[Histonet] Cytology CSF Cell Pellets made from Histogel

[Amos Brooks]

Hi,
     I haven't done a cellblock on CSF with Histogel, but I have has
success with some fairly scanty cell culture specimens. A short processing
cycle would be best. Try to make sure you have removed as much supernatant
as possible to keep the gel from shriveling during processing. The IHC will
be fine as long as the cells are suitably fixed ahead of time.

Here is a link to the packaging insert for Histogel which describes
preparation of cell blocks.
https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf

And here's a video of people using it for cytology specimens.
http://tinyurl.com/7jb522e

Amos


On Tue, Feb 21, 2012 at 1:01 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 15
> Date: Tue, 21 Feb 2012 11:26:10 -0600
> From: "Turner, Leandra" <LTurner1 <@t> seton.org>
> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
> To: <histonet <@t> lists.utsouthwestern.edu>
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>        <3D79F47DC92B204F9E5D35C885DFC5CB010AB869 <@t> AUSEX2VS1.seton.org>
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>
> Hello Everyone,
>
>    I am trying to find out a few of things about making cell pellets on
> cerebrospinal fluids.  I would like to know:
>
>
>
>     1. If anyone has ever made cell pellets from CSF's using Histogel
> and has any tips or procedures they could share?
>
>
>
>     2. How to process the CSF pellets made with Histogel, do we need a
> routine or stat process?  (we use a Sakura Tissue Tek)
>
>
>
>     3. Can you do IHC staining on the pellets?
>
>
>
>
>
> Thank you for any and all help in advance.
>
>
>
> Leandra Turner, HT (ASPC)CM
>