[Histonet] counter stain for PAS for fungus

[Jennifer MacDonald]

Add some acetic acid to the fast green. 



On Jul 23, 2012, at 9:11 AM, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:

> Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus.  The fast green stain is simply not staining anymore no matter how long I leave it on the specimen.  Do you have any suggestions on how to correct this or perhaps recommendations for a different counterstain. Our protocol is as follows:
> Periodic Acid 7 min
> quick rinse 
> Schiff solution 20min
> wash for 10min
> Fast green stain 1 min
>  Any help is appreciated.
> Thanks in advance,
> Michele Carr 
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet