[Histonet] AFB control

[Jennifer MacDonald]

Carson




"Mike Pence" <mpence <@t> grhs.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/10/2012 12:49 PM

To
"Jennifer MacDonald" <JMacDonald <@t> mtsac.edu>, "Rene J Buesa" 
<rjbuesa <@t> yahoo.com>
cc
histonet <@t> lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 
<srhthacker <@t> hotmail.com>, histonet-bounces <@t> lists.utsouthwestern.edu
Subject
RE: [Histonet] AFB control






Recommended by who?
Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Friday, February 10, 2012 2:43 PM
To: Rene J Buesa
Cc: histonet <@t> lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB control


It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 

the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 

and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa <rjbuesa <@t> yahoo.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/10/2012 11:55 AM

To
histonet <@t> lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 
<srhthacker <@t> hotmail.com>
cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker <srhthacker <@t> hotmail.com> 
wrote:


From: Stephanie Hoyle-Thacker <srhthacker <@t> hotmail.com>
Subject: [Histonet] AFB control
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker 
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