[Histonet] Re: Histonet Digest, Vol 99, Issue 3
Madeleine Huey
madeleinehuey <@t> gmail.com
Sat Feb 4 22:53:14 CST 2012
Kathryn Stoll,
Did you freeze & thaw your -20c antibody? The freeze & thaw can cause
antibody degradation (ie. start with 1:100 to 1:20 with few thawing).
Have you consider switching storage antibodies from -20c to 4c?
Innovex (Berkeley, Ca) sell antibody diluent for this purpose.
Madeleine Huey, HT (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Thu, Feb 2, 2012 at 3:15 PM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. Topoisomerase I (Stoll, Kathryn)
> 2. EGFR ISH Ventana (Zheng, Mei)
> 3. reagents without expiration dates (Stoll, Kathryn)
> 4. RE: reagents without expiration dates (Goins, Tresa)
> 5. RE: reagents without expiration dates (WILLIAM DESALVO)
> 6. Re: slides for frozens (Sherwood, Margaret)
> 7. Re: slides for frozens (Rene J Buesa)
> 8. RE: slides for frozens (Sherwood, Margaret)
> 9. RE: slides for frozens (Rene J Buesa)
> 10. Re: reagents without expiration dates (Emily Sours)
> 11. histotech needed (Horn, Hazel V)
> 12. Re: reagents without expiration dates (Rene J Buesa)
> 13. Re: RE: Interviewing Histotechs... (Joe Nocito)
> 14. RE: reagents without expiration dates (Goins, Tresa)
> 15. RE: reagents without expiration dates (WILLIAM DESALVO)
> 16. RE: reagents without expiration dates (Jennifer MacDonald)
> 17. Re: AW: [Histonet] C4d IF on FFPE kidney (Tony Reilly)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 2 Feb 2012 12:16:35 -0600
> From: "Stoll, Kathryn" <kstoll <@t> mcw.edu>
> Subject: [Histonet] Topoisomerase I
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <110E7925E2B91945A9B79EDFD0DC2B34EB103BAD63 <@t> MCWMBX2.mcwcorp.net>
> Content-Type: text/plain; charset="us-ascii"
>
> I am using TOP1 that originally worked at 1:100 dilution. I am now using at 1:20.
> Any ideas why the staining has decreased so much? I have it stored in -20 in aliquots.
>
> Or has someone found a TOP1 that works for them.
>
> It is too expensive to run at 1:20. Thanks,
>
> Kathryn Stoll, HT(ASCP)
> Depatment of Pathology
> Medical College of Wisconsin
> 9200 W Wisconsin Ave
> Milwaukee WI 53226
> 414.805.1525
> kstoll <@t> mcw.edu
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 2 Feb 2012 13:51:10 -0500
> From: "Zheng, Mei" <MZHENG <@t> PARTNERS.ORG>
> Subject: [Histonet] EGFR ISH Ventana
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <7C58021620332F438692F2B4EFF5782703C56A3A <@t> PHSXMB26.partners.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi
>
> Can someone help me with the EGFR ISH protocol for Ventana?
>
> Thanks!
>
> Mei Zheng, HTL(ASCP) QIHC
> Clinical Supervisor
> Immunohistochemistry Lab.
> Department of Pathology
> Brigham and Women's Hospital
> Boston, MA 02115
> Lab Tel: 617 732-7790
>
>
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 2 Feb 2012 12:56:58 -0600
> From: "Stoll, Kathryn" <kstoll <@t> mcw.edu>
> Subject: [Histonet] reagents without expiration dates
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00 <@t> MCWMBX2.mcwcorp.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date?
> CAP checklist ANP 21382
>
> Thanks,
> Kathryn Stoll, HT(ASCP)
> Depatment of Pathology
> Medical College of Wisconsin
> 9200 W Wisconsin Ave
> Milwaukee WI 53226
> 414.805.1525
> kstoll <@t> mcw.edu
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 2 Feb 2012 19:04:03 +0000
> From: "Goins, Tresa" <TGoins <@t> mt.gov>
> Subject: [Histonet] RE: reagents without expiration dates
> To: "Stoll, Kathryn" <kstoll <@t> mcw.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CA4DF32ED505D94BB55E95487D8E98411F1D65BB <@t> DOAISD5205.state.mt.ads>
> Content-Type: text/plain; charset="us-ascii"
>
> A general rule that I am familiar with is a maximum shelf-life of 1 year, basically due to repetitive access leading to eventual contamination.
>
>
> Tresa Goins
> Veterinary Diagnostic Lab
> South 19th and Lincoln
> Bozeman, MT 59718
> 406-994-6353 - phone
> 406-994-6344 - fax
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stoll, Kathryn
> Sent: Thursday, February 02, 2012 11:57 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] reagents without expiration dates
>
> Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date?
> CAP checklist ANP 21382
>
> Thanks,
> Kathryn Stoll, HT(ASCP)
> Depatment of Pathology
> Medical College of Wisconsin
> 9200 W Wisconsin Ave
> Milwaukee WI 53226
> 414.805.1525
> kstoll <@t> mcw.edu
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 2 Feb 2012 12:07:31 -0700
> From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Subject: RE: [Histonet] reagents without expiration dates
> To: <kstoll <@t> mcw.edu>, histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY151-W26D6ED33518F5D51645F4791700 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Per SOP, we relabel, list date of receipt, test for quality and then apply a 12 month expiration date. We re-test after 12 months and continue to use, with 12 month dating, as long as the reagent meets quality standards set in the SOP.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>> From: kstoll <@t> mcw.edu
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Thu, 2 Feb 2012 12:56:58 -0600
>> Subject: [Histonet] reagents without expiration dates
>>
>> Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date?
>> CAP checklist ANP 21382
>>
>> Thanks,
>> Kathryn Stoll, HT(ASCP)
>> Depatment of Pathology
>> Medical College of Wisconsin
>> 9200 W Wisconsin Ave
>> Milwaukee WI 53226
>> 414.805.1525
>> kstoll <@t> mcw.edu
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 2 Feb 2012 14:46:40 -0500
> From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: Re: [Histonet] slides for frozens
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCD <@t> PHSXMB30.partners.org>
> Content-Type: text/plain; charset="us-ascii"
>
> To all:
>
> We have been using the same slides for paraffins and frozens. We have no
> trouble with paraffin-embedded tissue adhering to the slides through the whole
> staining procedure. But our frozens (even 5um sections)have been falling off
> during manual staining. The slides are (+) charged. We used to use slides from
> Fisher, but they are so expensive, we changed to an independent vendor (half the
> price).
>
> Our procedure for frozen H&Es has recently changed:
> 1. Fix in 95% ETOH 1 min.
> 2. Hematoxylin 5 min.
> 3. Acetic acid H2O 2 quick dips
> 4. Wash-running H2O 10 seconds (10 dips)
> 5. Bluing reagent 10 dips
> 6. Wash-running H2O 10 seconds (or 10 dips)
> 7. Eosin 5 seconds
> 8. Dehydrate-95% ETOH 10 dips each
> 9. Dehydrate-100%ETOH 10 dips each
> 10.CitriSolv 10 dips each
> 11.Coverslip
>
> The tissue seems to fall off in the bluing reagent (we order from Mossberg).
>
> Can anyone help us? Suggest anything--what slides you are using? We have had
> the same problem with special stains (i.e. Oil Red O for fat on frozens).
>
> Thanks!
> Peggy
>
> P.S. I use the same slides for my 1um plastic embedding which I dip in water
> rinses and have no problem with the tissue falling off.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood <@t> partners.org
>
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 2 Feb 2012 11:56:51 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] slides for frozens
> To: histonet <histonet <@t> lists.utsouthwestern.edu>, MargaretSherwood
> <MSHERWOOD <@t> PARTNERS.ORG>
> Message-ID:
> <1328212611.97248.YahooMailClassic <@t> web162102.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> There is a known saying that goes: "You get what you pay for".
> René J.
>
> --- On Thu, 2/2/12, Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG> wrote:
>
>
> From: Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: Re: [Histonet] slides for frozens
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, February 2, 2012, 2:46 PM
>
>
> To all:
>
> We have been using the same slides for paraffins and frozens. We have no
> trouble with paraffin-embedded tissue adhering to the slides through the whole
> staining procedure. But our frozens (even 5um sections)have been falling off
> during manual staining. The slides are (+) charged. We used to use slides from
> Fisher, but they are so expensive, we changed to an independent vendor (half the
> price).
>
> Our procedure for frozen H&Es has recently changed:
> 1. Fix in 95% ETOH 1 min.
> 2. Hematoxylin 5 min.
> 3. Acetic acid H2O 2 quick dips
> 4. Wash-running H2O 10 seconds (10 dips)
> 5. Bluing reagent 10 dips
> 6. Wash-running H2O 10 seconds (or 10 dips)
> 7. Eosin 5 seconds
> 8. Dehydrate-95% ETOH 10 dips each
> 9. Dehydrate-100%ETOH 10 dips each
> 10.CitriSolv 10 dips each
> 11.Coverslip
>
> The tissue seems to fall off in the bluing reagent (we order from Mossberg).
>
> Can anyone help us? Suggest anything--what slides you are using? We have had
> the same problem with special stains (i.e. Oil Red O for fat on frozens).
>
> Thanks!
> Peggy
>
> P.S. I use the same slides for my 1um plastic embedding which I dip in water
> rinses and have no problem with the tissue falling off.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood <@t> partners.org
>
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 2 Feb 2012 15:03:35 -0500
> From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: RE: [Histonet] slides for frozens
> To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCE <@t> PHSXMB30.partners.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> That may be true, but I don't recall having this problem with our previous
> protocol (which was longer and included a fix in alcoholic formalin). I think
> we will try the "old" method and see what happens.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood <@t> partners.org
>
>
>
> ________________________________
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, February 02, 2012 2:57 PM
> To: histonet; Sherwood, Margaret
> Subject: Re: [Histonet] slides for frozens
>
>
> There is a known saying that goes: "You get what you pay for".
> René J.
>
> --- On Thu, 2/2/12, Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG> wrote:
>
>
>
> From: Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: Re: [Histonet] slides for frozens
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, February 2, 2012, 2:46 PM
>
>
> To all:
>
> We have been using the same slides for paraffins and frozens. We have
> no
> trouble with paraffin-embedded tissue adhering to the slides through the
> whole
> staining procedure. But our frozens (even 5um sections)have been
> falling off
> during manual staining. The slides are (+) charged. We used to use
> slides from
> Fisher, but they are so expensive, we changed to an independent vendor
> (half the
> price).
>
> Our procedure for frozen H&Es has recently changed:
> 1. Fix in 95% ETOH 1 min.
> 2. Hematoxylin 5 min.
> 3. Acetic acid H2O 2 quick dips
> 4. Wash-running H2O 10 seconds (10 dips)
> 5. Bluing reagent 10 dips
> 6. Wash-running H2O 10 seconds (or 10 dips)
> 7. Eosin 5 seconds
> 8. Dehydrate-95% ETOH 10 dips each
> 9. Dehydrate-100%ETOH 10 dips each
> 10.CitriSolv 10 dips each
> 11.Coverslip
>
> The tissue seems to fall off in the bluing reagent (we order from
> Mossberg).
>
> Can anyone help us? Suggest anything--what slides you are using? We
> have had
> the same problem with special stains (i.e. Oil Red O for fat on
> frozens).
>
> Thanks!
> Peggy
>
> P.S. I use the same slides for my 1um plastic embedding which I dip in
> water
> rinses and have no problem with the tissue falling off.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood <@t> partners.org
> <http://us.mc1621.mail.yahoo.com/mc/compose?to=msherwood@partners.org>
>
>
>
> The information in this e-mail is intended only for the person to whom
> it is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you
> in error
> but does not contain patient information, please contact the sender and
> properly
> dispose of the e-mail.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> <http://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu
>>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 2 Feb 2012 12:08:02 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: RE: [Histonet] slides for frozens
> To: MargaretSherwood <MSHERWOOD <@t> PARTNERS.ORG>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <1328213282.15651.YahooMailClassic <@t> web162102.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> That could be the solution. The problem is that you changed 2 things: the protocol and the slides and now you do not know which is "the culprit". Returning to the old protocols is a first step to trying to solve the present situation and, as you can imagine, if the old protocol does not solve the problem, the slides may be the cause.
> René J.
>
> --- On Thu, 2/2/12, Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG> wrote:
>
>
> From: Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: RE: [Histonet] slides for frozens
> To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, February 2, 2012, 3:03 PM
>
>
> That may be true, but I don't recall having this problem with our previous
> protocol (which was longer and included a fix in alcoholic formalin). I think
> we will try the "old" method and see what happens.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood <@t> partners.org
>
>
>
> ________________________________
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, February 02, 2012 2:57 PM
> To: histonet; Sherwood, Margaret
> Subject: Re: [Histonet] slides for frozens
>
>
> There is a known saying that goes: "You get what you pay for".
> René J.
>
> --- On Thu, 2/2/12, Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG> wrote:
>
>
>
> From: Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: Re: [Histonet] slides for frozens
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Date: Thursday, February 2, 2012, 2:46 PM
>
>
> To all:
>
> We have been using the same slides for paraffins and frozens. We have
> no
> trouble with paraffin-embedded tissue adhering to the slides through the
> whole
> staining procedure. But our frozens (even 5um sections)have been
> falling off
> during manual staining. The slides are (+) charged. We used to use
> slides from
> Fisher, but they are so expensive, we changed to an independent vendor
> (half the
> price).
>
> Our procedure for frozen H&Es has recently changed:
> 1. Fix in 95% ETOH 1 min.
> 2. Hematoxylin 5 min.
> 3. Acetic acid H2O 2 quick dips
> 4. Wash-running H2O 10 seconds (10 dips)
> 5. Bluing reagent 10 dips
> 6. Wash-running H2O 10 seconds (or 10 dips)
> 7. Eosin 5 seconds
> 8. Dehydrate-95% ETOH 10 dips each
> 9. Dehydrate-100%ETOH 10 dips each
> 10.CitriSolv 10 dips each
> 11.Coverslip
>
> The tissue seems to fall off in the bluing reagent (we order from
> Mossberg).
>
> Can anyone help us? Suggest anything--what slides you are using? We
> have had
> the same problem with special stains (i.e. Oil Red O for fat on
> frozens).
>
> Thanks!
> Peggy
>
> P.S. I use the same slides for my 1um plastic embedding which I dip in
> water
> rinses and have no problem with the tissue falling off.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood <@t> partners.org
> <http://us.mc1621.mail.yahoo.com/mc/compose?to=msherwood@partners.org>
>
>
>
> The information in this e-mail is intended only for the person to whom
> it is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you
> in error
> but does not contain patient information, please contact the sender and
> properly
> dispose of the e-mail.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> <http://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu
>>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 2 Feb 2012 15:33:10 -0500
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Subject: Re: [Histonet] reagents without expiration dates
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAP=XX1wH3-nb3UWnMTRFju111j+vgvivyasH69BDGwhHJL+qDg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> I've always wanted to have a contest to see who had the oldest reagents.
> My lab once had something that was 20 years old.
>
> Emily
>
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
>
>
>
> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>wrote:
>
>>
>> Per SOP, we relabel, list date of receipt, test for quality and then apply
>> a 12 month expiration date. We re-test after 12 months and continue to use,
>> with 12 month dating, as long as the reagent meets quality standards set in
>> the SOP.
>>
>> William DeSalvo, B.S., HTL(ASCP)
>>
>>
>>
>> > From: kstoll <@t> mcw.edu
>> > To: histonet <@t> lists.utsouthwestern.edu
>> > Date: Thu, 2 Feb 2012 12:56:58 -0600
>> > Subject: [Histonet] reagents without expiration dates
>> >
>> > Could anyone share a policy to deal with regents that do not have a
>> manufacturer's expiration date?
>> > CAP checklist ANP 21382
>> >
>> > Thanks,
>> > Kathryn Stoll, HT(ASCP)
>> > Depatment of Pathology
>> > Medical College of Wisconsin
>> > 9200 W Wisconsin Ave
>> > Milwaukee WI 53226
>> > 414.805.1525
>> > kstoll <@t> mcw.edu
>> >
>> > _______________________________________________
>> > Histonet mailing list
>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> ------------------------------
>
> Message: 11
> Date: Thu, 2 Feb 2012 15:11:12 -0600
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> Subject: [Histonet] histotech needed
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <25A4DE08332B19499904459F00AAACB719ACB9CBF2 <@t> EVS1.archildrens.org>
> Content-Type: text/plain; charset="ISO-8859-1"
>
> Arkansas Children's Hospital is looking for a registered histotech. This is a dayshift, Monday-Friday position with rotating holidays on call. Our lab performs routine H&E's, special stains by hand and we have a Bond III immunostainer. We process approximately 6000 surgicals a year. ACH has excellent benefits. Little Rock is nice small city in which to live. We have many area attractions and if you like the outdoor life you will love Arkansas. The position is posted as an MLT, histology lab. Apply online at:
> www.archildrens.org<http://www.archildrens.org>
>
>
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Autopsy/Histology/Transcription
> Arkansas Children's Hospital
> 1 Children's Way Slot 820
> Little Rock, AR 72202
>
> phone 501.364.4240
> fax 501.364.3155
>
> visit us on the web at: www.archildrens.org
>
> ******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
> The information contained in this message may be privileged and confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please notify
> us immediately by replying to the message and deleting it from your computer.
> Thank you.
>
>
> ------------------------------
>
> Message: 12
> Date: Thu, 2 Feb 2012 13:14:53 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] reagents without expiration dates
> To: histonet <@t> lists.utsouthwestern.edu, Emily Sours
> <talulahgosh <@t> gmail.com>
> Message-ID:
> <1328217293.25010.YahooMailClassic <@t> web162101.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> Ha.Ha,Ha!!!!!!!!!!!
> I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression).
> Try to beat that!.
> René J.
>
> --- On Thu, 2/2/12, Emily Sours <talulahgosh <@t> gmail.com> wrote:
>
>
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Subject: Re: [Histonet] reagents without expiration dates
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, February 2, 2012, 3:33 PM
>
>
> I've always wanted to have a contest to see who had the oldest reagents.
> My lab once had something that was 20 years old.
>
> Emily
>
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
>
>
>
> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>wrote:
>
>>
>> Per SOP, we relabel, list date of receipt, test for quality and then apply
>> a 12 month expiration date. We re-test after 12 months and continue to use,
>> with 12 month dating, as long as the reagent meets quality standards set in
>> the SOP.
>>
>> William DeSalvo, B.S., HTL(ASCP)
>>
>>
>>
>> > From: kstoll <@t> mcw.edu
>> > To: histonet <@t> lists.utsouthwestern.edu
>> > Date: Thu, 2 Feb 2012 12:56:58 -0600
>> > Subject: [Histonet] reagents without expiration dates
>> >
>> > Could anyone share a policy to deal with regents that do not have a
>> manufacturer's expiration date?
>> > CAP checklist ANP 21382
>> >
>> > Thanks,
>> > Kathryn Stoll, HT(ASCP)
>> > Depatment of Pathology
>> > Medical College of Wisconsin
>> > 9200 W Wisconsin Ave
>> > Milwaukee WI 53226
>> > 414.805.1525
>> > kstoll <@t> mcw.edu
>> >
>> > _______________________________________________
>> > Histonet mailing list
>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>Â _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 13
> Date: Thu, 2 Feb 2012 16:41:07 -0600
> From: "Joe Nocito" <jnocito <@t> satx.rr.com>
> Subject: Re: [Histonet] RE: Interviewing Histotechs...
> To: "Kim Donadio" <one_angel_secret <@t> yahoo.com>, "Jerry Ricks"
> <rosenfeldtek <@t> hotmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8E6FB415E5AB409C90E5494BC877D04F <@t> JoePC>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> yeah, I never did like that the "a monkey can do it" crap. A pathologist
> told me that he could teach a monkey to gross. When the grossing tech messed
> up a case, I was called in and got jumped on. He really didn't appreciate it
> when I told him he better get that monkey trained quick.
>
> JTT
>
> ----- Original Message -----
> From: "Kim Donadio" <one_angel_secret <@t> yahoo.com>
> To: "Jerry Ricks" <rosenfeldtek <@t> hotmail.com>
> Cc: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, January 31, 2012 12:46 PM
> Subject: Re: [Histonet] RE: Interviewing Histotechs...
>
>
> Your comment about a monkey hits a nerve. There is a misconception I think
> in our field that yes any monkey off the street can do our job. Well they
> can't. It takes a good amount of knowledge to understand tissues, stains,
> chemical reactions and yes you will need to have some amount of hand eye
> coordination Skill .
>
> It's the monkey theory that has histotechs jumping up and down into these
> quick almost no hands on programs so they can get a good paying job with not
> much invested I'm afraid. Yes, I'm an expert at sticking my foot in my
> mouth. But if we as a group don't recognize why we are even having this
> debate about testing techs on the most basic of functions. Then I worry
> about the future of our profession And even healthcare because by god if you
> want monkeys , think monkeys , you will get monkeys!
>
> And darn it. I can't run and hide from this one can I lol
>
> Have a great week!
>
> Kim D
> Sent from my iPhone
>
> On Jan 31, 2012, at 1:13 PM, Jerry Ricks <rosenfeldtek <@t> hotmail.com> wrote:
>
>>
>>
>>
>>
>> Hi Toysha
>>
>> I think I'm just coming at it from "research mode" not clinical. Hands on
>> Histotechnology is a core part of our work, but just part, and it is
>> focused on animal models of cardiovascular disease. Depending on whether
>> the researcher is a postdoc or an undergrad they will have more or fewer
>> general lab skills including histo skills. I haven't met anyone yet who
>> did not need some training for embedding of brachiocephalic arteries of
>> mice.
>>
>> I doubt I would do well in a clinical lab. I've become accustomed to docs
>> saying "wow that's beautiful can you teach me how to do that?" I gather
>> in the clinical field it's more like "a monkey can learn how to section."
>> Maybe a good monkey could but I doubt it could work up an IHC with a new
>> antibody.
>>
>>
>> Jerry
>>
>>
>>> From: TNMayer <@t> mdanderson.org
>>> To: histonet <@t> lists.utsouthwestern.edu
>>> Date: Tue, 31 Jan 2012 10:38:58 -0600
>>> Subject: [Histonet] RE: Interviewing Histotechs...
>>>
>>> Jerry,
>>> I agree with you somewhat. I have met techs that misrepresented
>>> themselves and said that they could cut or embed, and knew how to operate
>>> the instruments, but could not produce quality work. You are right when
>>> you said that it is different for clinical vs. research. I have almost
>>> always worked clinical, and noticed that when working with research
>>> techs, they had a difficult time adjusting to clinical with the time
>>> frames and quality.
>>> When training new hires, depending on the position I am hiring for, I
>>> expect to train in the new workflow that they have learned, the new
>>> instrument they use, not the basic skills. I only expect to do that with
>>> a student. Fresh techs are expected to know how to get a section, not cut
>>> the plastic on the block, embed skin, and set up the h&e stainer. I
>>> should only have to go over and orient them on "our procedure" not teach
>>> the skill.
>>> I have worked various part-time jobs over the years and the first thing I
>>> ask is 'how many microns do you cut at here'? While 3-4 is the standard,
>>> some labs want everything at 3, or some at 4. I know how to cut, but
>>> like you it takes about 2 weeks to get used to the new instrument. That's
>>> fine, but I don't expect to have to teach the tech how to embed a skin or
>>> cut a kidney biopsy. Not for an experienced tech, unless they have never
>>> encountered it. That has to be made known during the interview.
>>> Yes, a cutting test is good, I have seen registered techs not make it
>>> past probation (90 days) because they could not cut. It would have saved
>>> the company time and MONEY if a test could have been given. Asking if
>>> they can cut a kidney biopsy, or embed a skin would be good as well. I
>>> can't go back and get more epithelia or ask for another pass through the
>>> kidney.
>>>
>>>
>>>
>>> Toysha N. Mayer, MBA, HT (ASCP)
>>> Instructor, Education Coordinator
>>> Program in Histotechnology
>>> School of Health Professions
>>> MD Anderson Cancer Center
>>> (713) 563-3481
>>> tnmayer <@t> mdanderson.org
>>>
>>>
>>>
>>>
>>>
>>>
>>> Message: 5
>>> Date: Mon, 30 Jan 2012 11:13:11 -0800
>>> From: Jerry Ricks <rosenfeldtek <@t> hotmail.com>
>>> Subject: [Histonet] Interviewing Histotechs...
>>> To: <histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID: <BAY161-W3593BCA5DAB2C7994A0D4CDB8D0 <@t> phx.gbl>
>>> Content-Type: text/plain; charset="iso-8859-1"
>>>
>>>
>>> I gather it is different in clinical labs than in research labs. In
>>> clinical labs there is an emphasis on quantity and speed. In research
>>> the emphasis is on doing good experiments. Our "patients" are almost
>>> always deceased or shortly about to be so there is no urgency of
>>> diagnosis factor. For us, "diagnosis" means making precise measurements
>>> else some scientists looking at an image and asking each other "what
>>> the?"
>>>
>>> Anyway I always assume that the person I am hiring is incompetent at
>>> histology and that they will need to be personally trained by me.
>>> Doesn't matter how much experience they have. And over 23 years that has
>>> turned out to be true. I've met exactly two people who didn't need much
>>> training. One was a former senior clinical lab manager. The other was
>>> a kid straight out of high school who happened to have a histology
>>> experience from high school and a decent histo portfolio. Yes, Mercer
>>> Island High School had a Histology program.
>>>
>>> No such thing as a tech who doesn't need to be trained and any tech
>>> trained by me will be up and running in a week or two. Why bother making
>>> them cut or stain anything during a darn interview. If they are smart
>>> and cooperative they will work out.
>>>
>>> If I ever go to a new lab with a new microtome, new protocols, I am
>>> pretty sure that I will be sort of incompetent for a week or two as well.
>>>
>>> Jerry Ricks
>>> Research Scientist
>>> University of Washington
>>> Department of Pathology
>>>
>>>
>>>
>>> histonet <@t> lists.utsouthwestern.edu
>>>> Date: Sun, 29 Jan 2012 13:12:09 -0500
>>>> From: rsrichmond <@t> gmail.com
>>>> To: histonet <@t> lists.utsouthwestern.edu
>>>> Subject: [Histonet] Re: interview....
>>>>
>>>> Ray Koelling asked me:
>>>>
>>>>>> If the Samurai Pathologist is out there reading still; any idea over
>>>>>> your career, about how many glass slides have you viewed under a
>>>>>> microscope since the first? Your replies are always top-notch,
>>>>>> entertaining and informative. And hope with each new job you don't
>>>>>> have to show someone you can pass a test of which slide shows normal
>>>>>> liver and which slide shows cirrhotic liver in your interview.<<
>>>>
>>>> I really have no idea how many slides. In a normal year I sign out
>>>> about 3,000 histology cases (remember I don't work full time)
>>>> averaging maybe 3 slides per case.
>>>>
>>>> Generally I've gotten jobs, both private clients and agency clients,
>>>> by recommendation. A number of years ago I was interviewed by a
>>>> four-pathologist hospital group who handed me a tray of 20 slides with
>>>> the necessary historical information, and was told that this was a set
>>>> the group had collected, including very straightforward cases, cases
>>>> with serious diagnostic pitfalls, and some cases they'd never been
>>>> able to make a diagnosis on. They tried to make it a test of judgment
>>>> rather than simple diagnostic skill. Told to take as much time as I
>>>> needed. I guess I passed - by coincidence, the entire group chanced to
>>>> break up very quickly, and an entirely different team took over.
>>>>
>>>> Bob Richmond
>>>> Samurai Pathologist
>>>> Knoxville TN
>>>>
>>> ************************************
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Thu, 2 Feb 2012 22:50:10 +0000
> From: "Goins, Tresa" <TGoins <@t> mt.gov>
> Subject: RE: [Histonet] reagents without expiration dates
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Emily Sours
> <talulahgosh <@t> gmail.com>
> Message-ID:
> <CA4DF32ED505D94BB55E95487D8E98411F1D663E <@t> DOAISD5205.state.mt.ads>
> Content-Type: text/plain; charset="utf-8"
>
> OK, I'll give it a try René. "Baker's Analyzed" Potassium Permanganate (5 lbs) with a "typed" label made with an actual "typewriter". Trying to verify age but I've wasted enough time already on fun stuff today.
>
>
>
> Tresa
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Thursday, February 02, 2012 2:15 PM
> To: histonet <@t> lists.utsouthwestern.edu; Emily Sours
> Subject: Re: [Histonet] reagents without expiration dates
>
>
>
> Ha.Ha,Ha!!!!!!!!!!!
>
> I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression).
>
> Try to beat that!.
>
> René J.
>
>
>
> --- On Thu, 2/2/12, Emily Sours <talulahgosh <@t> gmail.com<mailto:talulahgosh <@t> gmail.com>> wrote:
>
>
>
>
>
> From: Emily Sours <talulahgosh <@t> gmail.com<mailto:talulahgosh <@t> gmail.com>>
>
> Subject: Re: [Histonet] reagents without expiration dates
>
> To: histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
>
> Date: Thursday, February 2, 2012, 3:33 PM
>
>
>
>
>
> I've always wanted to have a contest to see who had the oldest reagents.
>
> My lab once had something that was 20 years old.
>
>
>
> Emily
>
>
>
> The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful.
>
> --Ron Swanson
>
>
>
>
>
>
>
> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com<mailto:wdesalvo.cac <@t> hotmail.com>>wrote:
>
>
>
>>
>
>> Per SOP, we relabel, list date of receipt, test for quality and then
>
>> apply a 12 month expiration date. We re-test after 12 months and
>
>> continue to use, with 12 month dating, as long as the reagent meets
>
>> quality standards set in the SOP.
>
>>
>
>> William DeSalvo, B.S., HTL(ASCP)
>
>>
>
>>
>
>>
>
>> > From: kstoll <@t> mcw.edu<mailto:kstoll <@t> mcw.edu>
>
>> > To: histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
>
>> > Date: Thu, 2 Feb 2012 12:56:58 -0600
>
>> > Subject: [Histonet] reagents without expiration dates
>
>> >
>
>> > Could anyone share a policy to deal with regents that do not have a
>
>> manufacturer's expiration date?
>
>> > CAP checklist ANP 21382
>
>> >
>
>> > Thanks,
>
>> > Kathryn Stoll, HT(ASCP)
>
>> > Depatment of Pathology
>
>> > Medical College of Wisconsin
>
>> > 9200 W Wisconsin Ave
>
>> > Milwaukee WI 53226
>
>> > 414.805.1525
>
>> > kstoll <@t> mcw.edu<mailto:kstoll <@t> mcw.edu>
>
>> >
>
>> > _______________________________________________
>
>> > Histonet mailing list
>
>> > Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
>
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>>
>
>> _______________________________________________
>
>> Histonet mailing list
>
>> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>>
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 2 Feb 2012 16:02:34 -0700
> From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Subject: RE: [Histonet] reagents without expiration dates
> To: <rjbuesa <@t> yahoo.com>, histonet <histonet <@t> lists.utsouthwestern.edu>,
> <talulahgosh <@t> gmail.com>
> Message-ID: <BAY151-W300C5CB054343D276314D691700 <@t> phx.gbl>
> Content-Type: text/plain; charset="Windows-1252"
>
>
> Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>> Date: Thu, 2 Feb 2012 13:14:53 -0800
>> From: rjbuesa <@t> yahoo.com
>> To: histonet <@t> lists.utsouthwestern.edu; talulahgosh <@t> gmail.com
>> Subject: Re: [Histonet] reagents without expiration dates
>> CC:
>>
>> Ha.Ha,Ha!!!!!!!!!!!
>> I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression).
>> Try to beat that!.
>> René J.
>>
>> --- On Thu, 2/2/12, Emily Sours <talulahgosh <@t> gmail.com> wrote:
>>
>>
>> From: Emily Sours <talulahgosh <@t> gmail.com>
>> Subject: Re: [Histonet] reagents without expiration dates
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Thursday, February 2, 2012, 3:33 PM
>>
>>
>> I've always wanted to have a contest to see who had the oldest reagents.
>> My lab once had something that was 20 years old.
>>
>> Emily
>>
>> The whole point of this country is if you want to eat garbage, balloon up
>> to 600 pounds and die of a heart attack at 43, you can! You are free to do
>> so. To me, that’s beautiful.
>> --Ron Swanson
>>
>>
>>
>> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>wrote:
>>
>> >
>> > Per SOP, we relabel, list date of receipt, test for quality and then apply
>> > a 12 month expiration date. We re-test after 12 months and continue to use,
>> > with 12 month dating, as long as the reagent meets quality standards set in
>> > the SOP.
>> >
>> > William DeSalvo, B.S., HTL(ASCP)
>> >
>> >
>> >
>> > > From: kstoll <@t> mcw.edu
>> > > To: histonet <@t> lists.utsouthwestern.edu
>> > > Date: Thu, 2 Feb 2012 12:56:58 -0600
>> > > Subject: [Histonet] reagents without expiration dates
>> > >
>> > > Could anyone share a policy to deal with regents that do not have a
>> > manufacturer's expiration date?
>> > > CAP checklist ANP 21382
>> > >
>> > > Thanks,
>> > > Kathryn Stoll, HT(ASCP)
>> > > Depatment of Pathology
>> > > Medical College of Wisconsin
>> > > 9200 W Wisconsin Ave
>> > > Milwaukee WI 53226
>> > > 414.805.1525
>> > > kstoll <@t> mcw.edu
>> > >
>> > > _______________________________________________
>> > > Histonet mailing list
>> > > Histonet <@t> lists.utsouthwestern.edu
>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
>> > _______________________________________________
>> > Histonet mailing list
>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 16
> Date: Thu, 2 Feb 2012 15:07:20 -0800
> From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Subject: RE: [Histonet] reagents without expiration dates
> To: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF28E83711.2058D44B-ON88257998.007EEBF2-88257998.007F041F <@t> mtsac.edu>
> Content-Type: text/plain; charset="UTF-8"
>
> I had a student that was working in a lab with old dry powders. One that
> I recall had a "date opened" of 1942.
>
>
>
>
> WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 02/02/2012 03:04 PM
>
> To
> <rjbuesa <@t> yahoo.com>, histonet <histonet <@t> lists.utsouthwestern.edu>,
> <talulahgosh <@t> gmail.com>
> cc
>
> Subject
> RE: [Histonet] reagents without expiration dates
>
>
>
>
>
>
>
> Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich
> and was visiting Charles Churukian's lab. He had a full wall of his lab
> with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had
> every lab chemical and reagent I knew and was very proud that he was a
> great Sigma customer. The labels were none that I had seen in 10 yrs
> working at Sigma, so I took a few containers down and read the
> manufacturing code. The oldest was sodium bisulfate, gallon container,
> manufactured in 1946. Most were 20-30 yrs old then and in large
> quantities. I have always thought to myself, with many more customers like
> Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still
> teaching the heavens everything they need to know about Histology and
> Staining. I don't know if that beats your stuff, but I bet the chemicals
> Chuck left behind are still in use.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>> Date: Thu, 2 Feb 2012 13:14:53 -0800
>> From: rjbuesa <@t> yahoo.com
>> To: histonet <@t> lists.utsouthwestern.edu; talulahgosh <@t> gmail.com
>> Subject: Re: [Histonet] reagents without expiration dates
>> CC:
>>
>> Ha.Ha,Ha!!!!!!!!!!!
>> I used to prepare staining solutions with some "Merck-Darmstad" anilines
> manufactured just after the "Great War", i.e. the FIRST World War (about
> 1925 before the World Great Depression).
>> Try to beat that!.
>> René J.
>>
>> --- On Thu, 2/2/12, Emily Sours <talulahgosh <@t> gmail.com> wrote:
>>
>>
>> From: Emily Sours <talulahgosh <@t> gmail.com>
>> Subject: Re: [Histonet] reagents without expiration dates
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Thursday, February 2, 2012, 3:33 PM
>>
>>
>> I've always wanted to have a contest to see who had the oldest reagents.
>> My lab once had something that was 20 years old.
>>
>> Emily
>>
>> The whole point of this country is if you want to eat garbage, balloon
> up
>> to 600 pounds and die of a heart attack at 43, you can! You are free to
> do
>> so. To me, that’s beautiful.
>> --Ron Swanson
>>
>>
>>
>> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO
> <wdesalvo.cac <@t> hotmail.com>wrote:
>>
>> >
>> > Per SOP, we relabel, list date of receipt, test for quality and then
> apply
>> > a 12 month expiration date. We re-test after 12 months and continue to
> use,
>> > with 12 month dating, as long as the reagent meets quality standards
> set in
>> > the SOP.
>> >
>> > William DeSalvo, B.S., HTL(ASCP)
>> >
>> >
>> >
>> > > From: kstoll <@t> mcw.edu
>> > > To: histonet <@t> lists.utsouthwestern.edu
>> > > Date: Thu, 2 Feb 2012 12:56:58 -0600
>> > > Subject: [Histonet] reagents without expiration dates
>> > >
>> > > Could anyone share a policy to deal with regents that do not have a
>> > manufacturer's expiration date?
>> > > CAP checklist ANP 21382
>> > >
>> > > Thanks,
>> > > Kathryn Stoll, HT(ASCP)
>> > > Depatment of Pathology
>> > > Medical College of Wisconsin
>> > > 9200 W Wisconsin Ave
>> > > Milwaukee WI 53226
>> > > 414.805.1525
>> > > kstoll <@t> mcw.edu
>> > >
>> > > _______________________________________________
>> > > Histonet mailing list
>> > > Histonet <@t> lists.utsouthwestern.edu
>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
>> > _______________________________________________
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>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
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>
> ------------------------------
>
> Message: 17
> Date: Fri, 03 Feb 2012 09:14:24 +1000
> From: "Tony Reilly" <Tony_Reilly <@t> health.qld.gov.au>
> Subject: Re: AW: [Histonet] C4d IF on FFPE kidney
> To: <gu.lang <@t> gmx.at>, "'Richard Cartun'" <Rcartun <@t> harthosp.org>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4F2BA56F.411C.0039.0 <@t> health.qld.gov.au>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi Gudren
>
> We have been doing both IF and IHC for C4d for quite a while. While we
> have found that the IF staining is superior to the IHC we are not
> unhappy with the IHC results. Our IHC results have improved since
> getting a Ventana Ultra which has allowed better control of the
> staining.
>
> regards
> Tony
>
>
>
>
>
> Tony Reilly B.App.Sc. , M.Sc.
> Chief Scientist, Anatomical Pathology
> Pathology Queensland-PA Laboratory
> _________________________________________________
> Clinical and Statewide Services Division| QueenslandHealth
>
> Level 1, Building 15,Princess Alexandra Hospital
> Ipswich Road,WOOLLOONGABBA Qld4102
> Ph: 07 3176 2412
> Mob: 0402 139411
> Fax: 07 3176 2930
> Email: tony_reilly <@t> health.qld.gov.au
> Web: www.health.qld.gov.au/qhcss/
>
>
>
>
>>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 2/3/2012 12:31 am >>>
> We have been performing C4d IHC on FFPE kidney for a couple of years.
> We
> receive only fixed samples.
> Yesterday my doctors came with this idea of IF - I still have to ask
> for the
> special reasons. But I have the suspicion, that they are not aware of
> the
> fact, that IF on frozen unfixed tissue is the usual way found in
> literature.
>
> There are some articles that deal with comparison of IF(frozen) and
> IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows
> additional staining in glomeruli.
>
> Perhaps someone told them, that IF is the golden standard and
> recommended.
>
> Gudrun
>
> -----Ursprüngliche Nachricht-----
> Von: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
> Gesendet: Mittwoch, 01. Februar 2012 21:21
> An: gu.lang <@t> gmx.at
> Betreff: Re: [Histonet] C4d IF on FFPE kidney
>
> Hi Gudrun:
>
> Is there reason why you want to use IF and not immunoperoxidase?
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 2/1/2012 3:17 PM >>>
> Hi!
>
> Can someone provide me a immunofluorescence protocol for C4d on
> formalin
> fixed human paraffin sections?
>
>
>
> thanks in advance
>
>
>
> Gudrun Lang
>
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> End of Histonet Digest, Vol 99, Issue 3
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