From TNMayer <@t> mdanderson.org Tue Jan 3 08:43:57 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Jan 3 08:44:04 2012 Subject: [Histonet] Re: Cutting speed In-Reply-To: <6b8e43c3-3aa0-457e-9ea2-8a627d1b7231@DCPWPRTR01.mdanderson.edu> References: <6b8e43c3-3aa0-457e-9ea2-8a627d1b7231@DCPWPRTR01.mdanderson.edu> Message-ID: Teresa, I agree with Kim. Actually, the speed you desire will come with time, practice and workflow adjustment. You are not far off from where you should be, about 30 blocks/hr. 40 would be great, but you just graduated and got a job. It usually takes me about 2 wks to get adjusted to a new microtome and its quirks. By the end of next week, you should be closer to your goal. If you are really concerned go to the pathologist and the supervisor and have a chat. Go over expectations for all sides and things will get better. Calm down, and ask for help when needed. Take one thing at a time, cutting, workflow, etc. Do your best work, and everything will come as you need it. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Dec 2011 09:18:21 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Cutting speed To: Teresa Moore , "histonet@lists.utsouthwestern.edu" Message-ID: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. ? Best of wishes ? Kim D ________________________________ From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Saturday, December 31, 2011 8:44 AM Subject: [Histonet] Cutting speed I graduated from a histology program in June/11 and just got a job a week ago.? My speed on the microtome is not great.? Everyone says it takes time but I feel my technique may be wrong.? To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself.? My biggest concern is my cutting speed right now.? How long does it take (approx) to do 40 blocks an hour.? Currently, I'm about half that!? I'm panicking and I've only been on the job 8 days.? Help!!!!!!!!!!! -- Teresa Moore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Jan 3 09:39:47 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jan 3 09:40:03 2012 Subject: [Histonet] Cutting speed In-Reply-To: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> References: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> Message-ID: <1F155041-31B6-4C1A-94E4-60319380991D@email.arizona.edu> Teresa, Don't trade quality for speed. I once worked for a pathologist who actually told me that he preferred that we took our time cutting so that the sections were as good as we could make them. He said that it took a lot of the stress of making a diagnosis off of him when he got good slides, especially when the diagnosis was a difficult one. He said to treat the tissue like it came from your Mother or your child. I have worked with people who bragged often and loudly about being fast cutters and their slides looked like it. I agree with the person who advised that you sit down and have a talk with the lab manager to voice your concerns. Everyone should be aware that you are going to do the very best you can while your co-worker is away, even if it takes you a bit longer. Good luck with this! Andi On Dec 31, 2011, at 10:18 AM, Kim Donadio wrote: > My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. > > Best of wishes > > Kim D > > > ________________________________ > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, December 31, 2011 8:44 AM > Subject: [Histonet] Cutting speed > > I graduated from a histology program in June/11 and just got a job a week > ago. My speed on the microtome is not great. Everyone says it takes time > but I feel my technique may be wrong. To make matters worse the only other > histotech in the lab is going on vacation the third week of January and I > will be alone!!!!! I don't have the overall flow of the lab down yet and > have no idea how they expect me to handle the cutting all by myself. My > biggest concern is my cutting speed right now. How long does it take > (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm > panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > -- > Teresa Moore > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histology <@t> gradymem.org Tue Jan 3 09:51:11 2012 From: histology <@t> gradymem.org (Histology Dept) Date: Tue Jan 3 09:51:16 2012 Subject: [Histonet] histology opening Message-ID: We have a full time histotech position open at Grady Memorial Hospital in Chickasha, OK. Must be HT certified or willing to become certified. Hours: 6:00 am to 2:30 pm No weekends. No holidays. Small hospital CAP pathology lab. One pathologists, only 2 histotechs. Go to http://www.gradymem.org/employment.html for information about application. You can apply on line. -- Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/779-2258 histology@gradymem.org From BDeBrosse-Serra <@t> isisph.com Tue Jan 3 09:57:47 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Jan 3 09:58:01 2012 Subject: [Histonet] RE: Cutting speed In-Reply-To: References: <6b8e43c3-3aa0-457e-9ea2-8a627d1b7231@DCPWPRTR01.mdanderson.edu> Message-ID: <493CAA64F203E14E8823737B9EE0E25F091FCE5994@EXCHMB01.isis.local> Teresa, I totally agree with all the advice Toysha and Kim gave you. The cutting speed will come with time and experience. Even though in a clinical environment everybody seems to push speed, the quality of the slide should not suffer. My thoughts are, to take your time to produce good quality slides, do the best you can and the speed will follow. Overall it sounds like you are doing a pretty good job already for a new graduate. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Tuesday, January 03, 2012 6:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Cutting speed Teresa, I agree with Kim. Actually, the speed you desire will come with time, practice and workflow adjustment. You are not far off from where you should be, about 30 blocks/hr. 40 would be great, but you just graduated and got a job. It usually takes me about 2 wks to get adjusted to a new microtome and its quirks. By the end of next week, you should be closer to your goal. If you are really concerned go to the pathologist and the supervisor and have a chat. Go over expectations for all sides and things will get better. Calm down, and ask for help when needed. Take one thing at a time, cutting, workflow, etc. Do your best work, and everything will come as you need it. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Dec 2011 09:18:21 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Cutting speed To: Teresa Moore , "histonet@lists.utsouthwestern.edu" Message-ID: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. ? Best of wishes ? Kim D ________________________________ From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Saturday, December 31, 2011 8:44 AM Subject: [Histonet] Cutting speed I graduated from a histology program in June/11 and just got a job a week ago.? My speed on the microtome is not great.? Everyone says it takes time but I feel my technique may be wrong.? To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself.? My biggest concern is my cutting speed right now.? How long does it take (approx) to do 40 blocks an hour.? Currently, I'm about half that!? I'm panicking and I've only been on the job 8 days.? Help!!!!!!!!!!! -- Teresa Moore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adrienneapergs <@t> hotmail.com Tue Jan 3 10:35:51 2012 From: adrienneapergs <@t> hotmail.com (Adrienne Kavanagh) Date: Tue Jan 3 10:35:54 2012 Subject: [Histonet] Nerve Fiber Density Testing Message-ID: Hi All, I am setting up for a nerve fiber density test. The specifics of the procedure I have been provided with are vague. It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns. Then staining the free-floating sections with a PGP 9.5 antibody. If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish). Thanks! Adrienne From joelleweaver <@t> hotmail.com Tue Jan 3 10:47:39 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jan 3 10:47:49 2012 Subject: [Histonet] Cutting speed In-Reply-To: <1F155041-31B6-4C1A-94E4-60319380991D@email.arizona.edu> References: , <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com>, <1F155041-31B6-4C1A-94E4-60319380991D@email.arizona.edu> Message-ID: Good advice. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: algranth@email.arizona.edu CC: histonet@lists.utsouthwestern.edu Date: Tue, 3 Jan 2012 07:39:47 -0800 Subject: Re: [Histonet] Cutting speed Teresa, Don't trade quality for speed. I once worked for a pathologist who actually told me that he preferred that we took our time cutting so that the sections were as good as we could make them. He said that it took a lot of the stress of making a diagnosis off of him when he got good slides, especially when the diagnosis was a difficult one. He said to treat the tissue like it came from your Mother or your child. I have worked with people who bragged often and loudly about being fast cutters and their slides looked like it. I agree with the person who advised that you sit down and have a talk with the lab manager to voice your concerns. Everyone should be aware that you are going to do the very best you can while your co-worker is away, even if it takes you a bit longer. Good luck with this! Andi On Dec 31, 2011, at 10:18 AM, Kim Donadio wrote: > My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. > > Best of wishes > > Kim D > > > ________________________________ > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, December 31, 2011 8:44 AM > Subject: [Histonet] Cutting speed > > I graduated from a histology program in June/11 and just got a job a week > ago. My speed on the microtome is not great. Everyone says it takes time > but I feel my technique may be wrong. To make matters worse the only other > histotech in the lab is going on vacation the third week of January and I > will be alone!!!!! I don't have the overall flow of the lab down yet and > have no idea how they expect me to handle the cutting all by myself. My > biggest concern is my cutting speed right now. How long does it take > (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm > panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > -- > Teresa Moore > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Tue Jan 3 12:00:44 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Jan 3 12:00:49 2012 Subject: [Histonet] Cutting speed Message-ID: <2572968874904521909@unknownmsgid> Never sacrifice quality for speed. Patient care is priority one, and the lab makes plenty of money. If you are understaffed they need to deal with that, not jeopardize care. You can always contact Healthcare Connections to get vacation coverage, or another agency like that. If you want Healthcare Connections it Comp Health staffing phone numbers feel free to email me. Sent from my Windows Phone From: joelle weaver Sent: 1/3/2012 8:48 AM To: algranth@email.arizona.edu Cc: Histonet Subject: RE: [Histonet] Cutting speed Good advice. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: algranth@email.arizona.edu CC: histonet@lists.utsouthwestern.edu Date: Tue, 3 Jan 2012 07:39:47 -0800 Subject: Re: [Histonet] Cutting speed Teresa, Don't trade quality for speed. I once worked for a pathologist who actually told me that he preferred that we took our time cutting so that the sections were as good as we could make them. He said that it took a lot of the stress of making a diagnosis off of him when he got good slides, especially when the diagnosis was a difficult one. He said to treat the tissue like it came from your Mother or your child. I have worked with people who bragged often and loudly about being fast cutters and their slides looked like it. I agree with the person who advised that you sit down and have a talk with the lab manager to voice your concerns. Everyone should be aware that you are going to do the very best you can while your co-worker is away, even if it takes you a bit longer. Good luck with this! Andi On Dec 31, 2011, at 10:18 AM, Kim Donadio wrote: > My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. > > Best of wishes > > Kim D > > > ________________________________ > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, December 31, 2011 8:44 AM > Subject: [Histonet] Cutting speed > > I graduated from a histology program in June/11 and just got a job a week > ago. My speed on the microtome is not great. Everyone says it takes time > but I feel my technique may be wrong. To make matters worse the only other > histotech in the lab is going on vacation the third week of January and I > will be alone!!!!! I don't have the overall flow of the lab down yet and > have no idea how they expect me to handle the cutting all by myself. My > biggest concern is my cutting speed right now. How long does it take > (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm > panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > -- > Teresa Moore > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Tue Jan 3 12:13:06 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Jan 3 12:13:23 2012 Subject: [Histonet] RE: Nerve Fiber Density Testing (Adrienne Kavanagh) In-Reply-To: References: Message-ID: Adrienne, I have done this procedure before, I think. It sounds real familiar. I was taught it by some folks from Johns Hopkins a few years back for a nerve doctor here in Houston. I will look for the info and send it to you under a separate cover. If I don't have the procedure I may have the contact info for the folks at Johns Hopkins. The doctor in Houston has them come and train his new techs in the procedure. They are real nice and very helpful. Do you screen the biopsies for the fibers as well? I did, and did not like that aspect of the job, I felt that it was the doctor's responsibility to do that, not mine. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ Message: 5 Date: Tue, 3 Jan 2012 11:35:51 -0500 From: Adrienne Kavanagh Subject: [Histonet] Nerve Fiber Density Testing To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I am setting up for a nerve fiber density test. The specifics of the procedure I have been provided with are vague. It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns. Then staining the free-floating sections with a PGP 9.5 antibody. If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish). Thanks! Adrienne ------------------------------ From af46 <@t> buffalo.edu Tue Jan 3 12:26:34 2012 From: af46 <@t> buffalo.edu (Featherstone, Annette) Date: Tue Jan 3 12:26:46 2012 Subject: [Histonet] reply to cutting speed Message-ID: No one should be alone after only a few weeks on the job. What kind of supervised training is that? Sorry, but I would never expect a new recruit to go it alone after such a short time, not in any job. Annette Featherstone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 03, 2012 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 2 You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Cutting speed (Mayer,Toysha N) 2. Re: Cutting speed (Grantham, Andrea L - (algranth)) 3. histology opening (Histology Dept) 4. RE: Cutting speed (Bea DeBrosse-Serra) 5. Nerve Fiber Density Testing (Adrienne Kavanagh) 6. RE: Cutting speed (joelle weaver) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Jan 2012 08:43:57 -0600 From: "Mayer,Toysha N" Subject: [Histonet] Re: Cutting speed To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Teresa, I agree with Kim. Actually, the speed you desire will come with time, practice and workflow adjustment. You are not far off from where you should be, about 30 blocks/hr. 40 would be great, but you just graduated and got a job. It usually takes me about 2 wks to get adjusted to a new microtome and its quirks. By the end of next week, you should be closer to your goal. If you are really concerned go to the pathologist and the supervisor and have a chat. Go over expectations for all sides and things will get better. Calm down, and ask for help when needed. Take one thing at a time, cutting, workflow, etc. Do your best work, and everything will come as you need it. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Dec 2011 09:18:21 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Cutting speed To: Teresa Moore , "histonet@lists.utsouthwestern.edu" Message-ID: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. ? Best of wishes ? Kim D ________________________________ From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Saturday, December 31, 2011 8:44 AM Subject: [Histonet] Cutting speed I graduated from a histology program in June/11 and just got a job a week ago.? My speed on the microtome is not great.? Everyone says it takes time but I feel my technique may be wrong.? To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself.? My biggest concern is my cutting speed right now.? How long does it take (approx) to do 40 blocks an hour.? Currently, I'm about half that!? I'm panicking and I've only been on the job 8 days.? Help!!!!!!!!!!! -- Teresa Moore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 3 Jan 2012 07:39:47 -0800 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] Cutting speed Cc: HISTONET Message-ID: <1F155041-31B6-4C1A-94E4-60319380991D@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" Teresa, Don't trade quality for speed. I once worked for a pathologist who actually told me that he preferred that we took our time cutting so that the sections were as good as we could make them. He said that it took a lot of the stress of making a diagnosis off of him when he got good slides, especially when the diagnosis was a difficult one. He said to treat the tissue like it came from your Mother or your child. I have worked with people who bragged often and loudly about being fast cutters and their slides looked like it. I agree with the person who advised that you sit down and have a talk with the lab manager to voice your concerns. Everyone should be aware that you are going to do the very best you can while your co-worker is away, even if it takes you a bit longer. Good luck with this! Andi On Dec 31, 2011, at 10:18 AM, Kim Donadio wrote: > My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. > > Best of wishes > > Kim D > > > ________________________________ > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, December 31, 2011 8:44 AM > Subject: [Histonet] Cutting speed > > I graduated from a histology program in June/11 and just got a job a > week ago. My speed on the microtome is not great. Everyone says it > takes time but I feel my technique may be wrong. To make matters > worse the only other histotech in the lab is going on vacation the > third week of January and I will be alone!!!!! I don't have the > overall flow of the lab down yet and have no idea how they expect me > to handle the cutting all by myself. My biggest concern is my cutting > speed right now. How long does it take > (approx) to do 40 blocks an hour. Currently, I'm about half that! > I'm panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > -- > Teresa Moore > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Tue, 03 Jan 2012 09:51:11 -0600 From: Histology Dept Subject: [Histonet] histology opening To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; CHARSET=US-ASCII We have a full time histotech position open at Grady Memorial Hospital in Chickasha, OK. Must be HT certified or willing to become certified. Hours: 6:00 am to 2:30 pm No weekends. No holidays. Small hospital CAP pathology lab. One pathologists, only 2 histotechs. Go to http://www.gradymem.org/employment.html for information about application. You can apply on line. -- Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/779-2258 histology@gradymem.org ------------------------------ Message: 4 Date: Tue, 3 Jan 2012 07:57:47 -0800 From: Bea DeBrosse-Serra Subject: [Histonet] RE: Cutting speed To: "'Mayer,Toysha N'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <493CAA64F203E14E8823737B9EE0E25F091FCE5994@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" Teresa, I totally agree with all the advice Toysha and Kim gave you. The cutting speed will come with time and experience. Even though in a clinical environment everybody seems to push speed, the quality of the slide should not suffer. My thoughts are, to take your time to produce good quality slides, do the best you can and the speed will follow. Overall it sounds like you are doing a pretty good job already for a new graduate. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Tuesday, January 03, 2012 6:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Cutting speed Teresa, I agree with Kim. Actually, the speed you desire will come with time, practice and workflow adjustment. You are not far off from where you should be, about 30 blocks/hr. 40 would be great, but you just graduated and got a job. It usually takes me about 2 wks to get adjusted to a new microtome and its quirks. By the end of next week, you should be closer to your goal. If you are really concerned go to the pathologist and the supervisor and have a chat. Go over expectations for all sides and things will get better. Calm down, and ask for help when needed. Take one thing at a time, cutting, workflow, etc. Do your best work, and everything will come as you need it. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Sat, 31 Dec 2011 09:18:21 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Cutting speed To: Teresa Moore , "histonet@lists.utsouthwestern.edu" Message-ID: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. ? Best of wishes ? Kim D ________________________________ From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Saturday, December 31, 2011 8:44 AM Subject: [Histonet] Cutting speed I graduated from a histology program in June/11 and just got a job a week ago.? My speed on the microtome is not great.? Everyone says it takes time but I feel my technique may be wrong.? To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself.? My biggest concern is my cutting speed right now.? How long does it take (approx) to do 40 blocks an hour.? Currently, I'm about half that!? I'm panicking and I've only been on the job 8 days.? Help!!!!!!!!!!! -- Teresa Moore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 3 Jan 2012 11:35:51 -0500 From: Adrienne Kavanagh Subject: [Histonet] Nerve Fiber Density Testing To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I am setting up for a nerve fiber density test. The specifics of the procedure I have been provided with are vague. It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns. Then staining the free-floating sections with a PGP 9.5 antibody. If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish). Thanks! Adrienne ------------------------------ Message: 6 Date: Tue, 3 Jan 2012 16:47:39 +0000 From: joelle weaver Subject: RE: [Histonet] Cutting speed To: Cc: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good advice. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: algranth@email.arizona.edu CC: histonet@lists.utsouthwestern.edu Date: Tue, 3 Jan 2012 07:39:47 -0800 Subject: Re: [Histonet] Cutting speed Teresa, Don't trade quality for speed. I once worked for a pathologist who actually told me that he preferred that we took our time cutting so that the sections were as good as we could make them. He said that it took a lot of the stress of making a diagnosis off of him when he got good slides, especially when the diagnosis was a difficult one. He said to treat the tissue like it came from your Mother or your child. I have worked with people who bragged often and loudly about being fast cutters and their slides looked like it. I agree with the person who advised that you sit down and have a talk with the lab manager to voice your concerns. Everyone should be aware that you are going to do the very best you can while your co-worker is away, even if it takes you a bit longer. Good luck with this! Andi On Dec 31, 2011, at 10:18 AM, Kim Donadio wrote: > My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. > > Best of wishes > > Kim D > > > ________________________________ > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, December 31, 2011 8:44 AM > Subject: [Histonet] Cutting speed > > I graduated from a histology program in June/11 and just got a job a week > ago. My speed on the microtome is not great. Everyone says it takes time > but I feel my technique may be wrong. To make matters worse the only other > histotech in the lab is going on vacation the third week of January and I > will be alone!!!!! I don't have the overall flow of the lab down yet and > have no idea how they expect me to handle the cutting all by myself. My > biggest concern is my cutting speed right now. How long does it take > (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm > panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > -- > Teresa Moore > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 2 *************************************** From Heidi.Hawthorne <@t> onassignment.com Tue Jan 3 12:46:41 2012 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Tue Jan 3 12:46:48 2012 Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B8326DBEE3F09@oasslcexm01.oaifield.onasgn.com> Hello! We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco. Day/early morning shift available working in a hospital lab. Requirements: At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at: http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 From Robyn.Rosenberg <@t> amnhealthcare.com Tue Jan 3 12:57:28 2012 From: Robyn.Rosenberg <@t> amnhealthcare.com (Robyn Rosenberg) Date: Tue Jan 3 12:57:49 2012 Subject: [Histonet] Certified Histologist-Relocation to TX paid! Message-ID: <2030E2FECB4DAB48B4C31D919380F3FF112DAFBC@amnmail.ahs.int> **Award Winning Facility in West Texas is Hiring Due to Largest Expansion in History!!!** Winner of the Gallup "Great Place to Work" Award for 5 years in a row!!! 185 Miles West of Dallas; Very Low Cost of Living Relocation assistance - up to $3000 ($1000-1500 within TX) Certified Histotechnologist or Certified Histotechnician Position Summary: Cuts, stains, mounts, and studies specimens of human tissue to provide data on functioning of tissues and organs, causes or progress of disease, following established standards and practices. Experience: HTL (ASCP) Certification Required (Histotechnologist) Bachelor's degree required (Histotechnologist) Experience - 5 years minimum Should be able to embed tissue, cut blocks, staining, process cytology specimens, aid in grossing. Click here to see a benefits summary: www.ehendrick.org/employment/benefits.htm Hours: 8 hour shifts, variable - department is open from 4 am to 5pm Requirements: Histo Cert (ASCP), B.S. in related field, 5+ yrs current histo experience Robyn Rosenberg Recruiter, Recruitment Process Outsourcing AMN Healthcare Direct Phone: (858) 314-7460 Direct Fax: (866) 652-6931 12400 High Bluff Drive, San Diego CA 92130 robyn.rosenberg@amnhealthcare.com www.amnhealthcare.com NYSE: AHS From joelleweaver <@t> hotmail.com Tue Jan 3 13:04:58 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jan 3 13:05:03 2012 Subject: [Histonet] Cutting speed In-Reply-To: <2572968874904521909@unknownmsgid> References: <2572968874904521909@unknownmsgid> Message-ID: I think that you want to contact Teresa Moore, I am good, been through this whole process/experience myself- but I have more time out, and "old shoe now"- she has a great attitude, and was a super student. I don't have her email saved on here, but I hope that she sees your messages and I am glad to see all the support she is getting here!Joelle Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: pathlocums@gmail.com > Date: Tue, 3 Jan 2012 10:00:44 -0800 > Subject: RE: [Histonet] Cutting speed > To: joelleweaver@hotmail.com; algranth@email.arizona.edu > CC: histonet@lists.utsouthwestern.edu > > Never sacrifice quality for speed. Patient care is priority one, and > the lab makes plenty of money. If you are understaffed they need to > deal with that, not jeopardize care. You can always contact Healthcare > Connections to get vacation coverage, or another agency like that. If > you want Healthcare Connections it Comp Health staffing phone numbers > feel free to email me. > > Sent from my Windows Phone > From: joelle weaver > Sent: 1/3/2012 8:48 AM > To: algranth@email.arizona.edu > Cc: Histonet > Subject: RE: [Histonet] Cutting speed > > Good advice. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > From: algranth@email.arizona.edu > CC: histonet@lists.utsouthwestern.edu > Date: Tue, 3 Jan 2012 07:39:47 -0800 > Subject: Re: [Histonet] Cutting speed > > Teresa, > Don't trade quality for speed. I once worked for a pathologist who > actually told me that he preferred that we took our time cutting so > that the sections were as good as we could make them. He said that it > took a lot of the stress of making a diagnosis off of him when he got > good slides, especially when the diagnosis was a difficult one. He > said to treat the tissue like it came from your Mother or your child. > I have worked with people who bragged often and loudly about being > fast cutters and their slides looked like it. > I agree with the person who advised that you sit down and have a talk > with the lab manager to voice your concerns. Everyone should be aware > that you are going to do the very best you can while your co-worker is > away, even if it takes you a bit longer. > Good luck with this! > > Andi > > > > > > On Dec 31, 2011, at 10:18 AM, Kim Donadio wrote: > > > My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. > > > > Best of wishes > > > > Kim D > > > > > > ________________________________ > > From: Teresa Moore > > To: histonet@lists.utsouthwestern.edu > > Sent: Saturday, December 31, 2011 8:44 AM > > Subject: [Histonet] Cutting speed > > > > I graduated from a histology program in June/11 and just got a job a week > > ago. My speed on the microtome is not great. Everyone says it takes time > > but I feel my technique may be wrong. To make matters worse the only other > > histotech in the lab is going on vacation the third week of January and I > > will be alone!!!!! I don't have the overall flow of the lab down yet and > > have no idea how they expect me to handle the cutting all by myself. My > > biggest concern is my cutting speed right now. How long does it take > > (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm > > panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > > > -- > > Teresa Moore > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Tue Jan 3 13:11:19 2012 From: nelsonrnch <@t> verizon.net (PATTI NELSON HT(ASCP)) Date: Tue Jan 3 13:11:22 2012 Subject: [Histonet] HT/HTL Position in Southern California and Central California Message-ID: <1325617879.71969.YahooMailNeo@web84502.mail.ne1.yahoo.com> Good morning Histo-Land, I am assisting?two new GI labs locate HT/HTLS. One is in located in Southern?California and?one in Central California.? New GI lab in beautiful Santa Monica California is seeking a Certified HT/HTL for a full time position. Applicant must be Self Motivated, Detailed Oriented and wants to advance in their career. Position requires knowledge of QA, QC, Grossing and all functions of a Histology lab. Immediate position available. Position offers attractive and competitive?package. Call or e-mail me for more detailed information. ALSO; New GI lab in beautiful Santa Maria California is seeking a Certified HT/HTL for a full time position. Applicant must be Self Motivated, Detailed Oriented and wants to advance in their career. Position requires knowledge of QA, QC, Grossing and all?functions of a Histology lab. Position offers attractive and competitive?package. Call or e-mail me for more detailed information. BEST REGARDS, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From amosbrooks <@t> gmail.com Tue Jan 3 14:30:18 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jan 3 14:30:23 2012 Subject: [Histonet] Nerve Fiber Density Testing Message-ID: Hi, I did this same thing some years back. Keeping the free floating skin bx sections from breaking up during the IHC was tricky. Sometimes it is easiest to transfer the solutions in & out of the plate wells rather than trying to lift the sections with a loop. It can end up with good results though. Ping me if you have any questions. (Hopefully I'll remember) Best of luck, Amos On Tue, Jan 3, 2012 at 1:00 PM, wrote: > Message: 5 > Date: Tue, 3 Jan 2012 11:35:51 -0500 > From: Adrienne Kavanagh > Subject: [Histonet] Nerve Fiber Density Testing > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hi All, > > I am setting up for a nerve fiber density test. The specifics of the > procedure I have been provided with are vague. > > It basically involves taking a punch biopsy of skin, fixing in PLP, > cryoprotecting, embedding in a sucrose solution to a frozen stage, cover > with dry ice, and section using a sliding microtome at 50 microns. Then > staining the free-floating sections with a PGP 9.5 antibody. > > > If anyone is performing this testing, I would appreciate your response > (you may contact me privately if you wish). > > Thanks! > > Adrienne > From hsingh <@t> qcsciences.com Tue Jan 3 14:32:30 2012 From: hsingh <@t> qcsciences.com (Harpreet Singh) Date: Tue Jan 3 14:32:34 2012 Subject: [Histonet] Epidermal Nerve Fiber Density testing Message-ID: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> I too am interested in setting up this test at my lab. I was curious as to why PLP was being used as a collection medium and if sections less than 50 um could be used. Other than fresh biopsies what other material would be suitable for validations? Harpreet Singh, M.S. HTL (ASCP) In response to Message: 5 Date: Tue, 3 Jan 2012 11:35:51 -0500 From: Adrienne Kavanagh Subject: [Histonet] Nerve Fiber Density Testing To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I am setting up for a nerve fiber density test. The specifics of the procedure I have been provided with are vague. It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns. Then staining the free-floating sections with a PGP 9.5 antibody. If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish). Thanks! Adrienne From amber.mckenzie <@t> gastrodocs.net Tue Jan 3 15:06:00 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jan 3 15:04:36 2012 Subject: [Histonet] new anitbody In-Reply-To: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> Message-ID: <5A33C952BB67F4468AF1F36D739212BC13AABF@JERRY.Gia.com> I know when you get a new instrument you have to validate every antibody against previous stained slides on the original instrument, but what actions must be taken for a new antibody? Besides, putting the protocol in and running control slides. From amber.mckenzie <@t> gastrodocs.net Tue Jan 3 15:09:48 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jan 3 15:08:26 2012 Subject: [Histonet] time off References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> Message-ID: <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? From Bauer.Karen <@t> mayo.edu Tue Jan 3 15:19:54 2012 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jan 3 15:19:59 2012 Subject: [Histonet] time off In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> Message-ID: <53FC421CC200C5429929EDE6C3676F3001BBE5D2@msgebe34> Hi Amber, I have a Holiday Rotation List that I use for my staff. I started the list off with "seniority", but then everytime a tech uses PTO around a holiday (either before or after), they go to the bottom of the list for that holiday. This ensures that all techs have a chance to take off and it's equal for everyone. There are some techs that ask for holidays every year, but I post the holiday rotations and they know they might not get it. Even though they asked 6 months in advance, they have to wait to see if the techs at the top of the list will be utilizing PTO for that particular holiday. If not, they can have it off... And then go to the bottom of the list again. I update the rotation list every year and post it... It's helped a lot with holiday fairness. I can send you the form if you would like... Just so you can see what I'm talking about. Hope this helps, Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor - Pathology Department MOHS Lab Supervisor - Dermatology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 03, 2012 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Tue Jan 3 15:31:46 2012 From: mucram11 <@t> comcast.net (mucram11@comcast.net) Date: Tue Jan 3 15:31:50 2012 Subject: [Histonet] HT or HTL Position in Little Rock AR Message-ID: <0055FB90BD3143B49B2855438802AEEA@PamPC> We have a position at the University of Arkansas for Medical Sciences for a registered HT (ASCP). This is a fulltime position with either early or late shift possibilities. It is an immediate opportunity. We will require current registration with ASCP. Recruiters need not answer as we are not allowed to use the services and it will be a waste of time for us to even discuss it. I am sorry this is a state University and it has rules we can change. Pam Marcum AP Histology Supervisor UAMS 501-686-7554 From trathborne <@t> somerset-healthcare.com Tue Jan 3 15:32:24 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 3 15:32:42 2012 Subject: [Histonet] RE: time off In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7BAC1@SMCMAIL01.somerset-healthcare.com> We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during "peak vacation time". For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 03, 2012 4:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Rcartun <@t> harthosp.org Tue Jan 3 15:41:32 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 3 15:41:38 2012 Subject: [Histonet] new anitbody In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13AABF@JERRY.Gia.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AABF@JERRY.Gia.com> Message-ID: <4F032FBC.7400.0077.1@harthosp.org> You need to prove that the antibody labels its intended target and that there is no cross-reactivity with unrelated proteins. The number of cases needed to do this will depend on the antibody's "track record" and its purpose. For example, antibodies used to identify predictive targets (ER, PR, and HER2 in breast CA) will require more cases to complete your validation since patients are being treated based on the presence or absence of these targets. Some IHC experts will tell you that you need to run 25, 50, or maybe even 100 cases (positive and negative) to validate an antibody. I tell people that you need to run enough cases so that your pathologists feel comfortable interpretating that test. After all, they are the ones that sign-off on these tests. When bringing a new antibody on-board, read the antibody product data sheet and the pathology literature, and then sit down with your pathologist and create a "reasonable" validation plan. In my opinion, far too much money is being wasted running unnecessary slides for validation purposes. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Amber McKenzie 1/3/2012 4:06 PM >>> I know when you get a new instrument you have to validate every antibody against previous stained slides on the original instrument, but what actions must be taken for a new antibody? Besides, putting the protocol in and running control slides. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Tue Jan 3 16:40:36 2012 From: abeharry798 <@t> gmail.com (Andrea) Date: Tue Jan 3 16:31:25 2012 Subject: [Histonet] RE: time off In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7BAC1@SMCMAIL01.somerset-healthcare.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711F7BAC1@SMCMAIL01.somerset-healthcare.com> Message-ID: We also have a policy on the number of people that can be off during peak times. Our vacation schedule runs from June to June of the following year. Staff have up until a deadline of January 31 to put in their request for the next vacation year, whether it is one day or weeks. The requests handed in by this time period are granted based on seniority. ( in our institution they figure it's one of the only perks to being a senior!) After the January 31 deadline it is first come first serve. All vacation requests must be submitted on a vacation request form and time stamped when handed in. This way if two people ask for the same day the person who handed it in with an earlier time stamp is granted the time off. All of this is written in our scheduling guidelines. It seems to work pretty well. On 2012-01-03, at 4:32 PM, "Rathborne, Toni" wrote: > We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during "peak vacation time". For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, January 03, 2012 4:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] time off > > > Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Wed Jan 4 02:10:55 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Jan 4 02:11:02 2012 Subject: [Histonet] RE: time off In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DDFA58A1A@FWDCWPMSGCMS09.hca.corpad.net> We usually go by "first asked" days off. (We keep a calendar for this purpose) But for holidays we rotate. If someone had the day after Thanksgiving off last year they let the next in line have the choice.( I keep the previous years calendars for checking) If no one else wants it they can then have it. It has worked out pretty well over the years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 03, 2012 4:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Jan 4 07:46:56 2012 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Jan 4 07:47:15 2012 Subject: [Histonet] RE: time off In-Reply-To: References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711F7BAC1@SMCMAIL01.somerset-healthcare.com> Message-ID: <4F041200.2B7F.00C9.1@geisinger.edu> I've witnessed that granting time by seniority lets you open to abuse. I know of an employee with high seniority who takes the whole week after Christmas every year. Our policy allows only one person off per day per shift. So then no one else ever can take off at Christmastime. Her peers complain to management about it, but they won't say anything to her. Just my two cents. >>> Andrea 1/3/2012 5:40 PM >>> We also have a policy on the number of people that can be off during peak times. Our vacation schedule runs from June to June of the following year. Staff have up until a deadline of January 31 to put in their request for the next vacation year, whether it is one day or weeks. The requests handed in by this time period are granted based on seniority. ( in our institution they figure it's one of the only perks to being a senior!) After the January 31 deadline it is first come first serve. All vacation requests must be submitted on a vacation request form and time stamped when handed in. This way if two people ask for the same day the person who handed it in with an earlier time stamp is granted the time off. All of this is written in our scheduling guidelines. It seems to work pretty well. On 2012-01-03, at 4:32 PM, "Rathborne, Toni" wrote: > We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during "peak vacation time". For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, January 03, 2012 4:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] time off > > > Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From NLEMKE1 <@t> hfhs.org Wed Jan 4 08:07:04 2012 From: NLEMKE1 <@t> hfhs.org (Lemke, Nancy) Date: Wed Jan 4 08:07:12 2012 Subject: [Histonet] Great job opportunity Message-ID: <66EF8D68E063C044B9AC46E99E3502BF017DBC3483@RHWMBX01.corp.ds.hfhs.org> Good morning Histoland! I would like to tell you about a wonderful job in a great lab with very special people. I am retiring but my job isn't! I run the histo core for the Hermelin Brain Tumor Center at Henry Ford Hospital in Detroit, MI. I have a group of Neurosurgery Research investigators for whom I manage all aspects of the histo core, including supervising one histotechnician. I also run all immunos for of my investigators, and this includes many using novel RUO antibodies on human, and xenograft tissues. This position requires that a candidate be a self starter, able to work independently and also able to communicate well either verbally or in writing. Henry Ford is an excellent place to work and offers wonderful benefits to its employees. The position has been titled senior medical technologist and is number 66733. The job description for this title does not mention histology but rest assured that it is a histotechnology position. Please use the link http://www.henryford.com/ and search for this job number if you are interested. I would be happy to respond to any interested job seekers if you have questions. This is a unique opportunity in a great setting. Nancy W Lemke Research Coordinator Hermelin Brain Tumor Center rm 3115 E&R Bldg Neurosurgery Research Henry Ford Hospital 2799 W Grand Blvd Detroit, MI 48202 ________________________________ CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. From amber.mckenzie <@t> gastrodocs.net Wed Jan 4 09:13:34 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Jan 4 09:12:16 2012 Subject: [Histonet] RE: time off In-Reply-To: References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711F7BAC1@SMCMAIL01.somerset-healthcare.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC13ACDF@JERRY.Gia.com> How do you handle PRN's? If they turn in time off do you grant them before the FT staff or don't worry about their days since they are fill in people? Do PRN's count as having 1 person off at a time along with the FT staff if you only allow 1 person off per day? -----Original Message----- From: Andrea [mailto:abeharry798@gmail.com] Sent: Tuesday, January 03, 2012 4:41 PM To: Rathborne, Toni Cc: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: time off We also have a policy on the number of people that can be off during peak times. Our vacation schedule runs from June to June of the following year. Staff have up until a deadline of January 31 to put in their request for the next vacation year, whether it is one day or weeks. The requests handed in by this time period are granted based on seniority. ( in our institution they figure it's one of the only perks to being a senior!) After the January 31 deadline it is first come first serve. All vacation requests must be submitted on a vacation request form and time stamped when handed in. This way if two people ask for the same day the person who handed it in with an earlier time stamp is granted the time off. All of this is written in our scheduling guidelines. It seems to work pretty well. On 2012-01-03, at 4:32 PM, "Rathborne, Toni" wrote: > We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during "peak vacation time". For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, January 03, 2012 4:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] time off > > > Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jan 4 09:41:34 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 4 09:41:47 2012 Subject: AW: [Histonet] time off In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> Message-ID: We have a list, that can be filled with holiday-wishes. We arrange a special date (for summer holidays e.g. in february) until then the wishes had to be declared. If there are no troubles concerning the number of workers simultanously off, the holidays are fixed. If there are troubles, we speak about that in a meeting. We have no yearly rotation, but something like a generation-agreement. Mummies with children in school or kindergarden have the first choice during school-holidays. Co-workers without children or with older children are usually happy, not to take vacancies at the same time. So over the years, we were happy to reach an everybody-happy-state. Hope, it will go on. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amber McKenzie Gesendet: Dienstag, 03. J?nner 2012 22:10 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmburns9 <@t> gmail.com Wed Jan 4 11:10:26 2012 From: dmburns9 <@t> gmail.com (Douglas M Burns) Date: Wed Jan 4 11:10:33 2012 Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Message-ID: Hello, Histonetters, To continue my (sad) string posts about CryoJane problems, we have now switched to the 4X slides, and we still observe the tape pulling pieces of the section off the slide. Sometimes the pieces are very small, sometimes they are large, and at times a whole region of the section comes off with the tape. We have now tried many different things to correct this: 1) pulling the tape off the section in many different ways, 2) pulling tape off at many different angles & speeds, 3) several different temperatures, 4) with many different mental attitudes, 5) with sections of different thickness, 6) light rolling of tape and section versus ferocious rolling, etc., etc. So far, no dice; we are puzzled. Does anyone have more ideas about what to adjust. We think that this really should work, but at the same time, we can't seem to locate many users of the CryoJane system. So, CryoJane enthusiasts, this is the time to talk about why you like it, or how you do it. thanks again --------------- Doug Burns, MBRF, Kansas City From brian1975 <@t> email.com Wed Jan 4 11:33:49 2012 From: brian1975 <@t> email.com (Brian foster) Date: Wed Jan 4 11:33:57 2012 Subject: [Histonet] Paraffin Blocks Cracking Message-ID: <20120104173350.109560@gmx.com> Hello all, I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? From Mandy.Bell <@t> chomp.org Wed Jan 4 11:39:50 2012 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Wed Jan 4 11:39:59 2012 Subject: [Histonet] Biocare TB antibody Message-ID: <87583127C45A5B4994B162C674378265037DAD@EXMAIL1P.chomp.org> Hi, I was wondering if anyone has used the Biocare TB antibody on the Ventana XT would be willing to share their protocol. We are having trouble getting this antibody to work for us without background staining. Thanks, Mandy Bell HTL(ASCP) Community Hospital of the Monterey Peninsula 2 Harris Court Suite B3/B4 Monterey, CA 93940 (831) 647-4791 From rjbuesa <@t> yahoo.com Wed Jan 4 11:50:13 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 4 11:50:16 2012 Subject: [Histonet] Paraffin Blocks Cracking In-Reply-To: <20120104173350.109560@gmx.com> Message-ID: <1325699413.16070.YahooMailClassic@web65716.mail.ac4.yahoo.com> Rapid cooling is the problem, but I do not think that -5?C can cause it. The effect is more noticeable in large but thin blocks. Ren? J. --- On Wed, 1/4/12, Brian foster wrote: From: Brian foster Subject: [Histonet] Paraffin Blocks Cracking To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 4, 2012, 12:33 PM Hello all, I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Jan 4 11:55:56 2012 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Jan 4 11:55:57 2012 Subject: [Histonet] Paraffin Blocks Cracking In-Reply-To: <1325699413.16070.YahooMailClassic@web65716.mail.ac4.yahoo.com> Message-ID: <1488794099.223852.1325699756078.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I agree however; leaving blocks on the cold plate too long will also cause cracking of blocks even at -2C. ----- Original Message ----- From: "Rene J Buesa" To: histonet@lists.utsouthwestern.edu, "Brian foster" Sent: Wednesday, January 4, 2012 11:50:13 AM Subject: Re: [Histonet] Paraffin Blocks Cracking Rapid cooling is the problem, but I do not think that -5?C can cause it. The effect is more noticeable in large but thin blocks. Ren? J. --- On Wed, 1/4/12, Brian foster wrote: From: Brian foster Subject: [Histonet] Paraffin Blocks Cracking To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 4, 2012, 12:33 PM Hello all, I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Wed Jan 4 12:28:49 2012 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Wed Jan 4 12:28:55 2012 Subject: [Histonet] Epic LIS Message-ID: <938D716CD445614ABBB817517557B6F404BF2A96DA@NADCWPMSGCMS09.hca.corpad.net> Is anyone currently using the EPIC-beaker LIS system? Can you contact me if you are? Thanks Jessica Vacca From nto <@t> stowers.org Wed Jan 4 12:31:40 2012 From: nto <@t> stowers.org (Thomas, Nancy) Date: Wed Jan 4 12:31:50 2012 Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF994ECC286@EXCHMB-02.stowers-institute.org> Hi Douglas, We have a cryojane system, but do not use it too often. So this might not be expert advise, but I do remember that we would sometimes flash the slide more than once. I was just checking in the procedure manual that came with the equipment and it does not mention to do this. Still, we did it and it helped. Also, looking back at some old histonet advise, I saw that someone posted that the 1x slides worked better than the 4x. We also found this to be true. I spoke with a salesperson at the NSH a few years ago and he said to use the 4x. So we did try that but found it not to be the cure. Also, there is mention of making sure your knife blade is good and sharp. Also play around with different knife angles. If you are using a disposable knife and cannot get good results after all of your changes, you may need to try a tungsten-carbide knife. Hope this helps! Nancy Thomas Stowers Institute Kansas City, MO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns Sent: Wednesday, January 04, 2012 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Nancy Thomas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns Sent: Wednesday, January 04, 2012 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Hello, Histonetters, To continue my (sad) string posts about CryoJane problems, we have now switched to the 4X slides, and we still observe the tape pulling pieces of the section off the slide. Sometimes the pieces are very small, sometimes they are large, and at times a whole region of the section comes off with the tape. We have now tried many different things to correct this: 1) pulling the tape off the section in many different ways, 2) pulling tape off at many different angles & speeds, 3) several different temperatures, 4) with many different mental attitudes, 5) with sections of different thickness, 6) light rolling of tape and section versus ferocious rolling, etc., etc. So far, no dice; we are puzzled. Does anyone have more ideas about what to adjust. We think that this really should work, but at the same time, we can't seem to locate many users of the CryoJane system. So, CryoJane enthusiasts, this is the time to talk about why you like it, or how you do it. thanks again --------------- Doug Burns, MBRF, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Wed Jan 4 12:31:48 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 4 12:31:52 2012 Subject: [Histonet] RE: time off In-Reply-To: References: Message-ID: When I did it, seniority first, rotating holidays, but birthdays around holidays were a sore spot. I usually gave the birthday and one day, but not a whole week if it fell within a holiday week. This allowed those who wanted to rotate holidays to do so, but they could only take that one. No more for the season unless nobody wanted it. As for the length of time, the whole year was fine, but those with kids had to understand that spring break had to be rotated as well. PRN's are icing, so I tried to accommodate unless they were permanent. One tech off per day is good, depending on workload, but if possible half days could be suggested for all. Those that come in before 7 work the first half day, and those that come in later work the second half of the day overlapping by at least 1 hr. Letting parents off for school holidays is ok sometime, but what about the others who may want to be off then? Also, if your lab has multiple parents of children, what is the pecking order? What about those who have multiple kids, say a 2, 4, 6, and 12 y/old? They might be off for holidays for many consecutive years, I don't think so. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org -----Original Message----- ------------------------------ Message: 2 Date: Wed, 4 Jan 2012 15:13:34 +0000 From: Amber McKenzie Subject: RE: [Histonet] RE: time off Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC13ACDF@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" How do you handle PRN's? If they turn in time off do you grant them before the FT staff or don't worry about their days since they are fill in people? Do PRN's count as having 1 person off at a time along with the FT staff if you only allow 1 person off per day? -----Original Message----- From: Andrea [mailto:abeharry798@gmail.com] Sent: Tuesday, January 03, 2012 4:41 PM To: Rathborne, Toni Cc: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: time off We also have a policy on the number of people that can be off during peak times. Our vacation schedule runs from June to June of the following year. Staff have up until a deadline of January 31 to put in their request for the next vacation year, whether it is one day or weeks. The requests handed in by this time period are granted based on seniority. ( in our institution they figure it's one of the only perks to being a senior!) After the January 31 deadline it is first come first serve. All vacation requests must be submitted on a vacation request form and time stamped when handed in. This way if two people ask for the same day the person who handed it in with an earlier time stamp is granted the time off. All of this is written in our scheduling guidelines. It seems to work pretty well. On 2012-01-03, at 4:32 PM, "Rathborne, Toni" wrote: > We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during "peak vacation time". For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, January 03, 2012 4:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] time off > > > Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? > ------------------------------ Message: 3 Date: Wed, 4 Jan 2012 16:41:34 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] time off To: "'Amber McKenzie'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have a list, that can be filled with holiday-wishes. We arrange a special date (for summer holidays e.g. in february) until then the wishes had to be declared. If there are no troubles concerning the number of workers simultanously off, the holidays are fixed. If there are troubles, we speak about that in a meeting. We have no yearly rotation, but something like a generation-agreement. Mummies with children in school or kindergarden have the first choice during school-holidays. Co-workers without children or with older children are usually happy, not to take vacancies at the same time. So over the years, we were happy to reach an everybody-happy-state. Hope, it will go on. Gudrun From vavalos <@t> allergydermatology.com Wed Jan 4 12:33:33 2012 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Jan 4 12:33:43 2012 Subject: [Histonet] Paraffin Blocks Cracking In-Reply-To: <20120104173350.109560@gmx.com> References: <20120104173350.109560@gmx.com> Message-ID: I have also noticed that the temperature in the lab makes a big difference. Now that it is colder outside and some heaters kick on check your temps. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian foster Sent: Wednesday, January 04, 2012 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Blocks Cracking Hello all, I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Wed Jan 4 12:43:23 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 4 12:43:26 2012 Subject: [Histonet] RE: Paraffin blocks cracking In-Reply-To: References: Message-ID: Also, using 100% alcohol on the cold plate induces cracking. Freeze spray does too, although some people will never admit to using it. Both of these things induce the rapid cooling effect. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ ------------------------------ Message: 8 Date: Wed, 4 Jan 2012 17:55:56 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] Paraffin Blocks Cracking To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Message-ID: <1488794099.223852.1325699756078.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I agree however; leaving blocks on the cold plate too long will also cause cracking of blocks even at -2C. ----- Original Message ----- From: "Rene J Buesa" To: histonet@lists.utsouthwestern.edu, "Brian foster" Sent: Wednesday, January 4, 2012 11:50:13 AM Subject: Re: [Histonet] Paraffin Blocks Cracking Rapid cooling is the problem, but I do not think that -5??C can cause it. The effect is more noticeable in large but thin blocks. Ren?? J. --- On Wed, 1/4/12, Brian foster wrote: From: Brian foster Subject: [Histonet] Paraffin Blocks Cracking To: histonet@lists.utsouthwestern.edu Date: Wednesday, January 4, 2012, 12:33 PM Hello all, I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 4 *************************************** From mward <@t> wakehealth.edu Wed Jan 4 13:04:35 2012 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Wed Jan 4 13:04:42 2012 Subject: [Histonet] RE: Epic LIS In-Reply-To: <938D716CD445614ABBB817517557B6F404BF2A96DA@NADCWPMSGCMS09.hca.corpad.net> References: <938D716CD445614ABBB817517557B6F404BF2A96DA@NADCWPMSGCMS09.hca.corpad.net> Message-ID: I would also be interested in any replies concerning EPIC-beaker. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Wednesday, January 04, 2012 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Epic LIS Is anyone currently using the EPIC-beaker LIS system? Can you contact me if you are? Thanks Jessica Vacca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jan 4 13:11:31 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 4 13:11:35 2012 Subject: AW: [Histonet] Paraffin Blocks Cracking In-Reply-To: <20120104173350.109560@gmx.com> References: <20120104173350.109560@gmx.com> Message-ID: <7A6D59BAA7C94CBC95ACDF68C13DCD6F@dielangs.at> I've read somewhere (?), that too slow hardening is the culprit for sprinkled blocks. I can also imagine, that the type of paraffine and special additives can make a difference. Our coolplate is set at -10?C and had never problems with cracking paraffin. I think it's similar to freezing. The faster the freezing the smaller the ice-cristals. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Brian foster Gesendet: Mittwoch, 04. J?nner 2012 18:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Paraffin Blocks Cracking Hello all, I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jan 4 17:13:00 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 4 17:13:27 2012 Subject: [Histonet] time off In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> Message-ID: <7ADE179A9DD94254A6334D6665E46B37@JoePC> I start with the holidays. We rotate the major ones every year. When I set up the private lab I used to work at, I had it put in the H.R. policy that an employee could take 2 of 3 end of year holidays (Thanksgiving, Christmas, New Year's) on a rotating basis. If no one else put in for those times, then I would grant that request. I went out only 6 months at a time. We were expanding exponentially and I couldn't project what the work load would be like next week, never mind 6 months from now. All the other holidays were not a problem. JTT ----- Original Message ----- From: "Amber McKenzie" To: Sent: Tuesday, January 03, 2012 3:09 PM Subject: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kasaim <@t> mail.nih.gov Wed Jan 4 22:13:32 2012 From: kasaim <@t> mail.nih.gov (Kasai, Miki (NIH/NCI) [E]) Date: Wed Jan 4 22:14:16 2012 Subject: [Histonet] Using Nail Polish on coverslip? Message-ID: <5D069EC5C4A0E743AE222EA5A02711AC298F70FF0D@NIHMLBX11.nih.gov> We are currently starting up some IHC on frozen tissue sections. After staining with different fluorescent antibodies, we end with applying DAPI w/Prolong gold and then coverslipping. We would like to seal the coverslip so that we can keep the slides longer. Any suggestions on where and how best to apply the nail polish for a permanent fix on the coverslips? Thanks, From sprice2003 <@t> gmail.com Wed Jan 4 23:16:51 2012 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Jan 4 23:16:55 2012 Subject: [Histonet] NCCI policy on IHC billing Message-ID: Histonetters: I waited a few days to see how others might weigh in after this information was posted. Call me crazy, but I expected quite a bit more reaction from our community. How is it that such a signifcant change in how IHC testing may be conducted and will be paid for in the future can produce so little response? The way this new policy is stated, it looks pretty straightfoward: one antibody (IHC procedure) per specimen; so, when it's necessary to use a battery of IHC stains to determine the origin of an undifferentiated neoplasm, the lab can only bill for one procedure. How could such an approach be possible? And what about multi-antibody procedures, which are usually more cost effective than single-antibody procedures? Come on folks, this is a big deal becuase IHC staining is essential to to the practice of anatomic pathology and provides a lot of us with our livelihood. I know I'm not alone in thinking that the CMS needs to know that this new policy is completely impractical and must be changed. Sure, there's some unnecessary IHC procedures being performed, but this isn't the way limit the problem. Sally ------------------------------ Message: 6 Date: Fri, 30 Dec 2011 12:33:17 -0600 From: "Webb, Dorothy L" Subject: [Histonet] NCCI policy update To: "'histonet@lists.utsouthwestern.edu'" Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 From Susan.Walzer <@t> HCAHealthcare.com Thu Jan 5 02:20:50 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Jan 5 02:21:04 2012 Subject: [Histonet] RE: time off In-Reply-To: <4F041200.2B7F.00C9.1@geisinger.edu> References: <6FFF9560B9CAB949BFC479164AB63A610695577C@mail1.BOSTWICK.COM> <5A33C952BB67F4468AF1F36D739212BC13AB47@JERRY.Gia.com> <3AD061FE740D464FAC7BF6B5CFB7570711F7BAC1@SMCMAIL01.somerset-healthcare.com> <4F041200.2B7F.00C9.1@geisinger.edu> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DDFAEE417@FWDCWPMSGCMS09.hca.corpad.net> Time off is one thing but holidays are another. Holidays need to be rotated. Seniority should apply to things like choice of hours but not holidays. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, January 04, 2012 8:47 AM To: Andrea; Toni Rathborne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: time off I've witnessed that granting time by seniority lets you open to abuse. I know of an employee with high seniority who takes the whole week after Christmas every year. Our policy allows only one person off per day per shift. So then no one else ever can take off at Christmastime. Her peers complain to management about it, but they won't say anything to her. Just my two cents. >>> Andrea 1/3/2012 5:40 PM >>> We also have a policy on the number of people that can be off during peak times. Our vacation schedule runs from June to June of the following year. Staff have up until a deadline of January 31 to put in their request for the next vacation year, whether it is one day or weeks. The requests handed in by this time period are granted based on seniority. ( in our institution they figure it's one of the only perks to being a senior!) After the January 31 deadline it is first come first serve. All vacation requests must be submitted on a vacation request form and time stamped when handed in. This way if two people ask for the same day the person who handed it in with an earlier time stamp is granted the time off. All of this is written in our scheduling guidelines. It seems to work pretty well. On 2012-01-03, at 4:32 PM, "Rathborne, Toni" wrote: > We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during "peak vacation time". For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, January 03, 2012 4:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] time off > > > Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Thu Jan 5 05:34:46 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Jan 5 05:34:53 2012 Subject: [Histonet] NCCI policy on IHC billing In-Reply-To: References: Message-ID: Sally, The way I read it, I don't think it's one antibody per specimen; I think what they are saying is that if you are running one antibody on multiple blocks from one specimen, you can only bill once for that antibody. I think if you are running a panel of antibodies one one block from a specimen, you can still charge for each antibody. However, I completely agree with you. This change came as a big surprise for our lab, until Dorothy posted it on Histonet we had no idea. How can that be? (And thanks, Dorothy!) The day that post came out we happened to be running 20 pankeratin/p63 double stain slides, all on multiple blocks from one specimen. The two antibodies from that double stain are applied at different times (to the same slide) and we use two different detections. So had we ran them after the new year, we would've been able to charge only once for those 20 slides, never mind 20 tests being used from each antibody and 20 tests being taken from two different detection kits. And the tech time put into cutting those 20 slides! We have several double and triple stains that we run on a daily basis, and only one component from the triple stain is a cocktail; the rest are separate antibodies being applied to the same slide using two different detections. I'm not sure exactly what our lab is going to do about it, but somehow this change should have been made aware to everyone well before it happened. There are going to be labs who are no longer in compliance, and we all know what that means.. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price [sprice2003@gmail.com] Sent: Thursday, January 05, 2012 12:16 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Histonetters: I waited a few days to see how others might weigh in after this information was posted. Call me crazy, but I expected quite a bit more reaction from our community. How is it that such a signifcant change in how IHC testing may be conducted and will be paid for in the future can produce so little response? The way this new policy is stated, it looks pretty straightfoward: one antibody (IHC procedure) per specimen; so, when it's necessary to use a battery of IHC stains to determine the origin of an undifferentiated neoplasm, the lab can only bill for one procedure. How could such an approach be possible? And what about multi-antibody procedures, which are usually more cost effective than single-antibody procedures? Come on folks, this is a big deal becuase IHC staining is essential to to the practice of anatomic pathology and provides a lot of us with our livelihood. I know I'm not alone in thinking that the CMS needs to know that this new policy is completely impractical and must be changed. Sure, there's some unnecessary IHC procedures being performed, but this isn't the way limit the problem. Sally ------------------------------ Message: 6 Date: Fri, 30 Dec 2011 12:33:17 -0600 From: "Webb, Dorothy L" Subject: [Histonet] NCCI policy update To: "'histonet@lists.utsouthwestern.edu'" Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Thu Jan 5 05:52:47 2012 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Thu Jan 5 05:52:56 2012 Subject: [Histonet] Histological mordant Message-ID: <1325764367.17100.YahooMailNeo@web29602.mail.ird.yahoo.com> Hi all, I was going to prepare a solution 1% in distlled water of? dodecaphosphomolybdic acid? as histological mordant for Mallory staining. I noticed that it was not completely soluble in water, as the Chemistry says,? but in the yellowish solution there was a thin precipitate quite heavy. So I have to filter the solution. I wonder if there will be a? leak as mordant or it will be necessary to lengthen the processing time. Any experience about? Thank you in advance. ? Kind Regards, Massimo Tosi From sfonner <@t> labpath.com Thu Jan 5 06:35:03 2012 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Thu Jan 5 06:40:55 2012 Subject: [Histonet] NCCI policy on IHC billing In-Reply-To: References: Message-ID: <001001cccba6$727fce90$577f6bb0$@com> Sally, This is a little different from what I understood yesterday. I thought that it was stated you could only bill once per part, such as an S-100 on part A,B,C would be fine, but not on A1,A3, and A5. I did not read anything about only being allowed to order one antibody. I thought panels were understood and completely acceptable as long as you were only billing for each individual stain once per part. Also, I understood the part about cocktail stains now being billed as one charge instead of multiple. Do you have a direct link to what you are stating? I mentioned it to our powers that be yesterday and they did not seem too concerned since they had heard absolutely nothing about it from our billing company. I would like to have some concrete proof before I go back to them with more bad news. Thanks, Sheila, HT (ASCP) KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Thursday, January 05, 2012 12:17 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Histonetters: I waited a few days to see how others might weigh in after this information was posted. Call me crazy, but I expected quite a bit more reaction from our community. How is it that such a signifcant change in how IHC testing may be conducted and will be paid for in the future can produce so little response? The way this new policy is stated, it looks pretty straightfoward: one antibody (IHC procedure) per specimen; so, when it's necessary to use a battery of IHC stains to determine the origin of an undifferentiated neoplasm, the lab can only bill for one procedure. How could such an approach be possible? And what about multi-antibody procedures, which are usually more cost effective than single-antibody procedures? Come on folks, this is a big deal becuase IHC staining is essential to to the practice of anatomic pathology and provides a lot of us with our livelihood. I know I'm not alone in thinking that the CMS needs to know that this new policy is completely impractical and must be changed. Sure, there's some unnecessary IHC procedures being performed, but this isn't the way limit the problem. Sally ------------------------------ Message: 6 Date: Fri, 30 Dec 2011 12:33:17 -0600 From: "Webb, Dorothy L" Subject: [Histonet] NCCI policy update To: "'histonet@lists.utsouthwestern.edu'" Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu Jan 5 07:24:10 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Thu Jan 5 07:26:33 2012 Subject: [Histonet] RE: Using Nail Polish on coverslip? In-Reply-To: <5D069EC5C4A0E743AE222EA5A02711AC298F70FF0D@NIHMLBX11.nih.gov> References: <5D069EC5C4A0E743AE222EA5A02711AC298F70FF0D@NIHMLBX11.nih.gov> Message-ID: I'll seal the coverslip around all the edges and let it dry in the hood for up to half an hour (because of the smell). I use a clear color that doesn't fluoresce under the miscroscope and also allows the slide to be stored at low temp (-20) without the nail polish seal cracking. Also be sure the polish is toluene- and benzene-free. We've discovered Revlon #771 Clear fits these parameters and works very well. Cheaper nail polishes seem to crack or flake off at cold temps. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kasai, Miki (NIH/NCI) [E] Sent: Wednesday, January 04, 2012 11:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Using Nail Polish on coverslip? We are currently starting up some IHC on frozen tissue sections. After staining with different fluorescent antibodies, we end with applying DAPI w/Prolong gold and then coverslipping. We would like to seal the coverslip so that we can keep the slides longer. Any suggestions on where and how best to apply the nail polish for a permanent fix on the coverslips? Thanks, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Genest <@t> saskatoonhealthregion.ca Thu Jan 5 07:47:03 2012 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Thu Jan 5 07:47:29 2012 Subject: [Histonet] Cutting speed Message-ID: Theresa Although I agree with the messages on not trading quality for quantity we ask our new grads to meet the following within the first 3 months. Embedding 1 min/ block Paring and cutting 2 min/block (average) Some blocks are move difficult or require more levels and will take longer and some will take less time. Can you tell me if you received any numerical responses this will give me an idea if our requirement is out of line? Thanks and good luck! Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca From raestask <@t> grics.net Thu Jan 5 07:59:41 2012 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Thu Jan 5 08:00:21 2012 Subject: [Histonet] NCCI policy on IHC billing In-Reply-To: References: Message-ID: <16B0A16CDF2F4642BC847F2AFAA1B845@your4105e587b6> Clair, I agree that the cocktailed antibodies would be charged as a single test, however, with the dual and triple stains, since these are technically done with separate procedures, would not each antibody be able to be charged? This is what we are thinking. How are others interpreting this Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Thursday, January 05, 2012 5:35 AM To: Sally Price; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Sally, The way I read it, I don't think it's one antibody per specimen; I think what they are saying is that if you are running one antibody on multiple blocks from one specimen, you can only bill once for that antibody. I think if you are running a panel of antibodies one one block from a specimen, you can still charge for each antibody. However, I completely agree with you. This change came as a big surprise for our lab, until Dorothy posted it on Histonet we had no idea. How can that be? (And thanks, Dorothy!) The day that post came out we happened to be running 20 pankeratin/p63 double stain slides, all on multiple blocks from one specimen. The two antibodies from that double stain are applied at different times (to the same slide) and we use two different detections. So had we ran them after the new year, we would've been able to charge only once for those 20 slides, never mind 20 tests being used from each antibody and 20 tests being taken from two different detection kits. And the tech time put into cutting those 20 slides! We have several double and triple stains that we run on a daily basis, and only one component from the triple stain is a cocktail; the rest are separate antibodies being applied to the same slide using two different detections. I'm not sure exactly what our lab is going to do about it, but somehow this change should have been made aware to everyone well before it happened. There are going to be labs who are no longer in compliance, and we all know what that means.. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price [sprice2003@gmail.com] Sent: Thursday, January 05, 2012 12:16 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Histonetters: I waited a few days to see how others might weigh in after this information was posted. Call me crazy, but I expected quite a bit more reaction from our community. How is it that such a signifcant change in how IHC testing may be conducted and will be paid for in the future can produce so little response? The way this new policy is stated, it looks pretty straightfoward: one antibody (IHC procedure) per specimen; so, when it's necessary to use a battery of IHC stains to determine the origin of an undifferentiated neoplasm, the lab can only bill for one procedure. How could such an approach be possible? And what about multi-antibody procedures, which are usually more cost effective than single-antibody procedures? Come on folks, this is a big deal becuase IHC staining is essential to to the practice of anatomic pathology and provides a lot of us with our livelihood. I know I'm not alone in thinking that the CMS needs to know that this new policy is completely impractical and must be changed. Sure, there's some unnecessary IHC procedures being performed, but this isn't the way limit the problem. Sally ------------------------------ Message: 6 Date: Fri, 30 Dec 2011 12:33:17 -0600 From: "Webb, Dorothy L" Subject: [Histonet] NCCI policy update To: "'histonet@lists.utsouthwestern.edu'" Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Thu Jan 5 08:12:23 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Jan 5 08:12:31 2012 Subject: [Histonet] decal solution for molecular studies Message-ID: Does anyone know of a decal solution that enables molecular studies (Her 2 FISH, EGFR by PCR) to be performed later? We use Formical for our decal solution. Her 2 FISH works about 50% of the time, EGFR almost never. We are looking at having to cut undecalcified bone today for both these studies, and we are not equipped to cut undecalcified bone. Any suggestions? Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From mward <@t> wakehealth.edu Thu Jan 5 08:30:59 2012 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Jan 5 08:31:19 2012 Subject: [Histonet] NCCI policy on IHC billing In-Reply-To: <16B0A16CDF2F4642BC847F2AFAA1B845@your4105e587b6> References: <16B0A16CDF2F4642BC847F2AFAA1B845@your4105e587b6> Message-ID: >From what we understand from the new policy the cocktailed antibodies, such as PIN4, are not to be charged separately. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Thursday, January 05, 2012 9:00 AM To: 'Clare Thornton'; 'Sally Price'; histonet@lists.utsouthwestern.edu Cc: Dana Spears Subject: RE: [Histonet] NCCI policy on IHC billing Clair, I agree that the cocktailed antibodies would be charged as a single test, however, with the dual and triple stains, since these are technically done with separate procedures, would not each antibody be able to be charged? This is what we are thinking. How are others interpreting this Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Thursday, January 05, 2012 5:35 AM To: Sally Price; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Sally, The way I read it, I don't think it's one antibody per specimen; I think what they are saying is that if you are running one antibody on multiple blocks from one specimen, you can only bill once for that antibody. I think if you are running a panel of antibodies one one block from a specimen, you can still charge for each antibody. However, I completely agree with you. This change came as a big surprise for our lab, until Dorothy posted it on Histonet we had no idea. How can that be? (And thanks, Dorothy!) The day that post came out we happened to be running 20 pankeratin/p63 double stain slides, all on multiple blocks from one specimen. The two antibodies from that double stain are applied at different times (to the same slide) and we use two different detections. So had we ran them after the new year, we would've been able to charge only once for those 20 slides, never mind 20 tests being used from each antibody and 20 tests being taken from two different detection kits. And the tech time put into cutting those 20 slides! We have several double and triple stains that we run on a daily basis, and only one component from the triple stain is a cocktail; the rest are separate antibodies being applied to the same slide using two different detections. I'm not sure exactly what our lab is going to do about it, but somehow this change should have been made aware to everyone well before it happened. There are going to be labs who are no longer in compliance, and we all know what that means.. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price [sprice2003@gmail.com] Sent: Thursday, January 05, 2012 12:16 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Histonetters: I waited a few days to see how others might weigh in after this information was posted. Call me crazy, but I expected quite a bit more reaction from our community. How is it that such a signifcant change in how IHC testing may be conducted and will be paid for in the future can produce so little response? The way this new policy is stated, it looks pretty straightfoward: one antibody (IHC procedure) per specimen; so, when it's necessary to use a battery of IHC stains to determine the origin of an undifferentiated neoplasm, the lab can only bill for one procedure. How could such an approach be possible? And what about multi-antibody procedures, which are usually more cost effective than single-antibody procedures? Come on folks, this is a big deal becuase IHC staining is essential to to the practice of anatomic pathology and provides a lot of us with our livelihood. I know I'm not alone in thinking that the CMS needs to know that this new policy is completely impractical and must be changed. Sure, there's some unnecessary IHC procedures being performed, but this isn't the way limit the problem. Sally ------------------------------ Message: 6 Date: Fri, 30 Dec 2011 12:33:17 -0600 From: "Webb, Dorothy L" Subject: [Histonet] NCCI policy update To: "'histonet@lists.utsouthwestern.edu'" Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Thu Jan 5 09:16:06 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Jan 5 09:16:16 2012 Subject: [Histonet] RE: decal solution for molecular studies In-Reply-To: References: Message-ID: To clarify, the Formical we use is a formic acid/EDTA solution. Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Thursday, January 05, 2012 9:12 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] decal solution for molecular studies Does anyone know of a decal solution that enables molecular studies (Her 2 FISH, EGFR by PCR) to be performed later? We use Formical for our decal solution. Her 2 FISH works about 50% of the time, EGFR almost never. We are looking at having to cut undecalcified bone today for both these studies, and we are not equipped to cut undecalcified bone. Any suggestions? Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Thu Jan 5 09:59:35 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Jan 5 09:59:46 2012 Subject: [Histonet] Amazing Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From jqb7 <@t> cdc.gov Thu Jan 5 10:08:36 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Jan 5 10:08:40 2012 Subject: [Histonet] RE: Amazing In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> Message-ID: If you are talking about a regressive H&E stain then perhaps it was 1% HCL acid in 70% alcohol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, January 05, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amazing I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 5 10:16:48 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 5 10:16:54 2012 Subject: [Histonet] Amazing In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> Message-ID: <1325780208.90675.YahooMailClassic@web65703.mail.ac4.yahoo.com> You can use either acetic or hydrochloric, both will do. The strength is 1% BUT it does not matter. As a matter of fact the weaker the solution the better. Remember that the acid solution (that can be made with 7ethanolanol even better) is used to differentiate progressivesive hematoxylin, like Harris, and the weaker it is the more control you have with the differentiation. A weak solution allows you to dip the slides several times until you obtain the desired reddish hue on the sections that signals when the differentiation is completed. If the solution is too strong you will have to take the slides often precipitouslyusly and some sections will be have a weak nuclear staining. So, use acetic at 1% in 70% ethanol? That is what I used to prepare. Ren? J. --- On Thu, 1/5/12, Sarah Dysart wrote: From: Sarah Dysart Subject: [Histonet] Amazing To: "histonet@lists.utsouthwestern.edu" Date: Thursday, January 5, 2012, 10:59 AM I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.? That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).? I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.? I want to say it was like a 1% acid solution in alcohol??? What was the acid?? For some reason my brain says glacial acetic...but time has made me forget.? Is the alcohol you mix it in 100% or something lower with a water content to it?? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Jan 5 10:24:51 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jan 5 10:24:55 2012 Subject: [Histonet] Amazing In-Reply-To: <1325780208.90675.YahooMailClassic@web65703.mail.ac4.yahoo.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> <1325780208.90675.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E19DC42@evcspmbx3.ads.northwestern.edu> We use .5% acid alcohol ( hydrochloric) with Harris hemo. No problems. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 05, 2012 10:17 AM To: histonet@lists.utsouthwestern.edu; Sarah Dysart Subject: Re: [Histonet] Amazing You can use either acetic or hydrochloric, both will do. The strength is 1% BUT it does not matter. As a matter of fact the weaker the solution the better. Remember that the acid solution (that can be made with 7ethanolanol even better) is used to differentiate progressivesive hematoxylin, like Harris, and the weaker it is the more control you have with the differentiation. A weak solution allows you to dip the slides several times until you obtain the desired reddish hue on the sections that signals when the differentiation is completed. If the solution is too strong you will have to take the slides often precipitouslyusly and some sections will be have a weak nuclear staining. So, use acetic at 1% in 70% ethanol? That is what I used to prepare. Ren? J. --- On Thu, 1/5/12, Sarah Dysart wrote: From: Sarah Dysart Subject: [Histonet] Amazing To: "histonet@lists.utsouthwestern.edu" Date: Thursday, January 5, 2012, 10:59 AM I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.? That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).? I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.? I want to say it was like a 1% acid solution in alcohol??? What was the acid?? For some reason my brain says glacial acetic...but time has made me forget.? Is the alcohol you mix it in 100% or something lower with a water content to it?? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Thu Jan 5 10:25:27 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Jan 5 10:26:21 2012 Subject: [Histonet] RE: Amazing In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC006483C0722@IL084EXMBX214.illinois.gov> I still use that. It is 70% with 5mL of hydrochloric acid if making 1L. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [sdysart@mirnarx.com] Sent: Thursday, January 05, 2012 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amazing I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Thu Jan 5 11:16:43 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Jan 5 11:16:57 2012 Subject: [Histonet] Look at me... Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D509C9831@SN2PRD0702MB098.namprd07.prod.outlook.com> I'm just full of questions today!! This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. I do all the blocking including Fc receptors...still junk. The clone I have been using is, from abcam (ab2302). I don't see the specific clone name listed. I am staining human xenografts raised in mouse. I get a whole lot of background staining making it very hard to find the positive staining. The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. At the higher dilution no positive staining or background is observed. Does anyone know of a good Caspase3 antibody, preferably mouse monoclonal? All the rabbit polyclonal antibodies are difficult to stain on these xenografts. Thanks again =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From gayle.callis <@t> bresnan.net Thu Jan 5 11:35:20 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Jan 5 11:35:43 2012 Subject: [Histonet] Nail Polish sealant Message-ID: <000e01cccbd0$67966f50$36c34df0$@bresnan.net> You wrote: We are currently starting up some IHC on frozen tissue sections. After staining with different fluorescent antibodies, we end with applying DAPI w/Prolong gold and then coverslipping. We would like to seal the coverslip so that we can keep the slides longer. Any suggestions on where and how best to apply the nail polish for a permanent fix on the coverslips? ***************************** Prolong Gold antifade reagent is a hard seal to begin with. We only seal ends but not the sides of cover glass. Our coverslips go right to edge of slides e.g. 25 X 30, 25 x 40, or 25 X 50. We don't like having nail polish slop over the edges onto back of slides but one could seal all sides of coverslip if careful. We buy the thinner top or base coat nail polish, but in general, prefer to use thinned mounting media rather than nail polish. There can be some issues here. If you are trying to view GFP or RFP (red fluorescence protein) labeled cells or tissue components, you should not seal the coverslip with nail polish since the alcohol in nail polish leaches under the coverslip and causes GFP/RFP to fade. This fact was published in Science. We found that dumping out cheap clear nail polish from bottle, rinsing away the residue with acetone, and then adding permanent mounting media and thinning that with toluene to the consistency of top coat nail polish works best. Toluene or xylene based sealants cannot leach under the cover glass since these solvents are NOT miscible with water in the PBS. Thinned mounting media is better sealant for GFP purposes (no fading) and also works for IF stained sections (perfect seal). We love the little brush in the nail polish bottle for application. Thicker clear nail polish (for non GFP studies) or IF stained sections is messy during application so we buy the cheapest top coat polish we can find at Walmart. DAPI in the Prolong Gold will cause an uneven staining gradient so that some of the nuclei in the center of a section are not stained as brightly as the nuclei on the outer edges of a stained section. The cause is not getting enough thicker Prolong Gold/DAPI over the section or not having just the right amount of buffer on the section to permit a good flow of this wonderful mounting media over the section. We now complete all IF staining then stain with a DAPI solution before cover slipping with Prolong Gold. You can buy ready to use DAPI solutions from Pierce or Biogenex, or make up the solution in house. You can find the recipe at IHCworld website or simply Google. We do NOT store our IF stained slides in the cold, but in a folder at RT in a dark drawer before viewing on the day after staining to reduce any movement/flow under the coverslip. Fluorophores can and will eventually fade. I do not recall any studies saying storing IF stained slides in the cold reduces fading but we never have space to do cold storage anyway and store slides at RT. The new fluorophores (Alexas and Dylights) remain stable over a longer time even for several weeks compared to fluorescein derivatives e.g. FITC TRITC. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From liz <@t> premierlab.com Thu Jan 5 11:36:16 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 5 11:36:21 2012 Subject: [Histonet] RE: Look at me... In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D509C9831@SN2PRD0702MB098.namprd07.prod.outlook.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC7447@SBS2K8.premierlab.local> If you are looking to stain cleaved caspase 3 there is a better antibody from cell signaling it works in multiple species, it's a bit pricey but I have found that it works the best out of the few that I have tried. We have used it mouse xenografts before without any issue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, January 05, 2012 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Look at me... I'm just full of questions today!! This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. I do all the blocking including Fc receptors...still junk. The clone I have been using is, from abcam (ab2302). I don't see the specific clone name listed. I am staining human xenografts raised in mouse. I get a whole lot of background staining making it very hard to find the positive staining. The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. At the higher dilution no positive staining or background is observed. Does anyone know of a good Caspase3 antibody, preferably mouse monoclonal? All the rabbit polyclonal antibodies are difficult to stain on these xenografts. Thanks again =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Jan 5 11:45:25 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jan 5 11:45:39 2012 Subject: [Histonet] Elastichrome paper, and troubleshooting Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE62C@MCINFRWEM003.ucsfmedicalcenter.org> Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain: Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975 We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past. I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible. Our problem is that the elastic stain washes out during or after the trichrome. Procedure: Bouins, 56C one hour Wiegerts hematolylin, 3min Verhoffs elastic stain, 15 min 2% ferric chloride differentiation (so far so-good) Gomori's trichrome Blue, 15 min 0.2% glacial acetic acid, 1 min Wash dH20, dehydrate, clear, mount Results: trichrome looks great, no elastin stain. I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions. Thanks for any help. Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From liz <@t> premierlab.com Thu Jan 5 11:50:05 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 5 11:50:12 2012 Subject: [Histonet] RE: Elastichrome paper, and troubleshooting In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE62C@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC7449@SBS2K8.premierlab.local> Tim We do this stain all of the time, we never used the original reference we just made up one on our own. I'll send our SOP in a different e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, January 05, 2012 10:45 AM To: Histonet Subject: [Histonet] Elastichrome paper, and troubleshooting Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain: Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975 We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past. I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible. Our problem is that the elastic stain washes out during or after the trichrome. Procedure: Bouins, 56C one hour Wiegerts hematolylin, 3min Verhoffs elastic stain, 15 min 2% ferric chloride differentiation (so far so-good) Gomori's trichrome Blue, 15 min 0.2% glacial acetic acid, 1 min Wash dH20, dehydrate, clear, mount Results: trichrome looks great, no elastin stain. I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions. Thanks for any help. Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Jan 5 12:04:32 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jan 5 12:04:38 2012 Subject: [Histonet] RE: Look at me... In-Reply-To: <14E2C6176416974295479C64A11CB9AE011380AC7447@SBS2K8.premierlab.local> References: <8A70A9B2ECDD084DACFE6C59FCF86D509C9831@SN2PRD0702MB098.namprd07.prod.outlook.com> <14E2C6176416974295479C64A11CB9AE011380AC7447@SBS2K8.premierlab.local> Message-ID: I'll second that from our experience - Cell Signaling cleaved caspase-3 antibody works well on xenografts Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, January 05, 2012 12:36 PM To: 'Sarah Dysart'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Look at me... If you are looking to stain cleaved caspase 3 there is a better antibody from cell signaling it works in multiple species, it's a bit pricey but I have found that it works the best out of the few that I have tried. We have used it mouse xenografts before without any issue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, January 05, 2012 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Look at me... I'm just full of questions today!! This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. I do all the blocking including Fc receptors...still junk. The clone I have been using is, from abcam (ab2302). I don't see the specific clone name listed. I am staining human xenografts raised in mouse. I get a whole lot of background staining making it very hard to find the positive staining. The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. At the higher dilution no positive staining or background is observed. Does anyone know of a good Caspase3 antibody, preferably mouse monoclonal? All the rabbit polyclonal antibodies are difficult to stain on these xenografts. Thanks again =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From TNMayer <@t> mdanderson.org Thu Jan 5 12:44:27 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Jan 5 12:44:31 2012 Subject: [Histonet] RE: In-Reply-To: <84f97187-ebe3-49ec-a407-486409ae16d2@DCPWPRTR03.mdanderson.edu> References: <84f97187-ebe3-49ec-a407-486409ae16d2@DCPWPRTR03.mdanderson.edu> Message-ID: Sarah, I would use the 1% Acetic Acid in 70% alcohol, for a couple of reasons: your docs are used to looking at the stain with the Clarifier, and acetic is the ingredient in the Clarifier 2; also you probably will not have to change your staining times by too much if you use acetic. You could probably even dilute it out to 50% alcohol. HCL works too fast, especially since I have students. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ Message: 2 Date: Thu, 5 Jan 2012 15:59:35 +0000 From: Sarah Dysart Subject: [Histonet] Amazing To: "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ****************************** From TNMayer <@t> mdanderson.org Thu Jan 5 12:56:48 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Jan 5 12:56:54 2012 Subject: [Histonet] RE: Elastichrome Paper In-Reply-To: <84f97187-ebe3-49ec-a407-486409ae16d2@DCPWPRTR03.mdanderson.edu> References: <84f97187-ebe3-49ec-a407-486409ae16d2@DCPWPRTR03.mdanderson.edu> Message-ID: Tim, I do not have the paper, but a few years ago I worked the kinks out of that stain. Your acetic acid is too long. Try for the amount of time that you usually do for a one step. A few dips would be a good start. Then rinse in dH2O, not wash. Also, I do not remember using the Weigert's and Verhoeff's solution. No need. Just the Bouins mordant, VVG and did a trichrome as a counter stain. Also, do not differentiate as much with the Ferric. Under differentiation will compensate for the other reagents. How long do you usually leave the slides in one step when doing a regular one? Those would be good starting points. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ Message: 10 Date: Thu, 5 Jan 2012 09:45:25 -0800 From: "Morken, Timothy" Subject: [Histonet] Elastichrome paper, and troubleshooting To: Histonet Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE62C@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain: Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975 We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past. I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible. Our problem is that the elastic stain washes out during or after the trichrome. Procedure: Bouins, 56C one hour Wiegerts hematolylin, 3min Verhoffs elastic stain, 15 min 2% ferric chloride differentiation (so far so-good) Gomori's trichrome Blue, 15 min 0.2% glacial acetic acid, 1 min Wash dH20, dehydrate, clear, mount Results: trichrome looks great, no elastin stain. I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions. Thanks for any help. Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org ************************************* From beth.villarreal <@t> novartis.com Thu Jan 5 14:02:21 2012 From: beth.villarreal <@t> novartis.com (Villarreal, Beth) Date: Thu Jan 5 14:02:27 2012 Subject: [Histonet] saffron vs. safran du gatinais Message-ID: Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA From ASelf <@t> georgetownhospitalsystem.org Thu Jan 5 14:09:05 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Thu Jan 5 14:09:17 2012 Subject: [Histonet] Respirators and Routine Histology Message-ID: Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From jag93 <@t> cam.ac.uk Thu Jan 5 14:18:06 2012 From: jag93 <@t> cam.ac.uk (Andrew Gillis) Date: Thu Jan 5 14:18:14 2012 Subject: [Histonet] Clearing NBT/BCIP precipitate from wax sections Message-ID: <4F06057E.6090005@cam.ac.uk> Hello, I have some paraffin sections that I've used for immunohistochemistry (using an alkaline phosphatase-conjugated secondary antibody and NBT/BCIP as a substrate), and coverslipped with an aqueous mounting medium (Fluoromount G). I would now like to de-coverslip and re-stain these sections with a different histochemical protocol (i.e. Masson's trichrome or H&E). Does anybody know if there is a way to "clear away" the colour reaction precipitate from my previous IHC? If, after decoverslipping, I dehydrate and then rehydrate the sections, will this do the job? Any advice would be greatly appreciated. Thanks very much, Andrew From lisab <@t> hollandhospital.org Thu Jan 5 14:22:21 2012 From: lisab <@t> hollandhospital.org (Lisa Brenner) Date: Thu Jan 5 14:22:30 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: References: Message-ID: <4F05C02D0200003C0000908D@gwapp03p> No, not here. We don't wear anything at all. We have excellent air flow, and we wear the badges that test for exposure to xylene and formalin annually which I think is a CAP requirement. Our results have been better than acceptable every year. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org>>> Amy Self 1/5/2012 3:09 PM >>> Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From lblazek <@t> digestivespecialists.com Thu Jan 5 14:30:20 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 5 14:29:12 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: <4F05C02D0200003C0000908D@gwapp03p> References: <4F05C02D0200003C0000908D@gwapp03p> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA01FF8@IBMB7Exchange.digestivespecialists.com> Nothing at all??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, January 05, 2012 3:22 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Respirators and Routine Histology No, not here. We don't wear anything at all. We have excellent air flow, and we wear the badges that test for exposure to xylene and formalin annually which I think is a CAP requirement. Our results have been better than acceptable every year. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org>>> Amy Self 1/5/2012 3:09 PM >>> Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsjurczak <@t> comcast.net Thu Jan 5 14:31:17 2012 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Thu Jan 5 14:31:40 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39137DA01FF8@IBMB7Exchange.digestivespecialists.com> Message-ID: <730597643.370044.1325795477561.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> They must be in a liberal part of Michigan. ----- Original Message ----- From: "Linda Blazek" To: "Lisa Brenner" , "Amy Self" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 5, 2012 2:30:20 PM Subject: RE: [Histonet] Respirators and Routine Histology Nothing at all??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, January 05, 2012 3:22 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Respirators and Routine Histology No, not here. We don't wear anything at all. We have excellent air flow, and we wear the badges that test for exposure to xylene and formalin annually which I think is a CAP requirement. Our results have been better than acceptable every year. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org>>> Amy Self 1/5/2012 3:09 PM >>> Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Thu Jan 5 15:14:01 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Thu Jan 5 15:14:04 2012 Subject: [Histonet] saffron vs. safran du gatinais In-Reply-To: References: Message-ID: Hi Beth, "Saffron" and "safran du gatinais" both refer to the dried stigma of the saffron crocus. They are both "saffron." I use and alcoholic extract of Saffron to stain collagen in Movat stains. I've tried saffron from multiple sources and price definitely does not always correlate with quality. I gave up on Sigma-Aldrich, for example. The best saffron has a deep red color and is highly aromatic. There are possible workarounds for the lower quality material--like use more saffron, or stain longer, but best to use the best. What stain are you doing and what solution are you making with the saffron? Jerry Ricks Research Scientist University of Washington Department of Pathology > From: beth.villarreal@novartis.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 5 Jan 2012 20:02:21 +0000 > Subject: [Histonet] saffron vs. safran du gatinais > > Hello histonet, > I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? > > Many thanks, > Beth > > > Beth Villarreal > Scientist I > Novartis Institutes for BioMedical > Research, Inc. > 300 Technology Square > Cambridge, MA 02139 > USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jan 5 15:34:33 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 5 15:34:37 2012 Subject: [Histonet] antibody to CpiV Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC7464@SBS2K8.premierlab.local> Is anyone aware of a source for an antibody to CpiV - canine parainfluenza virus. I have searched and come across some papers with IHC staining but I am unable to access the complete paper. Any help would be appreciated. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 From ttruscot <@t> vetmed.wsu.edu Thu Jan 5 15:54:45 2012 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jan 5 15:56:03 2012 Subject: [Histonet] RE: saffron vs. safran du gatinais In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A002718821133@CVM76.vetmed.wsu.edu> Hi Beth, Saffron is a great spice. We use it in a old family recipe from Cornwall for a bread roll( saffron nubbies). We also find the price very expensive, but also varies a lot. If you know anyone in the middle East, or India, they could get it a lot cheaper. We have raised it in our garden and get about four 3/4 in long stigmas from each flower, so it is labor intensive to get much, but a little goes a long way. It has to. I don't know of any substitute in staining. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Villarreal, Beth Sent: Thursday, January 05, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Thu Jan 5 16:46:42 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Thu Jan 5 15:58:53 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: References: Message-ID: <4608.208.62.167.196.1325803602.squirrel@webmail.realpages.com> I also do not wear any type of respirator. Not at any point of my day. I annually wear a formalin badge to test for exposure rate, but thats it. I gross under a fume hood and I use Slide Bright instead of xylene. It does not have any fumes or noxious odor and is non toxic. My stain line is also contained under a fume hood. No one ever smells or complains about my fumes, unless im changing the processor, they tend to smell the alcohol then. Mask are a required safety supply, and I do believe in some situations a respirator may be needed, but it is ultimately up to the tech. Besides the lab should have adequite ventilation that a respirator should not be needed for the entire shift, maybe during specfic tasks with high fumes. Nicole Tatum, HT ASCP Happy New Year to All, > > I need some help from all of you out there in histoland. > > How many of you wear respirators during your entire 8 hour work day for > routine histology? If you don't wear a respirator do you wear any type of > mask or shield at all for routine histology? > > Also if any of you have any histology safety procedures or information > that you would be willing to share with me I would greatly appreciate it. > > Thanks in advance for all of your help, Amy > > > Amy Self > Georgetown Hospital System > 843-527-7179 > NOTE: > The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify us immediately by replying to this message and deleting it from > your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From renafail2 <@t> gmail.com Thu Jan 5 16:10:17 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Thu Jan 5 16:10:21 2012 Subject: [Histonet] RE: Amazing In-Reply-To: <4A6E2CACA1E017408EBA1B9911952CC006483C0722@IL084EXMBX214.illinois.gov> References: <8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> <4A6E2CACA1E017408EBA1B9911952CC006483C0722@IL084EXMBX214.illinois.gov> Message-ID: 0.5% HCL acid ih 70 % ethanol Rena fail On Thu, Jan 5, 2012 at 11:25 AM, Burton, Lynn wrote: > I still use that. It is 70% with 5mL of hydrochloric acid if making 1L. > Lynn Burton > Lab Assoc I > Animal Disease Lab > Galesburg, Il > 309-344-2451 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [ > sdysart@mirnarx.com] > Sent: Thursday, January 05, 2012 9:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amazing > > I find it amazing sometimes when you don't do something for awhile how > quickly your brain throws the information away. That being said...I know > back in the day when I was learning histology we used to make our own acid > alcohol solution (now where I am had a butt load of Clearifier so I was > using that up). I don't want to buy that stuff anymore as making the > solution is way cheaper and works just as well. I want to say it was like > a 1% acid solution in alcohol?? What was the acid? For some reason my > brain says glacial acetic...but time has made me forget. Is the alcohol > you mix it in 100% or something lower with a water content to it? Please > help my alzheimers =) > Happy Thursday!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sdysart <@t> mirnarx.com Thu Jan 5 16:11:14 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Jan 5 16:11:20 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: <730597643.370044.1325795477561.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <5A2BD13465E061429D6455C8D6B40E39137DA01FF8@IBMB7Exchange.digestivespecialists.com> <730597643.370044.1325795477561.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D509C9964@SN2PRD0702MB098.namprd07.prod.outlook.com> I'm in Texas and have been in histology labs since 1998 in 4 different places. None of them did we ever wear a respirator for normal histology work. We did have to wear masks when cutting frozens because of possible TB in lung tissue, but that was it. Most labs should have some kind of negative pressure in them and be exhausting out of the room. I also have always had some kind of hood over the staining station, and usually another hood to gross specimens in. I would say wearing a respirator every day for 8 hours would have made me decide on another field!! How irritating!! Good Luck! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net Sent: Thursday, January 05, 2012 2:31 PM To: Linda Blazek Cc: histonet@lists.utsouthwestern.edu; Amy Self Subject: Re: [Histonet] Respirators and Routine Histology They must be in a liberal part of Michigan. ----- Original Message ----- From: "Linda Blazek" To: "Lisa Brenner" , "Amy Self" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 5, 2012 2:30:20 PM Subject: RE: [Histonet] Respirators and Routine Histology Nothing at all??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, January 05, 2012 3:22 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Respirators and Routine Histology No, not here. We don't wear anything at all. We have excellent air flow, and we wear the badges that test for exposure to xylene and formalin annually which I think is a CAP requirement. Our results have been better than acceptable every year. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org>>> Amy Self 1/5/2012 3:09 PM >>> Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Jan 5 16:17:44 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 5 16:17:53 2012 Subject: [Histonet] RE: Elastichrome paper, and troubleshooting In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE62C@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89F5EE62C@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A0F3E3@xmdb02.nch.kids> How old is the alcoholic Hx you use to prepare the Verhoeffs. I have found that matured 10% ethanoic Hx is more resistant to differentiation than freshly prepared Hx. Might be of use Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, 6 January 2012 4:45 AM To: Histonet Subject: [Histonet] Elastichrome paper, and troubleshooting Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain: Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975 We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past. I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible. Our problem is that the elastic stain washes out during or after the trichrome. Procedure: Bouins, 56C one hour Wiegerts hematolylin, 3min Verhoffs elastic stain, 15 min 2% ferric chloride differentiation (so far so-good) Gomori's trichrome Blue, 15 min 0.2% glacial acetic acid, 1 min Wash dH20, dehydrate, clear, mount Results: trichrome looks great, no elastin stain. I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions. Thanks for any help. Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From gayle.callis <@t> bresnan.net Thu Jan 5 16:21:04 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Jan 5 16:21:23 2012 Subject: [Histonet] saffron vs. safran du gatinais In-Reply-To: References: Message-ID: <000301cccbf8$51f9b8f0$f5ed2ad0$@bresnan.net> Beth, In the past, people replied to Histonet with the suggestion to buy saffron aka safran du gatinais from a grocery store spice section. Depending on where you are located e.g. a bigger city, try to find a store that sells spices from India may have the freshest saffron. However, you won't suffer the sticker shock of buying it from a chemical supplier. It still tends to be expensive but not like chemical company prices. Once we made the alcoholic saffron solution for Movat's pentachrome, we stored this solution in a container with a dessicant to maintain a water free environment. I was fascinated that Tom actually grew and harvested saffron from the flowers. That is true devotion, but I suspect it was for those delicious sounding "nubbies . Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Villarreal, Beth Sent: Thursday, January 05, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Fri Jan 6 00:12:40 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Jan 6 00:12:47 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 6 In-Reply-To: <4f05e540.06a1ec0a.7315.ffffabc8SMTPIN_ADDED@mx.google.com> References: <4f05e540.06a1ec0a.7315.ffffabc8SMTPIN_ADDED@mx.google.com> Message-ID: Sarah, Your background is caused by cross-activity from your mouse primary antibody on mouse tissue (xenografts). You can try Mouse on Mouse kit (Biocare's has a great Mouse on Mouse HRP Polymer system). I have used Cell Signaling's Rabbit anti-Cleaved Caspase 3 (Cat # 9661) on Xenografts and they work very well. They are very specified and high affinity (appx. ~ 1:10k) with Dako's Envision + system. Your problem will be solve if you just switch your primary ab from mouse to rabbit. Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleinehuey@elcaminohospital.org On Thu, Jan 5, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. RE: decal solution for molecular studies (Clare Thornton) > ? 2. Amazing (Sarah Dysart) > ? 3. RE: Amazing (Bartlett, Jeanine (CDC/OID/NCEZID)) > ? 4. Re: Amazing (Rene J Buesa) > ? 5. RE: Amazing (Bernice Frederick) > ? 6. RE: Amazing (Burton, Lynn) > ? 7. Look at me... (Sarah Dysart) > ? 8. Nail Polish sealant (gayle callis) > ? 9. RE: Look at me... (Elizabeth Chlipala) > ?10. Elastichrome paper, and troubleshooting (Morken, Timothy) > ?11. RE: Elastichrome paper, and troubleshooting (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Jan 2012 10:16:06 -0500 > From: Clare Thornton > Subject: [Histonet] RE: decal solution for molecular studies > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > To clarify, the Formical we use is a formic acid/EDTA solution. > > > Clare J. Thornton, HTL(ASCP), QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton > Sent: Thursday, January 05, 2012 9:12 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] decal solution for molecular studies > > Does anyone know of a decal solution that enables molecular studies (Her 2 FISH, EGFR by PCR) to be performed later? ?We use Formical for our decal solution. ?Her 2 FISH works about 50% of the time, EGFR almost never. ?We are looking at having to cut undecalcified bone today for both these studies, and we are not equipped to cut undecalcified bone. ?Any suggestions? > > > > Clare J. Thornton, HTL(ASCP), QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Thu, 5 Jan 2012 15:59:35 +0000 > From: Sarah Dysart > Subject: [Histonet] Amazing > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<8A70A9B2ECDD084DACFE6C59FCF86D509C9766@SN2PRD0702MB098.namprd07.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. ?That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). ?I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. ?I want to say it was like a 1% acid solution in alcohol?? ?What was the acid? ?For some reason my brain says glacial acetic...but time has made me forget. ?Is the alcohol you mix it in 100% or something lower with a water content to it? ?Please help my alzheimers =) > Happy Thursday!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas ?78744 > (512)901-0900 ext. 6912 > > > > ------------------------------ > > Message: 3 > Date: Thu, 5 Jan 2012 16:08:36 +0000 > From: "Bartlett, Jeanine (CDC/OID/NCEZID)" > Subject: [Histonet] RE: Amazing > To: Sarah Dysart , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > If you are talking about a regressive H&E stain then perhaps it was 1% HCL acid in 70% alcohol > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart > Sent: Thursday, January 05, 2012 11:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amazing > > I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. ?That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). ?I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. ?I want to say it was like a 1% acid solution in alcohol?? ?What was the acid? ?For some reason my brain says glacial acetic...but time has made me forget. ?Is the alcohol you mix it in 100% or something lower with a water content to it? ?Please help my alzheimers =) Happy Thursday!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas ?78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Thu, 5 Jan 2012 08:16:48 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Amazing > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Sarah Dysart > ? ? ? ? > Message-ID: > ? ? ? ?<1325780208.90675.YahooMailClassic@web65703.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > You can use either acetic or hydrochloric, both will do. The strength is 1% BUT it does not matter. As a matter of fact the weaker the solution the better. Remember that the acid solution (that can be made with 7ethanolanol even better) is used to differentiate progressivesive hematoxylin, like Harris, and the weaker it is the more control you have with the differentiation. > A weak solution allows you to dip the slides several times until you obtain the desired reddish hue on the sections that signals when the differentiation is completed. > If the solution is too strong you will have to take the slides often precipitouslyusly and some sections will be have a weak nuclear staining. > So, use acetic at 1% in 70% ethanol? That is what I used to prepare. > Ren? J. > > --- On Thu, 1/5/12, Sarah Dysart wrote: > > > From: Sarah Dysart > Subject: [Histonet] Amazing > To: "histonet@lists.utsouthwestern.edu" > Date: Thursday, January 5, 2012, 10:59 AM > > > I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.? That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).? I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.? I want to say it was like a 1% acid solution in alcohol??? What was the acid?? For some reason my brain says glacial acetic...but time has made me forget.? Is the alcohol you mix it in 100% or something lower with a water content to it?? Please help my alzheimers =) > Happy Thursday!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas? 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Thu, 5 Jan 2012 16:24:51 +0000 > From: Bernice Frederick > Subject: RE: [Histonet] Amazing > To: Rene J Buesa , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ?, Sarah Dysart > ? ? ? ? > Message-ID: > ? ? ? ?<62C639732D3F274DACED033EBDF6ADAF1E19DC42@evcspmbx3.ads.northwestern.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > We use .5% ?acid alcohol ( hydrochloric) with Harris hemo. No problems. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, January 05, 2012 10:17 AM > To: histonet@lists.utsouthwestern.edu; Sarah Dysart > Subject: Re: [Histonet] Amazing > > You can use either acetic or hydrochloric, both will do. The strength is 1% BUT it does not matter. As a matter of fact the weaker the solution the better. Remember that the acid solution (that can be made with 7ethanolanol even better) is used to differentiate progressivesive hematoxylin, like Harris, and the weaker it is the more control you have with the differentiation. > A weak solution allows you to dip the slides several times until you obtain the desired reddish hue on the sections that signals when the differentiation is completed. > If the solution is too strong you will have to take the slides often precipitouslyusly and some sections will be have a weak nuclear staining. > So, use acetic at 1% in 70% ethanol? That is what I used to prepare. > Ren? J. > > --- On Thu, 1/5/12, Sarah Dysart wrote: > > > From: Sarah Dysart > Subject: [Histonet] Amazing > To: "histonet@lists.utsouthwestern.edu" > Date: Thursday, January 5, 2012, 10:59 AM > > > I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.? That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).? I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.? I want to say it was like a 1% acid solution in alcohol??? What was the acid?? For some reason my brain says glacial acetic...but time has made me forget.? Is the alcohol you mix it in 100% or something lower with a water content to it?? Please help my alzheimers =) Happy Thursday!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas? 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Thu, 5 Jan 2012 10:25:27 -0600 > From: "Burton, Lynn" > Subject: [Histonet] RE: Amazing > To: Sarah Dysart , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<4A6E2CACA1E017408EBA1B9911952CC006483C0722@IL084EXMBX214.illinois.gov> > > Content-Type: text/plain; charset="us-ascii" > > I still use that. It is 70% with 5mL of hydrochloric acid if making 1L. > Lynn Burton > Lab Assoc I > Animal Disease Lab > Galesburg, Il > 309-344-2451 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [sdysart@mirnarx.com] > Sent: Thursday, January 05, 2012 9:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amazing > > I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. ?That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). ?I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. ?I want to say it was like a 1% acid solution in alcohol?? ?What was the acid? ?For some reason my brain says glacial acetic...but time has made me forget. ?Is the alcohol you mix it in 100% or something lower with a water content to it? ?Please help my alzheimers =) > Happy Thursday!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas ?78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 7 > Date: Thu, 5 Jan 2012 17:16:43 +0000 > From: Sarah Dysart > Subject: [Histonet] Look at me... > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<8A70A9B2ECDD084DACFE6C59FCF86D509C9831@SN2PRD0702MB098.namprd07.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > I'm just full of questions today!! ?This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. ?I do all the blocking including Fc receptors...still junk. ?The clone I have been using is, from abcam (ab2302). ?I don't see the specific clone name listed. ?I am staining human xenografts raised in mouse. ?I get a whole lot of background staining making it very hard to find the positive staining. ?The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. ?At the higher dilution no positive staining or background is observed. ?Does anyone know of a good Caspase3 antibody, ?preferably mouse monoclonal? ?All the rabbit polyclonal antibodies are difficult to stain on these xenografts. > Thanks again =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas ?78744 > (512)901-0900 ext. 6912 > > > > ------------------------------ > > Message: 8 > Date: Thu, 5 Jan 2012 10:35:20 -0700 > From: "gayle callis" > Subject: [Histonet] Nail Polish sealant > To: , ? ? ?"Histonet" > ? ? ? ? > Message-ID: <000e01cccbd0$67966f50$36c34df0$@bresnan.net> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > You wrote: > > We are currently starting up some IHC on frozen tissue sections. ?After > staining with different fluorescent antibodies, we end with applying DAPI > w/Prolong gold and then coverslipping. ?We would like to seal the coverslip > so that we can keep the slides longer. ?Any suggestions on where and how > best to apply the nail polish for a permanent fix on the coverslips? > > > > ***************************** > > Prolong Gold antifade reagent is a hard seal to begin with. We only seal > ends but not the sides of cover glass. ?Our coverslips go right to edge of > slides e.g. 25 X 30, 25 x 40, or 25 X 50. ? ?We don't like having nail > polish slop over the edges onto back of slides but one could seal all sides > of coverslip if careful. ? ? We buy the thinner top or base coat nail > polish, but in general, prefer to use thinned mounting media rather than > nail polish. ? There can be some issues here. ? ?If you are trying to view > GFP or RFP (red fluorescence protein) labeled cells or tissue components, > you should not seal the coverslip with nail polish since the alcohol in nail > polish leaches under the coverslip and causes GFP/RFP to fade. ?This fact > was published in Science. ? We found that dumping out cheap clear nail > polish from bottle, rinsing away the residue with acetone, and then adding > permanent mounting media and thinning that with toluene to the consistency > of top coat nail polish works best. ?Toluene or xylene based sealants cannot > leach under the cover glass since these solvents are NOT miscible with water > in the PBS. ? ?Thinned mounting media is better sealant for GFP purposes (no > fading) and also works for IF stained sections (perfect seal). ?We love the > little brush in the nail polish bottle for application. ?Thicker clear nail > polish (for non GFP studies) or IF stained sections is messy during > application so we buy the cheapest top coat polish we can find at Walmart. > > > > > DAPI in the Prolong Gold will cause an uneven staining gradient so that some > of the nuclei in the center of a section are not stained as brightly as the > nuclei on the outer edges of a stained section. ?The cause is not getting > enough thicker Prolong Gold/DAPI over the section or not having just the > right amount of buffer on the section to permit a good flow of this > wonderful mounting media over the section. ? ?We now complete all IF > staining then stain with a DAPI solution before cover slipping with Prolong > Gold. ? You can buy ready to use DAPI solutions from Pierce or Biogenex, or > make up the solution in house. ? You can find the recipe at IHCworld website > or simply Google. > > > > We do NOT store our IF stained slides in the cold, but in a folder at RT in > a dark drawer before viewing on the day after staining to reduce any > movement/flow under the coverslip. ? Fluorophores can and will eventually > fade. ? I do not recall any studies saying storing IF stained slides in the > cold reduces fading but we never have space to do cold storage anyway and > store slides at RT. ? ?The new fluorophores (Alexas and Dylights) remain > stable over a longer time even for several weeks compared to fluorescein > derivatives e.g. FITC TRITC. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > > > ------------------------------ > > Message: 9 > Date: Thu, 5 Jan 2012 10:36:16 -0700 > From: Elizabeth Chlipala > Subject: [Histonet] RE: Look at me... > To: 'Sarah Dysart' , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<14E2C6176416974295479C64A11CB9AE011380AC7447@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > If you are looking to stain cleaved caspase 3 there is a better antibody from cell signaling it works in multiple species, it's a bit pricey but I have found that it works the best out of the few that I have tried. ?We have used it mouse xenografts before without any issue. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949?office > (303) 682-9060?fax > (303) 881-0763?cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart > Sent: Thursday, January 05, 2012 10:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Look at me... > > I'm just full of questions today!! ?This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. ?I do all the blocking including Fc receptors...still junk. ?The clone I have been using is, from abcam (ab2302). ?I don't see the specific clone name listed. ?I am staining human xenografts raised in mouse. ?I get a whole lot of background staining making it very hard to find the positive staining. ?The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. ?At the higher dilution no positive staining or background is observed. ?Does anyone know of a good Caspase3 antibody, ?preferably mouse monoclonal? ?All the rabbit polyclonal antibodies are difficult to stain on these xenografts. > Thanks again =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas ?78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Thu, 5 Jan 2012 09:45:25 -0800 > From: "Morken, Timothy" > Subject: [Histonet] Elastichrome paper, and troubleshooting > To: Histonet > Message-ID: > ? ? ? ?<8D7C2D242DBD45498006B21122072BF89F5EE62C@MCINFRWEM003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain: > > Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975 > > We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past. > > I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible. > > Our problem is that the elastic stain washes out during or after the trichrome. > > Procedure: > Bouins, 56C one hour > Wiegerts hematolylin, 3min > Verhoffs elastic stain, 15 min > 2% ferric chloride differentiation (so far so-good) > Gomori's trichrome Blue, 15 min > 0.2% glacial acetic acid, 1 min > Wash dH20, dehydrate, clear, mount > > Results: ?trichrome looks great, no elastin stain. > > I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions. > > Thanks for any help. > > > Tim Morken > Supervisor, Histology, IPOX > UC San Francisco Medical Center > Box 1656 > 1600 Divisidero St, B217 > San Francisco, CA 94115 > USA > > 415.514.6042?(office) > 415.885.7409?Fax > tim.morken@ucsfmedctr.org > > > > ------------------------------ > > Message: 11 > Date: Thu, 5 Jan 2012 10:50:05 -0700 > From: Elizabeth Chlipala > Subject: [Histonet] RE: Elastichrome paper, and troubleshooting > To: "'Morken, Timothy'" , Histonet > ? ? ? ? > Message-ID: > ? ? ? ?<14E2C6176416974295479C64A11CB9AE011380AC7449@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Tim > > We do this stain all of the time, we never used the original reference we just made up one on our own. ?I'll send our SOP in a different e-mail. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949?office > (303) 682-9060?fax > (303) 881-0763?cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, January 05, 2012 10:45 AM > To: Histonet > Subject: [Histonet] Elastichrome paper, and troubleshooting > > Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain: > > Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975 > > We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past. > > I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible. > > Our problem is that the elastic stain washes out during or after the trichrome. > > Procedure: > Bouins, 56C one hour > Wiegerts hematolylin, 3min > Verhoffs elastic stain, 15 min > 2% ferric chloride differentiation (so far so-good) > Gomori's trichrome Blue, 15 min > 0.2% glacial acetic acid, 1 min > Wash dH20, dehydrate, clear, mount > > Results: ?trichrome looks great, no elastin stain. > > I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions. > > Thanks for any help. > > > Tim Morken > Supervisor, Histology, IPOX > UC San Francisco Medical Center > Box 1656 > 1600 Divisidero St, B217 > San Francisco, CA 94115 > USA > > 415.514.6042?(office) > 415.885.7409?Fax > tim.morken@ucsfmedctr.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 6 > *************************************** From talulahgosh <@t> gmail.com Fri Jan 6 00:32:57 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 6 00:33:06 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D509C9964@SN2PRD0702MB098.namprd07.prod.outlook.com> References: <5A2BD13465E061429D6455C8D6B40E39137DA01FF8@IBMB7Exchange.digestivespecialists.com> <730597643.370044.1325795477561.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> <8A70A9B2ECDD084DACFE6C59FCF86D509C9964@SN2PRD0702MB098.namprd07.prod.outlook.com> Message-ID: When I work with xylene, it's always in the fume hood while wearing gloves. The same goes for formaldehyde. I never use a face shield for anything, I just do it in the fume hood if I need to use caution with a certain chemical. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From birems2 <@t> yahoo.com Fri Jan 6 04:01:27 2012 From: birems2 <@t> yahoo.com (Remedy Bi) Date: Fri Jan 6 04:01:32 2012 Subject: [Histonet] (no subject) Message-ID: <1325844087.40096.YahooMailClassic@web45504.mail.sp1.yahoo.com> Which is the best way to process small biopsies and lymph nodes manually? From tpodawiltz <@t> lrgh.org Fri Jan 6 05:05:33 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Jan 6 05:05:47 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: <730597643.370044.1325795477561.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <5A2BD13465E061429D6455C8D6B40E39137DA01FF8@IBMB7Exchange.digestivespecialists.com> <730597643.370044.1325795477561.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB5DD124@LRGHEXVS1.practice.lrgh.org> We have our fume hoods inspected twice per year and the air exchange rate in our room is 52 times per hour, so we do not wear respirators in the lab. We only wear the full face respirators in the hazardous waste storage are while we are transferring the waste into the storage containers. We do wear the xylene and formalin badges for testing once per year then have the results sent to employee health. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net Sent: Thursday, January 05, 2012 3:31 PM To: Linda Blazek Cc: histonet@lists.utsouthwestern.edu; Amy Self Subject: Re: [Histonet] Respirators and Routine Histology They must be in a liberal part of Michigan. ----- Original Message ----- From: "Linda Blazek" To: "Lisa Brenner" , "Amy Self" , "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 5, 2012 2:30:20 PM Subject: RE: [Histonet] Respirators and Routine Histology Nothing at all??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, January 05, 2012 3:22 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Respirators and Routine Histology No, not here. We don't wear anything at all. We have excellent air flow, and we wear the badges that test for exposure to xylene and formalin annually which I think is a CAP requirement. Our results have been better than acceptable every year. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org>>> Amy Self 1/5/2012 3:09 PM >>> Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From TMcNemar <@t> lmhealth.org Fri Jan 6 05:11:42 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jan 6 05:11:37 2012 Subject: [Histonet] RE: Respirators and Routine Histology In-Reply-To: References: Message-ID: We have respirators for formaldehyde only. They are available for use by anyone if they if they want to use one but they are only required in the case of a formalin spill. We do formalin monitoring at least yearly and sometimes in between. Employees are fit tested with our respirators on a yearly basis. Our exhaust is very good and our exposure for an 8 hour period is virtually non-detectable. We use a ventilated grossing station that is certified yearly. We have masks with shield available if someone wants to wear one (and we do occasionally but nothing is required for the full shift. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Thursday, January 05, 2012 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Respirators and Routine Histology Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rsrichmond <@t> gmail.com Fri Jan 6 05:26:57 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Jan 6 05:27:00 2012 Subject: [Histonet] Re: saffron vs. safran du gatinais Message-ID: Saffron is of course the dried stigmas of Crocus sativus. Safran du G?tinais, commonly specified in old histology books, was apparently grown in a particular area of France near the Mediterranean. Saffron is so expensive to grow that it isn't much cultivated in Europe. Most commercial saffron is grown in Pakistan or Kashmir, I think. There is no synthetic substitute. You can get excellent quality culinary saffron from Penzey's spices, though I don't know how it works as a dye - you could probably ask them. The Wikipedia article is very much worth reading. Apparently there is an ISO standard for saffron. For histologic use, saffron is extracted with hot ethanol. Some people did seven extractions. Some people used a reflux condenser for the hot alcohol, if you can find a geezer like me who knows what a reflux condenser is. The alcoholic extract smells terrible. The most common use is as the hematoxylin-phloxin-saffron (HPS) trichrome stain. It was in use as a general oversight stain in a few pathology services when I was a resident in the 1960's, most notably at Columbia-Presbyterian Hospital in New York City. Bob Richmond Samurai Pathologist Knoxville TN From b-frederick <@t> northwestern.edu Fri Jan 6 07:36:56 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jan 6 07:37:02 2012 Subject: [Histonet] saffron vs. safran du gatinais In-Reply-To: <000301cccbf8$51f9b8f0$f5ed2ad0$@bresnan.net> References: <000301cccbf8$51f9b8f0$f5ed2ad0$@bresnan.net> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E19DE0F@evcspmbx3.ads.northwestern.edu> We buy our alcoholic saffron from Rowley biochemical. Already made and reusable. We were still using it when it hit its expiration date. You can but all the Movat's reagents as a kit or but what you need. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, January 05, 2012 4:21 PM To: 'Villarreal, Beth'; Histonet Subject: RE: [Histonet] saffron vs. safran du gatinais Beth, In the past, people replied to Histonet with the suggestion to buy saffron aka safran du gatinais from a grocery store spice section. Depending on where you are located e.g. a bigger city, try to find a store that sells spices from India may have the freshest saffron. However, you won't suffer the sticker shock of buying it from a chemical supplier. It still tends to be expensive but not like chemical company prices. Once we made the alcoholic saffron solution for Movat's pentachrome, we stored this solution in a container with a dessicant to maintain a water free environment. I was fascinated that Tom actually grew and harvested saffron from the flowers. That is true devotion, but I suspect it was for those delicious sounding "nubbies . Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Villarreal, Beth Sent: Thursday, January 05, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri Jan 6 07:58:24 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jan 6 07:58:39 2012 Subject: [Histonet] Respirators and Routine Histology In-Reply-To: References: Message-ID: Respirators are required when you have a task that has proven to be above the recommend PPM trace gas analysis. I've only seen that once in umpteen years. Usually a hood is fine. If you have concerns you should always do another badge analysis. Wearing a respirator all day is not an option in my book. Happy weekend! Kim D Sent from my iPhone On Jan 5, 2012, at 3:09 PM, Amy Self wrote: > Happy New Year to All, > > I need some help from all of you out there in histoland. > > How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? > > Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. > > Thanks in advance for all of your help, Amy > > > Amy Self > Georgetown Hospital System > 843-527-7179 > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrew <@t> histologytechservices.com Fri Jan 6 08:13:38 2012 From: andrew <@t> histologytechservices.com (Andrew Brown) Date: Fri Jan 6 08:13:52 2012 Subject: [Histonet] Used Equipment Needed Message-ID: <88D256659EDE437CB188464C3906E8B0@AdminManager> Hello, Does anybody out there have any of the equipment below that they are looking to get rid of or sell? Any help with this search would be greatly appreciated Leica ASP300 tissue processor Leica SM2000 R sliding microtome Shandon paraffin dispenser Andrew Brown HT (ASCP) Florida Licensed HT Lab Technical Coordinator Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. From kgrobert <@t> rci.rutgers.edu Fri Jan 6 08:25:08 2012 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Jan 6 08:25:11 2012 Subject: [Histonet] Re: respirators and routine histology Message-ID: Under normal circumstances, I don't wear a respirator for routine histology, as our airflow is very good. I attempted it when I was pregnant with my daughter...but it didn't last long as the mask pressed on a certain spot under my chin and made me want to throw up all the time, even after the morning sickness had passed. After that, I passed fume-intensive tasks that could not be done under the hood (like changing the processor)to coworkers and stayed out of the room while they were being done. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From TNMayer <@t> mdanderson.org Fri Jan 6 08:33:54 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Jan 6 08:33:58 2012 Subject: [Histonet] RE:decal solution for molecular studies In-Reply-To: <6175d1b6-5f27-4d0d-927e-34de935bfc9a@DCPWPRTR02.mdanderson.edu> References: <6175d1b6-5f27-4d0d-927e-34de935bfc9a@DCPWPRTR02.mdanderson.edu> Message-ID: Clare, I just checked with our MB instructors and they said that the decal with EDTA should be ok, as long as you rinse it well after using, to remove the residual acid. Hope that helps. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 5 Jan 2012 10:16:06 -0500 > From: Clare Thornton > Subject: [Histonet] RE: decal solution for molecular studies > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > To clarify, the Formical we use is a formic acid/EDTA solution. > > > Clare J. Thornton, HTL(ASCP), QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton > Sent: Thursday, January 05, 2012 9:12 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] decal solution for molecular studies > > Does anyone know of a decal solution that enables molecular studies (Her 2 FISH, EGFR by PCR) to be performed later? ?We use Formical for our decal solution. ?Her 2 FISH works about 50% of the time, EGFR almost never. ?We are looking at having to cut undecalcified bone today for both these studies, and we are not equipped to cut undecalcified bone. ?Any suggestions? > > > > Clare J. Thornton, HTL(ASCP), QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************ From asmith <@t> mail.barry.edu Fri Jan 6 08:38:56 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Jan 6 08:39:27 2012 Subject: [Histonet] topoisomerase IHC Message-ID: I am having difficulty localizing topoisomerase IIalpha with IHC. Mouse primary/biotinylated horse secondary works for me only some of the time. Rabbit primary/biotinylated goat secondary doesn't work at all for me. Does anyone have any suggestions for improving the performance of either system? From NMP <@t> stowers.org Fri Jan 6 09:34:50 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Fri Jan 6 09:34:54 2012 Subject: [Histonet] Slide Brite Message-ID: <2C40E43D1F7A56408C4463FD245DDDF995135671@EXCHMB-02.stowers-institute.org> Hello. I saw a post this morning about Slide Brite substitute for Xylene and would like to get your opinion(s) on the product. Thank you, Nanne Marsh HT (ASCP) Histology Specialist II 1000 E 50th Street Kansas City, MO. 64110 (816) 926-4305 nmp@stowers.org From shehnazster <@t> gmail.com Fri Jan 6 09:37:37 2012 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Fri Jan 6 09:37:41 2012 Subject: [Histonet] Tests not FDA -approved / cleared Message-ID: Hi netters, Wondering what's been done for the following CAP standard (COM 40200): Tests not FDA -approved / cleared. "The lab has a list of tests not approved /cleared by FDA that have been implemented during the previous two years". Does this apply to special stains too? Is this applicable for IHC done manually using Dako antibodies? Thanks in advance. S Kahn Chief scientist From nicole <@t> dlcjax.com Fri Jan 6 10:50:10 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Fri Jan 6 10:02:31 2012 Subject: [Histonet] Slide Brite In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF995135671@EXCHMB-02.stowers-institute. org> References: <2C40E43D1F7A56408C4463FD245DDDF995135671@EXCHMB-02.stowers-institute.org> Message-ID: <2211.208.62.167.196.1325868610.squirrel@webmail.realpages.com> I have used this product for about 10yrs and love it. It has no fumes and no foul odor like the orange smelly stuff which gives me the worst headache. I use it in my processor. To deparrafinize and in my routine stain line. It works great with special stains to. I have not used it for IHC since the lab I work in does not deal with that test. It is non toxic as well. I purchase my supply from Chad at Mercedes Medical. P.S. There are few draw backs. I feel that the solution has to be changed more often than xylene as it becomes saturated faster. It also does not remove coverslip as well as xylene. Nicole Tatum, HT ASCP Hello. I saw a post this morning about Slide Brite substitute for Xylene > and would like to get your opinion(s) on the product. Thank you, > > Nanne Marsh HT (ASCP) > > Histology Specialist II > 1000 E 50th Street > Kansas City, MO. 64110 > (816) 926-4305 > nmp@stowers.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJohnson <@t> gnf.org Fri Jan 6 10:13:10 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Fri Jan 6 10:13:14 2012 Subject: [Histonet] A nit to pick - background IHC staining Message-ID: <9F3CFEE76E51B64991C7485270890B4009EE2828@EX4.lj.gnf.org> Happy Friday to you all! I just wanted to comment on the idea that when detecting mouse antibodies on mouse tissues gives you background staining. I consider background staining to be non-specific binding of some reagent to the tissue that is then detected with the chromogen or fluorophore. Anti-mouse antibodies specifically bind to the mouse Igs in the tissue as well as to the mouse Ig labeled antigen from the antibody. It's a nuisance and not specific to your target, but I don't consider it background. As previously mentioned, mouse on mouse kits work well to minimize this. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 6 10:48:55 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jan 6 10:49:12 2012 Subject: [Histonet] Tests not FDA -approved / cleared In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE64C@MCINFRWEM003.ucsfmedicalcenter.org> S Khan, I don't think it applies to special stains. I've never seen anything that said Special Stains are regulated by FDA (more correctly, the companies that sell special stains do not require FDA regulation). I guess it is because they are historically exempt or something. I do notice that some companies, like DAKO label their special stain kits as "IVD" but other companies do not. But this mainly applies to antibodies and ISH probes, so it's an interesting question for the histo lab because the vast majority of antibodies used by labs are Class I exempt IVD's or ASR's that are not "approved/cleared by FDA." (you have to note that on your reports, but CAP has also recommended putting a second disclaimer that those antibodies do not require approval). That is because they are officially exempt from regulation because they are considered ancillary tests that do not require FDA regulation. Vendors do not supply any test information to FDA about Class I antibodies. The IVD status simply denotes they were produced by an FDA-certified vendor and so the quality should be satisfactory. So, what is the point of listing all those antibodies? I don't know. On the other hand, some labs use Research Use Only (RUO) antibodies that they validate themselves. CLIA allows that though FDA and CAP discourage the practice (CLIA regulates labs, FDA regulates vendors of reagents). I think these are the ones CAP is really interested in. Maybe gathering data?? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shehnaz khan Sent: Friday, January 06, 2012 7:38 AM To: histonet@lists.utsouthwestern.edu Cc: histonet@pathology.swmed.edu Subject: [Histonet] Tests not FDA -approved / cleared Hi netters, Wondering what's been done for the following CAP standard (COM 40200): Tests not FDA -approved / cleared. "The lab has a list of tests not approved /cleared by FDA that have been implemented during the previous two years". Does this apply to special stains too? Is this applicable for IHC done manually using Dako antibodies? Thanks in advance. S Kahn Chief scientist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From langleyk <@t> mercyhealth.com Fri Jan 6 11:05:39 2012 From: langleyk <@t> mercyhealth.com (Kristi Langley) Date: Fri Jan 6 11:06:40 2012 Subject: [Histonet] Cassette labelers Message-ID: <4F06D58302000039000065AA@nodcdmg2.no.trinity-health.org> Hello all! TGIF....We are currently looking at purchasing a Leica IPC cassette labeler. We want to interface with our Cerner Millennium. Can anyone that currently uses this labeler let me know what they think? Likes, dislikes or if you are using another brand of labeler, your feedback on that as well please:) Many thanks Kristi Langley HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 801 5th St. Sioux City, Iowa 51101 712-279-2768 From Lynn.Burton <@t> Illinois.gov Fri Jan 6 11:43:47 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Jan 6 11:45:37 2012 Subject: [Histonet] Slide Brite In-Reply-To: <2211.208.62.167.196.1325868610.squirrel@webmail.realpages.com> References: <2C40E43D1F7A56408C4463FD245DDDF995135671@EXCHMB-02.stowers-institute.org>, <2211.208.62.167.196.1325868610.squirrel@webmail.realpages.com> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC006483C0725@IL084EXMBX214.illinois.gov> We have used this product for many years as well. It has performed well without the hazards of xylene. We still use xylene on the processor for cleaning. I have not used it in special stains, only on the processor. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum [nicole@dlcjax.com] Sent: Friday, January 06, 2012 10:50 AM To: Marsh, Nannette; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Slide Brite I have used this product for about 10yrs and love it. It has no fumes and no foul odor like the orange smelly stuff which gives me the worst headache. I use it in my processor. To deparrafinize and in my routine stain line. It works great with special stains to. I have not used it for IHC since the lab I work in does not deal with that test. It is non toxic as well. I purchase my supply from Chad at Mercedes Medical. P.S. There are few draw backs. I feel that the solution has to be changed more often than xylene as it becomes saturated faster. It also does not remove coverslip as well as xylene. Nicole Tatum, HT ASCP Hello. I saw a post this morning about Slide Brite substitute for Xylene > and would like to get your opinion(s) on the product. Thank you, > > Nanne Marsh HT (ASCP) > > Histology Specialist II > 1000 E 50th Street > Kansas City, MO. 64110 > (816) 926-4305 > nmp@stowers.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Fri Jan 6 12:06:40 2012 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Fri Jan 6 12:06:46 2012 Subject: [Histonet] finger nails Message-ID: <1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services From tkngflght <@t> yahoo.com Fri Jan 6 12:51:12 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jan 6 12:51:16 2012 Subject: [Histonet] Temporary & Direct hire PA(ASCP) for February/March (Refer a Friend) Message-ID: <1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> Hello 'Netters- ? Do you know any temp/travel PAs?? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!?? If you refer someone we help, you get a referral bonus (keep it or share it with your friend!) ? 1. Temp traveler for at least 4 weeks in mid-February. 2. Direct Hire in 6 different institutions around the US.? Entry through Supervisory.? ? As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide. ? Give me a call--make sure those you refer mention your name--I LIKE writing referral checks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From gagnone <@t> KGH.KARI.NET Fri Jan 6 14:19:18 2012 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Jan 6 14:19:32 2012 Subject: [Histonet] Saffron Message-ID: Beth, as Bob Richmond has noted regarding saffron, "The most common use is as the hematoxylin-phloxin-saffron (HPS) trichrome stain. It was in use as a general oversight stain in a few pathology services when I was a resident in the 1960's..." and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain. We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change. I still think the wafting of the boiling saffron is quite a pleasant aroma. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From rjbuesa <@t> yahoo.com Fri Jan 6 14:45:08 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 6 14:45:12 2012 Subject: [Histonet] finger nails In-Reply-To: <1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> Message-ID: <1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> See attachment! Ren? J. --- On Fri, 1/6/12, Michele Carr wrote: From: Michele Carr Subject: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" Date: Friday, January 6, 2012, 1:06 PM Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Jan 6 16:06:19 2012 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jan 6 16:06:27 2012 Subject: [Histonet] Quality assurance program for pathologists Message-ID: Is anyone willing to share with me their quality assurance/management program for pathologists. Sincere thanks Diana From rsrichmond <@t> gmail.com Sat Jan 7 12:40:26 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 7 12:40:31 2012 Subject: [Histonet] Re: Quality assurance program for pathologists Message-ID: Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN From we3smitty <@t> yahoo.com Sat Jan 7 17:00:29 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Sat Jan 7 17:00:33 2012 Subject: [Histonet] finger nails In-Reply-To: <1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> Message-ID: <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. --- On Fri, 1/6/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" Date: Friday, January 6, 2012, 3:45 PM See attachment! Ren? J. --- On Fri, 1/6/12, Michele Carr wrote: From: Michele Carr Subject: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" Date: Friday, January 6, 2012, 1:06 PM Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Sat Jan 7 18:40:39 2012 From: michelecarr10 <@t> yahoo.com (Michele Email) Date: Sat Jan 7 18:40:45 2012 Subject: [Histonet] finger nails In-Reply-To: <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> References: <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> Message-ID: Thanks everyone for the tips will try the Nair next time around. Michele Carr Sent from my iPad On Jan 7, 2012, at 3:00 PM, angela smith wrote: > Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Sat Jan 7 22:04:29 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Jan 7 22:04:33 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 In-Reply-To: <4f088854.29afec0a.6db5.6fa1SMTPIN_ADDED@mx.google.com> References: <4f088854.29afec0a.6db5.6fa1SMTPIN_ADDED@mx.google.com> Message-ID: Michele, This is our procedure for toe & finger nails; 1) Pre-fix nails in 10% NFB as usual 2) Soak fixed nails in NAIR or any counter nail remover until soften or bend easily (toe nail take longer; thickness dependent) 3) wash nail with water 4) Process in tissue processor 5) embed & cut Note; we found soften with NAIR before processing work the best. If NAIR is used after processing; 1) cut nail on charge slides (+) 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly 3) bake jar in ~ 60c over for ~ 30 min 4) cool & open jar in fume hood ~ 5 min 5) stain as usual Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Jan 7, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. finger nails (Michele Carr) > ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a > ? ? ?Friend) (Cheryl) > ? 3. Saffron (Gagnon, Eric) > ? 4. Re: finger nails (Rene J Buesa) > ? 5. Quality assurance program for pathologists (Diana McCaig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > > ------------------------------ > > Message: 2 > Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) > From: Cheryl > Subject: [Histonet] Temporary & Direct hire PA(ASCP) for > ? ? ? ?February/March ?(Refer a Friend) > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello 'Netters- > > Do you know any temp/travel PAs?? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!?? If you refer someone we help, you get a referral bonus (keep it or share it with your friend!) > > 1. Temp traveler for at least 4 weeks in mid-February. > 2. Direct Hire in 6 different institutions around the US.? Entry through Supervisory. > > As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide. > > Give me a call--make sure those you refer mention your name--I LIKE writing referral checks! > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT?Tech at a time. > 281.852.9457?Office > 800.756.3309?Phone & Fax > admin@fullstaff.org > > Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. > > ------------------------------ > > Message: 3 > Date: Fri, 6 Jan 2012 15:19:18 -0500 > From: "Gagnon, Eric" > Subject: [Histonet] Saffron > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > Beth, as Bob Richmond has noted regarding saffron, > > "The most common use is as the hematoxylin-phloxin-saffron (HPS) > trichrome stain. It was in use as a general oversight stain in a few > pathology services when I was a resident in the 1960's..." > > and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain. > > We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. ?Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. ?One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. ?Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change. > > I still think the wafting of the boiling saffron is quite a pleasant aroma. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Michele Carr > ? ? ? ? > Message-ID: > ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 6 Jan 2012 17:06:19 -0500 > From: "Diana McCaig" > Subject: [Histonet] Quality assurance program for pathologists > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Is anyone willing to share with me their quality assurance/management > program for pathologists. > > > > Sincere thanks > > Diana > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 9 > *************************************** From gladys.science <@t> gmail.com Sun Jan 8 10:18:08 2012 From: gladys.science <@t> gmail.com (Gladys Lim) Date: Sun Jan 8 10:18:11 2012 Subject: [Histonet] Advice needed the different types of cytomorphologic stains Message-ID: Dear all, I am relatively new to this area of staining approach and therefore, I need some advise on the different types of cytomorphologic stains that are available. (1) Is it necessary to air-dry your sample prior to staining with any Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? (2) Has anyone tried using the Romanowski stains on sample that were not air-dried? What was the outcome of the staining? (3) Were there any distinct difference in terms of staining among the different types of white blood cells vs. malignant cancer cells? (4) Wouldn't air-drying of sample prior to Romanowski stain change the morphology of cells? (5) Any recommendation for other types of stains if I want to differentiate white blood cells from cancer cells, without any air-drying steps involved? Any advice is greatly appreciated. Thanks. Regards, Gladys From naveedafahim <@t> yahoo.ca Sun Jan 8 15:16:58 2012 From: naveedafahim <@t> yahoo.ca (naveeda arshad) Date: Sun Jan 8 15:17:03 2012 Subject: [Histonet] Re:ihc course Message-ID: <1326057418.98464.YahooMailClassic@web161706.mail.bf1.yahoo.com> does any one know?about?institute offering IHC coursethanks --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 10 To: histonet@lists.utsouthwestern.edu Received: Sunday, January 8, 2012, 4:16 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Quality assurance program for pathologists (Bob Richmond) ???2. Re: finger nails (angela smith) ???3. Re: finger nails (Michele Email) ???4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey) ???5. Advice needed the different types of cytomorphologic??? stains ? ? ? (Gladys Lim) ---------------------------------------------------------------------- Message: 1 Date: Sat, 7 Jan 2012 13:40:26 -0500 From: Bob Richmond Subject: [Histonet] Re: Quality assurance program for pathologists To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST) From: angela smith Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" ??? ,??? Michele Carr ??? , Rene J Buesa Message-ID: ??? <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. --- On Fri, 1/6/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" Date: Friday, January 6, 2012, 3:45 PM See attachment! Ren? J. --- On Fri, 1/6/12, Michele Carr wrote: From: Michele Carr Subject: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" Date: Friday, January 6, 2012, 1:06 PM Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 7 Jan 2012 16:40:39 -0800 From: Michele Email Subject: Re: [Histonet] finger nails To: angela smith Cc: "Histonet@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain;??? charset=utf-8 Thanks everyone for the tips will try the Nair next time around. Michele Carr Sent from my iPad On Jan 7, 2012, at 3:00 PM, angela smith wrote: > Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren?? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 7 Jan 2012 20:04:29 -0800 From: Madeleine Huey Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Michele, This is our procedure for toe & finger nails; 1) Pre-fix nails in 10% NFB as usual 2) Soak fixed nails in NAIR or any counter nail remover until soften or bend easily (toe nail take longer; thickness dependent) 3) wash nail with water 4) Process in tissue processor 5) embed & cut Note; we found soften with NAIR before processing work the best. If NAIR is used after processing; 1) cut nail on charge slides (+) 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly 3) bake jar in ~ 60c over for ~ 30 min 4) cool & open jar in fume hood ~ 5 min 5) stain as usual Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Jan 7, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. finger nails (Michele Carr) > ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a > ? ? ?Friend) (Cheryl) > ? 3. Saffron (Gagnon, Eric) > ? 4. Re: finger nails (Rene J Buesa) > ? 5. Quality assurance program for pathologists (Diana McCaig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > > ------------------------------ > > Message: 2 > Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) > From: Cheryl > Subject: [Histonet] Temporary & Direct hire PA(ASCP) for > ? ? ? ?February/March ?(Refer a Friend) > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello 'Netters- > > Do you know any temp/travel PAs?? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!?? If you refer someone we help, you get a referral bonus (keep it or share it with your friend!) > > 1. Temp traveler for at least 4 weeks in mid-February. > 2. Direct Hire in 6 different institutions around the US.? Entry through Supervisory. > > As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide. > > Give me a call--make sure those you refer mention your name--I LIKE writing referral checks! > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT?Tech at a time. > 281.852.9457?Office > 800.756.3309?Phone & Fax > admin@fullstaff.org > > Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. > > ------------------------------ > > Message: 3 > Date: Fri, 6 Jan 2012 15:19:18 -0500 > From: "Gagnon, Eric" > Subject: [Histonet] Saffron > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > Beth, as Bob Richmond has noted regarding saffron, > > "The most common use is as the hematoxylin-phloxin-saffron (HPS) > trichrome stain. It was in use as a general oversight stain in a few > pathology services when I was a resident in the 1960's..." > > and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain. > > We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. ?Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. ?One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. ?Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change. > > I still think the wafting of the boiling saffron is quite a pleasant aroma. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Michele Carr > ? ? ? ? > Message-ID: > ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 6 Jan 2012 17:06:19 -0500 > From: "Diana McCaig" > Subject: [Histonet] Quality assurance program for pathologists > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Is anyone willing to share with me their quality assurance/management > program for pathologists. > > > > Sincere thanks > > Diana > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 9 > *************************************** ------------------------------ Message: 5 Date: Mon, 9 Jan 2012 00:18:08 +0800 From: Gladys Lim Subject: [Histonet] Advice needed the different types of ??? cytomorphologic??? stains To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Dear all, I am relatively new to this area of staining approach and therefore, I need some advise on the different types of cytomorphologic stains that are available. (1) Is it necessary to air-dry your sample prior to staining with any Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? (2) Has anyone tried using the Romanowski stains on sample that were not air-dried? What was the outcome of the staining? (3) Were there any distinct difference in terms of staining among the different types of white blood cells vs. malignant cancer cells? (4) Wouldn't air-drying of sample prior to Romanowski stain change the morphology of cells? (5) Any recommendation for other types of stains if I want to differentiate white blood cells from cancer cells, without any air-drying steps involved? Any advice is greatly appreciated. Thanks. Regards, Gladys ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 10 **************************************** From one_angel_secret <@t> yahoo.com Sun Jan 8 16:27:29 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sun Jan 8 16:27:32 2012 Subject: [Histonet] Re: Quality assurance program for pathologists In-Reply-To: References: Message-ID: <1326061649.24557.YahooMailNeo@web112301.mail.gq1.yahoo.com> Dr Richmond, ?????????????????????? You always please me with your replies. You are such a value to these forums. Let me, I'm sure for the thousant time tell you how much you are appreciated. ? ? You clearly understand "Quality" ? as most any Pathologist that Ive ever been subject to. ? As you know 10% review is the minumum ? 100%........... yep!~ Ive seen that.. what a marvel of respect did that get from little ole me ? Yes, excessive, we both agree ? I have to say though to Diana, that usually the Pathologist have thier own QA program that monitors thier work. Ive never seen anything different. Even the lone Path. ? Its always a good idea to communicate with them ? They may already have the answer to your questions? ? Kim D ? ? ________________________________ From: Bob Richmond To: histonet@lists.utsouthwestern.edu Sent: Saturday, January 7, 2012 1:40 PM Subject: [Histonet] Re: Quality assurance program for pathologists Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sun Jan 8 16:40:46 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sun Jan 8 16:40:52 2012 Subject: [Histonet] Re:ihc course In-Reply-To: <1326057418.98464.YahooMailClassic@web161706.mail.bf1.yahoo.com> References: <1326057418.98464.YahooMailClassic@web161706.mail.bf1.yahoo.com> Message-ID: <1326062446.12201.YahooMailNeo@web112306.mail.gq1.yahoo.com> You can get IHC education from most of the vendors. If you cant go that route, then there are many good books and subscriber publications out. ? If you really want to be on top of the subject, do what I did many many years ago. ? It's called do it yourself education ? Get one of the books they give you to order from and learn from it. It always tells you what each antibody stains for, whats the control etc ? You actually can go online to any of the major sites such as DAKO, LEICA or VENTANA and see thier antibody list with all the applied info for them. ? In America you can also obtain knowledge from the NSH( http://www.nsh.org/?) or as in Florida we have FSH (http://www.fshgroup.org/?) ? Much Luck ? Kim D ________________________________ From: naveeda arshad To: histonet@lists.utsouthwestern.edu Sent: Sunday, January 8, 2012 4:16 PM Subject: [Histonet] Re:ihc course does any one know?about?institute offering IHC coursethanks --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 10 To: histonet@lists.utsouthwestern.edu Received: Sunday, January 8, 2012, 4:16 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Quality assurance program for pathologists (Bob Richmond) ???2. Re: finger nails (angela smith) ???3. Re: finger nails (Michele Email) ???4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey) ???5. Advice needed the different types of cytomorphologic??? stains ? ? ? (Gladys Lim) ---------------------------------------------------------------------- Message: 1 Date: Sat, 7 Jan 2012 13:40:26 -0500 From: Bob Richmond Subject: [Histonet] Re: Quality assurance program for pathologists To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST) From: angela smith Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" ??? ,??? Michele Carr ??? , Rene J Buesa Message-ID: ??? <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. --- On Fri, 1/6/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" Date: Friday, January 6, 2012, 3:45 PM See attachment! Ren? J. --- On Fri, 1/6/12, Michele Carr wrote: From: Michele Carr Subject: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" Date: Friday, January 6, 2012, 1:06 PM Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 7 Jan 2012 16:40:39 -0800 From: Michele Email Subject: Re: [Histonet] finger nails To: angela smith Cc: "Histonet@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain;??? charset=utf-8 Thanks everyone for the tips will try the Nair next time around. Michele Carr Sent from my iPad On Jan 7, 2012, at 3:00 PM, angela smith wrote: > Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren?? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 7 Jan 2012 20:04:29 -0800 From: Madeleine Huey Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Michele, This is our procedure for toe & finger nails; 1) Pre-fix nails in 10% NFB as usual 2) Soak fixed nails in NAIR or any counter nail remover until soften or bend easily (toe nail take longer; thickness dependent) 3) wash nail with water 4) Process in tissue processor 5) embed & cut Note; we found soften with NAIR before processing work the best. If NAIR is used after processing; 1) cut nail on charge slides (+) 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly 3) bake jar in ~ 60c over for ~ 30 min 4) cool & open jar in fume hood ~ 5 min 5) stain as usual Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Jan 7, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. finger nails (Michele Carr) > ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a > ? ? ?Friend) (Cheryl) > ? 3. Saffron (Gagnon, Eric) > ? 4. Re: finger nails (Rene J Buesa) > ? 5. Quality assurance program for pathologists (Diana McCaig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > > ------------------------------ > > Message: 2 > Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) > From: Cheryl > Subject: [Histonet] Temporary & Direct hire PA(ASCP) for > ? ? ? ?February/March ?(Refer a Friend) > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello 'Netters- > > Do you know any temp/travel PAs?? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!?? If you refer someone we help, you get a referral bonus (keep it or share it with your friend!) > > 1. Temp traveler for at least 4 weeks in mid-February. > 2. Direct Hire in 6 different institutions around the US.? Entry through Supervisory. > > As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide. > > Give me a call--make sure those you refer mention your name--I LIKE writing referral checks! > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT?Tech at a time. > 281.852.9457?Office > 800.756.3309?Phone & Fax > admin@fullstaff.org > > Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. > > ------------------------------ > > Message: 3 > Date: Fri, 6 Jan 2012 15:19:18 -0500 > From: "Gagnon, Eric" > Subject: [Histonet] Saffron > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > Beth, as Bob Richmond has noted regarding saffron, > > "The most common use is as the hematoxylin-phloxin-saffron (HPS) > trichrome stain. It was in use as a general oversight stain in a few > pathology services when I was a resident in the 1960's..." > > and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain. > > We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. ?Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. ?One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. ?Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change. > > I still think the wafting of the boiling saffron is quite a pleasant aroma. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Michele Carr > ? ? ? ? > Message-ID: > ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 6 Jan 2012 17:06:19 -0500 > From: "Diana McCaig" > Subject: [Histonet] Quality assurance program for pathologists > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Is anyone willing to share with me their quality assurance/management > program for pathologists. > > > > Sincere thanks > > Diana > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 9 > *************************************** ------------------------------ Message: 5 Date: Mon, 9 Jan 2012 00:18:08 +0800 From: Gladys Lim Subject: [Histonet] Advice needed the different types of ??? cytomorphologic??? stains To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Dear all, I am relatively new to this area of staining approach and therefore, I need some advise on the different types of cytomorphologic stains that are available. (1) Is it necessary to air-dry your sample prior to staining with any Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? (2) Has anyone tried using the Romanowski stains on sample that were not air-dried? What was the outcome of the staining? (3) Were there any distinct difference in terms of staining among the different types of white blood cells vs. malignant cancer cells? (4) Wouldn't air-drying of sample prior to Romanowski stain change the morphology of cells? (5) Any recommendation for other types of stains if I want to differentiate white blood cells from cancer cells, without any air-drying steps involved? Any advice is greatly appreciated. Thanks. Regards, Gladys ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 10 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Jan 8 22:32:05 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Jan 8 22:32:07 2012 Subject: [Histonet] Re:ihc course In-Reply-To: <1326062446.12201.YahooMailNeo@web112306.mail.gq1.yahoo.com> References: <1326057418.98464.YahooMailClassic@web161706.mail.bf1.yahoo.com> <1326062446.12201.YahooMailNeo@web112306.mail.gq1.yahoo.com> Message-ID: NSH has a lot of IHC teleconferences: http://s3.goeshow.com/nsh/NSHTC2012/ereg949997.cfm?pg=home Under Register, you can download the brochure. NSH also has some IHC forums: July 13-14, 2012 in Hartford, CT. Not much information available yet, but keep checking. http://www.nsh.org/content/immunohistochemistry-forum Dako has a lot of downloadable booklets http://www.dako.com/us/index/knowledgecenter.htm But as for a college offering courses/degree in IHC - I don't know of any. There are courses on Immunology in general, and there are courses for the med tech training programs, specific to their immunology. For the histotech programs that are college/university based, the IHC is built into the curriculum, usually part of the special stains class, and you have to be accepted into the histotech program. So most histotechs learn it on-the-job, and attending their state and national symposiums. Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Kim Donadio Sent: Sunday, January 08, 2012 5:40 PM To: naveeda arshad ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re:ihc course You can get IHC education from most of the vendors. If you cant go that route, then there are many good books and subscriber publications out. If you really want to be on top of the subject, do what I did many many years ago. It's called do it yourself education Get one of the books they give you to order from and learn from it. It always tells you what each antibody stains for, whats the control etc You actually can go online to any of the major sites such as DAKO, LEICA or VENTANA and see thier antibody list with all the applied info for them. In America you can also obtain knowledge from the NSH( http://www.nsh.org/ ) or as in Florida we have FSH (http://www.fshgroup.org/ ) Much Luck Kim D ________________________________ From: naveeda arshad To: histonet@lists.utsouthwestern.edu Sent: Sunday, January 8, 2012 4:16 PM Subject: [Histonet] Re:ihc course does any one know about institute offering IHC coursethanks --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 10 To: histonet@lists.utsouthwestern.edu Received: Sunday, January 8, 2012, 4:16 PM From epchatfield <@t> ihis.org Mon Jan 9 07:25:01 2012 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Mon Jan 9 07:25:40 2012 Subject: [Histonet] Stability of Retic stain in Bone Marrow Message-ID: <4F0AB26D020000BC00014C4C@coregwia.peigov> Hi Everyone, I'm wondering if anyone has experienced issues with the stability of reticulin (Gordon and Sweets method) in bone marrow. We are seeing silver precipitant after ~1 week on our patient sections - our control is fine. Cheers, Elizabeth Peyton-Chatfield Histology Supervisor Queen Elizabeth Hospital Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From Sharon.Genest <@t> saskatoonhealthregion.ca Mon Jan 9 09:32:12 2012 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Mon Jan 9 09:32:32 2012 Subject: [Histonet] Respirators and Routine Histology Message-ID: Amy We use respirators when changing stainers and processors. Our Gross Technologists work in chemical hoods, our special stains and coverslipping are performed in chemical hoods. We anually measure air quality and our hood air flow. Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca From Sharon.Genest <@t> saskatoonhealthregion.ca Mon Jan 9 09:36:10 2012 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Mon Jan 9 09:39:03 2012 Subject: [Histonet] Electron Microscopy Proficiency testing Message-ID: Does anyone subscribe to a program for proficiency testing for Electron Microscopy? Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca From cornettl <@t> hotmail.com Mon Jan 9 09:44:14 2012 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Mon Jan 9 09:44:19 2012 Subject: [Histonet] Controls Message-ID: We are in need of Aspergillus and Amyloid controls. Does anyone on the histonet have any suggestions, or overabundance that they could share with us? Thanks, Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 From njohnson <@t> kcskincenter.com Mon Jan 9 10:00:48 2012 From: njohnson <@t> kcskincenter.com (Nacaela Johnson) Date: Mon Jan 9 10:00:58 2012 Subject: [Histonet] Stability of Retic stain in Bone Marrow In-Reply-To: <4F0AB26D020000BC00014C4C@coregwia.peigov> References: <4F0AB26D020000BC00014C4C@coregwia.peigov> Message-ID: <000101cccee7$db9e1780$92da4680$@com> My experience with the Gordon and Sweets Reticulin is 1 to 2 days max. I found better results and stability with the Gomoris Retic from Poly Scientific. Nacaela Johnson, B.S. HTL (ASCP) Histology Supervisor Kansas City Skin & Cancer Center, LLC 5810 NW Barry Rd, Ste 100 Kansas City, MO 64154 ph: 816 584 8100 fx: 816 584 8106 em: njohnson@kcskincenter.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Monday, January 09, 2012 7:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stability of Retic stain in Bone Marrow Hi Everyone, I'm wondering if anyone has experienced issues with the stability of reticulin (Gordon and Sweets method) in bone marrow. We are seeing silver precipitant after ~1 week on our patient sections - our control is fine. Cheers, Elizabeth Peyton-Chatfield Histology Supervisor Queen Elizabeth Hospital Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judi <@t> medialabinc.net Mon Jan 9 10:55:34 2012 From: judi <@t> medialabinc.net (Judi Bennett) Date: Mon Jan 9 10:55:43 2012 Subject: [Histonet] Histology Authors Needed! Message-ID: *HISTOLOGY* authors needed ? especially for *MOLECULAR* courses! Actively seeking authors to write online *histology courses* for *MediaLab*! MediaLab is a leading publisher of online continuing education (CE) courses and competency assessments. Our online products are used at more than 2,000 laboratories and university CLS programs worldwide. This is a great opportunity to: - Gain* resume-boosting publishing experience* - *Earn honorariums* for your participation - Fill the *critical need for quality histology CE credits* Authors can take advantage of MediaLab's online CourseBuilder to *write courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive interface similar to Microsoft Word or PowerPoint. Authors can quickly create content pages, practice questions, and exam questions, and upload relevant images. Courses developed by MediaLab are *featured on our websites MediaLabInc.net and LabCE.com*. Questions from these courses also become part of the LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers may also *contribute to other online programs that we develop on behalf of major laboratory partners*. To learn more about becoming a MediaLab author for histology courses, visit our online information page at www.medialbinc.net/authors.aspx . Please contact Judi Bennett at judi@medialabinc.net or call 877-776-8460, ext. 721. Judi Bennett Program Director, MediaLab, Inc. -- Judi Bennett, BSM, MT MediaLab, Inc. e-mail judi@medialabinc.net Phone (877) 776-8460 ext. 721 cell phone 404-915-2999 fax (678) 401-0284 From shive003 <@t> umn.edu Mon Jan 9 11:25:52 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jan 9 11:26:00 2012 Subject: [Histonet] Electron Microscopy Proficiency testing In-Reply-To: References: Message-ID: We participate in the External Quality Assessment Scheme in EM Virus Diagnostics (EQA-EMV) administered through the Robert Koch Institut of Berlin, Germany. It tests proficiency only on infectious disease identification. Jan Shivers Section Head - EM University of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN, USA On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR < Sharon.Genest@saskatoonhealthregion.ca> wrote: > Does anyone subscribe to a program for proficiency testing for Electron > Microscopy? > > Sharon Genest > Anatomic Pathology > Process Improvement > Saskatoon Health Region > 306-655-8242 > sharon.genest@saskatoonhealthregion.ca > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TWELLEN <@t> LHS.ORG Mon Jan 9 12:10:51 2012 From: TWELLEN <@t> LHS.ORG (Wellen, Terrence D. :LPH Lab) Date: Mon Jan 9 12:11:02 2012 Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 11 In-Reply-To: <201201091802.q09I2KeU006311@email2.lhs.org> Message-ID: <5F46C87AD649864F9FA8273E8E31507A160729@SWM2006.LHSNT.LEGACYHS> ventana offers a basic course in tuscon. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, January 09, 2012 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re:ihc course (naveeda arshad) 2. Re: Re: Quality assurance program for pathologists (Kim Donadio) 3. Re: Re:ihc course (Kim Donadio) 4. Re: Re:ihc course (Lee & Peggy Wenk) 5. Stability of Retic stain in Bone Marrow (Elizabeth Chatfield) 6. Respirators and Routine Histology (Genest, Sharon SktnHR) 7. Electron Microscopy Proficiency testing (Genest, Sharon SktnHR) 8. Controls (Lorraine Cornett) 9. RE: Stability of Retic stain in Bone Marrow (Nacaela Johnson) 10. Histology Authors Needed! (Judi Bennett) 11. Re: Electron Microscopy Proficiency testing (Jan Shivers) ---------------------------------------------------------------------- Message: 1 Date: Sun, 8 Jan 2012 13:16:58 -0800 (PST) From: naveeda arshad Subject: [Histonet] Re:ihc course To: histonet@lists.utsouthwestern.edu Message-ID: <1326057418.98464.YahooMailClassic@web161706.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 does any one know?about?institute offering IHC coursethanks --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 10 To: histonet@lists.utsouthwestern.edu Received: Sunday, January 8, 2012, 4:16 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Quality assurance program for pathologists (Bob Richmond) ???2. Re: finger nails (angela smith) ???3. Re: finger nails (Michele Email) ???4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey) ???5. Advice needed the different types of cytomorphologic??? stains ? ? ? (Gladys Lim) ---------------------------------------------------------------------- Message: 1 Date: Sat, 7 Jan 2012 13:40:26 -0500 From: Bob Richmond Subject: [Histonet] Re: Quality assurance program for pathologists To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST) From: angela smith Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" ??? ,??? Michele Carr ??? , Rene J Buesa Message-ID: ??? <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. --- On Fri, 1/6/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" Date: Friday, January 6, 2012, 3:45 PM See attachment! Ren? J. --- On Fri, 1/6/12, Michele Carr wrote: From: Michele Carr Subject: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" Date: Friday, January 6, 2012, 1:06 PM Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 7 Jan 2012 16:40:39 -0800 From: Michele Email Subject: Re: [Histonet] finger nails To: angela smith Cc: "Histonet@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain;??? charset=utf-8 Thanks everyone for the tips will try the Nair next time around. Michele Carr Sent from my iPad On Jan 7, 2012, at 3:00 PM, angela smith wrote: > Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren?? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 7 Jan 2012 20:04:29 -0800 From: Madeleine Huey Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Michele, This is our procedure for toe & finger nails; 1) Pre-fix nails in 10% NFB as usual 2) Soak fixed nails in NAIR or any counter nail remover until soften or bend easily (toe nail take longer; thickness dependent) 3) wash nail with water 4) Process in tissue processor 5) embed & cut Note; we found soften with NAIR before processing work the best. If NAIR is used after processing; 1) cut nail on charge slides (+) 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly 3) bake jar in ~ 60c over for ~ 30 min 4) cool & open jar in fume hood ~ 5 min 5) stain as usual Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Jan 7, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. finger nails (Michele Carr) > ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a > ? ? ?Friend) (Cheryl) > ? 3. Saffron (Gagnon, Eric) > ? 4. Re: finger nails (Rene J Buesa) > ? 5. Quality assurance program for pathologists (Diana McCaig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > > ------------------------------ > > Message: 2 > Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) > From: Cheryl > Subject: [Histonet] Temporary & Direct hire PA(ASCP) for > ? ? ? ?February/March ?(Refer a Friend) > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello 'Netters- > > Do you know any temp/travel PAs?? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!?? If you refer someone we help, you get a referral bonus (keep it or share it with your friend!) > > 1. Temp traveler for at least 4 weeks in mid-February. > 2. Direct Hire in 6 different institutions around the US.? Entry through Supervisory. > > As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide. > > Give me a call--make sure those you refer mention your name--I LIKE writing referral checks! > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT?Tech at a time. > 281.852.9457?Office > 800.756.3309?Phone & Fax > admin@fullstaff.org > > Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. > > ------------------------------ > > Message: 3 > Date: Fri, 6 Jan 2012 15:19:18 -0500 > From: "Gagnon, Eric" > Subject: [Histonet] Saffron > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > Beth, as Bob Richmond has noted regarding saffron, > > "The most common use is as the hematoxylin-phloxin-saffron (HPS) > trichrome stain. It was in use as a general oversight stain in a few > pathology services when I was a resident in the 1960's..." > > and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain. > > We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. ?Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. ?One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. ?Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change. > > I still think the wafting of the boiling saffron is quite a pleasant aroma. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Michele Carr > ? ? ? ? > Message-ID: > ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 6 Jan 2012 17:06:19 -0500 > From: "Diana McCaig" > Subject: [Histonet] Quality assurance program for pathologists > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Is anyone willing to share with me their quality assurance/management > program for pathologists. > > > > Sincere thanks > > Diana > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 9 > *************************************** ------------------------------ Message: 5 Date: Mon, 9 Jan 2012 00:18:08 +0800 From: Gladys Lim Subject: [Histonet] Advice needed the different types of ??? cytomorphologic??? stains To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Dear all, I am relatively new to this area of staining approach and therefore, I need some advise on the different types of cytomorphologic stains that are available. (1) Is it necessary to air-dry your sample prior to staining with any Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? (2) Has anyone tried using the Romanowski stains on sample that were not air-dried? What was the outcome of the staining? (3) Were there any distinct difference in terms of staining among the different types of white blood cells vs. malignant cancer cells? (4) Wouldn't air-drying of sample prior to Romanowski stain change the morphology of cells? (5) Any recommendation for other types of stains if I want to differentiate white blood cells from cancer cells, without any air-drying steps involved? Any advice is greatly appreciated. Thanks. Regards, Gladys ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 10 **************************************** ------------------------------ Message: 2 Date: Sun, 8 Jan 2012 14:27:29 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Re: Quality assurance program for pathologists To: Bob Richmond , "histonet@lists.utsouthwestern.edu" Message-ID: <1326061649.24557.YahooMailNeo@web112301.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dr Richmond, ?????????????????????? You always please me with your replies. You are such a value to these forums. Let me, I'm sure for the thousant time tell you how much you are appreciated. ? ? You clearly understand "Quality" ? as most any Pathologist that Ive ever been subject to. ? As you know 10% review is the minumum ? 100%........... yep!~ Ive seen that.. what a marvel of respect did that get from little ole me ? Yes, excessive, we both agree ? I have to say though to Diana, that usually the Pathologist have thier own QA program that monitors thier work. Ive never seen anything different. Even the lone Path. ? Its always a good idea to communicate with them ? They may already have the answer to your questions? ? Kim D ? ? ________________________________ From: Bob Richmond To: histonet@lists.utsouthwestern.edu Sent: Saturday, January 7, 2012 1:40 PM Subject: [Histonet] Re: Quality assurance program for pathologists Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 8 Jan 2012 14:40:46 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Re:ihc course To: naveeda arshad , "histonet@lists.utsouthwestern.edu" Message-ID: <1326062446.12201.YahooMailNeo@web112306.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You can get IHC education from most of the vendors. If you cant go that route, then there are many good books and subscriber publications out. ? If you really want to be on top of the subject, do what I did many many years ago. ? It's called do it yourself education ? Get one of the books they give you to order from and learn from it. It always tells you what each antibody stains for, whats the control etc ? You actually can go online to any of the major sites such as DAKO, LEICA or VENTANA and see thier antibody list with all the applied info for them. ? In America you can also obtain knowledge from the NSH( http://www.nsh.org/?) or as in Florida we have FSH (http://www.fshgroup.org/?) ? Much Luck ? Kim D ________________________________ From: naveeda arshad To: histonet@lists.utsouthwestern.edu Sent: Sunday, January 8, 2012 4:16 PM Subject: [Histonet] Re:ihc course does any one know?about?institute offering IHC coursethanks --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 10 To: histonet@lists.utsouthwestern.edu Received: Sunday, January 8, 2012, 4:16 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Quality assurance program for pathologists (Bob Richmond) ???2. Re: finger nails (angela smith) ???3. Re: finger nails (Michele Email) ???4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey) ???5. Advice needed the different types of cytomorphologic??? stains ? ? ? (Gladys Lim) ---------------------------------------------------------------------- Message: 1 Date: Sat, 7 Jan 2012 13:40:26 -0500 From: Bob Richmond Subject: [Histonet] Re: Quality assurance program for pathologists To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Diana McCaig (where?) asks: "Is anyone willing to share with me their quality assurance/management program for pathologists?" Glad to know you're in charge of pathologists. (Also glad I'm nearly 73 years old, though not yet retired.) I've worked in a few programs that did a 10% review of cases. If you go this route, don't choose the cases at random, but ask the pathologists to designate the cases as they do the day's work - they'll catch a lot more problems that way. I've worked in a single practice that did 100% second-pathologist review (before the case was released), and I thought that was excessive. Pathologists should be encouraged to document their internal consultations - I mean when you pass a slide to the guy at the next microscope and ask him "from the ear of a 70 year old man - do you think there's enough here to call this a basal cell carcinoma?" Such cases should be documented in a comment - I say "Dr. John Doe has seen this material and concurs." Such cases are legitimately considered part of a 10% review policy. I've worked in one large and highly competent practice that documented internal consultation very meticulously, and one of their QA guidelines was that 2.8% of their cases document internal consultation. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST) From: angela smith Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" ??? ,??? Michele Carr ??? , Rene J Buesa Message-ID: ??? <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. --- On Fri, 1/6/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" Date: Friday, January 6, 2012, 3:45 PM See attachment! Ren? J. --- On Fri, 1/6/12, Michele Carr wrote: From: Michele Carr Subject: [Histonet] finger nails To: "Histonet@lists.utsouthwestern.edu" Date: Friday, January 6, 2012, 1:06 PM Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 7 Jan 2012 16:40:39 -0800 From: Michele Email Subject: Re: [Histonet] finger nails To: angela smith Cc: "Histonet@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain;??? charset=utf-8 Thanks everyone for the tips will try the Nair next time around. Michele Carr Sent from my iPad On Jan 7, 2012, at 3:00 PM, angela smith wrote: > Have you tried nairing it prior to fixation and processing?? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)? then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" , "Michele Carr" > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren?? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses.? > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 7 Jan 2012 20:04:29 -0800 From: Madeleine Huey Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Michele, This is our procedure for toe & finger nails; 1) Pre-fix nails in 10% NFB as usual 2) Soak fixed nails in NAIR or any counter nail remover until soften or bend easily (toe nail take longer; thickness dependent) 3) wash nail with water 4) Process in tissue processor 5) embed & cut Note; we found soften with NAIR before processing work the best. If NAIR is used after processing; 1) cut nail on charge slides (+) 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly 3) bake jar in ~ 60c over for ~ 30 min 4) cool & open jar in fume hood ~ 5 min 5) stain as usual Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Jan 7, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. finger nails (Michele Carr) > ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a > ? ? ?Friend) (Cheryl) > ? 3. Saffron (Gagnon, Eric) > ? 4. Re: finger nails (Rene J Buesa) > ? 5. Quality assurance program for pathologists (Diana McCaig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > > ------------------------------ > > Message: 2 > Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) > From: Cheryl > Subject: [Histonet] Temporary & Direct hire PA(ASCP) for > ? ? ? ?February/March ?(Refer a Friend) > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello 'Netters- > > Do you know any temp/travel PAs?? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!?? If you refer someone we help, you get a referral bonus (keep it or share it with your friend!) > > 1. Temp traveler for at least 4 weeks in mid-February. > 2. Direct Hire in 6 different institutions around the US.? Entry through Supervisory. > > As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide. > > Give me a call--make sure those you refer mention your name--I LIKE writing referral checks! > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT?Tech at a time. > 281.852.9457?Office > 800.756.3309?Phone & Fax > admin@fullstaff.org > > Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. > > ------------------------------ > > Message: 3 > Date: Fri, 6 Jan 2012 15:19:18 -0500 > From: "Gagnon, Eric" > Subject: [Histonet] Saffron > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > Beth, as Bob Richmond has noted regarding saffron, > > "The most common use is as the hematoxylin-phloxin-saffron (HPS) > trichrome stain. It was in use as a general oversight stain in a few > pathology services when I was a resident in the 1960's..." > > and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain. > > We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. ?Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. ?One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. ?Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change. > > I still think the wafting of the boiling saffron is quite a pleasant aroma. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Michele Carr > ? ? ? ? > Message-ID: > ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the slide during staining.? The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 6 Jan 2012 17:06:19 -0500 > From: "Diana McCaig" > Subject: [Histonet] Quality assurance program for pathologists > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Is anyone willing to share with me their quality assurance/management > program for pathologists. > > > > Sincere thanks > > Diana > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 9 > *************************************** ------------------------------ Message: 5 Date: Mon, 9 Jan 2012 00:18:08 +0800 From: Gladys Lim Subject: [Histonet] Advice needed the different types of ??? cytomorphologic??? stains To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Dear all, I am relatively new to this area of staining approach and therefore, I need some advise on the different types of cytomorphologic stains that are available. (1) Is it necessary to air-dry your sample prior to staining with any Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? (2) Has anyone tried using the Romanowski stains on sample that were not air-dried? What was the outcome of the staining? (3) Were there any distinct difference in terms of staining among the different types of white blood cells vs. malignant cancer cells? (4) Wouldn't air-drying of sample prior to Romanowski stain change the morphology of cells? (5) Any recommendation for other types of stains if I want to differentiate white blood cells from cancer cells, without any air-drying steps involved? Any advice is greatly appreciated. Thanks. Regards, Gladys ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 10 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 8 Jan 2012 23:32:05 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Re:ihc course To: "Kim Donadio" , "naveeda arshad" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original NSH has a lot of IHC teleconferences: http://s3.goeshow.com/nsh/NSHTC2012/ereg949997.cfm?pg=home Under Register, you can download the brochure. NSH also has some IHC forums: July 13-14, 2012 in Hartford, CT. Not much information available yet, but keep checking. http://www.nsh.org/content/immunohistochemistry-forum Dako has a lot of downloadable booklets http://www.dako.com/us/index/knowledgecenter.htm But as for a college offering courses/degree in IHC - I don't know of any. There are courses on Immunology in general, and there are courses for the med tech training programs, specific to their immunology. For the histotech programs that are college/university based, the IHC is built into the curriculum, usually part of the special stains class, and you have to be accepted into the histotech program. So most histotechs learn it on-the-job, and attending their state and national symposiums. Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Kim Donadio Sent: Sunday, January 08, 2012 5:40 PM To: naveeda arshad ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re:ihc course You can get IHC education from most of the vendors. If you cant go that route, then there are many good books and subscriber publications out. If you really want to be on top of the subject, do what I did many many years ago. It's called do it yourself education Get one of the books they give you to order from and learn from it. It always tells you what each antibody stains for, whats the control etc You actually can go online to any of the major sites such as DAKO, LEICA or VENTANA and see thier antibody list with all the applied info for them. In America you can also obtain knowledge from the NSH( http://www.nsh.org/ ) or as in Florida we have FSH (http://www.fshgroup.org/ ) Much Luck Kim D ________________________________ From: naveeda arshad To: histonet@lists.utsouthwestern.edu Sent: Sunday, January 8, 2012 4:16 PM Subject: [Histonet] Re:ihc course does any one know about institute offering IHC coursethanks --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 10 To: histonet@lists.utsouthwestern.edu Received: Sunday, January 8, 2012, 4:16 PM ------------------------------ Message: 5 Date: Mon, 09 Jan 2012 09:25:01 -0400 From: "Elizabeth Chatfield" Subject: [Histonet] Stability of Retic stain in Bone Marrow To: Message-ID: <4F0AB26D020000BC00014C4C@coregwia.peigov> Content-Type: text/plain; charset=US-ASCII Hi Everyone, I'm wondering if anyone has experienced issues with the stability of reticulin (Gordon and Sweets method) in bone marrow. We are seeing silver precipitant after ~1 week on our patient sections - our control is fine. Cheers, Elizabeth Peyton-Chatfield Histology Supervisor Queen Elizabeth Hospital Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ------------------------------ Message: 6 Date: Mon, 9 Jan 2012 09:32:12 -0600 From: "Genest, Sharon SktnHR" Subject: [Histonet] Respirators and Routine Histology To: Message-ID: Content-Type: text/plain; charset="us-ascii" Amy We use respirators when changing stainers and processors. Our Gross Technologists work in chemical hoods, our special stains and coverslipping are performed in chemical hoods. We anually measure air quality and our hood air flow. Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca ------------------------------ Message: 7 Date: Mon, 9 Jan 2012 09:36:10 -0600 From: "Genest, Sharon SktnHR" Subject: [Histonet] Electron Microscopy Proficiency testing To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone subscribe to a program for proficiency testing for Electron Microscopy? Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca ------------------------------ Message: 8 Date: Mon, 9 Jan 2012 10:44:14 -0500 From: Lorraine Cornett Subject: [Histonet] Controls To: Histonet Listserve Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are in need of Aspergillus and Amyloid controls. Does anyone on the histonet have any suggestions, or overabundance that they could share with us? Thanks, Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 ------------------------------ Message: 9 Date: Mon, 9 Jan 2012 10:00:48 -0600 From: "Nacaela Johnson" Subject: RE: [Histonet] Stability of Retic stain in Bone Marrow To: "'Elizabeth Chatfield'" , Message-ID: <000101cccee7$db9e1780$92da4680$@com> Content-Type: text/plain; charset="US-ASCII" My experience with the Gordon and Sweets Reticulin is 1 to 2 days max. I found better results and stability with the Gomoris Retic from Poly Scientific. Nacaela Johnson, B.S. HTL (ASCP) Histology Supervisor Kansas City Skin & Cancer Center, LLC 5810 NW Barry Rd, Ste 100 Kansas City, MO 64154 ph: 816 584 8100 fx: 816 584 8106 em: njohnson@kcskincenter.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Monday, January 09, 2012 7:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stability of Retic stain in Bone Marrow Hi Everyone, I'm wondering if anyone has experienced issues with the stability of reticulin (Gordon and Sweets method) in bone marrow. We are seeing silver precipitant after ~1 week on our patient sections - our control is fine. Cheers, Elizabeth Peyton-Chatfield Histology Supervisor Queen Elizabeth Hospital Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 9 Jan 2012 11:55:34 -0500 From: Judi Bennett Subject: [Histonet] Histology Authors Needed! To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 *HISTOLOGY* authors needed ... especially for *MOLECULAR* courses! Actively seeking authors to write online *histology courses* for *MediaLab*! MediaLab is a leading publisher of online continuing education (CE) courses and competency assessments. Our online products are used at more than 2,000 laboratories and university CLS programs worldwide. This is a great opportunity to: - Gain* resume-boosting publishing experience* - *Earn honorariums* for your participation - Fill the *critical need for quality histology CE credits* Authors can take advantage of MediaLab's online CourseBuilder to *write courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive interface similar to Microsoft Word or PowerPoint. Authors can quickly create content pages, practice questions, and exam questions, and upload relevant images. Courses developed by MediaLab are *featured on our websites MediaLabInc.net and LabCE.com*. Questions from these courses also become part of the LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers may also *contribute to other online programs that we develop on behalf of major laboratory partners*. To learn more about becoming a MediaLab author for histology courses, visit our online information page at www.medialbinc.net/authors.aspx . Please contact Judi Bennett at judi@medialabinc.net or call 877-776-8460, ext. 721. Judi Bennett Program Director, MediaLab, Inc. -- Judi Bennett, BSM, MT MediaLab, Inc. e-mail judi@medialabinc.net Phone (877) 776-8460 ext. 721 cell phone 404-915-2999 fax (678) 401-0284 ------------------------------ Message: 11 Date: Mon, 9 Jan 2012 11:25:52 -0600 From: Jan Shivers Subject: Re: [Histonet] Electron Microscopy Proficiency testing To: "Genest, Sharon SktnHR" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We participate in the External Quality Assessment Scheme in EM Virus Diagnostics (EQA-EMV) administered through the Robert Koch Institut of Berlin, Germany. It tests proficiency only on infectious disease identification. Jan Shivers Section Head - EM University of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN, USA On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR < Sharon.Genest@saskatoonhealthregion.ca> wrote: > Does anyone subscribe to a program for proficiency testing for Electron > Microscopy? > > Sharon Genest > Anatomic Pathology > Process Improvement > Saskatoon Health Region > 306-655-8242 > sharon.genest@saskatoonhealthregion.ca > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 11 **************************************** From Ronald.Houston <@t> nationwidechildrens.org Mon Jan 9 12:14:09 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Jan 9 12:14:24 2012 Subject: [Histonet] Electron Microscopy Proficiency testing In-Reply-To: References: Message-ID: We participate in the EQA Platelet Dense Granule Proficiency Scheme offered through NASCOLA (www.nascola.com). It tests proficiency only in dense granule platelet deficiency analysis by EM. I am not aware of any other EQA programs in EM, but you may be able to get some further information from the Microscopy Society of America http://www.microscopy.org Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Monday, January 09, 2012 12:26 PM To: Genest, Sharon SktnHR Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Electron Microscopy Proficiency testing We participate in the External Quality Assessment Scheme in EM Virus Diagnostics (EQA-EMV) administered through the Robert Koch Institut of Berlin, Germany. It tests proficiency only on infectious disease identification. Jan Shivers Section Head - EM University of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN, USA On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR < Sharon.Genest@saskatoonhealthregion.ca> wrote: > Does anyone subscribe to a program for proficiency testing for > Electron Microscopy? > > Sharon Genest > Anatomic Pathology > Process Improvement > Saskatoon Health Region > 306-655-8242 > sharon.genest@saskatoonhealthregion.ca > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jcox90 <@t> yahoo.com Mon Jan 9 12:19:54 2012 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Mon Jan 9 12:20:00 2012 Subject: [Histonet] Looking for part-time Histology position in Phoenix AZ area Message-ID: <1326133194.13589.YahooMailNeo@web161606.mail.bf1.yahoo.com> Hi Netters, I am looking for part time Histology work in the Phoenix area, I am an HT and have 17 years experience. Please respond for more details..Thanks!!? ? Jill Cox, HT ASCP From hfedor <@t> jhmi.edu Mon Jan 9 12:36:43 2012 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Jan 9 12:36:51 2012 Subject: [Histonet] Casual Temp Position Message-ID: Hello, We have a position available for a casual temp employee. This is a reference lab that primarily focuses on animal specimens. We will consider someone who has 2 years or more cutting experience. Please forward your resume to me. thanks Helen 410.614.1660 http://tmalab.jhmi.edu/ From hfedor <@t> jhmi.edu Mon Jan 9 13:12:31 2012 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Jan 9 13:12:38 2012 Subject: [Histonet] RE: Casual Temp Position In-Reply-To: References: Message-ID: Hello, We have a position available for a casual temp employee. This is a reference lab located in Baltimore MD, that primarily focuses on animal specimens. We will consider someone who has 2 years or more cutting experience. Please forward your resume to me. thanks Helen 410.614.1660 http://tmalab.jhmi.edu/ Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Monday, January 09, 2012 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Casual Temp Position Hello, We have a position available for a casual temp employee. This is a reference lab that primarily focuses on animal specimens. We will consider someone who has 2 years or more cutting experience. Please forward your resume to me. thanks Helen 410.614.1660 http://tmalab.jhmi.edu/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.kap.1 <@t> erasmusmc.nl Mon Jan 9 13:58:39 2012 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Mon Jan 9 13:58:57 2012 Subject: [Histonet] IHC quality program In-Reply-To: References: Message-ID: <3fa54a9ccef2381ea44d4ab8ee4d1642.squirrel@webmail.erasmusmc.nl> Dear all, We have developed CQPath for our QC/QA. Still under construction at www.cqpath.nl (of course also in English). Hopefully the TQM module will be available soon. Best regards, Marcel > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re:ihc course (naveeda arshad) > 2. Re: Re: Quality assurance program for pathologists (Kim Donadio) > 3. Re: Re:ihc course (Kim Donadio) > 4. Re: Re:ihc course (Lee & Peggy Wenk) > 5. Stability of Retic stain in Bone Marrow (Elizabeth Chatfield) > 6. Respirators and Routine Histology (Genest, Sharon SktnHR) > 7. Electron Microscopy Proficiency testing (Genest, Sharon SktnHR) > 8. Controls (Lorraine Cornett) > 9. RE: Stability of Retic stain in Bone Marrow (Nacaela Johnson) > 10. Histology Authors Needed! (Judi Bennett) > 11. Re: Electron Microscopy Proficiency testing (Jan Shivers) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 8 Jan 2012 13:16:58 -0800 (PST) > From: naveeda arshad > Subject: [Histonet] Re:ihc course > To: histonet@lists.utsouthwestern.edu > Message-ID: > <1326057418.98464.YahooMailClassic@web161706.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > does any one know?about?institute offering IHC coursethanks > --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu > wrote: > > From: histonet-request@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 98, Issue 10 > To: histonet@lists.utsouthwestern.edu > Received: Sunday, January 8, 2012, 4:16 PM > > Send Histonet mailing list submissions to > ??? histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ??? histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ??? histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ???1. Re: Quality assurance program for pathologists (Bob Richmond) > ???2. Re: finger nails (angela smith) > ???3. Re: finger nails (Michele Email) > ???4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey) > ???5. Advice needed the different types of cytomorphologic??? stains > ? ? ? (Gladys Lim) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 7 Jan 2012 13:40:26 -0500 > From: Bob Richmond > Subject: [Histonet] Re: Quality assurance program for pathologists > To: histonet@lists.utsouthwestern.edu > Message-ID: > ??? > Content-Type: text/plain; charset=ISO-8859-1 > > Diana McCaig (where?) asks: "Is anyone willing to share with me their > quality assurance/management > program for pathologists?" > > Glad to know you're in charge of pathologists. (Also glad I'm nearly > 73 years old, though not yet retired.) > > I've worked in a few programs that did a 10% review of cases. If you > go this route, don't choose the cases at random, but ask the > pathologists to designate the cases as they do the day's work - > they'll catch a lot more problems that way. I've worked in a single > practice that did 100% second-pathologist review (before the case was > released), and I thought that was excessive. > > Pathologists should be encouraged to document their internal > consultations - I mean when you pass a slide to the guy at the next > microscope and ask him "from the ear of a 70 year old man - do you > think there's enough here to call this a basal cell carcinoma?" Such > cases should be documented in a comment - I say "Dr. John Doe has seen > this material and concurs." Such cases are legitimately considered > part of a 10% review policy. I've worked in one large and highly > competent practice that documented internal consultation very > meticulously, and one of their QA guidelines was that 2.8% of their > cases document internal consultation. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 2 > Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST) > From: angela smith > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ??? ,??? Michele Carr > ??? , Rene J Buesa > Message-ID: > ??? <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Have you tried nairing it prior to fixation and processing?? We have > validated that applying a very thick coat of nair on the nail prior to > fixation for 15 min to 1 hour (depending on nail size and thickness)? then > rinse with tap, then place in formalin and process. We do not have any > issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > , "Michele Carr" > > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the > slide during staining.? The nail is extremely hard and seems to be washing > each time. Could I soften it prior to staining and what do you use to > soften it?? Thanks in advance for all your responses.? > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Sat, 7 Jan 2012 16:40:39 -0800 > From: Michele Email > Subject: Re: [Histonet] finger nails > To: angela smith > Cc: "Histonet@lists.utsouthwestern.edu" > ??? > Message-ID: > Content-Type: text/plain;??? charset=utf-8 > > Thanks everyone for the tips will try the Nair next time around. > Michele Carr > > Sent from my iPad > > On Jan 7, 2012, at 3:00 PM, angela smith wrote: > >> Have you tried nairing it prior to fixation and processing?? We have >> validated that applying a very thick coat of nair on the nail prior to >> fixation for 15 min to 1 hour (depending on nail size and thickness)? >> then rinse with tap, then place in formalin and process. We do not have >> any issues with nails falling off. Also make sure you use charged >> slides. >> >> --- On Fri, 1/6/12, Rene J Buesa wrote: >> >> From: Rene J Buesa >> Subject: Re: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> , "Michele Carr" >> >> Date: Friday, January 6, 2012, 3:45 PM >> >> See attachment! >> Ren?? J. >> >> --- On Fri, 1/6/12, Michele Carr wrote: >> >> >> From: Michele Carr >> Subject: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> >> Date: Friday, January 6, 2012, 1:06 PM >> >> >> Hi everyone was wondering what you do to get the nail from washing off >> the slide during staining.? The nail is extremely hard and seems to be >> washing each time. Could I soften it prior to staining and what do you >> use to soften it?? Thanks in advance for all your responses.? >> Michele Carr >> Medical Laboratory Services >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Sat, 7 Jan 2012 20:04:29 -0800 > From: Madeleine Huey > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 > To: histonet@lists.utsouthwestern.edu > Message-ID: > ??? > Content-Type: text/plain; charset=ISO-8859-1 > > Michele, > This is our procedure for toe & finger nails; > 1) Pre-fix nails in 10% NFB as usual > 2) Soak fixed nails in NAIR or any counter nail remover until soften > or bend easily (toe nail take longer; thickness dependent) > 3) wash nail with water > 4) Process in tissue processor > 5) embed & cut > Note; we found soften with NAIR before processing work the best. > > If NAIR is used after processing; > 1) cut nail on charge slides (+) > 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover > tightly > 3) bake jar in ~ 60c over for ~ 30 min > 4) cool & open jar in fume hood ~ 5 min > 5) stain as usual > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor - Pathology (IPOX & Histology) > madeleine_h@elcaminohospital.org > > > On Sat, Jan 7, 2012 at 10:00 AM, > wrote: >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. finger nails (Michele Carr) >> ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a >> ? ? ?Friend) (Cheryl) >> ? 3. Saffron (Gagnon, Eric) >> ? 4. Re: finger nails (Rene J Buesa) >> ? 5. Quality assurance program for pathologists (Diana McCaig) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) >> From: Michele Carr >> Subject: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hi everyone was wondering what you do to get the nail from washing off >> the slide during staining.? The nail is extremely hard and seems to be >> washing each time. Could I soften it prior to staining and what do you >> use to soften it?? Thanks in advance for all your responses. >> Michele Carr >> Medical Laboratory Services >> >> ------------------------------ >> >> Message: 2 >> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) >> From: Cheryl >> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for >> ? ? ? ?February/March ?(Refer a Friend) >> To: "histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hello 'Netters- >> >> Do you know any temp/travel PAs?? We've got a couple of different >> openings for the right people. In this economy who doesn't want a few >> more options??!!?? If you refer someone we help, you get a referral >> bonus (keep it or share it with your friend!) >> >> 1. Temp traveler for at least 4 weeks in mid-February. >> 2. Direct Hire in 6 different institutions around the US.? Entry through >> Supervisory. >> >> As a working tech I know a lot about the jobs before we submit you--and >> we respect that it's your life we're talking about--we help make sure >> you have lots of choices: you decide. >> >> Give me a call--make sure those you refer mention your name--I LIKE >> writing referral checks! >> >> Cheryl >> >> Cheryl Kerry, HT(ASCP) >> Full Staff Inc. >> Staffing the AP Lab by helping one GREAT?Tech at a time. >> 281.852.9457?Office >> 800.756.3309?Phone & Fax >> admin@fullstaff.org >> >> Sign up for the FREE?newsletter AP News--updates, tricks of the trade >> and current issues for Anatomic Pathology Clinical Labs. Send a >> 'subscribe' request to APNews@fullstaff.org. Please?include your name >> and specialty in the body of the email. >> >> ------------------------------ >> >> Message: 3 >> Date: Fri, 6 Jan 2012 15:19:18 -0500 >> From: "Gagnon, Eric" >> Subject: [Histonet] Saffron >> To: >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; ? ? ? charset="iso-8859-1" >> >> Beth, as Bob Richmond has noted regarding saffron, >> >> "The most common use is as the hematoxylin-phloxin-saffron (HPS) >> trichrome stain. It was in use as a general oversight stain in a few >> pathology services when I was a resident in the 1960's..." >> >> and is still in use here in Ontario by about 10% of the province's >> histology laboratories as a routine oversight stain. >> >> We have gone the same route as other respondents have noted over the >> years, utilizing a variety of suppliers, including a Mediterranean >> health food store in Ottawa for saffron. ?Now we are using the Sun Brand >> saffron produced in Spain, that is available at the check-out counter at >> our local bulk foods store. ?One might think that there would be a total >> shift to H&E as a routine stain, especially with automated stainers >> becoming prevalent, but we have successfully automated the stain on >> successive automated stainers. ?Since our newest pathologists were >> trained as residents here, they are quite used to HPS, and there appears >> to be little impetus to change. >> >> I still think the wafting of the boiling saffron is quite a pleasant >> aroma. >> >> Eric Gagnon MLT >> Histology Laboratory >> Kingston General Hospital >> Kingston, Ontario, Canada >> >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) >> From: Rene J Buesa >> Subject: Re: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> ? ? ? ?, ? ?Michele Carr >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> See attachment! >> Ren? J. >> >> --- On Fri, 1/6/12, Michele Carr wrote: >> >> >> From: Michele Carr >> Subject: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> >> Date: Friday, January 6, 2012, 1:06 PM >> >> >> Hi everyone was wondering what you do to get the nail from washing off >> the slide during staining.? The nail is extremely hard and seems to be >> washing each time. Could I soften it prior to staining and what do you >> use to soften it?? Thanks in advance for all your responses. >> Michele Carr >> Medical Laboratory Services >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 5 >> Date: Fri, 6 Jan 2012 17:06:19 -0500 >> From: "Diana McCaig" >> Subject: [Histonet] Quality assurance program for pathologists >> To: >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> Is anyone willing to share with me their quality assurance/management >> program for pathologists. >> >> >> >> Sincere thanks >> >> Diana >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 98, Issue 9 >> *************************************** > > > > ------------------------------ > > Message: 5 > Date: Mon, 9 Jan 2012 00:18:08 +0800 > From: Gladys Lim > Subject: [Histonet] Advice needed the different types of > ??? cytomorphologic??? stains > To: histonet@lists.utsouthwestern.edu > Message-ID: > ??? > Content-Type: text/plain; charset=ISO-8859-1 > > Dear all, > > I am relatively new to this area of staining approach and therefore, I > need > some advise on the different types of cytomorphologic stains that are > available. > > (1) Is it necessary to air-dry your sample prior to staining with any > Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? > (2) Has anyone tried using the Romanowski stains on sample that were not > air-dried? What was the outcome of the staining? > (3) Were there any distinct difference in terms of staining among the > different types of white blood cells vs. malignant cancer cells? > (4) Wouldn't air-drying of sample prior to Romanowski stain change the > morphology of cells? > (5) Any recommendation for other types of stains if I want to > differentiate > white blood cells from cancer cells, without any air-drying steps > involved? > > Any advice is greatly appreciated. > > Thanks. > > Regards, > > Gladys > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 10 > **************************************** > > > ------------------------------ > > Message: 2 > Date: Sun, 8 Jan 2012 14:27:29 -0800 (PST) > From: Kim Donadio > Subject: Re: [Histonet] Re: Quality assurance program for pathologists > To: Bob Richmond , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1326061649.24557.YahooMailNeo@web112301.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dr Richmond, > ?????????????????????? You always please me with your replies. You are > such a value to these forums. Let me, I'm sure for the thousant time tell > you how much you are appreciated. > ? > ? > You clearly understand "Quality" > ? > as most any Pathologist that Ive ever been subject to. > ? > As you know 10% review is the minumum > ? > 100%........... yep!~ Ive seen that.. what a marvel of respect did that > get from little ole me > ? > Yes, excessive, we both agree > ? > I have to say though to Diana, that usually the Pathologist have thier own > QA program that monitors thier work. Ive never seen anything different. > Even the lone Path. > ? > Its always a good idea to communicate with them > ? > They may already have the answer to your questions? > ? > Kim D > ? > > ? > > ________________________________ > From: Bob Richmond > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, January 7, 2012 1:40 PM > Subject: [Histonet] Re: Quality assurance program for pathologists > > Diana McCaig (where?) asks: "Is anyone willing to share with me their > quality assurance/management > program for pathologists?" > > Glad to know you're in charge of pathologists. (Also glad I'm nearly > 73 years old, though not yet retired.) > > I've worked in a few programs that did a 10% review of cases. If you > go this route, don't choose the cases at random, but ask the > pathologists to designate the cases as they do the day's work - > they'll catch a lot more problems that way. I've worked in a single > practice that did 100% second-pathologist review (before the case was > released), and I thought that was excessive. > > Pathologists should be encouraged to document their internal > consultations - I mean when you pass a slide to the guy at the next > microscope and ask him "from the ear of a 70 year old man - do you > think there's enough here to call this a basal cell carcinoma?" Such > cases should be documented in a comment - I say "Dr. John Doe has seen > this material and concurs." Such cases are legitimately considered > part of a 10% review policy. I've worked in one large and highly > competent practice that documented internal consultation very > meticulously, and one of their QA guidelines was that 2.8% of their > cases document internal consultation. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 3 > Date: Sun, 8 Jan 2012 14:40:46 -0800 (PST) > From: Kim Donadio > Subject: Re: [Histonet] Re:ihc course > To: naveeda arshad , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1326062446.12201.YahooMailNeo@web112306.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > You can get IHC education from most of the vendors. If you cant go that > route, then there are many good books and subscriber publications out. > ? > If you really want to be on top of the subject, do what I did many many > years ago. > ? > It's called do it yourself education > ? > Get one of the books they give you to order from and learn from it. It > always tells you what each antibody stains for, whats the control etc > ? > You actually can go online to any of the major sites such as DAKO, LEICA > or VENTANA and see thier antibody list with all the applied info for them. > ? > In America you can also obtain knowledge from the NSH( > http://www.nsh.org/?) or as in Florida we have FSH > (http://www.fshgroup.org/?) > ? > Much Luck > ? > Kim D > > > ________________________________ > From: naveeda arshad > To: histonet@lists.utsouthwestern.edu > Sent: Sunday, January 8, 2012 4:16 PM > Subject: [Histonet] Re:ihc course > > > does any one know?about?institute offering IHC coursethanks > --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu > wrote: > > From: histonet-request@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 98, Issue 10 > To: histonet@lists.utsouthwestern.edu > Received: Sunday, January 8, 2012, 4:16 PM > > Send Histonet mailing list submissions to > ??? histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ??? histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ??? histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ???1. Re: Quality assurance program for pathologists (Bob Richmond) > ???2. Re: finger nails (angela smith) > ???3. Re: finger nails (Michele Email) > ???4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey) > ???5. Advice needed the different types of cytomorphologic??? stains > ? ? ? (Gladys Lim) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 7 Jan 2012 13:40:26 -0500 > From: Bob Richmond > Subject: [Histonet] Re: Quality assurance program for pathologists > To: histonet@lists.utsouthwestern.edu > Message-ID: > ??? > Content-Type: text/plain; charset=ISO-8859-1 > > Diana McCaig (where?) asks: "Is anyone willing to share with me their > quality assurance/management > program for pathologists?" > > Glad to know you're in charge of pathologists. (Also glad I'm nearly > 73 years old, though not yet retired.) > > I've worked in a few programs that did a 10% review of cases. If you > go this route, don't choose the cases at random, but ask the > pathologists to designate the cases as they do the day's work - > they'll catch a lot more problems that way. I've worked in a single > practice that did 100% second-pathologist review (before the case was > released), and I thought that was excessive. > > Pathologists should be encouraged to document their internal > consultations - I mean when you pass a slide to the guy at the next > microscope and ask him "from the ear of a 70 year old man - do you > think there's enough here to call this a basal cell carcinoma?" Such > cases should be documented in a comment - I say "Dr. John Doe has seen > this material and concurs." Such cases are legitimately considered > part of a 10% review policy. I've worked in one large and highly > competent practice that documented internal consultation very > meticulously, and one of their QA guidelines was that 2.8% of their > cases document internal consultation. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 2 > Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST) > From: angela smith > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > ??? ,??? Michele Carr > ??? , Rene J Buesa > Message-ID: > ??? <1325977229.85027.YahooMailClassic@web125401.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Have you tried nairing it prior to fixation and processing?? We have > validated that applying a very thick coat of nair on the nail prior to > fixation for 15 min to 1 hour (depending on nail size and thickness)? then > rinse with tap, then place in formalin and process. We do not have any > issues with nails falling off. Also make sure you use charged slides. > > --- On Fri, 1/6/12, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > , "Michele Carr" > > Date: Friday, January 6, 2012, 3:45 PM > > See attachment! > Ren? J. > > --- On Fri, 1/6/12, Michele Carr wrote: > > > From: Michele Carr > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the > slide during staining.? The nail is extremely hard and seems to be washing > each time. Could I soften it prior to staining and what do you use to > soften it?? Thanks in advance for all your responses.? > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Sat, 7 Jan 2012 16:40:39 -0800 > From: Michele Email > Subject: Re: [Histonet] finger nails > To: angela smith > Cc: "Histonet@lists.utsouthwestern.edu" > ??? > Message-ID: > Content-Type: text/plain;??? charset=utf-8 > > Thanks everyone for the tips will try the Nair next time around. > Michele Carr > > Sent from my iPad > > On Jan 7, 2012, at 3:00 PM, angela smith wrote: > >> Have you tried nairing it prior to fixation and processing?? We have >> validated that applying a very thick coat of nair on the nail prior to >> fixation for 15 min to 1 hour (depending on nail size and thickness)? >> then rinse with tap, then place in formalin and process. We do not have >> any issues with nails falling off. Also make sure you use charged >> slides. >> >> --- On Fri, 1/6/12, Rene J Buesa wrote: >> >> From: Rene J Buesa >> Subject: Re: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> , "Michele Carr" >> >> Date: Friday, January 6, 2012, 3:45 PM >> >> See attachment! >> Ren?? J. >> >> --- On Fri, 1/6/12, Michele Carr wrote: >> >> >> From: Michele Carr >> Subject: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> >> Date: Friday, January 6, 2012, 1:06 PM >> >> >> Hi everyone was wondering what you do to get the nail from washing off >> the slide during staining.? The nail is extremely hard and seems to be >> washing each time. Could I soften it prior to staining and what do you >> use to soften it?? Thanks in advance for all your responses.? >> Michele Carr >> Medical Laboratory Services >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Sat, 7 Jan 2012 20:04:29 -0800 > From: Madeleine Huey > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9 > To: histonet@lists.utsouthwestern.edu > Message-ID: > ??? > Content-Type: text/plain; charset=ISO-8859-1 > > Michele, > This is our procedure for toe & finger nails; > 1) Pre-fix nails in 10% NFB as usual > 2) Soak fixed nails in NAIR or any counter nail remover until soften > or bend easily (toe nail take longer; thickness dependent) > 3) wash nail with water > 4) Process in tissue processor > 5) embed & cut > Note; we found soften with NAIR before processing work the best. > > If NAIR is used after processing; > 1) cut nail on charge slides (+) > 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover > tightly > 3) bake jar in ~ 60c over for ~ 30 min > 4) cool & open jar in fume hood ~ 5 min > 5) stain as usual > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor - Pathology (IPOX & Histology) > madeleine_h@elcaminohospital.org > > > On Sat, Jan 7, 2012 at 10:00 AM, > wrote: >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. finger nails (Michele Carr) >> ? 2. Temporary & Direct hire PA(ASCP) for February/March ? ? ? (Refer a >> ? ? ?Friend) (Cheryl) >> ? 3. Saffron (Gagnon, Eric) >> ? 4. Re: finger nails (Rene J Buesa) >> ? 5. Quality assurance program for pathologists (Diana McCaig) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) >> From: Michele Carr >> Subject: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1325873200.90291.YahooMailNeo@web120704.mail.ne1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hi everyone was wondering what you do to get the nail from washing off >> the slide during staining.? The nail is extremely hard and seems to be >> washing each time. Could I soften it prior to staining and what do you >> use to soften it?? Thanks in advance for all your responses. >> Michele Carr >> Medical Laboratory Services >> >> ------------------------------ >> >> Message: 2 >> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) >> From: Cheryl >> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for >> ? ? ? ?February/March ?(Refer a Friend) >> To: "histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1325875872.67239.YahooMailNeo@web39404.mail.mud.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hello 'Netters- >> >> Do you know any temp/travel PAs?? We've got a couple of different >> openings for the right people. In this economy who doesn't want a few >> more options??!!?? If you refer someone we help, you get a referral >> bonus (keep it or share it with your friend!) >> >> 1. Temp traveler for at least 4 weeks in mid-February. >> 2. Direct Hire in 6 different institutions around the US.? Entry through >> Supervisory. >> >> As a working tech I know a lot about the jobs before we submit you--and >> we respect that it's your life we're talking about--we help make sure >> you have lots of choices: you decide. >> >> Give me a call--make sure those you refer mention your name--I LIKE >> writing referral checks! >> >> Cheryl >> >> Cheryl Kerry, HT(ASCP) >> Full Staff Inc. >> Staffing the AP Lab by helping one GREAT?Tech at a time. >> 281.852.9457?Office >> 800.756.3309?Phone & Fax >> admin@fullstaff.org >> >> Sign up for the FREE?newsletter AP News--updates, tricks of the trade >> and current issues for Anatomic Pathology Clinical Labs. Send a >> 'subscribe' request to APNews@fullstaff.org. Please?include your name >> and specialty in the body of the email. >> >> ------------------------------ >> >> Message: 3 >> Date: Fri, 6 Jan 2012 15:19:18 -0500 >> From: "Gagnon, Eric" >> Subject: [Histonet] Saffron >> To: >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; ? ? ? charset="iso-8859-1" >> >> Beth, as Bob Richmond has noted regarding saffron, >> >> "The most common use is as the hematoxylin-phloxin-saffron (HPS) >> trichrome stain. It was in use as a general oversight stain in a few >> pathology services when I was a resident in the 1960's..." >> >> and is still in use here in Ontario by about 10% of the province's >> histology laboratories as a routine oversight stain. >> >> We have gone the same route as other respondents have noted over the >> years, utilizing a variety of suppliers, including a Mediterranean >> health food store in Ottawa for saffron. ?Now we are using the Sun Brand >> saffron produced in Spain, that is available at the check-out counter at >> our local bulk foods store. ?One might think that there would be a total >> shift to H&E as a routine stain, especially with automated stainers >> becoming prevalent, but we have successfully automated the stain on >> successive automated stainers. ?Since our newest pathologists were >> trained as residents here, they are quite used to HPS, and there appears >> to be little impetus to change. >> >> I still think the wafting of the boiling saffron is quite a pleasant >> aroma. >> >> Eric Gagnon MLT >> Histology Laboratory >> Kingston General Hospital >> Kingston, Ontario, Canada >> >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) >> From: Rene J Buesa >> Subject: Re: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> ? ? ? ?, ? ?Michele Carr >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1325882708.34952.YahooMailClassic@web65705.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> See attachment! >> Ren? J. >> >> --- On Fri, 1/6/12, Michele Carr wrote: >> >> >> From: Michele Carr >> Subject: [Histonet] finger nails >> To: "Histonet@lists.utsouthwestern.edu" >> >> Date: Friday, January 6, 2012, 1:06 PM >> >> >> Hi everyone was wondering what you do to get the nail from washing off >> the slide during staining.? The nail is extremely hard and seems to be >> washing each time. Could I soften it prior to staining and what do you >> use to soften it?? Thanks in advance for all your responses. >> Michele Carr >> Medical Laboratory Services >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 5 >> Date: Fri, 6 Jan 2012 17:06:19 -0500 >> From: "Diana McCaig" >> Subject: [Histonet] Quality assurance program for pathologists >> To: >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> Is anyone willing to share with me their quality assurance/management >> program for pathologists. >> >> >> >> Sincere thanks >> >> Diana >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 98, Issue 9 >> *************************************** > > > > ------------------------------ > > Message: 5 > Date: Mon, 9 Jan 2012 00:18:08 +0800 > From: Gladys Lim > Subject: [Histonet] Advice needed the different types of > ??? cytomorphologic??? stains > To: histonet@lists.utsouthwestern.edu > Message-ID: > ??? > Content-Type: text/plain; charset=ISO-8859-1 > > Dear all, > > I am relatively new to this area of staining approach and therefore, I > need > some advise on the different types of cytomorphologic stains that are > available. > > (1) Is it necessary to air-dry your sample prior to staining with any > Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)? > (2) Has anyone tried using the Romanowski stains on sample that were not > air-dried? What was the outcome of the staining? > (3) Were there any distinct difference in terms of staining among the > different types of white blood cells vs. malignant cancer cells? > (4) Wouldn't air-drying of sample prior to Romanowski stain change the > morphology of cells? > (5) Any recommendation for other types of stains if I want to > differentiate > white blood cells from cancer cells, without any air-drying steps > involved? > > Any advice is greatly appreciated. > > Thanks. > > Regards, > > Gladys > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 10 > **************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 4 > Date: Sun, 8 Jan 2012 23:32:05 -0500 > From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] Re:ihc course > To: "Kim Donadio" , "naveeda arshad" > , > Message-ID: > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > NSH has a lot of IHC teleconferences: > http://s3.goeshow.com/nsh/NSHTC2012/ereg949997.cfm?pg=home > Under Register, you can download the brochure. > > NSH also has some IHC forums: July 13-14, 2012 in Hartford, CT. Not much > information available yet, but keep checking. > http://www.nsh.org/content/immunohistochemistry-forum > > Dako has a lot of downloadable booklets > http://www.dako.com/us/index/knowledgecenter.htm > > But as for a college offering courses/degree in IHC - I don't know of any. > There are courses on Immunology in general, and there are courses for the > med tech training programs, specific to their immunology. For the > histotech > programs that are college/university based, the IHC is built into the > curriculum, usually part of the special stains class, and you have to be > accepted into the histotech program. So most histotechs learn it > on-the-job, > and attending their state and national symposiums. > > Peggy Wenk, HTL(ASCP)SLS > > -----Original Message----- > From: Kim Donadio > Sent: Sunday, January 08, 2012 5:40 PM > To: naveeda arshad ; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re:ihc course > > You can get IHC education from most of the vendors. If you cant go that > route, then there are many good books and subscriber publications out. > > If you really want to be on top of the subject, do what I did many many > years ago. > > It's called do it yourself education > > Get one of the books they give you to order from and learn from it. It > always tells you what each antibody stains for, whats the control etc > > You actually can go online to any of the major sites such as DAKO, LEICA > or > VENTANA and see thier antibody list with all the applied info for them. > > In America you can also obtain knowledge from the NSH( http://www.nsh.org/ > ) > or as in Florida we have FSH (http://www.fshgroup.org/ ) > > Much Luck > > Kim D > > > ________________________________ > From: naveeda arshad > To: histonet@lists.utsouthwestern.edu > Sent: Sunday, January 8, 2012 4:16 PM > Subject: [Histonet] Re:ihc course > > > does any one know about institute offering IHC coursethanks > --- On Sun, 1/8/12, histonet-request@lists.utsouthwestern.edu > wrote: > > From: histonet-request@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 98, Issue 10 > To: histonet@lists.utsouthwestern.edu > Received: Sunday, January 8, 2012, 4:16 PM > > > > > > ------------------------------ > > Message: 5 > Date: Mon, 09 Jan 2012 09:25:01 -0400 > From: "Elizabeth Chatfield" > Subject: [Histonet] Stability of Retic stain in Bone Marrow > To: > Message-ID: <4F0AB26D020000BC00014C4C@coregwia.peigov> > Content-Type: text/plain; charset=US-ASCII > > Hi Everyone, > > I'm wondering if anyone has experienced issues with the stability of > reticulin (Gordon and Sweets method) in bone marrow. We are seeing > silver precipitant after ~1 week on our patient sections - our control is > fine. > > Cheers, > > Elizabeth Peyton-Chatfield > Histology Supervisor > Queen Elizabeth Hospital > Charlottetown, PE > > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or organization. > If you have received this communication in error, please notify the sender > immediately. If you are not the intended recipient, you are not authorized > to use, disclose, distribute, copy, print or rely on this email, and > should promptly delete this email from your entire computer system. > > > > > > > ------------------------------ > > Message: 6 > Date: Mon, 9 Jan 2012 09:32:12 -0600 > From: "Genest, Sharon SktnHR" > > Subject: [Histonet] Respirators and Routine Histology > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Amy > We use respirators when changing stainers and processors. Our Gross > Technologists work in chemical hoods, our special stains and > coverslipping are performed in chemical hoods. We anually measure air > quality and our hood air flow. > > Sharon Genest > Anatomic Pathology > Process Improvement > Saskatoon Health Region > 306-655-8242 > sharon.genest@saskatoonhealthregion.ca > > > > ------------------------------ > > Message: 7 > Date: Mon, 9 Jan 2012 09:36:10 -0600 > From: "Genest, Sharon SktnHR" > > Subject: [Histonet] Electron Microscopy Proficiency testing > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Does anyone subscribe to a program for proficiency testing for Electron > Microscopy? > > Sharon Genest > Anatomic Pathology > Process Improvement > Saskatoon Health Region > 306-655-8242 > sharon.genest@saskatoonhealthregion.ca > > > > ------------------------------ > > Message: 8 > Date: Mon, 9 Jan 2012 10:44:14 -0500 > From: Lorraine Cornett > Subject: [Histonet] Controls > To: Histonet Listserve > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > We are in need of Aspergillus and Amyloid controls. Does anyone on the > histonet have any suggestions, or overabundance that they could share with > us? > > Thanks, > > Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, > Kingsport, TN 423 224-5793 fax 423 224-5349 > > ------------------------------ > > Message: 9 > Date: Mon, 9 Jan 2012 10:00:48 -0600 > From: "Nacaela Johnson" > Subject: RE: [Histonet] Stability of Retic stain in Bone Marrow > To: "'Elizabeth Chatfield'" , > > Message-ID: <000101cccee7$db9e1780$92da4680$@com> > Content-Type: text/plain; charset="US-ASCII" > > My experience with the Gordon and Sweets Reticulin is 1 to 2 days max. I > found better results and stability with the Gomoris Retic from Poly > Scientific. > > Nacaela Johnson, B.S. HTL (ASCP) > Histology Supervisor > Kansas City Skin & Cancer Center, LLC > 5810 NW Barry Rd, Ste 100 > Kansas City, MO 64154 > ph: 816 584 8100 > fx: 816 584 8106 > em: njohnson@kcskincenter.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth > Chatfield > Sent: Monday, January 09, 2012 7:25 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Stability of Retic stain in Bone Marrow > > Hi Everyone, > > I'm wondering if anyone has experienced issues with the stability of > reticulin (Gordon and Sweets method) in bone marrow. We are seeing > silver > precipitant after ~1 week on our patient sections - our control is fine. > > Cheers, > > Elizabeth Peyton-Chatfield > Histology Supervisor > Queen Elizabeth Hospital > Charlottetown, PE > > > ------------------------- > > Statement of Confidentiality > > This message (including attachments) may contain confidential or > privileged > information intended for a specific individual or organization. If you > have > received this communication in error, please notify the sender > immediately. > If you are not the intended recipient, you are not authorized to use, > disclose, distribute, copy, print or rely on this email, and should > promptly > delete this email from your entire computer system. > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Mon, 9 Jan 2012 11:55:34 -0500 > From: Judi Bennett > Subject: [Histonet] Histology Authors Needed! > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=windows-1252 > > *HISTOLOGY* authors needed especially for *MOLECULAR* courses! > > > Actively seeking authors to write online *histology courses* for > *MediaLab*! > MediaLab is a leading publisher of online continuing education (CE) > courses > and competency assessments. Our online products are used at more than > 2,000 > laboratories and university CLS programs worldwide. > > > This is a great opportunity to: > > > - Gain* resume-boosting publishing experience* > - *Earn honorariums* for your participation > - Fill the *critical need for quality histology CE credits* > > > Authors can take advantage of MediaLab's online CourseBuilder to *write > courses anytime, anywhere*. CourseBuilder is easy to use, with an > intuitive > interface similar to Microsoft Word or PowerPoint. Authors can quickly > create content pages, practice questions, and exam questions, and upload > relevant images. > > > Courses developed by MediaLab are *featured on our websites > MediaLabInc.net > and LabCE.com*. Questions from these courses also become part of the > LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers > may also *contribute to other online programs that we develop on behalf of > major laboratory partners*. > > > To learn more about becoming a MediaLab author for histology courses, > visit > our online information page at www.medialbinc.net/authors.aspx . Please > contact Judi Bennett at judi@medialabinc.net or call 877-776-8460, ext. > 721. > > > Judi Bennett > > Program Director, MediaLab, Inc. > > -- > > Judi Bennett, BSM, MT > > MediaLab, Inc. > e-mail judi@medialabinc.net > Phone (877) 776-8460 ext. 721 > > cell phone 404-915-2999 > fax (678) 401-0284 > > > ------------------------------ > > Message: 11 > Date: Mon, 9 Jan 2012 11:25:52 -0600 > From: Jan Shivers > Subject: Re: [Histonet] Electron Microscopy Proficiency testing > To: "Genest, Sharon SktnHR" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > We participate in the External Quality Assessment Scheme in EM Virus > Diagnostics (EQA-EMV) administered through the Robert Koch Institut of > Berlin, Germany. It tests proficiency only on infectious disease > identification. > > Jan Shivers > Section Head - EM > University of Minnesota > Veterinary Diagnostic Laboratory > St. Paul, MN, USA > > On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR < > Sharon.Genest@saskatoonhealthregion.ca> wrote: > >> Does anyone subscribe to a program for proficiency testing for Electron >> Microscopy? >> >> Sharon Genest >> Anatomic Pathology >> Process Improvement >> Saskatoon Health Region >> 306-655-8242 >> sharon.genest@saskatoonhealthregion.ca >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 11 > **************************************** > From TJohnson <@t> gnf.org Mon Jan 9 14:19:33 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Mon Jan 9 14:19:39 2012 Subject: [Histonet] Digest users, please read Message-ID: <9F3CFEE76E51B64991C7485270890B4009EE31CA@EX4.lj.gnf.org> I'm dying here. Histonet users, if you have the digest open and want to reply to a post, please do the following if you click "Reply": Change the Subject from Re: Histonet Digest, Vol xx, Issue xx to Re: whatever subject you are responding to Go to the body of the email, Highlight all the text here and delete it. This can be done by using Ctrl+A (Cmd+A for Mac) and then pressing the delete key. Now type in your response and send. You can also do what I do, find the post of interest in the body of the email you are responding to, then click on New email which opens in a new window. Address it to the histonet appropriately. In the subject line, type in Re: and then type in or copy/paste the subject you are addressing. In the body of the email, type to your heart's content and click send. We digest users get bombarded with the same digests repeated over and over and it makes it very difficult to find the new topics. Thanks for your help. The last couple have been ridiculous. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From SAllen <@t> dsmanitoba.ca Mon Jan 9 15:26:00 2012 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Mon Jan 9 15:26:58 2012 Subject: [Histonet] FW: standard for sm bx microtomy Message-ID: Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From SAllen <@t> dsmanitoba.ca Mon Jan 9 15:27:43 2012 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Mon Jan 9 15:28:35 2012 Subject: [Histonet] FW: Quality Modules Message-ID: Hi, I am looking for information on Quality Modules that can be added into an existing IT system and system with great Quality Modules built into their IT platform. Thanks for any information. Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From amber.mckenzie <@t> gastrodocs.net Mon Jan 9 15:44:15 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Jan 9 15:42:50 2012 Subject: [Histonet] RE: standard for sm bx microtomy In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC13B715@JERRY.Gia.com> We are a GI lab and I do 3 levels on each block, 3 sections on each slide -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Monday, January 09, 2012 3:26 PM To: histonet@lists.utsouthwestern.edu Cc: Lisa Manning Subject: [Histonet] FW: standard for sm bx microtomy Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca From Richard.Breckenridge <@t> uth.tmc.edu Mon Jan 9 15:46:17 2012 From: Richard.Breckenridge <@t> uth.tmc.edu (Breckenridge, Richard A) Date: Mon Jan 9 15:46:22 2012 Subject: [Histonet] Adenovirus + control slides Message-ID: <3FE6D14CF4B4174AA4783A50F58CD30D0F1F97B78E@UTHCMS3.uthouston.edu> Can anyone direct me to a vendor for FFPE Adenovirus + control slides. We have exhausted our in house control tissue. Thanks everyone!! Richard A. Breckenridge, HT(ASCP) Histology Laboratory Manager University of Texas Health Science Center at Houston Medical School Building 2.234 ph. 713-500-6792 fax 713-500-0733 richard.breckenridge@uth.tmc.edu From kkienitz <@t> orclinic.com Mon Jan 9 16:04:05 2012 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Mon Jan 9 16:08:51 2012 Subject: [Histonet] RE: standard for sm bx microtomy In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC13B715@JERRY.Gia.com> References: , <5A33C952BB67F4468AF1F36D739212BC13B715@JERRY.Gia.com> Message-ID: <41400FFE517878449D89114DD2526090031ACCF46F@tocmail1.tocad.orclinic.com> Do you have a maximum number of pieces of tissue per block? I work for a GI lab also. My slides go out to a hospital path group who insist only 5 pieces of tissue per block. Of course this adds a lot of extra work on many cases...just wondering? Kari Kienitz, HT(ASCP) The Oregon Clinic - Portland GI ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Monday, January 09, 2012 1:44 PM To: Sharon Allen; histonet@lists.utsouthwestern.edu Cc: Lisa Manning Subject: [Histonet] RE: standard for sm bx microtomy We are a GI lab and I do 3 levels on each block, 3 sections on each slide -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Monday, January 09, 2012 3:26 PM To: histonet@lists.utsouthwestern.edu Cc: Lisa Manning Subject: [Histonet] FW: standard for sm bx microtomy Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yolanda.davies <@t> uct.ac.za Mon Jan 9 06:13:29 2012 From: yolanda.davies <@t> uct.ac.za (Yolanda Davies) Date: Mon Jan 9 23:06:17 2012 Subject: [Histonet] bromoform ingestion Message-ID: <4F0AF60902000022000AB51B@gwiasmtp.uct.ac.za> Dear All Is it possible to demonstrate the presence of bromoform within gastro-intestinal tissues in histology? Bromoform is similar to chloroform, the anaesthetic agent, except with 3 bromine instead of chlorine atoms in the molecule and is clearly demonstrated by means of x-rays. All we know is that bromine is very reactive because it is a halogen being highly electronegative, so this makes us think that perhaps it would not be wise to place the tissues into formalin and so on, but rather to perform the test on fresh tissue. Your advice would be greatly appreciated. From Tony_Reilly <@t> health.qld.gov.au Mon Jan 9 23:19:16 2012 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Mon Jan 9 23:19:50 2012 Subject: [Histonet] Electron Microscopy Proficiency testing In-Reply-To: References: Message-ID: <4F0C56F5.411C.0039.0@health.qld.gov.au> Hello Sharon The Royal College of Pathologists Quality Assurance Program (RCPA QAP) runs a transmission EM module as part of the Anatomical Pathology program. Overseas organisations are eligible to join and there are currently many subscribers from SE Asia and the Middle East. Their website is : www.rcpaqap.com.au ( http://www.rcpaqap.com.au/ ) All the best Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Genest, Sharon SktnHR" 1/10/2012 1:36 am >>> Does anyone subscribe to a program for proficiency testing for Electron Microscopy? Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From sdysart <@t> mirnarx.com Tue Jan 10 09:15:40 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Jan 10 09:15:51 2012 Subject: [Histonet] Test Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D509C9CBC@SN2PRD0702MB098.namprd07.prod.outlook.com> Hmm Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From TillRenee <@t> uams.edu Tue Jan 10 10:21:54 2012 From: TillRenee <@t> uams.edu (Till, Renee) Date: Tue Jan 10 10:21:25 2012 Subject: [Histonet] CD marker help [particularly CD68] Message-ID: Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I am focusing on the CD68 for now)on some mouse adipose tissue and have been getting varying results. I don't have any experience working with CD marker or doing IHC on adipose tissue. Without getting into the whole long process I have had trying to optimize this antibody, I am hoping someone can answer a couple of questions for me. 1. Can CD marker, and CD68 in particular, be picky as far as needing fresh cut FFPE slides? With everything I have tried, this is the only thing I can come up with for the inconsistencies I have experienced. 2. Positive control. I do not have one. Any suggestions? At least I do not have a positive control adipose block. I have tried mouse spleen, lung, and duo, and all have worked, but not consistently, and not in keeping with my adipose test slides. I can have beautiful stained spleen and duo, but my adipose slide will be completely brown. I believe I have about worked this issue out....as far as getting good staining on the fat and the control tissues, but then I encounter what led to my question #1. Renee' Fox, HT (ASCP) Research Assistant Arkansas Children's Nutrition Center 15 Children's Way./N2021 Little Rock, AR 72202 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From jpgrock37 <@t> yahoo.com Tue Jan 10 11:27:54 2012 From: jpgrock37 <@t> yahoo.com (Jessica Piche) Date: Tue Jan 10 11:28:09 2012 Subject: [Histonet] Cryostats Message-ID: <1326216474.29451.YahooMailNeo@web121502.mail.ne1.yahoo.com> Hi Everyone, ? We are looking for a new cryostat and are wondering what everyone has, how they like them, what they recommend, etc! Thank you, ? Jessica Piche-Grocki, HT(ASCP) From MDiCarlo <@t> KaleidaHealth.Org Tue Jan 10 11:35:03 2012 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Tue Jan 10 11:36:38 2012 Subject: [Histonet] difficult cross section to cut Message-ID: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org> Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From relia1 <@t> earthlink.net Tue Jan 10 11:42:15 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 10 11:42:18 2012 Subject: [Histonet] RELIA HOT HOT HOT Histology Job Alert - 01/10/2012 Run your own private speciality lab in Atlanta, GA Message-ID: Hi Histonetters! I hope everyone is having a great day! I have a new position that I am pretty excited about. This opportunity is with a private specialty lab in Atlanta, GA. This is a full time permanent position and my client offers excellent pay and benefits. They are looking for someone who is ASCP certified and has at least 5 years of histology experience grossing and IHC is a plus. Unfortunately they are not willing to pay for relocation expenses. If you or anyone you know might be interested in hearing about this opportunity please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Thanks and Have a Great Day!! >Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From leiker <@t> buffalo.edu Tue Jan 10 12:46:34 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Tue Jan 10 12:46:42 2012 Subject: [Histonet] stripping antibodies Message-ID: Anyone know a buffer or method (not using heat) to strip antibodies off cells used in an immunocytostaining procedure? The cells are in a multi-well tissue culture dish and have only been labeled with primary (unconjugated) antibody. Thanks. Merced M Leiker Cardiovascular Medicine Biomedical Research Building Rm 348 State University of New York at Buffalo 3435 Main St., Buffalo, NY 14214 (Ph) 716-829-6118 (Fx) 716-829-2665 From careerstudio <@t> bellsouth.net Tue Jan 10 13:40:57 2012 From: careerstudio <@t> bellsouth.net (Career Studio) Date: Tue Jan 10 13:40:50 2012 Subject: [Histonet] Position Opening: Clinical laboratory based in the Trumbull, CT area seeking an experienced Histotechnician Message-ID: <08fa01cccfcf$c684e5c0$538eb140$@net> Fine clinical laboratory based in the Trumbull, CT area currently seeking an experienced Histotechnician to perform routine & non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s). The shift is Monday ? Friday, 8a-5p. Accountabilities will be grossing, embedding, microtomy, sign-out, IHC/special stains; bench work to ensure timely completion of work; proper tissue processing; quality control activity documentation; differentiation of acceptable and unacceptable processing, embedding, cutting, & staining of tissue specimens Specific duties include: ?Ensure proper accessioning and labeling of all tissue samples & proper tissue processing. ?Process paperwork associated with accessioning & reporting. ?Embed processed tissue in paraffin. ?Perform microtomy of embedded tissue. ?Prepare slides for routine Hematoxylin & Eosin staining. ?Perform coverslipping of stained slides either manually or automated. ?Prepare solutions and reagents for special stain procedures. ?Obtain & validate tissue used in special stains. ?Perform all special stain procedures. ?May prepare solutions & reagents for IHC procedures. ?May obtain & validate control material used in IHC procedures & perform IHC testing ?Perform filing of finished blocks & slides. ?Handle routine maintenance / cleaning of equipment & troubleshoot minor equipment failures. ?Document remedial actions such as repairs or repeated tests. ?Provide training & guidance to Histotechnicians, students and lab aides. ?Ensure all corporate safety, quality control & quality assurance standards are met. ?Maintain compliance with all local, federal, CLIA & CAP regulations. Requirements : Minimum of Associates degree with appropriate coursework, 2 years of histology exp; HT or HTL ASCP . Immunohistochemistry (IHC) background desired.. This opportunity offers excellent salary (commensurate with exp), annual bonuses, relocation assistance & rewarding career path. Please do contact me at biolabcareers@aol.com to share your employment goals for the New Year. David King CAREER STUDIO ~ Biotechnology division http://www.linkedin.com/in/biotechnologyhires From rjbuesa <@t> yahoo.com Tue Jan 10 14:33:26 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 10 14:33:31 2012 Subject: [Histonet] difficult cross section to cut In-Reply-To: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org> Message-ID: <1326227606.70678.YahooMailClassic@web65712.mail.ac4.yahoo.com> Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10?, go to 30? angle. Ren? J. --- On Tue, 1/10/12, DiCarlo, Margaret wrote: From: DiCarlo, Margaret Subject: [Histonet] difficult cross section to cut To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 10, 2012, 12:35 PM Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic.? After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section.? The knife just skips over the bone.? I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions?? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY? 14203 716-859-1293 The Keeping You Informed section of Kaleida Health?s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Tue Jan 10 14:45:23 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Tue Jan 10 14:45:49 2012 Subject: [Histonet] Re: Digest users please read In-Reply-To: References: Message-ID: <003001cccfd8$c6a59e10$53f0da30$@com> Thank you thank you! Have a great day everyone! From cpyse <@t> x-celllab.com Tue Jan 10 15:19:49 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Jan 10 15:19:47 2012 Subject: [Histonet] bleaching melanin Message-ID: <004d01cccfdd$96538600$c2fa9200$@com> Hello Histonetters I need to bleach melanin out of some paraffin sections. The only bleaching solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2 solution for 1 to 2 days. How long do you think it will take to bleach the section in a 3% solution? Of course everyone wants it yesterday or I could order the K permanganate and oxalic acid I really need. Any suggestions would be greatly appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com From equineshowmom01 <@t> yahoo.com Tue Jan 10 15:21:37 2012 From: equineshowmom01 <@t> yahoo.com (Sheree H) Date: Tue Jan 10 15:21:41 2012 Subject: [Histonet] (no subject) Message-ID: <1326230497.25128.YahooMailMobile@web125802.mail.ne1.yahoo.com> http://dickcolewatercolors.com/images/3rd-level-pages/xmas.php?sound128.jpg From cbarone <@t> NEMOURS.ORG Tue Jan 10 15:46:23 2012 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Jan 10 15:46:49 2012 Subject: [Histonet] Sodium Barbital Message-ID: Histonetters, Anyone out there still doing ATPase's on muscles these days? Where are you ordering your sodium barbital from? We have always used Spectrum. They no longer carry it. If we cannot find another vendor, is there an alternate protocol someone might share with us? Thanks guys! From jshelley <@t> sanfordburnham.org Tue Jan 10 16:02:25 2012 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Jan 10 16:03:20 2012 Subject: [Histonet] RE: Sodium Barbital In-Reply-To: References: Message-ID: <5A605CE38EECB64B94485C02125A0C44060F5C2F45@LN-MAIL07.ln.burnham.org> Hi Carol, I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! Kind Regards! ? John J Shelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Tuesday, January 10, 2012 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sodium Barbital Importance: High Histonetters, Anyone out there still doing ATPase's on muscles these days? Where are you ordering your sodium barbital from? We have always used Spectrum. They no longer carry it. If we cannot find another vendor, is there an alternate protocol someone might share with us? Thanks guys! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Tue Jan 10 16:06:48 2012 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Jan 10 16:06:54 2012 Subject: [Histonet] Quality Management Monitors Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092@EXCHANGE2K7.staprimecare.org> In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all. Thank you in advance for any ideas that you may be able to share! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From daniel.sjolander <@t> gmail.com Tue Jan 10 16:07:54 2012 From: daniel.sjolander <@t> gmail.com (Daniel Sjolander) Date: Tue Jan 10 16:08:28 2012 Subject: [Histonet] RE: Sodium Barbital In-Reply-To: <5A605CE38EECB64B94485C02125A0C44060F5C2F45@LN-MAIL07.ln.burnham.org> References: <5A605CE38EECB64B94485C02125A0C44060F5C2F45@LN-MAIL07.ln.burnham.org> Message-ID: Tried Sigma-Aldrich/Fluka? /D On Tue, Jan 10, 2012 at 11:02 PM, John Shelley wrote: > Hi Carol, > > I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! > > Kind Regards! > > John J Shelley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol > Sent: Tuesday, January 10, 2012 4:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sodium Barbital > Importance: High > > Histonetters, > Anyone out there still doing ATPase's on muscles these days? Where are > you ordering your sodium barbital from? We have always used Spectrum. > They no longer carry it. If we cannot find another vendor, is there an > alternate protocol someone might share with us? Thanks guys! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jan 10 16:18:52 2012 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Tue Jan 10 16:18:56 2012 Subject: [Histonet] Quality Management Monitors Message-ID: I have used QA feedback/documentation/randomized block and slide audit. Would include occurence, root cause, technical variables, patient impact, correction, training, competency etc. I would think you develop scaling like FMEA maybe for subjective items. The pathologist feedback is always a good source.You can use manual paper or LIS to track, then trend in excel. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Knutson Deanne Date: Tue, 10 Jan 2012 22:06:48 To: Subject: [Histonet] Quality Management Monitors In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality?? I am not talking productivity, but quality.? How can we monitor these benches to help staff improve the quality of their work?? I don't want this to be punitive, but a learning experience for all. Thank you in advance for any ideas that you may be able to share! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sowmyakedarnath <@t> gmail.com Tue Jan 10 16:25:12 2012 From: sowmyakedarnath <@t> gmail.com (Sowmya Kedarnath) Date: Tue Jan 10 16:25:17 2012 Subject: [Histonet] Used Tissue Embedding Station Message-ID: Hello Histonetters, I am looking for a used Tissue Embedding Station.If anyone is interested in selling ,kindly contact me. Thank You Regards Sowmya Kedarnath From Eric.Hoy <@t> UTSouthwestern.edu Tue Jan 10 16:36:58 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Tue Jan 10 16:37:05 2012 Subject: [Histonet] Sodium Barbital In-Reply-To: <3f55b1c3b79b42d8b6569647789bf26c@SWMSHUB3.swmed.org> Message-ID: There is a substitute that has been around for a long time. I have used this substitute for immunoelectrophoresis and gotten good results. The reference is Clin Chem, 1978, 24(10):1825-1827. I can send a copy if you don't have access to the on-line journal. You might also search the archives. I remember a discussion about this several years ago, and I think Tony Henwood recommended an acetate buffer for ATPases. Happy staining! Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 1/10/12 3:46 PM, "Barone, Carol" wrote: > Histonetters, > Anyone out there still doing ATPase's on muscles these days? Where are > you ordering your sodium barbital from? We have always used Spectrum. > They no longer carry it. If we cannot find another vendor, is there an > alternate protocol someone might share with us? Thanks guys! From amosbrooks <@t> gmail.com Tue Jan 10 16:54:23 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jan 10 16:54:28 2012 Subject: [Histonet] CD marker help [particularly CD68] Message-ID: Hi, It sounds like there are a number of antibodies with similar problems here if you are having troubles with CD68, F4/80 and CD11b. If you are getting variable results it could be that the tissues are not fixed and processed sufficiently. If they are not fixed sufficiently there will be inconsistencies in labeling of the epitopes. Adipose tissues take particularly long to fix. In the absence of further details about the procedure, that is where I would assume the gremlin is hiding. Amos On Tue, Jan 10, 2012 at 1:00 PM, wrote: > Message: 10 > Date: Tue, 10 Jan 2012 16:21:54 +0000 > From: "Till, Renee" > Subject: [Histonet] CD marker help [particularly CD68] > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I > am focusing on the CD68 for now)on some mouse adipose tissue and have been > getting varying results. I don't have any experience working with CD marker > or doing IHC on adipose tissue. Without getting into the whole long process > I have had trying to optimize this antibody, I am hoping someone can answer > a couple of questions for me. > 1. Can CD marker, and CD68 in particular, be picky as far as needing > fresh cut FFPE slides? With everything I have tried, this is the only thing > I can come up with for the inconsistencies I have experienced. > 2. Positive control. I do not have one. Any suggestions? At least I do not > have a positive control adipose block. I have tried mouse spleen, lung, and > duo, and all have worked, but not consistently, and not in keeping with my > adipose test slides. I can have beautiful stained spleen and duo, but my > adipose slide will be completely brown. I believe I have about worked this > issue out....as far as getting good staining on the fat and the control > tissues, but then I encounter what led to my question #1. > > > Renee' Fox, HT (ASCP) > From kasaim <@t> mail.nih.gov Tue Jan 10 17:04:58 2012 From: kasaim <@t> mail.nih.gov (Kasai, Miki (NIH/NCI) [E]) Date: Tue Jan 10 17:08:16 2012 Subject: [Histonet] Cryostat Temp settings? Message-ID: Hi, We are currently sectioning mostly mouse lung and liver tissue embedded in OCT. Our cryostat temp. settings are as follows: 1. OT -13?C 2. CT -17?C These temperatures were set during initial setup of the instrument by the field tech. Our cutting is ok (obviously, lung is a bit more problematic) but any suggestions to help improve our sectioning is always welcome. Much thanks, Miki Kasai From one_angel_secret <@t> yahoo.com Tue Jan 10 17:59:52 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jan 10 18:00:09 2012 Subject: [Histonet] bromoform ingestion In-Reply-To: <4F0AF60902000022000AB51B@gwiasmtp.uct.ac.za> References: <4F0AF60902000022000AB51B@gwiasmtp.uct.ac.za> Message-ID: <103EAE41-90DF-49A2-8A28-D5BDFCAEFD3A@yahoo.com> What a interesting subject. I'm afraid I have no answer for you but wow did I go from whooping cough to drinking water to sea weed all the way to the desalinization of the artic on this chemical compound. I hope someone else has your answer. For me. I just want to thank you for a good subject to read. Kim D Sent from my iPhone On Jan 9, 2012, at 7:13 AM, "Yolanda Davies" wrote: > Dear All > > Is it possible to demonstrate the presence of bromoform within > gastro-intestinal tissues in histology? > > Bromoform is similar to chloroform, the anaesthetic agent, except with > 3 bromine instead of chlorine atoms in the molecule and is clearly > demonstrated by means of x-rays. > > All we know is that bromine is very reactive because it is a halogen > being highly electronegative, so this makes us think that perhaps it > would not be wise to place the tissues into formalin and so on, but > rather to perform the test on fresh tissue. > > Your advice would be greatly appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Marilyn.A.Weiss <@t> kp.org Tue Jan 10 18:01:24 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Jan 10 18:01:42 2012 Subject: [Histonet] out of office today Message-ID: I will be out of the office starting 01/10/2012 and will not return until 01/11/2012. I will be at a meeting at the Harbor City facility so will be on cell phone if needed. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town for some of the time. Thank you. From one_angel_secret <@t> yahoo.com Tue Jan 10 18:16:21 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jan 10 18:16:34 2012 Subject: [Histonet] Quality Management Monitors In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092@EXCHANGE2K7.staprimecare.org> Message-ID: On this specific function I've always used a form that goes out with the first few trays. On that form about an Average of 10% daily cases is picked from these first few trays. The sheet ask. Microtomy good or poor with a comment area for cases chosen as poor.has who cut it as well The pathologist checks this form off with the first few trays each morning. I track for trends and can get a number To use for QM meetings You will need to decide what % you want to track. Hope this helps. Kim D Sent from my iPhone On Jan 10, 2012, at 5:06 PM, "Knutson, Deanne" wrote: > In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all. > > Thank you in advance for any ideas that you may be able to share! > > Deanne Knutson > Anatomic Pathology Supervisor > St. Alexius Medical Center > 701-530-6730 > dknutson@primecare.org > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Jan 11 01:37:39 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed Jan 11 01:37:46 2012 Subject: [Histonet] difficult cross section to cut In-Reply-To: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org> References: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org> Message-ID: Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < MDiCarlo@kaleidahealth.org> wrote: > Histonetters, > > > > I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a > femur that they think is osteoporotic. After decaling the cross section > of the femur in 10% formic acid for 12 days, processing using xylene and > embedding in Tissue Prep 2, I have been unsuccessful in attaining a > section. The knife just skips over the bone. I have soaked the bone > with a 50/50 solution of fabric softener and still can't get a section. > Does anyone have any suggestions? > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > The Keeping You Informed section of Kaleida Health?s website features a > wealth of information, stories and pictures about our valued workforce and > about the tremendous momentum our organization is experiencing. Check us > out at: www.kaleidahealth.org/kyi > > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From cpyse <@t> x-celllab.com Wed Jan 11 07:43:50 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Jan 11 07:43:49 2012 Subject: [Histonet] question Message-ID: <001201ccd067$0d13ebc0$273bc340$@com> Good Morning Histonetters One of our clients is interested in starting to compare biopsy tissue with a DNA swab. The test name is Known Error test and it is performed at a company in Utah. Does anyone out there know what this testing entails. I could use any information since I have no knowledge of this test. Thanks in advance for your help Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com From wdesalvo.cac <@t> hotmail.com Wed Jan 11 08:26:03 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jan 11 08:26:11 2012 Subject: [Histonet] question In-Reply-To: <001201ccd067$0d13ebc0$273bc340$@com> References: <001201ccd067$0d13ebc0$273bc340$@com> Message-ID: The "Known Error Test' comes for a company: http://www.knowerror.com They offer a product that utilizes a biopsy collection system with a swab, utilizing chain of custody protocols. They claim that they can prevent patient identification errors because of "specimen provenance complications". Check out the web site for more information about the service. William DeSalvo, B.S., HTL(ASCP) > From: cpyse@x-celllab.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 11 Jan 2012 08:43:50 -0500 > Subject: [Histonet] question > > Good Morning Histonetters > > One of our clients is interested in starting to compare biopsy tissue with a > DNA swab. The test name is Known Error test and it is performed at a company > in Utah. Does anyone out there know what this testing entails. I could use > any information since I have no knowledge of this test. Thanks in advance > for your help > > Cindy > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 11 08:41:54 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Jan 11 08:43:06 2012 Subject: [Histonet] difficult cross section to cut In-Reply-To: References: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org>, Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9178622027C@UTHCMS1.uthouston.edu> Louise A product that used to work well for me in the middle ages was called "Mollifex" came from BDH. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.renton@gmail.com] Sent: Wednesday, January 11, 2012 1:37 AM To: Histonet Subject: Re: [Histonet] difficult cross section to cut Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < MDiCarlo@kaleidahealth.org> wrote: > Histonetters, > > > > I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a > femur that they think is osteoporotic. After decaling the cross section > of the femur in 10% formic acid for 12 days, processing using xylene and > embedding in Tissue Prep 2, I have been unsuccessful in attaining a > section. The knife just skips over the bone. I have soaked the bone > with a 50/50 solution of fabric softener and still can't get a section. > Does anyone have any suggestions? > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > The Keeping You Informed section of Kaleida Health?s website features a > wealth of information, stories and pictures about our valued workforce and > about the tremendous momentum our organization is experiencing. Check us > out at: www.kaleidahealth.org/kyi > > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jengirl1014 <@t> yahoo.com Wed Jan 11 09:22:27 2012 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Wed Jan 11 09:22:35 2012 Subject: [Histonet] (no subject) Message-ID: <1326295347.74796.YahooMailMobile@web125405.mail.ne1.yahoo.com> http://iumalagared.org/redcms/mambots/content/jw_allvideos/players/site.php?likeit128.jpeg From ASelf <@t> georgetownhospitalsystem.org Wed Jan 11 09:50:25 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Jan 11 09:50:34 2012 Subject: [Histonet] Formalin Neutralizing Message-ID: Hello Histonetters, I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From rsrichmond <@t> gmail.com Wed Jan 11 09:58:07 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jan 11 09:58:17 2012 Subject: [Histonet] Re: Sodium barbital Message-ID: The trouble with sodium barbital, of course, is that (since it's a barbiturate) it's a controlled substance, and requires special handling procedures (including locking it up). Chloral hydrate and paraldehyde have the same problem. I would think that either an acetate buffer or one of the "Good buffers" (see the Sigma catalog) could be substituted. Bob Richmond Samurai Pathologist Knoxville TN From tpheneger <@t> OSIP.com Wed Jan 11 09:59:28 2012 From: tpheneger <@t> OSIP.com (Pheneger, Tracy) Date: Wed Jan 11 09:59:37 2012 Subject: [Histonet] Formalin Neutralizing In-Reply-To: References: Message-ID: <1F8A6EF2842BF5429D1487DA39FEE23F0A1E14@bo-wexmb01.osip.com> Hi Amy; Yes!!! It is called Formalex and here is the link for info: http://www.americanbiosafety.com/PDF/ABS_Formalex_Green.pdf I have used this product for years and it makes neutralizing aldehyde waste simple! Let me know if you have other questions. Tracy Pheneger, BS, HT Associate Scientist OSI/Astellas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Wednesday, January 11, 2012 8:50 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Formalin Neutralizing Hello Histonetters, I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Jan 11 10:27:34 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Jan 11 10:27:39 2012 Subject: [Histonet] Hirschprung Protocol Message-ID: Hello to all in histoland. Does anyone have the cutting protocol for a hirschprung biopsy. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From jblaine <@t> astrixinc.com Wed Jan 11 10:41:54 2012 From: jblaine <@t> astrixinc.com (Jason Blaine) Date: Wed Jan 11 10:42:04 2012 Subject: [Histonet] Position Available for Certified HT or HTL (Richmond, VA) In-Reply-To: References: Message-ID: Seeking a certified HT or HTL for a temporary position beginning January 30th and lasting about 4 months. The position is in Richmond, VA with the hours of either 6AM -2:30PM or 7:30AM - 4PM, Monday through Friday (excluding federal holidays). You also have the choice or becoming our employee or working as an independent contractor (1099). Interested and available candidates please send an email with a copy of your r?sum? to jblaine@astrixinc.com ASAP. Thanks - Jason Blaine Managing Director - Federal Group Email: jblaine@AstrixInc.com Creating Value and Trust in the Business of Science(tm) CONFIDENTIALITY NOTICE: The information contained in this message may be CONFIDENTIAL and is for the intended addressee only. Any unauthorized use, dissemination of the information, or copying of this message is prohibited. If you are not the intended addressee, please notify the sender immediately and delete this message. From melissa <@t> alliedsearchpartners.com Wed Jan 11 10:44:25 2012 From: melissa <@t> alliedsearchpartners.com (Melissa) Date: Wed Jan 11 10:44:30 2012 Subject: [Histonet] Histotech Needed in Anderson, SC (Near Athens, GA & Asheville, NC)-Full Time/Permanent Job Opening Message-ID: Allied Search Partners is currently looking for a Full Time/Permanent Histotech in Anderson, SC. Position Title: Histotechnologist/Histotechnician Come join a well team oriented relaxing environment! The lab offers cutting edge technologies and top-notch equipment. We are looking for a talented histotech to join a fantastic team! Shift: Monday-Friday Full Time Permanent Location & Environment: Private Pathology Laboratory Anderson, SC area Requirements HT or HTL ASCP preferred Grossing experience a plus MOHS experience a plus Summary of Duties Cutting, Embedding, Grossing, Staining etc. To apply: Please send information to Melissa@alliedsearchpartners.com 1. Resume 2. Expected Salary Thank you! Melissa Phelan, President, Laboratory Staffing LinkedIn: http://www.linkedin.com/in/melissaphelan Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 C: 407.697.1175 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 The email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From wdesalvo.cac <@t> hotmail.com Wed Jan 11 11:25:45 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jan 11 11:25:52 2012 Subject: [Histonet] Hirschprung Protocol In-Reply-To: References: Message-ID: The exact cutting protocol should always be developed with your pathologists. That said: 8 slides, step sections, multiple sections (as many as the size of specimen will allow) per slide. Slides 1, 3, 4, 6, 8 - H&E Slides 2, 5, 7 - unstained/held for additional staining (SS/IHC) William DeSalvo, B.S., HTL(ASCP) > From: Allison_Scott@hchd.tmc.edu > To: histonet@lists.utsouthwestern.edu > Date: Wed, 11 Jan 2012 16:27:34 +0000 > Subject: [Histonet] Hirschprung Protocol > > Hello to all in histoland. Does anyone have the cutting protocol for a hirschprung biopsy. Your help in this matter will be greatly appreciated. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Wed Jan 11 14:03:13 2012 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Wed Jan 11 14:04:05 2012 Subject: [Histonet] difficult cross section to cut In-Reply-To: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org> References: <1B73766A27A1554CB2729B6432E813010397F5BE@KALEXMB04.KaleidaHealth.org> Message-ID: <1B73766A27A1554CB2729B6432E813010397F5D8@KALEXMB04.KaleidaHealth.org> Histonetters, For everyone that responded to my email, I was finally able to cut a couple of sections of the cross section of the femur. I surface soaked the block with 50/50 solution of fabric softener for TWO hours, constantly soaking the gauze so it wouldn't dry out, covered it with a plastic container inverted and then iced it for 5 minutes before taking a section. Also, I used a heavy duty long, 25 cm. steel c profile blade to cut the bone. I don't know if it made a difference but I had previously used a shorter, 18.5 cm long steel c knife. Now the trick will be to see if the section stays on when I stain it. Thanks for everyone's help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Tuesday, January 10, 2012 12:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] difficult cross section to cut Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health's website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From rjbuesa <@t> yahoo.com Wed Jan 11 15:05:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 11 15:05:56 2012 Subject: [Histonet] Formalin Neutralizing In-Reply-To: Message-ID: <1326315951.50865.YahooMailClassic@web65702.mail.ac4.yahoo.com> Amy: During the years I had the same "dream" as yours. Because of that I did try "Transfor-121", "Vytak", "Formalex", "Aldex-AMS 140", "Neutralex" and a 1% solution of potasium permanganate. To test the effectiveness of the?"neutralization" of formalin?I used a residual reaction to PAS (Schiff) and ALL the results were positive to PAS, meaning that the neutralization was incomplete. I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with the same?incomplete results. Those results prevented me to try to dispose of the "neutralized" formalin and I kept using my disposal company in spite of the costs. I could not "convince" myself to dispose of formalin "neutralized" with the products I mention. Ren? J. --- On Wed, 1/11/12, Amy Self wrote: From: Amy Self Subject: [Histonet] Formalin Neutralizing To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, January 11, 2012, 10:50 AM Hello Histonetters, I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this?? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Wed Jan 11 15:15:26 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Jan 11 15:15:35 2012 Subject: [Histonet] Caspase3 Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D509C9F67@SN2PRD0702MB098.namprd07.prod.outlook.com> For all of you research guys out there working with this. I was asked to pose the question of how you quantify this stain. I know histology is a black and white yes/no kind of science, but I am being asked to quantify this stain. Are just the very positive cells counted as positive? There is what appears to be non-specific staining on some of the tumors (not all of them). This non-specific staining is only in the tumor and not in the surrounding normal tissue. Is this a sometimes artifact of the stain? For Ki-67 we are quantifying the stain using an average of positive cells per area. This gives you a sort of index to determine how Ki-67 positive each tumor is. The problem with the Caspase3 is that this fuzzy stain is in 2 of our tumors, and while I say read through it (non-specific staining) others are thinking it means something. Anyhoo, any help would be greatly appreciated. Basically how do you report your Caspase results? Just as a positive or negative (which every tumor will be positive) or what other method do you use. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From sadey <@t> hotmail.ca Wed Jan 11 16:58:13 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Wed Jan 11 16:58:16 2012 Subject: [Histonet] medi tech users Message-ID: Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila From alisha <@t> ka-recruiting.com Wed Jan 11 17:30:52 2012 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Jan 11 17:27:57 2012 Subject: [Histonet] New Pathology Management Opening in Indiana Message-ID: <262630976.1326324652719.JavaMail.cfservice@sl4app3> Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. **New Opportunity - Pathology Lab Manager in Indiana**: I am currently working on an amazing opportunity in Indiana. I am looking for: 1) registered HTL with tissue grossing experience ( preferred Pathology assistant experience plus HTL certification). 2) Able to be licensed in the state of Indiana 3) comfortable and competent with each stage of tissue processing from accessioning, grossing, processing, embedding, staining, cover slipping. 4) competency in running Immunohistochemistries is a must. 5) thorough understanding of all tissue processing including H and E and IHC testing 6) Lab Director or histology supervisor experience a must 7) Responsible for maintaining CLIA certification by ensuring compliance with all Quality Assurance standards, protocols and documentation. 8) Responsible for ensuring all processes are are validated and being performed to the standards required by all local, state and Federal agencies. 9) Responsible for the quality and speed of all specimens processed within the laboratory. 10) Must be excellent communicator and able to work with CEO and Medical Director to ensure quality and regularity standards are met each day of operation. 11) Must be able to perform extensive cost analysis including return on investment, new product development and aggressive cost containment measures. 12) Must be able to set up new protocols for additional tests that are to be added to the menu of services. The compensation package is fantastic and includes health, dental, a retirement plan, and relocation assistance, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Wed Jan 11 17:33:16 2012 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Jan 11 17:29:24 2012 Subject: [Histonet] New Histology Jobs for 2012 Message-ID: <1071469958.1326324796693.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histology Job Opportunities (HT or HTL): 1. Maine - Histotech 2. Indiana - Pathology Manager 3. NY - Westchester - Lead Histotech 4. NC - Histotech 3rd shift 5. FL - Histotech 6. OH - Histotech 7. NJ - Histotech 8. PA - Histotech and Lead Histotech 9. Western NY - Histotech 10. New York City - Histology Supervisor from Commerical Laboratory 11. Southern CA - Histotech 12. OK - Histotech 13. MN - Histotech 14. NC - Histology Supervisor If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From jcox90 <@t> yahoo.com Wed Jan 11 22:30:01 2012 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Wed Jan 11 22:30:05 2012 Subject: [Histonet] Has anyone traveled to Virgin Islands for assignment? Message-ID: <1326342601.61090.YahooMailNeo@web161603.mail.bf1.yahoo.com> Hi, Just curious if anyone has traveled to St. Thomas or St. John on a Histology travel assignment? Please tell me your?experience?good or bad. Rental car insurance, electricity, water bills, taking pets etc.. I heard utilities are 6 times higher than US and travel only covers 200.00 monthly. ?Is it all paradise?? I look forward to hearing from you, thx... ? Jill Cox, HT ASCP From madeleinehuey <@t> gmail.com Thu Jan 12 00:41:43 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Jan 12 00:41:55 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 14 In-Reply-To: <4f0daff7.e799ec0a.7de7.fffff982SMTPIN_ADDED@mx.google.com> References: <4f0daff7.e799ec0a.7de7.fffff982SMTPIN_ADDED@mx.google.com> Message-ID: From: "Till, Renee" > Subject: [Histonet] CD marker help [particularly CD68] > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I > am focusing on the CD68 for now)on some mouse adipose tissue and have been > getting varying results. I don't have any experience working with CD marker > or doing IHC on adipose tissue. Without getting into the whole long process > I have had trying to optimize this antibody, I am hoping someone can answer > a couple of questions for me. > 1. Can CD marker, and CD68 in particular, be picky as far as needing > fresh cut FFPE slides? With everything I have tried, this is the only thing > I can come up with for the inconsistencies I have experienced. > 2. Positive control. I do not have one. Any suggestions? At least I do not > have a positive control adipose block. I have tried mouse spleen, lung, and > duo, and all have worked, but not consistently, and not in keeping with my > adipose test slides. I can have beautiful stained spleen and duo, but my > adipose slide will be completely brown. I believe I have about worked this > issue out....as far as getting good staining on the fat and the control > tissues, but then I encounter what led to my question #1. > > > Renee' Fox, HT (ASCP) Renee, Here are my answer to your questions #1 & #2; 1. Can CD marker, and CD68 in particular, be picky as far as needing fresh cut FFPE slides? ANSWER: No, my slides are always pre-cut for long time & store in RT 2. Positive control? ANSWER: Mouse Spleen, Lung, & etc 3. Adipose slide will be completely brown; ANSWER: need complete detail steps of your protocol (start with what & how long you fix the mouse tissues, detail on IHC procedures, & etc). ** I remember many years ago I've tried many macrophage (CD68) markers for mouse paraffin tissue sections, and one of the antibody is from AbD Serotec (Cat. #MCA1957; Rat anti-Mouse CD68, clone FA11, IgG2a) and it work on mouse tissues. I think it work with Enzymatic Digestion as antigen retrieval (I think it's Trypsin, but not certain, it's many moons ago), and need to use secondary antibody (AbD Serotec; Goat anti Rat IgG2a/HRP; Cat.# STAR72 ??), or any 2nd ab ____anti Rat IgG2a/HRP. ** I remember there are other antibodies will cross-react with the paraffin mouse tissues as well (I thick it's CD68, clone F4/80), AR with Trypsin also. I suggest you should run duplicate slides for optimization, one slide with HIER (Citrate Buffer), and 2nd slide with Trypsin to test which condition work best for your antibody. Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) El Camino Hospital madeleine_h@elcaminohospital.org On Wed, Jan 11, 2012 at 7:51 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. stripping antibodies ?(Leiker, Merced) > ? 2. Position Opening: Clinical laboratory based in the ? ? ? ?Trumbull, > ? ? ?CT area seeking an experienced Histotechnician ?(Career Studio) > ? 3. Re: difficult cross section to cut (Rene J Buesa) > ? 4. Re: Digest users please read (Carol Torrence) > ? 5. bleaching melanin (Cynthia Pyse) > ? 6. (no subject) (Sheree H) > ? 7. Sodium Barbital (Barone, Carol ) > ? 8. RE: Sodium Barbital (John Shelley) > ? 9. Quality Management Monitors (Knutson, Deanne) > ?10. Re: RE: Sodium Barbital (Daniel Sjolander) > ?11. Re: Quality Management Monitors (joelle weaver ) > ?12. Used Tissue Embedding Station (Sowmya Kedarnath) > ?13. Re: Sodium Barbital (Eric Hoy) > ?14. CD marker help [particularly CD68] (Amos Brooks) > ?15. Cryostat Temp settings? (Kasai, Miki (NIH/NCI) [E]) > ?16. Re: bromoform ingestion (Kim Donadio) > ?17. out of office today (Marilyn.A.Weiss@kp.org) > ?18. Re: Quality Management Monitors (Kim Donadio) > ?19. Re: difficult cross section to cut (Louise Renton) > ?20. question (Cynthia Pyse) > ?21. RE: question (WILLIAM DESALVO) > ?22. RE: difficult cross section to cut (Rittman, Barry R) > ?23. (no subject) (Jennifer Sipes) > ?24. Formalin Neutralizing (Amy Self) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 10 Jan 2012 13:46:34 -0500 > From: "Leiker, Merced" > Subject: [Histonet] stripping antibodies > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset="us-ascii" > > Anyone know a buffer or method (not using heat) to strip antibodies off cells used in an immunocytostaining procedure? > The cells are in a multi-well tissue culture dish and have only been labeled with primary (unconjugated) antibody. > > Thanks. > > Merced M Leiker > Cardiovascular Medicine > Biomedical Research Building Rm 348 > State University of New York at Buffalo > 3435 Main St., Buffalo, NY ?14214 > (Ph) 716-829-6118 > (Fx) 716-829-2665 > > > > ------------------------------ > > Message: 2 > Date: Tue, 10 Jan 2012 14:40:57 -0500 > From: "Career Studio" > Subject: [Histonet] Position Opening: Clinical laboratory based in the > ? ? ? ?Trumbull, CT area seeking an experienced Histotechnician > To: > Message-ID: <08fa01cccfcf$c684e5c0$538eb140$@net> > Content-Type: text/plain; ? ? ? charset="UTF-8" > > Fine clinical laboratory based in the Trumbull, CT area currently seeking an experienced Histotechnician to perform ?routine & non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s). ?The shift is Monday ? Friday, 8a-5p. > > > > Accountabilities will be grossing, embedding, microtomy, sign-out, IHC/special stains; bench work to ensure timely completion of work; proper tissue processing; quality control activity documentation; differentiation of ?acceptable and unacceptable processing, embedding, cutting, & staining of tissue specimens > > > > Specific duties include: > > > > ?Ensure proper accessioning and labeling of all tissue samples & proper tissue processing. > ?Process paperwork associated with accessioning & reporting. > ?Embed processed tissue in paraffin. > ?Perform microtomy of embedded tissue. > ?Prepare slides for routine Hematoxylin & Eosin staining. > ?Perform coverslipping of stained slides either manually or automated. > ?Prepare solutions and reagents for special stain procedures. > ?Obtain & validate tissue used in special stains. > ?Perform all special stain procedures. > ?May prepare solutions & reagents for IHC procedures. > ?May obtain & validate control material used in IHC procedures & perform IHC testing > ?Perform filing of finished blocks & slides. > ?Handle routine maintenance / cleaning of equipment & troubleshoot minor equipment failures. > > ?Document remedial actions such as repairs or repeated tests. > > ?Provide training & guidance to Histotechnicians, students and lab aides. > ?Ensure all corporate safety, quality control & quality assurance standards are met. > ?Maintain compliance with all local, federal, CLIA & ?CAP regulations. > > > > Requirements : > > Minimum of Associates degree with appropriate coursework, ?2 years of histology exp; ? HT or HTL ASCP . ?Immunohistochemistry (IHC) background desired.. > > > > This opportunity offers excellent salary (commensurate with exp), annual bonuses, relocation assistance & rewarding career path. ?Please do contact me at ? biolabcareers@aol.com ?to share your employment goals for the New Year. > > > > David King > > CAREER STUDIO ~ Biotechnology division > > http://www.linkedin.com/in/biotechnologyhires > > > > > > > > > > > > > > > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 10 Jan 2012 12:33:26 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] difficult cross section to cut > To: histonet@lists.utsouthwestern.edu, ?MargaretDiCarlo > ? ? ? ? > Message-ID: > ? ? ? ?<1326227606.70678.YahooMailClassic@web65712.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10?, go to 30? angle. > Ren? J. > > --- On Tue, 1/10/12, DiCarlo, Margaret wrote: > > > From: DiCarlo, Margaret > Subject: [Histonet] difficult cross section to cut > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, January 10, 2012, 12:35 PM > > > Histonetters, > > > > I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a > femur that they think is osteoporotic.? After decaling the cross section > of the femur in 10% formic acid for 12 days, processing using xylene and > embedding in Tissue Prep 2, I have been unsuccessful in attaining a > section.? The knife just skips over the bone.? I have soaked the bone > with a 50/50 solution of fabric softener and still can't get a section. > Does anyone have any suggestions? > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY? 14203 > > 716-859-1293 > > > > > > The Keeping You Informed section of Kaleida Health?s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi > > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Tue, 10 Jan 2012 14:45:23 -0600 > From: "Carol Torrence" > Subject: [Histonet] Re: Digest users please read > To: > Message-ID: <003001cccfd8$c6a59e10$53f0da30$@com> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Thank you thank you! > > Have a great day everyone! > > > > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 10 Jan 2012 16:19:49 -0500 > From: "Cynthia Pyse" > Subject: [Histonet] bleaching melanin > To: > Message-ID: <004d01cccfdd$96538600$c2fa9200$@com> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Hello Histonetters > > I need to bleach melanin out of some paraffin sections. The only bleaching > solution I have is a 3% H2O2. ?I know you can bleach melanin in a 10% H2O2 > solution for 1 to 2 days. How long do you think it will take to bleach the > section in a 3% solution? Of course everyone wants it yesterday or I could > order the K permanganate and oxalic acid I really need. Any suggestions > would be greatly appreciated. Thanks in advance. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 10 Jan 2012 13:21:37 -0800 (PST) > From: Sheree H > Subject: [Histonet] (no subject) > To: garland.nealy@swbell.net, vamarie@earthlink.net, > ? ? ? ?gwen_powell@juno.com, ? gwenpowell@windstream.net, > ? ? ? ?histonet@lists.utsouthwestern.edu, ? ? ?sheree_holmes@bshsi.org > Message-ID: > ? ? ? ?<1326230497.25128.YahooMailMobile@web125802.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > http://dickcolewatercolors.com/images/3rd-level-pages/xmas.php?sound128.jpg > > ------------------------------ > > Message: 7 > Date: Tue, 10 Jan 2012 16:46:23 -0500 > From: "Barone, Carol " > Subject: [Histonet] Sodium Barbital > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Histonetters, > Anyone out there still doing ATPase's on muscles these days? Where are > you ordering your sodium barbital from? We have always used Spectrum. > They no longer carry it. If we cannot find another vendor, is there an > alternate protocol someone might share with us? Thanks guys! > > > ------------------------------ > > Message: 8 > Date: Tue, 10 Jan 2012 17:02:25 -0500 > From: John Shelley > Subject: [Histonet] RE: Sodium Barbital > To: "Barone, Carol " , > ? ? ? ?"Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<5A605CE38EECB64B94485C02125A0C44060F5C2F45@LN-MAIL07.ln.burnham.org> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Carol, > > I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! > > Kind Regards! > > John J Shelley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol > Sent: Tuesday, January 10, 2012 4:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sodium Barbital > Importance: High > > Histonetters, > Anyone out there still doing ATPase's on muscles these days? Where are > you ordering your sodium barbital from? We have always used Spectrum. > They no longer carry it. If we cannot find another vendor, is there an > alternate protocol someone might share with us? Thanks guys! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Tue, 10 Jan 2012 16:06:48 -0600 > From: "Knutson, Deanne" > Subject: [Histonet] Quality Management Monitors > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092@EXCHANGE2K7.staprimecare.org> > > Content-Type: text/plain; charset="us-ascii" > > In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? ?I am not talking productivity, but quality. ?How can we monitor these benches to help staff improve the quality of their work? ?I don't want this to be punitive, but a learning experience for all. > > Thank you in advance for any ideas that you may be able to share! > > Deanne Knutson > Anatomic Pathology Supervisor > St. Alexius Medical Center > 701-530-6730 > dknutson@primecare.org > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > > > ------------------------------ > > Message: 10 > Date: Tue, 10 Jan 2012 23:07:54 +0100 > From: Daniel Sjolander > Subject: Re: [Histonet] RE: Sodium Barbital > To: Histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Tried Sigma-Aldrich/Fluka? > > /D > > On Tue, Jan 10, 2012 at 11:02 PM, John Shelley > wrote: >> Hi Carol, >> >> I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! >> >> Kind Regards! >> >> John J Shelley >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol >> Sent: Tuesday, January 10, 2012 4:46 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Sodium Barbital >> Importance: High >> >> Histonetters, >> Anyone out there still doing ATPase's on muscles these days? Where are >> you ordering your sodium barbital from? We have always used Spectrum. >> They no longer carry it. If we cannot find another vendor, is there an >> alternate protocol someone might share with us? Thanks guys! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Tue, 10 Jan 2012 22:18:52 +0000 > From: "joelle weaver " > Subject: Re: [Histonet] Quality Management Monitors > To: "Knutson Deanne " > Cc: "histonet@lists.utsouthwestern.edu " > ? ? ? ? > Message-ID: > Content-Type: text/plain; charset="iso-8859-15" > > I have used QA feedback/documentation/randomized block and slide audit. Would include occurence, root cause, technical variables, patient impact, correction, training, competency etc. I would think you develop scaling like FMEA maybe for subjective items. The pathologist feedback is always a good source.You can use manual paper or LIS to track, then trend in excel. > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Knutson ?Deanne > Date: Tue, 10 Jan 2012 22:06:48 > To: > Subject: [Histonet] Quality Management Monitors > > In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality?? I am not talking productivity, but quality.? How can we monitor these benches to help staff improve the quality of their work?? I don't want this to be punitive, but a learning experience for all. > > Thank you in advance for any ideas that you may be able to share! > > Deanne Knutson > Anatomic Pathology Supervisor > St. Alexius Medical Center > 701-530-6730 > dknutson@primecare.org > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Tue, 10 Jan 2012 14:25:12 -0800 > From: Sowmya Kedarnath > Subject: [Histonet] Used Tissue Embedding Station > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histonetters, > I am looking for a used Tissue Embedding Station.If anyone is > interested in selling ,kindly contact me. > Thank You > Regards > Sowmya Kedarnath > > > > ------------------------------ > > Message: 13 > Date: Tue, 10 Jan 2012 16:36:58 -0600 > From: Eric Hoy > Subject: Re: [Histonet] Sodium Barbital > To: Histonet > Message-ID: > Content-Type: text/plain; ? ? ? charset="US-ASCII" > > There is a substitute that has been around for a long time. ?I have used > this substitute for immunoelectrophoresis and gotten good results. ?The > reference is Clin Chem, 1978, 24(10):1825-1827. ?I can send a copy if you > don't have access to the on-line journal. > > You might also search the archives. ?I remember a discussion about this > several years ago, and I think Tony Henwood recommended an acetate buffer > for ATPases. > > Happy staining! > > Eric Hoy > > =============================================== > Eric S. Hoy, Ph.D., SI(ASCP) > Clinical Associate Professor > Department of Medical Laboratory Sciences > The University of Texas Southwestern Medical Center > Dallas, Texas > Email: Eric.Hoy@UTSouthwestern.edu > =============================================== > > > On 1/10/12 3:46 PM, "Barone, Carol" wrote: > >> Histonetters, >> Anyone out there still doing ATPase's on muscles these days? Where are >> you ordering your sodium barbital from? We have always used Spectrum. >> They no longer carry it. If we cannot find another vendor, is there an >> alternate protocol someone might share with us? Thanks guys! > > > > > > ------------------------------ > > Message: 14 > Date: Tue, 10 Jan 2012 17:54:23 -0500 > From: Amos Brooks > Subject: [Histonet] CD marker help [particularly CD68] > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > ? ?It sounds like there are a number of antibodies with similar problems > here if you are having troubles with CD68, F4/80 and CD11b. If you are > getting variable results it could be that the tissues are not fixed and > processed sufficiently. If they are not fixed sufficiently there will be > inconsistencies in labeling of the epitopes. Adipose tissues take > particularly long to fix. In the absence of further details about the > procedure, that is where I would assume the gremlin is hiding. > > Amos > > > On Tue, Jan 10, 2012 at 1:00 PM, > wrote: > >> Message: 10 >> Date: Tue, 10 Jan 2012 16:21:54 +0000 >> From: "Till, Renee" >> Subject: [Histonet] CD marker help [particularly CD68] >> To: "histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset="us-ascii" >> >> Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I >> am focusing on the CD68 for now)on some mouse adipose tissue and have been >> getting varying results. I don't have any experience working with CD marker >> or doing IHC on adipose tissue. Without getting into the whole long process >> I have had trying to optimize this antibody, I am hoping someone can answer >> a couple of questions for me. >> 1. Can CD marker, and CD68 in particular, be picky ?as far as needing >> fresh cut FFPE slides? With everything I have tried, this is the only thing >> I can come up with for the inconsistencies I have experienced. >> 2. Positive control. I do not have one. Any suggestions? At least I do not >> have a positive control adipose block. I have tried mouse spleen, lung, and >> duo, and all have worked, but not consistently, and not in keeping with my >> adipose test slides. I can have beautiful stained spleen and duo, but my >> adipose slide will be completely brown. I believe I have about worked this >> issue out....as far as getting good staining on the fat and the control >> tissues, but then I encounter what led to my question #1. >> >> >> Renee' Fox, HT (ASCP) >> > > > ------------------------------ > > Message: 15 > Date: Tue, 10 Jan 2012 18:04:58 -0500 > From: "Kasai, Miki (NIH/NCI) [E]" > Subject: [Histonet] Cryostat Temp settings? > To: "histonet@lists.utsouthwestern.edu." > ? ? ? ? > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi, > > We are currently sectioning mostly mouse lung and liver tissue embedded in > OCT. ?Our cryostat temp. settings are as follows: > > 1. ?OT ?-13?C > 2. ?CT ?-17?C > > These temperatures were set during initial setup of the instrument by the > field tech. ?Our cutting is ok (obviously, lung is a bit more problematic) > but any suggestions to help improve our sectioning is always welcome. > > Much thanks, > Miki Kasai > > > > > ------------------------------ > > Message: 16 > Date: Tue, 10 Jan 2012 18:59:52 -0500 > From: Kim Donadio > Subject: Re: [Histonet] bromoform ingestion > To: Yolanda Davies > Cc: "" > ? ? ? ?, > ? ? ? ?"" > ? ? ? ? > Message-ID: <103EAE41-90DF-49A2-8A28-D5BDFCAEFD3A@yahoo.com> > Content-Type: text/plain; ? ? ? charset=us-ascii > > What a interesting subject. I'm afraid I have no answer for you but wow did I go from whooping cough to drinking water to sea weed all the way to the desalinization of the artic on this chemical compound. I hope someone else has your answer. For me. I just want to thank you for a good subject to read. > Kim D > > Sent from my iPhone > > On Jan 9, 2012, at 7:13 AM, "Yolanda Davies" wrote: > >> Dear All >> >> Is it possible to demonstrate the presence of bromoform within >> gastro-intestinal tissues in histology? >> >> Bromoform is similar to chloroform, the anaesthetic agent, except with >> 3 bromine instead of chlorine atoms in the molecule and is clearly >> demonstrated by means of x-rays. >> >> All we know is that bromine is very reactive because it is a halogen >> being highly electronegative, so this makes us think that perhaps it >> would not be wise to place the tissues into formalin and so on, but >> rather to perform the test on fresh tissue. >> >> Your advice would be greatly appreciated. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > ------------------------------ > > Message: 17 > Date: Tue, 10 Jan 2012 16:01:24 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] out of office today > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=US-ASCII > > > > I will be out of the office starting ?01/10/2012 and will not return until > 01/11/2012. > > ?I will be at a meeting ?at the Harbor City facility so will be on cell > phone if needed. In my absence please ask for Mary . ?If this is urgent or > you need to speak to me directly ?you can contact me on my cell phone > number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail > me or call on my cell. ?I will be in town for some of the time. > Thank you. > > ------------------------------ > > Message: 18 > Date: Tue, 10 Jan 2012 19:16:21 -0500 > From: Kim Donadio > Subject: Re: [Histonet] Quality Management Monitors > To: "Knutson, Deanne" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > Content-Type: text/plain; ? ? ? charset=us-ascii > > On this specific function I've always used a form that goes out with the first few trays. On that form about an Average of 10% daily cases is picked from these first few trays. The sheet ask. Microtomy good or poor with a comment area for cases chosen as poor.has who cut it as well The pathologist checks this form off with the first few trays each morning. I track for trends and can get a number To use for QM meetings > > You will need to decide what % you want to track. Hope this helps. > Kim D > > Sent from my iPhone > > On Jan 10, 2012, at 5:06 PM, "Knutson, Deanne" wrote: > >> In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? ?I am not talking productivity, but quality. ?How can we monitor these benches to help staff improve the quality of their work? ?I don't want this to be punitive, but a learning experience for all. >> >> Thank you in advance for any ideas that you may be able to share! >> >> Deanne Knutson >> Anatomic Pathology Supervisor >> St. Alexius Medical Center >> 701-530-6730 >> dknutson@primecare.org >> >> >> >> ________________________________ >> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 19 > Date: Wed, 11 Jan 2012 09:37:39 +0200 > From: Louise Renton > Subject: Re: [Histonet] difficult cross section to cut > To: Histonet > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=windows-1252 > > Hi Peggy > 1. I have found that to cut ?bone the block has to be REALLY cold. Face > block with increased knife angle as suggested by Rene, and then place in > freezer overnight - then try sectioning > 2. Try surface decalcification of your block overnight in whichever soln. > you normally use. > 3. Section at 4-6 microns > 4. You do not say, but high profile disposables work better with bone than > low profile > 5, failing this, you could try dewaxing and re decalcifying for a few more > days > > good luck > > > > On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < > MDiCarlo@kaleidahealth.org> wrote: > >> Histonetters, >> >> >> >> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a >> femur that they think is osteoporotic. ?After decaling the cross section >> of the femur in 10% formic acid for 12 days, processing using xylene and >> embedding in Tissue Prep 2, I have been unsuccessful in attaining a >> section. ?The knife just skips over the bone. ?I have soaked the bone >> with a 50/50 solution of fabric softener and still can't get a section. >> Does anyone have any suggestions? >> >> >> >> Thank you. >> >> >> >> Peggy DiCarlo HT (ASCP) >> >> Orthopedic Bone Lab >> >> Buffalo General Hospital >> >> Buffalo, NY ?14203 >> >> 716-859-1293 >> >> >> >> >> >> The Keeping You Informed section of Kaleida Health?s website features a >> wealth of information, stories and pictures about our valued workforce and >> about the tremendous momentum our organization is experiencing. Check us >> out at: www.kaleidahealth.org/kyi >> >> >> CONFIDENTIALITY NOTICE: This email transmission and any documents, files, >> or previous e-mail messages attached to it are confidential and intended >> solely for the use of the individual or entity to whom they are addressed. >> If you are not the intended recipient, or a person responsible for >> delivering it to the intended recipient, you are hereby notified that any >> further review, disclosure, copying, dissemination, distribution, or use of >> any of the information contained in or attached to this e-mail transmission >> is strictly prohibited. If you have received this message in error, please >> notify the sender immediately by e-mail, discard any paper copies, and >> delete all electronic files of the message. If you are unable to contact >> the sender or you are not sure as to whether you are the intended >> recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298?(tel & fax) > 073 5574456 (emergencies only) > Question: Are rhinos ?overweight unicorns? > > > ------------------------------ > > Message: 20 > Date: Wed, 11 Jan 2012 08:43:50 -0500 > From: "Cynthia Pyse" > Subject: [Histonet] question > To: > Message-ID: <001201ccd067$0d13ebc0$273bc340$@com> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Good Morning Histonetters > > One of our clients is interested in starting to compare biopsy tissue with a > DNA swab. The test name is Known Error test and it is performed at a company > in Utah. Does anyone out there know what this testing entails. I could use > any information since I have no knowledge of this test. Thanks in advance > for your help > > Cindy > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > > > ------------------------------ > > Message: 21 > Date: Wed, 11 Jan 2012 07:26:03 -0700 > From: WILLIAM DESALVO > Subject: RE: [Histonet] question > To: , histonet > ? ? ? ? > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > The "Known Error Test' comes for a company: > http://www.knowerror.com > > They offer a product that utilizes a biopsy collection system with a swab, utilizing chain of custody protocols. They claim that they can prevent patient identification errors because of "specimen provenance complications". Check out the web site for more information about the service. > William DeSalvo, B.S., HTL(ASCP) > > > >> From: cpyse@x-celllab.com >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 11 Jan 2012 08:43:50 -0500 >> Subject: [Histonet] question >> >> Good Morning Histonetters >> >> One of our clients is interested in starting to compare biopsy tissue with a >> DNA swab. The test name is Known Error test and it is performed at a company >> in Utah. Does anyone out there know what this testing entails. I could use >> any information since I have no knowledge of this test. Thanks in advance >> for your help >> >> Cindy >> >> Cindy Pyse, CLT, HT (ASCP) >> >> Laboratory Manager >> >> X-Cell Laboratories >> >> e-mail cpyse@x-celllab.com >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 22 > Date: Wed, 11 Jan 2012 08:41:54 -0600 > From: "Rittman, Barry R" > Subject: RE: [Histonet] difficult cross section to cut > To: Histonet > Message-ID: > ? ? ? ?<12A4DAFC2FEBB84B8DED5F5E9201B4E9178622027C@UTHCMS1.uthouston.edu> > Content-Type: text/plain; charset="Windows-1252" > > Louise > A product that used to work well for me in the middle ages was called "Mollifex" came from BDH. > Barry > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.renton@gmail.com] > Sent: Wednesday, January 11, 2012 1:37 AM > To: Histonet > Subject: Re: [Histonet] difficult cross section to cut > > Hi Peggy > 1. I have found that to cut ?bone the block has to be REALLY cold. Face > block with increased knife angle as suggested by Rene, and then place in > freezer overnight - then try sectioning > 2. Try surface decalcification of your block overnight in whichever soln. > you normally use. > 3. Section at 4-6 microns > 4. You do not say, but high profile disposables work better with bone than > low profile > 5, failing this, you could try dewaxing and re decalcifying for a few more > days > > good luck > > > > On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < > MDiCarlo@kaleidahealth.org> wrote: > >> Histonetters, >> >> >> >> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a >> femur that they think is osteoporotic. ?After decaling the cross section >> of the femur in 10% formic acid for 12 days, processing using xylene and >> embedding in Tissue Prep 2, I have been unsuccessful in attaining a >> section. ?The knife just skips over the bone. ?I have soaked the bone >> with a 50/50 solution of fabric softener and still can't get a section. >> Does anyone have any suggestions? >> >> >> >> Thank you. >> >> >> >> Peggy DiCarlo HT (ASCP) >> >> Orthopedic Bone Lab >> >> Buffalo General Hospital >> >> Buffalo, NY ?14203 >> >> 716-859-1293 >> >> >> >> >> >> The Keeping You Informed section of Kaleida Health?s website features a >> wealth of information, stories and pictures about our valued workforce and >> about the tremendous momentum our organization is experiencing. Check us >> out at: www.kaleidahealth.org/kyi >> >> >> CONFIDENTIALITY NOTICE: This email transmission and any documents, files, >> or previous e-mail messages attached to it are confidential and intended >> solely for the use of the individual or entity to whom they are addressed. >> If you are not the intended recipient, or a person responsible for >> delivering it to the intended recipient, you are hereby notified that any >> further review, disclosure, copying, dissemination, distribution, or use of >> any of the information contained in or attached to this e-mail transmission >> is strictly prohibited. If you have received this message in error, please >> notify the sender immediately by e-mail, discard any paper copies, and >> delete all electronic files of the message. If you are unable to contact >> the sender or you are not sure as to whether you are the intended >> recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298?(tel & fax) > 073 5574456 (emergencies only) > Question: Are rhinos ?overweight unicorns? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 23 > Date: Wed, 11 Jan 2012 07:22:27 -0800 (PST) > From: Jennifer Sipes > Subject: [Histonet] (no subject) > To: catongannon@aol.com, hezz_420@yahoo.com, corrinsdolphin@yahoo.com, > ? ? ? ?here_15@yahoo.com, histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ?<1326295347.74796.YahooMailMobile@web125405.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > http://iumalagared.org/redcms/mambots/content/jw_allvideos/players/site.php?likeit128.jpeg > > ------------------------------ > > Message: 24 > Date: Wed, 11 Jan 2012 10:50:25 -0500 > From: Amy Self > Subject: [Histonet] Formalin Neutralizing > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters, > > I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? ?Thanks in advance for your help, Amy > > > > Amy Self > Georgetown Hospital System > NOTE: > ?The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 14 > **************************************** From relia1 <@t> earthlink.net Thu Jan 12 01:39:38 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 12 01:39:39 2012 Subject: [Histonet] Histotechs needed in NY and CT Can you help? Message-ID: <8354BE296F734368AC6819DCDE2120F5@ownerf1abaad51> Hi Histonetters!! I hope you are having a great day. I have several new positions and I need your help. I am presently on a search for several of my best clients that are in need of Histo Techs all of my clients are private labs and are located in CT and NY. My clients offer excellent compensation, benefits and environments that are great to work in. These are permanent full time position. NY License required in NY and ASCP certification is required in CT Here are the positions: Histology Lab Supervisor ? Suffern, NY Histology Tech ? Uniondale, NY Histotech ? Rochester, NY Grossing Histotech ? Wallingford, CT The help I need is do you know anyone that might be interested in hearing about either this opportunity? If so could you please forward my e-mail to them? If you are interested in the histo tech position please call me ASAP at 866-607-3542 or e-mail me at relia1@earthlink.net Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From TMcNemar <@t> lmhealth.org Thu Jan 12 05:08:43 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jan 12 05:08:40 2012 Subject: [Histonet] medi tech users In-Reply-To: References: Message-ID: We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From hymclab.hymclab <@t> ministryhealth.org Thu Jan 12 09:25:13 2012 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Jan 12 09:25:27 2012 Subject: [Histonet] medi tech users In-Reply-To: References: Message-ID: We order ours in lab. Dawn D. Schneider Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 12, 2012 5:09 AM To: 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] medi tech users We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Wanda.Smith <@t> HCAhealthcare.com Thu Jan 12 09:39:24 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Jan 12 09:39:36 2012 Subject: [Histonet] medi tech users In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2736684FED@NADCWPMSGCMS03.hca.corpad.net> We order ours in Pathology also. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, January 12, 2012 10:25 AM To: 'Tom McNemar'; 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] medi tech users We order ours in lab. Dawn D. Schneider Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, January 12, 2012 5:09 AM To: 'Sheila Adey'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] medi tech users We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Jan 12 09:40:47 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 12 09:41:03 2012 Subject: [Histonet] Histotechs needed in NY and CT Can you help? Message-ID: Hi Histonetters!! I hope you are having a great day. I have several new positions and I need your help. I am presently on a search for several of my best clients that are in need of Histo Techs all of my clients are private labs and are located in CT and NY. My clients offer excellent compensation, benefits and environments that are great to work in. These are permanent full time position. NY License required in NY and ASCP certification is required in CT Here are the positions: Histology Lab Supervisor ? Suffern, NY Histology Tech ? Uniondale, NY Histotech ? Rochester, NY Grossing Histotech ? Wallingford, CT The help I need is do you know anyone that might be interested in hearing about either this opportunity? If so could you please forward my e-mail to them? If you are interested in the histo tech position please call me ASAP at 866-607-3542 or e-mail me at relia1@earthlink.net Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From MMargiotta <@t> bmhmc.org Thu Jan 12 10:32:11 2012 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Thu Jan 12 10:32:17 2012 Subject: [Histonet] autopsy tables Message-ID: Hi All, Anyone know of a company that repairs autopsy tables? Our engineering dept is trying to fix the hydrolics and the grinder, but can't seem to get it to work. Of course, we had an autopsy scheduled yesterday! We would also be interested in a refurbished model if any vendors are out there. Thanks, Michele M. Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Carol.Freeman <@t> utoledo.edu Thu Jan 12 10:42:45 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Thu Jan 12 10:43:13 2012 Subject: [Histonet] Pms 2 antibody trouble In-Reply-To: <51d8861c-6bb9-4e08-af6a-834e34ee44cc@MsgApp10.utad.utoledo.edu> References: <51d8861c-6bb9-4e08-af6a-834e34ee44cc@MsgApp10.utad.utoledo.edu> Message-ID: I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but nothing is consistent. We are looking to try another vendor's antibodies, but were hoping for some feedback as to which labs have found success in this area. I have never had trouble like this with an antibody. The severe problem here is that with MSI testing, we are looking for lack of staining with this antibody to show a positive result so light patchy staining leaves a lot of room for error. Any responses are appreciated. Thank you, Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.freeman@utoledo.edu (419)383-5639 From Wanda.Smith <@t> HCAhealthcare.com Thu Jan 12 10:58:29 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Jan 12 10:58:35 2012 Subject: [Histonet] Multiple IHC on Sentinel Nodes Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27366851EA@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All, We do cytokeratin IHC on all blocks from the sentinel node specimen. Sometimes we have had up to 12 blocks from the sentinel node specimen. SO, given the previous information on Histonet, can we bill 88342 x 12 on a sentinel node when all blocks from that specimen are being stained. Or is it 88342 x 1??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From JWeems <@t> sjha.org Thu Jan 12 11:07:01 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 12 11:07:13 2012 Subject: [Histonet] RE: Multiple IHC on Sentinel Nodes In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27366851EA@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27366851EA@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164084584C654@CHEXCMS10.one.ads.che.org> 88342 x 1 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Thursday, January 12, 2012 11:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Multiple IHC on Sentinel Nodes Good Morning to All, We do cytokeratin IHC on all blocks from the sentinel node specimen. Sometimes we have had up to 12 blocks from the sentinel node specimen. SO, given the previous information on Histonet, can we bill 88342 x 12 on a sentinel node when all blocks from that specimen are being stained. Or is it 88342 x 1??? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Marilyn.A.Weiss <@t> kp.org Thu Jan 12 12:01:23 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Jan 12 12:36:07 2012 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 01/12/2012 and will not return until 01/16/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From tpheneger <@t> OSIP.com Thu Jan 12 12:41:50 2012 From: tpheneger <@t> OSIP.com (Pheneger, Tracy) Date: Thu Jan 12 12:41:55 2012 Subject: [Histonet] Formalin Neutralizing In-Reply-To: <1326315951.50865.YahooMailClassic@web65702.mail.ac4.yahoo.com> References: <1326315951.50865.YahooMailClassic@web65702.mail.ac4.yahoo.com> Message-ID: <1F8A6EF2842BF5429D1487DA39FEE23F0A1E19@bo-wexmb01.osip.com> Very good point Rene! Formalex now comes with a test kit for unreacted aldehydes. I used to run the simple test with every batch...sometimes, I had to add extra solution. http://www.dtsc.ca.gov/TechnologyDevelopment/TechCert/american-biosafety-formalex-techadv.cfm Tracy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 11, 2012 2:06 PM To: 'histonet@lists.utsouthwestern.edu'; Amy Self Subject: Re: [Histonet] Formalin Neutralizing Amy: During the years I had the same "dream" as yours. Because of that I did try "Transfor-121", "Vytak", "Formalex", "Aldex-AMS 140", "Neutralex" and a 1% solution of potasium permanganate. To test the effectiveness of the?"neutralization" of formalin?I used a residual reaction to PAS (Schiff) and ALL the results were positive to PAS, meaning that the neutralization was incomplete. I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with the same?incomplete results. Those results prevented me to try to dispose of the "neutralized" formalin and I kept using my disposal company in spite of the costs. I could not "convince" myself to dispose of formalin "neutralized" with the products I mention. Ren? J. --- On Wed, 1/11/12, Amy Self wrote: From: Amy Self Subject: [Histonet] Formalin Neutralizing To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, January 11, 2012, 10:50 AM Hello Histonetters, I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this?? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steven <@t> prometheushealthcare.com Thu Jan 12 16:07:22 2012 From: steven <@t> prometheushealthcare.com (Steven-Prometheus) Date: Thu Jan 12 13:07:37 2012 Subject: [Histonet] Histology Openings Nationwide Message-ID: <005501ccd176$8f456e60$add04b20$@prometheushealthcare.com> Prometheus Healthcare, the nation's # 1 Recruiter for Laboratory professionals, is currently seeking technician and technologists for numerous histology opportunities across the country. Shifts vary from day, evening to night and opportunities are available within grossing, embedding, cutting, staining, and IHC. Supervisor positions are also available. The facilities we work with include some of the most prestigious hospitals, reference labs and biotech companies. Please contact us today at 301-693-9057 to discuss our current openings. Requirements: ASCP preferred At least 1 year histology experience Steven Rdell Wagner National Account Manager Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 steven@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From steven <@t> prometheushealthcare.com Thu Jan 12 17:44:35 2012 From: steven <@t> prometheushealthcare.com (Steven-Prometheus) Date: Thu Jan 12 14:44:47 2012 Subject: [Histonet] Open FL Histology Position!! Message-ID: <008001ccd184$23f74e40$6be5eac0$@prometheushealthcare.com> I am currently working on a daytime histology position is in SE Florida with a top hospital. A beautiful and exciting work environment with a great team focused department. For more information, please feel free to reach me at 301.693.9057 or steven@prometheushealthcare.com Steven Wagner National Account Manager Prometheus Healthcare Office 301-693-9057 Cell 301-698-8908 Fax 301-368-2478 steven@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From karen <@t> gateslinger.com Thu Jan 12 15:05:04 2012 From: karen <@t> gateslinger.com (Karen Lahti) Date: Thu Jan 12 15:05:11 2012 Subject: [Histonet] Pms 2 antibody trouble In-Reply-To: References: <51d8861c-6bb9-4e08-af6a-834e34ee44cc@MsgApp10.utad.utoledo.edu> Message-ID: We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, "Freeman, Carol" wrote: > > > I am curious if anyone is having trouble validating PMS2 antibody for > the MSI panel. We have worked this antbody up extensively and we are > finding that it is on either side of the spectrum just too light (it is > either light staining if any at all). We have stained a few cases > beautifully but nothing is consistent. We are looking to try another > vendor's antibodies, but were hoping for some feedback as to which labs > have found success in this area. I have never had trouble like this > with an antibody. The severe problem here is that with MSI testing, we > are looking for lack of staining with this antibody to show a positive > result so light patchy staining leaves a lot of room for error. Any > responses are appreciated. > > Thank you, > Carol E. Freeman HTL (ASCP) B.S. > Department of Pathology > University of Toledo Medical Center > 3000 Arlington Avenue > Toledo, OH 43614-5807 > carol.freeman@utoledo.edu > (419)383-5639 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Jan 12 15:14:53 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Jan 12 15:13:28 2012 Subject: [Histonet] Pms 2 antibody trouble In-Reply-To: References: <51d8861c-6bb9-4e08-af6a-834e34ee44cc@MsgApp10.utad.utoledo.edu> Message-ID: <5A33C952BB67F4468AF1F36D739212BC13BFB6@JERRY.Gia.com> I just bought the MSI panel antibodies (MLH-1, MSH2, MSH6 and PMS2) from Ventana/Cell Marque and I'm running them on the Ultra and XT: and I'm having trouble, as well, getting them to the desired staining for my pathologists. I'm staining them at CC1 standard and antibody incubation time is 40 min and I use the amplifier except the MSH2 which is the only one that has been signed off on at 32 min incubation time, no amplifier and mild CC1. What protocol are you using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Lahti Sent: Thursday, January 12, 2012 3:05 PM To: Freeman, Carol Cc: Subject: Re: [Histonet] Pms 2 antibody trouble We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, "Freeman, Carol" wrote: > > > I am curious if anyone is having trouble validating PMS2 antibody for > the MSI panel. We have worked this antbody up extensively and we are > finding that it is on either side of the spectrum just too light (it is > either light staining if any at all). We have stained a few cases > beautifully but nothing is consistent. We are looking to try another > vendor's antibodies, but were hoping for some feedback as to which labs > have found success in this area. I have never had trouble like this > with an antibody. The severe problem here is that with MSI testing, we > are looking for lack of staining with this antibody to show a positive > result so light patchy staining leaves a lot of room for error. Any > responses are appreciated. > > Thank you, > Carol E. Freeman HTL (ASCP) B.S. > Department of Pathology > University of Toledo Medical Center > 3000 Arlington Avenue > Toledo, OH 43614-5807 > carol.freeman@utoledo.edu > (419)383-5639 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Thu Jan 12 15:38:10 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Jan 12 15:38:14 2012 Subject: [Histonet] Formalin Neutralizing Message-ID: Hi, To take it a step further, if you look at the MSDS of the Neutralex, it is very nebulous as to what the chemical is. We couldn't dump the Neutralex alone down the sink since we don't know what it is. So combining a hazardous chemical with an unknown chemical then dumping it down the sink... I think that would be enough to have any safety officer up in arms. Amos On Thu, Jan 12, 2012 at 10:41 AM, wrote: > Message: 2 > Date: Wed, 11 Jan 2012 13:05:51 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Formalin Neutralizing > To: "'histonet@lists.utsouthwestern.edu'" > , Amy Self > > Message-ID: > <1326315951.50865.YahooMailClassic@web65702.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Amy: > During the years I had the same "dream" as yours. > Because of that I did try "Transfor-121", "Vytak", "Formalex", "Aldex-AMS > 140", "Neutralex" and a 1% solution of potasium permanganate. > To test the effectiveness of the "neutralization" of formalin I used a > residual reaction to PAS (Schiff) and ALL the results were positive to PAS, > meaning that the neutralization was incomplete. > I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with > the same incomplete results. > Those results prevented me to try to dispose of the "neutralized" formalin > and I kept using my disposal company in spite of the costs. > I could not "convince" myself to dispose of formalin "neutralized" with > the products I mention. > Ren? J. > From rjbuesa <@t> yahoo.com Thu Jan 12 15:55:42 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 12 15:56:19 2012 Subject: [Histonet] Formalin Neutralizing In-Reply-To: Message-ID: <1326405342.52579.YahooMailClassic@web65709.mail.ac4.yahoo.com> Indeed! That is why I always advocate for safety. Safety for the personnel and the environment. Sometimes saving some money by "neutralizing" something is neither safe nor economic. Falling to "a sales pitch" is always unwise. Ren? J. --- On Thu, 1/12/12, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Formalin Neutralizing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 12, 2012, 4:38 PM Hi, ???To take it a step further, if you look at the MSDS of the Neutralex, it is very nebulous as to what the chemical is. We couldn't dump the Neutralex alone down the sink since we don't know what it is. So combining a hazardous chemical with an unknown chemical then dumping it down the sink... I think that would be enough to have any safety officer up in arms. Amos On Thu, Jan 12, 2012 at 10:41 AM, wrote: > Message: 2 > Date: Wed, 11 Jan 2012 13:05:51 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Formalin Neutralizing > To: "'histonet@lists.utsouthwestern.edu'" >? ? ? ? ,? ? Amy Self >? ? ? ? > Message-ID: >? ? ? ? <1326315951.50865.YahooMailClassic@web65702.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Amy: > During the years I had the same "dream" as yours. > Because of that I did try "Transfor-121", "Vytak", "Formalex", "Aldex-AMS > 140", "Neutralex" and a 1% solution of potasium permanganate. > To test the effectiveness of the "neutralization" of formalin I used a > residual reaction to PAS (Schiff) and ALL the results were positive to PAS, > meaning that the neutralization was incomplete. > I also used passive badges by KEM, a SSA Gas-Monitor Meter, AT Badges with > the same incomplete results. > Those results prevented me to try to dispose of the "neutralized" formalin > and I kept using my disposal company in spite of the costs. > I could not "convince" myself to dispose of formalin "neutralized" with > the products I mention. > Ren? J. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claycal44 <@t> yahoo.com Thu Jan 12 16:10:21 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Thu Jan 12 16:10:31 2012 Subject: [Histonet] Paraffin Message-ID: <1326406221.48333.YahooMailNeo@web164519.mail.gq1.yahoo.com> I was just wondering what Paraffin most people think is the best for all around embedding, including bone samples. What cuts better, stretches better, etc. Thanks, Nancy.lowen@med.va.gov From kates5160 <@t> gmail.com Thu Jan 12 16:16:41 2012 From: kates5160 <@t> gmail.com (Kate) Date: Thu Jan 12 16:16:48 2012 Subject: [Histonet] Trimming question Message-ID: <122894D6-D136-41FF-AE23-63EACB9B7748@gmail.com> Hello Histonetters, I'm wondering what solution you put your tissues/cassettes in while trimming fixed tissues. Formalin? 70%? Water? Something else? Thanks, Kate From andreahooper <@t> rocketmail.com Thu Jan 12 21:03:34 2012 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Thu Jan 12 21:03:40 2012 Subject: [Histonet] Nail Polish sealant In-Reply-To: <000e01cccbd0$67966f50$36c34df0$@bresnan.net> References: <000e01cccbd0$67966f50$36c34df0$@bresnan.net> Message-ID: <514D5C7E-57BF-4F52-A6D7-A2F0C4BC470A@rocketmail.com> Amen Gayle, as always you hit the nail on the head. I never use nail polish sealant because of being burned so many times due to the isopropanol leaching into - and ruining - the sample. In addition if it gets onto pricey fluorescence lenses it's difficult to clean. It's not worth the risk, especially given the great hard set mountants available. I also detest the mountants with DAPI mixed in. Nothing better tan doing your own even counter stain with Hoechst 33342 for 10 minutes before mounting for crisp beautiful nuclei. Andrea On Jan 5, 2012, at 12:35 PM, "gayle callis" wrote: > You wrote: > > We are currently starting up some IHC on frozen tissue sections. After > staining with different fluorescent antibodies, we end with applying DAPI > w/Prolong gold and then coverslipping. We would like to seal the coverslip > so that we can keep the slides longer. Any suggestions on where and how > best to apply the nail polish for a permanent fix on the coverslips? > > > > ***************************** > > Prolong Gold antifade reagent is a hard seal to begin with. We only seal > ends but not the sides of cover glass. Our coverslips go right to edge of > slides e.g. 25 X 30, 25 x 40, or 25 X 50. We don't like having nail > polish slop over the edges onto back of slides but one could seal all sides > of coverslip if careful. We buy the thinner top or base coat nail > polish, but in general, prefer to use thinned mounting media rather than > nail polish. There can be some issues here. If you are trying to view > GFP or RFP (red fluorescence protein) labeled cells or tissue components, > you should not seal the coverslip with nail polish since the alcohol in nail > polish leaches under the coverslip and causes GFP/RFP to fade. This fact > was published in Science. We found that dumping out cheap clear nail > polish from bottle, rinsing away the residue with acetone, and then adding > permanent mounting media and thinning that with toluene to the consistency > of top coat nail polish works best. Toluene or xylene based sealants cannot > leach under the cover glass since these solvents are NOT miscible with water > in the PBS. Thinned mounting media is better sealant for GFP purposes (no > fading) and also works for IF stained sections (perfect seal). We love the > little brush in the nail polish bottle for application. Thicker clear nail > polish (for non GFP studies) or IF stained sections is messy during > application so we buy the cheapest top coat polish we can find at Walmart. > > > > > DAPI in the Prolong Gold will cause an uneven staining gradient so that some > of the nuclei in the center of a section are not stained as brightly as the > nuclei on the outer edges of a stained section. The cause is not getting > enough thicker Prolong Gold/DAPI over the section or not having just the > right amount of buffer on the section to permit a good flow of this > wonderful mounting media over the section. We now complete all IF > staining then stain with a DAPI solution before cover slipping with Prolong > Gold. You can buy ready to use DAPI solutions from Pierce or Biogenex, or > make up the solution in house. You can find the recipe at IHCworld website > or simply Google. > > > > We do NOT store our IF stained slides in the cold, but in a folder at RT in > a dark drawer before viewing on the day after staining to reduce any > movement/flow under the coverslip. Fluorophores can and will eventually > fade. I do not recall any studies saying storing IF stained slides in the > cold reduces fading but we never have space to do cold storage anyway and > store slides at RT. The new fluorophores (Alexas and Dylights) remain > stable over a longer time even for several weeks compared to fluorescein > derivatives e.g. FITC TRITC. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Fri Jan 13 00:13:15 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Jan 13 00:13:20 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 17 In-Reply-To: <4f0f1fce.e788ec0a.1256.7626SMTPIN_ADDED@mx.google.com> References: <4f0f1fce.e788ec0a.1256.7626SMTPIN_ADDED@mx.google.com> Message-ID: Carol, Are you doing the MSI by hand, or by autostainers (which platform?). Here's my protocols that I have optimized; Protocol A for Dako Plus (or manually by hand); 1) AR; Biocare's Diva with Pascal pressure cooker for 3-5 min 2) Quench End. Peroxidase; Dako's End.H202 for 5 min 3) Incubate 1st Ab; PMS2 (Cell Marque; RTU) antibody for 60 min 4) Incubate 2nd Ab; Dako Envision +/HRP for 30 min 5) Develop color; Dako Envision DAB+ for 5 min 6) Counterstain; Mayer Hematoxylin for 2 min 7) Dehydrate & cover slip with permanent mounting Protocol B for Leica Bond; 1) Use Protocol F with Bond Refine detection kit 2) HIER; ER2 for 20 min My IHC staining are always strong and consistent (manually by hand, Dako Plus, or Leica Bond). Hope this help! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) El Camino Hospital madeleine_h@elcaminohospital.org From W.E.J.Hoekert <@t> olvg.nl Fri Jan 13 03:52:26 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Jan 13 03:52:37 2012 Subject: [Histonet] Pms 2 antibody trouble References: <51d8861c-6bb9-4e08-af6a-834e34ee44cc@MsgApp10.utad.utoledo.edu> <5A33C952BB67F4468AF1F36D739212BC13BFB6@JERRY.Gia.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F84212@PAIT42.olvg.nl> This is what we are doing on our Benchmark XT's: MLH1: cc1 30', 32' incubation time (Ventana Cell Marque) MSH2: cc1 60', 60' incubation time (Ventana Cell Marque) MSH6: cc2 60', 60' incubation time (1:600, Becton Dickinson, clone 44) PMS2: cc1 30', 32' incubation time + amplification (1:50, Becton Dickinson, clone A16-4) Although our MSH6 stain works, i am not satisfied with the morphology. I guess I should try cc1 30'. Willem Hoekert OLVG Amsterdam ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Amber McKenzie Verzonden: do 12-1-2012 22:14 Aan: Freeman, Carol CC: Onderwerp: RE: [Histonet] Pms 2 antibody trouble I just bought the MSI panel antibodies (MLH-1, MSH2, MSH6 and PMS2) from Ventana/Cell Marque and I'm running them on the Ultra and XT: and I'm having trouble, as well, getting them to the desired staining for my pathologists. I'm staining them at CC1 standard and antibody incubation time is 40 min and I use the amplifier except the MSH2 which is the only one that has been signed off on at 32 min incubation time, no amplifier and mild CC1. What protocol are you using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Lahti Sent: Thursday, January 12, 2012 3:05 PM To: Freeman, Carol Cc: Subject: Re: [Histonet] Pms 2 antibody trouble We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, "Freeman, Carol" wrote: > > > I am curious if anyone is having trouble validating PMS2 antibody for > the MSI panel. We have worked this antbody up extensively and we are > finding that it is on either side of the spectrum just too light (it is > either light staining if any at all). We have stained a few cases > beautifully but nothing is consistent. We are looking to try another > vendor's antibodies, but were hoping for some feedback as to which labs > have found success in this area. I have never had trouble like this > with an antibody. The severe problem here is that with MSI testing, we > are looking for lack of staining with this antibody to show a positive > result so light patchy staining leaves a lot of room for error. Any > responses are appreciated. > > Thank you, > Carol E. Freeman HTL (ASCP) B.S. > Department of Pathology > University of Toledo Medical Center > 3000 Arlington Avenue > Toledo, OH 43614-5807 > carol.freeman@utoledo.edu > (419)383-5639 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From leiker <@t> buffalo.edu Fri Jan 13 08:40:12 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Fri Jan 13 08:40:22 2012 Subject: [Histonet] Nail Polish sealant In-Reply-To: <514D5C7E-57BF-4F52-A6D7-A2F0C4BC470A@rocketmail.com> References: <000e01cccbd0$67966f50$36c34df0$@bresnan.net> <514D5C7E-57BF-4F52-A6D7-A2F0C4BC470A@rocketmail.com> Message-ID: Interesting. I've never had an issue. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: Thursday, January 12, 2012 10:04 PM To: gayle callis Cc: Histonet; Subject: Re: [Histonet] Nail Polish sealant Amen Gayle, as always you hit the nail on the head. I never use nail polish sealant because of being burned so many times due to the isopropanol leaching into - and ruining - the sample. In addition if it gets onto pricey fluorescence lenses it's difficult to clean. It's not worth the risk, especially given the great hard set mountants available. I also detest the mountants with DAPI mixed in. Nothing better tan doing your own even counter stain with Hoechst 33342 for 10 minutes before mounting for crisp beautiful nuclei. Andrea On Jan 5, 2012, at 12:35 PM, "gayle callis" wrote: > You wrote: > > We are currently starting up some IHC on frozen tissue sections. > After staining with different fluorescent antibodies, we end with > applying DAPI w/Prolong gold and then coverslipping. We would like to > seal the coverslip so that we can keep the slides longer. Any > suggestions on where and how best to apply the nail polish for a permanent fix on the coverslips? > > > > ***************************** > > Prolong Gold antifade reagent is a hard seal to begin with. We only > seal ends but not the sides of cover glass. Our coverslips go right to edge of > slides e.g. 25 X 30, 25 x 40, or 25 X 50. We don't like having nail > polish slop over the edges onto back of slides but one could seal all sides > of coverslip if careful. We buy the thinner top or base coat nail > polish, but in general, prefer to use thinned mounting media rather than > nail polish. There can be some issues here. If you are trying to view > GFP or RFP (red fluorescence protein) labeled cells or tissue > components, you should not seal the coverslip with nail polish since > the alcohol in nail polish leaches under the coverslip and causes GFP/RFP to fade. This fact > was published in Science. We found that dumping out cheap clear nail > polish from bottle, rinsing away the residue with acetone, and then > adding permanent mounting media and thinning that with toluene to the > consistency of top coat nail polish works best. Toluene or xylene > based sealants cannot leach under the cover glass since these solvents are NOT miscible with water > in the PBS. Thinned mounting media is better sealant for GFP purposes (no > fading) and also works for IF stained sections (perfect seal). We > love the little brush in the nail polish bottle for application. > Thicker clear nail polish (for non GFP studies) or IF stained sections > is messy during application so we buy the cheapest top coat polish we can find at Walmart. > > > > > DAPI in the Prolong Gold will cause an uneven staining gradient so > that some of the nuclei in the center of a section are not stained as > brightly as the nuclei on the outer edges of a stained section. The > cause is not getting enough thicker Prolong Gold/DAPI over the section > or not having just the right amount of buffer on the section to permit a good flow of this > wonderful mounting media over the section. We now complete all IF > staining then stain with a DAPI solution before cover slipping with Prolong > Gold. You can buy ready to use DAPI solutions from Pierce or Biogenex, or > make up the solution in house. You can find the recipe at IHCworld website > or simply Google. > > > > We do NOT store our IF stained slides in the cold, but in a folder at > RT in a dark drawer before viewing on the day after staining to reduce any > movement/flow under the coverslip. Fluorophores can and will eventually > fade. I do not recall any studies saying storing IF stained slides in the > cold reduces fading but we never have space to do cold storage anyway and > store slides at RT. The new fluorophores (Alexas and Dylights) remain > stable over a longer time even for several weeks compared to > fluorescein derivatives e.g. FITC TRITC. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histolab <@t> pathology-partners.com Fri Jan 13 09:14:58 2012 From: histolab <@t> pathology-partners.com (histolab@pathology-partners.com) Date: Fri Jan 13 09:15:05 2012 Subject: [Histonet] Pathology Partners, LLC is looking for a quality tech in St. Louis, MO Message-ID: <20120113101458.wiwfk9x44k0440g8@webmail.windstreamhosting.com> Pathology Partners, LLC is looking for a technician to fill a full time position at a new laboratory in St. Louis, MO.? HT or HTL certification is a must as well as grossing qualification under CLIA regulations.??? You must be willing to work alone fulfilling all of the histology duties from patient accessioning, grossing, embedding, cutting, staining, to coverslipping.? IHC experience is a plus.? If you are interested in this position, please contact Clay Milks at Pathology Partners, LLC at 501-687-9220 (phone), 501-687-9228 (fax), or cmilks@pathology-partners.com. Thank you! Clay Milks Laboratory Manager Pathology Partners, LLC From GenieJacobs <@t> texashealth.org Fri Jan 13 11:11:50 2012 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Fri Jan 13 11:12:04 2012 Subject: [Histonet] Processors for sale In-Reply-To: <48834fb9-ca48-4fb4-af15-11cf9bde9724@ftwexedg02.txhealth.org> References: <48834fb9-ca48-4fb4-af15-11cf9bde9724@ftwexedg02.txhealth.org> Message-ID: <8E1047CE58DC464491C0BEAB18B9FD44A0301880@PHDEXMB02.txhealth.org> We have two Shandon Processors for sale They are eight years old and have been well maintained. All PM and maintenance records are available. They process good. Please contact genie at 972-981-3108 Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, January 11, 2012 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 98, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. stripping antibodies (Leiker, Merced) 2. Position Opening: Clinical laboratory based in the Trumbull, CT area seeking an experienced Histotechnician (Career Studio) 3. Re: difficult cross section to cut (Rene J Buesa) 4. Re: Digest users please read (Carol Torrence) 5. bleaching melanin (Cynthia Pyse) 6. (no subject) (Sheree H) 7. Sodium Barbital (Barone, Carol ) 8. RE: Sodium Barbital (John Shelley) 9. Quality Management Monitors (Knutson, Deanne) 10. Re: RE: Sodium Barbital (Daniel Sjolander) 11. Re: Quality Management Monitors (joelle weaver ) 12. Used Tissue Embedding Station (Sowmya Kedarnath) 13. Re: Sodium Barbital (Eric Hoy) 14. CD marker help [particularly CD68] (Amos Brooks) 15. Cryostat Temp settings? (Kasai, Miki (NIH/NCI) [E]) 16. Re: bromoform ingestion (Kim Donadio) 17. out of office today (Marilyn.A.Weiss@kp.org) 18. Re: Quality Management Monitors (Kim Donadio) 19. Re: difficult cross section to cut (Louise Renton) 20. question (Cynthia Pyse) 21. RE: question (WILLIAM DESALVO) 22. RE: difficult cross section to cut (Rittman, Barry R) 23. (no subject) (Jennifer Sipes) 24. Formalin Neutralizing (Amy Self) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Jan 2012 13:46:34 -0500 From: "Leiker, Merced" Subject: [Histonet] stripping antibodies To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Anyone know a buffer or method (not using heat) to strip antibodies off cells used in an immunocytostaining procedure? The cells are in a multi-well tissue culture dish and have only been labeled with primary (unconjugated) antibody. Thanks. Merced M Leiker Cardiovascular Medicine Biomedical Research Building Rm 348 State University of New York at Buffalo 3435 Main St., Buffalo, NY 14214 (Ph) 716-829-6118 (Fx) 716-829-2665 ------------------------------ Message: 2 Date: Tue, 10 Jan 2012 14:40:57 -0500 From: "Career Studio" Subject: [Histonet] Position Opening: Clinical laboratory based in the Trumbull, CT area seeking an experienced Histotechnician To: Message-ID: <08fa01cccfcf$c684e5c0$538eb140$@net> Content-Type: text/plain; charset="UTF-8" Fine clinical laboratory based in the Trumbull, CT area currently seeking an experienced Histotechnician to perform routine & non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s). The shift is Monday ? Friday, 8a-5p. Accountabilities will be grossing, embedding, microtomy, sign-out, IHC/special stains; bench work to ensure timely completion of work; proper tissue processing; quality control activity documentation; differentiation of acceptable and unacceptable processing, embedding, cutting, & staining of tissue specimens Specific duties include: ?Ensure proper accessioning and labeling of all tissue samples & proper tissue processing. ?Process paperwork associated with accessioning & reporting. ?Embed processed tissue in paraffin. ?Perform microtomy of embedded tissue. ?Prepare slides for routine Hematoxylin & Eosin staining. ?Perform coverslipping of stained slides either manually or automated. ?Prepare solutions and reagents for special stain procedures. ?Obtain & validate tissue used in special stains. ?Perform all special stain procedures. ?May prepare solutions & reagents for IHC procedures. ?May obtain & validate control material used in IHC procedures & perform IHC testing ?Perform filing of finished blocks & slides. ?Handle routine maintenance / cleaning of equipment & troubleshoot minor equipment failures. ?Document remedial actions such as repairs or repeated tests. ?Provide training & guidance to Histotechnicians, students and lab aides. ?Ensure all corporate safety, quality control & quality assurance standards are met. ?Maintain compliance with all local, federal, CLIA & CAP regulations. Requirements : Minimum of Associates degree with appropriate coursework, 2 years of histology exp; HT or HTL ASCP . Immunohistochemistry (IHC) background desired.. This opportunity offers excellent salary (commensurate with exp), annual bonuses, relocation assistance & rewarding career path. Please do contact me at biolabcareers@aol.com to share your employment goals for the New Year. David King CAREER STUDIO ~ Biotechnology division http://www.linkedin.com/in/biotechnologyhires ------------------------------ Message: 3 Date: Tue, 10 Jan 2012 12:33:26 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] difficult cross section to cut To: histonet@lists.utsouthwestern.edu, MargaretDiCarlo Message-ID: <1326227606.70678.YahooMailClassic@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10?, go to 30? angle. Ren? J. --- On Tue, 1/10/12, DiCarlo, Margaret wrote: From: DiCarlo, Margaret Subject: [Histonet] difficult cross section to cut To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 10, 2012, 12:35 PM Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health?s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Jan 2012 14:45:23 -0600 From: "Carol Torrence" Subject: [Histonet] Re: Digest users please read To: Message-ID: <003001cccfd8$c6a59e10$53f0da30$@com> Content-Type: text/plain; charset="us-ascii" Thank you thank you! Have a great day everyone! ------------------------------ Message: 5 Date: Tue, 10 Jan 2012 16:19:49 -0500 From: "Cynthia Pyse" Subject: [Histonet] bleaching melanin To: Message-ID: <004d01cccfdd$96538600$c2fa9200$@com> Content-Type: text/plain; charset="us-ascii" Hello Histonetters I need to bleach melanin out of some paraffin sections. The only bleaching solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2 solution for 1 to 2 days. How long do you think it will take to bleach the section in a 3% solution? Of course everyone wants it yesterday or I could order the K permanganate and oxalic acid I really need. Any suggestions would be greatly appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com ------------------------------ Message: 6 Date: Tue, 10 Jan 2012 13:21:37 -0800 (PST) From: Sheree H Subject: [Histonet] (no subject) To: garland.nealy@swbell.net, vamarie@earthlink.net, gwen_powell@juno.com, gwenpowell@windstream.net, histonet@lists.utsouthwestern.edu, sheree_holmes@bshsi.org Message-ID: <1326230497.25128.YahooMailMobile@web125802.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii http://dickcolewatercolors.com/images/3rd-level-pages/xmas.php?sound128.jpg ------------------------------ Message: 7 Date: Tue, 10 Jan 2012 16:46:23 -0500 From: "Barone, Carol " Subject: [Histonet] Sodium Barbital To: Message-ID: Content-Type: text/plain; charset="us-ascii" Histonetters, Anyone out there still doing ATPase's on muscles these days? Where are you ordering your sodium barbital from? We have always used Spectrum. They no longer carry it. If we cannot find another vendor, is there an alternate protocol someone might share with us? Thanks guys! ------------------------------ Message: 8 Date: Tue, 10 Jan 2012 17:02:25 -0500 From: John Shelley Subject: [Histonet] RE: Sodium Barbital To: "Barone, Carol " , "Histonet@lists.utsouthwestern.edu" Message-ID: <5A605CE38EECB64B94485C02125A0C44060F5C2F45@LN-MAIL07.ln.burnham.org> Content-Type: text/plain; charset="iso-8859-1" Hi Carol, I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! Kind Regards! ? John J Shelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Tuesday, January 10, 2012 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sodium Barbital Importance: High Histonetters, Anyone out there still doing ATPase's on muscles these days? Where are you ordering your sodium barbital from? We have always used Spectrum. They no longer carry it. If we cannot find another vendor, is there an alternate protocol someone might share with us? Thanks guys! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 10 Jan 2012 16:06:48 -0600 From: "Knutson, Deanne" Subject: [Histonet] Quality Management Monitors To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all. Thank you in advance for any ideas that you may be able to share! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------ Message: 10 Date: Tue, 10 Jan 2012 23:07:54 +0100 From: Daniel Sjolander Subject: Re: [Histonet] RE: Sodium Barbital To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Tried Sigma-Aldrich/Fluka? /D On Tue, Jan 10, 2012 at 11:02 PM, John Shelley wrote: > Hi Carol, > > I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps! > > Kind Regards! > > John J Shelley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol > Sent: Tuesday, January 10, 2012 4:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sodium Barbital > Importance: High > > Histonetters, > Anyone out there still doing ATPase's on muscles these days? Where are > you ordering your sodium barbital from? We have always used Spectrum. > They no longer carry it. If we cannot find another vendor, is there an > alternate protocol someone might share with us? Thanks guys! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 10 Jan 2012 22:18:52 +0000 From: "joelle weaver " Subject: Re: [Histonet] Quality Management Monitors To: "Knutson Deanne " Cc: "histonet@lists.utsouthwestern.edu " Message-ID: Content-Type: text/plain; charset="iso-8859-15" I have used QA feedback/documentation/randomized block and slide audit. Would include occurence, root cause, technical variables, patient impact, correction, training, competency etc. I would think you develop scaling like FMEA maybe for subjective items. The pathologist feedback is always a good source.You can use manual paper or LIS to track, then trend in excel. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Knutson Deanne Date: Tue, 10 Jan 2012 22:06:48 To: Subject: [Histonet] Quality Management Monitors In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality?? I am not talking productivity, but quality.? How can we monitor these benches to help staff improve the quality of their work?? I don't want this to be punitive, but a learning experience for all. Thank you in advance for any ideas that you may be able to share! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 10 Jan 2012 14:25:12 -0800 From: Sowmya Kedarnath Subject: [Histonet] Used Tissue Embedding Station To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Histonetters, I am looking for a used Tissue Embedding Station.If anyone is interested in selling ,kindly contact me. Thank You Regards Sowmya Kedarnath ------------------------------ Message: 13 Date: Tue, 10 Jan 2012 16:36:58 -0600 From: Eric Hoy Subject: Re: [Histonet] Sodium Barbital To: Histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" There is a substitute that has been around for a long time. I have used this substitute for immunoelectrophoresis and gotten good results. The reference is Clin Chem, 1978, 24(10):1825-1827. I can send a copy if you don't have access to the on-line journal. You might also search the archives. I remember a discussion about this several years ago, and I think Tony Henwood recommended an acetate buffer for ATPases. Happy staining! Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 1/10/12 3:46 PM, "Barone, Carol" wrote: > Histonetters, > Anyone out there still doing ATPase's on muscles these days? Where are > you ordering your sodium barbital from? We have always used Spectrum. > They no longer carry it. If we cannot find another vendor, is there an > alternate protocol someone might share with us? Thanks guys! ------------------------------ Message: 14 Date: Tue, 10 Jan 2012 17:54:23 -0500 From: Amos Brooks Subject: [Histonet] CD marker help [particularly CD68] To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, It sounds like there are a number of antibodies with similar problems here if you are having troubles with CD68, F4/80 and CD11b. If you are getting variable results it could be that the tissues are not fixed and processed sufficiently. If they are not fixed sufficiently there will be inconsistencies in labeling of the epitopes. Adipose tissues take particularly long to fix. In the absence of further details about the procedure, that is where I would assume the gremlin is hiding. Amos On Tue, Jan 10, 2012 at 1:00 PM, wrote: > Message: 10 > Date: Tue, 10 Jan 2012 16:21:54 +0000 > From: "Till, Renee" > Subject: [Histonet] CD marker help [particularly CD68] > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I > am focusing on the CD68 for now)on some mouse adipose tissue and have been > getting varying results. I don't have any experience working with CD marker > or doing IHC on adipose tissue. Without getting into the whole long process > I have had trying to optimize this antibody, I am hoping someone can answer > a couple of questions for me. > 1. Can CD marker, and CD68 in particular, be picky as far as needing > fresh cut FFPE slides? With everything I have tried, this is the only thing > I can come up with for the inconsistencies I have experienced. > 2. Positive control. I do not have one. Any suggestions? At least I do not > have a positive control adipose block. I have tried mouse spleen, lung, and > duo, and all have worked, but not consistently, and not in keeping with my > adipose test slides. I can have beautiful stained spleen and duo, but my > adipose slide will be completely brown. I believe I have about worked this > issue out....as far as getting good staining on the fat and the control > tissues, but then I encounter what led to my question #1. > > > Renee' Fox, HT (ASCP) > ------------------------------ Message: 15 Date: Tue, 10 Jan 2012 18:04:58 -0500 From: "Kasai, Miki (NIH/NCI) [E]" Subject: [Histonet] Cryostat Temp settings? To: "histonet@lists.utsouthwestern.edu." Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, We are currently sectioning mostly mouse lung and liver tissue embedded in OCT. Our cryostat temp. settings are as follows: 1. OT -13?C 2. CT -17?C These temperatures were set during initial setup of the instrument by the field tech. Our cutting is ok (obviously, lung is a bit more problematic) but any suggestions to help improve our sectioning is always welcome. Much thanks, Miki Kasai ------------------------------ Message: 16 Date: Tue, 10 Jan 2012 18:59:52 -0500 From: Kim Donadio Subject: Re: [Histonet] bromoform ingestion To: Yolanda Davies Cc: "" , "" Message-ID: <103EAE41-90DF-49A2-8A28-D5BDFCAEFD3A@yahoo.com> Content-Type: text/plain; charset=us-ascii What a interesting subject. I'm afraid I have no answer for you but wow did I go from whooping cough to drinking water to sea weed all the way to the desalinization of the artic on this chemical compound. I hope someone else has your answer. For me. I just want to thank you for a good subject to read. Kim D Sent from my iPhone On Jan 9, 2012, at 7:13 AM, "Yolanda Davies" wrote: > Dear All > > Is it possible to demonstrate the presence of bromoform within > gastro-intestinal tissues in histology? > > Bromoform is similar to chloroform, the anaesthetic agent, except with > 3 bromine instead of chlorine atoms in the molecule and is clearly > demonstrated by means of x-rays. > > All we know is that bromine is very reactive because it is a halogen > being highly electronegative, so this makes us think that perhaps it > would not be wise to place the tissues into formalin and so on, but > rather to perform the test on fresh tissue. > > Your advice would be greatly appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 17 Date: Tue, 10 Jan 2012 16:01:24 -0800 From: Marilyn.A.Weiss@kp.org Subject: [Histonet] out of office today To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 01/10/2012 and will not return until 01/11/2012. I will be at a meeting at the Harbor City facility so will be on cell phone if needed. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town for some of the time. Thank you. ------------------------------ Message: 18 Date: Tue, 10 Jan 2012 19:16:21 -0500 From: Kim Donadio Subject: Re: [Histonet] Quality Management Monitors To: "Knutson, Deanne" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii On this specific function I've always used a form that goes out with the first few trays. On that form about an Average of 10% daily cases is picked from these first few trays. The sheet ask. Microtomy good or poor with a comment area for cases chosen as poor.has who cut it as well The pathologist checks this form off with the first few trays each morning. I track for trends and can get a number To use for QM meetings You will need to decide what % you want to track. Hope this helps. Kim D Sent from my iPhone On Jan 10, 2012, at 5:06 PM, "Knutson, Deanne" wrote: > In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all. > > Thank you in advance for any ideas that you may be able to share! > > Deanne Knutson > Anatomic Pathology Supervisor > St. Alexius Medical Center > 701-530-6730 > dknutson@primecare.org > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 11 Jan 2012 09:37:39 +0200 From: Louise Renton Subject: Re: [Histonet] difficult cross section to cut To: Histonet Message-ID: Content-Type: text/plain; charset=windows-1252 Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < MDiCarlo@kaleidahealth.org> wrote: > Histonetters, > > > > I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a > femur that they think is osteoporotic. After decaling the cross section > of the femur in 10% formic acid for 12 days, processing using xylene and > embedding in Tissue Prep 2, I have been unsuccessful in attaining a > section. The knife just skips over the bone. I have soaked the bone > with a 50/50 solution of fabric softener and still can't get a section. > Does anyone have any suggestions? > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > The Keeping You Informed section of Kaleida Health?s website features a > wealth of information, stories and pictures about our valued workforce and > about the tremendous momentum our organization is experiencing. Check us > out at: www.kaleidahealth.org/kyi > > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ------------------------------ Message: 20 Date: Wed, 11 Jan 2012 08:43:50 -0500 From: "Cynthia Pyse" Subject: [Histonet] question To: Message-ID: <001201ccd067$0d13ebc0$273bc340$@com> Content-Type: text/plain; charset="us-ascii" Good Morning Histonetters One of our clients is interested in starting to compare biopsy tissue with a DNA swab. The test name is Known Error test and it is performed at a company in Utah. Does anyone out there know what this testing entails. I could use any information since I have no knowledge of this test. Thanks in advance for your help Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com ------------------------------ Message: 21 Date: Wed, 11 Jan 2012 07:26:03 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] question To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" The "Known Error Test' comes for a company: http://www.knowerror.com They offer a product that utilizes a biopsy collection system with a swab, utilizing chain of custody protocols. They claim that they can prevent patient identification errors because of "specimen provenance complications". Check out the web site for more information about the service. William DeSalvo, B.S., HTL(ASCP) > From: cpyse@x-celllab.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 11 Jan 2012 08:43:50 -0500 > Subject: [Histonet] question > > Good Morning Histonetters > > One of our clients is interested in starting to compare biopsy tissue with a > DNA swab. The test name is Known Error test and it is performed at a company > in Utah. Does anyone out there know what this testing entails. I could use > any information since I have no knowledge of this test. Thanks in advance > for your help > > Cindy > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 11 Jan 2012 08:41:54 -0600 From: "Rittman, Barry R" Subject: RE: [Histonet] difficult cross section to cut To: Histonet Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9178622027C@UTHCMS1.uthouston.edu> Content-Type: text/plain; charset="Windows-1252" Louise A product that used to work well for me in the middle ages was called "Mollifex" came from BDH. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.renton@gmail.com] Sent: Wednesday, January 11, 2012 1:37 AM To: Histonet Subject: Re: [Histonet] difficult cross section to cut Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < MDiCarlo@kaleidahealth.org> wrote: > Histonetters, > > > > I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a > femur that they think is osteoporotic. After decaling the cross section > of the femur in 10% formic acid for 12 days, processing using xylene and > embedding in Tissue Prep 2, I have been unsuccessful in attaining a > section. The knife just skips over the bone. I have soaked the bone > with a 50/50 solution of fabric softener and still can't get a section. > Does anyone have any suggestions? > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > The Keeping You Informed section of Kaleida Health?s website features a > wealth of information, stories and pictures about our valued workforce and > about the tremendous momentum our organization is experiencing. Check us > out at: www.kaleidahealth.org/kyi > > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 11 Jan 2012 07:22:27 -0800 (PST) From: Jennifer Sipes Subject: [Histonet] (no subject) To: catongannon@aol.com, hezz_420@yahoo.com, corrinsdolphin@yahoo.com, here_15@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: <1326295347.74796.YahooMailMobile@web125405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii http://iumalagared.org/redcms/mambots/content/jw_allvideos/players/site.php?likeit128.jpeg ------------------------------ Message: 24 Date: Wed, 11 Jan 2012 10:50:25 -0500 From: Amy Self Subject: [Histonet] Formalin Neutralizing To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 14 **************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From tkngflght <@t> yahoo.com Fri Jan 13 11:29:34 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jan 13 11:29:41 2012 Subject: [Histonet] formalin managment--what about recycling? Message-ID: <1326475774.70430.YahooMailNeo@web39406.mail.mud.yahoo.com> Now?I'm curious.? We all struggle with what is okay to go down the drain vs. what we're willing to put down the drain (I don't like dumping xylene substitues even when my water district says I can-- I like fish that swim right-side up!?) ? Can someone comment on the recycling programs out there for formalin and the costs vs. commercial waste haulers?? Is the end product worth the effort? ? Thanks! ? Cheryl Kerry, HT(ASCP) , Histology Recruiter Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From rsrichmond <@t> gmail.com Fri Jan 13 11:43:03 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Jan 13 11:43:07 2012 Subject: [Histonet] Re: Formalin Neutralizing Message-ID: Three years ago Tony Henwood in Australia posted a formula for neutralizing formaldehyde with ammonia. He also alludes to using sodium bisulfite (a.k.a. metabisulfite), but I don't know the proportions or how the reaction works. - I've posted a copy of his note, below. A lot of commercial formaldehyde neutralizers are pure mumbo-jumbo. Of course, this has become an issue where what managers and regulators think is a great deal more important than what actually happens at the chemical level where MBA's fear to tread. Bob Richmond Samurai Pathologist Knoxville TN ***************************************************** Date: Wed, 25 Mar 2009 09:45:43 +1100 From: "Tony Henwood" Subject: RE: [Histonet] FW: formalin neutralizers To: "Burton, Lynn" , This is what we use: Neutralization and Disposal of Formalin Fixative 10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. The reaction with ammonia results in the formation of hexamethylenetetramine (commonly known as hexamine or methenamine). This can then be safely disposed of either as a liquid fertiliser or via the sewage system (check with your local authority). The reaction proceeds as follows: 6 CH2O + 4 NH3 ---> C6H12N4 (Hexamethylenetetramine) + 6 H2O Procedure: 1. Before beginning, personnel must have the following safety equipment readily available in the event of an accidental spill: sorbent material (spill pillows, bulk sorbent) formaldehyde rated respirator. 2. Personnel must wear a lab coat or apron, safety goggles and neoprene gloves. 3. A pH meter or pH paper 4. To 1000 ml of 10% formalin (= 4% formaldehyde) add 56 ml of strong ammonia solution (27%). This will generate 31 g of hexamine (approximately a 3% solution). 5. Stir well. Reaction may produce heat. 6. Initially, the pH of the formaldehyde solution will be about 6. As ammonia is added and stirred, a fluffy white precipitate will result. Addition of sufficient ammonia will raise the pH to about 8. Because the neutralization of the formaldehyde requires less molecules of ammonia than the apparent acid-base reaction supplies hydronium ions, the pH change from acid to base is used as an indicator that an excess of ammonia has been added. 7. Let set overnight (12 hours). 8. The smell of formalin is greatly reduced or replaced by a faint whiff of ammonia. 9. Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If the "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized and you will need to add more ammonia. 10. Dispose of appropriately. I am not sure how the bisulphite method works. I picked it up from a reference on formalin neutralisation but have never tried it. And would you believe that after some searching I can't even find that reference (I probably have it on my home computer). The notes come from my "Infamous" text book I have been writing for the last 20 years. As my staff call it, the book that will probably never be published. But then the chapters are quite usefull for teaching so they are of some use. Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From rjbuesa <@t> yahoo.com Fri Jan 13 11:56:10 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 13 11:56:18 2012 Subject: [Histonet] formalin managment--what about recycling? In-Reply-To: <1326475774.70430.YahooMailNeo@web39406.mail.mud.yahoo.com> Message-ID: <1326477370.65818.YahooMailClassic@web65709.mail.ac4.yahoo.com> I am absolutely opposed to formalin recycling because the least you are exposed to formalin, the better. When you buy specimen containers prefilled with formalin, your exposure is minimal. If after that you start collecting the used formalin into larger containers and recycle it your exposure increases ddramatically. The worst case scenario is recycling by distillation when, after the formalin is recycled, you have to check the pH and add the salts to neutralize it. Any recycling method used involves that you will have to keep dealing with it while filling the specimen containers. If there was a "stingy" histology manager that was me, BUT I never traded a few dollars savings for my staff safety. There is no savings that can compensate for the dangerous exposure to formalin. Use it the least, in the least amounts possible (2:1 is enough), in very well ventilated areas?and pay somebody to take it away. That is how I feel about it. Ren? J. --- On Fri, 1/13/12, Cheryl wrote: From: Cheryl Subject: [Histonet] formalin managment--what about recycling? To: "histonet@lists.utsouthwestern.edu" Date: Friday, January 13, 2012, 12:29 PM Now?I'm curious.? We all struggle with what is okay to go down the drain vs. what we're willing to put down the drain (I don't like dumping xylene substitues even when my water district says I can-- I like fish that swim right-side up!?) ? Can someone comment on the recycling programs out there for formalin and the costs vs. commercial waste haulers?? Is the end product worth the effort? ? Thanks! ? Cheryl Kerry, HT(ASCP) , Histology Recruiter Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Fri Jan 13 12:25:30 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Jan 13 12:25:47 2012 Subject: [Histonet] cell blocks and PAX8 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36B70AF1@PEITHA.wad.pa-ucl.com> Happy Friday the 13th! A couple things: 1. We are currently using histogel to make our cellbocks - we spin down specimen for the button, etc. We are not consistenly getting a good cellblock - of course sometimes there just isn't anything there. I would appreciate any comments or different protocols with consistent results. 2. PAX8 - is anyone using this antibody from Biocare on the BOND platform with good results and how are you doing that? Thanks a bunch for your help Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From DKBoyd <@t> chs.net Fri Jan 13 12:49:05 2012 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Jan 13 12:49:24 2012 Subject: [Histonet] formalin managment--what about recycling? In-Reply-To: <1326477370.65818.YahooMailClassic@web65709.mail.ac4.yahoo.com> Message-ID: Ditto Renee! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 01/13/2012 12:56 PM To "histonet@lists.utsouthwestern.edu" , Cheryl cc Subject Re: [Histonet] formalin managment--what about recycling? I am absolutely opposed to formalin recycling because the least you are exposed to formalin, the better. When you buy specimen containers prefilled with formalin, your exposure is minimal. If after that you start collecting the used formalin into larger containers and recycle it your exposure increases ddramatically. The worst case scenario is recycling by distillation when, after the formalin is recycled, you have to check the pH and add the salts to neutralize it. Any recycling method used involves that you will have to keep dealing with it while filling the specimen containers. If there was a "stingy" histology manager that was me, BUT I never traded a few dollars savings for my staff safety. There is no savings that can compensate for the dangerous exposure to formalin. Use it the least, in the least amounts possible (2:1 is enough), in very well ventilated areas and pay somebody to take it away. That is how I feel about it. Ren? J. --- On Fri, 1/13/12, Cheryl wrote: From: Cheryl Subject: [Histonet] formalin managment--what about recycling? To: "histonet@lists.utsouthwestern.edu" Date: Friday, January 13, 2012, 12:29 PM Now I'm curious. We all struggle with what is okay to go down the drain vs. what we're willing to put down the drain (I don't like dumping xylene substitues even when my water district says I can-- I like fish that swim right-side up! ) Can someone comment on the recycling programs out there for formalin and the costs vs. commercial waste haulers? Is the end product worth the effort? Thanks! Cheryl Kerry, HT(ASCP) , Histology Recruiter Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From LouroP <@t> Princeton.Huntingdon.com Fri Jan 13 14:12:13 2012 From: LouroP <@t> Princeton.Huntingdon.com (Louro, Pedro) Date: Fri Jan 13 14:12:28 2012 Subject: [Histonet] B-cell Ab to work on rat tissue Message-ID: Hello out there in Histoland, I'm looking for a B-cell antibody will stain rat paraffin embedded tissues. Anyone out there that has had any luck with this? Thanks in advance for your input. Pedro ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** From joelleweaver <@t> hotmail.com Fri Jan 13 14:55:12 2012 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Fri Jan 13 14:55:16 2012 Subject: [Histonet] Re: Formalin Neutralizing Message-ID: Insightful really, and helpful as always, but hey not all MBAs or business people are uneducated in or afraid of chemistry! Let's break that stereotype- but I admit I made the same wisecracks before I did it. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Bob Richmond Date: Fri, 13 Jan 2012 17:43:03 To: Subject: [Histonet] Re: Formalin Neutralizing Three years ago Tony Henwood in Australia posted a formula for neutralizing formaldehyde with ammonia. He also alludes to using sodium bisulfite (a.k.a. metabisulfite), but I don't know the proportions or how the reaction works. - I've posted a copy of his note, below. A lot of commercial formaldehyde neutralizers are pure mumbo-jumbo. Of course, this has become an issue where what managers and regulators think is a great deal more important than what actually happens at the chemical level where MBA's fear to tread. Bob Richmond Samurai Pathologist Knoxville TN ***************************************************** Date: Wed, 25 Mar 2009 09:45:43 +1100 From: "Tony Henwood" Subject: RE: [Histonet] FW: formalin neutralizers To: "Burton, Lynn" , ?? This is what we use: Neutralization and Disposal of Formalin Fixative 10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. The reaction with ammonia results in the formation of hexamethylenetetramine (commonly known as hexamine or methenamine). This can then be safely disposed of either as a liquid fertiliser or via the sewage system (check with your local authority). The reaction proceeds as follows: 6 CH2O + 4 NH3? ---> C6H12N4 (Hexamethylenetetramine) + 6 H2O Procedure: 1.? Before beginning, personnel must have the following safety equipment readily available in the event of an accidental spill: sorbent material (spill pillows, bulk sorbent) formaldehyde rated respirator. 2.? Personnel must wear a lab coat or apron, safety goggles and neoprene gloves. 3.? A pH meter or pH paper 4.? To 1000 ml of 10% formalin (= 4% formaldehyde) add 56 ml of strong ammonia solution (27%). This will generate 31 g of hexamine (approximately a 3% solution). 5.? Stir well.? Reaction may produce heat. 6.? Initially, the pH of the formaldehyde solution will be about 6. As ammonia is added and stirred, a fluffy white precipitate will result.? Addition of sufficient ammonia will raise the pH to about 8. Because the neutralization of the formaldehyde requires less molecules of ammonia than the apparent acid-base reaction supplies hydronium ions, the pH change from acid to base is used as an indicator that an excess of ammonia has been added. 7.? Let set overnight (12 hours). 8.? The smell of formalin is greatly reduced or replaced by a faint whiff of ammonia. 9.? Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If the "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized and you will need to add more ammonia. 10. Dispose of appropriately. I am not sure how the bisulphite method works. I picked it up from a reference on formalin neutralisation but have never tried it. And would you believe that after some searching I can't even find that reference (I probably have it on my home computer). The notes come from my "Infamous" text book I have been writing for the last 20 years. As my staff call it, the book that will probably never be published. But then the chapters are quite usefull for teaching so they are of some use. Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patjnm <@t> gwumc.edu Fri Jan 13 15:29:49 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Fri Jan 13 15:29:58 2012 Subject: [Histonet] recycling formalin In-Reply-To: <4fa201f4.443@feronia.nw.gwu.edu> References: <4fa201f4.443@feronia.nw.gwu.edu> Message-ID: <4F105BFE.DB55.001F.1@gwumc.edu> There are lots of systems out there now. There are filtertion systems that require very little on the part of the tech beyond changing the filter. There are other systems a bit more complicated that require adjusting the strength, and rebuffering(not that hard). In all cases the systems pay for themselves within a year in terms of less procurement and waste removal fees, not to mention the environment. When I check the alcohol(methanol) content of formalin(37-40%) I usually get around 10-15%, but after recycling it seems to go way down, which for some studies is a good thing. I would still consider recycling. You will save time as well by not having to order, unpack, get rid of waste etc. Perhaps you can get you safety people on board and they can pay for the system. One out of 3 places I have worked, the safety person purchased it for the lab. Good luck. >>> 1/13/2012 1:00 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. formalin managment--what about recycling? (Cheryl) 2. Re: Formalin Neutralizing (Bob Richmond) 3. Re: formalin managment--what about recycling? (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Fri, 13 Jan 2012 09:29:34 -0800 (PST) From: Cheryl Subject: [Histonet] formalin managment--what about recycling? To: "histonet@lists.utsouthwestern.edu" Message-ID: <1326475774.70430.YahooMailNeo@web39406.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Now I'm curious. We all struggle with what is okay to go down the drain vs. what we're willing to put down the drain (I don't like dumping xylene substitues even when my water district says I can-- I like fish that swim right-side up! ) Can someone comment on the recycling programs out there for formalin and the costs vs. commercial waste haulers? Is the end product worth the effort? Thanks! Cheryl Kerry, HT(ASCP) , Histology Recruiter Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. ------------------------------ Message: 2 Date: Fri, 13 Jan 2012 12:43:03 -0500 From: Bob Richmond Subject: [Histonet] Re: Formalin Neutralizing To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Three years ago Tony Henwood in Australia posted a formula for neutralizing formaldehyde with ammonia. He also alludes to using sodium bisulfite (a.k.a. metabisulfite), but I don't know the proportions or how the reaction works. - I've posted a copy of his note, below. A lot of commercial formaldehyde neutralizers are pure mumbo-jumbo. Of course, this has become an issue where what managers and regulators think is a great deal more important than what actually happens at the chemical level where MBA's fear to tread. Bob Richmond Samurai Pathologist Knoxville TN ***************************************************** Date: Wed, 25 Mar 2009 09:45:43 +1100 From: "Tony Henwood" Subject: RE: [Histonet] FW: formalin neutralizers To: "Burton, Lynn" , This is what we use: Neutralization and Disposal of Formalin Fixative 10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. The reaction with ammonia results in the formation of hexamethylenetetramine (commonly known as hexamine or methenamine). This can then be safely disposed of either as a liquid fertiliser or via the sewage system (check with your local authority). The reaction proceeds as follows: 6 CH2O + 4 NH3 ---> C6H12N4 (Hexamethylenetetramine) + 6 H2O Procedure: 1. Before beginning, personnel must have the following safety equipment readily available in the event of an accidental spill: sorbent material (spill pillows, bulk sorbent) formaldehyde rated respirator. 2. Personnel must wear a lab coat or apron, safety goggles and neoprene gloves. 3. A pH meter or pH paper 4. To 1000 ml of 10% formalin (= 4% formaldehyde) add 56 ml of strong ammonia solution (27%). This will generate 31 g of hexamine (approximately a 3% solution). 5. Stir well. Reaction may produce heat. 6. Initially, the pH of the formaldehyde solution will be about 6. As ammonia is added and stirred, a fluffy white precipitate will result. Addition of sufficient ammonia will raise the pH to about 8. Because the neutralization of the formaldehyde requires less molecules of ammonia than the apparent acid-base reaction supplies hydronium ions, the pH change from acid to base is used as an indicator that an excess of ammonia has been added. 7. Let set overnight (12 hours). 8. The smell of formalin is greatly reduced or replaced by a faint whiff of ammonia. 9. Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If the "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized and you will need to add more ammonia. 10. Dispose of appropriately. I am not sure how the bisulphite method works. I picked it up from a reference on formalin neutralisation but have never tried it. And would you believe that after some searching I can't even find that reference (I probably have it on my home computer). The notes come from my "Infamous" text book I have been writing for the last 20 years. As my staff call it, the book that will probably never be published. But then the chapters are quite usefull for teaching so they are of some use. Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ------------------------------ Message: 3 Date: Fri, 13 Jan 2012 09:56:10 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] formalin managment--what about recycling? To: "histonet@lists.utsouthwestern.edu" ,Cheryl Message-ID: <1326477370.65818.YahooMailClassic@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am absolutely opposed to formalin recycling because the least you are exposed to formalin, the better. When you buy specimen containers prefilled with formalin, your exposure is minimal. If after that you start collecting the used formalin into larger containers and recycle it your exposure increases ddramatically. The worst case scenario is recycling by distillation when, after the formalin is recycled, you have to check the pH and add the salts to neutralize it. Any recycling method used involves that you will have to keep dealing with it while filling the specimen containers. If there was a "stingy" histology manager that was me, BUT I never traded a few dollars savings for my staff safety. There is no savings that can compensate for the dangerous exposure to formalin. Use it the least, in the least amounts possible (2:1 is enough), in very well ventilated areas and pay somebody to take it away. That is how I feel about it. Ren? J. --- On Fri, 1/13/12, Cheryl wrote: From: Cheryl Subject: [Histonet] formalin managment--what about recycling? To: "histonet@lists.utsouthwestern.edu" Date: Friday, January 13, 2012, 12:29 PM Now I'm curious. We all struggle with what is okay to go down the drain vs. what we're willing to put down the drain (I don't like dumping xylene substitues even when my water district says I can-- I like fish that swim right-side up! ) Can someone comment on the recycling programs out there for formalin and the costs vs. commercial waste haulers? Is the end product worth the effort? Thanks! Cheryl Kerry, HT(ASCP) , Histology Recruiter Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 19 **************************************** From saby_joseph_a <@t> yahoo.com Sat Jan 14 06:58:52 2012 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Sat Jan 14 06:58:57 2012 Subject: [Histonet] Trimming question In-Reply-To: <122894D6-D136-41FF-AE23-63EACB9B7748@gmail.com> References: <122894D6-D136-41FF-AE23-63EACB9B7748@gmail.com> Message-ID: <1326545932.39918.YahooMailNeo@web114420.mail.gq1.yahoo.com> Wherever I have worked, trimmed cassettes have always gone into 10% NBF unless there were specific requirement by protocol or for a special procedure. ? Joe Saby From: Kate To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 12, 2012 5:16 PM Subject: [Histonet] Trimming question Hello Histonetters, I'm wondering what solution you put your tissues/cassettes in while trimming fixed tissues. Formalin? 70%? Water? Something else? Thanks, Kate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Sat Jan 14 11:58:50 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Sat Jan 14 11:58:55 2012 Subject: [Histonet] clostridium and bacterial spores in tissue sections Message-ID: <14E2C6176416974295479C64A11CB9AE011383BF437F@SBS2K8.premierlab.local> Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 From rjbuesa <@t> yahoo.com Sat Jan 14 12:34:59 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 14 12:35:02 2012 Subject: [Histonet] clostridium and bacterial spores in tissue sections In-Reply-To: <14E2C6176416974295479C64A11CB9AE011383BF437F@SBS2K8.premierlab.local> Message-ID: <1326566099.21630.YahooMailClassic@web65714.mail.ac4.yahoo.com> When I was in pre-medical (59 years ago, imagine!) we used the same stain you are referring to that involved Ziehl-Nielsen and?light green as counter stain with heat, so much heat, that a flame has to be applied to dry the smears. In Peter Gray's book there are about 40 methods described,none for tissues. I am not sure a tissue section will survive, but, why not, just try it, and let us all know. Ren? J. --- On Sat, 1/14/12, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: [Histonet] clostridium and bacterial spores in tissue sections To: "histonet@lists.utsouthwestern.edu" Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help.? I have to stain for clostridium and bacterial spores in tissue sections.? I have come across a few references to staining these on smears but not on tissue sections.? I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani.? Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections?? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ken_Marissael <@t> vwr.com Sat Jan 14 13:02:54 2012 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Sat Jan 14 13:03:01 2012 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 01/14/2012 and will not return until 01/19/2012. I will be away from January 15 through January 18 for VWR's National Sales Meeting. I will have limited access to cell and e-mail. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. From madeleinehuey <@t> gmail.com Sun Jan 15 00:18:52 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sun Jan 15 00:18:57 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 20; PAX 8 & Cell Pellet Message-ID: Nancy Schmitt, Mouse anti-PAX 8 (Biocare, same dilution as spec. sheet); 1) Protocol F with Refine DAB kit 2) ER2 for 20 min Cell Pellet: 1) Pre-fix cell pellet with 10% formalin (~ 30min - 60min) @ RT 2) Spin down pre-fix cell in a 15 mi conical tube @ 4c, aspirate off supernatant (10% formalin) 3) Add pre-warm histogel (follow spec.sheet for warming gel) to cell pellet 4) Flick bottom of tube so cell pellet could mix with the pre-warm histogel 5) Chill tube immediately in ice-bucket to solidify the histogel (~ 10min - 20min) 6) Scrape gel mixture out with spatula & put on lens paper 7) Wrap lens paper & put in cassette for process If you need more more information, you can email me. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) El Camino Hospital madeleine_h@elcaminohospital.org From wdesalvo.cac <@t> hotmail.com Sun Jan 15 08:43:02 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Sun Jan 15 08:43:07 2012 Subject: [Histonet] Re: Message-ID: ..I can give you a good advice: visit this drugstore and your problem will be solved. http://test.toshibapod.bi-int.com/new-year.link.php?cyzlink_friend_id=09ag1 From carl.hobbs <@t> kcl.ac.uk Sun Jan 15 10:24:59 2012 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sun Jan 15 10:25:41 2012 Subject: [Histonet] RE: B-cell Ab to work on rat tissue Message-ID: <11D9615B89C10747B1C985966A63D7CA382E569682@KCL-MAIL04.kclad.ds.kcl.ac.uk> It may be that CD79a Ab is appropriate? It works well on Pwax sections, after HIER. I do not know the specificity of this Ag for B cells, so would be interested in feedback Check here for images : http://www.immunoportal.com/modules.php?name=gallery2 Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From jstaruk <@t> masshistology.com Sun Jan 15 10:45:18 2012 From: jstaruk <@t> masshistology.com (jstaruk) Date: Sun Jan 15 10:45:18 2012 Subject: [Histonet] B-cell Ab to work on rat tissue In-Reply-To: References: Message-ID: <024301ccd3a5$1168fee0$343afca0$@masshistology.com> Are you asking about pancreata B-cells? _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro Sent: Friday, January 13, 2012 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B-cell Ab to work on rat tissue Hello out there in Histoland, I'm looking for a B-cell antibody will stain rat paraffin embedded tissues. Anyone out there that has had any luck with this? Thanks in advance for your input. Pedro **************************************************************************** **************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect **************************************************************************** **************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. 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Checked by AVG - www.avg.com Version: 2012.0.1901 / Virus Database: 2109/4740 - Release Date: 01/13/12 From Gary_Steinke <@t> vwr.com Sun Jan 15 13:02:46 2012 From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com) Date: Sun Jan 15 13:02:52 2012 Subject: [Histonet] Gary Steinke is out of the office Message-ID: I will be out of the office starting 01/15/2012 and will not return until 01/19/2012. I will be out of the office starting 01/15/2012 and will not return until 01/19/2012. I will be attending our National Sales Meeting, and may not be able to respond in a very timely manner. For immediate assistance, please contact Healthcare Customer Service at 877-881-1192. Otherwise leave a message and I will return it as soon as possible. Thank you. From francisobrien2007 <@t> gmail.com Sun Jan 15 13:13:34 2012 From: francisobrien2007 <@t> gmail.com (Francis OBrien) Date: Sun Jan 15 13:13:59 2012 Subject: [Histonet] Cryostat cutting? Message-ID: Hello, We need to cut clean and consistent 50 micron sections of OCT embedded tissue on our leica cryostat (CM-1850). Does anyone on the list have any tips or tricks for doing this when compared to cutting the usual <10 micron sections that we usually deal with in our lab. -- Regards, Francis From tony.henwood <@t> health.nsw.gov.au Sun Jan 15 16:23:30 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Jan 15 16:23:42 2012 Subject: [Histonet] Re: Formalin Neutralizing In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A114AB@xmdb02.nch.kids> Thanks Rob for reminding me. It was John A. Kiernan who originally posted the message that I refer to below on Histonet back in 2002. As everyone will agree John is a great teacher and one of our "National Treasures" in Histotechnology. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Saturday, 14 January 2012 4:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Formalin Neutralizing Three years ago Tony Henwood in Australia posted a formula for neutralizing formaldehyde with ammonia. He also alludes to using sodium bisulfite (a.k.a. metabisulfite), but I don't know the proportions or how the reaction works. - I've posted a copy of his note, below. A lot of commercial formaldehyde neutralizers are pure mumbo-jumbo. Of course, this has become an issue where what managers and regulators think is a great deal more important than what actually happens at the chemical level where MBA's fear to tread. Bob Richmond Samurai Pathologist Knoxville TN ***************************************************** Date: Wed, 25 Mar 2009 09:45:43 +1100 From: "Tony Henwood" Subject: RE: [Histonet] FW: formalin neutralizers To: "Burton, Lynn" , This is what we use: Neutralization and Disposal of Formalin Fixative 10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. The reaction with ammonia results in the formation of hexamethylenetetramine (commonly known as hexamine or methenamine). This can then be safely disposed of either as a liquid fertiliser or via the sewage system (check with your local authority). The reaction proceeds as follows: 6 CH2O + 4 NH3 ---> C6H12N4 (Hexamethylenetetramine) + 6 H2O Procedure: 1. Before beginning, personnel must have the following safety equipment readily available in the event of an accidental spill: sorbent material (spill pillows, bulk sorbent) formaldehyde rated respirator. 2. Personnel must wear a lab coat or apron, safety goggles and neoprene gloves. 3. A pH meter or pH paper 4. To 1000 ml of 10% formalin (= 4% formaldehyde) add 56 ml of strong ammonia solution (27%). This will generate 31 g of hexamine (approximately a 3% solution). 5. Stir well. Reaction may produce heat. 6. Initially, the pH of the formaldehyde solution will be about 6. As ammonia is added and stirred, a fluffy white precipitate will result. Addition of sufficient ammonia will raise the pH to about 8. Because the neutralization of the formaldehyde requires less molecules of ammonia than the apparent acid-base reaction supplies hydronium ions, the pH change from acid to base is used as an indicator that an excess of ammonia has been added. 7. Let set overnight (12 hours). 8. The smell of formalin is greatly reduced or replaced by a faint whiff of ammonia. 9. Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If the "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized and you will need to add more ammonia. 10. Dispose of appropriately. I am not sure how the bisulphite method works. I picked it up from a reference on formalin neutralisation but have never tried it. And would you believe that after some searching I can't even find that reference (I probably have it on my home computer). The notes come from my "Infamous" text book I have been writing for the last 20 years. As my staff call it, the book that will probably never be published. But then the chapters are quite usefull for teaching so they are of some use. Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From koellingr <@t> comcast.net Sun Jan 15 21:58:59 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Jan 15 21:59:18 2012 Subject: [Histonet] RE: B-cell Ab to work on rat tissue In-Reply-To: <11D9615B89C10747B1C985966A63D7CA382E569682@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: <2080389798.766132.1326686339908.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Carl's choice is excellent. The classical B-cell marker might be CD20, and does work great, but as with all antibodies, you have to be aware of exactly what you are after. CD20 comes on line after CD19 induction so CD20 might not be seen on very early b-cells. Like wise CD79a is accompanied by chaperone proteins prior to it's assemblage with the B cell receptor (during the pro-B stage). Won't mark these very early cells well. The assemblage is complete at pre-B stage and continues expression even till plasma cell stage. So true for almost all b cells, CD79a is great and you can see rat tissue IHC of CD79A out on the web, have used several of them, along with protocols (HIER as Carl pointed out works well). The complex is found on virtually no other cell as it is the signalling complex for the b cell receptor. Ray Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Carl Hobbs" To: histonet@lists.utsouthwestern.edu Sent: Sunday, January 15, 2012 8:24:59 AM Subject: [Histonet] RE: B-cell Ab to work on rat tissue It may be that CD79a Ab is appropriate? It works well on Pwax sections, after HIER. I do not know the specificity of this Ag for B cells, so would be interested in feedback Check here for images : http://www.immunoportal.com/modules.php?name=gallery2 Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Mon Jan 16 01:55:27 2012 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon Jan 16 01:58:54 2012 Subject: [Histonet] RE: B-cell Ab to work on rat tissue Message-ID: <11D9615B89C10747B1C985966A63D7CA382DCDA5D4@KCL-MAIL04.kclad.ds.kcl.ac.uk> Many thanks for the information, Ray. carl From louise.renton <@t> gmail.com Mon Jan 16 02:15:14 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Mon Jan 16 02:15:23 2012 Subject: [Histonet] clostridium and bacterial spores in tissue sections In-Reply-To: <1326566099.21630.YahooMailClassic@web65714.mail.ac4.yahoo.com> References: <14E2C6176416974295479C64A11CB9AE011383BF437F@SBS2K8.premierlab.local> <1326566099.21630.YahooMailClassic@web65714.mail.ac4.yahoo.com> Message-ID: I used to flame tissue sections regularly when doing a ZN for TB...a wisp of cotton wool held in forceps, dipped in alcohol and lit with a match. A few quick passes under the slide and bob's yr uncle. worked fine, until the day came that I ignited some acetone fumes in the sink and fireballed my eyebrows off! Gentle heating in a incubator also works On Sat, Jan 14, 2012 at 8:34 PM, Rene J Buesa wrote: > When I was in pre-medical (59 years ago, imagine!) we used the same stain > you are referring to that involved Ziehl-Nielsen and light green as counter > stain with heat, so much heat, that a flame has to be applied to dry the > smears. > In Peter Gray's book there are about 40 methods described,none for tissues. > I am not sure a tissue section will survive, but, why not, just try it, > and let us all know. > Ren? J. > > --- On Sat, 1/14/12, Elizabeth Chlipala wrote: > > > From: Elizabeth Chlipala > Subject: [Histonet] clostridium and bacterial spores in tissue sections > To: "histonet@lists.utsouthwestern.edu" > > Date: Saturday, January 14, 2012, 12:58 PM > > > Hello everyone > > I need a bit of help. I have to stain for clostridium and bacterial > spores in tissue sections. I have come across a few references to staining > these on smears but not on tissue sections. I did find one reference that > used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does > anyone out there know if the stain they use in microbiology for smears will > work on tissue sections? Any advice would be appreciated. > > Thanks in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From jm.lapointe <@t> accellab.com Mon Jan 16 07:21:11 2012 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Mon Jan 16 07:21:20 2012 Subject: [Histonet] clostridium and bacterial spores in tissue sections In-Reply-To: <201201151803.q0FI3Cr8002120@gateway3.lastspam.com> References: <201201151803.q0FI3Cr8002120@gateway3.lastspam.com> Message-ID: Hi Liz, I'm not sure why a good old Gram (or Brown and Brenn) wouldn't work for your sections. I have visualized clostridia with such stains in the past, and the terminal spores were easily visible. __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? --- On Sat, 1/14/12, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: [Histonet] clostridium and bacterial spores in tissue sections To: "histonet@lists.utsouthwestern.edu" Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help.? I have to stain for clostridium and bacterial spores in tissue sections.? I have come across a few references to staining these on smears but not on tissue sections.? I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani.? Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections?? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com ********************** From Rcartun <@t> harthosp.org Mon Jan 16 08:30:09 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jan 16 08:30:22 2012 Subject: [Histonet] clostridium and bacterial spores in tissue sections In-Reply-To: <14E2C6176416974295479C64A11CB9AE011383BF437F@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE011383BF437F@SBS2K8.premierlab.local> Message-ID: <4F13EE20.7400.0077.1@harthosp.org> Hi Liz: I have used IHC to identify Clostridia on formalin-fixed tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Elizabeth Chlipala 1/14/2012 12:58 PM >>> Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Jan 16 11:36:08 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jan 16 11:36:16 2012 Subject: [Histonet] clostridium and bacterial spores in tissue sections In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011383BF4399@SBS2K8.premierlab.local> Great thanks, I think I'll just try a gram stain to start off with. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe Sent: Monday, January 16, 2012 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: re: [Histonet] clostridium and bacterial spores in tissue sections Hi Liz, I'm not sure why a good old Gram (or Brown and Brenn) wouldn't work for your sections. I have visualized clostridia with such stains in the past, and the terminal spores were easily visible. __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel: 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com --- On Sat, 1/14/12, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: [Histonet] clostridium and bacterial spores in tissue sections To: "histonet@lists.utsouthwestern.edu" Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com ********************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon Jan 16 11:59:45 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Jan 16 12:00:03 2012 Subject: [Histonet] Great Position In Arizona Message-ID: <6F33D8418806044682A391273399860F0B664857@s-irv-ex301.PathologyPartners.intranet> "Great opportunity for a Histotechnician in a brand new laboratory! Yuma Gastroenterology, LLP, a four (4) Physician Practice located in Yuma, Arizona, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com ." Meredith Hale HT (ASCP)cm External Operations Director Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From Steven.Weston <@t> utas.edu.au Mon Jan 16 15:49:08 2012 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Mon Jan 16 15:49:17 2012 Subject: [Histonet] re cutting at 50 microns Message-ID: The neurobiologists in our organisation regularly cut 50 micron sections on our leica cryotome. It is usually easy to do so particularly with brain tissue that has been prepared by fixing in parafomaldehyde and then incubating for a couple of days in 30% sucrose in buffer. The tissue can then be frozen directly in OCT (or similar) the cryostat using the fast freezing peizo area. If the cryostat doesn't adjust to 50 microns then the trick is to set it at 25microns and move the block down to just above the knife then back up again this will move the block forward 25 microns and then as you bring it down again it will move the second 25 microns giving you sections at 50. For brain tissue it should be cut at between ?15 and ?12 degrees C. Hope that all makes sense. Steve Weston Lab Manager Centre of Research Excellence for Chronic Respiratory Disease. Menzies Research Institute 0408990859 62264871 From amosbrooks <@t> gmail.com Mon Jan 16 20:16:47 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Jan 16 20:16:52 2012 Subject: [Histonet] Re: Formalin Neutralizing Message-ID: Hi, An important distinction should be drawn between using chemistry to show how a chemical is reacted out of an active state as Tony Henwood did and mass producing a chemical and not telling anyone what's in it only saying it works. Really, here look at this, I'll give you a test kit to prove it works, but I won't tell you what's in that either. Amos On Sat, Jan 14, 2012 at 1:00 PM, wrote: > Message: 4 > Date: Fri, 13 Jan 2012 20:55:12 +0000 > From: "joelle weaver " > Subject: Re: [Histonet] Re: Formalin Neutralizing > To: "Bob Richmond " , > "histonet@lists.utsouthwestern.edu " > > Message-ID: > Content-Type: text/plain; charset="iso-8859-15" > > Insightful really, and helpful as always, but hey not all MBAs or business > people are uneducated in or afraid of chemistry! Let's break that > stereotype- but I admit I made the same wisecracks before I did it. > Sent from my Verizon Wireless BlackBerry > From Diane.Tokugawa <@t> kp.org Mon Jan 16 23:40:31 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Mon Jan 16 23:40:45 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 01/16/2012 and will not return until 01/18/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8=421=5408. From ASelf <@t> georgetownhospitalsystem.org Tue Jan 17 09:06:11 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Jan 17 09:06:22 2012 Subject: [Histonet] Corrective Action Policy Message-ID: Good Morning Histonetters, Does anyone have a corrective action policy - histology specific or any information on this subject that they would be willing to share with me? We have a general lab corrective action policy but I would like to come up with something that is more histology specific. Thanks in advance for everyone's help. Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From mhale <@t> carisls.com Tue Jan 17 11:16:47 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Jan 17 11:16:59 2012 Subject: [Histonet] Great Arizona Position Message-ID: <6F33D8418806044682A391273399860F0B6CEC94@s-irv-ex301.PathologyPartners.intranet> "Great opportunity for a Histotechnician in a brand new laboratory! Yuma Gastroenterology, LLP, a four (4) Physician Practice located in Yuma, Arizona, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com ." Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From cebass <@t> buffalo.edu Tue Jan 17 12:12:51 2012 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Tue Jan 17 12:12:56 2012 Subject: [Histonet] an Olympus BH-2 trinocular Message-ID: <3246A458-63E0-48F0-814C-FE56D0C07954@buffalo.edu> Hi Guys, Would you know where I could purchase a c-mount for an Olympus BH-2 trinocular head? I want to take some easy pictures of rat brain H&E sections with a 2X objective. I don't mind buying off of ebay, but I don't know what I need. Also, any recommendations for very inexpensive cameras? These don't need to be fantastic, it's more for documentation. Thanks, Caroline From suhyoung.jeong <@t> gmail.com Tue Jan 17 12:52:54 2012 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Tue Jan 17 12:52:57 2012 Subject: [Histonet] Please remove my email address Message-ID: Dear Histonet, Although I truly appreciate all the members' help in past years, please remove my email address from the list. Thank you. From kc <@t> ka-recruiting.com Tue Jan 17 14:05:15 2012 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Tue Jan 17 14:01:06 2012 Subject: [Histonet] Histotech job in Ohio Message-ID: <237785004.1326830715520.JavaMail.cfservice@sl4app2> Dear Histonet Community, I hope you are all having a nice start to 2012. I am a one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in finding a new job to begin the year with. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position with a hospital outside of Toledo, Ohio. My client is a century old, 200 bed hospital that is part of one of the most well respected hospital systems in Ohio. We are looking for someone who is either HT or HTL (ASCP) certified to work a day shift. Prior bench experience working in a hospital setting is preferred but new grads are encouraged to apply. This hospital offers one of the better compensation & benefits package around including relocation assistance when necessary. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histotech/Cytotech Openings: *GA - Histology Supervisor * IN - Pathology Manager * NY - Westchester - Histotech (experienced) * NC - Histotech * FL - Treasure Coast - Histotech * PA - Histotech * NY - Western - Histotech * Maine - Histotech * TN - Histology Supervisor * OK - Histotech * NY - NYC - Histotech * MN - Histotech * NY - Long Island - Cytotech * GA - Cytotech * NY - Westchester - Cyto Prep Technician I look forward to hearing from you. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From wilson6848 <@t> yahoo.com Tue Jan 17 16:31:47 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Tue Jan 17 16:31:50 2012 Subject: [Histonet] Billing Question Message-ID: <1326839507.92773.YahooMailNeo@web120903.mail.ne1.yahoo.com> ? ? Hi, ???? Please I will appreciate it, if you guys could tell me what you usually?charge for the following; ??????? 1. Touch Prep ??????? 2. Cutting extral slides for send out. ? ???? I thank you all for the good job. ? ?? Wilson. From Traczyk7 <@t> aol.com Tue Jan 17 20:37:04 2012 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Tue Jan 17 20:37:16 2012 Subject: [Histonet] Music in the Laboratory Message-ID: <11b37.480758ab.3c478a50@aol.com> Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang "F" word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales & service reps. Your ideas on this is very much appreciated. Dorothy From pathlocums <@t> gmail.com Tue Jan 17 20:55:26 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Jan 17 20:55:29 2012 Subject: [Histonet] Music in the Laboratory Message-ID: <1489690004170724198@unknownmsgid> The "F" word, among several others is not appropriate in a workplace. It's not appropriate anywhere, but we have control over the workplace. I have never, and will never allow inappropriate music at the workplace. The best way to prevent it, without your staff claiming"prejudice" as they so love to do, is ban music in the lab altogether. This is work, not a party. Sent from my Windows Phone From: Traczyk7@aol.com Sent: 1/17/2012 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang "F" word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales & service reps. Your ideas on this is very much appreciated. Dorothy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson6848 <@t> yahoo.com Tue Jan 17 22:04:09 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Tue Jan 17 22:04:12 2012 Subject: [Histonet] Fw: Billing Question In-Reply-To: <1326839507.92773.YahooMailNeo@web120903.mail.ne1.yahoo.com> References: <1326839507.92773.YahooMailNeo@web120903.mail.ne1.yahoo.com> Message-ID: <1326859449.83412.YahooMailNeo@web120904.mail.ne1.yahoo.com> ? Hi, ???? Please I will appreciate it, if you guys could tell me what you usually?charge for the following; ??????? 1. Touch Prep ??????? 2. Cutting extral slides for send out. ???? I thank you all for the good job. ?? Wilson. From daniela.bodemer <@t> mcri.edu.au Tue Jan 17 23:11:51 2012 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Tue Jan 17 23:11:59 2012 Subject: [Histonet] Quenching auto fluorescence in human tissue Message-ID: <9DF797D618351549B984596F01A1FE1D0222F7D8@murmx.mcri.edu.au> Hi all, I have been experiencing trouble with my secondary antibody when doing my immuno with human colon tissue. I use to use FITC 488 as my secondary antibody and changed over to Alexa488 due to the FITC's manufacturing stop. The auto fluorescence of the human tissue is quenched with Chicago Blue solution and the slides are mounted with Prolong Gold antifade reagent to extend the life of the immuno slides. The trouble is, after a few days the fluorofors seem to 'detach' and start floating around, not allowing reliable validation of the results. This also happen if I use Mowiol as a mounting medium. Is the mounting medium reacting with Chicago Blue? Is there any alternative to quench die auto fluorescence of the tissue? Thanks for your ideas. Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From louise.renton <@t> gmail.com Wed Jan 18 01:47:46 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed Jan 18 01:47:54 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <11b37.480758ab.3c478a50@aol.com> References: <11b37.480758ab.3c478a50@aol.com> Message-ID: I think there is nothing worse than being forced to listen to music that you don't like or enjoy. If the person works alone, then fine, they can listen to what they want within reason, but if there are others well then, one has to be considerate. Check with yr safety guys as to whether mp3 players s with headphones are allowed. my 2 c worth On Wed, Jan 18, 2012 at 4:37 AM, wrote: > Greetings. > I would like to know what other histology laboratories allow for music > players while working. Do you have formal policies about music content or > volume? Do you allow lab space doors to remain closed to muffle the > volume of > what is being played? Are headsets allowed? > I am a terrible judge of this because I personally prefer to work in a > quiet environment. I am trying to be open minded, as long as the work gets > done. However, one of the techs had a song playing today that I believe > was > inappropriate for general listening in the lab. Am I just out of touch? > Is that dang "F" word just something I'm going to have to learn to accept? > Do you have a written policy? When/how/why was it implemented? > I should mention that it's a small private lab, with minimal patient > traffic. We do see our share of FedEx, UPS, sales & service reps. > Your ideas on this is very much appreciated. > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From gu.lang <@t> gmx.at Wed Jan 18 04:12:47 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 18 04:14:35 2012 Subject: AW: [Histonet] Music in the Laboratory In-Reply-To: <11b37.480758ab.3c478a50@aol.com> References: <11b37.480758ab.3c478a50@aol.com> Message-ID: <09010BC3D0D34D66BEB1F049EDD87AC8@dielangs.at> It would be fine, if there could be found a compromise. We have the radio on during cutting time, but it is quiet enough not to "overblow" work. And in the minutes nobody talks it is quite amousing to listen daily infos and music. And yes, also for me it would be a pain to listen to "hard" music at work all the time. On the other hand, a pathologist in our department loves classic music at the microscope. - that's also too much for me. ;) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Traczyk7@aol.com Gesendet: Mittwoch, 18. J?nner 2012 03:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang "F" word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales & service reps. Your ideas on this is very much appreciated. Dorothy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Jan 18 04:42:30 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Jan 18 04:46:49 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <09010BC3D0D34D66BEB1F049EDD87AC8@dielangs.at> References: <11b37.480758ab.3c478a50@aol.com>, <09010BC3D0D34D66BEB1F049EDD87AC8@dielangs.at> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB3058C2@LRGHEXVS1.practice.lrgh.org> I have always allowed music to play at work usually left on a radio station since it will keep us updated on the day's information and leave the more offensive language out. I bought the department a system that will play cd and iPods'. The only rule is while playing our iPods', the songs with the strong language must be skipped. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Wednesday, January 18, 2012 5:12 AM To: Traczyk7@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Music in the Laboratory It would be fine, if there could be found a compromise. We have the radio on during cutting time, but it is quiet enough not to "overblow" work. And in the minutes nobody talks it is quite amousing to listen daily infos and music. And yes, also for me it would be a pain to listen to "hard" music at work all the time. On the other hand, a pathologist in our department loves classic music at the microscope. - that's also too much for me. ;) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Traczyk7@aol.com Gesendet: Mittwoch, 18. J?nner 2012 03:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang "F" word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales & service reps. Your ideas on this is very much appreciated. Dorothy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Karen.Heckford <@t> CHW.edu Wed Jan 18 07:07:57 2012 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Jan 18 07:08:07 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <11b37.480758ab.3c478a50@aol.com> References: <11b37.480758ab.3c478a50@aol.com> Message-ID: <3328693C43A557458850CC37CE16CD185B738871@chw-msg-829.chw.edu> I could not work with out music. Nothing like cutting to the rhythm of good music. I have it on all the time or I would go nuts and probably fall asleep. I set my radio to one radio station. I am the Chief Cook and bottle washer so no one to bother with the music. If someone wants to play hard core obscene music let them put it on their Ipod so not everyone can here it. I would make a rule that they have to be able to hear if someone is talking to them. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Tuesday, January 17, 2012 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang "F" word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales & service reps. Your ideas on this is very much appreciated. Dorothy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Wed Jan 18 07:29:48 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Jan 18 07:29:53 2012 Subject: [Histonet] K/L bone marrow Message-ID: Does anyone have a Kappa/Lambda bone marrow ISH protocol for use on the Ventana Benchmark XT? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From charchar999 <@t> gmail.com Wed Jan 18 08:15:23 2012 From: charchar999 <@t> gmail.com (Charity Wyatt) Date: Wed Jan 18 08:15:32 2012 Subject: [Histonet] Fwd: Rapid MART-1 procedure In-Reply-To: References: Message-ID: ---------- Forwarded message ---------- From: Charity Wyatt Date: Wed, Jan 18, 2012 at 9:01 AM Subject: Rapid MART-1 procedure To: "histonet@lists.utsouthwestern.edu" Hi All! I work for a busy Mohs surgeon that wants to start doing a rapid MART-1 (Melan-A) immunostain with our melanoma cases. Does anyone have any suggestions/protocols/vendor names to help me get started? I'm starting from the ground up here, we don't currently do any immuno stains. We will be doing the stains on frozen sections, and they will be done manually. Any tips would be greatly appreciated! Thanks! -- Charity Wyatt Mohs Histotechnologist Jacksonville Skin Cancer Center, P.A./ Michael E. Lutz, M.D. (904)737-0111 -- Charity Wyatt Mohs Histotechnologist Jacksonville Skin Cancer Center, P.A./ Michael E. Lutz, M.D. (904)737-0111 From christiegowan <@t> msn.com Wed Jan 18 08:20:44 2012 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Jan 18 08:20:52 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <11b37.480758ab.3c478a50@aol.com> References: <11b37.480758ab.3c478a50@aol.com> Message-ID: Hi All, Our lab is governed by hospital regulations that no headphones or earbuds be worn at anytime. This is considered a safety issue. We do have policies governing codes for behavior such as dress, hygiene, innappropriate computer use such as streaming music or social media but music is something they allow idividual departments to dictate. Our lab has decided to allow desk top radios to be played. I have a few techs that like to listen to talk radio or music so they each have their individual radios set so low that only they can hear it. I think if everyone had their own radio it would be insane but a couple is not too bad. If it were to become distracting or a nusance, I would ban them completely. We are not in a patient traffic area but we do get a lot of outside visitors walking thru such as Resident's interviewing or clinicians. One must always consider patient care first and foremost and if music is offensive to you because of language then it probably is offensive to others as well. I think you should never have to endure music that is offensive in anyway. Jazz makes me crazy. My question to you would be, do you have a policy that stating that music is allowed? Best of luck to you. Christie > From: Traczyk7@aol.com > Date: Tue, 17 Jan 2012 21:37:04 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Music in the Laboratory > > Greetings. > I would like to know what other histology laboratories allow for music > players while working. Do you have formal policies about music content or > volume? Do you allow lab space doors to remain closed to muffle the volume of > what is being played? Are headsets allowed? > I am a terrible judge of this because I personally prefer to work in a > quiet environment. I am trying to be open minded, as long as the work gets > done. However, one of the techs had a song playing today that I believe was > inappropriate for general listening in the lab. Am I just out of touch? > Is that dang "F" word just something I'm going to have to learn to accept? > Do you have a written policy? When/how/why was it implemented? > I should mention that it's a small private lab, with minimal patient > traffic. We do see our share of FedEx, UPS, sales & service reps. > Your ideas on this is very much appreciated. > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Jan 18 08:38:27 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jan 18 08:38:36 2012 Subject: [Histonet] Quenching auto fluorescence in human tissue In-Reply-To: <9DF797D618351549B984596F01A1FE1D0222F7D8@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D0222F7D8@murmx.mcri.edu.au> Message-ID: I have not used "Chicago Blue" (Inoticed the product is discontinued by Sigma Aldrich) for quenching, but have often used "Evans Blue" (synonym: Direct Blue 53; Empirical Formula: C34H24N6Na4O14S4 ;Molecular Weight: 960.81;CAS Number: 314-13-6) without any issue. Sigma Aldrich sells the Evans Blue is solution (>75% dye content). You might want to give the Evans Blue a try (product # E2129). William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 18 Jan 2012 16:11:51 +1100 > From: daniela.bodemer@mcri.edu.au > To: Histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Quenching auto fluorescence in human tissue > > Hi all, > > > > I have been experiencing trouble with my secondary antibody when doing > my immuno with human colon tissue. > > I use to use FITC 488 as my secondary antibody and changed over to > Alexa488 due to the FITC's manufacturing stop. > > The auto fluorescence of the human tissue is quenched with Chicago Blue > solution and the slides are mounted with Prolong Gold antifade reagent > to extend the life of the immuno slides. > > The trouble is, after a few days the fluorofors seem to 'detach' and > start floating around, not allowing reliable validation of the results. > This also happen if I use Mowiol as a mounting medium. > > Is the mounting medium reacting with Chicago Blue? Is there any > alternative to quench die auto fluorescence of the tissue? > > > > Thanks for your ideas. > > > > Daniela Bodemer > > Research Assistant > > Surgical Research, Infection and Immunity > > > > Murdoch Childrens Research Institute > > The Royal Children's Hospital > > Flemington Road Parkville Victoria 3052 Australia > > T 03 9936 6676 T (03 9936 6794) > > E daniela.bodemer@mcri.edu.au > > www.mcri.edu.au > > > > This e-mail and any attachments to it (the "Communication") are, unless > otherwise stated, confidential, may contain copyright material and is > for the use only of the intended recipient. If you receive the > Communication in error, please notify the sender immediately by return > e-mail, delete the Communication and the return e-mail, and do not read, > copy, retransmit or otherwise deal with it. Any views expressed in the > Communication are those of the individual sender only, unless expressly > stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 > 006 566 972 or any of its related entities. MCRI does not accept > liability in connection with the integrity of or errors in the > Communication, computer virus, data corruption, interference or delay > arising from or in respect of the Communication. > > P Please consider the environment before printing this email > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Jan 18 08:51:53 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jan 18 08:52:02 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: References: <11b37.480758ab.3c478a50@aol.com> Message-ID: I am lucky enough to work in a closed lab setting so we can play whatever, until the boss comes in (she can't concentrate with music on). If I couldn't listen to something while sectioning, I think I might die of boredom. But forcing other people to listen to your music is never good, even when you're with friends. If everyone doesn't like it, pick something that everyone does like. I suggest radiolab--it's a fun podcast where you can actually learn something new about science while enjoying yourself. Plus it it doesn't have politics! My favorite episode is Lost and Found, it's the first one I heard, and I love it. But I suggest you start with Oops, that one was amazing. http://www.radiolab.org/ Any other podcasts that are science related and fun? (No politics please!) Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Wed, Jan 18, 2012 at 9:20 AM, CHRISTIE GOWAN wrote: > > Hi All, > Our lab is governed by hospital regulations that no headphones or earbuds > be worn at anytime. This is considered a safety issue. We do have policies > governing codes for behavior such as dress, hygiene, innappropriate > computer use such as streaming music or social media but music is something > they allow idividual departments to dictate. Our lab has decided to allow > desk top radios to be played. I have a few techs that like to listen to > talk radio or music so they each have their individual radios set so low > that only they can hear it. I think if everyone had their own radio it > would be insane but a couple is not too bad. If it were to become > distracting or a nusance, I would ban them completely. We are not in a > patient traffic area but we do get a lot of outside visitors walking thru > such as Resident's interviewing or clinicians. One must always consider > patient care first and foremost and if music is offensive to you because of > language then it probably is offensive to others as well. I think you > should never have to endure music that is offensive in anyway. Jazz makes > me crazy. My question to you would be, do you have a policy that stating > that music is allowed? Best of luck to you. > Christie > > > > From: Traczyk7@aol.com > > Date: Tue, 17 Jan 2012 21:37:04 -0500 > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Music in the Laboratory > > > > Greetings. > > I would like to know what other histology laboratories allow for music > > players while working. Do you have formal policies about music content or > > volume? Do you allow lab space doors to remain closed to muffle the > volume of > > what is being played? Are headsets allowed? > > I am a terrible judge of this because I personally prefer to work in a > > quiet environment. I am trying to be open minded, as long as the work > gets > > done. However, one of the techs had a song playing today that I believe > was > > inappropriate for general listening in the lab. Am I just out of touch? > > Is that dang "F" word just something I'm going to have to learn to > accept? > > Do you have a written policy? When/how/why was it implemented? > > I should mention that it's a small private lab, with minimal patient > > traffic. We do see our share of FedEx, UPS, sales & service reps. > > Your ideas on this is very much appreciated. > > Dorothy > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Wed Jan 18 08:52:09 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 18 08:52:27 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: References: <11b37.480758ab.3c478a50@aol.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7DB1F@SMCMAIL01.somerset-healthcare.com> Regarding the offensive language, many institutions have policies that address the use of inappropriate language. You may find it in something like a "Workplace Harassment/Violence" policy. We too do not permit ear buds or headphones for the same reason. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Wednesday, January 18, 2012 9:21 AM To: traczyk7@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Music in the Laboratory Hi All, Our lab is governed by hospital regulations that no headphones or earbuds be worn at anytime. This is considered a safety issue. We do have policies governing codes for behavior such as dress, hygiene, innappropriate computer use such as streaming music or social media but music is something they allow idividual departments to dictate. Our lab has decided to allow desk top radios to be played. I have a few techs that like to listen to talk radio or music so they each have their individual radios set so low that only they can hear it. I think if everyone had their own radio it would be insane but a couple is not too bad. If it were to become distracting or a nusance, I would ban them completely. We are not in a patient traffic area but we do get a lot of outside visitors walking thru such as Resident's interviewing or clinicians. One must always consider patient care first and foremost and if music is offensive to you because of language then it probably is offensive to others as well. I think you should never have to endure music that is offensive in anyway. Jazz makes me crazy. My question to you would be, do you have a policy that stating that music is allowed? Best of luck to you. Christie > From: Traczyk7@aol.com > Date: Tue, 17 Jan 2012 21:37:04 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Music in the Laboratory > > Greetings. > I would like to know what other histology laboratories allow for music > players while working. Do you have formal policies about music content > or volume? Do you allow lab space doors to remain closed to muffle the > volume of what is being played? Are headsets allowed? > I am a terrible judge of this because I personally prefer to work in a > quiet environment. I am trying to be open minded, as long as the work > gets done. However, one of the techs had a song playing today that I > believe was inappropriate for general listening in the lab. Am I just out of touch? > Is that dang "F" word just something I'm going to have to learn to accept? > Do you have a written policy? When/how/why was it implemented? > I should mention that it's a small private lab, with minimal patient > traffic. We do see our share of FedEx, UPS, sales & service reps. > Your ideas on this is very much appreciated. > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From shive003 <@t> umn.edu Wed Jan 18 09:05:07 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jan 18 09:05:16 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7DB1F@SMCMAIL01.somerset-healthcare.com> References: <11b37.480758ab.3c478a50@aol.com> <3AD061FE740D464FAC7BF6B5CFB7570711F7DB1F@SMCMAIL01.somerset-healthcare.com> Message-ID: Our policy is that if a radio station is to be played in the lab, the choice has to be a unanimous one of everyone working in the lab, it cannot be talk radio (prone to political leanings), it has to play appropriate music for work setting, and it has to be turned down low enough to almost not be audible. Personal iPods with only ONE earbud used is preferable to a room radio station. Jan Shivers On Wed, Jan 18, 2012 at 8:52 AM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > Regarding the offensive language, many institutions have policies that > address the use of inappropriate language. You may find it in something > like a "Workplace Harassment/Violence" policy. We too do not permit ear > buds or headphones for the same reason. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN > Sent: Wednesday, January 18, 2012 9:21 AM > To: traczyk7@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Music in the Laboratory > > > Hi All, > Our lab is governed by hospital regulations that no headphones or earbuds > be worn at anytime. This is considered a safety issue. We do have policies > governing codes for behavior such as dress, hygiene, innappropriate > computer use such as streaming music or social media but music is something > they allow idividual departments to dictate. Our lab has decided to allow > desk top radios to be played. I have a few techs that like to listen to > talk radio or music so they each have their individual radios set so low > that only they can hear it. I think if everyone had their own radio it > would be insane but a couple is not too bad. If it were to become > distracting or a nusance, I would ban them completely. We are not in a > patient traffic area but we do get a lot of outside visitors walking thru > such as Resident's interviewing or clinicians. One must always consider > patient care first and foremost and if music is offensive to you because of > language then it probably is offensive to others as well. I think you > should never have to endure music that is offensive in anyway. Jazz makes > me crazy. My question to you would be, do you have a policy that stating > that music is allowed? Best of luck to you. > Christie > > > > From: Traczyk7@aol.com > > Date: Tue, 17 Jan 2012 21:37:04 -0500 > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Music in the Laboratory > > > > Greetings. > > I would like to know what other histology laboratories allow for music > > players while working. Do you have formal policies about music content > > or volume? Do you allow lab space doors to remain closed to muffle the > > volume of what is being played? Are headsets allowed? > > I am a terrible judge of this because I personally prefer to work in a > > quiet environment. I am trying to be open minded, as long as the work > > gets done. However, one of the techs had a song playing today that I > > believe was inappropriate for general listening in the lab. Am I just > out of touch? > > Is that dang "F" word just something I'm going to have to learn to > accept? > > Do you have a written policy? When/how/why was it implemented? > > I should mention that it's a small private lab, with minimal patient > > traffic. We do see our share of FedEx, UPS, sales & service reps. > > Your ideas on this is very much appreciated. > > Dorothy > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Wed Jan 18 09:12:40 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jan 18 09:12:59 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: References: <11b37.480758ab.3c478a50@aol.com> Message-ID: <5367DEE1-D7FB-48C4-8BB1-93B6A917264F@email.arizona.edu> Good Morning all, I have worked in labs where music was played and loved it. Music keeps you going all morning and even in the mid-afternoon when you hit that "slump" time. If it is not inappropriate music or loud enough to be distracting when someone comes in with a question or when the phone rings I don't think it is a problem. One of my pathologists always listened to conservative talk radio and turned me on to it so being as how I'm lucky to usually be the only one in my lab I've become a talk radio junkie when I'm not listening to a novel on my iPod. Andi (I love my lab!) > >> Greetings. >> I would like to know what other histology laboratories allow for music >> players while working. Do you have formal policies about music content or >> volume? Do you allow lab space doors to remain closed to muffle the >> volume of >> what is being played? Are headsets allowed? >> I am a terrible judge of this because I personally prefer to work in a >> quiet environment. I am trying to be open minded, as long as the work gets >> done. However, one of the techs had a song playing today that I believe >> was >> inappropriate for general listening in the lab. Am I just out of touch? >> Is that dang "F" word just something I'm going to have to learn to accept? >> Do you have a written policy? When/how/why was it implemented? >> I should mention that it's a small private lab, with minimal patient >> traffic. We do see our share of FedEx, UPS, sales & service reps. >> Your ideas on this is very much appreciated. >> Dorothy >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > Question: Are rhinos overweight unicorns? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TGoins <@t> mt.gov Wed Jan 18 09:16:28 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Jan 18 09:16:41 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <11b37.480758ab.3c478a50@aol.com> References: <11b37.480758ab.3c478a50@aol.com> Message-ID: I got a pair of Bose speakers for the lab - I like to listen to quality sound while I work and we bring in ipods, phones, whatever. But, everyone has the option of nixing any music selection at any time or opting for no music. Depends on the mood of the lab on any particular day. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Tuesday, January 17, 2012 7:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang "F" word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales & service reps. Your ideas on this is very much appreciated. Dorothy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sruby <@t> 4path.com Wed Jan 18 09:33:18 2012 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Wed Jan 18 09:33:27 2012 Subject: [Histonet] Replys to multiple items Message-ID: 1. Photo microscopy.....if it's just for documentation, you may just be able to get by with taking a photo thru your ocular. Try it by using a hand held digital camera with the focus set to "infinity" or "landscape mode". It's trial and error, but if it works....then that is a really inexpensive option. 2. Music in labs. I have always had music and encourage it. However, it must be music that ALL people can enjoy and has NO offensive material. If you can't agree....then headsets or nothing. BTW...if you use headsets, the policy should include that the volume must be such that the user MUST be able to hear and respond to spoken communication at all times. It's a safety issue. 3. Billing question. Inappropriate to answer. You may be in violation of federal law to discuss pricing. My only suggestion...look at the medicare reimbursement schedule which is public record. You can look it up on google. Dr. R. From tkngflght <@t> yahoo.com Wed Jan 18 11:35:42 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jan 18 11:35:49 2012 Subject: [Histonet] Dorothy's music question Message-ID: <1326908142.16599.YahooMailNeo@web39401.mail.mud.yahoo.com> Hi Dorothy- ? Yes, those who manage the lab have the right and responsibility to control the environment and make it conducive to good work practices. Please also consider the other side -- the techs who do work better with a little music (some of us?ADD-prone bench folks). ? ? If you're in a high-volume lab and have a bunch of smart techs, sometimes music keeps the 'drama' down.? Put a bunch of smart techs on a highly repetitive bench and we find ways to entertain ourselves!? Music can help by keeping a not-fully-occupied brain entertained.? By limiting the open area radio stations to those without cuss-words and making volume levels 'personal' or allowing one ear-bud (not two: you have to be able to hear the work around you!!) is one way.? Having a rotation of who gets to pick the radio station (taking turns) and having a universal policy is another.? Be sure to address those tasks that may be uber-repetitive but require high accuracy for which radios are a conflict such as dictation and specimen entry. ?Cutting off radios after they've been in place can be counter-productive. Another way is to ask your techs to come up with a policy that meets their wishes without compromising the needs of the work.? Sometimes they'll come up with answers?that surprise and really do addresss the?work related issues: one group came up with a common radio on during peak cutting times and off during transitional task times so those who like quiet got their time, too! ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org? From JMacDonald <@t> mtsac.edu Wed Jan 18 12:22:19 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 18 12:22:25 2012 Subject: [Histonet] HT/HTL Military Programs Message-ID: Is anyone aware of any military based HT/HTL programs in the US? Thank you, Jennifer MacDonald From brett_connolly <@t> merck.com Wed Jan 18 12:53:22 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jan 18 12:53:27 2012 Subject: [Histonet] HT/HTL Military Programs In-Reply-To: References: Message-ID: Jennifer - Maybe at the Fort Sam Houston Academy of Health Sciences ?? I would check their AMEDD website. Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, January 18, 2012 1:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT/HTL Military Programs Is anyone aware of any military based HT/HTL programs in the US? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From POWELL_SA <@t> mercer.edu Wed Jan 18 14:03:49 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Jan 18 14:03:59 2012 Subject: [Histonet] You are invited - GSH hosts Region III April 13-15th 2012 Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD75EC343@MERCERMAIL.MercerU.local> Hi Fellow Histotechnology Professionals, Students and Vendors, Please forgive me if this is duplicated, it bounced the first time. I wanted to Invite you to Georgia, the Georgia Society for Histotechnology will be hosting the NSH Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,. Registration is Friday from 10-12 and the workshops begin at 1 pm. The last workshops are on Sunday morning. To view the complete program go to http://www.histosearch.com/gsh/symposium.html and print your copy today. Make your reservations soon and plan to attend, bring the family for a vacation, Callaway Gardens is a wonderful family experience. Register early for the fantastic workshops GSH has lined up; earn your CEUs. Payment is easy with PayPal. You do not have to have an account with them to use this service and it is secure. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here. Making reservations with a credit card will hold your room, they will not apply the rate until you actually check in. Call now 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program also. ***VENDORS: Vendors can print their registration form from the website to mail in and can make payment by going to www.PayPal.com and send payment to the email address on the registration form. There will be a Vendor Reception on Friday night, Awards Luncheon on Saturday, and a special dinner event Saturday night. See the details in the program here http://www.histosearch.com/gsh/symposium.html. Please contact our Vice President and Exhibit Liaison, Wanda Simons at wandrous@att.net if you have questions or concerns. If you have any questions please contact Wanda Simons, Mike Ayers or myself. Please pass this invitation to any who may not get this email. Come to Georgia and experience Histotechnology - Southern Style Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From tony.henwood <@t> health.nsw.gov.au Wed Jan 18 15:50:06 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jan 18 15:50:24 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7DB1F@SMCMAIL01.somerset-healthcare.com> References: <11b37.480758ab.3c478a50@aol.com> <3AD061FE740D464FAC7BF6B5CFB7570711F7DB1F@SMCMAIL01.somerset-healthcare.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A154BC@xmdb02.nch.kids> I suppose naughty words have appeared in songs for years (Chuck Berry's "My Ding-a-ling" must have been a little riskie - or is it just my FF (formalin fixed) mind!) We often have the radio on and one day the staff, especially the young ones, thought I was "quite hip!" as I was bopping along to a song that seemed to contain naughty words. Fortunately I had no idea what the words to the song were (I needed sub-titles) - and that seems to be the case with most of them now. My hearin is probably shot from too much Deep Purple, Black Sabbath and ACDC anyway!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, 19 January 2012 1:52 AM To: 'CHRISTIE GOWAN'; traczyk7@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Music in the Laboratory Regarding the offensive language, many institutions have policies that address the use of inappropriate language. You may find it in something like a "Workplace Harassment/Violence" policy. We too do not permit ear buds or headphones for the same reason. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Wednesday, January 18, 2012 9:21 AM To: traczyk7@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Music in the Laboratory Hi All, Our lab is governed by hospital regulations that no headphones or earbuds be worn at anytime. This is considered a safety issue. We do have policies governing codes for behavior such as dress, hygiene, innappropriate computer use such as streaming music or social media but music is something they allow idividual departments to dictate. Our lab has decided to allow desk top radios to be played. I have a few techs that like to listen to talk radio or music so they each have their individual radios set so low that only they can hear it. I think if everyone had their own radio it would be insane but a couple is not too bad. If it were to become distracting or a nusance, I would ban them completely. We are not in a patient traffic area but we do get a lot of outside visitors walking thru such as Resident's interviewing or clinicians. One must always consider patient care first and foremost and if music is offensive to you because of language then it probably is offensive to others as well. I think you should never have to endure music that is offensive in anyway. Jazz makes me crazy. My question to you would be, do you have a policy that stating that music is allowed? Best of luck to you. Christie > From: Traczyk7@aol.com > Date: Tue, 17 Jan 2012 21:37:04 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Music in the Laboratory > > Greetings. > I would like to know what other histology laboratories allow for music > players while working. Do you have formal policies about music content > or volume? Do you allow lab space doors to remain closed to muffle the > volume of what is being played? Are headsets allowed? > I am a terrible judge of this because I personally prefer to work in a > quiet environment. I am trying to be open minded, as long as the work > gets done. However, one of the techs had a song playing today that I > believe was inappropriate for general listening in the lab. Am I just out of touch? > Is that dang "F" word just something I'm going to have to learn to accept? > Do you have a written policy? When/how/why was it implemented? > I should mention that it's a small private lab, with minimal patient > traffic. We do see our share of FedEx, UPS, sales & service reps. > Your ideas on this is very much appreciated. > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From MYang <@t> emc.org Wed Jan 18 18:24:02 2012 From: MYang <@t> emc.org (Yang, Mari) Date: Wed Jan 18 18:24:07 2012 Subject: [Histonet] Part Time Cytotechnologist, Eisenhower Medical Center Message-ID: <3340FC2AE9CFEE4E9D001D077700C6A2253ED6A5@NT106.info.sys> Greetings Histoland! I would like to share with everyone an exciting position that has become available at Eisenhower Medical Center located in Rancho Mirage, CA. The job opening is for a part time Cytotechnologist screening non-gyn only. Please share with anyone whom may be interested. Applications are processed through www.emc.org . ====== Part Time Cytotechnologist Job Objective: Performs examinations of non-gynecological cytology specimens by screening for abnormalities. Assists with fine needle aspirations to include on-site preparation, staining, and rapid evaluation of collected material. Reviews and signs out cytology cases with the appropriate pathologist or Cytology Supervisor. Performs clerical and computer tasks as assigned. Education Preferred: BS and one year of formal, approved training Licensure/Certification Required: CA state license Experience Preferred: 3 years as a Cytotech in the past 10 years Thanks, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From one_angel_secret <@t> yahoo.com Wed Jan 18 18:52:55 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Jan 18 18:53:07 2012 Subject: [Histonet] Music in the Laboratory In-Reply-To: <5367DEE1-D7FB-48C4-8BB1-93B6A917264F@email.arizona.edu> References: <11b37.480758ab.3c478a50@aol.com> <5367DEE1-D7FB-48C4-8BB1-93B6A917264F@email.arizona.edu> Message-ID: <650777BA-A14B-4D48-9AA2-1099748F062E@yahoo.com> I agree with what you say Andi. Music is a great stress reliever as well. Ive always let the group decide if they are all willing to tolerate other kinds of music to be fair. I always ask them to try to keep it clean. Don't want to offend With that being said. I myself have had to tone it down because I love me some disco music:). Yes, ring my bell and shake your groove thing lol Without further silliness. Music is great in the lab if you can get the people to agree on compromise.if you have someone you know is very strict and you know Katy perrys I kissed a girl song is going to send them over the edge. Don't do it. Let them use headphones. Because you wouldn't want someone claiming harassment. Kim D On Jan 18, 2012, at 10:12 AM, "Grantham, Andrea L - (algranth)" wrote: > Good Morning all, > I have worked in labs where music was played and loved it. Music keeps you going all morning and even in the mid-afternoon when you hit that "slump" time. If it is not inappropriate music or loud enough to be distracting when someone comes in with a question or when the phone rings I don't think it is a problem. > One of my pathologists always listened to conservative talk radio and turned me on to it so being as how I'm lucky to usually be the only one in my lab I've become a talk radio junkie when I'm not listening to a novel on my iPod. > > Andi > (I love my lab!) > > > >> >>> Greetings. >>> I would like to know what other histology laboratories allow for music >>> players while working. Do you have formal policies about music content or >>> volume? Do you allow lab space doors to remain closed to muffle the >>> volume of >>> what is being played? Are headsets allowed? >>> I am a terrible judge of this because I personally prefer to work in a >>> quiet environment. I am trying to be open minded, as long as the work gets >>> done. However, one of the techs had a song playing today that I believe >>> was >>> inappropriate for general listening in the lab. Am I just out of touch? >>> Is that dang "F" word just something I'm going to have to learn to accept? >>> Do you have a written policy? When/how/why was it implemented? >>> I should mention that it's a small private lab, with minimal patient >>> traffic. We do see our share of FedEx, UPS, sales & service reps. >>> Your ideas on this is very much appreciated. >>> Dorothy >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> Louise Renton >> Bone Research Unit >> University of the Witwatersrand >> Johannesburg >> South Africa >> +27 11 717 2298 (tel & fax) >> 073 5574456 (emergencies only) >> Question: Are rhinos overweight unicorns? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Jan 18 19:02:49 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jan 18 19:02:54 2012 Subject: [Histonet] Music in the lab Message-ID: Hi, I am in the same boat as those that would go nuts without music. I have noticed that I cut a *heck* of a lot faster with music than without. It's a rhythm thing, I can't really explain it. I really can sympathize with those that can't tolerate certain types of music though. Having one tech happy and another with a headache is not helpful or productive. Not everyone will agree on good music though, so you need to try to be versatile and share the air. For open air music (not personal music with headphones) I like to stick to music that would be played on popular radio stations. That way the FCC can determine what's appropriate. Admittedly that's a bit risky if you have been following the court case about indecency fines, but that leads us to politics and that's likely to draw fire as well. Neutrality is best, NPR works well there. Radiolab was a great suggestion! Thanks for that. If anyone has other good podcasts, please share. I like headphones, but if it interferes with communication or productivity it's gotta go. I don't like repeating myself and it is just rude to everyone around you. Amos From H.J.G.vandeKant <@t> uu.nl Thu Jan 19 01:10:11 2012 From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk)) Date: Thu Jan 19 01:10:20 2012 Subject: [Histonet] remove my email addres Message-ID: <8A014D954ADDDD44AC39749D8EB677C9CE2B86@ICTSC-W-S205.soliscom.uu.nl> Dear Histonet, Although I truly appreciate all the members for helping me in the past, please remove my email address from the list. Thank you. Henk van de Kant From LSebree <@t> uwhealth.org Thu Jan 19 08:25:42 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jan 19 08:25:48 2012 Subject: [Histonet] slide file storage to dry slides Message-ID: Good morning all, We've recently switched from film coverslipping back to glass and therefore need to thoroughly dry our slides before permanent filing. I recall, in my first histology job....30 + years ago, that we used metal stacking slide files that you could put an insert into the drawers that looked like a non-stretchy spring. The wires of this "spring" held the slides apart to dry, then they could be filed without the "spring" when they were completely dry. Anyone know if that product still exists? Or does anyone have a better solution for drying slides while still keeping them in order? Thanks for the assist, Linda From JWeems <@t> sjha.org Thu Jan 19 08:58:03 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 19 08:58:14 2012 Subject: [Histonet] RE: slide file storage to dry slides In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164084584CF7E@CHEXCMS10.one.ads.che.org> We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050 102 - that doesn't need extra drying. File the next day with no sticking.. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, January 19, 2012 09:26 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide file storage to dry slides Good morning all, We've recently switched from film coverslipping back to glass and therefore need to thoroughly dry our slides before permanent filing. I recall, in my first histology job....30 + years ago, that we used metal stacking slide files that you could put an insert into the drawers that looked like a non-stretchy spring. The wires of this "spring" held the slides apart to dry, then they could be filed without the "spring" when they were completely dry. Anyone know if that product still exists? Or does anyone have a better solution for drying slides while still keeping them in order? Thanks for the assist, Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From DKBoyd <@t> chs.net Thu Jan 19 09:25:47 2012 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Jan 19 09:26:00 2012 Subject: [Histonet] RE: slide file storage to dry slides In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164084584CF7E@CHEXCMS10.one.ads.che.org> Message-ID: Joyce, Interesting! What methodology are using to remove the coverslip and with what difficulty? I may be interested in changing to this medium. Are you using this same medium with Non-gyn Cytology and have you had any bleeding problems? Also we do not use Xylene. We use a substitute. Thanks! Debbie Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/19/2012 09:58 AM To Sebree Linda A , "Histonet@lists.utsouthwestern.edu" cc Subject [Histonet] RE: slide file storage to dry slides We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050 102 - that doesn't need extra drying. File the next day with no sticking.. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, January 19, 2012 09:26 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide file storage to dry slides Good morning all, We've recently switched from film coverslipping back to glass and therefore need to thoroughly dry our slides before permanent filing. I recall, in my first histology job....30 + years ago, that we used metal stacking slide files that you could put an insert into the drawers that looked like a non-stretchy spring. The wires of this "spring" held the slides apart to dry, then they could be filed without the "spring" when they were completely dry. Anyone know if that product still exists? Or does anyone have a better solution for drying slides while still keeping them in order? Thanks for the assist, Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From relia1 <@t> earthlink.net Thu Jan 19 09:32:36 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 19 09:32:46 2012 Subject: [Histonet] RELIA Histology Careers Bulletin 1-19-2012 Any New Years Resolutions? Message-ID: <4979BB55C201476FB8E631F896AD96E6@ownerf1abaad51> Hi Histonetters!! Did you make a New Year?s Resolution? How is it going so far? Of course I resolved that I will try to eat right and exercise and I am trying. But my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2011 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get higher salaries, more desirable shifts and better benefits. In 2012 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately the quality of their lives. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here is a list of my current openings: HISTOLOGY MANAGEMENT * Histology Supervisor/Nights - Miami, FL * Regional Histology Supervisor - Portland, OR * Histology Manager - Suffern, NY HISTO TECHS * Grossing Histotech ? Chattanooga, TN* * Histology Tech ? Salem, VA * Histology Tech - Long Island, NY * Night Shift Grossing Histotech ? Wallingford, CT * Histotech ? Milwaukee, WI * Histotech ? Culver City, CA* * Histotech ? Pismo Beach, CA* * IHC Specialist - Valencia, CA * Grossing Histotech/PA - Charlotte, NC * Path Assistant - Lancaster, PA Of course I can?t put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don?t worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. Thanks again and I hope to hear from you soon, Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From FLiang <@t> wellstatoc.com Thu Jan 19 09:40:19 2012 From: FLiang <@t> wellstatoc.com (Liang, Frank) Date: Thu Jan 19 09:40:29 2012 Subject: [Histonet] RE: paraffin embedding mold for rat eye In-Reply-To: <4979BB55C201476FB8E631F896AD96E6@ownerf1abaad51> References: <4979BB55C201476FB8E631F896AD96E6@ownerf1abaad51> Message-ID: Hi, everyone, Any suggestions for the right size of mold to embed rat eyes in paraffin? Where we can get them? The molds we currently use are good for mouse eyes. Thanks, Frank -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Thursday, January 19, 2012 10:33 AM To: 'Histonet' Subject: [Histonet] RELIA Histology Careers Bulletin 1-19-2012 Any New Years Resolutions? Hi Histonetters!! Did you make a New Year's Resolution? How is it going so far? Of course I resolved that I will try to eat right and exercise and I am trying. But... my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2011 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get higher salaries, more desirable shifts and better benefits. In 2012 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately the quality of their lives. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here is a list of my current openings: HISTOLOGY MANAGEMENT * Histology Supervisor/Nights - Miami, FL * Regional Histology Supervisor - Portland, OR * Histology Manager - Suffern, NY HISTO TECHS * Grossing Histotech - Chattanooga, TN* * Histology Tech - Salem, VA * Histology Tech - Long Island, NY * Night Shift Grossing Histotech - Wallingford, CT * Histotech - Milwaukee, WI * Histotech - Culver City, CA* * Histotech - Pismo Beach, CA* * IHC Specialist - Valencia, CA * Grossing Histotech/PA - Charlotte, NC * Path Assistant - Lancaster, PA Of course I can't put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don't worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. Thanks again and I hope to hear from you soon, Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jan 19 09:51:08 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 19 09:51:16 2012 Subject: [Histonet] RE: paraffin embedding mold for rat eye In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC557C@SBS2K8.premierlab.local> It depends upon how you are going to section the eye, we place trimmed rat eyes for retinopathy work in the small square base molds 15mm x 15mm . If we need to section past the optic nerve and also collect sections on the edge of eye we embed in the deep molds, that way we don't have to re embed the samples. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liang, Frank Sent: Thursday, January 19, 2012 8:40 AM To: 'Pam Barker'; 'Histonet' Subject: [Histonet] RE: paraffin embedding mold for rat eye Hi, everyone, Any suggestions for the right size of mold to embed rat eyes in paraffin? Where we can get them? The molds we currently use are good for mouse eyes. Thanks, Frank -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Thursday, January 19, 2012 10:33 AM To: 'Histonet' Subject: [Histonet] RELIA Histology Careers Bulletin 1-19-2012 Any New Years Resolutions? Hi Histonetters!! Did you make a New Year's Resolution? How is it going so far? Of course I resolved that I will try to eat right and exercise and I am trying. But... my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2011 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get higher salaries, more desirable shifts and better benefits. In 2012 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately the quality of their lives. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here is a list of my current openings: HISTOLOGY MANAGEMENT * Histology Supervisor/Nights - Miami, FL * Regional Histology Supervisor - Portland, OR * Histology Manager - Suffern, NY HISTO TECHS * Grossing Histotech - Chattanooga, TN* * Histology Tech - Salem, VA * Histology Tech - Long Island, NY * Night Shift Grossing Histotech - Wallingford, CT * Histotech - Milwaukee, WI * Histotech - Culver City, CA* * Histotech - Pismo Beach, CA* * IHC Specialist - Valencia, CA * Grossing Histotech/PA - Charlotte, NC * Path Assistant - Lancaster, PA Of course I can't put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don't worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. Thanks again and I hope to hear from you soon, Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Jan 19 09:51:27 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Jan 19 09:51:40 2012 Subject: [Histonet] RE: paraffin embedding mold for rat eye In-Reply-To: References: <4979BB55C201476FB8E631F896AD96E6@ownerf1abaad51> Message-ID: <270B0381-D2AC-4C05-91CF-61D66FAE8936@email.arizona.edu> You could contact Simport. A few years ago I got some deeper disposable molds from them and they work great with tissues that are too high for the regular molds (like the Tissue-tek molds). The only problem is that they only came in the large width and you waste a lot of paraffin. Another thought is Polysciences Peel Away molds. Worse case you could do the old fashioned thing and make a paper mold the size and depth that you need. Andi On Jan 19, 2012, at 8:40 AM, Liang, Frank wrote: > Hi, everyone, > > Any suggestions for the right size of mold to embed rat eyes in paraffin? Where we can get them? The molds we currently use are good for mouse eyes. > > Thanks, > > Frank > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker > Sent: Thursday, January 19, 2012 10:33 AM > To: 'Histonet' > Subject: [Histonet] RELIA Histology Careers Bulletin 1-19-2012 Any New Years Resolutions? > > Hi Histonetters!! > > Did you make a New Year's Resolution? How is it going so far? Of course I > resolved that I will try to eat right and exercise and I am trying. > > But... my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. > > In 2011 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get higher salaries, more desirable shifts and better benefits. > > In 2012 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately the quality of their lives. > > I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. > > Here is a list of my current openings: > > HISTOLOGY MANAGEMENT > > * Histology Supervisor/Nights - Miami, FL > > * Regional Histology Supervisor - Portland, OR > > * Histology Manager - Suffern, NY > > HISTO TECHS > > * Grossing Histotech - Chattanooga, TN* > > * Histology Tech - Salem, VA > > * Histology Tech - Long Island, NY > > * Night Shift Grossing Histotech - Wallingford, CT > > * Histotech - Milwaukee, WI > > * Histotech - Culver City, CA* > > * Histotech - Pismo Beach, CA* > > * IHC Specialist - Valencia, CA > > * Grossing Histotech/PA - Charlotte, NC > * Path Assistant - Lancaster, PA > > Of course I can't put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. > > Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don't worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. > > So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. > > There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. > > Thanks again and I hope to hear from you soon, Thanks-Pam > > Thank You! > > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > www.facebook.comPamBarkerRELIA > www.linkedin.com/reliasolutions > www.myspace.com/pamatrelia > www.twitter.com/pamatrelia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Thu Jan 19 10:13:17 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 19 10:13:27 2012 Subject: AW: [Histonet] Music in the Laboratory In-Reply-To: <650777BA-A14B-4D48-9AA2-1099748F062E@yahoo.com> References: <11b37.480758ab.3c478a50@aol.com><5367DEE1-D7FB-48C4-8BB1-93B6A917264F@email.arizona.edu> <650777BA-A14B-4D48-9AA2-1099748F062E@yahoo.com> Message-ID: In German-speaking Austria we have the advantage not to understand every word of the songs. So it can happen, that we sing out loud lyrics, that we never took in mouth in our mothertounge. Sometimes funny, and sometimes much less stress. xD Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kim Donadio Gesendet: Donnerstag, 19. J?nner 2012 01:53 An: Grantham, Andrea L - (algranth) Cc: Histonet Betreff: Re: [Histonet] Music in the Laboratory I agree with what you say Andi. Music is a great stress reliever as well. Ive always let the group decide if they are all willing to tolerate other kinds of music to be fair. I always ask them to try to keep it clean. Don't want to offend With that being said. I myself have had to tone it down because I love me some disco music:). Yes, ring my bell and shake your groove thing lol Without further silliness. Music is great in the lab if you can get the people to agree on compromise.if you have someone you know is very strict and you know Katy perrys I kissed a girl song is going to send them over the edge. Don't do it. Let them use headphones. Because you wouldn't want someone claiming harassment. Kim D On Jan 18, 2012, at 10:12 AM, "Grantham, Andrea L - (algranth)" wrote: > Good Morning all, > I have worked in labs where music was played and loved it. Music keeps you going all morning and even in the mid-afternoon when you hit that "slump" time. If it is not inappropriate music or loud enough to be distracting when someone comes in with a question or when the phone rings I don't think it is a problem. > One of my pathologists always listened to conservative talk radio and turned me on to it so being as how I'm lucky to usually be the only one in my lab I've become a talk radio junkie when I'm not listening to a novel on my iPod. > > Andi > (I love my lab!) > > > >> >>> Greetings. >>> I would like to know what other histology laboratories allow for music >>> players while working. Do you have formal policies about music content or >>> volume? Do you allow lab space doors to remain closed to muffle the >>> volume of >>> what is being played? Are headsets allowed? >>> I am a terrible judge of this because I personally prefer to work in a >>> quiet environment. I am trying to be open minded, as long as the work gets >>> done. However, one of the techs had a song playing today that I believe >>> was >>> inappropriate for general listening in the lab. Am I just out of touch? >>> Is that dang "F" word just something I'm going to have to learn to accept? >>> Do you have a written policy? When/how/why was it implemented? >>> I should mention that it's a small private lab, with minimal patient >>> traffic. We do see our share of FedEx, UPS, sales & service reps. >>> Your ideas on this is very much appreciated. >>> Dorothy >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> Louise Renton >> Bone Research Unit >> University of the Witwatersrand >> Johannesburg >> South Africa >> +27 11 717 2298 (tel & fax) >> 073 5574456 (emergencies only) >> Question: Are rhinos overweight unicorns? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihisto <@t> hotmail.com Thu Jan 19 10:13:25 2012 From: brandihisto <@t> hotmail.com (Brandi Farris) Date: Thu Jan 19 10:13:31 2012 Subject: [Histonet] Meditech user and cassette/slide labeler Message-ID: Hi! Our lab is currently using Meditech and we are wanting to start looking at automated cassette and slide labelers. Is anyone know of a system that interfaces? Any experience with brands that they would like to pass along? Vendors are also welcome to contact me. Thank you, Brandi Farris Capital Region Medical Center Jefferson City, MO 65101 From brandihisto <@t> hotmail.com Thu Jan 19 10:18:21 2012 From: brandihisto <@t> hotmail.com (Brandi Farris) Date: Thu Jan 19 10:18:30 2012 Subject: [Histonet] Xylene Message-ID: Hi! Our lab is using xylene substitute (Clear Rite 3), but we recently purchased an automated coverslipper and would prefer to keep a small quanity (32 ounces) of xylene on hand for cleaning the machine and such. Does anyone know of any regulation with keeping such a small quanity. Are we required to monitor xylene fumes? Are we required to track disposal, ect? I purchased this amount at the local hardware store. Thank you, Brandi Farris Capital Region Medical Center Jefferson City, MO 65101 From katelin09htl <@t> gmail.com Thu Jan 19 11:58:16 2012 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Thu Jan 19 11:58:21 2012 Subject: [Histonet] Part Time Histologist Opening in Tualatin, OR Message-ID: Part time certified HT or HTL wanted for busy GI practice in Tualatin, Oregon. Candidate must be ASCP certified and CLIA certified to perform gross dissection. GI experience preferred. This is a M-F position with no weekends , holidays or call. Includes Full Medical & Dental benefits plus a 401K program. Interested applicants should forward their resume to gbacon@gispecialists.org -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 From mdpraet <@t> gmail.com Thu Jan 19 12:29:20 2012 From: mdpraet <@t> gmail.com (mequita praet) Date: Thu Jan 19 12:29:27 2012 Subject: [Histonet] automatic stainer/coverslipper combos Message-ID: Hi All, I am interested in your opinions of the automatic stainer/coverslipper combos. Do some companies still make them as one unit or just compatible? Would you please give me an idea of which ones seem to work the best and most reliably? Thank you, Mequita Praet From JWeems <@t> sjha.org Thu Jan 19 12:36:49 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 19 12:37:02 2012 Subject: [Histonet] automatic stainer/coverslipper combos In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164084584D048@CHEXCMS10.one.ads.che.org> We have used the Leica system successfully for a couple of years. A few glitches, but mostly good experience. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mequita praet Sent: Thursday, January 19, 2012 13:29 To: histonet Subject: [Histonet] automatic stainer/coverslipper combos Hi All, I am interested in your opinions of the automatic stainer/coverslipper combos. Do some companies still make them as one unit or just compatible? Would you please give me an idea of which ones seem to work the best and most reliably? Thank you, Mequita Praet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From katelin09htl <@t> gmail.com Thu Jan 19 12:58:45 2012 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Thu Jan 19 12:58:49 2012 Subject: [Histonet] automatic stainer/coverslipper combos In-Reply-To: References: Message-ID: We have the Sakura Tissue-Tek Prisma with attached tape coverslipper and I love it. I had so many problems with the Leica (which can be attached to it's coverslipper but mine wasn't) I would never go back. Feel free to email me offline with any specific questions. Katelin -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Thu, Jan 19, 2012 at 10:29 AM, mequita praet wrote: > Hi All, > I am interested in your opinions of the automatic stainer/coverslipper > combos. Do some companies still make them as one unit or just compatible? > Would you please give me an idea of which ones seem to work the best and > most reliably? > Thank you, > Mequita Praet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From loftonjt <@t> holycrosshealth.org Thu Jan 19 13:19:24 2012 From: loftonjt <@t> holycrosshealth.org (Jimmy Lofton) Date: Thu Jan 19 13:19:35 2012 Subject: [Histonet] automatic stainer/coverslipper combos In-Reply-To: References: Message-ID: <4F18266C02000056000059BE@nodcdmg2.no.trinity-health.org> Any concerns with the tape coverslipper? Occassionally we have had slides not coverslipped or skipped. Once it leaves our area, it looks fine, but later on some of the slides have dry spots but the coverslip looks intact. We also have the coverslipper at #4 for the flow of xylene. Thanks, Jimmy Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) loftonjt@holycrosshealth.org >>> Katelin Lester 01/19/2012 1:58 PM >>> We have the Sakura Tissue-Tek Prisma with attached tape coverslipper and I love it. I had so many problems with the Leica (which can be attached to it's coverslipper but mine wasn't) I would never go back. Feel free to email me offline with any specific questions. Katelin -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Thu, Jan 19, 2012 at 10:29 AM, mequita praet wrote: > Hi All, > I am interested in your opinions of the automatic stainer/coverslipper > combos. Do some companies still make them as one unit or just compatible? > Would you please give me an idea of which ones seem to work the best and > most reliably? > Thank you, > Mequita Praet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Trinity Health MailGate made the following annotations --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. --------------------------------------------------------------------- From dhewitt <@t> hvhs.org Thu Jan 19 13:22:15 2012 From: dhewitt <@t> hvhs.org (DANIEL HEWITT) Date: Thu Jan 19 13:22:24 2012 Subject: [Histonet] automatic stainer/coverslipper combos In-Reply-To: References: Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B01693FAF@MX-HVB-02.hvhs.org> Sakura has come out with a new coverslipper which seems to work well, we are trying it at one of our labs. In our lab we are currently using the leica and all I can say is it has good days and really bad days, needs lots of TLC. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katelin Lester Sent: Thursday, January 19, 2012 1:59 PM To: mequita praet Cc: histonet Subject: Re: [Histonet] automatic stainer/coverslipper combos We have the Sakura Tissue-Tek Prisma with attached tape coverslipper and I love it. I had so many problems with the Leica (which can be attached to it's coverslipper but mine wasn't) I would never go back. Feel free to email me offline with any specific questions. Katelin -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Thu, Jan 19, 2012 at 10:29 AM, mequita praet wrote: > Hi All, > I am interested in your opinions of the automatic stainer/coverslipper > combos. Do some companies still make them as one unit or just compatible? > Would you please give me an idea of which ones seem to work the best and > most reliably? > Thank you, > Mequita Praet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Jan 19 13:24:08 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Jan 19 13:24:13 2012 Subject: [Histonet] automatic stainer/coverslipper combos In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5D4E@PHSXMB30.partners.org> We have the Leica Autostainer XL and companion coverslipper CV5030 (glass coverslips) and like it a lot. We use the systems separately (space issue). We have had no problems with either. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mequita praet Sent: Thursday, January 19, 2012 1:29 PM To: histonet Subject: [Histonet] automatic stainer/coverslipper combos Hi All, I am interested in your opinions of the automatic stainer/coverslipper combos. Do some companies still make them as one unit or just compatible? Would you please give me an idea of which ones seem to work the best and most reliably? Thank you, Mequita Praet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From MSHERWOOD <@t> PARTNERS.ORG Thu Jan 19 13:26:00 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Jan 19 13:26:05 2012 Subject: [Histonet] automatic stainer/coverslipper combos In-Reply-To: <7DDB5AB36CBC574D8D680806E7BBE58B01693FAF@MX-HVB-02.hvhs.org> References: <7DDB5AB36CBC574D8D680806E7BBE58B01693FAF@MX-HVB-02.hvhs.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5D4F@PHSXMB30.partners.org> I would like to add an addendum to my prior email. We are a research lab and don't have the volume of a clinical histology lab (run roughly 6 racks or 30 slides each/day). Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, January 19, 2012 2:22 PM To: Katelin Lester; mequita praet Cc: histonet Subject: RE: [Histonet] automatic stainer/coverslipper combos Sakura has come out with a new coverslipper which seems to work well, we are trying it at one of our labs. In our lab we are currently using the leica and all I can say is it has good days and really bad days, needs lots of TLC. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katelin Lester Sent: Thursday, January 19, 2012 1:59 PM To: mequita praet Cc: histonet Subject: Re: [Histonet] automatic stainer/coverslipper combos We have the Sakura Tissue-Tek Prisma with attached tape coverslipper and I love it. I had so many problems with the Leica (which can be attached to it's coverslipper but mine wasn't) I would never go back. Feel free to email me offline with any specific questions. Katelin -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Thu, Jan 19, 2012 at 10:29 AM, mequita praet wrote: > Hi All, > I am interested in your opinions of the automatic stainer/coverslipper > combos. Do some companies still make them as one unit or just compatible? > Would you please give me an idea of which ones seem to work the best and > most reliably? > Thank you, > Mequita Praet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From member <@t> linkedin.com Thu Jan 19 14:09:35 2012 From: member <@t> linkedin.com (Bader Siddiki via LinkedIn) Date: Thu Jan 19 14:09:39 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1108467145.22640657.1327003775464.JavaMail.app@ela4-app0128.prod> LinkedIn ------------ Bader Siddiki requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Bader Accept invitation from Bader Siddiki http://www.linkedin.com/e/yvpgd1-gxm7sa8y-5z/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I212940440_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYMd3gMd3AOcj99bTlfkA9mlkVvbPoMdzkPdjkUdjkLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=0e1VA57-BVul41 View invitation from Bader Siddiki http://www.linkedin.com/e/yvpgd1-gxm7sa8y-5z/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I212940440_13/3cNnP0Qd30Qej8NcAALqnpPbOYWrSlI/svi/?hs=false&tok=0Nno1M-mxVul41 ------------------------------------------ Why might connecting with Bader Siddiki be a good idea? Bader Siddiki's connections could be useful to you: After accepting Bader Siddiki's invitation, check Bader Siddiki's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2011, LinkedIn Corporation From alisha <@t> ka-recruiting.com Thu Jan 19 16:01:51 2012 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Jan 19 15:58:03 2012 Subject: [Histonet] Histology Job Opportunities Message-ID: <2098516090.1327010511645.JavaMail.cfservice@sl4app3> Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histology Job Opportunities (HT or HTL): 1. Maine - Histotech 2. Indiana - Pathology Manager 3. NY - Westchester - Lead Histotech 4. NC - Histotech 3rd shift 5. FL - Histotech 6. OH - Histotech 7. NJ - Histotech 8. PA - Histotech and Lead Histotech 9. Western NY - Histotech 10. New York City - Histology Supervisor from Commerical Laboratory 11. Southern CA - Histotech 12. OK - Histotech 13. MN - Histotech 14. NC - Histology Supervisor 15. IN - Histotech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From jerry.santiago <@t> bellsouth.net Thu Jan 19 22:59:54 2012 From: jerry.santiago <@t> bellsouth.net (Jerry Santiago) Date: Thu Jan 19 22:59:59 2012 Subject: [Histonet] Florida Society for Histotechnology-Call for Abstracts Message-ID: <1327035594.33142.YahooMailRC@web181716.mail.ne1.yahoo.com> The Florida Society for Histotechnology is preparing for their Spring Annual Meeting. The meeting will be held at the Grand Hyatt Tampa Bay, May 17-20, 2012.We are calling for abstracts. If you are interested in presenting in our meeting, please go to www.fshgroup.com and download the abstract submission form. Meeting program will be made available in March. If you have any questions, please feel free to contact Jerry Santiago at W:904-766-6528 or C: 904-505-9989 Jerry Santiago, MSEd, HTL(ASCP)QIHC Florida State College at Jacksonville Histotechnology Program Assistant Professor/HT Program Director 4501 Capper Road Jacksonville, FL 32209 Tel: 904-766-6528 Fax: 904-766-5573 E-mail: jsantiag@fscj.edu From michelecarr10 <@t> yahoo.com Fri Jan 20 05:47:17 2012 From: michelecarr10 <@t> yahoo.com (Michele Email) Date: Fri Jan 20 05:47:21 2012 Subject: [Histonet] Tuberculosis positive tissue Message-ID: <99606419-54C4-4389-B38C-59DDB5E7D5E9@yahoo.com> Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated. Thank you Michele Carr Sent from my iPad From one_angel_secret <@t> yahoo.com Fri Jan 20 09:06:14 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jan 20 09:07:28 2012 Subject: [Histonet] Tuberculosis positive tissue In-Reply-To: <99606419-54C4-4389-B38C-59DDB5E7D5E9@yahoo.com> References: <99606419-54C4-4389-B38C-59DDB5E7D5E9@yahoo.com> Message-ID: If your in a hospital: I've always had it verified with the microbiology department if it hasn't been done already(if not then still the following) Then it needs to be reported to your local department of health and the CDC since it is a reportable disease. I'm sure others can expand on this but this is what I've had to do. Kim Donadio Sent from my iPhone On Jan 20, 2012, at 6:47 AM, Michele Email wrote: > Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated. > Thank you > Michele Carr > > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Fri Jan 20 10:45:54 2012 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Fri Jan 20 10:46:02 2012 Subject: [Histonet] Vantage Printers Message-ID: <53FC421CC200C5429929EDE6C3676F3001BBE95C@msgebe34> Hi Histoland, For those of you that are using the Vantage from Ventana, what cassette printers have you found work the best? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor - Pathology Department MOHS Lab Supervisor - Dermatology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org From Bauer.Karen <@t> mayo.edu Fri Jan 20 10:49:44 2012 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Fri Jan 20 10:49:48 2012 Subject: [Histonet] Tissue Tek Xpress Message-ID: <53FC421CC200C5429929EDE6C3676F3001BBE95E@msgebe34> Hi, My future plan for our lab is to purchase a Tissue Tek Xpress for continuous processing. I'd like to hear from Xpress50 users and Xpress120 users. What are your processing schedules? What do you process throughout the day? Would you recommend two 50's instead of one 120? Any thoughts or comments are appreciated. Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor - Pathology Department MOHS Lab Supervisor - Dermatology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 20 11:00:12 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jan 20 11:00:22 2012 Subject: [Histonet] RE: Tissue Tek Xpress In-Reply-To: <53FC421CC200C5429929EDE6C3676F3001BBE95E@msgebe34> References: <53FC421CC200C5429929EDE6C3676F3001BBE95E@msgebe34> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE6CC@MCINFRWEM003.ucsfmedicalcenter.org> I'm interested in this as well, but how about fixation times for various biopsies if continuous processing is used? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Friday, January 20, 2012 8:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Tek Xpress Importance: Low Hi, My future plan for our lab is to purchase a Tissue Tek Xpress for continuous processing. I'd like to hear from Xpress50 users and Xpress120 users. What are your processing schedules? What do you process throughout the day? Would you recommend two 50's instead of one 120? Any thoughts or comments are appreciated. Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor - Pathology Department MOHS Lab Supervisor - Dermatology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jan 20 12:18:29 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jan 20 12:18:34 2012 Subject: [Histonet] Galectin 3 and Tripsin Message-ID: Hi Histonet, Wondering if anyone out there would be willing to share a company and protocol for Galectin 3 and Trypsin IHC's on paraffin embedded tissue. Thanks in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From LBUSTAMANTE <@t> cvm.tamu.edu Fri Jan 20 13:14:34 2012 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Fri Jan 20 13:14:36 2012 Subject: [Histonet] Microtome Reicher-jung 2030 Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC939C39C5E@CVMMB02.cvm.tamu.edu> I am looking to buy 2 of this microtome in very good condition please. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Lab Supervisor College Of Veterinary Medicine Texas A&M University Phone (979) 845-3177 Fax (979) 458-3499 lbustamante@cvm.tamu.edu From lanigac <@t> ccf.org Fri Jan 20 13:17:23 2012 From: lanigac <@t> ccf.org (Lanigan, Christopher) Date: Fri Jan 20 13:17:41 2012 Subject: [Histonet] K/L bone marrow Message-ID: <9DDD0F026DC71B41B79D8E439D7951A30BAB46ED@cchsclexmb69.cc.ad.cchs.net> -----Original Message----- Date: Wed, 18 Jan 2012 08:29:48 -0500 From: Clare Thornton Subject: [Histonet] K/L bone marrow To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have a Kappa/Lambda bone marrow ISH protocol for use on the Ventana Benchmark XT? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Reply----- Hi Clare, Sorry for the delay. Yes, I have a successful Kappa / Lambda ISH protocol for Bone Marrow on the Ventana Benchmark XT. First, the software that you will need to have installed is called "XT ISH Open Probes ChromogenicV3". This protocol will use the iView BLUE+ DETECTION and with a RED STAIN II counterstain. Before I get to the protocol, I'll fill you in on the Kappa and Lambda probes. They do NOT arrive in a dispenser. They each arrive in 4 pre-diluted vials, so you will need to fill a "user-fillable dispenser". All four pre-diluted vials are mixed together into one user-fillable dispenser. Apparently, Ventana is required to package each separately. Additionally, you will need to run a control. This control will confirm the presence of non-degradated RNA. The control I used was U6, and the protocol is identical to the Kappa or Lambda except for the selected ISH probe. By the way, the selected ISH probe will likely be "ISH PROBE 1" or "ISH PROBE 2" because the user-fillable dispenser will not have the proper commercial bar code. One last thing, I was concerned initially about Bone Marrow adhesion to the charged slides, so I ran a comparison of 2 leading brands. The clear winner was Superfrost Plus distributed by Cardinal Health. Finally, the following is a successful protocol summary. 1 Baking [Selected] 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking ) 3 Deparaffinization [Selected] 4 Standard [Selected] 5 Warmup Slide to [69 Deg C], and Incubate for 4 Minutes ( Deparaffinization ) 6 Enzyme [Selected] 7 Apply Coverslip, One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate for [8 Minutes] 8 Probe [Selected] 9 Probe Auto Dispense [Selected] 10 1 Drop of Probe Dispensed [Selected] 11 Apply One Drop of [ISH Probe 1] ( ISH Probe ), Apply Coverslip, and Incubate for 4 Minutes 12 Denature [Selected] 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( Denaturation ) 14 Hybridization [Selected] 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 16 Incubate for [1 Hour] ( Hybridization ) 17 Stringency Washes [Selected] 18 Stringency Wash #1 [Selected] 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 20 Stringency Wash #2 [Selected] 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) 22 Stringency Wash #3 [Selected] 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) 24 Detection Kit [Selected] 25 Blue Detection [Selected] 26 Incubate for [32 Minutes] ( Substrate ) 27 Counterstain [Selected] 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 29 Post Run LCS Application [Selected] Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News & World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From one_angel_secret <@t> yahoo.com Fri Jan 20 16:25:52 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jan 20 16:26:11 2012 Subject: [Histonet] Tuberculosis positive tissue In-Reply-To: References: <99606419-54C4-4389-B38C-59DDB5E7D5E9@yahoo.com> Message-ID: <8876DEB4-0C35-4867-93BA-46A4FDE6CD71@yahoo.com> Oops. 10% bleach to clean. They do have some stuff called tuberculicide < spelled wrong I'm sure. Oh and get a tine test to see if you had exposure. Sorry for my half reply earlier. Was on break at work with ten things in head. Hope you havnt had an exposure Best wishes Kim Donadio Sent from my iPhone On Jan 20, 2012, at 10:06 AM, Kim Donadio wrote: > If your in a hospital: I've always had it verified with the microbiology department if it hasn't been done already(if not then still the following) Then it needs to be reported to your local department of health and the CDC since it is a reportable disease. I'm sure others can expand on this but this is what I've had to do. > Kim Donadio > > Sent from my iPhone > > On Jan 20, 2012, at 6:47 AM, Michele Email wrote: > >> Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated. >> Thank you >> Michele Carr >> >> >> Sent from my iPad >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerry.santiago <@t> bellsouth.net Fri Jan 20 18:21:50 2012 From: jerry.santiago <@t> bellsouth.net (Jerry Santiago) Date: Fri Jan 20 18:21:53 2012 Subject: [Histonet] Correct web address for FSH Message-ID: <1327105310.35635.YahooMailRC@web181711.mail.ne1.yahoo.com> Sorry guys, I posted the wrong link o the Florida Society for Histotechnology website. The web address is www.fshgroup.org. From one_angel_secret <@t> yahoo.com Fri Jan 20 20:43:21 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jan 20 20:46:24 2012 Subject: [Histonet] RE: slide file storage to dry slides In-Reply-To: References: Message-ID: <35472AE1-2384-4B12-B444-3145805CDB84@yahoo.com> Yeah. I want this median too. Thanks for heads up Kim Sent from my iPhone On Jan 19, 2012, at 10:25 AM, DKBoyd@chs.net wrote: > Joyce, > Interesting! What methodology are using to remove the coverslip and with > what difficulty? I may be interested in changing to this medium. Are you > using this same medium with Non-gyn Cytology and have you had any bleeding > problems? Also we do not use Xylene. We use a substitute. > Thanks! > Debbie > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > "Weems, Joyce" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/19/2012 09:58 AM > > To > Sebree Linda A , "Histonet@lists.utsouthwestern.edu" > > cc > > Subject > [Histonet] RE: slide file storage to dry slides > > > > > > > We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050 > 102 - that doesn't need extra drying. File the next day with no sticking.. > j > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Thursday, January 19, 2012 09:26 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] slide file storage to dry slides > > Good morning all, > > We've recently switched from film coverslipping back to glass and > therefore need to thoroughly dry our slides before permanent filing. I > recall, in my first histology job....30 + years ago, that we used metal > stacking slide files that you could put an insert into the drawers that > looked like a non-stretchy spring. The wires of this "spring" held the > slides apart to dry, then they could be filed without the "spring" when > they were completely dry. > > Anyone know if that product still exists? Or does anyone have a better > solution for drying slides while still keeping them in order? > > Thanks for the assist, > > Linda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri Jan 20 20:50:43 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jan 20 20:50:53 2012 Subject: [Histonet] slide file storage to dry slides In-Reply-To: References: Message-ID: <64B3FBD5-2057-4108-B96E-BEF9EBCC6B55@yahoo.com> I've seen this a couple ways. Metal trays put in slide drying oven on low temp overnight. Low or you will ruin some labels . I've also seen these nice wooden boxes that hold the metal slide trays. You put them in it , it's like a rack . Put them in order. I think they hold about 1000 slides. You just leave these there they will be your most recent. By the time it's full. Put half up and then continue rotating after that. I'd check with fisher maybe Nite nite Kim Sent from my iPhone On Jan 19, 2012, at 9:25 AM, "Sebree Linda A" wrote: > Good morning all, > > We've recently switched from film coverslipping back to glass and > therefore need to thoroughly dry our slides before permanent filing. I > recall, in my first histology job....30 + years ago, that we used metal > stacking slide files that you could put an insert into the drawers that > looked like a non-stretchy spring. The wires of this "spring" held the > slides apart to dry, then they could be filed without the "spring" when > they were completely dry. > > Anyone know if that product still exists? Or does anyone have a better > solution for drying slides while still keeping them in order? > > Thanks for the assist, > > Linda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jan 21 10:39:03 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jan 21 10:39:10 2012 Subject: [Histonet] sample woes Message-ID: <43722E05B4DA45048652C318FB96543D@prueggihctechlt> Please advise. I was sent a bunch of sample by an investigator who is not too sharp. The samples were prepared thus: Pancreases were removed, washed placed in a modified Zamboni fixative (2% formaldehyde, 15%Picric acid in 0.1M PBS, pH 7.5). Sample portions were cut from the head, body and tail of the pancreas in 3 mm by 3 mm sections. The pancreatic tissue samples from the various regions of the pancreas were fixed overnight; samples were equilibrated in 50% sucrose in 0.01M PBS for 12 hours at 4?C, and mounted in Tissue Tek OCT Compound (Miles Inc.); 0.015 mm (15 ?m) thick sections were obtained by cryostat sectioning. SAMPLES WERE FROZEN IN 50% Tissue Tek 50% sucrose , then shipped on wet ice, not dry ice. These frozen tissue samples were in small embedding molds with a tiny dab of the OCT/sucrose gel on top of them, it looked like none was under them. They shipped these supposedly previous frozen samples to me on wet ice, not dry ice (dumb move), the molds were open on top in a box, the sample thawed and the gel and sample was leaking out of their molds, it was a mess. The investigator who sent the samples to me said this "the samples arrived within 18hrs as signed for, so they should have still been frozen", how this person got to be the CEO of a Biotech company I cannot imagine. They wanted me to just refreeze them and cryosection them to test some abs they sent with them, I said that I would not do that, I did agree to take 2 samples as a pilot study and fix them in formalin overnight and then process and embed them in paraffin, which I have done. If I can get them to section (they are still soft and squishy ) I was planning on doing an H&E stain and look at the samples to see if they look preserved at all. My question to you all is this: if the tissue looks viable by H&E what should I try to test immuno reactivity viability? I was thinking of running an antibody like vimentin or cytokeratin or something, but these are ms pancreas so maybe I should use insulin ab or something else. Thank you for your valuable advise, Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From pruegg <@t> ihctech.net Sat Jan 21 11:04:58 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jan 21 11:05:03 2012 Subject: [Histonet] A nit to pick - background IHC staining In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009EE2828@EX4.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009EE2828@EX4.lj.gnf.org> Message-ID: <94A5152BB90D45D2A421C0173304B708@prueggihctechlt> The MOM kits may minimize this but they do not eliminate the non specific binding completely in my experience, macrophages and plasma cells are particularly difficult to eliminate staining in. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, January 06, 2012 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A nit to pick - background IHC staining Happy Friday to you all! I just wanted to comment on the idea that when detecting mouse antibodies on mouse tissues gives you background staining. I consider background staining to be non-specific binding of some reagent to the tissue that is then detected with the chromogen or fluorophore. Anti-mouse antibodies specifically bind to the mouse Igs in the tissue as well as to the mouse Ig labeled antigen from the antibody. It's a nuisance and not specific to your target, but I don't consider it background. As previously mentioned, mouse on mouse kits work well to minimize this. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jan 21 11:11:29 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jan 21 11:11:35 2012 Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? In-Reply-To: References: Message-ID: <9B4BBD01D2E94FD3BE86E17A799414CC@prueggihctechlt> I use the tape transfer system. What tissues are you trying to cut? If you are cutting soft tissues you should be using the 0.5 or 1x coated slides, the 4x and above are for bone. Have you tried putting your slides on a block of dryice after exposing them to uv, let them sit for a while to get really cold, then carefully pull the tape off while on the dryice I pull diagonally from corner to corner very slowly and smoothly. Cut the sections as thin as possible and do not press hard on the tape rolled onto the block but I do press hard and roll the heck of the tape on the coated slides, then expose to the uv 3 or 4 times before removing the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns Sent: Wednesday, January 04, 2012 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Hello, Histonetters, To continue my (sad) string posts about CryoJane problems, we have now switched to the 4X slides, and we still observe the tape pulling pieces of the section off the slide. Sometimes the pieces are very small, sometimes they are large, and at times a whole region of the section comes off with the tape. We have now tried many different things to correct this: 1) pulling the tape off the section in many different ways, 2) pulling tape off at many different angles & speeds, 3) several different temperatures, 4) with many different mental attitudes, 5) with sections of different thickness, 6) light rolling of tape and section versus ferocious rolling, etc., etc. So far, no dice; we are puzzled. Does anyone have more ideas about what to adjust. We think that this really should work, but at the same time, we can't seem to locate many users of the CryoJane system. So, CryoJane enthusiasts, this is the time to talk about why you like it, or how you do it. thanks again --------------- Doug Burns, MBRF, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jan 21 11:13:52 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jan 21 11:13:57 2012 Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? References: Message-ID: <0A13102785964D6F8F4AF4DCAE56A872@prueggihctechlt> I forgot to mention that I always use a D profile tungsten carbide permanent knife to make these sections. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Saturday, January 21, 2012 10:11 AM To: 'Douglas M Burns'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? I use the tape transfer system. What tissues are you trying to cut? If you are cutting soft tissues you should be using the 0.5 or 1x coated slides, the 4x and above are for bone. Have you tried putting your slides on a block of dryice after exposing them to uv, let them sit for a while to get really cold, then carefully pull the tape off while on the dryice I pull diagonally from corner to corner very slowly and smoothly. Cut the sections as thin as possible and do not press hard on the tape rolled onto the block but I do press hard and roll the heck of the tape on the coated slides, then expose to the uv 3 or 4 times before removing the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns Sent: Wednesday, January 04, 2012 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Hello, Histonetters, To continue my (sad) string posts about CryoJane problems, we have now switched to the 4X slides, and we still observe the tape pulling pieces of the section off the slide. Sometimes the pieces are very small, sometimes they are large, and at times a whole region of the section comes off with the tape. We have now tried many different things to correct this: 1) pulling the tape off the section in many different ways, 2) pulling tape off at many different angles & speeds, 3) several different temperatures, 4) with many different mental attitudes, 5) with sections of different thickness, 6) light rolling of tape and section versus ferocious rolling, etc., etc. So far, no dice; we are puzzled. Does anyone have more ideas about what to adjust. We think that this really should work, but at the same time, we can't seem to locate many users of the CryoJane system. So, CryoJane enthusiasts, this is the time to talk about why you like it, or how you do it. thanks again --------------- Doug Burns, MBRF, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Sat Jan 21 15:32:44 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Jan 21 15:32:51 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 28 In-Reply-To: <4f1afd54.c39dec0a.3916.ffff8e11SMTPIN_ADDED@mx.google.com> References: <4f1afd54.c39dec0a.3916.ffff8e11SMTPIN_ADDED@mx.google.com> Message-ID: Re: CAP/ANP Policies Hello All, I am desperate for helps! I just started my new job 6 months ago, and our lab has inspected by CAP. During their inspection, I received a big surprise because they found no written policies for Histology in our Q-Pulse. :( :( Obversely, we have a lot of deficiencies, mostly LACK of written policies, but no violations. :) I am swam with IHC/ISH QC & optimization on bench, and absolutely NO time for written policies (English is not my first language & strong skill). I would greatly appreciated if anyone would share their histology written policies with me (used as Template only & confidential). Following are our deficiencies that I need to submit to them within a month (probably need extension). General Quality Control ANP #s'; ANP.21050, 21100, 21150, 21350, 21366, 21382, 21390, 21395 Immunohistochemistry ANP #s'; ANP.22250, 22550, 22570, 22660, 22750, 22760, 22800, 22900, 22990, 22993 Equipment Maintenance ANP #s'; ANP.23048, 23050, 23075 Tissue Processor ANP #s'; ANP.23100, 23150 Paraffin Dispenser ANP #s'; ANP.23200, 23250, 23300 Floatation Baths ANP #s'; ANP.23350 Microtomes ANP #s'; ANP.23450 Automated Tissue Processor ANP #s'; ANP.24050 Mostly greatly any helps! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Jan 21, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Galectin 3 and Tripsin (McMahon, Loralee A) > ? 2. Microtome Reicher-jung 2030 (Bustamante, Lin) > ? 3. K/L bone marrow (Lanigan, Christopher) > ? 4. Re: Tuberculosis positive tissue (Kim Donadio) > ? 5. Correct web address for FSH (Jerry Santiago) > ? 6. Re: RE: slide file storage to dry slides (Kim Donadio) > ? 7. Re: slide file storage to dry slides (Kim Donadio) > ? 8. sample woes (Patsy Ruegg) > ? 9. RE: A nit to pick - background IHC staining (Patsy Ruegg) > ?10. RE: Are there any CryoJane users out there who understand the > ? ? ?quirks of the tape? (Patsy Ruegg) > ?11. RE: Are there any CryoJane users out there who understand the > ? ? ?quirks of the tape? (Patsy Ruegg) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 20 Jan 2012 13:18:29 -0500 > From: "McMahon, Loralee A" > Subject: [Histonet] Galectin 3 and Tripsin > To: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonet, > > Wondering if anyone out there would be willing to share a company and protocol for Galectin 3 and Trypsin IHC's on paraffin embedded tissue. > Thanks in advance. > > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > > ------------------------------ > > Message: 2 > Date: Fri, 20 Jan 2012 19:14:34 +0000 > From: "Bustamante, Lin" > Subject: [Histonet] Microtome Reicher-jung 2030 > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<94B6DC15AAF2F046BF847D4C1CA9AAC939C39C5E@CVMMB02.cvm.tamu.edu> > Content-Type: text/plain; charset="us-ascii" > > I am looking to buy 2 of this microtome in very good condition please. > Thank you. > Lin. > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Lab Supervisor > College Of Veterinary Medicine > Texas A&M University > Phone (979) 845-3177 > Fax (979) 458-3499 > lbustamante@cvm.tamu.edu > > > > ------------------------------ > > Message: 3 > Date: Fri, 20 Jan 2012 14:17:23 -0500 > From: "Lanigan, Christopher" > Subject: [Histonet] K/L bone marrow > To: histonet@lists.utsouthwestern.edu > Cc: CThornton@dahlchase.com > Message-ID: > ? ? ? ?<9DDD0F026DC71B41B79D8E439D7951A30BAB46ED@cchsclexmb69.cc.ad.cchs.net> > Content-Type: text/plain; charset=us-ascii > > -----Original Message----- > > Date: Wed, 18 Jan 2012 08:29:48 -0500 > > From: Clare Thornton > > Subject: [Histonet] K/L bone marrow > > To: "'histonet@lists.utsouthwestern.edu'" > > ? ? > > Message-ID: > > ? ? > > Content-Type: text/plain; charset="us-ascii" > > > > Does anyone have a Kappa/Lambda bone marrow ISH protocol for use on the > Ventana Benchmark XT? > > > > thanks! > > Clare > > > > Clare J. Thornton, HTL(ASCP), QIHC > > Assistant Histology Supervisor > > Dahl-Chase Diagnostic Services > > 417 State Street, Suite 540 > > Bangor, ME 04401 > > cthornton@dahlchase.com > > > > -----Reply----- > > > > Hi Clare, > > > > Sorry for the delay. > > > > Yes, I have a successful Kappa / Lambda ISH protocol for Bone Marrow on > the Ventana Benchmark XT. > > > > First, the software that you will need to have installed is called "XT > ISH Open Probes ChromogenicV3". ?This protocol will use the iView BLUE+ > DETECTION and with a RED STAIN II counterstain. > > > > Before I get to the protocol, I'll fill you in on the Kappa and Lambda > probes. ?They do NOT arrive in a dispenser. ?They each arrive in 4 > pre-diluted vials, so you will need to fill a "user-fillable dispenser". > All four pre-diluted vials are mixed together into one user-fillable > dispenser. ?Apparently, Ventana is required to package each separately. > > > > Additionally, you will need to run a control. ?This control will confirm > the presence of non-degradated RNA. ?The control I used was U6, and the > protocol is identical to the Kappa or Lambda except for the selected ISH > probe. > > > > By the way, the selected ISH probe will likely be "ISH PROBE 1" or "ISH > PROBE 2" because the user-fillable dispenser will not have the proper > commercial bar code. > > > > One last thing, I was concerned initially about Bone Marrow adhesion to > the charged slides, so I ran a comparison of 2 leading brands. ?The > clear winner was Superfrost Plus distributed by Cardinal Health. > > > > Finally, the following is a successful protocol summary. > > > > 1 Baking [Selected] > > 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking ) > > 3 Deparaffinization [Selected] > > 4 Standard [Selected] > > 5 Warmup Slide to [69 Deg C], and Incubate for 4 Minutes ( > Deparaffinization ) > > 6 Enzyme [Selected] > > 7 Apply Coverslip, One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate > for [8 Minutes] > > 8 Probe [Selected] > > 9 Probe Auto Dispense [Selected] > > 10 1 Drop of Probe Dispensed [Selected] > > 11 Apply One Drop of [ISH Probe 1] ( ISH Probe ), Apply Coverslip, and > Incubate for 4 Minutes > > 12 Denature [Selected] > > 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( > Denaturation ) > > 14 Hybridization [Selected] > > 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( > Hybridization ) > > 16 Incubate for [1 Hour] ( Hybridization ) > > 17 Stringency Washes [Selected] > > 18 Stringency Wash #1 [Selected] > > 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency > Wash #1 ) > > 20 Stringency Wash #2 [Selected] > > 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) > > 22 Stringency Wash #3 [Selected] > > 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) > > 24 Detection Kit [Selected] > > 25 Blue Detection [Selected] > > 26 Incubate for [32 Minutes] ( Substrate ) > > 27 Counterstain [Selected] > > 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, > and Incubate for [4 Minutes] > > 29 Post Run LCS Application [Selected] > > > > Christopher Lanigan > > Research Technologist > > Molecular Pathology > > Cleveland Clinic Foundation > > 9500 Euclid Avenue L3-127 > > Cleveland, OH 44195 > > > > > > > > > =================================== > > ?Please consider the environment before printing this e-mail > > Cleveland Clinic is ranked one of the top hospitals > in America by U.S.News & World Report (2010). > Visit us online at http://www.clevelandclinic.org for > a complete listing of our services, staff and > locations. > > > Confidentiality Note: ?This message is intended for use > only by the individual or entity to which it is addressed > and may contain information that is privileged, > confidential, and exempt from disclosure under applicable > law. ?If the reader of this message is not the intended > recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. ?If > you have received this communication in error, ?please > contact the sender immediately and destroy the material in > its entirety, whether electronic or hard copy. ?Thank you. > > > ------------------------------ > > Message: 4 > Date: Fri, 20 Jan 2012 17:25:52 -0500 > From: Kim Donadio > Subject: Re: [Histonet] Tuberculosis positive tissue > To: Kim Donadio > Cc: "Histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <8876DEB4-0C35-4867-93BA-46A4FDE6CD71@yahoo.com> > Content-Type: text/plain; ? ? ? charset=us-ascii > > Oops. 10% bleach to clean. They do have some stuff called tuberculicide < spelled wrong I'm sure. ?Oh and get a tine test to see if you had exposure. Sorry for my half reply earlier. Was on break at work with ten things in head. ?Hope you havnt had an exposure > Best wishes > Kim Donadio > > Sent from my iPhone > > On Jan 20, 2012, at 10:06 AM, Kim Donadio wrote: > >> If your in a hospital: I've always had it verified with the microbiology department if it hasn't been done already(if not then still the following) Then it needs to be reported to your local department of health and the CDC since it is a reportable disease. I'm sure others can expand on this but this is what I've had to do. >> Kim Donadio >> >> Sent from my iPhone >> >> On Jan 20, 2012, at 6:47 AM, Michele Email wrote: >> >>> Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. ? What Decontamination procedures do you perform? ?Also what about documentation? ?Any help would be appreciated. >>> Thank you >>> Michele Carr >>> >>> >>> Sent from my iPad >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Fri, 20 Jan 2012 16:21:50 -0800 (PST) > From: Jerry Santiago > Subject: [Histonet] Correct web address for FSH > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ?<1327105310.35635.YahooMailRC@web181711.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Sorry guys, > > I posted the wrong link o the Florida Society for Histotechnology website. The > web address is www.fshgroup.org. > > > ------------------------------ > > Message: 6 > Date: Fri, 20 Jan 2012 21:43:21 -0500 > From: Kim Donadio > Subject: Re: [Histonet] RE: slide file storage to dry slides > To: "DKBoyd@chs.net" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?"Weems, Joyce" > Message-ID: <35472AE1-2384-4B12-B444-3145805CDB84@yahoo.com> > Content-Type: text/plain; ? ? ? charset=us-ascii > > Yeah. I want this median too. Thanks for heads up > Kim > > Sent from my iPhone > > On Jan 19, 2012, at 10:25 AM, DKBoyd@chs.net wrote: > >> Joyce, >> Interesting! ?What methodology are using to remove the coverslip and with >> what difficulty? ?I may be interested in changing to this medium. ?Are you >> using this same medium with Non-gyn Cytology and have you had any bleeding >> problems? ? Also we do not use Xylene. ?We use a substitute. >> Thanks! >> Debbie >> >> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical >> Center I >> 200 Medical Park Boulevard l Petersburg, Va. ?23805 l T: 804-765-5050 l F: >> 804-765-5582 l dkboyd@chs.net >> >> >> >> >> >> >> >> "Weems, Joyce" >> Sent by: histonet-bounces@lists.utsouthwestern.edu >> 01/19/2012 09:58 AM >> >> To >> Sebree Linda A , "Histonet@lists.utsouthwestern.edu" >> >> cc >> >> Subject >> [Histonet] RE: slide file storage to dry slides >> >> >> >> >> >> >> We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050 >> 102 - that doesn't need extra drying. File the next day with no sticking.. >> j >> >> >> Joyce Weems >> Pathology Manager >> Saint Joseph's Hospital >> 5665 Peachtree Dunwoody Rd NE >> Atlanta, GA 30342 >> 678-843-7376 - Phone >> 678-843-7831 - Fax >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree >> Linda A >> Sent: Thursday, January 19, 2012 09:26 >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] slide file storage to dry slides >> >> Good morning all, >> >> We've recently switched from film coverslipping back to glass and >> therefore need to thoroughly dry our slides before permanent filing. ?I >> recall, in my first histology job....30 + years ago, that we used metal >> stacking slide files that you could put an insert into the drawers that >> looked like a non-stretchy spring. ?The wires of this "spring" held the >> slides apart to dry, then they could be filed without the "spring" when >> they were completely dry. >> >> Anyone know if that product still exists? ?Or does anyone have a better >> solution for drying slides while still keeping them in order? >> >> Thanks for the assist, >> >> Linda >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This e-mail, including any attachments is the >> property of Catholic Health East and is intended >> for the sole use of the intended recipient(s). >> It may contain information that is privileged and >> confidential. ?Any unauthorized review, use, >> disclosure, or distribution is prohibited. If you are >> not the intended recipient, please delete this message, and >> reply to the sender regarding the error in a separate email. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> -------------------------------------------------------------------------- >> Disclaimer: This electronic message may contain information that is >> Proprietary, Confidential, or legally privileged or protected. It >> is intended only for the use of the individual(s) and entity named >> in the message. If you are not an intended recipient of this >> message, please notify the sender immediately and delete the >> material from your computer. Do not deliver, distribute or copy >> this message and do not disclose its contents or take any action in >> reliance on the information it contains. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 20 Jan 2012 21:50:43 -0500 > From: Kim Donadio > Subject: Re: [Histonet] slide file storage to dry slides > To: Sebree Linda A > Cc: "" > ? ? ? ? > Message-ID: <64B3FBD5-2057-4108-B96E-BEF9EBCC6B55@yahoo.com> > Content-Type: text/plain; ? ? ? charset=us-ascii > > I've seen this a couple ways. Metal trays put in slide drying oven on low temp overnight. Low or you will ruin some labels . I've also seen these nice wooden boxes that hold the metal slide trays. You put them in it , it's like a rack . Put them in order. I think they hold about 1000 slides. You just leave these there they will be your most recent. By the time it's full. Put half up and then continue rotating after that. I'd check with fisher maybe > Nite nite > Kim > > Sent from my iPhone > > On Jan 19, 2012, at 9:25 AM, "Sebree Linda A" wrote: > >> Good morning all, >> >> We've recently switched from film coverslipping back to glass and >> therefore need to thoroughly dry our slides before permanent filing. ?I >> recall, in my first histology job....30 + years ago, that we used metal >> stacking slide files that you could put an insert into the drawers that >> looked like a non-stretchy spring. ?The wires of this "spring" held the >> slides apart to dry, then they could be filed without the "spring" when >> they were completely dry. >> >> Anyone know if that product still exists? ?Or does anyone have a better >> solution for drying slides while still keeping them in order? >> >> Thanks for the assist, >> >> Linda >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Sat, 21 Jan 2012 09:39:03 -0700 > From: "Patsy Ruegg" > Subject: [Histonet] sample woes > To: "'Histonet'" > Message-ID: <43722E05B4DA45048652C318FB96543D@prueggihctechlt> > Content-Type: text/plain; ? ? ? charset="iso-8859-7" > > Please advise. > > > > I was sent a bunch of sample by an investigator who is not too sharp. The > samples were prepared thus: > > > > Pancreases were removed, washed placed in a modified Zamboni fixative (2% > formaldehyde, 15%Picric acid in 0.1M PBS, pH 7.5). Sample portions were cut > from the head, body and tail of the pancreas in 3 mm by 3 mm sections. > > The pancreatic tissue samples from the various regions of the pancreas were > fixed overnight; samples were equilibrated in 50% sucrose in 0.01M PBS for > 12 hours at 4?C, and mounted in Tissue Tek OCT Compound (Miles Inc.); 0.015 > mm (15 ?m) thick sections were obtained by cryostat sectioning. > > SAMPLES WERE FROZEN IN 50% Tissue Tek 50% sucrose , then shipped on wet ice, > not dry ice. > > These frozen tissue samples were in small embedding molds with a tiny dab of > the OCT/sucrose gel on top of them, it looked like none was under them. They > shipped these supposedly previous frozen samples to me on wet ice, not dry > ice (dumb move), the molds were open on top in a box, the sample thawed and > the gel and sample was leaking out of their molds, it was a mess. The > investigator who sent the samples to me said this "the samples arrived > within 18hrs as signed for, so they should have still been frozen", how this > person got to be the CEO of a Biotech company I cannot imagine. > > > > They wanted me to just refreeze them and cryosection them to test some abs > they sent with them, I said that I would not do that, I did agree to take 2 > samples as a pilot study and fix them in formalin overnight and then process > and embed them in paraffin, which I have done. If I can get them to section > (they are still soft and squishy ) I was planning on doing an H&E stain and > look at the samples to see if they look preserved at all. My question to you > all is this: if the tissue looks viable by H&E what should I try to test > immuno reactivity viability? I was thinking of running an antibody like > vimentin or cytokeratin or something, but these are ms pancreas so maybe I > should use insulin ab or something else. > > > > Thank you for your valuable advise, > > > > Regards, > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. Ste.215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > www.ihctech.net > > www.ihcrg.org > > > > > > ------------------------------ > > Message: 9 > Date: Sat, 21 Jan 2012 10:04:58 -0700 > From: "Patsy Ruegg" > Subject: RE: [Histonet] A nit to pick - background IHC staining > To: "'Theresa \(Teri\) Johnson'" , > ? ? ? ? > Message-ID: <94A5152BB90D45D2A421C0173304B708@prueggihctechlt> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > The MOM kits may minimize this but they do not eliminate the non specific > binding completely in my experience, macrophages and plasma cells are > particularly difficult to eliminate staining in. > > Regards, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa > (Teri) Johnson > Sent: Friday, January 06, 2012 9:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] A nit to pick - background IHC staining > > Happy Friday to you all! > > I just wanted to comment on the idea that when detecting mouse antibodies on > mouse tissues gives you background staining. I consider background staining > to be non-specific binding of some reagent to the tissue that is then > detected with the chromogen or fluorophore. Anti-mouse antibodies > specifically bind to the mouse Igs in the tissue as well as to the mouse Ig > labeled antigen from the antibody. > > It's a nuisance and not specific to your target, but I don't consider it > background. As previously mentioned, mouse on mouse kits work well to > minimize this. > > Teri Johnson, HT(ASCP)QIHC > GNF Histology Lab Manager > Genomics Institute of the Novartis Research Foundation > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Sat, 21 Jan 2012 10:11:29 -0700 > From: "Patsy Ruegg" > Subject: RE: [Histonet] Are there any CryoJane users out there who > ? ? ? ?understand ? ? ?the quirks of the tape? > To: "'Douglas M Burns'" , > ? ? ? ? > Message-ID: <9B4BBD01D2E94FD3BE86E17A799414CC@prueggihctechlt> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > I use the tape transfer system. ?What tissues are you trying to cut? ?If you > are cutting soft tissues you should be using the 0.5 or 1x coated slides, > the 4x and above are for bone. > > Have you tried putting your slides on a block of dryice after exposing them > to uv, let them sit for a while to get really cold, then carefully pull the > tape off while on the dryice I pull diagonally from corner to corner very > slowly and smoothly. ?Cut the sections as thin as possible and do not press > hard on the tape rolled onto the block but I do press hard and roll the heck > of the tape on the coated slides, then expose to the uv 3 or 4 times before > removing the tape. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M > Burns > Sent: Wednesday, January 04, 2012 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Are there any CryoJane users out there who understand > the quirks of the tape? > > Hello, Histonetters, > > ? ? To continue my (sad) string posts about CryoJane problems, we have now > switched to the 4X slides, and we still observe the tape pulling pieces of > the section off the slide. Sometimes the pieces are very small, sometimes > they are large, and at times a whole region of the section comes off with > the tape. > > ? ? ?We have now tried many different things to correct this: ?1) pulling > the tape off the section in many different ways, 2) pulling tape off at > many different angles & speeds, 3) several different temperatures, 4) with > many different mental attitudes, 5) with sections of different thickness, > 6) light rolling of tape and section versus ferocious rolling, etc., etc. > So far, no dice; we are puzzled. > > ? ? ?Does anyone have more ideas about what to adjust. We think that this > really should work, but at the same time, we can't seem to locate many > users of the CryoJane system. So, CryoJane enthusiasts, this is the time to > talk about why you like it, or how you do it. > > ? ? ? ? ? ? ? ? ? ? ? ?thanks again ? --------------- ? Doug Burns, MBRF, > Kansas City > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 11 > Date: Sat, 21 Jan 2012 10:13:52 -0700 > From: "Patsy Ruegg" > Subject: RE: [Histonet] Are there any CryoJane users out there who > ? ? ? ?understand ? ? ?the quirks of the tape? > To: "'Patsy Ruegg'" , ? ? ? "'Douglas M Burns'" > ? ? ? ?, ? > Message-ID: <0A13102785964D6F8F4AF4DCAE56A872@prueggihctechlt> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > I forgot to mention that I always use a D profile tungsten carbide permanent > knife to make these sections. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: Patsy Ruegg [mailto:pruegg@ihctech.net] > Sent: Saturday, January 21, 2012 10:11 AM > To: 'Douglas M Burns'; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Are there any CryoJane users out there who > understand the quirks of the tape? > > I use the tape transfer system. ?What tissues are you trying to cut? ?If you > are cutting soft tissues you should be using the 0.5 or 1x coated slides, > the 4x and above are for bone. > > Have you tried putting your slides on a block of dryice after exposing them > to uv, let them sit for a while to get really cold, then carefully pull the > tape off while on the dryice I pull diagonally from corner to corner very > slowly and smoothly. ?Cut the sections as thin as possible and do not press > hard on the tape rolled onto the block but I do press hard and roll the heck > of the tape on the coated slides, then expose to the uv 3 or 4 times before > removing the tape. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M > Burns > Sent: Wednesday, January 04, 2012 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Are there any CryoJane users out there who understand > the quirks of the tape? > > Hello, Histonetters, > > ? ? To continue my (sad) string posts about CryoJane problems, we have now > switched to the 4X slides, and we still observe the tape pulling pieces of > the section off the slide. Sometimes the pieces are very small, sometimes > they are large, and at times a whole region of the section comes off with > the tape. > > ? ? ?We have now tried many different things to correct this: ?1) pulling > the tape off the section in many different ways, 2) pulling tape off at > many different angles & speeds, 3) several different temperatures, 4) with > many different mental attitudes, 5) with sections of different thickness, > 6) light rolling of tape and section versus ferocious rolling, etc., etc. > So far, no dice; we are puzzled. > > ? ? ?Does anyone have more ideas about what to adjust. We think that this > really should work, but at the same time, we can't seem to locate many > users of the CryoJane system. So, CryoJane enthusiasts, this is the time to > talk about why you like it, or how you do it. > > ? ? ? ? ? ? ? ? ? ? ? ?thanks again ? --------------- ? Doug Burns, MBRF, > Kansas City > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 28 > **************************************** From arunjyothisp <@t> gmail.com Sun Jan 22 06:06:20 2012 From: arunjyothisp <@t> gmail.com (Arun Jyothi S.P) Date: Sun Jan 22 06:06:28 2012 Subject: [Histonet] Reg: Acidified and Nonacidified Harris Hematoxylin - Adv & Disadv Message-ID: Dear Histonet experts, Can you please tell me what are the advantages and disadvantages of both acidified and non acidified Harris hematoxylin. We are a new lab and in the process of ordering and standardising things. It will be a great help if someone let us know us which one we should order and what are the benefits reg the hematoxylin. It will be more helpful if i can find out which one preferred the most by the pathologists. With regards ARUN JYOTHI S.P. Histotechnologist United Laboratories Co. Kuwait From SDattili <@t> stormontvail.org Mon Jan 23 08:01:41 2012 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Mon Jan 23 08:01:49 2012 Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 29 In-Reply-To: <25b168c70002c636@stormontvail.org> Message-ID: Hi all, Madeleine asked the group to share policies with her. Madeleine, I would advise you to sit down with your medical director and tell him or her exactly what is going on in the lab. It is frankly almost unbelievable to me that your medical director did not know about the state of the procedures. He or she needs to get you some help NOW. I also advise you to finish a couple of simple procedures to document your serious intent and request an extension from CAP. A schedule of how you will finish your procedures will help CAP grant your extension. 1. Maintenance procedures for equipment are specific for the equipment you are using. Check the website of your equipment manufacturer. Many manufacturers provide their procedures online in an editable format. Look for "CLSI" procedure or something like that. Download the procedure and customize it for your own lab. The account rep for your big equipment might be another source of information. In addition to a procedure, you need a checklist or other form to document that the maintenance was performed. 2. The explanations in the CAP checklist are excellent resources for writing procedures. Often the "Principle/Purpose" section of your procedure can be lifted directly from the CAP checklist. Download your lab's customized checklist from www.cap.org. 3. Ask your IHC instrument account rep if the company can send a technical specialist to help you with optimization and validation of your stains so that you can have extra time to work on procedures. I will send some procedures to you directly to get you started. I hope that you find them helpful. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From claycal44 <@t> yahoo.com Mon Jan 23 08:20:17 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Mon Jan 23 08:20:26 2012 Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? In-Reply-To: <0A13102785964D6F8F4AF4DCAE56A872@prueggihctechlt> References: <0A13102785964D6F8F4AF4DCAE56A872@prueggihctechlt> Message-ID: <1327328417.89864.YahooMailNeo@web164520.mail.gq1.yahoo.com> I also use the CryoJane and have problems similar to yours.? I get much better bone sections with it, but? they never seem to be perfect like others say they get.? I also have tried many different ways to do it, but there always seems to be a part that does not completely transfer from the tape to the slide. Nancy.lowen@va.gov From: Patsy Ruegg To: 'Patsy Ruegg' ; 'Douglas M Burns' ; histonet@lists.utsouthwestern.edu Sent: Saturday, January 21, 2012 9:13 AM Subject: RE: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? I forgot to mention that I always use a D profile tungsten carbide permanent knife to make these sections. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Saturday, January 21, 2012 10:11 AM To: 'Douglas M Burns'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? I use the tape transfer system.? What tissues are you trying to cut?? If you are cutting soft tissues you should be using the 0.5 or 1x coated slides, the 4x and above are for bone. Have you tried putting your slides on a block of dryice after exposing them to uv, let them sit for a while to get really cold, then carefully pull the tape off while on the dryice I pull diagonally from corner to corner very slowly and smoothly.? Cut the sections as thin as possible and do not press hard on the tape rolled onto the block but I do press hard and roll the heck of the tape on the coated slides, then expose to the uv 3 or 4 times before removing the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns Sent: Wednesday, January 04, 2012 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Hello, Histonetters, ? ? To continue my (sad) string posts about CryoJane problems, we have now switched to the 4X slides, and we still observe the tape pulling pieces of the section off the slide. Sometimes the pieces are very small, sometimes they are large, and at times a whole region of the section comes off with the tape. ? ? ? We have now tried many different things to correct this:? 1) pulling the tape off the section in many different ways, 2) pulling tape off at many different angles & speeds, 3) several different temperatures, 4) with many different mental attitudes, 5) with sections of different thickness, 6) light rolling of tape and section versus ferocious rolling, etc., etc. So far, no dice; we are puzzled. ? ? ? Does anyone have more ideas about what to adjust. We think that this really should work, but at the same time, we can't seem to locate many users of the CryoJane system. So, CryoJane enthusiasts, this is the time to talk about why you like it, or how you do it. ? ? ? ? ? ? ? ? ? ? ? ? thanks again? ---------------? Doug Burns, MBRF, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dea.leslie <@t> gmail.com Mon Jan 23 08:42:57 2012 From: dea.leslie <@t> gmail.com (Deanna Leslie) Date: Mon Jan 23 08:43:05 2012 Subject: [Histonet] PRN work in Cincinnati, OH Message-ID: I am in search of an experienced HT to cover scheduled absences, etc. in my small research lab. Very relaxed setting for a small group of scientists. Please contact me for further information. Deanna Leslie HT ASCP Lead Tech Shriners Hospital for Children Shared Histology facility 513-872-6388 From Carol.Fields <@t> Northside.com Mon Jan 23 08:47:38 2012 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Mon Jan 23 08:48:57 2012 Subject: [Histonet] Breast Fixation with fixatives other than Formalin Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> Hi Netters, Could you please let me know who all is using breast fixatives other than 10% formalin and what do you do about CAP requirements? I would also like to get in touch with Dr. Richard Cartun because I think he has done studies on this for Her2. Our docs will not let us use anything other than formalin because of CAP regs and the result is a lot of raw breast tissue. This is even holding the tissue for a couple days. Any help with this would be appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From AHutton <@t> dh.org Mon Jan 23 08:55:05 2012 From: AHutton <@t> dh.org (Hutton, Allison) Date: Mon Jan 23 08:57:34 2012 Subject: [Histonet] Breast Fixation with fixatives other than Formalin In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> Message-ID: <38A56C4F4630D348A50B3720409270870E0FE500@dhmail.dhorg.org> I would really like to know this also, We only use formalin and we have alot of problems with our breast tissue. Thanks Allison -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Carol Fields Sent: Monday, January 23, 2012 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast Fixation with fixatives other than Formalin Hi Netters, Could you please let me know who all is using breast fixatives other than 10% formalin and what do you do about CAP requirements? I would also like to get in touch with Dr. Richard Cartun because I think he has done studies on this for Her2. Our docs will not let us use anything other than formalin because of CAP regs and the result is a lot of raw breast tissue. This is even holding the tissue for a couple days. Any help with this would be appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Jan 23 09:01:24 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Jan 23 09:03:54 2012 Subject: [Histonet] RE: Breast Fixation with fixatives other than Formalin In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> Message-ID: Are you prepping the breast tissue upon receipt? We bread loaf our larger peices and place into formalin in large containers to fix overnight. Rarely do we have a reprocess a block. Mostly we find that the reprocessing comes from the peices of tissue that are being put into the cassettes are too large. Then the do no process well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields [Carol.Fields@Northside.com] Sent: Monday, January 23, 2012 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast Fixation with fixatives other than Formalin Hi Netters, Could you please let me know who all is using breast fixatives other than 10% formalin and what do you do about CAP requirements? I would also like to get in touch with Dr. Richard Cartun because I think he has done studies on this for Her2. Our docs will not let us use anything other than formalin because of CAP regs and the result is a lot of raw breast tissue. This is even holding the tissue for a couple days. Any help with this would be appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Jan 23 09:29:50 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jan 23 09:30:04 2012 Subject: [Histonet] RE: Breast Fixation with fixatives other than Formalin In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE6DB@MCINFRWEM003.ucsfmedicalcenter.org> Carole, The CAP "regs" are recommendations, not regulations. They give you an out to use any other fixative as long as you do a complete validation of the alternative fixative, ie prove the Her2/ER/PR results are identical with the alternative fixative compared to formalin-fixed tissue. That will entail a lot of work on your part but it can be done. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Monday, January 23, 2012 6:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast Fixation with fixatives other than Formalin Hi Netters, Could you please let me know who all is using breast fixatives other than 10% formalin and what do you do about CAP requirements? I would also like to get in touch with Dr. Richard Cartun because I think he has done studies on this for Her2. Our docs will not let us use anything other than formalin because of CAP regs and the result is a lot of raw breast tissue. This is even holding the tissue for a couple days. Any help with this would be appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Mon Jan 23 09:50:28 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Jan 23 09:50:38 2012 Subject: [Histonet] Breast Fixation with fixatives other than Formalin In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> Message-ID: 10% Formalin for the fixative and Xylene in the tissue processing are the FDA approved method for Her2. That said, you have to properly manage the tissue and fixative or adding days to the process will not improve the result. I would suggest that you approach your problem form a different aspect. 10% formalin is a fine fixative for breast tissue, but it will not penetrate you tissue samples that are greater then 5 cm thick. ASCO/CAP suggest that for larger samples, >5 cm, the tissue should be "loafed" into slices not greater than 5 cm for fixation. This provides only 2.5 cm of distance the fixative needs to travel. I am a strong proponent that the samples selected for processing and placed into the cassette should be <3 mm thick. If you dissect the specimen and the tissue is not "fixed", then once the sample is in the cassette, make sure you have adequate fixation, stay w/in the ASCO/CAP guidelines, but at least 6 hrs fixation when you get the "raw" <3mm sample in the cassette. I would also look at the processing schedule you use for tissue sample processing. With thick specimens, >3mm, the schedule will need to be extended, 10-12 hrs total processing time, and you may continue to get varying results. If you select thin samples and apply adequate fixation time once you have a sample in the cassette, you will get consistent results. With proper sample size and fixation you can consistently process to paraffin in less than 2 hrs, using the Xpress rapid tissue processor. Standardize and create precision in your sampling and fixation and I know you will have superior and consistent results. William DeSalvo, B.S., HTL(ASCP) > Date: Mon, 23 Jan 2012 09:47:38 -0500 > From: Carol.Fields@Northside.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breast Fixation with fixatives other than Formalin > > Hi Netters, > Could you please let me know who all is using breast fixatives other > than 10% formalin and what do you do about CAP requirements? I would > also like to get in touch with Dr. Richard Cartun because I think he has > done studies on this for Her2. Our docs will not let us use anything > other than formalin because of CAP regs and the result is a lot of raw > breast tissue. This is even holding the tissue for a couple days. > Any help with this would be appreciated. > Thank you, > Carole > > Carole Fields, HT (ASCP) > Histology Supervisor > Northside Hospital > Atlanta, GA 30342 > carol.fields@northside.com > > > > > CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Jan 23 10:07:58 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Jan 23 10:10:01 2012 Subject: [Histonet] Breast Fixation with fixatives other than Formalin In-Reply-To: References: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3235D32820@Mail2Node2.ad.uams.edu> We bread loaf each breast and then allow nothing to be processed with less than 6 hours in the cassette plus processor time for formalin. It is a rule if the breast is not in cassettes before 3PM it cannot go on the processor. We follow the guidelines and in most cases the breast fix overnight, loafed. Then the residents will cassette it for processing leaving us well within the 48 hour maximum for Her2Neu. If they are late on Friday we process Saturday and take the tissue off on Sunday so we bent no rule for the formalin fixation. The pathologists were unhappy with the idea and then they stopped getting raw meat with really bad artifact so they are now happy. We did not look at alternatives due to the validation process. If we could get our residents to properly gross thinner breast (and all) all tissue we could possibly shorten some times. Since they don't gross thin enough they have learned to like Histology better. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, January 23, 2012 9:50 AM To: carol.fields@northside.com; histonet Subject: RE: [Histonet] Breast Fixation with fixatives other than Formalin 10% Formalin for the fixative and Xylene in the tissue processing are the FDA approved method for Her2. That said, you have to properly manage the tissue and fixative or adding days to the process will not improve the result. I would suggest that you approach your problem form a different aspect. 10% formalin is a fine fixative for breast tissue, but it will not penetrate you tissue samples that are greater then 5 cm thick. ASCO/CAP suggest that for larger samples, >5 cm, the tissue should be "loafed" into slices not greater than 5 cm for fixation. This provides only 2.5 cm of distance the fixative needs to travel. I am a strong proponent that the samples selected for processing and placed into the cassette should be <3 mm thick. If you dissect the specimen and the tissue is not "fixed", then once the sample is in the cassette, make sure you have adequate fixation, stay w/in the ASCO/CAP guidelines, but at least 6 hrs fixation when you get the "raw" <3mm sample in the cassette. I would also look at the processing schedule you use for tissue sample processing. With thick specimens, >3mm, the schedule will need to be extended, 10-12 hrs total processing time, and you may continue to get varying results. If you select thin samples and apply adequate fixation time once you have a sample in the cassette, you will get consistent results. With proper sample size and fixation you can consistently process to paraffin in less than 2 hrs, using the Xpress rapid tissue processor. Standardize and create precision in your sampling and fixation and I know you will have superior and consistent results. William DeSalvo, B.S., HTL(ASCP) > Date: Mon, 23 Jan 2012 09:47:38 -0500 > From: Carol.Fields@Northside.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breast Fixation with fixatives other than Formalin > > Hi Netters, > Could you please let me know who all is using breast fixatives other > than 10% formalin and what do you do about CAP requirements? I would > also like to get in touch with Dr. Richard Cartun because I think he > has done studies on this for Her2. Our docs will not let us use > anything other than formalin because of CAP regs and the result is a > lot of raw breast tissue. This is even holding the tissue for a couple days. > Any help with this would be appreciated. > Thank you, > Carole > > Carole Fields, HT (ASCP) > Histology Supervisor > Northside Hospital > Atlanta, GA 30342 > carol.fields@northside.com > > > > > CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From TJohnson <@t> gnf.org Mon Jan 23 10:16:20 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Mon Jan 23 10:16:30 2012 Subject: [Histonet] Re: sample woes Message-ID: <9F3CFEE76E51B64991C7485270890B4009EEDC0D@EX5.lj.gnf.org> Patsy, In my opinion, the samples should have been fine to re-freeze provided they had no freezing artifact from the first time they were frozen. They were fixed and cryoprotected so they might just turn out fine. It certainly might have been worth a try to take a sample, equilibrate it in 100% OCT and try snap freezing first, cutting a section and H&E staining to look at the morphology. I'm thinking that it might have been just fine. Removing the OCT and sucrose for paraffin sectioning should have been fairly easy. I am surprised they are still soft and squishy after processing. As for what antibody to try, what species are these pancreas samples from? Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From mamawooo <@t> hotmail.com Mon Jan 23 10:45:03 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Mon Jan 23 10:45:11 2012 Subject: [Histonet] Vantage Printers In-Reply-To: <53FC421CC200C5429929EDE6C3676F3001BBE95C@msgebe34> References: <53FC421CC200C5429929EDE6C3676F3001BBE95C@msgebe34> Message-ID: ThermoShandon printmate.Jan,Omaha > Date: Fri, 20 Jan 2012 10:45:54 -0600 > From: Bauer.Karen@mayo.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Vantage Printers > > Hi Histoland, > > For those of you that are using the Vantage from Ventana, what cassette > printers have you found work the best? > > Thanks much!! > > Karen > > Karen L. Bauer HTL/HT (ASCP) > Histology Supervisor - Pathology Department > MOHS Lab Supervisor - Dermatology Department > Mayo Clinic Health System in Eau Claire > Phone: 715-838-3205 > E-mail: bauer.karen@mayo.edu > ___________________________________________ > Mayo Clinic Health System > 1221 Whipple St. > Eau Claire, WI 54703 > www.mayoclinichealthsystem.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Mon Jan 23 11:23:26 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jan 23 11:23:31 2012 Subject: [Histonet] RE: Breast Fixation with fixatives other than Formalin In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE6DB@MCINFRWEM003.ucsfmedicalcenter.org> References: <731941C266951A47BEF11E5EFAAED9C90B666E9A@nsmvexch01.northside.local> <8D7C2D242DBD45498006B21122072BF89F5EE6DB@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E1EA@CHEXCMS10.one.ads.che.org> However, formalin is the only FDA approved fixative and patients can miss clinical trials if it is not used - at least that is my understanding of the issue. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, January 23, 2012 10:30 To: 'Carol Fields'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Breast Fixation with fixatives other than Formalin Carole, The CAP "regs" are recommendations, not regulations. They give you an out to use any other fixative as long as you do a complete validation of the alternative fixative, ie prove the Her2/ER/PR results are identical with the alternative fixative compared to formalin-fixed tissue. That will entail a lot of work on your part but it can be done. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Monday, January 23, 2012 6:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast Fixation with fixatives other than Formalin Hi Netters, Could you please let me know who all is using breast fixatives other than 10% formalin and what do you do about CAP requirements? I would also like to get in touch with Dr. Richard Cartun because I think he has done studies on this for Her2. Our docs will not let us use anything other than formalin because of CAP regs and the result is a lot of raw breast tissue. This is even holding the tissue for a couple days. Any help with this would be appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sdysart <@t> mirnarx.com Mon Jan 23 11:26:23 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Jan 23 11:27:08 2012 Subject: [Histonet] Xylene and Preggers Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001CF353C@BL2PRD0710MB363.namprd07.prod.outlook.com> Hey all, so 2 things... A. Does anyone have anything saying that .75% (yes less than 1) is an acceptable exposure limit for a pregnant person and B. Does HR have the right to tell people that you are pregnant after you ask them questions (ie. Your manager, and all the way up??) Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From cforster <@t> umn.edu Mon Jan 23 11:45:19 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jan 23 11:45:21 2012 Subject: [Histonet] Pearls Prussian blue with green counterstain Message-ID: <4F1D9CAF.7000408@umn.edu> I am wondering if any of you have ever used a Fast Green counter stain in place of the Nuclear Fast Red? If so, what timing did you use? Thanks, Colleen Forster U of MN From karabou76 <@t> hotmail.com Mon Jan 23 12:59:09 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Mon Jan 23 12:59:14 2012 Subject: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently. In-Reply-To: References: Message-ID: Tissue is fixed in 10% NBF before being vacuum embedded in paraffin. Our tissue is cut at 10microns, placed on charged slides, then placed on a slide warmer over night. The slides are then place in xylenes 3 times for 2 minutes, then stained as follows..Step 4: 100% Alcohol ? 2 X 2 minutes each,Step 5: 95% Alcohol ? 2 X 2 minutes each,Step 6: DI H2O ? 2 X 2 minutes each,Step 7: Harris Hematoxylin ? 1 X 1.5 minutes,Step 8: Wash gently in DI H2O until?Grape Juice? color is gone, Step 9: Acid Alcohol ? 3 Dips, Step 10: Wash gently in DI H2O ? 1 X 2 minutes, Step 11: Bluing ? 10 Dips, Step 12: Rinse in running DI H2O ? 1 X 2minutes, Step 13: 95% Alcohol ? 1 X 2 minutes ,Step 14: Working Eosin ? 1 X 2 minutes, Step 15: 95% Alcohol ? 2 X 2 minutes each, Step 16: 100% Alcohol ? 3 X 2 minutes each,Step 17: Xylene Dips ? 3 X 5 minutes each, Step 18: Coverslip. Our core lab has recently had a change in pressure for the DI port and water comes out very hard, making gentle washing impossible. The reagents are new. We have tried increasing the staining time in the hematoxylin to 2 minutes and reducing the acid alcohol dips to 2. Our hematoxylin is not consistently staining the nuclei in the ligament tissue. Some are good, some are bad. Can someone make any suggestions? From kdboydhisto <@t> yahoo.com Mon Jan 23 13:08:55 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Mon Jan 23 13:09:03 2012 Subject: [Histonet] LIS Message-ID: <1327345735.82705.YahooMailNeo@web125803.mail.ne1.yahoo.com> In today's Histology world, what is?the best LIS and the worst LIS and why???? From joelleweaver <@t> hotmail.com Mon Jan 23 13:14:37 2012 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Mon Jan 23 13:14:41 2012 Subject: [Histonet] LIS Message-ID: I personally don't like meditech, when I used it, it did not respond to mouse commands, was not browser navigated and could not migrate data from one module to another within the same system. It seemed to be incompatible with all instrumentation interfaces, without additional customized middleware and could be customized, for the end using facility, but ONLY at great expense. I understand it was better for use in the clinical lab. Just an opinion Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Kelly Boyd Date: Mon, 23 Jan 2012 19:08:55 To: Subject: [Histonet] LIS In today's Histology world, what is?the best LIS and the worst LIS and why???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Mon Jan 23 13:44:13 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Mon Jan 23 13:44:18 2012 Subject: [Histonet] Re: xylene and preggers Message-ID: <9F3CFEE76E51B64991C7485270890B4009EEF4D9@EX5.lj.gnf.org> Sarah, To answer your first question, what is .75%? The permissible exposure limit for xylene is 100 ppm (435 mg/cubic meter of air) for an 8-hour time-weighted average, and 150 ppm (655 mg/m3) for a 15 minute short term exposure limit. How does .75% fit in to that? (Sorry to answer a question with a question) The second question is a little trickier. A quick google search will show that there are a lot of answers to this one. Some issues require a judgment call with how to proceed. Their primary responsibility is to protect the company. They may take a "need to know" approach to some issues. So in this case, they may give a heads up to a manager if they think there is an issue they may need to deal with. In my opinion, health issues should be protected information as is other sensitive employee info such as pay rates and performance evals. Remember that managers are usually privy to this information as well. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From mpence <@t> grhs.net Mon Jan 23 13:51:00 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jan 23 13:51:05 2012 Subject: [Histonet] LIS In-Reply-To: <1327345735.82705.YahooMailNeo@web125803.mail.ne1.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974D96@is-e2k3.grhs.net> This is like asking which car is the best to buy! I think everyone will have their pros and cons to each system. I suggest you find 3-5 and have them demo what they have. You are going to get what you pay for. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Monday, January 23, 2012 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS In today's Histology world, what is?the best LIS and the worst LIS and why???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Jan 23 13:58:07 2012 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Mon Jan 23 13:58:11 2012 Subject: [Histonet] LIS Message-ID: Meant no harm to those with other opinions, Kelly asked for "opinions". Doing a demo is a great idea, won't know until then if it works for you. I would just keep in mind the data transfer, HL7, EMR and CPOE capabilities as well as user "issues" and preferences such as those in my opinions. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Mike Pence Date: Mon, 23 Jan 2012 19:51:00 To: ; Subject: RE: [Histonet] LIS This is like asking which car is the best to buy! I think everyone will have their pros and cons to each system. I suggest you find 3-5 and have them demo what they have. You are going to get what you pay for. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Monday, January 23, 2012 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS In today's Histology world, what is?the best LIS and the worst LIS and why???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Mon Jan 23 15:08:01 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Jan 23 15:08:13 2012 Subject: [Histonet] Grossing question Message-ID: <24A4826E8EF0964D86BC5317306F58A55FDBC15DA4@mmc-mail.ad.mhsil.com> Our pathologists are wanting to know what other places do with regards to one part of our gross descriptions. Before CoPath we always had to dictate at the first that the specimen container and the req each had the patient name..... (John Doe). If the req. was slightly different than the containers such as one might have a proper name and one might have the legal name we had to state that and also dictate how we determined what the legal name was. (such as comparing demographic material, calling the patient, etc.) When Copath started up we changed to only using a req. and specimen template that simply said, The req. and specimen container each have the same patient name. We did not dictate small discrepancies such as noted before. CoPath allowed us to document the information but in order to clean up our gross descriptions we did not enter the problem in the gross description unless it was a major problem such as the site designated on the req. does not match the site listed on the container. We are also using CoPath's automated barcoded tracking which of course has many identifiers. We also require that all containers have two forms of identification, such as name, DOB, site of biopsy, alternate id number, ss. Security number, etc when submitted to the pathology department. It is also protocol that the grossers look at all containers and the req to compare identifiers. Difference between now and the past is that we do not include this in the typed gross description. What the pathologists want to know is what are others dictating? We will be implementing Dragon dictation soon and have heard of other institutions using a general template at the beginning of the gross description. Could anyone share what they are doing with their gross descriptions? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Carol.Fields <@t> Northside.com Mon Jan 23 15:24:25 2012 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Mon Jan 23 15:25:40 2012 Subject: [Histonet] Fixative for Breast Tissue Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666EA0@nsmvexch01.northside.local> THX to all for your helpful answers today. It was appreciated. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From tony.henwood <@t> health.nsw.gov.au Mon Jan 23 16:38:28 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jan 23 16:38:41 2012 Subject: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently. In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16282@xmdb02.nch.kids> Have a look at the dewaxing part of the protocol. Is the xylene removing all the wax? If wax is incompletely removed from the sections then nuclei will be poorly stained whereas, interestingly the eosin counterstain will seem to be unaffected (though with a diligent look you will see poorer eosin staining as well - look at the contrast between collagen, smooth muscle and nerves). After xylene and alcohol, check the water rinsed slide. The tissue should not be hydrophobic. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Tuesday, 24 January 2012 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently. Tissue is fixed in 10% NBF before being vacuum embedded in paraffin. Our tissue is cut at 10microns, placed on charged slides, then placed on a slide warmer over night. The slides are then place in xylenes 3 times for 2 minutes, then stained as follows..Step 4: 100% Alcohol - 2 X 2 minutes each,Step 5: 95% Alcohol - 2 X 2 minutes each,Step 6: DI H2O - 2 X 2 minutes each,Step 7: Harris Hematoxylin - 1 X 1.5 minutes,Step 8: Wash gently in DI H2O until"Grape Juice" color is gone, Step 9: Acid Alcohol - 3 Dips, Step 10: Wash gently in DI H2O - 1 X 2 minutes, Step 11: Bluing - 10 Dips, Step 12: Rinse in running DI H2O - 1 X 2minutes, Step 13: 95% Alcohol - 1 X 2 minutes ,Step 14: Working Eosin - 1 X 2 minutes, Step 15: 95% Alcohol - 2 X 2 minutes each, Step 16: 100% Alcohol - 3 X 2 minutes each,Step 17: Xylene Dips - 3 X 5 minutes each, Step 18: Coverslip. Our core lab has recently had a change in pressure for the DI port and water comes out very hard, making gentle washing impossible. The reagents are new. We have tried increasing the staining time in the hematoxylin to 2 minutes and reducing the acid alcohol dips to 2. Our hematoxylin is not consistently staining the nuclei in the ligament tissue. Some are good, some are bad. Can someone make any suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Mon Jan 23 17:05:17 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jan 23 17:05:24 2012 Subject: [Histonet] Pearls Prussian blue with green counterstain In-Reply-To: <4F1D9CAF.7000408@umn.edu> References: <4F1D9CAF.7000408@umn.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A162CC@xmdb02.nch.kids> I would have thought that Perls with a fast green counterstain would lack contrast (green and blue?0 Rather than nuclear fast red (which I find very pretty) you could use 1% neutral red in 2% acetic acid , or 1% Bismark Brown in 1% acetic acid, or even 15sec in your H&E eosin solution. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Colleen Forster Sent: Tuesday, 24 January 2012 4:45 AM To: Histonet Subject: [Histonet] Pearls Prussian blue with green counterstain I am wondering if any of you have ever used a Fast Green counter stain in place of the Nuclear Fast Red? If so, what timing did you use? Thanks, Colleen Forster U of MN ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From one_angel_secret <@t> yahoo.com Mon Jan 23 17:22:00 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Jan 23 17:22:04 2012 Subject: [Histonet] LIS In-Reply-To: References: Message-ID: <1327360920.42115.YahooMailNeo@web112307.mail.gq1.yahoo.com> yep and it never hurts to call around to a few hospitals your size and ask them what they are using and how do they like it. Usually you'll get good info from either the manager or any other staff. These things are often a nightmare to get up and running, then all the training so make sure who ever you choose, you also feel like they will give you good after care service. ? For Path stuff Ive used Cerner mostly and I liked it. ? ? ________________________________ From: joelle weaver To: Mike Pence ; "kdboydhisto@yahoo.com " ; "histonet@lists.utsouthwestern.edu " Sent: Monday, January 23, 2012 2:58 PM Subject: Re: [Histonet] LIS Meant no harm to those with other opinions, Kelly asked for "opinions". Doing a demo is a great idea, won't know until then if it works for you. I would just keep in mind the data transfer, HL7, EMR and CPOE capabilities as well as user "issues" and preferences such as those in my opinions. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Mike Pence Date: Mon, 23 Jan 2012 19:51:00 To: ; Subject: RE: [Histonet] LIS This is like asking which car is the best to buy! I think everyone will have their pros and cons to each system. I suggest you find 3-5 and have them demo what they have. You are going to get what you pay for. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Monday, January 23, 2012 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS In today's Histology world, what is?the best LIS and the worst LIS and why???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robyn.Rosenberg <@t> amnhealthcare.com Mon Jan 23 17:50:12 2012 From: Robyn.Rosenberg <@t> amnhealthcare.com (Robyn Rosenberg) Date: Mon Jan 23 17:50:22 2012 Subject: [Histonet] Please remove my email address from the list. Message-ID: <2030E2FECB4DAB48B4C31D919380F3FF1245C8FE@amnmail.ahs.int> Dear Histonet, Please remove my email address from the list. Thank you Robyn Rosenberg Recruiter, Recruitment Process Outsourcing AMN Healthcare Direct Phone: (858) 314-7460 Direct Fax: (866) 652-6931 12400 High Bluff Drive, San Diego CA 92130 robyn.rosenberg@amnhealthcare.com www.amnhealthcare.com NYSE: AHS Connect with me at: www.linkedin.com/in/robynr1 www.linkedin.com/Healthcare Careers Resource From Mdsher <@t> aol.com Mon Jan 23 18:20:57 2012 From: Mdsher <@t> aol.com (Mdsher@aol.com) Date: Mon Jan 23 18:21:02 2012 Subject: [Histonet] Remove My Name From List Message-ID: <47b5.57ad55af.3c4f5369@aol.com> Dear Histonet, Please remove my name from this list. Thank-you, Miriam From nunut86 <@t> hotmail.com Tue Jan 24 03:45:29 2012 From: nunut86 <@t> hotmail.com (Natalia Fernandez) Date: Tue Jan 24 03:45:36 2012 Subject: [Histonet] Efficacity of fluorochrome in Immunofluorescence Message-ID: Hi everybody! Maybe you can help me. I'm trying to do immunofluorescences to see the colocalisation between two proteins (PSD + Oligomeres) using Oregon Green (goat anti mouse) and Texas Red (goat anti rabbit). But my probleme, is that the analysis of the slides reveals too much colocalisation. I mean, all seems yellow, as if both fluorochromes show the same thing. It's the first time I do fluorescence, so I don't know where could come the problem. I tried to do a simple staining, 1 slide with my first antibody and its fluorochrome, and the second slide with the other first antibody with its fluorochome and then check the result with the filter red and green. But it's strange because I see both color as if I had put both fluorochromes (second antibodies). Has somebody already had this problem? What can I do to resolve it? Thank you very much for your help. Natalia From kryan <@t> nfderm.com Tue Jan 24 06:30:15 2012 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Tue Jan 24 06:30:21 2012 Subject: [Histonet] Dermatology software Message-ID: HI, We are beginning to look at a computer program for our private Dermatology organization and I was wondering if you would share your ideas on a good derm program to explore? We have five offices in our organization but only one lab and right now we do all accessioning by hand. I am looking for something with good tracking and the ability to will give me workload data. Any suggestions would be appreciated! Kaye Ryan, Lab Manager North Florida Dermatology Associates, PA From Erin.Martin <@t> ucsf.edu Tue Jan 24 09:54:35 2012 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Jan 24 09:54:53 2012 Subject: [Histonet] MCP antibody? Message-ID: <24B7B291CC88D04AB663958E77A1F59D023A5F@ex09.net.ucsf.edu> Good morning everyone, Does anyone use MCP antibody (Merkel Cell Polyoma Virus) from a vendor other than Santa Cruz Biotech? If so, would you please let me know your source? Thank you! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From Melissa.Kuhnla <@t> chsli.org Tue Jan 24 11:30:15 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Tue Jan 24 11:30:43 2012 Subject: [Histonet] (no subject) Message-ID: Hello All, I have completed my 2011 statistical analysis of our ER/PR results, comparing them to 'published benchmarks' as per CAP/ASCO guidelines. Has anyone also performed this for their Her2 testing? What benchmarks did you use? Can you site your source? We are currently just running Her2 FISH. Thank you :-) :-) Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From HornHV <@t> archildrens.org Tue Jan 24 11:52:34 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jan 24 11:52:44 2012 Subject: [Histonet] job opening Message-ID: <25A4DE08332B19499904459F00AAACB719ACB9CBBE@EVS1.archildrens.org> Arkansas Children's Hospital has a position for a full time histology tech. This is a 8:30 am until 5:00 pm shift Monday through Friday. We are a small friendly lab who works well together. ACH offers excellent benefits. Apply online at www.archildrens.org Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kmerriam2003 <@t> yahoo.com Tue Jan 24 12:46:26 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Jan 24 12:46:34 2012 Subject: [Histonet] Efficacity of fluorochrome in Immunofluorescence References: Message-ID: <1327430786.65017.YahooMailNeo@web130101.mail.mud.yahoo.com> You are likely getting some cross-reactivity with your secondary antibodies?(one of them may be crossing to the other primary).? We always run a battery of control slides when validating a double-IF stain.? Run these controls and you will likely figure out where the problem is: ? Slide Set Antibodies 1 Primary #1/Secondary#1 2 Primary#2/Secondary #2 3 Isotype#1/Secondary #1 4 Isotype #2/Secondary #2 5 Primary #1/Primary #2/Secondary #1/Secondary#2 6 Isotype #1/Isotype #2/Secondary#1/Secondary #2 7 Primary #1/Isotype #2/Secondary #1/Secondary#2 8 Isotype #1/Primary#2/Secondary #1/Secondary #2 9 NoPrimary (diluentonly)/Secondary #1 10 No Primary(diluentonly)/Secondary #2 11 No Primary (diluentonly)/Secondary #1/Secondary #2 12 Diluent only (or nothing) 13 DAPI only ? Best of luck! Kim, Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Natalia Fernandez To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 24, 2012 4:45 AM Subject: [Histonet] Efficacity of fluorochrome in Immunofluorescence Hi everybody! Maybe you can help me. I'm trying to do immunofluorescences to see the colocalisation between two proteins (PSD + Oligomeres) using Oregon Green (goat anti mouse) and Texas Red (goat anti rabbit). But my probleme, is that the analysis of the slides reveals too much colocalisation. I mean, all seems yellow, as if both fluorochromes show the same thing. It's the first time I do fluorescence, so I don't know where could come the problem. I tried to do a simple staining, 1 slide with my first antibody and its fluorochrome, and the second slide with the other first antibody with its fluorochome and then check the result with the filter red and green. But it's strange because I see both color as if I had put both fluorochromes (second antibodies). Has somebody already had this problem? What can I do to resolve it? Thank you very much for your help. Natalia ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karabou76 <@t> hotmail.com Tue Jan 24 13:02:42 2012 From: karabou76 <@t> hotmail.com (Kara Lee) Date: Tue Jan 24 13:02:47 2012 Subject: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently. In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A16282@xmdb02.nch.kids> References: , , <6D6BD1DE8A5571489398B392A38A715760A16282@xmdb02.nch.kids> Message-ID: Thanks for the advice! We have been worried that wax was the issue. I do leave them in 3 sets of xylene for 5 minutes each, you would think that would get rid of the wax. Is it ok to leave them in the xylenes for longer than 5 minutes each? Thanks again for the advice to everyone who responded so far :) > From: tony.henwood@health.nsw.gov.au > To: karabou76@hotmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently. > Date: Mon, 23 Jan 2012 22:38:28 +0000 > > Have a look at the dewaxing part of the protocol. > Is the xylene removing all the wax? > If wax is incompletely removed from the sections then nuclei will be poorly stained whereas, interestingly the eosin counterstain will seem to be unaffected (though with a diligent look you will see poorer eosin staining as well - look at the contrast between collagen, smooth muscle and nerves). > > After xylene and alcohol, check the water rinsed slide. The tissue should not be hydrophobic. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kara Lee > Sent: Tuesday, 24 January 2012 5:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question regarding staining of ligament tissue using H&E. The nuclei in our ligament tissue is not staining consistently. > > > > > > > > > > > > > > Tissue is fixed in 10% NBF before being vacuum embedded in paraffin. Our tissue is cut at 10microns, placed on charged slides, then placed on a slide warmer over night. The slides are then place in xylenes 3 times for 2 minutes, then stained as follows..Step 4: 100% Alcohol - 2 X 2 minutes each,Step 5: 95% Alcohol - 2 X 2 minutes each,Step 6: DI H2O - 2 X 2 minutes each,Step 7: Harris Hematoxylin - 1 X 1.5 minutes,Step 8: Wash gently in DI H2O until"Grape Juice" color is gone, Step 9: Acid Alcohol - 3 Dips, Step 10: Wash gently in DI H2O - 1 X 2 minutes, Step 11: Bluing - 10 Dips, Step 12: Rinse in running DI H2O - 1 X 2minutes, Step 13: 95% Alcohol - 1 X 2 minutes ,Step 14: Working Eosin - 1 X 2 minutes, Step 15: 95% Alcohol - 2 X 2 minutes each, Step 16: 100% Alcohol - 3 X 2 minutes each,Step 17: Xylene Dips - 3 X 5 minutes each, Step 18: Coverslip. > Our core lab has recently had a change in pressure for the DI port and water comes out very hard, making gentle washing impossible. The reagents are new. We have tried increasing the staining time in the hematoxylin to 2 minutes and reducing the acid alcohol dips to 2. > Our hematoxylin is not consistently staining the nuclei in the ligament tissue. Some are good, some are bad. > > Can someone make any suggestions? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* From MSHERWOOD <@t> PARTNERS.ORG Tue Jan 24 13:51:11 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Jan 24 13:51:16 2012 Subject: [Histonet] OCT embedded frozen tissue for paraffin embedding Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5D89@PHSXMB30.partners.org> One of our investigators wants to take his frozen tissue (embedded in OCT) and now fix in formalin for paraffin embedding. I assume he needs to wash out the OCT (?phosphate buffer that formalin is prepared in) and then fix in 10% formalin. I would appreciate feedback from the listers. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sdysart <@t> mirnarx.com Tue Jan 24 13:57:19 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Jan 24 13:57:29 2012 Subject: [Histonet] OCT embedded frozen tissue for paraffin embedding In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5D89@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5D89@PHSXMB30.partners.org> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001CF38FA@BL2PRD0710MB363.namprd07.prod.outlook.com> Yes, just wash out the OCT and fix as normal...Just remember that these tissues might not look the same as normal fixed tissues because they were frozen first. Sometimes you can get cracking or freezing artifact. Good Luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, January 24, 2012 1:51 PM To: histonet Subject: Re: [Histonet] OCT embedded frozen tissue for paraffin embedding One of our investigators wants to take his frozen tissue (embedded in OCT) and now fix in formalin for paraffin embedding. I assume he needs to wash out the OCT (?phosphate buffer that formalin is prepared in) and then fix in 10% formalin. I would appreciate feedback from the listers. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amario3 <@t> uic.edu Tue Jan 24 14:29:06 2012 From: amario3 <@t> uic.edu (Andrea Marion) Date: Tue Jan 24 14:29:10 2012 Subject: [Histonet] Re: Efficacity of fluorochrome in Immunofluorescence Message-ID: <3f2e2a196311e8ba5eb67e177d97b6c3.squirrel@webmail.uic.edu> Hi Natalia, What type of tissue are you working with, and how was it processed? Are these paraffin sections or cryosections? What fixative did you use? These details can help narrow down a potential problem. If you post your protocol that will help. You might be seeing autofluorescence from the tissue itself, especially if you are working with paraffin sections or formaldehyde-fixed samples. See what the tissue looks like with no secondary antibodies - if you see the same fluorescence, you have autofluorescence coming from the tissue itself. You will need to either process the tissue differently, try to block the autofluorescence, or amplify the signal of your antibody staining above the level of the autofluorescence (using avidin/biotin or TSA for example). To check for nonspecific binding of the primary or secondary, you can run a panel of stainings, leaving out the primary antibody or replacing with a general isotype control, as Kim suggested. Andrea Marion Graduate Student University of Illinois at Chicago Hi everybody! Maybe you can help me. I'm trying to do immunofluorescences to see the colocalisation between two proteins (PSD + Oligomeres) using Oregon Green (goat anti mouse) and Texas Red (goat anti rabbit). But my probleme, is that the analysis of the slides reveals too much colocalisation. I mean, all seems yellow, as if both fluorochromes show the same thing. It's the first time I do fluorescence, so I don't know where could come the problem. I tried to do a simple staining, 1 slide with my first antibody and its fluorochrome, and the second slide with the other first antibody with its fluorochome and then check the result with the filter red and green. But it's strange because I see both color as if I had put both fluorochromes (second antibodies). Has somebody already had this problem? What can I do to resolve it? Thank you very much for your help. Natalia From trathborne <@t> somerset-healthcare.com Tue Jan 24 14:30:43 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 24 14:30:54 2012 Subject: [Histonet] EPHI Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7ED10@SMCMAIL01.somerset-healthcare.com> I'm wondering what others do when selling used instruments that contain EPHI (electronic protected health information). Do you destroy the hard drive which also has the software programs, or is there another alternative? I have an old Dako Autostainer that we have replaced and would like to sell, but am faced with the challenge of eliminating the patient information from it. Dealers of used equipment, how do you address this problem? I expect you see it quite frequently. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From dunatrsd <@t> sbcglobal.net Tue Jan 24 14:41:16 2012 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Tue Jan 24 14:41:20 2012 Subject: [Histonet] 2012 California Society for Histotechnology Symposium in San Diego In-Reply-To: <3f2e2a196311e8ba5eb67e177d97b6c3.squirrel@webmail.uic.edu> References: <3f2e2a196311e8ba5eb67e177d97b6c3.squirrel@webmail.uic.edu> Message-ID: <1327437676.70598.YahooMailRC@web83913.mail.sp1.yahoo.com> Subject: CSH Meeting May 3-6 ? This year?s meeting will be at the Bahia resort hotel on Mission Bay.? 2 Blocks from the Ocean.? We are Presently lining up an outstanding list of lecturers.? Classes will be held on 2 paddle wheel boats.? Take a look at the hotel website and start planning now.? Details and registration will be posted soon on the Society website.? ? http://www.bahiahotel.com/ http://www.californiahistology.org/events.html ? ? The 2012 Symposium/Convention will be held in May in San Diego, CA. When: May 3 - 6, 2012 Hotel Information: Bahia Resort Hotel 998 West Mission Bay Drive, San Diego CA 858.488.0551 Reserve your room now at: https://shop.evanshotels.com/bahia_groups/casocf1205093.html ? Note: To receive the group rate of $109.00 per night please mention CSH when registering with the hotel. The cutoff date for the group rate is April 3, 2012. ? ? ? ?? James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org From kryan <@t> nfderm.com Tue Jan 24 14:50:50 2012 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Tue Jan 24 14:50:55 2012 Subject: [Histonet] EPHI In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7ED10@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711F7ED10@SMCMAIL01.somerset-healthcare.com> Message-ID: That are strict guidelines given for this on the HIPPA website. I know because somehow I was delegated to write the procedure. Just check out their website and look at "physical safeguards". Kaye Ryan North Florida Dermatology Associates, PA Lab Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 24, 2012 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EPHI I'm wondering what others do when selling used instruments that contain EPHI (electronic protected health information). Do you destroy the hard drive which also has the software programs, or is there another alternative? I have an old Dako Autostainer that we have replaced and would like to sell, but am faced with the challenge of eliminating the patient information from it. Dealers of used equipment, how do you address this problem? I expect you see it quite frequently. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkwaa <@t> bidmc.harvard.edu Tue Jan 24 17:56:54 2012 From: kkwaa <@t> bidmc.harvard.edu (kkwaa@bidmc.harvard.edu) Date: Tue Jan 24 17:56:58 2012 Subject: [Histonet] LCM on Paxgene fixed tissue Message-ID: <9oru5vvol7de0iygus61jqrg.1327449308814@email.android.com> Does anyone have any experience working with paxgene fixed tissue on membrane slides for LCM? If so - how do you deal with tissue adherence without heating slides longer. Thx Kim Donadio wrote: Oops. 10% bleach to clean. They do have some stuff called tuberculicide < spelled wrong I'm sure. Oh and get a tine test to see if you had exposure. Sorry for my half reply earlier. Was on break at work with ten things in head. Hope you havnt had an exposure Best wishes Kim Donadio Sent from my iPhone On Jan 20, 2012, at 10:06 AM, Kim Donadio wrote: > If your in a hospital: I've always had it verified with the microbiology department if it hasn't been done already(if not then still the following) Then it needs to be reported to your local department of health and the CDC since it is a reportable disease. I'm sure others can expand on this but this is what I've had to do. > Kim Donadio > > Sent from my iPhone > > On Jan 20, 2012, at 6:47 AM, Michele Email wrote: > >> Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated. >> Thank you >> Michele Carr >> >> >> Sent from my iPad >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Helen.Ilsley <@t> uct.ac.za Wed Jan 25 04:35:42 2012 From: Helen.Ilsley <@t> uct.ac.za (Helen Ilsley) Date: Wed Jan 25 04:48:54 2012 Subject: [Histonet] M1 phenotype marker Message-ID: <02DE68BE-CE01-475A-B92A-8EF5AC350026@uct.ac.za> Hi Has anyone used anti - CCK7 to differentiate M1 from M2 macrophages and what were your results like? I have a paper and am thinking of getting this antibody but would like to know if anyone else has used anti -CCK7. It will be used on rat tissue. Many thanks Helen Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6472 021-448 5935 (fax) ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From kdwyer3322 <@t> aol.com Wed Jan 25 06:12:50 2012 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Wed Jan 25 06:13:08 2012 Subject: [Histonet] Texas Society for Histotechnology 2012 Convention - Fiesta of Progress - San Antonio Texas Message-ID: <8CEA971CA85D4A0-1DAC-330B9@webmail-m099.sysops.aol.com> Hi Histonet! The TSH program is ready! If you would like an electronic copy of the program please respond to this e-mail. The TSH program will be posted on the TSH web site also at txsh.org. in a few days. Regards, TSH Convention Committee From ASelf <@t> georgetownhospitalsystem.org Wed Jan 25 06:55:49 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Jan 25 06:55:56 2012 Subject: [Histonet] Non-Gyn Cytology Specimen Staining Message-ID: Good Morning Histonetters, I am looking to speak to someone that can help me with some questions that I have about non-gyn cytology specimen staining. We only do non-gyns in house and we stain I would say about 90% of them separate because of the type of specimen it is. We have been staining the bronchial specimens together and now they are getting cross-contaminated. We do direct smears on our cytology specimens. I am looking to see how other facilities are processing and staining their non-gyn specimens. I have many questions so if you would send me your telephone number and what would be a good time to contact you I will give you a call. Thanks in advance for your help, Amy Amy Self Georgetown Hospital System Georgetown, SC 843-527-7179 (p) NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From NHeath <@t> Lifespan.org Wed Jan 25 07:17:57 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Wed Jan 25 07:18:02 2012 Subject: [Histonet] RISH's Carnival Mardi Gras Conference 3-24-2012 Message-ID: <130E8991F210424096EFC6F42EA33B24088DA965@LSCOEXCH1.lsmaster.lifespan.org> Hi Everyone, The Rhode Island Society for Histotechnology will be hosting their "Carnival Mardi Gras Conference" on Saturday March 24 2012 at Dave & Buster's in the Providence Place Mall, Providence, RI. This is a one day conference providing 7 CEU's. Freida Carson is our guest speaker! To register and for more details please visit "RISH's Mardi Gras 2012" page on our website at www.rihisto.org FREE RISH membership is included with conference registration! "Laissez les bons temps rouler" Nancy Heath, HT (ASCP) President - Rhode Island Society for Histotechnology president@rihisto.org phone: 401-444-3246 From arunjyothisp <@t> gmail.com Wed Jan 25 07:25:10 2012 From: arunjyothisp <@t> gmail.com (Arun Jyothi S.P) Date: Wed Jan 25 07:25:20 2012 Subject: [Histonet] Reg: Tissue is hard to cut after processing Message-ID: Dear all, We are having a hard time in cutting blocks. All our blocks especially uterus, prostate chips etc became very hard after processing, we cant even trim the blocks its very hard. we use SLEE MTM automated tissue processor and our schedule is 1, Formalin - 1hr - ambient - 40 degree 2, Formalin - 2 hr - vacuum - 40 degree 3, 70% ethanol - 1hr - vacuum - Room temp 4, 95% ethanol - 1hr - vacuum - Room temp 5 and 6- 100% ethanol - 1hr each - vacuum - room temp 7, 100% ethanol - 1.30 hr - vacuum - room temp 8, 9 and 10 - Xylene - 1 hr each - vacuum - 30, 35, 40 degree respectively 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. We use Merck paraffin wax of 56 degree M.P Is there any problem with tissue processing schedule or something else is wrong? We are in a big problem really expecting some valuable suggestion and advices. with regards ARUN JYOTHI S.P. Histotechnologist United Laboratories Co. Kuwait From melissa <@t> alliedsearchpartners.com Wed Jan 25 09:05:11 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed Jan 25 09:05:19 2012 Subject: [Histonet] Histology, IHC, Grossing, Cytoprep Job Openings in New York Message-ID: Allied Search Partners has been retained for the following searches. We have openings in Histology, IHC, Grossing, and Cytoprep. Please forward this along to anyone who you know that would be interested in any of the following positions. We do offer a referral bonus. 1. Please email a copy of updated resume to melissa@alliedsearchpartners.com for a full job description. We have the following positions available: 1. Immunohistochemistry (IHC) Histotech (2 positions available) LOCATION: Port Chester, NY area DEPARTMENT & SCHEDULE: Position #1: Monday-Friday 5pm-1:30am Position #2: Monday-Friday 9pm-5:30am 1. Histotech LOCATION: New York, NY SCHEDULE & DEPARTMENT: Monday-Friday 12am (midnight)-8am 1. Cytopreparatory Technician LOCATION: Port Chester, NY area SCHEDULE & DEPARTMENT: Monday-Friday 10am-6:30am 1. Grossing Technician LOCATION: Port Chester, NY area SCHEDULE & DEPARTMENT Monday-Friday Overnight Shift/3rd shift (exact hours are not determined) Monday-Friday Second Shift (exact hours are not determined) -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From algranth <@t> email.arizona.edu Wed Jan 25 09:22:07 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jan 25 09:22:20 2012 Subject: [Histonet] Reg: Tissue is hard to cut after processing In-Reply-To: References: Message-ID: Arun, It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. Andi Grantham On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: > Dear all, > > We are having a hard time in cutting blocks. All our blocks especially > uterus, prostate chips etc became very hard after processing, we cant even > trim the blocks its very hard. > we use SLEE MTM automated tissue processor > and our schedule is > 1, Formalin - 1hr - ambient - 40 degree > 2, Formalin - 2 hr - vacuum - 40 degree > 3, 70% ethanol - 1hr - vacuum - Room temp > 4, 95% ethanol - 1hr - vacuum - Room temp > 5 and 6- 100% ethanol - 1hr each - vacuum - room temp > 7, 100% ethanol - 1.30 hr - vacuum - room temp > 8, 9 and 10 - Xylene - 1 hr each - vacuum - 30, 35, 40 > degree respectively > 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. > > We use Merck paraffin wax of 56 degree M.P > > Is there any problem with tissue processing schedule or something else is > wrong? > > We are in a big problem really expecting some valuable suggestion and > advices. > > with regards > > > > ARUN JYOTHI S.P. > Histotechnologist > United Laboratories Co. > Kuwait > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbreeden <@t> nmda.nmsu.edu Wed Jan 25 09:37:29 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 25 09:37:47 2012 Subject: [Histonet] Interview Questions Message-ID: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this "fortunate" before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with "meat" to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Lynn.Burton <@t> Illinois.gov Wed Jan 25 09:42:56 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jan 25 09:43:35 2012 Subject: [Histonet] RE: Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC006483C075B@IL084EXMBX214.illinois.gov> I don't have any ideas but I am sorry to hear you are leaving. Good Luck with your future. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Wednesday, January 25, 2012 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this "fortunate" before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with "meat" to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Jan 25 10:10:06 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jan 25 10:10:14 2012 Subject: [Histonet] RE: Interview Questions In-Reply-To: <4A6E2CACA1E017408EBA1B9911952CC006483C075B@IL084EXMBX214.illinois.gov> References: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> <4A6E2CACA1E017408EBA1B9911952CC006483C075B@IL084EXMBX214.illinois.gov> Message-ID: <1327507806.39977.YahooMailNeo@web130105.mail.mud.yahoo.com> I had a great question asked of me when I was interviewed for my current position: ? Tell me of a time that you had to rescue some tissue; what happened to it that ruined it and what did you do to fix it? ? A great question for a histologist.? People with?experience in the field should have several stories related to this question (especially in research, when you have PIs collecting tissue for you that often have no clue what they are doing)!!! ? ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ? ? ? ? ________________________________ From: "Burton, Lynn" To: "Breeden, Sara" ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 25, 2012 10:42 AM Subject: [Histonet] RE: Interview Questions I don't have any ideas but I am sorry to hear you are leaving. Good Luck with your future. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Wednesday, January 25, 2012 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement).? I've not been this "fortunate" before and I do know there are questions one cannot ask so that's not an issue.? What I'd like to know is what I SHOULD ask.? This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential).? What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with "meat" to them.? Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Jan 25 10:11:14 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jan 25 10:11:21 2012 Subject: [Histonet] neutrophil marker for FFPE mouse tissue Message-ID: <1327507874.8565.YahooMailNeo@web130104.mail.mud.yahoo.com> Hello! What antibodies are people using to stain for neutrophils in FFPE mouse tissue? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From liz <@t> premierlab.com Wed Jan 25 10:17:05 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jan 25 10:17:10 2012 Subject: [Histonet] neutrophil marker for FFPE mouse tissue In-Reply-To: <1327507874.8565.YahooMailNeo@web130104.mail.mud.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC55E1@SBS2K8.premierlab.local> Kim Serotec has a nice antibody that works on FFPE samples and also Formic acid decal samples. Here is the info: Protocol: Mouse Neutrophil Immunohistochemical Stain (Enzyme) Clone: 7/4 Vendor: Serotec Catalog Number: MCA771 Species: Mouse Isotype: Rat IgG2a Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, January 25, 2012 9:11 AM To: Histonet Subject: [Histonet] neutrophil marker for FFPE mouse tissue Hello! What antibodies are people using to stain for neutrophils in FFPE mouse tissue? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From candice_camille <@t> yahoo.com Wed Jan 25 10:21:12 2012 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jan 25 10:21:21 2012 Subject: [Histonet] Oil red O versus Sudan 4 Message-ID: <1327508472.83402.YahooMailNeo@web125403.mail.ne1.yahoo.com> Hi Histonetters ? ? I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and? then i stain the inside of the aorta for my plaques. ? This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. ? My question is what is the difference and what should I do differently with the Oil red o? ? Thanks so much for your help! I remain yours truely, Candice Camille From DKBoyd <@t> chs.net Wed Jan 25 10:21:55 2012 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jan 25 10:23:40 2012 Subject: [Histonet] Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: First of all, DON'T ASSUME ANYTHING. Ask questions about every aspect of the position. Let them know what they will be responsible for. Look for desired qualities ie: detail oriented, high work standard, team worker, flexible, multitasker, critical thinker, acceptable to constructive criticism, good verbal communication, etc. Interview Questions I use are: Are you proficient with frozen sections? Are you willing to work over occasionally to perform frozen sections? What are your interest or hobbies? Where are your professional goals. Where do you see yourself in 5 years. I always ask one critical thinking question about processing to test their knowledge. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Breeden, Sara" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/25/2012 10:41 AM To cc Subject [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this "fortunate" before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with "meat" to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From joelleweaver <@t> hotmail.com Wed Jan 25 10:25:00 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 25 10:25:05 2012 Subject: [Histonet] Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Here are some idea starters..http://www.negotiations.com/articles/top-interview-questions/ maybe you can adapt it to your particular job role, the culture and people that you were working with directly, or what you found most challenging/frustrating/inspiring? How interesting to be interviewing your replacement!Joelle Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Wed, 25 Jan 2012 08:37:29 -0700 > From: sbreeden@nmda.nmsu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > Okay, My People - I will be one of the interviewers for locating my > replacement). I've not been this "fortunate" before and I do know there > are questions one cannot ask so that's not an issue. What I'd like to > know is what I SHOULD ask. This position is fairly straightforward - > basic veterinary histology with nothing significantly challenging (but > with that potential). What would YOU want to know about a candidate > that would convince you that this person was The One? I need questions > with "meat" to them. Your suggestions will be much-ly appreciated. > Gracias! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jm.lapointe <@t> accellab.com Wed Jan 25 10:40:29 2012 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Wed Jan 25 10:40:37 2012 Subject: [Histonet] Antibody for canine IgG In-Reply-To: <201201121805.q0CI5KSU003175@gateway5.lastspam.com> References: <201201121805.q0CI5KSU003175@gateway5.lastspam.com> Message-ID: I'm looking for an antibody that works for canine IgG, to be used for FFPE tissue IHC, and which should react with both IgG1 and IgG2. Ideally mouse monoclonal. Does not have to be specific to dog, as long as it reacts with it adequately in IHC. Any recommendations ? _____________________ Jean-Martin Lapointe AccelLAB Inc From liz <@t> premierlab.com Wed Jan 25 10:43:22 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jan 25 10:43:34 2012 Subject: [Histonet] Oil red O versus Sudan 4 In-Reply-To: <1327508472.83402.YahooMailNeo@web125403.mail.ne1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC55E2@SBS2K8.premierlab.local> Candice Most of the references regarding this technique use Sudan IV, that's what we use here. We use Oil Red O for the Aortic Root Sections but not for the En Face Analysis of the Aorta. I have the Sudan IV procedure we use here if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 25, 2012 9:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and then i stain the inside of the aorta for my plaques. This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. My question is what is the difference and what should I do differently with the Oil red o? Thanks so much for your help! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Jan 25 10:49:09 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jan 25 10:49:22 2012 Subject: [Histonet] RELIA Solutions - Hot Histology Job Alert Here are my newest and most exciting opportunities. Take a look!! Message-ID: <6E7F42D574E8461B9383D130DB566B00@ownerf1abaad51> Hi Histonetters!! How are you doing today? I have a few new positions to run by you. These are with some great companies that are offering excellent compensation, benefits and relocation/sign on bonuse. If you are interested contact me at relia1@earthlink.net or toll free at 866-607-3542. Here is the list: =>Lead Histotech - GI - Kissimmee, FL - Florida license required. This is an in office pathology lab and you would be the sole practitioner. =>Night Shift Histology Supervisor - Miami, FL Florida tech or supervisor license is required - opportunity for advancement!! =>Histology Tech - Day Shift New York NYS license required. positions in Long Island, Suffern and Rochester. =>Histology Tech - Strong Dermpath - Lancaster, PA =>Grossing Histotech - Nights, Wallingford, CT =>Grossing Histotech - Nights Chattanooga, TN **** I also need a NY licensed Cytotech for a lab on Long Island - If you know anybody over in the cyto department that might be interested I do pay a referral bonus of $500.00 :) Have a great Hump Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From sbreeden <@t> nmda.nmsu.edu Wed Jan 25 10:52:08 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 25 10:52:21 2012 Subject: [Histonet] Interview Questions Message-ID: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was..... (wait for it....) "How do you feel about personal phone calls?". Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From candice_camille <@t> yahoo.com Wed Jan 25 10:55:43 2012 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jan 25 10:56:50 2012 Subject: [Histonet] Oil red O versus Sudan 4 In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5001CF3B8E@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <1327508472.83402.YahooMailNeo@web125403.mail.ne1.yahoo.com> <8A70A9B2ECDD084DACFE6C59FCF86D5001CF3B8E@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <1327510543.59460.YahooMailNeo@web125406.mail.ne1.yahoo.com> Hi Sarah ? ?Actually, There is no micotmy involved. We biopsy the aorta. Clean the surrounding fat, cut it down the middle with a blade. And then we stain with Sudan 4. The aorta is mounted whole on a wax plate. we hydrate the tissue with PBS before we stain. This worked fine with the Sudan 4. Should I discontinue hydrating with PBS and use water instead with the Oil Red O. ? Also with the Oil red O, we were rinsing them with isopranol before we stained them. Could this be the problem? I remain yours truely, Candice Camille ________________________________ From: Sarah Dysart To: Candice Smoots Sent: Wednesday, January 25, 2012 10:32 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 I do oil red a lot.? You are doing it on frozen sections right? What are you coverslipping with? Need a few more details of the protocol and I might be able to help =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 25, 2012 10:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters ? ? I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and? then i stain the inside of the aorta for my plaques. ? This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. ? My question is what is the difference and what should I do differently with the Oil red o? ? Thanks so much for your help! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From candice_camille <@t> yahoo.com Wed Jan 25 10:57:44 2012 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jan 25 10:57:51 2012 Subject: [Histonet] Oil red O versus Sudan 4 In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC55E2@SBS2K8.premierlab.local> References: <1327508472.83402.YahooMailNeo@web125403.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE011390CC55E2@SBS2K8.premierlab.local> Message-ID: <1327510664.60736.YahooMailNeo@web125406.mail.ne1.yahoo.com> That would be great! ? Thanks I remain yours truely, Candice Camille ________________________________ From: Elizabeth Chlipala To: 'Candice Smoots' ; Histonet Sent: Wednesday, January 25, 2012 10:43 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 Candice Most of the references regarding this technique use Sudan IV, that's what we use here. We use Oil Red O for the Aortic Root Sections but not for the En Face Analysis of the Aorta.? I have the Sudan IV procedure we use here if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 25, 2012 9:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and? then i stain the inside of the aorta for my plaques. This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. My question is what is the difference and what should I do differently with the Oil red o? Thanks so much for your help! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Wed Jan 25 10:59:02 2012 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Jan 25 10:59:12 2012 Subject: [Histonet] RE: Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A002718833236@CVM76.vetmed.wsu.edu> Hi Sally, I think I would ask if they were familiar with the terms bovine, porcine, ovine, caprine, equine, ect. Then ask if they would process bovine tissue differently than mouse tissues. Ask what kind of problems they might expect to encounter doing IHC and immunofluorescence on animal tissue. If you handle legal cases, ask questions about tissue identity and chain of evidence. Ask questions on lab safety and chemical waste disposal. Good luck, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 7:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this "fortunate" before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with "meat" to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Jan 25 11:19:05 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Jan 25 11:19:12 2012 Subject: [Histonet] Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> It would seem that questions like "How do you feel about cannibalism?" might also be out but might be far more helpful; than "phone" questions. On the serious side, when I was much younger I hired a person who was able to answer all the right "histo" questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as "Dr." He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was..... (wait for it....) "How do you feel about personal phone calls?". Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From mtitford <@t> aol.com Wed Jan 25 11:46:43 2012 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jan 25 11:46:53 2012 Subject: [Histonet] Ceramic knives for autopsies? Message-ID: <8CEA9A06F34247C-DAC-3626A@webmail-m057.sysops.aol.com> Has anyone had any experience with using the new ceramic knives for autopsies? Where do you purchase them? Do they hold their edge? Can they be resharpened? Thank you Michael Titford USA Pathology Mobile AL USA From joelleweaver <@t> hotmail.com Wed Jan 25 11:48:34 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 25 11:48:42 2012 Subject: [Histonet] Interview Questions In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu>, <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> Message-ID: Good point about "personality" questions. I have often had this experience, where I was leaving the meeting wondering about the place from too much time spent on this sort of thing. However, I think that some line of questioning for this information is good to try to see if you can learn a little about everyone's general temperment - though I do concede this is difficult in such a staged interaction as an interview. Sometimes people have knowledge and technical skills, but are very confrontational, poor communicators, or have other attributes which make them a bad fit for any particular organization, and sometimes these things end up "sinking the ship" so to speak as far as the employee-employer relationship, even when skills, reference or credentials are there. Everything about an interview is pretty much a calculated risk I suppose. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Wed, 25 Jan 2012 10:19:05 -0700 > From: billodonnell@catholichealth.net > To: sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > CC: > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at a > local university with a lab coat that listed him as "Dr." He had indeed > worked in a histo lab, but as a lab assistant, and so the the > understanding of what a histologist does was well rehearsed. (BTW, it > topok me about two weeks to catch on, though the more experienced techs > in the department figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them > were particularly useful or telling. For inistance, they would not ask > if the person was an histo tech, but would simply ask, did he indeed > work at your institution? > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done as > well weeding them out by histo questions. I'm sure your HR folks will > do a fine job of this. > > Also, once you have determined that they actually have the skills, or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in the > mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, > Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jan 25 11:47:01 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 25 11:50:02 2012 Subject: [Histonet] Interview Questions In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like "How do you feel about cannibalism?" might also be out but might be far more helpful; than "phone" questions. On the serious side, when I was much younger I hired a person who was able to answer all the right "histo" questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as "Dr." He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was..... (wait for it....) "How do you feel about personal phone calls?". Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From joelleweaver <@t> hotmail.com Wed Jan 25 11:52:43 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 25 11:52:47 2012 Subject: [Histonet] Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: How did you answer?! Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Wed, 25 Jan 2012 09:52:08 -0700 > From: sbreeden@nmda.nmsu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jan 25 12:02:39 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 25 12:02:49 2012 Subject: [Histonet] Interview Questions In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu>, <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net>, <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> Message-ID: Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: trathborne@somerset-healthcare.com > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu > Date: Wed, 25 Jan 2012 17:47:01 +0000 > Subject: RE: [Histonet] Interview Questions > CC: > > If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? > What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). > People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. > Good luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > Sent: Wednesday, January 25, 2012 12:19 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was able to answer all the right "histo" questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as "Dr." He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? > > The place where I worked required little or nothing for proof of experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. > > Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Jan 25 12:09:13 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed Jan 25 12:09:24 2012 Subject: [Histonet] Interview Questions In-Reply-To: References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> Message-ID: Just to be devil's advocate here...... asking a person to "prove" their skills - what happens, if through nervousness, or being unfamiliar with the equipment, they injure themselves. Where does the liability lie? Rather ask questions regarding cutting speed, way in which tissue is embedded etc, and review the person's skill during an agreed probationary period. If they are not what u expected, then you can get rid of them..... ... On Wed, Jan 25, 2012 at 8:02 PM, joelle weaver wrote: > > Love this! I always want to do demonstration during technical interviews, > but usually get "shot down" from managers and argued with in general, as > in people don't feel that they should have to "prove" they can do > histology. This perception, I never got, because I always saw it as in a > job interview-in what other situation are you more trying to "prove" or > impress with your knowledge, attitude, skills and experience? If you do > bench work, you can tell in just a few minutes of observation much more > information than you could get with quite a few questions. To be fair, I > take into account nervousness, being closely observed, and lack of > familiarity with equipment etc. I don't know, I think its fair if those are > important skills to the position/role. Was not sure if Sara's job was > mostly technical though, so thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathborne@somerset-healthcare.com > > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > > Date: Wed, 25 Jan 2012 17:47:01 +0000 > > Subject: RE: [Histonet] Interview Questions > > CC: > > > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at a > multi-head scope and review slides with them? > > What will the person be responsible for? Do they have experience with > all of these tasks? What would they do in a crisis situation (you can make > up one yourself that would be plausible). > > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on in > their personal lives. Be careful with how you ask these questions though. > Your HR department should be able to give you guidance in how to phrase > things. > > Good luck. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > > Sent: Wednesday, January 25, 2012 12:19 PM > > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Interview Questions > > > > It would seem that questions like "How do you feel about cannibalism?" > > might also be out but might be far more helpful; than "phone" questions. > > > > > > On the serious side, when I was much younger I hired a person who was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at a > local university with a lab coat that listed him as "Dr." He had indeed > worked in a histo lab, but as a lab assistant, and so the the understanding > of what a histologist does was well rehearsed. (BTW, it topok me about two > weeks to catch on, though the more experienced techs in the department > figured it out almost right away) > > > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them were > particularly useful or telling. For inistance, they would not ask if the > person was an histo tech, but would simply ask, did he indeed work at your > institution? > > > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > > > Today, however, reference checking is a lot easier and more reliable. > > > > I guess my point here is that a good reference check needs to be done as > well weeding them out by histo questions. I'm sure your HR folks will do a > fine job of this. > > > > Also, once you have determined that they actually have the skills, or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in the > mind of the applicant. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > > Sent: Wednesday, January 25, 2012 10:52 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Interview Questions > > > > So far, I am TOTALLY impressed and so grateful for your suggestions. > > And here's why... did I ever tell anyone out there what the FIRST > > question I was asked by the pathologist at my interview? It was..... > > (wait for it....) > > > > > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > > I sure don't want someone to remember ME that way!!! > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical > Center > > and are intended only for the addressee. The information contained in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From POWELL_SA <@t> mercer.edu Wed Jan 25 12:15:48 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Jan 25 12:16:01 2012 Subject: [Histonet] Interview Questions In-Reply-To: References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD78838CC@MERCERMAIL.MercerU.local> Make them sign a non-liability clause before doing the test? You need to know if they can do the work before hiring, not after, nervous or not, and not how well they answer questions. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Wednesday, January 25, 2012 1:09 PM To: Histonet Subject: Re: [Histonet] Interview Questions Just to be devil's advocate here...... asking a person to "prove" their skills - what happens, if through nervousness, or being unfamiliar with the equipment, they injure themselves. Where does the liability lie? Rather ask questions regarding cutting speed, way in which tissue is embedded etc, and review the person's skill during an agreed probationary period. If they are not what u expected, then you can get rid of them..... ... On Wed, Jan 25, 2012 at 8:02 PM, joelle weaver wrote: > > Love this! I always want to do demonstration during technical interviews, > but usually get "shot down" from managers and argued with in general, as > in people don't feel that they should have to "prove" they can do > histology. This perception, I never got, because I always saw it as in a > job interview-in what other situation are you more trying to "prove" or > impress with your knowledge, attitude, skills and experience? If you do > bench work, you can tell in just a few minutes of observation much more > information than you could get with quite a few questions. To be fair, I > take into account nervousness, being closely observed, and lack of > familiarity with equipment etc. I don't know, I think its fair if those are > important skills to the position/role. Was not sure if Sara's job was > mostly technical though, so thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathborne@somerset-healthcare.com > > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > > Date: Wed, 25 Jan 2012 17:47:01 +0000 > > Subject: RE: [Histonet] Interview Questions > > CC: > > > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at a > multi-head scope and review slides with them? > > What will the person be responsible for? Do they have experience with > all of these tasks? What would they do in a crisis situation (you can make > up one yourself that would be plausible). > > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on in > their personal lives. Be careful with how you ask these questions though. > Your HR department should be able to give you guidance in how to phrase > things. > > Good luck. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > > Sent: Wednesday, January 25, 2012 12:19 PM > > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Interview Questions > > > > It would seem that questions like "How do you feel about cannibalism?" > > might also be out but might be far more helpful; than "phone" questions. > > > > > > On the serious side, when I was much younger I hired a person who was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at a > local university with a lab coat that listed him as "Dr." He had indeed > worked in a histo lab, but as a lab assistant, and so the the understanding > of what a histologist does was well rehearsed. (BTW, it topok me about two > weeks to catch on, though the more experienced techs in the department > figured it out almost right away) > > > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them were > particularly useful or telling. For inistance, they would not ask if the > person was an histo tech, but would simply ask, did he indeed work at your > institution? > > > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > > > Today, however, reference checking is a lot easier and more reliable. > > > > I guess my point here is that a good reference check needs to be done as > well weeding them out by histo questions. I'm sure your HR folks will do a > fine job of this. > > > > Also, once you have determined that they actually have the skills, or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in the > mind of the applicant. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > > Sent: Wednesday, January 25, 2012 10:52 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Interview Questions > > > > So far, I am TOTALLY impressed and so grateful for your suggestions. > > And here's why... did I ever tell anyone out there what the FIRST > > question I was asked by the pathologist at my interview? It was..... > > (wait for it....) > > > > > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > > I sure don't want someone to remember ME that way!!! > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical > Center > > and are intended only for the addressee. The information contained in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jan 25 12:24:20 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 25 12:24:29 2012 Subject: [Histonet] Interview Questions In-Reply-To: <9BF995BC0E47744E9673A41486E24EE24AD78838CC@MERCERMAIL.MercerU.local> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu>, <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net>, <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com>, , , <9BF995BC0E47744E9673A41486E24EE24AD78838CC@MERCERMAIL.MercerU.local> Message-ID: I guess someone could get hurt. I had to stop someone once, either they were unbelievably nervous, or had not used a microtome in QUITE some time, and I thought they might hurt themselves. I stopped the activity, but legalities might prevail, a consent could suffice to cover for this maybe. Probation is good, if it is enforced. Have seen people that I was not sure how they made it through that period, so I guess the weight that is given varies. All in all, a challenge to locate, recruit, screen, hire and retain good people! Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: POWELL_SA@mercer.edu > To: louise.renton@gmail.com > Date: Wed, 25 Jan 2012 13:15:48 -0500 > Subject: RE: [Histonet] Interview Questions > CC: histonet@lists.utsouthwestern.edu > > Make them sign a non-liability clause before doing the test? You need to know if they can do the work before hiring, not after, nervous or not, and not how well they answer questions. > > sp > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton > Sent: Wednesday, January 25, 2012 1:09 PM > To: Histonet > Subject: Re: [Histonet] Interview Questions > > Just to be devil's advocate here...... > > asking a person to "prove" their skills - what happens, if through > nervousness, or being unfamiliar with the equipment, they injure > themselves. Where does the liability lie? > > Rather ask questions regarding cutting speed, way in which tissue is > embedded etc, and review the person's skill during an agreed probationary > period. If they are not what u expected, then you can get rid of them..... > ... > On Wed, Jan 25, 2012 at 8:02 PM, joelle weaver wrote: > > > > > Love this! I always want to do demonstration during technical interviews, > > but usually get "shot down" from managers and argued with in general, as > > in people don't feel that they should have to "prove" they can do > > histology. This perception, I never got, because I always saw it as in a > > job interview-in what other situation are you more trying to "prove" or > > impress with your knowledge, attitude, skills and experience? If you do > > bench work, you can tell in just a few minutes of observation much more > > information than you could get with quite a few questions. To be fair, I > > take into account nervousness, being closely observed, and lack of > > familiarity with equipment etc. I don't know, I think its fair if those are > > important skills to the position/role. Was not sure if Sara's job was > > mostly technical though, so thought I might keep it general. > > > > Joelle Weaver MAOM, (HTL) ASCP > > > > http://www.linkedin.com/in/joelleweaver > > > > > From: trathborne@somerset-healthcare.com > > > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > > histonet@lists.utsouthwestern.edu > > > Date: Wed, 25 Jan 2012 17:47:01 +0000 > > > Subject: RE: [Histonet] Interview Questions > > > CC: > > > > > > If your replacement will be doing actual histology, will your > > institution permit the applicant to embed and cut? Can you sit down at a > > multi-head scope and review slides with them? > > > What will the person be responsible for? Do they have experience with > > all of these tasks? What would they do in a crisis situation (you can make > > up one yourself that would be plausible). > > > People who volunteer in their personal lives, may do the same at work. > > Ask how they juggle their schedule though, if there is a lot going on in > > their personal lives. Be careful with how you ask these questions though. > > Your HR department should be able to give you guidance in how to phrase > > things. > > > Good luck. > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > > > Sent: Wednesday, January 25, 2012 12:19 PM > > > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > > > Subject: RE: [Histonet] Interview Questions > > > > > > It would seem that questions like "How do you feel about cannibalism?" > > > might also be out but might be far more helpful; than "phone" questions. > > > > > > > > > On the serious side, when I was much younger I hired a person who was > > able to answer all the right "histo" questions and so I hired him. He > > turned out to be a poser, who, shortly after I fired him showed up at a > > local university with a lab coat that listed him as "Dr." He had indeed > > worked in a histo lab, but as a lab assistant, and so the the understanding > > of what a histologist does was well rehearsed. (BTW, it topok me about two > > weeks to catch on, though the more experienced techs in the department > > figured it out almost right away) > > > > > > To be fair, it was during a time in hiring history when HR departments > > were not willing to give useful reference data and there were only a > > handful of questions they would even ask when checking. None of them were > > particularly useful or telling. For inistance, they would not ask if the > > person was an histo tech, but would simply ask, did he indeed work at your > > institution? > > > > > > The place where I worked required little or nothing for proof of > > experience. There was no background check either. > > > > > > Today, however, reference checking is a lot easier and more reliable. > > > > > > I guess my point here is that a good reference check needs to be done as > > well weeding them out by histo questions. I'm sure your HR folks will do a > > fine job of this. > > > > > > Also, once you have determined that they actually have the skills, or a > > realistic potential of gaining them, questions concerning dynamics of > > interaction are appropriate, though may lead to wrong impressions in the > > mind of the applicant. > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Breeden, Sara > > > Sent: Wednesday, January 25, 2012 10:52 AM > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Interview Questions > > > > > > So far, I am TOTALLY impressed and so grateful for your suggestions. > > > And here's why... did I ever tell anyone out there what the FIRST > > > question I was asked by the pathologist at my interview? It was..... > > > (wait for it....) > > > > > > > > > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > > > I sure don't want someone to remember ME that way!!! > > > > > > > > > > > > Sally Breeden, HT(ASCP) > > > > > > New Mexico Department of Agriculture > > > > > > Veterinary Diagnostic Services > > > > > > 1101 Camino de Salud NE > > > > > > Albuquerque, NM 87102 > > > > > > 505-383-9278 (Histology Lab) > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > This electronic mail and any attached documents are intended solely for > > the named addressee(s) and contain confidential information. If you are not > > an addressee, or responsible for delivering this email to an addressee, you > > have received this email in error and are notified that reading, copying, > > or disclosing this email is prohibited. If you received this email in > > error, immediately reply to the sender and delete the message completely > > from your computer system. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > CONFIDENTIALITY NOTICE > > > This message and any included attachments are from Somerset Medical > > Center > > > and are intended only for the addressee. The information contained in > > this > > > message is confidential and may contain privileged, confidential, > > > proprietary and/or trade secret information entitled to protection and/or > > > exemption from disclosure under applicable law. Unauthorized forwarding, > > > printing, copying, distribution, or use of such information is strictly > > > prohibited and may be unlawful. If you are not the addressee, please > > > promptly delete this message and notify the sender of the delivery error > > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > > at 908-685-2200, ext. 4050. > > > > > > Be sure to visit Somerset Medical Center's Web site - > > > www.somersetmedicalcenter.com - for the most up-to-date news, > > > event listings, health information and more. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > Question: Are rhinos overweight unicorns? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Jan 25 12:26:33 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Wed Jan 25 12:26:38 2012 Subject: [Histonet] Re: Interview questions Message-ID: <9F3CFEE76E51B64991C7485270890B4009EF1A6D@EX5.lj.gnf.org> Sally, My answer to that question is "I *love* personal phone calls! I just think they are inappropriate in a workplace and should be minimized whenever possible." Do not ask yes or no questions. Keep them open ended. Don't ignore red flags that arise, even if you can't explain why it's a cause for concern. Sometimes you just have to go with your gut. Ask them what they think they can contribute that is unique from the other candidates you are interviewing. Ask them to describe their perfect job. You can get a lot of information from those two questions, more even than the answers at face value. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From raestask <@t> grics.net Wed Jan 25 12:35:31 2012 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Wed Jan 25 12:35:52 2012 Subject: [Histonet] H Pylori Control Message-ID: Good Afternoon everyone, My institution is about to start running H Pylori for another lab. They microwave process their tissue. Would there be anyone out there who microwave processes and would have one or two blocks of H Pylori positive control tissue they would be willing to let us have? Thanks in advance, Rae Ann Staskiewicz Methodist Medical Center of Illinois Peoria, IL From crochieresteve <@t> aol.com Wed Jan 25 12:43:09 2012 From: crochieresteve <@t> aol.com (Steven) Date: Wed Jan 25 12:43:13 2012 Subject: [Histonet] (no subject) Message-ID: <8CEA9A851482093-87C-34D49@webmail-m016.sysops.aol.com> http://lizziethealienchild.com/wp-content/plugins/extended-comment-options/tndkrno.html From renafail2 <@t> gmail.com Wed Jan 25 12:54:21 2012 From: renafail2 <@t> gmail.com (Rena Fail) Date: Wed Jan 25 12:54:27 2012 Subject: [Histonet] Xylene and Preggers In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5001CF353C@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5001CF353C@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: Human resources ultimately represents the company . A pregnant employee should be wearing ppe when handling xylene. MSDS forms are accessible to all employees, just a click away on the computer. I can understand wanting to keep some things private until you are ready to communicate them, but when you work with chemicals such as xylene and you are pregnant making sure you are wearing the proper PPE and the air quality meets OSHA standards comes first. I don't think the information communicated by HR to your supervisors should become common knowledge, but there is an obligation to protect the employee as well as the employer, Rena Fail On Mon, Jan 23, 2012 at 12:26 PM, Sarah Dysart wrote: > Hey all, so 2 things... > > A. Does anyone have anything saying that .75% (yes less than 1) is > an acceptable exposure limit for a pregnant person and > > B. Does HR have the right to tell people that you are pregnant after > you ask them questions (ie. Your manager, and all the way up??) > > Thanks > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Wed Jan 25 13:31:09 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 25 13:31:21 2012 Subject: [Histonet] Interview Questions In-Reply-To: <9BF995BC0E47744E9673A41486E24EE24AD78838CC@MERCERMAIL.MercerU.local> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu> <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net> <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> <9BF995BC0E47744E9673A41486E24EE24AD78838CC@MERCERMAIL.MercerU.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7EFF1@SMCMAIL01.somerset-healthcare.com> Your company will have to invest a lot of money to hire the person you choose. Background check and physical to start with. Then a "training period". If you could have known during practical session that the applicant would not measure up to the needs of the department, you will save yourself time (for training), and HR (financial). Also, if the person gave up a job to take yours, and was terminated after the probationary period, that leaves them without a job too. Not a good scenario for either side. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, January 25, 2012 1:16 PM To: Louise Renton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions Make them sign a non-liability clause before doing the test? You need to know if they can do the work before hiring, not after, nervous or not, and not how well they answer questions. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Wednesday, January 25, 2012 1:09 PM To: Histonet Subject: Re: [Histonet] Interview Questions Just to be devil's advocate here...... asking a person to "prove" their skills - what happens, if through nervousness, or being unfamiliar with the equipment, they injure themselves. Where does the liability lie? Rather ask questions regarding cutting speed, way in which tissue is embedded etc, and review the person's skill during an agreed probationary period. If they are not what u expected, then you can get rid of them..... ... On Wed, Jan 25, 2012 at 8:02 PM, joelle weaver wrote: > > Love this! I always want to do demonstration during technical > interviews, but usually get "shot down" from managers and argued with > in general, as in people don't feel that they should have to "prove" > they can do histology. This perception, I never got, because I always > saw it as in a job interview-in what other situation are you more > trying to "prove" or impress with your knowledge, attitude, skills and > experience? If you do bench work, you can tell in just a few minutes > of observation much more information than you could get with quite a > few questions. To be fair, I take into account nervousness, being > closely observed, and lack of familiarity with equipment etc. I don't > know, I think its fair if those are important skills to the > position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathborne@somerset-healthcare.com > > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > > Date: Wed, 25 Jan 2012 17:47:01 +0000 > > Subject: RE: [Histonet] Interview Questions > > CC: > > > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at > a multi-head scope and review slides with them? > > What will the person be responsible for? Do they have experience > > with > all of these tasks? What would they do in a crisis situation (you can > make up one yourself that would be plausible). > > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on > in their personal lives. Be careful with how you ask these questions though. > Your HR department should be able to give you guidance in how to > phrase things. > > Good luck. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, > Bill > > Sent: Wednesday, January 25, 2012 12:19 PM > > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Interview Questions > > > > It would seem that questions like "How do you feel about cannibalism?" > > might also be out but might be far more helpful; than "phone" questions. > > > > > > On the serious side, when I was much younger I hired a person who > > was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at > a local university with a lab coat that listed him as "Dr." He had > indeed worked in a histo lab, but as a lab assistant, and so the the > understanding of what a histologist does was well rehearsed. (BTW, it > topok me about two weeks to catch on, though the more experienced > techs in the department figured it out almost right away) > > > > To be fair, it was during a time in hiring history when HR > > departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them > were particularly useful or telling. For inistance, they would not ask > if the person was an histo tech, but would simply ask, did he indeed > work at your institution? > > > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > > > Today, however, reference checking is a lot easier and more reliable. > > > > I guess my point here is that a good reference check needs to be > > done as > well weeding them out by histo questions. I'm sure your HR folks will > do a fine job of this. > > > > Also, once you have determined that they actually have the skills, > > or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in > the mind of the applicant. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > > Sent: Wednesday, January 25, 2012 10:52 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Interview Questions > > > > So far, I am TOTALLY impressed and so grateful for your suggestions. > > And here's why... did I ever tell anyone out there what the FIRST > > question I was asked by the pathologist at my interview? It was..... > > (wait for it....) > > > > > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > > I sure don't want someone to remember ME that way!!! > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely > > for > the named addressee(s) and contain confidential information. If you > are not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical > Center > > and are intended only for the addressee. The information contained > > in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection > > and/or exemption from disclosure under applicable law. Unauthorized > > forwarding, printing, copying, distribution, or use of such > > information is strictly prohibited and may be unlawful. If you are > > not the addressee, please promptly delete this message and notify > > the sender of the delivery error by e-mail or you may call Somerset > > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, event > > listings, health information and more. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From amario3 <@t> uic.edu Wed Jan 25 14:36:27 2012 From: amario3 <@t> uic.edu (Andrea Marion) Date: Wed Jan 25 14:36:30 2012 Subject: [Histonet] peroxidase blocking in whole tissues Message-ID: <8ce0b82221221369ad8aa00e22b0d742.squirrel@webmail.uic.edu> Hello histonet, I am working on a whole-mount Pecam immunostain on mouse embryo hearts. During the peroxidase blocking step (1% H2O2 in PBS) there was visible vigorous bubbling in the tissue which caused the heart to swell up. I will try a lower concentration of H2O2 and also prepare the solution in MeOH which I have read is "more gentle" on tissues with high endogenous peroxidase activity. My questions are 1) Why is MeOH "more gentle" than H2O2 in aqueous solutions? and 2) Is there anyone out there in histoland who has done this experiment and can recommend a protocol? Thank you, Andrea Marion Graduate Student University of Illinois at Chicago From Nancy_Schmitt <@t> pa-ucl.com Wed Jan 25 14:40:11 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Jan 25 14:40:15 2012 Subject: [Histonet] overfixation of GI biopsies? Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36B74532@PEITHA.wad.pa-ucl.com> Hi to all- I am looking through documentation and some say there is no such thing as overfixation - some say there is . Does overfixation only pertain to IHC? We are seeing some trouble with GI biopsies on Mon. morn - this is after they have been in formalin for >24 hours - any GI specialists out there? Thanks so much Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From TNMayer <@t> mdanderson.org Wed Jan 25 14:45:12 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 25 14:45:17 2012 Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 32 In-Reply-To: <657c35aa-b30e-42ed-a3b2-db35f0f5b9b7@DCPWPRTR02.mdanderson.edu> References: <657c35aa-b30e-42ed-a3b2-db35f0f5b9b7@DCPWPRTR02.mdanderson.edu> Message-ID: Also, to help cut the tissues, I face at room temperature (very slowly), then I place my uterus/prostate blocks on ice with lots of water, then I cut them last. This helps to allow the blocks to soak up the water and soften some, plus allows nice thin sections. I do agree with Andi that the processing procedure is too long with too many 100% alcohols. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ Message: 16 Date: Wed, 25 Jan 2012 07:22:07 -0800 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] Reg: Tissue is hard to cut after processing Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Arun, It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. Andi Grantham On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: > Dear all, > > We are having a hard time in cutting blocks. All our blocks especially > uterus, prostate chips etc became very hard after processing, we cant even > trim the blocks its very hard. > we use SLEE MTM automated tissue processor > and our schedule is > 1, Formalin - 1hr - ambient - 40 degree > 2, Formalin - 2 hr - vacuum - 40 degree > 3, 70% ethanol - 1hr - vacuum - Room temp > 4, 95% ethanol - 1hr - vacuum - Room temp > 5 and 6- 100% ethanol - 1hr each - vacuum - room temp > 7, 100% ethanol - 1.30 hr - vacuum - room temp > 8, 9 and 10 - Xylene - 1 hr each - vacuum - 30, 35, 40 > degree respectively > 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. > > We use Merck paraffin wax of 56 degree M.P > > Is there any problem with tissue processing schedule or something else is > wrong? > > We are in a big problem really expecting some valuable suggestion and > advices. > > with regards > > > > ARUN JYOTHI S.P. > Histotechnologist > United Laboratories Co. > Kuwait > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > *************** From amosbrooks <@t> gmail.com Wed Jan 25 15:02:30 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jan 25 15:02:36 2012 Subject: [Histonet] neutrophil marker for FFPE mouse tissue Message-ID: Hi, I agree with Liz Chlipala's suggestion for the Serotec antibody. It works nicely. I recommend a protein block though. It does tend toward the background. I use Proteinase K rather than HIER. Amos On Wed, Jan 25, 2012 at 11:22 AM, wrote: > Message: 20 > Date: Wed, 25 Jan 2012 08:11:14 -0800 (PST) > From: Kim Merriam > Subject: [Histonet] neutrophil marker for FFPE mouse tissue > To: Histonet > Message-ID: > <1327507874.8565.YahooMailNeo@web130104.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hello! > > What antibodies are people using to stain for neutrophils in FFPE mouse > tissue? > > Kim > > > > Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA > From patjnm <@t> gwumc.edu Wed Jan 25 15:07:35 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Wed Jan 25 15:07:41 2012 Subject: [Histonet] interview questions Message-ID: <4F2028C6.DB55.001F.1@gwumc.edu> I think it is great that you are using the histonet this way to ask probing questions. SInce they are replacing you, I would get a feel for how they would do your job by telling them the general taskings and letting them tell you what they know about all of the taskings. I have also found that some people can really answer questions well but then when they are asked to perform, there seems to be some, um "confusement". Yes confusement, my own werd. Seriously if I interview anyone again, I will see if I can ask them to mock going through some of the taks, like without an actual blade or sample mock setting up to cut, embed, stain, maybe even some simple computer tasks. ANy chance you can overlap with the replacement? Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8196 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From patjnm <@t> gwumc.edu Wed Jan 25 15:11:51 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Wed Jan 25 15:11:57 2012 Subject: [Histonet] oct to paraffin Message-ID: <4F2029C6.DB55.001F.1@gwumc.edu> If you can spare some nbf the best thing to do would be to place the oct block right into NBF and use that as the "wash" and then move onto a fresh change of NBF. I would avoid straight water. NBF has enough water in it to rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough and a safe bet. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8196 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From andreahooper <@t> rocketmail.com Wed Jan 25 16:26:53 2012 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Wed Jan 25 16:28:02 2012 Subject: [Histonet] Signal amplification In-Reply-To: References: <657c35aa-b30e-42ed-a3b2-db35f0f5b9b7@DCPWPRTR02.mdanderson.edu> Message-ID: <21EBD290-79ED-45B1-B77A-F2FAD62445FF@rocketmail.com> What kits/vendors are people using for their CSA/tyramide amplification protocols? Thanks in advance! Andrea On Jan 25, 2012, at 3:45 PM, "Mayer,Toysha N" wrote: > Also, to help cut the tissues, I face at room temperature (very slowly), then I place my uterus/prostate blocks on ice with lots of water, then I cut them last. This helps to allow the blocks to soak up the water and soften some, plus allows nice thin sections. > > I do agree with Andi that the processing procedure is too long with too many 100% alcohols. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > ------------------------------ > > Message: 16 > Date: Wed, 25 Jan 2012 07:22:07 -0800 > From: "Grantham, Andrea L - (algranth)" > Subject: Re: [Histonet] Reg: Tissue is hard to cut after processing > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Arun, > It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. > Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. > If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. > You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. > > Andi Grantham > > On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: > >> Dear all, >> >> We are having a hard time in cutting blocks. All our blocks especially >> uterus, prostate chips etc became very hard after processing, we cant even >> trim the blocks its very hard. >> we use SLEE MTM automated tissue processor >> and our schedule is >> 1, Formalin - 1hr - ambient - 40 degree >> 2, Formalin - 2 hr - vacuum - 40 degree >> 3, 70% ethanol - 1hr - vacuum - Room temp >> 4, 95% ethanol - 1hr - vacuum - Room temp >> 5 and 6- 100% ethanol - 1hr each - vacuum - room temp >> 7, 100% ethanol - 1.30 hr - vacuum - room temp >> 8, 9 and 10 - Xylene - 1 hr each - vacuum - 30, 35, 40 >> degree respectively >> 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. >> >> We use Merck paraffin wax of 56 degree M.P >> >> Is there any problem with tissue processing schedule or something else is >> wrong? >> >> We are in a big problem really expecting some valuable suggestion and >> advices. >> >> with regards >> >> >> >> ARUN JYOTHI S.P. >> Histotechnologist >> United Laboratories Co. >> Kuwait >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > *************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jan 25 18:14:13 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 25 18:14:23 2012 Subject: [Histonet] Interview Questions In-Reply-To: References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu>, <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net>, <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> Message-ID: <9963E417AAAA4CEA9E9A0713472497C4@JoePC> I used to give a 10 question test on general histology. I also had the expected answers written down and on my copy. Was accused once of being a racist. What saved me was having the answers in front of me. The person didn't get one answer correct. I had a couple of embedding questions, some cutting, special stains, immunos and some QC questions. I gave the interviewee the test while I was reviewing their resume. I would also see what their facial expressions were too. I had one person tell me they didn't do specials or immunos and didn't like embedding either. When I asked if they liked filing blocks and slides, they really would rather have a lab aide do it. This person didn't have to finish the test. Too make matters worse, she wore a denim miniskirt to boot. Just my three cents Joe ----- Original Message ----- From: "joelle weaver" To: ; ; ; "Histonet" Sent: Wednesday, January 25, 2012 12:02 PM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: trathborne@somerset-healthcare.com > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 25 Jan 2012 17:47:01 +0000 > Subject: RE: [Histonet] Interview Questions > CC: > > If your replacement will be doing actual histology, will your institution > permit the applicant to embed and cut? Can you sit down at a multi-head > scope and review slides with them? > What will the person be responsible for? Do they have experience with all > of these tasks? What would they do in a crisis situation (you can make up > one yourself that would be plausible). > People who volunteer in their personal lives, may do the same at work. Ask > how they juggle their schedule though, if there is a lot going on in their > personal lives. Be careful with how you ask these questions though. Your > HR department should be able to give you guidance in how to phrase things. > Good luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, > Bill > Sent: Wednesday, January 25, 2012 12:19 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was able > to answer all the right "histo" questions and so I hired him. He turned > out to be a poser, who, shortly after I fired him showed up at a local > university with a lab coat that listed him as "Dr." He had indeed worked > in a histo lab, but as a lab assistant, and so the the understanding of > what a histologist does was well rehearsed. (BTW, it topok me about two > weeks to catch on, though the more experienced techs in the department > figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them were > particularly useful or telling. For inistance, they would not ask if the > person was an histo tech, but would simply ask, did he indeed work at your > institution? > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done as > well weeding them out by histo questions. I'm sure your HR folks will do > a fine job of this. > > Also, once you have determined that they actually have the skills, or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in the > mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, > Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are > not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you received > this email in error, immediately reply to the sender and delete the > message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.Hoy <@t> UTSouthwestern.edu Wed Jan 25 18:54:45 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Jan 25 18:54:50 2012 Subject: [Histonet] Interview Questions In-Reply-To: <433be01ed39a420dae6a8c2770ccd0b1@SWMSHUB3.swmed.org> Message-ID: A long time ago, I worked in a lab where we had a manual dexterity test that we gave to all applicants for medical technologist or histotechnologist positions. It was designed by psychologists to test hand-eye coordination, spatial orientation, fine motor skills, and (to a certain degree) reasoning skills. Our HR department also signed off on use of the test. We found that doing well on the test did not predict an employee with good skills, but doing poorly on the test pointed out those who would never be able to cope in our laboratory. I'll dig through some old files and see if I can find more info on this test. Two questions I have always asked: 1. Describe the characteristics of the best supervisor/manager for whom you have worked. 2. Describe the characteristics of the worst supervisor/manager for whom you have worked. (For new graduates, I substitute "professor" for "manager.") One applicant, who seemed to have all of the technical skills, described his worst manager as one who sounded just like me: hands-on, involved in day-to-day operations of the lab, picky about being to work on time, perfectionist. I knew right then that we would not be a fit. Good luck with your search! Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 1/25/12 12:09 PM, "Louise Renton" wrote: > Just to be devil's advocate here...... > > asking a person to "prove" their skills - what happens, if through > nervousness, or being unfamiliar with the equipment, they injure > themselves. Where does the liability lie? > > Rather ask questions regarding cutting speed, way in which tissue is > embedded etc, and review the person's skill during an agreed probationary > period. If they are not what u expected, then you can get rid of them..... From koellingr <@t> comcast.net Wed Jan 25 20:59:49 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jan 25 21:00:08 2012 Subject: [Histonet] Interview Questions In-Reply-To: Message-ID: <1983057611.84976.1327546789860.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say "I object"? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? Ray Seattle, WA ----- Original Message ----- From: "joelle weaver" To: trathborne@somerset-healthcare.com, billodonnell@catholichealth.net, sbreeden@nmda.nmsu.edu, "Histonet" Sent: Wednesday, January 25, 2012 10:02:39 AM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: trathborne@somerset-healthcare.com > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu > Date: Wed, 25 Jan 2012 17:47:01 +0000 > Subject: RE: [Histonet] Interview Questions > CC: > > If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? > What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). > People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. > Good luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > Sent: Wednesday, January 25, 2012 12:19 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was able to answer all the right "histo" questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as "Dr." He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? > > The place where I worked required little or nothing for proof of experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. > > Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Jan 25 21:48:43 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jan 25 21:49:00 2012 Subject: [Histonet] Interview Questions In-Reply-To: Message-ID: <786758784.86760.1327549723650.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Not upset in the least. Just posting my own questions and doubts within the parameters of the situation. When the Chinese philosopher who fell asleep under a tree and dreamt he was a butterfly and then spent the rest of his life "asking" if he was a human who fell asleep under a tree and dreamt he was a butterfly or was really a butterfly dreaming he was a human who fell asleep under a tree who........? Wouldn't say he at all took offense to the situation; pondering, reflecting and just asking a question. Ray Seattle Sent from my Bedroom Wireless Laptop ----- Original Message ----- From: "joelle weaver" To: koellingr@comcast.net Sent: Wednesday, January 25, 2012 7:26:37 PM Subject: Re: [Histonet] Interview Questions Well I am sorry that you took such offense, but some jobs do have say words/minute typing for example. I guess the variation in qualified individuals leads me to not be upset to be asked to demonstrate tasks within the assigned duties. I think maybe you have simplifed a bit too. I think all those professions,such as attorneys have to do much more than you indicate_sorry this upset you Sent from my Verizon Wireless BlackBerry -----Original Message----- From: koellingr@comcast.net Date: Thu, 26 Jan 2012 02:59:49 To: Cc: ; ; ; Subject: Re: [Histonet] Interview Questions This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say "I object"? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? Ray Seattle, WA ---------------- From: "joelle weaver" To: trathborne@somerset-healthcare.com, billodonnell@catholichealth.net, sbreeden@nmda.nmsu.edu, "Histonet" Sent: Wednesday, January 25, 2012 10:02:39 AM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: trathborne@somerset-healthcare.com > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu > Date: Wed, 25 Jan 2012 17:47:01 +0000 > Subject: RE: [Histonet] Interview Questions > CC: > > If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? > What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). > People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. > Good luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > Sent: Wednesday, January 25, 2012 12:19 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was able to answer all the right "histo" questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as "Dr." He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? > > The place where I worked required little or nothing for proof of experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. > > Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Thu Jan 26 01:08:07 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Thu Jan 26 01:08:14 2012 Subject: [Histonet] Interview Questions In-Reply-To: <786758784.86760.1327549723650.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <786758784.86760.1327549723650.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: My most hated question in interviews is "where do you see yourself in 5 years?"....answer - in your job asking stupid questions! On Thu, Jan 26, 2012 at 5:48 AM, wrote: > Not upset in the least. Just posting my own questions and doubts within > the parameters of the situation. When the Chinese philosopher who fell > asleep under a tree and dreamt he was a butterfly and then spent the rest > of his life "asking" if he was a human who fell asleep under a tree and > dreamt he was a butterfly or was really a butterfly dreaming he was a human > who fell asleep under a tree who........? Wouldn't say he at all took > offense to the situation; pondering, reflecting and just asking a question. > > > Ray > Seattle > Sent from my Bedroom Wireless Laptop > > ----- Original Message ----- > From: "joelle weaver" > To: koellingr@comcast.net > Sent: Wednesday, January 25, 2012 7:26:37 PM > Subject: Re: [Histonet] Interview Questions > > Well I am sorry that you took such offense, but some jobs do have say > words/minute typing for example. I guess the variation in qualified > individuals leads me to not be upset to be asked to demonstrate tasks > within the assigned duties. I think maybe you have simplifed a bit too. I > think all those professions,such as attorneys have to do much more than you > indicate_sorry this upset you > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: koellingr@comcast.net > Date: Thu, 26 Jan 2012 02:59:49 > To: > Cc: ; ; > ; > Subject: Re: [Histonet] Interview Questions > > This is certainly an interesting thread and I generally hate to get into > these ever but I still can't figure out one thing and never have over all > these years in pathology. What other endeavor in life and job seeking is an > on-the-spot demo that you can do something required at a job interview? > Does a lawyer have to go into a courtroom for 5 minutes and show he/she can > say "I object"? Does a sanitation worker have to go round the block once > and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show > he/she can use a stethoscope? Does a bricklayer have to show he/she can lay > 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show > they can stain 6 tubes with CD4 and CD 8 and successfully put them on a > flow cytometer? Does an actuary have to show they can really add 100 > 4-digit numbers on a calculator without a mistake? Does a grocery bagger > boy /girl have to show they can put x number of items in 3 bags? Does a > Pathologist have to show they know how to turn on a microscope and look > through it? Does a peanut counter have to show they can count peanuts? I > just can't get into my mind the necessity of someone having to cut to show > they can cut? What other profession does this at an interview? Now > certainly you can come up with scenarios where it might be important to > find out. A brand new histotech whose only cut 3 blocks in their life. A > tech from the deepest, darkest nether regions of the earth where you cannot > check on their background. But a tech whose has been working cutting the > last 3 or 7 or 15 years and you've verified with a previous company that is > exactly what they did; how will them cutting for 10 minutes further > stratify them into yes or no categories. If 2 potential techs cut and one > finishes in 9 minutes and one in 10 minutes, is that a true qualifier or > disqualifier of what they can do cutting? There are a myriad of things I'd > love to know and always ask; personality, job knowledge, wants, desires, > needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give > blood because I HATE anyone sticking a needle in me. But I have a really > needed blood type. Should nervousness each time disqualify me. This still > boggles my mind about what is being accomplished with cutting during an > interview? > > > Ray > Seattle, WA > > > ---------------- > From: "joelle weaver" > To: trathborne@somerset-healthcare.com, billodonnell@catholichealth.net, > sbreeden@nmda.nmsu.edu, "Histonet" > Sent: Wednesday, January 25, 2012 10:02:39 AM > Subject: RE: [Histonet] Interview Questions > > > Love this! I always want to do demonstration during technical interviews, > but usually get "shot down" from managers and argued with in general, as in > people don't feel that they should have to "prove" they can do histology. > This perception, I never got, because I always saw it as in a job > interview-in what other situation are you more trying to "prove" or impress > with your knowledge, attitude, skills and experience? If you do bench work, > you can tell in just a few minutes of observation much more information > than you could get with quite a few questions. To be fair, I take into > account nervousness, being closely observed, and lack of familiarity with > equipment etc. I don't know, I think its fair if those are important skills > to the position/role. Was not sure if Sara's job was mostly technical > though, so thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathborne@somerset-healthcare.com > > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > > Date: Wed, 25 Jan 2012 17:47:01 +0000 > > Subject: RE: [Histonet] Interview Questions > > CC: > > > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at a > multi-head scope and review slides with them? > > What will the person be responsible for? Do they have experience with > all of these tasks? What would they do in a crisis situation (you can make > up one yourself that would be plausible). > > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on in > their personal lives. Be careful with how you ask these questions though. > Your HR department should be able to give you guidance in how to phrase > things. > > Good luck. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > > Sent: Wednesday, January 25, 2012 12:19 PM > > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Interview Questions > > > > It would seem that questions like "How do you feel about cannibalism?" > > might also be out but might be far more helpful; than "phone" questions. > > > > > > On the serious side, when I was much younger I hired a person who was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at a > local university with a lab coat that listed him as "Dr." He had indeed > worked in a histo lab, but as a lab assistant, and so the the understanding > of what a histologist does was well rehearsed. (BTW, it topok me about two > weeks to catch on, though the more experienced techs in the department > figured it out almost right away) > > > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them were > particularly useful or telling. For inistance, they would not ask if the > person was an histo tech, but would simply ask, did he indeed work at your > institution? > > > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > > > Today, however, reference checking is a lot easier and more reliable. > > > > I guess my point here is that a good reference check needs to be done as > well weeding them out by histo questions. I'm sure your HR folks will do a > fine job of this. > > > > Also, once you have determined that they actually have the skills, or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in the > mind of the applicant. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > > Sent: Wednesday, January 25, 2012 10:52 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Interview Questions > > > > So far, I am TOTALLY impressed and so grateful for your suggestions. > > And here's why... did I ever tell anyone out there what the FIRST > > question I was asked by the pathologist at my interview? It was..... > > (wait for it....) > > > > > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > > I sure don't want someone to remember ME that way!!! > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical > Center > > and are intended only for the addressee. The information contained in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From lpwenk <@t> sbcglobal.net Thu Jan 26 04:15:29 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jan 26 04:20:19 2012 Subject: [Histonet] Interview Questions In-Reply-To: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062D38@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <247CA5BC24A543D6B4790A8F5C18191C@HP2010> Ask the type of questions that require multiple layers, with explanations and other examples. Give me a time when XXXX, and how you handled it? Why? What else do you think you could have done? Why? What was the outcome? Were you satisfied with it? Why? Can you give me another example? Ask at least three "deeper" questions on each topic. Most people have a "prepared" answer. You want to get to the "have to really think about it" level, where you might find out what they really think. Don't accept a one sentence answer. Give long pauses. People get uncomfortable, and just start talking to fill in the silence. Again, that's when you might find out what they really think. Talk about when things didn't go right. Find out how they handle those times. Most people can go with the flow when everything is going smoothly at work. It's those "other" times that we need to know how people will react. Don't accept "oh, that's never happened to me". EVERYONE has had a negative time or person at work. If they won't talk about it, then they won't deal with it when it happens at the new job, or they will handle in a way that your business and coworkers won't like. Ask about: - negative times or people - who they didn't like working with or had conflict with, or a time things didn't go right at work, and why and what they did to help the situation, and what was the outcome, looking back what they could do differently. - time they needed to be flexible (same type of follow up questions) - time they had or work as a team, or a time when a team they were working on didn't work well together - time they had to change procedures or the work flow or priorities at work. - why are they deciding to change their job at this time. Why, why, why. - what they do in the slow times at work - the best manager they ever had, the worst. why, why, why - how they manage doing several tasks at the same time, how they keep track of the projects - stressful time at work - why was it stressful, what did they do to handle it, what would they do different. - continuing education - how they keep themselves informed about changes in the field - time they were evaluated unfairly, how they handled it, what was outcome, how they could have handled it differently. - significant accomplishment at work - what they did, why, - what their plans are if not accepted into that position (find out if they are willing to do anything to increase their chances next time (attend workshops, online CEU, go back to college, become ASCP certified, whatever)) Last, if the job requires that they be ASCP certified (and I hope it does), get a copy of the certificate, and then contact ASCP with their name and ASCP certification number. Get it verified from ASCP that the number matches the name. People are copying someone else's certificate, whiting out the name, printing over their own name, copying it again, and passing it off as their own. Peggy Wenk -----Original Message----- From: Breeden, Sara Sent: Wednesday, January 25, 2012 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this "fortunate" before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with "meat" to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMP <@t> stowers.org Thu Jan 26 05:35:30 2012 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Thu Jan 26 05:35:40 2012 Subject: [Histonet] Interview Questions In-Reply-To: References: <786758784.86760.1327549723650.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9966DE109@EXCHMB-02.stowers-institute.org> I wish there was a "like" icon to click for this response! LOL!! Seriously though, I know there is a need for the questions and I believe it doesn't hurt for a person to ask to 'show their skills'. Histology is a specialized field and it takes 'special' people to get the job done. Just my 2 cents. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Thursday, January 26, 2012 1:08 AM To: Histonet Subject: Re: [Histonet] Interview Questions My most hated question in interviews is "where do you see yourself in 5 years?"....answer - in your job asking stupid questions! On Thu, Jan 26, 2012 at 5:48 AM, wrote: > Not upset in the least. Just posting my own questions and doubts > within the parameters of the situation. When the Chinese philosopher > who fell asleep under a tree and dreamt he was a butterfly and then > spent the rest of his life "asking" if he was a human who fell asleep > under a tree and dreamt he was a butterfly or was really a butterfly > dreaming he was a human who fell asleep under a tree who........? > Wouldn't say he at all took offense to the situation; pondering, reflecting and just asking a question. > > > Ray > Seattle > Sent from my Bedroom Wireless Laptop > > ----- Original Message ----- > From: "joelle weaver" > To: koellingr@comcast.net > Sent: Wednesday, January 25, 2012 7:26:37 PM > Subject: Re: [Histonet] Interview Questions > > Well I am sorry that you took such offense, but some jobs do have say > words/minute typing for example. I guess the variation in qualified > individuals leads me to not be upset to be asked to demonstrate tasks > within the assigned duties. I think maybe you have simplifed a bit > too. I think all those professions,such as attorneys have to do much > more than you indicate_sorry this upset you Sent from my Verizon > Wireless BlackBerry > > -----Original Message----- > From: koellingr@comcast.net > Date: Thu, 26 Jan 2012 02:59:49 > To: > Cc: ; > ; > ; > Subject: Re: [Histonet] Interview Questions > > This is certainly an interesting thread and I generally hate to get > into these ever but I still can't figure out one thing and never have > over all these years in pathology. What other endeavor in life and job > seeking is an on-the-spot demo that you can do something required at a job interview? > Does a lawyer have to go into a courtroom for 5 minutes and show > he/she can say "I object"? Does a sanitation worker have to go round > the block once and show he/she can empty 9 cans in 5 minutes? Does a > doctor need to show he/she can use a stethoscope? Does a bricklayer > have to show he/she can lay 20 bricks in 2 minutes? Or fail the > interview? Does a med tech have to show they can stain 6 tubes with > CD4 and CD 8 and successfully put them on a flow cytometer? Does an > actuary have to show they can really add 100 4-digit numbers on a > calculator without a mistake? Does a grocery bagger boy /girl have to > show they can put x number of items in 3 bags? Does a Pathologist have > to show they know how to turn on a microscope and look through it? > Does a peanut counter have to show they can count peanuts? I just > can't get into my mind the necessity of someone having to cut to show > they can cut? What other profession does this at an interview? Now > certainly you can come up with scenarios where it might be important > to find out. A brand new histotech whose only cut 3 blocks in their > life. A tech from the deepest, darkest nether regions of the earth > where you cannot check on their background. But a tech whose has been > working cutting the last 3 or 7 or 15 years and you've verified with a > previous company that is exactly what they did; how will them cutting > for 10 minutes further stratify them into yes or no categories. If 2 > potential techs cut and one finishes in 9 minutes and one in 10 > minutes, is that a true qualifier or disqualifier of what they can do > cutting? There are a myriad of things I'd love to know and always ask; > personality, job knowledge, wants, desires, needs, ambitions, etc, > etc, etc. My blood pressure skyrockets when I give blood because I > HATE anyone sticking a needle in me. But I have a really needed blood > type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? > > > Ray > Seattle, WA > > > ---------------- > From: "joelle weaver" > To: trathborne@somerset-healthcare.com, > billodonnell@catholichealth.net, sbreeden@nmda.nmsu.edu, "Histonet" > > Sent: Wednesday, January 25, 2012 10:02:39 AM > Subject: RE: [Histonet] Interview Questions > > > Love this! I always want to do demonstration during technical > interviews, but usually get "shot down" from managers and argued with > in general, as in people don't feel that they should have to "prove" they can do histology. > This perception, I never got, because I always saw it as in a job > interview-in what other situation are you more trying to "prove" or > impress with your knowledge, attitude, skills and experience? If you > do bench work, you can tell in just a few minutes of observation much > more information than you could get with quite a few questions. To be > fair, I take into account nervousness, being closely observed, and > lack of familiarity with equipment etc. I don't know, I think its fair > if those are important skills to the position/role. Was not sure if > Sara's job was mostly technical though, so thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathborne@somerset-healthcare.com > > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > > Date: Wed, 25 Jan 2012 17:47:01 +0000 > > Subject: RE: [Histonet] Interview Questions > > CC: > > > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at > a multi-head scope and review slides with them? > > What will the person be responsible for? Do they have experience > > with > all of these tasks? What would they do in a crisis situation (you can > make up one yourself that would be plausible). > > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on > in their personal lives. Be careful with how you ask these questions though. > Your HR department should be able to give you guidance in how to > phrase things. > > Good luck. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, > Bill > > Sent: Wednesday, January 25, 2012 12:19 PM > > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Interview Questions > > > > It would seem that questions like "How do you feel about cannibalism?" > > might also be out but might be far more helpful; than "phone" questions. > > > > > > On the serious side, when I was much younger I hired a person who > > was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at > a local university with a lab coat that listed him as "Dr." He had > indeed worked in a histo lab, but as a lab assistant, and so the the > understanding of what a histologist does was well rehearsed. (BTW, it > topok me about two weeks to catch on, though the more experienced > techs in the department figured it out almost right away) > > > > To be fair, it was during a time in hiring history when HR > > departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them > were particularly useful or telling. For inistance, they would not ask > if the person was an histo tech, but would simply ask, did he indeed > work at your institution? > > > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > > > Today, however, reference checking is a lot easier and more reliable. > > > > I guess my point here is that a good reference check needs to be > > done as > well weeding them out by histo questions. I'm sure your HR folks will > do a fine job of this. > > > > Also, once you have determined that they actually have the skills, > > or a > realistic potential of gaining them, questions concerning dynamics of > interaction are appropriate, though may lead to wrong impressions in > the mind of the applicant. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > > Sent: Wednesday, January 25, 2012 10:52 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Interview Questions > > > > So far, I am TOTALLY impressed and so grateful for your suggestions. > > And here's why... did I ever tell anyone out there what the FIRST > > question I was asked by the pathologist at my interview? It was..... > > (wait for it....) > > > > > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > > I sure don't want someone to remember ME that way!!! > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely > > for > the named addressee(s) and contain confidential information. If you > are not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical > Center > > and are intended only for the addressee. The information contained > > in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection > > and/or exemption from disclosure under applicable law. Unauthorized > > forwarding, printing, copying, distribution, or use of such > > information is strictly prohibited and may be unlawful. If you are > > not the addressee, please promptly delete this message and notify > > the sender of the delivery error by e-mail or you may call Somerset > > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, event > > listings, health information and more. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Thu Jan 26 07:18:26 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jan 26 07:18:34 2012 Subject: [Histonet] Signal amplification In-Reply-To: <21EBD290-79ED-45B1-B77A-F2FAD62445FF@rocketmail.com> References: <657c35aa-b30e-42ed-a3b2-db35f0f5b9b7@DCPWPRTR02.mdanderson.edu> <21EBD290-79ED-45B1-B77A-F2FAD62445FF@rocketmail.com> Message-ID: <1327583906.13977.YahooMailNeo@web130103.mail.mud.yahoo.com> We use Perkin Elmer for biotinyl tyramide and invitrogen for fluroescent tyramide amp (they sell several kits with differnt flourochromes, including streptavidin TSA). Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Andrea Hooper To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 25, 2012 5:26 PM Subject: [Histonet] Signal amplification What kits/vendors are people using for their CSA/tyramide amplification protocols? Thanks in advance! Andrea On Jan 25, 2012, at 3:45 PM, "Mayer,Toysha N" wrote: > Also, to help cut the tissues, I face at room temperature (very slowly), then I place my uterus/prostate blocks on ice with lots of water, then I cut them last. This helps to allow the blocks to soak up the water and soften some, plus allows nice thin sections. > > I do agree with Andi that the processing procedure is too long with too many 100% alcohols. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > ------------------------------ > > Message: 16 > Date: Wed, 25 Jan 2012 07:22:07 -0800 > From: "Grantham, Andrea L - (algranth)" > Subject: Re: [Histonet] Reg: Tissue is hard to cut after processing > Cc: "histonet@lists.utsouthwestern.edu" >? ? > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Arun, > It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. > Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. > If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. > You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. > > Andi Grantham > > On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: > >> Dear all, >> >> We are having a hard time in cutting blocks. All our blocks especially >> uterus, prostate chips etc became very hard after processing, we cant even >> trim the blocks its very hard. >> we use SLEE MTM automated tissue processor >> and our schedule is >> 1, Formalin? ? ? ? ? ? ? ? ? ? ? ? ? ? - 1hr? ? ? ? ? - ambient? - 40 degree >> 2, Formalin? ? ? ? ? ? ? ? ? ? ? ? ? ? - 2 hr? ? ? ? ? - vacuum - 40 degree >> 3, 70% ethanol? ? ? ? ? ? ? ? ? ? ? - 1hr? ? ? ? ? - vacuum? - Room temp >> 4, 95% ethanol? ? ? ? ? ? ? ? ? ? ? - 1hr? ? ? ? ? - vacuum? - Room temp >> 5 and 6- 100% ethanol? ? ? ? ? ? - 1hr each? - vacuum - room temp >> 7, 100% ethanol? ? ? ? ? ? ? ? ? ? - 1.30 hr? ? ? - vacuum? -? room temp >> 8, 9 and 10 - Xylene? ? ? ? ? ? ? ? - 1 hr each? - vacuum? - 30, 35, 40 >> degree respectively >> 11,12,13 and 14 - paraffin wax? - 1 hr each? - vacuum -? 63 degree for all. >> >> We use Merck paraffin wax of 56 degree M.P >> >> Is there any problem with tissue processing schedule or something else is >> wrong? >> >> We are in a big problem really expecting some valuable suggestion and >> advices. >> >> with regards >> >> >> >> ARUN JYOTHI S.P. >> Histotechnologist >> United Laboratories Co. >> Kuwait >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > *************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy.crumpton <@t> tuality.org Thu Jan 26 08:33:24 2012 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Thu Jan 26 08:33:30 2012 Subject: [Histonet] RE: Interview Questions Message-ID: The last two times we interviewed a person we gave them a written skills test before they sat down for the face to face interview. It had simple questions on it, like how to mix a percent solution, clean a formalin spill, which chemical was more hazardous than another, how to handle certain types of tissues, how to work computer applications, etc. The test has 15 questions and has a section that includes them writing on a cassette and following written instructions on how to prep a non-gyn cytology. This gave us a lot of skills information about the person and helped us weed out who was qualified. You could tell quite a bit about their knowledge just from the written portion and people seemed less nervous when there wasn't a person hovering over them. Our hospital sees this skills test as the equivalent of a typing test for transcriptionists. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From andreahooper <@t> rocketmail.com Thu Jan 26 08:45:08 2012 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Thu Jan 26 08:45:18 2012 Subject: [Histonet] TMA & tissue sources In-Reply-To: <02DE68BE-CE01-475A-B92A-8EF5AC350026@uct.ac.za> References: <02DE68BE-CE01-475A-B92A-8EF5AC350026@uct.ac.za> Message-ID: > (1) Do those of you who use cyno tissue have a favorite source for purchasing FFPE and frozen blocks? (2) What is evetyone's favorite vendor for frozen TMAs? Thanks, Andrea From relia1 <@t> earthlink.net Thu Jan 26 10:07:38 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 26 10:08:02 2012 Subject: [Histonet] RELIA Solutions EXCLUSIVE !!! Histotech/Supervisor Brand New Lab in Kissimmee, FL Message-ID: <0F4F196DEF0D4C138905770928CCCD9D@ownerf1abaad51> Hi Histonetters!! Boy oh Boy the phones are RINGING off the hook here at RELIA!!! I hope you are having a great day! I am excited to tell you about a brand new position. This opportunity is with a private GI lab in Kissimmee, FL. My client offers excellent pay and benefits. They are looking for someone who is Florida licensed and has at least 3 years of solid routine histology experience grossing is a plus. This lab can work with flexible hours. The schedule is M-F. You would be the sole histotech in the lab. If you are interested in full or part time work days or evenings you should contact me about this position. This is an exclusive opportunity with RELIA Solutions. This client is ready to move quickly. They want to find the right person right away!! If you or someone you know is interested please contact me toll free at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Valerie.Hannen <@t> parrishmed.com Thu Jan 26 10:07:57 2012 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Jan 26 10:08:05 2012 Subject: FW: [Histonet] RE: NCCI policy update--requested link Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> Sorry for my redundancy in sending this message....I could not seem to get my act together in sending it in properly. : ) Valerie -----Original Message----- From: Hannen, Valerie Sent: Wednesday, January 25, 2012 9:37 AM To: 'histonet@list.utsouthwestern.edu' Subject: RE: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 24, 2012 12:05 PM To: 'Lester Raff MD' Subject: RE: [Histonet] RE: NCCI policy update--requested link Ok folks...sorry for revisting this issue...but I must. We are contemplating revamping our immuno stains, we have gotten a proposal from a new vendor, and I have been put to the task of figuring out if this proposal is going to be feasible. I understand that we are only allow to bill per specimen "part" not per block for the immunos, I am a little cloudy on one issue, however. Are we allowed to bill for multiple antibodies on the same specimen part or not? Does this guideline mean that the hospital will only get paid for 1 immuno antibody technical charge even if we do multiple antibodies on that one specimen? Thanks for any and all imput!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Lester Raff MD Sent: Sat 12/31/2011 4:10 PM To: joelle weaver ; Rathborne Toni ; JEllin@yumaregional.org ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu ; Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail is >strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From LRaff <@t> uropartners.com Thu Jan 26 10:11:33 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Jan 26 10:11:41 2012 Subject: [Histonet] RE: NCCI policy update--requested link In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> Message-ID: CAP has promised me they will provide us with guidance on this issue,,,I am still waiting. I am also having a phone conference with our PIN4 triple antibody vendor (Biocare) tomorrow. I will post any interesting follow up. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, January 26, 2012 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link Sorry for my redundancy in sending this message....I could not seem to get my act together in sending it in properly. : ) Valerie -----Original Message----- From: Hannen, Valerie Sent: Wednesday, January 25, 2012 9:37 AM To: 'histonet@list.utsouthwestern.edu' Subject: RE: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 24, 2012 12:05 PM To: 'Lester Raff MD' Subject: RE: [Histonet] RE: NCCI policy update--requested link Ok folks...sorry for revisting this issue...but I must. We are contemplating revamping our immuno stains, we have gotten a proposal from a new vendor, and I have been put to the task of figuring out if this proposal is going to be feasible. I understand that we are only allow to bill per specimen "part" not per block for the immunos, I am a little cloudy on one issue, however. Are we allowed to bill for multiple antibodies on the same specimen part or not? Does this guideline mean that the hospital will only get paid for 1 immuno antibody technical charge even if we do multiple antibodies on that one specimen? Thanks for any and all imput!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Lester Raff MD Sent: Sat 12/31/2011 4:10 PM To: joelle weaver ; Rathborne Toni ; JEllin@yumaregional.org ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu ; Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail is >strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Jan 26 10:16:08 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 26 10:16:19 2012 Subject: [Histonet] RE: NCCI policy update--requested link In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E79B@CHEXCMS10.one.ads.che.org> This is how I understand it. You can bill for multiple different antibodies on the same specimen, but not for multiple blocks of the same ab on the same specimen. e.g. Sentinel nodes on melanoma case... Blocks, A1 A2, A3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Blocks B1, B2, B3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Blocks C1, C2, C3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, January 26, 2012 11:08 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link Sorry for my redundancy in sending this message....I could not seem to get my act together in sending it in properly. : ) Valerie -----Original Message----- From: Hannen, Valerie Sent: Wednesday, January 25, 2012 9:37 AM To: 'histonet@list.utsouthwestern.edu' Subject: RE: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 24, 2012 12:05 PM To: 'Lester Raff MD' Subject: RE: [Histonet] RE: NCCI policy update--requested link Ok folks...sorry for revisting this issue...but I must. We are contemplating revamping our immuno stains, we have gotten a proposal from a new vendor, and I have been put to the task of figuring out if this proposal is going to be feasible. I understand that we are only allow to bill per specimen "part" not per block for the immunos, I am a little cloudy on one issue, however. Are we allowed to bill for multiple antibodies on the same specimen part or not? Does this guideline mean that the hospital will only get paid for 1 immuno antibody technical charge even if we do multiple antibodies on that one specimen? Thanks for any and all imput!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Lester Raff MD Sent: Sat 12/31/2011 4:10 PM To: joelle weaver ; Rathborne Toni ; JEllin@yumaregional.org ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu ; Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail is >strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From chewy71874 <@t> aol.com Thu Jan 26 11:28:03 2012 From: chewy71874 <@t> aol.com (chewy71874@aol.com) Date: Thu Jan 26 11:28:07 2012 Subject: [Histonet] (no subject) Message-ID: <8CEAA66FE0A9517-2F7C-613AA@webmail-d151.sysops.aol.com> http://refrescaweb.com/botiga/images.php?ready45.png From trathborne <@t> somerset-healthcare.com Thu Jan 26 11:55:26 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jan 26 11:55:43 2012 Subject: [Histonet] RE: NCCI policy update--requested link In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E79B@CHEXCMS10.one.ads.che.org> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E79B@CHEXCMS10.one.ads.che.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7F698@SMCMAIL01.somerset-healthcare.com> Also, is the billing (or lack of) the same if you stain with a cocktail as opposed to a double stain? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, January 26, 2012 11:16 AM To: Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: NCCI policy update--requested link This is how I understand it. You can bill for multiple different antibodies on the same specimen, but not for multiple blocks of the same ab on the same specimen. e.g. Sentinel nodes on melanoma case... Blocks, A1 A2, A3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Blocks B1, B2, B3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Blocks C1, C2, C3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, January 26, 2012 11:08 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link Sorry for my redundancy in sending this message....I could not seem to get my act together in sending it in properly. : ) Valerie -----Original Message----- From: Hannen, Valerie Sent: Wednesday, January 25, 2012 9:37 AM To: 'histonet@list.utsouthwestern.edu' Subject: RE: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 24, 2012 12:05 PM To: 'Lester Raff MD' Subject: RE: [Histonet] RE: NCCI policy update--requested link Ok folks...sorry for revisting this issue...but I must. We are contemplating revamping our immuno stains, we have gotten a proposal from a new vendor, and I have been put to the task of figuring out if this proposal is going to be feasible. I understand that we are only allow to bill per specimen "part" not per block for the immunos, I am a little cloudy on one issue, however. Are we allowed to bill for multiple antibodies on the same specimen part or not? Does this guideline mean that the hospital will only get paid for 1 immuno antibody technical charge even if we do multiple antibodies on that one specimen? Thanks for any and all imput!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Lester Raff MD Sent: Sat 12/31/2011 4:10 PM To: joelle weaver ; Rathborne Toni ; JEllin@yumaregional.org ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu ; Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail is >strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JWeems <@t> sjha.org Thu Jan 26 11:58:21 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 26 11:58:30 2012 Subject: [Histonet] RE: NCCI policy update--requested link In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7F698@SMCMAIL01.somerset-healthcare.com> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40D9F@isexstore03> <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E79B@CHEXCMS10.one.ads.che.org> <3AD061FE740D464FAC7BF6B5CFB7570711F7F698@SMCMAIL01.somerset-healthcare.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E7FE@CHEXCMS10.one.ads.che.org> Yes, That is my understanding. I hope Dr. Raff gets some results! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, January 26, 2012 12:55 To: Weems, Joyce; Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: NCCI policy update--requested link Also, is the billing (or lack of) the same if you stain with a cocktail as opposed to a double stain? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, January 26, 2012 11:16 AM To: Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: NCCI policy update--requested link This is how I understand it. You can bill for multiple different antibodies on the same specimen, but not for multiple blocks of the same ab on the same specimen. e.g. Sentinel nodes on melanoma case... Blocks, A1 A2, A3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Blocks B1, B2, B3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Blocks C1, C2, C3 - Melan A, HMB45, S100 - 3 88342s (intead of the 9 that we could have charged last year) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, January 26, 2012 11:08 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link Sorry for my redundancy in sending this message....I could not seem to get my act together in sending it in properly. : ) Valerie -----Original Message----- From: Hannen, Valerie Sent: Wednesday, January 25, 2012 9:37 AM To: 'histonet@list.utsouthwestern.edu' Subject: RE: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 24, 2012 12:05 PM To: 'Lester Raff MD' Subject: RE: [Histonet] RE: NCCI policy update--requested link Ok folks...sorry for revisting this issue...but I must. We are contemplating revamping our immuno stains, we have gotten a proposal from a new vendor, and I have been put to the task of figuring out if this proposal is going to be feasible. I understand that we are only allow to bill per specimen "part" not per block for the immunos, I am a little cloudy on one issue, however. Are we allowed to bill for multiple antibodies on the same specimen part or not? Does this guideline mean that the hospital will only get paid for 1 immuno antibody technical charge even if we do multiple antibodies on that one specimen? Thanks for any and all imput!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] RE: NCCI policy update--requested link -----Original Message----- From: Lester Raff MD Sent: Sat 12/31/2011 4:10 PM To: joelle weaver ; Rathborne Toni ; JEllin@yumaregional.org ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu ; Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue. I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax: 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow. What made them change their minds? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there. May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail is >strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jengirl1014 <@t> yahoo.com Thu Jan 26 12:13:16 2012 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Thu Jan 26 12:13:22 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 Message-ID: <1327601596.45466.YahooMailNeo@web125402.mail.ne1.yahoo.com> Hi Histonetters! ? I have a question that I'm hoping you guys can help me with.? I have a person who brings me mouse intestinal tract with fecal matter still in it.? She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter.? Does anyone have any suggestions as to how best to handle this??? It seems to be getting worse the further into the study she goes. ? Thank you in advance for any help that you give!! It is truly appreciated! ? Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? 410-614-0131 fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu From dreynold <@t> mdanderson.org Thu Jan 26 12:17:53 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Thu Jan 26 12:17:57 2012 Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 35 In-Reply-To: <33d0beb8-9997-4432-94fa-8023ba8bda01@DCPWPRTR01.mdanderson.edu> References: <33d0beb8-9997-4432-94fa-8023ba8bda01@DCPWPRTR01.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F023049632196@DCPWVMBXC0VS3.mdanderson.edu> I can definitely see some of the pitfallls to having someone cut. But it would certainly weed out anyone who didn't even know how to put a block in the microtome. This is also what probation periods are for to see if this person fits with your lab. But have you ever heard of a secretary hired without a typing test or 9 key if that was a heavy part of their job. Even with computers today this is still done. Donna Houston, TX Message: 3 Date: Thu, 26 Jan 2012 02:59:49 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] Interview Questions To: joelle weaver Cc: Histonet Message-ID: <1983057611.84976.1327546789860.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say "I object"? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? Ray Seattle, WA From talulahgosh <@t> gmail.com Thu Jan 26 13:24:46 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jan 26 13:24:49 2012 Subject: Fwd: [Histonet] oct to paraffin In-Reply-To: References: <4F2029C6.DB55.001F.1@gwumc.edu> Message-ID: I may have missed this but does one fix the embryo again in Carnoy's or whatever after it has been washed (assuming it was fixed in the first place) I would think the initial para fixation would be enough and (again after washing in aqueous solution) dehydrating it up to xylene/toluene/non-aqeous solution before the paraffin steps. If one did fix again, would it be washed in 50% EtOH, 70% EtOH, before the fix? Or is this what the NBF washes are for? I have never used NBF, except when I tried to make Serra's with it, and yeah, that's not a good idea. Always use formalin, not NBF in Serra's or you'll get a lovely milky solution that's not fit for fixing. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Wed, Jan 25, 2012 at 4:11 PM, Joseph Madary wrote: > If you can spare some nbf the best thing to do would be to place the oct > block right into NBF and use that as the "wash" and then move onto a fresh > change of NBF. I would avoid straight water. NBF has enough water in it to > rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough > and a safe bet. > > Nick Madary, HT/HTL(ASCP)QIHC > George Washington University > Pathology Core Laboratory > Ross Hall, Room 706 > 23rd and I Street NW > Washington D.C. 20037 > 202.994.8196 > patjnm@gwumc.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ttruscot <@t> vetmed.wsu.edu Thu Jan 26 15:31:01 2012 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jan 26 15:39:57 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 In-Reply-To: <1327601596.45466.YahooMailNeo@web125402.mail.ne1.yahoo.com> References: <1327601596.45466.YahooMailNeo@web125402.mail.ne1.yahoo.com> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A002718833678@CVM76.vetmed.wsu.edu> Hi Jennifer, I am by no means an expert on this, but have done a few mouse guts. I think that trying to flush out the feces with formalin or saline soon after necropsy, would help preserve the mucosa. The bacteria in the gut start breaking down the mucosa soon after death. Perhaps the gut is thin enough to fix rapidly enough to prevent damage to the mucosa without flushing. If that is the case, then flushing out the feces after fixation might help the quality of your slides. You may have to get permission to open up the gut to flush out the feces. It may hinge on how the tissue needs to be trimmed and oriented. Good luck, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes Sent: Thursday, January 26, 2012 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 Hi Histonetters! ? I have a question that I'm hoping you guys can help me with.? I have a person who brings me mouse intestinal tract with fecal matter still in it.? She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter.? Does anyone have any suggestions as to how best to handle this??? It seems to be getting worse the further into the study she goes. ? Thank you in advance for any help that you give!! It is truly appreciated! ? Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? 410-614-0131 fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Jan 26 15:39:59 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 26 15:40:26 2012 Subject: [Histonet] Interview Questions In-Reply-To: <9963E417AAAA4CEA9E9A0713472497C4@JoePC> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu>, <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net>, <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> <9963E417AAAA4CEA9E9A0713472497C4@JoePC> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16CE9@xmdb02.nch.kids> Joe, I would never wear a denim miniskirt! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, 26 January 2012 11:14 AM To: joelle weaver; trathborne@somerset-healthcare.com; billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; Histonet Subject: Re: [Histonet] Interview Questions I used to give a 10 question test on general histology. I also had the expected answers written down and on my copy. Was accused once of being a racist. What saved me was having the answers in front of me. The person didn't get one answer correct. I had a couple of embedding questions, some cutting, special stains, immunos and some QC questions. I gave the interviewee the test while I was reviewing their resume. I would also see what their facial expressions were too. I had one person tell me they didn't do specials or immunos and didn't like embedding either. When I asked if they liked filing blocks and slides, they really would rather have a lab aide do it. This person didn't have to finish the test. Too make matters worse, she wore a denim miniskirt to boot. Just my three cents Joe ----- Original Message ----- From: "joelle weaver" To: ; ; ; "Histonet" Sent: Wednesday, January 25, 2012 12:02 PM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: trathborne@somerset-healthcare.com > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 25 Jan 2012 17:47:01 +0000 > Subject: RE: [Histonet] Interview Questions > CC: > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at > a multi-head scope and review slides with them? > What will the person be responsible for? Do they have experience with > all of these tasks? What would they do in a crisis situation (you can > make up one yourself that would be plausible). > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on > in their personal lives. Be careful with how you ask these questions > though. Your HR department should be able to give you guidance in how to phrase things. > Good luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > O'Donnell, Bill > Sent: Wednesday, January 25, 2012 12:19 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at > a local university with a lab coat that listed him as "Dr." He had > indeed worked in a histo lab, but as a lab assistant, and so the the > understanding of what a histologist does was well rehearsed. (BTW, it > topok me about two weeks to catch on, though the more experienced > techs in the department figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them > were particularly useful or telling. For inistance, they would not ask > if the person was an histo tech, but would simply ask, did he indeed > work at your institution? > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done > as well weeding them out by histo questions. I'm sure your HR folks > will do a fine job of this. > > Also, once you have determined that they actually have the skills, or > a realistic potential of gaining them, questions concerning dynamics > of interaction are appropriate, though may lead to wrong impressions > in the mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely > for the named addressee(s) and contain confidential information. If > you are not an addressee, or responsible for delivering this email to > an addressee, you have received this email in error and are notified > that reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Jan 26 15:48:08 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 26 15:48:26 2012 Subject: [Histonet] Oil red O versus Sudan 4 In-Reply-To: <1327508472.83402.YahooMailNeo@web125403.mail.ne1.yahoo.com> References: <1327508472.83402.YahooMailNeo@web125403.mail.ne1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16D55@xmdb02.nch.kids> Candice, Remember freshly prepared Oil Red O Stock usually does not work. Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Thursday, 26 January 2012 3:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters ? ? I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and? then i stain the inside of the aorta for my plaques. ? This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. ? My question is what is the difference and what should I do differently with the Oil red o? ? Thanks so much for your help! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From one_angel_secret <@t> yahoo.com Thu Jan 26 15:48:41 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jan 26 15:48:54 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A002718833678@CVM76.vetmed.wsu.edu> References: <1327601596.45466.YahooMailNeo@web125402.mail.ne1.yahoo.com> <9EF5279EBDFE6E4FB6605E8F183A002718833678@CVM76.vetmed.wsu.edu> Message-ID: Yep! That's sounds reasonable. Maybe you could use a syringe and flush the fecal material out with formalin? This way you shouldn't have to open it up. Best of luck! Kim :D Sent from my iPhone On Jan 26, 2012, at 4:31 PM, "Truscott, Tom" wrote: > Hi Jennifer, I am by no means an expert on this, but have done a few mouse guts. I think that trying to flush out the feces with formalin or saline soon after necropsy, would help preserve the mucosa. The bacteria in the gut start breaking down the mucosa soon after death. Perhaps the gut is thin enough to fix rapidly enough to prevent damage to the mucosa without flushing. If that is the case, then flushing out the feces after fixation might help the quality of your slides. You may have to get permission to open up the gut to flush out the feces. It may hinge on how the tissue needs to be trimmed and oriented. Good luck, Tom Truscott > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes > Sent: Thursday, January 26, 2012 10:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > > Hi Histonetters! > > I have a question that I'm hoping you guys can help me with. I have a person who brings me mouse intestinal tract with fecal matter still in it. She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter. Does anyone have any suggestions as to how best to handle this?? It seems to be getting worse the further into the study she goes. > > Thank you in advance for any help that you give!! It is truly appreciated! > > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD 21205 > phone: 410-614-0131 > fax: 410-955-9677 > cell: 443-631-6361 > e-mail: jsipes1@jhmi.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Jan 26 17:01:04 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jan 26 17:01:20 2012 Subject: [Histonet] Interview Questions In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A16CE9@xmdb02.nch.kids> References: <02C099024072804EA34F5906BAC30A41062D3B@nmdamailsvr.nmda.ad.nmsu.edu>, <4940DF6D1C5FDF48931B6966AAEF93953D3FA1@chimsx08.CHI.catholichealth.net>, <3AD061FE740D464FAC7BF6B5CFB7570711F7EF79@SMCMAIL01.somerset-healthcare.com> <9963E417AAAA4CEA9E9A0713472497C4@JoePC> <6D6BD1DE8A5571489398B392A38A715760A16CE9@xmdb02.nch.kids> Message-ID: I would appreciate that Tony ----- Original Message ----- From: "Tony Henwood (SCHN)" To: "'Joe Nocito'" ; "joelle weaver" ; ; ; ; "Histonet" Sent: Thursday, January 26, 2012 3:39 PM Subject: RE: [Histonet] Interview Questions Joe, I would never wear a denim miniskirt! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, 26 January 2012 11:14 AM To: joelle weaver; trathborne@somerset-healthcare.com; billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; Histonet Subject: Re: [Histonet] Interview Questions I used to give a 10 question test on general histology. I also had the expected answers written down and on my copy. Was accused once of being a racist. What saved me was having the answers in front of me. The person didn't get one answer correct. I had a couple of embedding questions, some cutting, special stains, immunos and some QC questions. I gave the interviewee the test while I was reviewing their resume. I would also see what their facial expressions were too. I had one person tell me they didn't do specials or immunos and didn't like embedding either. When I asked if they liked filing blocks and slides, they really would rather have a lab aide do it. This person didn't have to finish the test. Too make matters worse, she wore a denim miniskirt to boot. Just my three cents Joe ----- Original Message ----- From: "joelle weaver" To: ; ; ; "Histonet" Sent: Wednesday, January 25, 2012 12:02 PM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: trathborne@somerset-healthcare.com > To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 25 Jan 2012 17:47:01 +0000 > Subject: RE: [Histonet] Interview Questions > CC: > > If your replacement will be doing actual histology, will your > institution permit the applicant to embed and cut? Can you sit down at > a multi-head scope and review slides with them? > What will the person be responsible for? Do they have experience with > all of these tasks? What would they do in a crisis situation (you can > make up one yourself that would be plausible). > People who volunteer in their personal lives, may do the same at work. > Ask how they juggle their schedule though, if there is a lot going on > in their personal lives. Be careful with how you ask these questions > though. Your HR department should be able to give you guidance in how to > phrase things. > Good luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > O'Donnell, Bill > Sent: Wednesday, January 25, 2012 12:19 PM > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Interview Questions > > It would seem that questions like "How do you feel about cannibalism?" > might also be out but might be far more helpful; than "phone" questions. > > > On the serious side, when I was much younger I hired a person who was > able to answer all the right "histo" questions and so I hired him. He > turned out to be a poser, who, shortly after I fired him showed up at > a local university with a lab coat that listed him as "Dr." He had > indeed worked in a histo lab, but as a lab assistant, and so the the > understanding of what a histologist does was well rehearsed. (BTW, it > topok me about two weeks to catch on, though the more experienced > techs in the department figured it out almost right away) > > To be fair, it was during a time in hiring history when HR departments > were not willing to give useful reference data and there were only a > handful of questions they would even ask when checking. None of them > were particularly useful or telling. For inistance, they would not ask > if the person was an histo tech, but would simply ask, did he indeed > work at your institution? > > The place where I worked required little or nothing for proof of > experience. There was no background check either. > > Today, however, reference checking is a lot easier and more reliable. > > I guess my point here is that a good reference check needs to be done > as well weeding them out by histo questions. I'm sure your HR folks > will do a fine job of this. > > Also, once you have determined that they actually have the skills, or > a realistic potential of gaining them, questions concerning dynamics > of interaction are appropriate, though may lead to wrong impressions > in the mind of the applicant. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > Sent: Wednesday, January 25, 2012 10:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Interview Questions > > So far, I am TOTALLY impressed and so grateful for your suggestions. > And here's why... did I ever tell anyone out there what the FIRST > question I was asked by the pathologist at my interview? It was..... > (wait for it....) > > > > "How do you feel about personal phone calls?". Un-freakin' believable. > I sure don't want someone to remember ME that way!!! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely > for the named addressee(s) and contain confidential information. If > you are not an addressee, or responsible for delivering this email to > an addressee, you have received this email in error and are notified > that reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From techonebs <@t> comcast.net Thu Jan 26 19:57:19 2012 From: techonebs <@t> comcast.net (Matt Mincer) Date: Thu Jan 26 19:57:38 2012 Subject: [Histonet] Precipitate Message-ID: <4F22047F.9010400@comcast.net> Hey Histonet, We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From JWeems <@t> sjha.org Thu Jan 26 23:35:29 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 26 23:35:34 2012 Subject: [Histonet] Precipitate In-Reply-To: <4F22047F.9010400@comcast.net> References: <4F22047F.9010400@comcast.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E8D9@CHEXCMS10.one.ads.che.org> Could they use DI water to test that theory? Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer Sent: Thursday, January 26, 2012 20:57 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate Hey Histonet, We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From iskaliora <@t> bioacademy.gr Fri Jan 27 01:22:00 2012 From: iskaliora <@t> bioacademy.gr (iskaliora@bioacademy.gr) Date: Fri Jan 27 01:22:10 2012 Subject: [Histonet] In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E8D9@CHEXCMS10.one.ads.che.org> References: <4F22047F.9010400@comcast.net> <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E8D9@CHEXCMS10.one.ads.che.org> Message-ID: please remove my name from the list. many thanks in advance, I. Skaliora From mw <@t> personifysearch.com Fri Jan 27 07:15:44 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Fri Jan 27 07:15:57 2012 Subject: [Histonet] 2 New Perm Histology Opportunities Message-ID: <1a817c77dcb05a27c3c4341e4a80c053@mail.gmail.com> Good Morning Histonet, Personify has had 2 new permanent Histology opportunities become available in the Northern IL area. Please contact me directly at mw@personifysearch to learn more. *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacture and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunities:* (1) QA Histologist: The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. (2) Histologist: The company currently has an opening for a Histologist to be based in Richmond IL. Salary: Based on Experience Other: Full benefits - 401k program/matching *Education and Experience Required:* - Experience with tissue grossing, tissue processing and embedding - Experience with sectioning paraffin embedded tissue as well as frozen tissue - Experience performing routine stains (H and E) as well as special stains - Experience with formulation and production of routine laboratory reagents and solutions - Experience performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Proficiency in basic computer skills and with software applications such as Microsoft Office Have a great day! Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 27 08:23:50 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jan 27 08:24:05 2012 Subject: [Histonet] Precipitate In-Reply-To: <4F22047F.9010400@comcast.net> References: <4F22047F.9010400@comcast.net> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE71E@MCINFRWEM003.ucsfmedicalcenter.org> Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse of the first 4 stations. It is the oldest processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to "get to the bottom of it" and he found the steel tubing clogged with "sand." It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes. Since then, no problems at all. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer Sent: Thursday, January 26, 2012 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate Hey Histonet, We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Jan 27 08:39:18 2012 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Jan 27 08:39:28 2012 Subject: [Histonet] Precipitate In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE71E@MCINFRWEM003.ucsfmedicalcenter.org> References: <4F22047F.9010400@comcast.net> <8D7C2D242DBD45498006B21122072BF89F5EE71E@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <44F7D4B6-DDBF-4E82-8C42-060A4583EC4D@yahoo.com> I agree with Tim, but I am curious why tap water is being used to make the 70% alcohol instead of DI water. Tap water has been known to have all kinds of contaminates. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: > Matt, we have seen that in one of our VIP5's and we do weekly hot- > water rinse of the first 4 stations. It is the oldest processor, > at about 7 years. That processor had frequent pump-in pump-out > errors randomly in the first 3 stations. Finally the service tech > decided to "get to the bottom of it" and he found the steel tubing > clogged with "sand." It took a whole day to clean it all out. It > did not look like formalin salts, but did look like the kind of > deposits that you see with hard water in pipes. > > Since then, no problems at all. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 5:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. > They are getting "sandy" clogs in station 3. The original thought > was that it was formalin salts but the texture and color was wrong. > Also, station 3 is 70% which should be weak enough. One of the > techs mentioned in passing that the water quality in their town was > really bad. I think that the problem is that, like formalin, the > alcohol is causing dissolved minerals to be released from the tap > water they use to mix their 70%. Has anyone seen this before or am > I chasing a harebrained theory? Any thoughts would be greatly > appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 27 09:17:33 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jan 27 09:17:42 2012 Subject: [Histonet] Precipitate In-Reply-To: <44F7D4B6-DDBF-4E82-8C42-060A4583EC4D@yahoo.com> References: <4F22047F.9010400@comcast.net> <8D7C2D242DBD45498006B21122072BF89F5EE71E@MCINFRWEM003.ucsfmedicalcenter.org> <44F7D4B6-DDBF-4E82-8C42-060A4583EC4D@yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE720@MCINFRWEM003.ucsfmedicalcenter.org> I was/am wondering because we do use Di water for our 80% (our lowest ETOH). But it that processor was used in a different lab for several years so maybe it got started there. None of our other 3 processors had the problem. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA From: Akemi Allison [mailto:akemiat3377@yahoo.com] Sent: Friday, January 27, 2012 6:39 AM To: Morken, Timothy Cc: 'matt@techonebiomedical.com'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Precipitate I agree with Tim, but I am curious why tap water is being used to make the 70% alcohol instead of DI water. Tap water has been known to have all kinds of contaminates. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse of the first 4 stations. It is the oldest processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to "get to the bottom of it" and he found the steel tubing clogged with "sand." It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes. Since then, no problems at all. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer Sent: Thursday, January 26, 2012 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate Hey Histonet, We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Fri Jan 27 09:48:05 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Jan 27 09:48:09 2012 Subject: [Histonet] Gloves for coverslipping? Message-ID: <4F22C735.1060104@mclean.harvard.edu> Hi Everybody: Does anyone know of a xylene-resistant glove that can be used for coverslipping, and that allows the dexterity necessary to coverslip? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From plucas <@t> biopath.org Fri Jan 27 10:10:27 2012 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Jan 27 10:08:59 2012 Subject: [Histonet] Histotech opening Orange County California Message-ID: <03F37C569B91460393E1E27893BCD837@biopath.local> We have a part time histotech position available working from 5 am on Tuesday through Saturdays. We are located in Fountain Valley, California. Please provide me a summary of your histotech experience and please send me your resume. Thank you, Paula Lucas From Alice.Fallak <@t> uhsi.org Fri Jan 27 10:41:42 2012 From: Alice.Fallak <@t> uhsi.org (Fallak, Alice) Date: Fri Jan 27 10:41:50 2012 Subject: [Histonet] FNA"S Message-ID: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice From JWeems <@t> sjha.org Fri Jan 27 10:43:26 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jan 27 10:43:35 2012 Subject: [Histonet] RE: FNA"S In-Reply-To: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> References: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E9B6@CHEXCMS10.one.ads.che.org> Radiologists obtain the specimen here - have for years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fallak, Alice Sent: Friday, January 27, 2012 11:42 To: Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FNA"S Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From trathborne <@t> somerset-healthcare.com Fri Jan 27 10:49:32 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jan 27 10:49:37 2012 Subject: [Histonet] RE: FNA"S In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E9B6@CHEXCMS10.one.ads.che.org> References: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E9B6@CHEXCMS10.one.ads.che.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7F8C4@SMCMAIL01.somerset-healthcare.com> Here too. Cytotechs are there to prepare the slides at the bedside though, and the pathologist is called when the slides are ready for an immediate interpretation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, January 27, 2012 11:43 AM To: Fallak, Alice; Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: FNA"S Radiologists obtain the specimen here - have for years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fallak, Alice Sent: Friday, January 27, 2012 11:42 To: Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FNA"S Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From TJohnson <@t> gnf.org Fri Jan 27 10:58:08 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Fri Jan 27 10:58:35 2012 Subject: [Histonet] Re: Interview Questions Message-ID: <9F3CFEE76E51B64991C7485270890B4009EF272A@EX5.lj.gnf.org> What Tony meant to say was "I would never wear a denim miniskirt! Tartan all the way, baby!" Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From vtobias <@t> uw.edu Fri Jan 27 10:58:40 2012 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri Jan 27 10:59:15 2012 Subject: [Histonet] RE: FNA"S In-Reply-To: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> References: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> Message-ID: In many cases, our Cytopathologist does the collection. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fallak, Alice Sent: Friday, January 27, 2012 8:42 AM To: Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FNA"S Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Alice.Fallak <@t> uhsi.org Fri Jan 27 11:16:52 2012 From: Alice.Fallak <@t> uhsi.org (Fallak, Alice) Date: Fri Jan 27 11:16:56 2012 Subject: [Histonet] FNA Message-ID: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B8047@KMCMAILBOX2.uhsi.local> Hi, In regard to the FNA preparation, do your pathologists prefer the smears obtained at the bedside verses the smears that are made from the pellet after centrifuging? We find that the ones prepared at the bedside are most times bloody. -Alice From Marilyn.A.Weiss <@t> kp.org Fri Jan 27 12:12:42 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Jan 27 12:13:03 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 38 In-Reply-To: <201201271803.q0RI36HH011819@cnndcsmrp021.kp.org> References: <201201271803.q0RI36HH011819@cnndcsmrp021.kp.org> Message-ID: In San Diego the radiologists do the Fna's in the CT department if they are wanting core biopsies. The Radiology tech will make the smears or the radiologist will do them. All others are done in the Head and Neck department by the Physician . All are sent to Histology to be processed and the smears to be stained. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. histonet-request@lists.utsouthwestern.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 01/27/2012 10:03 AM Please respond to histonet@lists.utsouthwestern.edu To histonet@lists.utsouthwestern.edu cc Subject Histonet Digest, Vol 98, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FNA"S (Fallak, Alice) 2. RE: FNA"S (Weems, Joyce) 3. RE: FNA"S (Rathborne, Toni) 4. Re: Interview Questions (Theresa (Teri) Johnson) 5. RE: FNA"S (Victor A. Tobias) 6. FNA (Fallak, Alice) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jan 2012 16:41:42 +0000 From: "Fallak, Alice" Subject: [Histonet] FNA"S To: "Histo net (histonet@lists.utsouthwestern.edu)" Message-ID: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B801C@KMCMAILBOX2.uhsi.local> Content-Type: text/plain; charset="us-ascii" Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice ------------------------------ Message: 2 Date: Fri, 27 Jan 2012 11:43:26 -0500 From: "Weems, Joyce" Subject: [Histonet] RE: FNA"S To: "Fallak, Alice" , "Histo net (histonet@lists.utsouthwestern.edu)" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E9B6@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Radiologists obtain the specimen here - have for years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fallak, Alice Sent: Friday, January 27, 2012 11:42 To: Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FNA"S Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Fri, 27 Jan 2012 16:49:32 +0000 From: "Rathborne, Toni" Subject: [Histonet] RE: FNA"S To: "'Weems, Joyce'" , "Fallak, Alice" , "Histo net (histonet@lists.utsouthwestern.edu)" Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7F8C4@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" Here too. Cytotechs are there to prepare the slides at the bedside though, and the pathologist is called when the slides are ready for an immediate interpretation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, January 27, 2012 11:43 AM To: Fallak, Alice; Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: FNA"S Radiologists obtain the specimen here - have for years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fallak, Alice Sent: Friday, January 27, 2012 11:42 To: Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FNA"S Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 4 Date: Fri, 27 Jan 2012 16:58:08 +0000 From: "Theresa (Teri) Johnson" Subject: [Histonet] Re: Interview Questions To: "histonet@lists.utsouthwestern.edu" Cc: "jnocito@satx.rr.com" Message-ID: <9F3CFEE76E51B64991C7485270890B4009EF272A@EX5.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" What Tony meant to say was "I would never wear a denim miniskirt! Tartan all the way, baby!" Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 5 Date: Fri, 27 Jan 2012 16:58:40 +0000 From: "Victor A. Tobias" Subject: [Histonet] RE: FNA"S To: "'Fallak, Alice'" , "'Histo net (histonet@lists.utsouthwestern.edu)'" Message-ID: Content-Type: text/plain; charset="us-ascii" In many cases, our Cytopathologist does the collection. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fallak, Alice Sent: Friday, January 27, 2012 8:42 AM To: Histo net (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FNA"S Good morning, I'm am interested in hearing the process by which other facilities obtain their FNA'S. Does the Radiologist obtain the specimen and send it down to the cytology dept. or does the histologist/cytologist assist with the collection? Thank you in advance! Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 27 Jan 2012 17:16:52 +0000 From: "Fallak, Alice" Subject: [Histonet] FNA To: "Histo net (histonet@lists.utsouthwestern.edu)" Message-ID: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B8047@KMCMAILBOX2.uhsi.local> Content-Type: text/plain; charset="us-ascii" Hi, In regard to the FNA preparation, do your pathologists prefer the smears obtained at the bedside verses the smears that are made from the pellet after centrifuging? We find that the ones prepared at the bedside are most times bloody. -Alice ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 98, Issue 38 **************************************** From tkngflght <@t> yahoo.com Fri Jan 27 12:52:55 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jan 27 12:52:59 2012 Subject: [Histonet] Cutting on an interview Message-ID: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com> ?A comment on why we have techs cut on an interview- How do you pick the BEST fit in the 60 minute interview?? The more information you gather, the more likely you'll hire the best fit.? Most of human communication is non-verbal.? Watching a potential new hire gives you SO MUCH non-verbal information in addition to validating that they know their way around a microtome. The cost of mis-hiring is ASTRONOMICAL.? 40% of companies polled say a bad hire costs over $25000.? One in four polled estimated the cost closer to $50000.? Would you really want your Aunt Minnie's GI biopsy cut by someone who COULDN'T cut a few blocks under a little new-interview pressure? An authoritative article on just this kind of interview can be read at www.fullstaff.org (A Histology Blog). It's from Career Builders and gives a lot of impirical data to the value of gathering the most information before making that hiring decision...we'd love some feedback on the post. Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org? Visit?the FREE Webblog:? www.fullstaff.org ?regarding Histology, Careers, Tricks of the Trade, New Equipment review, and much more for our industry. From one_angel_secret <@t> yahoo.com Fri Jan 27 12:54:00 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Jan 27 12:54:10 2012 Subject: [Histonet] FNA In-Reply-To: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B8047@KMCMAILBOX2.uhsi.local> References: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B8047@KMCMAILBOX2.uhsi.local> Message-ID: <01CAB2BA-BCE8-4F70-A833-69FE5172D474@yahoo.com> The ones I've known preferred the cytospin and a cell block if they were not doing a quick read. And a cell block over any smears if cells were limited. It sounds like your dealing with a training issue if the smears are too thick. Kim D Sent from my iPhone On Jan 27, 2012, at 12:16 PM, "Fallak, Alice" wrote: > Hi, > In regard to the FNA preparation, do your pathologists prefer the smears obtained at the bedside verses the smears that are made from the pellet after centrifuging? We find that the ones prepared at the bedside are most times bloody. > > -Alice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hodges420 <@t> msn.com Fri Jan 27 13:09:42 2012 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Jan 27 13:09:46 2012 Subject: [Histonet] ph of water Message-ID: Does any body have the policy to clean glasswear up to CAP standards? Could you up date me on how to do this? Thanks Tere Hodges St. Mary's Hospital From joelleweaver <@t> hotmail.com Fri Jan 27 13:14:07 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jan 27 13:14:11 2012 Subject: [Histonet] Cutting on an interview In-Reply-To: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com> References: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com> Message-ID: Thank you for sharing this link/information. I thought earlier in this thread to mention the cost factor in recruitment, hiring process, but felt it best NOT to stir this pot further after a quite lengthy response that was posted arguing against this practice. So I figured there would be some strong opionions, but just being practical, and from an organizational perspective, QUITE astronomical in terms of resources time, money, energy (to name a few). And not a good experience either for the hiree to be put into a job or culture that just doesn't suit them, so I agree you want to get it "right" whenever possible, using as many critieria as possible ( yet still remaining legal!). I continue to view a skill demonstration for any key technical duties as being similar to a typing test for wpm as used for clerical/administrative roles. But that is how I view it, and never minded being asked to perform on interviews, or even take any kind of test such as an aptitude, personality or technical knowledge type test, or answer open ended questions that demonstrated that I could think and problem-solve. I figure it is a good sign if the organization is that thorough in their hiring, it certainly shows that they will not consider just anyone, and that they have a firm grasp of who they are, what their expectations are etc... And I have alot of information right then from the interview if I have the knowledge/skills/personality to make this a good position for me. I'd rather know ahead of time than go through the new job stress, not meet the standards/expectations and have the experience of a probationary period with a poor outcome. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Fri, 27 Jan 2012 10:52:55 -0800 > From: tkngflght@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cutting on an interview > > > > A comment on why we have techs cut on an interview- > > > How do you pick the BEST fit in the 60 minute interview? The more information you gather, the more likely you'll hire the best fit. Most of human communication is non-verbal. Watching a potential new hire gives you SO MUCH non-verbal information in addition to validating that they know their way around a microtome. > > The cost of mis-hiring is ASTRONOMICAL. 40% of companies polled say a bad hire costs over $25000. One in four polled estimated the cost closer to $50000. Would you really want your Aunt Minnie's GI biopsy cut by someone who COULDN'T cut a few blocks under a little new-interview pressure? > > An authoritative article on just this kind of interview can be read at www.fullstaff.org (A Histology Blog). > > It's from Career Builders and gives a lot of impirical data to the value of gathering the most information before making that hiring decision...we'd love some feedback on the post. > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Visit the FREE Webblog: www.fullstaff.org regarding Histology, Careers, Tricks of the Trade, New Equipment review, and much more for our industry. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Jan 27 13:18:06 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jan 27 13:18:06 2012 Subject: [Histonet] Cutting on an interview In-Reply-To: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com> References: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7F963@SMCMAIL01.somerset-healthcare.com> We also have cytotech applicants look at some slides as part of an interview. These are cases which have been signed out already (patient information is protected), the applicant is given a quiet room with a scope, and records their diagnosis. Histology and Cytology are not especially large fields, and if you're interviewing someone who works fairly local, chances are that you've met them, or know someone who knows them. But with the applicant who has been out of the field for a while, or has relocated, it is helpful to have as much information as possible. Unless your job requirements are very specific, and say how many blocks/slides should be produced in a certain period of time, it will be difficult to terminate the mediocre tech, but who interviewed exceptionally well. This is also a good time to check for accuracy in labeling. Give them blocks with six- digit, non-sequential numbers, and see how they do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Friday, January 27, 2012 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting on an interview ?A comment on why we have techs cut on an interview- How do you pick the BEST fit in the 60 minute interview?? The more information you gather, the more likely you'll hire the best fit.? Most of human communication is non-verbal.? Watching a potential new hire gives you SO MUCH non-verbal information in addition to validating that they know their way around a microtome. The cost of mis-hiring is ASTRONOMICAL.? 40% of companies polled say a bad hire costs over $25000.? One in four polled estimated the cost closer to $50000.? Would you really want your Aunt Minnie's GI biopsy cut by someone who COULDN'T cut a few blocks under a little new-interview pressure? An authoritative article on just this kind of interview can be read at www.fullstaff.org (A Histology Blog). It's from Career Builders and gives a lot of impirical data to the value of gathering the most information before making that hiring decision...we'd love some feedback on the post. Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org? Visit?the FREE Webblog:? www.fullstaff.org ?regarding Histology, Careers, Tricks of the Trade, New Equipment review, and much more for our industry. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From DKBoyd <@t> chs.net Fri Jan 27 13:19:14 2012 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Jan 27 13:19:24 2012 Subject: [Histonet] FNA In-Reply-To: <2FAFABE43C2E3B4EA50D55C2A91FF63A2E1B8047@KMCMAILBOX2.uhsi.local> Message-ID: Bedside. The first drop out of the needle is usually the most diagnostic. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Fallak, Alice" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/27/2012 12:17 PM To "Histo net (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] FNA Hi, In regard to the FNA preparation, do your pathologists prefer the smears obtained at the bedside verses the smears that are made from the pellet after centrifuging? We find that the ones prepared at the bedside are most times bloody. -Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From joelleweaver <@t> hotmail.com Fri Jan 27 13:28:49 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jan 27 13:29:03 2012 Subject: [Histonet] Cutting on an interview In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F7F963@SMCMAIL01.somerset-healthcare.com> References: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com>, <3AD061FE740D464FAC7BF6B5CFB7570711F7F963@SMCMAIL01.somerset-healthcare.com> Message-ID: Yes, agree- this is essentially what I was trying to say eariler in the thread. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathborne@somerset-healthcare.com To: tkngflght@yahoo.com; histonet@lists.utsouthwestern.edu Date: Fri, 27 Jan 2012 19:18:06 +0000 Subject: RE: [Histonet] Cutting on an interview CC: We also have cytotech applicants look at some slides as part of an interview. These are cases which have been signed out already (patient information is protected), the applicant is given a quiet room with a scope, and records their diagnosis. Histology and Cytology are not especially large fields, and if you're interviewing someone who works fairly local, chances are that you've met them, or know someone who knows them. But with the applicant who has been out of the field for a while, or has relocated, it is helpful to have as much information as possible. Unless your job requirements are very specific, and say how many blocks/slides should be produced in a certain period of time, it will be difficult to terminate the mediocre tech, but who interviewed exceptionally well. This is also a good time to check for accuracy in labeling. Give them blocks with six- digit, non-sequential numbers, and see how they do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Friday, January 27, 2012 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting on an interview A comment on why we have techs cut on an interview- How do you pick the BEST fit in the 60 minute interview? The more information you gather, the more likely you'll hire the best fit. Most of human communication is non-verbal. Watching a potential new hire gives you SO MUCH non-verbal information in addition to validating that they know their way around a microtome. The cost of mis-hiring is ASTRONOMICAL. 40% of companies polled say a bad hire costs over $25000. One in four polled estimated the cost closer to $50000. Would you really want your Aunt Minnie's GI biopsy cut by someone who COULDN'T cut a few blocks under a little new-interview pressure? An authoritative article on just this kind of interview can be read at www.fullstaff.org (A Histology Blog). It's from Career Builders and gives a lot of impirical data to the value of gathering the most information before making that hiring decision...we'd love some feedback on the post. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Visit the FREE Webblog: www.fullstaff.org regarding Histology, Careers, Tricks of the Trade, New Equipment review, and much more for our industry. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LStadler <@t> cbiolabs.com Fri Jan 27 14:26:03 2012 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Fri Jan 27 14:26:07 2012 Subject: [Histonet] Exam Prep Materials Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC725A6CB9@cbiolabs05.CBiolabs.local> Has anyone ever heard of or used the "Histotechnology Exam Secrets Study Guide"http://www.mo-media.com/histotech/? If so, I would appreciate opinions on it. Thanks! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From POWELL_SA <@t> mercer.edu Fri Jan 27 14:33:54 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Jan 27 14:34:03 2012 Subject: [Histonet] RE: Exam Prep Materials In-Reply-To: <98CC14B915EBA84B9A326D45CC3C1DEC725A6CB9@cbiolabs05.CBiolabs.local> References: <98CC14B915EBA84B9A326D45CC3C1DEC725A6CB9@cbiolabs05.CBiolabs.local> Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD792F015@MERCERMAIL.MercerU.local> Found it on Amazon but not familiar with it. http://www.amazon.com/s/ref=ntt_athr_dp_sr_1?_encoding=UTF8&sort=relevancerank&search-alias=books&ie=UTF8&field-author=HTL%20Exam%20Secrets%20Test%20Prep%20Team -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lyn Stadler Sent: Friday, January 27, 2012 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Materials Has anyone ever heard of or used the "Histotechnology Exam Secrets Study Guide"http://www.mo-media.com/histotech/? If so, I would appreciate opinions on it. Thanks! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Jan 27 15:11:24 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 27 15:11:50 2012 Subject: [Histonet] Cutting on an interview In-Reply-To: References: <1327690375.96620.YahooMailNeo@web39404.mail.mud.yahoo.com>, <3AD061FE740D464FAC7BF6B5CFB7570711F7F963@SMCMAIL01.somerset-healthcare.com> Message-ID: <000601ccdd38$3be285f0$b3a791d0$@bresnan.net> I find the wording "cutting on an interview" interesting. It could mean cutting out e.g. leaving and not doing the interview. Cutting = sectioning?! Sorry if these appear to be a "cutting" remarks. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, January 27, 2012 12:29 PM To: trathborne@somerset-healthcare.com; Cheryl R. Kerry; Histonet Subject: RE: [Histonet] Cutting on an interview Yes, agree- this is essentially what I was trying to say eariler in the thread. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathborne@somerset-healthcare.com To: tkngflght@yahoo.com; histonet@lists.utsouthwestern.edu Date: Fri, 27 Jan 2012 19:18:06 +0000 Subject: RE: [Histonet] Cutting on an interview CC: We also have cytotech applicants look at some slides as part of an interview. These are cases which have been signed out already (patient information is protected), the applicant is given a quiet room with a scope, and records their diagnosis. Histology and Cytology are not especially large fields, and if you're interviewing someone who works fairly local, chances are that you've met them, or know someone who knows them. But with the applicant who has been out of the field for a while, or has relocated, it is helpful to have as much information as possible. Unless your job requirements are very specific, and say how many blocks/slides should be produced in a certain period of time, it will be difficult to terminate the mediocre tech, but who interviewed exceptionally well. This is also a good time to check for accuracy in labeling. Give them blocks with six- digit, non-sequential numbers, and see how they do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Friday, January 27, 2012 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting on an interview A comment on why we have techs cut on an interview- How do you pick the BEST fit in the 60 minute interview? The more information you gather, the more likely you'll hire the best fit. Most of human communication is non-verbal. Watching a potential new hire gives you SO MUCH non-verbal information in addition to validating that they know their way around a microtome. The cost of mis-hiring is ASTRONOMICAL. 40% of companies polled say a bad hire costs over $25000. One in four polled estimated the cost closer to $50000. Would you really want your Aunt Minnie's GI biopsy cut by someone who COULDN'T cut a few blocks under a little new-interview pressure? An authoritative article on just this kind of interview can be read at www.fullstaff.org (A Histology Blog). It's from Career Builders and gives a lot of impirical data to the value of gathering the most information before making that hiring decision...we'd love some feedback on the post. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Visit the FREE Webblog: www.fullstaff.org regarding Histology, Careers, Tricks of the Trade, New Equipment review, and much more for our industry. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gina.Rodriguez <@t> leica-microsystems.com Fri Jan 27 16:01:52 2012 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Fri Jan 27 16:01:58 2012 Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 01/31/2012) Message-ID: I am out of the office until 01/31/2012. I will respond to your message when I return. If you need immediate assistance please contact 800-248-0123 or Tech.support@leica-microsystems.com Note: This is an automated response to your message "Histonet Digest, Vol 98, Issue 37" sent on 1/27/2012 10:03:04 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From shive003 <@t> umn.edu Fri Jan 27 16:04:34 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Jan 27 16:04:37 2012 Subject: [Histonet] Mycoplasma bovis IHC Message-ID: Can anyone steer me toward a reliable vendor source for *Mycoplasma bovis*antibody (to be used on PPFE tissues). Either monoclonal or polyclonal antibodies is fine. Also, if you've used them yourself, what type of antigen retrieval worked best for you? Thanks in advance, Jan Shivers Section Head - IHC/Histology/EM UMN Veterinary Diagnostic Lab St. Paul, MN 55108 shive003@umn.edu From junk <@t> anesthesia.ucsf.edu Fri Jan 27 16:05:13 2012 From: junk <@t> anesthesia.ucsf.edu (Jun, Kristine) Date: Fri Jan 27 16:06:43 2012 Subject: [Histonet] Question Message-ID: <714FF380AA1F574DA36017DF9C3C8D9A9C87E5@ex07.net.ucsf.edu> Topic - Using the vibrating microtome with frozen tissue Question - Hello everyone. My lab recently purchased a Leica VT1000S with the intent to use it for 200 um sections of brain tissue. I am familiar with vibratome applications for fresh tissues, but my PI would like me to use it strictly for fresh frozen mouse brains (unfixed). I am having great difficulty with even obtaining one good section and the technical service representatives at Leica were unable to help me as well. I ordered injector blades from Ted Pella but, while my order is on its way, I was looking for any tips or suggestions. I keep my frozen brains on ice and fill the buffer tray with 4% PFA in PBS along with some ice around the edges, and keep dry ice around the buffer tray to keep it cold. My brain rapidly defrosts as I glue it to the stage and the moment that it hits the PFA, it starts to get gummy-looking in appearance. I have tried to section at various frequencies and speeds, but the results are still not great. If anyone has any tips or experience with this, please let me know! Thank you! Kristine Jun UCSF Center for Cerebrovascular Research 1001 Potrero Avenue, Box 1371 San Francisco, CA 94110 phone: 415-206-3344 fax: 415-206-8907 junk@anesthesia.ucsf.edu From katiejgustafson <@t> gmail.com Fri Jan 27 16:52:58 2012 From: katiejgustafson <@t> gmail.com (Katie Gustafson) Date: Fri Jan 27 16:53:01 2012 Subject: [Histonet] Online HT program graduates: How'd you do it? Questions from a future student Message-ID: Hello! I have a couple of questions for those of you who obtained your HT education through an online program. I am in the San Francisco Bay Area, and I am really interested in becoming an HT. There are no HT programs nearby (except for Kaiser employees, I believe). I plan to enroll in an online program after I obtain work as a lab assistant (I'll complete a phlebotomy program in a week or so). Here's what I'm curious about: 1. Did you seek out your first lab position with the intention of becoming an HT, or was the idea of becoming an HT something that didn't come up for you until after you began working in a lab? If you sought out your first position with the intention of becoming an HT, how did you determine that your employer would support your pursuit of an HT certification? 2. What sort of impact, if any, is there on the lab's productivity when an employee pursues an HT education after work hours, with the help of the lab's equipment and the expertise of the in-house HT? 3. How much lab experience (i.e., # of months?) would you recommend before pursuing an online HT program? Thank you so much everybody, and have a great weekend! Katie From madeleinehuey <@t> gmail.com Sat Jan 28 01:02:33 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Jan 28 01:02:43 2012 Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 37 In-Reply-To: <4f22cc5f.c3cbe00a.26f1.ffffaffcSMTPIN_ADDED@mx.google.com> References: <4f22cc5f.c3cbe00a.26f1.ffffaffcSMTPIN_ADDED@mx.google.com> Message-ID: To: Jennifer K. Sipes Here's my suggestion; 1) Perfuse the mouse with 10% NFB (their legs & tail will move during perfusion, then harden if well perfused) 2) Remove intestine out & post-fix again with formalin for overnight 3) Fill a 10cc syringe with formalin & flush everything out 4) process & cut Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Fri, Jan 27, 2012 at 8:10 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Re: Histonet Digest, Vol 98, Issue 36 (Jennifer Sipes) > ? 2. RE: Histonet Digest, Vol 98, Issue 35 (Reynolds,Donna M) > ? 3. Fwd: [Histonet] oct to paraffin (Emily Sours) > ? 4. RE: Re: Histonet Digest, Vol 98, Issue 36 (Truscott, Tom) > ? 5. RE: Interview Questions (Tony Henwood (SCHN)) > ? 6. RE: Oil red O versus Sudan 4 (Tony Henwood (SCHN)) > ? 7. Re: Re: Histonet Digest, Vol 98, Issue 36 (Kim Donadio) > ? 8. Re: Interview Questions (Joe Nocito) > ? 9. Precipitate (Matt Mincer) > ?10. RE: Precipitate (Weems, Joyce) > ?11. RE: [Histonet] (iskaliora@bioacademy.gr) > ?12. 2 New Perm Histology Opportunities (Matt Ward) > ?13. RE: Precipitate (Morken, Timothy) > ?14. Re: Precipitate (Akemi Allison) > ?15. RE: Precipitate (Morken, Timothy) > ?16. Gloves for coverslipping? (Tim Wheelock) > ?17. Histotech opening Orange County California (Paula Lucas) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 26 Jan 2012 10:13:16 -0800 (PST) > From: Jennifer Sipes > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1327601596.45466.YahooMailNeo@web125402.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Histonetters! > > I have a question that I'm hoping you guys can help me with.? I have a person who brings me mouse intestinal tract with fecal matter still in it.? She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter.? Does anyone have any suggestions as to how best to handle this??? It seems to be getting worse the further into the study she goes. > > Thank you in advance for any help that you give!! It is truly appreciated! > > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD? 21205 > phone:???? 410-614-0131 > fax:???????? 410-955-9677 > cell:???????? 443-631-6361 > e-mail:? jsipes1@jhmi.edu > > ------------------------------ > > Message: 2 > Date: Thu, 26 Jan 2012 12:17:53 -0600 > From: "Reynolds,Donna M" > Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 35 > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<785BBF0C5F49CE41BA74460A43A08F023049632196@DCPWVMBXC0VS3.mdanderson.edu> > > Content-Type: text/plain; charset="us-ascii" > > > I can definitely see some of the pitfallls to having someone cut. But it would certainly weed out anyone who didn't even know how to put a block in the microtome. ?This is also what probation periods are for to see if this person fits with your lab. > But have you ever heard of a secretary hired without a typing test ?or 9 key if that was a heavy part of their job. Even with computers today this is still done. > Donna > Houston, TX > > > Message: 3 > Date: Thu, 26 Jan 2012 02:59:49 +0000 (UTC) > From: koellingr@comcast.net > Subject: Re: [Histonet] Interview Questions > To: joelle weaver > Cc: Histonet > Message-ID: > ? ? ? ?<1983057611.84976.1327546789860.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> > > Content-Type: text/plain; charset=utf-8 > > This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say "I object"? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? > > > Ray > Seattle, WA > > > > > ------------------------------ > > Message: 3 > Date: Thu, 26 Jan 2012 14:24:46 -0500 > From: Emily Sours > Subject: Fwd: [Histonet] oct to paraffin > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=UTF-8 > > I may have missed this but does one fix the embryo again in Carnoy's or > whatever after it has been washed (assuming it was fixed in the first place) > I would think the initial para fixation would be enough and (again after > washing in aqueous solution) dehydrating it up to xylene/toluene/non-aqeous > solution before the paraffin steps. > If one did fix again, would it be washed in 50% EtOH, 70% EtOH, before the > fix? > Or is this what the NBF washes are for? I have never used NBF, except when > I tried to make Serra's with it, and yeah, that's not a good idea. ?Always > use formalin, not NBF in Serra's or you'll get a lovely milky solution > that's not fit for fixing. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that???s beautiful. > --Ron Swanson > > > > On Wed, Jan 25, 2012 at 4:11 PM, Joseph Madary wrote: > >> If you can spare some nbf the best thing to do would be to place the oct >> block right into NBF and use that as the "wash" and then move onto a fresh >> change of NBF. I would avoid straight water. NBF has enough water in it to >> rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough >> and a safe bet. >> >> Nick Madary, HT/HTL(ASCP)QIHC >> George Washington University >> Pathology Core Laboratory >> Ross Hall, Room 706 >> 23rd and I Street NW >> Washington D.C. 20037 >> 202.994.8196 >> patjnm@gwumc.edu >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 4 > Date: Thu, 26 Jan 2012 21:31:01 +0000 > From: "Truscott, Tom" > Subject: RE: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > To: Jennifer Sipes , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<9EF5279EBDFE6E4FB6605E8F183A002718833678@CVM76.vetmed.wsu.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Jennifer, I am by no means an expert on this, but have done a few mouse guts. I think that trying to flush out the feces with formalin or saline soon after necropsy, would help preserve the mucosa. The bacteria in the gut start breaking down the mucosa soon after death. Perhaps the gut is thin enough to fix rapidly enough to prevent damage to the mucosa without flushing. If that is the case, then flushing out the feces after fixation might help the quality of your slides. You may have to get permission to open up the gut to flush out the feces. It may hinge on how the tissue needs to be trimmed and oriented. Good luck, Tom Truscott > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes > Sent: Thursday, January 26, 2012 10:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > > Hi Histonetters! > > I have a question that I'm hoping you guys can help me with.? I have a person who brings me mouse intestinal tract with fecal matter still in it.? She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter.? Does anyone have any suggestions as to how best to handle this??? It seems to be getting worse the further into the study she goes. > > Thank you in advance for any help that you give!! It is truly appreciated! > > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD? 21205 > phone:???? 410-614-0131 > fax:???????? 410-955-9677 > cell:???????? 443-631-6361 > e-mail:? jsipes1@jhmi.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Thu, 26 Jan 2012 21:39:59 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] Interview Questions > To: "'Joe Nocito'" , joelle weaver > ? ? ? ?, "trathborne@somerset-healthcare.com" > ? ? ? ?, > ? ? ? ?"billodonnell@catholichealth.net" , > ? ? ? ?"sbreeden@nmda.nmsu.edu" , Histonet > ? ? ? ? > Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16CE9@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Joe, > > I would never wear a denim miniskirt! > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Thursday, 26 January 2012 11:14 AM > To: joelle weaver; trathborne@somerset-healthcare.com; billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; Histonet > Subject: Re: [Histonet] Interview Questions > > I used to give a 10 question test on general histology. I also had the expected answers written down and on my copy. Was accused once of being a racist. What saved me was having the answers in front of me. The person didn't get one answer correct. I had a couple of embedding questions, some cutting, special stains, immunos and some QC questions. I gave the interviewee the test while I was reviewing their resume. I would also see what their facial expressions were too. I had one person tell me they didn't do specials or immunos and didn't like embedding either. When I asked if they liked filing blocks and slides, they really would rather have a lab aide do it. This person didn't have to finish the test. Too make matters worse, she wore a denim miniskirt to boot. Just my three cents > > Joe > ----- Original Message ----- > From: "joelle weaver" > To: ; ; > ; "Histonet" > Sent: Wednesday, January 25, 2012 12:02 PM > Subject: RE: [Histonet] Interview Questions > > > > Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, ?as in people don't feel that they should have to "prove" they can do histology. > This perception, ?I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? ?If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > ?> From: trathborne@somerset-healthcare.com >> To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; >> histonet@lists.utsouthwestern.edu >> Date: Wed, 25 Jan 2012 17:47:01 +0000 >> Subject: RE: [Histonet] Interview Questions >> CC: >> >> If your replacement will be doing actual histology, will your >> institution permit the applicant to embed and cut? Can you sit down at >> a multi-head scope and review slides with them? >> What will the person be responsible for? Do they have experience with >> all of these tasks? What would they do in a crisis situation (you can >> make up one yourself that would be plausible). >> People who volunteer in their personal lives, may do the same at work. >> Ask how they juggle their schedule though, if there is a lot going on >> in their personal lives. Be careful with how you ask these questions >> though. Your HR department should be able to give you guidance in how to phrase things. >> Good luck. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> O'Donnell, Bill >> Sent: Wednesday, January 25, 2012 12:19 PM >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Interview Questions >> >> It would seem that questions like "How do you feel about cannibalism?" >> might also be out but might be far more helpful; than "phone" questions. >> >> >> On the serious side, when I was much younger I hired a person who was >> able to answer all the right "histo" questions and so I hired him. He >> turned out to be a poser, who, shortly after I fired him showed up at >> a local university with a lab coat that listed him as "Dr." He had >> indeed worked in a histo lab, but as a lab assistant, and so the the >> understanding of what a histologist does was well rehearsed. (BTW, it >> topok me about two weeks to catch on, though the more experienced >> techs in the department figured it out almost right away) >> >> To be fair, it was during a time in hiring history when HR departments >> were not willing to give useful reference data and there were only a >> handful of questions they would even ask when checking. None of them >> were particularly useful or telling. For inistance, they would not ask >> if the person was an histo tech, but would simply ask, did he indeed >> work at your institution? >> >> The place where I worked required little or nothing for proof of >> experience. There was no background check either. >> >> Today, however, reference checking is a lot easier and more reliable. >> >> I guess my point here is that a good reference check needs to be done >> as well weeding them out by histo questions. ?I'm sure your HR folks >> will do a fine job of this. >> >> Also, once you have determined that they actually have the skills, or >> a realistic potential of gaining them, questions concerning dynamics >> of interaction are appropriate, though may lead to wrong impressions >> in the mind of the applicant. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Breeden, Sara >> Sent: Wednesday, January 25, 2012 10:52 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Interview Questions >> >> So far, I am TOTALLY impressed and so grateful for your suggestions. >> And here's why... did I ever tell anyone out there what the FIRST >> question I was asked by the pathologist at my interview? ? It was..... >> (wait for it....) >> >> >> >> "How do you feel about personal phone calls?". ?Un-freakin' believable. >> I sure don't want someone to remember ME that way!!! >> >> >> >> Sally Breeden, HT(ASCP) >> >> New Mexico Department of Agriculture >> >> Veterinary Diagnostic Services >> >> 1101 Camino de Salud NE >> >> Albuquerque, NM ?87102 >> >> 505-383-9278 (Histology Lab) >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely >> for the named addressee(s) and contain confidential information. If >> you are not an addressee, or responsible for delivering this email to >> an addressee, you have received this email in error and are notified >> that reading, copying, or disclosing this email is prohibited. If you >> received this email in error, immediately reply to the sender and >> delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical >> Center and are intended only for the addressee. ?The information >> contained in this message is confidential and may contain privileged, >> confidential, proprietary and/or trade secret information entitled to >> protection and/or exemption from disclosure under applicable law. >> Unauthorized forwarding, printing, copying, distribution, or use of >> such information is strictly prohibited and may be unlawful. ?If you >> are not the addressee, please promptly delete this message and notify >> the sender of the delivery error by e-mail or you may call Somerset >> Medical Center's computer Help Desk at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, event >> listings, health information and more. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 6 > Date: Thu, 26 Jan 2012 21:48:08 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] Oil red O versus Sudan 4 > To: "'Candice Smoots'" , Histonet > ? ? ? ? > Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16D55@xmdb02.nch.kids> > Content-Type: text/plain; charset="iso-8859-1" > > Candice, > > Remember freshly prepared Oil Red O Stock usually does not work. > Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots > Sent: Thursday, 26 January 2012 3:21 AM > To: Histonet > Subject: [Histonet] Oil red O versus Sudan 4 > > Hi Histonetters > > > I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and? then i stain the inside of the aorta for my plaques. > > This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. > > My question is what is the difference and what should I do differently with the Oil red o? > > Thanks so much for your help! > > I remain yours truely, > > Candice Camille > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 7 > Date: Thu, 26 Jan 2012 16:48:41 -0500 > From: Kim Donadio > Subject: Re: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > To: "Truscott, Tom" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Jennifer Sipes > ? ? ? ? > Message-ID: > Content-Type: text/plain; ? ? ? charset=us-ascii > > Yep! That's sounds reasonable. ?Maybe you could use a syringe and flush the fecal material out with formalin? This way you shouldn't have to open it up. Best of luck! > Kim :D > > Sent from my iPhone > > On Jan 26, 2012, at 4:31 PM, "Truscott, Tom" wrote: > >> Hi Jennifer, I am by no means an expert on this, but have done a few mouse guts. I think that trying to flush out the feces with formalin or saline soon after necropsy, would help preserve the mucosa. The bacteria in the gut start breaking down the mucosa soon after death. Perhaps the gut is thin enough to fix rapidly enough to prevent damage to the mucosa without flushing. If that is the case, then flushing out the feces after fixation might help the quality of your slides. You may have to get permission to open up the gut to flush out the feces. It may hinge on how the tissue needs to be trimmed and oriented. Good luck, Tom Truscott >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes >> Sent: Thursday, January 26, 2012 10:13 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 >> >> Hi Histonetters! >> >> I have a question that I'm hoping you guys can help me with. ?I have a person who brings me mouse intestinal tract with fecal matter still in it. ?She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter. ?Does anyone have any suggestions as to how best to handle this?? ?It seems to be getting worse the further into the study she goes. >> >> Thank you in advance for any help that you give!! It is truly appreciated! >> >> Jennifer K. Sipes, ALAT >> Sr. Laboratory Technician >> Johns Hopkins University >> Ross 933 >> 720 Rutland Avenue >> Baltimore, MD ?21205 >> phone: ? ? 410-614-0131 >> fax: ? ? ? ? 410-955-9677 >> cell: ? ? ? ? 443-631-6361 >> e-mail: ?jsipes1@jhmi.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Thu, 26 Jan 2012 17:01:04 -0600 > From: "Joe Nocito" > Subject: Re: [Histonet] Interview Questions > To: "Tony Henwood \(SCHN\)" , ? "joelle > ? ? ? ?weaver" , > ? ? ? ?, > ? ? ? ?, ? ? ?, > ? ? ? ?"Histonet" > Message-ID: > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > ? ? ? ?reply-type=original > > I would appreciate that Tony > ----- Original Message ----- > From: "Tony Henwood (SCHN)" > To: "'Joe Nocito'" ; "joelle weaver" > ; ; > ; ; "Histonet" > > Sent: Thursday, January 26, 2012 3:39 PM > Subject: RE: [Histonet] Interview Questions > > > Joe, > > I would never wear a denim miniskirt! > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Thursday, 26 January 2012 11:14 AM > To: joelle weaver; trathborne@somerset-healthcare.com; > billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; Histonet > Subject: Re: [Histonet] Interview Questions > > I used to give a 10 question test on general histology. I also had the > expected answers written down and on my copy. Was accused once of being a > racist. What saved me was having the answers in front of me. The person > didn't get one answer correct. I had a couple of embedding questions, some > cutting, special stains, immunos and some QC questions. I gave the > interviewee the test while I was reviewing their resume. I would also see > what their facial expressions were too. I had one person tell me they didn't > do specials or immunos and didn't like embedding either. When I asked if > they liked filing blocks and slides, they really would rather have a lab > aide do it. This person didn't have to finish the test. Too make matters > worse, she wore a denim miniskirt to boot. Just my three cents > > Joe > ----- Original Message ----- > From: "joelle weaver" > To: ; ; > ; "Histonet" > Sent: Wednesday, January 25, 2012 12:02 PM > Subject: RE: [Histonet] Interview Questions > > > > Love this! I always want to do demonstration during technical interviews, > but usually get "shot down" from managers and argued with in general, ?as in > people don't feel that they should have to "prove" they can do histology. > This perception, ?I never got, because I always saw it as in a job > interview-in what other situation are you more trying to "prove" or impress > with your knowledge, attitude, skills and experience? ?If you do bench work, > you can tell in just a few minutes of observation much more information than > you could get with quite a few questions. To be fair, I take into account > nervousness, being closely observed, and lack of familiarity with equipment > etc. I don't know, I think its fair if those are important skills to the > position/role. Was not sure if Sara's job was mostly technical though, so > thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > ?> From: trathborne@somerset-healthcare.com >> To: billodonnell@catholichealth.net; sbreeden@nmda.nmsu.edu; >> histonet@lists.utsouthwestern.edu >> Date: Wed, 25 Jan 2012 17:47:01 +0000 >> Subject: RE: [Histonet] Interview Questions >> CC: >> >> If your replacement will be doing actual histology, will your >> institution permit the applicant to embed and cut? Can you sit down at >> a multi-head scope and review slides with them? >> What will the person be responsible for? Do they have experience with >> all of these tasks? What would they do in a crisis situation (you can >> make up one yourself that would be plausible). >> People who volunteer in their personal lives, may do the same at work. >> Ask how they juggle their schedule though, if there is a lot going on >> in their personal lives. Be careful with how you ask these questions >> though. Your HR department should be able to give you guidance in how to >> phrase things. >> Good luck. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> O'Donnell, Bill >> Sent: Wednesday, January 25, 2012 12:19 PM >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Interview Questions >> >> It would seem that questions like "How do you feel about cannibalism?" >> might also be out but might be far more helpful; than "phone" questions. >> >> >> On the serious side, when I was much younger I hired a person who was >> able to answer all the right "histo" questions and so I hired him. He >> turned out to be a poser, who, shortly after I fired him showed up at >> a local university with a lab coat that listed him as "Dr." He had >> indeed worked in a histo lab, but as a lab assistant, and so the the >> understanding of what a histologist does was well rehearsed. (BTW, it >> topok me about two weeks to catch on, though the more experienced >> techs in the department figured it out almost right away) >> >> To be fair, it was during a time in hiring history when HR departments >> were not willing to give useful reference data and there were only a >> handful of questions they would even ask when checking. None of them >> were particularly useful or telling. For inistance, they would not ask >> if the person was an histo tech, but would simply ask, did he indeed >> work at your institution? >> >> The place where I worked required little or nothing for proof of >> experience. There was no background check either. >> >> Today, however, reference checking is a lot easier and more reliable. >> >> I guess my point here is that a good reference check needs to be done >> as well weeding them out by histo questions. ?I'm sure your HR folks >> will do a fine job of this. >> >> Also, once you have determined that they actually have the skills, or >> a realistic potential of gaining them, questions concerning dynamics >> of interaction are appropriate, though may lead to wrong impressions >> in the mind of the applicant. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Breeden, Sara >> Sent: Wednesday, January 25, 2012 10:52 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Interview Questions >> >> So far, I am TOTALLY impressed and so grateful for your suggestions. >> And here's why... did I ever tell anyone out there what the FIRST >> question I was asked by the pathologist at my interview? ? It was..... >> (wait for it....) >> >> >> >> "How do you feel about personal phone calls?". ?Un-freakin' believable. >> I sure don't want someone to remember ME that way!!! >> >> >> >> Sally Breeden, HT(ASCP) >> >> New Mexico Department of Agriculture >> >> Veterinary Diagnostic Services >> >> 1101 Camino de Salud NE >> >> Albuquerque, NM ?87102 >> >> 505-383-9278 (Histology Lab) >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely >> for the named addressee(s) and contain confidential information. If >> you are not an addressee, or responsible for delivering this email to >> an addressee, you have received this email in error and are notified >> that reading, copying, or disclosing this email is prohibited. If you >> received this email in error, immediately reply to the sender and >> delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical >> Center and are intended only for the addressee. ?The information >> contained in this message is confidential and may contain privileged, >> confidential, proprietary and/or trade secret information entitled to >> protection and/or exemption from disclosure under applicable law. >> Unauthorized forwarding, printing, copying, distribution, or use of >> such information is strictly prohibited and may be unlawful. ?If you >> are not the addressee, please promptly delete this message and notify >> the sender of the delivery error by e-mail or you may call Somerset >> Medical Center's computer Help Desk at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, event >> listings, health information and more. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Thu, 26 Jan 2012 19:57:19 -0600 > From: Matt Mincer > Subject: [Histonet] Precipitate > To: histonet@lists.utsouthwestern.edu > Message-ID: <4F22047F.9010400@comcast.net> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They > are getting "sandy" clogs in station 3. The original thought was that it > was formalin salts but the texture and color was wrong. Also, station 3 > is 70% which should be weak enough. One of the techs mentioned in > passing that the water quality in their town was really bad. I think > that the problem is that, like formalin, the alcohol is causing > dissolved minerals to be released from the tap water they use to mix > their 70%. Has anyone seen this before or am I chasing a harebrained > theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > > > ------------------------------ > > Message: 10 > Date: Fri, 27 Jan 2012 00:35:29 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Precipitate > To: "matt@techonebiomedical.com" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<92AD9B20A6C38C4587A9FEBE3A30E1640846C1E8D9@CHEXCMS10.one.ads.che.org> > Content-Type: text/plain; charset="us-ascii" > > Could they use DI water to test that theory? > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376?- Phone > 678-843-7831?- Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 20:57 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. ?Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > > > ------------------------------ > > Message: 11 > Date: Fri, 27 Jan 2012 09:22:00 +0200 > From: iskaliora@bioacademy.gr > Subject: RE: [Histonet] > To: histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain;charset=iso-8859-7 > > please remove my name from the list. > many thanks in advance, > I. Skaliora > > > > > ------------------------------ > > Message: 12 > Date: Fri, 27 Jan 2012 08:15:44 -0500 > From: Matt Ward > Subject: [Histonet] 2 New Perm Histology Opportunities > To: histonet@lists.utsouthwestern.edu > Message-ID: <1a817c77dcb05a27c3c4341e4a80c053@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Good Morning Histonet, > > > > Personify has had 2 new permanent Histology opportunities become available > in the Northern IL area. > > > > Please contact me directly at mw@personifysearch to learn more. > > > > *The Company:* > > Well-established provider of consumables and medical device accessories for > clinical histology and research laboratories. ?The facility works closely > with our UK, German and Australian facilities in the development, > manufacture and marketing of products including processing reagents, > storage and specimen transport devices, cytology accessories and safety > products. > > This is a globally focused business with significant sales and operations > in the US, Europe and Asia Pacific as well as a direct presence in over 100 > countries. > > *The Opportunities:* > > (1) ? ?QA Histologist: The company currently has an opening for a Quality > Assurance Histologist to be based in Richmond IL. > > (2) ? ?Histologist: The company currently has an opening for a Histologist > to be based in Richmond IL. > > Salary: Based on Experience > > Other: Full benefits - 401k program/matching > > > *Education and Experience Required:* > > - Experience with tissue grossing, tissue processing and embedding > - Experience with sectioning paraffin embedded tissue as well as frozen > tissue > - Experience performing routine stains (H and E) as well as special stains > - Experience with formulation and production of routine laboratory reagents > and solutions > - Experience performing and documenting routine laboratory procedures > - Familiarity with compliance requirements in the medical device industry > - Proficiency in basic computer skills and with software applications such > as Microsoft Office > > Have a great day! > > > > Regards, > > > > > > Matt Ward > > *Account Executive* > > *Personify* > > 5020 Weston Parkway Suite 315 > > Cary NC 27513 > > (Tel) 800.875.6188 direct ext 103 > > (Fax) 919.460.0642 > > ?www.personifysearch.com > > > ------------------------------ > > Message: 13 > Date: Fri, 27 Jan 2012 06:23:50 -0800 > From: "Morken, Timothy" > Subject: RE: [Histonet] Precipitate > To: "'matt@techonebiomedical.com'" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<8D7C2D242DBD45498006B21122072BF89F5EE71E@MCINFRWEM003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse of the first 4 stations. It is the oldest ?processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to "get to the bottom of it" and he found the steel tubing clogged with "sand." It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes. > > Since then, no problems at all. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 5:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Fri, 27 Jan 2012 06:39:18 -0800 > From: Akemi Allison > Subject: Re: [Histonet] Precipitate > To: "Morken, Timothy" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?"'matt@techonebiomedical.com'" > ? ? ? ? > Message-ID: <44F7D4B6-DDBF-4E82-8C42-060A4583EC4D@yahoo.com> > Content-Type: text/plain; ? ? ? charset=US-ASCII; ? ? ? delsp=yes; ? ? ?format=flowed > > I agree with Tim, but I am curious why tap water is being used to > make the 70% alcohol instead of DI water. ?Tap water has been known > to have all kinds of contaminates. > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: > >> Matt, we have seen that in one of our VIP5's and we do weekly hot- >> water rinse of the first 4 stations. It is the oldest ?processor, >> at about 7 years. That processor had frequent pump-in pump-out >> errors randomly in the first 3 stations. Finally the service tech >> decided to "get to the bottom of it" and he found the steel tubing >> clogged with "sand." It took a whole day to clean it all out. It >> did not look like formalin salts, but did look like the kind of >> deposits that you see with hard water in pipes. >> >> Since then, no problems at all. >> >> Tim Morken >> Supervisor, Histology, IPOX >> UCSF Medical Center >> San Francisco, CA, USA >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >> bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer >> Sent: Thursday, January 26, 2012 5:57 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Precipitate >> >> Hey Histonet, >> >> We have a client who is having an odd problem with their processor. >> They are getting "sandy" clogs in station 3. The original thought >> was that it was formalin salts but the texture and color was wrong. >> Also, station 3 is 70% which should be weak enough. One of the >> techs mentioned in passing that the water quality in their town was >> really bad. I think that the problem is that, like formalin, the >> alcohol is causing dissolved minerals to be released from the tap >> water they use to mix their 70%. Has anyone seen this before or am >> I chasing a harebrained theory? Any thoughts would be greatly >> appreciated. >> >> Thanks >> Matt >> >> -- >> Matthew Mincer >> Tech One Biomedical Services >> 159 N Marion Street, PMB163 >> Oak Park, IL 60301 >> (708) 383-6040 X 10 >> fax (708) 383-6045 >> cell (708) 822-3738 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 15 > Date: Fri, 27 Jan 2012 07:17:33 -0800 > From: "Morken, Timothy" > Subject: RE: [Histonet] Precipitate > To: "'Akemi Allison'" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?"'matt@techonebiomedical.com'" > ? ? ? ? > Message-ID: > ? ? ? ?<8D7C2D242DBD45498006B21122072BF89F5EE720@MCINFRWEM003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > I was/am wondering because we do use Di water for our 80% (our lowest ETOH). But it that processor was used in a different lab for several years so maybe it got started there. > > None of our other 3 processors had the problem. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > From: Akemi Allison [mailto:akemiat3377@yahoo.com] > Sent: Friday, January 27, 2012 6:39 AM > To: Morken, Timothy > Cc: 'matt@techonebiomedical.com'; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Precipitate > > I agree with Tim, but I am curious why tap water is being used to make the 70% alcohol instead of DI water. ?Tap water has been known to have all kinds of contaminates. > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: > > > Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse of the first 4 stations. It is the oldest ?processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to "get to the bottom of it" and he found the steel tubing clogged with "sand." It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes. > > Since then, no problems at all. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 5:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Fri, 27 Jan 2012 10:48:05 -0500 > From: Tim Wheelock > Subject: [Histonet] Gloves for coverslipping? > To: histonet@lists.utsouthwestern.edu > Message-ID: <4F22C735.1060104@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi Everybody: > > Does anyone know of a xylene-resistant glove that can be used for > coverslipping, and that allows the dexterity necessary to coverslip? > > Thanks, > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > > ------------------------------ > > Message: 17 > Date: Fri, 27 Jan 2012 08:10:27 -0800 > From: "Paula Lucas" > Subject: [Histonet] Histotech opening Orange County California > To: > Message-ID: <03F37C569B91460393E1E27893BCD837@biopath.local> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > We have a part time histotech position available working from 5 am on > Tuesday through Saturdays. ?We are located in Fountain Valley, California. > > Please provide me a summary of your histotech experience and please send me > your resume. > > Thank you, > > Paula Lucas > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 37 > **************************************** From Laura.Miller <@t> leica-microsystems.com Sat Jan 28 04:00:54 2012 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Sat Jan 28 04:01:14 2012 Subject: [Histonet] AUTO: Laura Miller is Out of the Office. (returning 02/01/2012) Message-ID: I am out of the office until 02/01/2012. I am at the Leica National Sales meeting until February 1st. I will have very limited time to return emails. Thanks. Note: This is an automated response to your message "Histonet Digest, Vol 98, Issue 39" sent on 1/28/2012 1:01:13 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From rsrichmond <@t> gmail.com Sat Jan 28 08:03:08 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 28 08:03:14 2012 Subject: [Histonet] Re: FNA Message-ID: Needle biopsies, whether tissue core or FNA, require a great deal of co-operation and interplay among pathologist, cytotechnologist, and radiologist. Just how this works depends on the skills of the people involved. For example, one place I work has a senior pathologist who is highly skilled at performing and interpreting FNA's. We have no in-house cytotechnologist there, and the radiologists are from a large group that rotates through on a one-day basis from a distant site, so we never know what radiologist we'll have. At another place, the pathologist is rarely available, but the cytotechnologist is highly skilled at assisting a radiologist or other clinicians. The in-house radiologist is also highly skilled, but we have to work with a minimum of clinical information. In all of this, the needs of the individual patient need to be considered. A frequent issue: does part of the specimen need to be sent out for flow cytometry? Is unfixed tissue needed for some special cancer study? And there's the uh-oh moment when you aspirate pus and find out that what's needed is cultures. This is one of those situations where things aren't going to get better until the bean counters figure out that our lack of access to information is costing big bucks. At age 73, I don't expect to see it. Bob Richmond Samurai Pathologist Knoxville TN From koellingr <@t> comcast.net Sat Jan 28 12:22:35 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jan 28 12:22:52 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletion disinterested In-Reply-To: <1389890959.173297.1327764426860.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <1923759528.178102.1327774955299.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. Ray Phenopath Labs Seattle WA From tjasper <@t> copc.net Sat Jan 28 15:25:40 2012 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Jan 28 15:25:59 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested References: <1923759528.178102.1327774955299.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <90354A475B420441B2A0396E5008D49692C087@copc-sbs.COPC.local> Ray, Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. Kind regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Saturday, January 28, 2012 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. Ray Phenopath Labs Seattle WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Sat Jan 28 20:18:01 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jan 28 20:18:18 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletion disinterested In-Reply-To: Message-ID: <559987550.188582.1327803481007.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Lynn, Perhaps the most provocative and ingenious idea yet. Have your "competency to cut" with you; like many other files, a drivers license or others for example, follow you through (working histology) life. Excellent!!!!!!!!! Ray Seattle, WA ----- Original Message ----- From: "Lynn Dike" To: koellingr@comcast.net Sent: Saturday, January 28, 2012 5:36:58 PM Subject: RE: [Histonet] interview cutting-OT-disarmingly long for deletion disinterested I find it amazing that there is so much controversy over having a potential new hire cut during an interview. I agree with all the comments for and against. But because of CAP regulations all of us techs have to do competencies each year to prove we can use a microtome, cut a slide and stain it...also have to prove we can embed and fill a water bath. Many of us have been doing it for 30+ years and have been cutting slides which our Doctors are looking at and making diagnosis's from every day....and yet we have to have someone watch to see if we can line up a block, shave it, cut it, lay out a ribbon and pick up a section on a correctly numbered slide. Experienced techs have to do it every year and should have it in their employee file. Why not just bring a copy of the signed competencies and save the time of doing it at the interview. Lynn > Date: Sat, 28 Jan 2012 18:22:35 +0000 > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interview cutting-OT-disarmingly long for deletion disinterested > > > > > > > > > Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. > > > While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. > > > Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. > > > Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. > > > Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. > > > Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. > > > In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. > > > If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. > > > One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. > > > Ray > Phenopath Labs > Seattle WA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sun Jan 29 12:12:09 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Jan 29 12:12:14 2012 Subject: [Histonet] Re: interview.... Message-ID: Ray Koelling asked me: >>If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview.<< I really have no idea how many slides. In a normal year I sign out about 3,000 histology cases (remember I don't work full time) averaging maybe 3 slides per case. Generally I've gotten jobs, both private clients and agency clients, by recommendation. A number of years ago I was interviewed by a four-pathologist hospital group who handed me a tray of 20 slides with the necessary historical information, and was told that this was a set the group had collected, including very straightforward cases, cases with serious diagnostic pitfalls, and some cases they'd never been able to make a diagnosis on. They tried to make it a test of judgment rather than simple diagnostic skill. Told to take as much time as I needed. I guess I passed - by coincidence, the entire group chanced to break up very quickly, and an entirely different team took over. Bob Richmond Samurai Pathologist Knoxville TN From tony.henwood <@t> health.nsw.gov.au Sun Jan 29 15:54:21 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Jan 29 15:54:36 2012 Subject: [Histonet] RE: Interview Questions In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009EF272A@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009EF272A@EX5.lj.gnf.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A173FC@xmdb02.nch.kids> Absolutely (Whoops I did it again!!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Theresa (Teri) Johnson [mailto:TJohnson@gnf.org] Sent: Saturday, 28 January 2012 3:58 AM To: histonet@lists.utsouthwestern.edu Cc: jnocito@satx.rr.com; Tony Henwood (SCHN) Subject: Re: Interview Questions What Tony meant to say was "I would never wear a denim miniskirt! Tartan all the way, baby!" Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Diane.Tokugawa <@t> kp.org Sun Jan 29 18:01:47 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Sun Jan 29 18:02:07 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 01/27/2012 and will not return until 02/01/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From member <@t> linkedin.com Mon Jan 30 08:37:16 2012 From: member <@t> linkedin.com (Judith McKinney via LinkedIn) Date: Mon Jan 30 08:37:23 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <814692035.14097330.1327934236846.JavaMail.app@ela4-app0135.prod> LinkedIn ------------ Judith McKinney requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Judith Accept invitation from Judith McKinney http://www.linkedin.com/e/yvpgd1-gy1lraui-16/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I217691482_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOe3gNejoTcj99bOR2pj1vmlB4bPcNcjcQc38PczkLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=3YWPLzoB0dJB41 View invitation from Judith McKinney http://www.linkedin.com/e/yvpgd1-gy1lraui-16/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I217691482_13/3cNnP8Ud34VdzsNcAALqnpPbOYWrSlI/svi/?hs=false&tok=3gEYT3L3YdJB41 ------------------------------------------ Why might connecting with Judith McKinney be a good idea? Judith McKinney's connections could be useful to you: After accepting Judith McKinney's invitation, check Judith McKinney's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From cforster <@t> umn.edu Mon Jan 30 11:47:48 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jan 30 11:47:48 2012 Subject: [Histonet] Need feed back from any users out there! Message-ID: <4F26D7C4.4020902@umn.edu> Hello Histoland, Can anyone who may have used or is using this piece of equipment give me their feed back? We are considering it to do an "online" dewaxing and retrievel with the Biocare Nemesis stainer. Thanks in advacne for your comments! Colleen Forster HT(ASCP)QIHC U of MN Lab Vision* PT Module The Thermo Scientific* PT Module is designed to simultaneously perform dewaxing and antigen retrieval on slides prior to immunohistochemical staining. The Thermo Scientific PT Module is the benchmark for simplification, standardization and consistency of antigen retrieval procedures in IHC. The PT Module fully automates simultaneous dewaxing and antigen retrieval of IHC and includes PT Monitor software for printing/maintenance of regulatory compliant procedural reports. From kmerriam2003 <@t> yahoo.com Mon Jan 30 12:09:49 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Jan 30 12:09:56 2012 Subject: [Histonet] Need feed back from any users out there! In-Reply-To: <4F26D7C4.4020902@umn.edu> References: <4F26D7C4.4020902@umn.edu> Message-ID: <1327946989.33007.YahooMailNeo@web130105.mail.mud.yahoo.com> I wouldn't put xylene on my Nemesis stainer, it might ruin some of the parts of the instrument.? Also, the Nemesis is all room temp, you can't do HIER on it (but you can do enzymes on it). ? My 2-cents. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Colleen Forster To: Histonet Sent: Monday, January 30, 2012 12:47 PM Subject: [Histonet] Need feed back from any users out there! ? Hello Histoland, Can anyone who may have used or is using this piece of equipment give me their feed back? We are considering it to do an "online" dewaxing and retrievel with the Biocare Nemesis stainer.? Thanks in advacne for your comments! Colleen Forster HT(ASCP)QIHC U of MN ? Lab Vision* PT Module The Thermo Scientific* PT Module is designed to simultaneously perform dewaxing and antigen retrieval on slides prior to immunohistochemical staining. The Thermo Scientific PT Module is the benchmark for simplification, standardization and consistency of antigen retrieval procedures in IHC. The PT Module fully automates simultaneous dewaxing and antigen retrieval of IHC and includes PT Monitor software for printing/maintenance of regulatory compliant procedural reports. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Mon Jan 30 12:19:47 2012 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Jan 30 12:19:47 2012 Subject: [Histonet] Carbon 14 Message-ID: <018401ccdf7b$c07b9dc0$4172d940$@masshistology.com> Does anyone have any experience with tissue injected with carbon 14? I have some questions about precautions and disposal. Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From David.Burk <@t> pbrc.edu Mon Jan 30 12:20:24 2012 From: David.Burk <@t> pbrc.edu (David Burk) Date: Mon Jan 30 12:20:43 2012 Subject: [Histonet] Processing adipose tissue Message-ID: <281CF76A690FA74783D6EF0EC4EF243C215F30@pbrcas30.pbrc.edu> Esteemed experts, We have many clients who want to process mouse and human adipose tissue and are having some quality issues in the resultant slides. We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our automated processor (Excelsior) as follows: Tissue fixed for ~24 hrs in 10% NBF 70% Isopropyl alcohol (IPA) for 3 hrs 90% IPA, 3hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3hrs Paraffin, 3 hrs Paraffin, 3 hrs Paraffin, 3 hrs Embed and section at 5 um prior to H&E. An example of what the sections look like can be found here http://imgur.com/7RTGR . We also ran a sample on a "traditional overnight" EtOH/Xylene processor (not at our facility) to compare results. That image is here: http://imgur.com/GjJPg . What is obvious is that the membranes in the IPA processed tissue seem to "flap over" and don't look as crisp as the Xylene processed tissue. We did notice structural defects in both samples (not shown) typically toward the middle of the specimens. Does anyone know what is causing our IPA processed fat to have these "wide membrane" artifacts? We are going to repeat the process with an additional 30 minutes per step and raise the temperature of the steps to ~ 35 C. We are also going to cut the blocks at 2-3 um to see if it can reduce the appearance of the membranes. Thanks very much for any advice you may have for us. We are pretty locked in to using xylene-free processing methodology if at all possible but will entertain any suggestions you may have. If I can provide any further details about what we are doing on our end, please let me know and I'll be happy to provide them. Best, David Burk From rjbuesa <@t> yahoo.com Mon Jan 30 12:55:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 30 12:56:02 2012 Subject: [Histonet] Carbon 14 In-Reply-To: <018401ccdf7b$c07b9dc0$4172d940$@masshistology.com> Message-ID: <1327949751.83583.YahooMailClassic@web162103.mail.bf1.yahoo.com> Usually the amounts are very small, but always treat the tissues with the adequate precautions. Your radiology department can inform you of the current regulations in your area. Ren? J. --- On Mon, 1/30/12, jstaruk wrote: From: jstaruk Subject: [Histonet] Carbon 14 To: "'Histonet'" Date: Monday, January 30, 2012, 1:19 PM Does anyone have any experience with tissue injected with carbon 14?? I have some questions about precautions and disposal. Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com ???www.nehorselabs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Mon Jan 30 13:13:11 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Mon Jan 30 13:13:19 2012 Subject: [Histonet] Interviewing Histotechs... In-Reply-To: References: Message-ID: I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. Jerry Ricks Research Scientist University of Washington Department of Pathology histonet@lists.utsouthwestern.edu > Date: Sun, 29 Jan 2012 13:12:09 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: interview.... > > Ray Koelling asked me: > > >>If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview.<< > > I really have no idea how many slides. In a normal year I sign out > about 3,000 histology cases (remember I don't work full time) > averaging maybe 3 slides per case. > > Generally I've gotten jobs, both private clients and agency clients, > by recommendation. A number of years ago I was interviewed by a > four-pathologist hospital group who handed me a tray of 20 slides with > the necessary historical information, and was told that this was a set > the group had collected, including very straightforward cases, cases > with serious diagnostic pitfalls, and some cases they'd never been > able to make a diagnosis on. They tried to make it a test of judgment > rather than simple diagnostic skill. Told to take as much time as I > needed. I guess I passed - by coincidence, the entire group chanced to > break up very quickly, and an entirely different team took over. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Jan 30 13:30:25 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Jan 30 13:30:33 2012 Subject: [Histonet] SSTR1 - 5 antibody Message-ID: <3614531DE701422F9902A473E752A4CD@dielangs.at> Hi! Can someone give a recommendation for SSTR1 to SSTR5 antibodies for human FFPE immunohistochemistry? synonym: Somatostatin-Receptor 1 many thanks Gudrun Lang histolab, Linz, Austria From mhale <@t> carisls.com Mon Jan 30 14:01:48 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Jan 30 14:02:01 2012 Subject: [Histonet] Great Louisiana Positon Message-ID: <6F33D8418806044682A391273399860F0BA01493@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From koellingr <@t> comcast.net Mon Jan 30 14:49:05 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Jan 30 14:49:23 2012 Subject: [Histonet] Interviewing Histotechs... In-Reply-To: Message-ID: <1062883471.239715.1327956545669.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> I hope there is at least a bit more to clinical histology than quantity and speed. Maybe I know the particular high schooler you mentioned who was so good since I mentored that Mercer program/class for years and years and even brought in class-wide IHC hands-on projects. Before the teacher left and the program collapsed. Ray Seattle, WA ----- Original Message ----- From: "Jerry Ricks" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 30, 2012 11:13:11 AM Subject: [Histonet] Interviewing Histotechs... I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. Jerry Ricks Research Scientist University of Washington Department of Pathology histonet@lists.utsouthwestern.edu > Date: Sun, 29 Jan 2012 13:12:09 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: interview.... > > Ray Koelling asked me: > > >>If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview.<< > > I really have no idea how many slides. In a normal year I sign out > about 3,000 histology cases (remember I don't work full time) > averaging maybe 3 slides per case. > > Generally I've gotten jobs, both private clients and agency clients, > by recommendation. A number of years ago I was interviewed by a > four-pathologist hospital group who handed me a tray of 20 slides with > the necessary historical information, and was told that this was a set > the group had collected, including very straightforward cases, cases > with serious diagnostic pitfalls, and some cases they'd never been > able to make a diagnosis on. They tried to make it a test of judgment > rather than simple diagnostic skill. Told to take as much time as I > needed. I guess I passed - by coincidence, the entire group chanced to > break up very quickly, and an entirely different team took over. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kasaim <@t> mail.nih.gov Mon Jan 30 15:50:18 2012 From: kasaim <@t> mail.nih.gov (Kasai, Miki (NIH/NCI) [E]) Date: Mon Jan 30 15:51:24 2012 Subject: [Histonet] Immunofluorescence staining/minimizing background staining Message-ID: Hi, We are performing some immunofluorescence staining on mouse lung tissue. We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize the amount of background staining we are getting. We are titering our primary antibodies to find out optimal Ab concentration as well as the secondary conjugate Ab with the fluorophore of interest. We use donkey serum for general blocking. Any other suggestions? Much appreciation, Miki From twheelock <@t> mclean.harvard.edu Mon Jan 30 16:01:35 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Mon Jan 30 16:01:50 2012 Subject: [Histonet] Thank you Message-ID: <4F27133F.4080506@mclean.harvard.edu> Hi All: I want to thank everyone for the advice on xylene-resistant gloves. It was really helpful. Tim Wheelock Tim Wheelock Assistant Director Harvard Brain Tissue Resource Center Instructor In Neuroanatomy 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 From rosenfeldtek <@t> hotmail.com Mon Jan 30 18:15:17 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Mon Jan 30 18:15:21 2012 Subject: [Histonet] Processing adipose tissue In-Reply-To: <281CF76A690FA74783D6EF0EC4EF243C215F30@pbrcas30.pbrc.edu> References: <281CF76A690FA74783D6EF0EC4EF243C215F30@pbrcas30.pbrc.edu> Message-ID: Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to remove the IPA." I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes. http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&ved=0CGMQFjAJ&url=http%3A%2F%2Fwww.ebsciences.com%2Fpapers%2FMineral%2520oil%2520as%2520xylene%2520substitute.pdf&ei=ny4nT73bM8axiQLk3dmQAQ&usg=AFQjCNFw894if_bFAYgv6Uurw2MkO3Th5A Jerry > Date: Mon, 30 Jan 2012 12:20:24 -0600 > From: David.Burk@pbrc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing adipose tissue > > Esteemed experts, > > > > We have many clients who want to process mouse and human adipose tissue > and are having some quality issues in the resultant slides. > > We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our > automated processor (Excelsior) as follows: > > Tissue fixed for ~24 hrs in 10% NBF > > 70% Isopropyl alcohol (IPA) for 3 hrs > > 90% IPA, 3hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > > > Embed and section at 5 um prior to H&E. An example of what the sections > look like can be found here http://imgur.com/7RTGR . > > We also ran a sample on a "traditional overnight" EtOH/Xylene processor > (not at our facility) to compare results. That image is here: > http://imgur.com/GjJPg . > > What is obvious is that the membranes in the IPA processed tissue seem > to "flap over" and don't look as crisp as the Xylene processed tissue. > > We did notice structural defects in both samples (not shown) typically > toward the middle of the specimens. > > > > Does anyone know what is causing our IPA processed fat to have these > "wide membrane" artifacts? > > We are going to repeat the process with an additional 30 minutes per > step and raise the temperature of the steps to ~ 35 C. > > We are also going to cut the blocks at 2-3 um to see if it can reduce > the appearance of the membranes. > > > > Thanks very much for any advice you may have for us. We are pretty > locked in to using xylene-free processing methodology if at all possible > but will entertain any suggestions you may have. > > If I can provide any further details about what we are doing on our end, > please let me know and I'll be happy to provide them. > > > > Best, > > David Burk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Jan 30 21:01:05 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Jan 30 21:01:19 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested In-Reply-To: <90354A475B420441B2A0396E5008D49692C087@copc-sbs.COPC.local> References: <1923759528.178102.1327774955299.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> <90354A475B420441B2A0396E5008D49692C087@copc-sbs.COPC.local> Message-ID: <4CB5B8BE-6A92-4E6A-9935-9B118CFC768A@yahoo.com> Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) I don't think it should be made a big deal. You take a drivers test to drive. Peoples lives are on the line in each case. Does that a lone mean I don't hire them. Probaly not. I just need to know how much personal investment of my time I am going to need to give ...... Runs for her pillow of dreams :). Nite nite Kim Sent from my iPhone On Jan 28, 2012, at 4:25 PM, "Thomas Jasper" wrote: > Ray, > > Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. > > Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. > > Kind regards, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, OR 97701 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net > Sent: Saturday, January 28, 2012 10:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > > > > > > > > Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. > > > While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. > > > Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. > > > Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. > > > Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. > > > Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. > > > In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. > > > If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. > > > One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. > > > Ray > Phenopath Labs > Seattle WA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Cremer <@t> med.kuleuven.be Tue Jan 31 04:56:39 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Tue Jan 31 05:00:16 2012 Subject: [Histonet] Coverslipping for excellent optical quality Message-ID: Dear Histonetters, I am currently reading up on proper use and maintenance of microscopes, and inevitably stumbled across K?hler's illumination. No one ever explained this to me, although I have a 'microscopy protocol' that comes close to properly explaining the correct setup. So now that I know how to properly use a microscope fitted with such a system, there is still the issue of the coverslip/mounting medium which are considered the 'first lens'. I understand why there is a narrow tolerance for coverslip/mounting medium thickness to achieve optimal performance, but how do you control that thickness? Currently, when I mount a slide, I keep it wetted with xylene, put a sufficient amount of mounting medium on the coverslip and mount in the usual 'rolling' motion. I then push on the coverslip to remove trapped bubbles (if any) and be sure to spread the glue under the entire coverslip. However, how can one be sure about the thickness of the mounting medium layer? Using more/less glue and applying more/less pressure to the coverslip must surely influence this? Especially since we're talking microns. Does anybody have any insights or tips, or I am just nitpicking and trying to achieve an impossible result? :) Regards, Jonathan --- Jonathan Cremer Laboratory Technician Gastro-enterologie KU Leuven, Belgium From akbitting <@t> geisinger.edu Tue Jan 31 07:36:53 2012 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Jan 31 07:37:09 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested In-Reply-To: <4CB5B8BE-6A92-4E6A-9935-9B118CFC768A@yahoo.com> References: <1923759528.178102.1327774955299.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> <90354A475B420441B2A0396E5008D49692C087@copc-sbs.COPC.local> <4CB5B8BE-6A92-4E6A-9935-9B118CFC768A@yahoo.com> Message-ID: <4F27A825.2B7F.00C9.1@geisinger.edu> I've had a temp, who we interviewed over the phone, come in and sit down at a microtome and create the most horrendous slides I've ever seen. He lasted a week and we sent him back from whence he came. I don't think he was EVER a Histotech or if he was it was many, many moons ago. Point is.....he snowed us all during the interview. Just thought I'd throw that out there. >>> Kim Donadio 1/30/2012 10:01 PM >>> Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) I don't think it should be made a big deal. You take a drivers test to drive. Peoples lives are on the line in each case. Does that a lone mean I don't hire them. Probaly not. I just need to know how much personal investment of my time I am going to need to give ...... Runs for her pillow of dreams :). Nite nite Kim Sent from my iPhone On Jan 28, 2012, at 4:25 PM, "Thomas Jasper" wrote: > Ray, > > Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. > > Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. > > Kind regards, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, OR 97701 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net > Sent: Saturday, January 28, 2012 10:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > > > > > > > > Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. > > > While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. > > > Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. > > > Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. > > > Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. > > > Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. > > > In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. > > > If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. > > > One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. > > > Ray > Phenopath Labs > Seattle WA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From koellingr <@t> comcast.net Tue Jan 31 08:14:54 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Jan 31 08:15:04 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested In-Reply-To: <4F27A825.2B7F.00C9.1@geisinger.edu> Message-ID: <1350046215.266731.1328019294499.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Fascinating... and that gets me back to my original ponderance. Why all the "false" histotechs? Are there people trying to sneak into flow cytometry who have never run a flow cytometer or clinical chemistry medtechs who have never sat in front of an analyzer or cytotechs who don't know an epithelial cell from a glandular cell. What is the reason that there seems to be so much trouble now with non-functioning or poorly functioning histotechs or outright imposters? Maybe there is an answer 2 or 3 levels globally deeper in health care and society than the simple question of "Can you cut a section at interview?" Ray Seattle ----- Original Message ----- From: "Angela Bitting" To: "Thomas Jasper" , "Kim Donadio" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 31, 2012 5:36:53 AM Subject: Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested I've had a temp, who we interviewed over the phone, come in and sit down at a microtome and create the most horrendous slides I've ever seen. He lasted a week and we sent him back from whence he came. I don't think he was EVER a Histotech or if he was it was many, many moons ago. Point is.....he snowed us all during the interview. Just thought I'd throw that out there. >>> Kim Donadio 1/30/2012 10:01 PM >>> Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) I don't think it should be made a big deal. You take a drivers test to drive. Peoples lives are on the line in each case. Does that a lone mean I don't hire them. Probaly not. I just need to know how much personal investment of my time I am going to need to give ...... Runs for her pillow of dreams :). Nite nite Kim Sent from my iPhone On Jan 28, 2012, at 4:25 PM, "Thomas Jasper" wrote: > Ray, > > Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. > > Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. > > Kind regards, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, OR 97701 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net > Sent: Saturday, January 28, 2012 10:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > > > > > > > > Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. > > > While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. > > > Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. > > > Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. > > > Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. > > > Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. > > > In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. > > > If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. > > > One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. > > > Ray > Phenopath Labs > Seattle WA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 31 08:28:02 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 31 08:28:11 2012 Subject: [Histonet] Coverslipping for excellent optical quality In-Reply-To: Message-ID: <1328020082.51459.YahooMailClassic@web162103.mail.bf1.yahoo.com> Jonathan: You have brought up some concerns that have been "into focus" since the mid XIX century, when the objectives manufacturers started to improve the quality of objectives. Let me go step by step: 1- you already claim that you have mastered the K?hler's illumination whose?purpose is limited to obtaining maximum illumination limited only in the area covered by each objective. This illumination is of paramount importance for observation but fundamentally for?photomicrography. 2- the?next problem tackled by the manufacturers (specially Zeiss and Leitz) was the development of objectives corrected in a way that allowed minimal color distortion and that is how the fluorite and apochromatic objectives appeared in the market. Now is where you "thickness" issue came to be of concern: 3- the space between the stained tissue and the frontal lens of the objective was calculated by each manufacturer because, in early models, the focal distance of the whole assembly (objective + ocular) was specific: 160mm for Zeiss, Bausch&Lomb and Spencer (American Optical) and 170mm for Leitz. 4- besides the required optical thickness the recommendation was always to have the least amount of mounting medium and glass coverslip between the tissue and the frontal lens of the objective and two fundamental types of coverslips were developed; the so called "No1" which was/is thinner; and the "No2" which thicker. For photomicrography you would never use a "No2" coverslip. 5- to determine the thickness of the coverslips you take a given number (lets say 10) and use a mechanics micrometer to determine the thickness of the selected group and divide by 10 (or whatever amount of coverslips you used for the measurement). 6- the amount of mounting medium will vary but you have to make sure the the coverslip is "pressed down" enough as to make sure that the contact is intimate and minimal. 7- as you can see,these are too many "approximations" so the objectives manufacturers came with an optic-mechanical solution and developed the "correction collar" which is a?group of middle lenses mounted on a moving thread that allows those lenses to focus the image to compensate for the thickness of the space mounting medium + coverlip to obtain a perfect compensated focus. 8- those collars were provided with "high-dry" objectives (usually of magnification 40:1 and higher with NA 0.95 and higher) and only for apochromatic objectives. Baush&Lomb, Zeiss and Leitz (among others) developed?such objectives with correction collars. 9- but all of that became of secondary importance when in the late 1950's all manufacturers developed objectives with focus at infinity (the are marked as ?) and those limitations were eliminated. ? My final consideration is that for routine work?you do not need neither K?hler's illumination or have to worry about the thickness of the space between the specimen and the frontal lens of the objective. Some pathologists bemoan about the use of film to cover the sections because they think that the "uneven" thickness of the film will hamper?photomicrography quality, but that is not the case. My recommendation to you is that you should not worry that much. If you intend to take photomicrographies during your work, just use K?hler's illumination to limit the field of vision, use the condenser diaphragm to control the intensity of light, use coverslips "No1" making sure that there is no excess mounting medium. Try to use at least fluorite objectives and take your photos. They will come very good. Ren? J.? ? --- On Tue, 1/31/12, Jonathan Cremer wrote: From: Jonathan Cremer Subject: [Histonet] Coverslipping for excellent optical quality To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, January 31, 2012, 5:56 AM Dear Histonetters, I am currently reading up on proper use and maintenance of microscopes, and inevitably stumbled across K?hler's illumination. No one ever explained this to me, although I have a 'microscopy protocol' that comes close to properly explaining the correct setup. So now that I know how to properly use a microscope fitted with such a system, there is still the issue of the coverslip/mounting medium which are considered the 'first lens'. I understand why there is a narrow tolerance for coverslip/mounting medium thickness to achieve optimal performance, but how do you control that thickness? Currently, when I mount a slide, I keep it wetted with xylene, put a sufficient amount of mounting medium on the coverslip and mount in the usual 'rolling' motion. I then push on the coverslip to remove trapped bubbles (if any) and be sure to spread the glue under the entire coverslip. However, how can one be sure about the thickness of the mounting medium layer? Using more/less glue and applying more/less pressure to the coverslip must surely influence this? Especially since we're talking microns. Does anybody have any insights or tips, or I am just nitpicking and trying to achieve an impossible result? :) Regards, Jonathan --- Jonathan Cremer Laboratory Technician Gastro-enterologie KU Leuven, Belgium _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 31 08:34:06 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 31 08:34:14 2012 Subject: [Histonet] Processing adipose tissue In-Reply-To: Message-ID: <1328020446.25334.YahooMailClassic@web162101.mail.bf1.yahoo.com> I agree that 21 hours is too much, but using isopropyl alcohol does not a "clearing" step because isopropyl alcohol will dissolve paraffin wax at 50?C and above. This is the method I have published else were with the intermediate step of mineral oil (which is paraffin of low molecular weight) to reduce the gradient and protect the tissue structure. The Peloris processor routinely uses this sequence (isopropyl ? paraffin wax) and is widely used in Australia. The problem resides in the dehydration time. I will also suggest an intermediate step of isopropyl alcohol+paraffin wax 1:1 between the last alcohol and the first paraffin wax bath. Ren? J. --- On Mon, 1/30/12, Jerry Ricks wrote: From: Jerry Ricks Subject: RE: [Histonet] Processing adipose tissue To: histonet@lists.utsouthwestern.edu Date: Monday, January 30, 2012, 7:15 PM Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to remove the IPA." I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes. http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&ved=0CGMQFjAJ&url=http%3A%2F%2Fwww.ebsciences.com%2Fpapers%2FMineral%2520oil%2520as%2520xylene%2520substitute.pdf&ei=ny4nT73bM8axiQLk3dmQAQ&usg=AFQjCNFw894if_bFAYgv6Uurw2MkO3Th5A Jerry > Date: Mon, 30 Jan 2012 12:20:24 -0600 > From: David.Burk@pbrc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing adipose tissue > > Esteemed experts, > >? > > We have many clients who want to process mouse and human adipose tissue > and are having some quality issues in the resultant slides.? > > We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our > automated processor (Excelsior) as follows: > > Tissue fixed for ~24 hrs in 10% NBF > > 70% Isopropyl alcohol (IPA) for 3 hrs > > 90% IPA, 3hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > >? > > Embed and section at 5 um prior to H&E.? An example of what the sections > look like can be found here http://imgur.com/7RTGR .? > > We also ran a sample on a "traditional overnight" EtOH/Xylene processor > (not at our facility) to compare results.? That image is here: > http://imgur.com/GjJPg .? > > What is obvious is that the membranes in the IPA processed tissue seem > to "flap over" and don't look as crisp as the Xylene processed tissue.? > > We did notice structural defects in both samples (not shown) typically > toward the middle of the specimens. > >? > > Does anyone know what is causing our IPA processed fat to have these > "wide membrane" artifacts?? > > We are going to repeat the process with an additional 30 minutes per > step and raise the temperature of the steps to ~ 35 C.? > > We are also going to cut the blocks at 2-3 um to see if it can reduce > the appearance of the membranes.? > >? > > Thanks very much for any advice you may have for us.? We are pretty > locked in to using xylene-free processing methodology if at all possible > but will entertain any suggestions you may have.? > > If I can provide any further details about what we are doing on our end, > please let me know and I'll be happy to provide them. > >? > > Best, > > David Burk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Burk <@t> pbrc.edu Tue Jan 31 08:51:36 2012 From: David.Burk <@t> pbrc.edu (David Burk) Date: Tue Jan 31 08:51:44 2012 Subject: [Histonet] Processing adipose tissue In-Reply-To: <1328020446.25334.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1328020446.25334.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: <281CF76A690FA74783D6EF0EC4EF243C2216D1@pbrcas30.pbrc.edu> Ren?, Thanks very much for your input on this matter. I also want to go ahead and thank the other two (Jerry and Karen) who have chimed in on my question. Ren?, could you please elaborate on "the problem resides in the dehydration time"? Am I over doing it with 21 hours of IPA? As for the suggestion about introducing an intermediate step, I am going to find out if that is doable on our Excelsior and, if so, replace the last (9th) IPA step with either an IPA/Paraffin mix or perhaps a mineral oil mix. If anyone else has further suggestions as to help us get nice crisp membrane structure from our adipose sections I will be very happy to hear them. Also, if you need additional example images from our sectioned material, I can provide them easily. Best, David B. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 31, 2012 8:34 AM To: histonet@lists.utsouthwestern.edu; Jerry Ricks Subject: RE: [Histonet] Processing adipose tissue I agree that 21 hours is too much, but using isopropyl alcohol does not a "clearing" step because isopropyl alcohol will dissolve paraffin wax at 50?C and above. This is the method I have published else were with the intermediate step of mineral oil (which is paraffin of low molecular weight) to reduce the gradient and protect the tissue structure. The Peloris processor routinely uses this sequence (isopropyl ? paraffin wax) and is widely used in Australia. The problem resides in the dehydration time. I will also suggest an intermediate step of isopropyl alcohol+paraffin wax 1:1 between the last alcohol and the first paraffin wax bath. Ren? J. --- On Mon, 1/30/12, Jerry Ricks wrote: From: Jerry Ricks Subject: RE: [Histonet] Processing adipose tissue To: histonet@lists.utsouthwestern.edu Date: Monday, January 30, 2012, 7:15 PM Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to remove the IPA." I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes. http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&ved=0CGMQFjAJ&url=http%3A%2F%2Fwww.ebsciences.com%2Fpapers%2FMineral%2520oil%2520as%2520xylene%2520substitute.pdf&ei=ny4nT73bM8axiQLk3dmQAQ&usg=AFQjCNFw894if_bFAYgv6Uurw2MkO3Th5A Jerry > Date: Mon, 30 Jan 2012 12:20:24 -0600 > From: David.Burk@pbrc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing adipose tissue > > Esteemed experts, > >? > > We have many clients who want to process mouse and human adipose > tissue and are having some quality issues in the resultant slides. > > We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on > our automated processor (Excelsior) as follows: > > Tissue fixed for ~24 hrs in 10% NBF > > 70% Isopropyl alcohol (IPA) for 3 hrs > > 90% IPA, 3hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > >? > > Embed and section at 5 um prior to H&E.? An example of what the > sections look like can be found here http://imgur.com/7RTGR . > > We also ran a sample on a "traditional overnight" EtOH/Xylene > processor (not at our facility) to compare results.? That image is here: > http://imgur.com/GjJPg . > > What is obvious is that the membranes in the IPA processed tissue seem > to "flap over" and don't look as crisp as the Xylene processed tissue. > > We did notice structural defects in both samples (not shown) typically > toward the middle of the specimens. > >? > > Does anyone know what is causing our IPA processed fat to have these > "wide membrane" artifacts? > > We are going to repeat the process with an additional 30 minutes per > step and raise the temperature of the steps to ~ 35 C. > > We are also going to cut the blocks at 2-3 um to see if it can reduce > the appearance of the membranes. > >? > > Thanks very much for any advice you may have for us.? We are pretty > locked in to using xylene-free processing methodology if at all > possible but will entertain any suggestions you may have. > > If I can provide any further details about what we are doing on our > end, please let me know and I'll be happy to provide them. > >? > > Best, > > David Burk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 31 08:57:11 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 31 08:57:21 2012 Subject: [Histonet] Processing adipose tissue In-Reply-To: <281CF76A690FA74783D6EF0EC4EF243C2216D1@pbrcas30.pbrc.edu> Message-ID: <1328021831.71784.YahooMailClassic@web162101.mail.bf1.yahoo.com> David: 21 hours dehydration is too much, but the main problem resides in the "abrupt"?transition from alcohol to paraffin wax, especially with fat tissue. Under separate cover I am sending you the article I referred to. Ren? J. --- On Tue, 1/31/12, David Burk wrote: From: David Burk Subject: RE: [Histonet] Processing adipose tissue To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 31, 2012, 9:51 AM Ren?, Thanks very much for your input on this matter.? I also want to go ahead and thank the other two (Jerry and Karen) who have chimed in on my question.? Ren?, could you please elaborate on "the problem resides in the dehydration time"?? Am I over doing it with 21 hours of IPA?? As for the suggestion about introducing an intermediate step, I am going to find out if that is doable on our Excelsior and, if so, replace the last (9th) IPA step with either an IPA/Paraffin mix or perhaps a mineral oil mix. If anyone else has further suggestions as to help us get nice crisp membrane structure from our adipose sections I will be very happy to hear them.? Also, if you need additional example images from our sectioned material, I can provide them easily. Best, David B. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 31, 2012 8:34 AM To: histonet@lists.utsouthwestern.edu; Jerry Ricks Subject: RE: [Histonet] Processing adipose tissue I agree that 21 hours is too much, but using isopropyl alcohol does not a "clearing" step because isopropyl alcohol will dissolve paraffin wax at 50?C and above. This is the method I have published else were with the intermediate step of mineral oil (which is paraffin of low molecular weight) to reduce the gradient and protect the tissue structure. The Peloris processor routinely uses this sequence (isopropyl ? paraffin wax) and is widely used in Australia. The problem resides in the dehydration time. I will also suggest an intermediate step of isopropyl alcohol+paraffin wax 1:1 between the last alcohol and the first paraffin wax bath. Ren? J. --- On Mon, 1/30/12, Jerry Ricks wrote: From: Jerry Ricks Subject: RE: [Histonet] Processing adipose tissue To: histonet@lists.utsouthwestern.edu Date: Monday, January 30, 2012, 7:15 PM Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to remove the IPA." I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes. http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&ved=0CGMQFjAJ&url=http%3A%2F%2Fwww.ebsciences.com%2Fpapers%2FMineral%2520oil%2520as%2520xylene%2520substitute.pdf&ei=ny4nT73bM8axiQLk3dmQAQ&usg=AFQjCNFw894if_bFAYgv6Uurw2MkO3Th5A Jerry > Date: Mon, 30 Jan 2012 12:20:24 -0600 > From: David.Burk@pbrc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing adipose tissue > > Esteemed experts, > >? > > We have many clients who want to process mouse and human adipose > tissue and are having some quality issues in the resultant slides. > > We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on > our automated processor (Excelsior) as follows: > > Tissue fixed for ~24 hrs in 10% NBF > > 70% Isopropyl alcohol (IPA) for 3 hrs > > 90% IPA, 3hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > >? > > Embed and section at 5 um prior to H&E.? An example of what the > sections look like can be found here http://imgur.com/7RTGR . > > We also ran a sample on a "traditional overnight" EtOH/Xylene > processor (not at our facility) to compare results.? That image is here: > http://imgur.com/GjJPg . > > What is obvious is that the membranes in the IPA processed tissue seem > to "flap over" and don't look as crisp as the Xylene processed tissue. > > We did notice structural defects in both samples (not shown) typically > toward the middle of the specimens. > >? > > Does anyone know what is causing our IPA processed fat to have these > "wide membrane" artifacts? > > We are going to repeat the process with an additional 30 minutes per > step and raise the temperature of the steps to ~ 35 C. > > We are also going to cut the blocks at 2-3 um to see if it can reduce > the appearance of the membranes. > >? > > Thanks very much for any advice you may have for us.? We are pretty > locked in to using xylene-free processing methodology if at all > possible but will entertain any suggestions you may have. > > If I can provide any further details about what we are doing on our > end, please let me know and I'll be happy to provide them. > >? > > Best, > > David Burk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steven <@t> prometheushealthcare.com Tue Jan 31 09:02:52 2012 From: steven <@t> prometheushealthcare.com (Steven-Prometheus) Date: Tue Jan 31 09:03:06 2012 Subject: [Histonet] Several Open Histotech Positions in NY area Message-ID: <002e01cce029$67d2a200$3777e600$@prometheushealthcare.com> Several open Histotech positions for top hospitals and labs in the New York area looking to be filled immediately. 2nd and 3rd shifts available. Extremely competitive salaries and other benefits at each location. Please contact us today if you know someone who would be interested. Thanks so much for your help! (NY certification required) Steven Wagner National Account Manager Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 steven@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From wdesalvo.cac <@t> hotmail.com Tue Jan 31 09:18:17 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Jan 31 09:18:26 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested In-Reply-To: <1350046215.266731.1328019294499.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <4F27A825.2B7F.00C9.1@geisinger.edu>, <1350046215.266731.1328019294499.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: I have been following the string and I see the issue from a different perspective. I have always found it difficult to find qualified and registered techs and have been training science degreed individuals as bench techs for several years. I think the issue is identifying the proper individual to add to your team. Technical skill is important, but attitude, aptitude, desire and how they will ultimately fit into and what they will add to the team are by far more important. The question that may be better to ask is "How do you cut a section and why do you cut a section?". Just being able to "cut" a section is not necessarily going to give you enough information to decide if the individual really fits into the team, no better yet, fit into your culture. Hiring an individual that does not fit into your lab/company and can fully support and promote the mission, vision and goals, will not help you. Why this is now becoming more of an issue may be due to the fact that with the shortage of techs in Histology, the situation exists where we have close to full employment of all registered and qualified techs. When that situation occurs, there will be more opportunities for less skill qualified individuals to obtain employment. I would not go so far as to call less skillful techs "imposters" or "false", but maybe book smart and not skill smart. Hiring an individual to perform to the quality and productivity standards of the lab requires significant investment of time to train. Now the catch 22 starts, you must invest time to properly add to your team and you do not believe you have time to invest. Once you bring a new member into your team, there is a cycle (training, functionality and competency) that must take place. You must identify were an individual fits into that cycle and how much time will be required to move them to competency. I have seen both skill qualified and non-skill qualified candidates take the same time to reach functionality. Again, I believe attitude is more key than technical skill. Without the proper attitude you will not quickly reach functionality and functionality allows you to gain time. Competency is the end goal, but functionality is the first critical step. To properly move through the cycle you must have a detailed, documented and functional training process. This whole discussion speaks to me that there is a lack of written and documented standardized training (it works for MT's) and to develop standardized training you must have standardized procedures and techniques (you knew I would work this into the discussion). The degree of difficulty of an individual to meet the quality and productivity requirements of the lab depends on the amount of standardization in the lab. Couple a standardized process with attitude and desire and you can quickly develop a new hire into a fully functioning member of the team. I believe that is the real goal and that will help Histotechnology progress. Is attitude, aptitude and desire really exposed when you ask a candidate "can you cut a section"? William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 31 Jan 2012 14:14:54 +0000 > From: koellingr@comcast.net > To: akbitting@geisinger.edu > Subject: Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > CC: histonet@lists.utsouthwestern.edu > > Fascinating... and that gets me back to my original ponderance. Why all the "false" histotechs? Are there people trying to sneak into flow cytometry who have never run a flow cytometer or clinical chemistry medtechs who have never sat in front of an analyzer or cytotechs who don't know an epithelial cell from a glandular cell. What is the reason that there seems to be so much trouble now with non-functioning or poorly functioning histotechs or outright imposters? Maybe there is an answer 2 or 3 levels globally deeper in health care and society than the simple question of "Can you cut a section at interview?" > Ray > Seattle > > ----- Original Message ----- > From: "Angela Bitting" > To: "Thomas Jasper" , "Kim Donadio" > Cc: histonet@lists.utsouthwestern.edu > Sent: Tuesday, January 31, 2012 5:36:53 AM > Subject: Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > I've had a temp, who we interviewed over the phone, come in and sit down at a microtome and create the most horrendous slides I've ever seen. He lasted a week and we sent him back from whence he came. I don't think he was EVER a Histotech or if he was it was many, many moons ago. Point is.....he snowed us all during the interview. Just thought I'd throw that out there. > > >>> Kim Donadio 1/30/2012 10:01 PM >>> > Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) > > I don't think it should be made a big deal. You take a drivers test to drive. Peoples lives are on the line in each case. > > Does that a lone mean I don't hire them. Probaly not. I just need to know how much personal investment of my time I am going to need to give ...... > > Runs for her pillow of dreams :). Nite nite > Kim > > Sent from my iPhone > > On Jan 28, 2012, at 4:25 PM, "Thomas Jasper" wrote: > > > Ray, > > > > Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. > > > > Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. > > > > Kind regards, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, OR 97701 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net > > Sent: Saturday, January 28, 2012 10:23 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > > > > > > > > > > > > > > > > > Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. > > > > > > While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. > > > > > > Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. > > > > > > Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. > > > > > > Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. > > > > > > Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. > > > > > > In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. > > > > > > If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. > > > > > > One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. > > > > > > Ray > > Phenopath Labs > > Seattle WA > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Tue Jan 31 09:28:11 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Jan 31 09:28:30 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested In-Reply-To: Message-ID: <363025691.269313.1328023691615.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Great and fantastically said and to answer your last question in my opinion "No!!!!!!!!!!". My original point when this started. Ray Seattle ----- Original Message ----- From: "WILLIAM DESALVO" To: koellingr@comcast.net, akbitting@geisinger.edu Cc: "histonet" Sent: Tuesday, January 31, 2012 7:18:17 AM Subject: RE: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested I have been following the string and I see the issue from a different perspective. I have always found it difficult to find qualified and registered techs and have been training science degreed individuals as bench techs for several years. I think the issue is identifying the proper individual to add to your team. Technical skill is important, but attitude, aptitude, desire and how they will ultimately fit into and what they will add to the team are by far more important. The question that may be better to ask is "How do you cut a section and why do you cut a section?". Just being able to "cut" a section is not necessarily going to give you enough information to decide if the individual really fits into the team, no better yet, fit into your culture. Hiring an individual that does not fit into your lab/company and can fully support and promote the mission, vision and goals, will not help you. Why this is now becoming more of an issue may be due to the fact that with the shortage of techs in Histology, the situation exists where we have close to full employment of all registered and qualified techs. When that situation occurs, there will be more opportunities for less skill qualified individuals to obtain employment. I would not go so far as to call less skillful techs "imposters" or "false", but maybe book smart and not skill smart. Hiring an individual to perform to the quality and productivity standards of the lab requires significant investment of time to train. Now the catch 22 starts, you must invest time to properly add to your team and you do not believe you have time to invest. Once you bring a new member into your team, there is a cycle (training, functionality and competency) that must take place. You must identify were an individual fits into that cycle and how much time will be required to move them to competency. I have seen both skill qualified and non-skill qualified candidates take the same time to reach functionality. Again, I believe attitude is more key than technical skill. Without the proper attitude you will not quickly reach functionality and functionality allows you to gain time. Competency is the end goal, but functionality is the first critical step. To properly move through the cycle you must have a detailed, documented and functional training process. This whole discussion speaks to me that there is a lack of written and documented standardized training (it works for MT's) and to develop standardized training you must have standardized procedures and techniques (you knew I would work this into the discussion). The degree of difficulty of an individual to meet the quality and productivity requirements of the lab depends on the amount of standardization in the lab. Couple a standardized process with attitude and desire and you can quickly develop a new hire into a fully functioning member of the team. I believe that is the real goal and that will help Histotechnology progress. Is attitude, aptitude and desire really exposed when you ask a candidate "can you cut a section"? William DeSalvo, B. S., HTL(ASCP) > Date: Tue, 31 Jan 2012 14:14:54 +0000 > From: koellingr@comcast.net > To: akbitting@geisinger.edu > Subject: Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > CC: histonet@lists.utsouthwestern.edu > > Fascinating... and that gets me back to my original ponderance. Why all the "false" histotechs? Are there people trying to sneak into flow cytometry who have never run a flow cytometer or clinical chemistry medtechs who have never sat in front of an analyzer or cytotechs who don't know an epithelial cell from a glandular cell. What is the reason that there seems to be so much trouble now with non-functioning or poorly functioning histotechs or outright imposters? Maybe there is an answer 2 or 3 levels globally deeper in health care and society than the simple question of "Can you cut a section at interview?" > Ray > Seattle > > ----- Original Message ----- > From: "Angela Bitting" > To: "Thomas Jasper" , "Kim Donadio" > Cc: histonet@lists.utsouthwestern.edu > Sent: Tuesday, January 31, 2012 5:36:53 AM > Subject: Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > I've had a temp, who we interviewed over the phone, come in and sit down at a microtome and create the most horrendous slides I've ever seen. He lasted a week and we sent him back from whence he came. I don't think he was EVER a Histotech or if he was it was many, many moons ago. Point is.....he snowed us all during the interview. Just thought I'd throw that out there. > > >>> Kim Donadio 1/30/2012 10:01 PM >>> > Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) > > I don't think it should be made a big deal. You take a drivers test to drive. Peoples lives are on the line in each case. > > Does that a lone mean I don't hire them. Probaly not. I just need to know how much personal investment of my time I am going to need to give ...... > > Runs for her pillow of dreams :). Nite nite > Kim > > Sent from my iPhone > > On Jan 28, 2012, at 4:25 PM, "Thomas Jasper" wrote: > > > Ray, > > > > Took the time to read your post. You make excellent points. Getting at the gist of your "wannabee" comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. > > > > Seems to me this isn't Leonardo di Caprio and "Catch Me If You Can". In the end you are right about finding ways to determine if an applicant is "legit". I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. > > > > Kind regards, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, OR 97701 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net > > Sent: Saturday, January 28, 2012 10:23 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested > > > > > > > > > > > > > > > > > > Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who "cut out" on an interview. > > > > > > While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine "can cut (section) on rotary microtome" check box on application the same as you do for a "current address" or "reference contact" check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the "histology community" well known). If I call "x" who I've known for years about an applicant "y" who is applying and worked with "x" and am told "Oh! "y" worked for us for last 4 years. He/she along with "z" and "zz" were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her". Having him/her sit down to now cut 10 blocks to see "if they can cut" as a routine question accomplishes WHAT?" If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of "we need a recut, what would you do for this block" will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking "interpret this section" will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the "symphony" of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box "can cut" I think is just a waste of time and resources unless a particular circumstance warrants it. > > > > > > Someone asked "would you hire a secretary without a wpm typing test". Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant worked for last 10 years and "she's an immaculate and fast typist beyond anything we've ever had and so sorry she had to move", I'd rather then concentrate on more esoteric matrices than wpm. If he/she was a secretary 25 years ago and has been a house-husband or house-wife for 25 years and starting back now or if someone walks in off the street to apply then beyond any doubt; they take a typing test. > > > > > > Someone pointed out that all musicians play their instrument in application to test for the orchestra. Of course but for a completely different reason. You could give an "oral test" to 1,000 musicians of which 999 would know how to transpose 3 pitches up by 7 semi-tones or define a diatonic scale or identify the composer if listening to an excerpt from the Overture-Midsummers Night Dream. That's not what the interviewee is looking for. They are looking for the ONE in 1,000 who has the exact pitch, timbre, affannato, vibrato, arioso and legato from their specific instrument that only that particular person's instrument and ability possesses. Only a finely trained ear (the conductor) has that God-given ability of relative/perfect pitch or undefinable gift to identify that one instrument and one ability to fit into the total music experience. And there is only one way to find out; have him or her play. Totally different scenario than in a histology lab unless the object is to see how well the speed and noise of one person's cutting blends in with the symphony of 75 other microtomes being used in the lab at the same time. > > > > > > Then you start to ponder, as did a fine mind out there who understood the butterfly comment, if a current 30-year superstar of histology walked into a lab looking for a histology job, would they take a cutting ( sectioning?) test? If Yo-Yo Ma or James Galway or Itzhak Perlman or John Cerminaro had ever walked in to "test" for an orchestral position, surely they wouldn't be tested just to see if "can they play" a cello or flute or violin or French Horn or even how well they play on that particular day in that particular environment. > > > > > > Maybe what I'm mis-understanding is that apparently there are A LOT of histology wannabees, walking in off the street trying to "sneak into histology"? and if so that seems like there should be some manner of response to that situation although not sure what it is. But something short of sitting down to cut and have them slice a finger. And if accredited histology schools are putting out graduated students with HT certifications, and have never cut a block or only 3 blocks or trained to routinely cut thick and thin, then that seems a matter for the school, NSH, NACCLS, ASCP, CAP, state agencies, etc and not the histonet. > > > > > > In the end, I think there are potentially far better ways (and there have been numerous great suggestions already) to ascertain information about an applicant than a routine (check-accomplished cutting 10 blocks) check-off box although depending on the situation, I'm not at all against cutting blocks at application if warranted. > > > > > > If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview. > > > > > > One day about a year ago, I sat down and did some fairly accurate (I think) estimation of "how many blocks have I cut in 45 years in pathology" . Came up with a number a bit above 1,100,000 blocks (paraffin, frozen OCT, glycol methacrylate, EPON). So if I come looking for a bench histology job, hope I can skip the routine, required "can section?" check box. Would rather spend the time talking about the greatest sports franchise in the history of all sports; The St. Louis Baseball Cardinals. Summer of 1967 cut my first paraffin block while on high school summer break (after a few weeks learning to hone my steel knife with a Belgian stone and sharpening/stropping with a barber razor strop). And in summer of 1967 I also watched an unhitable Bob Gibson lead the Cards to yet another World Series. > > > > > > Ray > > Phenopath Labs > > Seattle WA > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Jan 31 09:30:55 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jan 31 09:31:02 2012 Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested In-Reply-To: References: <4F27A825.2B7F.00C9.1@geisinger.edu> <1350046215.266731.1328019294499.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: For myself, I can say this: I can give you the best sections you've ever seen on my cryostat. But when I tried to use a cryostat that had disposable blades (in which you have to pick the section up from a completely different angle), I was terrible at first. I think you really need to get a technique down. It takes a few slides to get used to a microtome, at least a frozen one. Maybe with a paraffin microtome it's different because getting the section on the slide isn't about the knife angle and the slide. Either way, if I had to do sectioning on an interview, I would be so nervous, I'd probably do terribly. That said, thank god I have a job (for now!). Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From Allison_Scott <@t> hchd.tmc.edu Tue Jan 31 09:50:17 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Jan 31 09:50:24 2012 Subject: [Histonet] Rusting of paraffin reservoir Message-ID: Hello to all I histoland. I just discovered that my first paraffin pot has a hole in it due to rust. And of course all of the paraffin overtime had leaked out. Does anyone have a idea what may have contributed to this happening? I think that it had to do with some condensation. We have had this VIP-5 for about 8 years and never had this to occur. Any thoughts? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Heidi.Hawthorne <@t> onassignment.com Tue Jan 31 09:59:25 2012 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Tue Jan 31 09:59:37 2012 Subject: [Histonet] Job Opportunity - Northern CA Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B8326E36AB12E@oasslcexm01.oaifield.onasgn.com> Hello! We have an immediate need for a Histotechnician for a state-of-the-art Medical Center in Martinez, CA . Day/early morning shift available working in a hospital lab. Requirements: At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at: http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 From rsrichmond <@t> gmail.com Tue Jan 31 10:06:37 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Jan 31 10:06:45 2012 Subject: [Histonet] Re: Carbon 14 Message-ID: Jim Staruk asks: >>Does anyone have any experience with tissue injected with carbon 14? I have some questions about precautions and disposal.<< Natural carbon derived from atmospheric carbon (like the tissues of plants and animals) contains some natural carbon 14, formed in the upper atmosphere by cosmic-ray irradiation of nitrogen 14. So when you incinerate carbon 14 labeled material, you're adding some more carbon 14 to a smokestack that's already emitting some anyway. The isotope has a half-life of around 5,700 years, undergoing beta decay whose product is nitrogen 14. To put some numbers on that: when carbon 14 labeled glucose was used in clinical blood culture bottles, it was estimated that incinerating the bottles would roughly double a hospital incinerator's smokestack emissions of the isotope, an amount that was considered safe by regulators. As Ren? Buesa suggests, I'd certainly check with a radiation safety officer (if you can find one), but I think you can probably incinerate this material. I have no idea if you can landfill it or not. Bob Richmond Samurai Pathologist Knoxville TN From MHillmer <@t> dermwisconsin.com Tue Jan 31 10:16:05 2012 From: MHillmer <@t> dermwisconsin.com (Michael Hillmer) Date: Tue Jan 31 10:16:14 2012 Subject: [Histonet] Job Openings Message-ID: We have two openings with our company at this time. The first position is in our Oshkosh, WI clinic, working with our Mohs team. This is 1st shift position, full-time postion and relocation assistance is available. The 2nd position is in our state-of-the-art pathology lab along the shores of Lake Michigan in downtown Manitowoc. We are looking for an HT/HtL with previous leadership and training experience. This would be an excellent growth postion for somone looking to gain more management experience. Relocation assistance is available as well. Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 Fax: (920)663-9004 Cell: (920)860-6360 mhillmer@dermwisconsin.com . ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From TNMayer <@t> mdanderson.org Tue Jan 31 10:38:58 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Jan 31 10:39:06 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: References: Message-ID: Jerry, I agree with you somewhat. I have met techs that misrepresented themselves and said that they could cut or embed, and knew how to operate the instruments, but could not produce quality work. You are right when you said that it is different for clinical vs. research. I have almost always worked clinical, and noticed that when working with research techs, they had a difficult time adjusting to clinical with the time frames and quality. When training new hires, depending on the position I am hiring for, I expect to train in the new workflow that they have learned, the new instrument they use, not the basic skills. I only expect to do that with a student. Fresh techs are expected to know how to get a section, not cut the plastic on the block, embed skin, and set up the h&e stainer. I should only have to go over and orient them on "our procedure" not teach the skill. I have worked various part-time jobs over the years and the first thing I ask is 'how many microns do you cut at here'? While 3-4 is the standard, some labs want everything at 3, or some at 4. I know how to cut, but like you it takes about 2 weeks to get used to the new instrument. That's fine, but I don't expect to have to teach the tech how to embed a skin or cut a kidney biopsy. Not for an experienced tech, unless they have never encountered it. That has to be made known during the interview. Yes, a cutting test is good, I have seen registered techs not make it past probation (90 days) because they could not cut. It would have saved the company time and MONEY if a test could have been given. Asking if they can cut a kidney biopsy, or embed a skin would be good as well. I can't go back and get more epithelia or ask for another pass through the kidney. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 5 Date: Mon, 30 Jan 2012 11:13:11 -0800 From: Jerry Ricks Subject: [Histonet] Interviewing Histotechs... To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. Jerry Ricks Research Scientist University of Washington Department of Pathology histonet@lists.utsouthwestern.edu > Date: Sun, 29 Jan 2012 13:12:09 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: interview.... > > Ray Koelling asked me: > > >>If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview.<< > > I really have no idea how many slides. In a normal year I sign out > about 3,000 histology cases (remember I don't work full time) > averaging maybe 3 slides per case. > > Generally I've gotten jobs, both private clients and agency clients, > by recommendation. A number of years ago I was interviewed by a > four-pathologist hospital group who handed me a tray of 20 slides with > the necessary historical information, and was told that this was a set > the group had collected, including very straightforward cases, cases > with serious diagnostic pitfalls, and some cases they'd never been > able to make a diagnosis on. They tried to make it a test of judgment > rather than simple diagnostic skill. Told to take as much time as I > needed. I guess I passed - by coincidence, the entire group chanced to > break up very quickly, and an entirely different team took over. > > Bob Richmond > Samurai Pathologist > Knoxville TN > ************************************ From tpheneger <@t> OSIP.com Tue Jan 31 11:06:13 2012 From: tpheneger <@t> OSIP.com (Pheneger, Tracy) Date: Tue Jan 31 11:06:19 2012 Subject: [Histonet] Processing adipose tissue In-Reply-To: References: <281CF76A690FA74783D6EF0EC4EF243C215F30@pbrcas30.pbrc.edu> Message-ID: <1F8A6EF2842BF5429D1487DA39FEE23F0A1E4F@bo-wexmb01.osip.com> Jerry- Ispropanol is completely miscible in Paraffin and doesn't need to be "cleared". David - I am also a proponent of Isopropanol processing of tissues, but I don't usually have really fatty tissues (mostly I deal with animal tissues). Unfortunately, I don't think that the Isopropanol methodology is infiltrative enough for really fatty tissues. I worry that if you increase your times and temps that you may damage the tissues. You may have to use an ETOH/Xylene - based system for these tissues. Please keep us informed on what you find out - there may be a paper or poster for you! Tracy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, January 30, 2012 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing adipose tissue Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any "clearing step to remove the IPA." I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes. http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&ved=0CGM QFjAJ&url=http%3A%2F%2Fwww.ebsciences.com%2Fpapers%2FMineral%2520oil%252 0as%2520xylene%2520substitute.pdf&ei=ny4nT73bM8axiQLk3dmQAQ&usg=AFQjCNFw 894if_bFAYgv6Uurw2MkO3Th5A Jerry > Date: Mon, 30 Jan 2012 12:20:24 -0600 > From: David.Burk@pbrc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing adipose tissue > > Esteemed experts, > > > > We have many clients who want to process mouse and human adipose tissue > and are having some quality issues in the resultant slides. > > We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our > automated processor (Excelsior) as follows: > > Tissue fixed for ~24 hrs in 10% NBF > > 70% Isopropyl alcohol (IPA) for 3 hrs > > 90% IPA, 3hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3 hrs > > 100% IPA, 3hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > Paraffin, 3 hrs > > > > Embed and section at 5 um prior to H&E. An example of what the sections > look like can be found here http://imgur.com/7RTGR . > > We also ran a sample on a "traditional overnight" EtOH/Xylene processor > (not at our facility) to compare results. That image is here: > http://imgur.com/GjJPg . > > What is obvious is that the membranes in the IPA processed tissue seem > to "flap over" and don't look as crisp as the Xylene processed tissue. > > We did notice structural defects in both samples (not shown) typically > toward the middle of the specimens. > > > > Does anyone know what is causing our IPA processed fat to have these > "wide membrane" artifacts? > > We are going to repeat the process with an additional 30 minutes per > step and raise the temperature of the steps to ~ 35 C. > > We are also going to cut the blocks at 2-3 um to see if it can reduce > the appearance of the membranes. > > > > Thanks very much for any advice you may have for us. We are pretty > locked in to using xylene-free processing methodology if at all possible > but will entertain any suggestions you may have. > > If I can provide any further details about what we are doing on our end, > please let me know and I'll be happy to provide them. > > > > Best, > > David Burk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 31 11:17:27 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 31 11:24:13 2012 Subject: [Histonet] Rusting of paraffin reservoir In-Reply-To: Message-ID: <1328030247.68140.YahooMailClassic@web162103.mail.bf1.yahoo.com> It is most likely due to, as you point out, to some condensation because paraffin wax will protect the metal preventing it to rust. Check for possible condensation. Ren? J. --- On Tue, 1/31/12, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Rusting of paraffin reservoir To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, January 31, 2012, 10:50 AM Hello to all I histoland.? I just discovered that my first paraffin pot has a? hole in it due to rust.? And of course all of the paraffin overtime had leaked out.? Does anyone have a idea what may have contributed to this happening?? I think that it had to do with some condensation.? We have had this VIP-5 for about 8 years and never had this to occur.? Any thoughts? Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Tue Jan 31 12:13:31 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Tue Jan 31 12:13:35 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: References: , Message-ID: Hi Toysha I think I'm just coming at it from "research mode" not clinical. Hands on Histotechnology is a core part of our work, but just part, and it is focused on animal models of cardiovascular disease. Depending on whether the researcher is a postdoc or an undergrad they will have more or fewer general lab skills including histo skills. I haven't met anyone yet who did not need some training for embedding of brachiocephalic arteries of mice. I doubt I would do well in a clinical lab. I've become accustomed to docs saying "wow that's beautiful can you teach me how to do that?" I gather in the clinical field it's more like "a monkey can learn how to section." Maybe a good monkey could but I doubt it could work up an IHC with a new antibody. Jerry > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 31 Jan 2012 10:38:58 -0600 > Subject: [Histonet] RE: Interviewing Histotechs... > > Jerry, > I agree with you somewhat. I have met techs that misrepresented themselves and said that they could cut or embed, and knew how to operate the instruments, but could not produce quality work. You are right when you said that it is different for clinical vs. research. I have almost always worked clinical, and noticed that when working with research techs, they had a difficult time adjusting to clinical with the time frames and quality. > When training new hires, depending on the position I am hiring for, I expect to train in the new workflow that they have learned, the new instrument they use, not the basic skills. I only expect to do that with a student. Fresh techs are expected to know how to get a section, not cut the plastic on the block, embed skin, and set up the h&e stainer. I should only have to go over and orient them on "our procedure" not teach the skill. > I have worked various part-time jobs over the years and the first thing I ask is 'how many microns do you cut at here'? While 3-4 is the standard, some labs want everything at 3, or some at 4. I know how to cut, but like you it takes about 2 weeks to get used to the new instrument. That's fine, but I don't expect to have to teach the tech how to embed a skin or cut a kidney biopsy. Not for an experienced tech, unless they have never encountered it. That has to be made known during the interview. > Yes, a cutting test is good, I have seen registered techs not make it past probation (90 days) because they could not cut. It would have saved the company time and MONEY if a test could have been given. Asking if they can cut a kidney biopsy, or embed a skin would be good as well. I can't go back and get more epithelia or ask for another pass through the kidney. > > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > > > Message: 5 > Date: Mon, 30 Jan 2012 11:13:11 -0800 > From: Jerry Ricks > Subject: [Histonet] Interviewing Histotechs... > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" > > Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. > > No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. > > If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > > > histonet@lists.utsouthwestern.edu > > Date: Sun, 29 Jan 2012 13:12:09 -0500 > > From: rsrichmond@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re: interview.... > > > > Ray Koelling asked me: > > > > >>If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview.<< > > > > I really have no idea how many slides. In a normal year I sign out > > about 3,000 histology cases (remember I don't work full time) > > averaging maybe 3 slides per case. > > > > Generally I've gotten jobs, both private clients and agency clients, > > by recommendation. A number of years ago I was interviewed by a > > four-pathologist hospital group who handed me a tray of 20 slides with > > the necessary historical information, and was told that this was a set > > the group had collected, including very straightforward cases, cases > > with serious diagnostic pitfalls, and some cases they'd never been > > able to make a diagnosis on. They tried to make it a test of judgment > > rather than simple diagnostic skill. Told to take as much time as I > > needed. I guess I passed - by coincidence, the entire group chanced to > > break up very quickly, and an entirely different team took over. > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > ************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Tue Jan 31 12:23:14 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Jan 31 12:23:40 2012 Subject: [Histonet] Louisiana Position Message-ID: <6F33D8418806044682A391273399860F0BA5CB1D@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From one_angel_secret <@t> yahoo.com Tue Jan 31 12:46:54 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jan 31 12:47:13 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: References: Message-ID: <782B4970-9895-4DFD-8AE9-563A1842E26A@yahoo.com> Your comment about a monkey hits a nerve. There is a misconception I think in our field that yes any monkey off the street can do our job. Well they can't. It takes a good amount of knowledge to understand tissues, stains, chemical reactions and yes you will need to have some amount of hand eye coordination Skill . It's the monkey theory that has histotechs jumping up and down into these quick almost no hands on programs so they can get a good paying job with not much invested I'm afraid. Yes, I'm an expert at sticking my foot in my mouth. But if we as a group don't recognize why we are even having this debate about testing techs on the most basic of functions. Then I worry about the future of our profession And even healthcare because by god if you want monkeys , think monkeys , you will get monkeys! And darn it. I can't run and hide from this one can I lol Have a great week! Kim D Sent from my iPhone On Jan 31, 2012, at 1:13 PM, Jerry Ricks wrote: > > > > > Hi Toysha > > I think I'm just coming at it from "research mode" not clinical. Hands on Histotechnology is a core part of our work, but just part, and it is focused on animal models of cardiovascular disease. Depending on whether the researcher is a postdoc or an undergrad they will have more or fewer general lab skills including histo skills. I haven't met anyone yet who did not need some training for embedding of brachiocephalic arteries of mice. > > I doubt I would do well in a clinical lab. I've become accustomed to docs saying "wow that's beautiful can you teach me how to do that?" I gather in the clinical field it's more like "a monkey can learn how to section." Maybe a good monkey could but I doubt it could work up an IHC with a new antibody. > > > Jerry > > >> From: TNMayer@mdanderson.org >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 31 Jan 2012 10:38:58 -0600 >> Subject: [Histonet] RE: Interviewing Histotechs... >> >> Jerry, >> I agree with you somewhat. I have met techs that misrepresented themselves and said that they could cut or embed, and knew how to operate the instruments, but could not produce quality work. You are right when you said that it is different for clinical vs. research. I have almost always worked clinical, and noticed that when working with research techs, they had a difficult time adjusting to clinical with the time frames and quality. >> When training new hires, depending on the position I am hiring for, I expect to train in the new workflow that they have learned, the new instrument they use, not the basic skills. I only expect to do that with a student. Fresh techs are expected to know how to get a section, not cut the plastic on the block, embed skin, and set up the h&e stainer. I should only have to go over and orient them on "our procedure" not teach the skill. >> I have worked various part-time jobs over the years and the first thing I ask is 'how many microns do you cut at here'? While 3-4 is the standard, some labs want everything at 3, or some at 4. I know how to cut, but like you it takes about 2 weeks to get used to the new instrument. That's fine, but I don't expect to have to teach the tech how to embed a skin or cut a kidney biopsy. Not for an experienced tech, unless they have never encountered it. That has to be made known during the interview. >> Yes, a cutting test is good, I have seen registered techs not make it past probation (90 days) because they could not cut. It would have saved the company time and MONEY if a test could have been given. Asking if they can cut a kidney biopsy, or embed a skin would be good as well. I can't go back and get more epithelia or ask for another pass through the kidney. >> >> >> >> Toysha N. Mayer, MBA, HT (ASCP) >> Instructor, Education Coordinator >> Program in Histotechnology >> School of Health Professions >> MD Anderson Cancer Center >> (713) 563-3481 >> tnmayer@mdanderson.org >> >> >> >> >> >> >> Message: 5 >> Date: Mon, 30 Jan 2012 11:13:11 -0800 >> From: Jerry Ricks >> Subject: [Histonet] Interviewing Histotechs... >> To: >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> >> I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" >> >> Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. >> >> No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. >> >> If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. >> >> Jerry Ricks >> Research Scientist >> University of Washington >> Department of Pathology >> >> >> >> histonet@lists.utsouthwestern.edu >>> Date: Sun, 29 Jan 2012 13:12:09 -0500 >>> From: rsrichmond@gmail.com >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Re: interview.... >>> >>> Ray Koelling asked me: >>> >>>>> If the Samurai Pathologist is out there reading still; any idea over your career, about how many glass slides have you viewed under a microscope since the first? Your replies are always top-notch, entertaining and informative. And hope with each new job you don't have to show someone you can pass a test of which slide shows normal liver and which slide shows cirrhotic liver in your interview.<< >>> >>> I really have no idea how many slides. In a normal year I sign out >>> about 3,000 histology cases (remember I don't work full time) >>> averaging maybe 3 slides per case. >>> >>> Generally I've gotten jobs, both private clients and agency clients, >>> by recommendation. A number of years ago I was interviewed by a >>> four-pathologist hospital group who handed me a tray of 20 slides with >>> the necessary historical information, and was told that this was a set >>> the group had collected, including very straightforward cases, cases >>> with serious diagnostic pitfalls, and some cases they'd never been >>> able to make a diagnosis on. They tried to make it a test of judgment >>> rather than simple diagnostic skill. Told to take as much time as I >>> needed. I guess I passed - by coincidence, the entire group chanced to >>> break up very quickly, and an entirely different team took over. >>> >>> Bob Richmond >>> Samurai Pathologist >>> Knoxville TN >>> >> ************************************ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Jan 31 13:03:27 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Jan 31 13:05:07 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: <782B4970-9895-4DFD-8AE9-563A1842E26A@yahoo.com> References: <782B4970-9895-4DFD-8AE9-563A1842E26A@yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3235D3D7B3@Mail2Node2.ad.uams.edu> Thank you and well said!!! Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, January 31, 2012 12:47 PM To: Jerry Ricks Cc: Subject: Re: [Histonet] RE: Interviewing Histotechs... Your comment about a monkey hits a nerve. There is a misconception I think in our field that yes any monkey off the street can do our job. Well they can't. It takes a good amount of knowledge to understand tissues, stains, chemical reactions and yes you will need to have some amount of hand eye coordination Skill . It's the monkey theory that has histotechs jumping up and down into these quick almost no hands on programs so they can get a good paying job with not much invested I'm afraid. Yes, I'm an expert at sticking my foot in my mouth. But if we as a group don't recognize why we are even having this debate about testing techs on the most basic of functions. Then I worry about the future of our profession And even healthcare because by god if you want monkeys , think monkeys , you will get monkeys! And darn it. I can't run and hide from this one can I lol Have a great week! Kim D Sent from my iPhone On Jan 31, 2012, at 1:13 PM, Jerry Ricks wrote: > > > > > Hi Toysha > > I think I'm just coming at it from "research mode" not clinical. Hands on Histotechnology is a core part of our work, but just part, and it is focused on animal models of cardiovascular disease. Depending on whether the researcher is a postdoc or an undergrad they will have more or fewer general lab skills including histo skills. I haven't met anyone yet who did not need some training for embedding of brachiocephalic arteries of mice. > > I doubt I would do well in a clinical lab. I've become accustomed to docs saying "wow that's beautiful can you teach me how to do that?" I gather in the clinical field it's more like "a monkey can learn how to section." Maybe a good monkey could but I doubt it could work up an IHC with a new antibody. > > > Jerry > > >> From: TNMayer@mdanderson.org >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 31 Jan 2012 10:38:58 -0600 >> Subject: [Histonet] RE: Interviewing Histotechs... >> >> Jerry, >> I agree with you somewhat. I have met techs that misrepresented themselves and said that they could cut or embed, and knew how to operate the instruments, but could not produce quality work. You are right when you said that it is different for clinical vs. research. I have almost always worked clinical, and noticed that when working with research techs, they had a difficult time adjusting to clinical with the time frames and quality. >> When training new hires, depending on the position I am hiring for, I expect to train in the new workflow that they have learned, the new instrument they use, not the basic skills. I only expect to do that with a student. Fresh techs are expected to know how to get a section, not cut the plastic on the block, embed skin, and set up the h&e stainer. I should only have to go over and orient them on "our procedure" not teach the skill. >> I have worked various part-time jobs over the years and the first thing I ask is 'how many microns do you cut at here'? While 3-4 is the standard, some labs want everything at 3, or some at 4. I know how to cut, but like you it takes about 2 weeks to get used to the new instrument. That's fine, but I don't expect to have to teach the tech how to embed a skin or cut a kidney biopsy. Not for an experienced tech, unless they have never encountered it. That has to be made known during the interview. >> Yes, a cutting test is good, I have seen registered techs not make it past probation (90 days) because they could not cut. It would have saved the company time and MONEY if a test could have been given. Asking if they can cut a kidney biopsy, or embed a skin would be good as well. I can't go back and get more epithelia or ask for another pass through the kidney. >> >> >> >> Toysha N. Mayer, MBA, HT (ASCP) >> Instructor, Education Coordinator >> Program in Histotechnology >> School of Health Professions >> MD Anderson Cancer Center >> (713) 563-3481 >> tnmayer@mdanderson.org >> >> >> >> >> >> >> Message: 5 >> Date: Mon, 30 Jan 2012 11:13:11 -0800 >> From: Jerry Ricks >> Subject: [Histonet] Interviewing Histotechs... >> To: >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> >> I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" >> >> Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. >> >> No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. >> >> If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. >> >> Jerry Ricks >> Research Scientist >> University of Washington >> Department of Pathology >> >> >> >> histonet@lists.utsouthwestern.edu >>> Date: Sun, 29 Jan 2012 13:12:09 -0500 >>> From: rsrichmond@gmail.com >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Re: interview.... >>> >>> Ray Koelling asked me: >>> >>>>> If the Samurai Pathologist is out there reading still; any idea >>>>> over your career, about how many glass slides have you viewed >>>>> under a microscope since the first? Your replies are always >>>>> top-notch, entertaining and informative. And hope with each new >>>>> job you don't have to show someone you can pass a test of which >>>>> slide shows normal liver and which slide shows cirrhotic liver in >>>>> your interview.<< >>> >>> I really have no idea how many slides. In a normal year I sign out >>> about 3,000 histology cases (remember I don't work full time) >>> averaging maybe 3 slides per case. >>> >>> Generally I've gotten jobs, both private clients and agency clients, >>> by recommendation. A number of years ago I was interviewed by a >>> four-pathologist hospital group who handed me a tray of 20 slides >>> with the necessary historical information, and was told that this >>> was a set the group had collected, including very straightforward >>> cases, cases with serious diagnostic pitfalls, and some cases they'd >>> never been able to make a diagnosis on. They tried to make it a test >>> of judgment rather than simple diagnostic skill. Told to take as >>> much time as I needed. I guess I passed - by coincidence, the entire >>> group chanced to break up very quickly, and an entirely different team took over. >>> >>> Bob Richmond >>> Samurai Pathologist >>> Knoxville TN >>> >> ************************************ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From PAMarcum <@t> uams.edu Tue Jan 31 13:06:15 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Jan 31 13:07:56 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: References: , Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3235D3D7C7@Mail2Node2.ad.uams.edu> I have done both clinical and research and that comment happens in both areas about teaching me how to do that kind of work from doctors. Before you say monkeys maybe you should try it full time in clinical and find out what you are talking about. After 47 years I am still learning both areas as nothing is static and egos will get you in trouble. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Tuesday, January 31, 2012 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Interviewing Histotechs... Hi Toysha I think I'm just coming at it from "research mode" not clinical. Hands on Histotechnology is a core part of our work, but just part, and it is focused on animal models of cardiovascular disease. Depending on whether the researcher is a postdoc or an undergrad they will have more or fewer general lab skills including histo skills. I haven't met anyone yet who did not need some training for embedding of brachiocephalic arteries of mice. I doubt I would do well in a clinical lab. I've become accustomed to docs saying "wow that's beautiful can you teach me how to do that?" I gather in the clinical field it's more like "a monkey can learn how to section." Maybe a good monkey could but I doubt it could work up an IHC with a new antibody. Jerry > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 31 Jan 2012 10:38:58 -0600 > Subject: [Histonet] RE: Interviewing Histotechs... > > Jerry, > I agree with you somewhat. I have met techs that misrepresented themselves and said that they could cut or embed, and knew how to operate the instruments, but could not produce quality work. You are right when you said that it is different for clinical vs. research. I have almost always worked clinical, and noticed that when working with research techs, they had a difficult time adjusting to clinical with the time frames and quality. > When training new hires, depending on the position I am hiring for, I expect to train in the new workflow that they have learned, the new instrument they use, not the basic skills. I only expect to do that with a student. Fresh techs are expected to know how to get a section, not cut the plastic on the block, embed skin, and set up the h&e stainer. I should only have to go over and orient them on "our procedure" not teach the skill. > I have worked various part-time jobs over the years and the first thing I ask is 'how many microns do you cut at here'? While 3-4 is the standard, some labs want everything at 3, or some at 4. I know how to cut, but like you it takes about 2 weeks to get used to the new instrument. That's fine, but I don't expect to have to teach the tech how to embed a skin or cut a kidney biopsy. Not for an experienced tech, unless they have never encountered it. That has to be made known during the interview. > Yes, a cutting test is good, I have seen registered techs not make it past probation (90 days) because they could not cut. It would have saved the company time and MONEY if a test could have been given. Asking if they can cut a kidney biopsy, or embed a skin would be good as well. I can't go back and get more epithelia or ask for another pass through the kidney. > > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > > > Message: 5 > Date: Mon, 30 Jan 2012 11:13:11 -0800 > From: Jerry Ricks > Subject: [Histonet] Interviewing Histotechs... > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" > > Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. > > No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. > > If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > > > histonet@lists.utsouthwestern.edu > > Date: Sun, 29 Jan 2012 13:12:09 -0500 > > From: rsrichmond@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re: interview.... > > > > Ray Koelling asked me: > > > > >>If the Samurai Pathologist is out there reading still; any idea > > >>over your career, about how many glass slides have you viewed > > >>under a microscope since the first? Your replies are always > > >>top-notch, entertaining and informative. And hope with each new > > >>job you don't have to show someone you can pass a test of which > > >>slide shows normal liver and which slide shows cirrhotic liver in > > >>your interview.<< > > > > I really have no idea how many slides. In a normal year I sign out > > about 3,000 histology cases (remember I don't work full time) > > averaging maybe 3 slides per case. > > > > Generally I've gotten jobs, both private clients and agency clients, > > by recommendation. A number of years ago I was interviewed by a > > four-pathologist hospital group who handed me a tray of 20 slides > > with the necessary historical information, and was told that this > > was a set the group had collected, including very straightforward > > cases, cases with serious diagnostic pitfalls, and some cases they'd > > never been able to make a diagnosis on. They tried to make it a test > > of judgment rather than simple diagnostic skill. Told to take as > > much time as I needed. I guess I passed - by coincidence, the entire > > group chanced to break up very quickly, and an entirely different team took over. > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > ************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From jmcgough <@t> clinlab.com Tue Jan 31 13:32:00 2012 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Tue Jan 31 13:32:09 2012 Subject: [Histonet] Factor XIIIa background staining problems In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3235D3D7C7@Mail2Node2.ad.uams.edu> Message-ID: We have been experiencing Factor XIIIa background staining problems and cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa clone EP3372 at a dilution of 1:250. We have tried many different protocols (i.e. enzyme pretreatment, high and low pH pretreatment, different incubation times, etc.)and nothing seems to fix this background staining. Our vendor told us that the dilution is good but we are questioning the actual dilution or the shelf life of the antibody. The antibody is a long ways from reaching the expiration date but we are still questioning it's stability. Has anybody else experienced this or know a solution? Thanks, Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From liz <@t> premierlab.com Tue Jan 31 13:35:54 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jan 31 13:36:03 2012 Subject: [Histonet] Factor XIIIa background staining problems In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5671@SBS2K8.premierlab.local> I would look at the antibody dilution and redo your titer study, your primary antibody is probably too concentrated. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough Sent: Tuesday, January 31, 2012 12:32 PM To: histonet@lists.utsouthwestern.edu Cc: Janna Hope Subject: [Histonet] Factor XIIIa background staining problems We have been experiencing Factor XIIIa background staining problems and cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa clone EP3372 at a dilution of 1:250. We have tried many different protocols (i.e. enzyme pretreatment, high and low pH pretreatment, different incubation times, etc.)and nothing seems to fix this background staining. Our vendor told us that the dilution is good but we are questioning the actual dilution or the shelf life of the antibody. The antibody is a long ways from reaching the expiration date but we are still questioning it's stability. Has anybody else experienced this or know a solution? Thanks, Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lapa <@t> cellmarque.com Tue Jan 31 13:41:03 2012 From: lapa <@t> cellmarque.com (Leslie Apa) Date: Tue Jan 31 13:41:13 2012 Subject: [Histonet] Factor XIIIa background staining problems In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA3235D3D7C7@Mail2Node2.ad.uams.edu>, Message-ID: <419DACA5-361B-483C-B3E6-F7879105C5EF@cellmarque.com> Hi Jason, I have been working with Janna on this issue and just got her email that The problem persists even after my suggestions. I can assure you the expiry is fine, many time the antibodies are still stable and usable even after the expiry date. Have you tried a variety of controls from different blocks? What is the lot number of this antibody? I will look further into this and see what else I can suggest. I may want to send you a couple stained and unstained slides to see if you can get staining on our tissue. We recommend a 1:250 dilution but that being said if you are still experiencing the background you have the flexibility of increasing the dilution to see if that helps. I will get back to you as soon as I can, but will need the lot number to check the device history. Thanks you, Leslie Apa Technical Consultant 916-746-8900 office 605-580-5333 mobile Sent from my iPhone On Jan 31, 2012, at 12:33 PM, "Jason McGough" wrote: > We have been experiencing Factor XIIIa background staining problems and > cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa > clone EP3372 at a dilution of 1:250. We have tried many different protocols > (i.e. enzyme pretreatment, high and low pH pretreatment, different > incubation times, etc.)and nothing seems to fix this background staining. > Our vendor told us that the dilution is good but we are questioning the > actual dilution or the shelf life of the antibody. The antibody is a long > ways from reaching the expiration date but we are still questioning it's > stability. Has anybody else experienced this or know a solution? > > Thanks, > > Jason McGough HT(ASCP) > Account Representative - Anatomic Pathology > Clinical Laboratory of the Black Hills > 2805 5th Street Suite 210 > Rapid City, SD 57701 > 605-343-2267 Ext 127 > 605-718-3779 (Fax) > jmcgough@clinlab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Jan 31 13:46:39 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 31 13:46:40 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3235D3D7C7@Mail2Node2.ad.uams.edu> References: , <41D3A1AF6FEF0643BDC89E0516A6EA3235D3D7C7@Mail2Node2.ad.uams.edu> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F80104@SMCMAIL01.somerset-healthcare.com> I too have had experience in both clinical and research settings. I have also interviewed applicants from both areas as well. The point made about new grads of on-line programs is something that I am now becoming more familiar with. We have our first student now, and she is doing very well. Her program started the beginning of the month, and I have given her more blocks to embed and cut than required by the program. We did have one person interview who graduated from one of these programs and had taken his HT exam. Unfortunately, his embedding and microtomy skills were not as good as that of even our current student. It is up to those of us who are fortunate enough to have students at our facilities, to give them plenty of opportunity to succeed not just on their written exams, but with actual hands-on training. Not just enough to submit a few slides at the end of the program, but to be able to keep a respectable pace while performing a variety of tasks. The applicant that we had who went through a program, obviously did not have much opportunity to get any extra bench time in. He explained to us some of the other tasks that he performed during his training, which is fine, and should be required, but it also took time away from essential skills that he needed to improve on. We debated quite a bit about whether we should hire this applicant. He seemed to be a good fit with staff, and was very eager to learn. The up side to this is that we could have trained him ourselves. The down side, was that we would have to take away tech time to spend with him, and not have him handle patient specimens until he was determined to be competent. At the time, we had a lot of vacation scheduled, and hiring him would have been more of an inconvenience than working short. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, January 31, 2012 2:06 PM To: 'Jerry Ricks'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Interviewing Histotechs... I have done both clinical and research and that comment happens in both areas about teaching me how to do that kind of work from doctors. Before you say monkeys maybe you should try it full time in clinical and find out what you are talking about. After 47 years I am still learning both areas as nothing is static and egos will get you in trouble. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Tuesday, January 31, 2012 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Interviewing Histotechs... Hi Toysha I think I'm just coming at it from "research mode" not clinical. Hands on Histotechnology is a core part of our work, but just part, and it is focused on animal models of cardiovascular disease. Depending on whether the researcher is a postdoc or an undergrad they will have more or fewer general lab skills including histo skills. I haven't met anyone yet who did not need some training for embedding of brachiocephalic arteries of mice. I doubt I would do well in a clinical lab. I've become accustomed to docs saying "wow that's beautiful can you teach me how to do that?" I gather in the clinical field it's more like "a monkey can learn how to section." Maybe a good monkey could but I doubt it could work up an IHC with a new antibody. Jerry > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 31 Jan 2012 10:38:58 -0600 > Subject: [Histonet] RE: Interviewing Histotechs... > > Jerry, > I agree with you somewhat. I have met techs that misrepresented themselves and said that they could cut or embed, and knew how to operate the instruments, but could not produce quality work. You are right when you said that it is different for clinical vs. research. I have almost always worked clinical, and noticed that when working with research techs, they had a difficult time adjusting to clinical with the time frames and quality. > When training new hires, depending on the position I am hiring for, I expect to train in the new workflow that they have learned, the new instrument they use, not the basic skills. I only expect to do that with a student. Fresh techs are expected to know how to get a section, not cut the plastic on the block, embed skin, and set up the h&e stainer. I should only have to go over and orient them on "our procedure" not teach the skill. > I have worked various part-time jobs over the years and the first thing I ask is 'how many microns do you cut at here'? While 3-4 is the standard, some labs want everything at 3, or some at 4. I know how to cut, but like you it takes about 2 weeks to get used to the new instrument. That's fine, but I don't expect to have to teach the tech how to embed a skin or cut a kidney biopsy. Not for an experienced tech, unless they have never encountered it. That has to be made known during the interview. > Yes, a cutting test is good, I have seen registered techs not make it past probation (90 days) because they could not cut. It would have saved the company time and MONEY if a test could have been given. Asking if they can cut a kidney biopsy, or embed a skin would be good as well. I can't go back and get more epithelia or ask for another pass through the kidney. > > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > > > Message: 5 > Date: Mon, 30 Jan 2012 11:13:11 -0800 > From: Jerry Ricks > Subject: [Histonet] Interviewing Histotechs... > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I gather it is different in clinical labs than in research labs. In clinical labs there is an emphasis on quantity and speed. In research the emphasis is on doing good experiments. Our "patients" are almost always deceased or shortly about to be so there is no urgency of diagnosis factor. For us, "diagnosis" means making precise measurements else some scientists looking at an image and asking each other "what the?" > > Anyway I always assume that the person I am hiring is incompetent at histology and that they will need to be personally trained by me. Doesn't matter how much experience they have. And over 23 years that has turned out to be true. I've met exactly two people who didn't need much training. One was a former senior clinical lab manager. The other was a kid straight out of high school who happened to have a histology experience from high school and a decent histo portfolio. Yes, Mercer Island High School had a Histology program. > > No such thing as a tech who doesn't need to be trained and any tech trained by me will be up and running in a week or two. Why bother making them cut or stain anything during a darn interview. If they are smart and cooperative they will work out. > > If I ever go to a new lab with a new microtome, new protocols, I am pretty sure that I will be sort of incompetent for a week or two as well. > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > > > histonet@lists.utsouthwestern.edu > > Date: Sun, 29 Jan 2012 13:12:09 -0500 > > From: rsrichmond@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re: interview.... > > > > Ray Koelling asked me: > > > > >>If the Samurai Pathologist is out there reading still; any idea > > >>over your career, about how many glass slides have you viewed > > >>under a microscope since the first? Your replies are always > > >>top-notch, entertaining and informative. And hope with each new > > >>job you don't have to show someone you can pass a test of which > > >>slide shows normal liver and which slide shows cirrhotic liver in > > >>your interview.<< > > > > I really have no idea how many slides. In a normal year I sign out > > about 3,000 histology cases (remember I don't work full time) > > averaging maybe 3 slides per case. > > > > Generally I've gotten jobs, both private clients and agency clients, > > by recommendation. A number of years ago I was interviewed by a > > four-pathologist hospital group who handed me a tray of 20 slides > > with the necessary historical information, and was told that this > > was a set the group had collected, including very straightforward > > cases, cases with serious diagnostic pitfalls, and some cases they'd > > never been able to make a diagnosis on. They tried to make it a test > > of judgment rather than simple diagnostic skill. Told to take as > > much time as I needed. I guess I passed - by coincidence, the entire > > group chanced to break up very quickly, and an entirely different team took over. > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > ************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Elizabeth.Cameron <@t> jax.org Tue Jan 31 13:57:19 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Tue Jan 31 13:57:28 2012 Subject: [Histonet] Processor Recomendations? Message-ID: Hi, I am looking for a reasonably-priced tissue processor for a research facility. We only process mouse tissue (100-200/day), and rapid processing is not something that is necessary. If possible, we would like the option of using isopropyl alcohol for processing. We currently have 2 Sakura VIPs that we are happy with, however, they are old and could go at any time. Any suggestions/recommendations would be appreciated. Thank you! Elizabeth M. Cameron, HT, QIHC (ASCP) From rjbuesa <@t> yahoo.com Tue Jan 31 14:30:43 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 31 14:30:51 2012 Subject: [Histonet] Processor Recomendations? In-Reply-To: Message-ID: <1328041843.51913.YahooMailClassic@web162102.mail.bf1.yahoo.com> I used to work with 3 VIPs, one of which was 15 years old. I really doubt that any of your VIPs "could go at any time". I suggest that the money you have invest in refurbishing your VIPs, a capital maintenance that the people from Sakura can do. I think you should go this way before investing in another instrument. Ren? J. --- On Tue, 1/31/12, Elizabeth Cameron wrote: From: Elizabeth Cameron Subject: [Histonet] Processor Recomendations? To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, January 31, 2012, 2:57 PM Hi, I am looking for a reasonably-priced tissue processor for a research facility.? We only process mouse tissue (100-200/day), and rapid processing is not something that is necessary.? If possible, we would like the option of using isopropyl alcohol for processing.? We currently have 2 Sakura VIPs that we are happy with, however, they are old and could go at any time.? Any suggestions/recommendations would be appreciated. Thank you! Elizabeth M. Cameron, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Tue Jan 31 14:40:52 2012 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Tue Jan 31 14:41:08 2012 Subject: [Histonet] Processor Recomendations? In-Reply-To: <1328041843.51913.YahooMailClassic@web162102.mail.bf1.yahoo.com> References: <1328041843.51913.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: Where is the LIKE button? Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 31, 2012 3:31 PM To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron Subject: Re: [Histonet] Processor Recomendations? I used to work with 3 VIPs, one of which was 15 years old. I really doubt that any of your VIPs "could go at any time". I suggest that the money you have invest in refurbishing your VIPs, a capital maintenance that the people from Sakura can do. I think you should go this way before investing in another instrument. Ren? J. --- On Tue, 1/31/12, Elizabeth Cameron wrote: From: Elizabeth Cameron Subject: [Histonet] Processor Recomendations? To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, January 31, 2012, 2:57 PM Hi, I am looking for a reasonably-priced tissue processor for a research facility.? We only process mouse tissue (100-200/day), and rapid processing is not something that is necessary.? If possible, we would like the option of using isopropyl alcohol for processing.? We currently have 2 Sakura VIPs that we are happy with, however, they are old and could go at any time.? Any suggestions/recommendations would be appreciated. Thank you! Elizabeth M. Cameron, HT, QIHC (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Jan 31 15:46:04 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Jan 31 15:46:13 2012 Subject: [Histonet] interview cutting-OT-disarmingly long fordeletiondisinterested References: <4F27A825.2B7F.00C9.1@geisinger.edu><1350046215.266731.1328019294499.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A7C6@UWHC-MAIL2.uwhis.hosp.wisc.edu> Perhaps an issue that hasn't been discussed yet as to why there are so may "posers" out there for histology jobs is that dispite the fact that in relation to other lab areas we get paid less, in these economic times a job in a hospital - anywhere is great pay and often good BENEFITS i.e. health insurance. Not to mention the great job security in Health Care as a whole. Anyone with half a degree in science can look up histology on the internet and think they can do the job (back to the "any monkey can do this argument" - I do NOT subscribe to this way of thinking). All they need to do is research a bit and think they can do the job. Desperate times you know. So for them it is worth a try. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Sours Sent: Tue 1/31/2012 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interview cutting-OT-disarmingly long fordeletiondisinterested For myself, I can say this: I can give you the best sections you've ever seen on my cryostat. But when I tried to use a cryostat that had disposable blades (in which you have to pick the section up from a completely different angle), I was terrible at first. I think you really need to get a technique down. It takes a few slides to get used to a microtome, at least a frozen one. Maybe with a paraffin microtome it's different because getting the section on the slide isn't about the knife angle and the slide. Either way, if I had to do sectioning on an interview, I would be so nervous, I'd probably do terribly. That said, thank god I have a job (for now!). Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that's beautiful. --Ron Swanson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Tue Jan 31 16:16:38 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jan 31 16:16:56 2012 Subject: [Histonet] RE: Processing adipose tissue In-Reply-To: <281CF76A690FA74783D6EF0EC4EF243C215F30@pbrcas30.pbrc.edu> References: <281CF76A690FA74783D6EF0EC4EF243C215F30@pbrcas30.pbrc.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A17AE6@xmdb02.nch.kids> Processing in IPA will give this picture. It is a lot gentler than ethanol/xylene. I would also increase the fixation time (remember fat does not fix well, being hydrophobic). We have had good success with 10% formalin in alcohol. I would not extend the processing steps at this time. I assume wax impregnation is at 60oC or more. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Tuesday, 31 January 2012 5:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing adipose tissue Esteemed experts, We have many clients who want to process mouse and human adipose tissue and are having some quality issues in the resultant slides. We have tried processing small chunks of tissue (<1 cm x 0.5 cm) on our automated processor (Excelsior) as follows: Tissue fixed for ~24 hrs in 10% NBF 70% Isopropyl alcohol (IPA) for 3 hrs 90% IPA, 3hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3 hrs 100% IPA, 3hrs Paraffin, 3 hrs Paraffin, 3 hrs Paraffin, 3 hrs Embed and section at 5 um prior to H&E. An example of what the sections look like can be found here http://imgur.com/7RTGR . We also ran a sample on a "traditional overnight" EtOH/Xylene processor (not at our facility) to compare results. That image is here: http://imgur.com/GjJPg . What is obvious is that the membranes in the IPA processed tissue seem to "flap over" and don't look as crisp as the Xylene processed tissue. We did notice structural defects in both samples (not shown) typically toward the middle of the specimens. Does anyone know what is causing our IPA processed fat to have these "wide membrane" artifacts? We are going to repeat the process with an additional 30 minutes per step and raise the temperature of the steps to ~ 35 C. We are also going to cut the blocks at 2-3 um to see if it can reduce the appearance of the membranes. Thanks very much for any advice you may have for us. We are pretty locked in to using xylene-free processing methodology if at all possible but will entertain any suggestions you may have. If I can provide any further details about what we are doing on our end, please let me know and I'll be happy to provide them. Best, David Burk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From algranth <@t> email.arizona.edu Tue Jan 31 17:06:57 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jan 31 17:07:07 2012 Subject: [Histonet] Processor Recomendations? In-Reply-To: References: <1328041843.51913.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: <97AA4AD3-2538-4D20-A7E5-E67B68EE67EB@email.arizona.edu> Stick with the VIP's - either your old ones or a refurbed newer model or even a NEW one if you have the money. I waited all my life for a VIP and I finally got one about 6 years ago and I just love it. I do animal tissue and have several different types of programs on the VIP (different embryo stages, different animal species brains, plants, insects, and routine too!) It has never failed and produces excellent processed tissues. Andi Grantham On Jan 31, 2012, at 1:40 PM, Helen Fedor wrote: > Where is the LIKE button? > > Helen > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, January 31, 2012 3:31 PM > To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron > Subject: Re: [Histonet] Processor Recomendations? > > I used to work with 3 VIPs, one of which was 15 years old. I really doubt that any of your VIPs "could go at any time". > I suggest that the money you have invest in refurbishing your VIPs, a capital maintenance that the people from Sakura can do. > I think you should go this way before investing in another instrument. > Ren? J. > > --- On Tue, 1/31/12, Elizabeth Cameron wrote: > > > From: Elizabeth Cameron > Subject: [Histonet] Processor Recomendations? > To: "histonet@lists.utsouthwestern.edu" > Date: Tuesday, January 31, 2012, 2:57 PM > > > Hi, > I am looking for a reasonably-priced tissue processor for a research facility. We only process mouse tissue (100-200/day), and rapid processing is not something that is necessary. If possible, we would like the option of using isopropyl alcohol for processing. We currently have 2 Sakura VIPs that we are happy with, however, they are old and could go at any time. Any suggestions/recommendations would be appreciated. > Thank you! > > Elizabeth M. Cameron, HT, QIHC (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Tue Jan 31 18:19:57 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 31 18:20:12 2012 Subject: [Histonet] RAC Medicare Audits - PAs Message-ID: <4F283EDD.7400.0077.1@harthosp.org> Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From michelecarr10 <@t> yahoo.com Tue Jan 31 20:09:09 2012 From: michelecarr10 <@t> yahoo.com (Michele Email) Date: Tue Jan 31 20:09:14 2012 Subject: [Histonet] Processor Recomendations? In-Reply-To: <97AA4AD3-2538-4D20-A7E5-E67B68EE67EB@email.arizona.edu> References: <1328041843.51913.YahooMailClassic@web162102.mail.bf1.yahoo.com> <97AA4AD3-2538-4D20-A7E5-E67B68EE67EB@email.arizona.edu> Message-ID: I agree VIP's are the way to go! Michele Carr Sent from my iPad On Jan 31, 2012, at 3:06 PM, "Grantham, Andrea L - (algranth)" wrote: > Stick with the VIP's - either your old ones or a refurbed newer model or even a NEW one if you have the money. I waited all my life for a VIP and I finally got one about 6 years ago and I just love it. I do animal tissue and have several different types of programs on the VIP (different embryo stages, different animal species brains, plants, insects, and routine too!) > It has never failed and produces excellent processed tissues. > > > Andi Grantham > > > > > On Jan 31, 2012, at 1:40 PM, Helen Fedor wrote: > >> Where is the LIKE button? >> >> Helen >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >> Sent: Tuesday, January 31, 2012 3:31 PM >> To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron >> Subject: Re: [Histonet] Processor Recomendations? >> >> I used to work with 3 VIPs, one of which was 15 years old. I really doubt that any of your VIPs "could go at any time". >> I suggest that the money you have invest in refurbishing your VIPs, a capital maintenance that the people from Sakura can do. >> I think you should go this way before investing in another instrument. >> Ren? J. >> >> --- On Tue, 1/31/12, Elizabeth Cameron wrote: >> >> >> From: Elizabeth Cameron >> Subject: [Histonet] Processor Recomendations? >> To: "histonet@lists.utsouthwestern.edu" >> Date: Tuesday, January 31, 2012, 2:57 PM >> >> >> Hi, >> I am looking for a reasonably-priced tissue processor for a research facility. We only process mouse tissue (100-200/day), and rapid processing is not something that is necessary. If possible, we would like the option of using isopropyl alcohol for processing. We currently have 2 Sakura VIPs that we are happy with, however, they are old and could go at any time. Any suggestions/recommendations would be appreciated. >> Thank you! >> >> Elizabeth M. Cameron, HT, QIHC (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet