[Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY

[Rene J Buesa]

To guarantee a "good section" two main issues have to be resolved: "good fixation" leading to "good infiltration".
"Good fixation" depends on the slices of tissue being as thin as possible and fixed during the adequate time to assure a complete fixation, especially if you use NBF.
If the "good fixation" is achieved, then your processing protocol has to assure a "good infiltration".
After that many "good" conditions I am sure you will end with a "good section".
Check all the steps in your work flow and check if all steps are as "good" as they should.
René J.

--- On Fri, 2/24/12, Wilson A <wilson6848 <@t> yahoo.com> wrote:


From: Wilson A <wilson6848 <@t> yahoo.com>
Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, February 24, 2012, 10:28 PM


 
    Hi,
       Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation.
   I will really, really appreciate if  you guys in histoland could suggest some solutions that could stop the problem.
   Hoping to read from you guys asap. You guys are the best.
 
Thanks,
 
Wilson
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