[Histonet] RE: Breast IHC testing

[Morken, Timothy]

Remember that you can validate your testing for longer fixation. Run parallel  ER/PR/Her2 testing with tissue fixed for various lengths of  times and look at the staining correlation between groups. Most likely your longer fixation won't be a problem.  If you do see a drop off with longer times you can probably adjust antigen retrieval time to alleviate that.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gale Limron
Sent: Friday, February 03, 2012 7:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Breast IHC testing

I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR  and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours.
Thank you!
Gale Limron CT,HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



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