From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Feb 1 07:56:48 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Feb 1 07:57:30 2012 Subject: [Histonet] RAC Medicare Audits - PAs In-Reply-To: <4F283EDD.7400.0077.1@harthosp.org> References: <4F283EDD.7400.0077.1@harthosp.org> Message-ID: I haven't heard that one. Do they mean present physically or present as in their office reading slides, but a phone call or page away? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Tuesday, January 31, 2012 7:19 PM To: Histonet Subject: [Histonet] RAC Medicare Audits - PAs Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From we3smitty <@t> yahoo.com Wed Feb 1 07:57:27 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Wed Feb 1 07:57:34 2012 Subject: [Histonet] Bleaching in the histo lab Message-ID: <1328104647.83036.YahooMailClassic@web125402.mail.ne1.yahoo.com> I have been told by our safety officer that it is standard practice too clean the lab at the end of the day with diluted bleach. I have noticed a chemical reaction (smell) when cleaning the main area of the lab. I have concerns that this is not a good practice due to chemical reactions as we use so many chemicals in histology. What do other people do?? Also I believe it is unsafe to use bleach with anything formalin related. Please let me know if you have a "standard" practice or mandated cleaning from your facility. Angela From dellav <@t> musc.edu Wed Feb 1 07:50:38 2012 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Feb 1 08:04:08 2012 Subject: [Histonet] Vendors who market slide and cassette labelers - Can you help? In-Reply-To: References: <1328041843.51913.YahooMailClassic@web162102.mail.bf1.yahoo.com> <97AA4AD3-2538-4D20-A7E5-E67B68EE67EB@email.arizona.edu> Message-ID: I am working on a project for NSH and CAP and I thought that the list might help me to avoid overlooking vendors that I may be unaware of. I am trying to identify ALL sources of cassette and slide labeling equipment. In order avoid clogging the list I invite you or vendor reps to email me directly. This may lead to an opportunity for companies to participate in this project. here is what I have thus far: Thermo Fisher General Data Healthcare Sakura Leica TBS (triangle biomedical) I know that Ventana markets a comprehensive software product that is used for specimen management but I don't know what that includes. I thought Dako may have had a competing product but I am unsure about this. I also know that in the past there were different versions of cassette printers from RA Lamb that were offered through different vendors, so for example the TBS and old Shandon/Thermo MicroWriters were different versions of the Lamb cassette printers. any information that you can offer will be greatly appreciated. thanks Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From kmerriam2003 <@t> yahoo.com Wed Feb 1 08:45:24 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Feb 1 08:45:33 2012 Subject: [Histonet] Immunofluorescence staining/minimizing background staining In-Reply-To: References: Message-ID: <1328107524.1076.YahooMailNeo@web130106.mail.mud.yahoo.com> Hi, ? What fluorochromes are you using?? There is a lot of autofluorescence in the FITC channel.? Have you looked at an unstained tissues under the scope with each filter that you need, that may give you a clue as to where your background is coming from.? In addition to an unstained slide, I suggest eliminating the primary to see if your secondary is sticking to the tissues. ? Are you doing single-color or double stains?? If you are getting a lot of autofluorescence with one fluorochrome, I would suggest trying a different one.? We use alexafluur-647 (far red) a lot because is it fairly clean (ie - very little autofluorescence in that channel) Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Kasai, Miki (NIH/NCI) [E]" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, January 30, 2012 4:50 PM Subject: [Histonet] Immunofluorescence staining/minimizing background staining Hi, We are performing some immunofluorescence staining on mouse lung tissue.? We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize the amount of background staining we are getting. We are titering our primary antibodies to find out optimal Ab concentration as well as the secondary conjugate Ab with the fluorophore of interest.? We use donkey serum for general blocking. Any other suggestions? Much appreciation, Miki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Feb 1 09:08:07 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Feb 1 09:08:12 2012 Subject: [Histonet] Bleaching in the histo lab In-Reply-To: <1328104647.83036.YahooMailClassic@web125402.mail.ne1.yahoo.com> References: <1328104647.83036.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: The short answer is that you need a detailed procedure for all immediate and regular cleaning of infectious materials and hazardous chemicals used in your lab. I believe the standard practice your safety officer is referring used in the clinical lab is a practice to clean all surfaces after each shift to remove/decontaminate all contaminated or potentially contaminated from the work surfaces of blood or other infectious material w/ 10% bleach solution (1:10 dilution of 5.25% solution of sodium hypochlorite) or other lab cleaners approved for biohazard approved contamination. The waste generated by this process should be disposed of in the non-regulated medical waste. Typically in the Histology room there should not be blood or other infectious materials (you are working w/ fixed and processed tissue samples), unless you have your frozen section and/or gross dissection processes connected to and part of the main Histology room. I suggest you use the bleach solution whenever there is known or suspicion of contamination of a potentially infectious material. For areas/surfaces and equipment where lab chemicals are used, always remove the spilled chemical according to MSDS recommendations and then clean the area w/ a damp cloth with water and a detergent and them wipe clean and dry. If or when a hazardous chemical is spilled (i.e. Xylene, Formalin or chemicals associated w/ IHC or Special staining), it should be treated as a hazardous chemical spill and there area should be cleaned according to your hazardous chemical spill protocol. Small spills (up to 300 cc) - neutralize and/or adsorption; medium spill (300 cc to 5 liters) - adsorption spill kit; Large spill (>5 liters) - outside help. disposal will be in regulated hazardous waste. The main point here, instances of contamination of infectious materials or any size spill of hazardous chemicals should be treated seriously and properly. Your cleaning and disposal procedure must be very detailed to protect the employees and meet lab, municipality, state and regulatory requirements. I am very passionate about properly handling chemicals and protecting everyone that must have contact w/ these necessary solutions/products. I suggest you and your safety officer have a sit down and discuss how to document and address these issues, a drive-by by a safety officer is really not adequate. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 1 Feb 2012 05:57:27 -0800 > From: we3smitty@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bleaching in the histo lab > > I have been told by our safety officer that it is standard practice too clean the lab at the end of the day with diluted bleach. I have noticed a chemical reaction (smell) when cleaning the main area of the lab. I have concerns that this is not a good practice due to chemical reactions as we use so many chemicals in histology. What do other people do? Also I believe it is unsafe to use bleach with anything formalin related. > Please let me know if you have a "standard" practice or mandated cleaning from your facility. > Angela > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carol.Freeman <@t> utoledo.edu Wed Feb 1 09:33:08 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Wed Feb 1 09:33:39 2012 Subject: [Histonet] IHCRG where are you? In-Reply-To: References: Message-ID: Good Morning, Just curious if any one out there knows what happened to the IHCRG (IHC review group?) It was an organization advertised in the NSH material I brought home from last September but the website doesn't exist and the email address listed was undeliverable...Just curious It sounded like an interest idea, just wondered what happened.. Thank you for any response :) From pathlocums <@t> gmail.com Wed Feb 1 11:33:43 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Feb 1 11:33:48 2012 Subject: [Histonet] RAC Medicare Audits - PAs Message-ID: <-1363935384787632281@unknownmsgid> Requirement by whom? Sent from my Windows Phone From: Richard Cartun Sent: 1/31/2012 4:20 PM To: Histonet Subject: [Histonet] RAC Medicare Audits - PAs Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From araniqkslvr <@t> yahoo.com Wed Feb 1 12:03:20 2012 From: araniqkslvr <@t> yahoo.com (Paula) Date: Wed Feb 1 12:03:27 2012 Subject: [Histonet] Re: Looking for Part-Time Volunteer Work Message-ID: <1328119400.2279.YahooMailClassic@web30302.mail.mud.yahoo.com> I am in Raleigh, NC. Looking for part-time volunteer work to build skills up. Paula From Fawn.Bomar <@t> HalifaxRegional.com Wed Feb 1 12:46:04 2012 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Feb 1 12:46:19 2012 Subject: [Histonet] CLIA Inspection Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B916B41A@EXCH-2K10.hrhs.com> Hi everyone! I was wondering if anyone out there that has knowledge of CLIA regs and inspections would be willing to contact me directly to help me answer a few questions. My contact info is Fawn.Bomar@halifaxregional.com or call if it is easier at 434-517-3033. Thank you in advance, Fawn From estellamireles <@t> gmail.com Wed Feb 1 13:24:48 2012 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Wed Feb 1 13:24:51 2012 Subject: [Histonet] Job Listing Houston,Tx Message-ID: ------------------------------ *ANNOUNCEMENT NUMBER: 14905- T* * * *JOB TITLE: Histology Technician II * * * *DEPARTMENT: Institute of Forensic Sciences* * * *HOURS: 7:30 a.m. ? 4:40 p.m. / Flexible* * Monday - Friday* * * *SALARY: Commensurate With Experience* * Based On 26 Pay Periods* * * *EDUCATION: *Completion of an Associate?s degree and completion of histology school with histo-technician certification (ASCP) American Society of Clinical Pathologist *at the time of employment or within one year of employment. * * * *EXPERIENCE: *One year of experience in a histology laboratory in which responsibilities included production of stained slides and preservation of tissue samples in both paraffin and formalin is *required*.** * * *JOB SKILLS: *The successful applicant must have expertise in the use of microtomes, manual staining procedures, manual slide coverslipping, automated slide stainers, automated slide coverslipper, tissue processors and tissue embedding. Must be capable of understanding and adhering to strict protocols for the handling, trimming and archival of tissue samples and blocks; knowledge of histology laboratory safety rules and procedures *is essential*. Good interpersonal skills *are a must*. *JOB DESCRIPTION: *Prepares stained slides of autopsy tissues in a careful, controlled environment. Prepares paraffin blocks of tissue for long-term storage and labels the samples in accordance with histology laboratory protocols; assists with archival of paraffin-embedded and non-paraffin-embedded formalin fixed tissues. Provides all tissue slides to the assigned Assistant Medical Examiner, Deputy Chief Medical Examiner or Chief Medical Examiner on a timely basis; assists with data entry into a computerized database to track laboratory efficiency as required. Other job assignments as assigned by the Deputy Chief Medical Examiner. *Position requires a high level of confidentiality, responsibility and dependability.* *PHYSICAL REQUIREMENTS:** *Must be able to sit and stand for prolonged periods of time; able to lift up to 40lbs; stooping and bending may be required. * * *EMPLOYMENT IS CONTINGENT UPON PASSING A CRIMINAL BACKGROUND CHECK.* * * * * *HARRIS COUNTY HAS AN EMPLOYMENT AT WILL POLICY.* *CLOSING DATE: Open Until Filled* *APPLY AT: 1310 PRAIRIE - SUITE 170* * * * * *UPON RECEIVING A CONDITIONAL OFFER OF EMPLOYMENT, ALL APPLICANTS ARE SCREENED FOR THE PRESENCE OF ILLEGAL DRUGS.*** From LRaff <@t> uropartners.com Wed Feb 1 13:37:55 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Feb 1 13:38:05 2012 Subject: [Histonet] On call Position in Chicago Message-ID: Our private outpatient specialty lab has an opening for an on-call part-time second shift and weekend histologist. We are located in the western Chicago suburbs about a mile east of Oak Brook Shopping Center. If interested, please contact Andrea O'Brien at 708-486-0076 . Experienced histologists only, please. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 From Rcartun <@t> harthosp.org Wed Feb 1 13:45:06 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Feb 1 13:45:24 2012 Subject: [Histonet] RAC Medicare Audits - PAs In-Reply-To: References: <4F283EDD.7400.0077.1@harthosp.org> Message-ID: <4F294FF2.7400.0077.1@harthosp.org> I don't know. I was hoping that there is someone in the Histonet community that is familiar with this. Richard >>> "McMahon, Loralee A" 2/1/2012 8:56 AM >>> I haven't heard that one. Do they mean present physically or present as in their office reading slides, but a phone call or page away? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Tuesday, January 31, 2012 7:19 PM To: Histonet Subject: [Histonet] RAC Medicare Audits - PAs Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Feb 1 14:17:14 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Feb 1 14:17:20 2012 Subject: [Histonet] C4d IF on FFPE kidney Message-ID: <138B99031E744674A4C020CB44691D27@dielangs.at> Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang From lpwenk <@t> sbcglobal.net Wed Feb 1 17:03:21 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Feb 1 17:03:28 2012 Subject: [Histonet] Bleaching in the histo lab In-Reply-To: <1328104647.83036.YahooMailClassic@web125402.mail.ne1.yahoo.com> References: <1328104647.83036.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: Several questions and comments, in no particular order: 1. What percent of bleach? - 10% is all that is needed for biohazards. If you are concerned about the smell, it might be too high a percent. 2. How good is your ventilation? How long do you continue to smell the chlorine? - If you continue to smell it hours later, or even the next day, have your safety officer and maintenance people check out the ventilation. 3. After wiping down with 10% bleach, are you wiping down the counter with water? - Need to clean off the corrosive bleach off the surfaces. That would also help with the smell. But takes more time. 3. What locations in the lab are you cleaning with dilute bleach? - The only areas that need to be cleaned with a disinfectant are those areas that have fresh or not completely fixed tissue, so around the grossing stations and the cryostat. Maybe where specimens are received into the lab. - The areas where you process tissue, embed, microtome, do staining, file slides and blocks should not need to be disinfected with bleach. The tissue has been fixed in formalin, and gone through alcohol, xylene (or substitute), and placed in 60 degree C (140 degree F) paraffin. That should kill almost all microorganisms. Therefore, should not need to clean up with anything beyond soap and water. If you have a very underprocessed tissue block, and it's oozing and weeping all over the counter and microtome, you may want to disinfect the area. (If it's a CJD case, you are going to need strong solutions than 10% bleach, but that's a whole new conversation.) - So talk with your safety officer, about how there are no biohazards in the other parts of the lab. They may be thinking more of the clinical pathology labs, with blood tubes and petri dishes, needing to be disinfected with bleach every day/shift. 4. Chemical incompatibility: Bleach is incompatible with ammonia (makes chlorine gas - deadly) Bleach is incompatible with acids Bleach is an oxidizer, and formaldehyde is supposed to be kept away from oxidizers. So, yes, I would be a little worried about chemical interaction. However, wiping down the area first with water, to remove other chemicals, before the bleach, would take care of this problems. 5. What does Epidemiology suggest for disinfectant? Our epidemiology is suggesting other cleaning solutions for disinfecting, rather than bleach, in many cases. - not as corrosive - less obnoxious fumes - more "green" - better disinfectant and faster, than bleach Peggy Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 (Comments reflect my opinions, not that of my hospital) -----Original Message----- From: angela smith Sent: Wednesday, February 01, 2012 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bleaching in the histo lab I have been told by our safety officer that it is standard practice too clean the lab at the end of the day with diluted bleach. I have noticed a chemical reaction (smell) when cleaning the main area of the lab. I have concerns that this is not a good practice due to chemical reactions as we use so many chemicals in histology. What do other people do? Also I believe it is unsafe to use bleach with anything formalin related. Please let me know if you have a "standard" practice or mandated cleaning from your facility. Angela _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Feb 1 18:26:45 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Feb 1 18:26:58 2012 Subject: [Histonet] RAC Medicare Audits - PAs In-Reply-To: <4F294FF2.7400.0077.1@harthosp.org> References: <4F283EDD.7400.0077.1@harthosp.org> <4F294FF2.7400.0077.1@harthosp.org> Message-ID: <65FAEDF0-7CFC-4188-8061-2382A42A7C00@yahoo.com> Sorry. But I thought RAC was a group of individuals who go over your billing looking for over payments to Medicare They typically get a % of what they find so it motivates them. If u have this group of auditors in your area and they Are saying this then I would just ask them to show you the rule they must be referring to a CLIA guideline somewhere. Best of luck Kim D Sent from my iPhone On Feb 1, 2012, at 2:45 PM, "Richard Cartun" wrote: > I don't know. I was hoping that there is someone in the Histonet community that is familiar with this. > > Richard > >>>> "McMahon, Loralee A" 2/1/2012 8:56 AM >>> > I haven't heard that one. Do they mean present physically or present as in their office reading slides, but a phone call or page away? > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] > Sent: Tuesday, January 31, 2012 7:19 PM > To: Histonet > Subject: [Histonet] RAC Medicare Audits - PAs > > Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Feb 1 18:51:45 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Feb 1 18:51:53 2012 Subject: [Histonet] Bleaching in the histo lab In-Reply-To: References: <1328104647.83036.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: Maybe those scented bleach wiped would work. I love those things and they smell pretty good. Just a thought. I like the greencan fresh scent products/clorox-disinfecting-wipes/ KimD Sent from my iPhone On Feb 1, 2012, at 6:03 PM, "Lee & Peggy Wenk" wrote: > > - Need to clean off the corrosive bleach off the surfaces. That would also From JMyers1 <@t> aol.com Thu Feb 2 05:38:33 2012 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Thu Feb 2 05:38:50 2012 Subject: [Histonet] IHCRG where are you? Message-ID: <9a4e.313de094.3c5bcfb9@aol.com> Carol: I'm happy to report that the IHC Resource Group remains alive and well. For reasons unkown, the group's website is currently not working properly, and through this message I've notified members of the NSH's staff of the problem. We are actively working on a number of projects, most notably the annual IHC Forum event, and once our site is working again we hope to post new information. Stay tuned for details... Sincerely, Joe Myers, M.S, CT(ASCP)QIHC Chair, IHC Resource Group ------------------------------ Message: 9 Date: Wed, 1 Feb 2012 10:33:08 -0500 From: "Freeman, Carol" Subject: [Histonet] IHCRG where are you? To: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Good Morning, Just curious if any one out there knows what happened to the IHCRG (IHC review group?) It was an organization advertised in the NSH material I brought home from last September but the website doesn't exist and the email address listed was undeliverable...Just curious It sounded like an interest idea, just wondered what happened.. Thank you for any response :) From gu.lang <@t> gmx.at Thu Feb 2 08:31:04 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Feb 2 08:31:15 2012 Subject: AW: [Histonet] C4d IF on FFPE kidney In-Reply-To: <4F295869.7400.0077.1@harthosp.org> References: <138B99031E744674A4C020CB44691D27@dielangs.at> <4F295869.7400.0077.1@harthosp.org> Message-ID: <206DA8F070EC4221B7C45F02B3A99C1C@dielangs.at> We have been performing C4d IHC on FFPE kidney for a couple of years. We receive only fixed samples. Yesterday my doctors came with this idea of IF - I still have to ask for the special reasons. But I have the suspicion, that they are not aware of the fact, that IF on frozen unfixed tissue is the usual way found in literature. There are some articles that deal with comparison of IF(frozen) and IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows additional staining in glomeruli. Perhaps someone told them, that IF is the golden standard and recommended. Gudrun -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Mittwoch, 01. Februar 2012 21:21 An: gu.lang@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Gudrun Lang" 2/1/2012 3:17 PM >>> Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kstoll <@t> mcw.edu Thu Feb 2 12:16:35 2012 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Thu Feb 2 12:16:42 2012 Subject: [Histonet] Topoisomerase I Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAD63@MCWMBX2.mcwcorp.net> I am using TOP1 that originally worked at 1:100 dilution. I am now using at 1:20. Any ideas why the staining has decreased so much? I have it stored in -20 in aliquots. Or has someone found a TOP1 that works for them. It is too expensive to run at 1:20. Thanks, Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu From MZHENG <@t> PARTNERS.ORG Thu Feb 2 12:51:10 2012 From: MZHENG <@t> PARTNERS.ORG (Zheng, Mei) Date: Thu Feb 2 12:51:26 2012 Subject: [Histonet] EGFR ISH Ventana Message-ID: <7C58021620332F438692F2B4EFF5782703C56A3A@PHSXMB26.partners.org> Hi Can someone help me with the EGFR ISH protocol for Ventana? Thanks! Mei Zheng, HTL(ASCP) QIHC Clinical Supervisor Immunohistochemistry Lab. Department of Pathology Brigham and Women's Hospital Boston, MA 02115 Lab Tel: 617 732-7790 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From kstoll <@t> mcw.edu Thu Feb 2 12:56:58 2012 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Thu Feb 2 12:57:07 2012 Subject: [Histonet] reagents without expiration dates Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? CAP checklist ANP 21382 Thanks, Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu From TGoins <@t> mt.gov Thu Feb 2 13:04:03 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Feb 2 13:04:19 2012 Subject: [Histonet] RE: reagents without expiration dates In-Reply-To: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> References: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> Message-ID: A general rule that I am familiar with is a maximum shelf-life of 1 year, basically due to repetitive access leading to eventual contamination. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stoll, Kathryn Sent: Thursday, February 02, 2012 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagents without expiration dates Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? CAP checklist ANP 21382 Thanks, Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Thu Feb 2 13:07:31 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Feb 2 13:07:39 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> References: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> Message-ID: Per SOP, we relabel, list date of receipt, test for quality and then apply a 12 month expiration date. We re-test after 12 months and continue to use, with 12 month dating, as long as the reagent meets quality standards set in the SOP. William DeSalvo, B.S., HTL(ASCP) > From: kstoll@mcw.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 2 Feb 2012 12:56:58 -0600 > Subject: [Histonet] reagents without expiration dates > > Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? > CAP checklist ANP 21382 > > Thanks, > Kathryn Stoll, HT(ASCP) > Depatment of Pathology > Medical College of Wisconsin > 9200 W Wisconsin Ave > Milwaukee WI 53226 > 414.805.1525 > kstoll@mcw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Feb 2 13:46:40 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Feb 2 13:46:46 2012 Subject: [Histonet] slides for frozens Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCD@PHSXMB30.partners.org> To all: We have been using the same slides for paraffins and frozens. We have no trouble with paraffin-embedded tissue adhering to the slides through the whole staining procedure. But our frozens (even 5um sections)have been falling off during manual staining. The slides are (+) charged. We used to use slides from Fisher, but they are so expensive, we changed to an independent vendor (half the price). Our procedure for frozen H&Es has recently changed: 1. Fix in 95% ETOH 1 min. 2. Hematoxylin 5 min. 3. Acetic acid H2O 2 quick dips 4. Wash-running H2O 10 seconds (10 dips) 5. Bluing reagent 10 dips 6. Wash-running H2O 10 seconds (or 10 dips) 7. Eosin 5 seconds 8. Dehydrate-95% ETOH 10 dips each 9. Dehydrate-100%ETOH 10 dips each 10.CitriSolv 10 dips each 11.Coverslip The tissue seems to fall off in the bluing reagent (we order from Mossberg). Can anyone help us? Suggest anything--what slides you are using? We have had the same problem with special stains (i.e. Oil Red O for fat on frozens). Thanks! Peggy P.S. I use the same slides for my 1um plastic embedding which I dip in water rinses and have no problem with the tissue falling off. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Thu Feb 2 13:56:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 2 13:56:55 2012 Subject: [Histonet] slides for frozens In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCD@PHSXMB30.partners.org> Message-ID: <1328212611.97248.YahooMailClassic@web162102.mail.bf1.yahoo.com> There is a known saying that goes: "You get what you pay for". Ren? J. --- On Thu, 2/2/12, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] slides for frozens To: "histonet" Date: Thursday, February 2, 2012, 2:46 PM To all: We have been using the same slides for paraffins and frozens.? We have no trouble with paraffin-embedded tissue adhering to the slides through the whole staining procedure.? But our frozens (even 5um sections)have been falling off during manual staining.? The slides are (+) charged.? We used to use slides from Fisher, but they are so expensive, we changed to an independent vendor (half the price). Our procedure for frozen H&Es has recently changed: 1. Fix in 95% ETOH? ? ???1 min. 2. Hematoxylin? ? ? ? ???5 min. 3. Acetic acid H2O?????2 quick dips 4. Wash-running H2O? ? ? 10 seconds (10 dips) 5. Bluing reagent??? ? ? ???10 dips 6. Wash-running H2O? ? ? 10 seconds (or 10 dips) 7. Eosin??? ? ? ? ? ? ???5 seconds 8. Dehydrate-95% ETOH? ? 10 dips each 9. Dehydrate-100%ETOH? ? 10 dips each 10.CitriSolv? ? ? ? ? ???10 dips each 11.Coverslip The tissue seems to fall off in the bluing reagent (we order from Mossberg). Can anyone help us?? Suggest anything--what slides you are using?? We have had the same problem with special stains (i.e. Oil Red O for fat on frozens). Thanks! Peggy P.S.? I use the same slides for my 1um plastic embedding which I dip in water rinses and have no problem with the tissue falling off. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Feb 2 14:03:35 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Feb 2 14:03:39 2012 Subject: [Histonet] slides for frozens In-Reply-To: <1328212611.97248.YahooMailClassic@web162102.mail.bf1.yahoo.com> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCD@PHSXMB30.partners.org> <1328212611.97248.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCE@PHSXMB30.partners.org> That may be true, but I don't recall having this problem with our previous protocol (which was longer and included a fix in alcoholic formalin). I think we will try the "old" method and see what happens. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, February 02, 2012 2:57 PM To: histonet; Sherwood, Margaret Subject: Re: [Histonet] slides for frozens There is a known saying that goes: "You get what you pay for". Ren? J. --- On Thu, 2/2/12, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] slides for frozens To: "histonet" Date: Thursday, February 2, 2012, 2:46 PM To all: We have been using the same slides for paraffins and frozens. We have no trouble with paraffin-embedded tissue adhering to the slides through the whole staining procedure. But our frozens (even 5um sections)have been falling off during manual staining. The slides are (+) charged. We used to use slides from Fisher, but they are so expensive, we changed to an independent vendor (half the price). Our procedure for frozen H&Es has recently changed: 1. Fix in 95% ETOH 1 min. 2. Hematoxylin 5 min. 3. Acetic acid H2O 2 quick dips 4. Wash-running H2O 10 seconds (10 dips) 5. Bluing reagent 10 dips 6. Wash-running H2O 10 seconds (or 10 dips) 7. Eosin 5 seconds 8. Dehydrate-95% ETOH 10 dips each 9. Dehydrate-100%ETOH 10 dips each 10.CitriSolv 10 dips each 11.Coverslip The tissue seems to fall off in the bluing reagent (we order from Mossberg). Can anyone help us? Suggest anything--what slides you are using? We have had the same problem with special stains (i.e. Oil Red O for fat on frozens). Thanks! Peggy P.S. I use the same slides for my 1um plastic embedding which I dip in water rinses and have no problem with the tissue falling off. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 2 14:08:02 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 2 14:08:08 2012 Subject: [Histonet] slides for frozens In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCE@PHSXMB30.partners.org> Message-ID: <1328213282.15651.YahooMailClassic@web162102.mail.bf1.yahoo.com> That could be the solution. The problem is that you changed 2 things: the protocol and the slides and now you do not know which is "the culprit". Returning to the old protocols is a first step to trying to solve the present situation and, as you can imagine, if the old protocol does not solve the problem, the slides may be the cause. Ren? J. --- On Thu, 2/2/12, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] slides for frozens To: "Rene J Buesa" , "histonet" Date: Thursday, February 2, 2012, 3:03 PM That may be true, but I don't recall having this problem with our previous protocol (which was longer and included a fix in alcoholic formalin).? I think we will try the "old" method and see what happens. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, February 02, 2012 2:57 PM To: histonet; Sherwood, Margaret Subject: Re: [Histonet] slides for frozens There is a known saying that goes: "You get what you pay for". Ren? J. --- On Thu, 2/2/12, Sherwood, Margaret wrote: ??? From: Sherwood, Margaret ??? Subject: Re: [Histonet] slides for frozens ??? To: "histonet" ??? Date: Thursday, February 2, 2012, 2:46 PM ??? ??? ??? To all: ??? ??? We have been using the same slides for paraffins and frozens.? We have no ??? trouble with paraffin-embedded tissue adhering to the slides through the whole ??? staining procedure.? But our frozens (even 5um sections)have been falling off ??? during manual staining.? The slides are (+) charged.? We used to use slides from ??? Fisher, but they are so expensive, we changed to an independent vendor (half the ??? price). ??? ??? Our procedure for frozen H&Es has recently changed: ??? 1. Fix in 95% ETOH? ? ???1 min. ??? 2. Hematoxylin? ? ? ? ???5 min. ??? 3. Acetic acid H2O? ???2 quick dips ??? 4. Wash-running H2O? ? ? 10 seconds (10 dips) ??? 5. Bluing reagent? ? ? ? ???10 dips ??? 6. Wash-running H2O? ? ? 10 seconds (or 10 dips) ??? 7. Eosin? ? ? ? ? ? ? ???5 seconds ??? 8. Dehydrate-95% ETOH? ? 10 dips each ??? 9. Dehydrate-100%ETOH? ? 10 dips each ??? 10.CitriSolv? ? ? ? ? ???10 dips each ??? 11.Coverslip ??? ??? The tissue seems to fall off in the bluing reagent (we order from Mossberg). ??? ??? Can anyone help us?? Suggest anything--what slides you are using?? We have had ??? the same problem with special stains (i.e. Oil Red O for fat on frozens). ??? ??? Thanks! ??? Peggy ??? ??? P.S.? I use the same slides for my 1um plastic embedding which I dip in water ??? rinses and have no problem with the tissue falling off. ??? ??? ??? Peggy Sherwood ??? Lab Associate, Photopathology ??? Wellman Center for Photomedicine (EDR 214) ??? Massachusetts General Hospital ??? 50 Blossom Street ??? Boston, MA 02114-2696 ??? 617-724-4839 (voice mail) ??? 617-726-6983 (lab) ??? 617-726-1206 (fax) ??? msherwood@partners.org ??? ??? ??? ??? The information in this e-mail is intended only for the person to whom it is ??? addressed. If you believe this e-mail was sent to you in error and the e-mail ??? contains patient information, please contact the Partners Compliance HelpLine at ??? http://www.partners.org/complianceline . If the e-mail was sent to you in error ??? but does not contain patient information, please contact the sender and properly ??? dispose of the e-mail. ??? ??? ??? _______________________________________________ ??? Histonet mailing list ??? Histonet@lists.utsouthwestern.edu ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? From talulahgosh <@t> gmail.com Thu Feb 2 14:33:10 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Feb 2 14:33:17 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: References: <110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> Message-ID: I've always wanted to have a contest to see who had the oldest reagents. My lab once had something that was 20 years old. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > Per SOP, we relabel, list date of receipt, test for quality and then apply > a 12 month expiration date. We re-test after 12 months and continue to use, > with 12 month dating, as long as the reagent meets quality standards set in > the SOP. > > William DeSalvo, B.S., HTL(ASCP) > > > > > From: kstoll@mcw.edu > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > Subject: [Histonet] reagents without expiration dates > > > > Could anyone share a policy to deal with regents that do not have a > manufacturer's expiration date? > > CAP checklist ANP 21382 > > > > Thanks, > > Kathryn Stoll, HT(ASCP) > > Depatment of Pathology > > Medical College of Wisconsin > > 9200 W Wisconsin Ave > > Milwaukee WI 53226 > > 414.805.1525 > > kstoll@mcw.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Thu Feb 2 15:11:12 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Feb 2 15:11:17 2012 Subject: [Histonet] histotech needed Message-ID: <25A4DE08332B19499904459F00AAACB719ACB9CBF2@EVS1.archildrens.org> Arkansas Children's Hospital is looking for a registered histotech. This is a dayshift, Monday-Friday position with rotating holidays on call. Our lab performs routine H&E's, special stains by hand and we have a Bond III immunostainer. We process approximately 6000 surgicals a year. ACH has excellent benefits. Little Rock is nice small city in which to live. We have many area attractions and if you like the outdoor life you will love Arkansas. The position is posted as an MLT, histology lab. Apply online at: www.archildrens.org Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Feb 2 15:14:53 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 2 15:14:58 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: Message-ID: <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> Ha.Ha,Ha!!!!!!!!!!! I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). Try to beat that!. Ren? J. --- On Thu, 2/2/12, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] reagents without expiration dates To: histonet@lists.utsouthwestern.edu Date: Thursday, February 2, 2012, 3:33 PM I've always wanted to have a contest to see who had the oldest reagents. My lab once had something that was 20 years old. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > Per SOP, we relabel, list date of receipt, test for quality and then apply > a 12 month expiration date. We re-test after 12 months and continue to use, > with 12 month dating, as long as the reagent meets quality standards set in > the SOP. > > William DeSalvo, B.S., HTL(ASCP) > > > > > From: kstoll@mcw.edu > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > Subject: [Histonet] reagents without expiration dates > > > > Could anyone share a policy to deal with regents that do not have a > manufacturer's expiration date? > > CAP checklist ANP 21382 > > > > Thanks, > > Kathryn Stoll, HT(ASCP) > > Depatment of Pathology > > Medical College of Wisconsin > > 9200 W Wisconsin Ave > > Milwaukee WI 53226 > > 414.805.1525 > > kstoll@mcw.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Feb 2 16:41:07 2012 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Feb 2 16:41:17 2012 Subject: [Histonet] RE: Interviewing Histotechs... In-Reply-To: <782B4970-9895-4DFD-8AE9-563A1842E26A@yahoo.com> References: <782B4970-9895-4DFD-8AE9-563A1842E26A@yahoo.com> Message-ID: <8E6FB415E5AB409C90E5494BC877D04F@JoePC> yeah, I never did like that the "a monkey can do it" crap. A pathologist told me that he could teach a monkey to gross. When the grossing tech messed up a case, I was called in and got jumped on. He really didn't appreciate it when I told him he better get that monkey trained quick. JTT ----- Original Message ----- From: "Kim Donadio" To: "Jerry Ricks" Cc: Sent: Tuesday, January 31, 2012 12:46 PM Subject: Re: [Histonet] RE: Interviewing Histotechs... Your comment about a monkey hits a nerve. There is a misconception I think in our field that yes any monkey off the street can do our job. Well they can't. It takes a good amount of knowledge to understand tissues, stains, chemical reactions and yes you will need to have some amount of hand eye coordination Skill . It's the monkey theory that has histotechs jumping up and down into these quick almost no hands on programs so they can get a good paying job with not much invested I'm afraid. Yes, I'm an expert at sticking my foot in my mouth. But if we as a group don't recognize why we are even having this debate about testing techs on the most basic of functions. Then I worry about the future of our profession And even healthcare because by god if you want monkeys , think monkeys , you will get monkeys! And darn it. I can't run and hide from this one can I lol Have a great week! Kim D Sent from my iPhone On Jan 31, 2012, at 1:13 PM, Jerry Ricks wrote: > > > > > Hi Toysha > > I think I'm just coming at it from "research mode" not clinical. Hands on > Histotechnology is a core part of our work, but just part, and it is > focused on animal models of cardiovascular disease. Depending on whether > the researcher is a postdoc or an undergrad they will have more or fewer > general lab skills including histo skills. I haven't met anyone yet who > did not need some training for embedding of brachiocephalic arteries of > mice. > > I doubt I would do well in a clinical lab. I've become accustomed to docs > saying "wow that's beautiful can you teach me how to do that?" I gather > in the clinical field it's more like "a monkey can learn how to section." > Maybe a good monkey could but I doubt it could work up an IHC with a new > antibody. > > > Jerry > > >> From: TNMayer@mdanderson.org >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 31 Jan 2012 10:38:58 -0600 >> Subject: [Histonet] RE: Interviewing Histotechs... >> >> Jerry, >> I agree with you somewhat. I have met techs that misrepresented >> themselves and said that they could cut or embed, and knew how to operate >> the instruments, but could not produce quality work. You are right when >> you said that it is different for clinical vs. research. I have almost >> always worked clinical, and noticed that when working with research >> techs, they had a difficult time adjusting to clinical with the time >> frames and quality. >> When training new hires, depending on the position I am hiring for, I >> expect to train in the new workflow that they have learned, the new >> instrument they use, not the basic skills. I only expect to do that with >> a student. Fresh techs are expected to know how to get a section, not cut >> the plastic on the block, embed skin, and set up the h&e stainer. I >> should only have to go over and orient them on "our procedure" not teach >> the skill. >> I have worked various part-time jobs over the years and the first thing I >> ask is 'how many microns do you cut at here'? While 3-4 is the standard, >> some labs want everything at 3, or some at 4. I know how to cut, but >> like you it takes about 2 weeks to get used to the new instrument. That's >> fine, but I don't expect to have to teach the tech how to embed a skin or >> cut a kidney biopsy. Not for an experienced tech, unless they have never >> encountered it. That has to be made known during the interview. >> Yes, a cutting test is good, I have seen registered techs not make it >> past probation (90 days) because they could not cut. It would have saved >> the company time and MONEY if a test could have been given. Asking if >> they can cut a kidney biopsy, or embed a skin would be good as well. I >> can't go back and get more epithelia or ask for another pass through the >> kidney. >> >> >> >> Toysha N. Mayer, MBA, HT (ASCP) >> Instructor, Education Coordinator >> Program in Histotechnology >> School of Health Professions >> MD Anderson Cancer Center >> (713) 563-3481 >> tnmayer@mdanderson.org >> >> >> >> >> >> >> Message: 5 >> Date: Mon, 30 Jan 2012 11:13:11 -0800 >> From: Jerry Ricks >> Subject: [Histonet] Interviewing Histotechs... >> To: >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> >> I gather it is different in clinical labs than in research labs. In >> clinical labs there is an emphasis on quantity and speed. In research >> the emphasis is on doing good experiments. Our "patients" are almost >> always deceased or shortly about to be so there is no urgency of >> diagnosis factor. For us, "diagnosis" means making precise measurements >> else some scientists looking at an image and asking each other "what >> the?" >> >> Anyway I always assume that the person I am hiring is incompetent at >> histology and that they will need to be personally trained by me. >> Doesn't matter how much experience they have. And over 23 years that has >> turned out to be true. I've met exactly two people who didn't need much >> training. One was a former senior clinical lab manager. The other was >> a kid straight out of high school who happened to have a histology >> experience from high school and a decent histo portfolio. Yes, Mercer >> Island High School had a Histology program. >> >> No such thing as a tech who doesn't need to be trained and any tech >> trained by me will be up and running in a week or two. Why bother making >> them cut or stain anything during a darn interview. If they are smart >> and cooperative they will work out. >> >> If I ever go to a new lab with a new microtome, new protocols, I am >> pretty sure that I will be sort of incompetent for a week or two as well. >> >> Jerry Ricks >> Research Scientist >> University of Washington >> Department of Pathology >> >> >> >> histonet@lists.utsouthwestern.edu >>> Date: Sun, 29 Jan 2012 13:12:09 -0500 >>> From: rsrichmond@gmail.com >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Re: interview.... >>> >>> Ray Koelling asked me: >>> >>>>> If the Samurai Pathologist is out there reading still; any idea over >>>>> your career, about how many glass slides have you viewed under a >>>>> microscope since the first? Your replies are always top-notch, >>>>> entertaining and informative. And hope with each new job you don't >>>>> have to show someone you can pass a test of which slide shows normal >>>>> liver and which slide shows cirrhotic liver in your interview.<< >>> >>> I really have no idea how many slides. In a normal year I sign out >>> about 3,000 histology cases (remember I don't work full time) >>> averaging maybe 3 slides per case. >>> >>> Generally I've gotten jobs, both private clients and agency clients, >>> by recommendation. A number of years ago I was interviewed by a >>> four-pathologist hospital group who handed me a tray of 20 slides with >>> the necessary historical information, and was told that this was a set >>> the group had collected, including very straightforward cases, cases >>> with serious diagnostic pitfalls, and some cases they'd never been >>> able to make a diagnosis on. They tried to make it a test of judgment >>> rather than simple diagnostic skill. Told to take as much time as I >>> needed. I guess I passed - by coincidence, the entire group chanced to >>> break up very quickly, and an entirely different team took over. >>> >>> Bob Richmond >>> Samurai Pathologist >>> Knoxville TN >>> >> ************************************ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Feb 2 16:50:10 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Feb 2 16:50:19 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: OK, I'll give it a try Ren?. "Baker's Analyzed" Potassium Permanganate (5 lbs) with a "typed" label made with an actual "typewriter". Trying to verify age but I've wasted enough time already on fun stuff today. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 02, 2012 2:15 PM To: histonet@lists.utsouthwestern.edu; Emily Sours Subject: Re: [Histonet] reagents without expiration dates Ha.Ha,Ha!!!!!!!!!!! I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). Try to beat that!. Ren? J. --- On Thu, 2/2/12, Emily Sours > wrote: From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates To: histonet@lists.utsouthwestern.edu Date: Thursday, February 2, 2012, 3:33 PM I've always wanted to have a contest to see who had the oldest reagents. My lab once had something that was 20 years old. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO >wrote: > > Per SOP, we relabel, list date of receipt, test for quality and then > apply a 12 month expiration date. We re-test after 12 months and > continue to use, with 12 month dating, as long as the reagent meets > quality standards set in the SOP. > > William DeSalvo, B.S., HTL(ASCP) > > > > > From: kstoll@mcw.edu > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > Subject: [Histonet] reagents without expiration dates > > > > Could anyone share a policy to deal with regents that do not have a > manufacturer's expiration date? > > CAP checklist ANP 21382 > > > > Thanks, > > Kathryn Stoll, HT(ASCP) > > Depatment of Pathology > > Medical College of Wisconsin > > 9200 W Wisconsin Ave > > Milwaukee WI 53226 > > 414.805.1525 > > kstoll@mcw.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Thu Feb 2 17:02:34 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Feb 2 17:03:28 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: , <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 2 Feb 2012 13:14:53 -0800 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com > Subject: Re: [Histonet] reagents without expiration dates > CC: > > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > > > > Per SOP, we relabel, list date of receipt, test for quality and then apply > > a 12 month expiration date. We re-test after 12 months and continue to use, > > with 12 month dating, as long as the reagent meets quality standards set in > > the SOP. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > From: kstoll@mcw.edu > > > To: histonet@lists.utsouthwestern.edu > > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > > Subject: [Histonet] reagents without expiration dates > > > > > > Could anyone share a policy to deal with regents that do not have a > > manufacturer's expiration date? > > > CAP checklist ANP 21382 > > > > > > Thanks, > > > Kathryn Stoll, HT(ASCP) > > > Depatment of Pathology > > > Medical College of Wisconsin > > > 9200 W Wisconsin Ave > > > Milwaukee WI 53226 > > > 414.805.1525 > > > kstoll@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Feb 2 17:07:20 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Feb 2 17:07:27 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: Message-ID: I had a student that was working in a lab with old dry powders. One that I recall had a "date opened" of 1942. WILLIAM DESALVO Sent by: histonet-bounces@lists.utsouthwestern.edu 02/02/2012 03:04 PM To , histonet , cc Subject RE: [Histonet] reagents without expiration dates Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 2 Feb 2012 13:14:53 -0800 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com > Subject: Re: [Histonet] reagents without expiration dates > CC: > > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > > > > Per SOP, we relabel, list date of receipt, test for quality and then apply > > a 12 month expiration date. We re-test after 12 months and continue to use, > > with 12 month dating, as long as the reagent meets quality standards set in > > the SOP. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > From: kstoll@mcw.edu > > > To: histonet@lists.utsouthwestern.edu > > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > > Subject: [Histonet] reagents without expiration dates > > > > > > Could anyone share a policy to deal with regents that do not have a > > manufacturer's expiration date? > > > CAP checklist ANP 21382 > > > > > > Thanks, > > > Kathryn Stoll, HT(ASCP) > > > Depatment of Pathology > > > Medical College of Wisconsin > > > 9200 W Wisconsin Ave > > > Milwaukee WI 53226 > > > 414.805.1525 > > > kstoll@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Thu Feb 2 17:14:24 2012 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Thu Feb 2 17:14:55 2012 Subject: AW: [Histonet] C4d IF on FFPE kidney In-Reply-To: <206DA8F070EC4221B7C45F02B3A99C1C@dielangs.at> References: <138B99031E744674A4C020CB44691D27@dielangs.at> <4F295869.7400.0077.1@harthosp.org> <206DA8F070EC4221B7C45F02B3A99C1C@dielangs.at> Message-ID: <4F2BA56F.411C.0039.0@health.qld.gov.au> Hi Gudren We have been doing both IF and IHC for C4d for quite a while. While we have found that the IF staining is superior to the IHC we are not unhappy with the IHC results. Our IHC results have improved since getting a Ventana Ultra which has allowed better control of the staining. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Gudrun Lang" 2/3/2012 12:31 am >>> We have been performing C4d IHC on FFPE kidney for a couple of years. We receive only fixed samples. Yesterday my doctors came with this idea of IF - I still have to ask for the special reasons. But I have the suspicion, that they are not aware of the fact, that IF on frozen unfixed tissue is the usual way found in literature. There are some articles that deal with comparison of IF(frozen) and IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows additional staining in glomeruli. Perhaps someone told them, that IF is the golden standard and recommended. Gudrun -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Mittwoch, 01. Februar 2012 21:21 An: gu.lang@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Gudrun Lang" 2/1/2012 3:17 PM >>> Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From heather.mcleod <@t> webmail.co.za Fri Feb 3 02:48:25 2012 From: heather.mcleod <@t> webmail.co.za (Heather Mcleod) Date: Fri Feb 3 02:48:36 2012 Subject: [Histonet] hrp vs alk phos In-Reply-To: <206DA8F070EC4221B7C45F02B3A99C1C@dielangs.at> References: <138B99031E744674A4C020CB44691D27@dielangs.at> <4F295869.7400.0077.1@harthosp.org>, <206DA8F070EC4221B7C45F02B3A99C1C@dielangs.at> Message-ID: Dear Histonetters, Our pathologist wants us to change from HRP to ALK PHOS. We run a Ventana XT using the titration option. Fifteen of our 20 antibody selection are giving the same result when run concurrently with the 2 detection systems. The other 5 stain perfectly with the HRP kit and gives NO staining with the ALK PHOS kit. We use the same protocol except for the kit selection and the titrated antibody is taken from the same vial for the respective pairs. Has anybody had a problem like this? The answer may be obvious but we cannot see it ..... yet. Please help Many many thanks Heather On Thu, 2 Feb 2012 15:31:04 +0100 "Gudrun Lang" wrote > We have been performing C4d IHC on FFPE kidney for a couple of years. We > receive only fixed samples. > Yesterday my doctors came with this idea of IF - I still have to ask for the > special reasons. But I have the suspicion, that they are not aware of the > fact, that IF on frozen unfixed tissue is the usual way found in literature. > > There are some articles that deal with comparison of IF(frozen) and > IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows > additional staining in glomeruli. > > Perhaps someone told them, that IF is the golden standard and recommended. > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: Richard Cartun [mailto:Rcartun@harthosp.org] > Gesendet: Mittwoch, 01. Februar 2012 21:21 > An: gu.lang@gmx.at > Betreff: Re: [Histonet] C4d IF on FFPE kidney > > Hi Gudrun: > > Is there reason why you want to use IF and not immunoperoxidase? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Gudrun Lang" 2/1/2012 3:17 PM >>> > Hi! > > Can someone provide me a immunofluorescence protocol for C4d on formalin > fixed human paraffin sections? > > > > thanks in advance > > > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________ South Africas premier free email service - www.webmail.co.za For super low premiums, click here. http://www.dialdirect.co.za/?vdn=15828 From mturner <@t> CSILaboratories.com Fri Feb 3 07:32:44 2012 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Feb 3 07:32:51 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: References: , <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: <467983BDAC5880438E2202F6B9BF79A832FCF3@exchange-be-01.CSI-LABS.local> I once worked for a lab in the Midwest in the early 80's and was surprised to find a quart-sized bottle of very dehydrated powder labeled "Picric Acid" with a date of 1962 on it. Yellow granular dust covered the top. I immediately notified our security folks, who called in the local bomb squad. The squad took the bottle out into the field next to the hospital and detonated it. Left a nice little crater... You can say I started my career with a "Bang!" :-) Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Thursday, February 02, 2012 6:03 PM To: rjbuesa@yahoo.com; histonet; talulahgosh@gmail.com Subject: RE: [Histonet] reagents without expiration dates Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 2 Feb 2012 13:14:53 -0800 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com > Subject: Re: [Histonet] reagents without expiration dates > CC: > > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon > up to 600 pounds and die of a heart attack at 43, you can! You are > free to do so. To me, that's beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > > > > Per SOP, we relabel, list date of receipt, test for quality and then > > apply a 12 month expiration date. We re-test after 12 months and > > continue to use, with 12 month dating, as long as the reagent meets > > quality standards set in the SOP. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > From: kstoll@mcw.edu > > > To: histonet@lists.utsouthwestern.edu > > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > > Subject: [Histonet] reagents without expiration dates > > > > > > Could anyone share a policy to deal with regents that do not have > > > a > > manufacturer's expiration date? > > > CAP checklist ANP 21382 > > > > > > Thanks, > > > Kathryn Stoll, HT(ASCP) > > > Depatment of Pathology > > > Medical College of Wisconsin > > > 9200 W Wisconsin Ave > > > Milwaukee WI 53226 > > > 414.805.1525 > > > kstoll@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Feb 3 08:30:56 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri Feb 3 08:31:04 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: <467983BDAC5880438E2202F6B9BF79A832FCF3@exchange-be-01.CSI-LABS.local> References: , <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> <467983BDAC5880438E2202F6B9BF79A832FCF3@exchange-be-01.CSI-LABS.local> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DD5@PHSXMB30.partners.org> You're lucky it didn't blow up the lab! Our safety people do a yearly inspection of chemicals etc. and once fined and closed down a lab for keeping dried up picric acid. Not pretty! Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Friday, February 03, 2012 8:33 AM To: histonet Subject: RE: [Histonet] reagents without expiration dates I once worked for a lab in the Midwest in the early 80's and was surprised to find a quart-sized bottle of very dehydrated powder labeled "Picric Acid" with a date of 1962 on it. Yellow granular dust covered the top. I immediately notified our security folks, who called in the local bomb squad. The squad took the bottle out into the field next to the hospital and detonated it. Left a nice little crater... You can say I started my career with a "Bang!" :-) Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Thursday, February 02, 2012 6:03 PM To: rjbuesa@yahoo.com; histonet; talulahgosh@gmail.com Subject: RE: [Histonet] reagents without expiration dates Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 2 Feb 2012 13:14:53 -0800 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com > Subject: Re: [Histonet] reagents without expiration dates > CC: > > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon > up to 600 pounds and die of a heart attack at 43, you can! You are > free to do so. To me, that's beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > > > > Per SOP, we relabel, list date of receipt, test for quality and then > > apply a 12 month expiration date. We re-test after 12 months and > > continue to use, with 12 month dating, as long as the reagent meets > > quality standards set in the SOP. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > From: kstoll@mcw.edu > > > To: histonet@lists.utsouthwestern.edu > > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > > Subject: [Histonet] reagents without expiration dates > > > > > > Could anyone share a policy to deal with regents that do not have > > > a > > manufacturer's expiration date? > > > CAP checklist ANP 21382 > > > > > > Thanks, > > > Kathryn Stoll, HT(ASCP) > > > Depatment of Pathology > > > Medical College of Wisconsin > > > 9200 W Wisconsin Ave > > > Milwaukee WI 53226 > > > 414.805.1525 > > > kstoll@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mcauliff <@t> umdnj.edu Fri Feb 3 08:57:34 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 3 08:56:33 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: <4F2BF5DE.8040808@umdnj.edu> I have some Grubler dyes in the original containers. Probably pre-WWII but not dated. Geoff On 2/2/2012 4:14 PM, Rene J Buesa wrote: > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVOwrote: > >> Per SOP, we relabel, list date of receipt, test for quality and then apply >> a 12 month expiration date. We re-test after 12 months and continue to use, >> with 12 month dating, as long as the reagent meets quality standards set in >> the SOP. >> >> William DeSalvo, B.S., HTL(ASCP) >> >> >> >>> From: kstoll@mcw.edu >>> To: histonet@lists.utsouthwestern.edu >>> Date: Thu, 2 Feb 2012 12:56:58 -0600 >>> Subject: [Histonet] reagents without expiration dates >>> >>> Could anyone share a policy to deal with regents that do not have a >> manufacturer's expiration date? >>> CAP checklist ANP 21382 >>> >>> Thanks, >>> Kathryn Stoll, HT(ASCP) >>> Depatment of Pathology >>> Medical College of Wisconsin >>> 9200 W Wisconsin Ave >>> Milwaukee WI 53226 >>> 414.805.1525 >>> kstoll@mcw.edu >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mturner <@t> CSILaboratories.com Fri Feb 3 09:06:44 2012 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Feb 3 09:06:54 2012 Subject: [Histonet] reagents without expiration dates In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DD5@PHSXMB30.partners.org> References: , <1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> <467983BDAC5880438E2202F6B9BF79A832FCF3@exchange-be-01.CSI-LABS.local> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DD5@PHSXMB30.partners.org> Message-ID: <467983BDAC5880438E2202F6B9BF79A832FD47@exchange-be-01.CSI-LABS.local> The Security Director turned a very pale shade of yellow when he witnessed the strength of the explosion. Just glad I caught it before someone tried to unscrew the lid... Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Friday, February 03, 2012 9:31 AM To: Mark Turner; histonet Subject: RE: [Histonet] reagents without expiration dates You're lucky it didn't blow up the lab! Our safety people do a yearly inspection of chemicals etc. and once fined and closed down a lab for keeping dried up picric acid. Not pretty! Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Friday, February 03, 2012 8:33 AM To: histonet Subject: RE: [Histonet] reagents without expiration dates I once worked for a lab in the Midwest in the early 80's and was surprised to find a quart-sized bottle of very dehydrated powder labeled "Picric Acid" with a date of 1962 on it. Yellow granular dust covered the top. I immediately notified our security folks, who called in the local bomb squad. The squad took the bottle out into the field next to the hospital and detonated it. Left a nice little crater... You can say I started my career with a "Bang!" :-) Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Thursday, February 02, 2012 6:03 PM To: rjbuesa@yahoo.com; histonet; talulahgosh@gmail.com Subject: RE: [Histonet] reagents without expiration dates Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use. William DeSalvo, B.S., HTL(ASCP) > Date: Thu, 2 Feb 2012 13:14:53 -0800 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com > Subject: Re: [Histonet] reagents without expiration dates > CC: > > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon > up to 600 pounds and die of a heart attack at 43, you can! You are > free to do so. To me, that's beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > > > > > Per SOP, we relabel, list date of receipt, test for quality and then > > apply a 12 month expiration date. We re-test after 12 months and > > continue to use, with 12 month dating, as long as the reagent meets > > quality standards set in the SOP. > > > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > > > From: kstoll@mcw.edu > > > To: histonet@lists.utsouthwestern.edu > > > Date: Thu, 2 Feb 2012 12:56:58 -0600 > > > Subject: [Histonet] reagents without expiration dates > > > > > > Could anyone share a policy to deal with regents that do not have > > > a > > manufacturer's expiration date? > > > CAP checklist ANP 21382 > > > > > > Thanks, > > > Kathryn Stoll, HT(ASCP) > > > Depatment of Pathology > > > Medical College of Wisconsin > > > 9200 W Wisconsin Ave > > > Milwaukee WI 53226 > > > 414.805.1525 > > > kstoll@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From GaleL <@t> unionhospital.org Fri Feb 3 09:24:14 2012 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Fri Feb 3 09:24:23 2012 Subject: [Histonet] Breast IHC testing Message-ID: I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From trathborne <@t> somerset-healthcare.com Fri Feb 3 09:28:33 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Feb 3 09:28:37 2012 Subject: [Histonet] RE: Breast IHC testing In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F8083D@SMCMAIL01.somerset-healthcare.com> When these guidelines were originally published, we moved some per diem hours from a Monday to a Sunday. This has assured minimum as well as maximum fixation times. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Melissa.Kuhnla <@t> chsli.org Fri Feb 3 09:48:22 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Feb 3 09:49:14 2012 Subject: [Histonet] xylene substitute and IHC Message-ID: Hello, Is anyone out there using a Xylene substitute during tissue processing? When you switched what form validation did you perform on your IHC stains? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From JWeems <@t> sjha.org Fri Feb 3 09:58:05 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Feb 3 09:58:10 2012 Subject: [Histonet] RE: Breast IHC testing In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846E42277@CHEXCMS10.one.ads.che.org> We have a med tech take the tissue off the processor for us. It waits very patiently till we get in on Monday to embed it! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 10:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From victoria.spoon <@t> bassett.org Fri Feb 3 10:02:21 2012 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Fri Feb 3 10:02:25 2012 Subject: [Histonet] Position in Cooperstown NY In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640846E42277@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640846E42277@CHEXCMS10.one.ads.che.org> Message-ID: <3D002D8531A0C743A35B817037E5AECA0420C6@EX08.bassett.org> Bassett Hospital in Cooperstown NY has an opening for a histotechnician. This is a full time day position M-F 7:30 to 4 pm. Responsibilities include a full range of laboratories duties for a histotechnician including embedding, microtomy and routine, special and immunoperoxidase staining. Bassett Hospital supports the Network's 5 institutions' pathology needs, providing a large variety of tissue types in a mid level volume setting. The histotechnicians rotate weekly through of the all of disciplines maintaining competency in all areas. We have a wide range of industry leading equipment while being able to maintain hands on technical skills and close contact with 5 pathologists and attending providers. Candidates must be qualified as a Clinical Laboratory Technician or Histotechnician in accordance with New York State Department of Health regulations and possess a License, or be licensure eligible with a limited permit or limited license as a Clinical Laboratory Technician or Histotechnician. Apply online at: http://recruitment.bassett.org/job-opportunities/ To learn more about Bassett Healthcare: http://www.bassett.org/ From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 3 10:13:59 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 3 10:14:16 2012 Subject: [Histonet] RE: Breast IHC testing In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE76B@MCINFRWEM003.ucsfmedicalcenter.org> Remember that you can validate your testing for longer fixation. Run parallel ER/PR/Her2 testing with tissue fixed for various lengths of times and look at the staining correlation between groups. Most likely your longer fixation won't be a problem. If you do see a drop off with longer times you can probably adjust antigen retrieval time to alleviate that. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Fri Feb 3 10:27:38 2012 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Feb 3 10:28:27 2012 Subject: [Histonet] reagents without expiration dates Message-ID: My oldest was from 1965, I can't bit Rene:) Naira ------------------------------ Message: 12 Date: Thu, 2 Feb 2012 13:14:53 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] reagents without expiration dates Ha.Ha,Ha!!!!!!!!!!! I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). Try to beat that!. Ren?? J. From HornHV <@t> archildrens.org Fri Feb 3 10:53:18 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Feb 3 10:53:25 2012 Subject: [Histonet] looking for rita humphrey Message-ID: <25A4DE08332B19499904459F00AAACB719B09F5B92@EVS1.archildrens.org> Rita would you contact me please? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tpodawiltz <@t> lrgh.org Fri Feb 3 10:56:59 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Feb 3 10:57:06 2012 Subject: [Histonet] RE: Breast IHC testing In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE76B@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89F5EE76B@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB6FDFFC@LRGHEXVS1.practice.lrgh.org> This abstract is in the January 2012 edition of CAP Today. Effect of prolonged fixation on evaluation of ER, PR, and HER2 expression in breast cancer Expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 is important in predicting a response to targeted therapies in breast cancer. Therefore, immunohistochemical assays to determine hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) status must be accurate and reproducible. Tissue fixation has been shown to play a crucial role in determining consistency in quality. Although guidelines impose upper limits for the fixation period, the data on which these limits are based are scant. The authors conducted a study to prospectively examine the effect of fixation of longer than 72 hours on these assays. In 101 invasive breast cancer samples, HR and HER2 status were compared between tumor blocks undergoing a short fixation period and those undergoing a period of prolonged fixation. Discordances were classified as an incremental change between categories of (i) a single order of magnitude-that is, a difference in the status of low positive (Allred score, 3) compared with positive (Allred score, 4 to 8) or negative (Allred score, 0 or 2) and vice versa for HRs and a difference in HER2 status of equivocal compared with negative or positive and vice versa or (ii) greater than a single order of magnitude-that is, a difference in the status of positive compared with negative or vice versa. The median fixation time for the short fixation group was 13 hours and 18 minutes (mean, 13 hours and 17 minutes; range, 10 hours and 33 minutes to 17 hours and 45 minutes) and for the prolonged fixation group was 79 hours and 22 minutes (mean, 79 hours and 35 minutes; range, 73 hours and 33 minutes to 102 hours and 30 minutes). Eight cases showed discordances, all of which were of a single order of magnitude, including one for ER, five for PR, and two for HER2. In six of these, a higher score was seen in the prolonged fixation group. The authors concluded that fixation for limited periods beyond 72 hours does not reduce assay sensitivity in determining ER, PR, or HER2 immunohistochemical status. Tong LC, Nelson N, Tsourigiannis J, et al. The effect of prolonged fixation on the immunohistochemical evaluation of estrogen receptor, progesterone receptor, and HER2 expression in invasive breast cancer: a prospective study. Am J Surg Pathol. 2011;35:545-552. Correspondence: Anna Marie Mulligan at mulligana@smh.ca [ Top ] Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -------------------------------------------------------------------------------- Anatomic pathology abstracts editors: Michael Cibull, MD, professor and vice chair, Department of Pathology and Laboratory Medicine, University of Kentucky College of Medicine, Lexington; Rouzan Karabakhtsian, MD, assistant professor of pathology and laboratory medicine, University of Kentucky College of Medicine; and Thomas Cibull, MD, dermatopathologist, Evanston Hospital, NorthShore University HealthSystem, Evanston, Ill. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 03, 2012 11:14 AM To: 'Gale Limron'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Breast IHC testing Remember that you can validate your testing for longer fixation. Run parallel ER/PR/Her2 testing with tissue fixed for various lengths of times and look at the staining correlation between groups. Most likely your longer fixation won't be a problem. If you do see a drop off with longer times you can probably adjust antigen retrieval time to alleviate that. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 3 11:05:05 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 3 11:05:17 2012 Subject: [Histonet] RE: Breast IHC testing In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386324FB6FDFFC@LRGHEXVS1.practice.lrgh.org> References: <8D7C2D242DBD45498006B21122072BF89F5EE76B@MCINFRWEM003.ucsfmedicalcenter.org> <38667E7FB77ECD4E91BFAEB8D986386324FB6FDFFC@LRGHEXVS1.practice.lrgh.org> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE76D@MCINFRWEM003.ucsfmedicalcenter.org> Thanks Tom. Yes, and even much longer fixation has not been shown to adversely affect detection, while short fixation does. The problem is rarely long fixation and is commonly short fixation. Chis van der Loos did a nice study on tonsil with many antibodies showing very short fixation resulted in lower detection than extremely long fixation (one year!). In fact for some epitopes longer fixation seems to be beneficial. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Friday, February 03, 2012 8:57 AM To: Morken, Timothy; 'Gale Limron'; histonet@lists.utsouthwestern.edu Subject: RE: Breast IHC testing This abstract is in the January 2012 edition of CAP Today. Effect of prolonged fixation on evaluation of ER, PR, and HER2 expression in breast cancer Expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 is important in predicting a response to targeted therapies in breast cancer. Therefore, immunohistochemical assays to determine hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) status must be accurate and reproducible. Tissue fixation has been shown to play a crucial role in determining consistency in quality. Although guidelines impose upper limits for the fixation period, the data on which these limits are based are scant. The authors conducted a study to prospectively examine the effect of fixation of longer than 72 hours on these assays. In 101 invasive breast cancer samples, HR and HER2 status were compared between tumor blocks undergoing a short fixation period and those undergoing a period of prolonged fixation. Discordances were classified as an incremental change between categories of (i) a single order of magnitude-that is, a difference in the status of low positive (Allred score, 3) compared with positive (Allred score, 4 to 8) or negative (Allred score, 0 or 2) and vice versa for HRs and a difference in HER2 status of equivocal compared with negative or positive and vice versa or (ii) greater than a single order of magnitude-that is, a difference in the status of positive compared with negative or vice versa. The median fixation time for the short fixation group was 13 hours and 18 minutes (mean, 13 hours and 17 minutes; range, 10 hours and 33 minutes to 17 hours and 45 minutes) and for the prolonged fixation group was 79 hours and 22 minutes (mean, 79 hours and 35 minutes; range, 73 hours and 33 minutes to 102 hours and 30 minutes). Eight cases showed discordances, all of which were of a single order of magnitude, including one for ER, five for PR, and two for HER2. In six of these, a higher score was seen in the prolonged fixation group. The authors concluded that fixation for limited periods beyond 72 hours does not reduce assay sensitivity in determining ER, PR, or HER2 immunohistochemical status. Tong LC, Nelson N, Tsourigiannis J, et al. The effect of prolonged fixation on the immunohistochemical evaluation of estrogen receptor, progesterone receptor, and HER2 expression in invasive breast cancer: a prospective study. Am J Surg Pathol. 2011;35:545-552. Correspondence: Anna Marie Mulligan at mulligana@smh.ca [ Top ] Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -------------------------------------------------------------------------------- Anatomic pathology abstracts editors: Michael Cibull, MD, professor and vice chair, Department of Pathology and Laboratory Medicine, University of Kentucky College of Medicine, Lexington; Rouzan Karabakhtsian, MD, assistant professor of pathology and laboratory medicine, University of Kentucky College of Medicine; and Thomas Cibull, MD, dermatopathologist, Evanston Hospital, NorthShore University HealthSystem, Evanston, Ill. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 03, 2012 11:14 AM To: 'Gale Limron'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Breast IHC testing Remember that you can validate your testing for longer fixation. Run parallel ER/PR/Her2 testing with tissue fixed for various lengths of times and look at the staining correlation between groups. Most likely your longer fixation won't be a problem. If you do see a drop off with longer times you can probably adjust antigen retrieval time to alleviate that. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Melissa.Kuhnla <@t> chsli.org Fri Feb 3 11:32:08 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Feb 3 11:33:00 2012 Subject: [Histonet] Breast IHC testing In-Reply-To: References: Message-ID: Hi, Keep in mind some of the responses I have seem so far are for just IHC. I think there is some literature stating that over fixation affects FISH less, but we perform FISH only and still stay under 48hrs. We use Pathvysion probes and the package insert also recommends not exceeding 48 hours, as signals will fade. We currently have a short step on the processor where tissue is held in 70% alcohol. We have certain specimen collection cut off times so everything is fixed between 6 and 4 hours. Three day, holiday weekends we have technologists come in on Saturday and embed everything. Melissa :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Rcartun <@t> harthosp.org Fri Feb 3 11:51:47 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Feb 3 11:51:57 2012 Subject: [Histonet] Breast IHC testing In-Reply-To: References: Message-ID: <4F2BD863.7400.0077.1@harthosp.org> Those are recommendations from the CAP. You can experiment and then validate longer fixation times. Several papers have come out over the past two years demonstrating little, if any, impact on immunoreactivity for ER, PR, and HER2 when breast tissue has been fixed longer than 72 hours. In my opinion, minimizing cold ischemic time, making sure the tissue does not dry out, and taking "thin" slices of tissue (2-3 mm) for fixation and processing are probably more important than time in formalin. Hopefully, ASCO and CAP will change their recommendations based on these studies. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Gale Limron 2/3/2012 10:24 AM >>> I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Feb 3 11:55:20 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Feb 3 11:55:30 2012 Subject: AW: [Histonet] Breast IHC testing In-Reply-To: References: Message-ID: <8062F9A9A566440EA5CA5F05E00C983C@dielangs.at> Referring to Her2 FISH, we perform this assay also on tissue with longer fixation. We found the protocol with "retrieval" in citratebuffer followed by protease digestion reliable. Also with SISH techniques I prefer long fixation more than underfixation. Is there a chemical explanation, why the fluorochrome will fade after long fixation? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kuhnla, Melissa Gesendet: Freitag, 03. Februar 2012 18:32 An: Gale Limron; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Breast IHC testing Hi, Keep in mind some of the responses I have seem so far are for just IHC. I think there is some literature stating that over fixation affects FISH less, but we perform FISH only and still stay under 48hrs. We use Pathvysion probes and the package insert also recommends not exceeding 48 hours, as signals will fade. We currently have a short step on the processor where tissue is held in 70% alcohol. We have certain specimen collection cut off times so everything is fixed between 6 and 4 hours. Three day, holiday weekends we have technologists come in on Saturday and embed everything. Melissa :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Friday, February 03, 2012 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast IHC testing I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Fri Feb 3 11:58:33 2012 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Feb 3 11:58:39 2012 Subject: [Histonet] Looking for Histology work in Phoenix AZ area Message-ID: <1328291913.82424.YahooMailNeo@web161601.mail.bf1.yahoo.com> Hi all, I am looking for Histology work in the Phoenix area. I am an HT and have 17 years experience with 6 years management. Looking for Full or Part time.. Thanks!! ? Jill Cox, HT ASCP From GaleL <@t> unionhospital.org Fri Feb 3 12:14:56 2012 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Fri Feb 3 12:15:08 2012 Subject: [Histonet] RE: Breast IHC testing Message-ID: Thank you all for your advice and comments. I know this has been discussed before but when I need quick answers I know that I can count on fellow Histonetters for help:) Have a great weekend. Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From Margaret.Perry <@t> sdstate.edu Fri Feb 3 12:50:37 2012 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Feb 3 12:50:42 2012 Subject: [Histonet] picric acid Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From TGoins <@t> mt.gov Fri Feb 3 13:04:00 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Feb 3 13:04:13 2012 Subject: [Histonet] RE: picric acid In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> Message-ID: The picric acid around the cap would not be "1%". The acetone is long gone. Wipe the threads or pipette the reagent from the bottle. Our chemical safety office informed us that friction from removing a cap can be enough to set it off. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, February 03, 2012 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] picric acid I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Feb 3 13:08:59 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri Feb 3 13:09:04 2012 Subject: [Histonet] RE: picric acid In-Reply-To: References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DDC@PHSXMB30.partners.org> I concur. The problem is the dried picric acid. We used it so infrequently and had a large bottle, that I had Safety dispose of it. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, February 03, 2012 2:04 PM To: Perry, Margaret; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: picric acid The picric acid around the cap would not be "1%". The acetone is long gone. Wipe the threads or pipette the reagent from the bottle. Our chemical safety office informed us that friction from removing a cap can be enough to set it off. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, February 03, 2012 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] picric acid I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mcauliff <@t> umdnj.edu Fri Feb 3 13:16:50 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 3 13:15:47 2012 Subject: [Histonet] RE: picric acid In-Reply-To: References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> Message-ID: <4F2C32A2.4060305@umdnj.edu> "Our chemical safety office informed us that friction from removing a cap can be enough to set it off." I have heard this for many, many years but has there ever been a case of such a thing happening? Of course we should be cautions and keep our picric acid wet but ... I have seen news clips in which the bomb squad packs explosives around the picric acid (from an old high school lab) out in a field and sets it off. BOOM! Sure, the picric acid was surrounded by explosives. Geoff On 2/3/2012 2:04 PM, Goins, Tresa wrote: > The picric acid around the cap would not be "1%". The acetone is long gone. Wipe the threads or pipette the reagent from the bottle. > Our chemical safety office informed us that friction from removing a cap can be enough to set it off. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret > Sent: Friday, February 03, 2012 11:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] picric acid > > I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From vtobias <@t> uw.edu Fri Feb 3 13:22:42 2012 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri Feb 3 13:24:09 2012 Subject: [Histonet] RE: picric acid In-Reply-To: <4F2C32A2.4060305@umdnj.edu> References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> <4F2C32A2.4060305@umdnj.edu> Message-ID: I vaguely remember reading about placing the container underwater to moisten the threads and then the lid could be removed. If you have any doubt have it disposed. Always better to be safe than sorry. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, February 03, 2012 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: picric acid "Our chemical safety office informed us that friction from removing a cap can be enough to set it off." I have heard this for many, many years but has there ever been a case of such a thing happening? Of course we should be cautions and keep our picric acid wet but ... I have seen news clips in which the bomb squad packs explosives around the picric acid (from an old high school lab) out in a field and sets it off. BOOM! Sure, the picric acid was surrounded by explosives. Geoff On 2/3/2012 2:04 PM, Goins, Tresa wrote: > The picric acid around the cap would not be "1%". The acetone is long gone. Wipe the threads or pipette the reagent from the bottle. > Our chemical safety office informed us that friction from removing a cap can be enough to set it off. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret > Sent: Friday, February 03, 2012 11:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] picric acid > > I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 3 13:27:15 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 3 13:27:20 2012 Subject: [Histonet] picric acid In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> Message-ID: <1328297235.95970.YahooMailClassic@web162103.mail.bf1.yahoo.com> There have been cases where the rotary valve of VIPs have been damaged after using Bouin's fixative without proper washing. On the other hand I used to keep picric acid but always in saturated solution, which is about 1%. As long as you have water?along with?the picric acid bottle,?there is no problem. Ren? J.? --- On Fri, 2/3/12, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] picric acid To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 3, 2012, 1:50 PM I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and? Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> CSILaboratories.com Fri Feb 3 13:40:12 2012 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Feb 3 13:40:17 2012 Subject: [Histonet] picric acid In-Reply-To: <1328297235.95970.YahooMailClassic@web162103.mail.bf1.yahoo.com> References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> <1328297235.95970.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: <467983BDAC5880438E2202F6B9BF79A832FE42@exchange-be-01.CSI-LABS.local> The explosion I witnessed was initiated by a blasting cap. The bomb squad director said it exploded with the force of several sticks of dynamite. If anyone has picric acid still in their lab, exercise GREAT caution. Mark Turner, HT(ASCP) QIHC IHC / Histology Manager 678-319-3321 Direct 770-508-7644 Cell??????????????????????? 678-319-1454 mailto:mturner@csilaboratories.com csilaboratories.com 2580 Westside Parkway Alpharetta, GA 30004 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 03, 2012 2:27 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry Subject: Re: [Histonet] picric acid There have been cases where the rotary valve of VIPs have been damaged after using Bouin's fixative without proper washing. On the other hand I used to keep picric acid but always in saturated solution, which is about 1%. As long as you have water?along with?the picric acid bottle,?there is no problem. Ren? J.? --- On Fri, 2/3/12, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] picric acid To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 3, 2012, 1:50 PM I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and? Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Fri Feb 3 14:40:47 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Feb 3 14:40:51 2012 Subject: [Histonet] picric acid In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> Message-ID: I do not know about a solution, but a REALLY BIG one with a 1 oz bottle of picric acid crystals. When I was in college bottle was vibrated off a shelf by an out of balance centrifuge. It was common for students to work w/ chemicals late at night when taking inorganic chem. The student loaded the centrifuge and left the room (went outside for a smoke), bottle dropped to the floor, exploded and left a 6 ft x 8 ft whole in the counter and wall and pretty much destroyed the 30 ft x 30 ft lab. Very lucky no one was hurt. At the time, i remember thinking, hey we will get a pass on the next assignment, only got two days off. University Safety team had an accurate listing of all chemicals on the shelves and determined it had to be the picric acid. Safety and the Fire marshal did a sweep of the university and found six other bottles in various labs on campus. Never did hear how they disposed and I bet that made a BIB BANG!! William DeSalvo, B.S., HTL(ASCP) > From: Margaret.Perry@sdstate.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 3 Feb 2012 18:50:37 +0000 > Subject: [Histonet] picric acid > > I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Fri Feb 3 15:08:27 2012 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Feb 3 15:08:35 2012 Subject: [Histonet] picric acid In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> Message-ID: <4F2C4CCB.6040905@vneubert.com> Just wipe away any drops after closing the bottle. But your solution makes nice shiny plastic objects become ugly dull yellow plastic objects. Avoid spilling ;) > I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Feb 3 15:12:42 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 3 15:12:45 2012 Subject: [Histonet] PA area folks--company to NOT use Message-ID: Hello histonetters! I am having a great deal of trouble getting a microscope cleaning bill paid. We were overcharged by an hour. I suggest if you ever need your instruments cleaned or repaired, DO NOT USE GEORGE NABLE INSTRUMENTS. He has consistently overcharged us for his work, even after writing down a certain price on a purchase order. Just a warning. He works in the Pittsburgh area. I wish there was a yelp for scientists where I could post this, because I know a lot of people use him. Emily Sours The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From TGoins <@t> mt.gov Fri Feb 3 15:38:22 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Feb 3 15:38:31 2012 Subject: [Histonet] RE: picric acid In-Reply-To: <4F2C32A2.4060305@umdnj.edu> References: <25F4FBA34BE9D142964ECC4525B82AEE02CD1D@SDSU-EX03.jacks.local> <4F2C32A2.4060305@umdnj.edu> Message-ID: Geoff - Yes - see if you can get the footage of the damage done in a lab in Idaho. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, February 03, 2012 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: picric acid "Our chemical safety office informed us that friction from removing a cap can be enough to set it off." I have heard this for many, many years but has there ever been a case of such a thing happening? Of course we should be cautions and keep our picric acid wet but ... I have seen news clips in which the bomb squad packs explosives around the picric acid (from an old high school lab) out in a field and sets it off. BOOM! Sure, the picric acid was surrounded by explosives. Geoff On 2/3/2012 2:04 PM, Goins, Tresa wrote: > The picric acid around the cap would not be "1%". The acetone is long gone. Wipe the threads or pipette the reagent from the bottle. > Our chemical safety office informed us that friction from removing a cap can be enough to set it off. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, > Margaret > Sent: Friday, February 03, 2012 11:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] picric acid > > I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services Box 2175 South Dakota > State University Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Fri Feb 3 16:17:55 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Feb 3 16:18:01 2012 Subject: [Histonet] RE: Breast CAP requirements In-Reply-To: <20120203180249.1A96463E8D4@mx10.myoutlookonline.com> References: <20120203180249.1A96463E8D4@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB5127B@S10MAILD001N1.SH10.lan> We have a tech come in on the weekends to embed so the fixation never exceeds 48 hours. We keep a log to record the fixation times of all our breast cases. For needle core biopsies if the client has written the time the specimen was taken on the requisition, we record that time otherwise it is the time we received it in the lab. With breast lumpectomies or mastectomies the fixation time starts once the specimen has been grossed and blocked, not before. With each report we have a blurb stating the fixation time of the specimen. It goes something like: 'The specimen has been fixed for a minimum of 12 hours to a maximum of 48 hours in accordance with CAP breast fixation guidelines for Her2Neu testing'. Sometimes though, courier delivery causes delays and its already been over 48 hours when we receive them in the lab. For instance a client does a breast needle core biopsy on a Friday but we do not receive the specimen until the following Monday. In these instances we record on the report that the specimen has had greater than 48 hours fixation. We do not do the Her2Neu testing in house and when we send them out for testing we have to record on the outside consultants requisition the fixation time. Sometimes its impossible not to go over the 48 hours and when it happens we just record it in the report. Joanne Clark, AAS,HT(ASCP) Histology Supervisor Pathology Consultants of New Mexico ---------------------------------------------------------------------- Message: 1 Date: Fri, 03 Feb 2012 12:51:47 -0500 From: "Richard Cartun" Subject: Re: [Histonet] Breast IHC testing To: "histonet@lists.utsouthwestern.edu" , "Gale Limron" Message-ID: <4F2BD863.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Those are recommendations from the CAP. You can experiment and then validate longer fixation times. Several papers have come out over the past two years demonstrating little, if any, impact on immunoreactivity for ER, PR, and HER2 when breast tissue has been fixed longer than 72 hours. In my opinion, minimizing cold ischemic time, making sure the tissue does not dry out, and taking "thin" slices of tissue (2-3 mm) for fixation and processing are probably more important than time in formalin. Hopefully, ASCO and CAP will change their recommendations based on these studies. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Gale Limron 2/3/2012 10:24 AM >>> I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.Hoy <@t> UTSouthwestern.edu Fri Feb 3 19:25:59 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Fri Feb 3 19:26:05 2012 Subject: [Histonet] picric acid In-Reply-To: <2f269cdda3c64e27ba2f2bdde1badf36@SWMSHUB3.swmed.org> Message-ID: Way back in 1976, I had just been disgorged from graduate school with a MS degree in microbiology, and I landed a job in a hospital lab in a Chicago suburb as the micro supervisor. Since I was the new guy, and no one else wanted the (unpaid) job, I was also appointed as Laboratory Safety Officer. One morning I sallied forth into the histology lab with my clipboard and flashlight to look for safety hazards. Everything was in good shape until I looked under a sink. There was a brown glass gallon bottle at the back of the cabinet, which I dragged out and plunked down on the bench. The label was yellow with age (and the pigment of picric acid which had leaked from a small crack in the bottle.) The label identified the contents as liquid picric acid, which was now a single solid crystal, since all of the liquid had evaporated. It would have been about a half gallon if it had still been liquid. I recalled my clinical chemistry class, in which we learned that the picric acid we used for serum creatinine was explosive in the crystalline state. I called the local fire department, and they were first concerned that we had suffered an acid spill, but I explained that this acid was a solid, but potentially explosive. Since there was no chemical spill, they were not too concerned, and said they would get back to me. About an hour later, the bomb squad showed up in full regalia. The fire department had looked up picric acid and found it was 2,4,6 trinitrophenol, a close relative of 2,4,6 trinitrotoluene (TNT). They evacuated that wing of the hospital (the entire lab and about 50 patients on the two floors above the lab), and carried the bottle of picric acid out in their bomb disposal device. They detonated it in a field far away from the hospital by firing a rifle shot into it. It left a crater about 20 feet in diameter and ten feet deep. It was featured on the evening news by at least two of the Chicago TV stations. They had nice video of patients on gurneys being rolled down the halls, and a great view of the exploding bottle. Mythbusters could have learned from that video. Unfortunately, the hospital administration was not amused by the publicity, and we had to explain to multiple committees why we had such a hazardous substance in the lab. The final comment on this incident is that the bottle had been under the sink for years. No one working in the lab at that time could remember when it was last used. This cabinet was where the histotechs stored their purses (back in the days when nearly all histotechs were female). They would come in at the beginning of their shift and toss (literally) their purses into the cabinet. Virchow be praised, they had never hit the bottle with enough force to detonate it. If any of the histotechs who worked at HPH back in those days are on this list, I'd love to hear from you. Best regards, Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 2/3/12 12:50 PM, "Perry, Margaret" wrote: > I am curious how big an explosion there would be from 1% picric acid in > acetone if a little dried around the cap. From jshea121 <@t> roadrunner.com Fri Feb 3 19:45:30 2012 From: jshea121 <@t> roadrunner.com (Shea's) Date: Fri Feb 3 19:45:42 2012 Subject: [Histonet] Re:Breast IHC testing In-Reply-To: <3C.D4.01905.FA02C2F4@cdptpa-mxlb.mail.rr.com> References: <3C.D4.01905.FA02C2F4@cdptpa-mxlb.mail.rr.com> Message-ID: <8472F06FFFEB4CDE900F6DD326BE6E48@JoannePC> We have the same "weekend dilemma" with greater than 48 hour fixation for breast tissue for Her-2. We send them to a huge reference lab that stated exactly as Richard. They have found that 72 hours has little impact on ER/PR/HER2 , however, we do request HER2 - FISH (instead of IHC) in these cases. Furthermore, the ref lab said that some of the things that labs are doing to avoid over fixation are worse (such as holding tissue in alcohol for extended periods of time, etc) because that hasn't been validated. Also, I spoke with the radiologists that perform the core needle bxs and stereotactic and they try to avoid sched. them before a weekend or holiday. The excisional bx performed in the OR usually already have ER/PR/HER2 on the previous needle bx. Jan -------------------------------------------------- From: Sent: Friday, February 03, 2012 1:00 PM To: Subject: Histonet Digest, Vol 99, Issue 5 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Breast IHC testing (Richard Cartun) > 2. AW: [Histonet] Breast IHC testing (Gudrun Lang) > 3. Looking for Histology work in Phoenix AZ area (Jill Cox) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 03 Feb 2012 12:51:47 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] Breast IHC testing > To: "histonet@lists.utsouthwestern.edu" > , "Gale Limron" > > Message-ID: <4F2BD863.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Those are recommendations from the CAP. You can experiment and then > validate longer fixation times. Several papers have come out over the > past two years demonstrating little, if any, impact on immunoreactivity > for ER, PR, and HER2 when breast tissue has been fixed longer than 72 > hours. In my opinion, minimizing cold ischemic time, making sure the > tissue does not dry out, and taking "thin" slices of tissue (2-3 mm) for > fixation and processing are probably more important than time in formalin. > Hopefully, ASCO and CAP will change their recommendations based on these > studies. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> Gale Limron 2/3/2012 10:24 AM >>> > I would like to know what other hospitals are doing with breast specimens > that are resected on Friday and are in formalin longer than the maximum > number of hours that CAP allows for ER/PR and HER2/neu testing. We are > running into this problem since we don't currently work Saturday hours. > Thank you! > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it is > addressed and may contain information that is privileged, confidential or > otherwise protected from disclosure. Dissemination, distribution or > copying of this e-mail or the information herein by anyone other than the > intended recipient, or an employee or agent responsible for delivering the > message to the intended recipient, is prohibited. If you received this > message in error, please delete without copying and kindly e-mail a reply > to inform us of the mistake in delivery. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Fri, 3 Feb 2012 18:55:20 +0100 > From: "Gudrun Lang" > Subject: AW: [Histonet] Breast IHC testing > To: "'Kuhnla, Melissa'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <8062F9A9A566440EA5CA5F05E00C983C@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Referring to Her2 FISH, we perform this assay also on tissue with longer > fixation. We found the protocol with "retrieval" in citratebuffer followed > by protease digestion reliable. > Also with SISH techniques I prefer long fixation more than underfixation. > > Is there a chemical explanation, why the fluorochrome will fade after long > fixation? > > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kuhnla, > Melissa > Gesendet: Freitag, 03. Februar 2012 18:32 > An: Gale Limron; histonet@lists.utsouthwestern.edu > Betreff: RE: [Histonet] Breast IHC testing > > Hi, > Keep in mind some of the responses I have seem so far are for just IHC. > I think there is some literature stating that over fixation affects FISH > less, but we perform FISH only and still stay under 48hrs. We use > Pathvysion probes and the package insert also recommends not exceeding > 48 hours, as signals will fade. > We currently have a short step on the processor where tissue is held in > 70% alcohol. We have certain specimen collection cut off times so > everything is fixed between 6 and 4 hours. Three day, holiday weekends > we have technologists come in on Saturday and embed everything. > Melissa :) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale > Limron > Sent: Friday, February 03, 2012 10:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breast IHC testing > > I would like to know what other hospitals are doing with breast > specimens that are resected on Friday and are in formalin longer than > the maximum number of hours that CAP allows for ER/PR and HER2/neu > testing. We are running into this problem since we don't currently work > Saturday hours. > Thank you! > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it is > addressed and may contain information that is privileged, confidential > or otherwise protected from disclosure. Dissemination, distribution or > copying of this e-mail or the information herein by anyone other than > the intended recipient, or an employee or agent responsible for > delivering the message to the intended recipient, is prohibited. If you > received this message in error, please delete without copying and kindly > e-mail a reply to inform us of the mistake in delivery. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail, and any attachments therein, is > confidential > and for use by the intended addressee only. If this message is received by > you in error please do not disseminate or read further. Please reply to > the > sender that you have received the message in error, then delete the > message. > Although Catholic Health Services of Long Island attempts to sweep e-mail > and attachments for viruses, it does not guarantee that either are > virus-free and accepts no liability for any damage sustained as a result > of > viruses. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Fri, 3 Feb 2012 09:58:33 -0800 (PST) > From: Jill Cox > Subject: [Histonet] Looking for Histology work in Phoenix AZ area > To: "Histonet@Lists. Edu" > Message-ID: > <1328291913.82424.YahooMailNeo@web161601.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi all, > I am looking for Histology work in the Phoenix area. I am an HT and have > 17 years experience with 6 years management. Looking for Full or Part > time.. Thanks!! > > Jill Cox, HT ASCP > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 5 > *************************************** > From macveigh <@t> usc.edu Fri Feb 3 20:51:47 2012 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Feb 3 20:51:49 2012 Subject: [Histonet] Equipment available to the highest bidder Message-ID: <008301cce2e7$efde7c80$cf9b7580$@usc.edu> Hi list, On 21 of February we will have the following equipment available to the highest bidder: 1. Tissue processor - Tissue Tek VIP-E (still in use) 2. Tissue processor - SHANDON Citadel 2000 One paraffin tank missing 3. JUNG AUTOSTAINER LX (still in use) By the end of the month we will also have available 4. Embedding center - SHANDON Histocentre2 (still in use) If interested please send me an offer. Michelle Aloni MS HTL Research Specialist USC Keck School of Medicine Los Angeles, CA From rjbuesa <@t> yahoo.com Sat Feb 4 11:12:26 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 4 11:12:30 2012 Subject: [Histonet] PA area folks--company to NOT use In-Reply-To: Message-ID: <1328375546.96714.YahooMailClassic@web162105.mail.bf1.yahoo.com> You should also?file a complaint with the Better Business Bureau of your area. Ren? J. --- On Fri, 2/3/12, Emily Sours wrote: From: Emily Sours Subject: [Histonet] PA area folks--company to NOT use To: histonet@lists.utsouthwestern.edu Date: Friday, February 3, 2012, 4:12 PM Hello histonetters! I am having a great deal of trouble getting a microscope cleaning bill paid.? We were overcharged by an hour.? I suggest if you ever need your instruments cleaned or repaired, DO NOT USE GEORGE NABLE INSTRUMENTS.? He has consistently overcharged us for his work, even after writing down a certain price on a purchase order. Just a warning.? He works in the Pittsburgh area. I wish there was a yelp for scientists where I could post this, because I know? a lot of people use him. Emily? Sours The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Sat Feb 4 22:53:14 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Feb 4 22:53:22 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 3 In-Reply-To: <4f2b1910.234cec0a.7b7a.ffffb33bSMTPIN_ADDED@mx.google.com> References: <4f2b1910.234cec0a.7b7a.ffffb33bSMTPIN_ADDED@mx.google.com> Message-ID: Kathryn Stoll, Did you freeze & thaw your -20c antibody? The freeze & thaw can cause antibody degradation (ie. start with 1:100 to 1:20 with few thawing). Have you consider switching storage antibodies from -20c to 4c? Innovex (Berkeley, Ca) sell antibody diluent for this purpose. Madeleine Huey, HT (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Thu, Feb 2, 2012 at 3:15 PM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Topoisomerase I (Stoll, Kathryn) > ? 2. EGFR ISH Ventana ?(Zheng, Mei) > ? 3. reagents without expiration dates (Stoll, Kathryn) > ? 4. RE: reagents without expiration dates (Goins, Tresa) > ? 5. RE: reagents without expiration dates (WILLIAM DESALVO) > ? 6. Re: ?slides for frozens (Sherwood, Margaret) > ? 7. Re: ?slides for frozens (Rene J Buesa) > ? 8. RE: slides for frozens (Sherwood, Margaret) > ? 9. RE: slides for frozens (Rene J Buesa) > ?10. Re: reagents without expiration dates (Emily Sours) > ?11. histotech needed (Horn, Hazel V) > ?12. Re: reagents without expiration dates (Rene J Buesa) > ?13. Re: RE: Interviewing Histotechs... (Joe Nocito) > ?14. RE: reagents without expiration dates (Goins, Tresa) > ?15. RE: reagents without expiration dates (WILLIAM DESALVO) > ?16. RE: reagents without expiration dates (Jennifer MacDonald) > ?17. Re: AW: [Histonet] C4d IF on FFPE kidney (Tony Reilly) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 2 Feb 2012 12:16:35 -0600 > From: "Stoll, Kathryn" > Subject: [Histonet] Topoisomerase I > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<110E7925E2B91945A9B79EDFD0DC2B34EB103BAD63@MCWMBX2.mcwcorp.net> > Content-Type: text/plain; charset="us-ascii" > > I am using TOP1 that originally worked at 1:100 dilution. ?I am now using at 1:20. > Any ideas why the staining has decreased so much? ?I have it stored in -20 in aliquots. > > Or has someone found a TOP1 that works for them. > > It is too expensive to run at 1:20. ?Thanks, > > Kathryn Stoll, HT(ASCP) > Depatment of Pathology > Medical College of Wisconsin > 9200 W Wisconsin Ave > Milwaukee WI 53226 > 414.805.1525 > kstoll@mcw.edu > > > > ------------------------------ > > Message: 2 > Date: Thu, 2 Feb 2012 13:51:10 -0500 > From: "Zheng, Mei" > Subject: [Histonet] EGFR ISH Ventana > To: > Message-ID: > ? ? ? ?<7C58021620332F438692F2B4EFF5782703C56A3A@PHSXMB26.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi > > Can someone help me with the EGFR ISH protocol for Ventana? > > Thanks! > > Mei Zheng, HTL(ASCP) QIHC > Clinical Supervisor > Immunohistochemistry Lab. > Department of Pathology > Brigham and Women's Hospital > Boston, MA 02115 > Lab Tel: 617 732-7790 > > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > ------------------------------ > > Message: 3 > Date: Thu, 2 Feb 2012 12:56:58 -0600 > From: "Stoll, Kathryn" > Subject: [Histonet] reagents without expiration dates > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<110E7925E2B91945A9B79EDFD0DC2B34EB103BAE00@MCWMBX2.mcwcorp.net> > Content-Type: text/plain; charset="us-ascii" > > Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? > CAP checklist ANP 21382 > > Thanks, > Kathryn Stoll, HT(ASCP) > Depatment of Pathology > Medical College of Wisconsin > 9200 W Wisconsin Ave > Milwaukee WI 53226 > 414.805.1525 > kstoll@mcw.edu > > > > ------------------------------ > > Message: 4 > Date: Thu, 2 Feb 2012 19:04:03 +0000 > From: "Goins, Tresa" > Subject: [Histonet] RE: reagents without expiration dates > To: "Stoll, Kathryn" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > A general rule that I am familiar with is a maximum shelf-life of 1 year, basically due to repetitive access leading to eventual contamination. > > > Tresa Goins > Veterinary Diagnostic Lab > South 19th and Lincoln > Bozeman, MT 59718 > 406-994-6353?- phone > 406-994-6344?- fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stoll, Kathryn > Sent: Thursday, February 02, 2012 11:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] reagents without expiration dates > > Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? > CAP checklist ANP 21382 > > Thanks, > Kathryn Stoll, HT(ASCP) > Depatment of Pathology > Medical College of Wisconsin > 9200 W Wisconsin Ave > Milwaukee WI 53226 > 414.805.1525 > kstoll@mcw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Thu, 2 Feb 2012 12:07:31 -0700 > From: WILLIAM DESALVO > Subject: RE: [Histonet] reagents without expiration dates > To: , histonet > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Per SOP, we relabel, list date of receipt, test for quality and then apply a 12 month expiration date. We re-test after 12 months and continue to use, with 12 month dating, as long as the reagent meets quality standards set in the SOP. > > William DeSalvo, B.S., HTL(ASCP) > > > >> From: kstoll@mcw.edu >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 2 Feb 2012 12:56:58 -0600 >> Subject: [Histonet] reagents without expiration dates >> >> Could anyone share a policy to deal with regents that do not have a manufacturer's expiration date? >> CAP checklist ANP 21382 >> >> Thanks, >> Kathryn Stoll, HT(ASCP) >> Depatment of Pathology >> Medical College of Wisconsin >> 9200 W Wisconsin Ave >> Milwaukee WI 53226 >> 414.805.1525 >> kstoll@mcw.edu >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 6 > Date: Thu, 2 Feb 2012 14:46:40 -0500 > From: "Sherwood, Margaret" > Subject: Re: ?[Histonet] slides for frozens > To: "histonet" > Message-ID: > ? ? ? ?<073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCD@PHSXMB30.partners.org> > Content-Type: text/plain; charset="us-ascii" > > To all: > > We have been using the same slides for paraffins and frozens. ?We have no > trouble with paraffin-embedded tissue adhering to the slides through the whole > staining procedure. ?But our frozens (even 5um sections)have been falling off > during manual staining. ?The slides are (+) charged. ?We used to use slides from > Fisher, but they are so expensive, we changed to an independent vendor (half the > price). > > Our procedure for frozen H&Es has recently changed: > 1. Fix in 95% ETOH ? ? ? 1 min. > 2. Hematoxylin ? ? ? ? ? 5 min. > 3. Acetic acid H2O ? ? ? 2 quick dips > 4. Wash-running H2O ? ? ?10 seconds (10 dips) > 5. Bluing reagent ? ? ? ? ? ? ?10 dips > 6. Wash-running H2O ? ? ?10 seconds (or 10 dips) > 7. Eosin ? ? ? ? ? ? ? ? ? ? 5 seconds > 8. Dehydrate-95% ETOH ? ?10 dips each > 9. Dehydrate-100%ETOH ? ?10 dips each > 10.CitriSolv ? ? ? ? ? ? 10 dips each > 11.Coverslip > > The tissue seems to fall off in the bluing reagent (we order from Mossberg). > > Can anyone help us? ?Suggest anything--what slides you are using? ?We have had > the same problem with special stains (i.e. Oil Red O for fat on frozens). > > Thanks! > Peggy > > P.S. ?I use the same slides for my 1um plastic embedding which I dip in water > rinses and have no problem with the tissue falling off. > > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > > > ------------------------------ > > Message: 7 > Date: Thu, 2 Feb 2012 11:56:51 -0800 (PST) > From: Rene J Buesa > Subject: Re: ?[Histonet] slides for frozens > To: histonet , ? ? ? MargaretSherwood > ? ? ? ? > Message-ID: > ? ? ? ?<1328212611.97248.YahooMailClassic@web162102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > There is a known saying that goes: "You get what you pay for". > Ren? J. > > --- On Thu, 2/2/12, Sherwood, Margaret wrote: > > > From: Sherwood, Margaret > Subject: Re: [Histonet] slides for frozens > To: "histonet" > Date: Thursday, February 2, 2012, 2:46 PM > > > To all: > > We have been using the same slides for paraffins and frozens.? We have no > trouble with paraffin-embedded tissue adhering to the slides through the whole > staining procedure.? But our frozens (even 5um sections)have been falling off > during manual staining.? The slides are (+) charged.? We used to use slides from > Fisher, but they are so expensive, we changed to an independent vendor (half the > price). > > Our procedure for frozen H&Es has recently changed: > 1. Fix in 95% ETOH? ? ???1 min. > 2. Hematoxylin? ? ? ? ???5 min. > 3. Acetic acid H2O?????2 quick dips > 4. Wash-running H2O? ? ? 10 seconds (10 dips) > 5. Bluing reagent??? ? ? ???10 dips > 6. Wash-running H2O? ? ? 10 seconds (or 10 dips) > 7. Eosin??? ? ? ? ? ? ???5 seconds > 8. Dehydrate-95% ETOH? ? 10 dips each > 9. Dehydrate-100%ETOH? ? 10 dips each > 10.CitriSolv? ? ? ? ? ???10 dips each > 11.Coverslip > > The tissue seems to fall off in the bluing reagent (we order from Mossberg). > > Can anyone help us?? Suggest anything--what slides you are using?? We have had > the same problem with special stains (i.e. Oil Red O for fat on frozens). > > Thanks! > Peggy > > P.S.? I use the same slides for my 1um plastic embedding which I dip in water > rinses and have no problem with the tissue falling off. > > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 8 > Date: Thu, 2 Feb 2012 15:03:35 -0500 > From: "Sherwood, Margaret" > Subject: RE: [Histonet] slides for frozens > To: "Rene J Buesa" , "histonet" > ? ? ? ? > Message-ID: > ? ? ? ?<073AE2BEA1C2BA4A8837AB6C4B943D9708DB5DCE@PHSXMB30.partners.org> > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > That may be true, but I don't recall having this problem with our previous > protocol (which was longer and included a fix in alcoholic formalin). ?I think > we will try the "old" method and see what happens. > > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > ________________________________ > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, February 02, 2012 2:57 PM > To: histonet; Sherwood, Margaret > Subject: Re: [Histonet] slides for frozens > > > There is a known saying that goes: "You get what you pay for". > Ren? J. > > --- On Thu, 2/2/12, Sherwood, Margaret wrote: > > > > ? ? ? ?From: Sherwood, Margaret > ? ? ? ?Subject: Re: [Histonet] slides for frozens > ? ? ? ?To: "histonet" > ? ? ? ?Date: Thursday, February 2, 2012, 2:46 PM > > > ? ? ? ?To all: > > ? ? ? ?We have been using the same slides for paraffins and frozens. ?We have > no > ? ? ? ?trouble with paraffin-embedded tissue adhering to the slides through the > whole > ? ? ? ?staining procedure. ?But our frozens (even 5um sections)have been > falling off > ? ? ? ?during manual staining. ?The slides are (+) charged. ?We used to use > slides from > ? ? ? ?Fisher, but they are so expensive, we changed to an independent vendor > (half the > ? ? ? ?price). > > ? ? ? ?Our procedure for frozen H&Es has recently changed: > ? ? ? ?1. Fix in 95% ETOH ? ? ? 1 min. > ? ? ? ?2. Hematoxylin ? ? ? ? ? 5 min. > ? ? ? ?3. Acetic acid H2O ? ? 2 quick dips > ? ? ? ?4. Wash-running H2O ? ? ?10 seconds (10 dips) > ? ? ? ?5. Bluing reagent ? ? ? ? ? 10 dips > ? ? ? ?6. Wash-running H2O ? ? ?10 seconds (or 10 dips) > ? ? ? ?7. Eosin ? ? ? ? ? ? ? ? 5 seconds > ? ? ? ?8. Dehydrate-95% ETOH ? ?10 dips each > ? ? ? ?9. Dehydrate-100%ETOH ? ?10 dips each > ? ? ? ?10.CitriSolv ? ? ? ? ? ? 10 dips each > ? ? ? ?11.Coverslip > > ? ? ? ?The tissue seems to fall off in the bluing reagent (we order from > Mossberg). > > ? ? ? ?Can anyone help us? ?Suggest anything--what slides you are using? ?We > have had > ? ? ? ?the same problem with special stains (i.e. Oil Red O for fat on > frozens). > > ? ? ? ?Thanks! > ? ? ? ?Peggy > > ? ? ? ?P.S. ?I use the same slides for my 1um plastic embedding which I dip in > water > ? ? ? ?rinses and have no problem with the tissue falling off. > > > ? ? ? ?Peggy Sherwood > ? ? ? ?Lab Associate, Photopathology > ? ? ? ?Wellman Center for Photomedicine (EDR 214) > ? ? ? ?Massachusetts General Hospital > ? ? ? ?50 Blossom Street > ? ? ? ?Boston, MA 02114-2696 > ? ? ? ?617-724-4839 (voice mail) > ? ? ? ?617-726-6983 (lab) > ? ? ? ?617-726-1206 (fax) > ? ? ? ?msherwood@partners.org > > > > > ? ? ? ?The information in this e-mail is intended only for the person to whom > it is > ? ? ? ?addressed. If you believe this e-mail was sent to you in error and the > e-mail > ? ? ? ?contains patient information, please contact the Partners Compliance > HelpLine at > ? ? ? ?http://www.partners.org/complianceline . If the e-mail was sent to you > in error > ? ? ? ?but does not contain patient information, please contact the sender and > properly > ? ? ? ?dispose of the e-mail. > > > ? ? ? ?_______________________________________________ > ? ? ? ?Histonet mailing list > ? ? ? ?Histonet@lists.utsouthwestern.edu > > > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 9 > Date: Thu, 2 Feb 2012 12:08:02 -0800 (PST) > From: Rene J Buesa > Subject: RE: [Histonet] slides for frozens > To: MargaretSherwood > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ?<1328213282.15651.YahooMailClassic@web162102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That could be the solution. The problem is that you changed 2 things: the protocol and the slides and now you do not know which is "the culprit". Returning to the old protocols is a first step to trying to solve the present situation and, as you can imagine, if the old protocol does not solve the problem, the slides may be the cause. > Ren? J. > > --- On Thu, 2/2/12, Sherwood, Margaret wrote: > > > From: Sherwood, Margaret > Subject: RE: [Histonet] slides for frozens > To: "Rene J Buesa" , "histonet" > Date: Thursday, February 2, 2012, 3:03 PM > > > That may be true, but I don't recall having this problem with our previous > protocol (which was longer and included a fix in alcoholic formalin).? I think > we will try the "old" method and see what happens. > > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > ________________________________ > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, February 02, 2012 2:57 PM > To: histonet; Sherwood, Margaret > Subject: Re: [Histonet] slides for frozens > > > There is a known saying that goes: "You get what you pay for". > Ren? J. > > --- On Thu, 2/2/12, Sherwood, Margaret wrote: > > > > ??? From: Sherwood, Margaret > ??? Subject: Re: [Histonet] slides for frozens > ??? To: "histonet" > ??? Date: Thursday, February 2, 2012, 2:46 PM > > > ??? To all: > > ??? We have been using the same slides for paraffins and frozens.? We have > no > ??? trouble with paraffin-embedded tissue adhering to the slides through the > whole > ??? staining procedure.? But our frozens (even 5um sections)have been > falling off > ??? during manual staining.? The slides are (+) charged.? We used to use > slides from > ??? Fisher, but they are so expensive, we changed to an independent vendor > (half the > ??? price). > > ??? Our procedure for frozen H&Es has recently changed: > ??? 1. Fix in 95% ETOH? ? ???1 min. > ??? 2. Hematoxylin? ? ? ? ???5 min. > ??? 3. Acetic acid H2O? ???2 quick dips > ??? 4. Wash-running H2O? ? ? 10 seconds (10 dips) > ??? 5. Bluing reagent? ? ? ? ???10 dips > ??? 6. Wash-running H2O? ? ? 10 seconds (or 10 dips) > ??? 7. Eosin? ? ? ? ? ? ? ???5 seconds > ??? 8. Dehydrate-95% ETOH? ? 10 dips each > ??? 9. Dehydrate-100%ETOH? ? 10 dips each > ??? 10.CitriSolv? ? ? ? ? ???10 dips each > ??? 11.Coverslip > > ??? The tissue seems to fall off in the bluing reagent (we order from > Mossberg). > > ??? Can anyone help us?? Suggest anything--what slides you are using?? We > have had > ??? the same problem with special stains (i.e. Oil Red O for fat on > frozens). > > ??? Thanks! > ??? Peggy > > ??? P.S.? I use the same slides for my 1um plastic embedding which I dip in > water > ??? rinses and have no problem with the tissue falling off. > > > ??? Peggy Sherwood > ??? Lab Associate, Photopathology > ??? Wellman Center for Photomedicine (EDR 214) > ??? Massachusetts General Hospital > ??? 50 Blossom Street > ??? Boston, MA 02114-2696 > ??? 617-724-4839 (voice mail) > ??? 617-726-6983 (lab) > ??? 617-726-1206 (fax) > ??? msherwood@partners.org > > > > > ??? The information in this e-mail is intended only for the person to whom > it is > ??? addressed. If you believe this e-mail was sent to you in error and the > e-mail > ??? contains patient information, please contact the Partners Compliance > HelpLine at > ??? http://www.partners.org/complianceline . If the e-mail was sent to you > in error > ??? but does not contain patient information, please contact the sender and > properly > ??? dispose of the e-mail. > > > ??? _______________________________________________ > ??? Histonet mailing list > ??? Histonet@lists.utsouthwestern.edu > > > ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 10 > Date: Thu, 2 Feb 2012 15:33:10 -0500 > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=UTF-8 > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that???s beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > >> >> Per SOP, we relabel, list date of receipt, test for quality and then apply >> a 12 month expiration date. We re-test after 12 months and continue to use, >> with 12 month dating, as long as the reagent meets quality standards set in >> the SOP. >> >> William DeSalvo, B.S., HTL(ASCP) >> >> >> >> > From: kstoll@mcw.edu >> > To: histonet@lists.utsouthwestern.edu >> > Date: Thu, 2 Feb 2012 12:56:58 -0600 >> > Subject: [Histonet] reagents without expiration dates >> > >> > Could anyone share a policy to deal with regents that do not have a >> manufacturer's expiration date? >> > CAP checklist ANP 21382 >> > >> > Thanks, >> > Kathryn Stoll, HT(ASCP) >> > Depatment of Pathology >> > Medical College of Wisconsin >> > 9200 W Wisconsin Ave >> > Milwaukee WI 53226 >> > 414.805.1525 >> > kstoll@mcw.edu >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ?_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 11 > Date: Thu, 2 Feb 2012 15:11:12 -0600 > From: "Horn, Hazel V" > Subject: [Histonet] histotech needed > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<25A4DE08332B19499904459F00AAACB719ACB9CBF2@EVS1.archildrens.org> > Content-Type: text/plain; charset="ISO-8859-1" > > Arkansas Children's Hospital is looking for a registered histotech. ?This is a dayshift, Monday-Friday position with rotating holidays on call. ?Our lab performs routine H&E's, special stains by hand and we have a Bond III immunostainer. ?We process approximately 6000 surgicals a year. ?ACH has excellent benefits. ?Little Rock is nice small city in which to live. ?We have many area attractions and if you like the outdoor life you will love Arkansas. ? ?The position is posted as an MLT, histology lab. ? Apply online at: > www.archildrens.org > > > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription > Arkansas Children's Hospital > 1 Children's Way ? ?Slot 820 > Little Rock, AR ? 72202 > > phone ? 501.364.4240 > fax ? ? ? ?501.364.3155 > > visit us on the web at: ? ?www.archildrens.org > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > > ------------------------------ > > Message: 12 > Date: Thu, 2 Feb 2012 13:14:53 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu, Emily Sours > ? ? ? ? > Message-ID: > ? ? ? ?<1328217293.25010.YahooMailClassic@web162101.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > Ha.Ha,Ha!!!!!!!!!!! > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > Try to beat that!. > Ren?? J. > > --- On Thu, 2/2/12, Emily Sours wrote: > > > From: Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > To: histonet@lists.utsouthwestern.edu > Date: Thursday, February 2, 2012, 3:33 PM > > > I've always wanted to have a contest to see who had the oldest reagents. > My lab once had something that was 20 years old. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that???s beautiful. > --Ron Swanson > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: > >> >> Per SOP, we relabel, list date of receipt, test for quality and then apply >> a 12 month expiration date. We re-test after 12 months and continue to use, >> with 12 month dating, as long as the reagent meets quality standards set in >> the SOP. >> >> William DeSalvo, B.S., HTL(ASCP) >> >> >> >> > From: kstoll@mcw.edu >> > To: histonet@lists.utsouthwestern.edu >> > Date: Thu, 2 Feb 2012 12:56:58 -0600 >> > Subject: [Histonet] reagents without expiration dates >> > >> > Could anyone share a policy to deal with regents that do not have a >> manufacturer's expiration date? >> > CAP checklist ANP 21382 >> > >> > Thanks, >> > Kathryn Stoll, HT(ASCP) >> > Depatment of Pathology >> > Medical College of Wisconsin >> > 9200 W Wisconsin Ave >> > Milwaukee WI 53226 >> > 414.805.1525 >> > kstoll@mcw.edu >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>?? _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 13 > Date: Thu, 2 Feb 2012 16:41:07 -0600 > From: "Joe Nocito" > Subject: Re: [Histonet] RE: Interviewing Histotechs... > To: "Kim Donadio" , "Jerry Ricks" > ? ? ? ? > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <8E6FB415E5AB409C90E5494BC877D04F@JoePC> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > ? ? ? ?reply-type=original > > yeah, I never did like that the "a monkey can do it" crap. A pathologist > told me that he could teach a monkey to gross. When the grossing tech messed > up a case, I was called in and got jumped on. He really didn't appreciate it > when I told him he better ?get that monkey trained quick. > > JTT > > ----- Original Message ----- > From: "Kim Donadio" > To: "Jerry Ricks" > Cc: > Sent: Tuesday, January 31, 2012 12:46 PM > Subject: Re: [Histonet] RE: Interviewing Histotechs... > > > Your comment about a monkey hits a nerve. There is a misconception I think > in our field that yes any monkey off the street can do our job. Well they > can't. It takes a good amount of knowledge to understand tissues, stains, > chemical reactions and yes you will need to have some ?amount of hand eye > coordination Skill . > > It's the monkey theory that has histotechs jumping up and down into these > quick almost no hands on programs so they can get a good paying job with not > much invested I'm afraid. Yes, I'm an expert at sticking my foot in my > mouth. But if we as a group don't recognize why we are even having this > debate about testing techs on the most basic of functions. ?Then I worry > about the future of our profession And even healthcare because by god if you > want monkeys , think monkeys , you will get monkeys! > > And darn it. I can't run and hide from this one can I lol > > Have a great week! > > Kim D > Sent from my iPhone > > On Jan 31, 2012, at 1:13 PM, Jerry Ricks wrote: > >> >> >> >> >> Hi Toysha >> >> I think I'm just coming at it from "research mode" not clinical. ?Hands on >> Histotechnology is a core part of our work, but just part, and it is >> focused on animal models of cardiovascular disease. ?Depending on whether >> the researcher is a postdoc or an undergrad they will have more or fewer >> general lab skills including histo skills. ?I haven't met anyone yet who >> did not need some training for embedding of brachiocephalic arteries of >> mice. >> >> I doubt I would do well in a clinical lab. ?I've become accustomed to docs >> saying "wow that's beautiful can you teach me how to do that?" ?I gather >> in the clinical field it's more like "a monkey can learn how to section." >> Maybe a good monkey could but I doubt it could work up an IHC with a new >> antibody. >> >> >> Jerry >> >> >>> From: TNMayer@mdanderson.org >>> To: histonet@lists.utsouthwestern.edu >>> Date: Tue, 31 Jan 2012 10:38:58 -0600 >>> Subject: [Histonet] RE: Interviewing Histotechs... >>> >>> Jerry, >>> I agree with you somewhat. ?I have met techs that misrepresented >>> themselves and said that they could cut or embed, and knew how to operate >>> the instruments, but could not produce quality work. ?You are right when >>> you said that it is different for clinical vs. research. I have almost >>> always worked clinical, and noticed that when working with research >>> techs, they had a difficult time adjusting to clinical with the time >>> frames and quality. >>> When training new hires, depending on the position I am hiring for, I >>> expect to train in the new workflow that they have learned, the new >>> instrument they use, not the basic skills. ?I only expect to do that with >>> a student. Fresh techs are expected to know how to get a section, not cut >>> the plastic on the block, embed skin, and set up the h&e stainer. ?I >>> should only have to go over and orient them on "our procedure" not teach >>> the skill. >>> I have worked various part-time jobs over the years and the first thing I >>> ask is 'how many microns do you cut at here'? While 3-4 is the standard, >>> some labs want everything at 3, or some at 4. ?I know how to cut, but >>> like you it takes about 2 weeks to get used to the new instrument. That's >>> fine, but I don't expect to have to teach the tech how to embed a skin or >>> cut a kidney biopsy. Not for an experienced tech, unless they have never >>> encountered it. ?That has to be made known during the interview. >>> Yes, a cutting test is good, I have seen registered techs not make it >>> past probation (90 days) because they could not cut. It would have saved >>> the company time and MONEY if a test could have been given. Asking if >>> they can cut a kidney biopsy, or embed a skin would be good as well. I >>> can't go back and get more epithelia or ask for another pass through the >>> kidney. >>> >>> >>> >>> Toysha N. Mayer, MBA, HT (ASCP) >>> Instructor, Education Coordinator >>> Program in Histotechnology >>> School of Health Professions >>> MD Anderson Cancer Center >>> (713) 563-3481 >>> tnmayer@mdanderson.org >>> >>> >>> >>> >>> >>> >>> Message: 5 >>> Date: Mon, 30 Jan 2012 11:13:11 -0800 >>> From: Jerry Ricks >>> Subject: [Histonet] Interviewing Histotechs... >>> To: >>> Message-ID: >>> Content-Type: text/plain; charset="iso-8859-1" >>> >>> >>> I gather it is different in clinical labs than in research labs. ?In >>> clinical labs there is an emphasis on quantity and speed. ?In research >>> the emphasis is on doing good experiments. ?Our "patients" are almost >>> always deceased or shortly about to be so there is no urgency of >>> diagnosis factor. ?For us, "diagnosis" means making precise measurements >>> else some scientists looking at an image and asking each other "what >>> the?" >>> >>> Anyway I always assume that the person I am hiring is incompetent at >>> histology and that they will need to be personally trained by me. >>> Doesn't matter how much experience they have. ?And over 23 years that has >>> turned out to be true. ?I've met exactly two people who didn't need much >>> training. ? ?One was a former senior clinical lab manager. ?The other was >>> a kid straight out of high school who happened to have a histology >>> experience from high school and a decent histo portfolio. ?Yes, Mercer >>> Island High School had a Histology program. >>> >>> No such thing as a tech who doesn't need to be trained and any tech >>> trained by me will be up and running in a week or two. ?Why bother making >>> them cut or stain anything during a darn interview. ?If they are smart >>> and cooperative they will work out. >>> >>> If I ever go to a new lab with a new microtome, new protocols, I am >>> pretty sure that I will be sort of incompetent for a week or two as well. >>> >>> Jerry Ricks >>> Research Scientist >>> University of Washington >>> Department of Pathology >>> >>> >>> >>> histonet@lists.utsouthwestern.edu >>>> Date: Sun, 29 Jan 2012 13:12:09 -0500 >>>> From: rsrichmond@gmail.com >>>> To: histonet@lists.utsouthwestern.edu >>>> Subject: [Histonet] Re: interview.... >>>> >>>> Ray Koelling asked me: >>>> >>>>>> If the Samurai Pathologist is out there reading still; any idea over >>>>>> your career, about how many glass slides have you viewed under a >>>>>> microscope since the first? Your replies are always top-notch, >>>>>> entertaining and informative. And hope with each new job you don't >>>>>> have to show someone you can pass a test of which slide shows normal >>>>>> liver and which slide shows cirrhotic liver in your interview.<< >>>> >>>> I really have no idea how many slides. In a normal year I sign out >>>> about 3,000 histology cases (remember I don't work full time) >>>> averaging maybe 3 slides per case. >>>> >>>> Generally I've gotten jobs, both private clients and agency clients, >>>> by recommendation. A number of years ago I was interviewed by a >>>> four-pathologist hospital group who handed me a tray of 20 slides with >>>> the necessary historical information, and was told that this was a set >>>> the group had collected, including very straightforward cases, cases >>>> with serious diagnostic pitfalls, and some cases they'd never been >>>> able to make a diagnosis on. They tried to make it a test of judgment >>>> rather than simple diagnostic skill. Told to take as much time as I >>>> needed. I guess I passed - by coincidence, the entire group chanced to >>>> break up very quickly, and an entirely different team took over. >>>> >>>> Bob Richmond >>>> Samurai Pathologist >>>> Knoxville TN >>>> >>> ************************************ >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Thu, 2 Feb 2012 22:50:10 +0000 > From: "Goins, Tresa" > Subject: RE: [Histonet] reagents without expiration dates > To: Rene J Buesa , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ?, Emily Sours > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="utf-8" > > OK, I'll give it a try Ren??. ?"Baker's Analyzed" Potassium Permanganate (5 lbs) with a "typed" label made with an actual "typewriter". ?Trying to verify age but I've wasted enough time already on fun stuff today. > > > > Tresa > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, February 02, 2012 2:15 PM > To: histonet@lists.utsouthwestern.edu; Emily Sours > Subject: Re: [Histonet] reagents without expiration dates > > > > Ha.Ha,Ha!!!!!!!!!!! > > I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). > > Try to beat that!. > > Ren?? J. > > > > --- On Thu, 2/2/12, Emily Sours > wrote: > > > > > > From: Emily Sours > > > Subject: Re: [Histonet] reagents without expiration dates > > To: histonet@lists.utsouthwestern.edu > > Date: Thursday, February 2, 2012, 3:33 PM > > > > > > I've always wanted to have a contest to see who had the oldest reagents. > > My lab once had something that was 20 years old. > > > > Emily > > > > The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that???s beautiful. > > --Ron Swanson > > > > > > > > On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO >wrote: > > > >> > >> Per SOP, we relabel, list date of receipt, test for quality and then > >> apply a 12 month expiration date. We re-test after 12 months and > >> continue to use, with 12 month dating, as long as the reagent meets > >> quality standards set in the SOP. > >> > >> William DeSalvo, B.S., HTL(ASCP) > >> > >> > >> > >> > From: kstoll@mcw.edu > >> > To: histonet@lists.utsouthwestern.edu > >> > Date: Thu, 2 Feb 2012 12:56:58 -0600 > >> > Subject: [Histonet] reagents without expiration dates > >> > > >> > Could anyone share a policy to deal with regents that do not have a > >> manufacturer's expiration date? > >> > CAP checklist ANP 21382 > >> > > >> > Thanks, > >> > Kathryn Stoll, HT(ASCP) > >> > Depatment of Pathology > >> > Medical College of Wisconsin > >> > 9200 W Wisconsin Ave > >> > Milwaukee WI 53226 > >> > 414.805.1525 > >> > kstoll@mcw.edu > >> > > >> > _______________________________________________ > >> > Histonet mailing list > >> > Histonet@lists.utsouthwestern.edu > >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> ?_______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 15 > Date: Thu, 2 Feb 2012 16:02:34 -0700 > From: WILLIAM DESALVO > Subject: RE: [Histonet] reagents without expiration dates > To: , histonet , > ? ? ? ? > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich and was visiting Charles Churukian's lab. He had a full wall of his lab with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had every lab chemical and reagent I knew and was very proud that he was a great Sigma customer. The labels were none that I had seen in 10 yrs working at Sigma, so I took a few containers down and read the manufacturing code. The oldest was sodium bisulfate, gallon container, manufactured in 1946. Most were 20-30 yrs old then and in large quantities. I have always thought to myself, with many more customers like Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still teaching the heavens everything they need to know about Histology and Staining. I don't know if that beats your stuff, but I bet the chemicals Chuck left behind are still in use. > > William DeSalvo, B.S., HTL(ASCP) > > > >> Date: Thu, 2 Feb 2012 13:14:53 -0800 >> From: rjbuesa@yahoo.com >> To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com >> Subject: Re: [Histonet] reagents without expiration dates >> CC: >> >> Ha.Ha,Ha!!!!!!!!!!! >> I used to prepare staining solutions with some "Merck-Darmstad" anilines manufactured just after the "Great War", i.e. the FIRST World War (about 1925 before the World Great Depression). >> Try to beat that!. >> Ren? J. >> >> --- On Thu, 2/2/12, Emily Sours wrote: >> >> >> From: Emily Sours >> Subject: Re: [Histonet] reagents without expiration dates >> To: histonet@lists.utsouthwestern.edu >> Date: Thursday, February 2, 2012, 3:33 PM >> >> >> I've always wanted to have a contest to see who had the oldest reagents. >> My lab once had something that was 20 years old. >> >> Emily >> >> The whole point of this country is if you want to eat garbage, balloon up >> to 600 pounds and die of a heart attack at 43, you can! You are free to do >> so. To me, that?s beautiful. >> --Ron Swanson >> >> >> >> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO wrote: >> >> > >> > Per SOP, we relabel, list date of receipt, test for quality and then apply >> > a 12 month expiration date. We re-test after 12 months and continue to use, >> > with 12 month dating, as long as the reagent meets quality standards set in >> > the SOP. >> > >> > William DeSalvo, B.S., HTL(ASCP) >> > >> > >> > >> > > From: kstoll@mcw.edu >> > > To: histonet@lists.utsouthwestern.edu >> > > Date: Thu, 2 Feb 2012 12:56:58 -0600 >> > > Subject: [Histonet] reagents without expiration dates >> > > >> > > Could anyone share a policy to deal with regents that do not have a >> > manufacturer's expiration date? >> > > CAP checklist ANP 21382 >> > > >> > > Thanks, >> > > Kathryn Stoll, HT(ASCP) >> > > Depatment of Pathology >> > > Medical College of Wisconsin >> > > 9200 W Wisconsin Ave >> > > Milwaukee WI 53226 >> > > 414.805.1525 >> > > kstoll@mcw.edu >> > > >> > > _______________________________________________ >> > > Histonet mailing list >> > > Histonet@lists.utsouthwestern.edu >> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ?_______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 16 > Date: Thu, 2 Feb 2012 15:07:20 -0800 > From: Jennifer MacDonald > Subject: RE: [Histonet] reagents without expiration dates > To: WILLIAM DESALVO > Cc: histonet , > ? ? ? ?histonet-bounces@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="UTF-8" > > I had a student that was working in a lab with old dry powders. ?One that > I recall had a "date opened" of 1942. > > > > > WILLIAM DESALVO > Sent by: histonet-bounces@lists.utsouthwestern.edu > 02/02/2012 03:04 PM > > To > , histonet , > > cc > > Subject > RE: [Histonet] reagents without expiration dates > > > > > > > > Many, many, many years ago back in the 80's, I worked for Sigma-Aldrich > and was visiting Charles Churukian's lab. He had a full wall of his lab > with shelves, floor to ceiling, of dried and liquid Sigma reagents. He had > every lab chemical and reagent I knew and was very proud that he was a > great Sigma customer. The labels were none that I had seen in 10 yrs > working at Sigma, so I took a few containers down and read the > manufacturing code. The oldest was sodium bisulfate, gallon container, > manufactured in 1946. Most were 20-30 yrs old then and in large > quantities. I have always thought to myself, with many more customers like > Chuck, Sigma could go out of business. I miss Chuck, but I bet he is still > teaching the heavens everything they need to know about Histology and > Staining. I don't know if that beats your stuff, but I bet the chemicals > Chuck left behind are still in use. > > William DeSalvo, B.S., HTL(ASCP) > > > >> Date: Thu, 2 Feb 2012 13:14:53 -0800 >> From: rjbuesa@yahoo.com >> To: histonet@lists.utsouthwestern.edu; talulahgosh@gmail.com >> Subject: Re: [Histonet] reagents without expiration dates >> CC: >> >> Ha.Ha,Ha!!!!!!!!!!! >> I used to prepare staining solutions with some "Merck-Darmstad" anilines > manufactured just after the "Great War", i.e. the FIRST World War (about > 1925 before the World Great Depression). >> Try to beat that!. >> Ren?? J. >> >> --- On Thu, 2/2/12, Emily Sours wrote: >> >> >> From: Emily Sours >> Subject: Re: [Histonet] reagents without expiration dates >> To: histonet@lists.utsouthwestern.edu >> Date: Thursday, February 2, 2012, 3:33 PM >> >> >> I've always wanted to have a contest to see who had the oldest reagents. >> My lab once had something that was 20 years old. >> >> Emily >> >> The whole point of this country is if you want to eat garbage, balloon > up >> to 600 pounds and die of a heart attack at 43, you can! You are free to > do >> so. To me, that???s beautiful. >> --Ron Swanson >> >> >> >> On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO > wrote: >> >> > >> > Per SOP, we relabel, list date of receipt, test for quality and then > apply >> > a 12 month expiration date. We re-test after 12 months and continue to > use, >> > with 12 month dating, as long as the reagent meets quality standards > set in >> > the SOP. >> > >> > William DeSalvo, B.S., HTL(ASCP) >> > >> > >> > >> > > From: kstoll@mcw.edu >> > > To: histonet@lists.utsouthwestern.edu >> > > Date: Thu, 2 Feb 2012 12:56:58 -0600 >> > > Subject: [Histonet] reagents without expiration dates >> > > >> > > Could anyone share a policy to deal with regents that do not have a >> > manufacturer's expiration date? >> > > CAP checklist ANP 21382 >> > > >> > > Thanks, >> > > Kathryn Stoll, HT(ASCP) >> > > Depatment of Pathology >> > > Medical College of Wisconsin >> > > 9200 W Wisconsin Ave >> > > Milwaukee WI 53226 >> > > 414.805.1525 >> > > kstoll@mcw.edu >> > > >> > > _______________________________________________ >> > > Histonet mailing list >> > > Histonet@lists.utsouthwestern.edu >> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ?_______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ? ?_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 17 > Date: Fri, 03 Feb 2012 09:14:24 +1000 > From: "Tony Reilly" > Subject: Re: AW: [Histonet] C4d IF on FFPE kidney > To: , "'Richard Cartun'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4F2BA56F.411C.0039.0@health.qld.gov.au> > Content-Type: text/plain; charset="UTF-8" > > Hi Gudren > > We have been doing both IF and IHC for C4d for quite a while. ?While we > have found that the IF staining is superior to the IHC we are not > unhappy with the IHC results. ?Our IHC results have improved since > getting a Ventana Ultra which has allowed better control of the > staining. > > regards > Tony > > > > > > Tony Reilly ?B.App.Sc. , M.Sc. > Chief Scientist, Anatomical Pathology > Pathology Queensland-PA Laboratory > _________________________________________________ > Clinical and Statewide Services Division| QueenslandHealth > > Level 1, Building 15,Princess Alexandra Hospital > Ipswich Road,WOOLLOONGABBA ?Qld4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > Fax: 07 3176 2930 > Email: tony_reilly@health.qld.gov.au > Web: ?www.health.qld.gov.au/qhcss/ > > > > >>>> "Gudrun Lang" 2/3/2012 12:31 am >>> > We have been performing C4d IHC on FFPE kidney for a couple of years. > We > receive only fixed samples. > Yesterday my doctors came with this idea of IF - I still have to ask > for the > special reasons. But I have the suspicion, that they are not aware of > the > fact, that IF on frozen unfixed tissue is the usual way found in > literature. > > There are some articles that deal with comparison of IF(frozen) and > IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows > additional staining in glomeruli. > > Perhaps someone told them, that IF is the golden standard and > recommended. > > Gudrun > > -----Urspr??ngliche Nachricht----- > Von: Richard Cartun [mailto:Rcartun@harthosp.org] > Gesendet: Mittwoch, 01. Februar 2012 21:21 > An: gu.lang@gmx.at > Betreff: Re: [Histonet] C4d IF on FFPE kidney > > Hi Gudrun: > > Is there reason why you want to use IF and not immunoperoxidase? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > >>>> "Gudrun Lang" 2/1/2012 3:17 PM >>> > Hi! > > Can someone provide me a immunofluorescence protocol for C4d on > formalin > fixed human paraffin sections? > > > > thanks in advance > > > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. ?The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. ?You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. > Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. > ********************************************************************************** > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 3 > *************************************** From madeleinehuey <@t> gmail.com Sun Feb 5 00:27:06 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sun Feb 5 00:27:12 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 2 In-Reply-To: <4f2acf4b.234cec0a.15e2.78f9SMTPIN_ADDED@mx.google.com> References: <4f2acf4b.234cec0a.15e2.78f9SMTPIN_ADDED@mx.google.com> Message-ID: Gudrun, I don't understand why you say "IF on frozen unfixed tissue is the usual way found in literature". IF can be done on any type of tissues. By any chance your doctors ask for multiple staining with the IF? Maybe he's looking for co-localization, that's the best way. Here's an example of IF; 1) Dep. & hydration 2) HIER; AR with Diva (Biocare) for 3'-5' with pressure cooker, rinse with washing buffer immediately (no need cooling for 20 min @ RT) 3) 1st Ab; incubate Rabbit Monoclonal anti - C4d 1:50 (Spring Bioscience) for 60 min in dark "you can use your existing 1st ab, but do different titration to optimized" 4) 2nd Ab; incubate Alexa 594/___ anti-Rabbit (1:100) for 60 min in dark "whatever 2nd Abs & titre you have in your lab" 5) Counterstain with DAPI (whatever you have in the lab) for 5 min in dark 6) Coverslip with your usual IF mounting solution (ie. Prolong gold, etc) Madeleine Huey, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Thu, Feb 2, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Re: Looking for Part-Time Volunteer Work (Paula) > ? 2. CLIA Inspection (Fawn Bomar) > ? 3. Job Listing Houston,Tx (Stella Mireles) > ? 4. On call Position in Chicago (Lester Raff MD) > ? 5. RE: RAC Medicare Audits - PAs (Richard Cartun) > ? 6. C4d IF on FFPE kidney (Gudrun Lang) > ? 7. Re: Bleaching in the histo lab (Lee & Peggy Wenk) > ? 8. Re: RAC Medicare Audits - PAs (Kim Donadio) > ? 9. Re: Bleaching in the histo lab (Kim Donadio) > ?10. RE: IHCRG where are you? ?(JMyers1@aol.com) > ?11. AW: [Histonet] C4d IF on FFPE kidney (Gudrun Lang) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 1 Feb 2012 10:03:20 -0800 (PST) > From: Paula > Subject: [Histonet] Re: Looking for Part-Time Volunteer Work > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ?<1328119400.2279.YahooMailClassic@web30302.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I am in Raleigh, NC. Looking for part-time volunteer work to build skills up. > > Paula > > > > ------------------------------ > > Message: 2 > Date: Wed, 1 Feb 2012 18:46:04 +0000 > From: Fawn Bomar > Subject: [Histonet] CLIA Inspection > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<0111BC10D77DC54EAB99B2DDA3BCE4B916B41A@EXCH-2K10.hrhs.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi everyone! > > > > I was wondering if anyone out there that has knowledge of CLIA regs and inspections would be willing to contact me directly to help me answer a few questions. > > > > My contact info is Fawn.Bomar@halifaxregional.com or call if it is easier at 434-517-3033. > > > > Thank you in advance, > > Fawn > > > ------------------------------ > > Message: 3 > Date: Wed, 1 Feb 2012 13:24:48 -0600 > From: Stella Mireles > Subject: [Histonet] Job Listing Houston,Tx > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=windows-1252 > > ------------------------------ > ?*ANNOUNCEMENT NUMBER: 14905- T* > * * > *JOB TITLE: ? ? ? ? ? ? ? Histology Technician II * > * * > *DEPARTMENT: ? ? ? ?Institute of Forensic Sciences* > * * > *HOURS: ? ? ? ? ? ? ? ? ? ? 7:30 a.m. ? 4:40 p.m. / Flexible* > * ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Monday - Friday* > * * > *SALARY: ? ? ? ? ? ? ? ? ? ?Commensurate With Experience* > * ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Based On 26 Pay Periods* > * * > *EDUCATION: ?*Completion of an Associate?s degree and completion of > histology school with histo-technician certification (ASCP) American > Society of Clinical Pathologist *at the time of employment or within one > year of employment. ?* > * * > *EXPERIENCE: ?*One year of experience in a histology laboratory in which > responsibilities included production of stained slides and preservation of > tissue samples in both paraffin and formalin is *required*.** > * * > *JOB SKILLS: ?*The successful applicant must have expertise in the use of > microtomes, manual staining procedures, manual slide coverslipping, > automated slide stainers, automated slide coverslipper, tissue processors > and tissue embedding. ?Must be capable of understanding and adhering to > strict protocols for the handling, trimming and archival of tissue samples > and blocks; knowledge of histology laboratory safety rules and procedures *is > essential*. ?Good interpersonal skills *are a must*. > > *JOB DESCRIPTION: ?*Prepares stained slides of autopsy tissues in a > careful, controlled environment. ?Prepares paraffin blocks of tissue for > long-term storage and labels the samples in accordance with histology > laboratory protocols; assists with archival of paraffin-embedded and > non-paraffin-embedded formalin fixed tissues. Provides all tissue slides to > the assigned Assistant Medical Examiner, Deputy Chief Medical Examiner or > Chief Medical Examiner on a timely basis; assists with data entry into a > computerized database to track laboratory efficiency as required. ?Other > job assignments as assigned by the Deputy Chief Medical Examiner. ?*Position > requires a high level of confidentiality, responsibility and dependability.* > > *PHYSICAL REQUIREMENTS:** ?*Must be able to sit and stand for prolonged > periods of time; able to lift up to 40lbs; stooping and bending may be > required. > * * > *EMPLOYMENT IS CONTINGENT UPON PASSING A CRIMINAL BACKGROUND CHECK.* > * * > * * > *HARRIS COUNTY HAS AN EMPLOYMENT AT WILL POLICY.* > *CLOSING DATE: ? ? ?Open Until Filled* > *APPLY AT: ? ? ? ? ? ? ? ?1310 PRAIRIE - SUITE 170* > * * > * * > *UPON RECEIVING A CONDITIONAL OFFER OF EMPLOYMENT, ALL APPLICANTS ARE > SCREENED FOR THE PRESENCE OF ILLEGAL DRUGS.*** > > > ------------------------------ > > Message: 4 > Date: Wed, 1 Feb 2012 13:37:55 -0600 > From: "Lester Raff MD" > Subject: [Histonet] On call Position in Chicago > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Our private outpatient specialty lab has an opening for an on-call > part-time second shift and weekend histologist. We are located in the > western Chicago suburbs about a mile east of Oak Brook Shopping Center. > If interested, please contact Andrea O'Brien at 708-486-0076 > . Experienced histologists only, please. > > > > > > > > Lester J. Raff, MD > > Medical Director > > UroPartners Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel 708.486.0076 > > Fax 708.492.0203 > > > > > > ------------------------------ > > Message: 5 > Date: Wed, 01 Feb 2012 14:45:06 -0500 > From: "Richard Cartun" > Subject: RE: [Histonet] RAC Medicare Audits - PAs > To: "Histonet" , ? ? "Loralee A > ? ? ? ?McMahon" > Message-ID: <4F294FF2.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I don't know. ?I was hoping that there is someone in the Histonet community that is familiar with this. > > Richard > >>>> "McMahon, Loralee A" 2/1/2012 8:56 AM >>> > I haven't heard that one. ? Do they mean present physically or present as in their office reading slides, but a phone call or page away? > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] > Sent: Tuesday, January 31, 2012 7:19 PM > To: Histonet > Subject: [Histonet] RAC Medicare Audits - PAs > > Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Wed, 1 Feb 2012 21:17:14 +0100 > From: "Gudrun Lang" > Subject: [Histonet] C4d IF on FFPE kidney > To: > Message-ID: <138B99031E744674A4C020CB44691D27@dielangs.at> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Hi! > > Can someone provide me a immunofluorescence protocol for C4d on formalin > fixed human paraffin sections? > > > > thanks in advance > > > > Gudrun Lang > > > > ------------------------------ > > Message: 7 > Date: Wed, 1 Feb 2012 18:03:21 -0500 > From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] Bleaching in the histo lab > To: "angela smith" , > ? ? ? ? > Message-ID: > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > ? ? ? ?reply-type=original > > Several questions and comments, in no particular order: > > 1. What percent of bleach? > - 10% is all that is needed for biohazards. If you are concerned about the > smell, it might be too high a percent. > > 2. How good is your ventilation? How long do you continue to smell the > chlorine? > - If you continue to smell it hours later, or even the next day, have your > safety officer and maintenance people check out the ventilation. > > 3. After wiping down with 10% bleach, are you wiping down the counter with > water? > - Need to clean off the corrosive bleach off the surfaces. That would also > help with the smell. But takes more time. > > 3. What locations in the lab are you cleaning with dilute bleach? > - The only areas that need to be cleaned with a disinfectant are those areas > that have fresh or not completely fixed tissue, so around the grossing > stations and the cryostat. Maybe where specimens are received into the lab. > - The areas where you process tissue, embed, microtome, do staining, file > slides and blocks should not need to be disinfected with bleach. The tissue > has been fixed in formalin, and gone through alcohol, xylene (or > substitute), and placed in 60 degree C (140 degree F) paraffin. That should > kill almost all microorganisms. Therefore, should not need to clean up with > anything beyond soap and water. If you have a very underprocessed tissue > block, and it's oozing and weeping all over the counter and microtome, you > may want to disinfect the area. (If it's a CJD case, you are going to need > strong solutions than 10% bleach, but that's a whole new conversation.) > - So talk with your safety officer, about how there are no biohazards in the > other parts of the lab. They may be thinking more of the clinical pathology > labs, with blood tubes and petri dishes, needing to be disinfected with > bleach every day/shift. > > 4. Chemical incompatibility: > Bleach is incompatible with ammonia (makes chlorine gas - deadly) > Bleach is incompatible with acids > Bleach is an oxidizer, and formaldehyde is supposed to be kept away from > oxidizers. > So, yes, I would be a little worried about chemical interaction. However, > wiping down the area first with water, to remove other chemicals, before the > bleach, would take care of this problems. > > 5. What does Epidemiology suggest for disinfectant? > Our epidemiology is suggesting other cleaning solutions for disinfecting, > rather than bleach, in many cases. > - not as corrosive > - less obnoxious fumes > - more "green" > - better disinfectant and faster, than bleach > > Peggy Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > (Comments reflect my opinions, not that of my hospital) > > -----Original Message----- > From: angela smith > Sent: Wednesday, February 01, 2012 8:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bleaching in the histo lab > > I have been told by our safety officer that it is standard practice too > clean the lab at the end of the day with diluted bleach. I have noticed a > chemical reaction (smell) when cleaning the main area of the lab. I have > concerns that this is not a good practice due to chemical reactions as we > use so many chemicals in histology. What do other people do? ?Also I believe > it is unsafe to use bleach with anything formalin related. > Please let me know if you have a "standard" practice or mandated cleaning > from your facility. > Angela > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 8 > Date: Wed, 1 Feb 2012 19:26:45 -0500 > From: Kim Donadio > Subject: Re: [Histonet] RAC Medicare Audits - PAs > To: Richard Cartun > Cc: Histonet , ? ? ? Loralee A McMahon > ? ? ? ? > Message-ID: <65FAEDF0-7CFC-4188-8061-2382A42A7C00@yahoo.com> > Content-Type: text/plain; ? ? ? charset=us-ascii > > Sorry. ?But I thought RAC was a group of individuals who go over your billing looking for over payments to Medicare ? They typically get a % of what they find so it motivates them. If u have this group of auditors in your area and they Are saying this then I would just ask them to show you the rule they must be referring to a CLIA guideline somewhere. > Best of luck > Kim D > > Sent from my iPhone > > On Feb 1, 2012, at 2:45 PM, "Richard Cartun" wrote: > >> I don't know. ?I was hoping that there is someone in the Histonet community that is familiar with this. >> >> Richard >> >>>>> "McMahon, Loralee A" 2/1/2012 8:56 AM >>> >> I haven't heard that one. ? Do they mean present physically or present as in their office reading slides, but a phone call or page away? >> >> Loralee McMahon, HTL (ASCP) >> Immunohistochemistry Supervisor >> Strong Memorial Hospital >> Department of Surgical Pathology >> (585) 275-7210 >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] >> Sent: Tuesday, January 31, 2012 7:19 PM >> To: Histonet >> Subject: [Histonet] RAC Medicare Audits - PAs >> >> Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & Immunopathology >> Director, Biospecimen Collection Programs >> Assistant Director, Anatomic Pathology >> Hartford Hospital >> 80 Seymour Street >> Hartford, CT ?06102 >> (860) 545-1596 Office >> (860) 545-2204 Fax >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 1 Feb 2012 19:51:45 -0500 > From: Kim Donadio > Subject: Re: [Histonet] Bleaching in the histo lab > To: Lee & Peggy Wenk > Cc: "" > ? ? ? ? > Message-ID: > Content-Type: text/plain; ? ? ? charset=us-ascii > > Maybe those scented bleach wiped would work. I love those things and they smell pretty good. ?Just a thought. I like the greencan fresh scent > products/clorox-disinfecting-wipes/ > KimD > Sent from my iPhone > > On Feb 1, 2012, at 6:03 PM, "Lee & Peggy Wenk" wrote: > >> >> - Need to clean off the corrosive bleach off the surfaces. That would also > > > > ------------------------------ > > Message: 10 > Date: Thu, 2 Feb 2012 06:38:33 -0500 (EST) > From: JMyers1@aol.com > Subject: RE: [Histonet] IHCRG where are you? > To: histonet@lists.utsouthwestern.edu > Cc: broyce@nsh.org, cdiamond@nsh.org, Carol.Freeman@utoledo.edu > Message-ID: <9a4e.313de094.3c5bcfb9@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Carol: > I'm happy to report that the IHC Resource Group remains alive and ?well. > For reasons unkown, the group's website is currently not working ?properly, > and through this message I've notified members of the NSH's staff ?of the > problem. ?We are actively working on a number of projects, most ?notably the > annual IHC Forum event, and once our site is working again we hope ?to post new > information. ?Stay tuned for details... > Sincerely, > Joe Myers, M.S, CT(ASCP)QIHC > Chair, IHC Resource Group > ------------------------------ > > Message: 9 > Date: Wed, 1 Feb 2012 ?10:33:08 -0500 > From: "Freeman, Carol" ? > Subject: [Histonet] IHCRG where are ?you? > To: _histonet@lists.utsouthwestern.edu_ > (mailto:histonet@lists.utsouthwestern.edu) > > Good ?Morning, > > Just curious if any one out there knows what happened to the ?IHCRG (IHC > review group?) It was an organization advertised in the NSH ?material I > brought home from last September but the website doesn't exist and ?the > email address listed was undeliverable...Just curious It sounded like ?an > interest idea, just wondered what happened.. Thank you for any ?response > :) > > > ------------------------------ > > Message: 11 > Date: Thu, 2 Feb 2012 15:31:04 +0100 > From: "Gudrun Lang" > Subject: AW: [Histonet] C4d IF on FFPE kidney > To: "'Richard Cartun'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <206DA8F070EC4221B7C45F02B3A99C1C@dielangs.at> > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > We have been performing C4d IHC on FFPE kidney for a couple of years. We > receive only fixed samples. > Yesterday my doctors came with this idea of IF - I still have to ask for the > special reasons. But I have the suspicion, that they are not aware of the > fact, that IF on frozen unfixed tissue is the usual way found in literature. > > There are some articles that deal with comparison of IF(frozen) and > IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows > additional staining in glomeruli. > > Perhaps someone told them, that IF is the golden standard and recommended. > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: Richard Cartun [mailto:Rcartun@harthosp.org] > Gesendet: Mittwoch, 01. Februar 2012 21:21 > An: gu.lang@gmx.at > Betreff: Re: [Histonet] C4d IF on FFPE kidney > > Hi Gudrun: > > Is there reason why you want to use IF and not immunoperoxidase? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > >>>> "Gudrun Lang" 2/1/2012 3:17 PM >>> > Hi! > > Can someone provide me a immunofluorescence protocol for C4d on formalin > fixed human paraffin sections? > > > > thanks in advance > > > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 2 > *************************************** From amosbrooks <@t> gmail.com Sun Feb 5 08:47:12 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Feb 5 08:47:16 2012 Subject: [Histonet] picric acid Message-ID: Hi, The largest disaster I know of related to picric acid (among other things) is one that every one working with it should keep in mind. The Halifax explosion basically leveled the city of Halifax, Nova Scotia, Canada in 1917. While they were carrying much more than the 500 ml to 1 gallon that we might use it is worth noting the magnitude of what this caused. There is a really good Wikipedia article on it here: http://en.wikipedia.org/wiki/Halifax_Explosion This was a terrible disaster and it underscores why we need to be really conscious of the chemicals we work with, and even the ones we haven't used in years. I would also like to play Devil's advocate here though. Yes there are inherent hazards with many chemicals we work with. But, we also need to be able use these chemicals in a safe manner. If used safely, these chemicals can be used for stains that cannot really be replicated with substitutes. Picro-sirius red is a good example of this. The solution to hazardous chemicals is not getting rid of them and burying your head in the sand. It is education and understanding of the hazards and using them properly. Amos Brooks From koellingr <@t> comcast.net Sun Feb 5 13:05:54 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Feb 5 13:06:12 2012 Subject: [Histonet] picric acid In-Reply-To: Message-ID: <17540967.468210.1328468754457.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> I whole-heartedly agree with and applaud Amos Brooks playing Devil's advocate. I certainly would never discount the degree of danger with what is a high explosive and would take all due caution using disposal people who know what they are doing. But there is a use for the substance and one needs to separate mystery and uncertainty and incomplete facts and anecdote from actual fact. It is my understanding that while pure, crystalline picric acid might be unstable and shock sensitive (and a danger to a degree), it is the metal or salt picrates that are way, way more dangerous. Thus warnings in chemistry for picric acid; don't use metal spatula's. No metal cans or metal caps. Don't drop on concrete (silica and other things). Don't dispose down drain (lead or other metals). When viewing the chemistry guy in college who "blows up" a minute amount of picric acid on an asbestos pad over a Bunsen burner it is NOT pure picric acid but PA plus Pb (Lead) salt. While artillery shells might have been filled with picric acid they were relatively stable but they became way more unstable if the picric acid reacts with the metal casing or fuse casing (the infamous Halifax Explosion???). Thus the following to my utter dismay. You hear or watch on youtube a "bomb disposal unit"?? blowing up a glass jar of dangerous picric acid they remove from school. In the field right next to a soccer goal post. As a former soccer goalie who used to dive all over the ground my question is; is anyone picking up the thousands of shards of glass from the soccer field? And what about the dispersed picric acid since no chemical reaction is 100% efficient and there must be dangerous picric acid (residue) all over the place. It is not well known but prior to the Trinity blast there was a lot of study of encasing the bomb in an enormous containment vessel called Jumbo. If the lens shaped, multi-firing pin, high explosive nest surrounding the sub-critcal fissionable mass had mis-fired by even a milli-second, the core would have blown to one side instead of attaining criticality by being compressed. As there was very little fissionable grade material on the face of the earth at that point, they wanted to retrieve it "clean it from the environments and re-use it" by containing it. What if they had used Jumbo (it still sits, unused hundreds of yards away from detonation point), while most of it would have vaporized in the successful test, might there not be shards of highly radioactive bits of metal scattered and raining down for hundreds of square miles including on sports fields near towns. Where soccer goalies play? Ray Ray Koelling Seattle, Washington ----- Original Message ----- From: "Amos Brooks" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 5, 2012 6:47:12 AM Subject: [Histonet] picric acid Hi, The largest disaster I know of related to picric acid (among other things) is one that every one working with it should keep in mind. The Halifax explosion basically leveled the city of Halifax, Nova Scotia, Canada in 1917. While they were carrying much more than the 500 ml to 1 gallon that we might use it is worth noting the magnitude of what this caused. There is a really good Wikipedia article on it here: http://en.wikipedia.org/wiki/Halifax_Explosion This was a terrible disaster and it underscores why we need to be really conscious of the chemicals we work with, and even the ones we haven't used in years. I would also like to play Devil's advocate here though. Yes there are inherent hazards with many chemicals we work with. But, we also need to be able use these chemicals in a safe manner. If used safely, these chemicals can be used for stains that cannot really be replicated with substitutes. Picro-sirius red is a good example of this. The solution to hazardous chemicals is not getting rid of them and burying your head in the sand. It is education and understanding of the hazards and using them properly. Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sun Feb 5 16:40:03 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Feb 5 16:40:07 2012 Subject: [Histonet] Re: picric acid Message-ID: Picric acid (trinitrophenol) is structurally related to trinitrotoluene (TNT) but is much easier to synthesize, so it was used as a military explosive earlier than TNT. Unlike TNT, which must be detonated with a primer, picric acid can be detonated directly or even explode spontaneously. It's the dry chemical that's explosive. Bottled with 10% water, it's actually quite safe - it's when the water evaporates that it gets dangerous. In Europe World War 1 steel hand grenades, full of either picric acid or TNT corroding into picric acid, are considered the most dangerous of 20th century munitions that are still occasionally found on old battlegrounds. I was interested to read here on Histonet three accounts of actual explosions of laboratory picric acid - I was never sure before that these weren't urban legends - as well as of the truly horrible Halifax disaster. I don't think histology labs should keep solid picric acid at all. You can either buy Bouin's fixative ready made or mix your own, using the saturated solution of picric acid in water that's still available for labs doing Jaffe reaction determinations of creatinine. Bob Richmond From Vickroy.Jim <@t> mhsil.com Mon Feb 6 08:18:16 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Feb 6 08:18:25 2012 Subject: [Histonet] Billing for stereotactic breast biopsies in core containers Message-ID: <24A4826E8EF0964D86BC5317306F58A55FDC2EC190@mmc-mail.ad.mhsil.com> How are people billing these? Currently we are billing as one 88305 however I am wondering if others are billing for each well separately. (such as 6 o'clock, 9'oclock, 12'oclock, 3 o'clock, and center well). James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From mw <@t> personifysearch.com Mon Feb 6 08:26:45 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Feb 6 08:26:55 2012 Subject: [Histonet] New Histology Opportunities In-Reply-To: <1a817c77dcb05a27c3c4341e4a80c053@mail.gmail.com> References: <1a817c77dcb05a27c3c4341e4a80c053@mail.gmail.com> Message-ID: Good Morning Histonet, Personify has had 2 new permanent Histology opportunities become available in the Northern IL area. Please contact me directly at mw@personifysearch to learn more. *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacture and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunities:* (1) QA Histologist: The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. (2) Histologist: The company currently has an opening for a Histologist to be based in Richmond IL. Salary: Based on Experience Other: Full benefits - 401k program/matching *Education and Experience Required:* - Experience with tissue grossing, tissue processing and embedding - Experience with sectioning paraffin embedded tissue as well as frozen tissue - Experience performing routine stains (H and E) as well as special stains - Experience with formulation and production of routine laboratory reagents and solutions - Experience performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Proficiency in basic computer skills and with software applications such as Microsoft Office Have a great day! Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From Vickroy.Jim <@t> mhsil.com Mon Feb 6 09:12:16 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Feb 6 09:12:24 2012 Subject: [Histonet] Coding for Rectum segment for prolapse Message-ID: <24A4826E8EF0964D86BC5317306F58A55FDC2EC202@mmc-mail.ad.mhsil.com> In reviewing the CPT codes I do not see a lower code for a portion of rectum taken out for prolapse. Does anybody have an idea how they are charging or coding these specimens? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From JWeems <@t> sjha.org Mon Feb 6 09:34:13 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Feb 6 09:34:27 2012 Subject: [Histonet] RE: Coding for Rectum segment for prolapse In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55FDC2EC202@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55FDC2EC202@mmc-mail.ad.mhsil.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846E424AA@CHEXCMS10.one.ads.che.org> I would code as an 07 - Colon, Segmental Resection other that tumor Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, February 06, 2012 10:12 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coding for Rectum segment for prolapse In reviewing the CPT codes I do not see a lower code for a portion of rectum taken out for prolapse. Does anybody have an idea how they are charging or coding these specimens? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From doakes <@t> olympicmedical.org Mon Feb 6 12:17:25 2012 From: doakes <@t> olympicmedical.org (Dawn Oakes) Date: Mon Feb 6 12:17:32 2012 Subject: [Histonet] FATTY TISSUSE Message-ID: We would like to know what rapid fix solution is being used prior to putting fatty tissues on the tissue processor with alcoholic formalin.? We do not have the availability of a separate program. Thanks , Dawn Oakes HT ASCP Olympic Medical Center 939 Caroline Street Port Angeles, WA 98362 360-417-7467 ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From rjbuesa <@t> yahoo.com Mon Feb 6 14:04:20 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 6 14:04:28 2012 Subject: [Histonet] FATTY TISSUSE In-Reply-To: Message-ID: <1328558660.81495.YahooMailClassic@web162104.mail.bf1.yahoo.com> There is no "rapid solution" to fixing fatty tissue. By its own nature of being "fatty"?it hampers the formalin penetration and require more time than other tissues. If you use alcoholic-formalin, you are fixing it with ethanol and with formalin INDEPENDENTLY because each will penetrate the tissue at a different rate. If you cut the fixation time you will end with an alcoholic fixation, reinforced with the dehydrating alcohols, but it will be lacking the formalin fixation. Take your time and you will avoid improper infiltration consequence of an improper fixation. Ren? J. --- On Mon, 2/6/12, Dawn Oakes wrote: From: Dawn Oakes Subject: [Histonet] FATTY TISSUSE To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 6, 2012, 1:17 PM We would like to know what rapid fix solution is being used prior to putting fatty tissues on the? tissue processor with alcoholic formalin.? We do not have the availability of a separate program. Thanks , Dawn Oakes HT ASCP Olympic Medical Center 939 Caroline Street Port Angeles, WA 98362 360-417-7467 ------------------------------------------------------------------------- Confidentiality Notice:? This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.? Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.? If you are not the intended recipient, you have received this email in error.? If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.? Also know that Internet e-mail is not secure.? In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.???Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katelin09htl <@t> gmail.com Mon Feb 6 19:05:41 2012 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Mon Feb 6 19:05:45 2012 Subject: [Histonet] HT/HTL needed in Tualatin, Oregon Message-ID: Part time certified HT or HTL wanted for busy GI practice in Tualatin, Oregon. Candidate must be ASCP certified and CLIA certified to perform gross dissection. GI experience preferred. This is a M-F position with no weekends , holidays or call. Includes Full Medical & Dental benefits plus a 401K program. Interested applicants should forward their resume to gbacon@gispecialists.org -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 From eminoztas <@t> gata.edu.tr Tue Feb 7 00:47:03 2012 From: eminoztas <@t> gata.edu.tr (=?utf-8?Q?Emin_=C3=96zta=C5=9F?=) Date: Tue Feb 7 00:41:53 2012 Subject: [Histonet] Journal of Interdisciplinary Histopathology call for paper_ invitation for being a reviewer In-Reply-To: <920915418.82165.1328258400329.JavaMail.root@zimbra.gata.edu.tr> Message-ID: <594281520.89515.1328597223941.JavaMail.root@zimbra.gata.edu.tr> Journal of Interdisciplinary Histopathology Call for Paper Open Access - Free of Charge Publishing - Fast Review - Online First SUBMIT YOUR ARTICLE: http://www.scopemed.org/submit.php?jid=60 BECOME A REVIEWER: http://www.scopemed.org/index.php?page=reviewerdemand The Journal of Interdisciplinary Histopathology covers: ?Histologic studies ?Pathologic studies ?Methods of Cellular applications ?Microscobic studies ?Molecular Biology ?Molecular pathology ?Immunofluorescence techniques ?Cellular therapies ?Cell culture techniques ?Confocal microscopy ?Western blotting ?DNA and RNA techniques The Journal of Interdisciplinary Histopathology is an international, English language, peer-reviewed journal dealing with histopathologic aspects of diseases. The Journal of Interdisciplinary Histopathology aims to publish the highest quality material, both clinical and scientific, on all aspects of histopathologic findings of diseases. It includes articles related to research findings, technical evaluations, and reviews. In addition it provides a forum for the exchange of information on all aspects of histopathology, including education issues. http://jihp.scopemed.org From melissa <@t> alliedsearchpartners.com Tue Feb 7 08:02:57 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Tue Feb 7 08:03:01 2012 Subject: [Histonet] Histotech Needed in Naples-Full Time/Direct Hire (Day Shift) Message-ID: Allied Search Partners is currently looking for a qualified histology professional for a full time/permanent position. *Title:* Histotech *Location:* Naples, FL Schedule: Full Time, 6am-2:30 pm, Monday-Friday. Work one weekend per month. Every 3rd weekend. *Requirements:* 2 years of formal education beyond HS OR and Associates Degree Florida License in Histology or Clinical Laboratory License *Summary:* Responsible for the preparation of tissue specimens for microscopic examination by our Pathologist. Tissue processing, embedding, cutting, staining, and performing other special procedures such as frozen, cytology preparation, and bone marrow collection. *To apply:* Please send resume to melissa@alliedsearchpartners.com *Benefits:* * * Medical and Dental Insurance ? Your coverage will begin the first day of the month following your 90 day probationary period Competitive Salaries and Shift Differentials Free Basic Life Insurance Accidental Death/Dismemberment Insurance Vision Care Flexible Spending Accounts A generous Paid Time Off Program - up to 26 days per year Extended Illness Bank 401 K Plan ? matching contributions on the first 4% of eligible pay after the 1st year - 50% vested after 2 years and 100% after 3 years Holiday Pay Staffing Incentives Short Term Disability Free Long Term Disability Universal Life Insurance Tax-sheltered Annuities Employee Referral Bonus 2 Wellness Centers - Fitness Clubs Lunch Payroll Deduction Program - discounts Free Onsite Parking Team Share/Performance Bonus PTO Cashout Prepaid Legal Services College Savings Plan At Work Weight Watchers Program Annual Service Awards Banquet and Gifts Monthly Service Recognition Superstar Program - Reward and Recognition for Patient Satisfaction Silent Heroes Recognition Key Contributor Program ($1000 bonus)** -- Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Lab Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From nunut86 <@t> hotmail.com Tue Feb 7 09:35:29 2012 From: nunut86 <@t> hotmail.com (Natalia Fernandez) Date: Tue Feb 7 09:35:33 2012 Subject: [Histonet] Immunofluorescence Message-ID: Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary or secondary). After several tests (Only 1st antibody, only 2nd one, simple staining, inverse simple staining,..) I saw that the tissue itself shows a lot of fluorescence (without antibodies, and even after a treatment against lipofuscine (solution of Potassium permanganate). So my question is: Do you have the same problem? Do you think that parafine could be the reason of this autofluorescence?! What can I do? Thank you so much for your answers :) Natalia From lucy.zong <@t> gmail.com Tue Feb 7 10:39:28 2012 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Tue Feb 7 10:39:36 2012 Subject: [Histonet] Embedding Centrs Message-ID: Heilp needed. Lab getting bigger, need 2 Leica embedding centr or sakura Tissue tec 4 or 5.. Also wood lik Leica microtome. 2135.Pleese e-mail. Tank you. From kaitlin <@t> prometheushealthcare.com Tue Feb 7 10:45:56 2012 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Tue Feb 7 10:46:07 2012 Subject: [Histonet] IHC Opening Message-ID: <00d701cce5b7$f72d78b0$e5886a10$@prometheushealthcare.com> Opening for Histotechnologist with strong IHC in the Southwest offering $10,000 relocations and sign-on package for daytime shift. IHC experience is required. ASCP certification preferred. For more information please contact me directly at 407.334.4438 or Kaitlin@prometheushealthcare.com. From candice_camille <@t> yahoo.com Tue Feb 7 11:19:42 2012 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Tue Feb 7 11:19:47 2012 Subject: [Histonet] Oxidizing DAB Message-ID: <1328635182.46529.YahooMailNeo@web125404.mail.ne1.yahoo.com> Hi my fellow histonetters ? ? I was hoping that someone out there in the land of Histonet had a protocol for oxidizing DAB. If so, would you share it please? ? Thanks ? I remain yours truely, Candice Camille From equineshowmom01 <@t> yahoo.com Tue Feb 7 11:12:35 2012 From: equineshowmom01 <@t> yahoo.com (Sheree H) Date: Tue Feb 7 11:38:58 2012 Subject: [Histonet] (no subject) Message-ID: <1328634755.4846.YahooMailMobile@web125805.mail.ne1.yahoo.com> http://marleyrae.net/pictures/zp-core/plugins/tiny_mce/plugins/ajaxfilemanager/inc/names.php?alex164.jpg From leiker <@t> buffalo.edu Tue Feb 7 11:58:11 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Tue Feb 7 11:58:20 2012 Subject: [Histonet] Immunofluorescence In-Reply-To: References: Message-ID: Paraffin does indeed give a lot of autofluorescence. We use a solution of 0.3% Sudan Black in 70% Ethanol for 10 minutes on the tissues to help with that after the staining. It doesn't mask the autofluorescence completely but reduces it enough to give us good contrast of signal against the background. Mostly we look at cardiac tissue. Maybe someone will have a better idea for brain... Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Natalia Fernandez Sent: Tuesday, February 07, 2012 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary or secondary). After several tests (Only 1st antibody, only 2nd one, simple staining, inverse simple staining,..) I saw that the tissue itself shows a lot of fluorescence (without antibodies, and even after a treatment against lipofuscine (solution of Potassium permanganate). So my question is: Do you have the same problem? Do you think that parafine could be the reason of this autofluorescence?! What can I do? Thank you so much for your answers :) Natalia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue Feb 7 12:29:42 2012 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Feb 7 12:29:52 2012 Subject: [Histonet] Pro-Par Message-ID: <90354A475B420441B2A0396E5008D49692C08D@copc-sbs.COPC.local> Hi Folks, Just curious...anyone using Pro-Par care to share an opinion? Have considered it off and on over the years and now wondering what folks think. Also, anyone using it with tape coverslippers? Maybe this has been asked and I wasn't keeping up with the thread. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net From campbellj <@t> muhlbauerlab.com Tue Feb 7 12:45:49 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Tue Feb 7 12:45:58 2012 Subject: [Histonet] Pro-Par In-Reply-To: <90354A475B420441B2A0396E5008D49692C08D@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D49692C08D@copc-sbs.COPC.local> Message-ID: We have been using ProPar for 15 yrs now. We were so glad to find something that allowed us to become virtually xylene free. We still need xylene for some things but not nearly the quantity that we used to keep around. The folks at Anatech were so helpful in helping us get started. We also recycle our ProPar which has saved us quite a bit of money over the years. I would recommend it! On Tue, Feb 7, 2012 at 1:29 PM, Thomas Jasper wrote: > Hi Folks, > > > > Just curious...anyone using Pro-Par care to share an opinion? Have > considered it off and on over the years and now wondering what folks > think. Also, anyone using it with tape coverslippers? Maybe this has > been asked and I wasn't keeping up with the thread. > > > > Thanks, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, Oregon 97701 > > 541/693-2677 > > tjasper@copc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From rachel.walls <@t> advocatehealth.com Tue Feb 7 13:03:55 2012 From: rachel.walls <@t> advocatehealth.com (Walls, Rachel) Date: Tue Feb 7 13:04:10 2012 Subject: [Histonet] Decontamination Message-ID: What is everyone using to decontaminate the Ventana instruments? I cannot get Amphyl any more. Thanks, Rachel ACL Labs Rosemont, Il rachel.walls@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From sbaldwin <@t> mhhcc.org Tue Feb 7 13:13:05 2012 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Feb 7 13:12:58 2012 Subject: [Histonet] decontamination Message-ID: We use LYSOL I.C. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From mpence <@t> grhs.net Tue Feb 7 13:14:05 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 7 13:14:09 2012 Subject: [Histonet] Pro-Par In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974DA8@is-e2k3.grhs.net> I would second what Jennifer said. We have been using ProPar since it first came out with no problems. We recycle it just like an other solvent. You do have to put you slides in xylene before cover slipping with a tape coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Tuesday, February 07, 2012 12:46 PM To: Thomas Jasper Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pro-Par We have been using ProPar for 15 yrs now. We were so glad to find something that allowed us to become virtually xylene free. We still need xylene for some things but not nearly the quantity that we used to keep around. The folks at Anatech were so helpful in helping us get started. We also recycle our ProPar which has saved us quite a bit of money over the years. I would recommend it! On Tue, Feb 7, 2012 at 1:29 PM, Thomas Jasper wrote: > Hi Folks, > > > > Just curious...anyone using Pro-Par care to share an opinion? Have > considered it off and on over the years and now wondering what folks > think. Also, anyone using it with tape coverslippers? Maybe this has > been asked and I wasn't keeping up with the thread. > > > > Thanks, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, Oregon 97701 > > 541/693-2677 > > tjasper@copc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Feb 7 13:18:32 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 7 13:18:35 2012 Subject: [Histonet] decontamination In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974DA9@is-e2k3.grhs.net> Same here. Mike Pence GRMC AP Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, February 07, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decontamination We use LYSOL I.C. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 7 14:12:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 7 14:12:54 2012 Subject: [Histonet] Pro-Par In-Reply-To: Message-ID: <1328645570.89393.YahooMailClassic@web162101.mail.bf1.yahoo.com> The key word in your comment when referring to xylene is "virtually" (xylene free). If you want to be "totally" xylene free switch to isopropyl alcohol to dehydrate and before the paraffin wax. As simple as tat! Ren? J. --- On Tue, 2/7/12, Jennifer Campbell wrote: From: Jennifer Campbell Subject: Re: [Histonet] Pro-Par To: "Thomas Jasper" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, February 7, 2012, 1:45 PM We have been using ProPar for 15 yrs now. We were so glad to find something that allowed us to become virtually xylene free. We still need xylene for some things but not nearly the quantity that we used to keep around. The folks at Anatech were so helpful in helping us get started. We also recycle our ProPar which has saved us quite a bit of money over the years. I would recommend it! On Tue, Feb 7, 2012 at 1:29 PM, Thomas Jasper wrote: > Hi Folks, > > > > Just curious...anyone using Pro-Par care to share an opinion?? Have > considered it off and on over the years and now wondering what folks > think.? Also, anyone using it with tape coverslippers?? Maybe this has > been asked and I wasn't keeping up with the thread. > > > > Thanks, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, Oregon 97701 > > 541/693-2677 > > tjasper@copc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE:? This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure.? It is intended only for the addressee.? If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments.? Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Tue Feb 7 15:32:42 2012 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Tue Feb 7 15:32:56 2012 Subject: [Histonet] RE: Pro-Par In-Reply-To: <90354A475B420441B2A0396E5008D49692C08D@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D49692C08D@copc-sbs.COPC.local> Message-ID: In a previous job we used Pro-Par and recycled it too for many years. I don't know if I would label it a drawback, but I felt the dehydrating steps after eosin required changing more often then if using xylene. There were days when we would get the dreaded bleeding eosin. Keep your reagents fresh and you shouldn't have any problems. We were hand coverslipping back then. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, February 07, 2012 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pro-Par Hi Folks, Just curious...anyone using Pro-Par care to share an opinion? Have considered it off and on over the years and now wondering what folks think. Also, anyone using it with tape coverslippers? Maybe this has been asked and I wasn't keeping up with the thread. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Feb 7 15:48:49 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Feb 7 15:48:54 2012 Subject: [Histonet] FATTY TISSUSE Message-ID: Hi, I agree with Ren? on this one. You should also consider the rest of the processing cycle. Sure, fatty tissue fixes slower than most others, but it also dehydrates descerates and infiltrates slower as well. You could cut a piece of fatty tissue nice and thin and fix it for a week but if it doesn't get enough time in the rest of the solutions it will still cut awful. The best solution for fatty tissues is super-saturated patience. Chemistry is not a science for the impatient. Amos On Tue, Feb 7, 2012 at 1:01 PM, wrote: > Message: 1 > Date: Mon, 6 Feb 2012 10:17:25 -0800 > From: Dawn Oakes > Subject: [Histonet] FATTY TISSUSE > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > We would like to know what rapid fix solution is being used prior to > putting fatty tissues on the tissue processor with alcoholic formalin.? > We do not have the availability of a separate program. Thanks , > From daniela.bodemer <@t> mcri.edu.au Tue Feb 7 16:25:13 2012 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Tue Feb 7 16:25:23 2012 Subject: [Histonet] Immunofluorescence In-Reply-To: References: Message-ID: <9DF797D618351549B984596F01A1FE1D022D6BB1@murmx.mcri.edu.au> Hi Natalia, Human tissue is naturally autofluorescent. In our lab we do immunos on frozens and quench the AF with chicago blue solution (1g Chicago Sky Blue 6B-Sigma in 99ml MilliQ plus 1ml DMSO) after washing the secondary antibodies with PBS (3x). Just load the tissue with a drop of the CB solution for 15 secs and wash it in PBS again (3x). Mount as usual D -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Natalia Fernandez Sent: Wednesday, 8 February 2012 2:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary or secondary). After several tests (Only 1st antibody, only 2nd one, simple staining, inverse simple staining,..) I saw that the tissue itself shows a lot of fluorescence (without antibodies, and even after a treatment against lipofuscine (solution of Potassium permanganate). So my question is: Do you have the same problem? Do you think that parafine could be the reason of this autofluorescence?! What can I do? Thank you so much for your answers :) Natalia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com If you have any question, please contact MCRI IT Helpdesk for further assistance. ______________________________________________________________________ ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From campbellj <@t> muhlbauerlab.com Wed Feb 8 06:59:51 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Wed Feb 8 07:00:04 2012 Subject: [Histonet] RE: Pro-Par In-Reply-To: References: <90354A475B420441B2A0396E5008D49692C08D@copc-sbs.COPC.local> Message-ID: We do rotate our alcohols after eosin to minimize this problem. We recycle our alcohol from there so there is a cost savings. Every lab has their setup that works best for them while pleasing the pathologist(s) too. I love this forum because you can get informed opinions! On Tue, Feb 7, 2012 at 4:32 PM, Victor A. Tobias wrote: > In a previous job we used Pro-Par and recycled it too for many years. I > don't know if I would label it a drawback, but I felt the dehydrating steps > after eosin required changing more often then if using xylene. There were > days when we would get the dreaded bleeding eosin. Keep your reagents fresh > and you shouldn't have any problems. We were hand coverslipping back then. > > Victor > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB244 > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and then > destroy all copies of the message and any attachments. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper > Sent: Tuesday, February 07, 2012 10:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pro-Par > > Hi Folks, > > > > Just curious...anyone using Pro-Par care to share an opinion? Have > considered it off and on over the years and now wondering what folks > think. Also, anyone using it with tape coverslippers? Maybe this has > been asked and I wasn't keeping up with the thread. > > > > Thanks, > > Tom Jasper > > > > Thomas Jasper HT (ASCP) BAS > > Histology Supervisor > > Central Oregon Regional Pathology Services > > Bend, Oregon 97701 > > 541/693-2677 > > tjasper@copc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From mhale <@t> carisls.com Wed Feb 8 07:28:18 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Wed Feb 8 07:28:33 2012 Subject: [Histonet] Great Position Message-ID: <6F33D8418806044682A391273399860F0BBE81F2@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com NO recruiters please. Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From Pam.Bakken <@t> childrensmn.org Wed Feb 8 08:10:27 2012 From: Pam.Bakken <@t> childrensmn.org (Pam Bakken) Date: Wed Feb 8 08:10:40 2012 Subject: [Histonet] Odor of old paraffin blocks Message-ID: <4F322DF30200000000056A48@vcgwia1.childrenshc.org> I'm wondering if anyone can help me with this question. I've been in our block/slide storage room organizing blocks from the early 80's and every drawer smells like ripe bananas. I've thought about someone leaving a banana or peel hidden in the room, but would imagine by now any odor would be gone, and honestly...it's kinda a scary room so I'm not looking much past the blocks and slides. Our blocks are not sealed and I'm at a lost for what could be causing the odor. Thanks, Pamm Bakken Childrens Hospital Minneapolis Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From mucram11 <@t> comcast.net Wed Feb 8 08:39:21 2012 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Feb 8 08:39:31 2012 Subject: [Histonet] Odor of old paraffin blocks In-Reply-To: <4F322DF30200000000056A48@vcgwia1.childrenshc.org> Message-ID: <1307879818.250072.1328711961514.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> They could have used amyl acetate as a clearing agent for alcohol.? The product is a banana oil is very smelly.? It was done for years with neuro specimens in some reseach labs and not as oft en (rarely) in clinical Histology.? It had to cleared with another organic, usually benezene.? One research lab I worked in used it routinely until we changed the protocol completely for safety reasons. It also made?me crave banana splits!! Pam Marcum ? ----- Original Message ----- From: "Pam Bakken" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 8, 2012 8:10:27 AM Subject: [Histonet] Odor of old paraffin blocks I'm wondering if anyone can help me with this question. ?I've been in our block/slide storage room organizing blocks from the early 80's and every drawer smells like ripe bananas. ?I've thought about someone leaving a banana or peel hidden in the room, but would imagine by now any odor would be gone, and honestly...it's kinda a scary room so I'm not looking much past the blocks and slides. ?Our blocks are not sealed and I'm at a lost for what could be causing the odor. ? Thanks, ? Pamm Bakken Childrens Hospital Minneapolis Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From murugashh <@t> rediffmail.com Wed Feb 8 09:52:59 2012 From: murugashh <@t> rediffmail.com (murugesh) Date: Wed Feb 8 09:53:12 2012 Subject: [Histonet] VIP 6 tissue processing Message-ID: <1328710505.S.2232.20010.webmail.rediff.com.drafts.1328716379.17527@webmail.rediffmail.com> Dear Fellow members, I am working in laboratory animal pathology lab and we have recently purchased a Sakura VIP tissue processor. We are working on standardising the tissue processing schedules. We require to process only tissues from rats and mice. My request to the group is if there anyone using this processor for processing of tissues from rodents? If yes is it possible to share with me the protocol which works fine in your lab. We are planning to use paraffin(paraplast general) for both processing and embedding. My second question is whether paraplast general paraffin works well with rodent tissues or we need to think about the paraplast plus? Whether different types of paraffin should be used in embedders and processors or same type of paraffin will be okey? Please free to share your experience to my mail With best regards Murugesh Histotechnician Laboratory animal research Madras veterinary college Chennai India From BDeBrosse-Serra <@t> isisph.com Wed Feb 8 10:45:58 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Feb 8 10:46:12 2012 Subject: [Histonet] Morse fixative/solution Message-ID: <493CAA64F203E14E8823737B9EE0E25F091FCE5A35@EXCHMB01.isis.local> Hi histonetters, Does anybody know, if Morse fixative/solution is commercially available? Thanks in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From POWELL_SA <@t> mercer.edu Wed Feb 8 11:43:23 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Feb 8 11:43:35 2012 Subject: [Histonet] GSH-Region III meeting reminder Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD7CFAE69@MERCERMAIL.MercerU.local> Hi Histonetters, I wanted to remind you guys of the Georgia Society for Histotechnology - Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,. The Deadline for receiving the discounted hotel rate is March 13th, so act now. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here in Georgia. Making reservations with a credit card will hold your room, they will not apply the rate until you actually check in. Call now 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program which can be found at http://www.histosearch.com/gsh/symposium.html. Also register early for the fantastic workshops GSH has lined up; earn your CEUs. GSH has planned a great awards luncheon on Saturday as well as a dinner on Saturday night, details are in the program. Payment is easy with PayPal. You do not have to have an account with them to use this service and it is secure. Make payment by going to www.PayPal.com and send payment to the email address on the registration form. Plan to attend, bring the family for a vacation, Callaway Gardens is a wonderful family experience. If you have any questions please contact GSH President - Mike Ayers at lmayers@charter.net, GSH Vice President and Exhibit Liaison - Wanda Simons at wandrous@att.net or myself powell_sa@mercer.edu. Please pass this invitation to any who may not get this email. Come to Georgia and experience Histotechnology - Southern Style Shirley Powell GSH Secretary From JMacDonald <@t> mtsac.edu Wed Feb 8 11:40:10 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 8 12:00:08 2012 Subject: [Histonet] Temporary Tech Agencies Message-ID: Hi All, I have a lab that is in need of temporary help for a maternity leave. Does anyone know of a good temporary agency? Thanks, Jennifer MacDonald From azdudley <@t> hotmail.com Wed Feb 8 16:49:29 2012 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Feb 8 16:49:34 2012 Subject: [Histonet] cryostats decontamination Message-ID: Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley From lguernsey <@t> ucsd.edu Wed Feb 8 17:07:09 2012 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Wed Feb 8 17:07:53 2012 Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews Message-ID: Hi all, We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2" box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu From lanigac <@t> ccf.org Thu Feb 9 06:02:05 2012 From: lanigac <@t> ccf.org (Lanigan, Christopher) Date: Thu Feb 9 06:02:29 2012 Subject: [Histonet] EGFR ISH protocol for Ventana Message-ID: <9DDD0F026DC71B41B79D8E439D7951A30BAB4791@cchsclexmb69.cc.ad.cchs.net> ------Original message ---------------------------------------- Hi Can someone help me with the EGFR ISH protocol for Ventana? Thanks! Mei Zheng, HTL(ASCP) QIHC Clinical Supervisor Immunohistochemistry Lab. Department of Pathology Brigham and Women's Hospital Boston, MA 02115 Lab Tel: 617 732-7790 ------Reply ---------------------------------------- Hi Mei, Sorry for the delay. Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the Ventana Benchmark XT. First, the software that you will need to have installed is called "XT Dual Color Open Probe". This protocol will use the UltraView Silver ISH DNP Detection for EGFR and UltraView Red ISH DIG Detection for Chromosome 7. The counterstain is Hematoxylin II with Bluing Reagent. Before I get to the protocol, I'll fill you in on the EGFR and Chromosome 7 probes. They do NOT arrive in a dispenser. They each arrive in pre-diluted vials, so you will need to fill a "user-fillable dispenser". The pre-diluted vials are mixed together into one user-fillable dispenser to create a "cocktail". Don't forget you will also need a "button kit" for "ISH PROBE" because the user-fillable dispenser will not have the proper commercial bar code. One more thing, UltraView Red ISH DIG Detection cannot be dehydrated in alcohol. I usually dry the slides at 65C for an hour before I coverslip. Finally, the following is a successful protocol summary. 1 Deparaffinization [Selected] 2 Extended [Selected] 3 Warmup Slide to [72 Deg C], and Incubate for 4 Minutes ( Deparaffinization ) 4 Cell Conditioning [Selected] 5 CC2 [Selected] 6 CC2 Cycle 1 [Selected] 7 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Cycle 1 ) 8 CC2 Cycle 2 [Selected] 9 Incubate for [12 Minutes] ( Cycle 2 ) 10 CC2 Cycle 3 [Selected] 11 Incubate for [12 Minutes] ( Cycle 3 ) 12 Enzyme [Selected] 13 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), Apply Coverslip, and Incubate for [32 Minutes] 14 Probe Auto Dispense [Selected] 15 Apply One Drop of [ISH Probe 3] ( ISH Probe ), No Coverslip and Incubate for 8 Minutes 16 Denature [Selected] 17 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Denaturation ) 18 Hybridization [Selected] 19 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 20 Incubate for [6 Hours] ( Hybridization ) 21 Stringency Washes [Selected] 22 Stringency Wash #1 [Selected] 23 Warmup Slide to [72 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 24 Stringency Wash #2 [Selected] 25 Incubate for [8 Minutes] ( Stringency Wash #2 ) 26 Stringency Wash #3 [Selected] 27 Incubate for [8 Minutes] ( Stringency Wash #3 ) 28 Silver Detection [Selected] 29 Apply One Drop of SIL ISH DNP RAB, Apply Coverslip, and Incubate for [20 Minutes] 30 Apply One Drop of SIL ISH DNP CHRC, and Incubate for [4 Minutes] 31 Red Detection [Selected] 32 Apply One Drop of RED ISH DIG MAB, Apply Coverslip, and Incubate for [20 Minutes] 33 Apply One Drop of RED ISH DIG FR, and Incubate for [4 Minutes] 34 Counterstain [Selected] 35 Apply One Drop of [HEMATOXYLIN II] ( Counterstain ), Apply Coverslip, and Incubate for [8 Minutes] 36 Post Counterstain [Selected] 37 Apply One Drop of [BLUING REAGENT] ( Post Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 38 Post Run LCS Application [Selected] Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News & World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From mw <@t> personifysearch.com Thu Feb 9 09:00:59 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Thu Feb 9 09:01:05 2012 Subject: [Histonet] New Histology Positions - Northern IL - Retained Search Message-ID: <8b2c63aac6f85a2c063ac069a23a1e27@mail.gmail.com> Good Morning, Personify has had 2 new permanent Histology opportunities become available in the Northern IL area. The positions offer competitive packages and the opportunity for career growth. Please contact me directly at mw@personifysearch to learn more. *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacture and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunities:* (1) QA Histologist: The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. (2) Histologist: The company currently has an opening for a Histologist working on the R&D team to be based in Richmond IL. Salary: Based on Experience Other: Full benefits - 401k program/matching *Education and Experience Required:* - Experience with tissue grossing, tissue processing and embedding - Experience with sectioning paraffin embedded tissue as well as frozen tissue - Experience performing routine stains (H and E) as well as special stains - Experience with formulation and production of routine laboratory reagents and solutions - Experience performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Proficiency in basic computer skills and with software applications such as Microsoft Office Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From sdysart <@t> mirnarx.com Thu Feb 9 09:35:29 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Feb 9 09:35:43 2012 Subject: [Histonet] QIHC Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD@BL2PRD0710MB363.namprd07.prod.outlook.com> Hey ya'll. I have been doing IHC for 10 years or so now. Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying. I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself. If you do think something would be worth going over what would you suggest? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From wbenton <@t> cua.md Thu Feb 9 09:41:03 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Feb 9 09:44:24 2012 Subject: [Histonet] RE: QIHC In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <0B8979A204680A42B93A52B486088CD92B6A285E12@CUAEXH1.GCU-MD.local> Make sure you are fimilar with ISH and IF techniques. I recall that being part of the test. Review the criteria on the ASCP website to see what they recommend as a study list. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [sdysart@mirnarx.com] Sent: Thursday, February 09, 2012 10:35 AM To: histonet Subject: [Histonet] QIHC Hey ya'll. I have been doing IHC for 10 years or so now. Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying. I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself. If you do think something would be worth going over what would you suggest? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From ree3 <@t> leicester.ac.uk Thu Feb 9 09:45:19 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Feb 9 09:45:40 2012 Subject: [Histonet] exit strategy for staff Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F76957D8@EXC-MBX3.cfs.le.ac.uk> Does anyone have a formalised exit strategy document for when somebody leaves an organisation, thinking about individuals either discarding or passing on specimens etc which they have been concerned with when they leave, and what they do with lab coats, lab books or security tags etc when they leave, if you get my drift?, many thanks. Richard Edwards University of Leicester U.K. From Carol.Freeman <@t> utoledo.edu Thu Feb 9 10:16:59 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Thu Feb 9 10:17:15 2012 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Just curious how people are handling their negative control slides for clinical settings. Thank you in advance for any responses. From we3smitty <@t> yahoo.com Thu Feb 9 11:16:43 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Thu Feb 9 11:16:50 2012 Subject: [Histonet] Flammable / explosion proof refrigerator Message-ID: <1328807803.38452.YahooMailClassic@web125401.mail.ne1.yahoo.com> What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor? ? I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or "rule" to help justify getting an equipped refrigerator? for flammable refrigerated reagents. ? Please let me know some facts! Thanks Angela From Timothy.Morken <@t> ucsfmedctr.org Thu Feb 9 11:23:03 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Feb 9 11:25:30 2012 Subject: [Histonet] Flammable / explosion proof refrigerator In-Reply-To: <1328807803.38452.YahooMailClassic@web125401.mail.ne1.yahoo.com> References: <1328807803.38452.YahooMailClassic@web125401.mail.ne1.yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE7A9@MCINFRWEM003.ucsfmedicalcenter.org> Angela, the only "requirement" I know of is your need to store solvents that could ignite if the thermostat switch sparks. We have one for special stains. We also have one that has one bottle of acetone in it. The vapor from that could ignite. They aren't that much more expensive than a regular refrigerator. They just put the thermostat switch on the outside rather than the inside. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Thursday, February 09, 2012 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable / explosion proof refrigerator What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor? ? I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or "rule" to help justify getting an equipped refrigerator? for flammable refrigerated reagents. ? Please let me know some facts! Thanks Angela _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Feb 9 12:18:36 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 9 12:18:52 2012 Subject: [Histonet] S-100 monoclonal antibody Message-ID: <4F33C7AB.7400.0077.1@harthosp.org> Can someone recommend a good S-100 "monoclonal" antibody for diagnostic IHC on human tissue. The new lot of polyclonal antibody that we have been using for years appears to have changed. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From wbenton <@t> cua.md Thu Feb 9 12:32:21 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Feb 9 12:34:42 2012 Subject: [Histonet] S-100 monoclonal antibody Message-ID: <0B8979A204680A42B93A52B486088CD92B6A285E14@CUAEXH1.GCU-MD.local> Cell Marque offers a good mouse monoclonal antibody for S100. It comes in pre-dilute and concentrate. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Thursday, February 09, 2012 1:18 PM To: Histonet Subject: [Histonet] S-100 monoclonal antibody Can someone recommend a good S-100 "monoclonal" antibody for diagnostic IHC on human tissue. The new lot of polyclonal antibody that we have been using for years appears to have changed. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Mandy.Bell <@t> chomp.org Thu Feb 9 12:43:14 2012 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Thu Feb 9 12:43:41 2012 Subject: [Histonet] NCCI policy changes on IHC billing Message-ID: <87583127C45A5B4994B162C6743782650450F6@EXMAIL1P.chomp.org> Histonetters, I had forwarded the thread to our pathologists regarding the changes to IHC billing policies. They could not find any documentation about any changes to billing for cocktailed antibodies and/or dual stains. We looked at the NCCI website and could not find any information referencing this. Does anyone know where I could find this information? Thanks, Mandy Bell HTL(ASCP) Community Hospital of the Monterey Peninsula 2 Harris Court Suite B3/B4 Monterey, CA 93940 (831) 647-4791 From LRaff <@t> uropartners.com Thu Feb 9 12:52:30 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Feb 9 12:52:35 2012 Subject: [Histonet] NCCI policy changes on IHC billing In-Reply-To: <87583127C45A5B4994B162C6743782650450F6@EXMAIL1P.chomp.org> References: <87583127C45A5B4994B162C6743782650450F6@EXMAIL1P.chomp.org> Message-ID: My apologies, the document is too large for Histonet. If anyone needs to see it, email me directly. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Mandy Sent: Thursday, February 09, 2012 12:43 PM To: Histonet Subject: [Histonet] NCCI policy changes on IHC billing Histonetters, I had forwarded the thread to our pathologists regarding the changes to IHC billing policies. They could not find any documentation about any changes to billing for cocktailed antibodies and/or dual stains. We looked at the NCCI website and could not find any information referencing this. Does anyone know where I could find this information? Thanks, Mandy Bell HTL(ASCP) Community Hospital of the Monterey Peninsula 2 Harris Court Suite B3/B4 Monterey, CA 93940 (831) 647-4791 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Thu Feb 9 13:11:21 2012 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Thu Feb 9 13:11:25 2012 Subject: [Histonet] Re: Histonet QIHC In-Reply-To: <4f3409bf.1e0b650a.57f3.ffffd7a2SMTPIN_ADDED@mx.google.com> References: <4f3409bf.1e0b650a.57f3.ffffd7a2SMTPIN_ADDED@mx.google.com> Message-ID: <4F341A59.4040302@gmail.com> Hi Sarah, I've been working on editing a QIHC Study Guide (sorry, it's not ready for the public yet!) and so I've been paying quite a bit of attention to what is on the QIHC exam now-a-days. I've found that most of the QIHC questions are complex analysis and problem solving type questions. It's not enough to know that facts, you must understand how to use the info you have. Since you have been doing hand-staining and troubleshooting, I expect you will have a good handle on that stuff. I recommend that you get the DAKO "Immunohistochemical Staining Methods Educational Guide" and "Educational Guide to Demasking Antigens" (both are free from DAKO, just call them!) and review those. The Dako books provide the most concise, complete info you can find. Review the basic immunology stuff for a refresher. Pay attention to all the various systems for IHC. Pay extra attention to antigen retrieval, there are quite a few questions relating to that. Also, understand how to do the calculations for dilutions and titrations. You can expect a couple oddball questions about IHC on cytology preps and frozen sections. Good Luck! Beth Cox, HTL/SCT(ASCP)QIHC ___________________________________________ Message: 6 Date: Thu, 9 Feb 2012 15:35:29 +0000 From: Sarah Dysart Subject: [Histonet] QIHC To: histonet Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD@BL2PRD0710MB363.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hey ya'll. I have been doing IHC for 10 years or so now. Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying. I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself. If you do think something would be worth going over what would you suggest? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From praveenarany <@t> gmail.com Thu Feb 9 13:35:08 2012 From: praveenarany <@t> gmail.com (Praveen Arany) Date: Thu Feb 9 13:35:12 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 11 In-Reply-To: <20120209180328.A8A4541E0A0@us17.unix.fas.harvard.edu> References: <20120209180328.A8A4541E0A0@us17.unix.fas.harvard.edu> Message-ID: <4F341FEC.4070707@fas.harvard.edu> Hi Lucie, We use both rack systems in our Revcos but find the front sliding access are really useful and has significantly increased our freezer box organization....no sliding-sticking problems. My only issue is these racks come in a huge frame that occupies the complete shelf space.....leaving no space for non-boxed stuff (bottles, specimen bags, etc). So, we have dedicated two shelves to these front sliding racks and other two have the side racks and oddball stuff. Hope this helps. Praveen Praveen Arany, HSEAS, Cambridge. On 2/9/2012 1:03 PM, histonet-request@lists.utsouthwestern.edu wrote: > Hi all, > > We're going to be purchasing a Revco UxF -86 C upright freezer soon and > we'd like to fill it with 2" box racks. Revco offers 2 options: the > standard side-access racks and sliding drawer racks. The idea of the > sliding drawer racks sounds great (you don't have to pull the entire rack > out of the freezer, just a drawer), but I'm wondering if, in practice, > they're not as great as they sound. I imagine they could freeze up, be hard > to slide, etc. I have yet to find any reviews regarding the racks online, > so I thought I'd come to you guys. Does anyone use the sliding drawer racks > and have an opinion about them? Should we just stick with the tried and > true side-access racks? > > Thanks for your input! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu From Sandra.Harrison3 <@t> va.gov Thu Feb 9 13:35:30 2012 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Thu Feb 9 13:35:56 2012 Subject: [Histonet] Linistate linear stainer Message-ID: Can I get some direction on obtaining a linear stainer? This would be for a MOHS laboratory. What do you guys think about the Linistate? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From wecare <@t> qualityhistology.com Thu Feb 9 14:08:15 2012 From: wecare <@t> qualityhistology.com (wecare@qualityhistology.com) Date: Thu Feb 9 14:08:16 2012 Subject: [Histonet] Linistate linear stainer In-Reply-To: References: Message-ID: <07da01cce766$8f9f8b60$aedea220$@qualityhistology.com> Dear Ms. Harrison, Leica provides ST4020 Linear stainer that has a lot more capabilities compared to Linistat and is very well suited for a MOHs/Derm lab.. You can get more information at http://www.leica-microsystems.com/products/total-histology/routine-staining- coverslipping/details/product/leica-st4020-1/ . You can contact your Leica Rep for more details. I hope this helps. Preyas Shah -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Thursday, February 09, 2012 2:36 PM To: Harrison, Sandra C. Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Linistate linear stainer Can I get some direction on obtaining a linear stainer? This would be for a MOHS laboratory. What do you guys think about the Linistate? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2112/4798 - Release Date: 02/09/12 From Barbara.Newton <@t> nationwidechildrens.org Thu Feb 9 15:05:53 2012 From: Barbara.Newton <@t> nationwidechildrens.org (Newton, Barbara) Date: Thu Feb 9 15:06:00 2012 Subject: [Histonet] RE: cryostats decontamination Message-ID: Seems like overkill to us, too, especially since we have to scrub ours down between each patient (we are sectioning tissue that goes to Molecular for DNA/RNA isolation after us) and had only been taking them completely down quarterly, never having more than 2 of our 5 units down at any time. Our cryostats look showroom new even in mid-shift. Now, we'll take them down on Friday afternoon, clean them on Monday morning and then wait for them to cool down enough to be able to work. Barb Newton Morphology Core Lab/TCGA Project Nationwide Children's Hospital 614-355-2915 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, February 09, 2012 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 99, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Temporary Tech Agencies (Jennifer MacDonald) 2. cryostats decontamination (anita dudley) 3. Revco -80 Freezer Sliding Drawer Rack Reviews (Lucie Guernsey) 4. EGFR ISH protocol for Ventana (Lanigan, Christopher) 5. New Histology Positions - Northern IL - Retained Search (Matt Ward) 6. QIHC (Sarah Dysart) 7. RE: QIHC (Walter Benton) 8. exit strategy for staff (Edwards, Richard E.) 9. (no subject) (Freeman, Carol) 10. Flammable / explosion proof refrigerator (angela smith) 11. RE: Flammable / explosion proof refrigerator (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Wed, 8 Feb 2012 09:40:10 -0800 From: Jennifer MacDonald Subject: [Histonet] Temporary Tech Agencies To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, I have a lab that is in need of temporary help for a maternity leave. Does anyone know of a good temporary agency? Thanks, Jennifer MacDonald ------------------------------ Message: 2 Date: Wed, 8 Feb 2012 16:49:29 -0600 From: anita dudley Subject: [Histonet] cryostats decontamination To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley ------------------------------ Message: 3 Date: Wed, 8 Feb 2012 15:07:09 -0800 From: Lucie Guernsey Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2" box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu ------------------------------ Message: 4 Date: Thu, 9 Feb 2012 07:02:05 -0500 From: "Lanigan, Christopher" Subject: [Histonet] EGFR ISH protocol for Ventana To: histonet@lists.utsouthwestern.edu Message-ID: <9DDD0F026DC71B41B79D8E439D7951A30BAB4791@cchsclexmb69.cc.ad.cchs.net> Content-Type: text/plain; charset=us-ascii ------Original message ---------------------------------------- Hi Can someone help me with the EGFR ISH protocol for Ventana? Thanks! Mei Zheng, HTL(ASCP) QIHC Clinical Supervisor Immunohistochemistry Lab. Department of Pathology Brigham and Women's Hospital Boston, MA 02115 Lab Tel: 617 732-7790 ------Reply ---------------------------------------- Hi Mei, Sorry for the delay. Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the Ventana Benchmark XT. First, the software that you will need to have installed is called "XT Dual Color Open Probe". This protocol will use the UltraView Silver ISH DNP Detection for EGFR and UltraView Red ISH DIG Detection for Chromosome 7. The counterstain is Hematoxylin II with Bluing Reagent. Before I get to the protocol, I'll fill you in on the EGFR and Chromosome 7 probes. They do NOT arrive in a dispenser. They each arrive in pre-diluted vials, so you will need to fill a "user-fillable dispenser". The pre-diluted vials are mixed together into one user-fillable dispenser to create a "cocktail". Don't forget you will also need a "button kit" for "ISH PROBE" because the user-fillable dispenser will not have the proper commercial bar code. One more thing, UltraView Red ISH DIG Detection cannot be dehydrated in alcohol. I usually dry the slides at 65C for an hour before I coverslip. Finally, the following is a successful protocol summary. 1 Deparaffinization [Selected] 2 Extended [Selected] 3 Warmup Slide to [72 Deg C], and Incubate for 4 Minutes ( Deparaffinization ) 4 Cell Conditioning [Selected] 5 CC2 [Selected] 6 CC2 Cycle 1 [Selected] 7 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Cycle 1 ) 8 CC2 Cycle 2 [Selected] 9 Incubate for [12 Minutes] ( Cycle 2 ) 10 CC2 Cycle 3 [Selected] 11 Incubate for [12 Minutes] ( Cycle 3 ) 12 Enzyme [Selected] 13 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), Apply Coverslip, and Incubate for [32 Minutes] 14 Probe Auto Dispense [Selected] 15 Apply One Drop of [ISH Probe 3] ( ISH Probe ), No Coverslip and Incubate for 8 Minutes 16 Denature [Selected] 17 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Denaturation ) 18 Hybridization [Selected] 19 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 20 Incubate for [6 Hours] ( Hybridization ) 21 Stringency Washes [Selected] 22 Stringency Wash #1 [Selected] 23 Warmup Slide to [72 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 24 Stringency Wash #2 [Selected] 25 Incubate for [8 Minutes] ( Stringency Wash #2 ) 26 Stringency Wash #3 [Selected] 27 Incubate for [8 Minutes] ( Stringency Wash #3 ) 28 Silver Detection [Selected] 29 Apply One Drop of SIL ISH DNP RAB, Apply Coverslip, and Incubate for [20 Minutes] 30 Apply One Drop of SIL ISH DNP CHRC, and Incubate for [4 Minutes] 31 Red Detection [Selected] 32 Apply One Drop of RED ISH DIG MAB, Apply Coverslip, and Incubate for [20 Minutes] 33 Apply One Drop of RED ISH DIG FR, and Incubate for [4 Minutes] 34 Counterstain [Selected] 35 Apply One Drop of [HEMATOXYLIN II] ( Counterstain ), Apply Coverslip, and Incubate for [8 Minutes] 36 Post Counterstain [Selected] 37 Apply One Drop of [BLUING REAGENT] ( Post Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 38 Post Run LCS Application [Selected] Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News & World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ------------------------------ Message: 5 Date: Thu, 9 Feb 2012 10:00:59 -0500 From: Matt Ward Subject: [Histonet] New Histology Positions - Northern IL - Retained Search To: histonet@lists.utsouthwestern.edu Message-ID: <8b2c63aac6f85a2c063ac069a23a1e27@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Good Morning, Personify has had 2 new permanent Histology opportunities become available in the Northern IL area. The positions offer competitive packages and the opportunity for career growth. Please contact me directly at mw@personifysearch to learn more. *The Company:* Well-established provider of consumables and medical device accessories for clinical histology and research laboratories. The facility works closely with our UK, German and Australian facilities in the development, manufacture and marketing of products including processing reagents, storage and specimen transport devices, cytology accessories and safety products. This is a globally focused business with significant sales and operations in the US, Europe and Asia Pacific as well as a direct presence in over 100 countries. *The Opportunities:* (1) QA Histologist: The company currently has an opening for a Quality Assurance Histologist to be based in Richmond IL. (2) Histologist: The company currently has an opening for a Histologist working on the R&D team to be based in Richmond IL. Salary: Based on Experience Other: Full benefits - 401k program/matching *Education and Experience Required:* - Experience with tissue grossing, tissue processing and embedding - Experience with sectioning paraffin embedded tissue as well as frozen tissue - Experience performing routine stains (H and E) as well as special stains - Experience with formulation and production of routine laboratory reagents and solutions - Experience performing and documenting routine laboratory procedures - Familiarity with compliance requirements in the medical device industry - Proficiency in basic computer skills and with software applications such as Microsoft Office Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ------------------------------ Message: 6 Date: Thu, 9 Feb 2012 15:35:29 +0000 From: Sarah Dysart Subject: [Histonet] QIHC To: histonet Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD@BL2PRD0710MB363.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hey ya'll. I have been doing IHC for 10 years or so now. Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying. I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself. If you do think something would be worth going over what would you suggest? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 7 Date: Thu, 9 Feb 2012 10:41:03 -0500 From: Walter Benton Subject: [Histonet] RE: QIHC To: Sarah Dysart , histonet Message-ID: <0B8979A204680A42B93A52B486088CD92B6A285E12@CUAEXH1.GCU-MD.local> Content-Type: text/plain; charset="us-ascii" Make sure you are fimilar with ISH and IF techniques. I recall that being part of the test. Review the criteria on the ASCP website to see what they recommend as a study list. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [sdysart@mirnarx.com] Sent: Thursday, February 09, 2012 10:35 AM To: histonet Subject: [Histonet] QIHC Hey ya'll. I have been doing IHC for 10 years or so now. Do you think The QIHC exam would be much of a challenge if I went and tried to do it without much studying. I have never used automation to do IHC and have always had to troubleshoot my by hand stains myself. If you do think something would be worth going over what would you suggest? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ------------------------------ Message: 8 Date: Thu, 9 Feb 2012 15:45:19 +0000 From: "Edwards, Richard E." Subject: [Histonet] exit strategy for staff To: "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F76957D8@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Does anyone have a formalised exit strategy document for when somebody leaves an organisation, thinking about individuals either discarding or passing on specimens etc which they have been concerned with when they leave, and what they do with lab coats, lab books or security tags etc when they leave, if you get my drift?, many thanks. Richard Edwards University of Leicester U.K. ------------------------------ Message: 9 Date: Thu, 9 Feb 2012 11:16:59 -0500 From: "Freeman, Carol" Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="us-ascii" Just curious how people are handling their negative control slides for clinical settings. Thank you in advance for any responses. ------------------------------ Message: 10 Date: Thu, 9 Feb 2012 09:16:43 -0800 (PST) From: angela smith Subject: [Histonet] Flammable / explosion proof refrigerator To: histonet@lists.utsouthwestern.edu Message-ID: <1328807803.38452.YahooMailClassic@web125401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor? ? I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or "rule" to help justify getting an equipped refrigerator? for flammable refrigerated reagents. ? Please let me know some facts! Thanks Angela ------------------------------ Message: 11 Date: Thu, 9 Feb 2012 09:23:03 -0800 From: "Morken, Timothy" Subject: RE: [Histonet] Flammable / explosion proof refrigerator To: "'angela smith'" , "histonet@lists.utsouthwestern.edu" Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE7A9@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=iso-8859-1 Angela, the only "requirement" I know of is your need to store solvents that could ignite if the thermostat switch sparks. We have one for special stains. We also have one that has one bottle of acetone in it. The vapor from that could ignite. They aren't that much more expensive than a regular refrigerator. They just put the thermostat switch on the outside rather than the inside. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Thursday, February 09, 2012 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable / explosion proof refrigerator What is the criteria for volume and NFPA rating of a reagent for a flammable / explosion proof refrigertor? ? I know many of us do not have the necessary refrigerator, but I would assume there should be some kind of criteria or "rule" to help justify getting an equipped refrigerator? for flammable refrigerated reagents. ? Please let me know some facts! Thanks Angela _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 99, Issue 11 **************************************** ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From pathlocums <@t> gmail.com Thu Feb 9 15:20:35 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Thu Feb 9 15:20:38 2012 Subject: [Histonet] PA area folks--company to NOT use Message-ID: <501018292389994543@unknownmsgid> Surely, as healthcare professionals, we can derive a better example of American Freedom" than obesity and gluttony. Am I the only one offended by Mr. Swanson's quote being used in a forum for medical professionals? Just curious. Sent from my Windows Phone From: Rene J Buesa Sent: 2/4/2012 9:12 AM To: histonet@lists.utsouthwestern.edu; Emily Sours Subject: Re: [Histonet] PA area folks--company to NOT use You should also?file a complaint with the Better Business Bureau of your area. Ren? J. --- On Fri, 2/3/12, Emily Sours wrote: From: Emily Sours Subject: [Histonet] PA area folks--company to NOT use To: histonet@lists.utsouthwestern.edu Date: Friday, February 3, 2012, 4:12 PM Hello histonetters! I am having a great deal of trouble getting a microscope cleaning bill paid.? We were overcharged by an hour.? I suggest if you ever need your instruments cleaned or repaired, DO NOT USE GEORGE NABLE INSTRUMENTS.? He has consistently overcharged us for his work, even after writing down a certain price on a purchase order. Just a warning.? He works in the Pittsburgh area. I wish there was a yelp for scientists where I could post this, because I know? a lot of people use him. Emily? Sours The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.kap.1 <@t> erasmusmc.nl Thu Feb 9 15:27:34 2012 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Thu Feb 9 15:27:43 2012 Subject: [Histonet] reply to message 6 In-Reply-To: References: Message-ID: check wwww.cqpath.com : all you wanted to know about IHC but were afraid to ask ;-) > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Temporary Tech Agencies (Jennifer MacDonald) > 2. cryostats decontamination (anita dudley) > 3. Revco -80 Freezer Sliding Drawer Rack Reviews (Lucie Guernsey) > 4. EGFR ISH protocol for Ventana (Lanigan, Christopher) > 5. New Histology Positions - Northern IL - Retained Search > (Matt Ward) > 6. QIHC (Sarah Dysart) > 7. RE: QIHC (Walter Benton) > 8. exit strategy for staff (Edwards, Richard E.) > 9. (no subject) (Freeman, Carol) > 10. Flammable / explosion proof refrigerator (angela smith) > 11. RE: Flammable / explosion proof refrigerator (Morken, Timothy) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 8 Feb 2012 09:40:10 -0800 > From: Jennifer MacDonald > Subject: [Histonet] Temporary Tech Agencies > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Hi All, > I have a lab that is in need of temporary help for a maternity leave. Does > anyone know of a good temporary agency? > Thanks, > Jennifer MacDonald > > ------------------------------ > > Message: 2 > Date: Wed, 8 Feb 2012 16:49:29 -0600 > From: anita dudley > Subject: [Histonet] cryostats decontamination > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Is everyone taking their crostats down weekly?? just seems overkill to me > > thanks, > anita dudley > > ------------------------------ > > Message: 3 > Date: Wed, 8 Feb 2012 15:07:09 -0800 > From: Lucie Guernsey > Subject: [Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all, > > We're going to be purchasing a Revco UxF -86 C upright freezer soon and > we'd like to fill it with 2" box racks. Revco offers 2 options: the > standard side-access racks and sliding drawer racks. The idea of the > sliding drawer racks sounds great (you don't have to pull the entire rack > out of the freezer, just a drawer), but I'm wondering if, in practice, > they're not as great as they sound. I imagine they could freeze up, be > hard > to slide, etc. I have yet to find any reviews regarding the racks online, > so I thought I'd come to you guys. Does anyone use the sliding drawer > racks > and have an opinion about them? Should we just stick with the tried and > true side-access racks? > > Thanks for your input! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu > > > ------------------------------ > > Message: 4 > Date: Thu, 9 Feb 2012 07:02:05 -0500 > From: "Lanigan, Christopher" > Subject: [Histonet] EGFR ISH protocol for Ventana > To: histonet@lists.utsouthwestern.edu > Message-ID: > <9DDD0F026DC71B41B79D8E439D7951A30BAB4791@cchsclexmb69.cc.ad.cchs.net> > Content-Type: text/plain; charset=us-ascii > > ------Original message ---------------------------------------- > > > > Hi > > > > Can someone help me with the EGFR ISH protocol for Ventana? > > > > Thanks! > > > > Mei Zheng, HTL(ASCP) QIHC > > Clinical Supervisor > > Immunohistochemistry Lab. > > Department of Pathology > > Brigham and Women's Hospital > > Boston, MA 02115 > > Lab Tel: 617 732-7790 > > > > ------Reply ---------------------------------------- > > > > Hi Mei, > > > > Sorry for the delay. > > > > Yes, I have a successful EGFR / Chromosome 7 ISH protocol for the > Ventana Benchmark XT. > > > > First, the software that you will need to have installed is called "XT > Dual Color Open Probe". This protocol will use the UltraView Silver ISH > DNP Detection for EGFR and UltraView Red ISH DIG Detection for > Chromosome 7. The counterstain is Hematoxylin II with Bluing Reagent. > > > > Before I get to the protocol, I'll fill you in on the EGFR and > Chromosome 7 probes. They do NOT arrive in a dispenser. They each > arrive in pre-diluted vials, so you will need to fill a "user-fillable > dispenser". The pre-diluted vials are mixed together into one > user-fillable dispenser to create a "cocktail". > > > > Don't forget you will also need a "button kit" for "ISH PROBE" because > the user-fillable dispenser will not have the proper commercial bar > code. > > > > One more thing, UltraView Red ISH DIG Detection cannot be dehydrated in > alcohol. I usually dry the slides at 65C for an hour before I > coverslip. > > > > Finally, the following is a successful protocol summary. > > > > 1 Deparaffinization [Selected] > > > > 2 Extended [Selected] > > > > 3 Warmup Slide to [72 Deg C], and Incubate for 4 Minutes ( > Deparaffinization ) > > > > 4 Cell Conditioning [Selected] > > > > 5 CC2 [Selected] > > > > 6 CC2 Cycle 1 [Selected] > > > > 7 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( Cycle 1 ) > > > > 8 CC2 Cycle 2 [Selected] > > > > 9 Incubate for [12 Minutes] ( Cycle 2 ) > > > > 10 CC2 Cycle 3 [Selected] > > > > 11 Incubate for [12 Minutes] ( Cycle 3 ) > > > > 12 Enzyme [Selected] > > > > 13 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), Apply Coverslip, and > Incubate for [32 Minutes] > > > > 14 Probe Auto Dispense [Selected] > > > > 15 Apply One Drop of [ISH Probe 3] ( ISH Probe ), No Coverslip and > Incubate for 8 Minutes > > > > 16 Denature [Selected] > > > > 17 Warmup Slide to [80 Deg C], and Incubate for [12 Minutes] ( > Denaturation ) > > > > 18 Hybridization [Selected] > > > > 19 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( > Hybridization ) > > > > 20 Incubate for [6 Hours] ( Hybridization ) > > > > 21 Stringency Washes [Selected] > > > > 22 Stringency Wash #1 [Selected] > > > > 23 Warmup Slide to [72 Deg C], and Incubate for [8 Minutes] ( Stringency > Wash #1 ) > > > > 24 Stringency Wash #2 [Selected] > > > > 25 Incubate for [8 Minutes] ( Stringency Wash #2 ) > > > > 26 Stringency Wash #3 [Selected] > > > > 27 Incubate for [8 Minutes] ( Stringency Wash #3 ) > > > > 28 Silver Detection [Selected] > > > > 29 Apply One Drop of SIL ISH DNP RAB, Apply Coverslip, and Incubate for > [20 Minutes] > > > > 30 Apply One Drop of SIL ISH DNP CHRC, and Incubate for [4 Minutes] > > > > 31 Red Detection [Selected] > > > > 32 Apply One Drop of RED ISH DIG MAB, Apply Coverslip, and Incubate for > [20 Minutes] > > > > 33 Apply One Drop of RED ISH DIG FR, and Incubate for [4 Minutes] > > > > 34 Counterstain [Selected] > > > > 35 Apply One Drop of [HEMATOXYLIN II] ( Counterstain ), Apply Coverslip, > and Incubate for [8 Minutes] > > > > 36 Post Counterstain [Selected] > > > > 37 Apply One Drop of [BLUING REAGENT] ( Post Counterstain ), Apply > Coverslip, and Incubate for [4 Minutes] > > > > 38 Post Run LCS Application [Selected] > > > > > > Christopher Lanigan > > Research Technologist > > Molecular Pathology > > Cleveland Clinic Foundation > > 9500 Euclid Avenue L3-127 > > Cleveland, OH 44195 > > > > > =================================== > > Please consider the environment before printing this e-mail > > Cleveland Clinic is ranked one of the top hospitals > in America by U.S.News & World Report (2010). > Visit us online at http://www.clevelandclinic.org for > a complete listing of our services, staff and > locations. > > > Confidentiality Note: This message is intended for use > only by the individual or entity to which it is addressed > and may contain information that is privileged, > confidential, and exempt from disclosure under applicable > law. If the reader of this message is not the intended > recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If > you have received this communication in error, please > contact the sender immediately and destroy the material in > its entirety, whether electronic or hard copy. Thank you. > > > ------------------------------ > > Message: 5 > Date: Thu, 9 Feb 2012 10:00:59 -0500 > From: Matt Ward > Subject: [Histonet] New Histology Positions - Northern IL - Retained > Search > To: histonet@lists.utsouthwestern.edu > Message-ID: <8b2c63aac6f85a2c063ac069a23a1e27@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Good Morning, > > > > Personify has had 2 new permanent Histology opportunities become available > in the Northern IL area. The positions offer competitive packages and the > opportunity for career growth. > > > > Please contact me directly at mw@personifysearch to learn more. > > > > *The Company:* > > Well-established provider of consumables and medical device accessories > for > clinical histology and research laboratories. The facility works closely > with our UK, German and Australian facilities in the development, > manufacture and marketing of products including processing reagents, > storage and specimen transport devices, cytology accessories and safety > products. > > This is a globally focused business with significant sales and operations > in the US, Europe and Asia Pacific as well as a direct presence in over > 100 > countries. > > *The Opportunities:* > > (1) QA Histologist: The company currently has an opening for a Quality > Assurance Histologist to be based in Richmond IL. > > (2) Histologist: The company currently has an opening for a Histologist > working on the R&D team to be based in Richmond IL. > > Salary: Based on Experience > > Other: Full benefits - 401k program/matching > > > *Education and Experience Required:* > > - Experience with tissue grossing, tissue processing and embedding > - Experience with sectioning paraffin embedded tissue as well as frozen > tissue > - Experience performing routine stains (H and E) as well as special stains > - Experience with formulation and production of routine laboratory > reagents > and solutions > - Experience performing and documenting routine laboratory procedures > - Familiarity with compliance requirements in the medical device industry > - Proficiency in basic computer skills and with software applications such > as Microsoft Office > > Have a great day! > > > > > > Matt Ward > > *Account Executive* > > *Personify* > > 5020 Weston Parkway Suite 315 > > Cary NC 27513 > > (Tel) 800.875.6188 direct ext 103 > > (Fax) 919.460.0642 > > www.personifysearch.com > > > ------------------------------ > > Message: 6 > Date: Thu, 9 Feb 2012 15:35:29 +0000 > From: Sarah Dysart > Subject: [Histonet] QIHC > To: histonet > Message-ID: > <8A70A9B2ECDD084DACFE6C59FCF86D5001CFCBCD@BL2PRD0710MB363.namprd07.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hey ya'll. I have been doing IHC for 10 years or so now. Do you think > The QIHC exam would be much of a challenge if I went and tried to do it > without much studying. I have never used automation to do IHC and have > always had to troubleshoot my by hand stains myself. If you do think > something would be worth going over what would you suggest? > Thanks > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > > ------------------------------ > > Message: 7 > Date: Thu, 9 Feb 2012 10:41:03 -0500 > From: Walter Benton > Subject: [Histonet] RE: QIHC > To: Sarah Dysart , histonet > > Message-ID: > <0B8979A204680A42B93A52B486088CD92B6A285E12@CUAEXH1.GCU-MD.local> > Content-Type: text/plain; charset="us-ascii" > > Make sure you are fimilar with ISH and IF techniques. I recall that being > part of the test. Review the criteria on the ASCP website to see what they > recommend as a study list. > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart > [sdysart@mirnarx.com] > Sent: Thursday, February 09, 2012 10:35 AM > To: histonet > Subject: [Histonet] QIHC > > Hey ya'll. I have been doing IHC for 10 years or so now. Do you think > The QIHC exam would be much of a challenge if I went and tried to do it > without much studying. I have never used automation to do IHC and have > always had to troubleshoot my by hand stains myself. If you do think > something would be worth going over what would you suggest? > Thanks > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the > intended recipient, or the employee or agent responsible for delivering it > to the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If > you have received this transmission in error, please notify the > transmitting person/department immediately by email or telephone (410) > 581-5881 and delete the message without making a copy. > > > > ------------------------------ > > Message: 8 > Date: Thu, 9 Feb 2012 15:45:19 +0000 > From: "Edwards, Richard E." > Subject: [Histonet] exit strategy for staff > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7722595275A4DD4FA225B92CDBF174A101A4F76957D8@EXC-MBX3.cfs.le.ac.uk> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have a formalised exit strategy document for when somebody > leaves an organisation, thinking about individuals either discarding or > passing on specimens etc which they have been concerned with when they > leave, and what they do with lab coats, lab books or security tags etc > when they leave, if you get my drift?, many thanks. > > Richard Edwards > > University of Leicester > > U.K. > > > > ------------------------------ > > Message: 9 > Date: Thu, 9 Feb 2012 11:16:59 -0500 > From: "Freeman, Carol" > Subject: [Histonet] (no subject) > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > Just curious how people are handling their negative control slides for > clinical settings. Thank you in advance for any responses. > > > > ------------------------------ > > Message: 10 > Date: Thu, 9 Feb 2012 09:16:43 -0800 (PST) > From: angela smith > Subject: [Histonet] Flammable / explosion proof refrigerator > To: histonet@lists.utsouthwestern.edu > Message-ID: > <1328807803.38452.YahooMailClassic@web125401.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > What is the criteria for volume and NFPA rating of a reagent for a > flammable / explosion proof refrigertor? > ? > I know many of us do not have the necessary refrigerator, but I would > assume there should be some kind of criteria or "rule" to help justify > getting an equipped refrigerator? for flammable refrigerated reagents. > ? > Please let me know some facts! > Thanks > Angela > > ------------------------------ > > Message: 11 > Date: Thu, 9 Feb 2012 09:23:03 -0800 > From: "Morken, Timothy" > Subject: RE: [Histonet] Flammable / explosion proof refrigerator > To: "'angela smith'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <8D7C2D242DBD45498006B21122072BF89F5EE7A9@MCINFRWEM003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=iso-8859-1 > > Angela, the only "requirement" I know of is your need to store solvents > that could ignite if the thermostat switch sparks. We have one for special > stains. We also have one that has one bottle of acetone in it. The vapor > from that could ignite. > > They aren't that much more expensive than a regular refrigerator. They > just put the thermostat switch on the outside rather than the inside. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela > smith > Sent: Thursday, February 09, 2012 9:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Flammable / explosion proof refrigerator > > What is the criteria for volume and NFPA rating of a reagent for a > flammable / explosion proof refrigertor? > ? > I know many of us do not have the necessary refrigerator, but I would > assume there should be some kind of criteria or "rule" to help justify > getting an equipped refrigerator? for flammable refrigerated reagents. > ? > Please let me know some facts! > Thanks > Angela > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 11 > **************************************** > From WesterM <@t> MedImmune.com Thu Feb 9 15:30:32 2012 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Thu Feb 9 15:30:38 2012 Subject: [Histonet] RE: Histonet Digest, Vol 99, Issue 11 In-Reply-To: References: Message-ID: <2FF3ED05D0191D42A22022D82494056D24942E8D@USCT1EMV002.medimmune.com> Anita- We started off with taking them down every other week and moved to once a month schedule and have had no problems, but you'd want to keep a complete defrost in mind if persistent sectioning problems ever arise. Martha ~~~~~~~~~~~~~~~~~~~~~ Martha Wester Translational Sciences MedImmune Message: 2 Date: Wed, 8 Feb 2012 16:49:29 -0600 From: anita dudley Subject: [Histonet] cryostats decontamination To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From rperez <@t> medsurgpath.com Thu Feb 9 15:34:50 2012 From: rperez <@t> medsurgpath.com (Rolly Perez) Date: Thu Feb 9 15:34:55 2012 Subject: [Histonet] Re-send Darker &/or granular hematoxylin staining (no photo) Message-ID: <9f53bfeeb2384b6c333d1333269ab744.squirrel@webmail.integra.net> Hello my colleagues, Any input will be much appreciated - what can cause darker and/or granular hematoxylin staining on the goblet cells (mucin areas) of certain GI biopsies while on other similar GIs in the very same rack of slides, they stain water clear which is the preferred outcome. I use regressive H&E staining using Sigma Aldrich Harris hemtoxylin & standard 1% Acid alcohol (hydrocholric acid). I tried stronger acid for differentiation, cut thinner, longer time in acid differentiation, less hematoxylin time, to try to get the mucin staining as minimal as possible but I can't seem to eliminate the darker &/or more granular staining on some GIs. Thanks in advance for any thoughts you might have! Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. 503.443.2157 From k84as <@t> yahoo.com Thu Feb 9 15:59:39 2012 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Thu Feb 9 15:59:47 2012 Subject: [Histonet] Re-send Darker &/or granular hematoxylin staining (no photo) In-Reply-To: <9f53bfeeb2384b6c333d1333269ab744.squirrel@webmail.integra.net> References: <9f53bfeeb2384b6c333d1333269ab744.squirrel@webmail.integra.net> Message-ID: <1328824779.36487.YahooMailNeo@web112606.mail.gq1.yahoo.com> I think these granules are of insoluble hematoxylin or preceptated hematoxylin?granules a had these condition 2 years ago and resolved it by prepairing new one. hope these could help ? Mohamed Abd el Razik Ass. Lec. of veterinary histology Cairo University- Egypt ________________________________ From: Rolly Perez To: HISTONET Sent: Thursday, February 9, 2012 11:34 PM Subject: [Histonet] Re-send Darker &/or granular hematoxylin staining (no photo) Hello my colleagues, Any input will be much appreciated - what can cause darker and/or granular hematoxylin staining on the goblet cells (mucin areas) of certain GI biopsies while on other similar GIs in the very same rack of slides, they stain water clear which is the preferred outcome. I use regressive H&E staining using Sigma Aldrich Harris hemtoxylin & standard 1% Acid alcohol (hydrocholric acid). I tried stronger acid for differentiation, cut thinner, longer time in acid differentiation, less hematoxylin time, to try to get the mucin staining as minimal as possible but I can't seem to eliminate the darker &/or more granular staining on some GIs. Thanks in advance for any thoughts you might have! Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. 503.443.2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruppert.amysue <@t> marshfieldclinic.org Thu Feb 9 19:00:58 2012 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Thu Feb 9 19:01:15 2012 Subject: [Histonet] cryostats decontamination&In-Reply-To= Message-ID: <201202100101.q1A1197v015768@mailhost3.mfldclin.edu> We use an Isopropanol based spray called DisCide Ultra Disinfecting Spray, ColePalmer catolog # EW-86306-12. Or direct from the company that makes it, www.palmerohealth.com. The spray bottle that it comes in puts out a very fine, wide spay of the disinfectant which gets it into all those hard to reach spots of a cryostat. We do this weekly along with the UV light decontamination that one of our cryotstat is equiped with. We do a more extensive/take down decontamination monthly. amysue ruppert ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From csegovia <@t> nsalabs.com Fri Feb 10 09:47:40 2012 From: csegovia <@t> nsalabs.com (Segovia, Claudia) Date: Fri Feb 10 09:45:03 2012 Subject: [Histonet] (no subject) Message-ID: <2B8BBCE0-17DC-4F9A-A8BD-AB3A2F037A37@nsalabs.com> Claudia N. Segovia Neurohistologist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csegovia@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. From POWELL_SA <@t> mercer.edu Fri Feb 10 10:36:01 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Feb 10 10:36:16 2012 Subject: [Histonet] Job in AZ Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD7D8044A@MERCERMAIL.MercerU.local> Asked to pass this on. Opportunity in AZ Full time Histotech opportunity w/Cancer Treatment Centers of America in Goodyear, AZ? This is a great opportunity offering an annual bonus, competitive pay and full (attractive) benefits. Requires Bachelor's Degree in Biological/Physical Sciences or equivalent; minimum of 2-5 yrs experience as a Histotechnologist and ASCP registration preferred. A national network of hospitals providing a comprehensive, fully integrative approach to cancer care. CTCA serves patients with advanced cancer from all 50 states at facilities located in suburban Chicago, Philadelphia, Tulsa and suburban Phoenix. CTCA offers state-of-the-art oncology treatments combined with complementary therapies to treat the disease and help improve each person's quality of life. CTCA is committed to becoming the best place to work. One way that we strive to achieve this goal is by offering a full spectrum of benefits which include: medical, dental, Healthy Awards Program, vision, flexible spending accounts, basic life and AD&D Insurance, supplemental life and AD&D insurance, short and long-term disability insurance, long-term care insurance, supplemental voluntary benefits, 401(k) savings and retirement plan, stakeholder assistance programs, adoption assistance, nutritional supplements, educational benefits, paid time off and an attractive incentive compensation program. To learn more about this opportunity and to apply online, click on the link: http://bit.ly/ypjhC8 (Histotech/Lab) Any question, you may call me directly at (651) 769-4067. Thank you. Liz Bornhofen Sr. Clinical Recruiter Cancer Treatment Centers of America (651) 769-4067 Lbornhofen@cor3talent.com From talulahgosh <@t> gmail.com Fri Feb 10 11:15:10 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 10 11:15:14 2012 Subject: [Histonet] PA area folks--company to NOT use In-Reply-To: <501018292389994543@unknownmsgid> References: <501018292389994543@unknownmsgid> Message-ID: Take it with a grain of salt, it's from a character on Parks and Rec. He's supposed to be over the top, outrageously libertarian. It's a good tv show, watch it on hulu for free. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Thu, Feb 9, 2012 at 4:20 PM, Davide Costanzo wrote: > Surely, as healthcare professionals, we can derive a better example of > American Freedom" than obesity and gluttony. > > Am I the only one offended by Mr. Swanson's quote being used in a forum > for medical professionals? Just curious. > > > Sent from my Windows Phone > From: Rene J Buesa > Sent: 2/4/2012 9:12 AM > To: histonet@lists.utsouthwestern.edu; Emily Sours > Subject: Re: [Histonet] PA area folks--company to NOT use > You should also file a complaint with the Better Business Bureau of your > area. > Ren? J. > > --- On Fri, 2/3/12, Emily Sours wrote: > > > From: Emily Sours > Subject: [Histonet] PA area folks--company to NOT use > To: histonet@lists.utsouthwestern.edu > Date: Friday, February 3, 2012, 4:12 PM > > > Hello histonetters! > > I am having a great deal of trouble getting a microscope cleaning bill > paid. We were overcharged by an hour. I suggest if you ever need your > instruments cleaned or repaired, DO NOT USE GEORGE NABLE INSTRUMENTS. He > has consistently overcharged us for his work, even after writing down a > certain price on a purchase order. > Just a warning. He works in the Pittsburgh area. > I wish there was a yelp for scientists where I could post this, because I > know a lot of people use him. > > > Emily Sours > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Feb 10 11:19:28 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Feb 10 11:19:32 2012 Subject: [Histonet] Antibody Message-ID: Looking for either IVD or ASR antibodies for MDM 2 and CDK4. Vendors ok to respond. Thank you. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From Rcartun <@t> harthosp.org Fri Feb 10 12:48:40 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Feb 10 12:48:50 2012 Subject: [Histonet] Question - Pancreatic CA Research Message-ID: <4F352038.7400.0077.1@harthosp.org> We have a patient with pancreatic cancer that is close to death. The patient has requested that her body be donated for pancreatic cancer research. Is there anyone in the Histonet Community that is working in pancreatic cancer research that can help me with this patient's request? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From srhthacker <@t> hotmail.com Fri Feb 10 12:52:19 2012 From: srhthacker <@t> hotmail.com (Stephanie Hoyle-Thacker) Date: Fri Feb 10 12:52:26 2012 Subject: [Histonet] AFB control Message-ID: Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker From liz <@t> premierlab.com Fri Feb 10 13:46:43 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Feb 10 13:46:50 2012 Subject: [Histonet] anti static spray? Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> Happy Friday Everyone I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. Thanks so much in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 From rjbuesa <@t> yahoo.com Fri Feb 10 13:54:48 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 10 13:54:53 2012 Subject: [Histonet] AFB control In-Reply-To: Message-ID: <1328903688.32287.YahooMailClassic@web162103.mail.bf1.yahoo.com> All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS Ren? J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker wrote: From: Stephanie Hoyle-Thacker Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains?? If so is there a regulation that states negative controls should be run for AFB stains.? I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 10 13:56:24 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 10 13:56:27 2012 Subject: [Histonet] anti static spray? In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> Message-ID: <1328903784.25858.YahooMailClassic@web162103.mail.bf1.yahoo.com> Probably the histology vendor is selling you the same brand product you can buy in a store, but with a different "proprietary" label. Ren? J. --- On Fri, 2/10/12, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: [Histonet] anti static spray? To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 10, 2012, 2:46 PM Happy Friday Everyone I need to purchase a product called static guard or static x? to help with static when sectioning.? Can I just purchase this from a regular store or a histology vendor?? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. Thanks so much in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Feb 10 14:05:41 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Feb 10 14:05:25 2012 Subject: [Histonet] RE: anti static spray? In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F82477@SMCMAIL01.somerset-healthcare.com> I remember buying this product years ago, before dryer sheets were invented. At the time, you could buy it in any grocery store or WalMart. What I would suggest is for you to check your humidity in the room. Quite often static is caused by low humidity levels. If possible, use a humidifier, or steamer if you have one. This should add more humidity to the room and help alleviate the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, February 10, 2012 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] anti static spray? Happy Friday Everyone I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. Thanks so much in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From hsmith <@t> wakehealth.edu Fri Feb 10 14:10:56 2012 From: hsmith <@t> wakehealth.edu (Hilary Smith) Date: Fri Feb 10 14:11:13 2012 Subject: [Histonet] Hemisecting a fresh-frozen brain Message-ID: Hi everyone I need to sagitally hemisect a fresh-frozen rhesus brain that has been stored at -80?C . Does anyone have any advice on the best way to go about this? I imagine warming it to about -20?C to reduce brittleness might be a good idea but I am not sure what my weapon of choice should be. Can't use anything that will generate heat of course. Will a non-serrated blade such as a tissue slicer blade do the trick at -20?C? Or do I need to go to something like an ultrafine wire saw? Or ultrafine hacksaw perhaps? I welcome even the most outlandish suggestions! Thanks, Hilary Hilary Smith Research Assistant Neuroimaging Lab Department of Physiology and Pharmacology Wake Forest School of Medicine Medical Center Boulevard Winston-Salem, NC 27157 Phone 336.716.8649 Fax 336.716.8689 From one_angel_secret <@t> yahoo.com Fri Feb 10 14:15:19 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Feb 10 14:15:27 2012 Subject: [Histonet] anti static spray? In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> Message-ID: <9296FAF0-979F-44B9-8965-A7B504767C23@yahoo.com> I have this problem a lot. I have static so bad literally blue sparks fly from my finger tips. I'm scared I'm going to blow up something one day and I walk around terrified to open doors. Anyway, in order to remedy my problem during sectioning I use a lot of hand lotion and get some bounce dryer sheets and run over my cloths and hair This usually works. Kim D Sent from my iPhone On Feb 10, 2012, at 2:46 PM, Elizabeth Chlipala wrote: > Happy Friday Everyone > > I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. > > Thanks so much in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Feb 10 14:16:50 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Feb 10 14:16:55 2012 Subject: [Histonet] anti static spray? In-Reply-To: <9296FAF0-979F-44B9-8965-A7B504767C23@yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC570C@SBS2K8.premierlab.local> Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Friday, February 10, 2012 1:15 PM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] anti static spray? I have this problem a lot. I have static so bad literally blue sparks fly from my finger tips. I'm scared I'm going to blow up something one day and I walk around terrified to open doors. Anyway, in order to remedy my problem during sectioning I use a lot of hand lotion and get some bounce dryer sheets and run over my cloths and hair This usually works. Kim D Sent from my iPhone On Feb 10, 2012, at 2:46 PM, Elizabeth Chlipala wrote: > Happy Friday Everyone > > I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. > > Thanks so much in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Feb 10 14:27:32 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Feb 10 14:27:39 2012 Subject: [Histonet] anti static spray? In-Reply-To: <9296FAF0-979F-44B9-8965-A7B504767C23@yahoo.com> References: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> <9296FAF0-979F-44B9-8965-A7B504767C23@yahoo.com> Message-ID: Liz, we bought anti-static mats that are grounded and placed underneath the microtomes. This helped a lot, although we do have an anti-static brush and spray that we use in extreme circumstances when the air handlers go down. I don't have the information to hand just now, but if you are ineterested I can get it to you on Monday Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, February 10, 2012 3:15 PM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] anti static spray? I have this problem a lot. I have static so bad literally blue sparks fly from my finger tips. I'm scared I'm going to blow up something one day and I walk around terrified to open doors. Anyway, in order to remedy my problem during sectioning I use a lot of hand lotion and get some bounce dryer sheets and run over my cloths and hair This usually works. Kim D Sent from my iPhone On Feb 10, 2012, at 2:46 PM, Elizabeth Chlipala wrote: > Happy Friday Everyone > > I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. > > Thanks so much in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, > LLC PO Box 18592 Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From brett_connolly <@t> merck.com Fri Feb 10 14:39:56 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Feb 10 14:40:00 2012 Subject: [Histonet] RE: Hemisecting a fresh-frozen brain In-Reply-To: References: Message-ID: Hilary, I have done this before. Definitely warm to -20C (overnight) otherwise it will likely fracture. A non-serrated brain slicing autopsy knife works well...it is best to use a knife that has a big blade and a sturdy handle. You will need a little bit of downward pressure on the knife and a slow, short sawing motion to work your way through. Cutting through the cerebral midline won't be much of a problem, be careful with the cerebellum and brainstem. Too much pressure and cutting too fast is bad. Good luck, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilary Smith Sent: Friday, February 10, 2012 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hemisecting a fresh-frozen brain Hi everyone I need to sagitally hemisect a fresh-frozen rhesus brain that has been stored at -80?C . Does anyone have any advice on the best way to go about this? I imagine warming it to about -20?C to reduce brittleness might be a good idea but I am not sure what my weapon of choice should be. Can't use anything that will generate heat of course. Will a non-serrated blade such as a tissue slicer blade do the trick at -20?C? Or do I need to go to something like an ultrafine wire saw? Or ultrafine hacksaw perhaps? I welcome even the most outlandish suggestions! Thanks, Hilary Hilary Smith Research Assistant Neuroimaging Lab Department of Physiology and Pharmacology Wake Forest School of Medicine Medical Center Boulevard Winston-Salem, NC 27157 Phone 336.716.8649 Fax 336.716.8689 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From JMacDonald <@t> mtsac.edu Fri Feb 10 14:43:03 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Feb 10 14:43:50 2012 Subject: [Histonet] AFB control In-Reply-To: <1328903688.32287.YahooMailClassic@web162103.mail.bf1.yahoo.com> Message-ID: It is recommended to run a negative AFB control to rule out false positives. Some sources of water have acid fast organisms, not TB, and if the water is used in the water baths or for staining you could pick up those organisms. The recommendation is to cut a section of tissue, known NOT to contain AFB organisms, and run it through with the positive control and patient. You would pick it up on the same water bath that you used for the patient. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 11:55 AM To histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker cc Subject Re: [Histonet] AFB control All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS Ren? J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker wrote: From: Stephanie Hoyle-Thacker Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Feb 10 14:44:55 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Feb 10 14:45:40 2012 Subject: [Histonet] anti static spray? In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> Message-ID: We also have problems with static in our student lab. We soak paper towels with water and keep them in the waste tray. Each time the student skims the water bath with a Kim wipe they also add that wet towel to the waste tray. The moisture really helps. Elizabeth Chlipala Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 11:48 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] anti static spray? Happy Friday Everyone I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. Thanks so much in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tajibade <@t> echd.org Fri Feb 10 14:45:50 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Fri Feb 10 14:46:02 2012 Subject: [Histonet] Question - Pancreatic CA Research In-Reply-To: <4F352038.7400.0077.1@harthosp.org> References: <4F352038.7400.0077.1@harthosp.org> Message-ID: Visit the website below http://www.cancer.gov/cancertopics/types/pancreatic Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, February 10, 2012 12:49 PM To: Histonet Subject: [Histonet] Question - Pancreatic CA Research We have a patient with pancreatic cancer that is close to death. The patient has requested that her body be donated for pancreatic cancer research. Is there anyone in the Histonet Community that is working in pancreatic cancer research that can help me with this patient's request? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From hsmith <@t> wakehealth.edu Fri Feb 10 14:46:56 2012 From: hsmith <@t> wakehealth.edu (Hilary Smith) Date: Fri Feb 10 14:47:07 2012 Subject: [Histonet] RE: Hemisecting a fresh-frozen brain In-Reply-To: References: Message-ID: Thanks Brett - I was hoping that someone out there had done this. If I screw up the cerebellum and brainstem it is no big deal as we are only looking at forebrain structures. This is very encouraging! Thanks again, Hilary -----Original Message----- From: Connolly, Brett M [mailto:brett_connolly@merck.com] Sent: Friday, February 10, 2012 3:40 PM To: Hilary Smith; histonet@lists.utsouthwestern.edu Subject: RE: Hemisecting a fresh-frozen brain Hilary, I have done this before. Definitely warm to -20C (overnight) otherwise it will likely fracture. A non-serrated brain slicing autopsy knife works well...it is best to use a knife that has a big blade and a sturdy handle. You will need a little bit of downward pressure on the knife and a slow, short sawing motion to work your way through. Cutting through the cerebral midline won't be much of a problem, be careful with the cerebellum and brainstem. Too much pressure and cutting too fast is bad. Good luck, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilary Smith Sent: Friday, February 10, 2012 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hemisecting a fresh-frozen brain Hi everyone I need to sagitally hemisect a fresh-frozen rhesus brain that has been stored at -80?C . Does anyone have any advice on the best way to go about this? I imagine warming it to about -20?C to reduce brittleness might be a good idea but I am not sure what my weapon of choice should be. Can't use anything that will generate heat of course. Will a non-serrated blade such as a tissue slicer blade do the trick at -20?C? Or do I need to go to something like an ultrafine wire saw? Or ultrafine hacksaw perhaps? I welcome even the most outlandish suggestions! Thanks, Hilary Hilary Smith Research Assistant Neuroimaging Lab Department of Physiology and Pharmacology Wake Forest School of Medicine Medical Center Boulevard Winston-Salem, NC 27157 Phone 336.716.8649 Fax 336.716.8689 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mpence <@t> grhs.net Fri Feb 10 14:49:04 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Feb 10 14:49:08 2012 Subject: [Histonet] AFB control In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974DBA@is-e2k3.grhs.net> Recommended by who? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, February 10, 2012 2:43 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB control It is recommended to run a negative AFB control to rule out false positives. Some sources of water have acid fast organisms, not TB, and if the water is used in the water baths or for staining you could pick up those organisms. The recommendation is to cut a section of tissue, known NOT to contain AFB organisms, and run it through with the positive control and patient. You would pick it up on the same water bath that you used for the patient. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 11:55 AM To histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker cc Subject Re: [Histonet] AFB control All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS Ren? J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker wrote: From: Stephanie Hoyle-Thacker Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Feb 10 14:52:22 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Feb 10 14:53:05 2012 Subject: [Histonet] AFB control In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974DBA@is-e2k3.grhs.net> Message-ID: Carson "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 12:49 PM To "Jennifer MacDonald" , "Rene J Buesa" cc histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker , histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] AFB control Recommended by who? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, February 10, 2012 2:43 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB control It is recommended to run a negative AFB control to rule out false positives. Some sources of water have acid fast organisms, not TB, and if the water is used in the water baths or for staining you could pick up those organisms. The recommendation is to cut a section of tissue, known NOT to contain AFB organisms, and run it through with the positive control and patient. You would pick it up on the same water bath that you used for the patient. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 11:55 AM To histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker cc Subject Re: [Histonet] AFB control All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS Ren? J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker wrote: From: Stephanie Hoyle-Thacker Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Fri Feb 10 14:56:47 2012 From: jstaruk <@t> masshistology.com (jstaruk) Date: Fri Feb 10 14:56:54 2012 Subject: [Histonet] anti static spray? In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> Message-ID: <025c01cce836$81e617c0$85b24740$@masshistology.com> We have a humidifier going most of the time. This seems to alleviate the static problems commonly experienced during the dry winter months. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, February 10, 2012 3:45 PM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] anti static spray? We also have problems with static in our student lab. We soak paper towels with water and keep them in the waste tray. Each time the student skims the water bath with a Kim wipe they also add that wet towel to the waste tray. The moisture really helps. Elizabeth Chlipala Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 11:48 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] anti static spray? Happy Friday Everyone I need to purchase a product called static guard or static x to help with static when sectioning. Can I just purchase this from a regular store or a histology vendor? Actually if anyone has any advice or suggestions on how to deal with static when sectioning that would be great. Thanks so much in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1913 / Virus Database: 2112/4801 - Release Date: 02/10/12 From DSiena <@t> statlab.com Fri Feb 10 14:57:48 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Fri Feb 10 14:57:52 2012 Subject: [Histonet] AFB control In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974DBA@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974DBA@is-e2k3.grhs.net> Message-ID: It is also a requirement if you are doing work in or for the state of New York. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, February 10, 2012 2:49 PM To: Jennifer MacDonald; Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] AFB control Recommended by who? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, February 10, 2012 2:43 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB control It is recommended to run a negative AFB control to rule out false positives. Some sources of water have acid fast organisms, not TB, and if the water is used in the water baths or for staining you could pick up those organisms. The recommendation is to cut a section of tissue, known NOT to contain AFB organisms, and run it through with the positive control and patient. You would pick it up on the same water bath that you used for the patient. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2012 11:55 AM To histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker cc Subject Re: [Histonet] AFB control All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS Ren? J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker wrote: From: Stephanie Hoyle-Thacker Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Feb 10 14:58:08 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Fri Feb 10 14:59:05 2012 Subject: [Histonet] anti static spray? References: <14E2C6176416974295479C64A11CB9AE011390CC5708@SBS2K8.premierlab.local> <9296FAF0-979F-44B9-8965-A7B504767C23@yahoo.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A7D9@UWHC-MAIL2.uwhis.hosp.wisc.edu> Are you sure it isn't just your electric personality? :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Donadio Sent: Fri 2/10/2012 2:15 PM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] anti static spray? I have this problem a lot. I have static so bad literally blue sparks fly from my finger tips. I'm scared I'm going to blow up something one day and I walk around terrified to open doors. Anyway, in order to remedy my problem during sectioning I use a lot of hand lotion and get some bounce dryer sheets and run over my cloths and hair This usually works. Kim D From gonavy2003 <@t> gmail.com Sat Feb 11 10:53:35 2012 From: gonavy2003 <@t> gmail.com (Rick Tiefenauer) Date: Sat Feb 11 10:53:40 2012 Subject: [Histonet] anti static spray Message-ID: Liz During my Navy days to help prevent static problems off X-ray films, we used a 95(ish)% isopropyl and water mix in a squeeze spray bottle. A very light mist before opening the paper film holders, and allowing it to completely evaporate, normally did the trick. I know of one histo lab here in Cali. that does a light spray of ethyl mix periodically on their blocks while chilling before microtomy, don't think they have much static issues. Watch out for over the-counter sprays from a local store, many have Butane and/or Propane (refrigerant types), and some have 1,1-Difluoroethane (normally found in computer canned air). Many have some type of fluorine-byproducts because of the high electronegativity. Cotton lab coats, and the humidity trick are also helpful. Good luck. Rick T. From thisisann <@t> aol.com Sat Feb 11 12:20:04 2012 From: thisisann <@t> aol.com (Ann Angelo) Date: Sat Feb 11 12:20:10 2012 Subject: [Histonet] Formalin training Message-ID: <8CEB700E9485795-1A90-4C7D@webmail-m165.sysops.aol.com> Does anyone have a training program/presentation that can be used to train employees about 10% NBF in the Histology lab? Ann From mfisher <@t> ecrmc.org Sat Feb 11 12:29:06 2012 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Sat Feb 11 12:29:16 2012 Subject: [Histonet] Anti Static Guns In-Reply-To: <20120211180302.B7DAD1BC04DE_F36AD56F@sophos.ecrmc.ci.el-centro.ca.us> References: <20120211180302.B7DAD1BC04DE_F36AD56F@sophos.ecrmc.ci.el-centro.ca.us> Message-ID: We use ZeroStat Antistatic Guns in our lab. Originally designed to remove fine dust particles from vinyl records (remember those?), I first began using them back in the 80's in our EM lab. When I arrived here 3 years ago and living in the desert where we have virtually no humidity, especially right now, I brought in my own personal gun (yes, I still have vinyls!) and introduced it to the lab. This gun is way over 20 years old and still works. If you google these, you will find them out there. But search around for the best price. You used to be able to walk in to a record store and buy them for about $10 but those stores are now obsolete too. I found some good prices on eBay. No chemicals, no harm to the environment, no smell, no mess! Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ? Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, February 11, 2012 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 99, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: anti static spray (Rick Tiefenauer) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Feb 2012 08:53:35 -0800 From: Rick Tiefenauer Subject: Re: [Histonet] anti static spray To: liz@premierlab.com Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Liz During my Navy days to help prevent static problems off X-ray films, we used a 95(ish)% isopropyl and water mix in a squeeze spray bottle. A very light mist before opening the paper film holders, and allowing it to completely evaporate, normally did the trick. I know of one histo lab here in Cali. that does a light spray of ethyl mix periodically on their blocks while chilling before microtomy, don't think they have much static issues. Watch out for over the-counter sprays from a local store, many have Butane and/or Propane (refrigerant types), and some have 1,1-Difluoroethane (normally found in computer canned air). Many have some type of fluorine-byproducts because of the high electronegativity. Cotton lab coats, and the humidity trick are also helpful. Good luck. Rick T. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 99, Issue 15 **************************************** ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From histotalk <@t> yahoo.com Sun Feb 12 08:29:48 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Sun Feb 12 08:29:52 2012 Subject: [Histonet] Invitation from HistoTALK Message-ID: <1329056988.63186.YahooMailNeo@web120606.mail.ne1.yahoo.com> Hello Everyone - You are all invited to join me tonight, February 12th?at 10 pm Eastern for HistoTALK www.HistoTALK.com. Tonight's guest is Lamar Jones, Technical Coordinator Pathology?from Emory University Hospital in Atlanta, GA.?HistoTALK is?a bi-monthly?"Podcast" which?is available 24/7/365. ? Yours, Dave From kdwyer3322 <@t> aol.com Sun Feb 12 20:03:45 2012 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Feb 12 20:04:01 2012 Subject: [Histonet] Texas Society for Histotechnology 2012 Convention Message-ID: <8CEB80ADA4746F3-1878-12D15@Webmail-m113.sysops.aol.com> All, The TSH meeting will be April 13-15, 2012 in San Antonio Texas at the Omni San Antonio, 9821 Colonnade Blvd. The room rate is $115.00 single/double. Room cut off date is March 22,2012 so if you plan to come to Texas please book your room as soon as possible to receive the discounted rate. The program is completed and available on our web site at txsh.org Regards, TSH Convention Committee From ragamouni.sravanthi <@t> gmail.com Mon Feb 13 05:57:22 2012 From: ragamouni.sravanthi <@t> gmail.com (ragamouni sravanthi) Date: Mon Feb 13 05:57:25 2012 Subject: [Histonet] Regarding Bone-Implant Contact Percentage Message-ID: Hai,... Can any one help me with how to calculate percentage of bone-implant contact with implants in bone sections. Please can you anyone give me detailed explanation and the required equation.Ineed it in urgency. With Regards R.Sravanthi From Dorothy.L.Webb <@t> HealthPartners.Com Mon Feb 13 09:54:18 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Feb 13 09:54:26 2012 Subject: [Histonet] formaldehyde training Message-ID: <65365F35C0F2EF4D846EC3CA73E49C430146F69CC3BF@HPEMX3.HealthPartners.int> http://www.medtraining.org There is an excellent training course and test on this website that we use for all of our lab personnel and inhouse staff that may handle formalin. It is required by OSHA to have all participants participate in some training. Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From dphillips <@t> vetmed.lsu.edu Mon Feb 13 09:53:15 2012 From: dphillips <@t> vetmed.lsu.edu (del phillips) Date: Mon Feb 13 09:55:31 2012 Subject: [Histonet] IHC and bone implants Message-ID: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> Does anyone have any suggestions for staining bone implants? I am having trouble with wash offs. I use charged slides and they are still washing off. Thanks Del From Laura.Miller <@t> leica-microsystems.com Mon Feb 13 12:05:38 2012 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Mon Feb 13 12:05:49 2012 Subject: [Histonet] AUTO: Laura Miller is Out of the Office. (returning 02/14/2012) Message-ID: I am out of the office until 02/14/2012. I am on vacation until 2-14. I will reply to your email when I return. Thank you. Note: This is an automated response to your message "Histonet Digest, Vol 99, Issue 17" sent on 2/13/2012 11:57:49 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From Jessica <@t> nsh.org Mon Feb 13 12:53:42 2012 From: Jessica <@t> nsh.org (Jessica Smith) Date: Mon Feb 13 12:53:52 2012 Subject: [Histonet] NSH One-Day VIR Forum Message-ID: <2B6E973D7D12964F8D8A3598557C5530014A33@NSH-SRVR01.nsh.local> The NSH One-Day Forum in Veterinary & Research Histology: A Focus on Immunohistochemistry is only about a month away, March 17, 2012 so get your registration in today! Come spend your St. Patrick's Day in Bethesda, MD from 8:30am-5:15pm learning IHC applications specific for veterinary diagnostic and research laboratories. Below is a schedule at a glance for the day and information on how to register! Session Topics: Session I: Antibodies Session II: IHC and Terrestrial Invertebrates Session III: Detection Systems Session IV: Double & Triple Immunostaining Session V: Trials & Tribulations of IHC Automation Session VI: Histomorphometry & Image Analysis Registration Fees Member: $139 Non Member: $159 Group Discount: Register 5 or more from your lab and receive $10 off per registration. To receive the discount, registrations must be submitted together in one fax or via mail OR online during one registration session. Register Online at: https://s3.goeshow.com/nsh/2012VIR/ereg230987.cfm?pg=home Mail or Fax: https://s3.goeshow.com/nsh/2012VIR/PDF/71199-One%20Day%20VetResearch%20Forum.pdf If you plan to fly in and stay Saturday night, Bethesda is so close to Washington, DC that you could find so many great things to do on Saturday night for St. Patrick's Day festivities! Jessica Smith Meeting Coordinator National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4060 Ext: 106 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org Visit us online for the latest news/updates! Facebook | Twitter Linked In From amber.mckenzie <@t> gastrodocs.net Mon Feb 13 13:51:58 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Feb 13 13:50:23 2012 Subject: [Histonet] QA In-Reply-To: <8CA98875B96CD09-B6C-8E4@webmail-dd20.sysops.aol.com> References: <8CA98875B96CD09-B6C-8E4@webmail-dd20.sysops.aol.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC1A57B6@JERRY.Gia.com> What % QA do most pathologists do internally? I'm trying to update my AP Manual. My last CLIA inspection, they told me to put in my AP Manual that it is done and get a binder labeled "discrepancies". If there are any... From mhale <@t> carisls.com Mon Feb 13 15:07:44 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Feb 13 15:07:59 2012 Subject: [Histonet] New Position Message-ID: <6F33D8418806044682A391273399860F0BCF8477@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From Reuel.Cornelia <@t> tsrh.org Mon Feb 13 15:33:44 2012 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Feb 13 15:34:06 2012 Subject: [Histonet] Tissue Culture No telephone regulation Message-ID: <4F392D58.077E.00C5.1@tsrh.org> Hello everyone,, I wanted to know what is the rules and regulation requirement for not having a telephone inside a tissue/Cell culture room. I have been told that we could not placed a telephone inside a culture room. But their are equipment like microscope attached to a PC, incubators or simply a call for emergency that needs access to a phone for communications. Does a telephone can create a big mess of contamination to a tissue culture room? I tried to find a policy to verify that a telephone cannot be installed inside a tissue culture room but NIH and other facility says that you just have to clean the surfaces of the phone and computer. I am not a user of the culture room but it has been so difficult to make communication when something goes wrong with the equipment inside that room since I take care of making the communication and how does the safety of the staff inside be assured if in case there is a possible fire or simply fainting inside. Please help me find this policy and I will stand to what is regulated. Thnak you very much. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From akemiat3377 <@t> yahoo.com Mon Feb 13 22:42:42 2012 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Feb 13 22:42:47 2012 Subject: [Histonet] QA In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC1A57B6@JERRY.Gia.com> References: <8CA98875B96CD09-B6C-8E4@webmail-dd20.sysops.aol.com> <5A33C952BB67F4468AF1F36D739212BC1A57B6@JERRY.Gia.com> Message-ID: 10% Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Feb 13, 2012, at 11:51 AM, Amber McKenzie wrote: > > What % QA do most pathologists do internally? I'm trying to update > my AP Manual. My last CLIA inspection, they told me to put in my > AP Manual that it is done and get a binder labeled > "discrepancies". If there are any... > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Feb 14 04:02:06 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Tue Feb 14 04:10:22 2012 Subject: [Histonet] Regarding Bone-Implant Contact Percentage In-Reply-To: References: Message-ID: Hi how are you hoping to quantitate? do you have a software programme? Our latest paper on titanium implants (in press) measure BIC expressed as a percentage of implant surface examined. On Mon, Feb 13, 2012 at 1:57 PM, ragamouni sravanthi < ragamouni.sravanthi@gmail.com> wrote: > Hai,... > Can any one help me with how to calculate percentage of bone-implant > contact with implants in bone sections. > Please can you anyone give me detailed explanation and the required > equation.Ineed it in urgency. > > > > > With Regards > R.Sravanthi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From cornettl <@t> hotmail.com Tue Feb 14 08:59:30 2012 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Tue Feb 14 08:59:38 2012 Subject: [Histonet] Re: Message-ID: My dear! http://mastercalling.com/httpnbcincome8327.php?bjkgmailID=99 Tue, 14 Feb 2012 15:59:30 _________________________________ "The Professor said:Eight! And we shall hope to follow his illustrious example." (c) Solene wera864 From trathborne <@t> somerset-healthcare.com Tue Feb 14 09:21:50 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Feb 14 09:21:38 2012 Subject: [Histonet] H. pylori Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From lblazek <@t> digestivespecialists.com Tue Feb 14 09:30:25 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 14 09:28:22 2012 Subject: [Histonet] RE: H. pylori In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA02114@IBMB7Exchange.digestivespecialists.com> I use BioCare Monoclonal Antibody with great results. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 14, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Tue Feb 14 09:29:54 2012 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Feb 14 09:30:02 2012 Subject: [Histonet] new billing Message-ID: can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama From JWeems <@t> sjha.org Tue Feb 14 09:36:09 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 14 09:36:23 2012 Subject: [Histonet] new billing In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED99F5@CHEXCMS10.one.ads.che.org> You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From TGoins <@t> mt.gov Tue Feb 14 09:47:53 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Feb 14 09:48:49 2012 Subject: [Histonet] new billing In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED99F5@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED99F5@CHEXCMS10.one.ads.che.org> Message-ID: Now I am confused. Did you mean to state "same antibody on multiple slides of the same specimen" rather than "multiple blocks of the same specimen"? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple lymph nodes are submitted from a single surgery. Thanks, Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 8:36 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Feb 14 09:55:24 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 14 09:55:38 2012 Subject: [Histonet] new billing In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED99F5@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED9A09@CHEXCMS10.one.ads.che.org> Same antibody on multiple blocks of same specimen was what I was referring to. For the past couple of years, we could charge per block. Now it is per specimen. However, it is still per block for special stains. Just to be sure that is clear... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax ________________________________ From: Goins, Tresa [mailto:TGoins@mt.gov] Sent: Tuesday, February 14, 2012 10:48 To: Weems, Joyce; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing Now I am confused. Did you mean to state "same antibody on multiple slides of the same specimen" rather than "multiple blocks of the same specimen"? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple lymph nodes are submitted from a single surgery. Thanks, Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 8:36 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From TMcNemar <@t> lmhealth.org Tue Feb 14 09:55:49 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Feb 14 09:55:39 2012 Subject: [Histonet] RE: H. pylori In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> Message-ID: Dako concentrate on the Ventana XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 14, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From alisha <@t> ka-recruiting.com Tue Feb 14 10:04:49 2012 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Feb 14 10:04:49 2012 Subject: [Histonet] New Histology Job Opportunities Message-ID: <188298309.1329235488849.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histology Job Opportunities (HT or HTL): * CA - Southern - Histotech * FL - Treasure Coast - Histotech * IN - Histotech * Maine - Histotech * NY - Westchester - Histotech (experienced) * NC - Histotech 3rd shift (experienced) * NY - Western - Histotech * NY - NYC - Histotech * NJ - Histotech * NYC - Pathology Manager (commercial background) * NYC - Histology Supervisor * NY - Long Island - Cytotech * NJ - Cytotech * NY - Westchester - Cyto Prep Technician * OH - Histotech/ Histology Supervisor * PA - Histotech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From patjnm <@t> gwumc.edu Tue Feb 14 10:18:26 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Tue Feb 14 10:18:40 2012 Subject: [Histonet] good source for APES to make silane treated slides? Message-ID: <4F3A4302.DB55.001F.1@gwumc.edu> Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From POWELL_SA <@t> mercer.edu Tue Feb 14 11:24:00 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Feb 14 11:24:13 2012 Subject: [Histonet] new billing Codes In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED9A09@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED99F5@CHEXCMS10.one.ads.che.org> <92AD9B20A6C38C4587A9FEBE3A30E1640846ED9A09@CHEXCMS10.one.ads.che.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD7E2F958@MERCERMAIL.MercerU.local> Hi Histonetters, I have a great idea. You can come to Georgia for our meeting, our own Joyce Weems will be presenting a workshop on CPT coding at our meeting. The Georgia Society for Histotechnology - Region III meeting will be held at Callaway Gardens, Pine Mountain GA April 13-15th,. The Deadline for receiving the discounted hotel rate is March 13th, so act now. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here in Georgia. Making reservations with a credit card will hold your room, they will not apply the rate until you actually check in. Call now 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program which can be found at http://www.histosearch.com/gsh/symposium.html. Also register early for the fantastic workshops GSH has lined up, like Joyce's and earn your CEUs. GSH has planned a great awards luncheon on Saturday as well as a dinner on Saturday night, details are in the program. Payment is easy with PayPal. You do not have to have an account with them to use this service and it is secure. Make payment by going to www.PayPal.com and send payment to the email address on the registration form. Plan to attend, bring the family for a vacation, Callaway Gardens is a wonderful family experience. If you have any questions please contact GSH President - Mike Ayers at lmayers@charter.net, GSH Vice President and Exhibit Liaison - Wanda Simons at wandrous@att.net or myself powell_sa@mercer.edu. Please pass this invitation to any who may not get this email. Come to Georgia and experience Histotechnology - Southern Style -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 10:55 AM To: Goins, Tresa; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing Same antibody on multiple blocks of same specimen was what I was referring to. For the past couple of years, we could charge per block. Now it is per specimen. However, it is still per block for special stains. Just to be sure that is clear... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax ________________________________ From: Goins, Tresa [mailto:TGoins@mt.gov] Sent: Tuesday, February 14, 2012 10:48 To: Weems, Joyce; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing Now I am confused. Did you mean to state "same antibody on multiple slides of the same specimen" rather than "multiple blocks of the same specimen"? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple lymph nodes are submitted from a single surgery. Thanks, Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 8:36 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama From andreahooper <@t> rocketmail.com Tue Feb 14 11:49:21 2012 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Tue Feb 14 11:49:31 2012 Subject: [Histonet] Hight throughput IHC Message-ID: <4B6C60E6-7AF0-4845-A069-C92AF4597A30@rocketmail.com> Is anyone doing any type of high throughput IHC testing? If so I would love to pick your brain. Andrea T. Hooper, PhD andreahooper@rocketmail.com From mcauliff <@t> umdnj.edu Tue Feb 14 12:44:55 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Feb 14 12:43:49 2012 Subject: [Histonet] good source for APES to make silane treated slides? In-Reply-To: <4F3A4302.DB55.001F.1@gwumc.edu> References: <4F3A4302.DB55.001F.1@gwumc.edu> Message-ID: <4F3AABA7.40709@umdnj.edu> Sigma Chemical, catalog number A3648 Geoff On 2/14/2012 11:18 AM, Joseph Madary wrote: > > > > Nick Madary, HT/HTL(ASCP)QIHC > George Washington University > Pathology Core Laboratory > Ross Hall, Room 706 > 23rd and I Street NW > Washington D.C. 20037 > 202.994.8916 > patjnm@gwumc.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From w_alkadhumi <@t> yahoo.com Tue Feb 14 12:44:33 2012 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Tue Feb 14 12:45:41 2012 Subject: [Histonet] wrinkles Message-ID: <1329245073.26066.YahooMailNeo@web45203.mail.sp1.yahoo.com> Dear histonet members First?I wish every one?a happy valentine day!!! At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When?I trim the paraffin embedded tissue my sections?are faultless?.?Now because it is winter I have a problem having wrinkles in the sectionsafter collecting them from the water bath. Does anyone recommend me?to buy?slide drying hotplate for the slides?especially for the IHC slides, if you recommend me to buy it at what temperature should I use it and how it would effect the?baking time afterward? ? Thanks Wassan/Histotechnican Shorsh General?Hospital North of Iraq From CIngles <@t> uwhealth.org Tue Feb 14 13:22:16 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Feb 14 13:22:20 2012 Subject: [Histonet] (no subject) References: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> <5A2BD13465E061429D6455C8D6B40E39137DA02114@IBMB7Exchange.digestivespecialists.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A7DC@UWHC-MAIL2.uwhis.hosp.wisc.edu> Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active "enzymes" in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. Claire From Rcartun <@t> harthosp.org Tue Feb 14 13:48:38 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Feb 14 13:48:51 2012 Subject: [Histonet] H. pylori In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> Message-ID: <4F3A7445.7400.0077.1@harthosp.org> Keep in mind that many of the commercially-available monoclonal antibodies to H. pylori do not label Helicobacter helilmannii, often seen in children, especially those with exposure to animals. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 2/14/2012 10:21 AM >>> Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Feb 14 14:36:39 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Feb 14 14:36:43 2012 Subject: [Histonet] IHC and bone implants In-Reply-To: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> References: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> Message-ID: Del, I have a few questions for you to better understand your problem. 1) Are you talking about decalcified paraffin sections or undecalcified resin embedded specimens? 2) Is the implant washing off or both the bone and implant? 3) What type of implant/material is it? 4) If decalcified and paraffin embedded, what temperature and how long do you melt your specimens to the slide on the slide warmer? 5) If undecalcified and resin embedded, what type of resin and how thick are your sections? 6) Lastly, what step do you notice that the section or implant becomes detached from the slide? Please forgive me for the list of questions, but a little more information will help to pinpoint the problem and help get straight to the best corrective action. Best Regards, Jack Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology Senior Histologist, Biomimetic Therapeutics, Inc. > From: dphillips@vetmed.lsu.edu > To: Histonet@lists.utsouthwestern.edu > Date: Mon, 13 Feb 2012 09:53:15 -0600 > CC: > Subject: [Histonet] IHC and bone implants > > Does anyone have any suggestions for staining bone implants? I am having > trouble with wash offs. I use charged slides and they are still washing off. > > > > Thanks > > Del > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 14 14:55:30 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 14 14:55:34 2012 Subject: [Histonet] (no subject) In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A7DC@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <1329252930.27538.YahooMailClassic@web162102.mail.bf1.yahoo.com> About not using diastase for PASD because "there are active enzymes in the tissue": that is wrong because those are the same enzymes active in any tissue just before it is fixed. Also remember that you are going to fix with EthOL. The whole issue behind the PASD (as you well know) is to compare the results of the PAS with and without diastase, so your co-worker is wrong. As to not heating the silver for GMS I do see any reason to not doing that. If you do not heat it you will get something different to GMS. Perhaps your co-worker is fearful that the section may peel-off because of the heat but, other than that, there is no reason. Ren? J. ? ? ? ? --- On Tue, 2/14/12, Ingles Claire wrote: From: Ingles Claire Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 14, 2012, 2:22 PM Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active "enzymes" in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 14 14:58:24 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 14 14:58:27 2012 Subject: [Histonet] wrinkles In-Reply-To: <1329245073.26066.YahooMailNeo@web45203.mail.sp1.yahoo.com> Message-ID: <1329253104.25837.YahooMailClassic@web162102.mail.bf1.yahoo.com> If you consider that it\\your lab?is cold now in winter, have you checked out the temperature in the water bath? Perhaps it is too cold to allow the sections to expand. If the water bath keeps its temperature (about 45?C) the room temperature is irrelevant. Ren? J. --- On Tue, 2/14/12, wassan alkadhumi wrote: From: wassan alkadhumi Subject: [Histonet] wrinkles To: "h n" Date: Tuesday, February 14, 2012, 1:44 PM Dear histonet members First?I wish every one?a happy valentine day!!! At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When?I trim the paraffin embedded tissue my sections?are faultless?.?Now because it is winter I have a problem having wrinkles in the sectionsafter collecting them from the water bath. Does anyone recommend me?to buy?slide drying hotplate for the slides?especially for the IHC slides, if you recommend me to buy it at what temperature should I use it and how it would effect the?baking time afterward? ? Thanks Wassan/Histotechnican Shorsh General?Hospital North of Iraq _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Feb 14 15:33:36 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Feb 14 15:33:39 2012 Subject: [Histonet] QA by pathologists Message-ID: Akemi Allison asks: >>What % QA do most pathologists do internally? I'm trying to update my AP Manual. My last CLIA inspection, they told me to put in my AP Manual that it is done and get a binder labeled "discrepancies". If there are any...<< As far as I know, there is no percentage requirement like the burdensome and useless 10% rescreen on Pap smears. In my travels I've seen: One three-pathologist group did 100% review before sign-out. At least two services I've seen did a 10% review, cases chosen at random. This seemed really pointless. One service did a 10% review, with the originating pathologist choosing the cases to be reviewed. This fairly productive. One five-pathologist service meticulously documented informal consultation, both in the report and on a separate piece of paper. They aimed at, and got, a 2.8% review. In a multiple pathologist practice, I think this was the most productive method I ever saw. Bob Richmond Samurai Pathologist Knoxville TN From Vickroy.Jim <@t> mhsil.com Tue Feb 14 16:31:29 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Feb 14 16:31:28 2012 Subject: [Histonet] New DNA Probes for Kappa and Lambda Message-ID: <24A4826E8EF0964D86BC5317306F58A55FDC5DE697@mmc-mail.ad.mhsil.com> In the past we have used a cocktail DNA probe (ISH) for Kappa and Lambda. Has anybody had any experience validating and setting up the new probes from Ventana which we have to mix the four probes together and form our own cocktail? Please share with me your thoughts. I am seriously considering sending this test out because of the infrequency, time, ASR hassle, costs of separate detection kits, etc. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From tony.henwood <@t> health.nsw.gov.au Tue Feb 14 17:04:54 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Feb 14 17:05:08 2012 Subject: [Histonet] (no subject) In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A7DC@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <3AD061FE740D464FAC7BF6B5CFB7570711F829FF@SMCMAIL01.somerset-healthcare.com> <5A2BD13465E061429D6455C8D6B40E39137DA02114@IBMB7Exchange.digestivespecialists.com> <064F1ACBAE8A78469AE2E41D533D87E505A7DC@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A1AAFF@xmdb02.nch.kids> The reason for using diastase is to remove the glycogen from the tissues. AND if you do not heat the methenamine silver solution for the GMS it will take forever to stain, in fact all you will get is background spots. This I can verify, have you ever forgot to turn on the water bath before staining? - several hours later - nuthin!! Who ever is giving you the advice I would wonder about their histotechnology education and training (in fact have they had any training at all?). Do not listen to them. Extrapolate from cytology. GMS and DiPAS have been done on cytology smears fixed in ethanol as well as air-dried for years see: Loughman NT. "Pneumocystis carinii: rapid diagnosis with the microwave oven" Acta Cytologica. 33(3):416-7, 1989 May-Jun. PINTOZZI, R. L. "Modified Grocott's methenamine silver nitrate method for quick staining of Pneumocystis carinii" J Clin Pathol 1978 31: 803-805 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, 15 February 2012 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active "enzymes" in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Marilyn.Tyler <@t> uct.ac.za Wed Feb 15 00:14:10 2012 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Wed Feb 15 00:14:24 2012 Subject: [Histonet] endogeneous peroxidase blocking Message-ID: <4F3B6952020000900012F35B@gwiasmtp.uct.ac.za> Morning All Posing this question for a colleague. Suggestions for immunohistochemistry blocking for endogeneous peroxidase probably caused by red blood cells in mouse lung tissue. Using 1% hydrogen peroxide/dist.water for 15mins and still getting non specific staining. Thank you Marilyn Medical School UCT Dept of Surgery J50/26 or 30 Surgical research. OMB Groote Schuur Hospital Observatory 021-4066227 ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From equineshowmom01 <@t> yahoo.com Wed Feb 15 03:27:14 2012 From: equineshowmom01 <@t> yahoo.com (Sheree H) Date: Wed Feb 15 03:27:22 2012 Subject: [Histonet] (no subject) Message-ID: <1329298034.80174.YahooMailMobile@web125806.mail.ne1.yahoo.com> http://www.kingtigertkdwilson.com/modules/mod_wdbanners/myfriends.php?central164.gif From sadey <@t> hotmail.ca Wed Feb 15 04:52:19 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Wed Feb 15 04:52:28 2012 Subject: [Histonet] Meditech users Message-ID: Hello:Are any of the Meditech users having the pathologists order their levels in the computer?We would like to implement this procedure. Any suggestions. :)ThanksSheila From estellamireles <@t> gmail.com Wed Feb 15 09:43:50 2012 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Wed Feb 15 09:43:56 2012 Subject: [Histonet] Independent Service Technician Message-ID: Is there anyone out there that does repairs on Sakura equipment and works for themselves in the Texas area. From dreynold <@t> mdanderson.org Wed Feb 15 10:49:27 2012 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Wed Feb 15 10:49:35 2012 Subject: [Histonet] Re: bone implants In-Reply-To: References: Message-ID: <785BBF0C5F49CE41BA74460A43A08F023055EE4325@DCPWVMBXC0VS3.mdanderson.edu> I don't know about implants but we were having a terrible time with bone marrows coming off. Usually they came off during the HEIR using the steamer. At that point we were placing our slides lying flat in a 55-60? oven overnight which usually helps tremendously with difficult tissue. This was followed with routine deparaffinization and hydration. It was suggested to us to try a 70? water bath for 2-4 hours for HEIR. I was doubtful that my antibodies would work but they did. In fact the staining was better with 2 hr HEIR than with 4. Donna Reynolds Chief Histology laboratory, Research Department Cancer Biology U.T. M.D. Anderson Cancer Center, Houston, TX. 713-792-8106 Message: 4 Date: Mon, 13 Feb 2012 09:53:15 -0600 From: "del phillips" Subject: [Histonet] IHC and bone implants To: Message-ID: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> Content-Type: text/plain; charset="us-ascii" Does anyone have any suggestions for staining bone implants? I am having trouble with wash offs. I use charged slides and they are still washing off. Thanks Del From rsrichmond <@t> gmail.com Wed Feb 15 12:30:11 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 15 12:30:27 2012 Subject: [Histonet] Meditech users - pathologists ordering levels Message-ID: Sheila asks: >>Hello: Are any of the Meditech users having the pathologists order their levels in the computer? We would like to implement this procedure. Any suggestions?<< Never seen this - but it sounds like a nifty little time-waster for all concerned. I usually examine the paraffin block before ordering levels or other recuts - to make sure the block isn't exhausted or horrible. Am I the only pathologist who does this? Bob Richmond Samurai Pathologist Knoxville TN From Sarah_Mack <@t> urmc.rochester.edu Wed Feb 15 12:31:33 2012 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Wed Feb 15 12:32:10 2012 Subject: [Histonet] Region 1 Symposium Message-ID: Region 1 Symposium Announcement The New York State Histotechnological Society is hosting the 2012 Region 1 Histotechnologist Symposium to be held at the Marriott Islandia Long Island on April 27th and 28th 2012. The program/registration, exhibitor and hotel/travel information is available on the NYSHS website at: http://www.nyhisto.org/meetings/current-nys-meeting/ We look forward to seeing you there! From pruegg <@t> ihctech.net Wed Feb 15 12:39:33 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 15 12:39:39 2012 Subject: [Histonet] Re: bone implants In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F023055EE4325@DCPWVMBXC0VS3.mdanderson.edu> References: <785BBF0C5F49CE41BA74460A43A08F023055EE4325@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: <07FEB4157B3A49BC809C8E58B36457C3@Patsyoffice> This is an age old problem, bone comes off easily especially during HIER, which is why I have adapted all my bone IHC abs to use EIER instead of HIER, usually with pepsin or proteinase K. Also, it helps to heat the slides flat on a slide warmer at 45dc overnight. If all else fails I use elmer glue coated slides but those can be problematic because the glue may react with some ab reagents. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Wednesday, February 15, 2012 9:49 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: bone implants I don't know about implants but we were having a terrible time with bone marrows coming off. Usually they came off during the HEIR using the steamer. At that point we were placing our slides lying flat in a 55-60? oven overnight which usually helps tremendously with difficult tissue. This was followed with routine deparaffinization and hydration. It was suggested to us to try a 70? water bath for 2-4 hours for HEIR. I was doubtful that my antibodies would work but they did. In fact the staining was better with 2 hr HEIR than with 4. Donna Reynolds Chief Histology laboratory, Research Department Cancer Biology U.T. M.D. Anderson Cancer Center, Houston, TX. 713-792-8106 Message: 4 Date: Mon, 13 Feb 2012 09:53:15 -0600 From: "del phillips" Subject: [Histonet] IHC and bone implants To: Message-ID: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> Content-Type: text/plain; charset="us-ascii" Does anyone have any suggestions for staining bone implants? I am having trouble with wash offs. I use charged slides and they are still washing off. Thanks Del _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lanigac <@t> ccf.org Wed Feb 15 13:13:26 2012 From: lanigac <@t> ccf.org (Lanigan, Christopher) Date: Wed Feb 15 13:13:50 2012 Subject: [Histonet] New DNA Probes for Kappa and Lambda Message-ID: <9DDD0F026DC71B41B79D8E439D7951A30BAB47AE@cchsclexmb69.cc.ad.cchs.net> ------------ Original Message -------------- Message: 9 Date: Tue, 14 Feb 2012 16:31:29 -0600 From: "Vickroy, Jim" Subject: [Histonet] New DNA Probes for Kappa and Lambda To: "histonet@lists.utsouthwestern.edu" Message-ID: <24A4826E8EF0964D86BC5317306F58A55FDC5DE697@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" In the past we have used a cocktail DNA probe (ISH) for Kappa and Lambda. Has anybody had any experience validating and setting up the new probes from Ventana which we have to mix the four probes together and form our own cocktail? Please share with me your thoughts. I am seriously considering sending this test out because of the infrequency, time, ASR hassle, costs of separate detection kits, etc. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ------------ Reply -------------- Hi Jim Yes, I have successful Kappa / Lambda ISH protocol for the Ventana Benchmark XT and also for the Ventana Benchmark ULTRA. First, the software that you will need to have installed is called either "XT ISH Open Probes ChromogenicV3" & "U ISH Open Probes ChromogenicV3" - depending whether you are running the Benchmark XT or ULTRA respectively. These protocols will use the iView BLUE PLUS DETECTION and with a RED STAIN II counterstain. I believe each protocol takes between 6 to 7 hours. Before I get to the protocols, I'll fill you in on the Kappa and Lambda probes. As you know, they do not arrive in a dispenser. They each arrive in 4 pre-diluted vials, so you will need to fill a "user-fillable dispenser". All four pre-diluted vials are mixed together into one user-fillable dispenser to make the "cocktail". Apparently, Ventana is required to package each separately. One more thing, you will need to run a control. This control will confirm the presence of non-degradated RNA. The control I used was U6, and the protocol is identical to the Kappa or Lambda except for the selected ISH probe. Therefore, you need 3 slides per case. By the way, the selected ISH probe will likely be "ISH PROBE 1", "ISH PROBE 2", etc - because the user-fillable dispenser will not have the proper commercial bar code. Finally, the following are successful protocol summaries. The first is for the Ventana Benchmark XT, and the second is for the Ventana Benchmark ULTRA. Benchmark XT: 1 Baking [Selected] 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking ) 3 Deparaffinization [Selected] 4 Standard [Selected] 5 Warmup Slide to [69 Deg C], and Incubate for 4 Minutes ( Deparaffinization ) 6 Enzyme [Selected] 7 Apply Coverslip, One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate for [8 Minutes] 8 Probe [Selected] 9 Probe Auto Dispense [Selected] 10 1 Drop of Probe Dispensed [Selected] 11 Apply One Drop of [ISH Probe 1] ( ISH Probe ), Apply Coverslip, and Incubate for 4 Minutes 12 Denature [Selected] 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( Denaturation ) 14 Hybridization [Selected] 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 16 Incubate for [1 Hour] ( Hybridization ) 17 Stringency Washes [Selected] 18 Stringency Wash #1 [Selected] 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 20 Stringency Wash #2 [Selected] 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) 22 Stringency Wash #3 [Selected] 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) 24 Detection Kit [Selected] 25 Blue Detection [Selected] 26 Incubate for [32 Minutes] ( Substrate ) 27 Counterstain [Selected] 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 29 Post Run LCS Application [Selected] Benchmark Ultra: 1 Baking [Selected] 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking ) 3 Deparaffinization [Selected] 4 Standard [Selected] 5 Warmup Slide to [69 Deg C] from High Temperatures ( Deparaffinization ) 6 Enzyme [Selected] 7 Apply One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate for [8 Minutes] 8 Probe [Selected] 9 Probe Auto Dispense [Selected] 10 1 Drop of Probe Dispensed [Selected] 11 Apply One Drop of [ISH Probe 2] ( ISH Probe ), No Coverslip and Incubate for 4 Minutes 12 Denature [Selected] 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( Denaturation ) 14 Hybridization [Selected] 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 16 Incubate for [1 Hour] ( Hybridization ) 17 Stringency Washes [Selected] 18 Stringency Wash #1 [Selected] 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 20 Stringency Wash #2 [Selected] 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) 22 Stringency Wash #3 [Selected] 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) 24 Detection Kit [Selected] 25 Blue Detection [Selected] 26 Incubate for [32 Minutes] ( Substrate ) 27 Counterstain [Selected] 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 29 Post Run LCS Application [Selected] Chris Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News & World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From Kim.Kolman <@t> va.gov Wed Feb 15 14:26:25 2012 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Wed Feb 15 14:26:53 2012 Subject: [Histonet] alcohol hydrometer Message-ID: <9C32F30B6662D74A8419DDDB7E66656A06D13018@VHAV15MSGA1.v15.med.va.gov> Can anyone tell me where I could find a small (6-7 inches long) alcohol hydrometer? Needs to read to 200% proof. All I'm finding is a 12" size and I would like to be able to test in a 100ml cylinder. Thanks for your help, Kim Kimberly D. Kolman, HT, (ASCP) Diagnostics 115 VA Eastern Kansas Health Care System 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 ph: 913-682-2000 x 52537/52539 From kim.tournear <@t> yahoo.com Wed Feb 15 14:36:11 2012 From: kim.tournear <@t> yahoo.com (Yahoo) Date: Wed Feb 15 14:36:25 2012 Subject: [Histonet] alcohol hydrometer In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A06D13018@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A06D13018@VHAV15MSGA1.v15.med.va.gov> Message-ID: <4348D61A-06F4-41CA-8C26-83DD77AE5ADB@yahoo.com> I've never seen one that small. Sent from the iPhone of Kim Tournear On Feb 15, 2012, at 2:26 PM, "Kolman, Kimberly D." wrote: > Can anyone tell me where I could find a small (6-7 inches long) alcohol > hydrometer? Needs to read to 200% proof. > > All I'm finding is a 12" size and I would like to be able to test in a > 100ml cylinder. > > > > Thanks for your help, > > Kim > > > > Kimberly D. Kolman, HT, (ASCP) > > Diagnostics 115 > > VA Eastern Kansas Health Care System > > 4101 S. 4th St. Trfwy. > > Leavenworth, KS 66048 > > ph: 913-682-2000 x 52537/52539 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From collette2 <@t> llnl.gov Wed Feb 15 14:38:47 2012 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Wed Feb 15 14:39:04 2012 Subject: [Histonet] RNAlater regret Message-ID: Hello, Esteemed Histonetters! I have a collaborator who asked me to drop his (mouse) bones into RNAlater for RNA isolation, later. These animals were double labeled for histomorphometry as well. He now wants to know if he can back them out of RNAlater and use them for any histology-related protocols? (dynamic histomorphometry, histology stains, immunostains)? Perhaps the best course of action is to back them into fixative and proceed with fingers crossed? If anyone has a better idea or already knows it won't work, it would be much appreciated. Thanks, and Happy Wednesday, Sincerely, Nicole Collette LLNL From daniela.bodemer <@t> mcri.edu.au Wed Feb 15 16:59:05 2012 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Wed Feb 15 16:59:16 2012 Subject: [Histonet] Oil Red O Message-ID: <9DF797D618351549B984596F01A1FE1D0231FC21@murmx.mcri.edu.au> Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading and should they be kept in a folder in the fridge? Thanks for your thoughts, Daniela Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From rosenfeldtek <@t> hotmail.com Wed Feb 15 17:53:00 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Wed Feb 15 17:53:04 2012 Subject: [Histonet] Ribbon won't stay on knife Message-ID: Sometimes while cutting 5 micron paraffin sections... I turn the wheel once and a nice section gets cut and lays down on my blade. When I turn the wheel again, as the knife holder is traveling Upwards, the top of my paraffin block will grab the ribbon and lift it off of the microtome blade, ruining my ribbon. Any ideas what is going on, how to fix it. Today I'm using my old trust Spencer 820 microtome, but it also sometimes happens on my Leica RM2125. Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology From rosenfeldtek <@t> hotmail.com Wed Feb 15 18:36:16 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Wed Feb 15 18:36:27 2012 Subject: [Histonet] Ribbon won't stay on knife. Why didnt I think of that... In-Reply-To: References: , Message-ID: Checking the troubleshooting manual for the Leica 2525... Problem: "Specimen is picked up in the return stroke of the specimen arm." Possible Cause: "Static electricity charge may be built up on the knife holder or specimen head." Corrective Action: Clean components of microtome with alcohol (one of 3 suggestions.) That one worked. Jerry From: lynnd01@hotmail.com To: rosenfeldtek@hotmail.com Subject: RE: [Histonet] Ribbon won't stay on knife Date: Wed, 15 Feb 2012 18:25:34 -0600 A couple of things to look at...check your angle...not all knife holders are the same. Is your blade sharp? Is the block cold and is the bottom of the block parallel to the knife? Sometimes I'll take my thumbnail and run it across the bottom edge of the block..but be careful not to cut your thumb. Lynn > From: rosenfeldtek@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 15 Feb 2012 15:53:00 -0800 > Subject: [Histonet] Ribbon won't stay on knife > > > Sometimes while cutting 5 micron paraffin sections... > > I turn the wheel once and a nice section gets cut and lays down on my blade. When I turn the wheel again, as the knife holder is traveling Upwards, the top of my paraffin block will grab the ribbon and lift it off of the microtome blade, ruining my ribbon. Any ideas what is going on, how to fix it. > > Today I'm using my old trust Spencer 820 microtome, but it also sometimes happens on my Leica RM2125. > > Thanks, > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srhthacker <@t> hotmail.com Wed Feb 15 23:03:35 2012 From: srhthacker <@t> hotmail.com (Stephanie Hoyle-Thacker) Date: Wed Feb 15 23:03:43 2012 Subject: [Histonet] Re: Message-ID: ...Seems like you should give this a try http://audacesidea.com.br/day.link.php?ykegoogleId=88or8 From madeleinehuey <@t> gmail.com Wed Feb 15 23:04:34 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Wed Feb 15 23:04:45 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 19 In-Reply-To: <4f3bf2fb.1584650a.2d68.ffffda15SMTPIN_ADDED@mx.google.com> References: <4f3bf2fb.1584650a.2d68.ffffda15SMTPIN_ADDED@mx.google.com> Message-ID: From: "del phillips" Subject: [Histonet] IHC and bone implants Does anyone have any suggestions for staining bone implants? I am having trouble with wash offs. I use charged slides and they are still washing off. Thanks Del Del, Since you did not mention what AR buffer & time for your IHC and here's my suggestion; 1) HIER with Diva (Biocare) in a Pascal (or similar) pressure cooker for 3 min 2) Rinse slide immediately after pressure cooker is done (NO cooling in room temperature for any length of time) 3) Proceed your IHC protocol Note: If you still have problem after following the above instruction, then try Fisher Scientific "Ultra Stick slides - Cat. # ???". It's relatively same price as regular charge slides, cheaper than Dako IHC slides. In the past, I have done IHC/IFC with 100 um Frozen section with cheap pressure cooker & the thick section still stick to the slide with excellent morphology. Good Luck! Madeleine Huey BS, HTL/QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Wed, Feb 15, 2012 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Re: good source for APES to make silane treated slides? > ? ? ?(Geoff McAuliffe) > ? 2. wrinkles (wassan alkadhumi) > ? 3. (no subject) (Ingles Claire ) > ? 4. Re: H. pylori (Richard Cartun) > ? 5. RE: IHC and bone implants (Jack Ratliff) > ? 6. Re: (no subject) (Rene J Buesa) > ? 7. Re: wrinkles (Rene J Buesa) > ? 8. QA by pathologists (Bob Richmond) > ? 9. New DNA Probes for Kappa and Lambda (Vickroy, Jim) > ?10. RE: (no subject) (Tony Henwood (SCHN)) > ?11. endogeneous peroxidase blocking (Marilyn Tyler) > ?12. (no subject) (Sheree H) > ?13. Meditech users (Sheila Adey) > ?14. Independent Service Technician (Stella Mireles) > ?15. Re: bone implants (Reynolds,Donna M) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 14 Feb 2012 13:44:55 -0500 > From: Geoff McAuliffe > Subject: Re: [Histonet] good source for APES to make silane treated > ? ? ? ?slides? > To: histonet@lists.utsouthwestern.edu > Message-ID: <4F3AABA7.40709@umdnj.edu> > Content-Type: text/plain; CHARSET=US-ASCII; format=flowed > > Sigma Chemical, catalog number A3648 > > Geoff > > > On 2/14/2012 11:18 AM, Joseph Madary wrote: >> >> >> >> Nick Madary, HT/HTL(ASCP)QIHC >> George Washington University >> Pathology Core Laboratory >> Ross Hall, Room 706 >> 23rd and I Street NW >> Washington D.C. 20037 >> 202.994.8916 >> patjnm@gwumc.edu >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > ------------------------------ > > Message: 2 > Date: Tue, 14 Feb 2012 10:44:33 -0800 (PST) > From: wassan alkadhumi > Subject: [Histonet] wrinkles > To: h n > Message-ID: > ? ? ? ?<1329245073.26066.YahooMailNeo@web45203.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear histonet members > First?I wish every one?a happy valentine day!!! > At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When?I trim the paraffin embedded tissue my sections?are faultless?.?Now because it is winter I have a problem having wrinkles in the sectionsafter collecting them from the water bath. Does anyone recommend me?to buy?slide drying hotplate for the slides?especially for the IHC slides, if you recommend me to buy it at what temperature should I use it and how it would effect the?baking time afterward? > > Thanks > Wassan/Histotechnican > Shorsh General?Hospital > North of Iraq > > ------------------------------ > > Message: 3 > Date: Tue, 14 Feb 2012 13:22:16 -0600 > From: "Ingles Claire " > Subject: [Histonet] (no subject) > To: > Message-ID: > ? ? ? ?<064F1ACBAE8A78469AE2E41D533D87E505A7DC@UWHC-MAIL2.uwhis.hosp.wisc.edu> > > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > Hey histo gurus: > Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active "enzymes" in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. > Claire > > > > ------------------------------ > > Message: 4 > Date: Tue, 14 Feb 2012 14:48:38 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] H. pylori > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?"Toni Rathborne" > ? ? ? ? > Message-ID: <4F3A7445.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Keep in mind that many of the commercially-available monoclonal antibodies to H. pylori do not label Helicobacter helilmannii, often seen in children, especially those with exposure to animals. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > >>>> "Rathborne, Toni" 2/14/2012 10:21 AM >>> > Happy Valentines Day! > > Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. > As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) > > Toni > > > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. ?The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. ?Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. ?If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Tue, 14 Feb 2012 15:36:39 -0500 > From: Jack Ratliff > Subject: RE: [Histonet] IHC and bone implants > To: , Histonet > ? ? ? ? > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Del, > > I have a few questions for you to better understand your problem. > > 1) ?Are you talking about decalcified paraffin sections or undecalcified resin embedded specimens? > > 2) ?Is the implant washing off or both the bone and implant? > > 3) ?What type of implant/material is it? > > 4) ?If decalcified and paraffin embedded, what temperature and how long do you melt your specimens to the slide on the slide warmer? > > 5) ?If undecalcified and resin embedded, what type of resin and how thick are your sections? > > 6) ?Lastly, what step do you notice that the section or implant becomes detached from the slide? > > Please forgive me for the list of questions, but a little more information will help to pinpoint the problem and help get straight to the best corrective action. > > Best Regards, > > Jack > > > Jack Ratliff > Chairman, Hard Tissue Committee - National Society for Histotechnology > Senior Histologist, Biomimetic Therapeutics, Inc. > > > > >> From: dphillips@vetmed.lsu.edu >> To: Histonet@lists.utsouthwestern.edu >> Date: Mon, 13 Feb 2012 09:53:15 -0600 >> CC: >> Subject: [Histonet] IHC and bone implants >> >> Does anyone have any suggestions for staining bone implants? I am having >> trouble with wash offs. I use charged slides and they are still washing off. >> >> >> >> Thanks >> >> Del >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 6 > Date: Tue, 14 Feb 2012 12:55:30 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu, Ingles Claire > ? ? ? ? > Message-ID: > ? ? ? ?<1329252930.27538.YahooMailClassic@web162102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > About not using diastase for PASD because "there are active enzymes in the tissue": that is wrong because those are the same enzymes active in any tissue just before it is fixed. Also remember that you are going to fix with EthOL. The whole issue behind the PASD (as you well know) is to compare the results of the PAS with and without diastase, so your co-worker is wrong. > As to not heating the silver for GMS I do see any reason to not doing that. If you do not heat it you will get something different to GMS. Perhaps your co-worker is fearful that the section may peel-off because of the heat but, other than that, there is no reason. > Ren? J. > > > > > --- On Tue, 2/14/12, Ingles Claire wrote: > > > From: Ingles Claire > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 14, 2012, 2:22 PM > > > Hey histo gurus: > Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active "enzymes" in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. > Claire > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 7 > Date: Tue, 14 Feb 2012 12:58:24 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] wrinkles > To: h n , ? ?wassan alkadhumi > ? ? ? ? > Message-ID: > ? ? ? ?<1329253104.25837.YahooMailClassic@web162102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If you consider that it\\your lab?is cold now in winter, have you checked out the temperature in the water bath? Perhaps it is too cold to allow the sections to expand. If the water bath keeps its temperature (about 45?C) the room temperature is irrelevant. > Ren? J. > > --- On Tue, 2/14/12, wassan alkadhumi wrote: > > > From: wassan alkadhumi > Subject: [Histonet] wrinkles > To: "h n" > Date: Tuesday, February 14, 2012, 1:44 PM > > > Dear histonet members > First?I wish every one?a happy valentine day!!! > At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When?I trim the paraffin embedded tissue my sections?are faultless?.?Now because it is winter I have a problem having wrinkles in the sectionsafter collecting them from the water bath. Does anyone recommend me?to buy?slide drying hotplate for the slides?especially for the IHC slides, if you recommend me to buy it at what temperature should I use it and how it would effect the?baking time afterward? > > Thanks > Wassan/Histotechnican > Shorsh General?Hospital > North of Iraq > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 8 > Date: Tue, 14 Feb 2012 16:33:36 -0500 > From: Bob Richmond > Subject: [Histonet] QA by pathologists > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Akemi Allison asks: > >>>What % QA do most pathologists do internally? I'm trying to update my AP Manual. My last CLIA inspection, they told me to put in my AP Manual that it is done and get a binder labeled "discrepancies". ?If there are any...<< > > As far as I know, there is no percentage requirement like the > burdensome and useless 10% rescreen on Pap smears. In my travels I've > seen: > > One three-pathologist group did 100% review before sign-out. > > At least two services I've seen did a 10% review, cases chosen at > random. This seemed really pointless. > > One service did a 10% review, with the originating pathologist > choosing the cases to be reviewed. This fairly productive. > > One five-pathologist service meticulously documented informal > consultation, both in the report and on a separate piece of paper. > They aimed at, and got, a 2.8% review. In a multiple pathologist > practice, I think this was the most productive method I ever saw. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 9 > Date: Tue, 14 Feb 2012 16:31:29 -0600 > From: "Vickroy, Jim" > Subject: [Histonet] New DNA Probes for Kappa and Lambda > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<24A4826E8EF0964D86BC5317306F58A55FDC5DE697@mmc-mail.ad.mhsil.com> > Content-Type: text/plain; charset="us-ascii" > > In the past we have used a cocktail DNA probe (ISH) for Kappa and Lambda. ?Has anybody had any experience validating and setting up the new probes from Ventana which we have to mix the four probes together and form our own cocktail? ?Please share with me your thoughts. ? I am seriously considering sending this test out because of the infrequency, time, ASR hassle, costs of separate detection kits, etc. > > Jim > > James Vickroy BS, HT(ASCP) > > Surgical ?and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 10 > Date: Tue, 14 Feb 2012 23:04:54 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] (no subject) > To: "'Ingles Claire '" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <6D6BD1DE8A5571489398B392A38A715760A1AAFF@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > The reason for using diastase is to remove the glycogen from the tissues. AND if you do not heat the methenamine silver solution for the GMS it will take forever to stain, in fact all you will get is background spots. This I can verify, have you ever forgot to turn on the water bath before staining? - several hours later - nuthin!! > > Who ever is giving you the advice I would wonder about their histotechnology education and training (in fact have they had any training at all?). > > Do not listen to them. Extrapolate from cytology. GMS and DiPAS have been done on cytology smears fixed in ethanol as well as air-dried for years see: > > Loughman NT. "Pneumocystis carinii: rapid diagnosis with the microwave oven" Acta Cytologica. 33(3):416-7, 1989 May-Jun. > > PINTOZZI, R. L. "Modified Grocott's methenamine silver nitrate method for quick staining of Pneumocystis carinii" ?J Clin Pathol 1978 31: 803-805 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire > Sent: Wednesday, 15 February 2012 6:22 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hey histo gurus: > Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active "enzymes" in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. > Claire > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 11 > Date: Wed, 15 Feb 2012 08:14:10 +0200 > From: "Marilyn Tyler" > Subject: [Histonet] endogeneous peroxidase blocking > To: "histonet" > Message-ID: <4F3B6952020000900012F35B@gwiasmtp.uct.ac.za> > Content-Type: text/plain; charset="windows-1252" > > Morning All > > Posing this question for a colleague. Suggestions for > immunohistochemistry blocking for endogeneous peroxidase probably caused > by red blood cells in mouse lung tissue. Using 1% hydrogen > peroxide/dist.water for 15mins and still getting non specific staining. > > > Thank you > > Marilyn > > > Medical School UCT > Dept of Surgery J50/26 or 30 > Surgical research. OMB > Groote Schuur Hospital > Observatory > > 021-4066227 > > > > > > ### > > UNIVERSITY OF CAPE TOWN > > This e-mail is subject to the UCT ICT policies and e-mail disclaimer > published on our website at > http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from > +27 21 650 9111. This e-mail is intended only for the person(s) to whom > it is addressed. If the e-mail has reached you in error, please notify > the author. If you are not the intended recipient of the e-mail you may > not use, disclose, copy, redirect or print the content. If this e-mail > is not related to the business of UCT it is sent by the sender in the > sender's individual capacity. > > ### > > > ------------------------------ > > Message: 12 > Date: Wed, 15 Feb 2012 01:27:14 -0800 (PST) > From: Sheree H > Subject: [Histonet] (no subject) > To: dennis.barry@thrivent.com, lmphillips@charter.net, > ? ? ? ?melissa.zak-walser@jacobs.com, shiloy@charter.net, > ? ? ? ?histonet@lists.utsouthwestern.edu, skiptokatblack@bellsouth.net, > ? ? ? ?agianakas@bellsouth.net > Message-ID: > ? ? ? ?<1329298034.80174.YahooMailMobile@web125806.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > http://www.kingtigertkdwilson.com/modules/mod_wdbanners/myfriends.php?central164.gif > > ------------------------------ > > Message: 13 > Date: Wed, 15 Feb 2012 05:52:19 -0500 > From: Sheila Adey > Subject: [Histonet] Meditech users > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello:Are any of the Meditech users having the pathologists order their levels in the computer?We would like to implement this procedure. Any suggestions. :)ThanksSheila > > ------------------------------ > > Message: 14 > Date: Wed, 15 Feb 2012 09:43:50 -0600 > From: Stella Mireles > Subject: [Histonet] Independent Service Technician > To: Histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Is there anyone out there that does repairs on Sakura equipment and works > for themselves > in the Texas area. > > > ------------------------------ > > Message: 15 > Date: Wed, 15 Feb 2012 10:49:27 -0600 > From: "Reynolds,Donna M" > Subject: [Histonet] Re: bone implants > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<785BBF0C5F49CE41BA74460A43A08F023055EE4325@DCPWVMBXC0VS3.mdanderson.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > I don't know about implants but we were having a terrible time with bone marrows coming off. Usually they came off during the HEIR using the steamer. > At that point we were placing our slides lying flat in a 55-60? oven overnight which usually helps tremendously with difficult tissue. This was followed with routine deparaffinization and hydration. It was suggested to us to try a 70? water bath for 2-4 hours for HEIR. I was doubtful that my antibodies would work but they did. In fact the staining was better with 2 hr HEIR than with 4. > > ?Donna Reynolds Chief Histology laboratory, Research > Department Cancer Biology > U.T. M.D. Anderson Cancer Center, Houston, TX. > 713-792-8106 > > Message: 4 > Date: Mon, 13 Feb 2012 09:53:15 -0600 > From: "del phillips" > Subject: [Histonet] IHC and bone implants > To: > Message-ID: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Does anyone have any suggestions for staining bone implants? I am having > trouble with wash offs. I use charged slides and they are still washing off. > > > > Thanks > > Del > > > > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 19 > **************************************** From Marilyn.Tyler <@t> uct.ac.za Thu Feb 16 02:39:55 2012 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Thu Feb 16 02:40:41 2012 Subject: [Histonet] Thanks Message-ID: <4F3CDCFB020000900012FCCB@gwiasmtp.uct.ac.za> Hi To All A big thank you to everyone for their input regarding endogeous peroxidase blocking. Much appreciated Marilyn Medical School UCT Dept of Surgery J50/26 or 30 Surgical research. OMB Groote Schuur Hospital Observatory 021-4066227 ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From TMcNemar <@t> lmhealth.org Thu Feb 16 05:16:19 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Feb 16 05:16:11 2012 Subject: [Histonet] Ribbon won't stay on knife In-Reply-To: References: Message-ID: I use my forceps to round over the top edge of the block when this happens. If you ever have trouble getting a ribbon to form because the sections do not stick together, you can also use the forceps to straighten the bottom edge of the block. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Wednesday, February 15, 2012 6:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ribbon won't stay on knife Sometimes while cutting 5 micron paraffin sections... I turn the wheel once and a nice section gets cut and lays down on my blade. When I turn the wheel again, as the knife holder is traveling Upwards, the top of my paraffin block will grab the ribbon and lift it off of the microtome blade, ruining my ribbon. Any ideas what is going on, how to fix it. Today I'm using my old trust Spencer 820 microtome, but it also sometimes happens on my Leica RM2125. Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sprice2003 <@t> gmail.com Thu Feb 16 07:16:27 2012 From: sprice2003 <@t> gmail.com (Sally Price) Date: Thu Feb 16 07:16:35 2012 Subject: [Histonet] New KOH-type stain Message-ID: Histonetters: The other day I recieved by mail a brochure for a stain in a one-gallon bottle that claimed it stained fungal elements bluish-purple and helped reading KOH's. I gave the brochure to someone and they seem to have misplaced it. It wasn't the Chicago Sky Blue stain that's in those kits (and is very expensive, 1 oz. bottles for $70). Has anyone else received this info and if so could they share the vendor/product info? Cheers, Sally From trathborne <@t> somerset-healthcare.com Thu Feb 16 08:21:24 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Feb 16 08:21:14 2012 Subject: [Histonet] Ribbon won't stay on knife In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F8337B@SMCMAIL01.somerset-healthcare.com> I also find it helpful to blunt the bottom edge of the block by scraping to create two corners/angles on it instead of one 90 degree angle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 16, 2012 6:16 AM To: 'Jerry Ricks'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ribbon won't stay on knife I use my forceps to round over the top edge of the block when this happens. If you ever have trouble getting a ribbon to form because the sections do not stick together, you can also use the forceps to straighten the bottom edge of the block. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Wednesday, February 15, 2012 6:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ribbon won't stay on knife Sometimes while cutting 5 micron paraffin sections... I turn the wheel once and a nice section gets cut and lays down on my blade. When I turn the wheel again, as the knife holder is traveling Upwards, the top of my paraffin block will grab the ribbon and lift it off of the microtome blade, ruining my ribbon. Any ideas what is going on, how to fix it. Today I'm using my old trust Spencer 820 microtome, but it also sometimes happens on my Leica RM2125. Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From moteuncle <@t> gmail.com Thu Feb 16 08:27:54 2012 From: moteuncle <@t> gmail.com (mote uncle) Date: Thu Feb 16 08:28:02 2012 Subject: [Histonet] Mice heads in Paraffin Message-ID: Hello This is my first post to this site. I have encountered a problem with embedding of mice heads in Paraffin (Gurr). Initially with Embryonic day 13-14, the protocol was working properly and the sections were also nice but with Embryonic day 17-19 I could see even after several hours of ethanol wash and xylene treatment the mice heads were soft but still I embedded the samples in paraffin. When I removed the paraffin blocks from the mold I could see the mice portion to be white, some flecks of wax coming out of surface and the surface of wax had some cracks. I could not rule out what was causing the problem. Protocol I used for E 17-19 After Fixing in EDTA (2 weeks) and putting the mice heads in running water for 48 hours, 50% Ethanol 3 hours 70% Ethanol 3 hours 95% Ethanol 3 hours 100% Ethanol 3 hours 100% Ethanol 3 hours Xylene 2-4 hrs (till clearing of samples was observed) Paraffin 30 mins Paraffin 30 mins Paraffin 30 mins Paraffin 30 mins Could anybody share me your protocol for Embryonic day 17-19 and Post natal days. Regards Mote From Carol.Freeman <@t> utoledo.edu Thu Feb 16 08:56:49 2012 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Thu Feb 16 08:57:56 2012 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Good Morning Histonet, Ok, We are just starting up with MSI testing in our facility and a question has popped up about diagnosis of these slides... Are you looking for a COMPLETE LOSS of this protein in the tumor, or just a loss of some expression.? So if you have a normal piece of tissue staining uniform and then a tumor of that same tissue showing vague patchy staining in some cells and a loss in other cells of interest are you considering that a loss or not. Does the tumor have to show a COMPLETE loss of this protein... Any answer is appreciated. From Ronald.Houston <@t> nationwidechildrens.org Thu Feb 16 09:13:10 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Feb 16 09:13:24 2012 Subject: [Histonet] Interfacing CoPath with clients Message-ID: Does anyone have experience interfacing Sunquest CoPath with external clients so that they are able to access reports at will? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Rcartun <@t> harthosp.org Thu Feb 16 09:15:18 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 16 09:15:33 2012 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <4F3CD736.7400.0077.1@harthosp.org> Complete loss. You can see patchy immunoreactivity in tumors with these markers. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Freeman, Carol" 2/16/2012 9:56 AM >>> Good Morning Histonet, Ok, We are just starting up with MSI testing in our facility and a question has popped up about diagnosis of these slides... Are you looking for a COMPLETE LOSS of this protein in the tumor, or just a loss of some expression.? So if you have a normal piece of tissue staining uniform and then a tumor of that same tissue showing vague patchy staining in some cells and a loss in other cells of interest are you considering that a loss or not. Does the tumor have to show a COMPLETE loss of this protein... Any answer is appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Feb 16 09:27:05 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Feb 16 09:26:52 2012 Subject: [Histonet] IHC and negative controls? Message-ID: Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From nto <@t> stowers.org Thu Feb 16 09:29:08 2012 From: nto <@t> stowers.org (Thomas, Nancy) Date: Thu Feb 16 09:29:21 2012 Subject: [Histonet] Mice heads in Paraffin In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF996D1B891@EXCHMB-02.stowers-institute.org> Mote, For the E17-19, we found that they need more time in paraffin. We have 2 hours in each of the dehydration and clearing steps and two hours in each of the 4 paraffin baths. This is 8 hours in paraffin, but for this larger size, that is what we found to work. Nancy Thomas Stowers Institute for Medical Research Kansas City, MO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mote uncle Sent: Thursday, February 16, 2012 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mice heads in Paraffin Hello This is my first post to this site. I have encountered a problem with embedding of mice heads in Paraffin (Gurr). Initially with Embryonic day 13-14, the protocol was working properly and the sections were also nice but with Embryonic day 17-19 I could see even after several hours of ethanol wash and xylene treatment the mice heads were soft but still I embedded the samples in paraffin. When I removed the paraffin blocks from the mold I could see the mice portion to be white, some flecks of wax coming out of surface and the surface of wax had some cracks. I could not rule out what was causing the problem. Protocol I used for E 17-19 After Fixing in EDTA (2 weeks) and putting the mice heads in running water for 48 hours, 50% Ethanol 3 hours 70% Ethanol 3 hours 95% Ethanol 3 hours 100% Ethanol 3 hours 100% Ethanol 3 hours Xylene 2-4 hrs (till clearing of samples was observed) Paraffin 30 mins Paraffin 30 mins Paraffin 30 mins Paraffin 30 mins Could anybody share me your protocol for Embryonic day 17-19 and Post natal days. Regards Mote _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Candy.A.Bales <@t> uth.tmc.edu Thu Feb 16 09:31:21 2012 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Thu Feb 16 09:31:25 2012 Subject: [Histonet] tissue processors Message-ID: <62C915811DD5A142851D95CA6BC5D1E423E46FCC28@UTHCMS1.uthouston.edu> Good morning. These questions are on behalf of a friend who is in the market for a new tissue processor. She wants to know the pros and cons of the various processors in use. She mentioned the Excelsior, VIP & Leica but is open to other suggestions. The workload is approximately 200 blocks or less per night. She is looking for something the doctors can easily stop and add cassettes to and restart. She is also looking to replace her old linear stainer, no longer being produced by Thermo-Shandon. Does anyone know of a company producing a linear stainer? Or for those who have auto stainers, if you could give her the pros and cons of the models you use. Thanking you in advance Candy Bales, HT Chief Histologist The University of Texas Health Science Center Houston- School of Dentistry Diagnostic & Biomedical Sciences 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From tajibade <@t> echd.org Thu Feb 16 09:39:42 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Thu Feb 16 09:40:00 2012 Subject: [Histonet] RE: IHC and negative controls? In-Reply-To: References: Message-ID: I am not sure if they can do that because CAP ANP.22570, explain this. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 16, 2012 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From settembr <@t> umdnj.edu Thu Feb 16 09:51:22 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Thu Feb 16 09:51:34 2012 Subject: [Histonet] RE: IHC and negative controls? In-Reply-To: References: Message-ID: Tom, As Tunde says, the Checklist 7/11/2011 question ANP.22570 does explain that you can do as your pathologists suggests, BUT there are rules to be followed if that is what you will do. "A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks ... 2. The positive control slide or the patient test slides, if these slides contain tissue elements that should not react with the antibody. [this is the one you want to follow] 3. A separate negative tissue control slide. The type of negative tissue control used ... SHOULD BE SPECIFIED in the laboratory manual. ... Evidence of Compliance: Written procedure of the selection and use of ... tissue controls for IHC..." Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade Sent: Thursday, February 16, 2012 10:40 AM To: 'histonet@lists.utsouthwestern.edu' Cc: 'histonet-bounces@lists.utsouthwestern.edu' Subject: [Histonet] RE: IHC and negative controls? I am not sure if they can do that because CAP ANP.22570, explain this. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 16, 2012 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Thu Feb 16 10:01:53 2012 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Feb 16 10:02:04 2012 Subject: [Histonet] AUTO: Laura Miller is Out of the Office. (returning 04/16/2012) Message-ID: I am out of the office until 04/16/2012. I will be on medical leave until April 16, 2012. Please contact David Archambault at 847-405-5017 if you need assistance. Thank you. Note: This is an automated response to your message "Histonet Digest, Vol 99, Issue 20" sent on 2/16/2012 8:55:31 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From TMcNemar <@t> lmhealth.org Thu Feb 16 10:29:56 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Feb 16 10:29:43 2012 Subject: [Histonet] RE: IHC and negative controls? In-Reply-To: References: Message-ID: Thanks to everyone for the quick responses. I am looking at ANP.22580 rev 12/12/2006 and it clearly talks about "a separate section of patient tissue..." but just a little further it talks about using internal negative controls ("The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250).") I did not see anything useful in ANP.22250. We are JCAHO accredited not CAP but I cannot find any JCAHO discussion on the subject. Personally, I believe that we should run separate negative controls for each case. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 16, 2012 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From faith14913 <@t> aol.com Thu Feb 16 10:16:40 2012 From: faith14913 <@t> aol.com (faith14913@aol.com) Date: Thu Feb 16 10:35:12 2012 Subject: [Histonet] (no subject) Message-ID: <8CEBADD7F6F9950-AB4-329E@webmail-m148.sysops.aol.com> http://phamsp.org/wp-content/plugins/extended-comment-options/test.php?flag128.mp3 From srhthacker <@t> hotmail.com Thu Feb 16 11:31:27 2012 From: srhthacker <@t> hotmail.com (Stephanie Hoyle-Thacker) Date: Thu Feb 16 11:31:35 2012 Subject: [Histonet] Re:1 Message-ID: ..Make alot of money using your PC http://misosofos.com/day.link.php?qositeid=66vy1 From darlene.giracello <@t> okstate.edu Thu Feb 16 11:46:45 2012 From: darlene.giracello <@t> okstate.edu (Giracello, Darlene) Date: Thu Feb 16 11:46:57 2012 Subject: [Histonet] Opinions on Leica stainer Message-ID: <0C2BDF59DC80E64AB2A59939D703B2E50180B3B7FE2F@STWEXE3.ad.okstate.edu> We would appreciate any opinions on the Leica Autostainer XL ST5010 and the ST5020. Any likes, dislikes, has it been reliable? Consumables? Service? We are a veterinary diagnostic lab, so flexibility is very important for us. I bow to the wisdom of Histonet!!! Thanks Darlene From Ronald.Houston <@t> nationwidechildrens.org Thu Feb 16 11:57:08 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Feb 16 11:57:19 2012 Subject: [Histonet] charging for muscle biopsy Message-ID: What CPT codes would you use for a muscle biopsy sample that was dissected for frozen enzyme histochemistry, paraffin and EM? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From JWeems <@t> sjha.org Thu Feb 16 12:04:06 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Feb 16 12:04:18 2012 Subject: [Histonet] RE: charging for muscle biopsy In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640846ED9F27@CHEXCMS10.one.ads.che.org> This is what our reference lab uses... MUSCLE BX SDH -PATH REF 88319 MUSCLE BX COX -PATH REF 88319 MUSCLE BX ATP 4.3 -PATH REF 88319 MUSCLE BX ATP 9.4 -PATH REF 88319 MUSCLE BX CD8+T LYMPH -PATH REF 88342 MUSCLE BX STAIN MAJOR HISTO -PATH REF 88342 MUSCLE BX IP VIMENTIN -PATH REF 88342 MUSCLE BX NEURAL CELL ADHESION-PATH REF 88342 MUSCLE BX IP C5b 9 -PATH REF 88342 MUSCLE BX CD79+B CELLS -PATH REF 88342 MUSCLE BX CD68+MACROPHA-PATH REF 88342 MUSCLE BX LEVEL IV - PATH 88305 MUSCLE BX CONGO RED - PATH 88313 MUSCLE BX TRICHROME - PATH 88314 MUSCLE BX OIL RED O - PATH 88314 MUSCLE BX PAS - PATH 88314 MUSCLE BX ATP - 4.2 - PATH 88319 MUSCLE BX ATP - 10.4 - PATH 88319 MUSCLE BX NADH - PATH 88319 MUSCLE BX SDH - PATH 88319 MUSCLE BX CYTOCHROME C - PATH 88319 MUSCLE BX COX/SDH - PATH 88319 MUSCLE BX ACID PHOS - PATH 88319 ELECTRON MICROSCOPY -PATH REF 88348 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, February 16, 2012 12:57 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] charging for muscle biopsy Importance: High What CPT codes would you use for a muscle biopsy sample that was dissected for frozen enzyme histochemistry, paraffin and EM? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From dphillips <@t> vetmed.lsu.edu Thu Feb 16 12:29:37 2012 From: dphillips <@t> vetmed.lsu.edu (del phillips) Date: Thu Feb 16 12:31:40 2012 Subject: [Histonet] Ventana xt Message-ID: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu> Looking for any pros and cons on the Ventana XT. Thanks Del From Rcartun <@t> harthosp.org Thu Feb 16 12:37:36 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 16 12:37:51 2012 Subject: [Histonet] Brucellosis Message-ID: <4F3D06A0.7400.0077.1@harthosp.org> I have a consult (transplant kidney biopsy) that I believe is infected with Brucella. My IHC test is positive, but I have never seen a human case before. Can someone familiar with this disease forward me a photo or a good reference that shows the typical morphology of this organism in human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From trathborne <@t> somerset-healthcare.com Thu Feb 16 13:03:57 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Feb 16 13:03:44 2012 Subject: [Histonet] RE: Opinions on Leica stainer In-Reply-To: <0C2BDF59DC80E64AB2A59939D703B2E50180B3B7FE2F@STWEXE3.ad.okstate.edu> References: <0C2BDF59DC80E64AB2A59939D703B2E50180B3B7FE2F@STWEXE3.ad.okstate.edu> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F834D9@SMCMAIL01.somerset-healthcare.com> We love our ST5020. The ovens are a great feature. Very flexible. Leica service has been very good, especially this past year - I think they made some changes to their dispatch. Consumables will depend on who you get your reagents from. We have the CV5030 coverslipper attached, so the "load it and walk away" feature is nice to have. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giracello, Darlene Sent: Thursday, February 16, 2012 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on Leica stainer We would appreciate any opinions on the Leica Autostainer XL ST5010 and the ST5020. Any likes, dislikes, has it been reliable? Consumables? Service? We are a veterinary diagnostic lab, so flexibility is very important for us. I bow to the wisdom of Histonet!!! Thanks Darlene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From hsmith <@t> wakehealth.edu Thu Feb 16 13:14:32 2012 From: hsmith <@t> wakehealth.edu (Hilary Smith) Date: Thu Feb 16 13:14:52 2012 Subject: [Histonet] Myelin staining of fresh-frozen tissue Message-ID: Hi Histonetters Has anyone had success with Weil's myelin stain in fresh-frozen tissue? And what is my best bet for post-fixation prior to staining? Will 4% paraformaldehyde do the trick? Presumably for a myelin stain I shouldn't defat? Thanks, Hilary Hilary Smith Research Assistant Porrino Neuroimaging Lab Department of Physiology and Pharmacology Wake Forest School of Medicine Medical Center Boulevard Winston-Salem, NC 27157 Phone 336.716.8649 Fax 336.716.8689 From Elizabeth.Cameron <@t> jax.org Thu Feb 16 13:17:13 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Thu Feb 16 13:17:22 2012 Subject: [Histonet] Jones/PAMS Message-ID: Hi, I was wondering if anyone has any suggestions for a Jones/PAMS stain that is not working properly. This is something we don't do often. The last time we did it was a year and a half ago, and it seemed fine at the time. We have tried 3 or 4 protocols, including an ammoniacal silver, and it is still not working properly. In some protocols, our red cells are staining but the capillaries in the glomeruli do not seem to be picking up the silver. In other protocols, there are nuclei of some cells that should not be staining that are, but again, the capillaries are not. We are working on mouse tissue that is fixed in NBF. The strange thing is the stain seems to be working well on Bouins and Telly's fixed tissue. I even tried mordanting in Bouins! We have tried multiple kidneys with the same results. We are on new bottles of silver and periodic acid, although our methenamine has been around a while. Any suggestions would be greatly appreciated. Thanks! Liz From HornHV <@t> archildrens.org Thu Feb 16 13:18:36 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Feb 16 13:18:47 2012 Subject: [Histonet] Meditech users - pathologists ordering levels In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719B2692A15@EVS1.archildrens.org> I have never seen any pathologist I have worked for look at a block before asking for recuts. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 15, 2012 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech users - pathologists ordering levels Sheila asks: >>Hello: Are any of the Meditech users having the pathologists order >>their levels in the computer? We would like to implement this >>procedure. Any suggestions?<< Never seen this - but it sounds like a nifty little time-waster for all concerned. I usually examine the paraffin block before ordering levels or other recuts - to make sure the block isn't exhausted or horrible. Am I the only pathologist who does this? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From trathborne <@t> somerset-healthcare.com Thu Feb 16 13:31:37 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Feb 16 13:31:24 2012 Subject: [Histonet] Meditech users - pathologists ordering levels In-Reply-To: <25A4DE08332B19499904459F00AAACB719B2692A15@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719B2692A15@EVS1.archildrens.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F8357A@SMCMAIL01.somerset-healthcare.com> Only occasionally. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, February 16, 2012 2:19 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Meditech users - pathologists ordering levels I have never seen any pathologist I have worked for look at a block before asking for recuts. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 15, 2012 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech users - pathologists ordering levels Sheila asks: >>Hello: Are any of the Meditech users having the pathologists order >>their levels in the computer? We would like to implement this >>procedure. Any suggestions?<< Never seen this - but it sounds like a nifty little time-waster for all concerned. I usually examine the paraffin block before ordering levels or other recuts - to make sure the block isn't exhausted or horrible. Am I the only pathologist who does this? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. 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From christiegowan <@t> msn.com Thu Feb 16 13:46:35 2012 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Feb 16 13:46:44 2012 Subject: [Histonet] Ventana xt In-Reply-To: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu> References: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu> Message-ID: Pros: Lends itself well to labs that do basic IHC staining Antibodies are pre-diluted so no guess work on dilutions Good for batching runs Consistent quality of slides 24 hour technical support Can run molecular probes protocols are easy to adjust Cons: Closed system Must use Ventana antibodies or purchase special dispensers if using non Ventana products Pre-dilute antibodies are pricey Stand alone instrument so must have space for it I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. Christie Gowan UAB Hospital > From: dphillips@vetmed.lsu.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 16 Feb 2012 12:29:37 -0600 > Subject: [Histonet] Ventana xt > > Looking for any pros and cons on the Ventana XT. > > > > Thanks > > Del > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Thu Feb 16 14:41:35 2012 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Feb 16 14:41:44 2012 Subject: [Histonet] Ventana XT Closed System misnomer Message-ID: Responding to Del who asked about pros/cons of Ventana BenchMark XT: Ventana is often equated with 'closed system'. This is a misnomer. The only thing I regard as closed (besides some colleagues' minds on this issue) is the detection system kit and bulk reagents that are used on the instrument. While these are proprietary, they are also of extremely high quality, resiliency, consistency, and in my opinion, dependability. Preparing one's own reagents can lead to a lack of consistency of staining, and we have confidence in Ventana reagents. As others have correctly noted...other non-Ventana antibodies can be used in Ventana dispensers. We use an equal mix of ready-to-use antibodies with our own diluted antibodies from other suppliers. How is this a closed system? Pre-treatments can also be used in Ventana dispensers. There is a range of non-ab reagents such as hematoxylins and proteases. There are a range of detection system kits as well - at least four that I can think of. Perhaps this sounds like my personal crusade, as a Ventana user; to try to demolish the 'closed system' myth. In my professional interactions with non-Ventana users, this is often heard as a derogatory, negative reaction to Ventana, which I certainly speak truth to at every opportunity. Del, we have found the BenchMark XT's very reliable, with good support and service, plus excellent quality reagents including antibodies. Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingson, Ontario, Canada From Lynn.Burton <@t> Illinois.gov Thu Feb 16 14:42:29 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Feb 16 14:44:31 2012 Subject: [Histonet] Ventana xt In-Reply-To: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu> References: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00649C40AC1@IL084EXMBX214.illinois.gov> One great thing is that it does everything on the machine. It deparrifinizes as well as stains. The ultra view platform has even simplified the steps from machine to coverslipping. It can be set to run overnight. It has delayed timers for starting or ending. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of del phillips [dphillips@vetmed.lsu.edu] Sent: Thursday, February 16, 2012 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana xt Looking for any pros and cons on the Ventana XT. Thanks Del _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mfisher <@t> ecrmc.org Thu Feb 16 17:23:03 2012 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu Feb 16 17:23:17 2012 Subject: [Histonet] CPT Code In-Reply-To: <20120212180400.8F4681BC04D7_F37FF10F@sophos.ecrmc.ci.el-centro.ca.us> References: <20120212180400.8F4681BC04D7_F37FF10F@sophos.ecrmc.ci.el-centro.ca.us> Message-ID: 88365 x 2: Kappa/Lambda by ISH Does this code qualify for charges for bone marrows, fresh tissue and/or paraffin embedded? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ? Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, February 12, 2012 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 99, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Formalin training (Ann Angelo) 2. Anti Static Guns (Marcia Fisher) 3. Invitation from HistoTALK (David Kemler) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Feb 2012 13:20:04 -0500 (EST) From: Ann Angelo Subject: [Histonet] Formalin training To: histonet@lists.utsouthwestern.edu Message-ID: <8CEB700E9485795-1A90-4C7D@webmail-m165.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a training program/presentation that can be used to train employees about 10% NBF in the Histology lab? Ann ------------------------------ Message: 2 Date: Sat, 11 Feb 2012 18:29:06 +0000 From: Marcia Fisher Subject: [Histonet] Anti Static Guns To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use ZeroStat Antistatic Guns in our lab. Originally designed to remove fine dust particles from vinyl records (remember those?), I first began using them back in the 80's in our EM lab. When I arrived here 3 years ago and living in the desert where we have virtually no humidity, especially right now, I brought in my own personal gun (yes, I still have vinyls!) and introduced it to the lab. This gun is way over 20 years old and still works. If you google these, you will find them out there. But search around for the best price. You used to be able to walk in to a record store and buy them for about $10 but those stores are now obsolete too. I found some good prices on eBay. No chemicals, no harm to the environment, no smell, no mess! Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ? Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, February 11, 2012 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 99, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: anti static spray (Rick Tiefenauer) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Feb 2012 08:53:35 -0800 From: Rick Tiefenauer Subject: Re: [Histonet] anti static spray To: liz@premierlab.com Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Liz During my Navy days to help prevent static problems off X-ray films, we used a 95(ish)% isopropyl and water mix in a squeeze spray bottle. A very light mist before opening the paper film holders, and allowing it to completely evaporate, normally did the trick. I know of one histo lab here in Cali. that does a light spray of ethyl mix periodically on their blocks while chilling before microtomy, don't think they have much static issues. Watch out for over the-counter sprays from a local store, many have Butane and/or Propane (refrigerant types), and some have 1,1-Difluoroethane (normally found in computer canned air). Many have some type of fluorine-byproducts because of the high electronegativity. Cotton lab coats, and the humidity trick are also helpful. Good luck. Rick T. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 99, Issue 15 **************************************** ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ------------------------------ Message: 3 Date: Sun, 12 Feb 2012 06:29:48 -0800 (PST) From: David Kemler Subject: [Histonet] Invitation from HistoTALK To: Fellow HistoNetters Message-ID: <1329056988.63186.YahooMailNeo@web120606.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Everyone - You are all invited to join me tonight, February 12th?at 10 pm Eastern for HistoTALK www.HistoTALK.com. Tonight's guest is Lamar Jones, Technical Coordinator Pathology?from Emory University Hospital in Atlanta, GA.?HistoTALK is?a bi-monthly?"Podcast" which?is available 24/7/365. ? Yours, Dave ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 99, Issue 16 **************************************** ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From Susan.Walzer <@t> HCAHealthcare.com Fri Feb 17 02:12:05 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Feb 17 02:12:15 2012 Subject: [Histonet] RE: Opinions on Leica stainer In-Reply-To: <0C2BDF59DC80E64AB2A59939D703B2E50180B3B7FE2F@STWEXE3.ad.okstate.edu> References: <0C2BDF59DC80E64AB2A59939D703B2E50180B3B7FE2F@STWEXE3.ad.okstate.edu> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DE2541744@FWDCWPMSGCMS09.hca.corpad.net> Workhorse...has all the requirements we need. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giracello, Darlene Sent: Thursday, February 16, 2012 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on Leica stainer We would appreciate any opinions on the Leica Autostainer XL ST5010 and the ST5020. Any likes, dislikes, has it been reliable? Consumables? Service? We are a veterinary diagnostic lab, so flexibility is very important for us. I bow to the wisdom of Histonet!!! Thanks Darlene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Fri Feb 17 04:47:25 2012 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Feb 17 04:47:35 2012 Subject: [Histonet] RE: Opinions on Leica stainer In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DE2541744@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40DB7@isexstore03> We love ours!! Works great!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Friday, February 17, 2012 3:12 AM To: darlene.giracello@okstate.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Opinions on Leica stainer Workhorse...has all the requirements we need. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Giracello, Darlene Sent: Thursday, February 16, 2012 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on Leica stainer We would appreciate any opinions on the Leica Autostainer XL ST5010 and the ST5020. Any likes, dislikes, has it been reliable? Consumables? Service? We are a veterinary diagnostic lab, so flexibility is very important for us. I bow to the wisdom of Histonet!!! Thanks Darlene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From pablo.sanchez <@t> usc.es Fri Feb 17 05:13:32 2012 From: pablo.sanchez <@t> usc.es (Pablo Sanchez-Quinteiro) Date: Fri Feb 17 05:13:40 2012 Subject: [Histonet] Cutting Tendon Message-ID: Dear listers, I am trying to get transversal sections of cow flexor digital tendons. I need sections of the whole piece, about 1cm diameter. Before paraffin embedding I have treated the samples with: - KOH 4% - 4% phenol in 70% ethanol In both cases the results are very poor; the tissue is torn to pieces. I would very grateful for any input regarding this issue. Best Regards Pablo From mamawooo <@t> hotmail.com Fri Feb 17 07:52:09 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Feb 17 07:52:16 2012 Subject: [Histonet] Ventana xt In-Reply-To: References: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu>, Message-ID: I think the Ventana XT is the best instrument for adding slides as you go which makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is so easy to use it is virtually impossible to mess up with it's bar coding and visual control. Very user friendly. Jan Mahoney,Omaha, NE > From: christiegowan@msn.com > To: dphillips@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu > Date: Thu, 16 Feb 2012 19:46:35 +0000 > Subject: RE: [Histonet] Ventana xt > CC: > > > Pros: > Lends itself well to labs that do basic IHC staining > Antibodies are pre-diluted so no guess work on dilutions > Good for batching runs > Consistent quality of slides > 24 hour technical support > Can run molecular probes > protocols are easy to adjust > > Cons: > Closed system > Must use Ventana antibodies or purchase special dispensers if using non Ventana products > Pre-dilute antibodies are pricey > Stand alone instrument so must have space for it > > I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. > Christie Gowan > UAB Hospital > > > > > From: dphillips@vetmed.lsu.edu > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 16 Feb 2012 12:29:37 -0600 > > Subject: [Histonet] Ventana xt > > > > Looking for any pros and cons on the Ventana XT. > > > > > > > > Thanks > > > > Del > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Feb 17 08:23:33 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Feb 17 08:23:40 2012 Subject: [Histonet] Ventana xt In-Reply-To: References: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu>, Message-ID: Side note: only the Ventana Ultra allows adding slides as you go, not the XT. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, February 17, 2012 7:52 AM To: Christie Gowan; dphillips@vetmed.lsu.edu; histonet Subject: RE: [Histonet] Ventana xt I think the Ventana XT is the best instrument for adding slides as you go which makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is so easy to use it is virtually impossible to mess up with it's bar coding and visual control. Very user friendly. Jan Mahoney,Omaha, NE > From: christiegowan@msn.com > To: dphillips@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu > Date: Thu, 16 Feb 2012 19:46:35 +0000 > Subject: RE: [Histonet] Ventana xt > CC: > > > Pros: > Lends itself well to labs that do basic IHC staining Antibodies are > pre-diluted so no guess work on dilutions Good for batching runs > Consistent quality of slides > 24 hour technical support > Can run molecular probes > protocols are easy to adjust > > Cons: > Closed system > Must use Ventana antibodies or purchase special dispensers if using > non Ventana products Pre-dilute antibodies are pricey Stand alone > instrument so must have space for it > > I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. > Christie Gowan > UAB Hospital > > > > > From: dphillips@vetmed.lsu.edu > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 16 Feb 2012 12:29:37 -0600 > > Subject: [Histonet] Ventana xt > > > > Looking for any pros and cons on the Ventana XT. > > > > > > > > Thanks > > > > Del > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 17 08:48:39 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 17 08:48:47 2012 Subject: [Histonet] tissue processors In-Reply-To: <62C915811DD5A142851D95CA6BC5D1E423E46FCC28@UTHCMS1.uthouston.edu> Message-ID: <1329490119.49774.YahooMailClassic@web162102.mail.bf1.yahoo.com> For your requirements, Sakura VIP is the bast, at least for me. Ren? J. --- On Thu, 2/16/12, Bales, Candy A wrote: From: Bales, Candy A Subject: [Histonet] tissue processors To: "histonet@lists.utsouthwestern.edu" Date: Thursday, February 16, 2012, 10:31 AM Good morning. These questions are on behalf of a friend who is in the market for a new tissue processor. She wants to know the pros and cons of the various processors in use. She mentioned the Excelsior, VIP & Leica but is open to other suggestions. The workload is approximately 200 blocks or less per night. She is looking for something the doctors can easily stop and add cassettes to and restart. She is also looking to replace her old linear stainer, no longer being produced by Thermo-Shandon.? Does anyone know of a company producing a linear stainer? Or for those who have auto stainers, if you could give her the pros and cons of the models you use. Thanking you in advance Candy Bales, HT Chief Histologist The University? of Texas Health Science Center Houston- School of Dentistry Diagnostic & Biomedical Sciences 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 17 08:53:18 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 17 09:00:04 2012 Subject: [Histonet] Cutting Tendon In-Reply-To: Message-ID: <1329490398.97847.YahooMailClassic@web162103.mail.bf1.yahoo.com> First of all fixation has to be perfect, if with NBF, at least 72 hours. Then increase twice?your standard processing times, specially infiltration. Substitute your normal paraffin was to one of 63-65?C and chill be blocks before sectioning. Ren? J. --- On Fri, 2/17/12, Pablo Sanchez-Quinteiro wrote: From: Pablo Sanchez-Quinteiro Subject: [Histonet] Cutting Tendon To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 6:13 AM ???Dear listers, ???I am trying to get transversal sections of cow flexor digital tendons. ???I? need? sections? of? the? whole? piece,? about? 1cm diameter. Before ???paraffin embedding I have treated the samples with: ???-? ? ? ? KOH 4% ???-? ? ? ? 4% phenol in 70% ethanol ???In both cases the results are very poor; the tissue is torn to pieces. ???I would very grateful for any input regarding this issue. ???Best Regards ???Pablo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Fri Feb 17 09:08:23 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Feb 17 09:08:44 2012 Subject: [Histonet] New Position Message-ID: <6F33D8418806044682A391273399860F0BE35E92@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From Pam.Bakken <@t> childrensmn.org Fri Feb 17 09:53:50 2012 From: Pam.Bakken <@t> childrensmn.org (Pam Bakken) Date: Fri Feb 17 09:54:02 2012 Subject: [Histonet] Union positions? Message-ID: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From W.E.J.Hoekert <@t> olvg.nl Fri Feb 17 09:54:16 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Feb 17 09:54:43 2012 Subject: [Histonet] IHC and negative controls? References: Message-ID: <1190CB05C44B13409483514729C2FC3601F84224@PAIT42.olvg.nl> We are not using negative controls. We only add positive controls at the bottom of our patient slides. Sometimes there are 3 different types of control tissue (a TMA), so that you can use the same control block for several antibodies. You could add a negative control in the same block as the positive control and add these at the bottom of your slides (or at the top, if you wish). Willem Hoekert OLVG The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Tom McNemar Verzonden: do 16-2-2012 16:27 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org > ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From eridana <@t> cox.net Fri Feb 17 10:30:48 2012 From: eridana <@t> cox.net (Eridana) Date: Fri Feb 17 10:30:52 2012 Subject: [Histonet] Anti static gun In-Reply-To: <20120217155634.NLWB22920.fed1rmfepi105.cox.net@fed1rmimpi01.cox.net> Message-ID: <20120217113048.4Y2M8.352933.imail@fed1rmwml304> Available through VWR, but may be cheaper elsewhere. They work much better than I expected. ZEROSTAT ANTISTATIC GUN VWR cat #100496-120 Vendor cat #60610 Each $122.64 list Donna Harclerode, HTL, QIHC, SLS (ASCP) Associate Scientist Celgene 9393 Towne Center Road San Diego, CA 92121 From we3smitty <@t> yahoo.com Fri Feb 17 10:46:06 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Fri Feb 17 10:46:10 2012 Subject: [Histonet] Union positions? In-Reply-To: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> Message-ID: <1329497166.77420.YahooMailClassic@web125406.mail.ne1.yahoo.com> I am a lab of 5 histotechs and no union. I would never accept a position that is union! --- On Fri, 2/17/12, Pam Bakken wrote: From: Pam Bakken Subject: [Histonet] Union positions? To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 10:53 AM Trying to put together some numbers, any and all responses to these questions would be greatly appreciated.? How many HT's are in union positions?? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Feb 17 11:13:02 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Feb 17 11:13:12 2012 Subject: [Histonet] Union positions? In-Reply-To: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> References: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB6FE860@LRGHEXVS1.practice.lrgh.org> Union position? Only if I was starving. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Bakken Sent: Friday, February 17, 2012 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Union positions? Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Feb 17 11:16:21 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Feb 17 11:17:31 2012 Subject: [Histonet] Union positions? In-Reply-To: <1329497166.77420.YahooMailClassic@web125406.mail.ne1.yahoo.com> References: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org>, <1329497166.77420.YahooMailClassic@web125406.mail.ne1.yahoo.com> Message-ID: We have 17 histotech over 1000 lab techs ( classified as Medical Technologists) and NO union Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith [we3smitty@yahoo.com] Sent: Friday, February 17, 2012 11:46 AM To: histonet@lists.utsouthwestern.edu; Pam Bakken Subject: Re: [Histonet] Union positions? I am a lab of 5 histotechs and no union. I would never accept a position that is union! --- On Fri, 2/17/12, Pam Bakken wrote: From: Pam Bakken Subject: [Histonet] Union positions? To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 10:53 AM Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? Thanks in advance for your help! Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Fri Feb 17 11:32:58 2012 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Feb 17 11:33:07 2012 Subject: [Histonet] Ventana XT Closed System misnomer In-Reply-To: References: Message-ID: Eric, I wouldn't say my mind is closed on the issue and I appreciate your thoughts and opinions. I agree wholeheartedly about the advantages of the XT and to say that the Benchmark XT is a closed system is simply to state that it is more "closed" regarding reagents than some systems (open) where the reagents are purchsed from various vendors in whatever form you choose. Some people who use all non-Ventana antibodies may find this as a "con" vs. a "pro" when it comes to considering purchasing an XT for their lab. Thanks. Christie Gowan HT(ASCP) > Date: Thu, 16 Feb 2012 15:41:35 -0500 > From: gagnone@KGH.KARI.NET > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ventana XT Closed System misnomer > > Responding to Del who asked about pros/cons of Ventana BenchMark XT: > > > > Ventana is often equated with 'closed system'. This is a misnomer. The > only thing I regard as closed (besides some colleagues' minds on this > issue) is the detection system kit and bulk reagents that are used on > the instrument. While these are proprietary, they are also of extremely > high quality, resiliency, consistency, and in my opinion, dependability. > Preparing one's own reagents can lead to a lack of consistency of > staining, and we have confidence in Ventana reagents. > > > > As others have correctly noted...other non-Ventana antibodies can be > used in Ventana dispensers. We use an equal mix of ready-to-use > antibodies with our own diluted antibodies from other suppliers. How is > this a closed system? > > > > Pre-treatments can also be used in Ventana dispensers. There is a range > of non-ab reagents such as hematoxylins and proteases. There are a > range of detection system kits as well - at least four that I can think > of. > > > > Perhaps this sounds like my personal crusade, as a Ventana user; to try > to demolish the 'closed system' myth. In my professional interactions > with non-Ventana users, this is often heard as a derogatory, negative > reaction to Ventana, which I certainly speak truth to at every > opportunity. > > > > Del, we have found the BenchMark XT's very reliable, with good support > and service, plus excellent quality reagents including antibodies. > > > > Eric Gagnon MLT > > Histology Laboratory > > Kingston General Hospital, > > Kingson, Ontario, Canada > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Fri Feb 17 12:53:02 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Fri Feb 17 12:53:09 2012 Subject: [Histonet] Cell Block Message-ID: Happy Friday Histonetters, We have a pathologist that will be ordering all the latest test such as MMR - ALK Mutation and all of the other good ones that oncologist will be relying on to treat their patients. He wants to order these test on cell block material that he gets from FNA's. As is we get scant material from FNA's for cell block preparation. Question is: What are other facilities doing to maximize their yield on cell blocks? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From jmcgough <@t> clinlab.com Fri Feb 17 13:09:06 2012 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Fri Feb 17 13:09:13 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: Message-ID: We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From rjbuesa <@t> yahoo.com Fri Feb 17 13:20:04 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 17 13:20:09 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: Message-ID: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides.?Adequate humidity is required to be controlled?during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J.? --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmcgough <@t> clinlab.com Fri Feb 17 13:24:13 2012 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Fri Feb 17 13:24:17 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: We use the Dako Autostainer. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 17, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J. --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 17 13:26:34 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 17 13:26:47 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> References: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides.?Adequate humidity is required to be controlled?during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J.? --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ross <@t> premierlab.com Fri Feb 17 13:32:25 2012 From: ross <@t> premierlab.com (Ross Benik) Date: Fri Feb 17 13:32:28 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: References: , Message-ID: <14E2C6176416974295479C64A11CB9AE011390CDC78C@SBS2K8.premierlab.local> Jason, We've experienced similar problems a few summers ago when the environmental conditions inside the Autostainers (dako) reach high temperatures and low humidity. We keep thermometers inside the stainers to monitor these levels. To remedy this problem, before every IHC run, I add a few damp paper towels in the stainer sink. As the stain progresses, liquid is added to the paper towels by the stainer so I never have to worry about it drying out. Since we've started doing this, the humidity ranges anywhere from 35-55% and an ending temperature of no more than 28C. With these conditions, we've yet to experience slides drying during a staining run. Another factor to consider is volume of reagent added to each slide, we do 100 ul per drop zone (3 drop zones total). The longest a reagent left on a slide is 1 hour max for a few of our primary antibodies. Hope this helps, Ross ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough [jmcgough@clinlab.com] Sent: Friday, February 17, 2012 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels and IHC staining We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCBRITTON <@t> Cheshire-Med.COM Fri Feb 17 13:33:05 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Fri Feb 17 13:33:10 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> References: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: The Bond Max from Leica never has this problem! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J. --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Fri Feb 17 13:39:00 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Feb 17 13:38:55 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> References: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <003f01ccedab$cc196a70$644c3f50$@com> NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides.?Adequate humidity is required to be controlled?during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J.? --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Feb 17 13:51:31 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Feb 17 13:51:36 2012 Subject: [Histonet] annual salary survey Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E1A983D@evcspmbx3.ads.northwestern.edu> Has there been a new salary survey done yet? I have the one from last January's Advance magazine. Anyone seen another one lately? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From shive003 <@t> umn.edu Fri Feb 17 13:56:41 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Feb 17 13:56:46 2012 Subject: Fwd: [Histonet] Humidity levels and IHC staining In-Reply-To: <003f01ccedab$cc196a70$644c3f50$@com> References: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> <003f01ccedab$cc196a70$644c3f50$@com> Message-ID: I've had 3 Dakos and have had the drying phenomenon in all of them to some extent. We put wet paper towels in the stain sink, as others have mentioned. Plus, we found that most of the problem came from having an air gap between the hood and the unit, which wasn't closed entirely by the gasket. (The circulating room air got through the gap and dried out the slides in that front row area.) We use a long piece of styrofoam padding, cut it to length, and insert that between the bottom of the closed hood and the metal base at the beginning of each staining run, and it blocks all unwanted airflow into the slide chamber. Jan Shivers UMN ---------- Forwarded message ---------- From: Cynthia Pyse Date: Fri, Feb 17, 2012 at 1:39 PM Subject: RE: [Histonet] Humidity levels and IHC staining To: "Morken, Timothy" , Rene J Buesa < rjbuesa@yahoo.com>, histonet@lists.utsouthwestern.edu, Jason McGough < jmcgough@clinlab.com> NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J. --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claycal44 <@t> yahoo.com Fri Feb 17 14:16:01 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Fri Feb 17 14:16:07 2012 Subject: Fwd: [Histonet] Humidity levels and IHC staining In-Reply-To: References: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com> <8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> <003f01ccedab$cc196a70$644c3f50$@com> Message-ID: <1329509761.54353.YahooMailNeo@web164504.mail.gq1.yahoo.com> We have the Lab Vision 360 from Thermo Fisher and we have really bad problems with the slides drying out.? We put wet paper towels and water boats in, but after 20 or 30 minutes, the slides have very little liquid on them.? I have gone back a lot to manual staining except for stains that work at 30 minutes.? By the way, we are in Ca.? Has anyone else that has this instrument have the same problem? Nancy From: Jan Shivers To: histonet Sent: Friday, February 17, 2012 11:56 AM Subject: Fwd: [Histonet] Humidity levels and IHC staining I've had 3 Dakos and have had the drying phenomenon in all of them to some extent.? We put wet paper towels in the stain sink, as others have mentioned.? Plus, we found that most of the problem came from having an air gap between the hood and the unit, which wasn't closed entirely by the gasket.? (The circulating room air got through the gap and dried out the slides in that front row area.)? We use a long piece of styrofoam padding, cut it to length, and insert that between the bottom of the closed hood and the metal base at the beginning of each staining run, and it blocks all unwanted airflow into the slide chamber. Jan Shivers UMN ---------- Forwarded message ---------- From: Cynthia Pyse Date: Fri, Feb 17, 2012 at 1:39 PM Subject: RE: [Histonet] Humidity levels and IHC staining To: "Morken, Timothy" , Rene J Buesa < rjbuesa@yahoo.com>, histonet@lists.utsouthwestern.edu, Jason McGough < jmcgough@clinlab.com> NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J. --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Fri Feb 17 15:17:40 2012 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Feb 17 15:17:44 2012 Subject: [Histonet] Union positions? In-Reply-To: References: Message-ID: Pam, I believe only the city morgue on Oahu have the histotech-type positions in Hawaii that are union. There are about 48 histotechs or histotech-like postions in 14 centers across 4 of 8 Hawaiian islands (the other 4 islands are too small to support full medical facilities-and one is still under water). We have federal, state, city/county and Kaiser Permanante, positions that most think of being unionized. Most of us were offered to join, but union tactics and dues were not really what we got into health care for. Hugh Luk, HTL (ASCP) UH Cancer Center- PSR lab manager Honolulu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Bakken > Sent: Friday, February 17, 2012 10:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Union positions? > > Trying to put together some numbers, any and all responses to these questions would be greatly appreciated. How many HT's are in union positions? If you were applying for a position, how much of a factor would it be if it was a union position? > > Thanks in advance for your help! > > Confidentiality Statement: > This email/fax, including attachments, may include confidential > and/or proprietary information and may be used only by the > person or entity to which it is addressed. If the reader of > this email/fax is not the intended recipient or his or her > agent, the reader is hereby notified that any dissemination, > distribution or copying of this email/fax is prohibited. If you > have received this email/fax in error, please notify the sender > by replying to this message and deleting this email or > destroying this facsimile immediately. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpheneger <@t> OSIP.com Fri Feb 17 15:54:45 2012 From: tpheneger <@t> OSIP.com (Pheneger, Tracy) Date: Fri Feb 17 15:54:51 2012 Subject: [Histonet] Humidity levels and IHC staining In-Reply-To: <003f01ccedab$cc196a70$644c3f50$@com> References: <1329506404.14877.YahooMailClassic@web162102.mail.bf1.yahoo.com><8D7C2D242DBD45498006B21122072BF89F5EE7F0@MCINFRWEM003.ucsfmedicalcenter.org> <003f01ccedab$cc196a70$644c3f50$@com> Message-ID: <1F8A6EF2842BF5429D1487DA39FEE23F0A1E98@bo-wexmb01.osip.com> Hi; We had the same problem, but are in the dry state of Colorado. I have a couple of suggestions. Make sure that the seal on your lid is adequate, as overtime they can rot. Also, make sure that your racks are not warped. Warped racks can cause some of the liquid to leak out of the sides. We also put a shallow pan of water during runs just to humidify the air a bit. Good luck, Tracy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, February 17, 2012 12:39 PM To: 'Morken, Timothy'; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; 'Jason McGough' Subject: RE: [Histonet] Humidity levels and IHC staining NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides.?Adequate humidity is required to be controlled?during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? Ren? J.? --- On Fri, 2/17/12, Jason McGough wrote: From: Jason McGough Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Feb 17 16:01:48 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Feb 17 16:01:54 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 22 In-Reply-To: <4f3e786e.0654650a.49f9.ffffb3d3SMTPIN_ADDED@mx.google.com> References: <4f3e786e.0654650a.49f9.ffffb3d3SMTPIN_ADDED@mx.google.com> Message-ID: Hi, My lab is in a university with a union. I do not find it helpful at all. The union is great at protecting people from the university firing them weather justly or not. It keeps all salaries the same weather one tech works harder than another or not. It prevents techs from getting a job based on their skills. Salaries are uniform and not merit based. Annual reviews are not even merit based. I really feel that I can make a better argument for my compensation on my own without having to negotiate for the whole university. If a tech gets a better offer elsewhere the department can't counter-offer for the tech without the whole university expecting the same. Why stay? I really enjoy the work I am doing and there are certain benefits that I know I can't get elsewhere. Is that because of the union? Perhaps to some extent, but these benefits existed before the union started. If there were no union and there was a vote to have one start up, I would vote NO. I don't expect everyone would agree with me, but that is my observation. Amos On Fri, Feb 17, 2012 at 10:55 AM, wrote: > Message: 21 > Date: Fri, 17 Feb 2012 09:53:50 -0600 > From: "Pam Bakken" > Subject: [Histonet] Union positions? > To: > Message-ID: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> > Content-Type: text/plain; charset="us-ascii" > > Trying to put together some numbers, any and all responses to these > questions would be greatly appreciated. How many HT's are in union > positions? If you were applying for a position, how much of a factor would > it be if it was a union position? > > Thanks in advance for your help! > From mamawooo <@t> hotmail.com Fri Feb 17 19:32:24 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Feb 17 19:32:30 2012 Subject: [Histonet] Ventana xt In-Reply-To: References: <002801ccecd8$f18aac70$d4a00550$@vetmed.lsu.edu>, , Message-ID: Thanks for correcting me.Jan > Subject: RE: [Histonet] Ventana xt > Date: Fri, 17 Feb 2012 08:23:33 -0600 > From: LSebree@uwhealth.org > To: mamawooo@hotmail.com; christiegowan@msn.com; dphillips@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu > > Side note: only the Ventana Ultra allows adding slides as you go, not > the XT. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice > Mahoney > Sent: Friday, February 17, 2012 7:52 AM > To: Christie Gowan; dphillips@vetmed.lsu.edu; histonet > Subject: RE: [Histonet] Ventana xt > > > I think the Ventana XT is the best instrument for adding slides as you > go which makes it perfect for a LEAN lab. This will reduce your TAT.The > instrument is so easy to use it is virtually impossible to mess up with > it's bar coding and visual control. Very user friendly. > Jan Mahoney,Omaha, NE > > From: christiegowan@msn.com > > To: dphillips@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu > > Date: Thu, 16 Feb 2012 19:46:35 +0000 > > Subject: RE: [Histonet] Ventana xt > > CC: > > > > > > Pros: > > Lends itself well to labs that do basic IHC staining Antibodies are > > pre-diluted so no guess work on dilutions Good for batching runs > > Consistent quality of slides > > 24 hour technical support > > Can run molecular probes > > protocols are easy to adjust > > > > Cons: > > Closed system > > Must use Ventana antibodies or purchase special dispensers if using > > non Ventana products Pre-dilute antibodies are pricey Stand alone > > instrument so must have space for it > > > > I'm sure there is more but you just really need to see what your needs > are. We have the XT and Ultra as well as open systems. We love the > Ventana's but we also will always have open platforms because we do a > lot of research. The work flow is another thing you need to look at. How > many slides do you turn out in one day? What is your turn around time? > The XT is a great instrument but it depends on your lab. Hope this > helps. > > Christie Gowan > > UAB Hospital > > > > > > > > > From: dphillips@vetmed.lsu.edu > > > To: histonet@lists.utsouthwestern.edu > > > Date: Thu, 16 Feb 2012 12:29:37 -0600 > > > Subject: [Histonet] Ventana xt > > > > > > Looking for any pros and cons on the Ventana XT. > > > > > > > > > > > > Thanks > > > > > > Del > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Feb 18 08:20:32 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Feb 18 08:20:44 2012 Subject: [Histonet] Cell Block In-Reply-To: References: Message-ID: <2ABDCE19-A59D-428C-9227-3B7EA9C9E2CC@yahoo.com> A couple of things i did when i had this same issue at one time was If the fna had teeny core biopsies we would separate into two blocks. This allowed for one block to get all the usual in house antibodies and the other could be sent out for special testing. They both got H&E s. another thing is if we suspected cancer from looking at the clinical info we would skip doing cytospins and put everything in for cell block. These methods are not 100% but these two things implemented significantly decreased our need to ever be put on the spot of not having enough biopsy or for the pathologist to have to ask for a rebiopsy. Oh another thing if you only have a cell block you can devide it into 2 blocks if you have visual amounts of cells us my opinion. I've also found that proper cell block preparation is key. You'd be surprised at how many times I've seen people adding way to much cell block material to a specimen and dispersing the cells too much. Anyway. That's my two cents. Happy weekend! Kim D Sent from my iPhone On Feb 17, 2012, at 1:53 PM, Amy Self wrote: > Happy Friday Histonetters, > > We have a pathologist that will be ordering all the latest test such as MMR - ALK Mutation and all of the other good ones that oncologist will be relying on to treat their patients. He wants to order these test on cell block material that he gets from FNA's. As is we get scant material from FNA's for cell block preparation. > > Question is: What are other facilities doing to maximize their yield on cell blocks? > > Thanks in advance for your help, > Amy > > > Amy Self > Georgetown Hospital System > 843-527-7179 > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peggybrask1 <@t> gmail.com Sat Feb 18 11:19:38 2012 From: peggybrask1 <@t> gmail.com (peggy brask) Date: Sat Feb 18 11:19:43 2012 Subject: [Histonet] Looking for casual Histotech position in Minnesota Message-ID: Hello, My name is Peggy Brask and I am looking for a casual or permanent part-time histotech position in Minnesota. My schedule is extremely flexible. I could work 2-3 days per week or just fill in as needed during vacations or leaves etc. I don't need added benefits such as insurance. From tammy <@t> jit-labs.com Sat Feb 18 12:19:22 2012 From: tammy <@t> jit-labs.com (Tammy Schwalb) Date: Sat Feb 18 12:19:32 2012 Subject: [Histonet] (no subject) Message-ID: <6FA9EA82-C076-487B-A0B1-BC630248D1C5@jit-labs.com> Sent from my iPhone From rboen <@t> clearwire.net Sat Feb 18 13:19:09 2012 From: rboen <@t> clearwire.net (Rick and Sue Boen) Date: Sat Feb 18 13:19:26 2012 Subject: [Histonet] RE: Humidity Levels and IHC Staining Message-ID: Jason, We had 2 DAKO Autostainers before moving up to the Autostainer Link 48. I can't remember what the Autostainers were like, but the Links both had a gap between the metal frame and plastic covers (not the movable lids but the solid mounted plastic. We experienced drying issues during the winter season that even lots of water dishes in the chamber could not overcome. We had a service tech come in work on the problem. He consulted with DAKO engineers they came up with a solution to the problem. The next day, he came in with a roll of black duct tape and taped up all the gaps. Not exactly an aesthetic fix to the problem, especially given the cost of each instrument, but it did solve the drying problem. We were also told of other things to look for, such as instrument leveling, waste sink leveling and warped racks. If you're using the PT retrieval modules, for sure check the racks as the repeated heating/cooling cycles of retrieval makes periodic rack replacement a necessity. From ree3 <@t> leicester.ac.uk Mon Feb 20 04:19:38 2012 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Feb 20 04:19:53 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 22 In-Reply-To: References: <4f3e786e.0654650a.49f9.ffffb3d3SMTPIN_ADDED@mx.google.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F769583F@EXC-MBX3.cfs.le.ac.uk> I have been a Trade Union(TU) member for all my working life, primarily because I believed in the TU concept of workers coming together to further their goals and as a counterbalance against exploitative employers, latterly I view TU membership more as an insurance policy should I need it against my employer, somewhere to get advice and legal help from should it become necessary.The trouble with merit based salaries is the subjectiveness of their assessments, for example if an employee has a personality clash or whatever with his line manager then he is unlikely to be correctly awarded financially. Richard Edwards Leicester U.K. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: 17 February 2012 22:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 22 Hi, My lab is in a university with a union. I do not find it helpful at all. The union is great at protecting people from the university firing them weather justly or not. It keeps all salaries the same weather one tech works harder than another or not. It prevents techs from getting a job based on their skills. Salaries are uniform and not merit based. Annual reviews are not even merit based. I really feel that I can make a better argument for my compensation on my own without having to negotiate for the whole university. If a tech gets a better offer elsewhere the department can't counter-offer for the tech without the whole university expecting the same. Why stay? I really enjoy the work I am doing and there are certain benefits that I know I can't get elsewhere. Is that because of the union? Perhaps to some extent, but these benefits existed before the union started. If there were no union and there was a vote to have one start up, I would vote NO. I don't expect everyone would agree with me, but that is my observation. Amos On Fri, Feb 17, 2012 at 10:55 AM, wrote: > Message: 21 > Date: Fri, 17 Feb 2012 09:53:50 -0600 > From: "Pam Bakken" > Subject: [Histonet] Union positions? > To: > Message-ID: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> > Content-Type: text/plain; charset="us-ascii" > > Trying to put together some numbers, any and all responses to these > questions would be greatly appreciated. How many HT's are in union > positions? If you were applying for a position, how much of a factor would > it be if it was a union position? > > Thanks in advance for your help! > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Mon Feb 20 08:28:25 2012 From: member <@t> linkedin.com (Peggy Brask via LinkedIn) Date: Mon Feb 20 08:28:31 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <278695017.6948096.1329748105067.JavaMail.app@ela4-app0128.prod> LinkedIn ------------ Peggy Brask requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Peggy Accept invitation from Peggy Brask http://www.linkedin.com/e/yvpgd1-gyvlosiu-3o/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I227808889_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYVe3wUc3wTcz99bRBxhlhGuAxPbPcPdz4Ve3oRejkLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=00s_4VP1IAb581 View invitation from Peggy Brask http://www.linkedin.com/e/yvpgd1-gyvlosiu-3o/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I227808889_13/3cNnPAUe3wMe3sOcAALqnpPbOYWrSlI/svi/?hs=false&tok=2UnlCB0YgAb581 ------------------------------------------ Why might connecting with Peggy Brask be a good idea? Peggy Brask's connections could be useful to you: After accepting Peggy Brask's invitation, check Peggy Brask's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From talulahgosh <@t> gmail.com Mon Feb 20 09:06:44 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Feb 20 09:06:53 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 22 In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A4F769583F@EXC-MBX3.cfs.le.ac.uk> References: <4f3e786e.0654650a.49f9.ffffb3d3SMTPIN_ADDED@mx.google.com> <7722595275A4DD4FA225B92CDBF174A101A4F769583F@EXC-MBX3.cfs.le.ac.uk> Message-ID: I wish I worked for a union because techs are paid very little to do a lot of work (research in academics). We're stuck in a pay range as well, which depends on how much education you've had. If you knew how little I make for what my responsibilities, no one would be taking my job. I do it because I enjoy it, not because it pays well. I don't ever expect it to pay well either. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Mon, Feb 20, 2012 at 5:19 AM, Edwards, Richard E. wrote: > I have been a Trade Union(TU) member for all my working life, primarily > because I believed in the TU concept of workers coming together to further > their goals and as a counterbalance against exploitative employers, > latterly I view TU membership more as an insurance policy should I need > it against my employer, somewhere to get advice and legal help from > should it become necessary.The trouble with merit based salaries is the > subjectiveness of their assessments, for example if an employee has a > personality clash or whatever with his line manager then he is unlikely to > be correctly awarded financially. > > Richard Edwards > > Leicester U.K. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks > Sent: 17 February 2012 22:02 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 22 > > Hi, > My lab is in a university with a union. I do not find it helpful at > all. The union is great at protecting people from the university firing > them weather justly or not. It keeps all salaries the same weather one tech > works harder than another or not. It prevents techs from getting a job > based on their skills. Salaries are uniform and not merit based. Annual > reviews are not even merit based. I really feel that I can make a better > argument for my compensation on my own without having to negotiate for the > whole university. If a tech gets a better offer elsewhere the department > can't counter-offer for the tech without the whole university expecting the > same. > Why stay? I really enjoy the work I am doing and there are certain > benefits that I know I can't get elsewhere. Is that because of the union? > Perhaps to some extent, but these benefits existed before the union > started. If there were no union and there was a vote to have one start up, > I would vote NO. I don't expect everyone would agree with me, but that is > my observation. > > Amos > > On Fri, Feb 17, 2012 at 10:55 AM, < > histonet-request@lists.utsouthwestern.edu > > wrote: > > > Message: 21 > > Date: Fri, 17 Feb 2012 09:53:50 -0600 > > From: "Pam Bakken" > > Subject: [Histonet] Union positions? > > To: > > Message-ID: <4F3E23AE02000000000570D5@vcgwia1.childrenshc.org> > > Content-Type: text/plain; charset="us-ascii" > > > > Trying to put together some numbers, any and all responses to these > > questions would be greatly appreciated. How many HT's are in union > > positions? If you were applying for a position, how much of a factor > would > > it be if it was a union position? > > > > Thanks in advance for your help! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JThawley <@t> ShoreMemorial.org Mon Feb 20 12:11:04 2012 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Mon Feb 20 12:11:13 2012 Subject: [Histonet] Bleach melanin pigment Message-ID: Hello All, I am trying to bleach melanin pigment from sections for IHC and I do not have oxalic acid. Is there anything I can use as a substitute? Thanks in advance! Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. From jclark <@t> pcnm.com Mon Feb 20 12:17:34 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Feb 20 12:17:41 2012 Subject: [Histonet] Pigment in H&E slides Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB56133@S10MAILD001N1.SH10.lan> Hi Histonetters, I was hoping you all could help me identify a strange pigment I have occassionally seen in my H&E slides. It's not a formalin pigment and looks to be in the tissue, but on top at the same time. When you focus up and down on it with the microscope its slightly refractive. It looks more to me like a precipitate really than a pigment. It doesn't happen on all slides just on the odd one which makes it easier to rule out formalin pigment. IF the pH was off on my formalin, all the blocks would have pigment in them. It almost looks to me like talcum powder. I was able to remove it by bleaching it out with potassium permanganate. Any ideas? From Ronald.Houston <@t> nationwidechildrens.org Mon Feb 20 12:35:28 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Feb 20 12:35:41 2012 Subject: [Histonet] CAP GEN.55500 Message-ID: How are people tackling this General Competency question from CAP in Anatomic Pathology Labs, as in the strictest sense (in most cases) results are not being generated by the technical staff? There is dispute between pathologists, techs and administrators about what would define a test system, and how specifically you would monitor the competency. Would appreciate some ammunition........ Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From scbymar <@t> yahoo.com Mon Feb 20 13:48:40 2012 From: scbymar <@t> yahoo.com (MaryAnn Dixon) Date: Mon Feb 20 13:48:44 2012 Subject: [Histonet] looking for employment Message-ID: <1329767320.41465.YahooMailNeo@web30102.mail.mud.yahoo.com> Hello everyone in histoland. I just moved from Florida to Chesapeake, VA and are looking for employment. I am? certified HT and QIHC. Please email me if you know of any employment opportunities.? Thank you. ? MaryAnn From mousecatchr <@t> aol.com Mon Feb 20 14:28:17 2012 From: mousecatchr <@t> aol.com (Mousecatcher) Date: Mon Feb 20 14:28:23 2012 Subject: [Histonet] Emory Adventist Hospital Message-ID: <8CEBE254FF11EA3-C78-57700@Webmail-d106.sysops.aol.com> Does anyone know anything about this hospital or the pathologist in charge of the histology lab? You can contact me off list if you prefer... Thanks From POWELL_SA <@t> mercer.edu Mon Feb 20 15:55:59 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Feb 20 15:56:09 2012 Subject: [Histonet] GSH meeting weekly reminder Message-ID: <9BF995BC0E47744E9673A41486E24EE24AD7F4590F@MERCERMAIL.MercerU.local> Hi Histonetters and fellow histotechs, I am making sure you don't forget about our meeting coming up. The popular workshops are filling up fast. The workshop fee is all inclusive, $100 for a possible six is a great deal. Most individual workshop fees are around $40 each. Don't miss out, earn your CEUs at the Georgia Society for Histotechnology - Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,. The Deadline for receiving the discounted hotel rate is March 13th, so act now. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here in Georgia. Make reservations now at 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program which can be found at http://www.histosearch.com/gsh/symposium.html. Plan to attend, bring the family for a vacation, Callaway Gardens is a wonderful family experience. If you have any questions please contact GSH President - Mike Ayers at lmayers@charter.net, GSH Vice President and Exhibit Liaison - Wanda Simons at wandrous@att.net or myself powell_sa@mercer.edu. Come to Georgia and experience........................................ Histotechnology - Southern Style Shirley Powell GSH Secretary From cforster <@t> umn.edu Mon Feb 20 16:01:34 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Feb 20 16:01:37 2012 Subject: [Histonet] TMA blocks Message-ID: <4F42C2BE.3080901@umn.edu> Hey Histonetters! We have the first generation of the Beecher machine, manual version. We would like to do a super TMA that would hold 180+1.0mm punches. When you do a single TMA the tissue area is 1x1 and out largest mold is 3x3. Has anyone ever trimmed the TMA's down and embedded 2 or 3 of them into one big block? Thanks in advance!! Colleen Forster Bionet Histology Laboratory U of MN, AHC From hfedor <@t> jhmi.edu Mon Feb 20 16:41:25 2012 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Feb 20 16:41:39 2012 Subject: [Histonet] TMA blocks In-Reply-To: <4F42C2BE.3080901@umn.edu> References: <4F42C2BE.3080901@umn.edu> Message-ID: Hello Colleen, We use standard size molds (2.5cm X 3.5cm) that are a litte deeper (7mm) in our core to make the recipient blocks. 2.5cm X 3.5cm. and our TMA with the 1.0mm punch have 13 rows and 16 columns = 208 cores. You can contact me if you want more information. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Biorepository, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Colleen Forster Sent: Monday, February 20, 2012 5:02 PM To: Histonet Subject: [Histonet] TMA blocks Hey Histonetters! We have the first generation of the Beecher machine, manual version. We would like to do a super TMA that would hold 180+1.0mm punches. When you do a single TMA the tissue area is 1x1 and out largest mold is 3x3. Has anyone ever trimmed the TMA's down and embedded 2 or 3 of them into one big block? Thanks in advance!! Colleen Forster Bionet Histology Laboratory U of MN, AHC From daniela.bodemer <@t> mcri.edu.au Mon Feb 20 16:57:44 2012 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Mon Feb 20 16:57:53 2012 Subject: [Histonet] FW: Oil Red O Message-ID: <9DF797D618351549B984596F01A1FE1D0232024B@murmx.mcri.edu.au> From: Daniela Bodemer Sent: Thursday, 16 February 2012 9:59 AM To: Histonet@lists.utsouthwestern.edu Subject: Oil Red O Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading and should they be kept in a folder in the fridge? Thanks for your thoughts, Daniela Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From Diane.Tokugawa <@t> kp.org Mon Feb 20 23:14:19 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Mon Feb 20 23:14:39 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 02/20/2012 and will not return until 02/22/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From JThawley <@t> ShoreMemorial.org Tue Feb 21 08:35:08 2012 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Tue Feb 21 08:35:13 2012 Subject: [Histonet] Histotech Position Message-ID: Hello all, We have a histology position open. Requirements are HT certified or eligible, we are willing to train. Anyone interested please contact me with questions or you can apply at http://shoremedicalcenter.org/careers. Thanks! Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. From PMonfils <@t> Lifespan.org Tue Feb 21 09:48:08 2012 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Feb 21 09:48:14 2012 Subject: [Histonet] Oil Red O In-Reply-To: <9DF797D618351549B984596F01A1FE1D0231FC21@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D0231FC21@murmx.mcri.edu.au> Message-ID: <4EBFF65383B74D49995298C4976D1D5E09434E8A@LSRIEXCH1.lsmaster.lifespan.org> Oil Red O slides will never "fade". The technique isn't like typical histochemical procedures where dye molecules bind chemically to tissue components. In Oil Red O staining there isn't any chemical binding that can be reversed. What you end up with is a solution of the dye in the fat droplets. Once coverslipped with an aqueous mounting medium, the dye cannot escape from the fat droplets because it is insoluble in water. Therefore the dye in the fat droplets is just as stable as the dye solution in the bottle, and the slides will remain good as long as the coverslipping medium doesn't dry out - which in this case is the reason for refrigeration, to slow the drying of the mounting medium. From patjnm <@t> gwumc.edu Tue Feb 21 10:43:55 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Tue Feb 21 10:44:35 2012 Subject: [Histonet] disposable blade system for microm 505e or Message-ID: <4F43837B.DB55.001F.1@gwumc.edu> Hi netters, I have a system that uses full sized microtome blades for the Microm 505E cryostat. I think it would be cheaper to order the item I have seen before that us old timers used when disposable blade systems first came on the seen. The blade holder looks and feels like an old time microtome blade, but upon closer inspection is actually a holder for a disposable blade, then the entire "unit" fits into the microtome like the old blades did. So if I had one of those I could place that in the cryostat instead of using the entire assembly. I would prefer the entire assembly but I am as low budget as they get right now. Everything by hand(including ice bath, hot plates and paraffin dispensers for embedding). That will end soon though. Still hand processing, staining, coverslipping etc though. ANyone out there want to throw anything my way, sure would preeshiate it. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From GauchV <@t> mail.amc.edu Tue Feb 21 11:18:09 2012 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Tue Feb 21 11:18:24 2012 Subject: [Histonet] Processor Question Message-ID: Hi everyone, We are in the market for new processors...and I was wondering if anyone could give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how reliable are they? Ease of use ? Any known problems? Tissues process well? You know....all the usual questions we all ask for new equipment..... Thanks in advance for your help, Vicki Gauch AMCH Albany, NY From LTurner1 <@t> seton.org Tue Feb 21 11:26:10 2012 From: LTurner1 <@t> seton.org (Turner, Leandra) Date: Tue Feb 21 11:26:18 2012 Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> Hello Everyone, I am trying to find out a few of things about making cell pellets on cerebrospinal fluids. I would like to know: 1. If anyone has ever made cell pellets from CSF's using Histogel and has any tips or procedures they could share? 2. How to process the CSF pellets made with Histogel, do we need a routine or stat process? (we use a Sakura Tissue Tek) 3. Can you do IHC staining on the pellets? Thank you for any and all help in advance. Leandra Turner, HT (ASPC)CM From darcyb <@t> slonepartners.com Tue Feb 21 12:08:28 2012 From: darcyb <@t> slonepartners.com (Darcy Bloch) Date: Tue Feb 21 12:08:36 2012 Subject: [Histonet] Seeking a Histology Supervisor in Connecticut Message-ID: Slone Partners seeks a Histology Supervisor for our hospital based laboratory The successful candidate redesign and be great at managing change. people in the department, including 2 supervisors. experience is a plus. Qualified certification, with high-volume laboratory. Special features of this position: have the opportunity to help redesign this If you meet these qualifications for this position, please submit your resume darcyb@slonepartners.com. If you wish to be considered Tara Kochis at tara@slonepartners.com. All inquiries are kept confidential. From pathlocums <@t> gmail.com Tue Feb 21 12:33:27 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Feb 21 12:33:35 2012 Subject: [Histonet] Processor Question Message-ID: <-2296715788321707800@unknownmsgid> Design flaw in the screen display. It is in the way of the chamber when opening chamber. If your not careful you will break the screen. Happens fairly often. Sent from my Windows Phone From: Gauch, Vicki Sent: 2/21/2012 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Question Hi everyone, We are in the market for new processors...and I was wondering if anyone could give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how reliable are they? Ease of use ? Any known problems? Tissues process well? You know....all the usual questions we all ask for new equipment..... Thanks in advance for your help, Vicki Gauch AMCH Albany, NY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Tue Feb 21 14:23:07 2012 From: plucas <@t> biopath.org (Paula Lucas) Date: Tue Feb 21 14:21:10 2012 Subject: [Histonet] Job Opening in Orange county California Message-ID: A part-time histotech position is open in our histology laboratory. Tuesday through Saturday starting at 5 am. If anyone is interested, please either fax or email me your resume. Thank you, Paula Lucas Bio-Path Medical Group Fountain Valley, CA 92708 Fax: 714-755-2984 From amosbrooks <@t> gmail.com Tue Feb 21 16:22:57 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Feb 21 16:23:05 2012 Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Message-ID: Hi, I haven't done a cellblock on CSF with Histogel, but I have has success with some fairly scanty cell culture specimens. A short processing cycle would be best. Try to make sure you have removed as much supernatant as possible to keep the gel from shriveling during processing. The IHC will be fine as long as the cells are suitably fixed ahead of time. Here is a link to the packaging insert for Histogel which describes preparation of cell blocks. https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf And here's a video of people using it for cytology specimens. http://tinyurl.com/7jb522e Amos On Tue, Feb 21, 2012 at 1:01 PM, wrote: > Message: 15 > Date: Tue, 21 Feb 2012 11:26:10 -0600 > From: "Turner, Leandra" > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: > Message-ID: > <3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> > Content-Type: text/plain; charset="us-ascii" > > > > > > Hello Everyone, > > I am trying to find out a few of things about making cell pellets on > cerebrospinal fluids. I would like to know: > > > > 1. If anyone has ever made cell pellets from CSF's using Histogel > and has any tips or procedures they could share? > > > > 2. How to process the CSF pellets made with Histogel, do we need a > routine or stat process? (we use a Sakura Tissue Tek) > > > > 3. Can you do IHC staining on the pellets? > > > > > > Thank you for any and all help in advance. > > > > Leandra Turner, HT (ASPC)CM > From histotech411 <@t> gmail.com Tue Feb 21 21:17:58 2012 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Tue Feb 21 21:18:05 2012 Subject: [Histonet] Does xylene cause skin cancer? Message-ID: I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. From settembr <@t> umdnj.edu Wed Feb 22 05:47:20 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Wed Feb 22 05:47:33 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: References: Message-ID: I would be worried too. Wearing gloves in the lab is always a good practice and I believe, a requirement in my lab whenever handling reagents or the possibility of coming into contact with reagents. You have the right to wear gloves if you want. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 10:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dennis.Hahn <@t> cookchildrens.org Wed Feb 22 06:20:02 2012 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Wed Feb 22 06:39:15 2012 Subject: [Histonet] Storage of Frozen Tissues Message-ID: How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? Thanks, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6168 ________________________________ ---------------------------------------------------------------------------------------- Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --------------------------------------------------------------------------------------- From rjbuesa <@t> yahoo.com Wed Feb 22 07:08:51 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 22 07:08:59 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: Message-ID: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. Ren? J. --- On Tue, 2/21/12, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d.a.faichney <@t> stir.ac.uk Wed Feb 22 07:15:28 2012 From: d.a.faichney <@t> stir.ac.uk (Debbie Faichney) Date: Wed Feb 22 07:15:56 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> References: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> Message-ID: <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. Ren? J. --- On Tue, 2/21/12, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. From rjbuesa <@t> yahoo.com Wed Feb 22 07:16:27 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 22 07:16:35 2012 Subject: [Histonet] Storage of Frozen Tissues In-Reply-To: Message-ID: <1329916587.26606.YahooMailClassic@web162104.mail.bf1.yahoo.com> Tissues in my tumor bank (-80?C) were kept in small (1inchx2inches) labeled zip-lock bags. I do not understand about your concern regarding "tissues being exposed to staff". Inside the bags I used, the tissues were not exposed. If they were taken out for any study or procedures, universal precautions were taken. Ren? J. --- On Wed, 2/22/12, Dennis Hahn wrote: From: Dennis Hahn Subject: [Histonet] Storage of Frozen Tissues To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, February 22, 2012, 7:20 AM How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? Thanks, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6168 ________________________________ ---------------------------------------------------------------------------------------- Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --------------------------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Feb 22 07:21:47 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Feb 22 07:21:56 2012 Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel In-Reply-To: <3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> References: <3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> Message-ID: <1329916907.78300.YahooMailNeo@web130104.mail.mud.yahoo.com> I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material.? Here is my procedure: 1.???? you need about 5X10^ cells per pellet 2.???? spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube 3.???? aspirate and resuspend in 15ml NBF and fix overnight @ RT 4.???? spin @ 2000 rpm for 5 minutes and aspirate the NBF 5.???? microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving) 6.???? histogel should now be at liquid for and about 60C 7.???? resuspend cell pellet in about 350ul of histogel 8.???? place cell pellet in freezer or on ice for about 20 minutes to solidify 9.???? using spatula, remove histogel pellet and place into cassette 10.????????????????? post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed 11.????????????????? process as usual Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Turner, Leandra" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 21, 2012 12:26 PM Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Hello Everyone, ? ? I am trying to find out a few of things about making cell pellets on cerebrospinal fluids.? I would like to know: ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel and has any tips or procedures they could share? ? ? 2. How to process the CSF pellets made with Histogel, do we need a routine or stat process?? (we use a Sakura Tissue Tek) ? ? 3. Can you do IHC staining on the pellets? Thank you for any and all help in advance. Leandra Turner, HT (ASPC)CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Feb 22 07:23:15 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Feb 22 07:23:23 2012 Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel In-Reply-To: <1329916907.78300.YahooMailNeo@web130104.mail.mud.yahoo.com> References: <3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> <1329916907.78300.YahooMailNeo@web130104.mail.mud.yahoo.com> Message-ID: <1329916995.72580.YahooMailNeo@web130102.mail.mud.yahoo.com> I forgot to mention, we prepare these specifically for IHC; so yes, you can do IHC on them. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Kim Merriam To: "Turner, Leandra" ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 22, 2012 8:21 AM Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material.? Here is my procedure: 1.???? you need about 5X10^ cells per pellet 2.???? spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube 3.???? aspirate and resuspend in 15ml NBF and fix overnight @ RT 4.???? spin @ 2000 rpm for 5 minutes and aspirate the NBF 5.???? microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving) 6.???? histogel should now be at liquid for and about 60C 7.???? resuspend cell pellet in about 350ul of histogel 8.???? place cell pellet in freezer or on ice for about 20 minutes to solidify 9.???? using spatula, remove histogel pellet and place into cassette 10.????????????????? post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed 11.????????????????? process as usual Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Turner, Leandra" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 21, 2012 12:26 PM Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Hello Everyone, ? ? I am trying to find out a few of things about making cell pellets on cerebrospinal fluids.? I would like to know: ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel and has any tips or procedures they could share? ? ? 2. How to process the CSF pellets made with Histogel, do we need a routine or stat process?? (we use a Sakura Tissue Tek) ? ? 3. Can you do IHC staining on the pellets? Thank you for any and all help in advance. Leandra Turner, HT (ASPC)CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hsmith <@t> wakehealth.edu Wed Feb 22 08:51:31 2012 From: hsmith <@t> wakehealth.edu (Hilary Smith) Date: Wed Feb 22 08:51:45 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> References: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> Message-ID: You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: "The substance may be toxic to blood, kidneys, liver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage." I would definitely use gloves if I were you. Hilary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney Sent: Wednesday, February 22, 2012 8:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. Ren? J. --- On Tue, 2/21/12, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Wed Feb 22 09:00:59 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed Feb 22 09:01:11 2012 Subject: [Histonet] Histotech & Histology Supervisor Job in Naples, FL Message-ID: Allied Search Partners has been retained for the following searches in Florida. Please forward this along to anyone who you know that would be interested in any of the following positions. We do offer a referral bonus. Please email a copy of updated resume to melissa@alliedsearchpartners.com for a full job description. We have the following positions available: Histotech LOCATION: Naples, FL DEPARTMENT & SCHEDULE: Monday-Friday Day Shift/Full Time (6:30am-2:30pm) Histology Supervisor LOCATION: Naples, FL DEPARTMENT & SCHEDULE: *Bachelor?s Degree Required, FL Histology Supervisor License Eligible Monday-Friday Day Shift/Full Time -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From amber.mckenzie <@t> gastrodocs.net Wed Feb 22 09:26:43 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Feb 22 09:25:03 2012 Subject: [Histonet] Temp verifier slides - Ventana equipment In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. From TGoins <@t> mt.gov Wed Feb 22 11:14:40 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Feb 22 11:14:54 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: References: Message-ID: Nitrile gloves are recommended for all the chemicals we use in our lab except acetone - for acetone latex is recommended. We also coverslip by hand but we wear nitrile gloves without exception. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 8:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Feb 22 12:18:05 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Feb 22 12:18:12 2012 Subject: [Histonet] questions Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From rsrichmond <@t> gmail.com Wed Feb 22 12:48:11 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 22 12:48:15 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? Message-ID: I don't know of any evidence that xylene causes skin cancer. Concern is with absorption through the skin. The most likely problem is with the bone marrow - leukemia and related diseases - from aromatic hydrocarbons (xylene, toluene, benzene) - which of course are present in resinous mounting media even in "xylene free" laboratories. Latex gloves dissolve rapidly. Nitrile rubber is more resistant, though not very. I don't know about vinyl examination gloves. I don't wear gloves in this situation, but obviously a pathologist gets much less exposure than a histotechnologist does. I certainly wouldn't argue with anyone who wanted to wear them. Bob Richmond Samurai Pathologist Knoxville TN From mcauliff <@t> umdnj.edu Wed Feb 22 12:59:35 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Feb 22 12:58:30 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: References: Message-ID: <4F453B17.9030604@umdnj.edu> Hi Jenny: Xylene can pass through the skin via the lipids that surround the skin cells, just the way the medicine in nicotine patches, sea sickness patches and birth control patches can. How much can enter your system is not known to me but you should wear resistant gloves, maybe even double glove. Not wearing the appropriate glove is almost certainly a safety violation. Geoff On 2/21/2012 10:17 PM, Jenny Vega wrote: > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of latex in > the back of the slides. I really don't feel comfortable mounting without > gloves because I heard that xyelene can cause cancer. Some people I know > personally has told me that this is not possible, but I read in some places > that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and > I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From darcyb <@t> slonepartners.com Wed Feb 22 13:22:54 2012 From: darcyb <@t> slonepartners.com (Darcy Bloch) Date: Wed Feb 22 13:22:59 2012 Subject: [Histonet] Seeking a Histology Supervisor in Connecticut Message-ID: <20120222122254.d7277bfda5f067ef1125500b0caaecf8.38a5f72630.wbe@email14.secureserver.net> Slone Partners seeks a Histology Supervisor for our hospital based laboratory client in Connecticut. The successful candidate will have knowledge and ability to operate microtomes manual and automated; operate tissue processors, automated stainer, coverslipper, slide/cassette write; to embed tissues of various sizes and orientations; and to perform special stains both manual and automated. Must be a team player, responsible, proactive, goal and people oriented, focused, punctual and open to learning new tasks. Qualified candidates will have either HT/HTL certification, with supervisory experience, preferably in a busy, high-volume laboratory. Special features of this position: The histology supervisor will have5 years experience in a clinical laboratory with emphasis in anatomic pathology/histology. If you meet these qualifications and would like to be considered for this position, please submit your resume to Darcy Bloch at darcyb@slonepartners.com. If you have experience in the diagnostic laboratory industry and wish to be considered for other roles, please forward your resume to Tara Kochis at tara@slonepartners.com. All inquiries are kept confidential. From Lisa.White3 <@t> va.gov Wed Feb 22 13:23:01 2012 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Wed Feb 22 13:23:31 2012 Subject: [Histonet] RE: Does xylene cause skin cancer? Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397609514952@VHAV09MSGA3.v09.med.va.gov> We use nitrile to coverslip at our lab. Several of us including myself have latex allergy as well as the latex breaks down quickly. Most labs require you to use gloves handling any chemical substance. I have one tech who has years of experience that will suffer greatly at 1 drop of xylene or xylene substitute. With one drop the skin will be eaten away for quite a long time. So nitrile all the way! I don't know about xylene causing skin cancer but heard an old wives tale once that a HT had gotten liver cancer from exposure to xylene. Who knows if it is true? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From brian <@t> prometheushealthcare.com Wed Feb 22 14:35:48 2012 From: brian <@t> prometheushealthcare.com (Brian-Prometheus) Date: Wed Feb 22 14:35:55 2012 Subject: [Histonet] Thank You to all histology professionals! Message-ID: <048401ccf1a1$9160f160$b422d420$@com> Histotechnology Professionals Day is coming up soon! March 10, 2012 Histotechnology Professionals Day is an event designed to bring public awareness and recognition to histotechnology practitioners around the world. It offers an opportunity to recognize and appreciate the individuals who have dedicated themselves to quality patient care. Thank You to all histology professionals! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From SAllen <@t> dsmanitoba.ca Wed Feb 22 15:00:14 2012 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Wed Feb 22 15:01:21 2012 Subject: FW: Subject: Re: [Histonet] Storage of Frozen Tissues Message-ID: Hi, We freeze & store muscle bx's & brain tissue at -70?C since the neuro lab was started (around 1980). We started using cryogenic containers after we found that over time the tissue would dry out with any other type of storage & we have tried them all. Hope this helps, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From histonet.nospam <@t> vneubert.com Wed Feb 22 15:30:24 2012 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Feb 22 15:30:37 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> References: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> Message-ID: <4F455E70.9050409@vneubert.com> Debbie: Are the 30 min official? Any standard procedure that states 30 min as maximum? > If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. > > Debbie Faichney > Institute of Aquaculture > University of Stirling > UK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: 22 February 2012 13:09 > To: histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: Re: [Histonet] Does xylene cause skin cancer? > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of latex in > the back of the slides. I really don't feel comfortable mounting without > gloves because I heard that xyelene can cause cancer. Some people I know > personally has told me that this is not possible, but I read in some places > that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and > I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From DSiena <@t> statlab.com Wed Feb 22 17:35:54 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Feb 22 17:35:58 2012 Subject: [Histonet] RE: questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> Message-ID: Hi Dorothy, I have used reagent alcohol for years both with staining and tissue processing. I have never noticed any artifacts or crystals due to use of reagent alcohol. One thing that you should know is that all denatured alcohols are not the same so make sure that you buy a denatured (reagent) alcohol of good quality. As far as keeping slides unstained forever, the one thing that you need to take into consideration is that if the slide is charged or silane coated that the positive charges (adhesiveness) of the slide will change over time and the sections may fall off during staining. Also the antigenicity of some antibodies decreases over time so if you were to do IHC staining, your results may not be as strong as they would be on a more recently cut slide. Best wishes, Debbie Siena HT (ASCP) QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010? x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, February 22, 2012 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Feb 22 17:51:17 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Feb 22 17:51:24 2012 Subject: [Histonet] questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> Message-ID: <14E15F6D-B3B9-469E-9B1E-EB03324D718D@yahoo.com> Dorothy, saving cut slides forever is not a good idea in my opinion. They attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen specificity. Cut slides is not the answer to me. I had to deal with this a couple if times hopefully your pathologist will understand it's best to leave the cells in the block till need. Now I will say on certain protocols we would take sections and stain them but really need to get out of habit of cutting a bunch of extras that even if you did need would be sub optimal material because of what we just spoke of. Keep it in the block as much as you can. Just my two cents. Kim D Sent from my iPhonem On Feb 22, 2012, at 1:18 PM, "Webb, Dorothy L" wrote: > For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? > > How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block. > > Appreciate ahead of time your responses! > > Dorothy Webb, HT (ASCP) > Regions Hospital > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Bromley <@t> mps.com.au Wed Feb 22 18:06:30 2012 From: Mark.Bromley <@t> mps.com.au (Mark Bromley) Date: Wed Feb 22 18:07:49 2012 Subject: [Histonet] questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43016BE1735489@HPEMX3.HealthPartners.int> Message-ID: <81F34C5B11F00B4F92A723A2F159167703DD492A@SIGCOLNT60.sonichealth.com.au> Hi Dorothy If you want to prolong the viability of pre-cut unstained slides, firstly dry them, then dunk the slide into a bath of molten paraffin wax deep enough to submerge all of the tissue section, then remove it and allow the wax coating to solidify. This will keep the environment out of your cut section. When you need to use the slides, simply heat to melt the excess wax and then dewax and rehydrate in the usual manner. Regards, Mark Bromley. Manager, Histopathology. ___________________________________________ Melbourne Pathology. 103 Victoria Pde, Melbourne. | (03) 92877806 www.mps.com.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 23 February 2012 5:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Feb 22 18:42:59 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Feb 22 18:43:24 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A1C5BA@xmdb02.nch.kids> Hi all, My 2 cents worth, just be careful which gloves you wear. The reason for latex residues appearing on the back of the slides is that you are wearing the wrong gloves. Nitrile gloves are what you need to be using, latex will dissolve in the xylene. Remember (1): Disposable gloves provide a barrier protection when working with small amounts of laboratory chemicals. If a disposable glove becomes contaminated, remove immediately and replace with a new glove. Never reuse disposable gloves. (1) "Guidelines for the selection of Chemically Resistant Gloves" (http://www.bioc.cam.ac.uk/safety/glove_guide.pdf) (2) "Ansell Chemical Resistance Guide" (http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) http://www.aps.anl.gov/Safety_and_Training/User_Safety/gloveselection.html Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Wednesday, 22 February 2012 10:47 PM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Does xylene cause skin cancer? I would be worried too. Wearing gloves in the lab is always a good practice and I believe, a requirement in my lab whenever handling reagents or the possibility of coming into contact with reagents. You have the right to wear gloves if you want. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Tuesday, February 21, 2012 10:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does xylene cause skin cancer? I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Feb 22 18:56:37 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Feb 22 18:57:02 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: References: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A1C5FE@xmdb02.nch.kids> Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, whereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilary Smith Sent: Thursday, 23 February 2012 1:52 AM To: Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: "The substance may be toxic to blood, kidneys, liver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage." I would definitely use gloves if I were you. Hilary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney Sent: Wednesday, February 22, 2012 8:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. Ren? J. --- On Tue, 2/21/12, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From madeleinehuey <@t> gmail.com Wed Feb 22 20:21:54 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Wed Feb 22 20:22:01 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 In-Reply-To: <4f452d63.89c8ec0a.36be.6db9SMTPIN_ADDED@mx.google.com> References: <4f452d63.89c8ec0a.36be.6db9SMTPIN_ADDED@mx.google.com> Message-ID: Message: 15 Date: Wed, 22 Feb 2012 15:26:43 +0000 From: Amber McKenzie Subject: [Histonet] Temp verifier slides - Ventana equipment To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. Amber, We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just fine. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Wed, Feb 22, 2012 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) > ? 2. RE: Processor Question (Davide Costanzo) > ? 3. Job Opening in Orange county California (Paula Lucas) > ? 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) > ? 5. Does xylene cause skin cancer? (Jenny Vega) > ? 6. RE: Does xylene cause skin cancer? (Settembre, Dana) > ? 7. Storage of Frozen Tissues (Dennis Hahn) > ? 8. Re: Does xylene cause skin cancer? (Rene J Buesa) > ? 9. RE: Does xylene cause skin cancer? (Debbie Faichney) > ?10. Re: Storage of Frozen Tissues (Rene J Buesa) > ?11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) > ?12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) > ?13. RE: Does xylene cause skin cancer? (Hilary Smith) > ?14. Histotech & Histology Supervisor Job in Naples, FL > ? ? ?(Melissa Phelan) > ?15. Temp verifier slides - Ventana equipment (Amber McKenzie) > ?16. RE: Does xylene cause skin cancer? (Goins, Tresa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: 21 Feb 2012 13:08:28 -0500 > From: "Darcy Bloch" > Subject: [Histonet] Seeking a Histology Supervisor in Connecticut > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > > ? Slone ?Partners ?seeks ?a ?Histology Supervisor for our hospital based > ? laboratory > > > The ?successful ?candidate ? redesign ?and ?be ?great ?at ?managing change. ? people in the department, including 2 supervisors. ? experience is a plus. > > > > Qualified ? certification, ?with ? high-volume laboratory. > > > > Special ?features ?of this position: ? have the opportunity to help redesign this > > > If ?you ?meet these qualifications ? for ?this ?position, ?please ?submit ?your ?resume ? darcyb@slonepartners.com. > > > > If ?you ? wish ?to be considered ? Tara Kochis at tara@slonepartners.com. > > > > All inquiries are kept confidential. > > > ------------------------------ > > Message: 2 > Date: Tue, 21 Feb 2012 10:33:27 -0800 > From: Davide Costanzo > Subject: RE: [Histonet] Processor Question > To: "Gauch, Vicki" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <-2296715788321707800@unknownmsgid> > Content-Type: text/plain; charset=ISO-8859-1 > > Design flaw in the screen display. It is in the way of the chamber when > opening chamber. If your not careful you will break the screen. Happens > fairly often. > > Sent from my Windows Phone > From: Gauch, Vicki > Sent: 2/21/2012 9:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processor Question > Hi everyone, > We are in the market for new processors...and I was wondering if > anyone could give me some pros and cons for the Tissue Tek VIP 6 > tissue processor - how reliable are they? Ease of use ? Any known > problems? ?Tissues process well? ? You know....all the usual questions > we all ask for new equipment..... > > Thanks in advance for your help, > > Vicki Gauch > AMCH > Albany, NY > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 21 Feb 2012 12:23:07 -0800 > From: "Paula Lucas" > Subject: [Histonet] Job Opening in Orange county California > To: > Message-ID: > Content-Type: text/plain; ? ? ? charset="US-ASCII" > > A part-time histotech position is open in our histology laboratory. > > Tuesday through Saturday starting at 5 am. > > If anyone is interested, please either fax or email me your resume. > > Thank you, > > Paula Lucas > > Bio-Path Medical Group > > Fountain Valley, CA 92708 > > Fax: 714-755-2984 > > > > ------------------------------ > > Message: 4 > Date: Tue, 21 Feb 2012 17:22:57 -0500 > From: Amos Brooks > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > ? ? I haven't done a cellblock on CSF with Histogel, but I have has > success with some fairly scanty cell culture specimens. A short processing > cycle would be best. Try to make sure you have removed as much supernatant > as possible to keep the gel from shriveling during processing. The IHC will > be fine as long as the cells are suitably fixed ahead of time. > > Here is a link to the packaging insert for Histogel which describes > preparation of cell blocks. > https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf > > And here's a video of people using it for cytology specimens. > http://tinyurl.com/7jb522e > > Amos > > > On Tue, Feb 21, 2012 at 1:01 PM, > wrote: > >> Message: 15 >> Date: Tue, 21 Feb 2012 11:26:10 -0600 >> From: "Turner, Leandra" >> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel >> To: >> Message-ID: >> ? ? ? ?<3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> >> >> >> >> Hello Everyone, >> >> ? ?I am trying to find out a few of things about making cell pellets on >> cerebrospinal fluids. ?I would like to know: >> >> >> >> ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel >> and has any tips or procedures they could share? >> >> >> >> ? ? 2. How to process the CSF pellets made with Histogel, do we need a >> routine or stat process? ?(we use a Sakura Tissue Tek) >> >> >> >> ? ? 3. Can you do IHC staining on the pellets? >> >> >> >> >> >> Thank you for any and all help in advance. >> >> >> >> Leandra Turner, HT (ASPC)CM >> > > > ------------------------------ > > Message: 5 > Date: Tue, 21 Feb 2012 23:17:58 -0400 > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of latex in > the back of the slides. I really don't feel comfortable mounting without > gloves because I heard that xyelene can cause cancer. Some people I know > personally has told me that this is not possible, but I read in some places > that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and > I am worried. > > > > Thanks. > > > ------------------------------ > > Message: 6 > Date: Wed, 22 Feb 2012 06:47:20 -0500 > From: "Settembre, Dana" > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: 'Jenny Vega' , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > I would be worried too. > Wearing gloves in the lab is always a good practice and > I believe, a requirement in my lab whenever handling reagents or > the possibility of coming into contact with reagents. > You have the right to wear gloves if you want. > > Dana Settembre > University Hospital - UMDNJ > Newark, NJ > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega > Sent: Tuesday, February 21, 2012 10:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Does xylene cause skin cancer? > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of latex in > the back of the slides. I really don't feel comfortable mounting without > gloves because I heard that xyelene can cause cancer. Some people I know > personally has told me that this is not possible, but I read in some places > that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and > I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Wed, 22 Feb 2012 12:20:02 +0000 > From: Dennis Hahn > Subject: [Histonet] Storage of Frozen Tissues > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? > > Thanks, > Dennis > > Dennis Hahn, HT (ASCP) > Histology Lab Supervisor > Cook Children's Medical Center > 801 7th Avenue > Ft. Worth, TX 76104 > (682) 885-6168 > > > > ________________________________ > > ---------------------------------------------------------------------------------------- > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. > > If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > --------------------------------------------------------------------------------------- > > > ------------------------------ > > Message: 8 > Date: Wed, 22 Feb 2012 05:08:51 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu, Jenny Vega > ? ? ? ? > Message-ID: > ? ? ? ?<1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of latex in > the back of the slides. I really don't feel comfortable mounting without > gloves because I heard that xyelene can cause cancer. Some people I know > personally has told me that this is not possible, but I read in some places > that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and > I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 9 > Date: Wed, 22 Feb 2012 13:15:28 +0000 > From: Debbie Faichney > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: Rene J Buesa , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ?, Jenny Vega > ? ? ? ? > Message-ID: > ? ? ? ?<8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> > Content-Type: text/plain; charset="iso-8859-1" > > If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. ?I use them and change every 30 minutes to avoid breakthrough. > > Debbie Faichney > Institute of Aquaculture > University of Stirling > UK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: 22 February 2012 13:09 > To: histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: Re: [Histonet] Does xylene cause skin cancer? > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of latex in > the back of the slides. I really don't feel comfortable mounting without > gloves because I heard that xyelene can cause cancer. Some people I know > personally has told me that this is not possible, but I read in some places > that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and > I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > The Sunday Times Scottish University of the Year 2009/2010 > The University of Stirling is a charity registered in Scotland, > ?number SC 011159. > > > > > ------------------------------ > > Message: 10 > Date: Wed, 22 Feb 2012 05:16:27 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Storage of Frozen Tissues > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ?, ? ?Dennis Hahn > ? ? ? ? > Message-ID: > ? ? ? ?<1329916587.26606.YahooMailClassic@web162104.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues in my tumor bank (-80?C) were kept in small (1inchx2inches) labeled zip-lock bags. I do not understand about your concern regarding "tissues being exposed to staff". > Inside the bags I used, the tissues were not exposed. If they were taken out for any study or procedures, universal precautions were taken. > Ren? J. > > --- On Wed, 2/22/12, Dennis Hahn wrote: > > > From: Dennis Hahn > Subject: [Histonet] Storage of Frozen Tissues > To: "'histonet@lists.utsouthwestern.edu'" > Date: Wednesday, February 22, 2012, 7:20 AM > > > How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? > > Thanks, > Dennis > > Dennis Hahn, HT (ASCP) > Histology Lab Supervisor > Cook Children's Medical Center > 801 7th Avenue > Ft. Worth, TX 76104 > (682) 885-6168 > > > > ________________________________ > > ---------------------------------------------------------------------------------------- > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. > > If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > --------------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 11 > Date: Wed, 22 Feb 2012 05:21:47 -0800 (PST) > From: Kim Merriam > Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: "Turner, Leandra" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1329916907.78300.YahooMailNeo@web130104.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material.? Here is my procedure: > > > 1.???? you need about 5X10^ cells per pellet > 2.???? spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube > 3.???? aspirate and resuspend in 15ml NBF and fix overnight @ RT > 4.???? spin @ 2000 rpm for 5 minutes and aspirate the NBF > 5.???? microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving) > 6.???? histogel should now be at liquid for and about 60C > 7.???? resuspend cell pellet in about 350ul of histogel > 8.???? place cell pellet in freezer or on ice for about 20 minutes to solidify > 9.???? using spatula, remove histogel pellet and place into cassette > 10.????????????????? post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed > 11.????????????????? process as usual > > > > Good luck! > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: "Turner, Leandra" > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, February 21, 2012 12:26 PM > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > > > > > > Hello Everyone, > > ? ? I am trying to find out a few of things about making cell pellets on > cerebrospinal fluids.? I would like to know: > > > > ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel > and has any tips or procedures they could share? > > > > ? ? 2. How to process the CSF pellets made with Histogel, do we need a > routine or stat process?? (we use a Sakura Tissue Tek) > > > > ? ? 3. Can you do IHC staining on the pellets? > > > > > > Thank you for any and all help in advance. > > > > Leandra Turner, HT (ASPC)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 12 > Date: Wed, 22 Feb 2012 05:23:15 -0800 (PST) > From: Kim Merriam > Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: "Turner, Leandra" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1329916995.72580.YahooMailNeo@web130102.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I forgot to mention, we prepare these specifically for IHC; so yes, you can do IHC on them. > Kim > > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: Kim Merriam > To: "Turner, Leandra" ; "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, February 22, 2012 8:21 AM > Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel > > > I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material.? Here is my procedure: > > > 1.???? you need about 5X10^ cells per pellet > 2.???? spin cells @ 2000 rpm for 5 minutes in a 50 ml conical tube > 3.???? aspirate and resuspend in 15ml NBF and fix overnight @ RT > 4.???? spin @ 2000 rpm for 5 minutes and aspirate the NBF > 5.???? microwave the histogel (thermo #HG-4000-012) for 15-20 seconds (loosen cap before microwaving) > 6.???? histogel should now be at liquid for and about 60C > 7.???? resuspend cell pellet in about 350ul of histogel > 8.???? place cell pellet in freezer or on ice for about 20 minutes to solidify > 9.???? using spatula, remove histogel pellet and place into cassette > 10.????????????????? post-fix in NBF for 2 hours (this step is very important); histogel will dissolve in processor if not fixed > 11.????????????????? process as usual > > > > Good luck! > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: "Turner, Leandra" > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, February 21, 2012 12:26 PM > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > > > > > > Hello Everyone, > > ? ? I am trying to find out a few of things about making cell pellets on > cerebrospinal fluids.? I would like to know: > > > > ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel > and has any tips or procedures they could share? > > > > ? ? 2. How to process the CSF pellets made with Histogel, do we need a > routine or stat process?? (we use a Sakura Tissue Tek) > > > > ? ? 3. Can you do IHC staining on the pellets? > > > > > > Thank you for any and all help in advance. > > > > Leandra Turner, HT (ASPC)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 13 > Date: Wed, 22 Feb 2012 14:51:31 +0000 > From: Hilary Smith > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: Debbie Faichney , Rene J Buesa > ? ? ? ?, "histonet@lists.utsouthwestern.edu" > ? ? ? ?, Jenny Vega > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset="iso-8859-1" > > You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: > > http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx > > According to our xylene MSDS: "The substance may be toxic to blood, kidneys, liver, > mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can > produce target organs damage." > > I would definitely use gloves if I were you. > > Hilary > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney > Sent: Wednesday, February 22, 2012 8:15 AM > To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: RE: [Histonet] Does xylene cause skin cancer? > > If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. ?I use them and change every 30 minutes to avoid breakthrough. > > Debbie Faichney > Institute of Aquaculture > University of Stirling > UK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: 22 February 2012 13:09 > To: histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: Re: [Histonet] Does xylene cause skin cancer? > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, ?number SC 011159. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Wed, 22 Feb 2012 10:00:59 -0500 > From: Melissa Phelan > Subject: [Histonet] Histotech & Histology Supervisor Job in Naples, FL > To: > Message-ID: > Content-Type: text/plain; ? ? ? charset="ISO-8859-1" > > Allied Search Partners has been retained for the following searches in > Florida. Please forward this along to anyone who you know that would be > interested in any of the following positions. We do offer a referral bonus. > > Please email a copy of updated resume to melissa@alliedsearchpartners.com > for a full job description. > > We have the following positions available: > > > Histotech > LOCATION: Naples, FL > DEPARTMENT & SCHEDULE: > Monday-Friday Day Shift/Full Time (6:30am-2:30pm) > > Histology Supervisor > LOCATION: Naples, FL > DEPARTMENT & SCHEDULE: > *Bachelor?s Degree Required, FL Histology Supervisor License Eligible > Monday-Friday Day Shift/Full Time > > -- > Melissa Phelan, President Laboratory Staffing > Allied Search Partners > http://www.linkedin.com/in/melissaphelan > ? P: 888-388-7571 > F: 888-388-7572 > C: 407-697-1175 > www.alliedsearchpartners.com > > > > > > > ------------------------------ > > Message: 15 > Date: Wed, 22 Feb 2012 15:26:43 +0000 > From: Amber McKenzie > Subject: [Histonet] Temp verifier slides - Ventana equipment > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? ?Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. > > > > > ------------------------------ > > Message: 16 > Date: Wed, 22 Feb 2012 17:14:40 +0000 > From: "Goins, Tresa" > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: Jenny Vega , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > Nitrile gloves are recommended for all the chemicals we use in our lab except acetone - for acetone latex is recommended. > We also coverslip by hand but we wear nitrile gloves without exception. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega > Sent: Tuesday, February 21, 2012 8:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Does xylene cause skin cancer? > > I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 28 > **************************************** From Jonathan.Cremer <@t> med.kuleuven.be Thu Feb 23 02:58:05 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Thu Feb 23 02:58:34 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A1C5FE@xmdb02.nch.kids> References: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> , <6D6BD1DE8A5571489398B392A38A715760A1C5FE@xmdb02.nch.kids> Message-ID: But the gloves in that Ansell list are reusable, whereas I suppose that you mean the disposable Kimberley-Clark gloves? The thickness would be very different in that case. And I wouldn't be able to mount a slide with thick, re-use gloves. :) In any case, the doctor for my professional yearly medical exam always tells me to only use nitrile gloves in combination with xylene, and that xylene will get through after a few minutes. So I just avoid getting xylene on my hands altogether by using forceps to handle wet slides while coverslipping. --- Jonathan Cremer Laboratory Technician Gastro-enterologie KUL CDG Bus 811; Labo Experimentele Immunologie; Herestraat 49; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Tony Henwood (SCHN) [tony.henwood@health.nsw.gov.au] Verzonden: donderdag 23 februari 2012 1:56 Aan: 'Hilary Smith'; Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Onderwerp: RE: [Histonet] Does xylene cause skin cancer? Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, whereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilary Smith Sent: Thursday, 23 February 2012 1:52 AM To: Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: "The substance may be toxic to blood, kidneys, liver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage." I would definitely use gloves if I were you. Hilary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney Sent: Wednesday, February 22, 2012 8:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. Ren? J. --- On Tue, 2/21/12, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Thu Feb 23 05:44:25 2012 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Thu Feb 23 05:44:46 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 In-Reply-To: References: <4f452d63.89c8ec0a.36be.6db9SMTPIN_ADDED@mx.google.com> Message-ID: <000001ccf220$7e8ff2a0$7bafd7e0$@com> ? You use regular superfrost plus slides for temperature verification of the thermal pads? The slides to use are the superfrost plus slides from Ventana that have a qc'd strip on them that will turn black if the temperatures of pads are at acceptable levels. How do you prove that with a plain superfrost plus slide? We use the suggested qc slides, but only do the test twice/yr instead of quarterly. We re-wrote our procedure to indicate that this is what we do. We then copy the slides onto paper and file for documentation purposes. You could also keep the slides, if you have the room to file and store them. CAP requires the written procedure as well as documentation that it has been done. Sheila, HT (ASCP) KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Wednesday, February 22, 2012 9:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 Message: 15 Date: Wed, 22 Feb 2012 15:26:43 +0000 From: Amber McKenzie Subject: [Histonet] Temp verifier slides - Ventana equipment To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. Amber, We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just fine. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Wed, Feb 22, 2012 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) > ? 2. RE: Processor Question (Davide Costanzo) > ? 3. Job Opening in Orange county California (Paula Lucas) > ? 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) > ? 5. Does xylene cause skin cancer? (Jenny Vega) > ? 6. RE: Does xylene cause skin cancer? (Settembre, Dana) > ? 7. Storage of Frozen Tissues (Dennis Hahn) > ? 8. Re: Does xylene cause skin cancer? (Rene J Buesa) > ? 9. RE: Does xylene cause skin cancer? (Debbie Faichney) > ?10. Re: Storage of Frozen Tissues (Rene J Buesa) > ?11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) > ?12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) > ?13. RE: Does xylene cause skin cancer? (Hilary Smith) > ?14. Histotech & Histology Supervisor Job in Naples, FL > ? ? ?(Melissa Phelan) > ?15. Temp verifier slides - Ventana equipment (Amber McKenzie) > ?16. RE: Does xylene cause skin cancer? (Goins, Tresa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: 21 Feb 2012 13:08:28 -0500 > From: "Darcy Bloch" > Subject: [Histonet] Seeking a Histology Supervisor in Connecticut > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > > ? Slone ?Partners ?seeks ?a ?Histology Supervisor for our hospital > based > ? laboratory > > > The ?successful ?candidate ? redesign ?and ?be ?great ?at ?managing change. ? people in the department, including 2 supervisors. ? experience is a plus. > > > > Qualified ? certification, ?with ? high-volume laboratory. > > > > Special ?features ?of this position: ? have the opportunity to help > redesign this > > > If ?you ?meet these qualifications ? for ?this ?position, ?please ?submit ?your ?resume ? darcyb@slonepartners.com. > > > > If ?you ? wish ?to be considered ? Tara Kochis at tara@slonepartners.com. > > > > All inquiries are kept confidential. > > > ------------------------------ > > Message: 2 > Date: Tue, 21 Feb 2012 10:33:27 -0800 > From: Davide Costanzo > Subject: RE: [Histonet] Processor Question > To: "Gauch, Vicki" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <-2296715788321707800@unknownmsgid> > Content-Type: text/plain; charset=ISO-8859-1 > > Design flaw in the screen display. It is in the way of the chamber > when opening chamber. If your not careful you will break the screen. > Happens fairly often. > > Sent from my Windows Phone > From: Gauch, Vicki > Sent: 2/21/2012 9:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processor Question Hi everyone, We are in the > market for new processors...and I was wondering if anyone could give > me some pros and cons for the Tissue Tek VIP 6 tissue processor - how > reliable are they? Ease of use ? Any known problems? ?Tissues process > well? ? You know....all the usual questions we all ask for new > equipment..... > > Thanks in advance for your help, > > Vicki Gauch > AMCH > Albany, NY > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 21 Feb 2012 12:23:07 -0800 > From: "Paula Lucas" > Subject: [Histonet] Job Opening in Orange county California > To: > Message-ID: > Content-Type: text/plain; ? ? ? charset="US-ASCII" > > A part-time histotech position is open in our histology laboratory. > > Tuesday through Saturday starting at 5 am. > > If anyone is interested, please either fax or email me your resume. > > Thank you, > > Paula Lucas > > Bio-Path Medical Group > > Fountain Valley, CA 92708 > > Fax: 714-755-2984 > > > > ------------------------------ > > Message: 4 > Date: Tue, 21 Feb 2012 17:22:57 -0500 > From: Amos Brooks > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > ? ? I haven't done a cellblock on CSF with Histogel, but I have has > success with some fairly scanty cell culture specimens. A short > processing cycle would be best. Try to make sure you have removed as > much supernatant as possible to keep the gel from shriveling during > processing. The IHC will be fine as long as the cells are suitably fixed ahead of time. > > Here is a link to the packaging insert for Histogel which describes > preparation of cell blocks. > https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf > > And here's a video of people using it for cytology specimens. > http://tinyurl.com/7jb522e > > Amos > > > On Tue, Feb 21, 2012 at 1:01 PM, > wrote: > >> Message: 15 >> Date: Tue, 21 Feb 2012 11:26:10 -0600 >> From: "Turner, Leandra" >> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel >> To: >> Message-ID: >> ? ? ? ?<3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> >> >> >> >> Hello Everyone, >> >> ? ?I am trying to find out a few of things about making cell pellets >> on cerebrospinal fluids. ?I would like to know: >> >> >> >> ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel >> and has any tips or procedures they could share? >> >> >> >> ? ? 2. How to process the CSF pellets made with Histogel, do we need >> a routine or stat process? ?(we use a Sakura Tissue Tek) >> >> >> >> ? ? 3. Can you do IHC staining on the pellets? >> >> >> >> >> >> Thank you for any and all help in advance. >> >> >> >> Leandra Turner, HT (ASPC)CM >> > > > ------------------------------ > > Message: 5 > Date: Tue, 21 Feb 2012 23:17:58 -0400 > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset=ISO-8859-1 > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of > latex in the back of the slides. I really don't feel comfortable > mounting without gloves because I heard that xyelene can cause cancer. > Some people I know personally has told me that this is not possible, > but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of > times and I am worried. > > > > Thanks. > > > ------------------------------ > > Message: 6 > Date: Wed, 22 Feb 2012 06:47:20 -0500 > From: "Settembre, Dana" > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: 'Jenny Vega' , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset="us-ascii" > > I would be worried too. > Wearing gloves in the lab is always a good practice and I believe, a > requirement in my lab whenever handling reagents or the possibility of > coming into contact with reagents. > You have the right to wear gloves if you want. > > Dana Settembre > University Hospital - UMDNJ > Newark, NJ > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny > Vega > Sent: Tuesday, February 21, 2012 10:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Does xylene cause skin cancer? > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of > latex in the back of the slides. I really don't feel comfortable > mounting without gloves because I heard that xyelene can cause cancer. > Some people I know personally has told me that this is not possible, > but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of > times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Wed, 22 Feb 2012 12:20:02 +0000 > From: Dennis Hahn > Subject: [Histonet] Storage of Frozen Tissues > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset="us-ascii" > > How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? > > Thanks, > Dennis > > Dennis Hahn, HT (ASCP) > Histology Lab Supervisor > Cook Children's Medical Center > 801 7th Avenue > Ft. Worth, TX 76104 > (682) 885-6168 > > > > ________________________________ > > ---------------------------------------------------------------------- > ------------------ > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. > > If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > ---------------------------------------------------------------------- > ----------------- > > > ------------------------------ > > Message: 8 > Date: Wed, 22 Feb 2012 05:08:51 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu, Jenny Vega > ? ? ? ? > Message-ID: > ? ? ? ? > <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of > latex in the back of the slides. I really don't feel comfortable > mounting without gloves because I heard that xyelene can cause cancer. > Some people I know personally has told me that this is not possible, > but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of > times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 9 > Date: Wed, 22 Feb 2012 13:15:28 +0000 > From: Debbie Faichney > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: Rene J Buesa , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ?, Jenny Vega > ? ? ? ? > Message-ID: > ? ? ? ? > <8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk> > Content-Type: text/plain; charset="iso-8859-1" > > If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. ?I use them and change every 30 minutes to avoid breakthrough. > > Debbie Faichney > Institute of Aquaculture > University of Stirling > UK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: 22 February 2012 13:09 > To: histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: Re: [Histonet] Does xylene cause skin cancer? > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my > coworkers don't like to use gloves because it leaves a residue of > latex in the back of the slides. I really don't feel comfortable > mounting without gloves because I heard that xyelene can cause cancer. > Some people I know personally has told me that this is not possible, > but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of > times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > The Sunday Times Scottish University of the Year 2009/2010 The > University of Stirling is a charity registered in Scotland, > ?number SC 011159. > > > > > ------------------------------ > > Message: 10 > Date: Wed, 22 Feb 2012 05:16:27 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Storage of Frozen Tissues > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ?, ? ?Dennis Hahn > ? ? ? ? > Message-ID: > ? ? ? ? > <1329916587.26606.YahooMailClassic@web162104.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues in my tumor bank (-80?C) were kept in small (1inchx2inches) labeled zip-lock bags. I do not understand about your concern regarding "tissues being exposed to staff". > Inside the bags I used, the tissues were not exposed. If they were taken out for any study or procedures, universal precautions were taken. > Ren? J. > > --- On Wed, 2/22/12, Dennis Hahn wrote: > > > From: Dennis Hahn > Subject: [Histonet] Storage of Frozen Tissues > To: "'histonet@lists.utsouthwestern.edu'" > > Date: Wednesday, February 22, 2012, 7:20 AM > > > How does everyone store tissues that are at -20 or -80? Currently we wrap the tissue well in foil and place in a labeled cassette. If shipped out, we double bag as required. Recently, a concern has been raised about the cassettes being a safety issue due to the fact that the tissue could be exposed to staff. Any ideas? > > Thanks, > Dennis > > Dennis Hahn, HT (ASCP) > Histology Lab Supervisor > Cook Children's Medical Center > 801 7th Avenue > Ft. Worth, TX 76104 > (682) 885-6168 > > > > ________________________________ > > ---------------------------------------------------------------------- > ------------------ > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. > > If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > ---------------------------------------------------------------------- > ----------------- _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 11 > Date: Wed, 22 Feb 2012 05:21:47 -0800 (PST) > From: Kim Merriam > Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: "Turner, Leandra" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1329916907.78300.YahooMailNeo@web130104.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material.? Here is my procedure: > > > 1.???? you need about 5X10^ cells per pellet 2.???? spin cells @ 2000 > rpm for 5 minutes in a 50 ml conical tube 3.???? aspirate and > resuspend in 15ml NBF and fix overnight @ RT 4.???? spin @ 2000 rpm > for 5 minutes and aspirate the NBF 5.???? microwave the histogel > (thermo #HG-4000-012) for 15-20 seconds (loosen cap before > microwaving) 6.???? histogel should now be at liquid for and about 60C > 7.???? resuspend cell pellet in about 350ul of histogel 8.???? place > cell pellet in freezer or on ice for about 20 minutes to solidify 9.???? > using spatula, remove histogel pellet and place into cassette 10.????????????????? > post-fix in NBF for 2 hours (this step is very important); histogel > will dissolve in processor if not fixed 11.????????????????? process > as usual > > > > Good luck! > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: "Turner, Leandra" > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, February 21, 2012 12:26 PM > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > > > > > > Hello Everyone, > > ? ? I am trying to find out a few of things about making cell pellets > on cerebrospinal fluids.? I would like to know: > > > > ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel > and has any tips or procedures they could share? > > > > ? ? 2. How to process the CSF pellets made with Histogel, do we need a > routine or stat process?? (we use a Sakura Tissue Tek) > > > > ? ? 3. Can you do IHC staining on the pellets? > > > > > > Thank you for any and all help in advance. > > > > Leandra Turner, HT (ASPC)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 12 > Date: Wed, 22 Feb 2012 05:23:15 -0800 (PST) > From: Kim Merriam > Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: "Turner, Leandra" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1329916995.72580.YahooMailNeo@web130102.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I forgot to mention, we prepare these specifically for IHC; so yes, you can do IHC on them. > Kim > > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: Kim Merriam > To: "Turner, Leandra" ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, February 22, 2012 8:21 AM > Subject: Re: [Histonet] Cytology CSF Cell Pellets made from Histogel > > > I work in a research lab, but I use histogel all the time to make FFPE blocks of cell culture material.? Here is my procedure: > > > 1.???? you need about 5X10^ cells per pellet 2.???? spin cells @ 2000 > rpm for 5 minutes in a 50 ml conical tube 3.???? aspirate and > resuspend in 15ml NBF and fix overnight @ RT 4.???? spin @ 2000 rpm > for 5 minutes and aspirate the NBF 5.???? microwave the histogel > (thermo #HG-4000-012) for 15-20 seconds (loosen cap before > microwaving) 6.???? histogel should now be at liquid for and about 60C > 7.???? resuspend cell pellet in about 350ul of histogel 8.???? place > cell pellet in freezer or on ice for about 20 minutes to solidify 9.???? > using spatula, remove histogel pellet and place into cassette 10.????????????????? > post-fix in NBF for 2 hours (this step is very important); histogel > will dissolve in processor if not fixed 11.????????????????? process > as usual > > > > Good luck! > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: "Turner, Leandra" > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, February 21, 2012 12:26 PM > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > > > > > > Hello Everyone, > > ? ? I am trying to find out a few of things about making cell pellets > on cerebrospinal fluids.? I would like to know: > > > > ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel > and has any tips or procedures they could share? > > > > ? ? 2. How to process the CSF pellets made with Histogel, do we need a > routine or stat process?? (we use a Sakura Tissue Tek) > > > > ? ? 3. Can you do IHC staining on the pellets? > > > > > > Thank you for any and all help in advance. > > > > Leandra Turner, HT (ASPC)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 13 > Date: Wed, 22 Feb 2012 14:51:31 +0000 > From: Hilary Smith > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: Debbie Faichney , Rene J Buesa > ? ? ? ?, "histonet@lists.utsouthwestern.edu" > ? ? ? ?, Jenny Vega > ? ? ? ? > Message-ID: > ? ? ? ? > > > > Content-Type: text/plain; charset="iso-8859-1" > > You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: > > http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K236 > 5_09_01_SN%20Chem%20Guide_v10.ashx > > According to our xylene MSDS: "The substance may be toxic to blood, > kidneys, liver, mucous membranes, bone marrow, central nervous system > (CNS). Repeated or prolonged exposure to the substance can produce target organs damage." > > I would definitely use gloves if I were you. > > Hilary > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie > Faichney > Sent: Wednesday, February 22, 2012 8:15 AM > To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: RE: [Histonet] Does xylene cause skin cancer? > > If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. ?I use them and change every 30 minutes to avoid breakthrough. > > Debbie Faichney > Institute of Aquaculture > University of Stirling > UK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: 22 February 2012 13:09 > To: histonet@lists.utsouthwestern.edu; Jenny Vega > Subject: Re: [Histonet] Does xylene cause skin cancer? > > There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. > Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. > Ren? J. > > --- On Tue, 2/21/12, Jenny Vega wrote: > > > From: Jenny Vega > Subject: [Histonet] Does xylene cause skin cancer? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 21, 2012, 10:17 PM > > > I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, ?number SC 011159. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Wed, 22 Feb 2012 10:00:59 -0500 > From: Melissa Phelan > Subject: [Histonet] Histotech & Histology Supervisor Job in Naples, FL > To: > Message-ID: > Content-Type: text/plain; ? ? ? charset="ISO-8859-1" > > Allied Search Partners has been retained for the following searches in > Florida. Please forward this along to anyone who you know that would > be interested in any of the following positions. We do offer a referral bonus. > > Please email a copy of updated resume to > melissa@alliedsearchpartners.com for a full job description. > > We have the following positions available: > > > Histotech > LOCATION: Naples, FL > DEPARTMENT & SCHEDULE: > Monday-Friday Day Shift/Full Time (6:30am-2:30pm) > > Histology Supervisor > LOCATION: Naples, FL > DEPARTMENT & SCHEDULE: > *Bachelor?s Degree Required, FL Histology Supervisor License Eligible > Monday-Friday Day Shift/Full Time > > -- > Melissa Phelan, President Laboratory Staffing Allied Search Partners > http://www.linkedin.com/in/melissaphelan > ? P: 888-388-7571 > F: 888-388-7572 > C: 407-697-1175 > www.alliedsearchpartners.com > > > > > > > ------------------------------ > > Message: 15 > Date: Wed, 22 Feb 2012 15:26:43 +0000 > From: Amber McKenzie > Subject: [Histonet] Temp verifier slides - Ventana equipment > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? ?Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. > > > > > ------------------------------ > > Message: 16 > Date: Wed, 22 Feb 2012 17:14:40 +0000 > From: "Goins, Tresa" > Subject: RE: [Histonet] Does xylene cause skin cancer? > To: Jenny Vega , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset="us-ascii" > > Nitrile gloves are recommended for all the chemicals we use in our lab except acetone - for acetone latex is recommended. > We also coverslip by hand but we wear nitrile gloves without exception. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny > Vega > Sent: Tuesday, February 21, 2012 8:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Does xylene cause skin cancer? > > I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. > > I have already gotten contact with xylene in my hands a couple of times and I am worried. > > > > Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 28 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachel.walls <@t> advocatehealth.com Thu Feb 23 05:55:42 2012 From: rachel.walls <@t> advocatehealth.com (Walls, Rachel) Date: Thu Feb 23 05:55:52 2012 Subject: [Histonet] ADIPOPHILIN Message-ID: Has anyone worked up the antibody, Adipophilin on the Ventana XT's? Thanks, Rachel rachel.walls@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From Marilyn.A.Weiss <@t> kp.org Thu Feb 23 06:00:38 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Feb 23 06:00:51 2012 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 02/23/2012 and will not return until 02/24/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From TNMayer <@t> mdanderson.org Thu Feb 23 07:56:33 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Feb 23 07:56:41 2012 Subject: [Histonet] RE: Does xylene cause skin cancer? Message-ID: We all have heard the reports that xylene causes cancer. It is a carcinogen. However only in cases where the user is extremely sensitive to xylene should you worry about a little bit getting on your skin every now and then. Don't bathe in it. Do not make it a habit. Wearing gloves (nitrile) and using other appropriate PPE should keep you safe. I wear gloves when I coverslip, change the machines and recycle. User safety is first, so check with the hospital to see what the safety department says. Change your gloves frequently when coverslipping. I have been a tech for 20 years and I am ok. I take the usual precautions with PPE and teach the same to my students. Toysha Mayer ------------------------------ Message: 2 Date: Wed, 22 Feb 2012 13:48:11 -0500 From: Bob Richmond Subject: [Histonet] Re: Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I don't know of any evidence that xylene causes skin cancer. Concern is with absorption through the skin. The most likely problem is with the bone marrow - leukemia and related diseases - from aromatic hydrocarbons (xylene, toluene, benzene) - which of course are present in resinous mounting media even in "xylene free" laboratories. Latex gloves dissolve rapidly. Nitrile rubber is more resistant, though not very. I don't know about vinyl examination gloves. I don't wear gloves in this situation, but obviously a pathologist gets much less exposure than a histotechnologist does. I certainly wouldn't argue with anyone who wanted to wear them. Bob Richmond Samurai Pathologist Knoxville TN ************************* From salim.inan <@t> gmail.com Thu Feb 23 08:08:01 2012 From: salim.inan <@t> gmail.com (Salim Yalcin Inan) Date: Thu Feb 23 08:08:19 2012 Subject: [Histonet] Histology Lab Donations In-Reply-To: <4f45ffe6.8125ec0a.5e36.ffffb67dSMTPIN_ADDED@mx.google.com> References: <4f45ffe6.8125ec0a.5e36.ffffb67dSMTPIN_ADDED@mx.google.com> Message-ID: <4F464841.8070702@gmail.com> Dear Histonet Members, I have been trying to set up a histology lab in our pharmacology department in Konya, Turkey. So far, I could only buy some basic stuff for cresyl violet staining in rat brain tissues. If you would like to get rid of some of your lab equipments and materials, or if you would like to donate some of them, I would like to accept any of your donations. Please contact me at: salim.inan@gmail.com Thank you very much in advance. Sincerely Yours, Salim -- Dr. Salim Yalcin Inan Assistant Professor Department of Pharmacology Meram Faculty of Medicine University of Konya 42080 Akyokus, Meram Konya, Turkey E-mail: salim.inan@gmail.com Phone: +90 530 967 9787 (Cellular) Phone: +90 332 223 6624 (Office-direct line) From NKonop <@t> chw.org Thu Feb 23 10:18:41 2012 From: NKonop <@t> chw.org (Konop, Nicole) Date: Thu Feb 23 10:18:46 2012 Subject: [Histonet] Cleaning Bond Bulk Reagent Containers Message-ID: Hello Everyone! I am interested to find out how people are cleaning their Bond Bulk Reagent Containers. I don't need a procedure. I know how to do that. My question is how do you clean these containers when you use them day and night and they don't have an opportunity to be cleaned and completely dry? I'm looking for any feedback on how you do this. Purchasing a second set of bulk reagent bottles is not an option. Thanks in advance for your feedback. Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)266-2524 Histology Department From rsrichmond <@t> gmail.com Thu Feb 23 12:33:00 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Feb 23 12:33:04 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? Message-ID: What's badly needed is a registry of death certificates of histotechnologists. The thing I'd want to examine would be the prevalence of myeloid leukemia and related diseases - known to be elevated in workers exposed to benzene. If such a correlation were found, it would mandate eliminating xylene from histology and cytology labs, and increasing precautions for handling resinous mounting media. The American Medical Association maintains such a registry of American physicians (or used to). It was used to establish that exposure to ionizing radiation (fluoroscopes and other X-ray equipment) was correlated with deaths from myeloid leukemia. Pathologists didn't have any particular problems - here I'd wonder about formaldehyde exposure and upper airway cancer. Pathologists get more exposure to formaldehyde than do histotechnologists. Bob Richmond Samurai Pathologist Knoxville TN From rory.pritchard001 <@t> gmail.com Thu Feb 23 12:36:02 2012 From: rory.pritchard001 <@t> gmail.com (Rory Pritchard) Date: Thu Feb 23 12:36:17 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 30 In-Reply-To: <4f467ed7.8125ec0a.56e6.ffffe1d7SMTPIN_ADDED@mx.google.com> References: <4f467ed7.8125ec0a.56e6.ffffe1d7SMTPIN_ADDED@mx.google.com> Message-ID: <43F49FF3-0EB6-4F80-AEE1-1DFB31BD5FFE@gmail.com> Message 5: xylene Xylene is toxic and you should always wear gloves and a face mask when dealing with it. Dermal absorption is a lot slower than inhalation, but contact with organic solvents such as xylene is bad in general. With the issue of latex residue left on the slide, try using nitrile gloves. We use nitrile in our lab and we never have an issue with residue left on the slides. You may also want to wear two gloves on the hand that might come in contact with xylene because it can eat through gloves. I haven't found anything linking xylene as a human carcinogen, but it's toxic to the central nervous system as well as a known teratogen, so limiting any contact is advised. You can also use histoclear, another organic solvent that works really well that is far less toxic than xylene. On Feb 23, 2012, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Re: Histonet Digest, Vol 99, Issue 28 (Sheila Fonner) > 2. ADIPOPHILIN (Walls, Rachel) > 3. out of office (Marilyn.A.Weiss@kp.org) > 4. RE: Does xylene cause skin cancer? (Mayer,Toysha N) > 5. Histology Lab Donations (Salim Yalcin Inan) > 6. Cleaning Bond Bulk Reagent Containers (Konop, Nicole) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 23 Feb 2012 06:44:25 -0500 > From: "Sheila Fonner" > Subject: RE: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > To: "'Madeleine Huey'" , > > Message-ID: <000001ccf220$7e8ff2a0$7bafd7e0$@com> > Content-Type: text/plain; charset="iso-8859-1" > > ? You use regular superfrost plus slides for temperature verification of the > thermal pads? The slides to use are the superfrost plus slides from Ventana > that have a qc'd strip on them that will turn black if the temperatures of > pads are at acceptable levels. How do you prove that with a plain > superfrost plus slide? > > We use the suggested qc slides, but only do the test twice/yr instead of > quarterly. We re-wrote our procedure to indicate that this is what we do. > We then copy the slides onto paper and file for documentation purposes. You > could also keep the slides, if you have the room to file and store them. > CAP requires the written procedure as well as documentation that it has been > done. > > Sheila, HT (ASCP) > KDL Pathology > Knoxville, TN > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine > Huey > Sent: Wednesday, February 22, 2012 9:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > > Message: 15 > Date: Wed, 22 Feb 2012 15:26:43 +0000 > From: Amber McKenzie > Subject: [Histonet] Temp verifier slides - Ventana equipment > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > Do y'all run the temp verifier slides for the quarterly maintenance for the > Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 > months, esp if you have multiple pieces of equipment. > > Amber, > > We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just > fine. > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org > > On Wed, Feb 22, 2012 at 10:01 AM, > wrote: >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) >> ? 2. RE: Processor Question (Davide Costanzo) >> ? 3. Job Opening in Orange county California (Paula Lucas) >> ? 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) >> ? 5. Does xylene cause skin cancer? (Jenny Vega) >> ? 6. RE: Does xylene cause skin cancer? (Settembre, Dana) >> ? 7. Storage of Frozen Tissues (Dennis Hahn) >> ? 8. Re: Does xylene cause skin cancer? (Rene J Buesa) >> ? 9. RE: Does xylene cause skin cancer? (Debbie Faichney) >> ?10. Re: Storage of Frozen Tissues (Rene J Buesa) >> ?11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) >> ?12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) >> ?13. RE: Does xylene cause skin cancer? (Hilary Smith) >> ?14. Histotech & Histology Supervisor Job in Naples, FL >> ? ? ?(Melissa Phelan) >> ?15. Temp verifier slides - Ventana equipment (Amber McKenzie) >> ?16. RE: Does xylene cause skin cancer? (Goins, Tresa) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: 21 Feb 2012 13:08:28 -0500 >> From: "Darcy Bloch" >> Subject: [Histonet] Seeking a Histology Supervisor in Connecticut >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset="us-ascii" >> >> >> ? Slone ?Partners ?seeks ?a ?Histology Supervisor for our hospital >> based >> ? laboratory >> >> >> The ?successful ?candidate ? redesign ?and ?be ?great ?at ?managing > change. ? people in the department, including 2 supervisors. ? experience is > a plus. >> >> >> >> Qualified ? certification, ?with ? high-volume laboratory. >> >> >> >> Special ?features ?of this position: ? have the opportunity to help >> redesign this >> >> >> If ?you ?meet these qualifications ? for ?this ?position, ?please ?submit > ?your ?resume ? darcyb@slonepartners.com. >> >> >> >> If ?you ? wish ?to be considered ? Tara Kochis at tara@slonepartners.com. >> >> >> >> All inquiries are kept confidential. >> >> >> ------------------------------ >> >> Message: 2 >> Date: Tue, 21 Feb 2012 10:33:27 -0800 >> From: Davide Costanzo >> Subject: RE: [Histonet] Processor Question >> To: "Gauch, Vicki" , >> ? ? ? ?"histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: <-2296715788321707800@unknownmsgid> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Design flaw in the screen display. It is in the way of the chamber >> when opening chamber. If your not careful you will break the screen. >> Happens fairly often. >> >> Sent from my Windows Phone >> From: Gauch, Vicki >> Sent: 2/21/2012 9:18 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Processor Question Hi everyone, We are in the >> market for new processors...and I was wondering if anyone could give >> me some pros and cons for the Tissue Tek VIP 6 tissue processor - how >> reliable are they? Ease of use ? Any known problems? ?Tissues process >> well? ? You know....all the usual questions we all ask for new >> equipment..... >> >> Thanks in advance for your help, >> >> Vicki Gauch >> AMCH >> Albany, NY >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Tue, 21 Feb 2012 12:23:07 -0800 >> From: "Paula Lucas" >> Subject: [Histonet] Job Opening in Orange county California >> To: >> Message-ID: >> Content-Type: text/plain; ? ? ? charset="US-ASCII" >> >> A part-time histotech position is open in our histology laboratory. >> >> Tuesday through Saturday starting at 5 am. >> >> If anyone is interested, please either fax or email me your resume. >> >> Thank you, >> >> Paula Lucas >> >> Bio-Path Medical Group >> >> Fountain Valley, CA 92708 >> >> Fax: 714-755-2984 >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Tue, 21 Feb 2012 17:22:57 -0500 >> From: Amos Brooks >> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hi, >> ? ? I haven't done a cellblock on CSF with Histogel, but I have has >> success with some fairly scanty cell culture specimens. A short >> processing cycle would be best. Try to make sure you have removed as >> much supernatant as possible to keep the gel from shriveling during >> processing. The IHC will be fine as long as the cells are suitably fixed > ahead of time. >> >> Here is a link to the packaging insert for Histogel which describes >> preparation of cell blocks. >> https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf >> >> And here's a video of people using it for cytology specimens. >> http://tinyurl.com/7jb522e >> >> Amos >> >> >> On Tue, Feb 21, 2012 at 1:01 PM, >> wrote: >> >>> Message: 15 >>> Date: Tue, 21 Feb 2012 11:26:10 -0600 >>> From: "Turner, Leandra" >>> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel >>> To: >>> Message-ID: >>> ? ? ? ?<3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> >>> Content-Type: text/plain; ? ? ? charset="us-ascii" >>> >>> >>> >>> >>> >>> Hello Everyone, >>> >>> ? ?I am trying to find out a few of things about making cell pellets >>> on cerebrospinal fluids. ?I would like to know: >>> >>> >>> >>> ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel >>> and has any tips or procedures they could share? >>> >>> >>> >>> ? ? 2. How to process the CSF pellets made with Histogel, do we need >>> a routine or stat process? ?(we use a Sakura Tissue Tek) >>> >>> >>> >>> ? ? 3. Can you do IHC staining on the pellets? >>> >>> >>> >>> >>> >>> Thank you for any and all help in advance. >>> >>> >>> >>> Leandra Turner, HT (ASPC)CM >>> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Tue, 21 Feb 2012 23:17:58 -0400 >> From: Jenny Vega >> Subject: [Histonet] Does xylene cause skin cancer? >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> I am asking this because in my job we mount slides by hand, and my >> coworkers don't like to use gloves because it leaves a residue of >> latex in the back of the slides. I really don't feel comfortable >> mounting without gloves because I heard that xyelene can cause cancer. >> Some people I know personally has told me that this is not possible, >> but I read in some places that xylene could a possible carcinogen. >> >> I have already gotten contact with xylene in my hands a couple of >> times and I am worried. >> >> >> >> Thanks. >> >> >> ------------------------------ >> >> Message: 6 >> Date: Wed, 22 Feb 2012 06:47:20 -0500 >> From: "Settembre, Dana" >> Subject: RE: [Histonet] Does xylene cause skin cancer? >> To: 'Jenny Vega' , >> ? ? ? ?"histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset="us-ascii" >> >> I would be worried too. >> Wearing gloves in the lab is always a good practice and I believe, a >> requirement in my lab whenever handling reagents or the possibility of >> coming into contact with reagents. >> You have the right to wear gloves if you want. >> >> Dana Settembre >> University Hospital - UMDNJ >> Newark, NJ >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny >> Vega >> Sent: Tuesday, February 21, 2012 10:18 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Does xylene cause skin cancer? >> >> I am asking this because in my job we mount slides by hand, and my >> coworkers don't like to use gloves because it leaves a residue of >> latex in the back of the slides. I really don't feel comfortable >> mounting without gloves because I heard that xyelene can cause cancer. >> Some people I know personally has told me that this is not possible, >> but I read in some places that xylene could a possible carcinogen. >> >> I have already gotten contact with xylene in my hands a couple of >> times and I am worried. >> >> >> >> Thanks. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 7 >> Date: Wed, 22 Feb 2012 12:20:02 +0000 >> From: Dennis Hahn >> Subject: [Histonet] Storage of Frozen Tissues >> To: "'histonet@lists.utsouthwestern.edu'" >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset="us-ascii" >> >> How does everyone store tissues that are at -20 or -80? Currently we wrap > the tissue well in foil and place in a labeled cassette. If shipped out, we > double bag as required. Recently, a concern has been raised about the > cassettes being a safety issue due to the fact that the tissue could be > exposed to staff. Any ideas? >> >> Thanks, >> Dennis >> >> Dennis Hahn, HT (ASCP) >> Histology Lab Supervisor >> Cook Children's Medical Center >> 801 7th Avenue >> Ft. Worth, TX 76104 >> (682) 885-6168 >> >> >> >> ________________________________ >> >> ---------------------------------------------------------------------- >> ------------------ >> Cook Children's Health Care System >> >> This e-mail, facsimile, or letter and any files or attachments transmitted > may contain information that is confidential and privileged. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient, any disclosure, copying, printing, or use of > this information is strictly prohibited and possibly a violation of federal > or state law and regulations. >> >> If you have received this information in error, please notify Cook > Children's Health Care System immediately at (682)885-4000 or via e-mail at > compliance@cookchildrens.org. Cook Children's Health Care System, its > subsidiaries, and affiliates hereby claim all applicable privileges related > to this information. >> ---------------------------------------------------------------------- >> ----------------- >> >> >> ------------------------------ >> >> Message: 8 >> Date: Wed, 22 Feb 2012 05:08:51 -0800 (PST) >> From: Rene J Buesa >> Subject: Re: [Histonet] Does xylene cause skin cancer? >> To: histonet@lists.utsouthwestern.edu, Jenny Vega >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> There is no evidence in the literature about skin cancer produced by > xylene, although dermatitis are well documented. >> Regardless you should use gloves whenever your hands can get in contact > with any chemical as a good safety practice. If your colleagues do not want > to use gloves, that is their prerogative, as is yours to wear them. >> Ren? J. >> >> --- On Tue, 2/21/12, Jenny Vega wrote: >> >> >> From: Jenny Vega >> Subject: [Histonet] Does xylene cause skin cancer? >> To: histonet@lists.utsouthwestern.edu >> Date: Tuesday, February 21, 2012, 10:17 PM >> >> >> I am asking this because in my job we mount slides by hand, and my >> coworkers don't like to use gloves because it leaves a residue of >> latex in the back of the slides. I really don't feel comfortable >> mounting without gloves because I heard that xyelene can cause cancer. >> Some people I know personally has From JCBRITTON <@t> Cheshire-Med.COM Thu Feb 23 12:41:47 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Thu Feb 23 12:41:54 2012 Subject: [Histonet] Cleaning Bond Bulk Reagent Containers In-Reply-To: References: Message-ID: Try after cleaning them rinse them with 100% alcohol and using a hair dryer! You really should purchase a spare set anyway, because sometime there are issues with the nozzles and the bond will not run without them. Your production would stop immediately and you would still have to purchase the bottles anyhow! Josie Britton HT Cheshire Medical Center Keene NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Thursday, February 23, 2012 11:19 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning Bond Bulk Reagent Containers Hello Everyone! I am interested to find out how people are cleaning their Bond Bulk Reagent Containers. I don't need a procedure. I know how to do that. My question is how do you clean these containers when you use them day and night and they don't have an opportunity to be cleaned and completely dry? I'm looking for any feedback on how you do this. Purchasing a second set of bulk reagent bottles is not an option. Thanks in advance for your feedback. Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)266-2524 Histology Department _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rory.pritchard001 <@t> gmail.com Thu Feb 23 12:42:32 2012 From: rory.pritchard001 <@t> gmail.com (Rory Pritchard) Date: Thu Feb 23 12:42:46 2012 Subject: [Histonet] Re: does xylene cause skin cancer In-Reply-To: <4f467ed7.8125ec0a.56e6.ffffe1d7SMTPIN_ADDED@mx.google.com> References: <4f467ed7.8125ec0a.56e6.ffffe1d7SMTPIN_ADDED@mx.google.com> Message-ID: Xylene is toxic and you should always wear gloves and a face mask when dealing with it. Dermal absorption is a lot slower than inhalation, but contact with organic solvents such as xylene is bad in general. With the issue of latex residue left on the slide, try using nitrile gloves. We use nitrile in our lab and we never have an issue with residue left on the slides. You may also want to wear two gloves on the hand that might come in contact with xylene because it can eat through gloves. I haven't found anything linking xylene as a human carcinogen, but it's toxic to the central nervous system as well as a known teratogen, so limiting any contact is advised. You can also use histoclear, another organic solvent that works really well that is far less toxic than xylene. Best, Rory On Feb 23, 2012, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Re: Histonet Digest, Vol 99, Issue 28 (Sheila Fonner) > 2. ADIPOPHILIN (Walls, Rachel) > 3. out of office (Marilyn.A.Weiss@kp.org) > 4. RE: Does xylene cause skin cancer? (Mayer,Toysha N) > 5. Histology Lab Donations (Salim Yalcin Inan) > 6. Cleaning Bond Bulk Reagent Containers (Konop, Nicole) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 23 Feb 2012 06:44:25 -0500 > From: "Sheila Fonner" > Subject: RE: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > To: "'Madeleine Huey'" , > > Message-ID: <000001ccf220$7e8ff2a0$7bafd7e0$@com> > Content-Type: text/plain; charset="iso-8859-1" > > ? You use regular superfrost plus slides for temperature verification of the > thermal pads? The slides to use are the superfrost plus slides from Ventana > that have a qc'd strip on them that will turn black if the temperatures of > pads are at acceptable levels. How do you prove that with a plain > superfrost plus slide? > > We use the suggested qc slides, but only do the test twice/yr instead of > quarterly. We re-wrote our procedure to indicate that this is what we do. > We then copy the slides onto paper and file for documentation purposes. You > could also keep the slides, if you have the room to file and store them. > CAP requires the written procedure as well as documentation that it has been > done. > > Sheila, HT (ASCP) > KDL Pathology > Knoxville, TN > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine > Huey > Sent: Wednesday, February 22, 2012 9:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > > Message: 15 > Date: Wed, 22 Feb 2012 15:26:43 +0000 > From: Amber McKenzie > Subject: [Histonet] Temp verifier slides - Ventana equipment > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E@JERRY.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > Do y'all run the temp verifier slides for the quarterly maintenance for the > Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 > months, esp if you have multiple pieces of equipment. > > Amber, > > We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just > fine. > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org > > On Wed, Feb 22, 2012 at 10:01 AM, > wrote: >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) >> ? 2. RE: Processor Question (Davide Costanzo) >> ? 3. Job Opening in Orange county California (Paula Lucas) >> ? 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) >> ? 5. Does xylene cause skin cancer? (Jenny Vega) >> ? 6. RE: Does xylene cause skin cancer? (Settembre, Dana) >> ? 7. Storage of Frozen Tissues (Dennis Hahn) >> ? 8. Re: Does xylene cause skin cancer? (Rene J Buesa) >> ? 9. RE: Does xylene cause skin cancer? (Debbie Faichney) >> ?10. Re: Storage of Frozen Tissues (Rene J Buesa) >> ?11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) >> ?12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) >> ?13. RE: Does xylene cause skin cancer? (Hilary Smith) >> ?14. Histotech & Histology Supervisor Job in Naples, FL >> ? ? ?(Melissa Phelan) >> ?15. Temp verifier slides - Ventana equipment (Amber McKenzie) >> ?16. RE: Does xylene cause skin cancer? (Goins, Tresa) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: 21 Feb 2012 13:08:28 -0500 >> From: "Darcy Bloch" >> Subject: [Histonet] Seeking a Histology Supervisor in Connecticut >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset="us-ascii" >> >> >> ? Slone ?Partners ?seeks ?a ?Histology Supervisor for our hospital >> based >> ? laboratory >> >> >> The ?successful ?candidate ? redesign ?and ?be ?great ?at ?managing > change. ? people in the department, including 2 supervisors. ? experience is > a plus. >> >> >> >> Qualified ? certification, ?with ? high-volume laboratory. >> >> >> >> Special ?features ?of this position: ? have the opportunity to help >> redesign this >> >> >> If ?you ?meet these qualifications ? for ?this ?position, ?please ?submit > ?your ?resume ? darcyb@slonepartners.com. >> >> >> >> If ?you ? wish ?to be considered ? Tara Kochis at tara@slonepartners.com. >> >> >> >> All inquiries are kept confidential. >> >> >> ------------------------------ >> >> Message: 2 >> Date: Tue, 21 Feb 2012 10:33:27 -0800 >> From: Davide Costanzo >> Subject: RE: [Histonet] Processor Question >> To: "Gauch, Vicki" , >> ? ? ? ?"histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: <-2296715788321707800@unknownmsgid> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Design flaw in the screen display. It is in the way of the chamber >> when opening chamber. If your not careful you will break the screen. >> Happens fairly often. >> >> Sent from my Windows Phone >> From: Gauch, Vicki >> Sent: 2/21/2012 9:18 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Processor Question Hi everyone, We are in the >> market for new processors...and I was wondering if anyone could give >> me some pros and cons for the Tissue Tek VIP 6 tissue processor - how >> reliable are they? Ease of use ? Any known problems? ?Tissues process >> well? ? You know....all the usual questions we all ask for new >> equipment..... >> >> Thanks in advance for your help, >> >> Vicki Gauch >> AMCH >> Albany, NY >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Tue, 21 Feb 2012 12:23:07 -0800 >> From: "Paula Lucas" >> Subject: [Histonet] Job Opening in Orange county California >> To: >> Message-ID: >> Content-Type: text/plain; ? ? ? charset="US-ASCII" >> >> A part-time histotech position is open in our histology laboratory. >> >> Tuesday through Saturday starting at 5 am. >> >> If anyone is interested, please either fax or email me your resume. >> >> Thank you, >> >> Paula Lucas >> >> Bio-Path Medical Group >> >> Fountain Valley, CA 92708 >> >> Fax: 714-755-2984 >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Tue, 21 Feb 2012 17:22:57 -0500 >> From: Amos Brooks >> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hi, >> ? ? I haven't done a cellblock on CSF with Histogel, but I have has >> success with some fairly scanty cell culture specimens. A short >> processing cycle would be best. Try to make sure you have removed as >> much supernatant as possible to keep the gel from shriveling during >> processing. The IHC will be fine as long as the cells are suitably fixed > ahead of time. >> >> Here is a link to the packaging insert for Histogel which describes >> preparation of cell blocks. >> https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf >> >> And here's a video of people using it for cytology specimens. >> http://tinyurl.com/7jb522e >> >> Amos >> >> >> On Tue, Feb 21, 2012 at 1:01 PM, >> wrote: >> >>> Message: 15 >>> Date: Tue, 21 Feb 2012 11:26:10 -0600 >>> From: "Turner, Leandra" >>> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel >>> To: >>> Message-ID: >>> ? ? ? ?<3D79F47DC92B204F9E5D35C885DFC5CB010AB869@AUSEX2VS1.seton.org> >>> Content-Type: text/plain; ? ? ? charset="us-ascii" >>> >>> >>> >>> >>> >>> Hello Everyone, >>> >>> ? ?I am trying to find out a few of things about making cell pellets >>> on cerebrospinal fluids. ?I would like to know: >>> >>> >>> >>> ? ? 1. If anyone has ever made cell pellets from CSF's using Histogel >>> and has any tips or procedures they could share? >>> >>> >>> >>> ? ? 2. How to process the CSF pellets made with Histogel, do we need >>> a routine or stat process? ?(we use a Sakura Tissue Tek) >>> >>> >>> >>> ? ? 3. Can you do IHC staining on the pellets? >>> >>> >>> >>> >>> >>> Thank you for any and all help in advance. >>> >>> >>> >>> Leandra Turner, HT (ASPC)CM >>> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Tue, 21 Feb 2012 23:17:58 -0400 >> From: Jenny Vega >> Subject: [Histonet] Does xylene cause skin cancer? >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> I am asking this because in my job we mount slides by hand, and my >> coworkers don't like to use gloves because it leaves a residue of >> latex in the back of the slides. I really don't feel comfortable >> mounting without gloves because I heard that xyelene can cause cancer. >> Some people I know personally has told me that this is not possible, >> but I read in some places that xylene could a possible carcinogen. >> >> I have already gotten contact with xylene in my hands a couple of >> times and I am worried. >> >> >> >> Thanks. >> >> >> ------------------------------ >> >> Message: 6 >> Date: Wed, 22 Feb 2012 06:47:20 -0500 >> From: "Settembre, Dana" >> Subject: RE: [Histonet] Does xylene cause skin cancer? >> To: 'Jenny Vega' , >> ? ? ? ?"histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset="us-ascii" >> >> I would be worried too. >> Wearing gloves in the lab is always a good practice and I believe, a >> requirement in my lab whenever handling reagents or the possibility of >> coming into contact with reagents. >> You have the right to wear gloves if you want. >> >> Dana Settembre >> University Hospital - UMDNJ >> Newark, NJ >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny >> Vega >> Sent: Tuesday, February 21, 2012 10:18 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Does xylene cause skin cancer? >> >> I am asking this because in my job we mount slides by hand, and my >> coworkers don't like to use gloves because it leaves a residue of >> latex in the back of the slides. I really don't feel comfortable >> mounting without gloves because I heard that xyelene can cause cancer. >> Some people I know personally has told me that this is not possible, >> but I read in some places that xylene could a possible carcinogen. >> >> I have already gotten contact with xylene in my hands a couple of >> times and I am worried. >> >> >> >> Thanks. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 7 >> Date: Wed, 22 Feb 2012 12:20:02 +0000 >> From: Dennis Hahn >> Subject: [Histonet] Storage of Frozen Tissues >> To: "'histonet@lists.utsouthwestern.edu'" >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> >> Content-Type: text/plain; charset="us-ascii" >> >> How does everyone store tissues that are at -20 or -80? Currently we wrap > the tissue well in foil and place in a labeled cassette. If shipped out, we > double bag as required. Recently, a concern has been raised about the > cassettes being a safety issue due to the fact that the tissue could be > exposed to staff. Any ideas? >> >> Thanks, >> Dennis >> >> Dennis Hahn, HT (ASCP) >> Histology Lab Supervisor >> Cook Children's Medical Center >> 801 7th Avenue >> Ft. Worth, TX 76104 >> (682) 885-6168 >> >> >> >> ________________________________ >> >> ---------------------------------------------------------------------- >> ------------------ >> Cook Children's Health Care System >> >> This e-mail, facsimile, or letter and any files or attachments transmitted > may contain information that is confidential and privileged. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient, any disclosure, copying, printing, or use of > this information is strictly prohibited and possibly a violation of federal > or state law and regulations. >> >> If you have received this information in error, please notify Cook > Children's Health Care System immediately at (682)885-4000 or via e-mail at > compliance@cookchildrens.org. Cook Children's Health Care System, its > subsidiaries, and affiliates hereby claim all applicable privileges related > to this information. >> ---------------------------------------------------------------------- >> ----------------- >> >> >> ------------------------------ >> >> Message: 8 >> Date: Wed, 22 Feb 2012 05:08:51 -0800 (PST) >> From: Rene J Buesa >> Subject: Re: [Histonet] Does xylene cause skin cancer? >> To: histonet@lists.utsouthwestern.edu, Jenny Vega >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> There is no evidence in the literature about skin cancer produced by > xylene, although dermatitis are well documented. >> Regardless you should use gloves whenever your hands can get in contact > with any chemical as a good safety practice. If your colleagues do not want > to use gloves, that is their prerogative, as is yours to wear them. >> Ren? J. >> >> --- On Tue, 2/21/12, Jenny Vega wrote: >> >> >> From: Jenny Vega >> Subject: [Histonet] Does xylene cause skin cancer? >> To: histonet@lists.utsouthwestern.edu >> Date: Tuesday, February 21, 2012, 10:17 PM >> >> >> I am asking this because in my job we mount slides by hand, and my >> coworkers don't like to use gloves because it leaves a residue of >> latex in the back of the slides. I really don't feel comfortable >> mounting without gloves because I heard that xyelene can cause cancer. >> Some people I know personally has From pkarlisch <@t> hmc.psu.edu Thu Feb 23 13:12:25 2012 From: pkarlisch <@t> hmc.psu.edu (Karlisch, Patricia) Date: Thu Feb 23 13:12:30 2012 Subject: [Histonet] Adenovirus controls for paraffin sections Message-ID: Hi, Can anyone recommend Adenovirus controls for paraffin sections. I can not find a vendor that sells these controls any longer. Any help would be appreciated. Pat Karlisch *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From rsrichmond <@t> gmail.com Thu Feb 23 13:24:18 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Feb 23 13:24:24 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? In-Reply-To: References: Message-ID: As I failed to mention in my earlier post - the problem with a registry is that many (most, in my personal experience) American histotechnologists are completely uncertified, and would not appear in any registry. One would hazard a guess that these uncertified techs get the most xylene exposure. Bob Richmond ***************************************** On Thu, Feb 23, 2012 at 1:33 PM, Bob Richmond wrote: > What's badly needed is a registry of death certificates of > histotechnologists. The thing I'd want to examine would be the > prevalence of myeloid leukemia and related diseases - known to be > elevated in workers exposed to benzene. If such a correlation were > found, it would mandate eliminating xylene from histology and cytology > labs, and increasing precautions for handling resinous mounting media. > > The American Medical Association maintains such a registry of American > physicians (or used to). It was used to establish that exposure to > ionizing radiation (fluoroscopes and other X-ray equipment) was > correlated with deaths from myeloid leukemia. Pathologists didn't have > any particular problems - here I'd wonder about formaldehyde exposure > and upper airway cancer. Pathologists get more exposure to > formaldehyde than do histotechnologists. > > Bob Richmond > Samurai Pathologist > Knoxville TN From b427297 <@t> aol.com Thu Feb 23 14:43:59 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Feb 23 14:44:16 2012 Subject: [Histonet] Does xylene cause skin cancer? In-Reply-To: <8CEC0501C208C7C-728-5334F@webmail-m021.sysops.aol.com> References: <1329916131.63705.YahooMailClassic@web162101.mail.bf1.yahoo.com><8ED3F2CA5B78E142B8193376C57330F8F9087CD04A@EXCH2007.ad.stir.ac.uk>, <6D6BD1DE8A5571489398B392A38A715760A1C5FE@xmdb02.nch.kids> <8CEC0501C208C7C-728-5334F@webmail-m021.sysops.aol.com> Message-ID: <8CEC083012C63D0-20D0-50FA@webmail-m129.sysops.aol.com> -----Original Message----- From: Jackie O'Connor To: Jonathan.Cremer Sent: Thu, Feb 23, 2012 8:39 am Subject: Re: [Histonet] Does xylene cause skin cancer? I grew up in histology before MSDS's and OSHA. We practically bathed in xylene, which is no excuse for ignoring safety precautions now. In all my years and places I've worked, I don't know anyone who has developed cancer because of xylene exposure per se - you can all say "that they know of". I can honestly say, out of the hundreds of people I met and worked with, personally I only know of a few histotechs and pathologists who actually died from cancer over the last 40 years. I do, however, know a larger number of histotechs who have suffered unusual miscarriages, still births, and had children with unusual birth defects. That is something that no one (like the NSH or histology chemical providers) has really ever looked into. Common sense should always be your first line of defense, people. Don't get it on your skin if you can avoid contact, but if you're that paranoid about the chemicals you work with, you are probably in the wrong line of work. If you look at some of the special stains we work with, they are scarier than xylene. I'm jus' sayin . . . Jackie O' -----Original Message----- From: Jonathan Cremer To: Tony Henwood (SCHN) ; 'Hilary Smith' ; Debbie Faichney ; Rene J Buesa ; histonet ; Jenny Vega Sent: Thu, Feb 23, 2012 2:59 am Subject: RE: [Histonet] Does xylene cause skin cancer? But the gloves in that Ansell list are reusable, whereas I suppose that you mean he disposable Kimberley-Clark gloves? The thickness would be very different in hat case. And I wouldn't be able to mount a slide with thick, re-use gloves. :) In any case, the doctor for my professional yearly medical exam always tells me o only use nitrile gloves in combination with xylene, and that xylene will get hrough after a few minutes. So I just avoid getting xylene on my hands ltogether by using forceps to handle wet slides while coverslipping. -- onathan Cremer aboratory Technician astro-enterologie KUL CDG Bus 811; Labo Experimentele Immunologie; Herestraat 49; 3000 Leuven; elgium _______________________________________ an: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] amens Tony Henwood (SCHN) [tony.henwood@health.nsw.gov.au] erzonden: donderdag 23 februari 2012 1:56 an: 'Hilary Smith'; Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; enny Vega nderwerp: RE: [Histonet] Does xylene cause skin cancer? Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, hereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards ony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) aboratory Manager & Senior Scientist el: 612 9845 3306 ax: 612 9845 3318 he children's hospital at westmead nr Hawkesbury Road and Hainsworth Street, Westmead ocked Bag 4001, Westmead NSW 2145, AUSTRALIA ----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Hilary Smith ent: Thursday, 23 February 2012 1:52 AM o: Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega ubject: RE: [Histonet] Does xylene cause skin cancer? You might want to go to something with even greater chemical resistance - thin itrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: "The substance may be toxic to blood, kidneys, iver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or rolonged exposure to the substance can produce target organs damage." I would definitely use gloves if I were you. Hilary ----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Debbie Faichney ent: Wednesday, February 22, 2012 8:15 AM o: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega ubject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher hemical resistance than latex. I use them and change every 30 minutes to avoid reakthrough. Debbie Faichney nstitute of Aquaculture niversity of Stirling K -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Rene J Buesa ent: 22 February 2012 13:09 o: histonet@lists.utsouthwestern.edu; Jenny Vega ubject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, lthough dermatitis are well documented. egardless you should use gloves whenever your hands can get in contact with any hemical as a good safety practice. If your colleagues do not want to use loves, that is their prerogative, as is yours to wear them. en? J. --- On Tue, 2/21/12, Jenny Vega wrote: rom: Jenny Vega ubject: [Histonet] Does xylene cause skin cancer? o: histonet@lists.utsouthwestern.edu ate: Tuesday, February 21, 2012, 10:17 PM am asking this because in my job we mount slides by hand, and my coworkers on't like to use gloves because it leaves a residue of latex in the back of the lides. I really don't feel comfortable mounting without gloves because I heard hat xyelene can cause cancer. Some people I know personally has told me that his is not possible, but I read in some places that xylene could a possible arcinogen. I have already gotten contact with xylene in my hands a couple of times and I am orried. Thanks. ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet -- he Sunday Times Scottish University of the Year 2009/2010 The University of tirling is a charity registered in Scotland, number SC 011159. ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* his email and any files transmitted with it are confidential and intended olely for the use of the individual or entity to whom they are addressed. If ou are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual ender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and lthough no computer viruses were detected, The Childrens Hospital at Westmead ccepts no liability for any consequential damage resulting from email ontaining computer viruses. ******************************************************************************** _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From hairlessturtle <@t> gmail.com Thu Feb 23 15:07:12 2012 From: hairlessturtle <@t> gmail.com (E V) Date: Thu Feb 23 15:07:17 2012 Subject: [Histonet] Paraffin Block Shipping Question Message-ID: Hi, I am wanting to send out blocks to different sites across the country. My main concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when considering the above stated concern? Thanks, H From b427297 <@t> aol.com Thu Feb 23 15:23:09 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Feb 23 15:23:18 2012 Subject: [Histonet] Paraffin Block Shipping Question In-Reply-To: References: Message-ID: <8CEC08879D352D2-F8C-573E8@webmail-d021.sysops.aol.com> FedEx overnight with a warning to keep from extreme temps. That should keep them off loading docks. -----Original Message----- From: E V To: histonet Sent: Thu, Feb 23, 2012 3:08 pm Subject: [Histonet] Paraffin Block Shipping Question Hi, am wanting to send out blocks to different sites across the country. My ain concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when onsidering the above stated concern? Thanks, ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Feb 23 15:49:02 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 23 15:49:11 2012 Subject: [Histonet] mAb to Serotonin Message-ID: <4F466DFE.7400.0077.1@harthosp.org> Does anyone have a good monoclonal antibody to Serotonin that they would recommend for diagnostic immunohistochemistry performed on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Margaret.Perry <@t> sdstate.edu Thu Feb 23 15:55:07 2012 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Feb 23 15:55:18 2012 Subject: [Histonet] cytokeratin Lu5 Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE02D904@SDSU-EX03.jacks.local> Has anyone used this antibody on dog? If so which company did you use? Would you be willing to share your protocol? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From shive003 <@t> umn.edu Thu Feb 23 16:06:43 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Feb 23 16:06:47 2012 Subject: [Histonet] cytokeratin Lu5 In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE02D904@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE02D904@SDSU-EX03.jacks.local> Message-ID: Hi Margaret, We have used CK-Lu5 in the past with great results. It stains dog, cat, pig, cow, horse, goat, deer, bird, frog, hedgehog, snake, guinea pig, rat, and bats so far. Use Proteinase digestion for antigen retrieval. We did notice that in recent times the staining intensity diminished with CK-Lu5 on some runs and we had to keep dropping the dilution down to more concentrated levels to achieve the same staining intensity as in the past. Dako's CK-MNF116 is also a great broad spectrum (also stains pretty much the same animals strongly). However, CK-Lu5 stains more hepatocytes than CK-MNF116 does, in my experience. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Lab Univ. of Minnesota St. Paul, MN shive003@umn.edu On Thu, Feb 23, 2012 at 3:55 PM, Perry, Margaret wrote: > Has anyone used this antibody on dog? If so which company did you use? > Would you be willing to share your protocol? > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From fpearsa <@t> clemson.edu Thu Feb 23 16:19:30 2012 From: fpearsa <@t> clemson.edu (Fran Pearsall) Date: Thu Feb 23 16:19:36 2012 Subject: [Histonet] cytology slides Message-ID: <5.0.0.25.2.20120223171427.02cf2068@mail.clemson.edu> Hi every one, I would like to get people's opinions on whether mail-in cytology slides both stained and un-stained should be left out on the counter top or be put in a refrigerator until the next day for the tech to process them? We have always had a policy of leaving them in their cases at room temp. We now have a 'know-it-all' who is now working here and think he knows every thing. I would like a well educated summery to present to my Pathologist. Thanks so much, Fran From rosenfeldtek <@t> hotmail.com Thu Feb 23 16:31:38 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Thu Feb 23 16:32:36 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? In-Reply-To: References: Message-ID: As far as I know Xylene is not a conformed carcinogen. On the other hand the structure is close to that of Benzene which is a confirmed human carcinogen. It's an aromatic hydrocarbon, so why take chances. It makes sense to 1) minimize use. 2) Use fume hoods when possible. 3) Wear PPE--nitrile gloves not latex! http://en.wikipedia.org/wiki/Xylene http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/xylene/health_xyl.html http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/benzene/health_ben.html#_1_6 Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 23 Feb 2012 13:33:00 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Does xylene cause skin cancer? > > What's badly needed is a registry of death certificates of > histotechnologists. The thing I'd want to examine would be the > prevalence of myeloid leukemia and related diseases - known to be > elevated in workers exposed to benzene. If such a correlation were > found, it would mandate eliminating xylene from histology and cytology > labs, and increasing precautions for handling resinous mounting media. > > The American Medical Association maintains such a registry of American > physicians (or used to). It was used to establish that exposure to > ionizing radiation (fluoroscopes and other X-ray equipment) was > correlated with deaths from myeloid leukemia. Pathologists didn't have > any particular problems - here I'd wonder about formaldehyde exposure > and upper airway cancer. Pathologists get more exposure to > formaldehyde than do histotechnologists. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Longf1 <@t> LabCorp.com Thu Feb 23 16:42:38 2012 From: Longf1 <@t> LabCorp.com (Long, Florence) Date: Thu Feb 23 16:45:56 2012 Subject: [Histonet] Paraffin Block Shipping Question In-Reply-To: <8CEC08879D352D2-F8C-573E8@webmail-d021.sysops.aol.com> References: , <8CEC08879D352D2-F8C-573E8@webmail-d021.sysops.aol.com> Message-ID: Fed-Ex is good, but include a cold-pack in with the blocks. A common freezer pack from the local drug store will do it - and is reusable. FL ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor [b427297@aol.com] Sent: Thursday, February 23, 2012 4:23 PM To: hairlessturtle@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block Shipping Question FedEx overnight with a warning to keep from extreme temps. That should keep them off loading docks. -----Original Message----- From: E V To: histonet Sent: Thu, Feb 23, 2012 3:08 pm Subject: [Histonet] Paraffin Block Shipping Question Hi, am wanting to send out blocks to different sites across the country. My ain concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when onsidering the above stated concern? Thanks, ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From 41dmb41 <@t> gmail.com Thu Feb 23 17:32:10 2012 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Thu Feb 23 17:32:34 2012 Subject: [Histonet] Surgipath Millenium VSP3001/Leica Vogel 35396 Slide Writer Message-ID: So apparently this model of slide writer is no longer manufactured, at least from what I can tell. My Leica rep says they don't make it anymore, but I really need to get my hands on one. Normally, I'm not a big fan of this printer for larger volume labs, but the way we make slides here at our immuno lab is perfectly conducive for the specific design of slide writer. I'm wondering if anyone out there in Histoland knows where I can get one new or used. Also, do you know if Leica still sells the ribbons that go with them? Finally, the ideal circumstance would be where we could demo one here; you know how it is getting the funding approved! However, one of our Pathologists has straight up said he'd buy it himself and worry about getting reimbursed for it later. Anyways, please let me know if you can help or point me in the right direction! Thanks, Drew Meyer, HT CSI Laboratories From CIngles <@t> uwhealth.org Thu Feb 23 22:10:08 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Feb 23 22:10:12 2012 Subject: [Histonet] While on the topic of harmful chemicals... Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A7E7@UWHC-MAIL2.uwhis.hosp.wisc.edu> I have been having some health issues lately (understatement) Does anyone know the best formalin resistant disposable gloves? I gross (skin only) under a fume hood but the gloves still come in contact with formalin naturally. I am also (OK, mostly) interested in the health effects involving formalin, Propar, and the alcohols as they pertain to the liver. (No guys, I mean REAGENT alcohols, not the ingestable kind.) :) Any direction I can be pointed in would be great. I am praying I won't have to find a creative way of practicing my beloved career because these chemicals are causing or making worse my health problems. :( Claire From one_angel_secret <@t> yahoo.com Thu Feb 23 23:52:28 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Feb 23 23:52:35 2012 Subject: [Histonet] While on the topic of harmful chemicals... In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A7E7@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <064F1ACBAE8A78469AE2E41D533D87E505A7E7@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: I would still use nitril gloves fir that. Nitril may be a little more expensive but for our department it's the only way to go if safety is practiced. Also I would recommend that you look at the Msds of all reagents you work with. Your department should gave a book. While we are on the subject all should be familiar with the chemicals we work with by looking at the Msds and the protocols which should include proper PPE. Our field of study is a dangerous field. We not only deal with biohazards of fresh tissue blood included we gave mass amounts of chemicals Be safe out there. And Claire I hope you feel better:) Kim :D On Feb 23, 2012, at 11:10 PM, "Ingles Claire " wrote: > I have been having some health issues lately (understatement) Does anyone know the best formalin resistant disposable gloves? I gross (skin only) under a fume hood but the gloves still come in contact with formalin naturally. I am also (OK, mostly) interested in the health effects involving formalin, Propar, and the alcohols as they pertain to the liver. (No guys, I mean REAGENT alcohols, not the ingestable kind.) :) > Any direction I can be pointed in would be great. I am praying I won't have to find a creative way of practicing my beloved career because these chemicals are causing or making worse my health problems. > :( > > Claire > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Fri Feb 24 06:38:25 2012 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Feb 24 06:39:05 2012 Subject: [Histonet] Tissue adhesion Message-ID: Hello, Happy Friday. We are currently running IHC on Ventana XT and Ultra instrumentation. We recently in the past couple of months have seen a rise in tissue section loss. Can anyone share with me their protocol for drying and baking slides prior to IHC automated staining?? We had this problem in the past. We found resolution by not 'over-processing' our small biopsies and treating our slides in certain ways. By that I mean we use adhesion slides, handle and cut all slides with gloves worn, and dip control slides upside down to avoid altering the change on the end of the slides that will hold the patient sample. We currently dry slides for 15 minutes before baking in 60 degree oven for 2 hours. Thank you for any insight you may have. Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From GauchV <@t> mail.amc.edu Fri Feb 24 06:56:46 2012 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Fri Feb 24 06:56:46 2012 Subject: [Histonet] Thank you Message-ID: I just wanted to thank everyone who responded to my VIP 6 inquiry. I really appreciated the assistance and we are setting up a demo. Have a great weekend everyone !! Vicki Gauch AMCH Albany, NY From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Feb 24 07:12:33 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Feb 24 07:13:05 2012 Subject: [Histonet] RE: Tissue adhesion In-Reply-To: References: Message-ID: What kind of slides are you using? I have had bad lot numbers of slides. New lot number cleared up the problem. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa [Melissa.Kuhnla@chsli.org] Sent: Friday, February 24, 2012 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue adhesion Hello, Happy Friday. We are currently running IHC on Ventana XT and Ultra instrumentation. We recently in the past couple of months have seen a rise in tissue section loss. Can anyone share with me their protocol for drying and baking slides prior to IHC automated staining?? We had this problem in the past. We found resolution by not 'over-processing' our small biopsies and treating our slides in certain ways. By that I mean we use adhesion slides, handle and cut all slides with gloves worn, and dip control slides upside down to avoid altering the change on the end of the slides that will hold the patient sample. We currently dry slides for 15 minutes before baking in 60 degree oven for 2 hours. Thank you for any insight you may have. Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Feb 24 08:24:33 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 24 08:24:41 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? In-Reply-To: References: Message-ID: Really, the more important question is, if the lab is unwilling to provide PPE in this case, where else are they lacking in looking after your safety? I've never heard of using xylene without gloves (nitrile or latex). The EH&S department here at Pitt (academic research) would explode with horror if they found out someone was not using PPE with xylene. You need something, at least. Better safe than sorry. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From Wanda.Smith <@t> HCAhealthcare.com Fri Feb 24 08:27:54 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Feb 24 08:28:00 2012 Subject: [Histonet] Paraffin Block Shipping Question In-Reply-To: <8CEC08879D352D2-F8C-573E8@webmail-d021.sysops.aol.com> References: <8CEC08879D352D2-F8C-573E8@webmail-d021.sysops.aol.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA273A82D58B@NADCWPMSGCMS03.hca.corpad.net> Good Morning, During the summer and warm months, we ship blocks with a cold pack in the box. Seems to work fine!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, February 23, 2012 4:23 PM To: hairlessturtle@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block Shipping Question FedEx overnight with a warning to keep from extreme temps. That should keep them off loading docks. -----Original Message----- From: E V To: histonet Sent: Thu, Feb 23, 2012 3:08 pm Subject: [Histonet] Paraffin Block Shipping Question Hi, am wanting to send out blocks to different sites across the country. My ain concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when onsidering the above stated concern? Thanks, ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Fri Feb 24 08:34:55 2012 From: member <@t> linkedin.com (Pamela Marcum via LinkedIn) Date: Fri Feb 24 08:34:57 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1644881948.3262374.1330094095165.JavaMail.app@ela4-bed79.prod> LinkedIn ------------ Pamela Marcum requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Pamela Accept invitation from Pamela Marcum http://www.linkedin.com/e/-gzuoej-gz1bok6x-z/XlF1SInfsMz-GtKrGcmJOZZO1lkFwT3RZom15InljeuxcwwTts/blk/I230048954_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYQdjAUd30McP99bSlBckcMtSkRbPgNdzsUej0UejgLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=1RQTYkCUqGgB81 View invitation from Pamela Marcum http://www.linkedin.com/e/-gzuoej-gz1bok6x-z/XlF1SInfsMz-GtKrGcmJOZZO1lkFwT3RZom15InljeuxcwwTts/blk/I230048954_13/3cNnPgRejwQc30PcAALqnpPbOYWrSlI/svi/?hs=false&tok=3l1ruhLPyGgB81 ------------------------------------------ Why might connecting with Pamela Marcum be a good idea? Pamela Marcum's connections could be useful to you: After accepting Pamela Marcum's invitation, check Pamela Marcum's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From PAMarcum <@t> uams.edu Fri Feb 24 08:40:07 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Feb 24 08:40:10 2012 Subject: [Histonet] Invitation to connect on LinkedIn In-Reply-To: <1644881948.3262374.1330094095165.JavaMail.app@ela4-bed79.prod> References: <1644881948.3262374.1330094095165.JavaMail.app@ela4-bed79.prod> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA323ABA613C@Mail2Node2.ad.uams.edu> Sorry did mean to this on HistoNet!! Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum via LinkedIn Sent: Friday, February 24, 2012 8:35 AM To: David E. Subject: [Histonet] Invitation to connect on LinkedIn LinkedIn ------------ Pamela Marcum requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Pamela Accept invitation from Pamela Marcum http://www.linkedin.com/e/-gzuoej-gz1bok6x-z/XlF1SInfsMz-GtKrGcmJOZZO1lkFwT3RZom15InljeuxcwwTts/blk/I230048954_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYQdjAUd30McP99bSlBckcMtSkRbPgNdzsUej0UejgLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=1RQTYkCUqGgB81 View invitation from Pamela Marcum http://www.linkedin.com/e/-gzuoej-gz1bok6x-z/XlF1SInfsMz-GtKrGcmJOZZO1lkFwT3RZom15InljeuxcwwTts/blk/I230048954_13/3cNnPgRejwQc30PcAALqnpPbOYWrSlI/svi/?hs=false&tok=3l1ruhLPyGgB81 ------------------------------------------ Why might connecting with Pamela Marcum be a good idea? Pamela Marcum's connections could be useful to you: After accepting Pamela Marcum's invitation, check Pamela Marcum's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From rjbuesa <@t> yahoo.com Fri Feb 24 10:42:52 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 24 10:43:26 2012 Subject: [Histonet] Paraffin Block Shipping Question In-Reply-To: Message-ID: <1330101772.18432.YahooMailClassic@web162106.mail.bf1.yahoo.com> I used to send the overnight in one of those Styrofoam containers along with those?freeze and reuse plastic pouches inside. Ren? J. --- On Thu, 2/23/12, E V wrote: From: E V Subject: [Histonet] Paraffin Block Shipping Question To: histonet@lists.utsouthwestern.edu Date: Thursday, February 23, 2012, 4:07 PM Hi, I am wanting to send out blocks to different sites across the country. My main concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when considering the above stated concern? Thanks, H _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 24 10:44:23 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 24 10:44:28 2012 Subject: [Histonet] cytology slides In-Reply-To: <5.0.0.25.2.20120223171427.02cf2068@mail.clemson.edu> Message-ID: <1330101863.11522.YahooMailClassic@web162104.mail.bf1.yahoo.com> Over the counter is OK, even for bone marrow smears. Ren? J. --- On Thu, 2/23/12, Fran Pearsall wrote: From: Fran Pearsall Subject: [Histonet] cytology slides To: histonet@lists.utsouthwestern.edu Date: Thursday, February 23, 2012, 5:19 PM Hi every one,? I would like to get people's opinions on whether mail-in cytology? slides both stained and un-stained should be left out on the counter top or be put in a refrigerator until the next day for the tech to process them?? We have always had a policy of? leaving them in their cases at room temp.? We now have a 'know-it-all'? who is now working here and think he knows every thing.? I would like a well educated summery to present to my Pathologist. Thanks so much,? Fran _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 24 10:47:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 24 10:47:56 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? In-Reply-To: Message-ID: <1330102070.15586.YahooMailClassic@web162106.mail.bf1.yahoo.com> There is an extensive literature linking xylene with some types of cancers, especially "blood cancers",?as well as with birth defects caused by it and many other aromatic compounds. The real solution is to just eliminate it, and that be done?from ALL, and I mean ALL,?the histology phases where it is used now. That is what I did. Ren? J. --- On Thu, 2/23/12, Jerry Ricks wrote: From: Jerry Ricks Subject: [Histonet] Re: Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Thursday, February 23, 2012, 5:31 PM As far as I know Xylene is not a conformed carcinogen.? On the other hand the structure is close to that of Benzene which is a confirmed human carcinogen.? It's an aromatic hydrocarbon, so why take chances.? It makes sense to 1) minimize use. 2) Use fume hoods when possible.? 3) Wear PPE--nitrile gloves not latex! http://en.wikipedia.org/wiki/Xylene http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/xylene/health_xyl.html http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/benzene/health_ben.html#_1_6 Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 23 Feb 2012 13:33:00 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Does xylene cause skin cancer? > > What's badly needed is a registry of death certificates of > histotechnologists. The thing I'd want to examine would be the > prevalence of myeloid leukemia and related diseases - known to be > elevated in workers exposed to benzene. If such a correlation were > found, it would mandate eliminating xylene from histology and cytology > labs, and increasing precautions for handling resinous mounting media. > > The American Medical Association maintains such a registry of American > physicians (or used to). It was used to establish that exposure to > ionizing radiation (fluoroscopes and other X-ray equipment) was > correlated with deaths from myeloid leukemia. Pathologists didn't have > any particular problems - here I'd wonder about formaldehyde exposure > and upper airway cancer. Pathologists get more exposure to > formaldehyde than do histotechnologists. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Feb 24 11:03:03 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Feb 24 11:03:16 2012 Subject: [Histonet] Re: Does xylene cause skin cancer? In-Reply-To: <1330102070.15586.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <1330102070.15586.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB6FEC46@LRGHEXVS1.practice.lrgh.org> I was sent this link a couple of weeks ago, thought I would share it. http://www.darkdaily.com/health-of-pathology-laboratory-technicians-at-risk-from-common-solvents-like-xylene-and-toluene-070511#axzz1hkVgUzSf Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 24, 2012 11:48 AM To: histonet@lists.utsouthwestern.edu; Jerry Ricks Subject: Re: [Histonet] Re: Does xylene cause skin cancer? There is an extensive literature linking xylene with some types of cancers, especially "blood cancers",?as well as with birth defects caused by it and many other aromatic compounds. The real solution is to just eliminate it, and that be done?from ALL, and I mean ALL,?the histology phases where it is used now. That is what I did. Ren? J. --- On Thu, 2/23/12, Jerry Ricks wrote: From: Jerry Ricks Subject: [Histonet] Re: Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Thursday, February 23, 2012, 5:31 PM As far as I know Xylene is not a conformed carcinogen.? On the other hand the structure is close to that of Benzene which is a confirmed human carcinogen.? It's an aromatic hydrocarbon, so why take chances.? It makes sense to 1) minimize use. 2) Use fume hoods when possible.? 3) Wear PPE--nitrile gloves not latex! http://en.wikipedia.org/wiki/Xylene http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/xylene/health_xyl.html http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/benzene/health_ben.html#_1_6 Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 23 Feb 2012 13:33:00 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Does xylene cause skin cancer? > > What's badly needed is a registry of death certificates of > histotechnologists. The thing I'd want to examine would be the > prevalence of myeloid leukemia and related diseases - known to be > elevated in workers exposed to benzene. If such a correlation were > found, it would mandate eliminating xylene from histology and cytology > labs, and increasing precautions for handling resinous mounting media. > > The American Medical Association maintains such a registry of American > physicians (or used to). It was used to establish that exposure to > ionizing radiation (fluoroscopes and other X-ray equipment) was > correlated with deaths from myeloid leukemia. Pathologists didn't have > any particular problems - here I'd wonder about formaldehyde exposure > and upper airway cancer. Pathologists get more exposure to > formaldehyde than do histotechnologists. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rjbuesa <@t> yahoo.com Fri Feb 24 11:05:53 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 24 11:06:28 2012 Subject: [Histonet] Tissue adhesion In-Reply-To: Message-ID: <1330103153.70548.YahooMailClassic@web162105.mail.bf1.yahoo.com> Your drying protocol seems to be adequate. Perhaps your (+) slides are old and in that case they tend to lose their adhesion. Ren? J. --- On Fri, 2/24/12, Kuhnla, Melissa wrote: From: Kuhnla, Melissa Subject: [Histonet] Tissue adhesion To: histonet@lists.utsouthwestern.edu Date: Friday, February 24, 2012, 7:38 AM Hello, Happy Friday. We are currently running IHC on Ventana XT and Ultra instrumentation. We recently in the past couple of months have seen a rise in tissue section loss.? Can anyone share with me their protocol for drying and baking slides prior to IHC automated staining?? We had this problem in the past.? We found resolution by not 'over-processing' our small biopsies and treating our slides in certain ways.? By that I mean we use adhesion slides, handle and cut all slides with gloves worn, and dip control slides upside down to avoid altering the change on the end of the slides that will hold the patient sample.? We currently dry slides for 15 minutes before baking in 60 degree oven for 2 hours. Thank you for any insight you may have. Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail? and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ssegal2 <@t> slu.edu Fri Feb 24 12:23:42 2012 From: ssegal2 <@t> slu.edu (Salomao Segal) Date: Fri Feb 24 12:23:52 2012 Subject: [Histonet] staining of nerve tissue for confocal microscopy Message-ID: Greetings! I am looking for staining methods applicable to thick sections of nervous tissue for confocal microscopy. We would like to stain cell processes and use specialized software for reconstruction of dendrites. We do not have the capability to impale cells and impregnate them with for example lucifer yellow. Golgi? Other methods? Any information would be greatly appreciated. Best wishes and thanks -- Solomon Segal, M.D. Assistant Professor of Anatomy in Surgery Center for Anatomical Science and Education (CASE) Department of Surgery School of Medicine Saint Louis University 1402 South Grand Blvd. Schwitalla Hall - 3rd Floor - M310 Saint Louis, MO, 63104 office: 314 977 8023 laboratory: 314 977 8080 CASE: 314 977 8027 FAX: 314 977 5127 e-mail: ssegal2@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ From modz9636 <@t> gmail.com Fri Feb 24 12:29:49 2012 From: modz9636 <@t> gmail.com (M.O.) Date: Fri Feb 24 12:29:54 2012 Subject: [Histonet] Removing tape Message-ID: Dear All, I am using clear packing tape on mma embedded rabbit femurs with implants. The reasoning for the use of tape is that the implant crumbles when being cut without the tape. I have switched to a new tape that partially comes off with xylene, but does not release from the section on the slide (held with haupts). Does anyone have suggestions for removing the tape - xylene vs toluene vs acetone? At this point, the slides have been in xylene overnight and have not been released from the slides and the other tape took 45mins to lift off. Thank you so much, Merissa From cbrya <@t> lexclin.com Fri Feb 24 12:52:51 2012 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Feb 24 12:52:55 2012 Subject: [Histonet] Removing tape In-Reply-To: References: Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD485@EXCHANGESB> Please reply to all. I am interested in this info. also. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of M.O. Sent: Friday, February 24, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing tape Dear All, I am using clear packing tape on mma embedded rabbit femurs with implants. The reasoning for the use of tape is that the implant crumbles when being cut without the tape. I have switched to a new tape that partially comes off with xylene, but does not release from the section on the slide (held with haupts). Does anyone have suggestions for removing the tape - xylene vs toluene vs acetone? At this point, the slides have been in xylene overnight and have not been released from the slides and the other tape took 45mins to lift off. Thank you so much, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From lblazek <@t> digestivespecialists.com Fri Feb 24 13:49:22 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 24 13:49:26 2012 Subject: [Histonet] fire extinguishers Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com From liz <@t> premierlab.com Fri Feb 24 13:54:40 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Feb 24 13:54:44 2012 Subject: [Histonet] RE: fire extinguishers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC583A@SBS2K8.premierlab.local> Linda We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Feb 24 15:01:58 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 24 15:02:01 2012 Subject: [Histonet] RE: fire extinguishers In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC583A@SBS2K8.premierlab.local> References: <5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> <14E2C6176416974295479C64A11CB9AE011390CC583A@SBS2K8.premierlab.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA0215D@IBMB7Exchange.digestivespecialists.com> Thanks Liz, The guy that was in here today said we should use the Halon or Ansul Cleanguard extinguishers on any piece of equipment to reduce the damage from the ABC extinguishers. I know we have Halon in the server room but I've never heard of having one in the lab itself. He was also talking about a price of $350 - $400. -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Friday, February 24, 2012 2:55 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: fire extinguishers Linda We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Feb 24 15:27:25 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Feb 24 15:27:37 2012 Subject: [Histonet] fire extinguishers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395479063@chimsx08.CHI.catholichealth.net> Use of halon extinguishers.... Do not used in a small or closed space. The way it works is by rapidly depleting oxygen available for the fire. May well do the same for you. Just sayin...... Machinery is replacable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From lpwenk <@t> sbcglobal.net Fri Feb 24 15:30:34 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Feb 24 15:30:36 2012 Subject: Fw: [Histonet] RE: fire extinguishers Message-ID: Be careful with Halon. We at Ford used in in our server rooms, but we had many hundreds of thousands of dollars of servers and a fairly large room. If it is set off, EVERYBODY MUST leave the room. Halon will NOT support life (this is why it works as a fire suppressor). So, along with the Halon, you should have a good way to flush the Halon from the room before returning to it. Lee & Peggy Wenk Actually I'm Lee, not Peggy. -----Original Message----- From: Blazek, Linda Sent: Friday, February 24, 2012 4:01 PM To: 'Elizabeth Chlipala' ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: fire extinguishers Thanks Liz, The guy that was in here today said we should use the Halon or Ansul Cleanguard extinguishers on any piece of equipment to reduce the damage from the ABC extinguishers. I know we have Halon in the server room but I've never heard of having one in the lab itself. He was also talking about a price of $350 - $400. -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Friday, February 24, 2012 2:55 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: fire extinguishers Linda We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Fri Feb 24 15:52:34 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Fri Feb 24 15:52:46 2012 Subject: [Histonet] Grossing techs Message-ID: <197CD0B02A81F94994A285C59C8AE05C083E8B6568@pgnexchange.pathgroup.com> We are looking for help with our grossing. Can you help? You must be CLIA qualified to do the high complexity testing we know as grossing. Times have changed as we used to do this work ourselves, but no more. Two of my staff qualify but I need another. We are a small laboratory in Marin County, just north of SF. Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From pathlocums <@t> gmail.com Fri Feb 24 16:01:09 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Fri Feb 24 16:01:13 2012 Subject: [Histonet] Grossing techs Message-ID: <2522340837944687498@unknownmsgid> Preserve the profession - hire an ASCP Certified PA. Sent from my Windows Phone From: Bruce Gapinski Sent: 2/24/2012 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing techs We are looking for help with our grossing. Can you help? You must be CLIA qualified to do the high complexity testing we know as grossing. Times have changed as we used to do this work ourselves, but no more. Two of my staff qualify but I need another. We are a small laboratory in Marin County, just north of SF. Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson6848 <@t> yahoo.com Fri Feb 24 21:28:55 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Fri Feb 24 21:28:57 2012 Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY Message-ID: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> ? ??? Hi, ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. ?? Hoping to read from you guys asap. You guys are the best. ? Thanks, ? Wilson From rjbuesa <@t> yahoo.com Sat Feb 25 10:34:35 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 25 10:34:40 2012 Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY In-Reply-To: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> Message-ID: <1330187675.4050.YahooMailClassic@web162104.mail.bf1.yahoo.com> To guarantee a "good section" two main issues have to be resolved: "good fixation" leading to "good infiltration". "Good fixation" depends on the slices of tissue being as thin as possible and fixed during the adequate time to assure a complete fixation, especially if you use NBF. If the "good fixation" is achieved, then your processing protocol has to assure a "good infiltration". After that many "good" conditions I am sure you will end with a "good section". Check all the steps in your work flow and check if all steps are as "good" as they should. Ren? J. --- On Fri, 2/24/12, Wilson A wrote: From: Wilson A Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 24, 2012, 10:28 PM ? ??? Hi, ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. ?? Hoping to read from you guys asap. You guys are the best. ? Thanks, ? Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Sat Feb 25 12:42:10 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Feb 25 12:42:16 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 33 In-Reply-To: <4f492207.c8b0ec0a.183c.ffff80eeSMTPIN_ADDED@mx.google.com> References: <4f492207.c8b0ec0a.183c.ffff80eeSMTPIN_ADDED@mx.google.com> Message-ID: From: Wilson A Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY To: "histonet@lists.utsouthwestern.edu" Message-ID: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. I will really, really appreciate if you guys in histoland could suggest some solutions that could stop the problem. Hoping to read from you guys asap. You guys are the best. Thanks, Wilson Wilson, Have you try soften your biopsy tissues in ammonium water (~ 10%) before sectioning? That's seem to work very well for my lab. You can email me for more detail if needed. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org On Sat, Feb 25, 2012 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. staining of nerve tissue for confocal microscopy (Salomao Segal) > ? 2. Removing tape (M.O.) > ? 3. RE: Removing tape (Carol Bryant) > ? 4. fire extinguishers (Blazek, Linda) > ? 5. RE: fire extinguishers (Elizabeth Chlipala) > ? 6. RE: fire extinguishers (Blazek, Linda) > ? 7. RE: fire extinguishers (O'Donnell, Bill) > ? 8. Fw: [Histonet] RE: fire extinguishers (Lee & Peggy Wenk) > ? 9. Grossing techs (Bruce Gapinski) > ?10. RE: Grossing techs (Davide Costanzo) > ?11. PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER ? ? BIOPSY > ? ? ?(Wilson A) > ?12. Re: PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER > ? ? ?BIOPSY (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 24 Feb 2012 12:23:42 -0600 > From: Salomao Segal > Subject: [Histonet] staining of nerve tissue for confocal microscopy > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Greetings! > > I am looking for staining methods applicable to thick sections of nervous > tissue for confocal microscopy. > > We would like to stain cell processes and use specialized software for > reconstruction of dendrites. > > We do not have the capability to impale cells and impregnate them with for > example lucifer yellow. > > Golgi? > > Other methods? > > Any information would be greatly appreciated. > > Best wishes and thanks > > -- > Solomon Segal, M.D. > Assistant Professor of Anatomy in Surgery > Center for Anatomical Science and Education (CASE) > Department of Surgery > School of Medicine > Saint Louis University > > 1402 South Grand Blvd. > Schwitalla Hall - 3rd Floor - M310 > Saint Louis, MO, 63104 > office: 314 977 8023 > laboratory: 314 977 8080 > CASE: 314 977 8027 > FAX: 314 977 5127 > e-mail: ssegal2@slu.edu > > http://medschool.slu.edu/anatomy/ > > http://slu.academia.edu/SolomonSegal > > https://sites.google.com/a/slu.edu/segal-laboratory/ > > https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ > > > ------------------------------ > > Message: 2 > Date: Fri, 24 Feb 2012 10:29:49 -0800 > From: "M.O." > Subject: [Histonet] Removing tape > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Dear All, > > I am using clear packing tape on mma embedded rabbit femurs with implants. > ?The reasoning for the use of tape is that the implant crumbles when being > cut without the tape. ?I have switched to a new tape that partially comes > off with xylene, but does not release from the section on the slide (held > with haupts). ?Does anyone have suggestions for removing the tape - xylene > vs toluene vs acetone? ?At this point, the slides have been in xylene > overnight and have not been released from the slides and the other tape > took 45mins to lift off. > > Thank you so much, > Merissa > > > ------------------------------ > > Message: 3 > Date: Fri, 24 Feb 2012 13:52:51 -0500 > From: Carol Bryant > Subject: RE: [Histonet] Removing tape > To: 'M.O.' , "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD485@EXCHANGESB> > Content-Type: text/plain; charset="us-ascii" > > Please reply to all. ?I am interested in this info. also. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of M.O. > Sent: Friday, February 24, 2012 1:30 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Removing tape > > Dear All, > > I am using clear packing tape on mma embedded rabbit femurs with implants. > ?The reasoning for the use of tape is that the implant crumbles when being > cut without the tape. ?I have switched to a new tape that partially comes > off with xylene, but does not release from the section on the slide (held > with haupts). ?Does anyone have suggestions for removing the tape - xylene > vs toluene vs acetone? ?At this point, the slides have been in xylene > overnight and have not been released from the slides and the other tape > took 45mins to lift off. > > Thank you so much, > Merissa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. ?If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. ?Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > > > > > ------------------------------ > > Message: 4 > Date: Fri, 24 Feb 2012 14:49:22 -0500 > From: "Blazek, Linda" > Subject: [Histonet] fire extinguishers > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="us-ascii" > > Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? ?The company that does our sprinkler system has suggested that we have that kind. > > Thanks, > Linda > > > Our Vision: To be the #1 choice for all your GI services > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > ------------------------------ > > Message: 5 > Date: Fri, 24 Feb 2012 12:54:40 -0700 > From: Elizabeth Chlipala > Subject: [Histonet] RE: fire extinguishers > To: "'Blazek, Linda'" , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<14E2C6176416974295479C64A11CB9AE011390CC583A@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Linda > > We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. ?The Halon ones are for computers and stuff like that. ?They are not inexpensive, they cost about $185.00 each. ?They are liquid that does not damage the computer equipment. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949?office > (303) 682-9060?fax > (303) 881-0763?cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda > Sent: Friday, February 24, 2012 12:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fire extinguishers > > Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? ?The company that does our sprinkler system has suggested that we have that kind. > > Thanks, > Linda > > > Our Vision: To be the #1 choice for all your GI services > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Fri, 24 Feb 2012 16:01:58 -0500 > From: "Blazek, Linda" > Subject: [Histonet] RE: fire extinguishers > To: 'Elizabeth Chlipala' , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<5A2BD13465E061429D6455C8D6B40E39137DA0215D@IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="us-ascii" > > Thanks Liz, > > The guy that was in here today said we should use the Halon or Ansul Cleanguard extinguishers on any piece of equipment to reduce the damage from the ABC extinguishers. ?I know we have Halon in the server room but I've never heard of having one in the lab itself. He was also talking about a price of $350 - $400. > > > -----Original Message----- > From: Elizabeth Chlipala [mailto:liz@premierlab.com] > Sent: Friday, February 24, 2012 2:55 PM > To: Blazek, Linda; histonet@lists.utsouthwestern.edu > Subject: RE: fire extinguishers > > Linda > > We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. ?The Halon ones are for computers and stuff like that. ?They are not inexpensive, they cost about $185.00 each. ?They are liquid that does not damage the computer equipment. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 > (303) 682-3949?office > (303) 682-9060?fax > (303) 881-0763?cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda > Sent: Friday, February 24, 2012 12:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fire extinguishers > > Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? ?The company that does our sprinkler system has suggested that we have that kind. > > Thanks, > Linda > > > Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 24 Feb 2012 14:27:25 -0700 > From: "O'Donnell, Bill" > Subject: RE: [Histonet] fire extinguishers > To: "Blazek, Linda" , > ? ? ? ? > Message-ID: > ? ? ? ?<4940DF6D1C5FDF48931B6966AAEF9395479063@chimsx08.CHI.catholichealth.net> > > Content-Type: text/plain; charset="ISO-8859-1" > > Use of halon extinguishers.... Do not used in a small or closed space. > The way it works is by rapidly depleting oxygen available for the fire. > May well do the same for you. > > Just sayin...... Machinery is replacable. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Friday, February 24, 2012 1:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fire extinguishers > > Does anyone have a Halon or Ansul Clean guard fire extinguisher in the > lab? ?The company that does our sprinkler system has suggested that we > have that kind. > > Thanks, > Linda > > > Our Vision: To be the #1 choice for all your GI services Linda Blazek HT > (ASCP) Manager/Supervisor GI Pathology of Dayton > Phone: (937) 396-2623 > Email: > lblazek@digestivespecialists.com> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > > > ------------------------------ > > Message: 8 > Date: Fri, 24 Feb 2012 16:30:34 -0500 > From: "Lee & Peggy Wenk" > Subject: Fw: [Histonet] RE: fire extinguishers > To: "Histonet" > Message-ID: > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > ? ? ? ?reply-type=original > > Be careful with Halon. ?We at Ford used in in our server rooms, but we had > many hundreds of thousands of dollars of servers and a fairly large room. > If it is set off, EVERYBODY MUST leave the room. ?Halon will NOT support > life > (this is why it works as a fire suppressor). ?So, along with the Halon, you > should have a good way to flush the Halon from the room before returning to > it. > > Lee & Peggy Wenk > > Actually I'm Lee, not Peggy. > > > -----Original Message----- > From: Blazek, Linda > Sent: Friday, February 24, 2012 4:01 PM > To: 'Elizabeth Chlipala' ; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: fire extinguishers > > Thanks Liz, > > The guy that was in here today said we should use the Halon or Ansul > Cleanguard extinguishers on any piece of equipment to reduce the damage from > the ABC extinguishers. ?I know we have Halon in the server room but I've > never heard of having one in the lab itself. He was also talking about a > price of $350 - $400. > > > -----Original Message----- > From: Elizabeth Chlipala [mailto:liz@premierlab.com] > Sent: Friday, February 24, 2012 2:55 PM > To: Blazek, Linda; histonet@lists.utsouthwestern.edu > Subject: RE: fire extinguishers > > Linda > > We do, we have it located where our server is, its only supposed to be used > on our server, we have regular A B C extinguishers in the lab. ?The Halon > ones are for computers and stuff like that. ?They are not inexpensive, they > cost about $185.00 each. ?They are liquid that does not damage the computer > equipment. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308-1592 > (303) 682-3949?office > (303) 682-9060?fax > (303) 881-0763?cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Friday, February 24, 2012 12:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fire extinguishers > > Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? > The company that does our sprinkler system has suggested that we have that > kind. > > Thanks, > Linda > > > Our Vision: To be the #1 choice for all your GI services Linda Blazek HT > (ASCP) Manager/Supervisor GI Pathology of Dayton > Phone: (937) 396-2623 > Email: > lblazek@digestivespecialists.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Fri, 24 Feb 2012 15:52:34 -0600 > From: "Bruce Gapinski" > Subject: [Histonet] Grossing techs > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<197CD0B02A81F94994A285C59C8AE05C083E8B6568@pgnexchange.pathgroup.com> > Content-Type: text/plain; charset=us-ascii > > We are looking for help with our grossing. Can you help? You must be CLIA qualified to do the high complexity testing we know as grossing. Times have changed as we used to do this work ourselves, but no more. Two of my staff qualify but I need another. > We are a small laboratory in Marin County, just north of SF. > Respectfully, > > Bruce Gapinsk HT (ASCP) > Chief Histologist > Marin Medical Laboratories > PathGroup SF > > > ________________________________ > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you > > > ------------------------------ > > Message: 10 > Date: Fri, 24 Feb 2012 14:01:09 -0800 > From: Davide Costanzo > Subject: RE: [Histonet] Grossing techs > To: Bruce Gapinski , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: <2522340837944687498@unknownmsgid> > Content-Type: text/plain; charset=ISO-8859-1 > > Preserve the profession - hire an ASCP Certified PA. > > Sent from my Windows Phone > From: Bruce Gapinski > Sent: 2/24/2012 1:52 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Grossing techs > We are looking for help with our grossing. Can you help? You must be > CLIA qualified to do the high complexity testing we know as grossing. > Times have changed as we used to do this work ourselves, but no more. > Two of my staff qualify but I need another. > We are a small laboratory in Marin County, just north of SF. > Respectfully, > > Bruce Gapinsk HT (ASCP) > Chief Histologist > Marin Medical Laboratories > PathGroup SF > > > ________________________________ > Important Notice: This e-mail is intended for the use of the person to > whom it is addressed and may contain information that is privileged > and confidential. If you are not the intended recipient, any > disclosure, copying, distribution, or use of the contents of this > message is strictly prohibited. If you have received this e-mail in > error, please destroy this message and contact the Security Officer at > PathGroup, Inc immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Fri, 24 Feb 2012 19:28:55 -0800 (PST) > From: Wilson A > Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND > ? ? ? ?LIVER ? BIOPSY > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > ??? Hi, > ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. > ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. > ?? Hoping to read from you guys asap. You guys are the best. > > Thanks, > > Wilson > > ------------------------------ > > Message: 12 > Date: Sat, 25 Feb 2012 08:34:35 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES > ? ? ? ?AND LIVER ? ? ? BIOPSY > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Wilson A > Message-ID: > ? ? ? ?<1330187675.4050.YahooMailClassic@web162104.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > To guarantee a "good section" two main issues have to be resolved: "good fixation" leading to "good infiltration". > "Good fixation" depends on the slices of tissue being as thin as possible and fixed during the adequate time to assure a complete fixation, especially if you use NBF. > If the "good fixation" is achieved, then your processing protocol has to assure a "good infiltration". > After that many "good" conditions I am sure you will end with a "good section". > Check all the steps in your work flow and check if all steps are as "good" as they should. > Ren? J. > > --- On Fri, 2/24/12, Wilson A wrote: > > > From: Wilson A > Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY > To: "histonet@lists.utsouthwestern.edu" > Date: Friday, February 24, 2012, 10:28 PM > > > > ??? Hi, > ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. > ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. > ?? Hoping to read from you guys asap. You guys are the best. > > Thanks, > > Wilson > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 99, Issue 33 > **************************************** From rboen <@t> clearwire.net Sat Feb 25 15:14:40 2012 From: rboen <@t> clearwire.net (Rick and Sue Boen) Date: Sat Feb 25 15:14:50 2012 Subject: [Histonet] Problem getting good sections from Biopsies and Liver Biopsy Message-ID: <77487FD2199042C3A9165B2567CD4B0D@OfficeComputer> Wilson, If you have access, read this article in the Journal of Histotechnology, Volume 34, Issue 2, pp 65-68, "Gastrointestinal Biopsies: More Action and Less "Chatter?". It's a good discussion about improving section quality of small biopsies. Suggestions included: Make sure your processing schedule is right. Face the blocks slowly. Avoid over aggressive facing. Soaking the blocks on ice water after facing. Use only sharp blades. Section slowly at a constant speed. Re-soak the block between levels. Good luck. Rick Boen, HTL (ASCP) St. Luke's Hospital Duluth, Mn From Sruby <@t> 4path.com Sat Feb 25 16:43:44 2012 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Sat Feb 25 16:46:56 2012 Subject: [Histonet] Fire extinguishers for electronic equipment. Message-ID: Halon extinguishers have been phased out. There is now a newer version: Halotron I really don't know the difference chemically. Google : personal fire extinguisher computer equipment it came up with about 3 million hits. With that you should have a huge number of companies which will have them for order. Stephen G. Ruby, MD, MBA, FCAP ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Saturday, February 25, 2012 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 99, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. staining of nerve tissue for confocal microscopy (Salomao Segal) 2. Removing tape (M.O.) 3. RE: Removing tape (Carol Bryant) 4. fire extinguishers (Blazek, Linda) 5. RE: fire extinguishers (Elizabeth Chlipala) 6. RE: fire extinguishers (Blazek, Linda) 7. RE: fire extinguishers (O'Donnell, Bill) 8. Fw: [Histonet] RE: fire extinguishers (Lee & Peggy Wenk) 9. Grossing techs (Bruce Gapinski) 10. RE: Grossing techs (Davide Costanzo) 11. PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY (Wilson A) 12. Re: PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Feb 2012 12:23:42 -0600 From: Salomao Segal Subject: [Histonet] staining of nerve tissue for confocal microscopy To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Greetings! I am looking for staining methods applicable to thick sections of nervous tissue for confocal microscopy. We would like to stain cell processes and use specialized software for reconstruction of dendrites. We do not have the capability to impale cells and impregnate them with for example lucifer yellow. Golgi? Other methods? Any information would be greatly appreciated. Best wishes and thanks -- Solomon Segal, M.D. Assistant Professor of Anatomy in Surgery Center for Anatomical Science and Education (CASE) Department of Surgery School of Medicine Saint Louis University 1402 South Grand Blvd. Schwitalla Hall - 3rd Floor - M310 Saint Louis, MO, 63104 office: 314 977 8023 laboratory: 314 977 8080 CASE: 314 977 8027 FAX: 314 977 5127 e-mail: ssegal2@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ ------------------------------ Message: 2 Date: Fri, 24 Feb 2012 10:29:49 -0800 From: "M.O." Subject: [Histonet] Removing tape To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear All, I am using clear packing tape on mma embedded rabbit femurs with implants. The reasoning for the use of tape is that the implant crumbles when being cut without the tape. I have switched to a new tape that partially comes off with xylene, but does not release from the section on the slide (held with haupts). Does anyone have suggestions for removing the tape - xylene vs toluene vs acetone? At this point, the slides have been in xylene overnight and have not been released from the slides and the other tape took 45mins to lift off. Thank you so much, Merissa ------------------------------ Message: 3 Date: Fri, 24 Feb 2012 13:52:51 -0500 From: Carol Bryant Subject: RE: [Histonet] Removing tape To: 'M.O.' , "histonet@lists.utsouthwestern.edu" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD485@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" Please reply to all. I am interested in this info. also. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of M.O. Sent: Friday, February 24, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing tape Dear All, I am using clear packing tape on mma embedded rabbit femurs with implants. The reasoning for the use of tape is that the implant crumbles when being cut without the tape. I have switched to a new tape that partially comes off with xylene, but does not release from the section on the slide (held with haupts). Does anyone have suggestions for removing the tape - xylene vs toluene vs acetone? At this point, the slides have been in xylene overnight and have not been released from the slides and the other tape took 45mins to lift off. Thank you so much, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ Message: 4 Date: Fri, 24 Feb 2012 14:49:22 -0500 From: "Blazek, Linda" Subject: [Histonet] fire extinguishers To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA0215C@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com ------------------------------ Message: 5 Date: Fri, 24 Feb 2012 12:54:40 -0700 From: Elizabeth Chlipala Subject: [Histonet] RE: fire extinguishers To: "'Blazek, Linda'" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC583A@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Linda We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 24 Feb 2012 16:01:58 -0500 From: "Blazek, Linda" Subject: [Histonet] RE: fire extinguishers To: 'Elizabeth Chlipala' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA0215D@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Thanks Liz, The guy that was in here today said we should use the Halon or Ansul Cleanguard extinguishers on any piece of equipment to reduce the damage from the ABC extinguishers. I know we have Halon in the server room but I've never heard of having one in the lab itself. He was also talking about a price of $350 - $400. -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Friday, February 24, 2012 2:55 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: fire extinguishers Linda We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 24 Feb 2012 14:27:25 -0700 From: "O'Donnell, Bill" Subject: RE: [Histonet] fire extinguishers To: "Blazek, Linda" , Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395479063@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" Use of halon extinguishers.... Do not used in a small or closed space. The way it works is by rapidly depleting oxygen available for the fire. May well do the same for you. Just sayin...... Machinery is replacable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 8 Date: Fri, 24 Feb 2012 16:30:34 -0500 From: "Lee & Peggy Wenk" Subject: Fw: [Histonet] RE: fire extinguishers To: "Histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Be careful with Halon. We at Ford used in in our server rooms, but we had many hundreds of thousands of dollars of servers and a fairly large room. If it is set off, EVERYBODY MUST leave the room. Halon will NOT support life (this is why it works as a fire suppressor). So, along with the Halon, you should have a good way to flush the Halon from the room before returning to it. Lee & Peggy Wenk Actually I'm Lee, not Peggy. -----Original Message----- From: Blazek, Linda Sent: Friday, February 24, 2012 4:01 PM To: 'Elizabeth Chlipala' ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: fire extinguishers Thanks Liz, The guy that was in here today said we should use the Halon or Ansul Cleanguard extinguishers on any piece of equipment to reduce the damage from the ABC extinguishers. I know we have Halon in the server room but I've never heard of having one in the lab itself. He was also talking about a price of $350 - $400. -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Friday, February 24, 2012 2:55 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: fire extinguishers Linda We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, February 24, 2012 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fire extinguishers Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind. Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 24 Feb 2012 15:52:34 -0600 From: "Bruce Gapinski" Subject: [Histonet] Grossing techs To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <197CD0B02A81F94994A285C59C8AE05C083E8B6568@pgnexchange.pathgroup.com> Content-Type: text/plain; charset=us-ascii We are looking for help with our grossing. Can you help? You must be CLIA qualified to do the high complexity testing we know as grossing. Times have changed as we used to do this work ourselves, but no more. Two of my staff qualify but I need another. We are a small laboratory in Marin County, just north of SF. Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 10 Date: Fri, 24 Feb 2012 14:01:09 -0800 From: Davide Costanzo Subject: RE: [Histonet] Grossing techs To: Bruce Gapinski , "histonet@lists.utsouthwestern.edu" Message-ID: <2522340837944687498@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 Preserve the profession - hire an ASCP Certified PA. Sent from my Windows Phone From: Bruce Gapinski Sent: 2/24/2012 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing techs We are looking for help with our grossing. Can you help? You must be CLIA qualified to do the high complexity testing we know as grossing. Times have changed as we used to do this work ourselves, but no more. Two of my staff qualify but I need another. We are a small laboratory in Marin County, just north of SF. Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 24 Feb 2012 19:28:55 -0800 (PST) From: Wilson A Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY To: "histonet@lists.utsouthwestern.edu" Message-ID: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? ??? Hi, ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. ?? Hoping to read from you guys asap. You guys are the best. ? Thanks, ? Wilson ------------------------------ Message: 12 Date: Sat, 25 Feb 2012 08:34:35 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY To: "histonet@lists.utsouthwestern.edu" , Wilson A Message-ID: <1330187675.4050.YahooMailClassic@web162104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 To guarantee a "good section" two main issues have to be resolved: "good fixation" leading to "good infiltration". "Good fixation" depends on the slices of tissue being as thin as possible and fixed during the adequate time to assure a complete fixation, especially if you use NBF. If the "good fixation" is achieved, then your processing protocol has to assure a "good infiltration". After that many "good" conditions I am sure you will end with a "good section". Check all the steps in your work flow and check if all steps are as "good" as they should. Ren? J. --- On Fri, 2/24/12, Wilson A wrote: From: Wilson A Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 24, 2012, 10:28 PM ? ??? Hi, ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. ?? Hoping to read from you guys asap. You guys are the best. ? Thanks, ? Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 99, Issue 33 **************************************** From TeresaJHarris <@t> msn.com Sun Feb 26 01:14:43 2012 From: TeresaJHarris <@t> msn.com (Teresa harris) Date: Sun Feb 26 01:15:05 2012 Subject: [Histonet] Need your help explaining that an automatic microtome does not relieve tediousness with tissue sample preparation. Message-ID: Dear Histonet, I have been employed by the same agency since 1993 as a histotechnologist. I am responsible for histology, cytology and autopsies. I am the only full-time technologist. I even do the medical transcription when necessary. One day I couldn't lift my coffee cup without severe pain. I was diagnosed with severe lateral epicondylitis, right elbow. Personally, I feel that the forceps I used to embed tissue and pick up ribbons of tissue from the microtome and spread that tissue out on the water bath was partially to blame. It could also have been caused by labeling slides and blocks with a marker, scraping excess paraffin off the blocks, lifting gallons of chemicals to change the tissue processor and stainer, lifting bodies on and off the autopsy table, using the cryostat for frozen sections or from using the microtome. Another possible cause would be the logging in of specimens into the computer or filing slides and blocks or staining slides. Anyway, I need your help with a well-reasoned explanation. I will quote the paragraph word for word that requires such explanation to help my cause. "In preparing tissue samples for examination, this Office has been advised that a manual microtome was replaced in 2005 with an automatic microtome which requires the touch of a finger to start. To the extent that you would've been exposed to any tediousness associated with tissue sample preparation, it seems that any upper extremity problem attributable to your exposure would've been while the manual microtome was still in use or within a reasonable period after it was retired. Any delay in any onset of any upper extremity problem associated with this activity requires a well-reasoned explanation." Well it sure took more than pushing a button to make that slide. That much I am sure about. We need to keep up the good work at educating those who have no idea what a histotech does. Thank you for any explanations you want to kindly share. Teresa Harris, HT, HTL(ASCP)QIHC TeresaJHarris@msn.com From acquasi25 <@t> yahoo.com Sun Feb 26 03:05:39 2012 From: acquasi25 <@t> yahoo.com (seth oppong) Date: Sun Feb 26 03:05:43 2012 Subject: [Histonet] TP 1050 tissue processor Message-ID: <1330247139.63969.YahooMailClassic@web65410.mail.ac4.yahoo.com> Hello Histonet, My name is Seth Oppong,a histotech in Ghana,West Africa.A philanthropic histotechnologist in the US is helping me set up a small commercial and research lab of my own.He recently sent me a leica tp 1050 tissue processor which is in fairly good condition.Anytime i switch it on , everything seems to be working fine but anytime , i try to test the retort fill/retort empty or some other functions like retort drain or process, it reads ''error 800'',complete manual wax drain. My problem is how do i complete this manual wax drain?? This machine is kind of new here,so almost all my superiors don't have an idea how it works.Can somebody walk me through a step by step filling of reagent containers,how to check whether the pipes(tubes) are working? Also can somebody link me to a videoclip of the tp 1050 tissue processor usage? i.e how to? load the wax into the wax chambers and how to fill the retort with .Will it matter if i pick the xylene bottle at station say 11 and put it at xylene bottle position say 4? Thank you.I will be glad with as many responses and diverse options as possible. Seth Oppong ClearPath Lab and Diagnostic Center Ghana From lpwenk <@t> sbcglobal.net Sun Feb 26 06:55:59 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Feb 26 06:56:02 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 33 In-Reply-To: References: <4f492207.c8b0ec0a.183c.ffff80eeSMTPIN_ADDED@mx.google.com> Message-ID: <7249FC89997A4E44BCAD44419D50D7CF@HP2010> What is it exactly that they don't like about the biopsies? What type of microtomy errors? Too thick? Chatter? Thick-thin ribbons? Also, are the rest of non-biopsy tissues looking OK? And, are all the tissues being run on the same tissue processor time schedule? We can "guess" at the problem, but we need a little more information, to make certain the Histonet community is giving you the right help. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect upon Beaumont Hospital -----Original Message----- From: Madeleine Huey Sent: Saturday, February 25, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 33 From: Wilson A Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY To: "histonet@lists.utsouthwestern.edu" Message-ID: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. I will really, really appreciate if you guys in histoland could suggest some solutions that could stop the problem. Hoping to read from you guys asap. You guys are the best. Thanks, Wilson Wilson, Have you try soften your biopsy tissues in ammonium water (~ 10%) before sectioning? That's seem to work very well for my lab. You can email me for more detail if needed. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 26 10:43:11 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 26 10:43:15 2012 Subject: [Histonet] Need your help explaining that an automatic microtome does not relieve tediousness with tissue sample preparation. Message-ID: <1330274591.40915.YahooMailClassic@web162106.mail.bf1.yahoo.com> Hi Teresa: What you describe is the result of repetitive actions while completing your histology assignments, and is a cumulative result. The microtome issue surfaced in 1995 (17 years ago!) but I am sure that you started your histology career earlier. Forceps while? embedding and sectioning produce pain in the base of the thumb, but usually not in the elbow. Writing usually affects the wrist. The elbow is mostly affected by the rotatory movement required to move the wheel of a manual microtome. Also it accumulates during the years. Also the work volume is a factor, along with the years during which the repetitive actions have taken place. I have seen articles published on the subject on Laboratory Medicine and other journals (I do not have the references). You could make a Google search with? "ergonomics in histology", "injuries in laboratory personnel" and the like and I am sure you will get some interesting results. At the moment you have to change the way you?complete your tasks, otherwise your symptoms will worsen. Ren? J.? --- On Sun, 2/26/12, Teresa harris wrote: From: Teresa harris Subject: [Histonet] Need your help explaining that an automatic microtome does not relieve tediousness with tissue sample preparation. To: histonet@lists.utsouthwestern.edu Date: Sunday, February 26, 2012, 2:14 AM Dear Histonet, I have been employed by the same agency since 1993 as a histotechnologist.? I am responsible for histology, cytology and autopsies.? I am the only full-time technologist.? I even do the medical transcription when necessary. One day I couldn't lift my coffee cup without severe pain.? I was diagnosed with severe lateral epicondylitis, right elbow.? Personally, I feel that the forceps I used to embed tissue and pick up ribbons of tissue from the microtome and spread that tissue out on the water bath was partially to blame.? It could also have been caused by labeling slides and blocks with a marker, scraping excess paraffin off the blocks, lifting gallons of chemicals to change the tissue processor and stainer, lifting bodies on and off the autopsy table, using the cryostat for frozen sections or from using the microtome.? Another possible cause would be the logging in of specimens into the computer or filing slides and blocks or staining slides. Anyway, I need your help with a well-reasoned explanation.? I will quote the paragraph word for word that requires such explanation to help my cause. "In preparing tissue samples for examination, this Office has been advised that a manual microtome was replaced in 2005 with an automatic microtome which requires the touch of a finger to start.? To the extent that you would've been exposed to any tediousness associated with tissue sample preparation, it seems that any upper extremity problem attributable to your exposure would've been while the manual microtome was still in use or within a reasonable period after it was retired.? Any delay in any onset of any upper extremity problem associated with this activity requires a well-reasoned explanation." Well it sure took more than pushing a button to make that slide.? That much I am sure about.? We need to keep up the good work at educating those who have no idea what a histotech does. Thank you for any explanations you want to kindly share. Teresa Harris, HT, HTL(ASCP)QIHC TeresaJHarris@msn.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 26 10:45:42 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 26 10:45:46 2012 Subject: [Histonet] TP 1050 tissue processor In-Reply-To: <1330247139.63969.YahooMailClassic@web65410.mail.ac4.yahoo.com> Message-ID: <1330274742.39523.YahooMailClassic@web162106.mail.bf1.yahoo.com> Why don't you ask your philanthropic histotechnologist in the US? S/he may know the answer. Ren? J. --- On Sun, 2/26/12, seth oppong wrote: From: seth oppong Subject: [Histonet] TP 1050 tissue processor To: histonet@lists.utsouthwestern.edu Date: Sunday, February 26, 2012, 4:05 AM Hello Histonet, My name is Seth Oppong,a histotech in Ghana,West Africa.A philanthropic histotechnologist in the US is helping me set up a small commercial and research lab of my own.He recently sent me a leica tp 1050 tissue processor which is in fairly good condition.Anytime i switch it on , everything seems to be working fine but anytime , i try to test the retort fill/retort empty or some other functions like retort drain or process, it reads ''error 800'',complete manual wax drain. My problem is how do i complete this manual wax drain?? This machine is kind of new here,so almost all my superiors don't have an idea how it works.Can somebody walk me through a step by step filling of reagent containers,how to check whether the pipes(tubes) are working? Also can somebody link me to a videoclip of the tp 1050 tissue processor usage? i.e how to? load the wax into the wax chambers and how to fill the retort with .Will it matter if i pick the xylene bottle at station say 11 and put it at xylene bottle position say 4? Thank you.I will be glad with as many responses and diverse options as possible. Seth Oppong ClearPath Lab and Diagnostic Center Ghana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhartologist <@t> gmail.com Sun Feb 26 15:52:25 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sun Feb 26 15:52:29 2012 Subject: [Histonet] Scope of Practice Message-ID: Hello everyone! I am currently enrolled in an HT program through IUPUI and am starting to send resumes out as I encroach into the end of the program. I asked my supervisor recently if there was a scope for this field in the world of conservation?! She wasn't sure but recommended this site to me to try to find any answers. It seems as though while biologists/zoologists etc. are out there on the field studying the respective life form they do, there would be a need for histology in there somewhere, but I don't really know how that works in that realm. I'm only familiar with Clinical Labs since that's all I've ever worked in. Any thoughts/ direction? I'd like to get involved in that if possible. I'm looking for work in Calfornia, specifically the bay area if possible. But as long as I can take the BART to where it is that's fine with me also. I wanna make driving to work the last resort, but ya know, gotta work and pay the bills so, if I have to drive, so be it. Thanks ahead of time to those that can shed light on this subject matter for me!!! Sincerely, Bharti Parihar From rjbuesa <@t> yahoo.com Sun Feb 26 16:09:34 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 26 16:09:37 2012 Subject: [Histonet] Scope of Practice In-Reply-To: Message-ID: <1330294174.84382.YahooMailClassic@web162106.mail.bf1.yahoo.com> As you point out, histology is a fundamental tool in human AND veterinary pathology and every clinical AND veterinary laboratory performs histology procedures/tests routinely. In other fields of biology either animal or plant biology it is NOT customary to do histology studies of plants or animals UNLESS the researcher wants to study a specific aspect of the biology of the subject s/he is studying. In this sense it is frequent to study reproduction histology or whole histology of small animals or developmental steps (ontogeny). Other than that it is NOT a given that "natural studies/biology" include histology work, and they are the exception, not the rule. Outside human or veterinary pathology, histology work is not frequent. Ren? J. --- On Sun, 2/26/12, Bharti Parihar wrote: From: Bharti Parihar Subject: [Histonet] Scope of Practice To: histonet@lists.utsouthwestern.edu Date: Sunday, February 26, 2012, 4:52 PM Hello everyone! I am currently enrolled in an HT program through IUPUI and am starting to send resumes out? as I encroach into the end of the program. I asked my supervisor recently if there was a scope for this field in the world of conservation?! She wasn't sure but recommended this site to me to try to find any answers. It seems as though while biologists/zoologists etc. are out there on the field studying the respective life form they do, there would be a need for histology in there somewhere, but I don't really know how that works in that realm. I'm only familiar with Clinical Labs since that's all I've ever worked in. Any thoughts/ direction? I'd like to get involved in that if possible. I'm looking for work in Calfornia, specifically the bay area if possible. But as long as I can take the BART to where it is that's fine with me also. I wanna make driving to work the last resort, but ya know, gotta work and pay the bills so, if I have to drive, so be it. Thanks ahead of time to those that can shed light on this subject matter for me!!! Sincerely, ? ? ???Bharti Parihar _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhartologist <@t> gmail.com Sun Feb 26 16:13:38 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sun Feb 26 16:13:42 2012 Subject: [Histonet] slides for frozens Message-ID: Hi Margaret and Rene, I'm wet behind the ears when it comes to this field, still in Histotech program and have only been in the field as a lab assistant thus far for about 4 years, so I'm trying to understand as much as I can through other's methods. I have a question about your hematoxylin time. Our frozen stain line calls for 30 seconds in hematoxylin, while yours says 5 minutes. We have to try to maintain a 15 minutes or less time frame to get frozens done depending on how many parts we receive of course. Could your hematoxylin time be effecting the sections at all? If it's a fixing issue, we do about 1 minute in 100% and then 1 minute in 95% and then water and then hematoxylin and so on. The only time we experience tissue falling off is with the fragile stuff, like brain, cartilage etc. in which we make sure to use positively charged slides. I don't know the brand of slides we use but I'll try to remember to let you know. Thanks and good luck! -Bharti Parihar From bhartologist <@t> gmail.com Sun Feb 26 16:20:32 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sun Feb 26 16:20:36 2012 Subject: [Histonet] Scope of Practice Message-ID: Wow! Thanks for your response Rene!! I did not know that histology covered such a specific world. So it seems that if a researcher were going about studying ontogeny, that would be a hard area to find and get into. From bhartologist <@t> gmail.com Sun Feb 26 16:39:14 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sun Feb 26 16:39:18 2012 Subject: [Histonet] Samurai Pathologist Message-ID: I'm entering this field young and wet behind the ears. I've almost completed my Histotech program and have worked as a lab assistant for the past 4 years or so. I often hear from my co-workers over in the histology department that "Pathologists and PAs should have to take a class or two in fixation and processing! If they understood more about that we wouldn't receive such poor sections that require reprocessing!" Your thoughts? From dmlaud <@t> gmail.com Sun Feb 26 17:57:59 2012 From: dmlaud <@t> gmail.com (Damien) Date: Sun Feb 26 17:58:04 2012 Subject: [Histonet] Re: Scope of Practice Message-ID: Bharti, I don?t know what your academic background is,aside from the IUPUI program, but I would really encourage you to think ?outside the box? and envision exactly what type of histology career you?d like to have, and in what particular area. There are unique [histology] opportunities out there and you don?t need to limit yourself to a traditional clinical or veterinary diagnostic laboratory (unless you want to). The Bay Area is a place that is potentially brimming with such opportunities. Take the time to do some research on your own, seek out scientists at places like California Academy of Sciences and some of the larger research universities; ask how histology fits into their particular research. Many of the larger natural history museums have dedicated histology facilities. Look for private histology laboratories that may provide services to such facilities/investigators. Maybe you won?t find full-time work doing exactly what you want to do (and may need to augment your income with some traditional clinical/veterinary work), maybe you will; you won?t know what's possible unless you put the work in. A friend once told me,? you?ll make histology work for you?. Those words of wisdom ultimately proved to be true. Good Luck, Damien L. -- Damien Laudier Laudier Histology www.LaudierHistology.com From one_angel_secret <@t> yahoo.com Sun Feb 26 18:04:07 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sun Feb 26 18:04:15 2012 Subject: [Histonet] Scope of Practice In-Reply-To: References: Message-ID: <7FC2F5CC-B9A4-4EB2-B963-769AAEC64195@yahoo.com> Yea, Rene was kind enough to respond. And your right the field is pretty limited. I mean 7 billion plus people on the planet, how many really even understand Histology much less ontogeny? Agh reminds me of my old college days when my professor spoke about " ontogeny recapitulates phylogeny" every chance he could. But then again I lost my ovaries a long time ago. Lol. Good luck with your program. Kim D Sent from my iPhone On Feb 26, 2012, at 5:20 PM, Bharti Parihar wrote: > Wow! Thanks for your response Rene!! I did not know that histology covered > such a specific world. So it seems that if a researcher were going about > studying ontogeny, that would be a hard area to find and get into. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epeters2 <@t> gmu.edu Sun Feb 26 19:32:08 2012 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Sun Feb 26 19:32:12 2012 Subject: [Histonet] Scope of Practice In-Reply-To: <1330294174.84382.YahooMailClassic@web162106.mail.bf1.yahoo.com> References: <1330294174.84382.YahooMailClassic@web162106.mail.bf1.yahoo.com> Message-ID: Hi Bharti, As Rene notes, it is "not a given" that every researcher in conservation or environmental science uses histology in their research, although they should! But, everyone is specialized these days and we can only know and apply so much; however, collaborations with histologists and histopathologists do occur, and many of us have this expertise and use histotechniques in our research. In addition to using histology to study reproduction and development, researchers use it in taxonomy and systematics, physiology, biomarkers, toxicology, and molecular biology studies (of particular value is laser-capture microdissection to obtain targeted cells from FFPE or frozen sections to extract DNA and learn about the genetics of abnormal cells (tumors), microorganisms, or parasites. A recent development in conservation is conservation medicine. Scientists and veterinarians are studying how diseases and anthropogenic changes in the environment are affecting biodiversity and habitats around the world. This requires use of histology to help diagnose diseases and identify etiologic agents, with the hope of managing conservation programs to improve the organisms' and humans' health ("one health"). In the Bay area, check out the University of California at Davis' School of Veterinary Medicine. They have a histology facility. Let me know if you need a contact there (or maybe someone from it will reply to Histonet!). Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030-4444 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epeters2@gmu.edu ----- Original Message ----- From: Rene J Buesa Date: Sunday, February 26, 2012 5:09 pm Subject: Re: [Histonet] Scope of Practice > As you point out, histology is a fundamental tool in human AND > veterinary pathology and every clinical AND veterinary laboratory > performs histology procedures/tests routinely. > In other fields of biology either animal or plant biology it is > NOT customary to do histology studies of plants or animals UNLESS > the researcher wants to study a specific aspect of the biology of > the subject s/he is studying. > In this sense it is frequent to study reproduction histology or > whole histology of small animals or developmental steps (ontogeny). > Other than that it is NOT a given that "natural studies/biology" > include histology work, and they are the exception, not the rule. > Outside human or veterinary pathology, histology work is not frequent. > Ren? J. > > --- On Sun, 2/26/12, Bharti Parihar wrote: > > > From: Bharti Parihar > Subject: [Histonet] Scope of Practice > To: histonet@lists.utsouthwestern.edu > Date: Sunday, February 26, 2012, 4:52 PM > > > Hello everyone! I am currently enrolled in an HT program through > IUPUI and > am starting to send resumes out? as I encroach into the end of the > program.I asked my supervisor recently if there was a scope for > this field in the > world of conservation?! She wasn't sure but recommended this site > to me to > try to find any answers. It seems as though while > biologists/zoologistsetc. are out there on the field studying the > respective life form they do, > there would be a need for histology in there somewhere, but I > don't really > know how that works in that realm. I'm only familiar with Clinical > Labssince that's all I've ever worked in. Any thoughts/ direction? > I'd like to > get involved in that if possible. I'm looking for work in Calfornia, > specifically the bay area if possible. But as long as I can take > the BART > to where it is that's fine with me also. I wanna make driving to > work the > last resort, but ya know, gotta work and pay the bills so, if I > have to > drive, so be it. Thanks ahead of time to those that can shed light > on this > subject matter for me!!! > Sincerely, > ? ? ???Bharti Parihar > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bhartologist <@t> gmail.com Sun Feb 26 20:06:54 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Sun Feb 26 20:07:00 2012 Subject: [Histonet] Scope of Practice Message-ID: Hi Esther, That's incredibly fascinating! I have been wondering if this type of facet exists. That's right in my wheelhouse in terms of where I'd like to take this skill. I plan on finishing my studies with a B.S. and maybe pursue a Master's at California State University East Bay hopefully, as long as everything with admissions goes well. I have studied a bit of zoology in the past which is where I'd really like to take this if possible. It does seem a bit surprising that this particular scope isn't more apparent to those pursuing the field of histology, but like so many things, it's so much about who you know and getting your foot in the door. Until I entered this field a bit more than 4 years ago I had no clue of it's existence, so that just goes to show how things like this unfold. Thank you so much for your insight and offering yourself as a contact! And to everyone else that has shed light on this for me!!! :) -Bharti Parihar From Susan.Walzer <@t> HCAHealthcare.com Mon Feb 27 02:26:33 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Feb 27 02:27:02 2012 Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY In-Reply-To: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> References: <1330140535.4261.YahooMailNeo@web120903.mail.ne1.yahoo.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DE4FBB9B2@FWDCWPMSGCMS09.hca.corpad.net> Overnight processing is usually optimal for average size tissue. Larger tissue may not get optimal processing and biopsies may be a little over processed. It is the nature of tissue processing in a general lab that does not have several processors with different programs. A solution is to rehydrate after facing in the block. I keep soapy water( any liquid soap will work) to which I have added some ammonium hydroxide and pour it on my ice. ( ammonia is great for bloody tissue) for soaking. Remember what Lee Luna said, "rehydrate, rehydrate, rehydrate". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wilson A Sent: Friday, February 24, 2012 10:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY ? ??? Hi, ?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation. ?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem. ?? Hoping to read from you guys asap. You guys are the best. ? Thanks, ? Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marina.Pils <@t> helmholtz-hzi.de Mon Feb 27 06:05:56 2012 From: Marina.Pils <@t> helmholtz-hzi.de (Marina Pils) Date: Mon Feb 27 06:06:07 2012 Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122 Message-ID: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> Dear all, We are trying to establish an anti-GFP staining with rabbit polyclonal A11122 from Invitrogen. We are using formalin fixed, paraffin embedded mouse tissue and want to distinguish between a GFP transgenic mouse strain and a wt mouse. The problem is, we get a strong background in the wt, especially in spleen and lymphnodes, while the staining looks fine in liver. We dilute the antibody 1:400 and use 2 min antigen retrieval in Citrate buffer. Can anyone recommend a protocol? Or any other antibody that is working better? Thanks a lot for your help, Marina ________________________________________ Marina Pils, DVM, PhD Animal experimental unit Helmholtz Centre for Infection Research Inhoffenstr. 7 D 38124 Braunschweig ________________________________ Helmholtz-Zentrum f?r Infektionsforschung GmbH | Inhoffenstra?e 7 | 38124 Braunschweig | www.helmholtz-hzi.de Vorsitzende des Aufsichtsrates: MinDir'in B?rbel Brumme-Bothe, Bundesministerium f?r Bildung und Forschung Stellvertreter: R?diger Eichel, Abteilungsleiter Nieders?chsisches Ministerium f?r Wissenschaft und Kultur Gesch?ftsf?hrung: Prof. Dr. Dirk Heinz; Ulf Richter, MBA Gesellschaft mit beschr?nkter Haftung (GmbH) Sitz der Gesellschaft: Braunschweig Handelsregister: Amtsgericht Braunschweig, HRB 477 From histotalk <@t> yahoo.com Mon Feb 27 08:02:36 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon Feb 27 08:02:44 2012 Subject: [Histonet] HistoTALK Welcomes Carrie Diamond & Brenda Royce Message-ID: <1330351356.71660.YahooMailNeo@web120604.mail.ne1.yahoo.com> Hi NSH'ers - Program #22 of HistoTALK www.HistoTALK.com has a great interview with our Executive Director, Carrie Diamond and Brenda Royce our Membership Manager. Both ladies share with us a huge amount of information and ideas about our "special" day -?Histotechnology Professionals Day on March 10th. Listen at home, at work (quietly) or anywhere you have an Internet connection! ? Yours, Dave From techonebs <@t> comcast.net Mon Feb 27 08:07:09 2012 From: techonebs <@t> comcast.net (Matt Mincer) Date: Mon Feb 27 08:07:23 2012 Subject: [Histonet] Chicago Labs Message-ID: <4F4B8E0D.8080903@comcast.net> Hey Chicagoland Histoneters, We (Tech One Biomedical) are in the process of updating our fliers and catalog. The designer and photographer were hoping to some shots of a real lab. If you are willing to let us do it, please let me know. We will do our best to stay out of the way of your work our could even do the work on the weekend when you are closed. Thanks for your consideration. Best Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From arsenn <@t> hsh.org Mon Feb 27 08:07:22 2012 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Mon Feb 27 08:07:35 2012 Subject: [Histonet] Hire ASCP PA Message-ID: Wish I could hit the "like" button for this email ;) Message: 10 Date: Fri, 24 Feb 2012 14:01:09 -0800 From: Davide Costanzo Subject: RE: [Histonet] Grossing techs To: Bruce Gapinski , "histonet@lists.utsouthwestern.edu" Message-ID: <2522340837944687498@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 Preserve the profession - hire an ASCP Certified PA. Amy Senn, HT Holy Spirit Hospital Histology Laboratory 503 N. 21st Street, Camp Hill, PA 17011 (717) 763-2124 Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From contact <@t> excaliburpathology.com Mon Feb 27 09:19:55 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Feb 27 09:20:03 2012 Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122 In-Reply-To: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> References: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> Message-ID: <1330355995.34858.YahooMailNeo@web5715.biz.mail.ne1.yahoo.com> I use this exact antibody routinely at 1:1000. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Marina Pils To: "'histonet@lists.utsouthwestern.edu'" Sent: Monday, February 27, 2012 6:05 AM Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122 Dear all, We are trying to establish an anti-GFP staining with rabbit polyclonal A11122 from Invitrogen. We are using formalin fixed, paraffin embedded mouse tissue and want to distinguish between a GFP transgenic mouse strain and a wt mouse. The problem is, we get a strong background in the wt, especially in spleen and lymphnodes, while the staining looks fine in liver. We dilute the antibody 1:400 and use 2 min antigen retrieval in Citrate buffer. Can anyone recommend a protocol? Or any other antibody that is working better? Thanks a lot for your help, Marina ________________________________________ Marina Pils, DVM, PhD Animal experimental unit Helmholtz Centre for Infection Research Inhoffenstr. 7 D 38124 Braunschweig ________________________________ Helmholtz-Zentrum f?r Infektionsforschung GmbH | Inhoffenstra?e 7 | 38124 Braunschweig | www.helmholtz-hzi.de Vorsitzende des Aufsichtsrates: MinDir'in B?rbel Brumme-Bothe, Bundesministerium f?r Bildung und Forschung Stellvertreter: R?diger Eichel, Abteilungsleiter Nieders?chsisches Ministerium f?r Wissenschaft und Kultur Gesch?ftsf?hrung: Prof. Dr. Dirk Heinz; Ulf Richter, MBA Gesellschaft mit beschr?nkter Haftung (GmbH) Sitz der Gesellschaft: Braunschweig Handelsregister: Amtsgericht Braunschweig, HRB 477 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Mon Feb 27 09:34:48 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Feb 27 09:34:47 2012 Subject: [Histonet] egfr Message-ID: <000e01ccf565$56f17af0$04d470d0$@com> Good Morning Histonetters I am looking to add EGFR to our antibody list. What clone is everyone using out there in Histoland? Any info would be greatly appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cpyse@x-celllab.com From mtighe <@t> trudeauinstitute.org Mon Feb 27 09:39:41 2012 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Feb 27 09:39:53 2012 Subject: [Histonet] NFAT antibody Message-ID: <108215434A378A4E8246C567E79DD48101D2B8F6@CH1PRD0710MB356.namprd07.prod.outlook.com> Anybody using anti-nFAT antibodies in mouse tissues or tcells? If so, could you recomend a particular antibody? Thanks! Mike From TJohnson <@t> gnf.org Mon Feb 27 11:11:31 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Mon Feb 27 11:11:39 2012 Subject: [Histonet] Re: Removing tape Message-ID: <9F3CFEE76E51B64991C7485270890B4009F03710@EX5.lj.gnf.org> I'm venturing a few guesses here, and would welcome others to do the same. My guess is using acetone might dissolve/soften the plastic from the tape but not remove the adhesive from the top of the tissue section. In thinking about removing adhesive from surfaces where it is unwanted, you sometimes cannot use solvents without compromising the integrity of the surface. Peanut butter or WD40 works well for those types of issues. So I'm thinking maybe WD40 or maybe a soak in mineral oil? I'd probably try mineral oil first, since it's a paraffinic oil. Maybe even warm it? Good luck. Let us know what works for you! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From sbreeden <@t> nmda.nmsu.edu Mon Feb 27 11:31:06 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Feb 27 11:31:22 2012 Subject: [Histonet] Removing coverslipping tape Message-ID: <02C099024072804EA34F5906BAC30A413F1F35@nmdamailsvr.nmda.ad.nmsu.edu> Soak the slide in acetone for 10-15 minutes; it turns to something that looks like a slice of Jell-O and slithers down the slide, leaving cells and stain and structure in perfect order. I usually dip the slide in xylene a couple times to clear the acetone and re-coverslip it. Works like a charm. LOVE the coverslipping tape! I'm down to 20 days until I retire! Not that I'm counting or anything... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From kmerriam2003 <@t> yahoo.com Mon Feb 27 11:34:08 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Feb 27 11:34:15 2012 Subject: [Histonet] fibrin IHC on FFPE mouse tissue Message-ID: <1330364048.88489.YahooMailNeo@web130102.mail.mud.yahoo.com> Is there an antibody out there that will stain for fibrin on FFPE mouse tissues; been searching but haven't found one.? Does everyone just do a special stain when looking for fibrin? ? Kim ? ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From nrutledge <@t> CapeCodHealth.org Mon Feb 27 11:53:52 2012 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Mon Feb 27 11:56:34 2012 Subject: [Histonet] wondering about per diem work in St. Pete, FL Message-ID: <38116B1F59DA314C9DD2D7A0662D78874C6F04@fhcchex2.cchdomain1.capecodhealth.org> Hi all, I'm wondering if anyone knows of any per diem histology jobs in St. Pete area. I am retiring from my current job and will be spending several(winter) months in St. Pete. Would I need a FL license to work per diem? Thanks for any info you can provide. Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From b427297 <@t> aol.com Mon Feb 27 11:56:40 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Mon Feb 27 11:57:01 2012 Subject: [Histonet] Removing coverslipping tape In-Reply-To: <02C099024072804EA34F5906BAC30A413F1F35@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F1F35@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <8CEC3904B2600B6-98C-F928@webmail-m095.sysops.aol.com> Just a note on coverslipping tape - I love it - this is the first place in 40+ years I've used it, and it's good stuff. HOWEVER, I noticed a brown cornflaking artefact on some tissues when I first arrived in this job - and we fixed it. If the last dehydrating step (100%) ethanol has the least little tiny pink from eosin - trace amounts of H20 remain on the slide even through xylene. IF you were using traditional glass coverslips and synthetic mounting medium - this trace H20 is absorbed by the mounting medium. However, the tape is not as forgiving. This trace amount of moisture shows up as brown spots on the tissue. I've proven this theory by removing the tape from affected slides, backing them through xylene to very clean 100%, clear through xylene and remounting with glass. The brown artefact disappears. In this instance it could have been mistaken for melanin on a section of rodent tongue. Jackie O' -----Original Message----- From: Breeden, Sara To: histonet Sent: Mon, Feb 27, 2012 11:32 am Subject: [Histonet] Removing coverslipping tape Soak the slide in acetone for 10-15 minutes; it turns to something that ooks like a slice of Jell-O and slithers down the slide, leaving cells nd stain and structure in perfect order. I usually dip the slide in ylene a couple times to clear the acetone and re-coverslip it. Works ike a charm. LOVE the coverslipping tape! I'm down to 20 days until I retire! Not that I'm counting or nything... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Mon Feb 27 12:00:57 2012 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Mon Feb 27 12:01:06 2012 Subject: [Histonet] Re: Removing tape - clarified Message-ID: <9F3CFEE76E51B64991C7485270890B4009F03766@EX5.lj.gnf.org> Greetings all, My response was to the post sent by Merissa which states: Dear All, I am using clear packing tape on mma embedded rabbit femurs with implants. The reasoning for the use of tape is that the implant crumbles when being cut without the tape. I have switched to a new tape that partially comes off with xylene, but does not release from the section on the slide (held with haupts). Does anyone have suggestions for removing the tape - xylene vs toluene vs acetone? At this point, the slides have been in xylene overnight and have not been released from the slides and the other tape took 45mins to lift off. Thank you so much, Merissa I'm thinking I should have provided this with my email response. My apologies. For those who are still confused, here is my response to the above dilemma: I'm venturing a few guesses here, and would welcome others to do the same. My guess is using acetone might dissolve/soften the plastic from the tape but not remove the adhesive from the top of the tissue section. In thinking about removing adhesive from surfaces where it is unwanted, you sometimes cannot use solvents without compromising the integrity of the surface. Peanut butter or WD40 works well for those types of issues. So I'm thinking maybe WD40 or maybe a soak in mineral oil? I'd probably try mineral oil first, since it's a paraffinic oil. Maybe even warm it? In addition, Diane Sterchi has a JOH article which addresses this in large paraffin block sectioning. She tried different tapes and shows which ones worked better than others. Happy Monday! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From etambutte <@t> centrescientifique.mc Mon Feb 27 12:03:34 2012 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Mon Feb 27 12:03:42 2012 Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 35 Message-ID: <1079744625@s15272523.onlinehome-server.info> Bonjour, Je suis absent du laboratoire jusqu'au lundi 05 mars 2012. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till March 05th 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutté From patjnm <@t> gwumc.edu Mon Feb 27 12:20:43 2012 From: patjnm <@t> gwumc.edu (Joseph Madary) Date: Mon Feb 27 12:34:08 2012 Subject: [Histonet] conservation histotech Message-ID: <4F4B832D.DB55.001F.1@gwumc.edu> Well you can always run your lab in the most green way possible using recyling and reuse management of all materials in the lab. In Md there are a few state labs with coveted positions that only open up through the death or retirement of a tech because they are so rewarding. The closest thing I can thin of are labs that deal with fish and seafood as a means of testing waterways. Hope this helps and good luck in your new field. It has changed since I started in the late 70's(pan embedding, knife sharpening, eating and smoking in the lab)I can only imagine where the field will be in 30 years. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 patjnm@gwumc.edu -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:patjnm@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD From kasaim <@t> mail.nih.gov Mon Feb 27 12:34:11 2012 From: kasaim <@t> mail.nih.gov (Kasai, Miki (NIH/NCI) [E]) Date: Mon Feb 27 12:34:17 2012 Subject: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning Message-ID: We are trouble-shooting cryosectioning mouse lung tissue. Often the lung section tears or breaks apart during sectioning. In the past, if the lung section is proving difficult to section, we take the OCT-embedded tissue and re-embed it back into OCT (basically put fresh OCT into the original mold and then place the OCT block with the tissue back into the mold such that the exposed tissue is covered back with OCT). This is then placed back in our -80?C. When sectioning the next day, the tissue is often easier to section. One person in our lab tried to resolve the problem by brushing a little bit of sterile water onto the tissue when sectioning. This appeared to hydrate the tissue and it sectioned better. However, we weren't sure if this was a good idea or not. Any feedback would be greatly appreciated. For background purposes our lung tissue are processed several ways: 1. Lungs are perfused with PBS, tissue extracted from mouse, placed in PFA/sucrose for several hours and then embedded in OCT. 2. Lungs are perfused with PBS, inflated gently with OCT/PBS (3:1), placed in PFA/sucrose for several hours and then embedded in OCT. 3. Lungs are perfused with PBS, inflated gently with OCT/PBS (3:1), embedded in OCT and frozen by immersion into liquid nitrogen (just the bottom half of the mold is lowered into LN). Much appreciation, Miki Kasai Biologist Pediatric Oncology Branch NCI, NIH CRC, 1W Rm. 1-3-888 10 Center Drive Bethesda, MD 20892 (301) 496-2318 From kmerriam2003 <@t> yahoo.com Mon Feb 27 12:47:03 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Feb 27 12:47:07 2012 Subject: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning In-Reply-To: References: Message-ID: <1330368423.10753.YahooMailNeo@web130101.mail.mud.yahoo.com> As long as you don't need to use them for RNA analysis, the easiest thing to do is just rub your finger (without glove) across the block (very briefly) and then take the section.? This will probably do the trick and hydrate it just enough to make a difference. ? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Kasai, Miki (NIH/NCI) [E]" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 27, 2012 1:34 PM Subject: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning We are trouble-shooting cryosectioning mouse lung tissue.? Often the lung section tears or breaks apart during sectioning.? In the past, if the lung section is proving difficult to section, we take the OCT-embedded tissue and re-embed it back into OCT (basically put fresh OCT into the original mold and then place the OCT block with the tissue back into the mold such that the exposed tissue is covered back with OCT).? This is then placed back in our -80?C.? When sectioning the next day, the tissue is often easier to section. One person in our lab tried to resolve the problem by brushing a little bit of sterile water onto the tissue when sectioning.? This appeared to hydrate the tissue and it sectioned better.? However, we weren't sure if this was a good idea or not.? Any feedback would be greatly appreciated. For background purposes our lung tissue are processed several ways: 1.? Lungs are perfused with PBS, tissue extracted from mouse, placed in PFA/sucrose for several hours and then embedded in OCT. 2.? Lungs are perfused with PBS, inflated gently with OCT/PBS (3:1), placed in PFA/sucrose for several hours and then embedded in OCT. 3.? Lungs are perfused with PBS, inflated gently with OCT/PBS (3:1), embedded in OCT and frozen by immersion into liquid nitrogen (just the bottom half of the mold is lowered into LN). Much appreciation, Miki Kasai Biologist Pediatric Oncology Branch NCI, NIH CRC, 1W Rm. 1-3-888 10 Center Drive Bethesda, MD? 20892 (301) 496-2318 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Mon Feb 27 13:01:06 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Feb 27 13:01:13 2012 Subject: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning In-Reply-To: <4F4BD1AD.9030201@umn.edu> References: <1330368423.10753.YahooMailNeo@web130101.mail.mud.yahoo.com> <4F4BD1AD.9030201@umn.edu> Message-ID: <1330369266.93388.YahooMailNeo@web130105.mail.mud.yahoo.com> Also, make sure that the block sits in the cryostat for a while to acclimate to the temperature.? The cutting temp for lung (correct me if I'm wrong here) is probably about -20; the block should be the same temperature as the cryostat. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Colleen Forster To: Kim Merriam Sent: Monday, February 27, 2012 1:55 PM Subject: Re: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning Yep, the sample is too cold. Rubbing your finger across even with a glove (just linger a bit longer) will help alot. Colleen Forster U of MN On 2/27/2012 12:47 PM, Kim Merriam wrote: > As long as you don't need to use them for RNA analysis, the easiest thing to do is just rub your finger (without glove) across the block (very briefly) and then take the section.? This will probably do the trick and hydrate it just enough to make a difference. >? > Kim > > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > ________________________________ > From: "Kasai, Miki (NIH/NCI) [E]" > To: "histonet@lists.utsouthwestern.edu" > Sent: Monday, February 27, 2012 1:34 PM > Subject: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning > > We are trouble-shooting cryosectioning mouse lung tissue.? Often the lung > section tears or breaks apart during sectioning.? In the past, if the lung > section is proving difficult to section, we take the OCT-embedded tissue and > re-embed it back into OCT (basically put fresh OCT into the original mold > and then place the OCT block with the tissue back into the mold such that > the exposed tissue is covered back with OCT).? This is then placed back in > our -80?C.? When sectioning the next day, the tissue is often easier to > section. > > One person in our lab tried to resolve the problem by brushing a little bit > of sterile water onto the tissue when sectioning.? This appeared to hydrate > the tissue and it sectioned better.? However, we weren't sure if this was a > good idea or not.? Any feedback would be greatly appreciated. > > For background purposes our lung tissue are processed several ways: > > 1.? Lungs are perfused with PBS, tissue extracted from mouse, placed in > PFA/sucrose for several hours and then embedded in OCT. > > 2.? Lungs are perfused with PBS, inflated gently with OCT/PBS (3:1), placed > in PFA/sucrose for several hours and then embedded in OCT. > > 3.? Lungs are perfused with PBS, inflated gently with OCT/PBS (3:1), > embedded in OCT and frozen by immersion into liquid nitrogen (just the > bottom half of the mold is lowered into LN). > > Much appreciation, > Miki Kasai > Biologist > Pediatric Oncology Branch > NCI, NIH > CRC, 1W Rm. 1-3-888 > 10 Center Drive > Bethesda, MD? 20892 > (301) 496-2318 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kcruise <@t> path.wustl.edu Mon Feb 27 15:41:33 2012 From: kcruise <@t> path.wustl.edu (Cruise, Karen) Date: Mon Feb 27 15:41:39 2012 Subject: [Histonet] Parameters for alcohol on Processor Message-ID: Hello Histonetters, Could someone please advise me on established guidelines for changing the alcohol on the processor. We process a very small amount of blocks weekly on our ASP300S. Approximately 20 blocks per week. We have decided to use a hydrometer to test our 70% thru 100% alcohols, rather than base our changing reagents on number of blocks processed. My questions are, does a (1) % point decrease necessitate a change ? What are the parameters for changing each alcohol ? Thanking you in advance, Karen Karen E. Cruise Histologist (Research Technician II) Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Room 2332 St. Louis, MO 63110 (314) 454-8636 Phone (314) 454-5525 Fax kcruise@path.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From amosbrooks <@t> gmail.com Mon Feb 27 16:12:41 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Feb 27 16:12:47 2012 Subject: [Histonet] fibrin IHC on FFPE mouse tissue Message-ID: Hi Kim, I am working on a project just like this right now and we are using Fibrinogen (AbCam ab34269) and PTAH. Drop me a line if you have any questions. Amos On Mon, Feb 27, 2012 at 1:00 PM, wrote: > Message: 20 > Date: Mon, 27 Feb 2012 09:34:08 -0800 (PST) > From: Kim Merriam > Subject: [Histonet] fibrin IHC on FFPE mouse tissue > To: Histonet > Message-ID: > <1330364048.88489.YahooMailNeo@web130102.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Is there an antibody out there that will stain for fibrin on FFPE mouse > tissues; been searching but haven't found one. Does everyone just do a > special stain when looking for fibrin? > > Kim > From koellingr <@t> comcast.net Mon Feb 27 21:12:45 2012 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Feb 27 21:12:49 2012 Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122 In-Reply-To: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> Message-ID: <645260359.65700.1330398765610.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Marina, I agree with Paula Pierce, have used the same anti- GFP antibody on some projects but at 1:500-1:1000 area and with a much longer 8-15 minute retrieval in citrate. Also, I know you know to be aware of "how much" the mice are transfected . In my transfected mice (NOT GFP and in a former life) we might transfect 4 different times and get 4 different efficiencies and 4 different "levels" of expression in 4 different lines. And of course then in your case "the variation with the type of GFP " you are transfecting with might ultimately be seen at varying IHC levels since all GFP antibodies don't see all GFP variants with the same efficiency. Something I did to help ease the pain of working up a stain when working up lacZ (not GFP ) was to use a commercially available Tie2/ LacZ transgenic mouse. With that Tie2 promoter driving LacZ expression only in endothelial cells, you had neg tissue (equivalent to wt) and pos endothelial cells (your transgenic target) right in the very same section. It proved to be an exquisitely sensitive control when doing beta-gal studies. Although I've never used it I understand there is now a Tie2/ GFP transgenic available which should help optimize staining protocols as the expression levels are constant and well characterized and you need only one mouse. Don't know what other gene or transgene target you are after but any given transgene does not incorporate equally all the time as you know. Thats my take on this. Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Marina Pils " To: " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu > Sent: Monday, February 27, 2012 4:05:56 AM Subject: [ Histonet ] anti- GFP staining rabbit polyclonal A11122 Dear all, We are trying to establish an anti- GFP staining with rabbit polyclonal A11122 from Invitrogen . We are using formalin fixed, paraffin embedded mouse tissue and want to distinguish between a GFP transgenic mouse strain and a wt mouse. The problem is, we get a strong background in the wt, especially in spleen and lymphnodes , while the staining looks fine in liver. We dilute the antibody 1:400 and use 2 min antigen retrieval in Citrate buffer. Can anyone recommend a protocol? Or any other antibody that is working better? Thanks a lot for your help, Marina ________________________________________ Marina Pils , DVM , PhD Animal experimental unit Helmholtz Centre for Infection Research Inhoffenstr . 7 D 38124 Braunschweig ________________________________ Helmholtz-Zentrum f?r Infektionsforschung GmbH | Inhoffenstra ?e 7 | 38124 Braunschweig | www .helmholtz-hzi. de Vorsitzende des Aufsichtsrates : MinDir'in B? rbel Brumme-Bothe, Bundesministerium f?r Bildung und Forschung Stellvertreter : R? diger Eichel , Abteilungsleiter Nieders ? chsisches Ministerium f?r Wissenschaft und Kultur Gesch ? ftsf ? hrung : Prof. Dr. Dirk Heinz; Ulf Richter, MBA Gesellschaft mit beschr ? nkter Haftung ( GmbH ) Sitz der Gesellschaft : Braunschweig Handelsregister : Amtsgericht Braunschweig , HRB 477 _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From talulahgosh <@t> gmail.com Tue Feb 28 08:08:10 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Feb 28 08:08:18 2012 Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122 In-Reply-To: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> References: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> Message-ID: We have never been able to get this antibody working with paraffin embedding on mice tissue. It works fine with frozen sections however. We always figured it was the heating that destroyed the GFP antigen. Paula, what fixation protocol and dilution do you use? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From mhale <@t> carisls.com Tue Feb 28 08:26:45 2012 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Feb 28 08:27:01 2012 Subject: [Histonet] HT Position Message-ID: <6F33D8418806044682A391273399860F0C0A3070@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From mw <@t> personifysearch.com Tue Feb 28 08:51:31 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Feb 28 08:51:43 2012 Subject: [Histonet] New Position Alert! - IHC Field Histology Tech South FL - Retained Search Message-ID: <01e735db22533fe026a33634e19c69e9@mail.gmail.com> Good Morning Histonet, Personify has had an IHC Field Histology Tech position open in South FL. The position will ideally be based in Miami or Ft. Lauderdale, and will cover the Southern FL region. We are searching for Histotechs who have experience in IHC and that are open to travel. The position offers a competitive package including Base Salary + Bonus/Commission + Car Allowance, Cell Phone, Laptop, Expenses, Home Office, 401k/Full Benefits. This is a great opportunity to break into the field and have the opportunity for growth in the future. Please contact me directly at mw@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From jclark <@t> pcnm.com Tue Feb 28 10:27:38 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Tue Feb 28 10:29:18 2012 Subject: [Histonet] Cytokeratin AE1/AE3 Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell From brett_connolly <@t> merck.com Tue Feb 28 10:33:03 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Feb 28 10:33:15 2012 Subject: [Histonet] Any Vectra 2 users? Message-ID: Histonetters, We are contemplating upgrading our Vectra multispectral image analysis system to the recently released Vectra 2. Looking for user opinions - i.e. notice any increased speed in HPF scanning, improvements to software, etc. Thanks, Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From LINDA.MARGRAF <@t> childrens.com Tue Feb 28 10:50:33 2012 From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf) Date: Tue Feb 28 10:50:43 2012 Subject: [Histonet] Please welcome Dr. Sandy Cope-Yokoyama to Histonet Message-ID: Dear Histonetters: I wanted to let everyone know that after nearly 20 years at UT Southwestern Medical School ( and 16 years serving as the Histonet administrator), I am leaving the University of Texas. Dr. Sandy Cope-Yokoyama, one of my pathologist colleagues here at the University and at Children's Medical Center in Dallas, has graciously agreed to co-administrate the list with me. I will now use a gmail account to follow the list and deal with day to day member issues (lindamargraf@gmail.com). Dr. Cope will help oversee the administrative functions and answer questions about whether messages are appropriate etc. Both of us will receive the email if you send a message to the "list owner" and I am hoping everything will run smoothly. Thanks for everyone's participation in the list. It is amazing it is still going strong, with 3500 members, after all these years! Cheers, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
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From jqb7 <@t> cdc.gov Tue Feb 28 10:52:36 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Feb 28 10:52:50 2012 Subject: [Histonet] RE: Please welcome Dr. Sandy Cope-Yokoyama to Histonet In-Reply-To: References: Message-ID: Congratulations Linda and thank you Sandy! Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Tuesday, February 28, 2012 11:51 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Please welcome Dr. Sandy Cope-Yokoyama to Histonet Dear Histonetters: I wanted to let everyone know that after nearly 20 years at UT Southwestern Medical School ( and 16 years serving as the Histonet administrator), I am leaving the University of Texas. Dr. Sandy Cope-Yokoyama, one of my pathologist colleagues here at the University and at Children's Medical Center in Dallas, has graciously agreed to co-administrate the list with me. I will now use a gmail account to follow the list and deal with day to day member issues (lindamargraf@gmail.com). Dr. Cope will help oversee the administrative functions and answer questions about whether messages are appropriate etc. Both of us will receive the email if you send a message to the "list owner" and I am hoping everything will run smoothly. Thanks for everyone's participation in the list. It is amazing it is still going strong, with 3500 members, after all these years! Cheers, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
disclosures are prohibited without proper authorization. If you are not the intended recipient, any
disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Feb 28 10:56:36 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Feb 28 10:57:45 2012 Subject: [Histonet] RE: Cytokeratin AE1/AE3 In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> Message-ID: We use the same antibody, but use PK for 5 mins. not HEIR. Very clean Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark [jclark@pcnm.com] Sent: Tuesday, February 28, 2012 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Feb 28 11:02:30 2012 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 28 11:02:39 2012 Subject: [Histonet] RE: Please welcome Dr. Sandy Cope-Yokoyama to Histonet In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640E0E0FB459@CHEXCMS10.one.ads.che.org> Welcome Dr. Sandy! And my continued grateful thanks to you, Dr. Linda, for giving us this much needed forum. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Please note: New email address effective 3/2/12: Joyce.Weems@emoryhealthcare.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Tuesday, February 28, 2012 11:51 To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Please welcome Dr. Sandy Cope-Yokoyama to Histonet Dear Histonetters: I wanted to let everyone know that after nearly 20 years at UT Southwestern Medical School ( and 16 years serving as the Histonet administrator), I am leaving the University of Texas. Dr. Sandy Cope-Yokoyama, one of my pathologist colleagues here at the University and at Children's Medical Center in Dallas, has graciously agreed to co-administrate the list with me. I will now use a gmail account to follow the list and deal with day to day member issues (lindamargraf@gmail.com). Dr. Cope will help oversee the administrative functions and answer questions about whether messages are appropriate etc. Both of us will receive the email if you send a message to the "list owner" and I am hoping everything will run smoothly. Thanks for everyone's participation in the list. It is amazing it is still going strong, with 3500 members, after all these years! Cheers, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From LStadler <@t> cbiolabs.com Tue Feb 28 11:24:32 2012 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Tue Feb 28 11:24:35 2012 Subject: [Histonet] Slide and Cassette Labeling Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC725A8E59@cbiolabs05.CBiolabs.local> Considering Purchasing the Brady Slide and Cassette Labeling System with BSP3 Attachment Unit, the BBP11 Labler, codesoft software and bar code scanner. If anyone out there is using it, I'd love to hear some opinions! Thanks in advance, Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From cpyse <@t> x-celllab.com Tue Feb 28 11:48:09 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Feb 28 11:48:07 2012 Subject: [Histonet] Cytokeratin AE1/AE3 In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> Message-ID: <001b01ccf641$228b1870$67a14950$@com> Joanne What Dako platform are you using, link or 48? On the link I pretreat with PK, the IS series antibody is used with an incubation time of 10 minutes.. On the 48 I pretreat with High pH TRS, the IR antibody is used with an incubation time of 20 minutes. Both systems give me clean staining.(I'm in the process of phasing out the link.) I would try either decreasing the incubation time of the antibody if you stay with the HIER pretreatment or try the enzyme pretreatment with your current protocol. Good luck. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, February 28, 2012 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Feb 28 12:47:59 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Feb 28 12:48:09 2012 Subject: [Histonet] Cytokeratin AE1/AE3 In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3784CB58429@S10MAILD001N1.SH10.lan> Message-ID: <4F4CDB0E.7400.0077.0@harthosp.org> It may be specific labeling of "extra-follicular dendritic cells" that contain low molecular weight cytokeratin and, if your IHC assay is truly optimized, you won't be able to get rid of it. I use the LMW CK mAb clone 5D3 that labels CK8/18 and I see this reactivity all the time. I like it because it serves as an internal positive control. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Joanne Clark 2/28/2012 11:27 AM >>> Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhartologist <@t> gmail.com Tue Feb 28 12:48:25 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Tue Feb 28 12:48:34 2012 Subject: [Histonet] Scope of Practice Message-ID: Damien, Yeah posting my initial comment in regards to this is meant to be "outside the box". While yes, I agree unless I want to, and everyone finds solace in what they do differently, I' dlike to take this field elsewhere than the traditional clinical lab or vet lab. What I do know is that I'd like to work more closely with animals having studied zoology/biology in the past. Other than that I'm eager to learn to expand my horizons. Thanks for the advice on methods of looking into this matter!! It's greatly appreciated!! :) -Bharti From amosbrooks <@t> gmail.com Tue Feb 28 14:18:32 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Feb 28 14:18:36 2012 Subject: [Histonet] Cardiac Myocytes Message-ID: Hi, What would one use to identify cardiac myocytes in mice. I know there are specific antibodies, but I was kinda hoping for something a bit more mundane like desmin. Any ideas? Amos From thisisann <@t> aol.com Tue Feb 28 16:39:01 2012 From: thisisann <@t> aol.com (Ann Angelo) Date: Tue Feb 28 16:39:10 2012 Subject: [Histonet] Agar for cell blocks Message-ID: <8CEC480E69FA4E7-18B8-5262@webmail-m023.sysops.aol.com> Is anyone using an agar for their cell block preparation that they can recommend? ann From lguernsey <@t> ucsd.edu Tue Feb 28 17:56:24 2012 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Tue Feb 28 17:57:08 2012 Subject: [Histonet] Shiny side of a paraffin section Message-ID: As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu From Eric.Hoy <@t> UTSouthwestern.edu Tue Feb 28 19:36:09 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Tue Feb 28 19:36:15 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: <65de5f5e16834fbf8aee0c47e7969160@SWMSHUB2.swmed.org> Message-ID: All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as it > comes off the blade is shiny. I've always accepted it as a fact that the > shiny side always goes down on the water bath, but I've begun to wonder > why. Is there a specific reason why we're all taught to put the shiny side > down? What would the difference be between a 'properly' collected section > and a rebelliously collected shiny-side up section? Does it even matter? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Tue Feb 28 19:46:42 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Tue Feb 28 19:46:50 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: References: Message-ID: <8CEC49B1EA0D961-2004-80A3@webmail-m149.sysops.aol.com> The shiny side (block side) goes on the water so when you do your block to slide comparison the slide WILL match the block. We routinely perform a percentage of slide/block matches for quality control. Some organizations perform 100% slide/block match as a final QC check. Jackie O' -----Original Message----- From: Eric Hoy To: Histonet Sent: Tue, Feb 28, 2012 7:36 pm Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy =============================================== ric S. Hoy, Ph.D., SI(ASCP) linical Associate Professor epartment of Medical Laboratory Sciences he University of Texas Southwestern Medical Center allas, Texas mail: Eric.Hoy@UTSouthwestern.edu ============================================== n 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Tue Feb 28 21:39:39 2012 From: member <@t> linkedin.com (andrea conard via LinkedIn) Date: Tue Feb 28 21:39:43 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1924997892.15714751.1330486779364.JavaMail.app@ela4-bed84.prod> LinkedIn ------------ andrea conard requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - andrea Accept invitation from andrea conard http://www.linkedin.com/e/yvpgd1-gz7th59c-2l/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I232038252_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOdj8UcP0OcP99bRxPq4t7lkFSbPoUc34OdzwMcjoLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=0meiBkUjQXn581 View invitation from andrea conard http://www.linkedin.com/e/yvpgd1-gz7th59c-2l/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I232038252_13/3cNnP8RczwPc38PcAALqnpPbOYWrSlI/svi/?hs=false&tok=2oY7p938wXn581 ------------------------------------------ Why might connecting with andrea conard be a good idea? andrea conard's connections could be useful to you: After accepting andrea conard's invitation, check andrea conard's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From lpwenk <@t> sbcglobal.net Wed Feb 29 03:17:40 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Feb 29 03:17:45 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: References: Message-ID: <9DF525E4CDE04A268D8FA99C0B81425B@HP2010> I think it's more to have consistency, rather than, say, a physical reason. My opinion. Example: - Tech A put the shiny side down on the flotation bath, and picked up the sections on the slide, and did an H&E. - Later in the day, the pathologist needs additional levels or some special stains or IHC on the same block. - If Tech B now cuts the same block and puts shiny side up, the sections would be 180 degrees reversed. So if the pathologist saw the area of concern in lower left quadrant in the original H&E, now it would be in the upper right quadrant. Sort of the same reason when laying out ribbons, it would be nice for the the top of the block be picked up from the ribbon oriented towards the top (frosty) part of the slide. If all techs picked up the ribbon in the same orientation directions, all subsequent recuts would also be in the same direction, regardless of which tech cut the block. (Unless of course you are putting 3 ribbons on the same slide, then the top of the block may be different, but even then, the ribbons are always laid out in the same directions, so that all 3 ribbons of tissue are facing the same direction.) It just makes it easier for the pathologist to find the same area quickly on each section. And for the histotech to check the quality of the staining in specific areas on each slide. Peggy A. Wenk,HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are my own, and do not reflect upon Beaumont Hospital. -----Original Message----- From: Eric Hoy Sent: Tuesday, February 28, 2012 8:36 PM To: Histonet Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as it > comes off the blade is shiny. I've always accepted it as a fact that the > shiny side always goes down on the water bath, but I've begun to wonder > why. Is there a specific reason why we're all taught to put the shiny side > down? What would the difference be between a 'properly' collected section > and a rebelliously collected shiny-side up section? Does it even matter? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmhisto <@t> comcast.net Wed Feb 29 05:53:01 2012 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Wed Feb 29 05:53:23 2012 Subject: [Histonet] Shiny Side of Paraffin Section Message-ID: <434609765.1825280.1330516381477.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Okay, I can't stand this!? Although I? always make sure my ribbon is placed shiny-side-down on the water bath, sometimes the only section?this poor ol' tech is able to get might land upside down.? In that case, I just tell the pathologist the turn the slide over on the 'scope.? Kinda like when they give me a piece of tissue that has to be stuffed into the base mold?? I have to wrap the section around the slide and I let them know they need to turn the slide on its side to see the edges of the tissue.? So far, that's helping to cut down on ?the too-wide tissue sections . Aaaahhh...retirement!? I can sit here in the comfort of my own home and make snide remarks on Histonet at my leisure!? Cowabunga!? The Joy!? And I still have 18 days to go. From TMcNemar <@t> lmhealth.org Wed Feb 29 05:57:39 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Feb 29 05:57:25 2012 Subject: [Histonet] Agar for cell blocks In-Reply-To: <8CEC480E69FA4E7-18B8-5262@webmail-m023.sysops.aol.com> References: <8CEC480E69FA4E7-18B8-5262@webmail-m023.sysops.aol.com> Message-ID: We use soy agar slants that we get from our Micro department. We just melt one as needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, February 28, 2012 5:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Agar for cell blocks Is anyone using an agar for their cell block preparation that they can recommend? ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From leiker <@t> buffalo.edu Wed Feb 29 06:29:34 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Wed Feb 29 06:32:31 2012 Subject: [Histonet] Cardiac Myocytes In-Reply-To: References: Message-ID: We use Cardiac Troponin I or Cardiac Troponin T all the time (pigs). These are also in mice. Regards, Merced ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks [amosbrooks@gmail.com] Sent: Tuesday, February 28, 2012 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cardiac Myocytes Hi, What would one use to identify cardiac myocytes in mice. I know there are specific antibodies, but I was kinda hoping for something a bit more mundane like desmin. Any ideas? Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carstj2002 <@t> hotmail.com Wed Feb 29 06:50:10 2012 From: carstj2002 <@t> hotmail.com (Carmen Maria Garcia Pascual) Date: Wed Feb 29 06:50:18 2012 Subject: [Histonet] autofluorescence and sudan black Message-ID: Good morning!I am Carmen from the University of Valencia. Now I am performing immunofluorescence of mouse decidua. I was having problems with the autofluorescence so I found a sudan black protocol and I probed it, I incubated the tissue 30 min with sudan black after the staining, but I lostes the red signal (alexa 594) so I repited the experiment but incubating before the immunohistochemestry, and want to know if you know if this migth interfere with the antibody attachment to the tissue.thank you very much!!!!!!!!!kind regards!Carmen From talulahgosh <@t> gmail.com Wed Feb 29 08:46:13 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Feb 29 08:46:21 2012 Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122 In-Reply-To: <1330438495.57346.YahooMailNeo@web5713.biz.mail.ne1.yahoo.com> References: <2EE64DCCBDE5E5448D8CF577DBA6C93E02205D@Exchange05.helmholtz-hzi.de> <1330438495.57346.YahooMailNeo@web5713.biz.mail.ne1.yahoo.com> Message-ID: The heating I was referring to was the 60C the paraffin must be melted at to embed the tissue. We also use a vaccuum oven to paraffin embed so maybe that also damages the antigen. We usually leave tissue up to six hours in the paraffin oven before embedding it at room temperature. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Tue, Feb 28, 2012 at 9:14 AM, Paula Pierce < contact@excaliburpathology.com> wrote: > Antigen retrieval will not hurt the GFP antigen in formalin fixed tissue. > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 8901 S. Santa Fe, Suite G > Oklahoma City, OK 73139 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > *From:* Emily Sours > *To:* Marina Pils ; > histonet@lists.utsouthwestern.edu; Paula Pierce < > contact@excaliburpathology.com> > *Sent:* Tuesday, February 28, 2012 8:08 AM > *Subject:* Re: [Histonet] anti-GFP staining rabbit polyclonal A11122 > > We have never been able to get this antibody working with paraffin > embedding on mice tissue. It works fine with frozen sections however. We > always figured it was the heating that destroyed the GFP antigen. Paula, > what fixation protocol and dilution do you use? > > Emily > > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > From rjbuesa <@t> yahoo.com Wed Feb 29 09:25:13 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 29 09:25:22 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: Message-ID: <1330529113.63430.YahooMailClassic@web162105.mail.bf1.yahoo.com> Placing the shiny-side of the section on the water surface you assure that the sections corresponds to the block, of course you cannot turn-around the section. Also it will allow water tension to expand the section better and assures a better adhesion to the slide surface. If the section is in a ribbon you will have to decide which in the ribbon to select and you should not turn around the section. Orientation should not be an issue unless you are always going to section the block in the same way if recuts are needed. Ren? J. --- On Tue, 2/28/12, Lucie Guernsey wrote: From: Lucie Guernsey Subject: [Histonet] Shiny side of a paraffin section To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 28, 2012, 6:56 PM As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Feb 29 09:44:36 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Feb 29 09:44:48 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: References: Message-ID: My mentor, Nick Roman, told me that sections adhere to the slide better if they go on shiny side down. Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says that laying the sections on the water bath or water droplet shiny side down makes it easier to remove creases. Benno Romeiss' MIKROSKOPISCHE TECHNIK and Manfred Gabe's TECHNIQUES HISTOLOGIQUES say that section should be mounted shiny side down without giving a reason. Most other authors do not even mention this matter. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Tuesday, February 28, 2012 6:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shiny side of a paraffin section As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> dsmanitoba.ca Wed Feb 29 10:20:34 2012 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Wed Feb 29 10:20:42 2012 Subject: [Histonet] CAP approved shelf life of dyes & solutions Message-ID: Hi everyone, Does anyone have a list of the shelf life of solutions, chemicals etc. used in Histo staining methods that is acceptable for CAP accreditation & would share the information? We are trying to get a list together but different sources often conflict. I would appreciate any information. Thanks Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From rjbuesa <@t> yahoo.com Wed Feb 29 11:12:01 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 29 11:12:09 2012 Subject: [Histonet] CAP approved shelf life of dyes & solutions In-Reply-To: Message-ID: <1330535521.19215.YahooMailClassic@web162103.mail.bf1.yahoo.com> Such a list does not exist because the shelf life of every reagent (either dry or in solution) is determined by the manufacturer depending on their own specifications or desired turn-over buying/replenishing rate. Ren? J. --- On Wed, 2/29/12, Sharon Allen wrote: From: Sharon Allen Subject: [Histonet] CAP approved shelf life of dyes & solutions To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 29, 2012, 11:20 AM Hi everyone, Does anyone have a list of the shelf life of solutions, chemicals etc. used in Histo staining methods that is acceptable for CAP accreditation & would share the information? We are trying to get a list together but different sources often conflict. I would appreciate any information. Thanks Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -----Inline Attachment Follows----- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information.? Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited.? If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 29 11:12:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 29 11:12:57 2012 Subject: [Histonet] dickeyer Message-ID: <1330535570.73154.yint-ygo-j2me@web162101.mail.bf1.yahoo.com> Learn how to become successfuI from home http://www.countrydiscountgrocery.com/servalicy.php?cybytheme=42 Wed, 29 Feb 2012 18:12:50 ____________ " The castle of Lord Clifford was built at the opening of the gorge, and it commanded an enchanting view of the valley below" (c) cole ainslie From brannon <@t> alliedsearchpartners.com Wed Feb 29 13:53:15 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Feb 29 13:53:30 2012 Subject: [Histonet] Histotechnologist Needed in Fort Myers, FL Message-ID: Allied Search Partners is currently looking for a qualified applicant for a Histotechnologist for a position available in a Fort Myers, FL laboratory. Position: Histotechnologist Schedule: Full time/permanent Summary: Perform a variety of routine and specialized histology techniques and procedures. Embedding, Microtomy, Grossing, Processing, and H&E staining Special Staining Equipment maintenance Requirements: FL Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science Benefits: Competitive salaries, Health, Dental, Life & Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To Apply: Please send resume to brannon@alliedsearchpartners.com -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From sbreeden <@t> nmda.nmsu.edu Wed Feb 29 15:07:29 2012 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Feb 29 15:07:43 2012 Subject: [Histonet] Secret Identity Message-ID: <02C099024072804EA34F5906BAC30A413F1F45@nmdamailsvr.nmda.ad.nmsu.edu> It just occurred to me (after prompting from a friend on Histonet who knows my Secret Identity) that you might not know who nmhisto@comcast is. That would be me. I'm transitioning to my home base and get Histonet there. That explains the strange posting from that person, does it not? But, yes, I'm still working - for now. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 29 15:10:27 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 29 15:10:46 2012 Subject: [Histonet] RE: Secret Identity In-Reply-To: <02C099024072804EA34F5906BAC30A413F1F45@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A413F1F45@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE876@MCINFRWEM003.ucsfmedicalcenter.org> Sally, it was easy - aren't you the only histotech in New Mexico? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 29, 2012 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Secret Identity It just occurred to me (after prompting from a friend on Histonet who knows my Secret Identity) that you might not know who nmhisto@comcast is. That would be me. I'm transitioning to my home base and get Histonet there. That explains the strange posting from that person, does it not? But, yes, I'm still working - for now. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Mielnikowski <@t> leica-microsystems.com Wed Feb 29 16:01:59 2012 From: Diane.Mielnikowski <@t> leica-microsystems.com (Diane.Mielnikowski@leica-microsystems.com) Date: Wed Feb 29 16:02:06 2012 Subject: [Histonet] AUTO: Mielnikowski, Diane is out of the office. (returning 03/01/2012) Message-ID: I am out of the office until 03/01/2012. I am out at a customer site all day and without access to email or voicemail. I will return your message as soon as possible. If this is an emergency, please text my cell at 847-257-3111. Diane Note: This is an automated response to your message "Histonet Digest, Vol 99, Issue 37" sent on 2/29/2012 11:59:17 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From rperez <@t> medsurgpath.com Wed Feb 29 16:15:24 2012 From: rperez <@t> medsurgpath.com (Rolly Perez) Date: Wed Feb 29 16:15:29 2012 Subject: [Histonet] Cryostat Message-ID: Anyone interested in a used, working Cryostat, contact me directly: Leica/Reichert-Jung CRYOCUT 1800 Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. Tigard, OR 503.443.2157 From leiker <@t> buffalo.edu Wed Feb 29 16:16:11 2012 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Wed Feb 29 16:16:43 2012 Subject: [Histonet] autofluorescence and sudan black In-Reply-To: References: Message-ID: It should not interfere. I use Sudan Black a lot. What concentration do you use? Regards, Merced ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual [carstj2002@hotmail.com] Sent: Wednesday, February 29, 2012 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] autofluorescence and sudan black Good morning!I am Carmen from the University of Valencia. Now I am performing immunofluorescence of mouse decidua. I was having problems with the autofluorescence so I found a sudan black protocol and I probed it, I incubated the tissue 30 min with sudan black after the staining, but I lostes the red signal (alexa 594) so I repited the experiment but incubating before the immunohistochemestry, and want to know if you know if this migth interfere with the antibody attachment to the tissue.thank you very much!!!!!!!!!kind regards!Carmen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Feb 29 16:27:33 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Feb 29 16:27:47 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A1DBF6@xmdb02.nch.kids> Consistency, Consistency, Consistency. 1. It is easier to place sections on the water bath with the shiny surface down (MOST times). 2. Staining-wise, for all stains that I have tried, it does not matter (H&E, PAS, Perl's Trichrome to name a few). 3. When you are matching blocks and slides prior to slides leaving the lab, it is difficult to match "rebelliously collected shiny-side up sections" with the corresponding blocks. 4. It is a favourite test of mine to have my staff and trainees look at two slides and tell me the reason for the difference (if there is any). My staff call it "Tony's Migraine Quiz" - one 'properly' collected section and the second a "rebelliously collected shiny-side up section". 4. Microscopically, when comparing special stains with a H&E, it can drive you to distraction when microscopic features do not line up. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, 29 February 2012 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shiny side of a paraffin section As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Feb 29 16:28:37 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Feb 29 16:28:48 2012 Subject: [Histonet] Shiny side of a paraffin section In-Reply-To: References: <65de5f5e16834fbf8aee0c47e7969160@SWMSHUB2.swmed.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A1DC1C@xmdb02.nch.kids> Who wants to look at Cell bottoms all day!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Hoy Sent: Wednesday, 29 February 2012 12:36 PM To: Histonet Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as > it comes off the blade is shiny. I've always accepted it as a fact > that the shiny side always goes down on the water bath, but I've begun > to wonder why. Is there a specific reason why we're all taught to put > the shiny side down? What would the difference be between a 'properly' > collected section and a rebelliously collected shiny-side up section? Does it even matter? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From davidwat <@t> mac.com Wed Feb 29 16:54:42 2012 From: davidwat <@t> mac.com (Watkins David) Date: Wed Feb 29 16:55:04 2012 Subject: [Histonet] Sunquest CoPath Listserver? Message-ID: <625573D1-97A8-4419-A185-2D77D7F0B9AC@mac.com> Does anyone know if there is a Sunquest CoPath listserv? We are upgrading to v.6, and I would like to mine it for information about this new version. thx, David Watkins, MD Department of Pathology Baylor University Medical Center From bjones <@t> personifysearch.com Tue Feb 28 07:31:11 2012 From: bjones <@t> personifysearch.com (Bryan Jones) Date: Mon Mar 12 07:19:41 2012 Subject: [Histonet] Happy Histology Professionals Day! March 10, 2012 Message-ID: <94ff132d012ff6b53da1a864e3895e3f@mail.gmail.com> *Happy Histology Professionals Day from everyone at Personify!* http://www.nsh.org/content/histotechnology-professionals-day If you are considering making a career change or if you are looking to hire someone in the Histology/ IHC world please consider Personify. Personify typically places 50-75 Histology professionals a year and we would love the opportunity to work with you on your hiring needs. Have a great weekend everyone! *Bryan Jones**, CSAM* Practice Director, Laboratory Products Division *Personify* 5020 Weston Parkway Suite 315 Cary, North Carolina 27513 www.personifysearch.com (800) 875-6188 x119 Talent Management | Outsourced Recruitment http://www.linkedin.com/in/bryanjonestalentconsultant