[Histonet] RE: cryostats decontamination

Thu Feb 9 15:05:53 CST 2012

Seems like overkill to us, too, especially since we have to scrub ours down between each patient (we are sectioning tissue that goes to Molecular for DNA/RNA isolation after us) and had only been taking them completely down quarterly, never having more than 2 of our 5 units down at any time. Our cryostats look showroom new even in mid-shift. 
Now, we'll take them down on Friday afternoon, clean them on Monday morning and then wait for them to cool down enough to be able to work.

Barb Newton
Morphology Core Lab/TCGA Project
Nationwide Children's Hospital
614-355-2915

-----Original Message-----
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Subject: Histonet Digest, Vol 99, Issue 11

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Today's Topics:

   1. Temporary Tech Agencies (Jennifer MacDonald)
   2. cryostats decontamination (anita dudley)
   3. Revco -80 Freezer Sliding Drawer Rack Reviews (Lucie Guernsey)
   4. EGFR ISH protocol for Ventana (Lanigan, Christopher)
   5. New Histology Positions - Northern IL - Retained Search
      (Matt Ward)
   6. QIHC (Sarah Dysart)
   7. RE: QIHC (Walter Benton)
   8. exit  strategy for staff (Edwards, Richard E.)
   9. (no subject) (Freeman, Carol)
  10. Flammable / explosion proof refrigerator (angela smith)
  11. RE: Flammable / explosion proof refrigerator (Morken, Timothy)

----------------------------------------------------------------------

Message: 1
Date: Wed, 8 Feb 2012 09:40:10 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] Temporary Tech Agencies
To: histonet <@t> lists.utsouthwestern.edu
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	<OF17BAC925.14142466-ON8825799E.0060F27D-8825799E.00611E87 <@t> mtsac.edu>
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Hi All,
I have a lab that is in need of temporary help for a maternity leave. Does anyone know of a good temporary agency?
Thanks,
Jennifer MacDonald

------------------------------

Message: 2
Date: Wed, 8 Feb 2012 16:49:29 -0600
From: anita dudley <azdudley <@t> hotmail.com>
Subject: [Histonet] cryostats decontamination
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU163-W55B7DEA6E8729D2249FC95D17A0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Is everyone taking their crostats down weekly??  just seems overkill to me

thanks,
anita dudley 		 	   		  

------------------------------

Message: 3
Date: Wed, 8 Feb 2012 15:07:09 -0800
From: Lucie Guernsey <lguernsey <@t> ucsd.edu>
Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews
To: histonet <@t> lists.utsouthwestern.edu
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	<CANQOiZ3D1wVb-aV5orfCJ2kE8MRAABdtNUZUaXgi8L5_1dmpKg <@t> mail.gmail.com>
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Hi all,

We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2" box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks?

Thanks for your input!
Lucie

Lucie Guernsey
UC San Diego
lguernsey <@t> ucsd.edu

------------------------------

Message: 4
Date: Thu, 9 Feb 2012 07:02:05 -0500
From: "Lanigan, Christopher" <lanigac <@t> ccf.org>
Subject: [Histonet] EGFR ISH protocol for Ventana
To: histonet <@t> lists.utsouthwestern.edu
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	<9DDD0F026DC71B41B79D8E439D7951A30BAB4791 <@t> cchsclexmb69.cc.ad.cchs.net>
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------Original message ----------------------------------------

Hi 

Can someone help me with the EGFR ISH protocol for Ventana?

Thanks!

Mei Zheng, HTL(ASCP) QIHC 

Clinical Supervisor 

Immunohistochemistry Lab. 

Department of Pathology 

Brigham and Women's Hospital 

Boston, MA 02115 

Lab Tel: 617 732-7790

------Reply ----------------------------------------

Hi Mei,

Sorry for the delay.

Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the Ventana Benchmark XT.  

First, the software that you will need to have installed is called "XT Dual Color Open Probe".  This protocol will use the UltraView Silver ISH DNP Detection for EGFR and UltraView Red ISH DIG Detection for Chromosome 7.  The counterstain is Hematoxylin II with Bluing Reagent.

Before I get to the protocol, I'll fill you in on the EGFR and Chromosome 7 probes.  They do NOT arrive in a dispenser.  They each arrive in pre-diluted vials, so you will need to fill a "user-fillable dispenser".  The pre-diluted vials are mixed together into one user-fillable dispenser to create a "cocktail".  

Don't forget you will also need a "button kit" for "ISH PROBE" because the user-fillable dispenser will not have the proper commercial bar code.

One more thing, UltraView Red ISH DIG Detection cannot be dehydrated in alcohol.  I usually dry the slides at 65C for an hour before I coverslip. 

Finally, the following is a successful protocol summary.  

1 Deparaffinization [Selected]

2 Extended [Selected]

3 Warmup Slide to [72 Deg C], and Incubate for 4 Minutes ( Deparaffinization )

4 Cell Conditioning [Selected]

5 CC2 [Selected]

6 CC2 Cycle 1 [Selected]

7 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Cycle 1 )

8 CC2 Cycle 2 [Selected]

9 Incubate for [12 Minutes] ( Cycle 2 )

10 CC2 Cycle 3 [Selected]

11 Incubate for [12 Minutes] ( Cycle 3 )

12 Enzyme [Selected]

13 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), Apply Coverslip, and Incubate for [32 Minutes]

14 Probe Auto Dispense [Selected]

15 Apply One Drop of [ISH Probe 3] ( ISH Probe ), No Coverslip and Incubate for 8 Minutes

16 Denature [Selected]

17 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Denaturation )

18 Hybridization [Selected]

19 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization )

20 Incubate for [6 Hours] ( Hybridization )

21 Stringency Washes [Selected]

22 Stringency Wash #1 [Selected]

23 Warmup Slide to [72 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 )

24 Stringency Wash #2 [Selected]

25 Incubate for [8 Minutes] ( Stringency Wash #2 )

26 Stringency Wash #3 [Selected]

27 Incubate for [8 Minutes] ( Stringency Wash #3 )

28 Silver Detection [Selected]

29 Apply One Drop of SIL ISH DNP RAB, Apply Coverslip, and Incubate for
[20 Minutes]

30 Apply One Drop of SIL ISH DNP CHRC, and Incubate for [4 Minutes]

31 Red Detection [Selected]

32 Apply One Drop of RED ISH DIG MAB, Apply Coverslip, and Incubate for
[20 Minutes]

33 Apply One Drop of RED ISH DIG FR, and Incubate for [4 Minutes]

34 Counterstain [Selected]

35 Apply One Drop of [HEMATOXYLIN II] ( Counterstain ), Apply Coverslip, and Incubate for [8 Minutes]

36 Post Counterstain [Selected]

37 Apply One Drop of [BLUING REAGENT] ( Post Counterstain ), Apply Coverslip, and Incubate for [4 Minutes]

38 Post Run LCS Application [Selected]

Christopher Lanigan

Research Technologist

Molecular Pathology

Cleveland Clinic Foundation

9500 Euclid Avenue L3-127

Cleveland, OH 44195

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Message: 5
Date: Thu, 9 Feb 2012 10:00:59 -0500
From: Matt Ward <mw <@t> personifysearch.com>
Subject: [Histonet] New Histology Positions - Northern IL - Retained
	Search
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8b2c63aac6f85a2c063ac069a23a1e27 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Good Morning,

Personify has had 2 new permanent Histology opportunities become available in the Northern IL area. The positions offer competitive packages and the opportunity for career growth.

Please contact me directly at mw <@t> personifysearch to learn more.

*The Company:*

Well-established provider of consumables and medical device accessories for clinical histology and research laboratories.  The facility works closely with our UK, German and Australian facilities in the development, manufacture and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products.

This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries.

*The Opportunities:*

(1)    QA Histologist: The company currently has an opening for a Quality
Assurance Histologist to be based in Richmond IL.

(2)    Histologist: The company currently has an opening for a Histologist
working on the R&D team to be based in Richmond IL.

Salary: Based on Experience

Other: Full benefits - 401k program/matching

*Education and Experience Required:*

- Experience with tissue grossing, tissue processing and embedding
- Experience with sectioning paraffin embedded tissue as well as frozen tissue
- Experience performing routine stains (H and E) as well as special stains
- Experience with formulation and production of routine laboratory reagents and solutions
- Experience performing and documenting routine laboratory procedures
- Familiarity with compliance requirements in the medical device industry
- Proficiency in basic computer skills and with software applications such as Microsoft Office

Have a great day!

Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com

------------------------------

Message: 6
Date: Thu, 9 Feb 2012 15:35:29 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: [Histonet] QIHC
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD <@t> BL2PRD0710MB363.namprd07.prod.outlook.com>

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Hey ya'll.  I have been doing IHC for 10 years or so now.  Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying.  I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself.  If you do think something would be worth going over what would you suggest?
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

------------------------------

Message: 7
Date: Thu, 9 Feb 2012 10:41:03 -0500
From: Walter Benton <wbenton <@t> cua.md>
Subject: [Histonet] RE: QIHC
To: Sarah Dysart <sdysart <@t> mirnarx.com>, histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<0B8979A204680A42B93A52B486088CD92B6A285E12 <@t> CUAEXH1.GCU-MD.local>
Content-Type: text/plain; charset="us-ascii"

Make sure you are fimilar with ISH and IF techniques. I recall that being part of the test. Review the criteria on the ASCP website to see what they recommend as a study list.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton <@t> cua.md
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [sdysart <@t> mirnarx.com]
Sent: Thursday, February 09, 2012 10:35 AM
To: histonet
Subject: [Histonet] QIHC

Hey ya'll.  I have been doing IHC for 10 years or so now.  Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying.  I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself.  If you do think something would be worth going over what would you suggest?
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

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Message: 8
Date: Thu, 9 Feb 2012 15:45:19 +0000
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] exit  strategy for staff
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<7722595275A4DD4FA225B92CDBF174A101A4F76957D8 <@t> EXC-MBX3.cfs.le.ac.uk>
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Does anyone have a formalised exit strategy document for when somebody leaves an organisation, thinking about individuals either discarding or passing on specimens etc which they have been concerned with when they leave, and what they do with lab coats, lab books or security tags etc  when they leave, if you get my drift?, many thanks.

                       Richard  Edwards

                              University of Leicester

                                        U.K.

------------------------------

Message: 9
Date: Thu, 9 Feb 2012 11:16:59 -0500
From: "Freeman, Carol" <Carol.Freeman <@t> utoledo.edu>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<D8839F381A97B045BA5A607F5EEDF311018B5378 <@t> MSG02CV00.utad.utoledo.edu>
Content-Type: text/plain; charset="us-ascii"

Just curious how people are handling their negative control slides for clinical settings.  Thank you in advance for any responses.

------------------------------

Message: 10
Date: Thu, 9 Feb 2012 09:16:43 -0800 (PST)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: [Histonet] Flammable / explosion proof refrigerator
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<1328807803.38452.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor?
?
I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or "rule" to help justify getting an equipped refrigerator? for flammable refrigerated reagents.
?
Please let me know some facts!
Thanks
Angela

------------------------------

Message: 11
Date: Thu, 9 Feb 2012 09:23:03 -0800
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Flammable / explosion proof refrigerator
To: "'angela smith'" <we3smitty <@t> yahoo.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<8D7C2D242DBD45498006B21122072BF89F5EE7A9 <@t> MCINFRWEM003.ucsfmedicalcenter.org>

Content-Type: text/plain; charset=iso-8859-1

Angela, the only "requirement" I know of is your need to store solvents that could ignite if the thermostat switch sparks. We have one for special stains. We also have one that has one bottle of acetone in it. The vapor from that could ignite. 

They aren't that much more expensive than a regular refrigerator. They just put the thermostat switch on the outside rather than the inside. 

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of angela smith
Sent: Thursday, February 09, 2012 9:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Flammable / explosion proof refrigerator

What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor?
?
I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or "rule" to help justify getting an equipped refrigerator? for flammable refrigerated reagents.
?
Please let me know some facts!
Thanks
Angela
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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