[Histonet] Re: bone implants

[Reynolds,Donna M]

I don't know about implants but we were having a terrible time with bone marrows coming off. Usually they came off during the HEIR using the steamer.
At that point we were placing our slides lying flat in a 55-60º oven overnight which usually helps tremendously with difficult tissue. This was followed with routine deparaffinization and hydration. It was suggested to us to try a 70º water bath for 2-4 hours for HEIR. I was doubtful that my antibodies would work but they did. In fact the staining was better with 2 hr HEIR than with 4. 

 Donna Reynolds Chief Histology laboratory, Research
Department Cancer Biology
U.T. M.D. Anderson Cancer Center, Houston, TX.
713-792-8106

Message: 4
Date: Mon, 13 Feb 2012 09:53:15 -0600
From: "del phillips" <dphillips <@t> vetmed.lsu.edu>
Subject: [Histonet] IHC and bone implants
To: <Histonet <@t> lists.utsouthwestern.edu>
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Does anyone have any suggestions for staining bone implants? I am having
trouble with wash offs. I use charged slides and they are still washing off.

 

Thanks

Del