[Histonet] Humidity levels and IHC staining

[Britton, Josette C]

The Bond Max from Leica never has this problem!

 

Josie Britton HT

Cheshire Medical Center

Keene, NH 03431

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, February 17, 2012 2:27 PM
To: 'Rene J Buesa'; histonet <@t> lists.utsouthwestern.edu; Jason McGough
Subject: RE: [Histonet] Humidity levels and IHC staining

 

Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems.

 

Tim Morken

Supervisor, Histology, IPOX

UCSF Medical Center

San Francisco, CA, USA

 

-----Original Message-----

From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa

Sent: Friday, February 17, 2012 11:20 AM

To: histonet <@t> lists.utsouthwestern.edu; Jason McGough

Subject: Re: [Histonet] Humidity levels and IHC staining

 

A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer.

Which auto stainer are you using?

René J. 

 

--- On Fri, 2/17/12, Jason McGough <jmcgough <@t> clinlab.com> wrote:

 

 

From: Jason McGough <jmcgough <@t> clinlab.com>

Subject: [Histonet] Humidity levels and IHC staining

To: histonet <@t> lists.utsouthwestern.edu

Date: Friday, February 17, 2012, 2:09 PM

 

 

We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated.

 

Jason McGough HT(ASCP)

Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills

2805 5th Street Suite 210

Rapid City, SD 57701

605-343-2267 Ext 127

605-718-3779 (Fax)

jmcgough <@t> clinlab.com

 

 

 

 

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