a heart attack>. No injuries have been reported but one arrest was made. One man was caught surgically removing the brain from one such critter. Homeland security has been notified. Stay tuned for further details. ________________________________ From: Jay Lundgren To: histonet Sent: Tuesday, June 12, 2012 2:14 PM Subject: [Histonet] ***News Flash*** BREAKING NEWS I. C. Critterz The Associated Press ? ? Washington, D.C.-? Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings.? The armadillos are allegedly covered in unusual skin lesions and missing several toes.? Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland.? While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jun 12 16:30:04 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 12 16:30:04 2012 Subject: [Histonet] ***News Flash*** In-Reply-To: References: Message-ID: <002f01cd48e2$889ad670$99d08350$@rr.com> When I was at the AFIP and I had desk duty, I was afraid to walk through that 5th floor at night where all the animals were. I always wondered what they were doing up there. Now I know, adaptable armadillos with leprosy and ever increasing intelligence scouring D.C. I wonder if they are trained to attack politicians. Hmmmmmm. I said to my wife with leprosy "hey honey, what's eating you?" Joe the toe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, June 12, 2012 1:15 PM To: histonet Subject: [Histonet] ***News Flash*** BREAKING NEWS I. C. Critterz The Associated Press Washington, D.C.- Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings. The armadillos are allegedly covered in unusual skin lesions and missing several toes. Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland. While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jun 12 16:33:08 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 12 16:33:09 2012 Subject: [Histonet] ***News Flash*** In-Reply-To: <1339536235.42976.YahooMailNeo@web112320.mail.gq1.yahoo.com> References: <1339536235.42976.YahooMailNeo@web112320.mail.gq1.yahoo.com> Message-ID: <003001cd48e2$f6415d20$e2c41760$@rr.com> My question is "are they being found with empty Lonestar beer cans. Inside joke. Years ago in Texas, Lonestar Beer was running commercials with armadillos running across the street carrying Lonestar Beer. See, down here in Texas, armadillos are usually found as road kill. The commercials had them belly up holding a Lonestar Beer bottle. I used to have a stuffed one in my truck. Only in Texas!!! JTT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, June 12, 2012 4:24 PM To: Jay Lundgren; histonet Subject: Re: [Histonet] ***News Flash*** Epidemic update******* ? Seems the Critters have been tracked back to south florida. Witnesses have described multiple critters on the sides of highways feet pointing up< small children assumed the critters had passed from a heart attack>. No injuries have been reported but one arrest was made. One man was caught surgically removing the brain from one such critter. Homeland security has been notified. Stay tuned for further details. ________________________________ From: Jay Lundgren To: histonet Sent: Tuesday, June 12, 2012 2:14 PM Subject: [Histonet] ***News Flash*** BREAKING NEWS I. C. Critterz The Associated Press ? ? Washington, D.C.-? Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings.? The armadillos are allegedly covered in unusual skin lesions and missing several toes.? Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland.? While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge <@t> aol.com Tue Jun 12 17:34:52 2012 From: djemge <@t> aol.com (Djemge) Date: Tue Jun 12 18:05:48 2012 Subject: [Histonet] Re: Message-ID: <8CF1702B8200FCE-22DC-5897@webmail-d150.sysops.aol.com> ..I am back in control http://ferrari-blog.com/finance.twitter.php?vyqgoogleId=02p3 From CIngles <@t> uwhealth.org Tue Jun 12 18:20:17 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Jun 12 18:20:22 2012 Subject: [Histonet] ***News Flash*** OT References: <1339536235.42976.YahooMailNeo@web112320.mail.gq1.yahoo.com> <003001cd48e2$f6415d20$e2c41760$@rr.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A858@UWHC-MAIL2.uwhis.hosp.wisc.edu> Not to TRY to offend anyone who actually has Leprosy, but "Wierd Al" Yankovic has a song titled "Party at the Leper Colony". The puns are really groaners. Almost makes me wish I had the disease so my ears would fall off... :) I'll find my copy (yes I am a big "Wierd Al" fan) if anyone is actually interested in hearing it. He also has a song called "I Love my Pancreas" set to the beat of Beach Boys type music. There is another medical one but I can't recall it right now. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Tue 6/12/2012 4:33 PM To: 'Kim Donadio'; 'Jay Lundgren'; 'histonet' Subject: RE: [Histonet] ***News Flash*** My question is "are they being found with empty Lonestar beer cans. Inside joke. Years ago in Texas, Lonestar Beer was running commercials with armadillos running across the street carrying Lonestar Beer. See, down here in Texas, armadillos are usually found as road kill. The commercials had them belly up holding a Lonestar Beer bottle. I used to have a stuffed one in my truck. Only in Texas!!! JTT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, June 12, 2012 4:24 PM To: Jay Lundgren; histonet Subject: Re: [Histonet] ***News Flash*** Epidemic update******* Seems the Critters have been tracked back to south florida. Witnesses have described multiple critters on the sides of highways feet pointing up< small children assumed the critters had passed from a heart attack>. No injuries have been reported but one arrest was made. One man was caught surgically removing the brain from one such critter. Homeland security has been notified. Stay tuned for further details. ________________________________ From: Jay Lundgren To: histonet Sent: Tuesday, June 12, 2012 2:14 PM Subject: [Histonet] ***News Flash*** BREAKING NEWS I. C. Critterz The Associated Press Washington, D.C.- Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings. The armadillos are allegedly covered in unusual skin lesions and missing several toes. Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland. While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Jun 12 18:31:30 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jun 12 18:31:34 2012 Subject: [Histonet] ***News Flash*** In-Reply-To: <003001cd48e2$f6415d20$e2c41760$@rr.com> References: <1339536235.42976.YahooMailNeo@web112320.mail.gq1.yahoo.com> <003001cd48e2$f6415d20$e2c41760$@rr.com> Message-ID: <24FA9FB3-163F-4C4B-8604-8E76E86E912E@email.arizona.edu> I know some histonetters are groaning and wishing an end to this thread but I just have to add one more - some years ago I found an armadillo purse in a second hand shop here in Tucson. Had to rescue the poor critter and now it resides on a shelf among other "had to have" items in my living room. Joe, it didn't come with a beer can but I found a white rock with strange symbols on it inside. Maybe the critter was one of those escapees from AFIP with a secret code who met an untimely death. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From one_angel_secret <@t> yahoo.com Tue Jun 12 19:33:43 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jun 12 19:34:47 2012 Subject: [Histonet] ***News Flash*** In-Reply-To: <003001cd48e2$f6415d20$e2c41760$@rr.com> References: <1339536235.42976.YahooMailNeo@web112320.mail.gq1.yahoo.com> <003001cd48e2$f6415d20$e2c41760$@rr.com> Message-ID: <1339547623.79009.YahooMailNeo@web112313.mail.gq1.yahoo.com> Now that you mention it, I think they were all holding onto little white rocks that had a encryption on em. Except for one, that one had a bottle of gin and was wearing a bikini. The police took that one back to their office for a shake down. ________________________________ From: Joe Nocito To: 'Kim Donadio' ; 'Jay Lundgren' ; 'histonet' Sent: Tuesday, June 12, 2012 5:33 PM Subject: RE: [Histonet] ***News Flash*** My question is "are they being found with empty Lonestar beer cans. Inside joke. Years ago in Texas, Lonestar Beer was running commercials with armadillos running across the street carrying Lonestar Beer. See, down here in Texas, armadillos are usually found as road kill. The commercials had them belly up holding a Lonestar Beer bottle. I used to have a stuffed one in my truck. Only in Texas!!! JTT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, June 12, 2012 4:24 PM To: Jay Lundgren; histonet Subject: Re: [Histonet] ***News Flash*** Epidemic update******* ? Seems the Critters have been tracked back to south florida. Witnesses have described multiple critters on the sides of highways feet pointing up< small children assumed the critters had passed from a heart attack>. No injuries have been reported but one arrest was made. One man was caught surgically removing the brain from one such critter. Homeland security has been notified. Stay tuned for further details. ________________________________ From: Jay Lundgren To: histonet Sent: Tuesday, June 12, 2012 2:14 PM Subject: [Histonet] ***News Flash*** ? BREAKING NEWS I. C. Critterz The Associated Press ? ?? Washington, D.C.-? Residents of northern D.C., along with Bethesda, Silver Springs, and College Park are reporting a wave of armadillo sightings.? The armadillos are allegedly covered in unusual skin lesions and missing several toes.? Local zoologists are baffled, as armadillos are native to the south-central and southeastern United States and are not normally found in Maryland.? While no attacks have been reported, authorities are urging residents not to approach any armadillos they might encounter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Jun 12 19:39:23 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jun 12 19:40:13 2012 Subject: [Histonet] Histotech Grossing Protocols In-Reply-To: References: Message-ID: <1339547963.19112.YahooMailNeo@web112302.mail.gq1.yahoo.com> if your talking about compliance for the part where you need to have each type of specimen written out exactly how you do it. Basically, write what you do and do what you say. Grossing in of specimens is going to be different for every lab. The pathologist needs to be involved in this as they are the ones who ultimatly have to read it and diagnose off it. I hope Ive understood your question corrrectly? and have given some help. :) ________________________________ From: Michelle Lamphere To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, June 12, 2012 3:44 PM Subject: [Histonet] Histotech Grossing Protocols Is there anyone that would be willing to share their protocols with me concerning their high complexity qualified histotechs grossing surgical specimens?? We are in the process of researching this endeavor and would like to get a feel for what other labs are doing and how they maintain compliance. Michelle Lamphere, HT (ASCP) Lead Tech, Histology Children's Medical Center 1935 Medical District Dr Dallas, TX? 75235 tel 214.456.2318 michelle.lamphere@childrens.com Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ??? Please consider the environment before printing this e-mail
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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Jun 12 19:43:54 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jun 12 19:44:00 2012 Subject: [Histonet] Body Fluid Specimens In-Reply-To: References: Message-ID: <1339548234.47082.YahooMailNeo@web112307.mail.gq1.yahoo.com> Have you tried cytolyte? This will lyse the cells leaving only WBC and maybe a few red ones if its really bloody. How bloody it is will determin how many times you would add cytolyte , shake, then centrifuge at what ever your normal time is. Just a thought. ? Kim ________________________________ From: Kelly Colpitts To: histonet@lists.utsouthwestern.edu Sent: Monday, June 11, 2012 10:09 PM Subject: [Histonet] Body Fluid Specimens Hi,Our current protocol for preparing cytospins from body fluids and cytospins is very limited.? We basically add fixative and spin it down.? This leaves us with pretty bloody slides and cell blocks at times.? In my past experience, we did a lot more to the specimens and I think the blocks and slides looked better.? Our hematology department prepares our CSF and brochoalveolar lavage slides for us.? Does anyone have a protocol for specimen preparation they could email us?? You can send me the protocol at kelly_colpitts@hotmail.com. Thanks so much,Kelly Colpitts, HT (ASCP)Nationwide Childrens HospitalColumbus, OH ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dannyz89 <@t> yahoo.com Tue Jun 12 23:54:54 2012 From: dannyz89 <@t> yahoo.com (Danny Zapata) Date: Tue Jun 12 23:54:59 2012 Subject: [Histonet] Instruction manual Message-ID: <7107EA81-2952-48C5-9019-E6EE5F49A563@yahoo.com> Hi! I wasn't wondering if anyone has an instruction manual for a Shandon Varistain 24-3? The top part won't rotate properly Danny Zapata Dr.Norman Dermatology Office Tampa, Fl From dannyz89 <@t> yahoo.com Tue Jun 12 23:57:00 2012 From: dannyz89 <@t> yahoo.com (Danny Zapata) Date: Tue Jun 12 23:57:05 2012 Subject: [Histonet] Processing issue Message-ID: <4DCB5CCD-6191-49F0-96FD-A234AF71211D@yahoo.com> Would It be a grave problem if I run the tissue in a short run although it needed it to be placed in a routine regular run? Danny Zapata Dr. Norman Dermatology Office Tampa, Fl From ree3 <@t> leicester.ac.uk Wed Jun 13 03:54:00 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Jun 13 03:54:09 2012 Subject: [Histonet] cytology control slides? In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624639D653B9@TN001WEXMBX11.US.chs.net> References: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> <7EAFE982E328304DA6CE2B677BB7624639D653B9@TN001WEXMBX11.US.chs.net> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A6460A2B0D@EXC-MBX3.cfs.le.ac.uk> ???buccal.......... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: 11 June 2012 15:48 To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology control slides? We make a buckle smear and stain it. Quick and easy. The QC is for the pap stain quality. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, June 11, 2012 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytology control slides? ?Fellow techs, ? I was wondering what some of you do for a control slide ?for your cytology stainer. We will be staining urine cytology specifically. ? Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From TMcNemar <@t> lmhealth.org Wed Jun 13 04:52:17 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jun 13 04:52:17 2012 Subject: [Histonet] RE: processors In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E07ADE@PEITHA.wad.pa-ucl.com> Message-ID: VIP all the way. Still got one that I bought in 1985 as a backup.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, June 12, 2012 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processors Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Lindsay.Thorn <@t> osumc.edu Wed Jun 13 08:33:22 2012 From: Lindsay.Thorn <@t> osumc.edu (Thorn, Lindsay) Date: Wed Jun 13 08:33:38 2012 Subject: [Histonet] OSU Job Posting Message-ID: The Wexner Medical Center at The Ohio State University is looking for two (2) full time Histology Technicians/Technologists. The Ohio State is located in the heart of Columbus, Ohio. We have four beautiful seasons a year and enjoy a reasonable cost of living. Interested candidates should contact Lindsay Thorn at lindsay.thorn@osumc.edu or 614-293-5048 The histology laboratories are functional area labs within the division of Surgical Pathology; processes, embeds, sorts, cuts, files, and stains slides of tissue samples for diagnosis by surgical pathologists at The Ohio State University Medical Center; logs in specimens into the computer system, performs special stains, prepares solutions, and maintains general organizational paperwork; assists with training of students and staff; monitors equipment maintenance; responsible for record keeping of specified quality control and quality assurance. Education and Experience: Graduate of a school of Histology with an HT (ASCP) certification or equivalent preferred; HTL certification desired; Bachelors degree in biological sciences preferred; 2 or more years previous experience in a histology laboratory preferred; courses in college level biological sciences are not required, but desirable; experience in other areas of pathology a plus. Requires successful completion of a background check. From tkngflght <@t> yahoo.com Wed Jun 13 08:33:35 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jun 13 08:33:45 2012 Subject: [Histonet] HTL needed - FT Days--flexible hours Message-ID: <1339594415.25427.YahooMailNeo@web39405.mail.mud.yahoo.com> Small community hospital with a nice family feel (great schools/parks/cost of living) is seeking a registered HTL with a BS in one of the sciences.? The conversation is simple and you can talk with the facility folks without any committment...we're looking for the perfect fit for the hospital AND for you. ? My most favorite jobs have been small community hospitals...knowing you've made a difference for your pathologist and your patient doesn't get any better than in?small town hospitals. ? Give me a call--if this doesn't fit we'll work to find you a job that does! ? Cheryl ? ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From tkngflght <@t> yahoo.com Wed Jun 13 08:37:33 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jun 13 08:37:41 2012 Subject: [Histonet] Temp travelers needed Message-ID: <1339594653.25808.YahooMailNeo@web39402.mail.mud.yahoo.com> Hi Guys-- ? We're getting busy again and need a few more travelers.? ? ---If you're curious, we'll spend some time answering your questions.? ? ---If you're ready, we have a couple of short openings to talk about.? ? Either way the conversation is fun and informative--the good, the bad, the straight scoop--histotech to histotech!? ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From rjbuesa <@t> yahoo.com Wed Jun 13 09:05:45 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 13 09:05:53 2012 Subject: [Histonet] Processing issue In-Reply-To: <4DCB5CCD-6191-49F0-96FD-A234AF71211D@yahoo.com> References: <4DCB5CCD-6191-49F0-96FD-A234AF71211D@yahoo.com> Message-ID: <1339596345.22892.YahooMailNeo@web121406.mail.ne1.yahoo.com> That will depend on the tissue and the size/thickness of the specimens. Appreciate how they turned out and you will have the answer to your question. At least for me it is very difficult to answer your question without knowing the tissue characteristics. Ren? J. ________________________________ From: Danny Zapata To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, June 13, 2012 12:57 AM Subject: [Histonet] Processing issue Would It be a grave problem if I run the tissue in a short run although it needed it to be placed in a routine regular run? Danny Zapata Dr. Norman Dermatology Office Tampa, Fl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtighe <@t> trudeauinstitute.org Wed Jun 13 09:26:40 2012 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Wed Jun 13 09:26:55 2012 Subject: [Histonet] Antigen retrieval Message-ID: <108215434A378A4E8246C567E79DD48107E5BA20@CH1PRD0710MB356.namprd07.prod.outlook.com> Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike From liz <@t> premierlab.com Wed Jun 13 09:32:56 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 13 09:32:59 2012 Subject: [Histonet] RE: Antigen retrieval In-Reply-To: <108215434A378A4E8246C567E79DD48107E5BA20@CH1PRD0710MB356.namprd07.prod.outlook.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5F2E@SBS2K8.premierlab.local> Mike Retrieval methods are also based upon the antibody. During protocol development we try several different retrieval methods - no retrieval, enzymatic (proteinase K), pH6 and pH9, we then determine the best method and go from there. One retrieval method may not work for all antibodies. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Wednesday, June 13, 2012 8:27 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Antigen retrieval Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Wed Jun 13 10:13:19 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Jun 13 10:14:41 2012 Subject: [Histonet] RE: Antigen retrieval In-Reply-To: <108215434A378A4E8246C567E79DD48107E5BA20@CH1PRD0710MB356.namprd07.prod.outlook.com> References: <108215434A378A4E8246C567E79DD48107E5BA20@CH1PRD0710MB356.namprd07.prod.outlook.com> Message-ID: Mike, A good retrieval method will restore the epitope to a configuration that the antibody will recognize. The data sheet received with a commercial antibody may suggest a retrieval method to use, but if you are using a non-commercial source, trying a variety of methods may help unless the epitope is irreversibly altered by fixation. We run all new assays using pH 9, pH 6 and sometimes pH 3 [10 min treatment rather than 20 min] in addition to enzymatic retrieval using Pepsin, Trypsin or Protease and finally No Retrieval. Happy Hunting, Tresa _______________________________________________ Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MYang <@t> emc.org Wed Jun 13 10:39:30 2012 From: MYang <@t> emc.org (Yang, Mari) Date: Wed Jun 13 10:42:13 2012 Subject: [Histonet] cytology control slides? In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A6460A2B0D@EXC-MBX3.cfs.le.ac.uk> References: <50143451.1584341.1339418947643.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net><7EAFE982E328304DA6CE2B677BB7624639D653B9@TN001WEXMBX11.US.chs.net> <7722595275A4DD4FA225B92CDBF174A101A6460A2B0D@EXC-MBX3.cfs.le.ac.uk> Message-ID: <3340FC2AE9CFEE4E9D001D077700C6A2253EDA22@NT106.info.sys> I don't think there is a requirement for control slides for Cytology, only when IHCs and special stains are required. CAP requires a mechanism for monitoring cross-contamination. For urine cytology, I would insert a blank slide with your staining rack, if you are batch staining to ensure there are no floaters or cross contamination. Have the cytotech review the blank slide. The cytotech should also be monitoring the stain quality daily. Hope this helps. Thanks, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, June 13, 2012 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology control slides? ???buccal.......... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: 11 June 2012 15:48 To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology control slides? We make a buckle smear and stain it. Quick and easy. The QC is for the pap stain quality. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, June 11, 2012 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytology control slides? ?Fellow techs, ? I was wondering what some of you do for a control slide ?for your cytology stainer. We will be staining urine cytology specifically. ? Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From AHutton <@t> dh.org Wed Jun 13 10:51:27 2012 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Jun 13 10:54:03 2012 Subject: [Histonet] job opening Message-ID: <38A56C4F4630D348A50B3720409270870E0FE5E2@dhmail.dhorg.org> We currently have an open position for a HT, full time days. We are a community hospital located in southeastern PA. We average 11,000 cases per year, about half of which are GI biopsies. For more information or if interested, please visit www.dh.org From pruegg <@t> ihctech.net Wed Jun 13 11:35:45 2012 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Jun 13 11:35:58 2012 Subject: [Histonet] RE: Antigen retrieval Message-ID: <20120613093545.f86bd30e73b823f57b516b5451216a98.13a3ea5168.wbe@email01.secureserver.net> The most important part known positive tissue to use as everything you do is a shot in the dark protocol is not working or the control you are expressing that antigen. Each antibody has it's own requ pretreatment or not. We do what Liz does and sometimes ad buffer when nothing else works. Just to save on slides to start with no ar and ph6 and if we do not get good results, go to ed ta ph9, then PK, then ph8, if all these fail, we revert to overnight incuba buffers and/or enzy all of course a Some of my experien nuclei may prefer high ph noticed, definitely not something I h Regards,< Patsy -------- Original Message -------- Subject: [Histonet] RE: Antigen retrieval From: Elizabeth Chlipal To: 'Mike Tighe' <[2]mtighe@trudeauinstitute.org>, istonet@lists.utsouthwestern.edu)" <[5]histonet@lists.utsouthwestern.edu> Mike Retrieval methods are also based upon the antibody protocol development we try several different retrieval methods - retrieval, enzymatic (proteinase K), pH6 and pH9, we then determine the for all Liz Elizabeth A. Chlipala, BS, HTL(AS Manager Premier Laboratory, LLC PO Box 18592 (303) 682-3949 office (303) 682-9060 (303) 881-0763 cell [6]w Ship to address: 1567 Skywa Longmont, CO 80504 -----Original Message From: [7]histonet-bounces@lists.utsouthwestern.edu [[8]mailto:histonet-bounces@lists.utsouthw Mike Tighe Sent: Wednesday, June 13, 201 To: [9]hi ([10]histonet@lists.utsouthwestern.edu) Subject: [Histo Does anyone have a favorite antigen ret mouse tissues that they would be willing to share? I citrate buffer Ph6.0 with poor to moderate results. Thanks help! Mike ______________________ Histonet mailing list [11]Histonet@lists.utsouthwestern.edu [12]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet mailing l [13]Histonet@lis [14]http://lists.utsouthwestern.edu/mailman/listinfo References 1. 3D"mailto:liz@premierlab.com" 2. file://localhost/tmp/3D"m 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:histonet 6. 3D"http://www.premierlab.com"/ 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.e 8. 3D"mailto:histon 9. 3D"mailto:histonet@lists.utsouthwestern.edu" 10. 3D"mailto:histonet@lists.utsou 11. file://localhost/tmp/3D"mail 12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthwestern.edu/ From tyler.liebig <@t> thermofisher.com Wed Jun 13 11:57:02 2012 From: tyler.liebig <@t> thermofisher.com (Liebig, Tyler K.) Date: Wed Jun 13 11:53:52 2012 Subject: [Histonet] RE: Sequenza units for manual immunohistochemistry (Keri Colwell) In-Reply-To: <26FB002B7AEEA24D9D4998D9A3CF81AF14B0D625B8@USPHO-MXVS01.amer.thermo.com> References: <20120607141201.A4BEA1BB43B1@usmx02.thermofisher.com> <26FB002B7AEEA24D9D4998D9A3CF81AF14B0D625B8@USPHO-MXVS01.amer.thermo.com> Message-ID: <6746115DFA761840A605BB27624424AA18C7626CF6@USPHO-MXVS01.amer.thermo.com> Hello, This is Tyler Liebig with the Thermo Scientific IHC group. I'm responding to Keri's questions regarding the Sequenza manual staining. Keri you are correct that if the waste is not emptied and builds up too much in the bottom of the sequenza unit it will definitely stop the capillary action (Reagent can't flow both ways :-). However, this can be avoided by emptying the waste regularly between runs. You bring up a good question though that I didn't know the answer to: "How many slides can be stained before the waste hits the slides?" I did a quick calculation below that I hope is helpful. Total Waste Volume of Sequenza Rack: In a Sequenza rack the slides are held about 1.5 inches above the bottom of the tray. This allows about 270ml of waste before the level is close touching the slides. Reagent use per slide: If you stain a single slide with a long protocol then worst case scenario is about 7 steps. (H2O2 through Counterstain with a two step polymer) Volume of rinse buffer: This means you would rinse 8 times 2ml each (16ml total rinse buffer per slide worst case). For the staining reagents: The recommended volume is 0.1ml per reagent and 0.5ml for chromogen. So for 6 reagents plus chromogen that is 1.1ml and I will double it because I know some people add way more than needed. So 2.2ml per slide worst case. So to sum it up worst case scenario 18.2ml of waste is created per slide which means 14 slides (270/ml/18.2ml=14.8) can be stained before the waste risks touching the slides. The rack holds ten slides total. In the end I recommend users play is save and empty the waste after each batch of slides stained even though most user generate much less waste than the calculations above. I also included a link to a great description of Sequenza use on the Histonet posted by Gayle M Callis HTL/HT/MT (ASCP). I have referenced these details frequently myself (Thanks Gayle). http://www.histosearch.com/histonet/Jul02A/Longansweronusetechnicswi.html Hope that helps and if you have any other questions I'm happy to help offline (contact information is below). Thank you, Tyler Liebig Americas Product Manager - Immunohistochemistry Thermo Scientific Anatomical Pathology (510) 979-5000 ex 31816 (510) 299-1751 mobile Tyler.liebig@thermofisher.com From relia1 <@t> earthlink.net Wed Jun 13 12:06:55 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jun 13 12:25:03 2012 Subject: [Histonet] RELIA Hot Hot Hot Histology Job Alert!! Please take a look at some exciting opportunities. Message-ID: <005801cd4986$f04b1470$d0e13d50$@earthlink.net> Hello Histonetters! I hope everyone is having a great day and keeping an eye out for armadillos!! :-) I have some new positions that I am excited to share. I am excited about these opportunities because some are with brand new labs, some are exclusive to RELIA and some are just really really good positions. Here is the list: HT/HTL - Philadelphia, PA Mohs Tech - Dallas Mohs Tech - West Virginia Lead histotech - Columbus, OH IHC Specialist - Long Island Electron Microscopy Specialist - Long Island Night Shift Histotech - Lafayette, LA Molecular Technologist - Nashville, TN Histology Supervisor - Aurora, IL Histology Supervisor - Boston, MA If you or anyone you know might be interested in information on any of these positions please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. You could have an exciting new position sooner than you think OR a nice referral fee for spending cash for your summer vacation! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mdpraet <@t> gmail.com Wed Jun 13 12:28:37 2012 From: mdpraet <@t> gmail.com (mequita praet) Date: Wed Jun 13 12:28:41 2012 Subject: [Histonet] Re: writing Manuals Message-ID: Hi Madeleine Huey, I saw your post on histonet and am wondering if you have gotten someone to write your manuals. I have done that ensuring CLIA compliance. The last project I worked on, the doctor wanted them to be CAP compliant and I worked through the CAP checklist. However, they have not been through the inspection yet so, I don't know if everything was just right or not. I could certainly do more research. I do have my own consulting company - Histology Pro Consulting, LLC. If you haven't found anyone and would like to talk to me about this opportunity - let's set up a time to talk. Just let me know when it is a good time for me to call you. Here is my LinkedIn profile for you to look at. http://www.linkedin.com/pub/mequita-praet/22/302/91a Thank you for your time, Mequita Praet, HTL(ASCP)SLS From Lynn.Burton <@t> Illinois.gov Wed Jun 13 13:26:32 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jun 13 13:26:58 2012 Subject: [Histonet] RE: Varistain 24-4 Stainer Manual In-Reply-To: References: Message-ID: <4A6E2CACA1E017408EBA1B9911952CC006505C5D88@IL084EXMBX214.illinois.gov> I have an operator guide. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [Allison_Scott@hchd.tmc.edu] Sent: Tuesday, June 12, 2012 4:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Varistain 24-4 Stainer Manual Hello to all in histoland. Does anyone have a maual for the Varistain 24-4 stainer? I have a friend looking for one. Any help in this will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abdullahthayyil <@t> gmail.com Wed Jun 13 15:22:43 2012 From: abdullahthayyil <@t> gmail.com (Abdullah Thayyil) Date: Wed Jun 13 15:22:48 2012 Subject: [Histonet] TEM used needed Message-ID: Hi I am looking to start a TEM lab in India. I heard that used TEMs are available if one is willing to pay for shipment. Can someone give some information? Thanks Abdullah From mw <@t> personifysearch.com Wed Jun 13 15:23:27 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Jun 13 15:23:34 2012 Subject: [Histonet] Histology Opportunity Northeast (NJ/PA/NY/MA) Message-ID: <0b35a274d9ed95b848abc846bf66b5fc@mail.gmail.com> Good Afternoon Everyone! We are searching for a histologist who is looking for an exciting career with a world leader in manufacturing histology products. Our client is looking for a Applications Support Specialist to be the Histology expert in the Northeast to provide customer and sales support, and troubleshooting. This position offers competitive base salary + bonus and full benefits. The best part about this job is it allows someone the opportunity to join a growing company that offers endless career growth. If this sounds like something you would be interested in please send me your resume and we can set a time to talk in more detail. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From TMcNemar <@t> lmhealth.org Thu Jun 14 04:58:48 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 14 04:58:47 2012 Subject: [Histonet] Hologic Imaging System Message-ID: Hello all. For anyone who is using the Hologic Imaging System for Cytology.... I was wondering about the pre-analytical steps involved. I would appreciate any thoughts you could share regarding the process of preparation and scanning. Equipment, space requirements, time, labeling/barcoding, etc. Thanks so much! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From dannyz89 <@t> yahoo.com Thu Jun 14 05:32:36 2012 From: dannyz89 <@t> yahoo.com (Danny Zapata) Date: Thu Jun 14 05:32:43 2012 Subject: [Histonet] Derm lab Message-ID: <0AB9BA6F-5460-4A2B-AD5D-79D1BEF5C87A@yahoo.com> Hi everyone! I was wondering if anyone knows the typical protocol for skin that ask for a PAS staining procedure on the requisition form. Thanks! Hope everyone has a nice day! Danny Zapata Dr.Norman Dermatology Tampa, Fl Sent from my iPad From Keri.Colwell <@t> inspection.gc.ca Thu Jun 14 09:28:55 2012 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Thu Jun 14 09:29:04 2012 Subject: [Histonet] Thanks for the thoughts on Sequenza units! References: <4FD99FF1020000BF00008E2A@inspection.gc.ca> <4FD9A081020000BF00008E2C@inspection.gc.ca> <4FD9A0C7020000BF00008E30@inspection.gc.ca> Message-ID: <4FD9A0C6.C0AD.00BF.0@inspection.gc.ca> Hello All, I have compiled all of the responses to date regarding the question I posted on histonet about too much volume and reverse capillary action: (I've changed font styles to differentiate between responses) I have used them for quite some time. I was having problems with uneven staining and determined that it was from the being too full and was drawing up liquid from the bottom. There is a small opening on the end and I just empty the solution from it. I usually empty it after rinsing the antibody off and then usually before putting DAB or DAPI on depending on the number of slides stained. As for washing, I just wash in a solution of soapy water and then rinse in distilled. I use the coverplates for several staining times. I love the sequenza and prefer it to having an automatic stainer. I still use these and have 9 racks. The coverplates can be found on eBay, but I clean them in soapy bleach water, rinse well, and dry until the plastic gets brittle and they break. I use AEC 99% of the time so I do not generate the waste of DAB. If the racks are filing up with reagents and solutions, leave an empty slot and pipet it out. Otherwise, I dump it and and rinse after the run. For the antibodies and detection solutions, you only need 3-4 drops. I only fill the well to the top with rinses. We use these units, I have never had an issue with the waste reagents touching the bottom of the plates. We never use more than 3 drops of kit items, the wells are filled with buffer. It never seems to be alot of waste. After we finish staining we just rinse the holders out with running water for a few minutes and let air dry. We empty them before they get to that stage, as this would be bound to affect the efficient working of the Coverplates. This is Tyler Liebig with the Thermo Scientific IHC group. I'm responding to Keri's questions regarding the Sequenza manual staining. Keri you are correct that if the waste is not emptied and builds up too much in the bottom of the sequenza unit it will definitely stop the capillary action (Reagent can't flow both ways :-). However, this can be avoided by emptying the waste regularly between runs. You bring up a good question though that I didn't know the answer to: "How many slides can be stained before the waste hits the slides?" I did a quick calculation below that I hope is helpful. Total Waste Volume of Sequenza Rack: In a Sequenza rack the slides are held about 1.5 inches above the bottom of the tray. This allows about 270ml of waste before the level is close touching the slides. Reagent use per slide: If you stain a single slide with a long protocol then worst case scenario is about 7 steps. (H2O2 through Counterstain with a two step polymer) Volume of rinse buffer: This means you would rinse 8 times 2ml each (16ml total rinse buffer per slide worst case). For the staining reagents: The recommended volume is 0.1ml per reagent and 0.5ml for chromogen. So for 6 reagents plus chromogen that is 1.1ml and I will double it because I know some people add way more than needed. So 2.2ml per slide worst case. So to sum it up worst case scenario 18.2ml of waste is created per slide which means 14 slides (270/ml/18.2ml=14.8) can be stained before the waste risks touching the slides. The rack holds ten slides total. In the end I recommend users play is save and empty the waste after each batch of slides stained even though most user generate much less waste than the calculations above. I also included a link to a great description of Sequenza use on the Histonet posted by Gayle M Callis HTL/HT/MT (ASCP). I have referenced these details frequently myself (Thanks Gayle). http://www.histosearch.com/histonet/Jul02A/Longansweronusetechnicswi.html I have also read through Gayle's post on Histonet, it is worth reading through for some more insight on the use of these units. Keri From trathborne <@t> somerset-healthcare.com Thu Jun 14 11:03:49 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jun 14 11:02:42 2012 Subject: [Histonet] Urate crystals Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FE54D6@SMCMAIL01.somerset-healthcare.com> We have a piece of bone that the pathologist would like to process for urate crystals. The procedure we have is De Galantha's, which calls for decalcification by nitric acid, which we don't have. What other decalcification process can be used? Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From ctorrence <@t> kmcpa.com Thu Jun 14 13:37:15 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Thu Jun 14 13:37:36 2012 Subject: [Histonet] Billing IHC on MOHS Message-ID: <000301cd4a5c$b8a09230$29e1b690$@com> I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorrence@kmcpa.com From Joyce.Weems <@t> emoryhealthcare.org Thu Jun 14 14:20:23 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jun 14 14:21:21 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <000301cd4a5c$b8a09230$29e1b690$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> Message-ID: If each location is identified as a separate specimen, you can bill per specimen, is the way I understand it. e.g. Received separately - 88342 x 4 3:00 margin - A 6:00 margin - B 9:00 margin - C 12:00 margin - D If one specimen is received and divided into separate cassettes - 88342 x 1 A1 - 3:00 margin A2 - 6:00 margin A3 - 9:00 margin A4 - 12:00 margin Best, $1,783.00 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Torrence Sent: Thursday, June 14, 2012 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing IHC on MOHS I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorrence@kmcpa.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From CIngles <@t> uwhealth.org Thu Jun 14 14:32:52 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Jun 14 14:32:57 2012 Subject: [Histonet] Billing IHC on MOHS References: <000301cd4a5c$b8a09230$29e1b690$@com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> Is this per "Specimen" or per surgical site. Mohs specimens are usually cut into smaller pieces and inked after the excision is removed. I would think this constitutes x# of blocks from the same specimen. Same as an excision that is breadloafed into separate sections. I don't think it matters who does the cutting, although when we send these to path for permenents they are logged in a separate specimens but only measured, never breadloafed. Is it more dependent on how it is received in the lab even though the end result is still the same? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thu 6/14/2012 2:20 PM To: 'Carol Torrence'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing IHC on MOHS If each location is identified as a separate specimen, you can bill per specimen, is the way I understand it. e.g. Received separately - 88342 x 4 3:00 margin - A 6:00 margin - B 9:00 margin - C 12:00 margin - D If one specimen is received and divided into separate cassettes - 88342 x 1 A1 - 3:00 margin A2 - 6:00 margin A3 - 9:00 margin A4 - 12:00 margin Best, $1,783.00 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Torrence Sent: Thursday, June 14, 2012 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing IHC on MOHS I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorrence@kmcpa.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Thu Jun 14 15:13:54 2012 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jun 14 15:14:03 2012 Subject: [Histonet] (no subject) Message-ID: <4FD9FFB2020000AC000121AC@nodcdmg2.no.trinity-health.org> Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From FUNKM <@t> mercyhealth.com Thu Jun 14 15:14:02 2012 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jun 14 15:14:09 2012 Subject: [Histonet] (no subject) Message-ID: <4FD9FFBA020000AC000121B1@nodcdmg2.no.trinity-health.org> Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From bhartologist <@t> gmail.com Thu Jun 14 17:03:16 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Thu Jun 14 17:03:21 2012 Subject: [Histonet] ASCP HT exam In-Reply-To: References: Message-ID: Hello there histonetters. Some of you may remember some previous posts I've put up in regards to employment and taking the HT exam. I sat for my exam on Monday and......drum roll please.... I PASSED!!!! :):):):) I'm on the move to the bay area specifically Berkeley, and will be there by Sunday. So any info on jobs in that area would be greatly appreciated!!! From tahseen <@t> brain.net.pk Fri Jun 15 00:29:54 2012 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Fri Jun 15 00:30:23 2012 Subject: [Histonet] Bleach the carbons pigments Message-ID: <29646.203.135.35.66.1339738194.squirrel@brain.net.pk> Hi All, One of our pathologists wants to bleach the carbons pigments from cytology smears.According to Bancroft (page 263) it may be confused with melanin deposition but treatment with bleaching agents will show carbon unaffected. Is there any procedure? Muhammad Tahseen Senior Supervisor Histopathology SKMCH&RC Lahore Pakistan From Barry.R.Rittman <@t> uth.tmc.edu Fri Jun 15 05:02:20 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Jun 15 05:04:22 2012 Subject: [Histonet] Bleach the carbons pigments In-Reply-To: <29646.203.135.35.66.1339738194.squirrel@brain.net.pk> References: <29646.203.135.35.66.1339738194.squirrel@brain.net.pk> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9179D520320@UTHCMS1.uthouston.edu> Muhammad I really cannot see what the problem is. As far as I know there are no procedures for bleaching carbon, but as you can bleach melanin this should be sufficient for your pathologist . Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk [tahseen@brain.net.pk] Sent: Friday, June 15, 2012 12:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bleach the carbons pigments Hi All, One of our pathologists wants to bleach the carbons pigments from cytology smears.According to Bancroft (page 263) it may be confused with melanin deposition but treatment with bleaching agents will show carbon unaffected. Is there any procedure? Muhammad Tahseen Senior Supervisor Histopathology SKMCH&RC Lahore Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SStephenson <@t> lifecell.com Fri Jun 15 09:01:33 2012 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Fri Jun 15 09:01:43 2012 Subject: [Histonet] ASCP HT exam In-Reply-To: References: Message-ID: C*O*N*G*R*A*T*U*L*A*T*I*O*N*S!! woohoo. Sheryl Stephenson | Histology Technician ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bharti Parihar Sent: Thursday, June 14, 2012 6:03 PM To: Histonet Archive Subject: [Histonet] ASCP HT exam Hello there histonetters. Some of you may remember some previous posts I've put up in regards to employment and taking the HT exam. I sat for my exam on Monday and......drum roll please.... I PASSED!!!! :):):):) I'm on the move to the bay area specifically Berkeley, and will be there by Sunday. So any info on jobs in that area would be greatly appreciated!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 15 09:17:24 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 15 09:18:35 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: I was hoping someone else would jump in here that knows more about Mohs. I'm not sure if they are handled differently than other specimens because we don't do them. It seems that whatever is removed for separate dx would be billed as separate specimens, but there's not a lot of logic to this system! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Ingles Claire [mailto:CIngles@uwhealth.org] Sent: Thursday, June 14, 2012 3:33 PM To: Weems, Joyce K.; Carol Torrence; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing IHC on MOHS Is this per "Specimen" or per surgical site. Mohs specimens are usually cut into smaller pieces and inked after the excision is removed. I would think this constitutes x# of blocks from the same specimen. Same as an excision that is breadloafed into separate sections. I don't think it matters who does the cutting, although when we send these to path for permenents they are logged in a separate specimens but only measured, never breadloafed. Is it more dependent on how it is received in the lab even though the end result is still the same? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thu 6/14/2012 2:20 PM To: 'Carol Torrence'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing IHC on MOHS If each location is identified as a separate specimen, you can bill per specimen, is the way I understand it. e.g. Received separately - 88342 x 4 3:00 margin - A 6:00 margin - B 9:00 margin - C 12:00 margin - D If one specimen is received and divided into separate cassettes - 88342 x 1 A1 - 3:00 margin A2 - 6:00 margin A3 - 9:00 margin A4 - 12:00 margin Best, $1,783.00 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Torrence Sent: Thursday, June 14, 2012 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing IHC on MOHS I have a question about billing IHC on MOHS. When I go through the coding rules..I can defend it either way... I think. Ha! If you are doing the same antibody on one site with 5 individual zones, taking 5 independently labeled slides and each zone requires evaluation before continuing surgery. Do you charge 88342 times 5 or just once. I understand it would be just once if this was a routine surgical specimen but this is a horse of a different color. For example. Even for frozen sections performed during surgery, additional margins can be charged as additional frozen sections. Thanks in advance! Carol M. Torrence, HT(ASCP)CM ctorrence@kmcpa.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Fri Jun 15 11:59:17 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Fri Jun 15 11:59:41 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <000e01cd4b18$33eab310$9bc01930$@com> Thanks. I agree. Maybe we will hear from some derm labs today. My doctor wants to play it safe and charge once but that sure doesn't come close to covering costs of staining 5 slides, all different sites of the same lesion. It sounds like we can all argue both sides of the fence. Carol M. Torrence, HT(ASCP)CM ctorrence@kmcpa.com From kblack <@t> digestivehlth.com Fri Jun 15 12:30:39 2012 From: kblack <@t> digestivehlth.com (Konni Black) Date: Fri Jun 15 12:30:44 2012 Subject: [Histonet] Part Time HT/HTL Opening in Puyallup, WA Message-ID: Hi All, There is a part-time opening for a registered HT or HTL in Puyallup, WA, beautiful Mt. Rainier country! Excellent salary and flexible hours. Minimum 3 years experience required. If you are interested in more information, please contact Konni Black kblack@digestivehlth.com. Thank you From ctorrence <@t> kmcpa.com Fri Jun 15 13:20:20 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Fri Jun 15 13:20:43 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <000301cd4b23$860d9cb0$9228d610$@com> By George I've got it! This is what I read from "Coder's Choice CPT Plus" 88314 is for IHC performed on frozen tissue.......when a nonroutine histochemical stain on frozen tissue during Mohs surgery is utilized, report 88314 with modifier 59. Report one unit of 88314 for each special stain on each frozen surgical pathology block. Carol M. Torrence, HT(ASCP)CM Director of Laboratory Services KMC Dermatology - Pathology 2921 SW Wanamaker Dr. Topeka, Kansas 66614-5334 785-273-2788 ext 328 fax 785-272-6185 ctorrence@kmcpa.com From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 15 13:55:47 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 15 13:56:27 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <000301cd4b23$860d9cb0$9228d610$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> Message-ID: See, I even forgot the CPT code was 314!! Yeah, you.. so glad you found that for all the Mohs folks.. Have a good weekend everybody... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Carol Torrence [mailto:ctorrence@kmcpa.com] Sent: Friday, June 15, 2012 2:20 PM To: Weems, Joyce K.; 'Ingles Claire '; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing IHC on MOHS By George I've got it! This is what I read from "Coder's Choice CPT Plus" 88314 is for IHC performed on frozen tissue.......when a nonroutine histochemical stain on frozen tissue during Mohs surgery is utilized, report 88314 with modifier 59. Report one unit of 88314 for each special stain on each frozen surgical pathology block. Carol M. Torrence, HT(ASCP)CM Director of Laboratory Services KMC Dermatology - Pathology 2921 SW Wanamaker Dr. Topeka, Kansas 66614-5334 785-273-2788 ext 328 fax 785-272-6185 ctorrence@kmcpa.com ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 15 13:56:53 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 15 13:58:10 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <000301cd4b23$860d9cb0$9228d610$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> Message-ID: Well, actually I was thinking immunos on the paraffin block from the frozen.. I guess you all do it on frozen tho!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Carol Torrence [mailto:ctorrence@kmcpa.com] Sent: Friday, June 15, 2012 2:20 PM To: Weems, Joyce K.; 'Ingles Claire '; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing IHC on MOHS By George I've got it! This is what I read from "Coder's Choice CPT Plus" 88314 is for IHC performed on frozen tissue.......when a nonroutine histochemical stain on frozen tissue during Mohs surgery is utilized, report 88314 with modifier 59. Report one unit of 88314 for each special stain on each frozen surgical pathology block. Carol M. Torrence, HT(ASCP)CM Director of Laboratory Services KMC Dermatology - Pathology 2921 SW Wanamaker Dr. Topeka, Kansas 66614-5334 785-273-2788 ext 328 fax 785-272-6185 ctorrence@kmcpa.com ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From abdullahthayyil <@t> gmail.com Fri Jun 15 16:17:45 2012 From: abdullahthayyil <@t> gmail.com (Abdullah Thayyil) Date: Fri Jun 15 16:17:49 2012 Subject: [Histonet] TEM clarification Message-ID: Hi I am looking to start a TEM lab in India. I heard that used TEMs are available if one is willing to pay for shipment. Can someone give some information? I want to clarify that I am curently located in the USA. I can pick it up in the USA/Canada. Thanks Abdullah From one_angel_secret <@t> yahoo.com Sat Jun 16 10:39:42 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Jun 16 10:39:55 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <000301cd4b23$860d9cb0$9228d610$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> Message-ID: <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> I'd be very careful with this. Histochemical even if it's on frozen section such as 88314 does not mean immunohistochemical which is 88342 to my understanding. To my understanding anytime you use a antibody it's a 88342 code. Now as far as being able to code for each margin say from 3-6 or 6-9 o'clock etc that seems like it would fall under the rules for each site to me kind of like we would charge a 88307 or 88309 for excision with margins instead of a 88305 which pays less. I personally recommend this not be left in the hands of google or even histonet. Good information comes from both but you might just want to put a call into American society of MOHs and ask. Because over billing is serious business and can get you in a lot of trouble. I only say this because usually in MOHs you'll get the whole specimen which your checking for margins. Then any piece after that would be a separate specimen example that would be part " B " which you could charge separate for. And since different docs do this differently making a call couldn't hurt then you could come and share what they say with us :) Sent from my iPhone On Jun 15, 2012, at 2:20 PM, "Carol Torrence" wrote: > By George I've got it! This is what I read from "Coder's Choice CPT Plus" > > 88314 is for IHC performed on frozen tissue.......when a nonroutine > histochemical stain on frozen tissue during Mohs surgery is utilized, report > 88314 with modifier 59. Report one unit of 88314 for each special stain on > each frozen surgical pathology block. > > Carol M. Torrence, HT(ASCP)CM > Director of Laboratory Services > KMC Dermatology - Pathology > 2921 SW Wanamaker Dr. > Topeka, Kansas 66614-5334 > 785-273-2788 ext 328 > fax 785-272-6185 > ctorrence@kmcpa.com > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From badzrosari <@t> yahoo.com Sat Jun 16 11:01:00 2012 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Sat Jun 16 11:01:05 2012 Subject: [Histonet] Frozen Sections Falling Off Slides Message-ID: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland... I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised? please.Maybe youre using the same tissue tek cryo 3... From rjbuesa <@t> yahoo.com Sat Jun 16 11:08:36 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jun 16 11:08:42 2012 Subject: [Histonet] Frozen Sections Falling Off Slides In-Reply-To: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> References: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> Message-ID: <1339862916.62270.YahooMailNeo@web121405.mail.ne1.yahoo.com> Although it should NOT be the cause, I always used my cryostat at -20 to -25?C Second I always warmed the slides (at least 30?C) before picking the sections. This requires having a well extended cryosection and to pick them up with a "smooth movement". You will not have any chance of "rearranging-extending" the section. As a fixative I used NBF for 30 seconds ? to regular or special staining. Ren? J. ________________________________ From: Bernadette del Rosario To: "Histonet@lists.utsouthwestern.edu" Sent: Saturday, June 16, 2012 12:01 PM Subject: [Histonet] Frozen Sections Falling Off Slides Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland... I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised? please.Maybe youre using the same tissue tek cryo 3... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Jun 16 11:12:52 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Jun 16 11:13:03 2012 Subject: [Histonet] Frozen Sections Falling Off Slides In-Reply-To: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> References: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> Message-ID: <220A2BFD-B017-4867-B7B5-9360A045E927@yahoo.com> Was your leica set that cold? Maybe you could try warming the slide for a couple seconds before putting in alcohol. As far a charged slides I am amazed with the slides Dako sells for their IHC. Sent from my iPhone On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario wrote: > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland... > I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised please.Maybe youre using the same tissue tek cryo 3... > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Sun Jun 17 14:34:16 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Jun 17 14:34:20 2012 Subject: [Histonet] Frozen Sections Falling Off Slides In-Reply-To: <220A2BFD-B017-4867-B7B5-9360A045E927@yahoo.com> References: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> <220A2BFD-B017-4867-B7B5-9360A045E927@yahoo.com> Message-ID: Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the slides are electrostatically charged to keep the sections from falling off. Whether that's a bunch of hooha or it's actually true, our sections don't fall off, even after in situs. I believe most scientific suppliers sell their own brand of superfrost plus slides if you don't want to buy from Fisher. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio wrote: > Was your leica set that cold? Maybe you could try warming the slide for a > couple seconds before putting in alcohol. As far a charged slides I am > amazed with the slides Dako sells for their IHC. > > Sent from my iPhone > > On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario > wrote: > > > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen > section Machine...But i dont understand why i got problems sections falling > off slides.I used different types of slides non adhesive,adhesive(poly l > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > etc..then after distilled water wash out...sections started to peel > off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of > tissue- fibroma and salivary gland... > > I used Leica cryostat previously but i never got these problems...Even i > used non adhesive but sections still on the slides..Can someone advised > please.Maybe youre using the same tissue tek cryo 3... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From badzrosari <@t> yahoo.com Mon Jun 18 08:38:57 2012 From: badzrosari <@t> yahoo.com (Badz Yahoo) Date: Mon Jun 18 08:39:34 2012 Subject: [Histonet] Frozen Sections Falling Off Slides In-Reply-To: References: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com> <220A2BFD-B017-4867-B7B5-9360A045E927@yahoo.com> Message-ID: Hello netters...have someone tried doin frozen in a tissue mistakenly immersed in formalin??not so long but the inside still fresh..m sure the slides will wash out..fresh always better.. On Jun 17, 2012, at 10:34 PM, Emily Sours wrote: > Wow you can run frozen sections through those machines? That's awesome. I > figured it wouldn't be gentle enough to process them. > I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the > slides are electrostatically charged to keep the sections from falling > off. Whether that's a bunch of hooha or it's actually true, our sections > don't fall off, even after in situs. I believe most scientific suppliers > sell their own brand of superfrost plus slides if you don't want to buy > from Fisher. > > Emily > > "You see a peanut, day's off to a good start; you witness some soil it's a > jamboree for Vince Noir." > --Howard Moon, in "Charlie", The Mighty Boosh > > > > On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio wrote: > >> Was your leica set that cold? Maybe you could try warming the slide for a >> couple seconds before putting in alcohol. As far a charged slides I am >> amazed with the slides Dako sells for their IHC. >> >> Sent from my iPhone >> >> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario >> wrote: >> >>> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen >> section Machine...But i dont understand why i got problems sections falling >> off slides.I used different types of slides non adhesive,adhesive(poly l >> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken >> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative >> etc..then after distilled water wash out...sections started to peel >> off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of >> tissue- fibroma and salivary gland... >>> I used Leica cryostat previously but i never got these problems...Even i >> used non adhesive but sections still on the slides..Can someone advised >> please.Maybe youre using the same tissue tek cryo 3... >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Mon Jun 18 10:55:09 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Jun 18 10:55:14 2012 Subject: [Histonet] Employee productivity Message-ID: Hello to all in histoland. How is employee productivity calculated. I have my techs fill out a workload tally sheet daily. My boss wants me to take the data and come up with a productivity chart or graph that says who is productive or not. I don't need a tally sheet to say who is working or not. Is any one doing this? I need help. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rjbuesa <@t> yahoo.com Mon Jun 18 11:01:37 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 18 11:01:44 2012 Subject: [Histonet] Employee productivity In-Reply-To: References: Message-ID: <1340035297.45993.YahooMailNeo@web121406.mail.ne1.yahoo.com> With the data you have from your employees, calculate the number of units per unit time you get for the different tasks. Feed your data on Excel and prepare totals per lab and per employee and make graphs that you can display and discuss with your boss and your employees. Ren? J. ________________________________ From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 18, 2012 11:55 AM Subject: [Histonet] Employee productivity Hello to all in histoland.? How is employee productivity calculated.? I have my techs fill out a workload tally sheet daily.? My boss wants me to take the data and come up with a productivity chart or graph that says who is productive or not.? I don't need a tally sheet to say who is working or not.? Is any one doing this?? I need help. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Mon Jun 18 11:02:50 2012 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jun 18 11:03:01 2012 Subject: [Histonet] Fungus , GMS Aspergillus Message-ID: I am looking for natural occurring Fungus, GMS Aspergillus to use as my fungus control tissue. Someone from Histonet gave me a block about 3 years ago. I've just used the last of it. Does anyone have any to share again? Thanks Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From tkngflght <@t> yahoo.com Mon Jun 18 13:41:35 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Jun 18 13:41:40 2012 Subject: [Histonet] Sweetheart Job Openings - two of 'em! Message-ID: <1340044895.9322.YahooMailNeo@web39404.mail.mud.yahoo.com> Hi Histonetters! ? We have a plethora of openings all over the country-- entry level through management,?and I wanted to share these two: ? Seeking registered, grossing qualified tech(s) for each of two different labs. Both are/have: ????Physician office lab ????Day Shift (flexible) ????GREAT PAY and benies ????All NEW top of the line equipment ????Small biopsy specimens ????GREAT Pathologists ???? These are in the?north central/great lakes?states?area and available immediately for the qualified tech.? We will need at least a rough resume to get things rolling.? ? If curious, give me a call and I'll spend some time with you!? We're a full-service company with resume, interview and compensation support including coaching after you're hired.?? Take 5 minutes--might make a huge difference in your quality of life!! ? Cheryl ???? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org?or sign up at http://www.fullstaff.org/?Please?include your name and specialty in the body of the email. From PIXLEYSK <@t> UCMAIL.UC.EDU Mon Jun 18 13:47:46 2012 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Mon Jun 18 13:48:29 2012 Subject: [Histonet] RE: Histonet Digest, Vol 103, Issue 22 In-Reply-To: <6f0ef83c-df92-43a9-9064-9546a948e9a4@UCMailFE1.ad.uc.edu> References: <6f0ef83c-df92-43a9-9064-9546a948e9a4@UCMailFE1.ad.uc.edu> Message-ID: Dear All: I am seeking advice from someone who has experience with fixing and paraffin embedding rat muscle tissue. We want to dissect out muscles, fix them, embed in paraffin and then do H&E and immunostaining. Our first attempts, which involved immersion fixation in 4% paraformaldehyde, resulted in either very poor fixation or the tissue got cooked in the tissue processor. There were parts of the muscle that were hardened and completely disrupted. We suspect poor fixation, perhaps due to incomplete penetration? However, if possible, we would like to avoid having to fix via perfusion. Are there any good tricks? Please email me off the listserv. Thanks, Sarah Pixley Sarah.pixley@uc.edu From tajibade <@t> echd.org Mon Jun 18 14:05:10 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Mon Jun 18 14:05:22 2012 Subject: [Histonet] Power Path Message-ID: Hello everyone, I will like to get some idea about the Power Path system, if anybody out there is using this system or had used it before, what are the advantages and disadvantages of this system. Thank you. Tunde CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From CIngles <@t> uwhealth.org Mon Jun 18 15:30:46 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon Jun 18 15:32:12 2012 Subject: [Histonet] Frozen Sections Falling Off Slides References: <1339862460.77597.YahooMailNeo@web130203.mail.mud.yahoo.com><220A2BFD-B017-4867-B7B5-9360A045E927@yahoo.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A85D@UWHC-MAIL2.uwhis.hosp.wisc.edu> Just remember - No formalin, no need for retrieval. In my experience that is the hardest step on tissue sections. It also shortens the procedure a bunch too. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Sours Sent: Sun 6/17/2012 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Falling Off Slides Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the slides are electrostatically charged to keep the sections from falling off. Whether that's a bunch of hooha or it's actually true, our sections don't fall off, even after in situs. I believe most scientific suppliers sell their own brand of superfrost plus slides if you don't want to buy from Fisher. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio wrote: > Was your leica set that cold? Maybe you could try warming the slide for a > couple seconds before putting in alcohol. As far a charged slides I am > amazed with the slides Dako sells for their IHC. > > Sent from my iPhone > > On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario > wrote: > > > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen > section Machine...But i dont understand why i got problems sections falling > off slides.I used different types of slides non adhesive,adhesive(poly l > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > etc..then after distilled water wash out...sections started to peel > off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of > tissue- fibroma and salivary gland... > > I used Leica cryostat previously but i never got these problems...Even i > used non adhesive but sections still on the slides..Can someone advised > please.Maybe youre using the same tissue tek cryo 3... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arlange <@t> medicine.nevada.edu Mon Jun 18 15:48:13 2012 From: arlange <@t> medicine.nevada.edu (Alicia R. Lange) Date: Mon Jun 18 15:48:19 2012 Subject: [Histonet] Mouse Fetus Processing Message-ID: <9809822F80B1414C90EB4438713C8968013CF417@MEDX.medicine.nevada.edu> Hello Histoland, I have been given a project to process some mice fetuses. The research group is particularly interested in studying the GI track of each fetus. They range in size from 20 x 20 x 10 mm to 10 x 10 x 5 mm. Does anyone have any suggestions for processing, microtomy, sectioning, staining etc for these with the focus on the GI track? For now we have decided to cut the larger ones in half and section through the smaller ones with the microtome. There are no ideas for special stains at this point. Thanks in advance for any input or suggestions!!!! Alicia Lange University of Nevada, Reno Pathology Department arlange@medicine.nevada.edu From ctorrence <@t> kmcpa.com Mon Jun 18 16:23:01 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Mon Jun 18 16:23:22 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> Message-ID: <002201cd4d98$8adfc160$a09f4420$@com> Thanks everyone. I quoted the description straight from the PMIC coding manual. It is for use with 17311-17315, 88302-88309, 33221, and 88332. I was hoping to get a discussion about Mohs mysteries. I have also found it in two other publications. Mohs Society referred me AAD and I am waiting for an email response. After reviewing the slides that were given to our lab for staining I discovered that all five slides were from a single block containing two margins on each slide. I charged 88314-59. Kim you bring up another often controversial subject...when to charge 88307 or 88309. I became a CPC in 2005 but have found that if "you don't use you lose it and if not....they will change the rules on you anyway". Ha! I appreciate all comment. Carol T -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Saturday, June 16, 2012 10:40 AM To: Carol Torrence Cc: Weems, Joyce K.; Ingles Claire; Subject: Re: [Histonet] Billing IHC on MOHS I'd be very careful with this. Histochemical even if it's on frozen section such as 88314 does not mean immunohistochemical which is 88342 to my understanding. To my understanding anytime you use a antibody it's a 88342 code. Now as far as being able to code for each margin say from 3-6 or 6-9 o'clock etc that seems like it would fall under the rules for each site to me kind of like we would charge a 88307 or 88309 for excision with margins instead of a 88305 which pays less. I personally recommend this not be left in the hands of google or even histonet. Good information comes from both but you might just want to put a call into American society of MOHs and ask. Because over billing is serious business and can get you in a lot of trouble. I only say this because usually in MOHs you'll get the whole specimen which your checking for margins. Then any piece after that would be a separate specimen example that would be part " B " which you could charge separate for. And since different docs do this differently making a call couldn't hurt then you could come and share what they say with us :) Sent from my iPhone On Jun 15, 2012, at 2:20 PM, "Carol Torrence" wrote: > By George I've got it! This is what I read from "Coder's Choice CPT Plus" > > 88314 is for IHC performed on frozen tissue.......when a nonroutine > histochemical stain on frozen tissue during Mohs surgery is utilized, > report > 88314 with modifier 59. Report one unit of 88314 for each special > stain on each frozen surgical pathology block. > > Carol M. Torrence, HT(ASCP)CM > Director of Laboratory Services > KMC Dermatology - Pathology > 2921 SW Wanamaker Dr. > Topeka, Kansas 66614-5334 > 785-273-2788 ext 328 > fax 785-272-6185 > ctorrence@kmcpa.com > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Jun 18 17:02:30 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Jun 18 17:02:34 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <002201cd4d98$8adfc160$a09f4420$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> Message-ID: <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> Carol, ?????????? I obvioulsy have a talent for getting involved in controversial subjects, I really got to stop that LOL ? I used to have a CPT manual but no longer,they are very handy. If you got it out of that it seems to me thats the golden rule. My concern for you was that they recently changed how many you could bill per site. We had great discussion on it here. As I am not familiar with 88314 and got little info when I tried to look it up, coder wanted 20 bucks from me to see it. I am aware that Mohs has some codes specifically for Mohs, but my understanding is that CPT's are universal and each antibody would be 88342. However if your cpt manual says specifically a Mohs fs + antibody is 88314 I can only imagine the reimbursment for the frozen is included? I cant imagine why they would have a seperate one. Also when I was looking at what little coder would let me see for 88314, the termonology caught my eye. When I think histochemical stain , I think stains like a iron or trichrome or something of that nature. Thats why I mentioned 88342 because its specifically for immunohistochemistry, each antibody. ? Well, I hope I havnt made this clear as mud. I have no perfect answer just my thoughts. I am eager to hear what AAD says so keep us up to date. ? Thanks ? Kim D ________________________________ From: Carol Torrence To: 'Kim Donadio' Cc: "'Weems, Joyce K.'" ; 'Ingles Claire' ; histonet@lists.utsouthwestern.edu Sent: Monday, June 18, 2012 5:23 PM Subject: RE: [Histonet] Billing IHC on MOHS Thanks everyone.? I quoted the description straight from the PMIC coding manual.? It is for use with 17311-17315, 88302-88309, 33221, and 88332.? I was hoping to get a discussion about Mohs mysteries.? I have also found it in two other publications. Mohs Society referred me AAD and I am waiting for an email response. After reviewing the slides that were given to our lab for staining I discovered that all five slides were from a single block containing two margins on each slide.? I charged 88314-59. Kim you bring up another often controversial subject...when to charge 88307 or 88309. I became a CPC in 2005 but have found that if "you don't use you lose it and if not....they will change the rules on you anyway".? Ha! I appreciate all comment. Carol T -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Saturday, June 16, 2012 10:40 AM To: Carol Torrence Cc: Weems, Joyce K.; Ingles Claire; Subject: Re: [Histonet] Billing IHC on MOHS I'd be very careful with this. Histochemical even if it's on frozen section such as 88314 does not mean immunohistochemical which is 88342 to my understanding. To my understanding anytime you use a antibody it's a 88342 code. Now as far as being able to code for each margin say from 3-6 or 6-9 o'clock etc that seems like it would fall under the rules for each site to me kind of like we would charge a 88307 or 88309 for excision with margins instead of a 88305 which pays less. I personally recommend this not be left in the hands of google or even histonet. Good information comes from both but you might just want to put a call into American society of MOHs and ask. Because over billing is serious business and can get you in a lot of trouble. I only say this because usually in MOHs you'll get the whole specimen which your checking for margins. Then any piece after that would be a separate specimen example that would be part " B " which you could charge separate for.? And since different docs do this differently making a call couldn't hurt then you could come and share what they say with us :) Sent from my iPhone On Jun 15, 2012, at 2:20 PM, "Carol Torrence" wrote: > By George I've got it! This is what I read from "Coder's Choice CPT Plus" > > 88314 is for IHC performed on frozen tissue.......when a nonroutine > histochemical stain on frozen tissue during Mohs surgery is utilized, > report > 88314 with modifier 59.? Report one unit of 88314 for each special > stain on each frozen surgical pathology block. > > Carol M. Torrence, HT(ASCP)CM > Director of Laboratory Services > KMC Dermatology - Pathology > 2921 SW Wanamaker Dr. > Topeka, Kansas 66614-5334 > 785-273-2788 ext 328 > fax 785-272-6185 > ctorrence@kmcpa.com > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Mon Jun 18 17:29:32 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Mon Jun 18 17:29:53 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> Message-ID: <003301cd4da1$d5872650$809572f0$@com> Kim, You are too funny! It must be controversial if only three of us are talking about it and lots of people are 'doing it'. ha! That is funny about the $20. or not. I was only familiar with 88342 also until I started reading in the MOHS section of said CPT book. As I read it again, and again, I think I indeed might be interpreting it wrong about being applied to "Immunos". We will see what AAD says about my ADD. Stay tunedJ Carol From geralddavydov <@t> gmail.com Mon Jun 18 18:48:25 2012 From: geralddavydov <@t> gmail.com (Gerald Davydov) Date: Mon Jun 18 18:48:31 2012 Subject: [Histonet] 1. Re: Frozen Sections Falling Off Slides Message-ID: Bernadette, Tray to transfer frozen tissue to 50% formalin and 50%alcohol solution. On Monday, June 18, 2012, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Frozen Sections Falling Off Slides (Emily Sours) > 2. Re: Frozen Sections Falling Off Slides (Badz Yahoo) > 3. Employee productivity (Scott, Allison D) > 4. Re: Employee productivity (Rene J Buesa) > 5. Fungus , GMS Aspergillus (Cheri Miller) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 17 Jun 2012 15:34:16 -0400 > From: Emily Sours > > Subject: Re: [Histonet] Frozen Sections Falling Off Slides > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset=UTF-8 > > Wow you can run frozen sections through those machines? That's awesome. I > figured it wouldn't be gentle enough to process them. > I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the > slides are electrostatically charged to keep the sections from falling > off. Whether that's a bunch of hooha or it's actually true, our sections > don't fall off, even after in situs. I believe most scientific suppliers > sell their own brand of superfrost plus slides if you don't want to buy > from Fisher. > > Emily > > "You see a peanut, day's off to a good start; you witness some soil it's a > jamboree for Vince Noir." > --Howard Moon, in "Charlie", The Mighty Boosh > > > > On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio > >wrote: > > > Was your leica set that cold? Maybe you could try warming the slide for a > > couple seconds before putting in alcohol. As far a charged slides I am > > amazed with the slides Dako sells for their IHC. > > > > Sent from my iPhone > > > > On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario < > badzrosari@yahoo.com > > > wrote: > > > > > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen > > section Machine...But i dont understand why i got problems sections > falling > > off slides.I used different types of slides non adhesive,adhesive(poly l > > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections > taken > > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > > etc..then after distilled water wash out...sections started to peel > > off...My machine setting- chamber is -30 C;cryochamber is -39 C..example > of > > tissue- fibroma and salivary gland... > > > I used Leica cryostat previously but i never got these problems...Even > i > > used non adhesive but sections still on the slides..Can someone advised > > please.Maybe youre using the same tissue tek cryo 3... > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Mon, 18 Jun 2012 16:38:57 +0300 > From: Badz Yahoo > > Subject: Re: [Histonet] Frozen Sections Falling Off Slides > To: Emily Sours > > Cc: "histonet@lists.utsouthwestern.edu " > > > Message-ID: > > > Content-Type: text/plain; charset=us-ascii > > Hello netters...have someone tried doin frozen in a tissue mistakenly > immersed in formalin??not so long but the inside still fresh..m sure the > slides will wash out..fresh always better.. > > On Jun 17, 2012, at 10:34 PM, Emily Sours > > wrote: > > > Wow you can run frozen sections through those machines? That's awesome. > I > > figured it wouldn't be gentle enough to process them. > > I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the > > slides are electrostatically charged to keep the sections from falling > > off. Whether that's a bunch of hooha or it's actually true, our sections > > don't fall off, even after in situs. I believe most scientific suppliers > > sell their own brand of superfrost plus slides if you don't want to buy > > from Fisher. > > > > Emily > > > > "You see a peanut, day's off to a good start; you witness some soil it's > a > > jamboree for Vince Noir." > > --Howard Moon, in "Charlie", The Mighty Boosh > > > > > > > > On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio < > one_angel_secret@yahoo.com >wrote: > > > >> Was your leica set that cold? Maybe you could try warming the slide for > a > >> couple seconds before putting in alcohol. As far a charged slides I am > >> amazed with the slides Dako sells for their IHC. > >> > >> Sent from my iPhone > >> > >> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario < > badzrosari@yahoo.com > > >> wrote: > >> > >>> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen > >> section Machine...But i dont understand why i got problems sections > falling > >> off slides.I used different types of slides non adhesive,adhesive(poly l > >> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections > taken > >> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > >> etc..then after distilled water wash out...sections started to peel > >> off...My machine setting- chamber is -30 C;cryochamber is -39 > C..example of > >> tissue- fibroma and salivary gland... > >>> I used Leica cryostat previously but i never got these problems...Even > i > >> used non adhesive but sections still on the slides..Can someone advised > >> please.Maybe youre using the same tissue tek cryo 3... > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Mon, 18 Jun 2012 15:55:09 +0000 > From: "Scott, Allison D" > > Subject: [Histonet] Employee productivity > To: "histonet@lists.utsouthwestern.edu " > > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello to all in histoland. How is employee productivity calculated. I > have my techs fill out a workload tally sheet daily. My boss wants me to > take the data and come up with a productivity chart or graph that says who > is productive or not. I don't need a tally sheet to say who is working or > not. Is any one doing this? I need help. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > > > ------------------------------ > > Message: 4 > Date: Mon, 18 Jun 2012 09:01:37 -0700 (PDT) > From: Rene J Buesa > > Subject: Re: [Histonet] Employee productivity > To: "Scott, Allison D" >, > "histonet@lists.utsouthwestern.edu " > > > Message-ID: > <1340035297.45993.YahooMailNeo@web121406.mail.ne1.yahoo.com > > > Content-Type: text/plain; charset=iso-8859-1 > > With the data you have from your employees, calculate the number of units > per unit time you get for the different tasks. > Feed your data on Excel and prepare totals per lab and per employee and > make graphs that you can display and discuss with your boss and your > employees. > Ren? J. > > > ________________________________ > From: "Scott, Allison D" > > To: "histonet@lists.utsouthwestern.edu " < > histonet@lists.utsouthwestern.edu > > Sent: Monday, June 18, 2012 11:55 AM > Subject: [Histonet] Employee productivity > > Hello to all in histoland. How is employee productivity calculated. I > have my techs fill out a workload tally sheet daily. My boss wants me to > take the data and come up with a productivity chart or graph that says who > is productive or not. I don't need a tally sheet to say who is working or > not. Is any one doing this? I need help. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 5 > Date: Mon, 18 Jun 2012 11:02:50 -0500 > From: Cheri Miller > > Subject: [Histonet] Fungus , GMS Aspergillus > To: histonet > > Cc: "histonet-bounces@lists.utsouthwestern.edu " > > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > I am looking for natural occurring Fungus, GMS Aspergillus to use as my > fungus control tissue. Someone from Histonet gave me a block about 3 years > ago. I've just used the last of it. Does anyone have any to share again? > Thanks Cheri > > Cheryl A. Miller HT(ASCP)cm > Histology Supervisor > Physicians Laboratory Services > 4840 F Street > Omaha, NE. 68127-0999 > 402 731 4145 ext. 554 > > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 103, Issue 22 > ***************************************** > From one_angel_secret <@t> yahoo.com Mon Jun 18 19:15:04 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Jun 18 19:15:09 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <003301cd4da1$d5872650$809572f0$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> Message-ID: Oh I'm hooked. I have got to know the answer now lol we shall conquer this problem with your help and then we will all know once and for all. :) Thank you Sent from my iPhone On Jun 18, 2012, at 6:29 PM, "Carol Torrence" wrote: > Kim, > > > > You are too funny! It must be controversial if only three of us are talking about it and lots of people are ?doing it?. ha! > > > > That is funny about the $20? or not. I was only familiar with 88342 also until I started reading in the MOHS section of said CPT book. As I read it again, and again, I think I indeed might be interpreting it wrong about being applied to ?Immunos?. We will see what AAD says about my ADD. Stay tunedJ > > Carol > > From naveedafahim <@t> yahoo.ca Mon Jun 18 20:08:59 2012 From: naveedafahim <@t> yahoo.ca (naveeda arshad) Date: Mon Jun 18 20:09:05 2012 Subject: [Histonet] Re: frozen slides Message-ID: <1340068139.72205.YahooMailClassic@web161704.mail.bf1.yahoo.com> yes you can use charged slides for frozen section they work best In our lab we always use these slides we never had problemnaveeda --- On Mon, 6/18/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 103, Issue 22 To: histonet@lists.utsouthwestern.edu Received: Monday, June 18, 2012, 9:07 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Frozen Sections Falling Off Slides (Emily Sours) ???2. Re: Frozen Sections Falling Off Slides (Badz Yahoo) ???3. Employee productivity (Scott, Allison D) ???4. Re: Employee productivity (Rene J Buesa) ???5. Fungus , GMS Aspergillus? (Cheri Miller) ---------------------------------------------------------------------- Message: 1 Date: Sun, 17 Jun 2012 15:34:16 -0400 From: Emily Sours Subject: Re: [Histonet] Frozen Sections Falling Off Slides To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Wow you can run frozen sections through those machines? That's awesome.? I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides.? Fisher claims the slides are electrostatically charged to keep the sections from falling off.? Whether that's a bunch of hooha or it's actually true, our sections don't fall off, even after in situs.? I believe most scientific suppliers sell their own brand of superfrost plus slides if you don't want to buy from Fisher. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio wrote: > Was your leica set that cold? Maybe you could try warming the slide for a > couple seconds before putting in alcohol. As far a charged slides I am > amazed with the slides Dako sells for their IHC. > > Sent from my iPhone > > On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario > wrote: > > > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen > section Machine...But i dont understand why i got problems sections falling > off slides.I used different types of slides non adhesive,adhesive(poly l > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > etc..then after distilled water wash out...sections started to peel > off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of > tissue- fibroma and salivary gland... > > I used Leica cryostat previously but i never got these problems...Even i > used non adhesive but sections still on the slides..Can someone advised >? please.Maybe youre using the same tissue tek cryo 3... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Mon, 18 Jun 2012 16:38:57 +0300 From: Badz Yahoo Subject: Re: [Histonet] Frozen Sections Falling Off Slides To: Emily Sours Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain;??? charset=us-ascii Hello netters...have someone tried doin frozen in a tissue mistakenly immersed in formalin??not so long but the inside still fresh..m sure the slides will wash out..fresh always better.. On Jun 17, 2012, at 10:34 PM, Emily Sours wrote: > Wow you can run frozen sections through those machines? That's awesome.? I > figured it wouldn't be gentle enough to process them. > I'm a fan of Fisher Scientific superfrost plus slides.? Fisher claims the > slides are electrostatically charged to keep the sections from falling > off.? Whether that's a bunch of hooha or it's actually true, our sections > don't fall off, even after in situs.? I believe most scientific suppliers > sell their own brand of superfrost plus slides if you don't want to buy > from Fisher. > > Emily > > "You see a peanut, day's off to a good start; you witness some soil it's a > jamboree for Vince Noir." > --Howard Moon, in "Charlie", The Mighty Boosh > > > > On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio wrote: > >> Was your leica set that cold? Maybe you could try warming the slide for a >> couple seconds before putting in alcohol. As far a charged slides I am >> amazed with the slides Dako sells for their IHC. >> >> Sent from my iPhone >> >> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario >> wrote: >> >>> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen >> section Machine...But i dont understand why i got problems sections falling >> off slides.I used different types of slides non adhesive,adhesive(poly l >> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken >> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative >> etc..then after distilled water wash out...sections started to peel >> off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of >> tissue- fibroma and salivary gland... >>> I used Leica cryostat previously but i never got these problems...Even i >> used non adhesive but sections still on the slides..Can someone advised >> please.Maybe youre using the same tissue tek cryo 3... >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 18 Jun 2012 15:55:09 +0000 From: "Scott, Allison D" Subject: [Histonet] Employee productivity To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" Hello to all in histoland.? How is employee productivity calculated.? I have my techs fill out a workload tally sheet daily.? My boss wants me to take the data and come up with a productivity chart or graph that says who is productive or not.? I don't need a tally sheet to say who is working or not.? Is any one doing this?? I need help. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 4 Date: Mon, 18 Jun 2012 09:01:37 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Employee productivity To: "Scott, Allison D" , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <1340035297.45993.YahooMailNeo@web121406.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 With the data you have from your employees, calculate the number of units per unit time you get for the different tasks. Feed your data on Excel and prepare totals per lab and per employee and make graphs that you can display and discuss with your boss and your employees. Ren? J. ________________________________ From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 18, 2012 11:55 AM Subject: [Histonet] Employee productivity Hello to all in histoland.? How is employee productivity calculated.? I have my techs fill out a workload tally sheet daily.? My boss wants me to take the data and come up with a productivity chart or graph that says who is productive or not.? I don't need a tally sheet to say who is working or not.? Is any one doing this?? I need help. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 18 Jun 2012 11:02:50 -0500 From: Cheri Miller Subject: [Histonet] Fungus , GMS Aspergillus To: histonet Cc: "histonet-bounces@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for natural occurring Fungus, GMS Aspergillus to use as my fungus control tissue. Someone from Histonet gave me a block about 3 years ago. I've just used the last of it. Does anyone have any to share again?? Thanks Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 103, Issue 22 ***************************************** From info <@t> morphisto.de Tue Jun 19 00:25:14 2012 From: info <@t> morphisto.de (Morphisto GmbH) Date: Tue Jun 19 00:25:29 2012 Subject: [Histonet] RE: Histonet Digest, Vol 103, Issue 22 In-Reply-To: References: <6f0ef83c-df92-43a9-9064-9546a948e9a4@UCMailFE1.ad.uc.edu> Message-ID: <2A8B9056-2751-486B-B65A-451C079569B6@morphisto.de> Dear Sarah, for muscle histology I would recommend Bouin's fixative which give a very good fixation and sectioning properties. If you have a Leica microtome try to use the water bath for sectioning, and also the CoolClamp. This gives the best results. best regards Michael Am 18.06.2012 um 20:47 schrieb Pixley, Sarah (pixleysk): > Dear All: > I am seeking advice from someone who has experience with fixing and paraffin embedding rat muscle tissue. We want to dissect out muscles, fix them, embed in paraffin and then do H&E and immunostaining. Our first attempts, which involved immersion fixation in 4% paraformaldehyde, resulted in either very poor fixation or the tissue got cooked in the tissue processor. There were parts of the muscle that were hardened and completely disrupted. We suspect poor fixation, perhaps due to incomplete penetration? However, if possible, we would like to avoid having to fix via perfusion. Are there any good tricks? > Please email me off the listserv. > Thanks, > Sarah Pixley > Sarah.pixley@uc.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 60 Telefax: 069 / 989 72 1100 E-Mail: info@morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From relia1 <@t> earthlink.net Tue Jun 19 09:15:04 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jun 19 09:15:20 2012 Subject: [Histonet] RELIA HOT Histology Job Alert 6-19-2012 Exciting job opportunities with top employers ready to move now!! Message-ID: <008a01cd4e25$f0f077a0$d2d166e0$@earthlink.net> Hi Histonetters! I hope everybody is having a great day. I have a few new jobs to share with you as the phone is ringing off the hook and the list of opportunities keeps growing and growing. All of these jobs are permanent full time positions with excellent pay and great benefits. The clients I am working with are growing labs and the rest of the crew at each of these labs can't wait to meet their new co-worker. If you are happy where you are please take a look anyway remember if I place someone you refer you will earn a referral fee. Can't we all use some extra fun money this summer? Here are the newest positions: HT/HTL - Portland, ME Mohs Tech - Irving, TX HT/HTL - Philadelphia Lead Histologist - Columbus, OH IHC Specialist - Long Island, NY (NYS license req.) I also have opportunities in IL, MA, KY and TN. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. *You have the histology expertise, use my recruiting expertise to find the right position for you! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From shanonpink <@t> gmail.com Tue Jun 19 12:08:15 2012 From: shanonpink <@t> gmail.com (Shanon Pink) Date: Tue Jun 19 12:08:22 2012 Subject: [Histonet] H&E Stain for Epon-Embedded Tissue Message-ID: Is it possible to perform an H&E stain on tissues embedded in Epon plastic and cut into 0.5 - 1.0 micron sections? I can find protocols for doing so with tissues embedded in methacrylate, but not in Epon. Would the protocol for methacrylate also work for Epon, and if not, why? I am a student working on a research project in an electron microscopy lab and am trying to find a way to H&E-stain thick sections, or find a stain that very closely approximates H&E. Thank you in advance for any help you can offer! Shanon Pink University of Tennessee Health Science Center Department of Clinical Laboratory Science Memphis, TN From dw18 <@t> uchicago.edu Tue Jun 19 12:36:20 2012 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Tue Jun 19 12:36:25 2012 Subject: [Histonet] Re: rat muscle fixation In-Reply-To: <201206191704.CPM31988@mx02.uchicago.edu> References: <201206191704.CPM31988@mx02.uchicago.edu> Message-ID: <20120619123620.BLR33608@mstore03.uchicago.edu> Hello Sarah and Histonet You get wonderful formalin fixation of all rat tissues by transcardial perfusion - and no blood cells. You don't say whether you need a specific muscle and if it needs to be from a specific rat. Before you get into the bother of perfusing (& IACUC approval) you might want to contact your local neurology or neurobiology researchers because this is the method of choice for the brain. They will have lots of muscle as a by-product - the induced rigidity of the forelimb muscles (impressive to see the zombie effect) is a standard marker of good perfusion. You could check out some of their muscle to see if it is of a suitable quality. I'd ask the veterinarians in your animal facility to suggest someone who is doing this regularly, since this does require institutional approval as a non-survival surgery under deep anesthesia. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 ================================================ ---- Original message ---- Histonet Digest, Vol 103, Issue 22 ***************************************** Message: 2 Date: Mon, 18 Jun 2012 14:47:46 -0400 From: "Pixley, Sarah (pixleysk)" Subject: [Histonet] RE: Histonet Digest, Vol 103, Issue 22 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear All: I am seeking advice from someone who has experience with fixing and paraffin embedding rat muscle tissue. We want to dissect out muscles, fix them, embed in paraffin and then do H&E and immunostaining. Our first attempts, which involved immersion fixation in 4% paraformaldehyde, resulted in either very poor fixation or the tissue got cooked in the tissue processor. There were parts of the muscle that were hardened and completely disrupted. We suspect poor fixation, perhaps due to incomplete penetration? However, if possible, we would like to avoid having to fix via perfusion. Are there any good tricks? Please email me off the listserv. Thanks, Sarah Pixley Sarah.pixley@uc.edu From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Jun 19 12:46:00 2012 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Jun 19 12:46:48 2012 Subject: [Histonet] RE: rat muscle fixation In-Reply-To: <20120619123620.BLR33608@mstore03.uchicago.edu> References: <201206191704.CPM31988@mx02.uchicago.edu> <20120619123620.BLR33608@mstore03.uchicago.edu> Message-ID: Thank you Dr. Wright! We have full IACUC approval for perfusion and for all our procedures, and we have done a lot of paraformaldehyde perfusion fixation. It is just that for this study, we would like to avoid it, since it might interfere with other aspects of the experiment. So, we were wondering if there were any methods of fixing muscles that might get around the need for perfusion. For example, some studies add calcium to paraformaldehyde fixative and I was wondering if that might help preserve muscle better. We are working with the gastrocnemius muscle from adult rats, so it is quite thick (~1 cm diameter). I know that it may not be possible to get good immersion fixation for this big a piece of tissue. But I thought I might ask! Also, we could cut slabs of the muscle and then fix. Thanks, Sarah From tracyklady <@t> gmail.com Tue Jun 19 12:49:18 2012 From: tracyklady <@t> gmail.com (Tracy Karpinski) Date: Tue Jun 19 12:49:24 2012 Subject: [Histonet] ASCP HT exam Message-ID: Congrats on passing! What can I expect when I take it? Was it horrible? Kaiser regional in Berkeley are always hiring, usually night shift. They are union. From ctorrence <@t> kmcpa.com Tue Jun 19 13:10:33 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Tue Jun 19 13:10:54 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> Message-ID: <002601cd4e46$d232e340$7698a9c0$@com> The following is the response I recived from a coding specialist at the American Academy of Dermatology. I am trying not to be concerned that the reference is 6 years old but I think it clears up what we thought to be true. 88342 for IHC 88314 other ?special stains? Here is the description for 88314 according to November 2006 cpt Assistant article, the companion piece to the AMA CPT Code Book. The work of processing and interpreting one routine stain is included in the procedure 17311 - 17315 . This stain is usually hematoxylin and eosin, or toluidine blue. If other special stains are necessary after one routine stain, then the code for special stains may be used (88314 ) as well as immunoperoxidase stains (88342 ) or decalcification procedures (88311 ). Special stains are not typically used and in most Mohs practices are of low frequency. Each stain is reported only once per block, not per slide or per layer (stage). AMA CPT definition of a Block: Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. From andrea.conard <@t> gmail.com Tue Jun 19 13:18:56 2012 From: andrea.conard <@t> gmail.com (andrea conard) Date: Tue Jun 19 13:19:05 2012 Subject: [Histonet] billing IHC on Mohs Message-ID: Mohs or not 88314 is for a special stain. They call this histo chemical because chemical reactions take place in the tissue. This is not IHC. If you want to bill for IHC on frozen sections you bill 88342. I just lloked this up in my trusty coding guide. Hope that helps or adds to the controversy as the case may me. Andrea Conard, HT(ASCP), QIHC Supervisor Anatomic Pathology AtlantiCare Reginonal Medical Center From one_angel_secret <@t> yahoo.com Tue Jun 19 20:15:49 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jun 19 20:15:53 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <002601cd4e46$d232e340$7698a9c0$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> <002601cd4e46$d232e340$7698a9c0$@com> Message-ID: <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> Great team work! Job well done and a absolute answer is given. ? Thank you ________________________________ From: Carol Torrence To: 'Kim Donadio' Cc: "'Weems, Joyce K.'" ; 'Ingles Claire' ; histonet@lists.utsouthwestern.edu Sent: Tuesday, June 19, 2012 2:10 PM Subject: RE: [Histonet] Billing IHC on MOHS The following is the response I recived from a coding specialist at the American Academy of Dermatology.? I am trying not to be concerned that the reference is 6 years old but I think it clears up what we thought to be true.? 88342 for IHC 88314 other ?special stains? Here is the description for 88314 according to November 2006 cpt Assistant article, the companion piece to the AMA CPT Code Book. The work of processing and interpreting one routine stain is included in the procedure 17311- 17315. This stain is usually hematoxylin and eosin, or toluidine blue. If other special stains are necessary after one routine stain, then the code for special stains may be used (88314) as well as immunoperoxidase stains (88342) or decalcification procedures (88311). Special stains are not typically used and in most Mohs practices are of low frequency. Each stain is reported only once per block, not per slide or per layer (stage). AMA CPT definition of a Block:Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. From daniela.bodemer <@t> mcri.edu.au Tue Jun 19 20:30:46 2012 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Tue Jun 19 20:30:53 2012 Subject: [Histonet] Macrophage stain Message-ID: <9DF797D618351549B984596F01A1FE1D026C4A58@murmx.mcri.edu.au> Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From chapcl <@t> yahoo.com Tue Jun 19 20:45:08 2012 From: chapcl <@t> yahoo.com (William Chappell) Date: Tue Jun 19 20:45:14 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> <002601cd4e46$d232e340$7698a9c0$@com> <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> Message-ID: Well, I don't know if that settles that. I haven't responded, because I have not worked for a Mohs dermatopahtologist who runs Immunos (I have worked at numerous Mohs laboratories), however, this explanation is contradictory. "Each stain is reported only once per block, not per slide or per layer (stage)." Yet the definition of a block, "Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections." Every stage represent a new block in which slides are cut. These two statements are contradictory and need clarification. Now, my own opinion (again I have talked with my dermatopathologist and billing specialist and they are as lost as we) is that by definition, Mohs is a frozen section diagnosis that must be made by the surgeon (i.e., for a Mohs to be a mohs the surgeon removing the tissue must diagnose the tissue -- look it up). Every section taken, at every stage is a separate block of the same case. In the event you can charge immunos per case, only one charge can be made. If it can be shown that immunos can be charged per block (per the definition below), every immuno on every block from every stage can be charged. Now for the practicality -- we always start questions like this because medicare sets standards for billing that other insurance companies then adopt. We should NEVER ask, "what can we charge for," but should always ask, "what work did we do that it is fair for a patient to pay for." Ignore what medicare and insurance companies say, bill clients for the work we perform and for the results they get. How much more raw cost is there in staining two Mohs blocks with the same immuno? Is it fair to charge a patient double the amount for MUCH less than twice the work? Will Chappell HTL(ASCP), QIHC On Jun 19, 2012, at 9:15 PM, Kim Donadio wrote: > Great team work! Job well done and a absolute answer is given. > > Thank you > > > ________________________________ > From: Carol Torrence > To: 'Kim Donadio' > Cc: "'Weems, Joyce K.'" ; 'Ingles Claire' ; histonet@lists.utsouthwestern.edu > Sent: Tuesday, June 19, 2012 2:10 PM > Subject: RE: [Histonet] Billing IHC on MOHS > > > The following is the response I recived from a coding specialist at the American Academy of Dermatology. I am trying not to be concerned that the reference is 6 years old but I think it clears up what we thought to be true. > 88342 for IHC > 88314 other ?special stains? > Here is the description for 88314 according to November 2006 cpt Assistant article, the companion piece to the AMA CPT Code Book. > The work of processing and interpreting one routine stain is included in the procedure 17311- 17315. This stain is usually hematoxylin and eosin, or toluidine blue. If other special stains are necessary after one routine stain, then the code for special stains may be used (88314) as well as immunoperoxidase stains (88342) or decalcification procedures (88311). Special stains are not typically used and in most Mohs practices are of low frequency. Each stain is reported only once per block, not per slide or per layer (stage). > AMA CPT definition of a Block:Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Jun 19 22:04:01 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jun 19 22:05:22 2012 Subject: [Histonet] RE: Macrophage stain In-Reply-To: <9DF797D618351549B984596F01A1FE1D026C4A58@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D026C4A58@murmx.mcri.edu.au> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC7FF0@SBS2K8.premierlab.local> Serotec has two antibodies that will work a F4/80 and CD68, both are rat anti-mouse. We have used them in the same application that you will be using (mouse aorta) with good success. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bodemer@mcri.edu.au] Sent: Tuesday, June 19, 2012 7:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Macrophage stain Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimulah <@t> gmail.com Tue Jun 19 23:17:46 2012 From: kimulah <@t> gmail.com (=?ISO-2022-JP?B?GyRCTFpCPE01PHkbKEI=?=) Date: Tue Jun 19 23:17:49 2012 Subject: [Histonet] RE: Macrophage stain In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC7FF0@SBS2K8.premierlab.local> References: <9DF797D618351549B984596F01A1FE1D026C4A58@murmx.mcri.edu.au> <14E2C6176416974295479C64A11CB9AE011390CC7FF0@SBS2K8.premierlab.local> Message-ID: Hi. all Let me add another question linked to marcophage staining. So far we have done macrophage staining several times with F4/80, and in most of the cases IHC images were quite beautiful, for liver, spleen, adipose tissue and so on. BUT, we have not succeeded in staining lung alveolar macrophages.... One of my friends(pathologist) told me that F4/80 was not good for alveolar macrophage but he also don't know how to deal with this problem. Does anyone know what is appropriate antibody for staining lung alveolar macrophage? Thanks in advance Hiro 2012/6/20 Elizabeth Chlipala : > Serotec has two antibodies that will work a F4/80 and CD68, both are rat anti-mouse. ?We have used them in the same application that you will be using (mouse aorta) with good success. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949?office > (303) 881-0763?cell > (303) 682-9060?fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bodemer@mcri.edu.au] > Sent: Tuesday, June 19, 2012 7:30 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Macrophage stain > > Hi all, > > > > I would like to stain for macrophages in mice aorta (5um frozen > sections). Would you have suggestions on which stains to use and perhaps > share the protocol? > > > > Thanks in advance, > > > > Daniela Bodemer > > Research Assistant > > Surgical Research, Infection and Immunity > > > > Murdoch Childrens Research Institute > > The Royal Children's Hospital > > Flemington Road Parkville Victoria 3052 Australia > > T 03 9936 6676 ? ? T (03 9936 6794) > > E daniela.bodemer@mcri.edu.au > > www.mcri.edu.au > > > > This e-mail and any attachments to it (the "Communication") are, unless > otherwise stated, confidential, may contain copyright material and is > for the use only of the intended recipient. If you receive the > Communication in error, please notify the sender immediately by return > e-mail, delete the Communication and the return e-mail, and do not read, > copy, retransmit or otherwise deal with it. Any views expressed in the > Communication are those of the individual sender only, unless expressly > stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 > 006 566 972 or any of its related entities. MCRI does not accept > liability in connection with the integrity of or errors in the > Communication, computer virus, data corruption, interference or delay > arising from or in respect of the Communication. > > P ? ? ?Please consider the environment before printing this email > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jun 20 03:22:13 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jun 20 03:22:21 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> <002601cd4e46$d232e340$7698a9c0$@com> <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> Message-ID: I'm trying to think this through. Like Will said, if it can be shown that you can charge immunos per block... I thought we could only charge IHC per specimen these days. Wouldn't each stage be a different specimen? I would think billing per block of the same stage would be over charging. Why would the logic change because this is MOHS? It is still the same pathology billing code after all (88342). I would also think that if this situation is coming up often that someone is having trouble reading the H&Es. Mark On Tuesday, June 19, 2012, William Chappell wrote: > Well, I don't know if that settles that. > > I haven't responded, because I have not worked for a Mohs > dermatopahtologist who runs Immunos (I have worked at numerous Mohs > laboratories), however, this explanation is contradictory. "Each stain is > reported only once per block, not per slide or per layer (stage)." Yet the > definition of a block, "Tissue flattened by cutting into pieces, embedded, > and frozen in mounting medium used by histotechnologists to embed tissue > for frozen sections." Every stage represent a new block in which slides > are cut. These two statements are contradictory and need clarification. > > Now, my own opinion (again I have talked with my dermatopathologist and > billing specialist and they are as lost as we) is that by definition, Mohs > is a frozen section diagnosis that must be made by the surgeon (i.e., for a > Mohs to be a mohs the surgeon removing the tissue must diagnose the tissue > -- look it up). Every section taken, at every stage is a separate block of > the same case. In the event you can charge immunos per case, only one > charge can be made. If it can be shown that immunos can be charged per > block (per the definition below), every immuno on every block from every > stage can be charged. > > Now for the practicality -- we always start questions like this because > medicare sets standards for billing that other insurance companies then > adopt. We should NEVER ask, "what can we charge for," but should always > ask, "what work did we do that it is fair for a patient to pay for." > Ignore what medicare and insurance companies say, bill clients for the > work we perform and for the results they get. How much more raw cost is > there in staining two Mohs blocks with the same immuno? Is it fair to > charge a patient double the amount for MUCH less than twice the work? > > Will Chappell HTL(ASCP), QIHC > > On Jun 19, 2012, at 9:15 PM, Kim Donadio wrote: > > > Great team work! Job well done and a absolute answer is given. > > > > Thank you > > > > > > ________________________________ > > From: Carol Torrence > > To: 'Kim Donadio' > > Cc: "'Weems, Joyce K.'" ; 'Ingles > Claire' ; histonet@lists.utsouthwestern.edu > > Sent: Tuesday, June 19, 2012 2:10 PM > > Subject: RE: [Histonet] Billing IHC on MOHS > > > > > > The following is the response I recived from a coding specialist at the > American Academy of Dermatology. I am trying not to be concerned that the > reference is 6 years old but I think it clears up what we thought to be > true. > > 88342 for IHC > > 88314 other ?special stains? > > Here is the description for 88314 according to November 2006 cpt > Assistant article, the companion piece to the AMA CPT Code Book. > > The work of processing and interpreting one routine stain is included in > the procedure 17311- 17315. This stain is usually hematoxylin and eosin, or > toluidine blue. If other special stains are necessary after one routine > stain, then the code for special stains may be used (88314) as well as > immunoperoxidase stains (88342) or decalcification procedures (88311). > Special stains are not typically used and in most Mohs practices are of low > frequency. Each stain is reported only once per block, not per slide or per > layer (stage). > > AMA CPT definition of a Block:Tissue flattened by cutting into pieces, > embedded, and frozen in mounting medium used by histotechnologists to embed > tissue for frozen sections. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Peter.Tree <@t> abdserotec.com Wed Jun 20 04:20:08 2012 From: Peter.Tree <@t> abdserotec.com (Peter Tree) Date: Wed Jun 20 04:20:23 2012 Subject: [Histonet] RE: Macrophage stain In-Reply-To: References: <9DF797D618351549B984596F01A1FE1D026C4A58@murmx.mcri.edu.au> <14E2C6176416974295479C64A11CB9AE011390CC7FF0@SBS2K8.premierlab.local> Message-ID: <2CCADC88BBCAEF4A9BA84CDA42EE5286096FC2@SRV-MUC-105.morphosys.com> Hiro, You are correct, alveolar macrophages do express less F4/80 than other tissue macrophages. I refer you to, for example: Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine macrophages. Curr Protoc Immunol. 2008 Nov;Chapter 14:Unit 14.1. PubMed PMID: 19016445; PubMed Central PMCID: PMC2834554. Here they state: Alveolar macrophages are functionally different from peritoneal macrophages. Peritoneal macrophages are F4/80high, CD11bhigh, and CD68+. Bone marrow-derived macrophages also express F4/80high, CD11bhigh, and CD68+. However, alveolar macrophages display a F4/80low, CD11blow, and CD68+ phenotype under normal physiological conditions. Staining alveolar macrophages with CD68 (clone FA-11) would be a practical alternative for cryostat material, it just looks less pretty. Good luck. Peter Tree Technical Data Manager AbD Serotec Morphosys UK Ltd Endeavour house Langford Lane Kidlington Oxon OX5 1GE e.mail: peter.tree@abdserotec.com ________________________________ MorphoSys UK Ltd., Registered in England No: 1604642 Registered office: Greyfriars Court, Paradise Square, Oxford OX1 1BB, UK ________________________________ This message may contain confidential and/or privileged information. If you are not the addressee or authorized to receive this for the addressee, you must not use, copy, disclose or take any action based on this message or any information herein. If you have received this message in error, please advise the sender immediately by reply e-mail and delete this message. Thank you for your cooperation. Diese E-Mail kann vertrauliche und/oder rechtlich geschuetzte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtuemlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet. From mmooreht <@t> yahoo.com Wed Jun 20 06:03:36 2012 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Wed Jun 20 06:03:43 2012 Subject: [Histonet] (no subject) Message-ID: <1340190216.83667.androidMobile@web161502.mail.bf1.yahoo.com>

http://89.249.89.87/academicdiagram/Timothy_Davies34/

From one_angel_secret <@t> yahoo.com Wed Jun 20 06:15:58 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Jun 20 06:16:06 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> <002601cd4e46$d232e340$7698a9c0$@com> <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> Message-ID: <1340190958.44522.YahooMailNeo@web112302.mail.gq1.yahoo.com> The terminology is confusing< stage and per layer are not the same thing to me, depends on how you think about it >. Seems that Moh's and yes I have done this, can be done different. You have the Dr Mohs way of getting the what we call Donut specimen, which gets inked, nicked, embedded flat then frozen. This is your 1st stage. or block "A" if you will. Now some people will cut this into 2 pieces and even more and this creates more blocks. All of that first specimen is still the 1st stage and considered block A. You bill for block A, not for A1, A2 etc. and yes you are correct each additional stage( had to go back because 1st stage was positive)?would be billiable again, same senario above. The second stage Block "B" billiable for "B" only, not B1, B2 etc. This was something we had much discussion about here as they recently changed multiple billing on the same site, or for Mohs you would call it Same Stage. This is my interpretation. ? and to make sure I am not mis-interpreting you, you're not considering every section as you mentioned a seperate stage? While I can see that?Carol's post has (stage) next to per layer, I have to wonder?if this is?exactly out of the book? Because I would disagree with the two terms meaning the same as well.? Refer to above comment for my definition of Stage and hopfully it has clarity. :) ? As far as ignoring Medicare, I would never suggest that. And for ignoring insurance companies, well you can try all they can do is deny payment and then you get to haggle with them. ? I'm not sure I can add anything else to this conversation so I will let the rest have at it for now. ? Hope everyone has a fantastic week! ? Kim D ? ? ________________________________ From: William Chappell To: Kim Donadio Cc: Carol Torrence ; "histonet@lists.utsouthwestern.edu" ; 'Ingles Claire' Sent: Tuesday, June 19, 2012 9:45 PM Subject: Re: [Histonet] Billing IHC on MOHS Well, I don't know if that settles that. I haven't responded, because I have not worked for a Mohs dermatopahtologist who runs Immunos (I have worked at numerous Mohs laboratories), however, this explanation is contradictory.? "Each stain is reported only once per block, not per slide or per layer (stage)." Yet the definition of a block, "Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections."? Every stage represent a new block in which slides are cut.? These two statements are contradictory and need clarification. Now, my own opinion (again I have talked with my dermatopathologist and billing specialist and they are as lost as we) is that by definition, Mohs is a frozen section diagnosis that must be made by the surgeon (i.e., for a Mohs to be a mohs the surgeon removing the tissue must diagnose the tissue -- look it up).? Every section taken, at every stage is a separate block of the same case.? In the event you can charge immunos per case, only one charge can be made.? If it can be shown that immunos can be charged per block (per the definition below), every immuno on every block from every stage can be charged. Now for the practicality -- we always start questions like this because medicare sets standards for billing that other insurance companies then adopt.? We should NEVER ask, "what can we charge for," but should always ask, "what work did we do that it is fair for a patient to pay for."? Ignore what medicare and insurance companies say, bill clients for the work we perform and for the results they get.? How much more raw cost is there in staining two Mohs blocks with the same immuno?? Is it fair to charge a patient double the amount for MUCH less than twice the work? Will Chappell HTL(ASCP), QIHC On Jun 19, 2012, at 9:15 PM, Kim Donadio wrote: > Great team work! Job well done and a absolute answer is given. >? > Thank you > > > ________________________________ > From: Carol Torrence > To: 'Kim Donadio' > Cc: "'Weems, Joyce K.'" ; 'Ingles Claire' ; histonet@lists.utsouthwestern.edu > Sent: Tuesday, June 19, 2012 2:10 PM > Subject: RE: [Histonet] Billing IHC on MOHS > > > The following is the response I recived from a coding specialist at the American Academy of Dermatology.? I am trying not to be concerned that the reference is 6 years old but I think it clears up what we thought to be true.? > 88342 for IHC > 88314 other ?special stains? > Here is the description for 88314 according to November 2006 cpt Assistant article, the companion piece to the AMA CPT Code Book. > The work of processing and interpreting one routine stain is included in the procedure 17311- 17315. This stain is usually hematoxylin and eosin, or toluidine blue. If other special stains are necessary after one routine stain, then the code for special stains may be used (88314) as well as immunoperoxidase stains (88342) or decalcification procedures (88311). Special stains are not typically used and in most Mohs practices are of low frequency. Each stain is reported only once per block, not per slide or per layer (stage). > AMA CPT definition of a Block:Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From werley <@t> pitt.edu Wed Jun 20 07:25:48 2012 From: werley <@t> pitt.edu (Jonette Werley) Date: Wed Jun 20 07:25:54 2012 Subject: [Histonet] MGMT antibody Message-ID: I am using MGMT , clone MT23.2 from Invitrogen and having problems. Does anyone have a protocol for this antibody that is working and yielding consistent positive results? Thanks! From Carol.Freeman <@t> utoledo.edu Wed Jun 20 08:46:06 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Wed Jun 20 08:46:32 2012 Subject: [Histonet] Eosin staining for small biopsies In-Reply-To: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu> References: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu> Message-ID: Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. From lblazek <@t> digestivespecialists.com Wed Jun 20 09:01:03 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jun 20 09:01:45 2012 Subject: [Histonet] RE: Eosin staining for small biopsies In-Reply-To: References: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E3913E7C53872@IBMB7Exchange.digestivespecialists.com> We use eosin in our last 100% alcohol on the processor. It is a great help for our person embedding to be able to see and orient the small biopsies. We have been doing this for years and have never had any problem with the processor or any subsequent staining that has been performed. We also at times ink translucent esophageal biopsies at the grossing station to help in embedding. This also has never presented a problem. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol Sent: Wednesday, June 20, 2012 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin staining for small biopsies Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GaleL <@t> unionhospital.org Wed Jun 20 09:10:35 2012 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Wed Jun 20 09:10:47 2012 Subject: [Histonet] formalin substitute Message-ID: Good Morning, I would appreciate opinions and pricing on formalin substitute from anyone who is using it. We will be supplying our OB Dept. with this since they have trouble handling regular formalin....... Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From LPaveli1 <@t> hurleymc.com Wed Jun 20 09:11:41 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jun 20 09:11:54 2012 Subject: [Histonet] RE: Eosin staining for small biopsies In-Reply-To: References: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu>, Message-ID: <89F4666A496DC949A819ECC40E11C8670C33DB@EXCHANGEMB1.hmc.hurleymc.com> We also add liquid eosin (~75mL) to our "dirtiest" 100% to help aide the sight of the small biopsies for embedding. You are correct though, that eosin does fluoresce. I understand why you would be concerned about FISH, so wanted to share this. I have heard from fellow techs in the field that use a common bluing agent (used for laundry!) called Mrs. Stewart's Concentrated Liquid Bluing (found locally at a discount store called Meijers). It is applied at the grossing board. These techs actually use it for skin, but the bluing withstands the processor and helps them embed the skins much easier by showing the epidermis. I do believe that this harmless liquid does not fluoresce. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Freeman, Carol [Carol.Freeman@utoledo.edu] Sent: Wednesday, June 20, 2012 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin staining for small biopsies Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Wed Jun 20 09:19:09 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jun 20 09:19:19 2012 Subject: [Histonet] RE: formalin substitute In-Reply-To: References: Message-ID: <89F4666A496DC949A819ECC40E11C8670C33FC@EXCHANGEMB1.hmc.hurleymc.com> We have had great success using Prefer from Anatech. We have used it for outside locations with bone marrow aspirations. It is formaldelyde-free, and comes in either pre-filled containers or by larger containers. Our pathologists were happy with the nuclear detail.....and when your docs are happy, it's a happy day!!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gale Limron [GaleL@unionhospital.org] Sent: Wednesday, June 20, 2012 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin substitute Good Morning, I would appreciate opinions and pricing on formalin substitute from anyone who is using it. We will be supplying our OB Dept. with this since they have trouble handling regular formalin....... Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Wed Jun 20 09:31:40 2012 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Wed Jun 20 09:31:52 2012 Subject: [Histonet] Eosin staining for small biopsies In-Reply-To: References: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu> Message-ID: <4FE1987C020000AF0000951E@nodcdmg2.no.trinity-health.org> We use safranin (used in Microbiology as a counterstain) on our small biopsies. We apply a small drop during grossing. It does not affect staining of H&E, IHC or ISH. We like this because it is an intense red that doesn't leach out in the alcohols of processor. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Freeman, Carol" 6/20/2012 8:46 AM >>> Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Wed Jun 20 09:31:32 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Jun 20 09:31:55 2012 Subject: [Histonet] 3rd Shift HT/HTL Opening Near Fort Myers, FL Message-ID: Allied Search Partners is currently looking for a qualified applicant for a Histotechnologist or Histotechnician willing to work the third shift within a Fort Myers, FL laboratory. Position: Histotechnologist or Histotechnician Schedule: Monday-Friday, Overnight hours/Third Shift hours. Summary: Perform a variety of routine and specialized histology techniques and procedures. Embedding, Microtomy, Grossing, Processing, and H&E staining Special Staining Equipment maintenance Requirements: FL Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science preferred but not required Benefits: Competitive salaries, Health, Dental, Life & Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To Apply: Please send resume to brannon@alliedsearchpartners.com -- Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 20 10:29:28 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jun 20 10:29:14 2012 Subject: [Histonet] RE: Eosin staining for small biopsies In-Reply-To: References: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu> Message-ID: <8D7C2D242DBD45498006B21122072BF8B5776BA4@MCINFRWEM003.ucsfmedicalcenter.org> Here is a paper I found: Capillary Electrophoresis Artifact Due to Eosin Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005 Our molecular people are studying this issue to see how big of a problem it is. Tim Morken Supervisor, Electron Microscopy Department of Pathology UC San Francisco Medical Center tim.morken@ucsfmedctr.org Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol Sent: Wednesday, June 20, 2012 6:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin staining for small biopsies Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Wed Jun 20 11:07:12 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Wed Jun 20 11:07:35 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <1340190958.44522.YahooMailNeo@web112302.mail.gq1.yahoo.com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> <002601cd4e46$d232e340$7698a9c0$@com> <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> <1340190958.44522.YahooMailNeo@web112302.mail.gq1.yahoo.com> Message-ID: <002201cd4efe$c13024f0$43906ed0$@com> I have nothing more to add regarding this subject but would like to address concerns expressed here that touch on fairness, frequency, cost and the abilities of techs and surgeons. This was a very rare incident involving scar tissue and tumor. Our Mohs lab does not do immunos, our pathology lab does. My quest was to learn more about what constitutes a block. We stained 5 slides that were the same ?stage? (one block)?.?to be sure there would be no ?fall offs?. We do not want to put the patient through more waiting than necessary. I was not trying to charge for each slide I stained or gouge the patient. My quest was for ?correct coding? not what ?can? I charge. I certified as a CPC in 2005 but do not practice in that field. That said, I have a good grasp as to the seriousness of the subject of coding and documentation. I have 30 plus years of experience in histology including management. I have always had the pleasure of working with physicians of high integrity and continue to do so in the area of dermatology. The majority of my time has been spent in a large medical center where coding questions could be addressed ?in house? so to speak. When I was consulted by the surgeon regarding coding this case, our search for the correct coding stems from the level of integrity we practice on a daily basis. I consulted the American Society for Mohs Surgery (ASMS) as was suggested and they do not offer coding advice. I was told that they are an administrative office and suggested that I contact AAD. Thanks for sharing your thoughts and listening to mine. Have a good day! Onward and upward! Carol M. Torrence, HT(ASCP)CM ctorrence@kmcpa.com Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you From araniqkslvr <@t> yahoo.com Wed Jun 20 11:15:06 2012 From: araniqkslvr <@t> yahoo.com (Paula) Date: Wed Jun 20 11:15:10 2012 Subject: [Histonet] Looking for a recruiter to work with me! Message-ID: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> Hello, I am looking for a recruiter to work with me in getting back into the field of histology. I have been out a long time but I know I can re-learn. I have an A.A. in histotechnology and I am HT certified. I need a recruiter that won't laugh at me (it happened in the past) and that will answer my emails. I am in Raleigh, North Carolina. I had one person totally interested but when he realized I was not a new graduate he wanted to get off the phone as fast as possible. I am willing to work at this, take a class or whatever, buy new textbooks and refresh my knowledge. There is no school nearby for me to re-take a clinical, or I would do it. Thanks, Paula From chapcl <@t> yahoo.com Wed Jun 20 11:17:41 2012 From: chapcl <@t> yahoo.com (William) Date: Wed Jun 20 11:17:53 2012 Subject: [Histonet] Looking for a recruiter to work with me! In-Reply-To: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> References: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> Message-ID: <425A09E7-7B63-4675-839A-4EE2B024C60A@yahoo.com> Call James Elliott at Ampian staffing. One of the best in the business. Knows histology in and out. (877) 229-6996 Will Sent from my iPhone On Jun 20, 2012, at 12:15 PM, Paula wrote: > Hello, > > I am looking for a recruiter to work with me in getting back into the field of histology. I have been out a long time but I know I can re-learn. I have an A.A. in histotechnology and I am HT certified. > > I need a recruiter that won't laugh at me (it happened in the past) and that will answer my emails. I am in Raleigh, North Carolina. I had one person totally interested but when he realized I was not a new graduate he wanted to get off the phone as fast as possible. > > I am willing to work at this, take a class or whatever, buy new textbooks and refresh my knowledge. There is no school nearby for me to re-take a clinical, or I would do it. > > Thanks, > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Wed Jun 20 11:43:13 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Jun 20 11:43:22 2012 Subject: [Histonet] Looking for a recruiter to work with me! In-Reply-To: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> References: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> Message-ID: Let me get this straight. You are an HT (ASCP) living in the North Carolina Research Triangle and you can't find a job? Hmmm, UNC Health Care in Chapel Hill, Duke Medicine in Durham, and Rex Healthcare in Raleigh are all looking for histotechs RIGHT NOW. And this was from a 5 second Google search. This is why the recruiter laughed at you, not your being out of the field. Any anxiety you feel about getting back in the lab is something you are doing to yourself. If you can cut, and the lab is busy enough, they won't care. By the way, we old timers are "registered" histotechs, not certified. Some of us are certifiable. Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) From cpyse <@t> x-celllab.com Wed Jun 20 11:42:28 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Jun 20 11:44:56 2012 Subject: [Histonet] Eosin staining for small biopsies In-Reply-To: <4FE1987C020000AF0000951E@nodcdmg2.no.trinity-health.org> References: <418b3599-26e0-4dc6-b6f5-749a4fa9e9eb@MsgApp11.utad.utoledo.edu> <4FE1987C020000AF0000951E@nodcdmg2.no.trinity-health.org> Message-ID: <003501cd4f03$aee5bbc0$0cb13340$@com> We also use safranin on all our small biopsies and also our breast biopsies with no adverse effects to any of our IHC or ISH. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Wednesday, June 20, 2012 10:32 AM To: histonet@lists.utsouthwestern.edu; Carol Freeman Subject: Re: [Histonet] Eosin staining for small biopsies We use safranin (used in Microbiology as a counterstain) on our small biopsies. We apply a small drop during grossing. It does not affect staining of H&E, IHC or ISH. We like this because it is an intense red that doesn't leach out in the alcohols of processor. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Freeman, Carol" 6/20/2012 8:46 AM >>> Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jun 20 12:19:11 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jun 20 12:19:19 2012 Subject: [Histonet] Looking for a recruiter to work with me! In-Reply-To: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> References: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> Message-ID: Recruiters can be cruel. I have had them laugh at me as well, but not for the same reasons I guess. My opinion is that the markets I have worked in, they are glad to get someone with any background, and certainly you have shown your willingness to do what it takes to re-enter. I think that you should find some opportunities out there. I usually find that the recruiters who were histotechs for awhile and then went into recruiting are better. You have to keep after some of them, some of them are more focused on just getting names to their clients and don't concern themselves with making sure you it is a good fit for you or the organization. I learn about positions through others a lot too. Try posting your profile targeting your geographic area of interest. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 20 Jun 2012 09:15:06 -0700 > From: araniqkslvr@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Looking for a recruiter to work with me! > > Hello, > > I am looking for a recruiter to work with me in getting back into the field of histology. I have been out a long time but I know I can re-learn. I have an A.A. in histotechnology and I am HT certified. > > I need a recruiter that won't laugh at me (it happened in the past) and that will answer my emails. I am in Raleigh, North Carolina. I had one person totally interested but when he realized I was not a new graduate he wanted to get off the phone as fast as possible. > > I am willing to work at this, take a class or whatever, buy new textbooks and refresh my knowledge. There is no school nearby for me to re-take a clinical, or I would do it. > > Thanks, > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Jun 20 12:40:29 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jun 20 12:40:32 2012 Subject: [Histonet] Re: Eosin staining for small biopsies Message-ID: Carol Freeman asks about using eosin and other dyes to mark small specimens for better recovery during embedding. I've found safranin O to be the best of these. Use the Gram stain counterstain used in microbiology. You mark the specimens directly, on those blue biopsy pads. It doesn't dissolve out, and supposedly it isn't fluorescent and doesn't interfere with fluorescence procedures such as FISH. - The disadvantage is that marking the specimens with it is time-consuming. I'd want it used in conjunction with a grossing log sheet that the embedder checks while embedding (dream on). Eosin is easy to put in the processor, but its fluorescence supposedly precludes its use, a serious drawback. Hematoxylin - I have no experience with it. It isn't fluorescent, but in some situations can quench fluorescence. Mrs. Stewart's Bluing - No experience with it. It's made by the same company that makes Davidson marking inks. I don't know if it's particulate. Davidson's marking inks: They do the job, but as a pathologist I find particulate material distracting when it's on the slide with a small biopsy specimen, so I'd rather they not be used, though obviously they're necessary for evaluating surgical margins on breast and skin cancer resection specimens. Only a minority of pathology labs do small specimen marking of any kind, but I think it's a good idea. I don't know of any refereed literature on this subject - don't know if it's covered in any of the standard books - I'm away from my library right now. Bob Richmond Samurai Pathologist Knoxville TN From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 20 13:13:24 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 20 13:13:28 2012 Subject: [Histonet] elastic stain Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4301A344B27CED@HPEMX3.HealthPartners.int> Why would an elastic stain work on a positive control, but the patient did not stain?? The patient tissue is from a temporal artery which should show some staining due to internal control?? Thanks ahead for any input on this puzzle! Dorothy Webb, HT (ASCP) ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From histotalk <@t> yahoo.com Wed Jun 20 19:10:59 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Jun 20 19:11:03 2012 Subject: [Histonet] formalin substitute In-Reply-To: References: Message-ID: <1340237459.57397.YahooMailNeo@web121504.mail.ne1.yahoo.com> Hi Gale - For more than seventeen years we used a product called HistoChoice from Amresco http://www.amresco-inc.com/ . It's worth checking out. ? Dave Kemler Histology Consultant ________________________________ From: Gale Limron To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, June 20, 2012 10:10 AM Subject: [Histonet] formalin substitute Good Morning, I would appreciate opinions and pricing on formalin substitute from anyone who is using it. We will be supplying our OB Dept. with this since they have trouble handling regular formalin....... Thanks, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jun 20 20:06:34 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jun 20 20:06:38 2012 Subject: [Histonet] Billing IHC on MOHS In-Reply-To: <002201cd4efe$c13024f0$43906ed0$@com> References: <000301cd4a5c$b8a09230$29e1b690$@com> <064F1ACBAE8A78469AE2E41D533D87E505A85A@UWHC-MAIL2.uwhis.hosp.wisc.edu> <000301cd4b23$860d9cb0$9228d610$@com> <7A2BFC32-47C0-4017-ADFD-05F624CB4FD3@yahoo.com> <002201cd4d98$8adfc160$a09f4420$@com> <1340056950.83367.YahooMailNeo@web112305.mail.gq1.yahoo.com> <003301cd4da1$d5872650$809572f0$@com> <002601cd4e46$d232e340$7698a9c0$@com> <1340154949.25024.YahooMailNeo@web112305.mail.gq1.yahoo.com> <1340190958.44522.YahooMailNeo@web112302.mail.gq1.yahoo.com> <002201cd4efe$c13024f0$43906ed0$@com> Message-ID: Carol, Its nice to hear this isn't a regular thing. In reading your original question, it sounded like you were excited to be charging five times for those immunos and you were ready to argue for it. Apparently that was not the case, you wanted more information and thoughts on the subject. I once worked for a MOHS surgeon who had billing practices with which I did not agree. It was the reason I quit. I'm glad there are people of high integrity, such as your group, doing this work. Mark On Wednesday, June 20, 2012, Carol Torrence wrote: > I have nothing more to add regarding this subject but would like to > address concerns expressed here that touch on fairness, frequency, cost and > the abilities of techs and surgeons.**** > > ** ** > > This was a very rare incident involving scar tissue and tumor. Our Mohs > lab does not do immunos, our pathology lab does. My quest was to learn > more about what constitutes a block. We stained 5 slides that were the > same ?stage? (one block)?.?to be sure there would be no ?fall offs?. We do > not want to put the patient through more waiting than necessary. I was > not trying to charge for each slide I stained or gouge the patient. My > quest was for ?correct coding? not what ?can? I charge. I certified as a > CPC in 2005 but do not practice in that field. That said, I have a good > grasp as to the seriousness of the subject of coding and documentation.*** > * > > ** ** > > I have 30 plus years of experience in histology including management. I > have always had the pleasure of working with physicians of high integrity > and continue to do so in the area of dermatology. The majority of my time > has been spent in a large medical center where coding questions could be > addressed ?in house? so to speak. When I was consulted by the surgeon > regarding coding this case, our search for the correct coding stems from > the level of integrity we practice on a daily basis. I consulted the *American > Society for Mohs Surgery* (*ASMS*) as was suggested and they do not offer > coding advice. I was told that they are an administrative office and > suggested that I contact AAD.**** > > ** ** > > Thanks for sharing your thoughts and listening to mine.**** > > ** ** > > Have a good day! Onward and upward!**** > > Carol M. Torrence, HT(ASCP)CM **** > > ctorrence@kmcpa.com *** > * > > ** ** > > Confidentiality Note: This message is intended for use only by the > individual or entity to which it is addressed and may contain information > that is privileged, confidential, and exempt from disclosure under > applicable law. If the reader of this message is not the intended recipient > or the employee or agent responsible for delivering the message to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. If > you have received this communication in error, please contact the sender > immediately and destroy the material in its entirety, whether electronic or > hard copy. Thank you**** > > ** ** > > ** ** > > * ***** > From louise.renton <@t> gmail.com Thu Jun 21 04:20:34 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Thu Jun 21 04:52:53 2012 Subject: [Histonet] Klinipath coverslipper Message-ID: Hello Histoworld! Has anyone used the Klinipath coverslipper? I am thinking of buying one and was wondering how it compares to the Sakura tape coverslippper. Thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From relia1 <@t> earthlink.net Thu Jun 21 10:01:23 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jun 21 10:01:32 2012 Subject: [Histonet] Looking for a recruiter to work with me! In-Reply-To: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> References: <1340208906.53850.YahooMailClassic@web160203.mail.bf1.yahoo.com> Message-ID: <00ab01cd4fbe$b9cdb860$2d692920$@earthlink.net> Hi Paula and fellow histonetters! Please allow me to put my 2 cents in on this conversation. The most important thing I want to say to you is please don't shoot the messenger! Secondly, if a recruiter or anyone in a professional setting is asked for help and responds by laughing, me personally I would run screaming. As a matter of fact can you think of any situation where it would be an appropriate response to laugh when someone asks for help of any kind? That being said, This economy has changed the rules for EVERYBODY. If you had contacted me or any histology recruiter anytime prior to the fall of 2008 you probably would have gotten a job since you have kept up your ascp license, certification, registry, whichever you prefer. Now, a lot of employers have the policy that if you have not worked in the last 12 months they don't want to see your resume - from a recruiter. This directive is coming from lab managers and histology managers not HR. When an employer engages a recruiter today they expect that they are paying a fee to the recruiter for someone who can come in and hit the ground running. Usually they are short staffed and don't have the time to retrain or wait for someone to get up to speed. Especially if they are paying a fee for that person. Your best bet is to apply to jobs directly. Unfortunately that won't be easy either since their first priority regardless of whether there is a fee involved is to get someone who has recent experience and in this economy you are competing with experienced techs and new graduates of histology schools. My advice to you Paula is to look on your own and even apply for histology lab assistant positions just to get your foot in the door. Don't give up. Things are finally starting to pick up. Thanks-Pam Thank You! ? ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sent: Wednesday, June 20, 2012 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for a recruiter to work with me! Hello, I am looking for a recruiter to work with me in getting back into the field of histology. I have been out a long time but I know I can re-learn. I have an A.A. in histotechnology and I am HT certified. I need a recruiter that won't laugh at me (it happened in the past) and that will answer my emails. I am in Raleigh, North Carolina. I had one person totally interested but when he realized I was not a new graduate he wanted to get off the phone as fast as possible. I am willing to work at this, take a class or whatever, buy new textbooks and refresh my knowledge. There is no school nearby for me to re-take a clinical, or I would do it. Thanks, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Thu Jun 21 10:29:27 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jun 21 10:29:36 2012 Subject: [Histonet] turnaround time Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E1DC7C@PEITHA.wad.pa-ucl.com> Histonetters- As we discuss new instrumentation and different workflow scenarios I am curious to know what kind of turnaround time others have from time of accession to report sign out. 1. How long and what percentage? 2. How many have HT's grossing in smalls (skins, gi's)? Thank you much! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Thu Jun 21 11:06:48 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 21 11:06:52 2012 Subject: [Histonet] turnaround time In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E1DC7C@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E1DC7C@PEITHA.wad.pa-ucl.com> Message-ID: <1340294808.42913.YahooMailNeo@web121401.mail.ne1.yahoo.com> Your TAT will be determined by the way you organize your workflow. Under separate cover I am sending you data for several countries that I presented at a conference in Australia and another in Spain. Ren? J. ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 21, 2012 11:29 AM Subject: [Histonet] turnaround time Histonetters- As we discuss new instrumentation and different workflow scenarios I am curious to know what kind of turnaround time others have from time of accession to report sign out. 1.? How long and what percentage? 2.? How many have HT's grossing in smalls (skins, gi's)? Thank you much! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Jun 21 11:16:53 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jun 21 11:14:38 2012 Subject: [Histonet] turnaround time In-Reply-To: <1340294808.42913.YahooMailNeo@web121401.mail.ne1.yahoo.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36E1DC7C@PEITHA.wad.pa-ucl.com> <1340294808.42913.YahooMailNeo@web121401.mail.ne1.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570712989F47@smcmail02.somerset-healthcare.com> You also might do better calculating your end time not as signed out, but delivered to the pathologist. By doing this, you can get a better understanding of where the bottlenecks occur. Your techs may get the slides out at 9:00, but the case may not be signed out for another 8 hours. We have PAs for the grossing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 21, 2012 12:07 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] turnaround time Your TAT will be determined by the way you organize your workflow. Under separate cover I am sending you data for several countries that I presented at a conference in Australia and another in Spain. Ren? J. ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 21, 2012 11:29 AM Subject: [Histonet] turnaround time Histonetters- As we discuss new instrumentation and different workflow scenarios I am curious to know what kind of turnaround time others have from time of accession to report sign out. 1.? How long and what percentage? 2.? How many have HT's grossing in smalls (skins, gi's)? Thank you much! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. 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From TJohnson <@t> gnf.org Thu Jun 21 12:45:18 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jun 21 12:45:25 2012 Subject: [Histonet] Adopting a bar code system Message-ID: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From TJohnson <@t> gnf.org Thu Jun 21 12:50:40 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jun 21 12:50:49 2012 Subject: [Histonet] Happy Retirement to Jim Burchette In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDAFFFD@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDAFFFD@EX5.lj.gnf.org> Message-ID: <9F3CFEE76E51B64991C7485270890B400CDB0022@EX5.lj.gnf.org> I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. Best wishes JB! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From TJohnson <@t> gnf.org Thu Jun 21 13:26:16 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jun 21 13:26:19 2012 Subject: [Histonet] Re: Adopting a bar code system Message-ID: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org> Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turnelin <@t> HHSC.CA Thu Jun 21 13:32:31 2012 From: turnelin <@t> HHSC.CA (Turner-Smith Linda) Date: Thu Jun 21 13:32:35 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> Message-ID: Make sure that if the system produces slide labels that go on at the time of microtomy (before staining) that they are compatible with your automatic cover slipper. If the label is even the slightest bit too big the machine may not be able to pick up the slides properly. Big Trouble ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From arvidsonkristen <@t> yahoo.com Thu Jun 21 13:48:49 2012 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Thu Jun 21 13:48:54 2012 Subject: [Histonet] Need AFB controls Message-ID: <1340304529.91188.YahooMailClassic@web162101.mail.bf1.yahoo.com> Anyone out there have any spare AFB controls?? Thanks is advance :) From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jun 21 13:55:19 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jun 21 13:56:26 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org> Message-ID: Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Jun 21 13:57:31 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Jun 21 13:57:39 2012 Subject: [Histonet] Adopting a bar code system In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> Message-ID: <002201cd4fdf$b78e73c0$26ab5b40$@pathview.com> Teri, if you don't mind, I'd like to respond from the perspective of an LIS vendor. I would propose that you need to think about what your objectives are when implementing such a system, and then seek out those vendors who can best help you to reach those goals. Barcoding is a fairly straight forward and simple thing to do, but it can open so many doors that it's hard to choose what doors to actually go down. At its most simplistic representation, you're putting a barcode on the requisition, specimen, block, and slide. By doing so you give yourself the POTENTIAL to records dates/times/userid of who handled what material at any given time. Once you know this information, you can identify bottlenecks in your workflow and design more efficient workflows. Your objectives come into play when you try to choose vendors to implement the system. For instance, 1. What times/processes are you measuring? Is it just the steps from grossing to embedding to cutting to slide distribution? I believe this is what most people think of when they think of a 'bar code system', and you can find solutions from various LIS vendors and middleware products from Leica, Ventana, Lablion, and more. 2. Do you want to track other 'waypoints' in the process? For instance, into and out of strainers, processors, shipping, pathologist diagnosis, etc.? 3. Do you want to turn tracking on and off? 4. What management and statistical reports do you want to see? You need to keep in mind that it would be very nice to create your own reports in addition to having access to standard reports because management is always asking to look at the data from a different perspective. 5. A key tangent of barcode tracking is the ability to log alerts/commentary/quality issues. Does the proposed tracking system provide this capability? How easy is it to see these notations? Does the pathologist need to see them? 6. For laboratories who have to process cytology specimen, does the system help you track paps, nongyns, etc.? I could go on for quite a bit on this subject. There are of course, the real world issues of slide labels vs printing directly on slides, barcode misreads, compatibility with instrumentations and so on. If you'd like to talk more, please contact me offline. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Thu Jun 21 14:53:12 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jun 21 14:53:17 2012 Subject: [Histonet] Adopting a bar code system In-Reply-To: <002201cd4fdf$b78e73c0$26ab5b40$@pathview.com> References: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> <002201cd4fdf$b78e73c0$26ab5b40$@pathview.com> Message-ID: <9F3CFEE76E51B64991C7485270890B400CDB00E2@EX5.lj.gnf.org> Mike, This is a great overview of questions that need to be answered when considering such a system. Our current needs are fairly simple but I am also wanting to be proactive about potential future use. I appreciate your email to the list; it is useful for those of us as we negotiate this process. Teri -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Thursday, June 21, 2012 11:58 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Adopting a bar code system Teri, if you don't mind, I'd like to respond from the perspective of an LIS vendor. I would propose that you need to think about what your objectives are when implementing such a system, and then seek out those vendors who can best help you to reach those goals. Barcoding is a fairly straight forward and simple thing to do, but it can open so many doors that it's hard to choose what doors to actually go down. At its most simplistic representation, you're putting a barcode on the requisition, specimen, block, and slide. By doing so you give yourself the POTENTIAL to records dates/times/userid of who handled what material at any given time. Once you know this information, you can identify bottlenecks in your workflow and design more efficient workflows. Your objectives come into play when you try to choose vendors to implement the system. For instance, 1. What times/processes are you measuring? Is it just the steps from grossing to embedding to cutting to slide distribution? I believe this is what most people think of when they think of a 'bar code system', and you can find solutions from various LIS vendors and middleware products from Leica, Ventana, Lablion, and more. 2. Do you want to track other 'waypoints' in the process? For instance, into and out of strainers, processors, shipping, pathologist diagnosis, etc.? 3. Do you want to turn tracking on and off? 4. What management and statistical reports do you want to see? You need to keep in mind that it would be very nice to create your own reports in addition to having access to standard reports because management is always asking to look at the data from a different perspective. 5. A key tangent of barcode tracking is the ability to log alerts/commentary/quality issues. Does the proposed tracking system provide this capability? How easy is it to see these notations? Does the pathologist need to see them? 6. For laboratories who have to process cytology specimen, does the system help you track paps, nongyns, etc.? I could go on for quite a bit on this subject. There are of course, the real world issues of slide labels vs printing directly on slides, barcode misreads, compatibility with instrumentations and so on. If you'd like to talk more, please contact me offline. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Thu Jun 21 16:47:18 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Jun 21 16:47:21 2012 Subject: [Histonet] Elastic Stain Message-ID: Hi Dorothy, Controls for Verhoeff vanGieson elastic stain is very subjective due to the differentiation in ferric chloride. Large arteries are usually used as a control. If you differentiate all the slides based upon the appearance of a large artery the small vessels will be totally gone. If you use a small vessel as a control you will have underdifferentiated large artery. Every slide should be differentiated individually for optimal results. Yes, it is laborious, but the end result is well worth it. All the best, Amos From Timothy.Morken <@t> ucsfmedctr.org Thu Jun 21 16:54:40 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jun 21 16:54:23 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDAFFF0@EX5.lj.gnf.org> Message-ID: <8D7C2D242DBD45498006B21122072BF8B57FE3DF@MCINFRWEM003.ucsfmedicalcenter.org> Hi Teri, First, is this for research or clinical? The two workflows and needs may be entirely different. The major systems on the market for clinical histology may not work very well in a research setting as they are aimed at streamlining the normal histology workflow and are fairly rigid in their structure. Research usually has much more going on and many more branches to consider and you will want a system that is flexible and customizable. Other systems that are designed for research warrant investigation. Second, if you are starting from coding the cassettes, then the cassette is going to be the critical link to the histology lab and you must do everything possible to get the very best result on cassette printing. Otherwise you will build in failure downstream. There are pros and cons to each cassette printer - some have extra bulky components that may not fit your lab. Some are big, but fast, others small but slow. You have to fit them to your lab. Downstream you have to think about how you are going to use coding in each section, what room you have for computers, monitors, scanners etc. It adds up. Also look into the infrastructure for data wiring. That is often a limitation that has to be addressed. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 10:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Fri Jun 22 05:40:35 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Jun 22 05:41:59 2012 Subject: [Histonet] RE: Happy Retirement to Jim Burchette In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDB0022@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDAFFFD@EX5.lj.gnf.org> <9F3CFEE76E51B64991C7485270890B400CDB0022@EX5.lj.gnf.org> Message-ID: Best of Luck to Mr. Burchette. Who, I am sure, doesn't know me from Eve, but once sent me a positive control block. I didn't ask for anything in return. I was happy & astonished. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Happy Retirement to Jim Burchette I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. Best wishes JB! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcampbell <@t> trianglebiomedical.com Fri Jun 22 06:26:39 2012 From: dcampbell <@t> trianglebiomedical.com (Dustin Paul Campbell) Date: Fri Jun 22 06:26:47 2012 Subject: [Histonet] RE: Happy Retirement to Jim Burchette Message-ID: <4c429724b868a055f4344d97058eac3c19970454@localhost> Jim, On behalf of everyone at TBS, happy retirement and thank you for all your insight. Your assistance in product development has helped us tremendously. Dustin Campbell?? 3014 Croasdaile Drive, Durham?? NC? 27705? ?p 919.384.9393 ? f 919.384.9595 dcampbell@trianglebiomedical.com? Visit us online at www.trianglebiomedical.com and follow us on -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Friday, June 22, 2012 6:41 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Happy Retirement to Jim Burchette Importance: High Best of Luck to Mr. Burchette. Who, I am sure, doesn't know me from Eve, but once sent me a positive control block. I didn't ask for anything in return. I was happy & astonished. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Happy Retirement to Jim Burchette I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. Best wishes JB! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> CSILaboratories.com Fri Jun 22 07:10:44 2012 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Jun 22 07:10:51 2012 Subject: [Histonet] RE: Happy Retirement to Jim Burchette In-Reply-To: <4c429724b868a055f4344d97058eac3c19970454@localhost> References: <4c429724b868a055f4344d97058eac3c19970454@localhost> Message-ID: <467983BDAC5880438E2202F6B9BF79A88C69C8@exchange-be-01.CSI-LABS.local> Happy retirement, Jim, and thanks for all you did for our field, but especially for the patients whose lives you enriched! Enjoy a well-deserved retirement! Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: Dustin Paul Campbell [mailto:dcampbell@trianglebiomedical.com] Sent: Friday, June 22, 2012 7:27 AM To: Settembre, Dana; Teri Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Happy Retirement to Jim Burchette Jim, On behalf of everyone at TBS, happy retirement and thank you for all your insight. Your assistance in product development has helped us tremendously. Dustin Campbell?? 3014 Croasdaile Drive, Durham?? NC? 27705? ?p 919.384.9393 ? f 919.384.9595 dcampbell@trianglebiomedical.com? Visit us online at www.trianglebiomedical.com and follow us on -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Friday, June 22, 2012 6:41 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Happy Retirement to Jim Burchette Importance: High Best of Luck to Mr. Burchette. Who, I am sure, doesn't know me from Eve, but once sent me a positive control block. I didn't ask for anything in return. I was happy & astonished. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Thursday, June 21, 2012 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Happy Retirement to Jim Burchette I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. Best wishes JB! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkrichar <@t> gundluth.org Fri Jun 22 08:46:18 2012 From: pkrichar <@t> gundluth.org (Richardson, Pam K) Date: Fri Jun 22 08:51:54 2012 Subject: [Histonet] Automated special Stains Message-ID: <998284C32F61104CA0BEFFFFCF6F90FDE86066@LXEXMB03.gundluth.org> We are interested in an automated special stainer. What are you using? Pros? Cons? Thanks Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org From rennie1108 <@t> yahoo.com Fri Jun 22 08:09:00 2012 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Fri Jun 22 08:53:58 2012 Subject: [Histonet] Happy Retirement to Jim Burchette In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDB0022@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDAFFFD@EX5.lj.gnf.org> <9F3CFEE76E51B64991C7485270890B400CDB0022@EX5.lj.gnf.org> Message-ID: Jim is such an amazing person and an expert in his field. He will be missed in the Immuno lab, I'm sure! Congrats, Jim! Sounds like you'll be able to enjoy some more fly fishing time!! On Jun 21, 2012, at 1:50 PM, Teri Johnson wrote: > I am making this official and inviting all of you who know Jim to join me in wishing him a very happy retirement. His impact to our field and to the patient care at Duke University has been stellar. I had no idea his feet were so big gauging by the size of shoe left behind to fill. > > Best wishes JB! > > Teri Johnson, HT(ASCP)QIHC > GNF Histology Lab Manager > Genomics Institute of the Novartis Research Foundation > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 22 09:08:34 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 22 09:08:41 2012 Subject: [Histonet] Automated special Stains In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FDE86066@LXEXMB03.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FDE86066@LXEXMB03.gundluth.org> Message-ID: <1340374114.94355.YahooMailNeo@web121405.mail.ne1.yahoo.com> At this moment most histology equipment manufacturers offer special stainers but not all perform the same or are equally reliable. The one manufactured by DAKO is very good and you will receive many comments about many others. The automated stainer by Sakura is very good and reliable when you have numerous sections to be stained with some special procedure. Try to make your decision mostly on the quality of?service when "something goes wrong" and the price. Ren? J. ________________________________ From: "Richardson, Pam K" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, June 22, 2012 9:46 AM Subject: [Histonet] Automated special Stains We are interested in an automated special stainer. What are you using? Pros? Cons? Thanks Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 22 09:17:28 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 22 09:17:39 2012 Subject: [Histonet] RE: Automated special Stains In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FDE86066@LXEXMB03.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FDE86066@LXEXMB03.gundluth.org> Message-ID: We use the DAKO Artisan and highly recommend it! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richardson, Pam K Sent: Friday, June 22, 2012 9:46 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Automated special Stains We are interested in an automated special stainer. What are you using? Pros? Cons? Thanks Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From shanonpink <@t> gmail.com Fri Jun 22 09:57:07 2012 From: shanonpink <@t> gmail.com (Shanon Pink) Date: Fri Jun 22 09:57:14 2012 Subject: [Histonet] Tolivia and Navarro Epoxy Staining Procedure Message-ID: Hello All, Has anyone out there tried the staining procedure described in the paper "Polychromatic staining of epoxy semithin sections: a new and simple method," from 1994, by Tolivia, Navarro, and Tolivia? I would like to try it out on some thick sections embedded in Epon, but I can't find any carbol methylene blue and wondered about possible substitutions. Thanks in advance for any help anyone can offer! Shanon Pink University of Tennessee Health Science Center Memphis, TN From Donna.Willis <@t> baylorhealth.edu Fri Jun 22 09:58:02 2012 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Jun 22 09:58:11 2012 Subject: [Histonet] Embedding beads Message-ID: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 22 10:03:22 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 22 10:03:35 2012 Subject: [Histonet] RE: Embedding beads In-Reply-To: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> Message-ID: I would get them from a craft shop or a bead shop - much cheaper! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, June 22, 2012 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsfmedctr.org Fri Jun 22 10:15:22 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jun 22 10:15:06 2012 Subject: [Histonet] RE: Embedding beads In-Reply-To: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> Message-ID: <8D7C2D242DBD45498006B21122072BF8B57FE686@MCINFRWEM003.ucsfmedicalcenter.org> Donna, Cancer Diagnostics has them, numbers 0 thru 9 and letters A thru E. Note, however, that the "6" and "9" are identical! They run about 2 cents per bead and come in bottles of 500. They are flat on two sides so don't roll around. We've been using them for about 6 months and everyone is happy with them. We tried confetti but everyone complained about how hard it was to pick up the pieces. Plus shapes and colors are not as easy to decode as numbers. We have a chart posted in the lab with each persons number or letter so it is really easy to determine who embedded a particular block. http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=109 Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, June 22, 2012 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Fri Jun 22 10:44:16 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Fri Jun 22 10:44:23 2012 Subject: [Histonet] Microm HM 500 OM Crytostat Message-ID: <3E6798F00C9F494399E96B720ECD1429083257@MSGWCDCPMB22.nyumc.org> Hi everyone Have been tasked with brining stored (?) Microm HM 500 OM Crytostat back into service. Does anyone happen to have the manual that they can either copy and or scan and send? Have searched online but have not come across the exact 500 OM manual (have seen 525, 550 500M + host of others) anyone have any comments or critiques about the platform please feel free to share... Thanks Happy weekend to all Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYUSOM Medical Science Building Room 124 646-501-6934 Luis.Chiriboga@nyumc.org From pkarlisch <@t> hmc.psu.edu Fri Jun 22 11:04:52 2012 From: pkarlisch <@t> hmc.psu.edu (Karlisch, Patricia) Date: Fri Jun 22 11:04:56 2012 Subject: [Histonet] James Burchette Message-ID: James, Thank you for your help in getting controls for us too! You are awesome and I wish you the best relaxing and wonderful retirement! Pat Karlisch *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From PAMarcum <@t> uams.edu Fri Jun 22 11:20:19 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Jun 22 11:20:29 2012 Subject: [Histonet] RE: James Burchette In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA324E386C4B@Mail2Node1.ad.uams.edu> Great fishing Jim!!! Pam ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Karlisch, Patricia [pkarlisch@hmc.psu.edu] Sent: Friday, June 22, 2012 11:04 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] James Burchette James, Thank you for your help in getting controls for us too! You are awesome and I wish you the best relaxing and wonderful retirement! Pat Karlisch *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From eca9 <@t> georgetown.edu Fri Jun 22 12:08:04 2012 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Fri Jun 22 12:08:17 2012 Subject: [Histonet] Looking for a published PLAP antibody that is not made in mouse Message-ID: <4FE4A674.7090501@georgetown.edu> Good afternoon, I have now spent way too much time trying to find a placental-like alkaline phosphatase antibody that is not made in mouse and has been published with pretty IHC-P pictures. All I keep finding are made in mouse. Could someone please direct me to a good one? I have found lots of rabbit made PLAP antibodies from companies but the investigator wants me to find one that has also been published. Thank you all for your help, Eva Permaul Georgetown University From Ronald.Houston <@t> nationwidechildrens.org Fri Jun 22 12:38:49 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jun 22 12:39:17 2012 Subject: [Histonet] Controls for IF Message-ID: What are people using to control direct IF? We use frozen tonsil for immunoglobulins and light chains, but what about fibrinogen, albumin, C1q, C3c and C4c? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From renafail2 <@t> gmail.com Fri Jun 22 12:54:39 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Fri Jun 22 12:54:43 2012 Subject: [Histonet] Jim Burchette Message-ID: No muss, no fuss just a generous heart always ready and willing to share. You made life easier in the lab because of your generosity. Thank you. Congratulations on your retirement! Rena Fail HRT (Happily retired Histotech) From kdboydhisto <@t> yahoo.com Fri Jun 22 14:20:46 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Fri Jun 22 14:20:49 2012 Subject: [Histonet] LIS vendors Message-ID: <1340392846.77211.YahooMailNeo@web125806.mail.ne1.yahoo.com> ?I have never?heard of?LABLION LIS systems. Anyone out there use it or might be familiar with it?? Kelly? From aj.taylor <@t> blueyonder.co.uk Fri Jun 22 17:14:31 2012 From: aj.taylor <@t> blueyonder.co.uk (Alan Taylor) Date: Fri Jun 22 17:16:41 2012 Subject: [Histonet] Embedding beads References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> Message-ID: <16ACFF4B2189457F8B050E8F35E777DC@merlin> Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make colourful place mats etc for children. Hamma beads come in a very wide range of colours, in bulk bags from any good toy or handicraft store. They are very cheap and they are clearly visible when placed in the corner of an embedding cassette when filling with wax. We have a small staff in our lab so the colours we have chosen are readily identifiable to an individual who has embedded a particular block. I can strongly recommend them, we have used them for a number of years as a traceable guide to a particular block. Hope this is helpful to all of you who are embedding lots of blocks each day. Best wishes to you all Alan Taylor BSc(Hons), FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ.UK ----- Original Message ----- From: "Willis, Donna G." To: Sent: Friday, June 22, 2012 3:58 PM Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DJenkins <@t> bwmc.umms.org Fri Jun 22 17:39:01 2012 From: DJenkins <@t> bwmc.umms.org (Jenkins, Dalena) Date: Fri Jun 22 17:38:30 2012 Subject: [Histonet] Job opening in Maryland Message-ID: <211C57CA9976E64AB2EFB3E7841DEE3003391162@mail2.nah.org> Baltimore Washington Medical Center has a full time Histology position opening. Hours are 8:30am to 5:00pm M-F with weekend rotation of on call for frozen sections. (about every 6th weekend) Interested applicants must have: Successful completion of CAHEA accredited Histotechnology program, OR Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university in biology, AND one year full time acceptable experience in Histopathology, OR HT (ASCP) registered or eligible. Prior laboratory experience is preferred. Knowledge of Immunohistochemistry is a plus. Applications must be made on line at mybwmc.org For more information you can email me at: djenkins@bwmc.umms.org From ibernard <@t> uab.edu Fri Jun 22 21:57:04 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Fri Jun 22 21:57:22 2012 Subject: [Histonet] Muscle Biopisy Procedure Message-ID: Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one. Since we never did one, we don't have a written procedure. We are looking for a benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week. Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From mbmphoto <@t> gmail.com Fri Jun 22 23:56:29 2012 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Fri Jun 22 23:56:45 2012 Subject: [Histonet] Jim Burchette - you are the very best! Message-ID: <39120543-3B36-450A-84D5-642A5C996944@gmail.com> Jim, I want to express my deepest appreciation for all your contribution at the annual NSH workshops and for your contribution on the Histonet. I feel very grateful and consider myself very fortunate for have taken some of your workshops. I also value your knowledge and insight of extensive histology experience and especially for your willingness to share it all with us in histology. All the best in your new life adventures. Maria Mejia San Francisco, California From max_histo_00 <@t> yahoo.it Sat Jun 23 01:33:19 2012 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sat Jun 23 01:33:23 2012 Subject: [Histonet] To Jim Burchette Message-ID: <1340433199.71259.YahooMailNeo@web29606.mail.ird.yahoo.com> Dear Jim, ? Thank you for your help. I wish you the best? relaxing and wonderful retirement! I do hope now you'll find, in the future, the time to come in Italy and we'll have the opportunity to meet? you and your family personally. My wife and I are waiting for you. My best wishes dear friend! Massimo Tosi From LPaveli1 <@t> hurleymc.com Sat Jun 23 09:23:05 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Sat Jun 23 09:23:12 2012 Subject: [Histonet] RE: Muscle Biopisy Procedure In-Reply-To: References: Message-ID: <89F4666A496DC949A819ECC40E11C8670C3673@EXCHANGEMB1.hmc.hurleymc.com> We have used M-Labs (University of Michigan, Ann Arbor) labs for over 20 years. Their protocol, complete with pictures is found online at: www.mlabs.umich.edu go to: Anatomic Pathology Nerve/muscle biopsy Muscle biopsy tissue submission protocol This protocol has never failed us!! hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Ian R Bernard [ibernard@uab.edu] Sent: Friday, June 22, 2012 10:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle Biopisy Procedure Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one. Since we never did one, we don't have a written procedure. We are looking for a benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week. Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.prior <@t> tissueregenix.com Mon Jun 25 04:49:57 2012 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Mon Jun 25 04:50:11 2012 Subject: [Histonet] Tendon Processing Message-ID: <833391A3F6889845BEDC18CEA1019F906683DC@tr-dc1.tissueregenix.local> Morning all, I am taking over some work on tendon tissue and would like some advice regarding processing schedules. The tissue is 3-5mm thick cross sections from large tendons and is formalin fixed, paraffin wax embedded. At the moment the sections (although flat and wrinkle free) appear cracked, which I think is due to excess dehydration. I would be very grateful if people could share their processing schedules so that I have an idea of where to adjust mine to improve tissue quality. Many Thanks. Andrew Prior Histologist Tissue Regenix Group The Biocentre York Science Park Innovation Way Heslington, York YO10 5NY E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com From shanonpink <@t> gmail.com Mon Jun 25 07:39:59 2012 From: shanonpink <@t> gmail.com (Shanon Pink) Date: Mon Jun 25 07:40:09 2012 Subject: [Histonet] Re: Tolivia, Navarro and Tolivia In-Reply-To: <000001cd50b8$366fbef0$a34f3cd0$@bresnan.net> References: <000001cd50b8$366fbef0$a34f3cd0$@bresnan.net> Message-ID: My apologies Gayle, I am new at this... >From Histochemistry (1994) 101:51-55, "Polychromatic staining of epoxy semithin sections: a new and simple method," by Tolivia, Navarro and Tolivia. I am wondering if anyone out there has tried this technique. Thank you for your time in responding! --Shanon On Fri, Jun 22, 2012 at 3:47 PM, gayle callis wrote: > What ?journal, year and issue for this publication? > > > > ******************** > > > > You wrote: > > > > Has anyone out there tried the staining procedure described in the paper > "Polychromatic staining of epoxy semithin sections: a new and simple > method," from 1994, by Tolivia, Navarro, and Tolivia? I would like to try it > out on some thick sections embedded in Epon, but I can't find any carbol > methylene blue and wondered about possible substitutions. > > > > Gayle Callis > > HTL/HT/MT(ASCP) From amber.mckenzie <@t> gastrodocs.net Mon Jun 25 08:43:05 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Jun 25 08:40:57 2012 Subject: [Histonet] Embedding beads In-Reply-To: <16ACFF4B2189457F8B050E8F35E777DC@merlin> References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> <16ACFF4B2189457F8B050E8F35E777DC@merlin> Message-ID: <5A33C952BB67F4468AF1F36D739212BC3082878B@JERRY.Gia.com> Does this slow the embedder down any having to pick a bead up each time to embed along with the tissue? I've never heard of this procedure before. The labs I've worked in have an embedding log and we initial each case we embed. What do you keep the beads in and are they set in a particular spot on the embedding station so they're easily assessable? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Taylor Sent: Friday, June 22, 2012 5:15 PM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Embedding beads Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make colourful place mats etc for children. Hamma beads come in a very wide range of colours, in bulk bags from any good toy or handicraft store. They are very cheap and they are clearly visible when placed in the corner of an embedding cassette when filling with wax. We have a small staff in our lab so the colours we have chosen are readily identifiable to an individual who has embedded a particular block. I can strongly recommend them, we have used them for a number of years as a traceable guide to a particular block. Hope this is helpful to all of you who are embedding lots of blocks each day. Best wishes to you all Alan Taylor BSc(Hons), FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ.UK ----- Original Message ----- From: "Willis, Donna G." To: Sent: Friday, June 22, 2012 3:58 PM Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Mon Jun 25 08:51:43 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Mon Jun 25 08:51:54 2012 Subject: [Histonet] Embedding beads In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC3082878B@JERRY.Gia.com> References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> <16ACFF4B2189457F8B050E8F35E777DC@merlin> <5A33C952BB67F4468AF1F36D739212BC3082878B@JERRY.Gia.com> Message-ID: We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab. There has to be accountability for every job. Jen Campbell On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > Does this slow the embedder down any having to pick a bead up each time to > embed along with the tissue? I've never heard of this procedure before. > The labs I've worked in have an embedding log and we initial each case we > embed. What do you keep the beads in and are they set in a particular spot > on the embedding station so they're easily assessable? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Taylor > Sent: Friday, June 22, 2012 5:15 PM > To: Willis, Donna G.; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Embedding beads > > Hi All > > We use Hamma beads to identify individual embedders on a particular day. > Hamma beads will be well known to parents and child carers as tiny tube > like > beads that can be made into many patterns and then ironed (by adults) to > make colourful place mats etc for children. > > Hamma beads come in a very wide range of colours, in bulk bags from any > good > toy or handicraft store. They are very cheap and they are clearly visible > when placed in the corner of an embedding cassette when filling with wax. > > We have a small staff in our lab so the colours we have chosen are readily > identifiable to an individual who has embedded a particular block. I can > strongly recommend them, we have used them for a number of years as a > traceable guide to a particular block. Hope this is helpful to all of you > who are embedding lots of blocks each day. > > Best wishes to you all > > Alan Taylor BSc(Hons), FRMS > Microtechnical Services > 71 Sweetbrier Lane > Heavitree > Exeter. Devon. EX1 3AJ.UK > > ----- Original Message ----- > From: "Willis, Donna G." > To: > Sent: Friday, June 22, 2012 3:58 PM > Subject: [Histonet] Embedding beads > > > Does anyone out in Histoland know of a vendor other that Mar Med to get > small beads to put in cassettes to designate the person that embeds. > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Histology Lab Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly forbidden and possibly a violation of federal or state law and > regulations. Baylor Health Care System, its subsidiaries, and affiliates > hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From shanonpink <@t> gmail.com Mon Jun 25 08:55:13 2012 From: shanonpink <@t> gmail.com (Shanon Pink) Date: Mon Jun 25 08:55:24 2012 Subject: [Histonet] Re: Tolivia, Navarro and Tolivia In-Reply-To: <000001cd52d8$60cf8a00$226e9e00$@bresnan.net> References: <000001cd50b8$366fbef0$a34f3cd0$@bresnan.net> <000001cd52d8$60cf8a00$226e9e00$@bresnan.net> Message-ID: Hi Again Gayle, I'm sorry I was not very clear; I have already downloaded and read the publication, and I am trying the procedure out today. I was wondering about using a couple of substitutions and thought I might ask if anyone has tried this procedure before. I am on a four-week research project trying to find a nice polychromatic stain to use for epon-embedded thick sections for researchers here at the EM lab at St. Jude Children's Research Hospital in Memphis. Normally they just use Toluidine Blue, but in some cases researchers would like to have better differentiation on their thick sections. Initially we were trying to find a stain that approximates H&E since that's a familiar stain, but I've only had mediocre results with the following stains: (1) Toluidine Blue/Sodium Borate, followed with Basic Fuchsin, and (2) a homemade Paragon stain. The staining procedure described in the aforementioned publication is performed at room temperature and is relatively fast. But as you point out, it involves phenol which is not fun to work with. Thanks to you and anyone else who has any thoughts! --Shanon Pink On Mon, Jun 25, 2012 at 8:42 AM, gayle callis wrote: > Shanon, > > Thank you. ? ?If you are at a university, you should be able to get this > publication on line if their library can do this for you. ? I am not sure I > can access it via my connections but will try later. ? There are other > methylene blue/basic fuchsin protocols that work very well on epoxy > sections. ?Keep in mind that the carbol component of this stain is just > another name for phenol, very toxic and often a controlled substance in > laboratories (can't be passed around!). > > If you want I will go into my EM file for the other MB/BF methods, they are > simple to use and make up. ? Let me know if you would like the publication. > > > Gayle Callis > > -----Original Message----- > From: Shanon Pink [mailto:shanonpink@gmail.com] > Sent: Monday, June 25, 2012 6:40 AM > To: gayle callis; histonet@lists.utsouthwestern.edu > Subject: Re: Tolivia, Navarro and Tolivia > > My apologies Gayle, I am new at this... > > >From Histochemistry (1994) 101:51-55, "Polychromatic staining of epoxy > semithin sections: a new and simple method," by Tolivia, Navarro and > Tolivia. I am wondering if anyone out there has tried this technique. > > Thank you for your time in responding! > > --Shanon > > On Fri, Jun 22, 2012 at 3:47 PM, gayle callis > wrote: >> What ?journal, year and issue for this publication? >> >> >> >> ******************** >> >> >> >> You wrote: >> >> >> >> Has anyone out there tried the staining procedure described in the >> paper "Polychromatic staining of epoxy semithin sections: a new and >> simple method," from 1994, by Tolivia, Navarro, and Tolivia? I would >> like to try it out on some thick sections embedded in Epon, but I >> can't find any carbol methylene blue and wondered about possible > substitutions. >> >> >> >> Gayle Callis >> >> HTL/HT/MT(ASCP) > From BDeBrosse-Serra <@t> isisph.com Mon Jun 25 09:16:50 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Jun 25 09:17:00 2012 Subject: [Histonet] RE: Muscle Biopsy Procedure In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F092A969BCB@EXCHMB01.isis.local> Ian, Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Friday, June 22, 2012 7:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle Biopisy Procedure Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one. Since we never did one, we don't have a written procedure. We are looking for a benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week. Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Jun 25 09:21:05 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jun 25 09:19:38 2012 Subject: [Histonet] Re: Tolivia, Navarro and Tolivia In-Reply-To: References: <000001cd50b8$366fbef0$a34f3cd0$@bresnan.net> <000001cd52d8$60cf8a00$226e9e00$@bresnan.net> Message-ID: <4FE873D1.6080209@umdnj.edu> I have sympathy for your dilemma! While I have no experience with the procedure in question there have been many, many publications since the 1960's trying to address this issue. Personally, I have never seen one I though was much good/worth the effort so I have just stayed with toluidine blue in borax. You might look at this paper by Charlotte Pool: /Pool, C.R. Hematoxylin-eosin staining of OsO4-fixed epon embedded tissue; prestaining oxidation by acidified H2O2. Stain Technol. 44:75-79, 1969./ Geoff On 6/25/2012 9:55 AM, Shanon Pink wrote: > Hi Again Gayle, > > I'm sorry I was not very clear; I have already downloaded and read the > publication, and I am trying the procedure out today. I was wondering > about using a couple of substitutions and thought I might ask if > anyone has tried this procedure before. > > I am on a four-week research project trying to find a nice > polychromatic stain to use for epon-embedded thick sections for > researchers here at the EM lab at St. Jude Children's Research > Hospital in Memphis. Normally they just use Toluidine Blue, but in > some cases researchers would like to have better differentiation on > their thick sections. Initially we were trying to find a stain that > approximates H&E since that's a familiar stain, but I've only had > mediocre results with the following stains: (1) Toluidine Blue/Sodium > Borate, followed with Basic Fuchsin, and (2) a homemade Paragon stain. > The staining procedure described in the aforementioned publication is > performed at room temperature and is relatively fast. But as you > point out, it involves phenol which is not fun to work with. > > Thanks to you and anyone else who has any thoughts! > > --Shanon Pink > > On Mon, Jun 25, 2012 at 8:42 AM, gayle callis wrote: >> Shanon, >> >> Thank you. If you are at a university, you should be able to get this >> publication on line if their library can do this for you. I am not sure I >> can access it via my connections but will try later. There are other >> methylene blue/basic fuchsin protocols that work very well on epoxy >> sections. Keep in mind that the carbol component of this stain is just >> another name for phenol, very toxic and often a controlled substance in >> laboratories (can't be passed around!). >> >> If you want I will go into my EM file for the other MB/BF methods, they are >> simple to use and make up. Let me know if you would like the publication. >> >> >> Gayle Callis >> >> -----Original Message----- >> From: Shanon Pink [mailto:shanonpink@gmail.com] >> Sent: Monday, June 25, 2012 6:40 AM >> To: gayle callis; histonet@lists.utsouthwestern.edu >> Subject: Re: Tolivia, Navarro and Tolivia >> >> My apologies Gayle, I am new at this... >> >> > From Histochemistry (1994) 101:51-55, "Polychromatic staining of epoxy >> semithin sections: a new and simple method," by Tolivia, Navarro and >> Tolivia. I am wondering if anyone out there has tried this technique. >> >> Thank you for your time in responding! >> >> --Shanon >> >> On Fri, Jun 22, 2012 at 3:47 PM, gayle callis >> wrote: >>> What journal, year and issue for this publication? >>> >>> >>> >>> ******************** >>> >>> >>> >>> You wrote: >>> >>> >>> >>> Has anyone out there tried the staining procedure described in the >>> paper "Polychromatic staining of epoxy semithin sections: a new and >>> simple method," from 1994, by Tolivia, Navarro, and Tolivia? I would >>> like to try it out on some thick sections embedded in Epon, but I >>> can't find any carbol methylene blue and wondered about possible >> substitutions. >>> >>> >>> Gayle Callis >>> >>> HTL/HT/MT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From algranth <@t> email.arizona.edu Mon Jun 25 10:13:41 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jun 25 10:13:49 2012 Subject: [Histonet] Embedding beads In-Reply-To: References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> <16ACFF4B2189457F8B050E8F35E777DC@merlin> <5A33C952BB67F4468AF1F36D739212BC3082878B@JERRY.Gia.com> Message-ID: I called on a lab in Las Vegas when I had my sales job that used sequins. Well, it was Vegas! Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From vtobias <@t> uw.edu Mon Jun 25 10:30:40 2012 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Mon Jun 25 10:31:25 2012 Subject: [Histonet] Embedding beads In-Reply-To: References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt> <16ACFF4B2189457F8B050E8F35E777DC@merlin> <5A33C952BB67F4468AF1F36D739212BC3082878B@JERRY.Gia.com> Message-ID: Another plus for barcoding and going paperless. Scanning the cassette logs who embedded the tissue and you can see what type of tissue it was, how many pieces should have been there and if there were any instructions such as on end or on edge. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, June 25, 2012 8:14 AM Cc: HISTONET Subject: Re: [Histonet] Embedding beads I called on a lab in Las Vegas when I had my sales job that used sequins. Well, it was Vegas! Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Jun 25 10:32:32 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Jun 25 10:32:40 2012 Subject: [Histonet] RE: Muscle Biopsy Procedure In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F092A969BCB@EXCHMB01.isis.local> References: <493CAA64F203E14E8823737B9EE0E25F092A969BCB@EXCHMB01.isis.local> Message-ID: <1340638352.57185.YahooMailNeo@web112314.mail.gq1.yahoo.com> One place I was at didnt get many muscle biopsies so we didnt keep liquid nitrogen and isopentane, which is what I was trained to use initially. Here's what we did when we got a muscle biopsy and it worked fine. ? After cutting the biopsy into 3-4 mm portions, we wrapped it in aluminum foil and snap froze it in a slush made from 100% alcohol and dry ice. We then shipped it in a styrofoam lined box full of peices of dry ice. The dry ice was made into small peices so we could place the snap frozen biopsy in the middle. ? Good luck! ________________________________ From: Bea DeBrosse-Serra To: 'Ian R Bernard' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, June 25, 2012 10:16 AM Subject: [Histonet] RE: Muscle Biopsy Procedure Ian, Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Friday, June 22, 2012 7:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle Biopisy Procedure Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one.? Since we never did one, we don't have a written procedure. We are looking for a? benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week.? Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Jun 25 10:33:51 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Jun 25 10:34:06 2012 Subject: [Histonet] RE: Muscle Biopsy Procedure In-Reply-To: <1340638352.57185.YahooMailNeo@web112314.mail.gq1.yahoo.com> References: <493CAA64F203E14E8823737B9EE0E25F092A969BCB@EXCHMB01.isis.local> <1340638352.57185.YahooMailNeo@web112314.mail.gq1.yahoo.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D500BF30E01@BL2PRD0710MB363.namprd07.prod.outlook.com> I have done the same thing with acetone instead of alcohol... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Monday, June 25, 2012 10:33 AM To: Bea DeBrosse-Serra; 'Ian R Bernard'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Muscle Biopsy Procedure One place I was at didnt get many muscle biopsies so we didnt keep liquid nitrogen and isopentane, which is what I was trained to use initially. Here's what we did when we got a muscle biopsy and it worked fine. ? After cutting the biopsy into 3-4 mm portions, we wrapped it in aluminum foil and snap froze it in a slush made from 100% alcohol and dry ice. We then shipped it in a styrofoam lined box full of peices of dry ice. The dry ice was made into small peices so we could place the snap frozen biopsy in the middle. ? Good luck! ________________________________ From: Bea DeBrosse-Serra To: 'Ian R Bernard' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, June 25, 2012 10:16 AM Subject: [Histonet] RE: Muscle Biopsy Procedure Ian, Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Friday, June 22, 2012 7:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle Biopisy Procedure Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one.? Since we never did one, we don't have a written procedure. We are looking for a? benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week.? Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Mon Jun 25 12:14:20 2012 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Mon Jun 25 12:14:19 2012 Subject: [Histonet] Thank you to all who responded Message-ID: <3E6798F00C9F494399E96B720ECD142908487F@MSGWCDCPMB22.nyumc.org> To my Microm HM 500 OM User manual request!! From galalmkh <@t> yahoo.com Mon Jun 25 13:15:52 2012 From: galalmkh <@t> yahoo.com (manal galal) Date: Mon Jun 25 13:15:56 2012 Subject: [Histonet] (no subject) Message-ID: <1340648152.15180.YahooMailNeo@web130104.mail.mud.yahoo.com> http://apartment-in-jerusalem.com/ngler.html From rsrichmond <@t> gmail.com Mon Jun 25 13:48:20 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Jun 25 13:48:23 2012 Subject: [Histonet] Re: Embedding beads Message-ID: I've never seen beads used for identifying individual embedders by marking blocks with the beads. I never heard of Hama Beads (that's how it's spelled), which I think may be called Perler Beads in the US, but I never heard of them either. I think you can get them from Amazon. Bob Richmond Samurai Pathologist Knoxville TN From aj.taylor <@t> blueyonder.co.uk Mon Jun 25 17:52:09 2012 From: aj.taylor <@t> blueyonder.co.uk (Alan Taylor) Date: Mon Jun 25 17:53:30 2012 Subject: [Histonet] Embedding beads References: <3FA597486B013249A2FC8EE113CBCC021971B36D96@BHDAEXVM33.bhcs.pvt><16ACFF4B2189457F8B050E8F35E777DC@merlin><5A33C952BB67F4468AF1F36D739212BC3082878B@JERRY.Gia.com> Message-ID: <79F573FCC6F94E63AB4E68A0E433FA39@merlin> Hi All Thanks for the correction Dr Richmond. It is Hama Beads. We keep ours in used rectangular cover slip boxes, which hold many beads of individual colours in each box. When in use each embedder has their allocated box at the back of the embedding centre, close to the dispensing spout, so that a bead can be quickly picked up with tweezers and dropped into the cassette tray when filling with wax. When not in use the boxes are kept in a small instrument tray at the side of the embedding centre. Each persons name is clearly written on the lid of each box. The stock bags are kept in a nearby drawer. Have never considered timing each embedding, but I am certain that there are very few additional seconds in reaching for a bead and dropping it into the molten wax. Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. EX1 3AJ Devon. UK. ----- Original Message ----- From: Jennifer Campbell To: Amber McKenzie Cc: Alan Taylor ; Willis, Donna G. ; histonet@lists.utsouthwestern.edu Sent: Monday, June 25, 2012 2:51 PM Subject: Re: [Histonet] Embedding beads We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab. There has to be accountability for every job. Jen Campbell On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie wrote: Does this slow the embedder down any having to pick a bead up each time to embed along with the tissue? I've never heard of this procedure before. The labs I've worked in have an embedding log and we initial each case we embed. What do you keep the beads in and are they set in a particular spot on the embedding station so they're easily assessable? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Taylor Sent: Friday, June 22, 2012 5:15 PM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Embedding beads Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make colourful place mats etc for children. Hamma beads come in a very wide range of colours, in bulk bags from any good toy or handicraft store. They are very cheap and they are clearly visible when placed in the corner of an embedding cassette when filling with wax. We have a small staff in our lab so the colours we have chosen are readily identifiable to an individual who has embedded a particular block. I can strongly recommend them, we have used them for a number of years as a traceable guide to a particular block. Hope this is helpful to all of you who are embedding lots of blocks each day. Best wishes to you all Alan Taylor BSc(Hons), FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ.UK ----- Original Message ----- From: "Willis, Donna G." To: Sent: Friday, June 22, 2012 3:58 PM Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From galinadeyneko <@t> yahoo.com Tue Jun 26 09:57:40 2012 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Jun 26 09:57:48 2012 Subject: [Histonet] macrophage stain Message-ID: <1340722660.12041.YahooMailClassic@web161003.mail.bf1.yahoo.com> I use rat-a- mouse Mac3 on FFPE mouse aortic sinus. BD pharmigen # 550292,very simple basic protocol Dilution 1;200, no need HIER with tis dilution. Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w From FUNKM <@t> mercyhealth.com Tue Jun 26 09:58:48 2012 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Jun 26 09:58:59 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org> Message-ID: <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Tue Jun 26 10:30:05 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Jun 26 10:30:12 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> References: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org> <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> Message-ID: If you will be at NSH in Vancouver, I will be presenting a workshop called "Every Step You Take: Implementing a Tracking System in the Pathology Laboratory". I presented this at the Region I meeting in NY this past April and received good feedback that the information was useful. It is scheduled for Saturday afternoon. I do concentrate on my particular lab's tracking system (while making no recommendations) but talk in depth about implementation, added values, and pros and cons. Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, June 26, 2012 10:59 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu; Loralee A McMahon Subject: [Histonet] RE: Adopting a bar code system We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Jun 26 10:39:36 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Jun 26 10:39:42 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org> <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> Message-ID: <9F3CFEE76E51B64991C7485270890B400CDBEEED@EX4.lj.gnf.org> Clare, I have already chosen this workshop and I will be attending it. Thank you for this email. Teri -----Original Message----- From: Clare Thornton [mailto:CThornton@dahlchase.com] Sent: Tuesday, June 26, 2012 8:30 AM To: 'Marcia Funk'; Teri Johnson; histonet@lists.utsouthwestern.edu; Loralee A McMahon Subject: RE: [Histonet] RE: Adopting a bar code system If you will be at NSH in Vancouver, I will be presenting a workshop called "Every Step You Take: Implementing a Tracking System in the Pathology Laboratory". I presented this at the Region I meeting in NY this past April and received good feedback that the information was useful. It is scheduled for Saturday afternoon. I do concentrate on my particular lab's tracking system (while making no recommendations) but talk in depth about implementation, added values, and pros and cons. Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, June 26, 2012 10:59 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu; Loralee A McMahon Subject: [Histonet] RE: Adopting a bar code system We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Tue Jun 26 10:49:32 2012 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue Jun 26 10:49:39 2012 Subject: [Histonet] Tissue removal from slides Message-ID: <1340725772.80693.YahooMailNeo@web161606.mail.bf1.yahoo.com> Hi Histoland, ?Does anyone know the name of the reagent that is used to remove or split tissue sections from slides? It pours on like liquid and allows you to peel off after a couple of hours, it's been years since I've done this so don't remember.. Thanks in advance!! ? Jill Cox HT (ASCP) Pathology Lab/ Histologist Cancer Treatment Centers of America Ph: 623-207-3710 F: 623-207-3717 From mamawooo <@t> hotmail.com Tue Jun 26 14:18:18 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Tue Jun 26 14:18:24 2012 Subject: [Histonet] RE: Adopting a bar code system In-Reply-To: <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> References: <9F3CFEE76E51B64991C7485270890B400CDB006D@EX5.lj.gnf.org>, , <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> Message-ID: Marcia,I'm so glad you are going with Vantage, you will not be disappointed. Good luck and call me if you need any help.Jan MahoneyOmaha Date: Tue, 26 Jun 2012 10:58:48 -0400 From: FUNKM@mercyhealth.com To: TJohnson@gnf.org; histonet@lists.utsouthwestern.edu; Loralee_Mcmahon@URMC.Rochester.edu Subject: [Histonet] RE: Adopting a bar code system CC: We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NJoshi <@t> lsuhsc.edu Tue Jun 26 14:22:06 2012 From: NJoshi <@t> lsuhsc.edu (Joshi, Neelam) Date: Tue Jun 26 14:22:44 2012 Subject: [Histonet] RE: Histonet Digest, Vol 103, Issue 31 In-Reply-To: References: Message-ID: <5C9ABB8086EAFD4CB2CBEBDFC8955080746D@SH-ExchMB2.master.lsuhsc.edu> Hello Everybody, My name is Neelam Joshi. I work for Louisiana State University Health Sciences Center. I am the supervisor of Histology and Immunohistochemistry Lab. I need the help to set the protocol to run HPV FAMILY16 low and high risk (ASR) on VENTANA XT intrument. The protocol which I have at present, having lot of background. My email address is njoshi@lsuhsc.edu amd phone# 318-675-5874 Please let me know. Thanks Neelam Joshi ,HT(ASCP) Histology Supervisor Louisiana State University Health sciences Center 1501 Kings HWY-71130 Phone# 1-318-675-5874 njoshi@lsuhsc.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, June 26, 2012 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 103, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Thank you to all who responded (Chiriboga, Luis) 2. (no subject) (manal galal) 3. Re: Embedding beads (Bob Richmond) 4. Re: Embedding beads (Alan Taylor) 5. macrophage stain (Galina Deyneko) 6. RE: Adopting a bar code system (Marcia Funk) 7. RE: RE: Adopting a bar code system (Clare Thornton) 8. RE: RE: Adopting a bar code system (Teri Johnson) 9. Tissue removal from slides (Jill Cox) ---------------------------------------------------------------------- Message: 1 Date: Mon, 25 Jun 2012 17:14:20 +0000 From: "Chiriboga, Luis" Subject: [Histonet] Thank you to all who responded To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3E6798F00C9F494399E96B720ECD142908487F@MSGWCDCPMB22.nyumc.org> Content-Type: text/plain; charset="us-ascii" To my Microm HM 500 OM User manual request!! ------------------------------ Message: 2 Date: Mon, 25 Jun 2012 11:15:52 -0700 (PDT) From: manal galal Subject: [Histonet] (no subject) To: azzamonib@yahoo.com, histonet@lists.utsouthwestern.edu, asmaa-twins@hotmail.com, almasryeg@yahoo.com Message-ID: <1340648152.15180.YahooMailNeo@web130104.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii http://apartment-in-jerusalem.com/ngler.html ------------------------------ Message: 3 Date: Mon, 25 Jun 2012 14:48:20 -0400 From: Bob Richmond Subject: [Histonet] Re: Embedding beads To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I've never seen beads used for identifying individual embedders by marking blocks with the beads. I never heard of Hama Beads (that's how it's spelled), which I think may be called Perler Beads in the US, but I never heard of them either. I think you can get them from Amazon. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Mon, 25 Jun 2012 23:52:09 +0100 From: "Alan Taylor" Subject: Re: [Histonet] Embedding beads To: "Jennifer Campbell" , "Amber McKenzie" Cc: "Willis, Donna G." , histonet@lists.utsouthwestern.edu Message-ID: <79F573FCC6F94E63AB4E68A0E433FA39@merlin> Content-Type: text/plain; charset="iso-8859-1" Hi All Thanks for the correction Dr Richmond. It is Hama Beads. We keep ours in used rectangular cover slip boxes, which hold many beads of individual colours in each box. When in use each embedder has their allocated box at the back of the embedding centre, close to the dispensing spout, so that a bead can be quickly picked up with tweezers and dropped into the cassette tray when filling with wax. When not in use the boxes are kept in a small instrument tray at the side of the embedding centre. Each persons name is clearly written on the lid of each box. The stock bags are kept in a nearby drawer. Have never considered timing each embedding, but I am certain that there are very few additional seconds in reaching for a bead and dropping it into the molten wax. Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. EX1 3AJ Devon. UK. ----- Original Message ----- From: Jennifer Campbell To: Amber McKenzie Cc: Alan Taylor ; Willis, Donna G. ; histonet@lists.utsouthwestern.edu Sent: Monday, June 25, 2012 2:51 PM Subject: Re: [Histonet] Embedding beads We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab. There has to be accountability for every job. Jen Campbell On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie wrote: Does this slow the embedder down any having to pick a bead up each time to embed along with the tissue? I've never heard of this procedure before. The labs I've worked in have an embedding log and we initial each case we embed. What do you keep the beads in and are they set in a particular spot on the embedding station so they're easily assessable? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Taylor Sent: Friday, June 22, 2012 5:15 PM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Embedding beads Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make colourful place mats etc for children. Hamma beads come in a very wide range of colours, in bulk bags from any good toy or handicraft store. They are very cheap and they are clearly visible when placed in the corner of an embedding cassette when filling with wax. We have a small staff in our lab so the colours we have chosen are readily identifiable to an individual who has embedded a particular block. I can strongly recommend them, we have used them for a number of years as a traceable guide to a particular block. Hope this is helpful to all of you who are embedding lots of blocks each day. Best wishes to you all Alan Taylor BSc(Hons), FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ.UK ----- Original Message ----- From: "Willis, Donna G." To: Sent: Friday, June 22, 2012 3:58 PM Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ Message: 5 Date: Tue, 26 Jun 2012 07:57:40 -0700 (PDT) From: Galina Deyneko Subject: [Histonet] macrophage stain To: kimulah@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1340722660.12041.YahooMailClassic@web161003.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I use rat-a- mouse Mac3 on FFPE mouse aortic sinus. BD pharmigen # 550292,very simple basic protocol Dilution 1;200, no need HIER with tis dilution. Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w ------------------------------ Message: 6 Date: Tue, 26 Jun 2012 10:58:48 -0400 From: "Marcia Funk" Subject: [Histonet] RE: Adopting a bar code system To: "Teri Johnson" , "histonet@lists.utsouthwestern.edu" , "Loralee A McMahon" Message-ID: <4FE987D8020000AC0001280F@nodcdmg2.no.trinity-health.org> Content-Type: text/plain; charset="us-ascii" We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 26 Jun 2012 11:30:05 -0400 From: Clare Thornton Subject: RE: [Histonet] RE: Adopting a bar code system To: 'Marcia Funk' , Teri Johnson , "histonet@lists.utsouthwestern.edu" , Loralee A McMahon Message-ID: Content-Type: text/plain; charset="us-ascii" If you will be at NSH in Vancouver, I will be presenting a workshop called "Every Step You Take: Implementing a Tracking System in the Pathology Laboratory". I presented this at the Region I meeting in NY this past April and received good feedback that the information was useful. It is scheduled for Saturday afternoon. I do concentrate on my particular lab's tracking system (while making no recommendations) but talk in depth about implementation, added values, and pros and cons. Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, June 26, 2012 10:59 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu; Loralee A McMahon Subject: [Histonet] RE: Adopting a bar code system We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 26 Jun 2012 15:39:36 +0000 From: Teri Johnson Subject: RE: [Histonet] RE: Adopting a bar code system To: Clare Thornton Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B400CDBEEED@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Clare, I have already chosen this workshop and I will be attending it. Thank you for this email. Teri -----Original Message----- From: Clare Thornton [mailto:CThornton@dahlchase.com] Sent: Tuesday, June 26, 2012 8:30 AM To: 'Marcia Funk'; Teri Johnson; histonet@lists.utsouthwestern.edu; Loralee A McMahon Subject: RE: [Histonet] RE: Adopting a bar code system If you will be at NSH in Vancouver, I will be presenting a workshop called "Every Step You Take: Implementing a Tracking System in the Pathology Laboratory". I presented this at the Region I meeting in NY this past April and received good feedback that the information was useful. It is scheduled for Saturday afternoon. I do concentrate on my particular lab's tracking system (while making no recommendations) but talk in depth about implementation, added values, and pros and cons. Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, June 26, 2012 10:59 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu; Loralee A McMahon Subject: [Histonet] RE: Adopting a bar code system We are installing Vantage system from Ventana and very excited - great things to follow. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "McMahon, Loralee A" 06/21/2012 1:55 PM >>> Sorry Teri must have missed the reply all button. And also Lablion claims to interface with any current lab equipment that you have. So if you already have slide printers (either etchers or labelers) it can work. Like I said. I haven't seen it in action, but I sure would like to. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Adopting a bar code system Here's a response I have received off list: Teri, We just looked at a barcode histology tracking system called LabLion. I have never heard of them until yesterday. It was developed by a histotech and some engineers. It looks too good to be true. We have looked at a lot of different systems and this ones seems to have everything that we need. We have looked at the TBS system, Dako, Ventana, etc. I think if you google them you can find it and the contact information. If not let me know and I'll get it for you. It is definitley worth your time to look at this system. We are in serious negotiations with them. But this is just my opinion from the demo. I haven't had a chance to actually put my hands on it. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Thursday, June 21, 2012 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Adopting a bar code system Hi Histonetters, I am interested in hearing from folks who went from a manual histology system to a barcoding system. I would like to look into to adopting bar coding and want to know the pitfalls, issues, and your successes in doing so. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 26 Jun 2012 08:49:32 -0700 (PDT) From: Jill Cox Subject: [Histonet] Tissue removal from slides To: "Histonet@Lists. Edu" Message-ID: <1340725772.80693.YahooMailNeo@web161606.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Histoland, ?Does anyone know the name of the reagent that is used to remove or split tissue sections from slides? It pours on like liquid and allows you to peel off after a couple of hours, it's been years since I've done this so don't remember.. Thanks in advance!! ? Jill Cox HT (ASCP) Pathology Lab/ Histologist Cancer Treatment Centers of America Ph: 623-207-3710 F: 623-207-3717 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 103, Issue 31 ***************************************** From brannon <@t> alliedsearchpartners.com Tue Jun 26 15:10:34 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Tue Jun 26 15:10:43 2012 Subject: [Histonet] HT/HTL Needed in Southwest FL Message-ID: Allied Search Partners is working with a laboratory in Sarasota, FL looking for a qualified and licensed Histotechnologist or Histotechnician. To Apply Please email or fax resume to Brannon@alliedsearchpartners.com or fax to 888 388 7572. No other information is given about location or the organization at this time. Please send resume for review by our recruiters and all qualified candidates will be submitted to HR for further review. Once we have had time to further consider your application, then more information will be made available. Thank you! Position: Histotechnician or Histotechnologist Schedule: Full time/4 day work week Location: Sarasota, FL Excellent pay and benefits! Requirements: At least 1 year working experience with Mohs and processing of permanent sections HT/HTL certification by ASCP FL Clinical Lab License Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 From foreightl <@t> gmail.com Tue Jun 26 15:11:59 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Jun 26 15:12:03 2012 Subject: [Histonet] Tissue removal from slides In-Reply-To: <1340725772.80693.YahooMailNeo@web161606.mail.bf1.yahoo.com> References: <1340725772.80693.YahooMailNeo@web161606.mail.bf1.yahoo.com> Message-ID: What we use is Mount-Quick from newcomer supply. On Tue, Jun 26, 2012 at 8:49 AM, Jill Cox wrote: > Hi Histoland, > > Does anyone know the name of the reagent that is used to remove or split > tissue sections from slides? It pours on like liquid and allows you to peel > off after a couple of hours, it's been years since I've done this so don't > remember.. Thanks in advance!! > > > Jill Cox HT (ASCP) > Pathology Lab/ Histologist > > Cancer Treatment Centers of America > Ph: 623-207-3710 F: 623-207-3717 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From kdboydhisto <@t> yahoo.com Tue Jun 26 15:20:10 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Tue Jun 26 15:20:18 2012 Subject: [Histonet] Re: Embedding beads Message-ID: <1340742010.84780.YahooMailNeo@web125802.mail.ne1.yahoo.com> I started using the embedding beads several months ago as a quick way to track who embedded what cassette. Each tech is assigned a number according to the number on their microtome. I get the beads from Cancer Diagnostics. You can probably find something similar at a craft store. These come in small round plastic containers that fit easily any where on the embedding center. They place the bead in a bottom corner of the cassette when topping it off with paraffin. They?have actually saved us time. Once the techs get used to it, it might add a few seconds to the embedding.?Before, the techs had to write down which blocks they embedded. (Very time consuming and often not complete).?If I needed to see who embedded a certain block, I had to go check that log book. Now I can see who embedded it just by looking at the block. Kelly?? From relia1 <@t> earthlink.net Tue Jun 26 15:33:02 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jun 26 15:33:21 2012 Subject: [Histonet] RELIA Hot Histology Job Alert 6-26-2012 Exciting opportunity on the Beautiful West Coast of Florida in Sarasota and Venice, FL Message-ID: <014801cd53da$e28f23e0$a7ad6ba0$@earthlink.net> Hi Histonetters! I hope everybody is having a great day. I just got off the phone with one of my favorite clients located in Sarasota, FL and have a really great opportunity to run by you. My client is looking for an ASCP HT/HTL certified and Florida licensed histo tech with Mohs experience. The pay is great the bennies are excellent. We are talking 4 day work weeks and lots of time for beachy fun. If you think you might be interested or know someone who might be interested please contact me right away. You can reach me via email at relia1@earthlink.net or toll free at 866-607-3542. Remember if I place someone that you refer to me RELIA will pay you a referral fee. Thanks-Pam Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From kc <@t> ka-recruiting.com Tue Jun 26 16:53:04 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Tue Jun 26 16:53:05 2012 Subject: [Histonet] High complexity Histology job Message-ID: <1770472130.1340747584399.JavaMail.cfservice@sl4app3> Dear Histonet Community, I am a one of the founders of a nationally recognized healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in taking the next step in your career. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position in Orange County, CA Unique Histology Opportunity in So. California This high complexity lab is looking to hire a full time Histotechnologist to work a 2nd shift. This lab is only one of a handful in the country that deals exclusively with very rare samples. This unique work environment allows techs to get valuable work experience that will grow their skill set immensely. Qualifications include an Associates or Bachelor?s Degree in Histology and HT or HTL (ASCP) certification. This lab offers one of the better compensation and benefits package around. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com To learn about additional opportunities please contact me at kc@ka-recruiting.com. I look forward to hearing from you. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 27 09:44:43 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 27 09:44:51 2012 Subject: [Histonet] DEEPER REQUESTS Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4301A344B27D2D@HPEMX3.HealthPartners.int> Does anyone know if there is an average percentage that a histology lab should expect in deeper/recut requests? I am trying to establish if our pathologists order more than the "norm" or if I have a quality issue unfolding!! Also, do most labs use 2 controls for their "pneumo" staining, a routine fungus and a pneumo carnii positive? Thanks ahead of time! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From trathborne <@t> somerset-healthcare.com Wed Jun 27 10:01:43 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jun 27 09:59:13 2012 Subject: [Histonet] RE: DEEPER REQUESTS In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4301A344B27D2D@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4301A344B27D2D@HPEMX3.HealthPartners.int> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757071298B0BD@smcmail02.somerset-healthcare.com> For the biopsies that routinely have levels taken, our pathologists would prefer to order deepers rather than have a possibly diagnostic piece of the tissue trimmed away before obtaining a section. Yours may feel the same way. With regard to the controls, our pathologists will specify which control they want to see at the time of the stain request, so we do not use both together. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, June 27, 2012 10:45 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DEEPER REQUESTS Does anyone know if there is an average percentage that a histology lab should expect in deeper/recut requests? I am trying to establish if our pathologists order more than the "norm" or if I have a quality issue unfolding!! Also, do most labs use 2 controls for their "pneumo" staining, a routine fungus and a pneumo carnii positive? Thanks ahead of time! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From twheelock <@t> mclean.harvard.edu Wed Jun 27 09:59:18 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jun 27 09:59:23 2012 Subject: [Histonet] Pens for writing cassettes Message-ID: <4FEB1FC6.1050007@mclean.harvard.edu> Hi Everyone: I want to thank everyone who responded to my inquiry regarding marking pens a while ago. I had a problem with the Securline Marker2/Superfrost pens fading from my tissue cassettes. All three of the pens that I tried: StatLab, Leica, and the Histotec brands worked really well. There was no fading on the tissue cassettes at all. The writing was as clear and crisp as when they were first written. I also can still use the Securline Marker 2 pens for writing out my slides. Thanks again, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From rjbuesa <@t> yahoo.com Wed Jun 27 10:03:47 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 27 10:03:56 2012 Subject: [Histonet] DEEPER REQUESTS In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4301A344B27D2D@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4301A344B27D2D@HPEMX3.HealthPartners.int> Message-ID: <1340809427.53555.YahooMailNeo@web121405.mail.ne1.yahoo.com> That could be a very difficult to solve?issue in some labs. It is entirely dependent on the pathologists and how confident they?feel about their individual diagnostic abilities and the complexity of some cases. Some pathologists request more deepers than others and you cannot use the "norm" of other labs to yours. What are you going to say to a pathologist in your lab that YOU think is requesting too many deepers? S/he will dismiss your concern and maybe will feel even "insulted". As to your concern that you may have a quality issue as the cause of the problem, fear not. Generally speaking pathologists are not very good at praising your good work, but rest assured that if it was a quality issue you would have had an ear full of complaints by now. As to the 2 controls I never used them for Pneumocystis carinii, but only to PAS with/without diastase. Ren? J. ________________________________ From: "Webb, Dorothy L" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, June 27, 2012 10:44 AM Subject: [Histonet] DEEPER REQUESTS Does anyone know if there is an average percentage that a histology lab should expect in deeper/recut requests?? I am trying to establish if our pathologists order more than the "norm" or if I have a quality issue unfolding!! Also, do most labs use 2 controls for their "pneumo" staining, a routine fungus and a pneumo carnii positive? Thanks ahead of time! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Wed Jun 27 10:09:32 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jun 27 10:09:44 2012 Subject: [Histonet] Luxol Fast Blue problem Message-ID: <4FEB222C.2030700@mclean.harvard.edu> Hi again all: Lately, I have had problems with my Luxol Fast Blue myelin stain not being nearly as dark as it should. I am having my oven checked out because I have had problems with it holding a high enough temperature (at least 60C). This seems to one factor in getting the stain to take. But I suspect there may be other factors involved. Has anyone had this sort of problem before? What did you find was the cause and how did you fix the problem? Thanks for any advice you can provide. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From rjbuesa <@t> yahoo.com Wed Jun 27 10:19:22 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 27 10:19:26 2012 Subject: [Histonet] Luxol Fast Blue problem In-Reply-To: <4FEB222C.2030700@mclean.harvard.edu> References: <4FEB222C.2030700@mclean.harvard.edu> Message-ID: <1340810362.49663.YahooMailNeo@web121406.mail.ne1.yahoo.com> The only thing I think now refers to the "age" of your solution. If you prepare it yourself or if bought and near the "expiration date" probably that could be another cause of the problem. Ren? J. ________________________________ From: Tim Wheelock To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, June 27, 2012 11:09 AM Subject: [Histonet] Luxol Fast Blue problem Hi again all: Lately, I have had problems with my Luxol Fast Blue myelin stain not being nearly as dark as it should. I am having my oven checked out? because? I have had problems with? it holding a? high enough temperature (at least 60C). This seems to one factor in getting the stain to take. But I suspect there may be other factors involved. Has anyone had this sort of problem before? What did you find was the cause and how did you fix the problem? Thanks for any advice you can provide. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Wed Jun 27 11:07:43 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Jun 27 11:07:49 2012 Subject: [Histonet] fume hoods Message-ID: <1340813263.610.YahooMailNeo@web125801.mail.ne1.yahoo.com> Can anyone tell me a company that will test airflow velocity of fume hoods? Close to or in NC. Kelly?? From algranth <@t> email.arizona.edu Wed Jun 27 11:30:19 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jun 27 11:30:31 2012 Subject: [Histonet] Luxol Fast Blue problem In-Reply-To: <1340810362.49663.YahooMailNeo@web121406.mail.ne1.yahoo.com> References: <4FEB222C.2030700@mclean.harvard.edu> <1340810362.49663.YahooMailNeo@web121406.mail.ne1.yahoo.com> Message-ID: <67905F9B-559F-461F-9CBB-F7EA4C6AD6FF@email.arizona.edu> True, if you solution is old it may not give the blue color that you want. Are you doing the overnite procedure? I have found this to be the best. If your oven isn't holding at 60? C you could use a waterbath. Andi G Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 ? algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From patrick.lewis <@t> seattlechildrens.org Wed Jun 27 11:45:26 2012 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Jun 27 11:45:38 2012 Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates Message-ID: <3903BE18914F4440834F0E620415FFCA0BCC384C@PPWEXD01B.childrens.sea.kids> Hi everyone, I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. I love DAKO, but their kits and reagents are expensive. Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. Thanks Patrick. PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. Has anyone tried Spring Bioscience's products? CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From relia1 <@t> earthlink.net Wed Jun 27 11:53:07 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jun 27 11:53:31 2012 Subject: [Histonet] RELIA HOT Histology Job Alert - 6-27-2012 Message-ID: <012a01cd5485$53fc4e40$fbf4eac0$@earthlink.net> Hi Histonetters!! How are you doing today? Are you gearing up for a fun 4th of July or getting ready for your well- deserved summer vacation? I wanted to drop you a quick line to tell you about some especially great opportunities that I am working on. So grab a cool drink and find some shade and check this out. I am especially excited about these openings because they are with several of my best clients. Why do I refer to them as best clients . Well for a variety of reasons: * Location, * The rest of the team, * The fantastic manager, * Excellent benefits, * Generous relocation * And most importantly I like a client that only has openings due to growth. Here is a list of the positions that I am most excited about.: Histo Tech with Mohs - Sarasota, FL IHC Tech - Long Island, NY Electron Microscopy Tech - Long Island, NY HT/HTL with IHC - Philadelphia Molecular Technologist - Nashville, TN Histology Tech - Louisville, KY Histotech - Portland, ME Mohs Tech - Irving, TX Histotech ASCP or ELIG - Lafayette, LA HT Histotechnican - Kingman, AZ Lead Histotech - Columbus, OH If you or anyone you know is interested in more details I can be reached toll free at 866-607-3542 or relia1@earthlink.net. Thanks and have a great day!! >Pam Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 27 12:00:40 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jun 27 12:00:23 2012 Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates In-Reply-To: <3903BE18914F4440834F0E620415FFCA0BCC384C@PPWEXD01B.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA0BCC384C@PPWEXD01B.childrens.sea.kids> Message-ID: <8D7C2D242DBD45498006B21122072BF8B59311CB@MCINFRWEM003.ucsfmedicalcenter.org> Patrick, Vector, Lab Vision (Thermo), Jackson Labs, Scytek Labs, all have much less expensive reagents than Dako. Scytek Labs supplies re-badged reagents for many, many companies and has a large selection of reagents. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, June 27, 2012 9:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates Hi everyone, I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. I love DAKO, but their kits and reagents are expensive. Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. Thanks Patrick. PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. Has anyone tried Spring Bioscience's products? CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Wed Jun 27 12:09:51 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Wed Jun 27 12:09:56 2012 Subject: [Histonet] Recommendations for HRP anti Rabbit and mouseand AEC substrates Message-ID: <2fcb909b661a7ec89a1c93ca82cdc9d4@medicalcenterclinic.com> Biocare is another alternative as well. Brendal C. Finlay, HT (ASCP) West Florida Medical Center Clinic brendal.finlay@medicalcenterclinic.com -----Original message----- From: "Morken, Timothy" Timothy.Morken@ucsfmedctr.org Date: Wed, 27 Jun 2012 11:00:40 -0500 To: "Lewis, Patrick" patrick.lewis@seattlechildrens.org, "'Histonet@lists.utsouthwestern.edu'" Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recommendations for HRP anti Rabbit and mouseand AEC substrates > Patrick, > > Vector, Lab Vision (Thermo), Jackson Labs, Scytek Labs, all have much less expensive reagents than Dako. Scytek Labs supplies re-badged reagents for many, many companies and has a large selection of reagents. > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular SpecialStudies > Department of Pathology > UC San Francisco Medical Center > tim.morken@ucsfmedctr.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick > Sent: Wednesday, June 27, 2012 9:45 AM > To: 'Histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates > > Hi everyone, > > I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. > > I love DAKO, but their kits and reagents are expensive. > > Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. > > I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of whichare large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. > > Thanks > > Patrick. > > PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. > Has anyone tried Spring Bioscience's products? > > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ronald.Houston <@t> nationwidechildrens.org Wed Jun 27 12:17:36 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jun 27 12:17:49 2012 Subject: [Histonet] Luxol Fast Blue problem In-Reply-To: <4FEB222C.2030700@mclean.harvard.edu> References: <4FEB222C.2030700@mclean.harvard.edu> Message-ID: Luxol fast blue staining is dependent on the polarity of the solvent. Weak staining can occur if the solvent becomes too polar, i.e. if sections are taken down to water rather than 95% alcohol prior to putting in the staining solution. Also check the amount of water in the ethanol or methanol used to make up the LFB Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, June 27, 2012 11:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Luxol Fast Blue problem Hi again all: Lately, I have had problems with my Luxol Fast Blue myelin stain not being nearly as dark as it should. I am having my oven checked out because I have had problems with it holding a high enough temperature (at least 60C). This seems to one factor in getting the stain to take. But I suspect there may be other factors involved. Has anyone had this sort of problem before? What did you find was the cause and how did you fix the problem? Thanks for any advice you can provide. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From BDeBrosse-Serra <@t> isisph.com Wed Jun 27 12:37:57 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Jun 27 12:38:06 2012 Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates In-Reply-To: <3903BE18914F4440834F0E620415FFCA0BCC384C@PPWEXD01B.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA0BCC384C@PPWEXD01B.childrens.sea.kids> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092A969BD4@EXCHMB01.isis.local> We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, June 27, 2012 9:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates Hi everyone, I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. I love DAKO, but their kits and reagents are expensive. Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. Thanks Patrick. PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. Has anyone tried Spring Bioscience's products? CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Wed Jun 27 13:23:26 2012 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Jun 27 13:23:40 2012 Subject: [Histonet] Deeper Requests (and P.Carinii Controls) Message-ID: <5F06C3AD0B27264CA20CFA986C87882E314B50B4@EXCHANGEPV3.KGH.ON.CA> Hi Dorothy, I completely agree with Rene's response regarding the issue of deepers. We have had this question raised by the number of requests from a few pathologists over the years, and the 'norm' would depend on many factors i.e. case mix, nature of tissue, pathologist need, and yes, possible embedding issues. Regarding the fungus controls, the best control to judge endpoint is P.Carinii positive case. However, if possible, I like to have a regular fungus control along for the ride, to judge the silver methenamine solution and when the endpoint may be approaching. This is personal preference on my part, and our procedure requires only a P. Carinii control. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From rsrichmond <@t> gmail.com Wed Jun 27 14:56:26 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jun 27 14:56:29 2012 Subject: [Histonet] Re: DEEPER REQUESTS Message-ID: When I request deeper cuts of a paraffin block, I'm usually looking for a focal lesion that didn't show up in the first section. I don't usually order a recut because of a crappy slide, knowing that what I'll get is another crappy slide. I'd actually prefer to write a very brief note explaining what I'm looking for. Since I usually want to examine the block before asking for the recut, I tell the technologist then. Thus cervix - can't find the dysplasia skin - not sure we're down to the lesion in question breast - looking for calcifications The fixable quality issue I'd be thinking of is - am I providing the pathologist with enough sections? (More than one slide, more than one ribbon on one slide.) One short ribbon on one slide isn't always enough. I've never encountered the problem of a pathologist ordering too many deepers, but it isn't a problem that another pathologist in the practice would be likely to notice. Bob Richmond Samurai Pathologist Knoxville TN From cebass <@t> buffalo.edu Wed Jun 27 16:27:41 2012 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Wed Jun 27 16:27:46 2012 Subject: [Histonet] AO860 repair advice Message-ID: <8CE693FA-41B4-47A8-A11B-8F63DC3A3EC2@buffalo.edu> Hi Guys, I'm taking apart my AO860 and have a couple issues. First is trying to pull apart the central shaft that the microtome lowers and raises on. I can get the primary parts away from each other, and pull off the gears associated with the handle, but the gear on the central shaft is a problem. Normally I would just ignore it but there is some gunk in there that is definitely catching. I'll try soaking in mineral spirits, but if that doesn't work I'd like to take the shaft off. The three units are conspiring against me. It looks like one pin, running perpendicularly through the shaft is holding it all together, but I don't know for sure. There is a top plate that prevents the bottom plate from coming off and then the gear prevents the bottom plate from coming off. I can move everything about 1 cm so hopefully the solvent can get in there. I'm just perplexed at this particular part. Should I be able to take it apart? Am I missing something? Here are some pictures? the middle one shows the top plate, bottom plate and pin in the shaft. The bottom picture shows the clearance I have in moving them all together. http://imgur.com/a/DkF3j The second thing is one screw is stripped and won't come off. This is a flathead small screw. It's not totally stripped, but it's stopped up enough that there's no way brute force can do it. Any advice? I've thought of supergluing the screw to the screwdriver, but I don't want to wreck my nice microtome! Thanks in advance, Caroline Bass University at Buffalo From norogers <@t> maxhealth.com Thu Jun 28 08:09:06 2012 From: norogers <@t> maxhealth.com (Noah Rogers) Date: Thu Jun 28 08:09:29 2012 Subject: [Histonet] Senior Histotechnologist Position - Washington, DC (NW) Message-ID: Hello All: There is a Full-Time, Permanent, Contract-to-Hire position available as a Senior HistoTech (HTL preferred, not mandatory) at a strong Hospital facility in NW Washington, DC. I would love to go into detail with any interested/qualified individuals with regard to this opportunity. If you would like to learn more, or have your Resume/CV submitted for the opportunity - please reply to this email with an updated version of your resume, or give me a buzz at one of the listings below (Email correspondence is just fine, as well). Thank you SO much Histonet! First time user, pleasure to work with you all. Have a great day, Noah Noah Rogers Sr. Recruiter | Maxim Healthcare Services DC/MD/VA - Allied Staffing Division 9525 Georgia Ave | Suite 205 Silver Spring, MD 20910 301.755.2089 (P) 877.748.8793 (F) 703.597.9186 (C) Confidentiality Statement: "The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction." Confidential Health Information Attached. "Health care information is personal and sensitive. It is being faxed/emailed to you after appropriate authorization from the patient/employee or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain this information in a safe, secure and confidential manner. Re-disclosure without additional patient consent or authorization or as permitted by law is prohibited. Unauthorized re-disclosure or failure to maintain the confidentiality of this information could subject you to penalties under Federal and/or State law." Thank you. ________________________________ Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. From dreynold <@t> mdanderson.org Thu Jun 28 09:27:13 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Thu Jun 28 09:26:34 2012 Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and Message-ID: <785BBF0C5F49CE41BA74460A43A08F023219DDC2D4@DCPWVMBXC0VS3.mdanderson.edu> I am not sure what all is in the kits. We rarely buy kits, they are expensive and often as not you can get better reagents by buying them individually. For our HRP-IHC we buy HRP conjugated antibodies from Jackson Immuno. They have a large selection, reasonably priced, and work great. For AEC substrate take a look at BioCare Medical. They offer several different kits and colors. We have used the Stable DAB for decades, it was developed by Dr. Brigatti . It has sold several times to different companies. It was recently bought by Invitrogen and so far they are still selling. It is a great DAB. We have slides stained in the 80's that have not faded. No mixing ready to use solution. I just hope Invitrogen doesn't discontinue it. Donna Reynolds, ASCP, Chief Histology Laboratory Specializing in research immuno labeling U.T. M.D. Anderson Cancer Center Houston, Texas 713-792-8106 From boneimage8 <@t> gmail.com Thu Jun 28 10:36:16 2012 From: boneimage8 <@t> gmail.com (Marc DeCarlo) Date: Thu Jun 28 10:36:24 2012 Subject: [Histonet] Staing rack for ground section?? Message-ID: Hello Histoland! I've recently been tasked with doing some ground section work and can not find a staining rack suited specifically for 50 x 100mm slides. Does anybody know where I can find one? Thanks, Marc DeCarlo From b427297 <@t> aol.com Thu Jun 28 10:40:26 2012 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Jun 28 10:40:51 2012 Subject: [Histonet] Gross macro digital camera In-Reply-To: References: Message-ID: <17A905B6-1769-40CD-8F1A-467A5C78047C@aol.com> Would appreciate suggestions for a good digital camera for taking macro photos during necropsy. Any takers? Thanks much. Jackie O' From ratliffjack <@t> hotmail.com Thu Jun 28 10:44:14 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jun 28 10:44:22 2012 Subject: [Histonet] Staing rack for ground section?? In-Reply-To: References: Message-ID: Marc, May I ask what staining protocol you are using, typical section thickness and what type of specimens you are staining? The problem is that no such product exists and most likely because some believe that you cannot batch stain 50 x 100mm EXAKT slides due to the complexity of some staining protocols, tissue types, and lack of true consistency from a section thickness perspective. I could help steer you in the right direction, but I will need to know more about what you are working with in order to provide the best advice. Best Regards, Jack Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology > Date: Thu, 28 Jun 2012 10:36:16 -0500 > From: boneimage8@gmail.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Staing rack for ground section?? > > Hello Histoland! > > I've recently been tasked with doing some ground section work and can not > find a staining rack suited specifically for 50 x 100mm slides. Does > anybody know where I can find one? > > Thanks, > > Marc DeCarlo > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Thu Jun 28 11:00:07 2012 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Jun 28 11:00:15 2012 Subject: [Histonet] VoiceBrook Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4174054373@EXCHANGESB> Is anyone using VoiceBrook voice recognition? If so are you using it at grossing? Does it come with a scanner to bring up the case or does it bring up the case using the microphones? Thanks for any info. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From b-frederick <@t> northwestern.edu Thu Jun 28 11:15:03 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 28 11:15:14 2012 Subject: [Histonet] A poem Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> I've had this for years- don't know where I got it but thought you all might be amused. "LAB HIERARCHY" Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says "look at the choo-choo" Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God - Anonymous Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From LSebree <@t> uwhealth.org Thu Jun 28 11:20:13 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jun 28 11:20:21 2012 Subject: [Histonet] A poem In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> Message-ID: I LOVE this Bernice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, June 28, 2012 11:15 AM To: Fellow HistoNetters Subject: [Histonet] A poem I've had this for years- don't know where I got it but thought you all might be amused. "LAB HIERARCHY" Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says "look at the choo-choo" Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God - Anonymous Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Thu Jun 28 11:52:47 2012 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Jun 28 11:53:05 2012 Subject: [Histonet] A poem In-Reply-To: References: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> Message-ID: I have this poem and I got it at one of the NSH meetings from Triangle Biomedical Sciences but no author. Roberta Horner HT/HTL Penn State University From Petticoffer.Andrew <@t> synthes.com Thu Jun 28 12:12:34 2012 From: Petticoffer.Andrew <@t> synthes.com (Petticoffer.Andrew@synthes.com) Date: Thu Jun 28 12:14:56 2012 Subject: [Histonet] Staing rack for ground section?? In-Reply-To: References: Message-ID: Check out the link below for BRL. The slides aren't a perfect fit but will stay in place if you're careful. I usually space the slides every other in case of any movement, but have done more with good results. It's the best things I've found to date for that size slide and has been a real time saver. http://brainresearchlab.com/online-catalog/steel-staining-rack-30-slides -4/ Andy Andrew Petticoffer Research Associate DePuy Synthes - Biomaterials 1230 Wilson Drive West Chester, PA 19380 Phone: (484) 356-9576 Fax: (484) 356-9591 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marc DeCarlo Sent: Thursday, June 28, 2012 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Staing rack for ground section?? Hello Histoland! I've recently been tasked with doing some ground section work and can not find a staining rack suited specifically for 50 x 100mm slides. Does anybody know where I can find one? Thanks, Marc DeCarlo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epeters2 <@t> gmu.edu Thu Jun 28 12:18:16 2012 From: epeters2 <@t> gmu.edu (Esther Peters) Date: Thu Jun 28 12:18:22 2012 Subject: [Histonet] A poem In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> Message-ID: <4FEC91D8.8090705@gmu.edu> I got a copy of that from my mentor, Paul Yevich, Chief Histopathologist at the USEPA Environmental Research Laboratory, some 30ish years ago, which I keep posted in my lab. I'm glad it will now be preserved forever on the Internet so we can all get a fresh copy when the paper crumbles! Esther -- Esther C. Peters, Ph.D. Department of Environmental Science& Policy Biology Program, Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 E-mail: epeters2@gmu.edu On 6/28/2012 12:15 PM, Bernice Frederick wrote: > I've had this for years- don't know where I got it but thought you all might be amused. > > "LAB HIERARCHY" > > Chief Pathologist > Leaps tall building in a single bound > Is more powerful than a locomotive > Is faster than a speeding bullet > Walks on water, > Gives policy to God > > Associate Pathologist > Leaps short buildings in single bound > Is more powerful than a switch engine > Is just as fast as a speeding bullet > Walks on water if sea is calm > Talks with God > > The Department Head > Leaps short buildings with a running start and favorable winds > Is almost as powerful as a switch engine > Is faster than a speeding bullet > Walks on water in an indoor pool > Talks with God if special request is approved > > Director of Laboratories > Rarely clears a Quonset hut > Loses tug of war with locomotive > Can fire a speeding bullet > Swims well > Is occasionally addressed by God > > Associate Director of Laboratories > Makes high marks on the walls when trying to leap tall buildings > Is run over by locomotives > Can sometimes handle a gun without inflicting self-injury > Dog paddles > Talks to animals > > Supervisor > Runs into building > Recognizes locomotives two out of three times > Is not issued ammunition > Can stay afloat with a life jacket > Talks to walls > > Chief Technologist > Falls over doorstep when trying to enter building > Says "look at the choo-choo" > Wets themselves with a water pistol > Plays in mud puddles > Mumbles to Themselves > > Histotechnologist > Lifts tall buildings and walks under them > Kicks locomotives off the tracks > Catches speeding bullets in their teeth and eats them > Freezes water with a single glance > They are God > > > - Anonymous > > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgill <@t> marylandgeneral.org Thu Jun 28 12:38:29 2012 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Jun 28 12:38:37 2012 Subject: [Histonet] A poem In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF1E267A2E@evcspmbx3.ads.northwestern.edu> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23AA042@MDGEN-EXCH1.marylandgeneral.org> LOVE IT :D -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, June 28, 2012 12:15 PM To: Fellow HistoNetters Subject: [Histonet] A poem I've had this for years- don't know where I got it but thought you all might be amused. "LAB HIERARCHY" Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says "look at the choo-choo" Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God - Anonymous Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Petticoffer.Andrew <@t> synthes.com Thu Jun 28 13:50:53 2012 From: Petticoffer.Andrew <@t> synthes.com (Petticoffer.Andrew@synthes.com) Date: Thu Jun 28 13:51:55 2012 Subject: [Histonet] Staing rack for ground section?? In-Reply-To: References: Message-ID: Sorry the link got cut off. To get to the correct slide rack, search catalog #3004 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Petticoffer.Andrew@synthes.com Sent: Thursday, June 28, 2012 1:13 PM To: boneimage8@gmail.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Staing rack for ground section?? Check out the link below for BRL. The slides aren't a perfect fit but will stay in place if you're careful. I usually space the slides every other in case of any movement, but have done more with good results. It's the best things I've found to date for that size slide and has been a real time saver. http://brainresearchlab.com/online-catalog/steel-staining-rack-30-slides -4/ Andy Andrew Petticoffer Research Associate DePuy Synthes - Biomaterials 1230 Wilson Drive West Chester, PA 19380 Phone: (484) 356-9576 Fax: (484) 356-9591 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marc DeCarlo Sent: Thursday, June 28, 2012 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Staing rack for ground section?? Hello Histoland! I've recently been tasked with doing some ground section work and can not find a staining rack suited specifically for 50 x 100mm slides. Does anybody know where I can find one? Thanks, Marc DeCarlo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boneimage8 <@t> gmail.com Thu Jun 28 15:54:27 2012 From: boneimage8 <@t> gmail.com (Marc DeCarlo) Date: Thu Jun 28 15:54:33 2012 Subject: [Histonet] Re: Staing rack for ground section?? In-Reply-To: References: Message-ID: Thanks for all the advice and help from those that responded. Your knowledge is greatly appreciated. @ Andy, you mentioned the slides don't quite fit; is it the length or height that doesn't fit? Thanks in advance, Marc D On Thursday, June 28, 2012, Petticoffer.Andrew@synthes.com wrote: > Sorry the link got cut off. > To get to the correct slide rack, search catalog #3004 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > Behalf Of > Petticoffer.Andrew@synthes.com > Sent: Thursday, June 28, 2012 1:13 PM > To: boneimage8@gmail.com ; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Staing rack for ground section?? > > Check out the link below for BRL. The slides aren't a perfect fit but > will stay in place if you're careful. I usually space the slides every > other in case of any movement, but have done more with good results. > It's the best things I've found to date for that size slide and has been > a real time saver. > > > http://brainresearchlab.com/online-catalog/steel-staining-rack-30-slides > -4/ > > Andy > > Andrew Petticoffer > Research Associate > DePuy Synthes - Biomaterials > 1230 Wilson Drive > West Chester, PA 19380 > Phone: (484) 356-9576 > Fax: (484) 356-9591 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > Behalf Of Marc > DeCarlo > Sent: Thursday, June 28, 2012 11:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staing rack for ground section?? > > Hello Histoland! > > I've recently been tasked with doing some ground section work and can > not find a staining rack suited specifically for 50 x 100mm slides. > Does anybody know where I can find one? > > Thanks, > > Marc DeCarlo > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Thu Jun 28 17:36:02 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jun 28 17:36:08 2012 Subject: [Histonet] Re: Staing rack for ground section?? In-Reply-To: References: , , , Message-ID: Marc, My apologies for butting in here, but basically the length of the rack is a bit larger than the 100 mm slides and a looser fit. Also, the height of the slides is larger than the rack inserts and can sometimes create a stability problem when staining a full rack. As Andrew has stated, it is the next best thing and this product can work for you. However, the staining dish is also a problem because the slides do not stain vertical in the traditional sense meaning that you need the larger 600 ml volume dishes. If you would like to contact me privately, I would love to speak with you more about what you are trying to accomplish and help point you in the right direction with this sort of thing. Best Regards, Jack > Date: Thu, 28 Jun 2012 15:54:27 -0500 > From: boneimage8@gmail.com > To: Petticoffer.Andrew@synthes.com > CC: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Staing rack for ground section?? > > Thanks for all the advice and help from those that responded. Your > knowledge is greatly appreciated. > > @ Andy, you mentioned the slides don't quite fit; is it the length or > height that doesn't fit? > > Thanks in advance, > > Marc D > > On Thursday, June 28, 2012, Petticoffer.Andrew@synthes.com wrote: > > > Sorry the link got cut off. > > To get to the correct slide rack, search catalog #3004 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > > Behalf Of > > Petticoffer.Andrew@synthes.com > > Sent: Thursday, June 28, 2012 1:13 PM > > To: boneimage8@gmail.com ; Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Staing rack for ground section?? > > > > Check out the link below for BRL. The slides aren't a perfect fit but > > will stay in place if you're careful. I usually space the slides every > > other in case of any movement, but have done more with good results. > > It's the best things I've found to date for that size slide and has been > > a real time saver. > > > > > > http://brainresearchlab.com/online-catalog/steel-staining-rack-30-slides > > -4/ > > > > Andy > > > > Andrew Petticoffer > > Research Associate > > DePuy Synthes - Biomaterials > > 1230 Wilson Drive > > West Chester, PA 19380 > > Phone: (484) 356-9576 > > Fax: (484) 356-9591 > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > > Behalf Of Marc > > DeCarlo > > Sent: Thursday, June 28, 2012 11:36 AM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Staing rack for ground section?? > > > > Hello Histoland! > > > > I've recently been tasked with doing some ground section work and can > > not find a staining rack suited specifically for 50 x 100mm slides. > > Does anybody know where I can find one? > > > > Thanks, > > > > Marc DeCarlo > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsjurczak <@t> comcast.net Thu Jun 28 18:19:52 2012 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Thu Jun 28 18:20:05 2012 Subject: [Histonet] Re: Staing rack for ground section?? In-Reply-To: Message-ID: <700251915.587347.1340925592891.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> How many slides do you want it to hold? ----- Original Message ----- From: "Jack Ratliff" To: "Marc" , "petticoffer andrew" Cc: "Histonet" Sent: Thursday, June 28, 2012 5:36:02 PM Subject: RE: [Histonet] Re: Staing rack for ground section?? Marc, My apologies for butting in here, but basically the length of the rack is a bit larger than the 100 mm slides and a looser fit. Also, the height of the slides is larger than the rack inserts and can sometimes create a stability problem when staining a full rack. As Andrew has stated, it is the next best thing and this product can work for you. However, the staining dish is also a problem because the slides do not stain vertical in the traditional sense meaning that you need the larger 600 ml volume dishes. If you would like to contact me privately, I would love to speak with you more about what you are trying to accomplish and help point you in the right direction with this sort of thing. Best Regards, Jack > Date: Thu, 28 Jun 2012 15:54:27 -0500 > From: boneimage8@gmail.com > To: Petticoffer.Andrew@synthes.com > CC: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Staing rack for ground section?? > > Thanks for all the advice and help from those that responded. Your > knowledge is greatly appreciated. > > @ Andy, you mentioned the slides don't quite fit; is it the length or > height that doesn't fit? > > Thanks in advance, > > Marc D > > On Thursday, June 28, 2012, Petticoffer.Andrew@synthes.com wrote: > > > Sorry the link got cut off. > > To get to the correct slide rack, search catalog #3004 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > > Behalf Of > > Petticoffer.Andrew@synthes.com > > Sent: Thursday, June 28, 2012 1:13 PM > > To: boneimage8@gmail.com ; Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Staing rack for ground section?? > > > > Check out the link below for BRL. The slides aren't a perfect fit but > > will stay in place if you're careful. I usually space the slides every > > other in case of any movement, but have done more with good results. > > It's the best things I've found to date for that size slide and has been > > a real time saver. > > > > > > http://brainresearchlab.com/online-catalog/steel-staining-rack-30-slides > > -4/ > > > > Andy > > > > Andrew Petticoffer > > Research Associate > > DePuy Synthes - Biomaterials > > 1230 Wilson Drive > > West Chester, PA 19380 > > Phone: (484) 356-9576 > > Fax: (484) 356-9591 > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > > Behalf Of Marc > > DeCarlo > > Sent: Thursday, June 28, 2012 11:36 AM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Staing rack for ground section?? > > > > Hello Histoland! > > > > I've recently been tasked with doing some ground section work and can > > not find a staining rack suited specifically for 50 x 100mm slides. > > Does anybody know where I can find one? > > > > Thanks, > > > > Marc DeCarlo > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsjurczak <@t> comcast.net Thu Jun 28 18:20:37 2012 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Thu Jun 28 18:20:51 2012 Subject: [Histonet] Re: Staing rack for ground section?? In-Reply-To: Message-ID: <39215451.587382.1340925637250.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> There is a quick and dirty way to do this if you don't need a lot of slides at once. ----- Original Message ----- From: "Jack Ratliff" To: "Marc" , "petticoffer andrew" Cc: "Histonet" Sent: Thursday, June 28, 2012 5:36:02 PM Subject: RE: [Histonet] Re: Staing rack for ground section?? Marc, My apologies for butting in here, but basically the length of the rack is a bit larger than the 100 mm slides and a looser fit. Also, the height of the slides is larger than the rack inserts and can sometimes create a stability problem when staining a full rack. As Andrew has stated, it is the next best thing and this product can work for you. However, the staining dish is also a problem because the slides do not stain vertical in the traditional sense meaning that you need the larger 600 ml volume dishes. If you would like to contact me privately, I would love to speak with you more about what you are trying to accomplish and help point you in the right direction with this sort of thing. Best Regards, Jack > Date: Thu, 28 Jun 2012 15:54:27 -0500 > From: boneimage8@gmail.com > To: Petticoffer.Andrew@synthes.com > CC: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Staing rack for ground section?? > > Thanks for all the advice and help from those that responded. Your > knowledge is greatly appreciated. > > @ Andy, you mentioned the slides don't quite fit; is it the length or > height that doesn't fit? > > Thanks in advance, > > Marc D > > On Thursday, June 28, 2012, Petticoffer.Andrew@synthes.com wrote: > > > Sorry the link got cut off. > > To get to the correct slide rack, search catalog #3004 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > > Behalf Of > > Petticoffer.Andrew@synthes.com > > Sent: Thursday, June 28, 2012 1:13 PM > > To: boneimage8@gmail.com ; Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Staing rack for ground section?? > > > > Check out the link below for BRL. The slides aren't a perfect fit but > > will stay in place if you're careful. I usually space the slides every > > other in case of any movement, but have done more with good results. > > It's the best things I've found to date for that size slide and has been > > a real time saver. > > > > > > http://brainresearchlab.com/online-catalog/steel-staining-rack-30-slides > > -4/ > > > > Andy > > > > Andrew Petticoffer > > Research Associate > > DePuy Synthes - Biomaterials > > 1230 Wilson Drive > > West Chester, PA 19380 > > Phone: (484) 356-9576 > > Fax: (484) 356-9591 > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On > > Behalf Of Marc > > DeCarlo > > Sent: Thursday, June 28, 2012 11:36 AM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Staing rack for ground section?? > > > > Hello Histoland! > > > > I've recently been tasked with doing some ground section work and can > > not find a staining rack suited specifically for 50 x 100mm slides. > > Does anybody know where I can find one? > > > > Thanks, > > > > Marc DeCarlo > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> texasheart.org Fri Jun 29 07:25:00 2012 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Fri Jun 29 07:29:08 2012 Subject: [Histonet] FW: bone regrowth Message-ID: From: Molinari, Betsy Sent: Thursday, June 28, 2012 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: bone regrowth Hi all, I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences. It involves bone regrowth. The sample will vary in size. From my reading, it seems that 5% formic or 10% EDTA is preferred. The researcher asked about acetic acid, but I have never heard as acetic alone as a decal, isn?t it just used to balance pH of the EDTA? It is my understanding that the decal step will be done somewhere else then the sample sent to me. That lab feels that HCL decal will not not affect the sample. I think otherwise. The staining they have talked about is the Goldners Trichrome mainly. The researcher mentioned Safranin O but from my conversations I think they have little knowledge of histology and I work in a cardiovascular lab with my only experience has been with mouse tibia and these samples will be from a larger source. Type 2 collagen was brought up but when I said that was an immuno stain they did not realize that. Si in a nutshell, what decal sol. would be better and what stain? Sorry for the rambling. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. From sarahjones <@t> ufl.edu Fri Jun 29 08:55:09 2012 From: sarahjones <@t> ufl.edu (Jones,Sarah A) Date: Fri Jun 29 08:55:20 2012 Subject: [Histonet] Veterinary histology job at UF Message-ID: Hello Histonetters, The Anatomic Pathology Service within the University of Florida, Veterinary Diagnostic Laboratories (College of Veterinary Medicine) is recruiting for a Laboratory Technician in the Histology Laboratory. The work involves diagnostic veterinary histology and research histology. Full time, 40 hours per week, days, no weekends. The HT (ASCP) is not a requirement for employment. We also do not require the Florida State Laboratory License. A high school diploma and two years of appropriate experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience. Veterinary histology experience a plus. Willing to train the right person. The University of Florida offers a generous benefits package, including one of the best pension plans in the State. Gainesville is a beautiful college town, with large oaks and nearby spring fed rivers for canoeing or kayaking. Housing is very affordable. The job listing can be viewed at: https://jobs.ufl.edu/ Job title: Laboratory Technician job requisition number is 0900939 Sarah A. Jones, HT (ASCP) Histology Lab Manager sarahjones@ufl.edu From twheelock <@t> mclean.harvard.edu Fri Jun 29 09:08:13 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Jun 29 09:08:27 2012 Subject: [Histonet] Hydrometer questions Message-ID: <4FEDB6CD.30509@mclean.harvard.edu> Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 From DKBoyd <@t> chs.net Fri Jun 29 09:17:07 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Fri Jun 29 09:17:22 2012 Subject: [Histonet] Gross macro digital camera In-Reply-To: <17A905B6-1769-40CD-8F1A-467A5C78047C@aol.com> References: <17A905B6-1769-40CD-8F1A-467A5C78047C@aol.com> Message-ID: <7EAFE982E328304DA6CE2B677BB7624639D7F205@TN001WEXMBX12.US.chs.net> We are using a Canon G12 for macros. This is actually our second one. We are very pleased with it. Images are really good. Our Pathology software is PowerPath. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of b427297@aol.com Sent: Thursday, June 28, 2012 11:40 AM To: Marc DeCarlo Cc: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross macro digital camera Would appreciate suggestions for a good digital camera for taking macro photos during necropsy. Any takers? Thanks much. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rjbuesa <@t> yahoo.com Fri Jun 29 09:23:37 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 29 09:23:45 2012 Subject: [Histonet] Hydrometer questions In-Reply-To: <4FEDB6CD.30509@mclean.harvard.edu> References: <4FEDB6CD.30509@mclean.harvard.edu> Message-ID: <1340979817.16889.YahooMailNeo@web121405.mail.ne1.yahoo.com> Tim: When I (and others) wrote you about the "age of the LFB" I at least was referring to the age of the solution. You write now that you are going to try a 1950 LFB and I am sure that you are referring to the powder. As to powdered stains they are quite stable, but it will be nice to find out. ? To your hydrometer question: they are very simple to use, you just place the instrument very delicately into the ethanol sol. contained on a cylinder and it will sink up to a mark on the stem corresponding to the ethanol concentration, BUT there are some precautins to take: ? 1- the density of ethanol (and of any liquid for that matter)?varies with?the temperature; to higher temperature less push of the liquid and if the temp. is high you will get a reading corresponding to a higher ethanol concentration that the real value. 2- the cylinder has to be wide enough, otherwise the stem may come close to the wall of the cylinder and give you also a false reading. ? Your best solution is to prepare fresh ethanol sol. every time you need it and to do that you just calculate how much dist. water you need to add to your ethanol of KNOWN concentration. ? Finally, if you get a hydrometer for ethanol it cannot be used for isopropyl alcohol, because each has a different density. Hydrometers can be bought from Fisher or Thomas Adams (I think they still sell them!). Ren? J. ________________________________ From: Tim Wheelock To: histonet@lists.utsouthwestern.edu Sent: Friday, June 29, 2012 10:08 AM Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri Jun 29 09:46:18 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Jun 29 09:46:59 2012 Subject: [Histonet] Hydrometer temperature correction In-Reply-To: <4FEDB6CD.30509@mclean.harvard.edu> References: <4FEDB6CD.30509@mclean.harvard.edu> Message-ID: There is a chart [ http://www.defalcos.com/tutorials/hy.html ] for temperature correction on ethanol concentration. A lot of information can be found on brewery sites. So . . if you require more help, and since its Friday, visit a local brew house to "ask questions". Tresa :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, June 29, 2012 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estellamireles <@t> gmail.com Fri Jun 29 10:25:27 2012 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Fri Jun 29 10:25:34 2012 Subject: [Histonet] Paperless Histology Message-ID: My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks From LLoss <@t> dermwisconsin.com Fri Jun 29 10:31:40 2012 From: LLoss <@t> dermwisconsin.com (Lee Loss) Date: Fri Jun 29 10:31:49 2012 Subject: [Histonet] Paperless Histology In-Reply-To: References: Message-ID: <2B99CB40EC5CC7409888A2961CBF9688CBAAA6@EX2010.DERM.LOCAL> I have done this in a previous lab by going to a LIS with bar coding throughout the lab. It is not a quick and easy thing to do, but well worth it. I don't know of another way to do it. We are currently going the same route where I'm working now. It can be a long and tedious project - but again, well worth the work to get there. Lee -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From MICHELLE.LAMPHERE <@t> childrens.com Fri Jun 29 10:33:06 2012 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Fri Jun 29 10:32:09 2012 Subject: [Histonet] Histology positionin Dallas Message-ID: We are currently have a histology position open at Children's Medical Center in Dallas. This position requires working knowledge of all aspects of histology including immunohistochemistry. Associates degree in a science discipline preferred, HT or HTL (ASCP) or equivalent or certification eligible. Must acquire the certification within six months of hire date or certification eligibility date. Histology experience is required, must meet the qualification to perform high complexity testing consistent with CLIA requirements . Qualified candidates should apply online at www.childrens.com and go to career links. Sandra Holt, PHR Senior Recruiter Children's Medical Center 214-456-8933 (office) 214-456-1923 (fax) sandra.holt@childrens.com Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

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From norogers <@t> maxhealth.com Fri Jun 29 10:36:12 2012 From: norogers <@t> maxhealth.com (Noah Rogers) Date: Fri Jun 29 10:36:32 2012 Subject: [Histonet] Paperless Histology In-Reply-To: References: Message-ID: Hello Stella, My cousin is an Account Executive for an EMR (Electronic Medical Records) Company, and I can gladly put you in touch with him if that is the sort of paperless option that your Director seeks. Please let me know if I can be of any assistance, Noah Noah Rogers Sr. Recruiter | Maxim Healthcare Services DC/MD/VA - Allied Staffing Division 9525 Georgia Ave | Suite 205 Silver Spring, MD 20910 301.755.2089 (P) 877.748.8793 (F) 703.597.9186 (C) Confidentiality Statement: "The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction." Confidential Health Information Attached. "Health care information is personal and sensitive. It is being faxed/emailed to you after appropriate authorization from the patient/employee or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain this information in a safe, secure and confidential manner. Re-disclosure without additional patient consent or authorization or as permitted by law is prohibited. Unauthorized re-disclosure or failure to maintain the confidentiality of this information could subject you to penalties under Federal and/or State law." Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. From melissa <@t> alliedsearchpartners.com Fri Jun 29 10:42:54 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Fri Jun 29 10:43:05 2012 Subject: [Histonet] Managerial Job Opening in Houston Message-ID: Hi Everyone, I hope everyone out there is having a happy Friday. I wanted to let anyone know, who may be interested, that I have a permanent Histology Manager job in Houston, TX open right now. Please message me for the details and job description if you think you might be interested. Looking forward to hearing from some of you! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From joelleweaver <@t> hotmail.com Fri Jun 29 11:05:42 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jun 29 11:05:47 2012 Subject: [Histonet] Paperless Histology In-Reply-To: References: Message-ID: StellaI have worked at a couple labs that were basically paperless. The papers that they did have were more or less just hard copies for records since some people were not entirely trusting of an entirely electronic system. This was a while ago, and the choices from an IT perspective are surely much better now. Overall, I think it worked pretty well and did save alot of time ( and trees!), and did not have any more "glitches" than any paper based process I have worked in once it got up and running. I am sure any/all vendors of workflow management and LIS applications could provide you with details and options. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 29 Jun 2012 10:25:27 -0500 > From: estellamireles@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Paperless Histology > > My director would like for our histology lab to go totally paperless. > Is there a lab out there that is already paperless? > Are there any software programs for this? > It seems to me that this would include; gross sheets, logging in specimens, > AQ sheets that are delivered with slides, decal log, special stains request. > Would appreciate all the info. Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Fri Jun 29 12:48:07 2012 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Fri Jun 29 12:48:15 2012 Subject: [Histonet] RE: Needing a control block please In-Reply-To: <4b99c1af-bd79-4bd6-ad6e-77de7611465d@ushpwbmsmhp001.one.ads.bms.com> References: <4b99c1af-bd79-4bd6-ad6e-77de7611465d@ushpwbmsmhp001.one.ads.bms.com> Message-ID: Hi everyone - Does anyone have an appendicitis case where they can send me a couple of control blocks? I need a good fibrin control block. If you can help out, please let me know and I'll give you my mailing address. Thanks! Kristie Kristie This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From brannon <@t> alliedsearchpartners.com Fri Jun 29 14:07:31 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Fri Jun 29 14:07:43 2012 Subject: [Histonet] HT/HTL Opening Near Binghamton, NY Message-ID: Allied Search Partners is currently looking for an outstanding Histotech to join a dynamic organization in New York. Location: Binghamton, NY Schedule: Full Time Day Shift To Apply: Please send resume to Brannon@alliedsearchpartners.com or fax to 888 388 7572. Please note that no other information is given at this time about location or organization. Summary and Job Requirements: ? Small Private Laboratory ? HT or HTL ASCP Certification Required ? New York Licensed ? Must meet CLIA requirements to Gross ? Grossing Experience ? Full Healthcare Benefits Please submit resume via email or fax and if you meet requirements one of our recruiters will give you a call. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 From sarahjones <@t> ufl.edu Fri Jun 29 14:52:34 2012 From: sarahjones <@t> ufl.edu (Jones,Sarah A) Date: Fri Jun 29 14:52:41 2012 Subject: [Histonet] more lab humor Message-ID: Pathology Resident Expected to build the building THEN leap over it. Is more powerful than a locomotive, but isn't allowed to play near the tracks. Is expected to get there before the speeding bullet does. Really good at holding breath so they can walk at the bottom of the pond to the other side. Sits quietly while others speak with God. Anonymous Have a great weekend! From Sandra.Harrison3 <@t> va.gov Fri Jun 29 16:17:39 2012 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Jun 29 16:18:13 2012 Subject: [Histonet] Paperless Histology In-Reply-To: References: Message-ID: And what about a paperless procedure manual? We have an electronic system, called Proquis, for our manuals. It sends an e-mail to all the appropriate people, when there is a new procedure to review or when old procedures are due for review. Pretty cool. The only drawback is, most of the time, people would rather grab a notebook to look up a procedure, rather than the cumbersome process of logging in, clicking thru a bunch of menus to view what you could have physically touched and seen in seconds. Plus, you better have a lot of terminals. Or better yet, let's issue I-Pads to all employees. ;-) We live in a brave new world! Sandy H. Histology Supervisor, VA Minneapolis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Jun 29 16:30:32 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jun 29 16:30:38 2012 Subject: [Histonet] RE: bone regrowth In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011393BEA309@SBS2K8.premierlab.local> Betsy I would use formic acid rather than EDTA, especially if you need to run collagen II IHC, we ran into an issue with a study that was decaled in EDTA and the collagen II IHC was not as optimal with the EDTA decal, formic acid is much better for collagen II. We also get good results for safranin O in most samples (except mouse joints) decaled in formic acid. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, June 29, 2012 6:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: bone regrowth From: Molinari, Betsy Sent: Thursday, June 28, 2012 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: bone regrowth Hi all, I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences. It involves bone regrowth. The sample will vary in size. From my reading, it seems that 5% formic or 10% EDTA is preferred. The researcher asked about acetic acid, but I have never heard as acetic alone as a decal, isn't it just used to balance pH of the EDTA? It is my understanding that the decal step will be done somewhere else then the sample sent to me. That lab feels that HCL decal will not not affect the sample. I think otherwise. The staining they have talked about is the Goldners Trichrome mainly. The researcher mentioned Safranin O but from my conversations I think they have little knowledge of histology and I work in a cardiovascular lab with my only experience has been with mouse tibia and these samples will be from a larger source. Type 2 collagen was brought up but when I said that was an immuno stain they did not realize that. Si in a nutshell, what decal sol. would be better and what stain? Sorry for the rambling. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. From asmith <@t> mail.barry.edu Sat Jun 30 14:27:05 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sat Jun 30 14:27:15 2012 Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F092A969BD4@EXCHMB01.isis.local> References: <3903BE18914F4440834F0E620415FFCA0BCC384C@PPWEXD01B.childrens.sea.kids> <493CAA64F203E14E8823737B9EE0E25F092A969BD4@EXCHMB01.isis.local> Message-ID: I have used Vector's ImmPress and ABC secondary antibody systems. I like ImmPress better: it has fewer steps, it gives me less background, and the reagents last longer. Vector's Nova Red has given me better results than any other stain that I have tried. -Allen A. Smith Barry University School of Podiatric Medicine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Wednesday, June 27, 2012 1:38 PM To: 'Lewis, Patrick'; 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, June 27, 2012 9:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates Hi everyone, I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. I love DAKO, but their kits and reagents are expensive. Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. Thanks Patrick. PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. Has anyone tried Spring Bioscience's products? CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly <@t> yahoo.com Sat Jun 30 20:17:09 2012 From: sccrshlly <@t> yahoo.com (Michelle Coker) Date: Sat Jun 30 20:17:13 2012 Subject: [Histonet] Job opening in South Bend, IN (posting for a friend) Message-ID: If interested, email resume to mgiresumes@gmail.com. Must be qualified to perform gross and be able to perform all basic histology duties. Sent from my iPad