[Histonet] Humidity levels and IHC staining

[Jason McGough]

We use the Dako Autostainer.

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough <@t> clinlab.com

  -----Original Message-----
  From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
  Sent: Friday, February 17, 2012 12:20 PM
  To: histonet <@t> lists.utsouthwestern.edu; Jason McGough
  Subject: Re: [Histonet] Humidity levels and IHC staining


        A good auto stainer (like DAKO) with adequate amounts of dispensed
reagents during the correct periods of time should not experiment any drying
out on the slides. Adequate humidity is required to be controlled during
manual IHC, especially if done over a heated support. If because of any
reason (including not leveled slides) you experiment drying out, the best
way would be to have an open flat dish containing water but, again, that was
never a problem for me using the DAKO auto stainer.
        Which auto stainer are you using?
        René J.

        --- On Fri, 2/17/12, Jason McGough <jmcgough <@t> clinlab.com> wrote:


          From: Jason McGough <jmcgough <@t> clinlab.com>
          Subject: [Histonet] Humidity levels and IHC staining
          To: histonet <@t> lists.utsouthwestern.edu
          Date: Friday, February 17, 2012, 2:09 PM


          We are wondering what other labs are doing to control the humidity
while IHC
          stains are being performed. We currently place wet towels and a
small weigh
          boat with water in our Autostainer to help prevent our slides from
drying
          out but that seems to not be enough, they still tend to dry out
and produce
          background staining. What should the humidity level be at? Any
help would be
          appreciated.

          Jason McGough HT(ASCP)
          Account Representative - Anatomic Pathology
          Clinical Laboratory of the Black Hills
          2805 5th Street Suite 210
          Rapid City, SD 57701
          605-343-2267 Ext 127
          605-718-3779 (Fax)
          jmcgough <@t> clinlab.com




          _______________________________________________
          Histonet mailing list
          Histonet <@t> lists.utsouthwestern.edu
          http://lists.utsouthwestern.edu/mailman/listinfo/histonet