[Histonet] reply to message 6
M. Kap
m.kap.1 <@t> erasmusmc.nl
Thu Feb 9 15:27:34 CST 2012
check wwww.cqpath.com : all you wanted to know about IHC but were afraid
to ask ;-)
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> Today's Topics:
>
> 1. Temporary Tech Agencies (Jennifer MacDonald)
> 2. cryostats decontamination (anita dudley)
> 3. Revco -80 Freezer Sliding Drawer Rack Reviews (Lucie Guernsey)
> 4. EGFR ISH protocol for Ventana (Lanigan, Christopher)
> 5. New Histology Positions - Northern IL - Retained Search
> (Matt Ward)
> 6. QIHC (Sarah Dysart)
> 7. RE: QIHC (Walter Benton)
> 8. exit strategy for staff (Edwards, Richard E.)
> 9. (no subject) (Freeman, Carol)
> 10. Flammable / explosion proof refrigerator (angela smith)
> 11. RE: Flammable / explosion proof refrigerator (Morken, Timothy)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 8 Feb 2012 09:40:10 -0800
> From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Subject: [Histonet] Temporary Tech Agencies
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF17BAC925.14142466-ON8825799E.0060F27D-8825799E.00611E87 <@t> mtsac.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hi All,
> I have a lab that is in need of temporary help for a maternity leave. Does
> anyone know of a good temporary agency?
> Thanks,
> Jennifer MacDonald
>
> ------------------------------
>
> Message: 2
> Date: Wed, 8 Feb 2012 16:49:29 -0600
> From: anita dudley <azdudley <@t> hotmail.com>
> Subject: [Histonet] cryostats decontamination
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU163-W55B7DEA6E8729D2249FC95D17A0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Is everyone taking their crostats down weekly?? just seems overkill to me
>
> thanks,
> anita dudley
>
> ------------------------------
>
> Message: 3
> Date: Wed, 8 Feb 2012 15:07:09 -0800
> From: Lucie Guernsey <lguernsey <@t> ucsd.edu>
> Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CANQOiZ3D1wVb-aV5orfCJ2kE8MRAABdtNUZUaXgi8L5_1dmpKg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi all,
>
> We're going to be purchasing a Revco UxF -86 C upright freezer soon and
> we'd like to fill it with 2" box racks. Revco offers 2 options: the
> standard side-access racks and sliding drawer racks. The idea of the
> sliding drawer racks sounds great (you don't have to pull the entire rack
> out of the freezer, just a drawer), but I'm wondering if, in practice,
> they're not as great as they sound. I imagine they could freeze up, be
> hard
> to slide, etc. I have yet to find any reviews regarding the racks online,
> so I thought I'd come to you guys. Does anyone use the sliding drawer
> racks
> and have an opinion about them? Should we just stick with the tried and
> true side-access racks?
>
> Thanks for your input!
> Lucie
>
> Lucie Guernsey
> UC San Diego
> lguernsey <@t> ucsd.edu
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 9 Feb 2012 07:02:05 -0500
> From: "Lanigan, Christopher" <lanigac <@t> ccf.org>
> Subject: [Histonet] EGFR ISH protocol for Ventana
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <9DDD0F026DC71B41B79D8E439D7951A30BAB4791 <@t> cchsclexmb69.cc.ad.cchs.net>
> Content-Type: text/plain; charset=us-ascii
>
> ------Original message ----------------------------------------
>
>
>
> Hi
>
>
>
> Can someone help me with the EGFR ISH protocol for Ventana?
>
>
>
> Thanks!
>
>
>
> Mei Zheng, HTL(ASCP) QIHC
>
> Clinical Supervisor
>
> Immunohistochemistry Lab.
>
> Department of Pathology
>
> Brigham and Women's Hospital
>
> Boston, MA 02115
>
> Lab Tel: 617 732-7790
>
>
>
> ------Reply ----------------------------------------
>
>
>
> Hi Mei,
>
>
>
> Sorry for the delay.
>
>
>
> Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the
> Ventana Benchmark XT.
>
>
>
> First, the software that you will need to have installed is called "XT
> Dual Color Open Probe". This protocol will use the UltraView Silver ISH
> DNP Detection for EGFR and UltraView Red ISH DIG Detection for
> Chromosome 7. The counterstain is Hematoxylin II with Bluing Reagent.
>
>
>
> Before I get to the protocol, I'll fill you in on the EGFR and
> Chromosome 7 probes. They do NOT arrive in a dispenser. They each
> arrive in pre-diluted vials, so you will need to fill a "user-fillable
> dispenser". The pre-diluted vials are mixed together into one
> user-fillable dispenser to create a "cocktail".
>
>
>
> Don't forget you will also need a "button kit" for "ISH PROBE" because
> the user-fillable dispenser will not have the proper commercial bar
> code.
>
>
>
> One more thing, UltraView Red ISH DIG Detection cannot be dehydrated in
> alcohol. I usually dry the slides at 65C for an hour before I
> coverslip.
>
>
>
> Finally, the following is a successful protocol summary.
>
>
>
> 1 Deparaffinization [Selected]
>
>
>
> 2 Extended [Selected]
>
>
>
> 3 Warmup Slide to [72 Deg C], and Incubate for 4 Minutes (
> Deparaffinization )
>
>
>
> 4 Cell Conditioning [Selected]
>
>
>
> 5 CC2 [Selected]
>
>
>
> 6 CC2 Cycle 1 [Selected]
>
>
>
> 7 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Cycle 1 )
>
>
>
> 8 CC2 Cycle 2 [Selected]
>
>
>
> 9 Incubate for [12 Minutes] ( Cycle 2 )
>
>
>
> 10 CC2 Cycle 3 [Selected]
>
>
>
> 11 Incubate for [12 Minutes] ( Cycle 3 )
>
>
>
> 12 Enzyme [Selected]
>
>
>
> 13 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), Apply Coverslip, and
> Incubate for [32 Minutes]
>
>
>
> 14 Probe Auto Dispense [Selected]
>
>
>
> 15 Apply One Drop of [ISH Probe 3] ( ISH Probe ), No Coverslip and
> Incubate for 8 Minutes
>
>
>
> 16 Denature [Selected]
>
>
>
> 17 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] (
> Denaturation )
>
>
>
> 18 Hybridization [Selected]
>
>
>
> 19 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes (
> Hybridization )
>
>
>
> 20 Incubate for [6 Hours] ( Hybridization )
>
>
>
> 21 Stringency Washes [Selected]
>
>
>
> 22 Stringency Wash #1 [Selected]
>
>
>
> 23 Warmup Slide to [72 Deg C], and Incubate for [8 Minutes] ( Stringency
> Wash #1 )
>
>
>
> 24 Stringency Wash #2 [Selected]
>
>
>
> 25 Incubate for [8 Minutes] ( Stringency Wash #2 )
>
>
>
> 26 Stringency Wash #3 [Selected]
>
>
>
> 27 Incubate for [8 Minutes] ( Stringency Wash #3 )
>
>
>
> 28 Silver Detection [Selected]
>
>
>
> 29 Apply One Drop of SIL ISH DNP RAB, Apply Coverslip, and Incubate for
> [20 Minutes]
>
>
>
> 30 Apply One Drop of SIL ISH DNP CHRC, and Incubate for [4 Minutes]
>
>
>
> 31 Red Detection [Selected]
>
>
>
> 32 Apply One Drop of RED ISH DIG MAB, Apply Coverslip, and Incubate for
> [20 Minutes]
>
>
>
> 33 Apply One Drop of RED ISH DIG FR, and Incubate for [4 Minutes]
>
>
>
> 34 Counterstain [Selected]
>
>
>
> 35 Apply One Drop of [HEMATOXYLIN II] ( Counterstain ), Apply Coverslip,
> and Incubate for [8 Minutes]
>
>
>
> 36 Post Counterstain [Selected]
>
>
>
> 37 Apply One Drop of [BLUING REAGENT] ( Post Counterstain ), Apply
> Coverslip, and Incubate for [4 Minutes]
>
>
>
> 38 Post Run LCS Application [Selected]
>
>
>
>
>
> Christopher Lanigan
>
> Research Technologist
>
> Molecular Pathology
>
> Cleveland Clinic Foundation
>
> 9500 Euclid Avenue L3-127
>
> Cleveland, OH 44195
>
>
>
>
> ===================================
>
> Please consider the environment before printing this e-mail
>
> Cleveland Clinic is ranked one of the top hospitals
> in America by U.S.News & World Report (2010).
> Visit us online at http://www.clevelandclinic.org for
> a complete listing of our services, staff and
> locations.
>
>
> Confidentiality Note: This message is intended for use
> only by the individual or entity to which it is addressed
> and may contain information that is privileged,
> confidential, and exempt from disclosure under applicable
> law. If the reader of this message is not the intended
> recipient or the employee or agent responsible for
> delivering the message to the intended recipient, you are
> hereby notified that any dissemination, distribution or
> copying of this communication is strictly prohibited. If
> you have received this communication in error, please
> contact the sender immediately and destroy the material in
> its entirety, whether electronic or hard copy. Thank you.
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 9 Feb 2012 10:00:59 -0500
> From: Matt Ward <mw <@t> personifysearch.com>
> Subject: [Histonet] New Histology Positions - Northern IL - Retained
> Search
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8b2c63aac6f85a2c063ac069a23a1e27 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Good Morning,
>
>
>
> Personify has had 2 new permanent Histology opportunities become available
> in the Northern IL area. The positions offer competitive packages and the
> opportunity for career growth.
>
>
>
> Please contact me directly at mw <@t> personifysearch to learn more.
>
>
>
> *The Company:*
>
> Well-established provider of consumables and medical device accessories
> for
> clinical histology and research laboratories. The facility works closely
> with our UK, German and Australian facilities in the development,
> manufacture and marketing of products including processing reagents,
> storage and specimen transport devices, cytology accessories and safety
> products.
>
> This is a globally focused business with significant sales and operations
> in the US, Europe and Asia Pacific as well as a direct presence in over
> 100
> countries.
>
> *The Opportunities:*
>
> (1) QA Histologist: The company currently has an opening for a Quality
> Assurance Histologist to be based in Richmond IL.
>
> (2) Histologist: The company currently has an opening for a Histologist
> working on the R&D team to be based in Richmond IL.
>
> Salary: Based on Experience
>
> Other: Full benefits - 401k program/matching
>
>
> *Education and Experience Required:*
>
> - Experience with tissue grossing, tissue processing and embedding
> - Experience with sectioning paraffin embedded tissue as well as frozen
> tissue
> - Experience performing routine stains (H and E) as well as special stains
> - Experience with formulation and production of routine laboratory
> reagents
> and solutions
> - Experience performing and documenting routine laboratory procedures
> - Familiarity with compliance requirements in the medical device industry
> - Proficiency in basic computer skills and with software applications such
> as Microsoft Office
>
> Have a great day!
>
>
>
>
>
> Matt Ward
>
> *Account Executive*
>
> *Personify*
>
> 5020 Weston Parkway Suite 315
>
> Cary NC 27513
>
> (Tel) 800.875.6188 direct ext 103
>
> (Fax) 919.460.0642
>
> www.personifysearch.com
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 9 Feb 2012 15:35:29 +0000
> From: Sarah Dysart <sdysart <@t> mirnarx.com>
> Subject: [Histonet] QIHC
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD <@t> BL2PRD0710MB363.namprd07.prod.outlook.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hey ya'll. I have been doing IHC for 10 years or so now. Do you think
> The QIHC exam would be much of a challenge if I went and tried to do it
> without much studying. I have never used automation to do IHC and have
> always had to troubleshoot my by hand stains myself. If you do think
> something would be worth going over what would you suggest?
> Thanks
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 9 Feb 2012 10:41:03 -0500
> From: Walter Benton <wbenton <@t> cua.md>
> Subject: [Histonet] RE: QIHC
> To: Sarah Dysart <sdysart <@t> mirnarx.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0B8979A204680A42B93A52B486088CD92B6A285E12 <@t> CUAEXH1.GCU-MD.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Make sure you are fimilar with ISH and IF techniques. I recall that being
> part of the test. Review the criteria on the ASCP website to see what they
> recommend as a study list.
>
> Walter Benton HT(ASCP)QIHC
> Histology Supervisor
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 126
> (All Deliveries to Suite 127)
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> wbenton <@t> cua.md
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
> [sdysart <@t> mirnarx.com]
> Sent: Thursday, February 09, 2012 10:35 AM
> To: histonet
> Subject: [Histonet] QIHC
>
> Hey ya'll. I have been doing IHC for 10 years or so now. Do you think
> The QIHC exam would be much of a challenge if I went and tried to do it
> without much studying. I have never used automation to do IHC and have
> always had to troubleshoot my by hand stains myself. If you do think
> something would be worth going over what would you suggest?
> Thanks
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> CONFIDENTIALITY NOTICE: The information contained in this electronic
> message is intended solely for the personal and confidential use of the
> designated recipient(s) named above and may contain information that is
> protected from disclosure under applicable law. If you are not the
> intended recipient, or the employee or agent responsible for delivering it
> to the intended recipient, you are hereby notified that any dissemination,
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> you have received this transmission in error, please notify the
> transmitting person/department immediately by email or telephone (410)
> 581-5881 and delete the message without making a copy.
>
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 9 Feb 2012 15:45:19 +0000
> From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
> Subject: [Histonet] exit strategy for staff
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <7722595275A4DD4FA225B92CDBF174A101A4F76957D8 <@t> EXC-MBX3.cfs.le.ac.uk>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone have a formalised exit strategy document for when somebody
> leaves an organisation, thinking about individuals either discarding or
> passing on specimens etc which they have been concerned with when they
> leave, and what they do with lab coats, lab books or security tags etc
> when they leave, if you get my drift?, many thanks.
>
> Richard Edwards
>
> University of Leicester
>
> U.K.
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 9 Feb 2012 11:16:59 -0500
> From: "Freeman, Carol" <Carol.Freeman <@t> utoledo.edu>
> Subject: [Histonet] (no subject)
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D8839F381A97B045BA5A607F5EEDF311018B5378 <@t> MSG02CV00.utad.utoledo.edu>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Just curious how people are handling their negative control slides for
> clinical settings. Thank you in advance for any responses.
>
>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 9 Feb 2012 09:16:43 -0800 (PST)
> From: angela smith <we3smitty <@t> yahoo.com>
> Subject: [Histonet] Flammable / explosion proof refrigerator
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <1328807803.38452.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> What is the criteria for volume and NFPA rating of a reagent for a
> flammable / explosion proof refrigertor?
>
> I know many of us do not have the necessary refrigerator, but I would
> assume there should be some kind of criteria or "rule" to help justify
> getting an equipped refrigerator for flammable refrigerated reagents.
>
> Please let me know some facts!
> Thanks
> Angela
>
> ------------------------------
>
> Message: 11
> Date: Thu, 9 Feb 2012 09:23:03 -0800
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: RE: [Histonet] Flammable / explosion proof refrigerator
> To: "'angela smith'" <we3smitty <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <8D7C2D242DBD45498006B21122072BF89F5EE7A9 <@t> MCINFRWEM003.ucsfmedicalcenter.org>
>
> Content-Type: text/plain; charset=iso-8859-1
>
> Angela, the only "requirement" I know of is your need to store solvents
> that could ignite if the thermostat switch sparks. We have one for special
> stains. We also have one that has one bottle of acetone in it. The vapor
> from that could ignite.
>
> They aren't that much more expensive than a regular refrigerator. They
> just put the thermostat switch on the outside rather than the inside.
>
> Tim Morken
> Supervisor, Histology, IPOX
> UCSF Medical Center
> San Francisco, CA, USA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of angela
> smith
> Sent: Thursday, February 09, 2012 9:17 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Flammable / explosion proof refrigerator
>
> What is the criteria for volume and NFPA rating of a reagent for a
> flammable / explosion proof refrigertor?
>
> I know many of us do not have the necessary refrigerator, but I would
> assume there should be some kind of criteria or "rule" to help justify
> getting an equipped refrigerator for flammable refrigerated reagents.
>
> Please let me know some facts!
> Thanks
> Angela
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 99, Issue 11
> ****************************************
>
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