[Histonet] RE: Tissue adhesion

[McMahon, Loralee A]

What kind of slides are you using?   I have had bad lot numbers of slides.  New lot number cleared up the problem.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa [Melissa.Kuhnla <@t> chsli.org]
Sent: Friday, February 24, 2012 7:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue adhesion

Hello,
Happy Friday.
We are currently running IHC on Ventana XT and Ultra instrumentation.
We recently in the past couple of months have seen a rise in tissue
section loss.  Can anyone share with me their protocol for drying and
baking slides prior to IHC automated staining??

We had this problem in the past.  We found resolution by not
'over-processing' our small biopsies and treating our slides in certain
ways.  By that I mean we use adhesion slides, handle and cut all slides
with gloves worn, and dip control slides upside down to avoid altering
the change on the end of the slides that will hold the patient sample.
We currently dry slides for 15 minutes before baking in 60 degree oven
for 2 hours.

Thank you for any insight you may have.

Melissa Kuhnla
Lead Medical Technologist for IHC and FISH
Catholic Health Services of Long Island
Regional Laboratory Services



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