[Histonet] questions

[Webb, Dorothy L]

For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc??

How long are unstained slides usable?  Do any of you pick up extra sections from a ribbon if the tissue is minimal ?  We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block.

Appreciate ahead of time your responses!

Dorothy Webb, HT (ASCP)
Regions Hospital



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