[Histonet] Ribbon won't stay on knife

[Rathborne, Toni]

I also find it helpful to blunt the bottom edge of the block by scraping to create two corners/angles on it instead of one 90 degree angle.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 16, 2012 6:16 AM
To: 'Jerry Ricks'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Ribbon won't stay on knife

I use my forceps to round over the top edge of the block when this happens.  If you ever have trouble getting a ribbon to form because the sections do not stick together, you can also use the forceps to straighten the bottom edge of the block.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jerry Ricks
Sent: Wednesday, February 15, 2012 6:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ribbon won't stay on knife


Sometimes while cutting 5 micron paraffin sections...

I turn the wheel once and a nice section gets cut and lays down on my blade.  When I turn the wheel again, as the knife holder is traveling Upwards, the top of my paraffin block will grab the ribbon and lift it off of the microtome blade, ruining my ribbon. Any ideas what is going on, how to fix it.

Today I'm using my old trust Spencer 820 microtome, but it also sometimes happens on my Leica RM2125.

Thanks,

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology


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