[Histonet] Re: Histonet Digest, Vol 99, Issue 28
Madeleine Huey
madeleinehuey <@t> gmail.com
Wed Feb 22 20:21:54 CST 2012
Message: 15
Date: Wed, 22 Feb 2012 15:26:43 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] Temp verifier slides - Ventana equipment
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="us-ascii"
Do y'all run the temp verifier slides for the quarterly maintenance
for the Ventana XT and Ultra? Those slides are mighty expensive to
buy every 3 months, esp if you have multiple pieces of equipment.
Amber,
We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work
just fine.
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Wed, Feb 22, 2012 at 10:01 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> Send Histonet mailing list submissions to
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>
> To subscribe or unsubscribe via the World Wide Web, visit
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> or, via email, send a message with subject or body 'help' to
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch)
> 2. RE: Processor Question (Davide Costanzo)
> 3. Job Opening in Orange county California (Paula Lucas)
> 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks)
> 5. Does xylene cause skin cancer? (Jenny Vega)
> 6. RE: Does xylene cause skin cancer? (Settembre, Dana)
> 7. Storage of Frozen Tissues (Dennis Hahn)
> 8. Re: Does xylene cause skin cancer? (Rene J Buesa)
> 9. RE: Does xylene cause skin cancer? (Debbie Faichney)
> 10. Re: Storage of Frozen Tissues (Rene J Buesa)
> 11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam)
> 12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam)
> 13. RE: Does xylene cause skin cancer? (Hilary Smith)
> 14. Histotech & Histology Supervisor Job in Naples, FL
> (Melissa Phelan)
> 15. Temp verifier slides - Ventana equipment (Amber McKenzie)
> 16. RE: Does xylene cause skin cancer? (Goins, Tresa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: 21 Feb 2012 13:08:28 -0500
> From: "Darcy Bloch" <darcyb <@t> slonepartners.com>
> Subject: [Histonet] Seeking a Histology Supervisor in Connecticut
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <mailman.0.1329933601.18415.histonet <@t> lists.utsouthwestern.edu>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Slone Partners seeks a Histology Supervisor for our hospital based
> laboratory
>
>
> The successful candidate redesign and be great at managing change. people in the department, including 2 supervisors. experience is a plus.
>
>
>
> Qualified certification, with high-volume laboratory.
>
>
>
> Special features of this position: have the opportunity to help redesign this
>
>
> If you meet these qualifications for this position, please submit your resume darcyb <@t> slonepartners.com.
>
>
>
> If you wish to be considered Tara Kochis at tara <@t> slonepartners.com.
>
>
>
> All inquiries are kept confidential.
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 21 Feb 2012 10:33:27 -0800
> From: Davide Costanzo <pathlocums <@t> gmail.com>
> Subject: RE: [Histonet] Processor Question
> To: "Gauch, Vicki" <GauchV <@t> mail.amc.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <-2296715788321707800 <@t> unknownmsgid>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Design flaw in the screen display. It is in the way of the chamber when
> opening chamber. If your not careful you will break the screen. Happens
> fairly often.
>
> Sent from my Windows Phone
> From: Gauch, Vicki
> Sent: 2/21/2012 9:18 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Processor Question
> Hi everyone,
> We are in the market for new processors...and I was wondering if
> anyone could give me some pros and cons for the Tissue Tek VIP 6
> tissue processor - how reliable are they? Ease of use ? Any known
> problems? Tissues process well? You know....all the usual questions
> we all ask for new equipment.....
>
> Thanks in advance for your help,
>
> Vicki Gauch
> AMCH
> Albany, NY
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 21 Feb 2012 12:23:07 -0800
> From: "Paula Lucas" <plucas <@t> biopath.org>
> Subject: [Histonet] Job Opening in Orange county California
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <B4B63414CF0349E7A008F0FD4B17E64C <@t> biopath.local>
> Content-Type: text/plain; charset="US-ASCII"
>
> A part-time histotech position is open in our histology laboratory.
>
> Tuesday through Saturday starting at 5 am.
>
> If anyone is interested, please either fax or email me your resume.
>
> Thank you,
>
> Paula Lucas
>
> Bio-Path Medical Group
>
> Fountain Valley, CA 92708
>
> Fax: 714-755-2984
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 21 Feb 2012 17:22:57 -0500
> From: Amos Brooks <amosbrooks <@t> gmail.com>
> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAC95ki_YVmP7TXrDFnRbsbtDh5-hfZ2Wc_m+LzimtFq_71NOcg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
> I haven't done a cellblock on CSF with Histogel, but I have has
> success with some fairly scanty cell culture specimens. A short processing
> cycle would be best. Try to make sure you have removed as much supernatant
> as possible to keep the gel from shriveling during processing. The IHC will
> be fine as long as the cells are suitably fixed ahead of time.
>
> Here is a link to the packaging insert for Histogel which describes
> preparation of cell blocks.
> https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf
>
> And here's a video of people using it for cytology specimens.
> http://tinyurl.com/7jb522e
>
> Amos
>
>
> On Tue, Feb 21, 2012 at 1:01 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
>> Message: 15
>> Date: Tue, 21 Feb 2012 11:26:10 -0600
>> From: "Turner, Leandra" <LTurner1 <@t> seton.org>
>> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <3D79F47DC92B204F9E5D35C885DFC5CB010AB869 <@t> AUSEX2VS1.seton.org>
>> Content-Type: text/plain; charset="us-ascii"
>>
>>
>>
>>
>>
>> Hello Everyone,
>>
>> I am trying to find out a few of things about making cell pellets on
>> cerebrospinal fluids. I would like to know:
>>
>>
>>
>> 1. If anyone has ever made cell pellets from CSF's using Histogel
>> and has any tips or procedures they could share?
>>
>>
>>
>> 2. How to process the CSF pellets made with Histogel, do we need a
>> routine or stat process? (we use a Sakura Tissue Tek)
>>
>>
>>
>> 3. Can you do IHC staining on the pellets?
>>
>>
>>
>>
>>
>> Thank you for any and all help in advance.
>>
>>
>>
>> Leandra Turner, HT (ASPC)CM
>>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 21 Feb 2012 23:17:58 -0400
> From: Jenny Vega <histotech411 <@t> gmail.com>
> Subject: [Histonet] Does xylene cause skin cancer?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CADyQr2Cv-VdN=mPfebQbQJGNZQ=XQeAbfa7tqUbtNhK45u2byA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> I am asking this because in my job we mount slides by hand, and my
> coworkers don't like to use gloves because it leaves a residue of latex in
> the back of the slides. I really don't feel comfortable mounting without
> gloves because I heard that xyelene can cause cancer. Some people I know
> personally has told me that this is not possible, but I read in some places
> that xylene could a possible carcinogen.
>
> I have already gotten contact with xylene in my hands a couple of times and
> I am worried.
>
>
>
> Thanks.
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 22 Feb 2012 06:47:20 -0500
> From: "Settembre, Dana" <settembr <@t> umdnj.edu>
> Subject: RE: [Histonet] Does xylene cause skin cancer?
> To: 'Jenny Vega' <histotech411 <@t> gmail.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B64B947688FB794A8C191D19F22927C80318E3F156 <@t> UMDEXMBX02.core.umdnj.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I would be worried too.
> Wearing gloves in the lab is always a good practice and
> I believe, a requirement in my lab whenever handling reagents or
> the possibility of coming into contact with reagents.
> You have the right to wear gloves if you want.
>
> Dana Settembre
> University Hospital - UMDNJ
> Newark, NJ
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jenny Vega
> Sent: Tuesday, February 21, 2012 10:18 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Does xylene cause skin cancer?
>
> I am asking this because in my job we mount slides by hand, and my
> coworkers don't like to use gloves because it leaves a residue of latex in
> the back of the slides. I really don't feel comfortable mounting without
> gloves because I heard that xyelene can cause cancer. Some people I know
> personally has told me that this is not possible, but I read in some places
> that xylene could a possible carcinogen.
>
> I have already gotten contact with xylene in my hands a couple of times and
> I am worried.
>
>
>
> Thanks.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 22 Feb 2012 12:20:02 +0000
> From: Dennis Hahn <Dennis.Hahn <@t> cookchildrens.org>
> Subject: [Histonet] Storage of Frozen Tissues
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DB0B6557A0E64E448202D8C0F86538B315ED08D6 <@t> CCHCSMBX01.CCHCS.LDAP>
> Content-Type: text/plain; charset="us-ascii"
>
> How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas?
>
> Thanks,
> Dennis
>
> Dennis Hahn, HT (ASCP)
> Histology Lab Supervisor
> Cook Children's Medical Center
> 801 7th Avenue
> Ft. Worth, TX 76104
> (682) 885-6168
>
>
>
> ________________________________
>
> ----------------------------------------------------------------------------------------
> Cook Children's Health Care System
>
> This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations.
>
> If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance <@t> cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.
> ---------------------------------------------------------------------------------------
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 22 Feb 2012 05:08:51 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Does xylene cause skin cancer?
> To: histonet <@t> lists.utsouthwestern.edu, Jenny Vega
> <histotech411 <@t> gmail.com>
> Message-ID:
> <1329916131.63705.YahooMailClassic <@t> web162101.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented.
> Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them.
> René J.
>
> --- On Tue, 2/21/12, Jenny Vega <histotech411 <@t> gmail.com> wrote:
>
>
> From: Jenny Vega <histotech411 <@t> gmail.com>
> Subject: [Histonet] Does xylene cause skin cancer?
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, February 21, 2012, 10:17 PM
>
>
> I am asking this because in my job we mount slides by hand, and my
> coworkers don't like to use gloves because it leaves a residue of latex in
> the back of the slides. I really don't feel comfortable mounting without
> gloves because I heard that xyelene can cause cancer. Some people I know
> personally has told me that this is not possible, but I read in some places
> that xylene could a possible carcinogen.
>
> I have already gotten contact with xylene in my hands a couple of times and
> I am worried.
>
>
>
> Thanks.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 22 Feb 2012 13:15:28 +0000
> From: Debbie Faichney <d.a.faichney <@t> stir.ac.uk>
> Subject: RE: [Histonet] Does xylene cause skin cancer?
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Jenny Vega
> <histotech411 <@t> gmail.com>
> Message-ID:
> <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A <@t> EXCH2007.ad.stir.ac.uk>
> Content-Type: text/plain; charset="iso-8859-1"
>
> If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough.
>
> Debbie Faichney
> Institute of Aquaculture
> University of Stirling
> UK
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: 22 February 2012 13:09
> To: histonet <@t> lists.utsouthwestern.edu; Jenny Vega
> Subject: Re: [Histonet] Does xylene cause skin cancer?
>
> There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented.
> Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them.
> René J.
>
> --- On Tue, 2/21/12, Jenny Vega <histotech411 <@t> gmail.com> wrote:
>
>
> From: Jenny Vega <histotech411 <@t> gmail.com>
> Subject: [Histonet] Does xylene cause skin cancer?
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, February 21, 2012, 10:17 PM
>
>
> I am asking this because in my job we mount slides by hand, and my
> coworkers don't like to use gloves because it leaves a residue of latex in
> the back of the slides. I really don't feel comfortable mounting without
> gloves because I heard that xyelene can cause cancer. Some people I know
> personally has told me that this is not possible, but I read in some places
> that xylene could a possible carcinogen.
>
> I have already gotten contact with xylene in my hands a couple of times and
> I am worried.
>
>
>
> Thanks.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> --
> The Sunday Times Scottish University of the Year 2009/2010
> The University of Stirling is a charity registered in Scotland,
> number SC 011159.
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 22 Feb 2012 05:16:27 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Storage of Frozen Tissues
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>, Dennis Hahn
> <Dennis.Hahn <@t> cookchildrens.org>
> Message-ID:
> <1329916587.26606.YahooMailClassic <@t> web162104.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Tissues in my tumor bank (-80ºC) were kept in small (1inchx2inches) labeled zip-lock bags. I do not understand about your concern regarding "tissues being exposed to staff".
> Inside the bags I used, the tissues were not exposed. If they were taken out for any study or procedures, universal precautions were taken.
> René J.
>
> --- On Wed, 2/22/12, Dennis Hahn <Dennis.Hahn <@t> cookchildrens.org> wrote:
>
>
> From: Dennis Hahn <Dennis.Hahn <@t> cookchildrens.org>
> Subject: [Histonet] Storage of Frozen Tissues
> To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, February 22, 2012, 7:20 AM
>
>
> How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas?
>
> Thanks,
> Dennis
>
> Dennis Hahn, HT (ASCP)
> Histology Lab Supervisor
> Cook Children's Medical Center
> 801 7th Avenue
> Ft. Worth, TX 76104
> (682) 885-6168
>
>
>
> ________________________________
>
> ----------------------------------------------------------------------------------------
> Cook Children's Health Care System
>
> This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations.
>
> If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance <@t> cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.
> ---------------------------------------------------------------------------------------
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 22 Feb 2012 05:21:47 -0800 (PST)
> From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
> Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel
> To: "Turner, Leandra" <LTurner1 <@t> seton.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1329916907.78300.YahooMailNeo <@t> web130104.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material. Here is my procedure:
>
>
> 1. you need about 5X10^ cells per pellet
> 2. spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube
> 3. aspirate and resuspend in 15ml NBF and fix overnight @ RT
> 4. spin @ 2000 rpm for 5 minutes and aspirate the NBF
> 5. microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving)
> 6. histogel should now be at liquid for and about 60C
> 7. resuspend cell pellet in about 350ul of histogel
> 8. place cell pellet in freezer or on ice for about 20 minutes to solidify
> 9. using spatula, remove histogel pellet and place into cassette
> 10. post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed
> 11. process as usual
>
>
>
> Good luck!
>
> Kim
> Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA
>
>
> ________________________________
> From: "Turner, Leandra" <LTurner1 <@t> seton.org>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Tuesday, February 21, 2012 12:26 PM
> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
>
>
>
>
>
> Hello Everyone,
>
> I am trying to find out a few of things about making cell pellets on
> cerebrospinal fluids. I would like to know:
>
>
>
> 1. If anyone has ever made cell pellets from CSF's using Histogel
> and has any tips or procedures they could share?
>
>
>
> 2. How to process the CSF pellets made with Histogel, do we need a
> routine or stat process? (we use a Sakura Tissue Tek)
>
>
>
> 3. Can you do IHC staining on the pellets?
>
>
>
>
>
> Thank you for any and all help in advance.
>
>
>
> Leandra Turner, HT (ASPC)CM
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 12
> Date: Wed, 22 Feb 2012 05:23:15 -0800 (PST)
> From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
> Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel
> To: "Turner, Leandra" <LTurner1 <@t> seton.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1329916995.72580.YahooMailNeo <@t> web130102.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I forgot to mention, we prepare these specifically for IHC; so yes, you can do IHC on them.
> Kim
>
> Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA
>
>
> ________________________________
> From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
> To: "Turner, Leandra" <LTurner1 <@t> seton.org>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, February 22, 2012 8:21 AM
> Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel
>
>
> I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material. Here is my procedure:
>
>
> 1. you need about 5X10^ cells per pellet
> 2. spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube
> 3. aspirate and resuspend in 15ml NBF and fix overnight @ RT
> 4. spin @ 2000 rpm for 5 minutes and aspirate the NBF
> 5. microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving)
> 6. histogel should now be at liquid for and about 60C
> 7. resuspend cell pellet in about 350ul of histogel
> 8. place cell pellet in freezer or on ice for about 20 minutes to solidify
> 9. using spatula, remove histogel pellet and place into cassette
> 10. post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed
> 11. process as usual
>
>
>
> Good luck!
>
> Kim
> Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA
>
>
> ________________________________
> From: "Turner, Leandra" <LTurner1 <@t> seton.org>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Tuesday, February 21, 2012 12:26 PM
> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
>
>
>
>
>
> Hello Everyone,
>
> I am trying to find out a few of things about making cell pellets on
> cerebrospinal fluids. I would like to know:
>
>
>
> 1. If anyone has ever made cell pellets from CSF's using Histogel
> and has any tips or procedures they could share?
>
>
>
> 2. How to process the CSF pellets made with Histogel, do we need a
> routine or stat process? (we use a Sakura Tissue Tek)
>
>
>
> 3. Can you do IHC staining on the pellets?
>
>
>
>
>
> Thank you for any and all help in advance.
>
>
>
> Leandra Turner, HT (ASPC)CM
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 13
> Date: Wed, 22 Feb 2012 14:51:31 +0000
> From: Hilary Smith <hsmith <@t> wakehealth.edu>
> Subject: RE: [Histonet] Does xylene cause skin cancer?
> To: Debbie Faichney <d.a.faichney <@t> stir.ac.uk>, Rene J Buesa
> <rjbuesa <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Jenny Vega
> <histotech411 <@t> gmail.com>
> Message-ID:
> <C45CEA76F49F3145ABAA49459E6DE22B030BC460 <@t> exchdb5.medctr.ad.wfubmc.edu>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene:
>
> http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx
>
> According to our xylene MSDS: "The substance may be toxic to blood, kidneys, liver,
> mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can
> produce target organs damage."
>
> I would definitely use gloves if I were you.
>
> Hilary
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debbie Faichney
> Sent: Wednesday, February 22, 2012 8:15 AM
> To: Rene J Buesa; histonet <@t> lists.utsouthwestern.edu; Jenny Vega
> Subject: RE: [Histonet] Does xylene cause skin cancer?
>
> If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough.
>
> Debbie Faichney
> Institute of Aquaculture
> University of Stirling
> UK
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: 22 February 2012 13:09
> To: histonet <@t> lists.utsouthwestern.edu; Jenny Vega
> Subject: Re: [Histonet] Does xylene cause skin cancer?
>
> There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented.
> Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them.
> René J.
>
> --- On Tue, 2/21/12, Jenny Vega <histotech411 <@t> gmail.com> wrote:
>
>
> From: Jenny Vega <histotech411 <@t> gmail.com>
> Subject: [Histonet] Does xylene cause skin cancer?
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, February 21, 2012, 10:17 PM
>
>
> I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen.
>
> I have already gotten contact with xylene in my hands a couple of times and I am worried.
>
>
>
> Thanks.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> --
> The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 22 Feb 2012 10:00:59 -0500
> From: Melissa Phelan <melissa <@t> alliedsearchpartners.com>
> Subject: [Histonet] Histotech & Histology Supervisor Job in Naples, FL
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <CB6A6D5B.36A8F%melissa <@t> alliedsearchpartners.com>
> Content-Type: text/plain; charset="ISO-8859-1"
>
> Allied Search Partners has been retained for the following searches in
> Florida. Please forward this along to anyone who you know that would be
> interested in any of the following positions. We do offer a referral bonus.
>
> Please email a copy of updated resume to melissa <@t> alliedsearchpartners.com
> <mailto:brannon <@t> alliedsearchpartners.com> for a full job description.
>
> We have the following positions available:
>
>
> Histotech
> LOCATION: Naples, FL
> DEPARTMENT & SCHEDULE:
> Monday-Friday Day Shift/Full Time (6:30am-2:30pm)
>
> Histology Supervisor
> LOCATION: Naples, FL
> DEPARTMENT & SCHEDULE:
> *Bachelor¹s Degree Required, FL Histology Supervisor License Eligible
> Monday-Friday Day Shift/Full Time
>
> --
> Melissa Phelan, President Laboratory Staffing
> Allied Search Partners
> http://www.linkedin.com/in/melissaphelan
> <http://www.linkedin.com/in/melissaphelan> P: 888-388-7571
> F: 888-388-7572
> C: 407-697-1175
> www.alliedsearchpartners.com
>
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 22 Feb 2012 15:26:43 +0000
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] Temp verifier slides - Ventana equipment
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E <@t> JERRY.Gia.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment.
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 22 Feb 2012 17:14:40 +0000
> From: "Goins, Tresa" <TGoins <@t> mt.gov>
> Subject: RE: [Histonet] Does xylene cause skin cancer?
> To: Jenny Vega <histotech411 <@t> gmail.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CA4DF32ED505D94BB55E95487D8E98411F1D7C68 <@t> DOAISD5205.state.mt.ads>
> Content-Type: text/plain; charset="us-ascii"
>
> Nitrile gloves are recommended for all the chemicals we use in our lab except acetone - for acetone latex is recommended.
> We also coverslip by hand but we wear nitrile gloves without exception.
>
> Tresa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jenny Vega
> Sent: Tuesday, February 21, 2012 8:18 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Does xylene cause skin cancer?
>
> I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen.
>
> I have already gotten contact with xylene in my hands a couple of times and I am worried.
>
>
>
> Thanks.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 99, Issue 28
> ****************************************
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