From davidclark10 <@t> comcast.net Sat Dec 1 13:11:19 2012 From: davidclark10 <@t> comcast.net (davidclark10@comcast.net) Date: Sat Dec 1 13:11:26 2012 Subject: [Histonet] Zymed ST 5050 Immuno Stainer Message-ID: <1700306148.727290.1354389079243.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> Hello, Has anyone ever used or knows someone who has used or even perhaps still uses the ST 5050 Immuno stainer? I'm looking to set it up in my Alzheimer's research lab to help off load some of the manual staining we still do... remember I did say 'research'. Thanks and I look forward to reconnecting with my HistoNet buddies! David From daniela.bodemer <@t> mcri.edu.au Sun Dec 2 23:00:25 2012 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Sun Dec 2 23:01:03 2012 Subject: [Histonet] Fixation time Message-ID: <9DF797D618351549B984596F01A1FE1D02ADA6B6@murmx.mcri.edu.au> Hi all, Our tissue processor has been shut down due to a contamination issue and now all the tissues (mice and rat pelves) collected prior to this happening have been sitting in 4% PFA. Some tissues more than a week, when we usually fix for 48 hours. Now we are transferring the tissues to 70% Ethanol and they will sit there until further notice. I am concerned about this process and what it will do to the tissue and would like your thoughts on this. Many thanks, DB Research Assistant ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From kds2041 <@t> bjc.org Mon Dec 3 10:37:02 2012 From: kds2041 <@t> bjc.org (Kevin Selle) Date: Mon Dec 3 10:37:17 2012 Subject: [Histonet] Job opening in St. Louis Message-ID: <50BC80CE020000F9000D7D6C@upbcgwg06> All, We are starting a Night shift at Barnes-Jewish Hospital in St. Louis and are looking to hire two additional histotechs/histotechnologists. Please see the add below. Please note that the add is for HTLs but we are willing to hire HTs. If interested you can apply using our website at www.barnesjewish.org/careers. Surg/Path Histotechnologist F/T/N Barnes-Jewish hospital at Washington University Medical Center is the largest hospital in Missouri and is ranked as one of the nation's top hospitals by U.S. News & World Report. Barnes-Jewish Hospital's staff is composed of full-time academic faculty and community physicians of Washington University School of Medicine, supported by a house staff of residents, interns, fellows and other medical professionals. Recognizing its excellence in nursing care, Barnes-Jewish Hospital was the first adult hospital in Missouri to be certified as a "Magnet Hospital" by the American Nurses Credentialing Center. Role Purpose Responsible for performing routine and complex histology and immunohistochemistry procedures on tissue and related surgical specimens submitted for histological examination. Responsibilities ? Performs quality controls for all equipment and troubleshoots malfunctioning instrumentation as necessary. ? Delivers precise, timely , and accurate histological and immunohistochemistry procedures. ? Understands test principles and procedural aspects related to tissue fixation, processing, embedding, cutting, and staining. Performs automated and manual immunohistochemistry procedures. Minimum Requirements Degree ? Bachelor's Degree - Physical/Life Science/related Experience ? No Experience Supervisor Experience ? No Experience Licenses & Certifications ? HTL-ASCP or equiv Preferred Requirements Experience ? <2 years Supervisor Experience ? < 2 years Benefits Statement Note: not all benefits apply to all openings - Comprehensive medical, dental, life insurance, and disability plan options - Pension Plan - 401(k) plan with company match - Tuition Assistance - Health Care and Dependent Care Reimbursement Accounts - BJC Fitness Center (depending on location) - Earned Time Off Program for vacation, holiday and sick time Legal Statement The above information on this description has been designed to indicate the general nature and level of work performed by employees in this position. It is not designed to contain or be interpreted as an exhaustive list of all responsibilities, duties and qualifications required of employees assigned to this job. Equal Opportunity Employer From rosenfeldtek <@t> hotmail.com Mon Dec 3 13:56:49 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Mon Dec 3 13:56:55 2012 Subject: [Histonet] Disposal of DAB-link to Princeton. In-Reply-To: References: <006301cdcda9$2eddd730$8c998590$@com>, Message-ID: DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry > From: chapcl@yahoo.com > Date: Wed, 28 Nov 2012 13:14:26 -0800 > To: cpyse@x-celllab.com > Subject: Re: [Histonet] chemical disposal > CC: histonet@lists.utsouthwestern.edu > > Chemical hazardous waste. > > Sent from my iPhone > > On Nov 28, 2012, at 12:44 PM, "Cynthia Pyse" wrote: > > > Quick question for Histoland. I am having a debate about DAB disposal. Our > > general manager ( non lab background) insists that the liquid DAB can go > > into a biological hazardous waste. I disagree, it is a chemical and needs to > > be disposed in the chemical hazardous waste. What is everyone else doing to > > dispose of DAB. We are located in NY, I do have those regs. Thanks in > > advance for any and all help. > > > > Cindy > > > > > > > > Cindy Pyse, CLT, HT (ASCP) > > > > Laboratory Manager > > > > X-Cell Laboratories > > > > 20 Northpointe Parkway Suite 100 > > > > Amherst, NY 14228 > > > > 716-250-9235 etx. 232 > > > > e-mail cpyse@x-celllab.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Dec 3 17:48:59 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Dec 3 17:49:04 2012 Subject: [Histonet] Need used refurbished equipment (vendors welcome) Message-ID: <1354578539.28793.YahooMailClassic@web39404.mail.mud.yahoo.com> Hi All- ? I'm working as a manager in a lab and we need some refurbished equipment.? Vendors and refurb contractors please feel free to email me directly-? ? 1. Cryostat; basic but well-sealed.? For surgical use so we don't need all the bells and whistles of a Mohs unit but it will be remote so low maintenance is a plus. THIS IS URGENT--will purchase within two weeks. ? 2.??Paraffin pot - large.? Up to 5 gallons with a small footprint ? 3. Centrifuge: must handle 50ml conical tubes.? For cytology non-gyn fluid prep. ? either email is fine: tkngflght@yahoo.com or admin@fullstaff.org Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From tkngflght <@t> yahoo.com Tue Dec 4 07:18:35 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Dec 4 07:18:44 2012 Subject: [Histonet] Trichrome Message-ID: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> Hi- need help troubleshooting a trichrome.? The stain on the Nexus stainers is inconsistent.? Taking it off the stainer and doing it by hand with a kit has similar results. ? The tissue is NBF fixed needle biopsies on kidney.? There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.? The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... ? Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana ? From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Dec 4 09:58:51 2012 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Dec 4 09:59:02 2012 Subject: [Histonet] test Message-ID: From trathborne <@t> somerset-healthcare.com Tue Dec 4 10:48:48 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Dec 4 10:49:35 2012 Subject: [Histonet] automated microtomes Message-ID: <3AD061FE740D464FAC7BF6B5CFB757071F1F15BD@smcmail02.somerset-healthcare.com> I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni From TMcNemar <@t> lmhealth.org Tue Dec 4 11:53:12 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Dec 4 11:53:06 2012 Subject: [Histonet] Trichrome In-Reply-To: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> Message-ID: Same problem here. Using the Nexus stainer and have also experienced this with the Reticulin as well. Called service today and they elected to send an application specialist instead of a service tech. Will see what happens. Would not have expected to see this when doing the stain by hand.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From wbenton <@t> cua.md Tue Dec 4 11:56:58 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Dec 4 12:01:07 2012 Subject: [Histonet] Trichrome In-Reply-To: References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com>, Message-ID: <0B8979A204680A42B93A52B486088CD92CCF62B768@CUAEXH1.GCU-MD.local> Sounds like you may have a cracked vortex mixer and/or silver in your reagent dispensing line. If the later is the case new tubing will fix this issue and new vortex mixer(s) will fix the issue with mixing. Do you know how to perform a vortex mix test using the service menu functions? If so, place a drop of hematoxylin on a slide and with the lid open you will be able to see if the vortex mixers are blowing the solution counter-clockwise and clockwise. Each mixer does it in a different manner to make sure the reagents are mixed well over the entire slide. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [TMcNemar@lmhealth.org] Sent: Tuesday, December 04, 2012 12:53 PM To: 'Cheryl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Trichrome Same problem here. Using the Nexus stainer and have also experienced this with the Reticulin as well. Called service today and they elected to send an application specialist instead of a service tech. Will see what happens. Would not have expected to see this when doing the stain by hand.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TMcNemar <@t> lmhealth.org Tue Dec 4 12:05:38 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Dec 4 12:05:29 2012 Subject: [Histonet] Trichrome In-Reply-To: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> Message-ID: Forgot to mention that we are also getting areas of zero staining as well. Needle biopsies are beautiful on one end and totally stain free on the other end. Hit and miss... no consistency what so ever. Ran 2 identical slides for MT on opposite sides of the carousel... one small piece of the specimen was beautifully stained on one slide and so overstained on the other as to be unreadable. One had cores that stained from one end to the other and the other slide had cores stained only half way. Routine maintenance has been performed regularly. Seems like an instrument problem to me.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TMcNemar <@t> lmhealth.org Tue Dec 4 12:06:58 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Dec 4 12:06:48 2012 Subject: [Histonet] Trichrome In-Reply-To: <0B8979A204680A42B93A52B486088CD92CCF62B768@CUAEXH1.GCU-MD.local> References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com>, <0B8979A204680A42B93A52B486088CD92CCF62B768@CUAEXH1.GCU-MD.local> Message-ID: Thanks. Will give this a try... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Walter Benton [mailto:wbenton@cua.md] Sent: Tuesday, December 04, 2012 12:57 PM To: Tom McNemar; 'Cheryl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Trichrome Sounds like you may have a cracked vortex mixer and/or silver in your reagent dispensing line. If the later is the case new tubing will fix this issue and new vortex mixer(s) will fix the issue with mixing. Do you know how to perform a vortex mix test using the service menu functions? If so, place a drop of hematoxylin on a slide and with the lid open you will be able to see if the vortex mixers are blowing the solution counter-clockwise and clockwise. Each mixer does it in a different manner to make sure the reagents are mixed well over the entire slide. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [TMcNemar@lmhealth.org] Sent: Tuesday, December 04, 2012 12:53 PM To: 'Cheryl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Trichrome Same problem here. Using the Nexus stainer and have also experienced this with the Reticulin as well. Called service today and they elected to send an application specialist instead of a service tech. Will see what happens. Would not have expected to see this when doing the stain by hand.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa <@t> yahoo.com Tue Dec 4 12:15:57 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 12:16:06 2012 Subject: [Histonet] Fixation time In-Reply-To: <9DF797D618351549B984596F01A1FE1D02ADA6B6@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D02ADA6B6@murmx.mcri.edu.au> Message-ID: <1354644957.17001.YahooMailNeo@web163106.mail.bf1.yahoo.com> You would have been better off leaving the tissues in formalin. You just would have to make sure HIER is correct (had you used your tissues for IHC procedures). Mice and rat tissues which are very lean,?will suffer more in alcohol than in formalin. I would go back to formalin. Ren? J. From: Daniela Bodemer To: Histonet@lists.utsouthwestern.edu Sent: Monday, December 3, 2012 12:00 AM Subject: [Histonet] Fixation time Hi all, Our tissue processor has been shut down due to a contamination issue and now all the tissues (mice and rat pelves) collected prior to this happening have been sitting in 4% PFA. Some tissues more than a week, when we usually fix for 48 hours. Now we are transferring the tissues to 70% Ethanol and they will sit there until further notice. I am concerned about this process and what it will do to the tissue and would like your thoughts on this. Many thanks, DB Research Assistant ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Dec 4 12:19:49 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 12:19:54 2012 Subject: [Histonet] automated microtomes In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB757071F1F15BD@smcmail02.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB757071F1F15BD@smcmail02.somerset-healthcare.com> Message-ID: <1354645189.12776.YahooMailNeo@web163102.mail.bf1.yahoo.com> The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate?wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). I would go with the Leica. Ren? J. From: "Rathborne, Toni" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ebreisch <@t> rchsd.org Tue Dec 4 12:21:48 2012 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Tue Dec 4 12:22:01 2012 Subject: [Histonet] electron microscopy on bone Message-ID: <243B05306324D84E8153380CDABC4F7601145CA2640A@EXBE1.RCHSD.org> I'm hoping someone might be able to guide me to a laboratory which is capable of completing transmission electron microscopy on cortical bone or perhaps would be willing to share a procedure. Our interest is in osteoclast ultrastructure. Thank you. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital San Diego Associate Clinical Professor of Anatomy Dept. of Surgery/Div. of Anatomy UCSD School of Medicine LaJolla, CA ________________________________ NOTICE: This message, including attachments, is for the sole use of the intended recipient(s) and may contain legally protected confidential, proprietary, and/or privileged information. PLEASE DO NOT FORWARD WITHOUT EXPRESS PERMISSION OF THE SENDER. If you are not the intended recipient (or intended recipient's employee or agent), you may not use, copy, disclose, or distribute this message or its attachments. If you believe you have received this message in error, please contact the sender immediately and destroy all copies of the original message and any attachments. Thank you. From rjbuesa <@t> yahoo.com Tue Dec 4 12:26:08 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 12:26:12 2012 Subject: [Histonet] Trichrome In-Reply-To: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> Message-ID: <1354645568.38735.YahooMailNeo@web163106.mail.bf1.yahoo.com> IF you are not successful when the slides are stained on the Nexus?OR manually, while the controls stain well, you can eliminate the instrument as the cause. It is most likely a processing artifact: either incomplete fixation, or overdehydration, or incomplete dewaxing. Look to your protocol instead to your instrument. Ren? J. From: Cheryl To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 8:18 AM Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome.? The stain on the Nexus stainers is inconsistent.? Taking it off the stainer and doing it by hand with a kit has similar results. ? The tissue is NBF fixed needle biopsies on kidney.? There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.? The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... ? Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mhorne <@t> upei.ca Tue Dec 4 12:26:48 2012 From: Mhorne <@t> upei.ca (Margaret Horne) Date: Tue Dec 4 12:27:00 2012 Subject: [Histonet] cost of histology Message-ID: <50BE0828020000D10001BF3B@oes-grpwise.novell.upei.ca> Hello Everyone, I am trying to figure out the cost of materials alone for Embedding, and a simple H&E. We have a multi-user facility where the Researchers do the work, actually their techs ;-) , but I would like to have them replace the supplies used. Slides and blades are easy as they can bring their own, but embedding and staining is trickier. I don't have throughput numbers as the work comes in sporadically. Processing is done in another lab, so I don't need that. Does anyone have some numbers near at hand? Much thanks, Margaret From rjbuesa <@t> yahoo.com Tue Dec 4 12:27:36 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 12:27:46 2012 Subject: [Histonet] electron microscopy on bone In-Reply-To: <243B05306324D84E8153380CDABC4F7601145CA2640A@EXBE1.RCHSD.org> References: <243B05306324D84E8153380CDABC4F7601145CA2640A@EXBE1.RCHSD.org> Message-ID: <1354645656.49373.YahooMailNeo@web163106.mail.bf1.yahoo.com> Try Pella. Ren? J. From: "Breisch, Eric" To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, December 4, 2012 1:21 PM Subject: [Histonet] electron microscopy on bone I'm hoping someone might be able to guide me to a laboratory which is capable of completing transmission electron microscopy on cortical bone or perhaps would be willing to share a procedure. Our interest is in osteoclast ultrastructure. Thank you. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital San Diego Associate Clinical Professor of Anatomy Dept. of Surgery/Div. of Anatomy UCSD? School of Medicine LaJolla, CA ________________________________ NOTICE: This message, including attachments, is for the sole use of the intended recipient(s) and may contain legally protected confidential, proprietary, and/or privileged information. PLEASE DO NOT FORWARD WITHOUT EXPRESS PERMISSION OF THE SENDER. If you are not the intended recipient (or intended recipient's employee or agent), you may not use, copy, disclose, or distribute this message or its attachments. If you believe you have received this message in error, please contact the sender immediately and destroy all copies of the original message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Dec 4 12:29:39 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 12:29:44 2012 Subject: [Histonet] cost of histology In-Reply-To: <50BE0828020000D10001BF3B@oes-grpwise.novell.upei.ca> References: <50BE0828020000D10001BF3B@oes-grpwise.novell.upei.ca> Message-ID: <1354645779.19324.YahooMailNeo@web163103.mail.bf1.yahoo.com> Please go to: http://www.histosearch.com(rene.html) Ren? J. From: Margaret Horne To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 1:26 PM Subject: [Histonet] cost of histology Hello Everyone, I am trying to figure out the cost of materials alone for Embedding, and a simple H&E. We have a multi-user facility where the Researchers do the work, actually their techs ;-) ,? but? I would like to have them replace? the supplies used.? Slides and blades are easy as they can bring their own, but embedding? and staining is trickier.? I don't have throughput numbers as the work comes in sporadically.? Processing is done in another lab, so I don't need that. Does anyone have some numbers near at hand? ? ? ? Much thanks, Margaret _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epeters2 <@t> gmu.edu Tue Dec 4 13:08:55 2012 From: epeters2 <@t> gmu.edu (Esther Peters) Date: Tue Dec 4 13:09:04 2012 Subject: [Histonet] cost of histology In-Reply-To: <1354645779.19324.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <50BE0828020000D10001BF3B@oes-grpwise.novell.upei.ca> <1354645779.19324.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <50BE4A47.8030007@gmu.edu> Hi Rene and Histonet, That should be http://www.histosearch.com/rene.html Great resource, but I am not sure which article discusses how to figure cost of materials? Esther On 12/4/2012 1:29 PM, Rene J Buesa wrote: > Please go to: http://www.histosearch.com(rene.html) > Ren? J. > > From: Margaret Horne > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, December 4, 2012 1:26 PM > Subject: [Histonet] cost of histology > > Hello Everyone, I am trying to figure out the cost of materials alone > for Embedding, and a simple H&E. > > We have a multi-user facility where the Researchers do the work, > actually their techs ;-) , but I would like to have them replace the > supplies used. Slides and blades are easy as they can bring their own, > but embedding and staining is trickier. I don't have throughput > numbers as the work comes in sporadically. Processing is done in > another lab, so I don't need that. > > Does anyone have some numbers near at hand? > > Much thanks, Margaret > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Esther C. Peters, Ph.D. Department of Environmental Science & Policy Biology Program, Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 E-mail: epeters2@gmu.edu From rjbuesa <@t> yahoo.com Tue Dec 4 13:42:14 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 13:42:20 2012 Subject: [Histonet] cost of histology In-Reply-To: <50BE4A47.8030007@gmu.edu> References: <50BE0828020000D10001BF3B@oes-grpwise.novell.upei.ca> <1354645779.19324.YahooMailNeo@web163103.mail.bf1.yahoo.com> <50BE4A47.8030007@gmu.edu> Message-ID: <1354650134.72243.YahooMailNeo@web163105.mail.bf1.yahoo.com> None! I am sending you the costs article under separate cover. Ren? J. From: Esther Peters To: Rene J Buesa Cc: Margaret Horne ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, December 4, 2012 2:08 PM Subject: Re: [Histonet] cost of histology Hi Rene and Histonet, That should be http://www.histosearch.com/rene.html Great resource, but I am not sure which article discusses how to figure cost of materials? Esther On 12/4/2012 1:29 PM, Rene J Buesa wrote: > Please go to: http://www.histosearch.com(rene.html) > Ren? J. > > From: Margaret Horne > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, December 4, 2012 1:26 PM > Subject: [Histonet] cost of histology > > Hello Everyone, I am trying to figure out the cost of materials alone > for Embedding, and a simple H&E. > > We have a multi-user facility where the Researchers do the work, > actually their techs ;-) ,? but? I would like to have them replace? the > supplies used.? Slides and blades are easy as they can bring their own, > but embedding? and staining is trickier.? I don't have throughput > numbers as the work comes in sporadically.? Processing is done in > another lab, so I don't need that. > > Does anyone have some numbers near at hand? > >? ? ? ? Much thanks, Margaret > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Esther C. Peters, Ph.D. Department of Environmental Science & Policy Biology Program, Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 E-mail: epeters2@gmu.edu From micro <@t> superlink.net Tue Dec 4 14:20:21 2012 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Tue Dec 4 14:20:27 2012 Subject: [Histonet] Trichrome References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> <1354645568.38735.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: Right, I bet these biopsies were in fine mesh/holes tissue capsules and air has little or no escape, so tissues or parts of tissues were not in all solutions during fixing/ processing! ----- Original Message ----- From: "Rene J Buesa" To: "Cheryl" ; Sent: Tuesday, December 04, 2012 1:26 PM Subject: Re: [Histonet] Trichrome IF you are not successful when the slides are stained on the Nexus OR manually, while the controls stain well, you can eliminate the instrument as the cause. It is most likely a processing artifact: either incomplete fixation, or overdehydration, or incomplete dewaxing. Look to your protocol instead to your instrument. Ren? J. From: Cheryl To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 8:18 AM Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Tue Dec 4 14:48:24 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Dec 4 14:48:33 2012 Subject: [Histonet] Tissue Processors Message-ID: <50BE6198.6050005@mclean.harvard.edu> Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA From rjbuesa <@t> yahoo.com Tue Dec 4 14:57:09 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 4 14:57:14 2012 Subject: [Histonet] Tissue Processors In-Reply-To: <50BE6198.6050005@mclean.harvard.edu> References: <50BE6198.6050005@mclean.harvard.edu> Message-ID: <1354654629.8337.YahooMailNeo@web163104.mail.bf1.yahoo.com> I always used VIP because of reliability, "toughness" and customer service. Ren? J. From: Tim Wheelock To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 3:48 PM Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Keri.Colwell <@t> inspection.gc.ca Tue Dec 4 16:47:35 2012 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Tue Dec 4 16:47:45 2012 Subject: [Histonet] Veterinary ISH Message-ID: <50BE1B17.C0AD.00BF.1@inspection.gc.ca> Hi Histonetters, I work in an animal research facility and we are trying to establish a working protocol for CISH on bovine tissues. So far we have tried a Dako PNA ISH kit, and had no luck on bovine tonsil tissue (have only tried once so far). We have also been trying to optimize the kit in our lab on human tonsil. We are suing the negative and positive contorl probes provided with the kit, and we are also using Dako kappa/lambda probes. The negative, kappa and lambda look good in the human tissue, but the positive control isn't working out so nicely. Does anyone have any recommendations on kits/probes/protocols to use on animal tissue? We aren't looking for any specific target right now, we just want to get the ISH working. Then we will focus on areas of interest. we have access to any tissue type, but ultimately would want to look at bovine brain. Thanks in advance! Keri From schndar <@t> gmail.com Tue Dec 4 17:26:09 2012 From: schndar <@t> gmail.com (Darius Alex Schneider) Date: Tue Dec 4 17:26:15 2012 Subject: [Histonet] Immunohistochemical detection of cytokines in older frozen sections Message-ID: Dear all, I am trying to detect cytokines in human pancreatic sections from diabetics. The blocks are at least 1y old, the sections are usually fresh. I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin, staining overnight in BSS-saponin with the primary, biotinylated antibody and subsequently tyramid signal amplification and fluorescein based visuatlisation. However, my results are very poor. I am mainly interested in these cytokines: IL1beta IL1r IL2 IL6 IL10 IL17 IL21 TNFalpha IFNgamma Is there somebody out there who is experienced in detecting these cytokines, and could provide me with a comprehensive protocol? Also, who knows what the best positive controls might be? Thank you so much, Darius La Jolla, USA From pathlocums <@t> gmail.com Tue Dec 4 17:39:11 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Dec 4 17:39:19 2012 Subject: [Histonet] Tissue Processors In-Reply-To: <50BE6198.6050005@mclean.harvard.edu> References: <50BE6198.6050005@mclean.harvard.edu> Message-ID: <1628083238193856494@unknownmsgid> The excelsior is getting rave reviews from folks I know that use it. The University of Miami just ordered a few of them from my understanding. Might want to call there and ask their opinion. Personally, I think Sakura is losing ground and for good reason. We use exclusively Thermo branded equipment here. STP420 and Pathcentre. Both are great. Sent from my iPhone On Dec 4, 2012, at 12:49 PM, Tim Wheelock wrote: > Hi Everyone: > > I am currently evaluating three tissue processors. > They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. > > I was wondering if people could give me their critical opinions and preferences on these three machines. > In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. > I currently have a 14 year old Shandon Hypercenter XP. > > Thank you, > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Dec 4 18:40:25 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Dec 4 18:40:28 2012 Subject: [Histonet] Trichrome Message-ID: Hi, It is imperative to make sure the fixation is started immediately and the tissue is fully fixed. Trichrome stains are *notoriously* affected by fixation. I have an image of two lung sections processed simultaneously, embedded and cut at the same time and stained at the same time. The epithelium of one was a nice pink and the other had faded to purple and almost blue. The only difference between them was the duration of fixation. If your control tissue is working and the test tissue is not both on the autostained and manually, I would strongly suspect the fixation of the test tissue. One final test could be to make up fresh solutions and try again, although I would suspect you are likely to get more of the same. All the best, Amos Brooks On Tue, Dec 4, 2012 at 1:00 PM, wrote: > Message: 3 > Date: Tue, 4 Dec 2012 05:18:35 -0800 (PST) > From: Cheryl > Subject: [Histonet] Trichrome > To: histonet@lists.utsouthwestern.edu > Message-ID: > <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi- need help troubleshooting a trichrome. The stain on the Nexus > stainers is inconsistent. Taking it off the stainer and doing it by hand > with a kit has similar results. > > The tissue is NBF fixed needle biopsies on kidney. There is a little > mechanical damage on the edges of the tissue but the collagens are staining > too light and the red overstains. The normal tissue as control is > beautiful -- I know I know--don't force the stain--but it doesn't make > sense to have this much variation from a kit or auto stainer... > > Help? > > From Susan.Walzer <@t> HCAHealthcare.com Wed Dec 5 01:49:17 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Dec 5 01:49:28 2012 Subject: [Histonet] Tissue Processors In-Reply-To: <1354654629.8337.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <50BE6198.6050005@mclean.harvard.edu> <1354654629.8337.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DF82DD6E1@FWDCWPMSGCMS09.hca.corpad.net> VIP always! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2012 3:57 PM To: Tim Wheelock; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Processors I always used VIP because of reliability, "toughness" and customer service. Ren? J. From: Tim Wheelock To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 3:48 PM Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Wed Dec 5 06:10:06 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Dec 5 06:10:17 2012 Subject: [Histonet] automated microtomes In-Reply-To: <1354645189.12776.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <3AD061FE740D464FAC7BF6B5CFB757071F1F15BD@smcmail02.somerset-healthcare.com>, <1354645189.12776.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56C4F0@EXCHANGEMB1.hmc.hurleymc.com> I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). I would go with the Leica. Ren? J. From: "Rathborne, Toni" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Wed Dec 5 06:28:54 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Dec 5 06:29:02 2012 Subject: [Histonet] HistoTALK Message-ID: <1354710534.83756.YahooMailNeo@web121504.mail.ne1.yahoo.com> Hello HistoNetters - ? In case you missed our last two guests on HistoTALK http://www.histotalk.com/, they were Region VII Director, ANDI GRANTHAM and KATHERINE (Kaspar)?KASPRZYK, President of the Arizona Society for Histotechnology. Just wanted to let you know. ? Yours, David From PAMarcum <@t> uams.edu Wed Dec 5 07:00:57 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Dec 5 07:01:34 2012 Subject: [Histonet] Tissue Processors In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DF82DD6E1@FWDCWPMSGCMS09.hca.corpad.net> References: <50BE6198.6050005@mclean.harvard.edu> <1354654629.8337.YahooMailNeo@web163104.mail.bf1.yahoo.com> <4BF03F5404EBDE409AF9232DA74B9DED2DF82DD6E1@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA328E5D5138@Mail2Node2.ad.uams.edu> We have 4 Thermo Excelsiors and prefer them to the VIP we no longer use. It is ease of changing reagents and programming as well as working better for our tissues. We also have Leica ASP300 that is used for only one type of tissue so we no longer need to change it weekly and expose personnel to the fumes of the reagents and possible spills. VIP are great units just not as easy to work with not that other options have become available. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, December 05, 2012 1:49 AM To: rjbuesa@yahoo.com; twheelock@mclean.harvard.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors VIP always! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2012 3:57 PM To: Tim Wheelock; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Processors I always used VIP because of reliability, "toughness" and customer service. Ren? J. From: Tim Wheelock To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 3:48 PM Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From DKBoyd <@t> chs.net Wed Dec 5 07:35:29 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Dec 5 07:35:40 2012 Subject: [Histonet] Tissue Processors In-Reply-To: <50BE6198.6050005@mclean.harvard.edu> References: <50BE6198.6050005@mclean.harvard.edu> Message-ID: <7EAFE982E328304DA6CE2B677BB762467949FE2A@TN001WEXMBX11.US.chs.net> Excelsior> Great workhorse. Very little to no down time. Less tech time needed as reagents dump and rotate automatically. User friendly. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Tuesday, December 04, 2012 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rob <@t> foliobio.com Wed Dec 5 07:44:45 2012 From: rob <@t> foliobio.com (Rob Day) Date: Wed Dec 5 07:44:55 2012 Subject: [Histonet] Immunohistochemical detection of cytokines in older frozen sections In-Reply-To: References: Message-ID: Folio biosciences is testing a process that will rejuvenate older sections to restore degraded antigenicity for ISH. It's designed to help scientists with exactly the problem you are describing Email me if you want to help us test this process using your slides. No charge of course. Rob Day. On Dec 4, 2012, at 6:26 PM, Darius Alex Schneider wrote: > Dear all, > > I am trying to detect cytokines in human pancreatic sections from > diabetics. The blocks are at least 1y old, the sections are usually fresh. > I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin, > staining overnight in BSS-saponin with the primary, biotinylated antibody > and subsequently tyramid signal amplification and fluorescein based > visuatlisation. However, my results are very poor. > I am mainly interested in these cytokines: > IL1beta > IL1r > IL2 > IL6 > IL10 > IL17 > IL21 > TNFalpha > IFNgamma > > Is there somebody out there who is experienced in detecting these > cytokines, and could provide me with a comprehensive protocol? Also, who > knows what the best positive controls might be? > > Thank you so much, > > Darius > La Jolla, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. From Rebecca.Riesen <@t> hma.com Wed Dec 5 07:47:44 2012 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Wed Dec 5 07:47:52 2012 Subject: [Histonet] RE: Histonet Digest, Vol 109, Issue 5 In-Reply-To: <19740412024625.9F310513C812638E@mx2.hma.com> References: <19740412024625.9F310513C812638E@mx2.hma.com> Message-ID: I am in love with the Excelsior from the standpoint of hands off daily maintenance. Waste paraffin is pumped into a container with a liner, you let it solidify and remove waste in the bag for easy disposal. Pour a couple bags of paraffin pellets in and walk away. Take a bottle of waste alcohol or xylene the instrument has pumped into off and replace it with a new bottle. Excelsior will aspirate the new fluid on the next run. You don't even have to pour into another container. The Excelsior takes the fluids right out of the manufacturer's bottle. It is so simple! If you can read, that's all you need. The screen prompts everything. I also find my solutions last longer. ------------------------------ Message: 18 Date: Tue, 4 Dec 2012 15:39:11 -0800 From: Davide Costanzo Subject: Re: [Histonet] Tissue Processors To: Tim Wheelock Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1628083238193856494@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 The excelsior is getting rave reviews from folks I know that use it. The University of Miami just ordered a few of them from my understanding. Might want to call there and ask their opinion. Personally, I think Sakura is losing ground and for good reason. We use exclusively Thermo branded equipment here. STP420 and Pathcentre. Both are great. From mcauliff <@t> umdnj.edu Wed Dec 5 08:51:53 2012 From: mcauliff <@t> umdnj.edu (Geoff) Date: Wed Dec 5 08:52:05 2012 Subject: [Histonet] Fixation time In-Reply-To: <9DF797D618351549B984596F01A1FE1D02ADA6B6@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D02ADA6B6@murmx.mcri.edu.au> Message-ID: <50BF5F89.20200@umdnj.edu> I agree with Rene. Geoff On 12/3/2012 12:00 AM, Daniela Bodemer wrote: > Hi all, > > > > Our tissue processor has been shut down due to a contamination issue and > now all the tissues (mice and rat pelves) collected prior to this > happening have been sitting in 4% PFA. Some tissues more than a week, > when we usually fix for 48 hours. > > Now we are transferring the tissues to 70% Ethanol and they will sit > there until further notice. I am concerned about this process and what > it will do to the tissue and would like your thoughts on this. > > > > Many thanks, > > > > DB > > Research Assistant > > > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From PAMarcum <@t> uams.edu Wed Dec 5 10:09:20 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Dec 5 10:10:25 2012 Subject: [Histonet] Histology Positions in Little Rock AR Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32924B5C23@Mail2Node2.ad.uams.edu> We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming. Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.) Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO) Best Regards, Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From TJohnson <@t> gnf.org Wed Dec 5 10:32:43 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Dec 5 10:32:47 2012 Subject: [Histonet] Re: Fixation time Message-ID: <9F3CFEE76E51B64991C7485270890B400CE128F3@EX4.lj.gnf.org> Hi Daniela, We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that could introduce variation in your experimental outcome. Be very critical of the staining results you obtain on these samples compared to your others. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From TJohnson <@t> gnf.org Wed Dec 5 10:37:56 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Dec 5 10:38:00 2012 Subject: [Histonet] Re: Tissue Processors Message-ID: <9F3CFEE76E51B64991C7485270890B400CE12902@EX4.lj.gnf.org> Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From AHutton <@t> dh.org Wed Dec 5 10:42:21 2012 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Dec 5 10:45:27 2012 Subject: [Histonet] job opening in PA In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE67A@dhmail.dhorg.org> Message-ID: <38A56C4F4630D348A50B3720409270870E0FE6A1@dhmail.dhorg.org> > Doylestown Hospital is Doylestown, PA has the following position open: > > Job Title: Histology Tech - 1123 > Req: 2012-2409 > Location: > Department: Laboratory > Schedule: Mon-Fri > varied start times 0530,0630,0730 > > Job Description: > PURPOSE OF JOB: To assist the Histology and cytology Department in the preparation of tissue and cells for microscope pathology. Performs Histologic and Cytologic procedures as states in the Histology and Cytology Procedure Manuals. ESSENTIAL FUNCTIONS: 45% 1. Properly orients, embeds and sections tissues at 5 microns without artifacts or contaminants. 25% 2. Performs routine, special, frozen and immunohistochemical staining. Manually prepares all stock and working solutions needed to perform above stains (Stain list is available in Histology). 20% 3. Accurately and legibly accessions specimens with the use of the computer. Assists pathologist with frozen sections. Follows slides through the H&E stainer and appropriately distributes slides to the pathologists. 5% 4. Operates and maintains all histology equipment, recycles chemicals and disposes of pathological waste, troubleshoots outliers to help keep department running efficiently. 5% 5. Covers the Cytology Department in the absence of the cytotechnologist. Assists Cytology and Office with designated duties. > For more information, please visit www.dh.org > > > From rjbuesa <@t> yahoo.com Wed Dec 5 10:54:40 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 5 10:54:48 2012 Subject: [Histonet] Re: Tissue Processors In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CE12902@EX4.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CE12902@EX4.lj.gnf.org> Message-ID: <1354726480.27777.YahooMailNeo@web163106.mail.bf1.yahoo.com> If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents?in a protocol using?isopropyl alcohol ? mineral oil ? paraffin?neither are noxious to the techs' health. This is my humble opinion. Ren? J. From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DEBORAH_ELLENBURG <@t> bshsi.org Wed Dec 5 11:13:51 2012 From: DEBORAH_ELLENBURG <@t> bshsi.org (Ellenburg, Deborah) Date: Wed Dec 5 11:14:02 2012 Subject: [Histonet] Re: Tissue Processors In-Reply-To: <1354726480.27777.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <9F3CFEE76E51B64991C7485270890B400CE12902@EX4.lj.gnf.org> <1354726480.27777.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: Well stated - agree 100%. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 05, 2012 11:55 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Tissue Processors If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents?in a protocol using?isopropyl alcohol ? mineral oil ? paraffin?neither are noxious to the techs' health. This is my humble opinion. Ren? J. From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From CDavis <@t> che-east.org Wed Dec 5 12:41:42 2012 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Wed Dec 5 12:41:58 2012 Subject: [Histonet] RE: processing protocol using isopropyl alcohol, mineral oil & paraffin Message-ID: <08861B9CF6C7774E874635A4818AE37B03791DF0@CHEXCMS01.one.ads.che.org> I'd really like to see a copy of the protocol if any one would be willing to share. We are still using reagent alcohol & xylene, the waste disposal prices are a significant portion of our budget. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From LPaveli1 <@t> hurleymc.com Wed Dec 5 12:55:35 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Dec 5 12:55:44 2012 Subject: [Histonet] RE: processing protocol using isopropyl alcohol, mineral oil & paraffin In-Reply-To: <08861B9CF6C7774E874635A4818AE37B03791DF0@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B03791DF0@CHEXCMS01.one.ads.che.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56C677@EXCHANGEMB1.hmc.hurleymc.com> Yes, please share! Thanks! Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Davis, Cassie [CDavis@che-east.org] Sent: Wednesday, December 05, 2012 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: processing protocol using isopropyl alcohol, mineral oil & paraffin I'd really like to see a copy of the protocol if any one would be willing to share. We are still using reagent alcohol & xylene, the waste disposal prices are a significant portion of our budget. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Dec 5 13:35:47 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Dec 5 13:35:49 2012 Subject: [Histonet] Leica Bond RX Message-ID: Good Day everyone, Is anyone using the new Leica Bond RX research IHC/ISH autostainer? If you are I would love to hear any feed back on it you may have. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net From MICHELLE.LAMPHERE <@t> childrens.com Wed Dec 5 14:34:50 2012 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Wed Dec 5 14:34:58 2012 Subject: [Histonet] Tissue Processors In-Reply-To: References: Message-ID: We currently have three Excelsiors and I would have a hard time going back to any other processor. I really appreciate the reagent management of this machine. We have virtually eliminated all hazardous reagent exposure during reagent exchange. I think their customer service is outstanding as well as their technical support and training. I have used the VIPs and the Leica ASP 300. The only downside that I have experienced with the Excelsior is that all three of our machines have a software glitch that will make the end date of a processing schedule get "stuck" on a previous date if the machine is run for a few days in a row and then taken off service for a few days. If you are not careful, a delayed start becomes an immediate start as the machine tries to backtrack to the previous date. Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, December 05, 2012 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: automated microtomes (Lynette Pavelich) 2. HistoTALK (David Kemler) 3. RE: Tissue Processors (Marcum, Pamela A) 4. RE: Tissue Processors (Boyd, Debbie M) 5. Re: Immunohistochemical detection of cytokines in older frozen sections (Rob Day) 6. RE: Histonet Digest, Vol 109, Issue 5 (Riesen, Rebecca) 7. Re: Fixation time (Geoff) 8. Histology Positions in Little Rock AR (Marcum, Pamela A) 9. Re: Fixation time (Teri Johnson) 10. Re: Tissue Processors (Teri Johnson) 11. job opening in PA (Hutton, Allison) 12. Re: Re: Tissue Processors (Rene J Buesa) 13. RE: Re: Tissue Processors (Ellenburg, Deborah) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Dec 2012 12:10:06 +0000 From: Lynette Pavelich Subject: RE: [Histonet] automated microtomes To: Rene J Buesa , "Rathborne, Toni" , "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF56C4F0@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="iso-8859-1" I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). I would go with the Leica. Ren? J. From: "Rathborne, Toni" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Dec 2012 04:28:54 -0800 (PST) From: David Kemler Subject: [Histonet] HistoTALK To: Fellow HistoNetters Message-ID: <1354710534.83756.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello HistoNetters - ? In case you missed our last two guests on HistoTALK http://www.histotalk.com/, they were Region VII Director, ANDI GRANTHAM and KATHERINE (Kaspar)?KASPRZYK, President of the Arizona Society for Histotechnology. Just wanted to let you know. ? Yours, David ------------------------------ Message: 3 Date: Wed, 5 Dec 2012 13:00:57 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Tissue Processors To: "'Susan.Walzer@HCAHealthcare.com'" , "rjbuesa@yahoo.com" , "twheelock@mclean.harvard.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA328E5D5138@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="iso-8859-1" We have 4 Thermo Excelsiors and prefer them to the VIP we no longer use. It is ease of changing reagents and programming as well as working better for our tissues. We also have Leica ASP300 that is used for only one type of tissue so we no longer need to change it weekly and expose personnel to the fumes of the reagents and possible spills. VIP are great units just not as easy to work with not that other options have become available. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, December 05, 2012 1:49 AM To: rjbuesa@yahoo.com; twheelock@mclean.harvard.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors VIP always! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2012 3:57 PM To: Tim Wheelock; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Processors I always used VIP because of reliability, "toughness" and customer service. Ren? J. From: Tim Wheelock To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 3:48 PM Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Wed, 5 Dec 2012 13:35:29 +0000 From: "Boyd, Debbie M" Subject: RE: [Histonet] Tissue Processors To: Tim Wheelock , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB762467949FE2A@TN001WEXMBX11.US.chs.net> Content-Type: text/plain; charset="us-ascii" Excelsior> Great workhorse. Very little to no down time. Less tech time needed as reagents dump and rotate automatically. User friendly. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Tuesday, December 04, 2012 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 5 Date: Wed, 5 Dec 2012 08:44:45 -0500 From: Rob Day Subject: Re: [Histonet] Immunohistochemical detection of cytokines in older frozen sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Folio biosciences is testing a process that will rejuvenate older sections to restore degraded antigenicity for ISH. It's designed to help scientists with exactly the problem you are describing Email me if you want to help us test this process using your slides. No charge of course. Rob Day. On Dec 4, 2012, at 6:26 PM, Darius Alex Schneider wrote: > Dear all, > > I am trying to detect cytokines in human pancreatic sections from > diabetics. The blocks are at least 1y old, the sections are usually fresh. > I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin, > staining overnight in BSS-saponin with the primary, biotinylated antibody > and subsequently tyramid signal amplification and fluorescein based > visuatlisation. However, my results are very poor. > I am mainly interested in these cytokines: > IL1beta > IL1r > IL2 > IL6 > IL10 > IL17 > IL21 > TNFalpha > IFNgamma > > Is there somebody out there who is experienced in detecting these > cytokines, and could provide me with a comprehensive protocol? Also, who > knows what the best positive controls might be? > > Thank you so much, > > Darius > La Jolla, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ------------------------------ Message: 6 Date: Wed, 5 Dec 2012 13:47:44 +0000 From: "Riesen, Rebecca" Subject: [Histonet] RE: Histonet Digest, Vol 109, Issue 5 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am in love with the Excelsior from the standpoint of hands off daily maintenance. Waste paraffin is pumped into a container with a liner, you let it solidify and remove waste in the bag for easy disposal. Pour a couple bags of paraffin pellets in and walk away. Take a bottle of waste alcohol or xylene the instrument has pumped into off and replace it with a new bottle. Excelsior will aspirate the new fluid on the next run. You don't even have to pour into another container. The Excelsior takes the fluids right out of the manufacturer's bottle. It is so simple! If you can read, that's all you need. The screen prompts everything. I also find my solutions last longer. ------------------------------ Message: 18 Date: Tue, 4 Dec 2012 15:39:11 -0800 From: Davide Costanzo Subject: Re: [Histonet] Tissue Processors To: Tim Wheelock Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1628083238193856494@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 The excelsior is getting rave reviews from folks I know that use it. The University of Miami just ordered a few of them from my understanding. Might want to call there and ask their opinion. Personally, I think Sakura is losing ground and for good reason. We use exclusively Thermo branded equipment here. STP420 and Pathcentre. Both are great. ------------------------------ Message: 7 Date: Wed, 05 Dec 2012 09:51:53 -0500 From: Geoff Subject: Re: [Histonet] Fixation time To: histonet@lists.utsouthwestern.edu Message-ID: <50BF5F89.20200@umdnj.edu> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed I agree with Rene. Geoff On 12/3/2012 12:00 AM, Daniela Bodemer wrote: > Hi all, > > > > Our tissue processor has been shut down due to a contamination issue and > now all the tissues (mice and rat pelves) collected prior to this > happening have been sitting in 4% PFA. Some tissues more than a week, > when we usually fix for 48 hours. > > Now we are transferring the tissues to 70% Ethanol and they will sit > there until further notice. I am concerned about this process and what > it will do to the tissue and would like your thoughts on this. > > > > Many thanks, > > > > DB > > Research Assistant > > > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 8 Date: Wed, 5 Dec 2012 16:09:20 +0000 From: "Marcum, Pamela A" Subject: [Histonet] Histology Positions in Little Rock AR To: "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32924B5C23@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming. Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.) Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO) Best Regards, Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Wed, 5 Dec 2012 16:32:43 +0000 From: Teri Johnson Subject: [Histonet] Re: Fixation time To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B400CE128F3@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Hi Daniela, We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that could introduce variation in your experimental outcome. Be very critical of the staining results you obtain on these samples compared to your others. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 10 Date: Wed, 5 Dec 2012 16:37:56 +0000 From: Teri Johnson Subject: [Histonet] Re: Tissue Processors To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B400CE12902@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 11 Date: Wed, 5 Dec 2012 11:42:21 -0500 From: "Hutton, Allison" Subject: [Histonet] job opening in PA To: Message-ID: <38A56C4F4630D348A50B3720409270870E0FE6A1@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" > Doylestown Hospital is Doylestown, PA has the following position open: > > Job Title: Histology Tech - 1123 > Req: 2012-2409 > Location: > Department: Laboratory > Schedule: Mon-Fri > varied start times 0530,0630,0730 > > Job Description: > PURPOSE OF JOB: To assist the Histology and cytology Department in the preparation of tissue and cells for microscope pathology. Performs Histologic and Cytologic procedures as states in the Histology and Cytology Procedure Manuals. ESSENTIAL FUNCTIONS: 45% 1. Properly orients, embeds and sections tissues at 5 microns without artifacts or contaminants. 25% 2. Performs routine, special, frozen and immunohistochemical staining. Manually prepares all stock and working solutions needed to perform above stains (Stain list is available in Histology). 20% 3. Accurately and legibly accessions specimens with the use of the computer. Assists pathologist with frozen sections. Follows slides through the H&E stainer and appropriately distributes slides to the pathologists. 5% 4. Operates and maintains all histology equipment, recycles chemicals and disposes of pathological waste, troubleshoots outliers to help keep department running efficiently. 5% 5. Covers the Cytology Department in the absence of the cytotechnologist. Assists Cytology and Office with designated duties. > For more information, please visit www.dh.org > > > ------------------------------ Message: 12 Date: Wed, 5 Dec 2012 08:54:40 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Re: Tissue Processors To: Teri Johnson , "histonet@lists.utsouthwestern.edu" Message-ID: <1354726480.27777.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health. This is my humble opinion. Ren?? J. From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 5 Dec 2012 12:13:51 -0500 From: "Ellenburg, Deborah" Subject: RE: [Histonet] Re: Tissue Processors To: "Rene J Buesa" , "Teri Johnson" , Message-ID: Content-Type: text/plain; charset="UTF-8" Well stated - agree 100%. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 05, 2012 11:55 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Tissue Processors If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health. This is my humble opinion. Ren?? J. From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. 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Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 109, Issue 6 **************************************** Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. 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From mbmphoto <@t> gmail.com Wed Dec 5 15:03:25 2012 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Wed Dec 5 15:03:38 2012 Subject: [Histonet] processing protocol using isopropyl alcohol, mineral oil & paraffin In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56C677@EXCHANGEMB1.hmc.hurleymc.com> References: <08861B9CF6C7774E874635A4818AE37B03791DF0@CHEXCMS01.one.ads.che.org> <89F4666A496DC949A819ECC40E11C867BF56C677@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <6F39D59E-E6B7-44E5-83C0-A0247B2F4F7C@gmail.com> I'd like to be included in the protocol processing exchange too! Best Maria Mejia On Dec 5, 2012, at 10:55 AM, Lynette Pavelich wrote: > Yes, please share! Thanks! > > Lynette > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Davis, Cassie [CDavis@che-east.org] > Sent: Wednesday, December 05, 2012 1:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: processing protocol using isopropyl alcohol, mineral oil & paraffin > > I'd really like to see a copy of the protocol if any one would be willing to share. We are still using reagent alcohol & xylene, the waste disposal prices are a significant portion of our budget. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Wed Dec 5 17:52:53 2012 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Dec 5 17:53:00 2012 Subject: [Histonet] Trichrome In-Reply-To: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> References: <1354627115.8370.YahooMailClassic@web39405.mail.mud.yahoo.com> Message-ID: <1354751573.77799.YahooMailNeo@web172003.mail.ir2.yahoo.com> Hi, In Trichrome staining not excellent results are always obtained by fixing in NBF alone. Often the advised fixatives are: - Sublimate-acetic - Zenker's liquid - Telleyesnicky' s liquid - M?ller's liquid added with a formalin solution - or re-fixing ?by Bouin's liquid I'm talking about staining by hand. Best Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Cheryl A: histonet@lists.utsouthwestern.edu Inviato: Marted? 4 Dicembre 2012 14:18 Oggetto: [Histonet] Trichrome Hi- need help troubleshooting a trichrome.? The stain on the Nexus stainers is inconsistent.? Taking it off the stainer and doing it by hand with a kit has similar results. ? The tissue is NBF fixed needle biopsies on kidney.? There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.? The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... ? Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Thu Dec 6 01:52:59 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Dec 6 01:53:06 2012 Subject: [Histonet] Re: Histonet Digest, Vol 109, Issue 6 In-Reply-To: <50bf8bf0.65b4ec0a.5a89.3ed5SMTPIN_ADDED_MISSING@mx.google.com> References: <50bf8bf0.65b4ec0a.5a89.3ed5SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Tuesday, December 04, 2012 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors Hello Tim, Here are some of the advantages I found with Leica-Peloris "rapid tissue processor" (I can not comment for below 3). 1. Small biopsies take only 1 hours (VIP5 take at least 2 - 4 hrs) 2. Regular surgical tissues take only 2 - 4 hours 3. Fatty breast tissues are very well processor with only 8 hours (VIP5 take overnight or at least 12-16 hrs) Most importantly, the integrity of these late grossing and fatty tissues are far more superior than the VIP5. Another advantage is cost effective. We use only fraction of the reagents (Xylene, alcohol & paraffin) as compare to the VIP. Here's my suggestion before you make any final decisions; - gross multiple types of un-used tissues from your lab with multiple blocks from same tissues - send one cassette from each tissue type to the vendors for processing (make sure get protocols from them) - embed, cut & HE in your lab Let the pathologist rate the slides. Happy hunting! Madeleine Huey BS, HTL & QIHC (ASCP) Supervisor - Pathology (Histology & IPOX) madeleine_h@elcaminohospital Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA From LStadler <@t> cbiolabs.com Thu Dec 6 09:31:03 2012 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Thu Dec 6 09:31:26 2012 Subject: [Histonet] Processing Protocol Using Mineral Oil Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC010E035EE1@cbiolabs05.CBiolabs.local> We have been using this protocol on mostly mouse tissues with great results! We run this on a VIP 3000. 70% Ethanol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 5:1 Iso:Mineral Oil, 50 degrees, 1 hour 2:1 Iso:Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 6 hours Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Followed by a cleaning cycle after cassettes are removed and put into molten paraffin at the embedding center Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, December 05, 2012 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: automated microtomes (Lynette Pavelich) 2. HistoTALK (David Kemler) 3. RE: Tissue Processors (Marcum, Pamela A) 4. RE: Tissue Processors (Boyd, Debbie M) 5. Re: Immunohistochemical detection of cytokines in older frozen sections (Rob Day) 6. RE: Histonet Digest, Vol 109, Issue 5 (Riesen, Rebecca) 7. Re: Fixation time (Geoff) 8. Histology Positions in Little Rock AR (Marcum, Pamela A) 9. Re: Fixation time (Teri Johnson) 10. Re: Tissue Processors (Teri Johnson) 11. job opening in PA (Hutton, Allison) 12. Re: Re: Tissue Processors (Rene J Buesa) 13. RE: Re: Tissue Processors (Ellenburg, Deborah) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Dec 2012 12:10:06 +0000 From: Lynette Pavelich Subject: RE: [Histonet] automated microtomes To: Rene J Buesa , "Rathborne, Toni" , "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF56C4F0@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="iso-8859-1" I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). I would go with the Leica. Ren? J. From: "Rathborne, Toni" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Dec 2012 04:28:54 -0800 (PST) From: David Kemler Subject: [Histonet] HistoTALK To: Fellow HistoNetters Message-ID: <1354710534.83756.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello HistoNetters - ? In case you missed our last two guests on HistoTALK http://www.histotalk.com/, they were Region VII Director, ANDI GRANTHAM and KATHERINE (Kaspar)?KASPRZYK, President of the Arizona Society for Histotechnology. Just wanted to let you know. ? Yours, David ------------------------------ Message: 3 Date: Wed, 5 Dec 2012 13:00:57 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Tissue Processors To: "'Susan.Walzer@HCAHealthcare.com'" , "rjbuesa@yahoo.com" , "twheelock@mclean.harvard.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA328E5D5138@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="iso-8859-1" We have 4 Thermo Excelsiors and prefer them to the VIP we no longer use. It is ease of changing reagents and programming as well as working better for our tissues. We also have Leica ASP300 that is used for only one type of tissue so we no longer need to change it weekly and expose personnel to the fumes of the reagents and possible spills. VIP are great units just not as easy to work with not that other options have become available. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, December 05, 2012 1:49 AM To: rjbuesa@yahoo.com; twheelock@mclean.harvard.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors VIP always! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2012 3:57 PM To: Tim Wheelock; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Processors I always used VIP because of reliability, "toughness" and customer service. Ren? J. From: Tim Wheelock To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 3:48 PM Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Wed, 5 Dec 2012 13:35:29 +0000 From: "Boyd, Debbie M" Subject: RE: [Histonet] Tissue Processors To: Tim Wheelock , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB762467949FE2A@TN001WEXMBX11.US.chs.net> Content-Type: text/plain; charset="us-ascii" Excelsior> Great workhorse. Very little to no down time. Less tech time needed as reagents dump and rotate automatically. User friendly. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Tuesday, December 04, 2012 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 5 Date: Wed, 5 Dec 2012 08:44:45 -0500 From: Rob Day Subject: Re: [Histonet] Immunohistochemical detection of cytokines in older frozen sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Folio biosciences is testing a process that will rejuvenate older sections to restore degraded antigenicity for ISH. It's designed to help scientists with exactly the problem you are describing Email me if you want to help us test this process using your slides. No charge of course. Rob Day. On Dec 4, 2012, at 6:26 PM, Darius Alex Schneider wrote: > Dear all, > > I am trying to detect cytokines in human pancreatic sections from > diabetics. The blocks are at least 1y old, the sections are usually fresh. > I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin, > staining overnight in BSS-saponin with the primary, biotinylated antibody > and subsequently tyramid signal amplification and fluorescein based > visuatlisation. However, my results are very poor. > I am mainly interested in these cytokines: > IL1beta > IL1r > IL2 > IL6 > IL10 > IL17 > IL21 > TNFalpha > IFNgamma > > Is there somebody out there who is experienced in detecting these > cytokines, and could provide me with a comprehensive protocol? Also, who > knows what the best positive controls might be? > > Thank you so much, > > Darius > La Jolla, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ------------------------------ Message: 6 Date: Wed, 5 Dec 2012 13:47:44 +0000 From: "Riesen, Rebecca" Subject: [Histonet] RE: Histonet Digest, Vol 109, Issue 5 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am in love with the Excelsior from the standpoint of hands off daily maintenance. Waste paraffin is pumped into a container with a liner, you let it solidify and remove waste in the bag for easy disposal. Pour a couple bags of paraffin pellets in and walk away. Take a bottle of waste alcohol or xylene the instrument has pumped into off and replace it with a new bottle. Excelsior will aspirate the new fluid on the next run. You don't even have to pour into another container. The Excelsior takes the fluids right out of the manufacturer's bottle. It is so simple! If you can read, that's all you need. The screen prompts everything. I also find my solutions last longer. ------------------------------ Message: 18 Date: Tue, 4 Dec 2012 15:39:11 -0800 From: Davide Costanzo Subject: Re: [Histonet] Tissue Processors To: Tim Wheelock Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1628083238193856494@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 The excelsior is getting rave reviews from folks I know that use it. The University of Miami just ordered a few of them from my understanding. Might want to call there and ask their opinion. Personally, I think Sakura is losing ground and for good reason. We use exclusively Thermo branded equipment here. STP420 and Pathcentre. Both are great. ------------------------------ Message: 7 Date: Wed, 05 Dec 2012 09:51:53 -0500 From: Geoff Subject: Re: [Histonet] Fixation time To: histonet@lists.utsouthwestern.edu Message-ID: <50BF5F89.20200@umdnj.edu> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed I agree with Rene. Geoff On 12/3/2012 12:00 AM, Daniela Bodemer wrote: > Hi all, > > > > Our tissue processor has been shut down due to a contamination issue and > now all the tissues (mice and rat pelves) collected prior to this > happening have been sitting in 4% PFA. Some tissues more than a week, > when we usually fix for 48 hours. > > Now we are transferring the tissues to 70% Ethanol and they will sit > there until further notice. I am concerned about this process and what > it will do to the tissue and would like your thoughts on this. > > > > Many thanks, > > > > DB > > Research Assistant > > > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 8 Date: Wed, 5 Dec 2012 16:09:20 +0000 From: "Marcum, Pamela A" Subject: [Histonet] Histology Positions in Little Rock AR To: "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32924B5C23@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming. Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.) Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO) Best Regards, Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Wed, 5 Dec 2012 16:32:43 +0000 From: Teri Johnson Subject: [Histonet] Re: Fixation time To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B400CE128F3@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Hi Daniela, We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that could introduce variation in your experimental outcome. Be very critical of the staining results you obtain on these samples compared to your others. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 10 Date: Wed, 5 Dec 2012 16:37:56 +0000 From: Teri Johnson Subject: [Histonet] Re: Tissue Processors To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B400CE12902@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 11 Date: Wed, 5 Dec 2012 11:42:21 -0500 From: "Hutton, Allison" Subject: [Histonet] job opening in PA To: Message-ID: <38A56C4F4630D348A50B3720409270870E0FE6A1@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" > Doylestown Hospital is Doylestown, PA has the following position open: > > Job Title: Histology Tech - 1123 > Req: 2012-2409 > Location: > Department: Laboratory > Schedule: Mon-Fri > varied start times 0530,0630,0730 > > Job Description: > PURPOSE OF JOB: To assist the Histology and cytology Department in the preparation of tissue and cells for microscope pathology. Performs Histologic and Cytologic procedures as states in the Histology and Cytology Procedure Manuals. ESSENTIAL FUNCTIONS: 45% 1. Properly orients, embeds and sections tissues at 5 microns without artifacts or contaminants. 25% 2. Performs routine, special, frozen and immunohistochemical staining. Manually prepares all stock and working solutions needed to perform above stains (Stain list is available in Histology). 20% 3. Accurately and legibly accessions specimens with the use of the computer. Assists pathologist with frozen sections. Follows slides through the H&E stainer and appropriately distributes slides to the pathologists. 5% 4. Operates and maintains all histology equipment, recycles chemicals and disposes of pathological waste, troubleshoots outliers to help keep department running efficiently. 5% 5. Covers the Cytology Department in the absence of the cytotechnologist. Assists Cytology and Office with designated duties. > For more information, please visit www.dh.org > > > ------------------------------ Message: 12 Date: Wed, 5 Dec 2012 08:54:40 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Re: Tissue Processors To: Teri Johnson , "histonet@lists.utsouthwestern.edu" Message-ID: <1354726480.27777.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health. This is my humble opinion. Ren?? J. From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 5 Dec 2012 12:13:51 -0500 From: "Ellenburg, Deborah" Subject: RE: [Histonet] Re: Tissue Processors To: "Rene J Buesa" , "Teri Johnson" , Message-ID: Content-Type: text/plain; charset="UTF-8" Well stated - agree 100%. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 05, 2012 11:55 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Tissue Processors If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health. This is my humble opinion. Ren?? J. From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 109, Issue 6 **************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From joewalker <@t> rrmc.org Thu Dec 6 10:45:10 2012 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Thu Dec 6 10:45:20 2012 Subject: [Histonet] Disposal of DAB-link to Princeton. In-Reply-To: References: <006301cdcda9$2eddd730$8c998590$@com>, Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC179828B8@RRMBX03.rrmc.local> Please correct me if I am wrong but if you use potassium permanganate sulfuric acid, do you not deactivate the mutagenic properties? Then disposal would not require a hazardous waste container. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, December 03, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of DAB-link to Princeton. DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry > From: chapcl@yahoo.com > Date: Wed, 28 Nov 2012 13:14:26 -0800 > To: cpyse@x-celllab.com > Subject: Re: [Histonet] chemical disposal > CC: histonet@lists.utsouthwestern.edu > > Chemical hazardous waste. > > Sent from my iPhone > > On Nov 28, 2012, at 12:44 PM, "Cynthia Pyse" wrote: > > > Quick question for Histoland. I am having a debate about DAB > > disposal. Our general manager ( non lab background) insists that the > > liquid DAB can go into a biological hazardous waste. I disagree, it > > is a chemical and needs to be disposed in the chemical hazardous > > waste. What is everyone else doing to dispose of DAB. We are located > > in NY, I do have those regs. Thanks in advance for any and all help. > > > > Cindy > > > > > > > > Cindy Pyse, CLT, HT (ASCP) > > > > Laboratory Manager > > > > X-Cell Laboratories > > > > 20 Northpointe Parkway Suite 100 > > > > Amherst, NY 14228 > > > > 716-250-9235 etx. 232 > > > > e-mail cpyse@x-celllab.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From HornHV <@t> archildrens.org Thu Dec 6 10:51:00 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Dec 6 10:51:09 2012 Subject: [Histonet] Disposal of DAB-link to Princeton. In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC179828B8@RRMBX03.rrmc.local> References: <006301cdcda9$2eddd730$8c998590$@com>, <3C2378778400AD448ADA6FD6BDB7CCCC179828B8@RRMBX03.rrmc.local> Message-ID: <25A4DE08332B19499904459F00AAACB719BE135494@EVS1.archildrens.org> That's exactly what we do. After the potassium permanganate /sulfuric acid we neutralize it and put in down the drain with copious amounts of water. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Thursday, December 06, 2012 10:45 AM To: Jerry Ricks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of DAB-link to Princeton. Please correct me if I am wrong but if you use potassium permanganate sulfuric acid, do you not deactivate the mutagenic properties? Then disposal would not require a hazardous waste container. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, December 03, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of DAB-link to Princeton. DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry > From: chapcl@yahoo.com > Date: Wed, 28 Nov 2012 13:14:26 -0800 > To: cpyse@x-celllab.com > Subject: Re: [Histonet] chemical disposal > CC: histonet@lists.utsouthwestern.edu > > Chemical hazardous waste. > > Sent from my iPhone > > On Nov 28, 2012, at 12:44 PM, "Cynthia Pyse" wrote: > > > Quick question for Histoland. I am having a debate about DAB > > disposal. Our general manager ( non lab background) insists that the > > liquid DAB can go into a biological hazardous waste. I disagree, it > > is a chemical and needs to be disposed in the chemical hazardous > > waste. What is everyone else doing to dispose of DAB. We are located > > in NY, I do have those regs. Thanks in advance for any and all help. > > > > Cindy > > > > > > > > Cindy Pyse, CLT, HT (ASCP) > > > > Laboratory Manager > > > > X-Cell Laboratories > > > > 20 Northpointe Parkway Suite 100 > > > > Amherst, NY 14228 > > > > 716-250-9235 etx. 232 > > > > e-mail cpyse@x-celllab.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 6 11:54:42 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 6 11:56:41 2012 Subject: [Histonet] Disposal of DAB-link to Princeton. In-Reply-To: <25A4DE08332B19499904459F00AAACB719BE135494@EVS1.archildrens.org> References: <006301cdcda9$2eddd730$8c998590$@com>, <3C2378778400AD448ADA6FD6BDB7CCCC179828B8@RRMBX03.rrmc.local> <25A4DE08332B19499904459F00AAACB719BE135494@EVS1.archildrens.org> Message-ID: <761E2B5697F795489C8710BCC72141FF02E9AB@ex07.net.ucsf.edu> Everyone in histology should get a copy of Anatech's book "Hazardous Waste In the Histopathology Laboratory." It goes through all this in detail. http://www.anatechltdusa.com/Hazmat.html Catalog # 051 $88.40 Hazmat Manual New Features Hardbound Fully indexed Convenient 6" x 9" size Comprehensive revisions throughout, including... updated references and resources extensive list of contacts with web addresses latest recommendations from standard-setting organizations new chapter on ASCP/BOR Specialist in Laboratory Safety certification exam -------------------------------------------------------------------------------- Order your copy now! Call 1.800.262.8324 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, December 06, 2012 8:51 AM To: 'Joe W. Walker, Jr.'; Jerry Ricks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of DAB-link to Princeton. That's exactly what we do. After the potassium permanganate /sulfuric acid we neutralize it and put in down the drain with copious amounts of water. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Thursday, December 06, 2012 10:45 AM To: Jerry Ricks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of DAB-link to Princeton. Please correct me if I am wrong but if you use potassium permanganate sulfuric acid, do you not deactivate the mutagenic properties? Then disposal would not require a hazardous waste container. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, December 03, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of DAB-link to Princeton. DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry > From: chapcl@yahoo.com > Date: Wed, 28 Nov 2012 13:14:26 -0800 > To: cpyse@x-celllab.com > Subject: Re: [Histonet] chemical disposal > CC: histonet@lists.utsouthwestern.edu > > Chemical hazardous waste. > > Sent from my iPhone > > On Nov 28, 2012, at 12:44 PM, "Cynthia Pyse" wrote: > > > Quick question for Histoland. I am having a debate about DAB > > disposal. Our general manager ( non lab background) insists that the > > liquid DAB can go into a biological hazardous waste. I disagree, it > > is a chemical and needs to be disposed in the chemical hazardous > > waste. What is everyone else doing to dispose of DAB. We are located > > in NY, I do have those regs. Thanks in advance for any and all help. > > > > Cindy > > > > > > > > Cindy Pyse, CLT, HT (ASCP) > > > > Laboratory Manager > > > > X-Cell Laboratories > > > > 20 Northpointe Parkway Suite 100 > > > > Amherst, NY 14228 > > > > 716-250-9235 etx. 232 > > > > e-mail cpyse@x-celllab.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkrupp <@t> deltacollege.edu Thu Dec 6 12:10:31 2012 From: jkrupp <@t> deltacollege.edu (Jon Krupp) Date: Thu Dec 6 12:10:36 2012 Subject: [Histonet] How to open Reichert 820? Message-ID: <09FFDB2C-3CAD-4342-B6BB-4A5299D72BC8@deltacollege.edu> Hi Anyone know how to open the cover of a Reichert 820 microtome? This is the model that has a wheel for the coarse advance on the left side. I am used to the AO Style, pop the latch & tip it back. These usually have a crank and a cut out slot on the left. This one uses 4 screws from the bottom to secure the lid and there is no cut out for the coarse advance to slide through. Looks like the wheel has to be removed to remove the cover. Getting the wheel off is where I am stuck. This is an old microtome we have had sitting around, would like to check the guts and clean it up. Jon Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College From jclark <@t> pcnm.com Thu Dec 6 12:32:49 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Dec 6 12:33:01 2012 Subject: [Histonet] DAKO Her2 In-Reply-To: <20121206174102.3374463E878@mx10.myoutlookonline.com> References: <20121206174102.3374463E878@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E37894637A37@S10MAILD001N4.SH10.lan> Hi All, sorry for how I am submitting this but I have been sending in questions to the address provided by nothing is going through. I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. We want to start running this on our Leica BOND and any info would be much appreciated. Thanks Joanne Clark, HT(ASCP) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM From twheelock <@t> mclean.harvard.edu Thu Dec 6 14:28:50 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Thu Dec 6 14:28:54 2012 Subject: [Histonet] Embedding Centers Message-ID: <50C10002.8070708@mclean.harvard.edu> Hi Everyone: I am also in the market for a new paraffin embedding center. I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the Thermo-Fisher HistoStar. I was wondering if people could give me their critical opinion on these, or other machines. What sorts of problems have you had with them. I currently have a 25 year old Shandon Embedding Center. I like it a lot. But I would like to find a machine with a specimen holding tank large enough to allow me to immerse 300 cassettes all at once. This is because I infiltrate brain tissue with Tissue Path Paraplast but embed with Surgipath Embedding Media So I let the cassettes sit immersed in the Surgipath for an hour or two before embedding. (Until I can buy a new processor, The Shandon's holding tank also serves as a third processing station, since my Shandon Hypercenter has only 2 wax reservoirs) I also do not feel comfortable having the cassettes sitting dry in the holding tank Thanks, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA From billodonnell <@t> catholichealth.net Thu Dec 6 14:41:28 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Dec 6 14:41:12 2012 Subject: [Histonet] Embedding Centers In-Reply-To: <50C10002.8070708@mclean.harvard.edu> References: <50C10002.8070708@mclean.harvard.edu> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93958EF23C@chimsx08.CHI.catholichealth.net> Tim - Tissue-Tek 5 has been trouble-free for the 4 years we have had it with one exception. Early on something went goofy with the dispensing mechanism. Sakura fixed it promptly and it has been great ever since. - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Thursday, December 06, 2012 2:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Centers Hi Everyone: I am also in the market for a new paraffin embedding center. I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the Thermo-Fisher HistoStar. I was wondering if people could give me their critical opinion on these, or other machines. What sorts of problems have you had with them. I currently have a 25 year old Shandon Embedding Center. I like it a lot. But I would like to find a machine with a specimen holding tank large enough to allow me to immerse 300 cassettes all at once. This is because I infiltrate brain tissue with Tissue Path Paraplast but embed with Surgipath Embedding Media So I let the cassettes sit immersed in the Surgipath for an hour or two before embedding. (Until I can buy a new processor, The Shandon's holding tank also serves as a third processing station, since my Shandon Hypercenter has only 2 wax reservoirs) I also do not feel comfortable having the cassettes sitting dry in the holding tank Thanks, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From andrewcoleman131 <@t> gmail.com Thu Dec 6 14:43:09 2012 From: andrewcoleman131 <@t> gmail.com (Andrew Coleman) Date: Thu Dec 6 14:43:14 2012 Subject: [Histonet] Paraformaldehyde Solution Recipe for Perfusion Message-ID: Hi all, We are performing transcardial perfusions in rats using paraformaldehyde in 0.1M potassium phosphate buffer. Can anyone think of any issues that would be caused by using phosphate buffer made from solely potassium salts (basic and dibasic), rather than a mixture of sodium and potassium or only the sodium salts? We do our rinse with 0.1 M PB + Saline and then follow up with solutions just made up in the potassium phosphate buffer (therefore no Na+). Could this cause any tonicity/osmolarity issues? We are trying to troubleshoot some issues we are having with the perfused tissue. Thanks! - Andrew From jaylundgren <@t> gmail.com Thu Dec 6 15:21:54 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Dec 6 15:21:59 2012 Subject: [Histonet] automated microtomes In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56C4F0@EXCHANGEMB1.hmc.hurleymc.com> References: <3AD061FE740D464FAC7BF6B5CFB757071F1F15BD@smcmail02.somerset-healthcare.com> <1354645189.12776.YahooMailNeo@web163102.mail.bf1.yahoo.com> <89F4666A496DC949A819ECC40E11C867BF56C4F0@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: Leica is making the sweetest 'tomes out there at the moment, IMHO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich wrote: > I am purchasing my second Leica RM2255 automated microtome. Rene' is > correct in saying that the carpal tunnel syndrome will eventually affect > every advanced tech by using any microtome manually. On the RM2255, the > flywheel additionally is automated. You can choose to use the flywheel > manually or automated by the simultaneous pushing of two buttons. Nice > feature for us "oldies" to ease us into automation or if you have a tiny > specimen that you need to take extra care with and want to use manually. > Companies are very happy to send in a demo to try for a couple weeks. > > Happy shopping!! ;) > Lynette > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [ > rjbuesa@yahoo.com] > Sent: Tuesday, December 04, 2012 1:19 PM > To: Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] automated microtomes > > The advantage of the so called automated microtomes (the only thing > automated about them is the block advance) is that they alleviate wrist > effort and in some ways prevent carpal tunnel syndrome that affects some > histotechs (mostly of the "senior persuasion"). > I would go with the Leica. > Ren? J. > > From: "Rathborne, Toni" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, December 4, 2012 11:48 AM > Subject: [Histonet] automated microtomes > > I'm looking for some opinions about the automated microtomes currently > available. Which ones do most techs prefer? Which are more reliable? Is > there an advantage to having a semi-automated microtome? > Thanks in advance for your replies. > > Toni > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lynn.Burton <@t> Illinois.gov Thu Dec 6 15:21:58 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Dec 6 15:22:07 2012 Subject: [Histonet] Embedding Centers In-Reply-To: <50C10002.8070708@mclean.harvard.edu> References: <50C10002.8070708@mclean.harvard.edu> Message-ID: Both animal disease labs in the state of Illinois have used the Sakura machines for the twenty years I have been here with great success. We also have aSakura processor that has been going for 25 years and a coverslipper that has only had 3 service calls for minor problems in the past 15+ years. They make good products. Lynn Burton Animal Disease Lab Galesburg, Il -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Thursday, December 06, 2012 2:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Centers Hi Everyone: I am also in the market for a new paraffin embedding center. I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the Thermo-Fisher HistoStar. I was wondering if people could give me their critical opinion on these, or other machines. What sorts of problems have you had with them. I currently have a 25 year old Shandon Embedding Center. I like it a lot. But I would like to find a machine with a specimen holding tank large enough to allow me to immerse 300 cassettes all at once. This is because I infiltrate brain tissue with Tissue Path Paraplast but embed with Surgipath Embedding Media So I let the cassettes sit immersed in the Surgipath for an hour or two before embedding. (Until I can buy a new processor, The Shandon's holding tank also serves as a third processing station, since my Shandon Hypercenter has only 2 wax reservoirs) I also do not feel comfortable having the cassettes sitting dry in the holding tank Thanks, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Dec 6 15:47:18 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Dec 6 15:47:54 2012 Subject: [Histonet] automated microtomes In-Reply-To: References: <3AD061FE740D464FAC7BF6B5CFB757071F1F15BD@smcmail02.somerset-healthcare.com> <1354645189.12776.YahooMailNeo@web163102.mail.bf1.yahoo.com> <89F4666A496DC949A819ECC40E11C867BF56C4F0@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757071F1F6772@smcmail02.somerset-healthcare.com> Thanks all for your valued opinions. I have contacted Leica, and am now waiting for a demo! From: Jay Lundgren [mailto:jaylundgren@gmail.com] Sent: Thursday, December 06, 2012 4:22 PM To: Lynette Pavelich Cc: Rene J Buesa; Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes Leica is making the sweetest 'tomes out there at the moment, IMHO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich > wrote: I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). I would go with the Leica. Ren? J. From: "Rathborne, Toni" > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Dec 6 16:40:47 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Dec 6 16:40:51 2012 Subject: [Histonet] refurbished histology equipment Message-ID: <311FA59E257C47B99E7C538F1DB384AE@DESKTOP3> Does anyone have a favorite vendor they would recommend to me? I am mostly looking for cryostats right now, but interested in other things, tissue processors, IHC stainers, etc. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net From sadey <@t> hotmail.ca Thu Dec 6 19:58:10 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Thu Dec 6 19:58:16 2012 Subject: [Histonet] Alcian Blue Message-ID: Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila From Susan.Walzer <@t> HCAHealthcare.com Fri Dec 7 02:04:37 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Dec 7 02:04:48 2012 Subject: [Histonet] Alcian Blue In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DF8521C13@FWDCWPMSGCMS09.hca.corpad.net> We've always used 30 minutes and it has never failed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Fri Dec 7 06:06:15 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Dec 7 06:06:30 2012 Subject: [Histonet] Alcian Blue In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DF8521C13@FWDCWPMSGCMS09.hca.corpad.net> References: , <4BF03F5404EBDE409AF9232DA74B9DED2DF8521C13@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56C8FB@EXCHANGEMB1.hmc.hurleymc.com> 30 here..... Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Susan.Walzer@HCAHealthcare.com [Susan.Walzer@HCAHealthcare.com] Sent: Friday, December 07, 2012 3:04 AM To: sadey@hotmail.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Alcian Blue We've always used 30 minutes and it has never failed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Dec 7 08:11:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 7 08:11:55 2012 Subject: [Histonet] Alcian Blue In-Reply-To: References: Message-ID: <1354889510.81416.YahooMailNeo@web163105.mail.bf1.yahoo.com> If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them "in house" check the preparation date and prepare a fresh solution. The general procedure calls for 30 min, unless you use a microwave oven version of the staining protocol. Ren? J. From: Sheila Adey To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, December 6, 2012 8:58 PM Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Dec 7 09:50:09 2012 From: mcauliff <@t> umdnj.edu (Geoff) Date: Fri Dec 7 09:50:15 2012 Subject: [Histonet] Paraformaldehyde Solution Recipe for Perfusion In-Reply-To: References: Message-ID: <50C21031.7060405@umdnj.edu> What issues are you having? That might help the list diagnose the problem. Geoff On 12/6/2012 3:43 PM, Andrew Coleman wrote: > Hi all, > > We are performing transcardial perfusions in rats using paraformaldehyde in > 0.1M potassium phosphate buffer. > > Can anyone think of any issues that would be caused by using phosphate > buffer made from solely potassium salts (basic and dibasic), rather than a > mixture of sodium and potassium or only the sodium salts? We do our rinse > with 0.1 M PB + Saline and then follow up with solutions just made up in > the potassium phosphate buffer (therefore no Na+). > > Could this cause any tonicity/osmolarity issues? We are trying to > troubleshoot some issues we are having with the perfused tissue. > > Thanks! - Andrew > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From twheelock <@t> mclean.harvard.edu Fri Dec 7 11:44:11 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Dec 7 11:44:17 2012 Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION Message-ID: <50C22AEB.6070508@mclean.harvard.edu> Hi All: First, thank you for all your feedback on the processors. I site-visited a VIP6 at a local hospital From my understanding the VIP6 can rotate absolute alcohol and xylene (using the bulk reservoirs), as well as the paraffin stations, but it cannot rotate other concentrations of alcohol based on hydrometer readings. Am I correct in this appraisal? Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA From m.kap.1 <@t> erasmusmc.nl Fri Dec 7 12:09:19 2012 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Fri Dec 7 12:09:28 2012 Subject: [Histonet] Message 2: dako her2 In-Reply-To: References: Message-ID: <0F8D6142-0252-4E60-A8BD-207E0511D00A@erasmusmc.nl> Check www.cqpath.com for more info Verstuurd vanaf mijn iPad Op 7 dec. 2012 om 19:03 heeft "histonet-request@lists.utsouthwestern.edu" het volgende geschreven: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. How to open Reichert 820? (Jon Krupp) > 2. DAKO Her2 (Joanne Clark) > 3. Embedding Centers (Tim Wheelock) > 4. RE: Embedding Centers (O'Donnell, Bill) > 5. Paraformaldehyde Solution Recipe for Perfusion (Andrew Coleman) > 6. Re: automated microtomes (Jay Lundgren) > 7. RE: Embedding Centers (Burton, Lynn) > 8. RE: automated microtomes (Rathborne, Toni) > 9. refurbished histology equipment (Patsy Ruegg) > 10. Alcian Blue (Sheila Adey) > 11. RE: Alcian Blue (Susan.Walzer@HCAHealthcare.com) > 12. RE: Alcian Blue (Lynette Pavelich) > 13. Re: Alcian Blue (Rene J Buesa) > 14. Re: Paraformaldehyde Solution Recipe for Perfusion (Geoff) > 15. TISSUE PROCESSOR FOLLOW-UP QUESTION (Tim Wheelock) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 6 Dec 2012 10:10:31 -0800 > From: Jon Krupp > Subject: [Histonet] How to open Reichert 820? > To: HISTONET > Message-ID: <09FFDB2C-3CAD-4342-B6BB-4A5299D72BC8@deltacollege.edu> > Content-Type: text/plain; charset=us-ascii > > Hi > > Anyone know how to open the cover of a Reichert 820 microtome? > > This is the model that has a wheel for the coarse advance on the left side. > > I am used to the AO Style, pop the latch & tip it back. These usually have a crank and a cut out slot on the left. > > This one uses 4 screws from the bottom to secure the lid and there is no cut out for the coarse advance to slide through. Looks like the wheel has to be removed to remove the cover. Getting the wheel off is where I am stuck. > > This is an old microtome we have had sitting around, would like to check the guts and clean it up. > > Jon > > > Jonathan Krupp > Applied Science, Business & Technology > San Joaquin Delta College > 5151 Pacific Ave. > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 6 Dec 2012 18:32:49 +0000 > From: Joanne Clark > Subject: [Histonet] DAKO Her2 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <0494A7D4E8CC254EA2FB81464982E37894637A37@S10MAILD001N4.SH10.lan> > Content-Type: text/plain; charset="us-ascii" > > > Hi All, sorry for how I am submitting this but I have been sending in questions to the address provided by nothing is going through. > I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. We want to start running this on our Leica BOND and any info would be much appreciated. > > Thanks > Joanne Clark, HT(ASCP) > Histology Supervisor > Pathology Consultants of New Mexico > Roswell, NM > > > > ------------------------------ > > Message: 3 > Date: Thu, 06 Dec 2012 15:28:50 -0500 > From: Tim Wheelock > Subject: [Histonet] Embedding Centers > To: histonet@lists.utsouthwestern.edu > Message-ID: <50C10002.8070708@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and > the Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, > or other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a lot. > But I would like to find a machine with a specimen holding tank large > enough to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but > embed with Surgipath Embedding Media > So I let the cassettes sit immersed in the Surgipath for an hour or two > before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves > as a third processing station, since my Shandon Hypercenter has only 2 > wax reservoirs) > I also do not feel comfortable having the cassettes sitting dry in the > holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > > > ------------------------------ > > Message: 4 > Date: Thu, 6 Dec 2012 13:41:28 -0700 > From: "O'Donnell, Bill" > Subject: RE: [Histonet] Embedding Centers > To: "Tim Wheelock" , > > Message-ID: > <4940DF6D1C5FDF48931B6966AAEF93958EF23C@chimsx08.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > Tim - Tissue-Tek 5 has been trouble-free for the 4 years we have had it > with one exception. Early on something went goofy with the dispensing > mechanism. Sakura fixed it promptly and it has been great ever since. - > Bill > > William (Bill) O'Donnell, HT (ASCP) QIHC > Senior Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > SERENITY is not freedom from the storm, but peace amid the storm. > > Cultivate it in PRAYER! > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim > Wheelock > Sent: Thursday, December 06, 2012 2:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding Centers > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and > the Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, > or other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a > lot. > But I would like to find a machine with a specimen holding tank large > enough to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but > embed with Surgipath Embedding Media So I let the cassettes sit immersed > in the Surgipath for an hour or two before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves > as a third processing station, since my Shandon Hypercenter has only 2 > wax reservoirs) I also do not feel comfortable having the cassettes > sitting dry in the holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > > > ------------------------------ > > Message: 5 > Date: Thu, 6 Dec 2012 15:43:09 -0500 > From: Andrew Coleman > Subject: [Histonet] Paraformaldehyde Solution Recipe for Perfusion > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all, > > We are performing transcardial perfusions in rats using paraformaldehyde in > 0.1M potassium phosphate buffer. > > Can anyone think of any issues that would be caused by using phosphate > buffer made from solely potassium salts (basic and dibasic), rather than a > mixture of sodium and potassium or only the sodium salts? We do our rinse > with 0.1 M PB + Saline and then follow up with solutions just made up in > the potassium phosphate buffer (therefore no Na+). > > Could this cause any tonicity/osmolarity issues? We are trying to > troubleshoot some issues we are having with the perfused tissue. > > Thanks! - Andrew > > > ------------------------------ > > Message: 6 > Date: Thu, 6 Dec 2012 16:21:54 -0500 > From: Jay Lundgren > Subject: Re: [Histonet] automated microtomes > To: Lynette Pavelich > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Leica is making the sweetest 'tomes out there at the moment, IMHO. > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > > On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich wrote: > >> I am purchasing my second Leica RM2255 automated microtome. Rene' is >> correct in saying that the carpal tunnel syndrome will eventually affect >> every advanced tech by using any microtome manually. On the RM2255, the >> flywheel additionally is automated. You can choose to use the flywheel >> manually or automated by the simultaneous pushing of two buttons. Nice >> feature for us "oldies" to ease us into automation or if you have a tiny >> specimen that you need to take extra care with and want to use manually. >> Companies are very happy to send in a demo to try for a couple weeks. >> >> Happy shopping!! ;) >> Lynette >> >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu [ >> histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [ >> rjbuesa@yahoo.com] >> Sent: Tuesday, December 04, 2012 1:19 PM >> To: Rathborne, Toni; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] automated microtomes >> >> The advantage of the so called automated microtomes (the only thing >> automated about them is the block advance) is that they alleviate wrist >> effort and in some ways prevent carpal tunnel syndrome that affects some >> histotechs (mostly of the "senior persuasion"). >> I would go with the Leica. >> Ren? J. >> >> From: "Rathborne, Toni" >> To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, December 4, 2012 11:48 AM >> Subject: [Histonet] automated microtomes >> >> I'm looking for some opinions about the automated microtomes currently >> available. Which ones do most techs prefer? Which are more reliable? Is >> there an advantage to having a semi-automated microtome? >> Thanks in advance for your replies. >> >> Toni >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 7 > Date: Thu, 6 Dec 2012 21:21:58 +0000 > From: "Burton, Lynn" > Subject: RE: [Histonet] Embedding Centers > To: Tim Wheelock , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Both animal disease labs in the state of Illinois have used the Sakura machines for the twenty years I have been here with great success. We also have aSakura processor that has been going for 25 years and a coverslipper that has only had 3 service calls for minor problems in the past 15+ years. They make good products. > Lynn Burton > Animal Disease Lab > Galesburg, Il > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock > Sent: Thursday, December 06, 2012 2:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding Centers > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, or other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a lot. > But I would like to find a machine with a specimen holding tank large enough to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but embed with Surgipath Embedding Media So I let the cassettes sit immersed in the Surgipath for an hour or two before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves as a third processing station, since my Shandon Hypercenter has only 2 wax reservoirs) I also do not feel comfortable having the cassettes sitting dry in the holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Thu, 6 Dec 2012 21:47:18 +0000 > From: "Rathborne, Toni" > Subject: RE: [Histonet] automated microtomes > To: "'Jay Lundgren'" , Lynette Pavelich > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3AD061FE740D464FAC7BF6B5CFB757071F1F6772@smcmail02.somerset-healthcare.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Thanks all for your valued opinions. I have contacted Leica, and am now waiting for a demo! > > From: Jay Lundgren [mailto:jaylundgren@gmail.com] > Sent: Thursday, December 06, 2012 4:22 PM > To: Lynette Pavelich > Cc: Rene J Buesa; Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] automated microtomes > > Leica is making the sweetest 'tomes out there at the moment, IMHO. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich > wrote: > I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. > Companies are very happy to send in a demo to try for a couple weeks. > > Happy shopping!! ;) > Lynette > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbuesa@yahoo.com] > Sent: Tuesday, December 04, 2012 1:19 PM > To: Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] automated microtomes > > The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). > I would go with the Leica. > Ren? J. > > From: "Rathborne, Toni" > > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, December 4, 2012 11:48 AM > Subject: [Histonet] automated microtomes > > I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? > Thanks in advance for your replies. > > Toni > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Thu, 6 Dec 2012 15:40:47 -0700 > From: "Patsy Ruegg" > Subject: [Histonet] refurbished histology equipment > To: > Message-ID: <311FA59E257C47B99E7C538F1DB384AE@DESKTOP3> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have a favorite vendor they would recommend to me? I am mostly > looking for cryostats right now, but interested in other things, tissue > processors, IHC stainers, etc. > > > > Patsy Ruegg, HT(ASCP)QIHC > > Ruegg IHC Consulting, LLC > > 40864 Arkansas Ave > > Bennett, CO 80102 > > Phone: 303-644-4538 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > > > > > ------------------------------ > > Message: 10 > Date: Thu, 6 Dec 2012 20:58:10 -0500 > From: Sheila Adey > Subject: [Histonet] Alcian Blue > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? > Thanks > Sheila > > ------------------------------ > > Message: 11 > Date: Fri, 7 Dec 2012 02:04:37 -0600 > From: > Subject: RE: [Histonet] Alcian Blue > To: , > Message-ID: > <4BF03F5404EBDE409AF9232DA74B9DED2DF8521C13@FWDCWPMSGCMS09.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > We've always used 30 minutes and it has never failed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey > Sent: Thursday, December 06, 2012 8:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? > Thanks > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Fri, 7 Dec 2012 12:06:15 +0000 > From: Lynette Pavelich > Subject: RE: [Histonet] Alcian Blue > To: "susan.walzer@hcahealthcare.com" , > "sadey@hotmail.ca" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <89F4666A496DC949A819ECC40E11C867BF56C8FB@EXCHANGEMB1.hmc.hurleymc.com> > > Content-Type: text/plain; charset="us-ascii" > > 30 here..... > > Lynette > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Susan.Walzer@HCAHealthcare.com [Susan.Walzer@HCAHealthcare.com] > Sent: Friday, December 07, 2012 3:04 AM > To: sadey@hotmail.ca; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Alcian Blue > > We've always used 30 minutes and it has never failed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey > Sent: Thursday, December 06, 2012 8:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? > Thanks > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 13 > Date: Fri, 7 Dec 2012 06:11:50 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Alcian Blue > To: Sheila Adey , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1354889510.81416.YahooMailNeo@web163105.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them "in house" check the preparation date and prepare a fresh solution. The general procedure calls for 30 min, unless you use a microwave oven version of the staining protocol. > Ren? J. > > From: Sheila Adey > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, December 6, 2012 8:58 PM > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? > Thanks > Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 14 > Date: Fri, 07 Dec 2012 10:50:09 -0500 > From: Geoff > Subject: Re: [Histonet] Paraformaldehyde Solution Recipe for Perfusion > To: histonet@lists.utsouthwestern.edu > Message-ID: <50C21031.7060405@umdnj.edu> > Content-Type: text/plain; CHARSET=US-ASCII; format=flowed > > What issues are you having? That might help the list diagnose the problem. > > Geoff > > On 12/6/2012 3:43 PM, Andrew Coleman wrote: >> Hi all, >> >> We are performing transcardial perfusions in rats using paraformaldehyde in >> 0.1M potassium phosphate buffer. >> >> Can anyone think of any issues that would be caused by using phosphate >> buffer made from solely potassium salts (basic and dibasic), rather than a >> mixture of sodium and potassium or only the sodium salts? We do our rinse >> with 0.1 M PB + Saline and then follow up with solutions just made up in >> the potassium phosphate buffer (therefore no Na+). >> >> Could this cause any tonicity/osmolarity issues? We are trying to >> troubleshoot some issues we are having with the perfused tissue. >> >> Thanks! - Andrew >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > > > ------------------------------ > > Message: 15 > Date: Fri, 07 Dec 2012 12:44:11 -0500 > From: Tim Wheelock > Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION > To: Histonet@lists.utsouthwestern.edu > Message-ID: <50C22AEB.6070508@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi All: > > First, thank you for all your feedback on the processors. > > I site-visited a VIP6 at a local hospital > From my understanding the VIP6 can rotate absolute alcohol and xylene > (using the bulk reservoirs), as well as the paraffin stations, but it > cannot rotate other concentrations of alcohol based on hydrometer readings. > Am I correct in this appraisal? > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 109, Issue 9 > **************************************** From Dorothy.L.Webb <@t> HealthPartners.Com Fri Dec 7 12:10:38 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Dec 7 12:10:55 2012 Subject: [Histonet] question(s) Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4301FF92B7B152@HPEMX3.HealthPartners.int> How does everyone handle storing extra paraffin sections that are cut as a standard on certain protocols, such as prostate needle bx's? We currently place them on a slide and save until the case is signed out. I am concerned with the amount of waste and cost with the way we are doing it and would like other ideas. If you keep the ribbons laid out on paper, do you stack the sheets of paper and is that problematic when it comes time to need that certain cut for IHC? Also, please let me know of those who have the VIP 6, how you like it, pros and cons and also the Leica ASP300S. Would greatly appreciate all feedback on both requests and thank you so very much!! Dorothy Webb , HT(ASCP) Regions Histology Technical Specialist ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From cpyse <@t> x-celllab.com Fri Dec 7 13:23:12 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Dec 7 13:23:45 2012 Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION In-Reply-To: <50C22AEB.6070508@mclean.harvard.edu> References: <50C22AEB.6070508@mclean.harvard.edu> Message-ID: <002201cdd4b0$4d1e0cc0$e75a2640$@com> Yes you are. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, December 07, 2012 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION Hi All: First, thank you for all your feedback on the processors. I site-visited a VIP6 at a local hospital From my understanding the VIP6 can rotate absolute alcohol and xylene (using the bulk reservoirs), as well as the paraffin stations, but it cannot rotate other concentrations of alcohol based on hydrometer readings. Am I correct in this appraisal? Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Dec 7 14:28:06 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 7 14:28:12 2012 Subject: [Histonet] question(s) In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4301FF92B7B152@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4301FF92B7B152@HPEMX3.HealthPartners.int> Message-ID: <1354912086.20629.YahooMailNeo@web163103.mail.bf1.yahoo.com> The sectioning protocols/sequences are determined by the pathologists and what they think is required to make diagnoses. We also stored the slides with extras sections until the case is signed and get an additional OK from the signing?pathologist before disposing of the unused sections. I would never ever place ribbons of sections on paper. That is calling for "disaster" in the event that some sections are required. If you think there is too much extra work and materials wastes your only solution is to ask the pathologists to review their requirements as to extra sections, specially those the pathologist may want to use for IHC. In some protocols the pathologists require automated IHC procedures before signing the case. All these issues are to be resolved by the pathologists. If the excess work is too much, you can always talk with your manager to get help regarding the protocols. Ren? J. From: "Webb, Dorothy L" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, December 7, 2012 1:10 PM Subject: [Histonet] question(s) How does everyone handle storing extra paraffin sections that are cut as a standard on certain protocols, such as prostate needle bx's?? We currently place them on a slide and save until the case is signed out.? I am concerned with the amount of waste and cost with the way we are doing it and would like other ideas.? If you keep the ribbons laid out on paper, do you stack the sheets of paper and is that problematic when it comes time to need that certain cut for IHC? Also, please let me know of those who have the VIP 6, how you like it, pros and cons and also the Leica ASP300S. Would greatly appreciate all feedback on both requests and thank you so very much!! Dorothy Webb , HT(ASCP) Regions Histology Technical Specialist ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Fri Dec 7 14:30:09 2012 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Fri Dec 7 14:30:52 2012 Subject: [Histonet] Twort's Gram Message-ID: <8C36045F0065CE48906E684F15FD4CB60A37C8@EXMBX2010-6.campus.MCGILL.CA> We are having no end of trouble with our Twort's Gram. Can someone share protocol, tip and tricks Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From joelleweaver <@t> hotmail.com Sat Dec 8 06:26:04 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Dec 8 06:26:08 2012 Subject: [Histonet] Alcian Blue In-Reply-To: References: Message-ID: AB is a basic dye that is water soluble, it reacts with anionic groups. The "classic" manual methods I checked use 30 minutes incubation times. What cells is your pathologist wanting to highlight, mucins? The general method uses acetic, and the methods for sulfated AMS, uses 1 N HCL. I guess we need to know if you are doing the general method and automated or manual- It might be a pH issue as much as the staining times. What is your control tissue? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sadey@hotmail.ca > To: histonet@lists.utsouthwestern.edu > Date: Thu, 6 Dec 2012 20:58:10 -0500 > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? > Thanks > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sat Dec 8 07:40:30 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Dec 8 07:40:33 2012 Subject: [Histonet] Alcian Blue In-Reply-To: References: Message-ID: I think the key phrase was that the pathologists expected a "small amount of cells to stain" in the esophagus biopsy. If your control has a lot of acid mucopolysaccharides (AMP) in it (such as using a normal intestine, or lung with bronchus), then 10 minutes in Alcian blue would definitely show positivity in the goblet cells or mucin cells, of which there are a lot of goblet cells in the intestine and mucin cells in the bronchus, and each goblet/mucin cell has a lot of AMP. If on the other hand, there are only a few cells, and particularly if each of the cells has a minimum amount of AMP (common in dysplastic or cancerous conditions), then 30 minutes in Alcian Blue would be better to demonstrate minimal number of cells or minimal amounts of AMP. Use 30 minutes staining time for Alcian blue all the time, to help find the minimal number of cells and/or minimal amounts of AMP. Peggy A. Wenk, HTL(ASCP) Beaumont Hospital Royal Oak, MI 48073 Opinions expressed do not reflect upon Beaumont Hospital. -----Original Message----- From: Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Mon Dec 10 08:25:07 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Dec 10 08:25:20 2012 Subject: [Histonet] moleculer testing Message-ID: <002901cdd6e2$27644c90$762ce5b0$@com> Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From marktarango <@t> gmail.com Mon Dec 10 09:01:38 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Dec 10 09:01:45 2012 Subject: [Histonet] moleculer testing In-Reply-To: <002901cdd6e2$27644c90$762ce5b0$@com> References: <002901cdd6e2$27644c90$762ce5b0$@com> Message-ID: Hi Cynthia, We do PCR for KRAS and BRAF. You cant do these by FISH anyway, the mutations are too small. We do FISH for ALK and HER2. For ALK, there is no antibody that can detect all the positive cases. ALK FISH is the only method that can detect ALK break aparts regardless of the fusion partner for ALK. If you want to do PCR for ALK, you will most likely be testing mRNA which isnt too stable and since all the ALK mutations are not known, you will miss some. This could require more tissue too. For HER2 there are some chromogenic ISH methods. I still like FISH since you can look at each color chanel individually. I never worry that one color is hiding the other and you can see each signal (no estimation of # of signals in a cluster). I dont think having a permanent record on a slide is the most important thing for molecular testing. Mark On Monday, December 10, 2012, Cynthia Pyse wrote: > Happy Monday Histonetter > > Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and > HER2neu and what testing methods are you using. I prefer not to use FISH. > Would rather have a permanent record of the slides. Any information is > welcome. Have a great week. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 20 Northpointe Parkway Suite 100 > > Amherst, NY 14228 > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Mon Dec 10 09:13:45 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Dec 10 09:13:56 2012 Subject: AW: [Histonet] moleculer testing In-Reply-To: <002901cdd6e2$27644c90$762ce5b0$@com> References: <002901cdd6e2$27644c90$762ce5b0$@com> Message-ID: <000901cdd6e8$f35086b0$d9f19410$@gmx.at> Hi, this histolab does BRAF, KRAS, EGFR with pyrosequencing and pcr; her2-amplification with DDISH on Ventana-Benchmark and ALK with FISH. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cynthia Pyse Gesendet: Montag, 10. Dezember 2012 15:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] moleculer testing Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Mon Dec 10 09:23:33 2012 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Mon Dec 10 09:23:43 2012 Subject: [Histonet] Chandler AZ HT Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C70E82E7@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for a Histotechnicians' in a brand new laboratory! Tri- City Colo-Rectal Surgery in Chandler, Arizona is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens' * HT ASCP Certified , minimum 3 years working in a histology laboratory , 1-2 years supervisor experience preferred Duties include: * Creation and maintenance of policies and procedures * Manage laboratory budget and supplies * Maintenance of QC documents' * Routine histology duties This is a full-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From twheelock <@t> mclean.harvard.edu Mon Dec 10 09:54:11 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Mon Dec 10 09:54:18 2012 Subject: [Histonet] Follow up question for embedding centers Message-ID: <50C605A3.7060608@mclean.harvard.edu> Hi Everyone: I was wondering if anyone has had experience using two other embedding centers that I have received information on? One is the Medite VALIDA. The second is the HistoPro 150. Also, is a stainless steel cold plate and/or work surface susceptible to rust down the road or not? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From vtolley <@t> cox.net Mon Dec 10 10:48:15 2012 From: vtolley <@t> cox.net (vtolley@cox.net) Date: Mon Dec 10 10:48:27 2012 Subject: [Histonet] Her2 Dual ISH and breast processing Message-ID: <20121210114815.IDQH3.63392.imail@fed1rmwml114> Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened. We are now getting breast sections that are consistently cut between 2-3mm in thickness. However, we are still having issues with inconsistency in our dual ISH staining. Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor. Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val From rjbuesa <@t> yahoo.com Mon Dec 10 11:23:41 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 10 11:23:50 2012 Subject: [Histonet] Her2 Dual ISH and breast processing In-Reply-To: <20121210114815.IDQH3.63392.imail@fed1rmwml114> References: <20121210114815.IDQH3.63392.imail@fed1rmwml114> Message-ID: <1355160221.57916.YahooMailNeo@web163102.mail.bf1.yahoo.com> Val: Acknowledging that you have a fixation problem is the fundamental step to solving your "staining" problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room?temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent?bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP?will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on?greater or?lower fat contents of the samples). Ren? J. From: "vtolley@cox.net" To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra.? Right now, we know we have a problem with our large breast tissue being under-fixed.? There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened.? We are now getting breast sections that are consistently cut between 2-3mm in thickness.? However, we are still having issues with inconsistency in our dual ISH staining.? Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor.? Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results?? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help!? Val _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMaslanka <@t> stpetes.org Mon Dec 10 11:41:27 2012 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Mon Dec 10 11:41:52 2012 Subject: [Histonet] Space requirements for AP lab Message-ID: Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain From dshaffer <@t> wellspan.org Mon Dec 10 12:39:56 2012 From: dshaffer <@t> wellspan.org (Shaffer, Dawn) Date: Mon Dec 10 12:40:32 2012 Subject: [Histonet] Qualification in Immunohistochemistry Exam Preparation Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C0159C52AA8@EXCH02.wellspan.org> Just wanted to send out thanks to histonet for a tip regarding a new study workbook available for the QIHC certification exam! After studying on my own for many months with little direction, I purchased this guide through the Michigan Society of Histotechnologists (www.mihisto.org) and found it to be extremely helpful. It is a workbook which you actually have to fill out, not something with all the answers already in it. This forces you to really research, analyze and think of all the different approaches when it comes to troubleshooting your stains. I would highly recommend it to anyone preparing for the QIHC exam! Dawn R. Shaffer, B.S. HTL (ASCP) QIHC Wellspan Health - York Hospital Anatomic Pathology Phone: (717) 851-5006 Fax: (717) 851-5114 dshaffer@wellspan.org CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From joelleweaver <@t> hotmail.com Mon Dec 10 12:46:16 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Dec 10 12:46:21 2012 Subject: [Histonet] Qualification in Immunohistochemistry Exam Preparation In-Reply-To: <6D7752544B308D44A902C0BD0EC7BF5C0159C52AA8@EXCH02.wellspan.org> References: <6D7752544B308D44A902C0BD0EC7BF5C0159C52AA8@EXCH02.wellspan.org> Message-ID: I agree Dawn, the Michigan Society has put out some great publications over the years. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: dshaffer@wellspan.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 10 Dec 2012 13:39:56 -0500 > Subject: [Histonet] Qualification in Immunohistochemistry Exam Preparation > > Just wanted to send out thanks to histonet for a tip regarding a new study workbook available for the QIHC certification exam! After studying on my own for many months with little direction, I purchased this guide through the Michigan Society of Histotechnologists (www.mihisto.org) and found it to be extremely helpful. It is a workbook which you actually have to fill out, not something with all the answers already in it. This forces you to really research, analyze and think of all the different approaches when it comes to troubleshooting your stains. I would highly recommend it to anyone preparing for the QIHC exam! > > > Dawn R. Shaffer, B.S. HTL (ASCP) QIHC > Wellspan Health - York Hospital > Anatomic Pathology > Phone: (717) 851-5006 > Fax: (717) 851-5114 > dshaffer@wellspan.org > > > > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . > > ______________________________________________________________________ > This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Dec 10 13:19:00 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Dec 10 13:19:06 2012 Subject: AW: [Histonet] Her2 Dual ISH and breast processing In-Reply-To: <20121210114815.IDQH3.63392.imail@fed1rmwml114> References: <20121210114815.IDQH3.63392.imail@fed1rmwml114> Message-ID: <000301cdd70b$362949a0$a27bdce0$@gmx.at> I recommend to make a "tumour-block" right at the first time of grossing. This block is put immediatly into NBF and is optimal fixed for the next day. The rest of the breast is sliced up as usual and fixed over night. Next day "tumour-block" and rest is processed together. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von vtolley@cox.net Gesendet: Montag, 10. Dezember 2012 17:48 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened. We are now getting breast sections that are consistently cut between 2-3mm in thickness. However, we are still having issues with inconsistency in our dual ISH staining. Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor. Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From krendelm <@t> upstate.edu Mon Dec 10 15:24:55 2012 From: krendelm <@t> upstate.edu (Mira Krendel) Date: Mon Dec 10 15:24:09 2012 Subject: [Histonet] Problems with cryosectioning snap-frozen mouse kidney Message-ID: <126318CF-D261-4657-B00B-DF33024AB928@upstate.edu> Hello Histonet members, I was wondering if you could provide some input on a problem we have encountered recently. We routinely section mouse kidneys for IF. The kidneys are cut in half and snap-frozen in Tissue-Tek cryomold in OCT using isopentane/dry ice bath. Lately we have been having problems with tissue not sectioning properly (i.e., the OCT will section fine but when the technician gets to the tissue itself, the blade starts "chattering", creating multiple folds). We have tried a different cryostat and had a similar issue. Someone has mentioned to me that this problem may be caused by not using cryoprotection with sucrose prior to freezing, although we have not had issues with sectioning snap-frozen tissue before. Any advice on how to troubleshoot this? Thanks in advance for your time, Mira Mira Krendel SUNY Syracuse, NY From Krendelm <@t> upstate.edu Mon Dec 10 16:38:59 2012 From: Krendelm <@t> upstate.edu (Mira Krendel) Date: Mon Dec 10 16:38:25 2012 Subject: [Histonet] Re: Cryosectioning mouse kidney problems Message-ID: <3861647B-473E-4DA2-94D3-343A285328FA@upstate.edu> Thank you, Belinda and Gayle, for your suggestions. Sounds like I will need to double-check the temperature at which the sectioning was done. The tissue is fresh (we used to fix with PFA and then cryoprotect with sucrose but we have switched to using freshly frozen kidneys (snap frozen in OCT) several years ago. Have not really had any major issues until now so I will need to check whether any settings on the (shared) cryostat have been changed recently. We use disposable blades; the latest attempt was done with a high profile blade on a different cryostat but with the same issues. Thanks again, Mira _______________________ I have questions since mouse kidney is generally easy to cryosection without problems. You never said whether you prefix with NBF or PFA or only use fresh tissue prior to snap freezing? You CANNOT cryoprotect fresh tissue or you will have excessive swelling of the tissue. Sucrose cryoprotection can only be done with NBF or PFA fixed tissue to prevent large ice crystal freezing artifact. You also did NOT say what temperature you were using for cryosectioning? Please clarify. You also did not indicate what kind of knife or disposable blade you are using? The sharper the better. I suspect you are sectioning too cold, so try -20C, the blade and block temperature must be exactly the same temperature. If you go from the cold e.g. freezing platform on most cryostats directly to sectioning, the tissue will be too cold and chatter when blade passes through tissue. Cold freezing platforms are often 10 to 20 degrees colder than the desired cutting temperature. Let the blocks with tissue equilibrate off the platform, near the mictorome itself. We measure the hot and cold spots in our cryostat to know where we can equilibrate the tissue to sectioning temperature. Your blade holder could be loose, the blade angle off a bit and or the blade is getting dull. High profile disposable blades are superior for cryotomy, more stable and just as sharp as low profile. If you are using steel knives, do you use a sharp, NEW edge for sectioning. My hopes are you use disposable blades. It is important to section the kidney at an angle, so you don't go across the broadest part of the kidney first. If you do the latter, chatter will occur as the disposable blade meets with resistance that can be felt when blade edge engages the tissue. Don't cut to thick as 5 um is ideal. Double check your blade angle/tight screws/sharp blade. Ahhhh the fine details. Gayle M. Callis HTL/HT/MT(ASCP) _______________________ You will not need sucrose if it is frozen unfixed but it does sound like the tissue froze too fast/ too cold Try warming the tissue with your thumb just prior to cutting a section and see if that helps B From eyee <@t> dpmginc.com Mon Dec 10 17:26:55 2012 From: eyee <@t> dpmginc.com (Ellen Yee) Date: Mon Dec 10 17:27:05 2012 Subject: [Histonet] workbook for preparation for the QIHC Message-ID: <20121210232655.c0d43855@mail.dpmginc.com> Hi, I tried the Michigan Society for Histotechnologists, but when I clicked on the workbook, was unable to get anything. How can I get in touch with them? Ellen Yee Diagnostic Pathology Medical Group Sacramento, CA 916-448-5873 eyee@dpmginc.com From lpwenk <@t> sbcglobal.net Mon Dec 10 19:27:51 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Dec 10 19:27:54 2012 Subject: [Histonet] workbook for preparation for the QIHC In-Reply-To: <20121210232655.c0d43855@mail.dpmginc.com> References: <20121210232655.c0d43855@mail.dpmginc.com> Message-ID: <2C38A8AAC3AC42E88CED84912D4939B8@HP2010> Towards the bottom of the MSH webpage on study guides available through MSH, is a link for the application form. www.mihisto.org click on Education click on Study Guides Peggy Wenk -----Original Message----- From: Ellen Yee Sent: Monday, December 10, 2012 6:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] workbook for preparation for the QIHC Hi, I tried the Michigan Society for Histotechnologists, but when I clicked on the workbook, was unable to get anything. How can I get in touch with them? Ellen Yee Diagnostic Pathology Medical Group Sacramento, CA 916-448-5873 eyee@dpmginc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khairedai <@t> yahoo.com Mon Dec 10 22:09:37 2012 From: khairedai <@t> yahoo.com (Khaire Dai) Date: Mon Dec 10 22:09:42 2012 Subject: [Histonet] (no subject) Message-ID: <1355198977.19478.YahooMailNeo@web124706.mail.ne1.yahoo.com> http://pengwangfp.com/wp-content/plugins/akismet/google.html From girishcm <@t> gmail.com Mon Dec 10 23:00:02 2012 From: girishcm <@t> gmail.com (girish cm) Date: Mon Dec 10 23:00:06 2012 Subject: [Histonet] Enquiry about blood removal from frozen tissue sections Message-ID: Hello, I would like to know whether any methods (other than cold PBS wash) or products are available to remove the blood components from frozen tissue sections. My application is antibody targeted spectroscopic imaging in which the blood components are providing background noise. Thanking you, Regards -- Girish C M Senior Research Fellow Amrita Centre for Nanoscience & Molecular Medicine Amrita Institute of Medical Sciences & Research Centre Cochin, Kerala India-682 041 Ph: 9645095045 From SHUNTER <@t> beaumont.edu Tue Dec 11 06:37:15 2012 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Dec 11 06:37:24 2012 Subject: [Histonet] Enquiry about blood removal from frozen tissue sections In-Reply-To: References: Message-ID: You could try hydrogen peroxide - it wil get rid of the heme in the RBCs which is probably giving you the background. I would try a 1:10 dilution (or higher) of the H2O2 (in PBS) that you usually get from the store/pharmacy - undiluted will probably bubble too much and lift off the tissue. You will have to try dilutions and time - usually 10 minutes is good, but with a diluted solution you may need to go longer. Then wash the slides in PBS to get rid of the H2O2. Good Luck Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of girish cm Sent: Tuesday, December 11, 2012 12:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Enquiry about blood removal from frozen tissue sections Hello, I would like to know whether any methods (other than cold PBS wash) or products are available to remove the blood components from frozen tissue sections. My application is antibody targeted spectroscopic imaging in which the blood components are providing background noise. Thanking you, Regards -- Girish C M Senior Research Fellow Amrita Centre for Nanoscience & Molecular Medicine Amrita Institute of Medical Sciences & Research Centre Cochin, Kerala India-682 041 Ph: 9645095045 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Tue Dec 11 09:55:23 2012 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Tue Dec 11 09:55:42 2012 Subject: [Histonet] Recall: Node Issue Message-ID: <3855827CD3E36249A30D57F6F896F8F1745836CB@EXMBX2.aruplab.net> Metzger, Kenneth would like to recall the message, "Node Issue". ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From kenneth.metzger <@t> aruplab.com Tue Dec 11 09:56:28 2012 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Tue Dec 11 09:56:37 2012 Subject: [Histonet] Recall: Node Issue Message-ID: <3855827CD3E36249A30D57F6F896F8F1745836D4@EXMBX2.aruplab.net> Metzger, Kenneth would like to recall the message, "Node Issue". ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From sdysart <@t> mirnarx.com Tue Dec 11 13:26:35 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Dec 11 13:26:47 2012 Subject: [Histonet] Pathologist Consult Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D503896E002@BL2PRD0710MB363.namprd07.prod.outlook.com> Does anyone know of any good services that do a per slide pathology read out? I would prefer a veterinary pathologist and it would not be a very large case load. Mainly tox. related reports would be what we need generated. Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From sadey <@t> hotmail.ca Tue Dec 11 14:10:25 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Tue Dec 11 14:10:29 2012 Subject: [Histonet] Inking esophagus biopsies Message-ID: Hi Everyone:Can anyone reccommend a good way to ink esophageal biopsies without using mercurochrome? ThanksSheila From mpence <@t> grhs.net Tue Dec 11 14:16:15 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Dec 11 14:16:47 2012 Subject: [Histonet] Inking esophagus biopsies In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974F3F@is-e2k3.grhs.net> I use eosin in a squirt bottle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Tuesday, December 11, 2012 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inking esophagus biopsies Hi Everyone:Can anyone reccommend a good way to ink esophageal biopsies without using mercurochrome? ThanksSheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Dec 12 12:17:02 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Dec 12 12:17:08 2012 Subject: [Histonet] Re: Inking esophagus biopsies Message-ID: Sheila Adey (somewhere in Canada) asks: Can anyone reccommend a good way to ink esophageal biopsies without using mercurochrome? Out of many pathology services I've worked on, I've only seen one that marked GI biopsies when grossing. They used safranin O (the routine Gram stain counterstain, from the microbiology lab) and it worked quite well. I would not use eosin because it makes fluorescence microscopy impossible. You can't get mercurochrome any more (in the US anyway), and you don't want the mercury in your processor anyway. Bob Richmond Samurai Pathologist Maryville TN From akbitting <@t> geisinger.edu Wed Dec 12 14:36:43 2012 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Dec 12 14:37:03 2012 Subject: [Histonet] Space requirements for AP lab In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F60BCCF481@GHSEXMBX2W8K1V.geisinger.edu> Well, if it's more than 2.5 sq. ft per tech, we're out of compliance :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Monday, December 10, 2012 12:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From lblazek <@t> digestivespecialists.com Wed Dec 12 14:54:19 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Dec 12 14:56:16 2012 Subject: [Histonet] Space requirements for AP lab In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F60BCCF481@GHSEXMBX2W8K1V.geisinger.edu> References: <77F52EFAB8B1694B885E277C48FCD0F60BCCF481@GHSEXMBX2W8K1V.geisinger.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E391643405244@IBMB7Exchange.digestivespecialists.com> LOL I think if you have to ask then there isn't enough room! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, December 12, 2012 3:37 PM To: JMaslanka@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Well, if it's more than 2.5 sq. ft per tech, we're out of compliance :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Monday, December 10, 2012 12:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 12 15:43:27 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 12 15:43:37 2012 Subject: [Histonet] Space requirements for AP lab In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF02F355@ex07.net.ucsf.edu> Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.Hart <@t> bms.com Wed Dec 12 16:32:59 2012 From: Heather.Hart <@t> bms.com (Hart, Heather) Date: Wed Dec 12 16:32:58 2012 Subject: [Histonet] Modified Gomori Trichrome Message-ID: <55EA897EF9AC2342BD9322B86AC195B6171A686676@ushpwbmsmmp007.one.ads.bms.com> I have request to implement this stain for frozen muscle in OCT blocks. The pathologist is looking for demonstration of ragged red fibers, however it is my understanding (very limited) that this (in this circumstance) is a diagnostic stain and the tissue on hand that I will be using for development will be normal with no RRF present. I am unable to discern what type of tissue to use as a positive control, outside of muscle diseased specimen. Any suggestions and/or working methods would be greatly appreciated. Heather Hart, MLT (ASCP) ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 12 17:11:02 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 12 17:11:37 2012 Subject: [Histonet] RE: Modified Gomori Trichrome In-Reply-To: <55EA897EF9AC2342BD9322B86AC195B6171A686676@ushpwbmsmmp007.one.ads.bms.com> References: <55EA897EF9AC2342BD9322B86AC195B6171A686676@ushpwbmsmmp007.one.ads.bms.com> Message-ID: <761E2B5697F795489C8710BCC72141FF02F3A6@ex07.net.ucsf.edu> Heather, Short answer: if you do a good trichrome on normal muscle tissue you'll be fine. Ragged red fibers describe muscle that has abnormal concentrated areas of mitochondria - they show as large red areas within the muscle fiber. However, if you do a normal muscle and can see the mitochondria staining red, then you will also be able to see the mitochondrial concentrations on diseased fibers. Doing this stain well requires excellent frozen muscle tissue, which means snap freezing in methyl butane cooled by liquid nitrogen. If you haven't done that procedure I suggest reviewing this video on youtube: http://www.youtube.com/watch?v=W4Wk7iSJJZs Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hart, Heather Sent: Wednesday, December 12, 2012 2:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Modified Gomori Trichrome I have request to implement this stain for frozen muscle in OCT blocks. The pathologist is looking for demonstration of ragged red fibers, however it is my understanding (very limited) that this (in this circumstance) is a diagnostic stain and the tissue on hand that I will be using for development will be normal with no RRF present. I am unable to discern what type of tissue to use as a positive control, outside of muscle diseased specimen. Any suggestions and/or working methods would be greatly appreciated. Heather Hart, MLT (ASCP) ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Thu Dec 13 06:04:51 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Dec 13 06:04:58 2012 Subject: [Histonet] Space requirements for AP lab In-Reply-To: <761E2B5697F795489C8710BCC72141FF02F355@ex07.net.ucsf.edu> References: , <761E2B5697F795489C8710BCC72141FF02F355@ex07.net.ucsf.edu> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56CEF7@EXCHANGEMB1.hmc.hurleymc.com> We are Joint Commission. I've never run across this issue (and God forbid bringing it up in front of one!) in 40+ years and 3 lab renovations. CAP people out there?? Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, December 12, 2012 4:43 PM To: JMaslanka@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From W.E.J.Hoekert <@t> olvg.nl Thu Dec 13 06:35:43 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Dec 13 06:35:50 2012 Subject: [Histonet] Her2 Dual ISH and breast processing References: <20121210114815.IDQH3.63392.imail@fed1rmwml114> <1355160221.57916.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F842B5@PAIT42.olvg.nl> I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying to convince our pathologists about the importance of good fixation, but the pathologist are always in a hurry to get the diagnosis out. Many mamma biopsies do not get to be fixed long enough (some are even put in the processor after a few hours of fixation). It would be helpfull if I can show them some literature to back me up. Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtolley@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental step to solving your "staining" problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). Ren? J. From: "vtolley@cox.net" To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened. We are now getting breast sections that are consistently cut between 2-3mm in thickness. However, we are still having issues with inconsistency in our dual ISH staining. Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor. Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From jqb7 <@t> cdc.gov Thu Dec 13 06:54:44 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Dec 13 06:55:01 2012 Subject: [Histonet] Space requirements for AP lab In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56CEF7@EXCHANGEMB1.hmc.hurleymc.com> References: , <761E2B5697F795489C8710BCC72141FF02F355@ex07.net.ucsf.edu> <89F4666A496DC949A819ECC40E11C867BF56CEF7@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: Years ago I worked in a small, community hospital. The histology lab was TINY! very well organized and tidy...but TINY! Every inspection we got cited for being too small. Can't remember which agency but we were a teaching hospital......... Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 13, 2012 7:05 AM To: Morken, Timothy; jmaslanka@stpetes.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab We are Joint Commission. I've never run across this issue (and God forbid bringing it up in front of one!) in 40+ years and 3 lab renovations. CAP people out there?? Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, December 12, 2012 4:43 PM To: JMaslanka@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Thu Dec 13 08:05:19 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Thu Dec 13 08:05:24 2012 Subject: [Histonet] VIP6 Questions Message-ID: <50C9E09F.2060001@mclean.harvard.edu> Good morning Everyone: For those of you who have a Sakura VIP6, do you actually use the bulk reservoirs to let the machine automatically rotate the absolute alcohols and xylene? If not, why not? Also do you let the machine automatically rotate the paraffin reservoirs, or do you do this manually, and if so, why? Also, do you find the touch screen graphics easy to use? Have any problems developed with the display itself? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From Nancy_Schmitt <@t> pa-ucl.com Thu Dec 13 08:17:24 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Dec 13 08:17:38 2012 Subject: [Histonet] VIP6 issue Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B07@PEITHA.wad.pa-ucl.com> Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rmweber113 <@t> comcast.net Thu Dec 13 08:26:14 2012 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Dec 13 08:26:23 2012 Subject: [Histonet] Path Lab Assistant Job In-Reply-To: <1629028778.697845.1355408106829.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> Message-ID: <1859191934.698277.1355408774511.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> Northeast Philadelphia?physician in office path laboratory is looking for a laboratory assistant.? Must have strong computer skills.? Duties include data entry, phones,?inventory control, filing of slides,?and miscellaneous tasks. 15 to 20 hours per week. Please fax resume to 215 947-2015 attention Laboratory Manager. ? From abuchiane <@t> bmhvt.org Thu Dec 13 09:06:48 2012 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Thu Dec 13 09:07:01 2012 Subject: [Histonet] Synoptic Reporting Message-ID: <4034E71604330C4B8E10D1538DFB2455059E3B@BMHEXCH02.bmhvt.org> What are people doing for synoptic reporting? Are the CAP templates the only option? _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From brendal.finlay <@t> medicalcenterclinic.com Thu Dec 13 09:32:07 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Thu Dec 13 09:32:13 2012 Subject: [Histonet] VIP6 issue Message-ID: <9b1dbd897c5bfa8a0555c255473ac685@medicalcenterclinic.com> I've always used a lid on the basket when using the VIP.? If we did not do so, the blocks will float around, but we have not experienced cassette lids coming off. Is the lid or rack something in addition to a lid being on the basket already? Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.finlay@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -----Original message----- From: Nancy Schmitt Nancy_Schmitt@pa-ucl.com Date: Thu, 13 Dec 2012 09:17:24 -0600 To: "Histonet (histonet@lists.utsouthwestern.edu)"histonet@lists.utsouthwestern.edu Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lidon top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 13 09:47:13 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 13 09:47:18 2012 Subject: [Histonet] Her2 Dual ISH and breast processing In-Reply-To: <1190CB05C44B13409483514729C2FC3601F842B5@PAIT42.olvg.nl> References: <20121210114815.IDQH3.63392.imail@fed1rmwml114> <1355160221.57916.YahooMailNeo@web163102.mail.bf1.yahoo.com> <1190CB05C44B13409483514729C2FC3601F842B5@PAIT42.olvg.nl> Message-ID: <1355413633.5858.YahooMailNeo@web163106.mail.bf1.yahoo.com> Please go to http://www.histosearch.co/rene.html There are 2 articles on?the subject:?one?about the general fixation?issue and another about the minimum amount of NBF required to obtain complete fixation. Ren? J. From: "Hoekert, W.E.J." To: Rene J Buesa ; vtolley@cox.net; histonet@lists.utsouthwestern.edu Sent: Thursday, December 13, 2012 7:35 AM Subject: RE: [Histonet] Her2 Dual ISH and breast processing I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying to convince our pathologists about the importance of good fixation, but the pathologist are always in a hurry to get the diagnosis out. Many mamma biopsies do not get to be fixed long enough (some are even put in the processor after a few hours of fixation). It would be helpfull if I can show them some literature to back me up. Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtolley@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental step to solving your "staining" problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). Ren? J. From: "vtolley@cox.net" To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra.? Right now, we know we have a problem with our large breast tissue being under-fixed.? There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened.? We are now getting breast sections that are consistently cut between 2-3mm in thickness.? However, we are still having issues with inconsistency in our dual ISH staining.? Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor.? Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results?? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From rjbuesa <@t> yahoo.com Thu Dec 13 09:50:17 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 13 09:50:22 2012 Subject: [Histonet] Her2 Dual ISH and breast processing In-Reply-To: <1355413633.5858.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <20121210114815.IDQH3.63392.imail@fed1rmwml114> <1355160221.57916.YahooMailNeo@web163102.mail.bf1.yahoo.com> <1190CB05C44B13409483514729C2FC3601F842B5@PAIT42.olvg.nl> <1355413633.5858.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <1355413817.81630.YahooMailNeo@web163104.mail.bf1.yahoo.com> My mistake: the web site is: http://www.histosearch.com/rene.html From: Rene J Buesa To: "Hoekert, W.E.J." ; "vtolley@cox.net" ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, December 13, 2012 10:47 AM Subject: Re: [Histonet] Her2 Dual ISH and breast processing Please go to http://www.histosearch.co/rene.html There are 2 articles on?the subject:?one?about the general fixation?issue and another about the minimum amount of NBF required to obtain complete fixation. Ren? J. From: "Hoekert, W.E.J." To: Rene J Buesa ; vtolley@cox.net; histonet@lists.utsouthwestern.edu Sent: Thursday, December 13, 2012 7:35 AM Subject: RE: [Histonet] Her2 Dual ISH and breast processing I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying to convince our pathologists about the importance of good fixation, but the pathologist are always in a hurry to get the diagnosis out. Many mamma biopsies do not get to be fixed long enough (some are even put in the processor after a few hours of fixation). It would be helpfull if I can show them some literature to back me up. Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtolley@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental step to solving your "staining" problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). Ren? J. From: "vtolley@cox.net" To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra.? Right now, we know we have a problem with our large breast tissue being under-fixed.? There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened.? We are now getting breast sections that are consistently cut between 2-3mm in thickness.? However, we are still having issues with inconsistency in our dual ISH staining.? Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor.? Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results?? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Thu Dec 13 09:51:17 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Dec 13 09:51:26 2012 Subject: [Histonet] VIP6 issue Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B52@PEITHA.wad.pa-ucl.com> Sorry for the confusion - the cassettes lids are not coming off. The lid for the rack which came with the new processor get off kilter during processing allowing the cassettes to float up out of the rack. Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 13 10:48:20 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 13 10:48:34 2012 Subject: [Histonet] Space requirements for AP lab In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56CEF7@EXCHANGEMB1.hmc.hurleymc.com> References: , <761E2B5697F795489C8710BCC72141FF02F355@ex07.net.ucsf.edu> <89F4666A496DC949A819ECC40E11C867BF56CEF7@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <761E2B5697F795489C8710BCC72141FF02F7E5@ex07.net.ucsf.edu> It's not a theoretical question. When I came here I found our EM lab / Muscle histochem Lab / IF lab are crammed into one small space. It had 30years of accumulated stuff that no one wanted to throw out. There were microscope tables in the middle of walkways, the histochem staining area is the primary path into the lab - and the walkway itself is inadequately sized - imagine crowding through when staining is going on! JC inspectors cited the lab for inadequate working space. That put focus on a lab that had been largely ignored and we spent a long while "decluttering", surplussing unused equipment and getting a new, larger, fume hood. (Thank you JC!!) That said, I think it is a very subjective judgment by an inspector and I am not aware of any specific space requirements that any agency applies beyond "adequate." In this case it was one inspector out of dozens that had been through the lab previously. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Lynette Pavelich [mailto:LPaveli1@hurleymc.com] Sent: Thursday, December 13, 2012 4:05 AM To: Morken, Timothy; jmaslanka@stpetes.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab We are Joint Commission. I've never run across this issue (and God forbid bringing it up in front of one!) in 40+ years and 3 lab renovations. CAP people out there?? Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, December 12, 2012 4:43 PM To: JMaslanka@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.harvey <@t> Vanderbilt.Edu Thu Dec 13 11:03:25 2012 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Thu Dec 13 11:03:51 2012 Subject: [Histonet] Ubiquitin and alpha synuclein Message-ID: What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 13 11:03:35 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 13 11:04:55 2012 Subject: [Histonet] RE: VIP6 issue In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B07@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B07@PEITHA.wad.pa-ucl.com> Message-ID: <761E2B5697F795489C8710BCC72141FF02F81F@ex07.net.ucsf.edu> " I talked with the rep. and they said they had never heard of this." Right. Yes, it happens. Often. Too much air caught in the cassettes. Worse with the fine-mesh cassettes. Bounce the racks in the formalin tray a few times before loading on the processor to try to get as much air out as possible. That will improve processing as well. And weight down the tops if it continues. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 13, 2012 6:17 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 13 11:12:03 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 13 11:12:17 2012 Subject: [Histonet] RE: Ubiquitin and alpha synuclein In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF02F870@ex07.net.ucsf.edu> Jennifer, Thermo/Lab Vision has some excellent Synuclein antibodies. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harvey, Jennifer Lynn Sent: Thursday, December 13, 2012 9:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ubiquitin and alpha synuclein What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Dec 13 11:18:36 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Dec 13 11:18:40 2012 Subject: [Histonet] RE: Ubiquitin and alpha synuclein In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF03048A@UWHC-MBX12.uwhis.hosp.wisc.edu> Jennifer, We use p62/SQSTM1 from MBL (Medical & Biological Laboratories, Co., LTD), code M162-3, as our Ubiquitin and alpha Synuclein from Invitrogen, #18-0215. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harvey, Jennifer Lynn Sent: Thursday, December 13, 2012 11:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ubiquitin and alpha synuclein What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Thu Dec 13 11:38:47 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Dec 13 11:38:51 2012 Subject: [Histonet] VIP6 questions In-Reply-To: <20121213170715.EDF611AA036@mail.pa-ucl.com> References: <20121213170715.EDF611AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1BF1@PEITHA.wad.pa-ucl.com> We do not use the bulk reservoirs at this time - our storeroom is 6 feet away. We do automatically rotate paraffin and love it! Yes - we find the touch screen easy to use. No problems have developed, but it is still very new. Would you share your opinions on these questions? Nancy Schmitt United Clinical Laboratories Dubuque, IA -------------------------------------------------------------------------------------- Message: 10 Date: Thu, 13 Dec 2012 09:05:19 -0500 From: Tim Wheelock Subject: [Histonet] VIP6 Questions To: Histonet@lists.utsouthwestern.edu Message-ID: <50C9E09F.2060001@mclean.harvard.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Good morning Everyone: For those of you who have a Sakura VIP6, do you actually use the bulk reservoirs to let the machine automatically rotate the absolute alcohols and xylene? If not, why not? Also do you let the machine automatically rotate the paraffin reservoirs, or do you do this manually, and if so, why? Also, do you find the touch screen graphics easy to use? Have any problems developed with the display itself? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From o.m.gallagher <@t> sheffield.ac.uk Thu Dec 13 11:44:04 2012 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Thu Dec 13 11:44:15 2012 Subject: [Histonet] Maximum bone sample size for methyl methacrylate embedding Message-ID: Dear Histonetters, Would anyone advise on the maximum size sample of undecalcified bone which could be properly processed into methyl methacrylate for sectioning and staining for Goldner's trichrome? Would anyone have a protocol for processing large bone samples (possibly 2 x1cm) into MMA as most of the protocols I've seen are based on Bordier trephine 5mm iliac crest biopsies. Thank you, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX Website: http://mellanbycentre.dept.shef.ac.uk Tel: 00353114-2713337 (office) 00353114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. From PAMarcum <@t> uams.edu Thu Dec 13 11:44:43 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Dec 13 11:44:53 2012 Subject: [Histonet] Histologists Needed in Little Rock AR Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA329604866D@Mail2Node2.ad.uams.edu> We are still looking for two ASCP registered Histologists for a busy University Histology Laboratory at UAMS. We are in the heart of Arkansas in a beautiful area with lots to do and see all year around. This is a modern Histology Laboratory that is growing, expanding with more expansion and equipment acquisitions for the future. Due to the rules required by the state of Arkansas, we at the University of Arkansas for Medical Sciences we are not allowed to use recruiters for any position so please do not contact me for placements of any kind. Also due to the state regulations we cannot pay sign on bonuses or moving expenses at this time. I am attempting to be honest so you know what we can and cannot do before contacting me if you are interested. Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From LPaveli1 <@t> hurleymc.com Thu Dec 13 11:44:48 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Dec 13 11:44:59 2012 Subject: [Histonet] RE: VIP6 issue In-Reply-To: <761E2B5697F795489C8710BCC72141FF02F81F@ex07.net.ucsf.edu> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B07@PEITHA.wad.pa-ucl.com>, <761E2B5697F795489C8710BCC72141FF02F81F@ex07.net.ucsf.edu> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56CFAA@EXCHANGEMB1.hmc.hurleymc.com> Tim brings up a good point. I would monitor the processing in the fine mesh type cassettes as well. For years, and I do mean years, we struggled with our tiny specimens. Why did some specimens in mesh cassettes do just fine, some not....it was an absolute nightmare! Monitored how the specimen was handled starting right at the sight of surgery on through changing processing schedules/times/heat?/no heat/xylene/xylene substitute/staining schedules. We wet the mesh cassettes prior to loading, then trying to swish the air pocket out of the cassette before processing. (did not experience the lids coming off) We found that the only thing that has produced consistently good results was when we went back to the lens paper. What we think was going on, was that the little biopsies would intermittently get caught up in a air bubble during processing (agitation, pumping in/out), thus "missing" being in that current solution(s). It truly was a nightmare.......... We now purchase cheap lens paper, and cut it into 4 squares......paper cutter does a large amount quickly and we're good to go. Yes, it is a little more hassle unwrapping them in the morning, but if it gives a better quality specimen then it doesn't matter!! Just my two cents worth........;) Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, December 13, 2012 12:03 PM To: Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: VIP6 issue " I talked with the rep. and they said they had never heard of this." Right. Yes, it happens. Often. Too much air caught in the cassettes. Worse with the fine-mesh cassettes. Bounce the racks in the formalin tray a few times before loading on the processor to try to get as much air out as possible. That will improve processing as well. And weight down the tops if it continues. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 13, 2012 6:17 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Dec 13 12:35:18 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Dec 13 12:35:24 2012 Subject: [Histonet] RE: VIP6 issue In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56CFAA@EXCHANGEMB1.hmc.hurleymc.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B07@PEITHA.wad.pa-ucl.com>, <761E2B5697F795489C8710BCC72141FF02F81F@ex07.net.ucsf.edu> <89F4666A496DC949A819ECC40E11C867BF56CFAA@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: One of the products I learned about at the 2012 NSH convention was ActivFlo tissue cassettes. Designed by JB McCormick (I think) they are biopsy cassettes that have side vents to help disperse the trapped air bubbles. Here is a link from Leica, they sell several different ActiveFlo cassettes... http://www.leicabiosystems.com/products/consumables/cassettes-base-molds/biopsy-cassettes/ Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 13, 2012 12:45 PM To: Morken, Timothy; Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: VIP6 issue Tim brings up a good point. I would monitor the processing in the fine mesh type cassettes as well. For years, and I do mean years, we struggled with our tiny specimens. Why did some specimens in mesh cassettes do just fine, some not....it was an absolute nightmare! Monitored how the specimen was handled starting right at the sight of surgery on through changing processing schedules/times/heat?/no heat/xylene/xylene substitute/staining schedules. We wet the mesh cassettes prior to loading, then trying to swish the air pocket out of the cassette before processing. (did not experience the lids coming off) We found that the only thing that has produced consistently good results was when we went back to the lens paper. What we think was going on, was that the little biopsies would intermittently get caught up in a air bubble during processing (agitation, pumping in/out), thus "missing" being in that current solution(s). It truly was a nightmare.......... We now purchase cheap lens paper, and cut it into 4 squares......paper cutter does a large amount quickly and we're good to go. Yes, it is a little more hassle unwrapping them in the morning, but if it gives a better quality specimen then it doesn't matter!! Just my two cents worth........;) Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, December 13, 2012 12:03 PM To: Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: VIP6 issue " I talked with the rep. and they said they had never heard of this." Right. Yes, it happens. Often. Too much air caught in the cassettes. Worse with the fine-mesh cassettes. Bounce the racks in the formalin tray a few times before loading on the processor to try to get as much air out as possible. That will improve processing as well. And weight down the tops if it continues. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 13, 2012 6:17 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From madeleinehuey <@t> gmail.com Thu Dec 13 12:37:24 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Dec 13 12:37:32 2012 Subject: [Histonet] Re: Histonet Digest, Vol 109, Issue 14 In-Reply-To: <50ca0ab2.648db60a.27fd.ffff9dbbSMTPIN_ADDED_MISSING@mx.google.com> References: <50ca0ab2.648db60a.27fd.ffff9dbbSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Val, Go to CAP website (http://www.cap.org/apps/cap.portal) and you will find the answer to your question, or give them a call @ 800-323-4040. HER2 have a very specific guide line for clinical test. My understanding the fixation should be minimun 6 hrs & maximum 48 hrs. Your problem could be resolved if you have a good rapid tissue processor (ie. Leica - Peloris, etc). We have the same problem with VIP5 before I purchased the Peloris. I haven't have one single re-process since then. There is a trick to my problem. Give me an email if you want to know. Madeleine Huey, HTL & QIHC (ASCP) madeleine_h@elcaminohospital.com On Thu, Dec 13, 2012 at 9:04 AM, wrote: > Her2 Dual ISH and breast processing From SteveM <@t> mcclainlab.com Thu Dec 13 13:54:26 2012 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Thu Dec 13 13:53:51 2012 Subject: [Histonet] VIP6 issue Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF3056EBBB@ML1.McClainLabs.local> I may be oversimplifying, but I see that as one of many reasons for controlling fixation on grossing bench, Then rinsing in alcohol and starting in alcohol on the processor. 50% Alcohol has less surface tension than water and fewer air bubbles. Blue sponges have a number of annoying artifacts including air bubbles and floating cassettes. And are to be avoided in my opinion. Agree w wrapping small specimens in 50mmx50mm lens paper. Jamming cassettes together in processor baskets 20 to a row worsens air-bubble problem. May I also suggest spreading cassettes out to facilitate diffusion? Not more than 2 high. VIP6 must have a progressive basket design. All older models used a simple weighted basket top. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From ddreesen <@t> sbcglobal.net Thu Dec 13 14:08:55 2012 From: ddreesen <@t> sbcglobal.net (Debbie Dreesen) Date: Thu Dec 13 14:08:59 2012 Subject: [Histonet] Re: VIP6 issue14 Message-ID: <1355429335.68307.YahooMailClassic@web182206.mail.bf1.yahoo.com> Nancy, Like you, we had that?problem and had to put something on top to prevent the cassettes from floating around. ? Debbie Dreesen ? Message: 11 Date: Thu, 13 Dec 2012 14:17:24 +0000 From: Nancy Schmitt Subject: [Histonet] VIP6 issue To: "Histonet (histonet@lists.utsouthwestern.edu)" ??? Message-ID: ??? <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C1B07@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Good Morning- We have recently purchased? VIP6 processors.? Has anyone else experienced a problem with the lids coming off during pump in and pump out?? Causes the cassettes to float all over and be completely out of order:(? We now place an extra rack or lid on top to weigh down and insure this does not happen.? I talked with the rep. and they said they had never heard of this.? I know this is not? a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From W.E.J.Hoekert <@t> olvg.nl Fri Dec 14 08:37:05 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Dec 14 08:39:08 2012 Subject: [Histonet] Her2 Dual ISH and breast processing References: <20121210114815.IDQH3.63392.imail@fed1rmwml114><1355160221.57916.YahooMailNeo@web163102.mail.bf1.yahoo.com><1190CB05C44B13409483514729C2FC3601F842B5@PAIT42.olvg.nl><1355413633.5858.YahooMailNeo@web163106.mail.bf1.yahoo.com> <1355413817.81630.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F842B9@PAIT42.olvg.nl> Thank you for the references, I have found what I needed in this article: Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma. Goldstein NS, et. al., Am J Clin Pathol 2003;120:86-92 Willem >My mistake: the web site is: http://www.histosearch.com/rene.html >From: Rene J Buesa >To: "Hoekert, W.E.J." ; "vtolley@cox.net" ; >"histonet@lists.utsouthwestern.edu" > [....] >Willem, yes, there are. Look through the attached references which include some for >breast fixation. There have been some more recently as well, just go on >scholar.google.com . > > >Tim Morken >Supervisor, Electron Microscopy/Neuromuscular Special Studies >Department of Pathology >UC San Francisco Medical Centero Medical Center Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From hans <@t> histologistics.com Fri Dec 14 09:39:58 2012 From: hans <@t> histologistics.com (Hans B Snyder) Date: Fri Dec 14 09:40:03 2012 Subject: [Histonet] Does Formalin fixed frozen sections for antibodies need to be kept frozen? Message-ID: Hello I am working at a research facility that has thousands of formalin fixed frozen heart sections in their freezers. They have been led to believe that this is necessary for their antibodies to work. I do not believe this is the case. Does anyone know of antibodies that will lose their antigenicity if stored at room temp? Thanks -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com From Timothy.Morken <@t> ucsfmedctr.org Fri Dec 14 10:16:33 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Dec 14 10:16:44 2012 Subject: [Histonet] Does Formalin fixed frozen sections for antibodies need to be kept frozen? In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF02FB8E@ex07.net.ucsf.edu> Hans, There are definitely certain antigens that will be degraded when the tissue is stored as tissue sections on slides, even if formalin-fixed. Other antigens will not be degraded even after years of storage. It is very specific per antigen. People have tried various methods to reduce or stop this degradation including freezing, paraffin coating the entire slide, storage in nitrogen atmosphere or combinations. It really depends on what the particular researcher has found in their previous work, so I would not dismiss the freezing out hand. Maybe ask if they have some documentation (ie, who "led" them to believe this?!?!) of the time to degradation for particular antigens (ie, evidence of lack of antibody labeling after a certain time period). Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: Friday, December 14, 2012 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does Formalin fixed frozen sections for antibodies need to be kept frozen? Hello I am working at a research facility that has thousands of formalin fixed frozen heart sections in their freezers. They have been led to believe that this is necessary for their antibodies to work. I do not believe this is the case. Does anyone know of antibodies that will lose their antigenicity if stored at room temp? Thanks -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Dec 14 10:54:29 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Dec 14 10:54:39 2012 Subject: [Histonet] Synoptic Reporting In-Reply-To: <4034E71604330C4B8E10D1538DFB2455059E3B@BMHEXCH02.bmhvt.org> References: <4034E71604330C4B8E10D1538DFB2455059E3B@BMHEXCH02.bmhvt.org> Message-ID: <06c301cdda1b$b00d3480$10279d80$@pathview.com> Good morning Anita, Here's a response from an IT vendor perspective: While I am by no means an expert at the interpretation of CAP, CMS, or any of the other myriad of regulations that we must comply with, it's always been my understanding that the use of synoptic templates was somewhat of a 'grey' area. My take on things is that you don't 'have' to use the actual CAP templates, but you must ensure that the questions in those templates are addressed in your patient report. We have certain sites that would rather not pay CAP's cost to use their templates, so we've given them the ability to create their own templates. Depending on your LIS vendor, perhaps you have this ability. If not, there is no reason why you couldn't use WORD or Dragon or whatever word processing tool you possess to create templates or autocorrect entries that ensure that the required questions are addressed. There are other aspects of this question that could be dealt with, but I'll leave that up for further discussion should the group, or yourself, wish to entertain them. Off the top of my head, I think of sending discrete data/answer elements to other systems for research or federal funding purposes. There's also the issue of which agency you are trying to comply with, CAP vs cancer institute vs others. I?m anxious to hear other people's take on this subject as I think we will see a continual increased use and requirement for synoptic reporting. Bottom line, I see a day when all surgical reports will be required to be resulted/reported in a synoptic fashion. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Thursday, December 13, 2012 7:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Synoptic Reporting What are people doing for synoptic reporting? Are the CAP templates the only option? _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Dec 14 11:00:04 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 14 11:00:11 2012 Subject: [Histonet] Does Formalin fixed frozen sections for antibodies need to be kept frozen? In-Reply-To: References: Message-ID: <1355504404.15507.YahooMailNeo@web163101.mail.bf1.yahoo.com> The antigen in?sections, either from FFPE or frozen, kept at room temperature will lose their signal in 1-2 weeks. The thing is that you do not need to formalin fix frozen sections. I have kept FS at -80?C for many years and have used them successfully in antigen detection. Further more, those FS do not need HIER because they were not formalin fixed and the antigens have not been cross-linked. A different thing will be?if the FS were done on already formalin fixed tissues, although that is not frequently done. In the event that the FS were from formalin fixed tissues they will need HIER but probably you will lose a great majority of the sections during the process. Because of that antigen in FS should be "reactivated" either enzymatically or do HIER at?low temp during more time. Ren? J. From: Hans B Snyder To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 10:39 AM Subject: [Histonet] Does Formalin fixed frozen sections for antibodies need to be kept frozen? Hello I am working at a research facility that has thousands of formalin fixed frozen heart sections in their freezers.? They have been led to believe that this is necessary for their antibodies to work.? I do not believe this is the case.? Does anyone know of antibodies that will lose their antigenicity if stored at room temp? Thanks -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Fri Dec 14 14:37:25 2012 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Fri Dec 14 14:37:34 2012 Subject: [Histonet] AZ HT Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C70F4636@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Tri- City Colo-Rectal Surgery is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified , minimum 3 years working in a histology laboratory , 1-2 years supervisor experience preferred Duties include: * Creation and maintenance of policies and procedures * Manage laboratory budget and supplies * Maintenance of QC documents' * Routine histology duties This is a full-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From ttroyer <@t> petersonlab.com Fri Dec 14 16:34:03 2012 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Fri Dec 14 16:34:08 2012 Subject: [Histonet] Basis for Quality Work in a Histotech Message-ID: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer From rjbuesa <@t> yahoo.com Sat Dec 15 10:38:05 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 15 10:38:11 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> Message-ID: <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to?ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me?that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of?experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there?is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J.???? From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors.? I am the supervisor of two histotechs.? I am not doing techwork now, but have 10 years of experience.? The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening.? I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal.? For example,? if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame.? We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihisto <@t> hotmail.com Sat Dec 15 16:43:48 2012 From: brandihisto <@t> hotmail.com (Brandi Farris) Date: Sat Dec 15 16:43:53 2012 Subject: [Histonet] Job opening in Jefferson City, MO Message-ID: Hello All, I have an opening for a tech in Jefferson City, MO. Great hospital, flexible hours, no nights/weekends/holidays. Please apply at the attached link. Thanks, Brandi Farris https://api.crmc.org/ApplicantsNavigator/ReqDetail.aspx?reqid=1028 From rsrichmond <@t> gmail.com Sun Dec 16 10:50:37 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Dec 16 10:50:42 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech Message-ID: Travis Troyer at Peterson Laboratory Servcies in Manhattan, Kansas (Hey, one of my grandfathers was born there!) asks: >>This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution.<< Well, speaking as a small-lab pathologist, what I'd want to do is tell you exactly what the "lack of quality" and the "mistakes" I'm seeing are. Some of them, like specimen mixups and errors in accessioning, would be your problem to get right. With problems like bad microtomy and faulty staining, I'd want to look at a lot of slides with you (a double-headed microscope is needed for this purpose, and I'm guessing you haven't got one). Then I'd want you to look at those slides with your two histotechs. This daily process would go on for a long time, perhaps permanently. If you were out of the office, I'd want to do the daily review directly with one of the histotechs. I hold the perhaps naive belief that people will do good work if they have good equipment and know what's expected of them and get feedback (both positive and negative. I'd a lot rather tell a histotech "Hey, you really pulled this patient's diagnosis out of the fire for me" than "This GI biopsy slide is so chattery I can't interpret it.") One of the great follies of Good Management is that people are pairs of hands who just need to be made to work-to-rule. If you don't know who Edwards Deming was, look him up in Wikipedia. Bob Richmond Samurai Pathologist Maryville TN From joelleweaver <@t> hotmail.com Sun Dec 16 13:29:03 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sun Dec 16 13:29:07 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: References: Message-ID: Good suggestion, Deming's PDCA is a good, straightforward framework to begin with. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sun, 16 Dec 2012 11:50:37 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Basis for Quality Work in a Histotech > > Travis Troyer at Peterson Laboratory Servcies in Manhattan, Kansas > (Hey, one of my grandfathers was born there!) asks: >>This is a > question for all of the lab supervisors. I am the supervisor of two > histotechs. I am not doing techwork now, but have 10 years of > experience. The pathologists are getting more and more upset at the > lack of quality in the work and the mistakes that are happening. I was > wondering if anyone had some ideas on what sort of a goal to set up > and how to reward/punish for variations from that goal. For example, > if the goal is three mistakes for the month, what is the best way to > reward them for making that goal and what would be best if they had > more mistakes in a given time frame. We are all feeling the budget > crunch and the pathologists are trying to figure out a good > solution.<< > > Well, speaking as a small-lab pathologist, what I'd want to do is tell > you exactly what the "lack of quality" and the "mistakes" I'm seeing > are. Some of them, like specimen mixups and errors in accessioning, > would be your problem to get right. With problems like bad microtomy > and faulty staining, I'd want to look at a lot of slides with you (a > double-headed microscope is needed for this purpose, and I'm guessing > you haven't got one). Then I'd want you to look at those slides with > your two histotechs. This daily process would go on for a long time, > perhaps permanently. If you were out of the office, I'd want to do the > daily review directly with one of the histotechs. > > I hold the perhaps naive belief that people will do good work if they > have good equipment and know what's expected of them and get feedback > (both positive and negative. I'd a lot rather tell a histotech "Hey, > you really pulled this patient's diagnosis out of the fire for me" > than "This GI biopsy slide is so chattery I can't interpret it.") > > One of the great follies of Good Management is that people are pairs > of hands who just need to be made to work-to-rule. If you don't know > who Edwards Deming was, look him up in Wikipedia. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Annette_hall <@t> pa-ucl.com Sun Dec 16 23:23:48 2012 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Sun Dec 16 23:23:52 2012 Subject: [Histonet] Chemical Inventory/MSDS Program Message-ID: <8B8724C83737DF41888FFD6ECCB161B121F4B597@PEITHA.wad.pa-ucl.com> All, We currently are using an internally developed program to track chemical inventory and accompanying MSDS sheets. This application requires extensive resources to maintain an accurate inventory. We are looking to replace it with a commercial solution. Could you please share what solution your laboratory or hospital is using as a resource for reagent/chemical handling? Additional thoughts about ease of use, accuracy, accessibility, maintenance resources, etc., are greatly appreciated. Thanks, Annette Annette J Hall, MT Micro/Histo/Cyto Manager United Clinical Laboratories 205 Bluff St. Dubuque, IA 52001 563.556.2010 x131 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Sharon.Genest <@t> saskatoonhealthregion.ca Mon Dec 17 06:55:41 2012 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Mon Dec 17 06:55:55 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech Message-ID: My first recommendation would be to look at your process is there any way that you can error proof them? Make it more difficult to make the errors.When a lot of errors are occuring sometimes it is often due to how we do the job and not who does it. Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca his e-mail message may contain confidential and/or privileged information. It is intended only for the addressee. Any unauthorized disclosure is strictly prohibited. If you are not a named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmissions cannot be guaranteed to be secure or error free as information could be intercepted, corrupted, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept any liability for errors or omissions in the contents of this message or any damages that arise as a result of e-mail transmissions. From cmiller <@t> physlab.com Mon Dec 17 08:59:29 2012 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Dec 17 08:59:40 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: I totally agree with you Rene. May I add as a supervisor of 6 in which all of them I trained all myself in an OJT situation. 1. Instill in them team work and pride in their profession 2. Reiterate to them that their unique skills are an important link in the health care chain. If they lack any of above it's your job to provide them with the knowledge, skill and understanding of their critical role in the health care chain. My belief is you will see a rise in quality with minimal mistakes. If they don?t improve after receiving all of the above they should look for another profession. Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 10:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J. From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From LPaveli1 <@t> hurleymc.com Mon Dec 17 09:28:44 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Dec 17 09:28:52 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: References: Message-ID: <89F4666A496DC949A819ECC40E11C867BF56D1C2@EXCHANGEMB1.hmc.hurleymc.com> Travis, If it were me (and I've done this), I would go back to the bench and walk in their shoes to see what is really happening. Come in at the start of their shift and work right along side them. This way, you can see who/what/when is going on: How are the machines being maintained (how often/quality of maintenance .....is the 95% really 95%, etc.) How is the processing schedule? Does it need "tweeking"? How is the embedding? Quality? Go ahead and embed some. How is the cutting? Quality/quantity? Go ahead and cut some. How is the routine staining? Maintenance good? Times good? How are the special stains? Are protocols being followed or does each "cook" have their own recipe they follow? How is your procedure manual(s) Does it need cleaning up? Does the special stain manual contain pictures of what a good stain should look like? Get my drift? Lots of things to think about. I would go on the bench for a week and see what really happens every day. It may be intimidating at first, but it will show your techs and your pathologists how much you care and this should help your approach when helping them improve their techniques. This may need to be done a few times, and each time, you will see improvements. I don't know of any techs who want to do bad work, they just may need guidance in getting there. Additionally, it will be helpful to track what infractions are going on and the frequency (we have a monthly tracking system and I report it at our QA meetings (mislabeled slides, mislabeled blocks, etc). Also, do you have a system in place to evaluate competency? This will be helpful when evaluation time comes around to approach them in areas of needed improvement. If you need help in developing a competency evaluation, the Michigan Society (www.mihisto.org) has a manual for supervisors that contains many different styles of evaluations that includes the different goals, measurements, assessment frequency, references and resources to help you develop an evaluation unique to your institution. Well worth the $5.00 investment. Supervising at times, can be a tough job, but I can tell you really care about the patient. The best of luck to you! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Genest, Sharon SktnHR [Sharon.Genest@saskatoonhealthregion.ca] Sent: Monday, December 17, 2012 7:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Basis for Quality Work in a Histotech My first recommendation would be to look at your process is there any way that you can error proof them? Make it more difficult to make the errors.When a lot of errors are occuring sometimes it is often due to how we do the job and not who does it. Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca his e-mail message may contain confidential and/or privileged information. It is intended only for the addressee. Any unauthorized disclosure is strictly prohibited. If you are not a named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmissions cannot be guaranteed to be secure or error free as information could be intercepted, corrupted, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept any liability for errors or omissions in the contents of this message or any damages that arise as a result of e-mail transmissions. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Dec 17 10:03:03 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Dec 17 10:03:07 2012 Subject: [Histonet] RELIA Special Job Alert for Supervisors and Managers 12-17-2012 Message-ID: <06e001cddc6f$ff1529b0$fd3f7d10$@earthlink.net> Hello Histonetters!! I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations in California. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: Pathology Supervisor - Fresno, CA Histology Supervisor - Los Angeles, CA Histology Manager - Los Angeles, CA I anticipate multiple management openings coming available nationwide after the holidays. If you are interested in looking into a change in another area of the country please let me know. That way I can keep you apprised of management opportunities in that area. Also if you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 10 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Happy Holidays !! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mtighe <@t> trudeauinstitute.org Mon Dec 17 10:20:11 2012 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Dec 17 10:20:21 2012 Subject: [Histonet] Human bcell and Tcell Ab Message-ID: <108215434A378A4E8246C567E79DD4811CEB1E9C@CH1PRD0711MB407.namprd07.prod.outlook.com> Hi Everybody! Can you guys weigh in on the best antibodies for Human B cell and Tcell staining in FFPE sections? We would like to have a fluorescent endpoint if that changes things. Thanks in advance! Hope everyone has a safe Holiday. Mike From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 17 10:30:23 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Dec 17 10:30:38 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to?ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me?that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of?experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there?is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J.???? From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors.? I am the supervisor of two histotechs.? I am not doing techwork now, but have 10 years of experience.? The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening.? I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal.? For example,? if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame.? We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Mon Dec 17 10:57:39 2012 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Dec 17 10:57:46 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> Message-ID: <1355763459.39171.YahooMailNeo@web122002.mail.ne1.yahoo.com> Tim, I'd be interested in more information in your labeling at the microtome that has all but eliminated errors. Would you share? ? Thank you, Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, December 17, 2012 11:30 AM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why?? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to?ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me?that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of?experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there?is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J.???? From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors.? I am the supervisor of two histotechs.? I am not doing techwork now, but have 10 years of experience.? The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening.? I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal.? For example,? if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame.? We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Mon Dec 17 11:11:44 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Dec 17 11:11:52 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <1355763459.39171.YahooMailNeo@web122002.mail.ne1.yahoo.com> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu>, <1355763459.39171.YahooMailNeo@web122002.mail.ne1.yahoo.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56D279@EXCHANGEMB1.hmc.hurleymc.com> V291bGQgYWxzbyBsb3ZlIHRvIGhlYXIhICQkIGZvciBiYXIgY29kaW5nIHRvbyBmYXIgYXdheSEN Cg0KTHluZXR0ZQ0KDQpMeW5ldHRlIFBhdmVsaWNoLCBIVChBU0NQKQ0KSGlzdG9sb2d5IFN1cGVy dmlzb3INCkh1cmxleSBNZWRpY2FsIENlbnRlcg0KT25lIEh1cmxleSBQbGF6YQ0KRmxpbnQsIE1J IDQ4NTAzDQoNCnBoOiA4MTAuMjYyLjk5NDgNCm1vYmlsZTogODEwLjQ0NC43OTY2DQoNCl9fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkZyb206IGhpc3RvbmV0LWJvdW5j ZXNAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1IFtoaXN0b25ldC1ib3VuY2VzQGxpc3RzLnV0c291 dGh3ZXN0ZXJuLmVkdV0gb24gYmVoYWxmIG9mIFNoZWlsYSBIYWFzIFttaWNyb3BhdGhsYWJzQHlh aG9vLmNvbV0NClNlbnQ6IE1vbmRheSwgRGVjZW1iZXIgMTcsIDIwMTIgMTE6NTcgQU0NClRvOiBN b3JrZW4sIFRpbW90aHk7IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KU3ViamVj dDogUmU6IFtIaXN0b25ldF0gQmFzaXMgZm9yIFF1YWxpdHkgV29yayBpbiBhIEhpc3RvdGVjaA0K DQpUaW0sDQpJJ2QgYmUgaW50ZXJlc3RlZCBpbiBtb3JlIGluZm9ybWF0aW9uIGluIHlvdXIgbGFi 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DQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVk dQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3Rv bmV0DQpfX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlz dG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0 dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K DQpfX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9u ZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6 Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0KX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkhpc3RvbmV0IG1h aWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlz dHMudXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQ= From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 17 11:52:23 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Dec 17 11:52:35 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56D279@EXCHANGEMB1.hmc.hurleymc.com> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu>, <1355763459.39171.YahooMailNeo@web122002.mail.ne1.yahoo.com> <89F4666A496DC949A819ECC40E11C867BF56D279@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <761E2B5697F795489C8710BCC72141FF02FE6C@ex07.net.ucsf.edu> Here is our slide labeling procedure. It is simple, but we insist it be followed. This will be modified once barcoding is instituted to use scanning ID of slides vs block. We also instituted block ID beads at the embedding center. That has helped tremendously to identify people that may need embedding retraining. One other thing I should mention. People in the lab should be told to trust their inner voice. We had several labeling errors that on investigation were suspected or ignored by a tech in the chain or events. In one case the sectioning tech pulled the wrong block from the file, labeled the slides with that block number and sent it to the IHC group. An IHC tech took the slide and noticed the number was wrong but ASSUMED the sectioning tech had made an error, so "corrected" the number to match the request on her log. The mistake was only caught by the pathologist who noticed the IHC slide did not match the H&E slide from the proper block. I tell my techs: Trust your instincts. If something seems wrong - for whatever reason - STOP!!! Investigate the issue. You will save time, effort, and maybe lives. I also tell my techs not no pathologist will remember how fast you were, just how many mistakes you made. Tim Morken +++++++++++++++++++++++++++++++++++++++++++ Slide Labeling Procedure at the Microtome Slide labeling at the cutting station is the most important part of the process used to identify slides. A mistake at this point will cause a mix-up in slides or cases and could lead to improper diagnosis and treatment for patients. Because this is such an important step the following procedure must be followed exactly. Deviation may cause mislabeling. Deviation from the procedure may be cause for reprimand and termination. Important points: 1. Label slides for one block at a time. 2. Do not pre-label slides before cutting sections from a block, or for any other blocks. 3. Work with one block at a time as much as possible. If the block must be re-soaked before completing the slide count then the block must be isolated from the other blocks to prevent a mix-up. 4. The stainer tech will compare stained slides to the blocks to ensure they are correct. Sectioning Procedure 1) Pick up the next page of the processing log and the blocks that are on that page. 2) Verify that the blocks picked up are on the processing log. a. Put the blocks in order according to the processing log. b. Put a check mark ??? next to the block line on the log to indicate the block is present in the group. c. Note any problems with the blocks 1. Put an ?X? mark next to any problem blocks and note the problem. a. Add-ons b. Missing c. Reprocessing needed d. Re-embedding and reason e. other 3) Face-trim the blocks and put them on ice (if necessary) in order as shown on the processing log. 4) Pick up the first block on the log. a. Do not pre-label slides for this or any other block 5) Place the block in the microtome and cut sections according to a. requests on the processing log and established sectioning requirements b. ?Tissue Sectioning Standards? of the Sectioning section of the Histology Laboratory manual 6) Pick up the proper number of sections on the appropriate slides. 7) Label the slides a. Write the Accession number and Part letter/number of the block on the microtome. b. Label slides with your initials (legibly) c. Label initial slide in the group with the cassette color and page number (this slide is first in the rack and faces forward for the Stainer person to see) d. NOTE: If the block must be re-soaked to complete the slide count, then isolate the block on the ice tray by putting it at the back of the tray facing backwards. This will indicate that some slides have been cut previously. 8) Place the slides for H&E in the H&E staining rack a. Write the color and page number of the processing log on the first slide of the rack. b. Place slides in the rack beginning at the front of the rack and working backward. c. Place designated ?unstained? slides in separate rack(s) for use later (IPOX, Specials, etc). d. Discard any unused slides with sections on them. 9) Take the block from the microtome and put it in the cardboard filing box. 10) Clean the surface of the water with a Kimwipe or paper towel. Be sure there are no tissue fragments floating on the water bath. 11) Cross off that case on the processing log with a diagonal line through the entire case. 12) When the H&E slide rack is filled take it to the 60C oven a. Hand-write a label with the cassette color and page# as well as the time the slides will come out of the oven (10 min from current time). b. Place the label on the front end of the slide rack (end with first slide). c. Put the slides in the oven with the label facing forward. 13) When all the blocks have been cut, write your initials (legibly) on the bottom of the log and note the date/time. a. Compare Blocks and slides i. Give blocks to the Stainer area tech ii. When staining is completed the Stainer Tech compares the stained slides against the blocks and the process log. iii. If stained slides and blocks match, write ?Blocks/Slides match?, your initials and time at the bottom of the process log. iv. If slide(s) and block(s) do not match, determine the problem and correct it. v. Note the problem and correction on the process log along with your initials and time. b. Deliver the slides back to the Stainer Tech. 14) Place the cut blocks on the filing table. 15) Fill out a workload tally sheet for the day. -----Original Message----- From: Lynette Pavelich [mailto:LPaveli1@hurleymc.com] Sent: Monday, December 17, 2012 9:12 AM To: Sheila Haas; Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basis for Quality Work in a Histotech Would also love to hear! $$ for bar coding too far away! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, December 17, 2012 11:57 AM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech Tim, I'd be interested in more information in your labeling at the microtome that has all but eliminated errors. Would you share? Thank you, Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, December 17, 2012 11:30 AM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J. From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Kay <@t> albertahealthservices.ca Mon Dec 17 12:41:01 2012 From: Karen.Kay <@t> albertahealthservices.ca (Karen Kay) Date: Mon Dec 17 12:41:08 2012 Subject: [Histonet] BASIS FOR QUALITY WORK IN A HISTOTECH In-Reply-To: <20121217175419.74DE7E024C@buckler.calgaryhealthregion.ca> References: <20121217175419.74DE7E024C@buckler.calgaryhealthregion.ca> Message-ID: Good Morning, I am moved to add my response to this question, not only to provide an opinion on the query, but to extend a request to those who respond to any query, be it this one or others. Please do not respond in such a caustic manner as to belittle the person sending in the initial request!!! This is not a a forum for this type of remark and is just plain rude! One poses a question to the members of the Histonet expecting assistance with an operational issue and for the most part, one receives good help in this regard, Travis, An opinion to your query is to establish a Quality Assurance program which includes clear and distinct guidelines as to the expectations of your laboratory. In addition, ensure that a general/individual discussion be conducted with your staff, outlining the quality issues and the effect that they have on a patient's diagnosis. In our Laboratory (staff of 12) we will have a general discussion with all of the staff if there is a particular trend that is occurring. In addition, when required, a separate meeting with the technologist/assistant is held if required. These meetings are documented should there be any further actions taken and for subsequent staff evaluations Some of the recommendations or references you have already been given in this regard looks like they would be helpful to you. Thank you Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen.kay@albertahealthservices.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: December 17, 2012 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Re: Basis for Quality Work in a Histotech (joelle weaver) 2. Chemical Inventory/MSDS Program (Annette Hall) 3. Re: Basis for Quality Work in a Histotech (Genest, Sharon SktnHR) 4. RE: Basis for Quality Work in a Histotech (Cheri Miller) 5. RE: Re: Basis for Quality Work in a Histotech (Lynette Pavelich) 6. RELIA Special Job Alert for Supervisors and Managers 12-17-2012 (Pam Barker) 7. Human bcell and Tcell Ab (Mike Tighe) 8. RE: Basis for Quality Work in a Histotech (Morken, Timothy) 9. Re: Basis for Quality Work in a Histotech (Sheila Haas) 10. RE: Basis for Quality Work in a Histotech (Lynette Pavelich) 11. RE: Basis for Quality Work in a Histotech (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Sun, 16 Dec 2012 19:29:03 +0000 From: joelle weaver Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good suggestion, Deming's PDCA is a good, straightforward framework to begin with. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sun, 16 Dec 2012 11:50:37 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Basis for Quality Work in a Histotech > > Travis Troyer at Peterson Laboratory Servcies in Manhattan, Kansas > (Hey, one of my grandfathers was born there!) asks: >>This is a > question for all of the lab supervisors. I am the supervisor of two > histotechs. I am not doing techwork now, but have 10 years of > experience. The pathologists are getting more and more upset at the > lack of quality in the work and the mistakes that are happening. I was > wondering if anyone had some ideas on what sort of a goal to set up > and how to reward/punish for variations from that goal. For example, > if the goal is three mistakes for the month, what is the best way to > reward them for making that goal and what would be best if they had > more mistakes in a given time frame. We are all feeling the budget > crunch and the pathologists are trying to figure out a good > solution.<< > > Well, speaking as a small-lab pathologist, what I'd want to do is tell > you exactly what the "lack of quality" and the "mistakes" I'm seeing > are. Some of them, like specimen mixups and errors in accessioning, > would be your problem to get right. With problems like bad microtomy > and faulty staining, I'd want to look at a lot of slides with you (a > double-headed microscope is needed for this purpose, and I'm guessing > you haven't got one). Then I'd want you to look at those slides with > your two histotechs. This daily process would go on for a long time, > perhaps permanently. If you were out of the office, I'd want to do the > daily review directly with one of the histotechs. > > I hold the perhaps naive belief that people will do good work if they > have good equipment and know what's expected of them and get feedback > (both positive and negative. I'd a lot rather tell a histotech "Hey, > you really pulled this patient's diagnosis out of the fire for me" > than "This GI biopsy slide is so chattery I can't interpret it.") > > One of the great follies of Good Management is that people are pairs > of hands who just need to be made to work-to-rule. If you don't know > who Edwards Deming was, look him up in Wikipedia. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 17 Dec 2012 05:23:48 +0000 From: Annette Hall Subject: [Histonet] Chemical Inventory/MSDS Program To: "histonet@lists.utsouthwestern.edu" Message-ID: <8B8724C83737DF41888FFD6ECCB161B121F4B597@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" All, We currently are using an internally developed program to track chemical inventory and accompanying MSDS sheets. This application requires extensive resources to maintain an accurate inventory. We are looking to replace it with a commercial solution. Could you please share what solution your laboratory or hospital is using as a resource for reagent/chemical handling? Additional thoughts about ease of use, accuracy, accessibility, maintenance resources, etc., are greatly appreciated. Thanks, Annette Annette J Hall, MT Micro/Histo/Cyto Manager United Clinical Laboratories 205 Bluff St. Dubuque, IA 52001 563.556.2010 x131 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 3 Date: Mon, 17 Dec 2012 12:55:41 +0000 From: "Genest, Sharon SktnHR" Subject: [Histonet] Re: Basis for Quality Work in a Histotech To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" My first recommendation would be to look at your process is there any way that you can error proof them? Make it more difficult to make the errors.When a lot of errors are occuring sometimes it is often due to how we do the job and not who does it. Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca his e-mail message may contain confidential and/or privileged information. It is intended only for the addressee. Any unauthorized disclosure is strictly prohibited. If you are not a named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmissions cannot be guaranteed to be secure or error free as information could be intercepted, corrupted, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept any liability for errors or omissions in the contents of this message or any damages that arise as a result of e-mail transmissions. ------------------------------ Message: 4 Date: Mon, 17 Dec 2012 08:59:29 -0600 From: Cheri Miller Subject: RE: [Histonet] Basis for Quality Work in a Histotech To: Rene J Buesa Cc: histonet Message-ID: Content-Type: text/plain; charset="utf-8" I totally agree with you Rene. May I add as a supervisor of 6 in which all of them I trained all myself in an OJT situation. 1. Instill in them team work and pride in their profession 2. Reiterate to them that their unique skills are an important link in the health care chain. If they lack any of above it's your job to provide them with the knowledge, skill and understanding of their critical role in the health care chain. My belief is you will see a rise in quality with minimal mistakes. If they don???t improve after receiving all of the above they should look for another profession. Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 10:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ??? counsel the HT after a mistake ??? retrain them ??? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren?? J. From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 5 Date: Mon, 17 Dec 2012 15:28:44 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech To: "Genest, Sharon SktnHR" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <89F4666A496DC949A819ECC40E11C867BF56D1C2@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" Travis, If it were me (and I've done this), I would go back to the bench and walk in their shoes to see what is really happening. Come in at the start of their shift and work right along side them. This way, you can see who/what/when is going on: How are the machines being maintained (how often/quality of maintenance .....is the 95% really 95%, etc.) How is the processing schedule? Does it need "tweeking"? How is the embedding? Quality? Go ahead and embed some. How is the cutting? Quality/quantity? Go ahead and cut some. How is the routine staining? Maintenance good? Times good? How are the special stains? Are protocols being followed or does each "cook" have their own recipe they follow? How is your procedure manual(s) Does it need cleaning up? Does the special stain manual contain pictures of what a good stain should look like? Get my drift? Lots of things to think about. I would go on the bench for a week and see what really happens every day. It may be intimidating at first, but it will show your techs and your pathologists how much you care and this should help your approach when helping them improve their techniques. This may need to be done a few times, and each time, you will see improvements. I don't know of any techs who want to do bad work, they just may need guidance in getting there. Additionally, it will be helpful to track what infractions are going on and the frequency (we have a monthly tracking system and I report it at our QA meetings (mislabeled slides, mislabeled blocks, etc). Also, do you have a system in place to evaluate competency? This will be helpful when evaluation time comes around to approach them in areas of needed improvement. If you need help in developing a competency evaluation, the Michigan Society (www.mihisto.org) has a manual for supervisors that contains many different styles of evaluations that includes the different goals, measurements, assessment frequency, references and resources to help you develop an evaluation unique to your institution. Well worth the $5.00 investment. Supervising at times, can be a tough job, but I can tell you really care about the patient. The best of luck to you! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Genest, Sharon SktnHR [Sharon.Genest@saskatoonhealthregion.ca] Sent: Monday, December 17, 2012 7:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Basis for Quality Work in a Histotech My first recommendation would be to look at your process is there any way that you can error proof them? Make it more difficult to make the errors.When a lot of errors are occuring sometimes it is often due to how we do the job and not who does it. Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.genest@saskatoonhealthregion.ca his e-mail message may contain confidential and/or privileged information. It is intended only for the addressee. Any unauthorized disclosure is strictly prohibited. If you are not a named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmissions cannot be guaranteed to be secure or error free as information could be intercepted, corrupted, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept any liability for errors or omissions in the contents of this message or any damages that arise as a result of e-mail transmissions. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 17 Dec 2012 11:03:03 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Special Job Alert for Supervisors and Managers 12-17-2012 To: "Histonet" Message-ID: <06e001cddc6f$ff1529b0$fd3f7d10$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hello Histonetters!! I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations in California. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: Pathology Supervisor - Fresno, CA Histology Supervisor - Los Angeles, CA Histology Manager - Los Angeles, CA I anticipate multiple management openings coming available nationwide after the holidays. If you are interested in looking into a change in another area of the country please let me know. That way I can keep you apprised of management opportunities in that area. Also if you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 10 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Happy Holidays !! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 7 Date: Mon, 17 Dec 2012 16:20:11 +0000 From: Mike Tighe Subject: [Histonet] Human bcell and Tcell Ab To: "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Message-ID: <108215434A378A4E8246C567E79DD4811CEB1E9C@CH1PRD0711MB407.namprd07.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" Hi Everybody! Can you guys weigh in on the best antibodies for Human B cell and Tcell staining in FFPE sections? We would like to have a fluorescent endpoint if that changes things. Thanks in advance! Hope everyone has a safe Holiday. Mike ------------------------------ Message: 8 Date: Mon, 17 Dec 2012 16:30:23 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Basis for Quality Work in a Histotech To: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> Content-Type: text/plain; charset=utf-8 Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to??ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me??that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of??experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ??? counsel the HT after a mistake ??? retrain them ??? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there??is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren?? J.???????? From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors.?? I am the supervisor of two histotechs.?? I am not doing techwork now, but have 10 years of experience.?? The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening.?? I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal.?? For example,?? if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame.?? We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 17 Dec 2012 08:57:39 -0800 (PST) From: Sheila Haas Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "Morken, Timothy" , "histonet@lists.utsouthwestern.edu" Message-ID: <1355763459.39171.YahooMailNeo@web122002.mail.ne1.yahoo.com> Content-Type: text/plain; charset=utf-8 Tim, I'd be interested in more information in your labeling at the microtome that has all but eliminated errors. Would you share? ?? Thank you, Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, December 17, 2012 11:30 AM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why??? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to??ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me??that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of??experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ??? counsel the HT after a mistake ??? retrain them ??? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there??is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren?? J.???????? From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors.?? I am the supervisor of two histotechs.?? I am not doing techwork now, but have 10 years of experience.?? The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening.?? I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal.?? For example,?? if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame.?? We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 17 Dec 2012 17:11:44 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Basis for Quality Work in a Histotech To: Sheila Haas , "Morken, Timothy" , "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF56D279@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="gb2312" Would also love to hear! $$ for bar coding too far away! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, December 17, 2012 11:57 AM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech Tim, I'd be interested in more information in your labeling at the microtome that has all but eliminated errors. Would you share? Thank you, Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, December 17, 2012 11:30 AM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ?? counsel the HT after a mistake ?? retrain them ?? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren?? J. From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 17 Dec 2012 17:52:23 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Basis for Quality Work in a Histotech To: "Lynette Pavelich" , "Sheila Haas" , "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF02FE6C@ex07.net.ucsf.edu> Content-Type: text/plain; charset=utf-8 Here is our slide labeling procedure. It is simple, but we insist it be followed. This will be modified once barcoding is instituted to use scanning ID of slides vs block. We also instituted block ID beads at the embedding center. That has helped tremendously to identify people that may need embedding retraining. One other thing I should mention. People in the lab should be told to trust their inner voice. We had several labeling errors that on investigation were suspected or ignored by a tech in the chain or events. In one case the sectioning tech pulled the wrong block from the file, labeled the slides with that block number and sent it to the IHC group. An IHC tech took the slide and noticed the number was wrong but ASSUMED the sectioning tech had made an error, so "corrected" the number to match the request on her log. The mistake was only caught by the pathologist who noticed the IHC slide did not match the H&E slide from the proper block. I tell my techs: Trust your instincts. If something seems wrong - for whatever reason - STOP!!! Investigate the issue. You will save time, effort, and maybe lives. I also tell my techs not no pathologist will remember how fast you were, just how many mistakes you made. Tim Morken +++++++++++++++++++++++++++++++++++++++++++ Slide Labeling Procedure at the Microtome Slide labeling at the cutting station is the most important part of the process used to identify slides. A mistake at this point will cause a mix-up in slides or cases and could lead to improper diagnosis and treatment for patients. Because this is such an important step the following procedure must be followed exactly. Deviation may cause mislabeling. Deviation from the procedure may be cause for reprimand and termination. Important points: 1. Label slides for one block at a time. 2. Do not pre-label slides before cutting sections from a block, or for any other blocks. 3. Work with one block at a time as much as possible. If the block must be re-soaked before completing the slide count then the block must be isolated from the other blocks to prevent a mix-up. 4. The stainer tech will compare stained slides to the blocks to ensure they are correct. Sectioning Procedure 1) Pick up the next page of the processing log and the blocks that are on that page. 2) Verify that the blocks picked up are on the processing log. a. Put the blocks in order according to the processing log. b. Put a check mark ????????? next to the block line on the log to indicate the block is present in the group. c. Note any problems with the blocks 1. Put an ???X??? mark next to any problem blocks and note the problem. a. Add-ons b. Missing c. Reprocessing needed d. Re-embedding and reason e. other 3) Face-trim the blocks and put them on ice (if necessary) in order as shown on the processing log. 4) Pick up the first block on the log. a. Do not pre-label slides for this or any other block 5) Place the block in the microtome and cut sections according to a. requests on the processing log and established sectioning requirements b. ???Tissue Sectioning Standards??? of the Sectioning section of the Histology Laboratory manual 6) Pick up the proper number of sections on the appropriate slides. 7) Label the slides a. Write the Accession number and Part letter/number of the block on the microtome. b. Label slides with your initials (legibly) c. Label initial slide in the group with the cassette color and page number (this slide is first in the rack and faces forward for the Stainer person to see) d. NOTE: If the block must be re-soaked to complete the slide count, then isolate the block on the ice tray by putting it at the back of the tray facing backwards. This will indicate that some slides have been cut previously. 8) Place the slides for H&E in the H&E staining rack a. Write the color and page number of the processing log on the first slide of the rack. b. Place slides in the rack beginning at the front of the rack and working backward. c. Place designated ???unstained??? slides in separate rack(s) for use later (IPOX, Specials, etc). d. Discard any unused slides with sections on them. 9) Take the block from the microtome and put it in the cardboard filing box. 10) Clean the surface of the water with a Kimwipe or paper towel. Be sure there are no tissue fragments floating on the water bath. 11) Cross off that case on the processing log with a diagonal line through the entire case. 12) When the H&E slide rack is filled take it to the 60C oven a. Hand-write a label with the cassette color and page# as well as the time the slides will come out of the oven (10 min from current time). b. Place the label on the front end of the slide rack (end with first slide). c. Put the slides in the oven with the label facing forward. 13) When all the blocks have been cut, write your initials (legibly) on the bottom of the log and note the date/time. a. Compare Blocks and slides i. Give blocks to the Stainer area tech ii. When staining is completed the Stainer Tech compares the stained slides against the blocks and the process log. iii. If stained slides and blocks match, write ???Blocks/Slides match???, your initials and time at the bottom of the process log. iv. If slide(s) and block(s) do not match, determine the problem and correct it. v. Note the problem and correction on the process log along with your initials and time. b. Deliver the slides back to the Stainer Tech. 14) Place the cut blocks on the filing table. 15) Fill out a workload tally sheet for the day. -----Original Message----- From: Lynette Pavelich [mailto:LPaveli1@hurleymc.com] Sent: Monday, December 17, 2012 9:12 AM To: Sheila Haas; Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basis for Quality Work in a Histotech Would also love to hear! $$ for bar coding too far away! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, December 17, 2012 11:57 AM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech Tim, I'd be interested in more information in your labeling at the microtome that has all but eliminated errors. Would you share? Thank you, Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, December 17, 2012 11:30 AM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ??? counsel the HT after a mistake ??? retrain them ??? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren?? J. From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 109, Issue 19 ***************************************** This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From BGapinski <@t> pathgroup.com Mon Dec 17 12:41:41 2012 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Mon Dec 17 12:41:59 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech Message-ID: I agree with most everything said here. I'd like to add that I ask my staff to visualize the patient getting "accurate results in a timely fashion". In that order of importance. We really work for the patient, not the Pathologist or the company. If I don't tell them the good stuff, I can never address the bad. Another mistake is using fear to motivate. In other words, I've messed up 2 times this month one more and I'm out of a job. There has to be little risk for mistakes. And an environment of working together to change should help people with Histological pride. Here's how I do it. I call a meeting. I describe the problem, and give the person who did it, an "out". An out is an admission by me that the system needs tweaking. Blame is not going to get us the information we need. I say we, because my job is to exclude the ridiculous ideas, and choose the first one to try. It is a mistake for me to assume I have the answer. They do, the people doing the work. Now I observe and LISTEN. And as a group we agree on how to move forward. Sure they'll suggest stupid things, and I brush them aside. But if they make the rule, they will most likely follow it. Yes, you're right this is very Pollyanna of me and I know there are people we just can not reach. Problem people need extra prodding sometimes. But there comes a point when we just have to let them go. Documentation will help you move them on to another job that better suits their skill-set. Good reading is the American Samurai by William Lareau Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From joelleweaver <@t> hotmail.com Mon Dec 17 13:29:26 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Dec 17 13:29:34 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: References: Message-ID: Yes! I hate working in those "shame and blame" cultures, as does probably everyone! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Sharon.Genest@saskatoonhealthregion.ca > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Dec 2012 12:55:41 +0000 > Subject: [Histonet] Re: Basis for Quality Work in a Histotech > > My first recommendation would be to look at your process is there any way that you can error proof them? Make it more difficult to make the errors.When a lot of errors are occuring sometimes it is often due to how we do the job and not who does it. > > > Sharon Genest > Anatomic Pathology > Process Improvement > Saskatoon Health Region > 306-655-8242 > sharon.genest@saskatoonhealthregion.ca > his e-mail message may contain confidential and/or privileged information. It is intended only for the > addressee. Any unauthorized disclosure is strictly prohibited. If you are not a named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received > this e-mail by mistake and delete this e-mail from your system. E-mail transmissions cannot be guaranteed to be > secure or error free as information could be intercepted, corrupted, destroyed, arrive late or incomplete, or > contain viruses. The sender therefore does not accept any liability for errors or omissions in the contents of this > message or any damages that arise as a result of e-mail transmissions. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Mon Dec 17 13:46:12 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Dec 17 13:46:19 2012 Subject: [Histonet] Maximum bone sample size for methyl methacrylate embedding In-Reply-To: References: Message-ID: Orla, Based upon my personal and professional experience, the sample size of undecalcified bone which can be properly processed into methyl methacrylate for sectioning and staining for Goldner's trichrome is completely dependent upon your microtomy capabilities. For example, if you only have access to a motorized rotary microtome, you are limited to a specimen size that will generally fit onto a 25mm x 75mm microscope slide. In reality, you are looking at a specimen that will be sectioned thin @ approximately 4-6 microns and have a polymerized resin block size no greater than 15mm in width and 20-25mm in cutting length. This means that your specimen needs to fit within these dimensions and at least leave 0.5 - 1.0mm in plastic surrounding the tissue. Again, this is if you are limited by having only a motorized rotary microtome like a Leica RM2255 or RM2265. If you have something like an EXAKT cutting and grinding system for use with cutting thick sections of metallic medical device implants and then polishing sections down to 25-35 microns, you will likely use plastic slides that have a size limitation of 50mm x 100mm. This then means that your resin block will need to be contained within these dimensions of the slide, but without worry to a 0.5mm - 1.0mm plastic resin layer surrounding the tissue because you are cutting thick and grinding/polishing down to a desired final thickness. On the other hand, if you have access to a motorized sledge microtome like a Rechert-Jung Polycut S, E or a Leica SM 2500 for cutting thin 4-6 micron sections, you are only limited by the size of glass microscope slide you can purchase and of course the cutting width of the knife which is 200mm. However, most of my routine work with this piece of equipment is with a resin block limitation of 70mm x 90mm. Please keep in mind that what I am trying to say here is that you are mostly limited by the sectioning capabilities of the equipment that you have access to for microtomy. However, I will caution you that there is also a limitation in size based upon the mastery of the acrylic resin polymerization process. As you go up in size, you increase the difficulty in the ability to manage and control the exothermic polymerization of the specimen and block. Processing and polymerization of larger specimens is absolutely something that can be accomplished without issue, but only by experience that is learned over time through repetition and patience or by training from another that has mastered the technique, something that I have done for others in the past. I hope you understand that working with MMA is not simply something where one can provide you with a protocol and then you can easily expect the same results from one person to another. While I use the same general acrylic resin protocol for every type of bone, I frequently have to adjust the processing and infiltration times to compensate for the size and density of the specimen. I also have to then change how I manage or control the exothermic reaction. This is done by controlling the rate of polymerization, proportionally to the size of the tissue and specimen block, using temperature controls like adjusting room temperature, using a water bath, etc. Now to answer your question, the size you have defined as 2 cm x 1 cm is completely adequate and fairly routine to accomplish. If you message me back privately, I will provide you with a protocol to try. Additionally, if you are doing this for the first time and would like some initial help just to get you past this project until you can practice on your own, I can also offer my services to complete this project for you on contract. Of course, as I stated earlier, I can also train you on how to accomplish this technique as it is also something that I have done in the past for several labs both domestic and internationally. Best Regards, Jack Jack L RatliffRatliff Histology Consultants, LLC317-281-1975 > Date: Thu, 13 Dec 2012 17:44:04 +0000 > From: o.m.gallagher@sheffield.ac.uk > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Maximum bone sample size for methyl methacrylate embedding > > Dear Histonetters, > > Would anyone advise on the maximum size sample of undecalcified bone which > could be properly processed into methyl methacrylate for sectioning and > staining for Goldner's trichrome? Would anyone have a protocol for > processing large bone samples (possibly 2 x1cm) into MMA as most of the > protocols I've seen are based on Bordier trephine 5mm iliac crest biopsies. > > Thank you, > Orla > > -- > ************************** > Ms. Orla Gallagher > Bone Analysis Laboratory > Mellanby Centre for Bone Research > D Floor Medical School > University of Sheffield > Beech Hill Road > Sheffield > S10 2RX > > Website: http://mellanbycentre.dept.shef.ac.uk > > Tel: 00353114-2713337 (office) > 00353114-2713174 (lab) > E-Mail: o.m.gallagher@sheffield.ac.uk > > > *STOP*: Do you really need to print this e-mail? > > *BE GREEN:* Keep it on the screen. > > > *Times Higher Education University of the Year* > > > > Data protection and confidentiality: > The information contained in this message or any appended documents may be > privileged and confidential and is intended for the exclusive use of the > addressee(s). If you are not the addressee, any disclosure, reproduction, > distributions, other dissemination or use of this message is strictly > prohibited and may be unlawful. If you receive this correspondence in error > please contact the sender immediately and permanently delete/destroy what > you have received. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Dec 17 13:36:31 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Dec 17 14:36:34 2012 Subject: [Histonet] Stark Law Message-ID: Is anyone familiar with the Stark Law or can recommend a good resource? The lab is located in California. Thanks, Jennifer From CIngles <@t> uwhealth.org Mon Dec 17 14:46:10 2012 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Dec 17 14:46:16 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <761E2B5697F795489C8710BCC72141FF02FE6C@ex07.net.ucsf.edu> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu>, <1355763459.39171.YahooMailNeo@web122002.mail.ne1.yahoo.com> <89F4666A496DC949A819ECC40E11C867BF56D279@EXCHANGEMB1.hmc.hurleymc.com>, <761E2B5697F795489C8710BCC72141FF02FE6C@ex07.net.ucsf.edu> Message-ID: You're right Tim. I don't know how much time I have saved by listening to my inner voice and doublechecking things when the case number and name on a recut/stain request. One of our docs is famous for putting down wrong case #'s. Even when everything looks OK, sometimes the docs accidentally mark the wrong stain as our sheet is in a grid and they are mentally in the wrong column. I even get suspicious if they order an unusual stain that is sitting right next to a more routine on the list. I call it my "Histo sense" :) 5 minutes of double-checking is much more efficient than having to recut and restain again. Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Monday, December 17, 2012 11:52 AM To: Lynette Pavelich; Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basis for Quality Work in a Histotech Here is our slide labeling procedure. It is simple, but we insist it be followed. This will be modified once barcoding is instituted to use scanning ID of slides vs block. We also instituted block ID beads at the embedding center. That has helped tremendously to identify people that may need embedding retraining. One other thing I should mention. People in the lab should be told to trust their inner voice. We had several labeling errors that on investigation were suspected or ignored by a tech in the chain or events. In one case the sectioning tech pulled the wrong block from the file, labeled the slides with that block number and sent it to the IHC group. An IHC tech took the slide and noticed the number was wrong but ASSUMED the sectioning tech had made an error, so "corrected" the number to match the request on her log. The mistake was only caught by the pathologist who noticed the IHC slide did not match the H&E slide from the proper block. I tell my techs: Trust your instincts. If something seems wrong - for whatever reason - STOP!!! Investigate the issue. You will save time, effort, and maybe lives. I also tell my techs not no pathologist will remember how fast you were, just how many mistakes you made. Tim Morken +++++++++++++++++++++++++++++++++++++++++++ Slide Labeling Procedure at the Microtome Slide labeling at the cutting station is the most important part of the process used to identify slides. A mistake at this point will cause a mix-up in slides or cases and could lead to improper diagnosis and treatment for patients. Because this is such an important step the following procedure must be followed exactly. Deviation may cause mislabeling. Deviation from the procedure may be cause for reprimand and termination. Important points: 1. Label slides for one block at a time. 2. Do not pre-label slides before cutting sections from a block, or for any other blocks. 3. Work with one block at a time as much as possible. If the block must be re-soaked before completing the slide count then the block must be isolated from the other blocks to prevent a mix-up. 4. The stainer tech will compare stained slides to the blocks to ensure they are correct. Sectioning Procedure 1) Pick up the next page of the processing log and the blocks that are on that page. 2) Verify that the blocks picked up are on the processing log. a. Put the blocks in order according to the processing log. b. Put a check mark ??? next to the block line on the log to indicate the block is present in the group. c. Note any problems with the blocks 1. Put an ?X? mark next to any problem blocks and note the problem. a. Add-ons b. Missing c. Reprocessing needed d. Re-embedding and reason e. other 3) Face-trim the blocks and put them on ice (if necessary) in order as shown on the processing log. 4) Pick up the first block on the log. a. Do not pre-label slides for this or any other block 5) Place the block in the microtome and cut sections according to a. requests on the processing log and established sectioning requirements b. ?Tissue Sectioning Standards? of the Sectioning section of the Histology Laboratory manual 6) Pick up the proper number of sections on the appropriate slides. 7) Label the slides a. Write the Accession number and Part letter/number of the block on the microtome. b. Label slides with your initials (legibly) c. Label initial slide in the group with the cassette color and page number (this slide is first in the rack and faces forward for the Stainer person to see) d. NOTE: If the block must be re-soaked to complete the slide count, then isolate the block on the ice tray by putting it at the back of the tray facing backwards. This will indicate that some slides have been cut previously. 8) Place the slides for H&E in the H&E staining rack a. Write the color and page number of the processing log on the first slide of the rack. b. Place slides in the rack beginning at the front of the rack and working backward. c. Place designated ?unstained? slides in separate rack(s) for use later (IPOX, Specials, etc). d. Discard any unused slides with sections on them. 9) Take the block from the microtome and put it in the cardboard filing box. 10) Clean the surface of the water with a Kimwipe or paper towel. Be sure there are no tissue fragments floating on the water bath. 11) Cross off that case on the processing log with a diagonal line through the entire case. 12) When the H&E slide rack is filled take it to the 60C oven a. Hand-write a label with the cassette color and page# as well as the time the slides will come out of the oven (10 min from current time). b. Place the label on the front end of the slide rack (end with first slide). c. Put the slides in the oven with the label facing forward. 13) When all the blocks have been cut, write your initials (legibly) on the bottom of the log and note the date/time. a. Compare Blocks and slides i. Give blocks to the Stainer area tech ii. When staining is completed the Stainer Tech compares the stained slides against the blocks and the process log. iii. If stained slides and blocks match, write ?Blocks/Slides match?, your initials and time at the bottom of the process log. iv. If slide(s) and block(s) do not match, determine the problem and correct it. v. Note the problem and correction on the process log along with your initials and time. b. Deliver the slides back to the Stainer Tech. 14) Place the cut blocks on the filing table. 15) Fill out a workload tally sheet for the day. -----Original Message----- From: Lynette Pavelich [mailto:LPaveli1@hurleymc.com] Sent: Monday, December 17, 2012 9:12 AM To: Sheila Haas; Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basis for Quality Work in a Histotech Would also love to hear! $$ for bar coding too far away! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Monday, December 17, 2012 11:57 AM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech Tim, I'd be interested in more information in your labeling at the microtome that has all but eliminated errors. Would you share? Thank you, Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, December 17, 2012 11:30 AM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Travis, Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. All these methods need to be investigated. Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). Good luck with it! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, December 15, 2012 8:38 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J. From: Travis Troyer To: histonet@lists.utsouthwestern.edu Sent: Friday, December 14, 2012 5:34 PM Subject: [Histonet] Basis for Quality Work in a Histotech This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 17 15:09:13 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Dec 17 15:09:35 2012 Subject: Mondays fun fact RE: [Histonet] Maximum bone sample size for methyl methacrylate embedding Message-ID: <761E2B5697F795489C8710BCC72141FF02FFFF@ex07.net.ucsf.edu> Off topic but interesting anyway concerning acrylics. Just a fun fact since we use these chemicals.... Jack says below: " I will caution you that there is also a limitation in size based upon the mastery of the acrylic resin polymerization process. As you go up in size, you increase the difficulty in the ability to manage and control the exothermic polymerization of the specimen and block. " That is a huge problem in acrylics and while large acrylic structures are made now, they were mostly failures before an ARTIST (ie, a non-chemist) got involved in acrylic sculptures. I read an article years ago about an artist who figured out how to make massive acrylic sculptures. Chemists at Dupont thought it was impossible to do. The artist figured it out. He said one day it was like everything simply came together in his mind and the chemical process was evident. He went on to make acrylic bathyspheres for the Navy and Oil industry and undersea researchers - still the only person to have figured how to do it. BTW, he uses an autoclave to do the curing. A HUGE autoclave. Link below, click on Apolymon and Bathysphere http://www.brucebeasley.com/home.htm >From Wikipedia. In 1968, Bruce Beasley began investigating the use of transparency as a sculptural medium. He was successful in creating small transparent sculptures in cast acrylic but experts at Dupont and Rohm & Hass were convinced that it was impossible to do castings as large as Beasley envisioned. That year, the State of California invited Beasley to participate in a competition for a monumental sculpture for the state. At first, the jury was unaware that Beasley was experimenting with transparency as a sculptural medium and invited him based on his work in cast metal. Beasley was determined to pursue transparency and proposed a monumental cast acrylic sculpture. Upon seeing Beasley's proposal, they questioned the sculptor about its viability. He convinced them that creating what he envisioned was no problem but privately knew that he would have to invent a new process, which he did.[4] His proposal for Apolymon, a transparent sculpture in cast acrylic won and he installed the piece in Sacramento in 1970. 1970s Fascinated by the esthetics of transparency, Beasley worked in cast acrylic for the next ten years. In 1974, members of the undersea research community approached Beasley to see if he could adapt his technique to cast transparent bathyspheres for undersea exploration. He succeeded in creating the bathyspheres for Johnson Sea Link submersibles for Harbor Branch Oceanographic Institute. It was these submersibles that were deployed to locate the crew compartment on the bottom of the ocean after the Space Shuttle Challenger disintegrated upon liftoff in 1986. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Monday, December 17, 2012 11:46 AM To: Orla Gallagher; Histonet Cc: ratliffhistology@me.com; Jack Ratliff Subject: RE: [Histonet] Maximum bone sample size for methyl methacrylate embedding Orla, Based upon my personal and professional experience, the sample size of undecalcified bone which can be properly processed into methyl methacrylate for sectioning and staining for Goldner's trichrome is completely dependent upon your microtomy capabilities. For example, if you only have access to a motorized rotary microtome, you are limited to a specimen size that will generally fit onto a 25mm x 75mm microscope slide. In reality, you are looking at a specimen that will be sectioned thin @ approximately 4-6 microns and have a polymerized resin block size no greater than 15mm in width and 20-25mm in cutting length. This means that your specimen needs to fit within these dimensions and at least leave 0.5 - 1.0mm in plastic surrounding the tissue. Again, this is if you are limited by having only a motorized rotary microtome like a Leica RM2255 or RM2265. If you have something like an EXAKT cutting and grinding system for use with cutting thick sections of metallic medical device implants and then polishing sections down to 25-35 microns, you will likely use plastic slides that have a size limitation of 50mm x 100mm. This then means that your resin block will need to be contained within these dimensions of the slide, but without worry to a 0.5mm - 1.0mm plastic resin layer surrounding the tissue because you are cutting thick and grinding/polishing down to a desired final thickness. On the other hand, if you have access to a motorized sledge microtome like a Rechert-Jung Polycut S, E or a Leica SM 2500 for cutting thin 4-6 micron sections, you are only limited by the size of glass microscope slide you can purchase and of course the cutting width of the knife which is 200mm. However, most of my routine work with this piece of equipment is with a resin block limitation of 70mm x 90mm. Please keep in mind that what I am trying to say here is that you are mostly limited by the sectioning capabilities of the equipment that you have access to for microtomy. However, I will caution you that there is also a limitation in size based upon the mastery of the acrylic resin polymerization process. As you go up in size, you increase the difficulty in the ability to manage and control the exothermic polymerization of the specimen and block. Processing and polymerization of larger specimens is absolutely something that can be accomplished without issue, but only by experience that is learned over time through repetition and patience or by training from another that has mastered the technique, something that I have done for others in the past. I hope you understand that working with MMA is not simply something where one can provide you with a protocol and then you can easily expect the same results from one person to another. While I use the same general acrylic resin protocol for every type of bone, I frequently have to adjust the processing and infiltration times to compensate for the size and density of the specimen. I also have to then change how I manage or control the exothermic reaction. This is done by controlling the rate of polymerization, proportionally to the size of the tissue and specimen block, using temperature controls like adjusting room temperature, using a water bath, etc. Now to answer your question, the size you have defined as 2 cm x 1 cm is completely adequate and fairly routine to accomplish. If you message me back privately, I will provide you with a protocol to try. Additionally, if you are doing this for the first time and would like some initial help just to get you past this project until you can practice on your own, I can also offer my services to complete this project for you on contract. Of course, as I stated earlier, I can also train you on how to accomplish this technique as it is also something that I have done in the past for several labs both domestic and internationally. Best Regards, Jack Jack L RatliffRatliff Histology Consultants, LLC317-281-1975 > Date: Thu, 13 Dec 2012 17:44:04 +0000 > From: o.m.gallagher@sheffield.ac.uk > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Maximum bone sample size for methyl methacrylate embedding > > Dear Histonetters, > > Would anyone advise on the maximum size sample of undecalcified bone > which could be properly processed into methyl methacrylate for > sectioning and staining for Goldner's trichrome? Would anyone have a > protocol for processing large bone samples (possibly 2 x1cm) into MMA > as most of the protocols I've seen are based on Bordier trephine 5mm iliac crest biopsies. > > Thank you, > Orla > > -- > ************************** > Ms. Orla Gallagher > Bone Analysis Laboratory > Mellanby Centre for Bone Research > D Floor Medical School > University of Sheffield > Beech Hill Road > Sheffield > S10 2RX > > Website: http://mellanbycentre.dept.shef.ac.uk > > Tel: 00353114-2713337 (office) > 00353114-2713174 (lab) > E-Mail: o.m.gallagher@sheffield.ac.uk > > > *STOP*: Do you really need to print this e-mail? > > *BE GREEN:* Keep it on the screen. > > > *Times Higher Education University of the Year* > > > > Data protection and confidentiality: > The information contained in this message or any appended documents > may be privileged and confidential and is intended for the exclusive > use of the addressee(s). If you are not the addressee, any disclosure, > reproduction, distributions, other dissemination or use of this > message is strictly prohibited and may be unlawful. If you receive > this correspondence in error please contact the sender immediately and > permanently delete/destroy what you have received. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon Dec 17 16:56:14 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Dec 17 16:56:21 2012 Subject: [Histonet] Stark Law In-Reply-To: References: Message-ID: http://starklaw.org/ The Stark law keeps physicians from referring work to themselves - the very simple version.. See the web site for details.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, December 17, 2012 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stark Law Is anyone familiar with the Stark Law or can recommend a good resource? The lab is located in California. Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From pathlocums <@t> gmail.com Mon Dec 17 18:12:53 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Dec 17 18:13:09 2012 Subject: [Histonet] Stark Law In-Reply-To: References: Message-ID: <574400547824390539@unknownmsgid> Google Jane Pine Wood - best Stark law attorney in the US. Sent from my iPhone On Dec 17, 2012, at 12:36 PM, Jennifer MacDonald wrote: > Is anyone familiar with the Stark Law or can recommend a good resource? > The lab is located in California. > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Mon Dec 17 19:58:17 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Mon Dec 17 19:58:20 2012 Subject: [Histonet] H&E with Mayers Message-ID: Hi Everyone:If anyone is using Mayers Haematoxylin progressively, can you please share your H&E protocol with me?Thanks:)Sheila From wilson6848 <@t> yahoo.com Mon Dec 17 20:17:37 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Mon Dec 17 20:17:40 2012 Subject: [Histonet] Disposable Blades and Handles Message-ID: <1355797057.75498.YahooMailNeo@web125403.mail.ne1.yahoo.com> ? ? ?Hi, ???? Please we are looking for the type of disposable blades and handles that we can use to cut frozen sections on our TBS CRYOSTAT. Any suggestios will be highy appreciated. ? Thanks, ? Wilson From mw <@t> personifysearch.com Tue Dec 18 07:51:57 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Dec 18 07:52:03 2012 Subject: [Histonet] New IHC Opportunity Message-ID: Good Morning Histonet, Our client has recently opened a new IHC opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in Histology and IHC has recently opened a new IHC opportunity in the Midwest Region to focus on driving IHC Reagent and Antibody sales. We are searching for a histology professional who has a strong background in IHC and antibodies that would be interested in working in a team sales environment. Though sales experience is a plus, this position does not require previous sales experience and this would be outstanding opportunity for someone looking to break into sales that has a strong histology background. The ideal location for the position would be in the greater Minneapolis area, however we will consider candidates in the region (MN, ND, SD, IA, NE, KS). The position offer s strong package including a base salary, competitive commission structure, car allowance, gas card, cell phone, laptop computer, and full benefits. If you or anyone you may know would be interested in the position, please contact me directly at mw@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From turkekul <@t> gmail.com Tue Dec 18 10:20:26 2012 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Tue Dec 18 10:21:45 2012 Subject: [Histonet] Bluing Message-ID: Dear Histonetters, I wonder what is the purpose of bluing the hematoxylin. Can bluing effect the clarity of the nuclear staining? Can one achieve different bluing results varying the composition, pH or time of the bluing reagent? Mes From wdesalvo.cac <@t> outlook.com Tue Dec 18 10:57:46 2012 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Dec 18 10:57:50 2012 Subject: [Histonet] Bluing In-Reply-To: References: Message-ID: Blueing will differentiate and can help crisp the stain. The pH does affect the rate and intensity of color change. You want a slightly alkaline solution to ?Blue? your slides. The blueing process will differentiate the normal purplish color of the acid dye, Hematoxylin (pH 2.6-2.9) by changing the color to a blue from the reddish purple. This process produces a better contrast with the usual red counter stains, Eosin and Phloxine, used in the H&E. Common blueing/differentiators I have used are; 0.1% Sodium Bicarbonate (1 g / 1000 ml distilled water), 0.2% Ammonia Water (2 ml / 1000 ml distilled water) and Saturated Lithium Carbonate (1.54 g / 100 ml distilled water). You can also use ?tap water?, but be careful about how the water is treated. Rinsing in tap or distilled water also provides a crisper stain because is rinses away the alum. If alum remains, the color will fade with time. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Tue, 18 Dec 2012 11:20:26 -0500 > From: turkekul@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bluing > > Dear Histonetters, > > > I wonder what is the purpose of bluing the hematoxylin. Can bluing effect > the clarity of the nuclear staining? Can one achieve different bluing > results varying the composition, pH or time of the bluing reagent? > > > Mes > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Tue Dec 18 11:36:04 2012 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Dec 18 11:36:12 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> Message-ID: <43A1727F-3B45-46B0-A5F8-4638A38440B9@gmail.com> Travis, I agree with everyone's valuable thoughts regarding your question. As a supervisor, it's imperative to communicate with people in such a way that they change themselves. With some people the act of "getting the task done," has such an urgent need that can lead them to become careless & aggressive, leaping before looking & even speaking without thinking first. In histology, we know it's more important to avoid making mistakes - to be certain every detail is accurate & in place. And, it's important to find the balance between the 2 intentions of "getting the task done" & "getting it done right!" It's also important to create & develop relationships with those you work with. The desire to contribute to others & be appreciated for what you do, is one of the most powerful motivational forces known. And yes, sometimes you get what you give! Giving appreciation & getting along with others go hand-in-hand - & as a supervisor it's another balancing act. Also when people have the same priorities, a misunderstanding or conflict is highly UNLIKELY. All the best Maria Mejia San Francisco, CA On Dec 17, 2012, at 8:30 AM, Morken, Timothy wrote: > Travis, > > Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. > > The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). > > Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. > > For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). > > We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. > > All these methods need to be investigated. > > Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). > > > Good luck with it! > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Saturday, December 15, 2012 8:38 AM > To: Travis Troyer; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Basis for Quality Work in a Histotech > > First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. > You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. > With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. > Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. > There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. > The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. > It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J. > > > From: Travis Troyer > To: histonet@lists.utsouthwestern.edu > Sent: Friday, December 14, 2012 5:34 PM > Subject: [Histonet] Basis for Quality Work in a Histotech > > This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. > > Thanks, > Travis Troyer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lange <@t> kennedykrieger.org Tue Dec 18 11:48:58 2012 From: lange <@t> kennedykrieger.org (Lange, Mollie) Date: Tue Dec 18 11:49:07 2012 Subject: [Histonet] help with myelin staining in poorly osmicated tissue Message-ID: Is there an alternative to toluidine blue stain for plastic embedded tissue? I have some valuable epoxy resin embedded rat spinal cord blocks that were not well osmicated. Toluidine Blue staining of the semi-thin sections is uneven and pale in the center of the sections. Is there another stain I could try that is not dependent on good osmium penetration? I fear that alcohol dehydration and propylene oxide clearing may have demyelinated the poorly osmicated portions of the block. In that case, no stain will work. But I'm not ready to give up yet. Thanks for any and all advice. I've been out of the lab for a while. Mollie Lange Project Manager International Center for Spinal Cord Injury Huo W. Moser Research Institute at Kennedy Krieger 707 N. Broadway Baltimore, MD 21205 443-923-9241 phone 443-923-9245 fax lange@kennedykrieger.org Disclaimer: The materials in this e-mail are private and may contain Protected Health Information. Please note that e-mail is not necessarily confidential or secure. Your use of e-mail constitutes your acknowledgment of these confidentiality and security limitations. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via telephone or return e-mail. From Norm.Burnham <@t> propath.com Tue Dec 18 12:28:23 2012 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Tue Dec 18 12:28:28 2012 Subject: [Histonet] Immediate Opening -- Histology Supervisor Message-ID: <82C7248978CB50469FD6BA68EBBEFE6709277244@exchange.propathlab.com> Immediate Opening for Supervisor of Histology ProPath, a progressive, CAP accredited, a high-volume, pathology practice in Dallas Texas needs an experienced team-orientated leader to supervise all day-to-day operations of the histology department. Duties include but are not limited to recruiting, hiring, training, and scheduling staff; supervising histology operation to ensure quality of slides and that department goals are met; maintaining preparedness for all accrediting/regulatory agency inspections and general troubleshooting. The ideal candidate will have a minimum of 5 years histology experience to include a minimum of 3 years in a leadership role. HT or HTL (ASCP) certification is required. Bachelors' degree in biological/physical science preferred. Strong knowledge of laboratory operations, pathology knowledge desired. Excellent verbal and written communication skills. Basic computer skills. Must be available to work nights. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, short and long term disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to: accessibility@propath.com ______________________________________________ Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 norm.burnham@propath.com 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell To learn more about ProPath, please visit www.propath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From Norm.Burnham <@t> propath.com Tue Dec 18 12:33:11 2012 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Tue Dec 18 12:33:17 2012 Subject: [Histonet] Immediate Opening for a Pathologists' Assistant Message-ID: <82C7248978CB50469FD6BA68EBBEFE670927724D@exchange.propathlab.com> IMMEDIATE OPENING FOR A PATHOLOGISTS' ASSISTANT ProPath, a high volume, privately owned pathology practice located in Dallas, Texas, is seeking a Pathologists' Assistant. In this position you will be responsible for accurate description of specimens, correct dissection for microscopic diagnosis and dictation of findings for pathologist's review. The ideal candidate will have a minimum of 3 years' experience. Prefer a degree from a NACCLS-accredited Pathologists' Assistant program. AAPA Fellowship or ASCP certification as a Pathologists' Assistant is required. The hours for this position are 6pm to 2:30am Monday - Friday. Benefits include Medical, Dental, Short and Long Term Disability Insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EOE For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 www.propath.com Accessibility Accommodations If you require an accommodation to navigate our careers site, please send your request to: accessibility@propath.com ______________________________________________ Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 norm.burnham@propath.com 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell To learn more about ProPath, please visit www.propath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From billodonnell <@t> catholichealth.net Tue Dec 18 13:37:59 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Dec 18 13:37:47 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <43A1727F-3B45-46B0-A5F8-4638A38440B9@gmail.com> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local><1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com><761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> <43A1727F-3B45-46B0-A5F8-4638A38440B9@gmail.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93958EFCD6@chimsx08.CHI.catholichealth.net> Maria - Well said.=20 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@li= sts.utsouthwestern.edu] On Behalf Of Maria Mejia Sent: Tuesday, December 18, 2012 11:36 AM To: Morken, Timothy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech Travis, I agree with everyone's valuable thoughts regarding your question. As a su= pervisor, it's imperative to communicate with people in such a way that the= y change themselves. With some people the act of "getting the task done," has such an urgent n= eed that can lead them to become careless & aggressive, leaping before look= ing & even speaking without thinking first. In histology, we know it's mor= e important to avoid making mistakes - to be certain every detail is accura= te & in place. And, it's important to find the balance between the 2 intent= ions of "getting the task done" & "getting it done right!" It's also important to create & develop relationships with those you work w= ith. The desire to contribute to others & be appreciated for what you do, = is one of the most powerful=20 motivational forces known. And yes, sometimes you get what you give! Giv= ing appreciation & getting along with others go hand-in-hand - & as a super= visor it's another balancing act. Also when people have the same priorities, a misunderstanding or conf= lict is highly UNLIKELY. All the best Maria Mejia San Francisco, CA On Dec 17, 2012, at 8:30 AM, Morken, Timothy wrote: > Travis, >=20 > Histology has a very complex workflow AND requires "artisan" level workma= nship to deliver a product. Those two together nearly guarantee mistakes, m= ostly minor, but sometimes literally life-threatening to patients. The goal= is to instill a sense of Best Quality in the techs. A large part of achiev= ing that attitude is to ensure the pathologists and administrators are behi= nd the techs 100% and ALLOW the techs to do Best Quality - ie, accept that = Best Quality will sometimes mean slower turnaround time. Does that aspect m= ean more people are needed? That's your call, but can be determined by work= load accounting. >=20 > The attitude should be that the SYSTEM makes the mistake, not the individ= ual. It is not likely a person makes a mistake on purpose, but instead is i= t some aspect of the system that allows them to make a mistake (though "sho= rtcuts" can be thought of as intentionally risking making mistakes "on purp= ose," the "purpose" being to save time or effort). >=20 > Workflows can be "engineered" to ensure some mistakes don't happen. Proto= cols must be followed to the letter by EVERYONE. No workarounds allowed (a = workaround is an indication that there is something wrong in the system - t= he employee feels the need to take shortcuts. Why? BTW, Bill Gates said th= e most important word in his vocabulary is "why." Why is something done the= way it is? Why does a mistake happen at a certain point? ). In failure ana= lysis a problem is approached by asking 5 levels of WHY? After asking WHY 5= times back down the workflow chain you usually find the root cause of a pr= oblem. If not, you keep asking why until the root cause is found. >=20 > For instance, we worked out a slide labeling protocol at the microtome th= at, if followed, will ensure the tech does not make labeling errors. All pa= rticipated in working this out and so have bought into the system. All new = employees are trained in that system. That will eventually be followed by b= arcoding, but that is a year away at least. But our protocol has nearly eli= minated labeling errors (we still get a few sneaking in here and there but = as we catch them we try to figure out how to engineer them away). >=20 > We also finally instituted the printing of cassettes directly from our LI= S rather than using a stand-alone printer or hand-writing. That has almost = totally eliminated cassette labeling errors - we used to have hundreds per = month, mainly by residents putting in cassettes that they did not enter in = our LIS, or making simple typo errors on a stand-alone cassette labeler, or= hand-written cassettes.=20 >=20 > All these methods need to be investigated.=20 >=20 > Rewards are also very helpful. We give out "Bear hugs" that are $5 gift c= ertificates to the campus store, cafeteria, various food vendors in the ins= titution, etc. it's a small reward, but people actually appreciate it. We a= lso have "Star Awards" of $50 gift cards for those times when someone does = something more beyond the usual. The receiver chooses the card they want fr= om about 2 dozen available (coffee shops, VISA, various stores, etc). >=20 >=20 > Good luck with it! >=20 >=20 > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies=20 > Department of Pathology UC San Francisco Medical Center >=20 >=20 >=20 >=20 >=20 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu=20 > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J=20 > Buesa > Sent: Saturday, December 15, 2012 8:38 AM > To: Travis Troyer; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Basis for Quality Work in a Histotech >=20 > First I want you to excuse me, but I do not think that you are really qua= lified to supervise 2 histotechs if you need to ask for such quality guidan= ce. > You end by bemoaning about "budget crunch" and because of that it seems t= o me that your 2 histotechs are not receiving a "decent" salary and, as eve= rybody knows, you are getting what you are paying for. > With 10 years of experience you should know that the first step for quali= ty of sections is quality of fixation and quality of processing. You have f= irst to manage that aspect. > Quality of sections comes after wards and there is no "standard" for mist= akes and for what you are describing it seems that mistakes are frequent. B= y the way, if the pathologists are not pleased, they will not it take on th= e histotechs, but on you as a supervisor unable to provide them the quality= they require. > There is no such thing as "instant reward" for a good quality job; the hi= stotech should not be treated as "dogs receiving a cookie after a trick per= formed" but there are 2 tools: you need to keep track of the mistakes =A1= =FA counsel the HT after a mistake =A1=FA retrain them =A1=FA keep a track = of mistakes and there are verbal and written counselings and an annual eval= uation, I am sure you know that. > The ideal limit of mistakes is "0" but there is some acceptable mistakes = limits, as long as they are few and far between. The pathologists are the o= nes who can tell you what they are willing to accept as mistakes limits. As= k them. > It seems that if your 2 HTs do not improve, you should start looking for = replacements, but they should be better paid, and if the mistakes continue = at a high rate, you should put your 10 years experience to work and start d= oing some bench work Ren=A8=A6 J.=20=20=20=20 >=20 >=20 > From: Travis Troyer > To: histonet@lists.utsouthwestern.edu > Sent: Friday, December 14, 2012 5:34 PM > Subject: [Histonet] Basis for Quality Work in a Histotech >=20 > This is a question for all of the lab supervisors. I am the supervisor o= f two histotechs. I am not doing techwork now, but have 10 years of experi= ence. The pathologists are getting more and more upset at the lack of qual= ity in the work and the mistakes that are happening. I was wondering if an= yone had some ideas on what sort of a goal to set up and how to reward/puni= sh for variations from that goal. For example, if the goal is three mista= kes for the month, what is the best way to reward them for making that goal= and what would be best if they had more mistakes in a given time frame. W= e are all feeling the budget crunch and the pathologists are trying to figu= re out a good solution. >=20 > Thanks, > Travis Troyer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the= named addressee(s) and contain confidential information. If you are not an= addressee, or responsible for delivering this email to an addressee, you h= ave received this email in error and are notified that reading, copying, or= disclosing this email is prohibited. If you received this email in error, = immediately reply to the sender and delete the message completely from your= computer system. From Janine.SimmsColon <@t> jmmc.com Tue Dec 18 13:45:42 2012 From: Janine.SimmsColon <@t> jmmc.com (SimmsColon, Janine) Date: Tue Dec 18 13:46:53 2012 Subject: [Histonet] Hematoxylin issue Message-ID: Good afternoon all, I have been addressed with an issue which I would like some assistance with. My pathologist recently reviewed some skin slides from 2007 and noticed the hematoxylin was completely gone and only the eosin remained. We use Gill's II hematoxylin, and glass cover slips with xylene substitute mountant. The concern is that since we must retain slides for 10 years and the stain has "washed out" after only 5 this could be a pretty big problem. I do not believe this particular lab has experienced this problem before but I have only worked here for less than one year. We stain in hematoxylin for 3 minutes, use commercially available clarifier and bluing solution, each for one minute as well as one minute tap water rinses in between and dip in 95% alcohol before and after a 1% alcoholic eosin y solution. I searched the histonet archives for this problem as well which is why I am mentioning the staining process but I was curious if anyone out there had any suggestions or advice to avoid this issue for future slides. Thank you in advance. Janine Simms Colon, CPhT(PTCB), HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. From Barry.R.Rittman <@t> uth.tmc.edu Tue Dec 18 14:10:37 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Dec 18 14:10:35 2012 Subject: [Histonet] RE: Hematoxylin issue In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDE25@UTHCMS1.uthouston.edu> Difficult to say without seeing but there could be many reasons for this. The hematoxylin could have either been removed by traces of acid before mountiung or if transferred to the mounting medium. Hematoxylin could have been further oxidized to a colorless oxyhematein. This can occur with some mounting media , exposiure to light etc. Too alkaline mountiung medioum unlikely or else you would have also lost the eosin. Not sure about xylene substitute. It is possible to retain hematoxylin - I have some slide stained in 1957 and they still have excellent hematoxylin and eosin staining but were mounted in Canada balsam in xylene. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SimmsColon, Janine [Janine.SimmsColon@jmmc.com] Sent: Tuesday, December 18, 2012 1:45 PM To: Histonet Subject: [Histonet] Hematoxylin issue Good afternoon all, I have been addressed with an issue which I would like some assistance with. My pathologist recently reviewed some skin slides from 2007 and noticed the hematoxylin was completely gone and only the eosin remained. We use Gill's II hematoxylin, and glass cover slips with xylene substitute mountant. The concern is that since we must retain slides for 10 years and the stain has "washed out" after only 5 this could be a pretty big problem. I do not believe this particular lab has experienced this problem before but I have only worked here for less than one year. We stain in hematoxylin for 3 minutes, use commercially available clarifier and bluing solution, each for one minute as well as one minute tap water rinses in between and dip in 95% alcohol before and after a 1% alcoholic eosin y solution. I searched the histonet archives for this problem as well which is why I am mentioning the staining process but I was curious if anyone out there had any suggestions or advice to avoid this issue for future slides. Thank you in advance. Janine Simms Colon, CPhT(PTCB), HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Tue Dec 18 14:22:49 2012 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Dec 18 14:22:53 2012 Subject: [Histonet] Hematoxylin issue In-Reply-To: References: Message-ID: I suggest you look at the reagents and protocol used 5 years ago and first determine if the same is used today. If you know all the reagents used, then start by check three specifics of the process: 1.) Removal of alum - make sure the slides are sufficiently rinsed. Suggested is 3-4 minutes to adequately remove the alum, you are using only 1 minute; 2.) Mounting Media - make sure the mounting media used had an antioxidant to prevent fading; 3.) Xylene - mixtures can cause fading, you made need to move to higher grade. This is only a starting point, having the exact protocol and reagents used then and compare them to now is critical. I would pull samples for each year forward from the found problem and check to see if you have fading. I do not have an easy answere to fix and it may be necessary to recut slides, from the retained blocks, if the archived case is requested for review. Most important is to find the cause, stop the problem and move forward. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Tue, 18 Dec 2012 14:45:42 -0500 > From: Janine.SimmsColon@jmmc.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hematoxylin issue > > Good afternoon all, > > > > I have been addressed with an issue which I would like some assistance > with. My pathologist recently reviewed some skin slides from 2007 and > noticed the hematoxylin was completely gone and only the eosin remained. > We use Gill's II hematoxylin, and glass cover slips with xylene > substitute mountant. The concern is that since we must retain slides for > 10 years and the stain has "washed out" after only 5 this could be a > pretty big problem. I do not believe this particular lab has experienced > this problem before but I have only worked here for less than one year. > We stain in hematoxylin for 3 minutes, use commercially available > clarifier and bluing solution, each for one minute as well as one minute > tap water rinses in between and dip in 95% alcohol before and after a 1% > alcoholic eosin y solution. I searched the histonet archives for this > problem as well which is why I am mentioning the staining process but I > was curious if anyone out there had any suggestions or advice to avoid > this issue for future slides. Thank you in advance. > > > > Janine Simms Colon, CPhT(PTCB), HT(ASCP) > > Histology/Pathology > > Johnson Memorial Hospital > > 201 Chestnut Hill Road > > Stafford Springs, CT 06076 > > Office: 860-684-8230 ext. 5197 > > janine.simmscolon@jmmc.com > > > > > The information contained in this message may be privileged and confidential and protected from disclosure. > If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If you have received this communication in error, please > notify us immediately by replying to the message and deleting it from your computer. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mkent <@t> dermpathlab.com Tue Dec 18 15:25:32 2012 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Tue Dec 18 15:26:39 2012 Subject: [Histonet] 88305TC starting to hit the fan... Message-ID: <27EB7FFB1A15F549B17F79B1A856C70BAD5310@dlcs-sbs1.DPLCS.intra> Fellow Histoneters, Please feel free to contact me if your lab is in trouble from these cuts. We may be able to help. Also- we are hiring a Histotech in the Troy Michigan area, if you know anyone interested. Best and Happy Holidays, Michael Kent, MS, PhD Translational Scientist Dermatopathology Laboratory of Central States Adjunct Assistant Professor of Dermatology Wright State University Boonshoft School of Medicine 7835 Paragon Rd. Dayton OH 45459 (937) 436-4152 www.dermpathlab.com Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. From akemiat3377 <@t> yahoo.com Tue Dec 18 17:08:48 2012 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Dec 18 17:08:52 2012 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <27EB7FFB1A15F549B17F79B1A856C70BAD5310@dlcs-sbs1.DPLCS.intra> References: <27EB7FFB1A15F549B17F79B1A856C70BAD5310@dlcs-sbs1.DPLCS.intra> Message-ID: <1355872128.49865.YahooMailNeo@web113812.mail.gq1.yahoo.com> Hello: You stated "88305TC starting to hit the fan..." Would you care to explain some of the details with us. I work in a private GI lab which contracts our pathology PC mortion to local pathologists.??One?of which is our medical director, as well as the medical director for a local community hospital.? They come to our facility to do the microscopic portion of their work. ? Regards, Akemi Akemi Allison, BS, HT(ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com ________________________________ From: Michael Kent To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 18, 2012 1:25 PM Subject: RE: [Histonet] 88305TC starting to hit the fan... Fellow Histoneters, Please feel free to contact me if your lab is in trouble from these cuts.? We may be able to help.? Also- we are hiring a Histotech in the Troy Michigan area, if you know anyone interested. Best and Happy Holidays, ? Michael Kent, MS, PhD Translational Scientist Dermatopathology Laboratory of Central States Adjunct Assistant Professor of Dermatology Wright State University Boonshoft School of Medicine 7835 Paragon Rd. Dayton OH 45459 (937) 436-4152 http://www.dermpathlab.com/ Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Dec 19 02:54:18 2012 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Wed Dec 19 02:54:28 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gODgzMDVUQyBzdGFydGluZyB0byBoaXQgdGhlIGZhbi4u?= =?utf-8?B?Lg==?= Message-ID: Do you have a job description, shift etc. For your opening(s)? Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Michael Kent" To: Subject: [Histonet] 88305TC starting to hit the fan... Date: Tue, Dec 18, 2012 3:25 pm Fellow Histoneters, Please feel free to contact me if your lab is in trouble from these cuts. We may be able to help. Also- we are hiring a Histotech in the Troy Michigan area, if you know anyone interested. Best and Happy Holidays, Michael Kent, MS, PhD Translational Scientist Dermatopathology Laboratory of Central States Adjunct Assistant Professor of Dermatology Wright State University Boonshoft School of Medicine 7835 Paragon Rd. Dayton OH 45459 (937) 436-4152 www.dermpathlab.com Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Dec 19 08:47:35 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Dec 19 08:47:50 2012 Subject: [Histonet] thyroid processing Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4301FF92B7B1A3@HPEMX3.HealthPartners.int> Does anyone have some good tips to processing thyroid tissue from thyroidectomy specimens? There seems to be a fine line between underprocessing and overprocessing where the tissue becomes brittle. We currently process them on an overnite run where the tissue is in all reagents for 45 minutes. We have also tried a shorter run on the Peloris that takes about 6 hours. Would like to know what others are doing for more optimal tissue processing!! Thanks so much everyone! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Pathrm35 <@t> comcast.net Wed Dec 19 11:45:34 2012 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Wed Dec 19 11:45:42 2012 Subject: [Histonet] 2 CAP questions In-Reply-To: <161372579.1635944.1355939041313.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <594004571.1636028.1355939134791.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Fellow techs, 1) what type of a back up power source are you using for your tissue processors, refridgerators and freezors? 2) What do you do about calibration for NIST thermometers and non certified thermometers? Thanks, Ron From Pathrm35 <@t> comcast.net Wed Dec 19 11:55:32 2012 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Wed Dec 19 11:55:36 2012 Subject: [Histonet] 2 CAP questions In-Reply-To: <594004571.1636028.1355939134791.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <1304908660.1636459.1355939732832.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Please excuse my typos. ----- Original Message ----- From: Pathrm35@comcast.net To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 19, 2012 12:45:34 PM Subject: [Histonet] 2 CAP questions Fellow techs, 1) what type of a back up power source are you using for your tissue processors, refridgerators and freezors? 2) What do you do about calibration for NIST thermometers and non certified thermometers? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Wed Dec 19 13:50:12 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Dec 19 13:50:16 2012 Subject: [Histonet] Histotechnician Salary Message-ID: <50D21A74.5070009@mclean.harvard.edu> Hi Everybody: I manage a neuropathology laboratory at a brain tissue bank. We are going to be requesting funds from the NIH for a full-time histotechnician to work with me. We would like someone who is HT or HTL certified, has at least 5 years of experience, preferably in neuropathology, and can work independently. However, I have no idea what the salary would be for this kind of position. Could people let me know how much such a position-or a comparable one in a surgical histology lab-pays? Thank you, Tim Wheelock Assistant Director, Neuropathology Laboratory Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA. From twheelock <@t> mclean.harvard.edu Wed Dec 19 14:01:28 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Dec 19 14:01:33 2012 Subject: [Histonet] Histotechnician Salary 2 Message-ID: <50D21D18.2020503@mclean.harvard.edu> Hi again all: I should have mentioned that the position calls for doing classical neuropathology stains, but not IHC. We have our immunostains done by another lab. Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 19 14:14:05 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 19 14:14:23 2012 Subject: [Histonet] Histotechnician Salary 2 In-Reply-To: <50D21D18.2020503@mclean.harvard.edu> References: <50D21D18.2020503@mclean.harvard.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF03033C@ex07.net.ucsf.edu> Tim, it depends on the going rate in your area. What does your hospital pay a general histotech? How about other hospitals in the area. I'll bet your personnel dept has that data. In California it can range from $15 to $35 per hour for histotechs with 5 years experience. The lower is in the rural areas and the higher in the San Francisco or LA areas. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, December 19, 2012 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnician Salary 2 Hi again all: I should have mentioned that the position calls for doing classical neuropathology stains, but not IHC. We have our immunostains done by another lab. Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Dec 19 14:17:30 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Dec 19 14:17:35 2012 Subject: [Histonet] Basis for Quality Work in a Histotech In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93958EFCD6@chimsx08.CHI.catholichealth.net> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local><1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com><761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> <43A1727F-3B45-46B0-A5F8-4638A38440B9@gmail.com> <4940DF6D1C5FDF48931B6966AAEF93958EFCD6@chimsx08.CHI.catholichealth.net> Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Id like to add my 2cents to the measuring "Quality" topic. I'll make it short. ? You should have a "Quality Managment" program. It's vital to track errors, trypes of errors, frequency and who etc. This is NOT a tool for blame as we are all adults or we should be. It is however a tool for tracking trends, making improvments and yes if you did see someone making a mistake often, you would have the data to educate particular personel. ? There are QM tools out there from various organizations. And yes, there are standards of deviations such as the TAT for frozens. There are standards for other thangs as well. Set Standards of excellence?with your Pathologist. Make goals. Track them. Follow improvment. ? So try a google on QM and see if that helps answer any of the original questions. ? BTW... Merry Christmas Histonettters. :) ? Kim :D ________________________________ From: "O'Donnell, Bill" To: Maria Mejia ; "Morken, Timothy" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 18, 2012 2:37 PM Subject: RE: [Histonet] Basis for Quality Work in a Histotech Maria - Well said. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Mejia Sent: Tuesday, December 18, 2012 11:36 AM To: Morken, Timothy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basis for Quality Work in a Histotech Travis, I agree with everyone's valuable thoughts regarding your question.? As a supervisor, it's imperative to communicate with people in such a way that they change themselves. With some people the act of? "getting the task done,"? has such an urgent need that can lead them to become careless & aggressive, leaping before looking & even speaking without thinking first.? In histology, we know it's more important to avoid making mistakes - to be certain every detail is accurate & in place. And, it's important to find the balance between the 2 intentions of "getting the task done" & "getting it done right!" It's also important to create & develop relationships with those you work with.? The desire to contribute to others & be appreciated for what you do, is one of the most powerful motivational forces known.? And yes, sometimes you get what you give!? Giving appreciation & getting along with others go hand-in-hand - & as a supervisor it's another balancing act.? Also when people have the same priorities, a misunderstanding or conflict is highly UNLIKELY. All the best Maria Mejia San Francisco, CA On Dec 17, 2012, at 8:30 AM, Morken, Timothy wrote: > Travis, > > Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. > > The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). > > Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why?? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. > > For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). > > We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. > > All these methods need to be investigated. > > Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). > > > Good luck with it! > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Saturday, December 15, 2012 8:38 AM > To: Travis Troyer; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Basis for Quality Work in a Histotech > > First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. > You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. > With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. > Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. > There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. > The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. > It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J.? ? > > > From: Travis Troyer > To: histonet@lists.utsouthwestern.edu > Sent: Friday, December 14, 2012 5:34 PM > Subject: [Histonet] Basis for Quality Work in a Histotech > > This is a question for all of the lab supervisors.? I am the supervisor of two histotechs.? I am not doing techwork now, but have 10 years of experience.? The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening.? I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal.? For example,? if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame.? We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. > > Thanks, > Travis Troyer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Wed Dec 19 14:53:37 2012 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed Dec 19 14:53:43 2012 Subject: [Histonet] Histotechnician Salary 2 In-Reply-To: <761E2B5697F795489C8710BCC72141FF03033C@ex07.net.ucsf.edu> References: <50D21D18.2020503@mclean.harvard.edu>, <761E2B5697F795489C8710BCC72141FF03033C@ex07.net.ucsf.edu> Message-ID: I agree w/ Tim that the salary range is very wide and depending upon the geographical area. ASCP and Advance magazine publish salary surveys by area. I suggest you factor in the cost of benefits, since you are asking for funding from a government entity. Benefits can range from 35%- 40% of salary. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: Timothy.Morken@ucsfmedctr.org > To: twheelock@mclean.harvard.edu; histonet@lists.utsouthwestern.edu > Date: Wed, 19 Dec 2012 20:14:05 +0000 > Subject: RE: [Histonet] Histotechnician Salary 2 > CC: > > Tim, it depends on the going rate in your area. What does your hospital pay a general histotech? How about other hospitals in the area. I'll bet your personnel dept has that data. > > In California it can range from $15 to $35 per hour for histotechs with 5 years experience. The lower is in the rural areas and the higher in the San Francisco or LA areas. > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock > Sent: Wednesday, December 19, 2012 12:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histotechnician Salary 2 > > Hi again all: > > I should have mentioned that the position calls for doing classical neuropathology stains, but not IHC. > We have our immunostains done by another lab. > > Thanks, > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Wed Dec 19 15:30:44 2012 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Wed Dec 19 15:30:51 2012 Subject: [Histonet] CD31 clone WM59 Message-ID: Does anyone know if this clone WM59 crosses to mouse CD31? Andrea From Rcartun <@t> harthosp.org Wed Dec 19 16:07:11 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 19 16:07:18 2012 Subject: [Histonet] PAI-1 Message-ID: <50D1F43F.7400.0077.1@harthosp.org> Does anyone do IHC for PAI-1? Thank you. Happy Holidays to all and best wishes for 2013. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax From tony.henwood <@t> health.nsw.gov.au Wed Dec 19 17:18:41 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Dec 19 17:18:58 2012 Subject: [Histonet] RE: thyroid processing In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4301FF92B7B1A3@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4301FF92B7B1A3@HPEMX3.HealthPartners.int> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D20E2D1@xmdb04.nch.kids> My recommendation is to: 1. Fix well (at least 20% longer than equivalent sized colon blocks) 2 Process using usual times 3 After trimming blocks, soak in cold water prior to sectioning. This should dramatically improve your sections Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 20 December 2012 1:48 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] thyroid processing Does anyone have some good tips to processing thyroid tissue from thyroidectomy specimens? There seems to be a fine line between underprocessing and overprocessing where the tissue becomes brittle. We currently process them on an overnite run where the tissue is in all reagents for 45 minutes. We have also tried a shorter run on the Peloris that takes about 6 hours. Would like to know what others are doing for more optimal tissue processing!! Thanks so much everyone! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From akemiat3377 <@t> yahoo.com Wed Dec 19 17:22:02 2012 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Dec 19 17:22:08 2012 Subject: [Histonet] Histotechnician Salary 2 In-Reply-To: <761E2B5697F795489C8710BCC72141FF03033C@ex07.net.ucsf.edu> References: <50D21D18.2020503@mclean.harvard.edu> <761E2B5697F795489C8710BCC72141FF03033C@ex07.net.ucsf.edu> Message-ID: <1355959322.33690.YahooMailNeo@web113815.mail.gq1.yahoo.com> Tim: ? I have worked in California since 1998, both in the SF Bay area and LA.? The cost of living is extremely high in these two areas compared to the rest of California, and certainly higher?than most of the country.? ? Working as a manager in clinical and bio-tech settings, I found it difficult to find a histologist that was well rounded in all aspects of histology and IHC labs.? The histologists that were suitable to hit the ground running were compensated accordingly.? The average pay range paid for a registered HT/HTL was $25.00 to $40.00 per hour. ? There are a few bio-tech companies in the SF Bay area that hire unregistered histo techs and pay them $15.00 an hour.? They primarily cut control tissues and do the less complicated assignments,? ? I also know of several clinical histology laboratories in LA and SF that hire unregistered histo tech's and pay them $15.00 to $20.00 an hour.? You have to understand that we do not require registered histo techs to work in a lab as long as they have a registered supervisor.? The quality of work is compromised because these techs do not have the knowledge of theory and practice,? You pretty much get what you pay for. ? I was told a couple of years ago from a very good source that a recent graduate of a LA?histology program who passed their HT registry with a year of experience was paid $25.00 an hour to start. ? Hope this helps. ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: "Morken, Timothy" To: Tim Wheelock ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 19, 2012 12:14 PM Subject: RE: [Histonet] Histotechnician Salary 2 Tim, it depends on the going rate in your area. What does your hospital pay a general histotech? How about other hospitals in the area. I'll bet your personnel dept has that data. In California it can range from $15 to $35 per hour for histotechs with 5 years experience. The lower is in the rural areas and the higher in the San Francisco or LA areas. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, December 19, 2012 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnician Salary 2 Hi again all: I should have mentioned that the position calls for doing classical neuropathology stains, but not IHC. We have our immunostains done by another lab. Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gonavy2003 <@t> gmail.com Thu Dec 20 02:47:45 2012 From: gonavy2003 <@t> gmail.com (Rick Tiefenauer) Date: Thu Dec 20 02:47:53 2012 Subject: [Histonet] RE: Stark Law Message-ID: Jennifer, Did some reading up on Stark Law, couldn't find anything specific pertaining to CA other than Stark was a CA Congressman. The Stark Law is similar to the Federal Anti-Kickback Law. It is named after Pete Stark (congressman, Democrat, CA); he sponsored the original bill. One Reference I found is: "Stark Law Overview" by:GE Healthcare, 3000 N. Grandview Blvd., Waukesha, WI 53188 It can be viewed at: http://www.gehealthcare.com/usen/community/reimbursement/docs/FinalStarkLawTool_2011_doc0905301.pdf This Reference States: "In summary, the Stark Law prohibits a physician (or an immediate family member of such physician) who has a ?financial relationship? (including compensation and investment / ownership interests) with an entity from referring (broadly defined) patients to the entity for ?designated health services? covered by the Medicare program, unless an exception is available. In the event a proscribed referral is made and no exception is available, the entity performing the services is prohibited from submitting a claim for the services to the Medicare program or billing any individual, third-party payor or other entity for the services. In addition, certain aspects of the Stark Law apply to the state Medicaid programs". Another Reference, The AMA Website: http://www.ama-assn.org/ama/pub/physician-resources/legal-topics/regulatory-compliance-topics/stark-law-rules.page It is under the heading: "Resources ? Legal Issues ? Regulatory Compliance Topics ?Stark Law Rules" According to the AMA, a basic 6-step interactive guide to help physicians acquire an introductory knowledge of the Stark Law is available. More information from the AMA can be found at Stark Law "rules of the road" broadcast recording from July 13, 2012 webinar. Available at https://cc.readytalk.com/cc/playback/Playback.do?id=e0wier Hope this helps, Rick T. From TMcNemar <@t> lmhealth.org Thu Dec 20 04:47:35 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Dec 20 04:47:21 2012 Subject: [Histonet] 2 CAP questions In-Reply-To: <594004571.1636028.1355939134791.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <161372579.1635944.1355939041313.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> <594004571.1636028.1355939134791.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: All of our equipment is tied into the hospital emergency power system. Our tissue processor is also on a separate UPS backup just in case. Since we are a dayshift operation only and separated from the main lab, the processor is also monitored by the laboratory alarm system in the event of an overnight error condition. If there is a problem, I get a call. All laboratory thermometers (and timers) are calibrated yearly by an outside company that also covers pms and repair work. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Wednesday, December 19, 2012 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 2 CAP questions Fellow techs, 1) what type of a back up power source are you using for your tissue processors, refridgerators and freezors? 2) What do you do about calibration for NIST thermometers and non certified thermometers? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From vikk_0521 <@t> yahoo.com Thu Dec 20 11:22:04 2012 From: vikk_0521 <@t> yahoo.com (Vikrant Piprode) Date: Thu Dec 20 11:22:09 2012 Subject: [Histonet] (no subject) Message-ID: <1356024124.80894.YahooMailNeo@web162905.mail.bf1.yahoo.com> hi? i want to know the best method to decalcify calvarial bone of mice for subsequent osteoclast staining and goldner's trichrome staining. From TGoins <@t> mt.gov Thu Dec 20 11:45:15 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Dec 20 11:46:16 2012 Subject: [Histonet] (no subject) In-Reply-To: <1356024124.80894.YahooMailNeo@web162905.mail.bf1.yahoo.com> References: <1356024124.80894.YahooMailNeo@web162905.mail.bf1.yahoo.com> Message-ID: We recently switched to ion exchange resin with great results: American MasterTech I.E.D. Unit (Immuno) - Ion-Exchange Decalcification Unit Items: DCIEDIM60CS, DCIEDIM250CS, DCIEDIM250EA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vikrant Piprode Sent: Thursday, December 20, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) hi? i want to know the best method to decalcify calvarial bone of mice for subsequent osteoclast staining and goldner's trichrome staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwstarbu <@t> mdanderson.org Thu Dec 20 11:57:16 2012 From: mwstarbu <@t> mdanderson.org (Starbuck,Michael W) Date: Thu Dec 20 11:57:19 2012 Subject: [Histonet] (no subject) In-Reply-To: <1356024124.80894.YahooMailNeo@web162905.mail.bf1.yahoo.com> References: <1356024124.80894.YahooMailNeo@web162905.mail.bf1.yahoo.com> Message-ID: <12D0AD3D92A01A4783DB016AFC9A160C0124CE@DCPWPEXMBX02.mdanderson.edu> If you want to do TRAP staining for osteoclasts you will need to use 10 -15% EDTA at neutral pH to decalcify. It will take time but using agitation and changing the solution daily will facilitate the decalcification. Mike Michael Starbuck, M.S. Laboratory Coordinator Bone Disease Program of Texas Histomorphometry Core GU Medical Oncology The University of Texas M. D. Anderson Cancer Center T7.3966 ph: 713 563-1212 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vikrant Piprode Sent: Thursday, December 20, 2012 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) hi? i want to know the best method to decalcify calvarial bone of mice for subsequent osteoclast staining and goldner's trichrome staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Dec 20 12:40:33 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Dec 20 12:40:36 2012 Subject: [Histonet] PAI-1 In-Reply-To: <50D1F43F.7400.0077.1@harthosp.org> Message-ID: <14E2C6176416974295479C64A11CB9AE016411ABF680@SBS2K8.premierlab.local> Richard We have worked with two different antibodies in animals, rats and mice I think. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, December 19, 2012 3:07 PM To: Histonet Subject: [Histonet] PAI-1 Does anyone do IHC for PAI-1? Thank you. Happy Holidays to all and best wishes for 2013. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Dec 20 12:42:24 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Dec 20 12:42:30 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech Message-ID: From: Kim Donadio Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "O'Donnell, Bill" , Maria Mejia , "Morken, Timothy" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Kim Donadio (where?) comments: >>I'd like to add my two cents to the measuring "Quality" topic. I'll make it short. - You should have a "Quality Management" program. It's vital to track errors, types of errors, frequency and who etc. This is NOT a tool for blame as we are all adults or we should be. It is however a tool for tracking trends, making improvements and yes if you did see someone making a mistake often, you would have the data to educate particular personnel. - There are QM tools out there from various organizations. And yes, there are standards of deviations such as the TAT for frozens. There are standards for other things as well. Set Standards of excellence with your Pathologist. Make goals. Track them. Follow improvement.<< I'm going to add a sour and cynical rejoinder. As most of you know, I'm an elderly pathologist who's spent the last 30 years as a locum tenens, working maybe 60 pathology services in my "career". Sometimes in a pathology practice I'll be asked to fill out daily "quality whatever" (the patter changes with the years) reports about the slides. I always dread having to do this, because I know that the more of this paperwork I have to do, the worse the slides will be. The worst was one that required several square inches of scribbling a day. They couldn't mount a coverslip correctly. Any meaningful system would require daily feedback from pathologist (or other end user) to histotechnologist. I've never encountered a pathology service that accomplished this. Dang - now I'm remembering that this morning duodenal biopsy sections were the best I'd ever seen here, and I forgot to tell the histotech before she slipped out the door! Bob Richmond Samurai Pathologist Maryville TN From LPaveli1 <@t> hurleymc.com Thu Dec 20 13:05:58 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Dec 20 13:06:06 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: References: Message-ID: <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com> Dr. Richmond, It's always refreshing to hear what the "other shoe" has to say! I'm sure seeing those required quality control sheets coming in everyday is tiring, and then especially frustrating when small to none results are seen in a timely manner. I can empathize with you as change/improvement can sometimes take a long time!! I also realize.......after being in the field 40+ years (ouch!), that verbal communication seems to work faster than all those required sheets of paper you have to fill out. Like the pathologist who comes through the door saying; "HEY.......what happened with this slide??" (LOL) will get a much faster response/correction than those papers I receive back everyday to collate! It's just more personal, and shows the techs more of the pathologist's side of the hardships of diagnosing after receiving less than lovely slides. Equally refreshing, is a pathologist who remembers to thank the tech who does a great job! And I thank you for that! A genuine complement is really appreciated! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Thursday, December 20, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Basis for Quality Work in a Histotech From: Kim Donadio Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "O'Donnell, Bill" , Maria Mejia , "Morken, Timothy" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Kim Donadio (where?) comments: >>I'd like to add my two cents to the measuring "Quality" topic. I'll make it short. - You should have a "Quality Management" program. It's vital to track errors, types of errors, frequency and who etc. This is NOT a tool for blame as we are all adults or we should be. It is however a tool for tracking trends, making improvements and yes if you did see someone making a mistake often, you would have the data to educate particular personnel. - There are QM tools out there from various organizations. And yes, there are standards of deviations such as the TAT for frozens. There are standards for other things as well. Set Standards of excellence with your Pathologist. Make goals. Track them. Follow improvement.<< I'm going to add a sour and cynical rejoinder. As most of you know, I'm an elderly pathologist who's spent the last 30 years as a locum tenens, working maybe 60 pathology services in my "career". Sometimes in a pathology practice I'll be asked to fill out daily "quality whatever" (the patter changes with the years) reports about the slides. I always dread having to do this, because I know that the more of this paperwork I have to do, the worse the slides will be. The worst was one that required several square inches of scribbling a day. They couldn't mount a coverslip correctly. Any meaningful system would require daily feedback from pathologist (or other end user) to histotechnologist. I've never encountered a pathology service that accomplished this. Dang - now I'm remembering that this morning duodenal biopsy sections were the best I'd ever seen here, and I forgot to tell the histotech before she slipped out the door! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 20 13:20:31 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 20 13:21:52 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com> References: <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0304DC@ex07.net.ucsf.edu> We have a QC sheet but it is only used when the pathologist/resident sees something out of whack. We don't QC every single slide or even case. Remember also that someone has to review all those forms or it's a waste of time so the fewer the better! The result is we have about one QC sheet every other day (1500 hundred slides/day between H&E, IHC and SS). Fully half are submitted by one pathologist. That pathologist is also the ONLY one who submits them for good as well as poor slides (though in about a 10:1 ration bad:good). I keep track of them on a spreadsheet and do a review with the tech involved (f there is one). It is pretty random as to which techs have "errors," and all have at least one during a year's time, but we have picked up a few sectioning and stain problem trends over the years. It seems to work ok and it does not seem to be a burden. I also talk regularly with individual pathologists and just randomly ask them if they have seen any problems they want me to work on. I pick up a few issues that way. Of course if it is something they feel is really a problem I get the phone call... Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 20, 2012 11:06 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech Dr. Richmond, It's always refreshing to hear what the "other shoe" has to say! I'm sure seeing those required quality control sheets coming in everyday is tiring, and then especially frustrating when small to none results are seen in a timely manner. I can empathize with you as change/improvement can sometimes take a long time!! I also realize.......after being in the field 40+ years (ouch!), that verbal communication seems to work faster than all those required sheets of paper you have to fill out. Like the pathologist who comes through the door saying; "HEY.......what happened with this slide??" (LOL) will get a much faster response/correction than those papers I receive back everyday to collate! It's just more personal, and shows the techs more of the pathologist's side of the hardships of diagnosing after receiving less than lovely slides. Equally refreshing, is a pathologist who remembers to thank the tech who does a great job! And I thank you for that! A genuine complement is really appreciated! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Thursday, December 20, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Basis for Quality Work in a Histotech From: Kim Donadio Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "O'Donnell, Bill" , Maria Mejia , "Morken, Timothy" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Kim Donadio (where?) comments: >>I'd like to add my two cents to the measuring "Quality" topic. I'll >>make it short. - You should have a "Quality Management" program. It's >>vital to track errors, types of errors, frequency and who etc. This is >>NOT a tool for blame as we are all adults or we should be. It is >>however a tool for tracking trends, making improvements and yes if you >>did see someone making a mistake often, you would have the data to >>educate particular personnel. - There are QM tools out there from >>various organizations. And yes, there are standards of deviations such >>as the TAT for frozens. There are standards for other things as well. >>Set Standards of excellence with your Pathologist. Make goals. Track >>them. Follow improvement.<< I'm going to add a sour and cynical rejoinder. As most of you know, I'm an elderly pathologist who's spent the last 30 years as a locum tenens, working maybe 60 pathology services in my "career". Sometimes in a pathology practice I'll be asked to fill out daily "quality whatever" (the patter changes with the years) reports about the slides. I always dread having to do this, because I know that the more of this paperwork I have to do, the worse the slides will be. The worst was one that required several square inches of scribbling a day. They couldn't mount a coverslip correctly. Any meaningful system would require daily feedback from pathologist (or other end user) to histotechnologist. I've never encountered a pathology service that accomplished this. Dang - now I'm remembering that this morning duodenal biopsy sections were the best I'd ever seen here, and I forgot to tell the histotech before she slipped out the door! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Dec 20 14:08:37 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Dec 20 14:08:58 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: <761E2B5697F795489C8710BCC72141FF0304DC@ex07.net.ucsf.edu> References: <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com> <761E2B5697F795489C8710BCC72141FF0304DC@ex07.net.ucsf.edu> Message-ID: <053001cddeed$cd304300$6790c900$@pathview.com> I really appreciate this conversation because it show's an interaction between different members of the team. I sometimes think people forget that there are other team members besides just the histotechnologists or just the pathologists or just the pathology assistants, etc., etc. The only other point I'd like to add from an LIS vendor perspective is that the next time you purchase an LIS or a tracking system, or some other computerized solution, that's the time you want to use to ensure that it's easy to log quality or other 'actionable' issues and that those same indicators are easily available to ALL team members. As others have said, quality has to be part of the process. In order to increase the likelihood of a system's use, it needs to be easy to use and communicate. If it takes too many sheets of paper, too much time, or if it's just a painful process where no one sees any benefit, then it's doomed to a very uphill battle. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, December 20, 2012 11:21 AM To: Lynette Pavelich; Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech We have a QC sheet but it is only used when the pathologist/resident sees something out of whack. We don't QC every single slide or even case. Remember also that someone has to review all those forms or it's a waste of time so the fewer the better! The result is we have about one QC sheet every other day (1500 hundred slides/day between H&E, IHC and SS). Fully half are submitted by one pathologist. That pathologist is also the ONLY one who submits them for good as well as poor slides (though in about a 10:1 ration bad:good). I keep track of them on a spreadsheet and do a review with the tech involved (f there is one). It is pretty random as to which techs have "errors," and all have at least one during a year's time, but we have picked up a few sectioning and stain problem trends over the years. It seems to work ok and it does not seem to be a burden. I also talk regularly with individual pathologists and just randomly ask them if they have seen any problems they want me to work on. I pick up a few issues that way. Of course if it is something they feel is really a problem I get the phone call... Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 20, 2012 11:06 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech Dr. Richmond, It's always refreshing to hear what the "other shoe" has to say! I'm sure seeing those required quality control sheets coming in everyday is tiring, and then especially frustrating when small to none results are seen in a timely manner. I can empathize with you as change/improvement can sometimes take a long time!! I also realize.......after being in the field 40+ years (ouch!), that verbal communication seems to work faster than all those required sheets of paper you have to fill out. Like the pathologist who comes through the door saying; "HEY.......what happened with this slide??" (LOL) will get a much faster response/correction than those papers I receive back everyday to collate! It's just more personal, and shows the techs more of the pathologist's side of the hardships of diagnosing after receiving less than lovely slides. Equally refreshing, is a pathologist who remembers to thank the tech who does a great job! And I thank you for that! A genuine complement is really appreciated! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Thursday, December 20, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Basis for Quality Work in a Histotech From: Kim Donadio Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "O'Donnell, Bill" , Maria Mejia , "Morken, Timothy" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Kim Donadio (where?) comments: >>I'd like to add my two cents to the measuring "Quality" topic. I'll >>make it short. - You should have a "Quality Management" program. It's >>vital to track errors, types of errors, frequency and who etc. This is >>NOT a tool for blame as we are all adults or we should be. It is >>however a tool for tracking trends, making improvements and yes if you >>did see someone making a mistake often, you would have the data to >>educate particular personnel. - There are QM tools out there from >>various organizations. And yes, there are standards of deviations such >>as the TAT for frozens. There are standards for other things as well. >>Set Standards of excellence with your Pathologist. Make goals. Track >>them. Follow improvement.<< I'm going to add a sour and cynical rejoinder. As most of you know, I'm an elderly pathologist who's spent the last 30 years as a locum tenens, working maybe 60 pathology services in my "career". Sometimes in a pathology practice I'll be asked to fill out daily "quality whatever" (the patter changes with the years) reports about the slides. I always dread having to do this, because I know that the more of this paperwork I have to do, the worse the slides will be. The worst was one that required several square inches of scribbling a day. They couldn't mount a coverslip correctly. Any meaningful system would require daily feedback from pathologist (or other end user) to histotechnologist. I've never encountered a pathology service that accomplished this. Dang - now I'm remembering that this morning duodenal biopsy sections were the best I'd ever seen here, and I forgot to tell the histotech before she slipped out the door! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Thu Dec 20 14:12:12 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Dec 20 14:12:02 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com> References: <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939593FE73@chimsx08.CHI.catholichealth.net> I agree that the verbal approach is swiftest. I remember one lab that I worked in where we did daily QA sheets for overall quality. Specifics might be noted, but since I didn't always get the sheets back the same day - they weren't of much real help. Anyway, one day the pathologists tells me he thinks I need to look at the hematoxylin as it was staining "funny". Sure enough, it was. I inquired when he first noticed it and he said "oh, it's been several days". I looked at the sheets and everything was marked satisfactory. I got rid of the sheets, documented my own daily checks, and simply asked the paths how they thought things looked each day. They reviewed and signed off on the sheets at the end of the month, but I got first hand feedback which actually meant something and problems got solved in a more timely manner. I'm sure this experience is not unique, but since that event I have always insisted that the pathologist come directly to the lab when they have a problem with any aspect of the work. It's amazing what a little communication will do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 20, 2012 1:06 PM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech Dr. Richmond, It's always refreshing to hear what the "other shoe" has to say! I'm sure seeing those required quality control sheets coming in everyday is tiring, and then especially frustrating when small to none results are seen in a timely manner. I can empathize with you as change/improvement can sometimes take a long time!! I also realize.......after being in the field 40+ years (ouch!), that verbal communication seems to work faster than all those required sheets of paper you have to fill out. Like the pathologist who comes through the door saying; "HEY.......what happened with this slide??" (LOL) will get a much faster response/correction than those papers I receive back everyday to collate! It's just more personal, and shows the techs more of the pathologist's side of the hardships of diagnosing after receiving less than lovely slides. Equally refreshing, is a pathologist who remembers to thank the tech who does a great job! And I thank you for that! A genuine complement is really appreciated! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Thursday, December 20, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Basis for Quality Work in a Histotech From: Kim Donadio Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "O'Donnell, Bill" , Maria Mejia , "Morken, Timothy" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Kim Donadio (where?) comments: >>I'd like to add my two cents to the measuring "Quality" topic. I'll >>make it short. - You should have a "Quality Management" program. It's >>vital to track errors, types of errors, frequency and who etc. This is >>NOT a tool for blame as we are all adults or we should be. It is >>however a tool for tracking trends, making improvements and yes if you >>did see someone making a mistake often, you would have the data to >>educate particular personnel. - There are QM tools out there from >>various organizations. And yes, there are standards of deviations such >>as the TAT for frozens. There are standards for other things as well. >>Set Standards of excellence with your Pathologist. Make goals. Track >>them. Follow improvement.<< I'm going to add a sour and cynical rejoinder. As most of you know, I'm an elderly pathologist who's spent the last 30 years as a locum tenens, working maybe 60 pathology services in my "career". Sometimes in a pathology practice I'll be asked to fill out daily "quality whatever" (the patter changes with the years) reports about the slides. I always dread having to do this, because I know that the more of this paperwork I have to do, the worse the slides will be. The worst was one that required several square inches of scribbling a day. They couldn't mount a coverslip correctly. Any meaningful system would require daily feedback from pathologist (or other end user) to histotechnologist. I've never encountered a pathology service that accomplished this. Dang - now I'm remembering that this morning duodenal biopsy sections were the best I'd ever seen here, and I forgot to tell the histotech before she slipped out the door! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From joelleweaver <@t> hotmail.com Thu Dec 20 14:30:20 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Dec 20 14:30:28 2012 Subject: [Histonet] Re: Basis for Quality Work in a Histotech In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939593FE73@chimsx08.CHI.catholichealth.net> References: , <89F4666A496DC949A819ECC40E11C867BF56E6C7@EXCHANGEMB1.hmc.hurleymc.com>, <4940DF6D1C5FDF48931B6966AAEF939593FE73@chimsx08.CHI.catholichealth.net> Message-ID: I like that. Might be more difficult in a larger lab or facility however. I miss direct communication, and find it much more compelling than an email or reprimand after the fact. One of the things I used to like the most about working in histology was knowing the pathologists , having them at least know my name, and getting their direct feedback, (either good or bad), so I could adjust what I was doing appropriately. Even better if they showed me what they saw under the microscope. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 20 Dec 2012 13:12:12 -0700 > From: billodonnell@catholichealth.net > To: LPaveli1@hurleymc.com; rsrichmond@gmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech > CC: > > I agree that the verbal approach is swiftest. I remember one lab that I > worked in where we did daily QA sheets for overall quality. Specifics > might be noted, but since I didn't always get the sheets back the same > day - they weren't of much real help. Anyway, one day the pathologists > tells me he thinks I need to look at the hematoxylin as it was staining > "funny". Sure enough, it was. > > I inquired when he first noticed it and he said "oh, it's been several > days". I looked at the sheets and everything was marked satisfactory. I > got rid of the sheets, documented my own daily checks, and simply asked > the paths how they thought things looked each day. They reviewed and > signed off on the sheets at the end of the month, but I got first hand > feedback which actually meant something and problems got solved in a > more timely manner. I'm sure this experience is not unique, but since > that event I have always insisted that the pathologist come directly to > the lab when they have a problem with any aspect of the work. It's > amazing what a little communication will do. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette > Pavelich > Sent: Thursday, December 20, 2012 1:06 PM > To: Bob Richmond; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech > > Dr. Richmond, > It's always refreshing to hear what the "other shoe" has to say! I'm > sure seeing those required quality control sheets coming in everyday is > tiring, and then especially frustrating when small to none results are > seen in a timely manner. I can empathize with you as change/improvement > can sometimes take a long time!! > > I also realize.......after being in the field 40+ years (ouch!), that > verbal communication seems to work faster than all those required sheets > of paper you have to fill out. Like the pathologist who comes through > the door saying; "HEY.......what happened with this slide??" (LOL) will > get a much faster response/correction than those papers I receive back > everyday to collate! It's just more personal, and shows the techs more > of the pathologist's side of the hardships of diagnosing after receiving > less than lovely slides. > > Equally refreshing, is a pathologist who remembers to thank the tech who > does a great job! And I thank you for that! A genuine complement is > really appreciated! > > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > > ph: 810.262.9948 > mobile: 810.444.7966 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond > [rsrichmond@gmail.com] > Sent: Thursday, December 20, 2012 1:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Basis for Quality Work in a Histotech > > From: Kim Donadio > Subject: Re: [Histonet] Basis for Quality Work in a Histotech > To: "O'Donnell, Bill" , Maria > Mejia > , "Morken, Timothy" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > Kim Donadio (where?) comments: > >>I'd like to add my two cents to the measuring "Quality" topic. I'll > >>make it short. - You should have a "Quality Management" program. It's > >>vital to track errors, types of errors, frequency and who etc. This is > > >>NOT a tool for blame as we are all adults or we should be. It is > >>however a tool for tracking trends, making improvements and yes if you > > >>did see someone making a mistake often, you would have the data to > >>educate particular personnel. - There are QM tools out there from > >>various organizations. And yes, there are standards of deviations such > > >>as the TAT for frozens. There are standards for other things as well. > >>Set Standards of excellence with your Pathologist. Make goals. Track > >>them. Follow improvement.<< > > I'm going to add a sour and cynical rejoinder. As most of you know, I'm > an elderly pathologist who's spent the last 30 years as a locum tenens, > working maybe 60 pathology services in my "career". Sometimes in a > pathology practice I'll be asked to fill out daily "quality whatever" > (the patter changes with the years) reports about the slides. I always > dread having to do this, because I know that the more of this paperwork > I have to do, the worse the slides will be. The worst was one that > required several square inches of scribbling a day. > They couldn't mount a coverslip correctly. > > Any meaningful system would require daily feedback from pathologist (or > other end user) to histotechnologist. I've never encountered a pathology > service that accomplished this. > > Dang - now I'm remembering that this morning duodenal biopsy sections > were the best I'd ever seen here, and I forgot to tell the histotech > before she slipped out the door! > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dprieto <@t> fcien.edu.uy Thu Dec 20 14:45:13 2012 From: dprieto <@t> fcien.edu.uy (Daniel Prieto) Date: Thu Dec 20 14:45:20 2012 Subject: [Histonet] Connective tissue staining for araldite sections Message-ID: Dear all, I have been looking for a protocol for staining connective tissue on semithin (0.5 um) araldite-embedded sections. I found at least two protocols mentioning double staining (methylene blue/basic fuchsin) but turns out that only their abstracts are available. Any experience with this? I am trying to determine whether connective tissue has invaded muscle territory or muscle degeneration is taking place. I know about Heidenheins's azan trichrome, but it requires paraffin embedding (does it?) and it would be better if I could do it on the araldite sections. Thanks to all, Daniel From sccrshlly <@t> yahoo.com Thu Dec 20 15:29:15 2012 From: sccrshlly <@t> yahoo.com (Michelle Coker) Date: Thu Dec 20 15:29:26 2012 Subject: [Histonet] RE: Basis for Quality Work in a Histotech In-Reply-To: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> References: <4C076ACEE4E24575AC7BDF2CBE459FA6@Peterson.local> <1355589485.29574.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF02FDEC@ex07.net.ucsf.edu> <43A1727F-3B45-46B0-A5F8-4638A38440B9@gmail.com> <4940DF6D1C5FDF48931B6966AAEF93958EFCD6@chimsx08.CHI.catholichealth.net> <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Message-ID: <6F614D07-D5EF-4E88-9290-29784FA4699C@yahoo.com> One thing to remember when addressing quality issues in the lab is to find a root cause. If the same mistakes are being made over and over by both techs, then that isn't a personnel problem that can be fixed with rewards/punishment. That is a process problem. You need to review you processes and will likely find the root cause for your errors there. Regards, Sent from my iPad On Dec 19, 2012, at 2:17 PM, Kim Donadio wrote: > Id like to add my 2cents to the measuring "Quality" topic. I'll make it short. > > You should have a "Quality Managment" program. It's vital to track errors, trypes of errors, frequency and who etc. This is NOT a tool for blame as we are all adults or we should be. It is however a tool for tracking trends, making improvments and yes if you did see someone making a mistake often, you would have the data to educate particular personel. > > There are QM tools out there from various organizations. And yes, there are standards of deviations such as the TAT for frozens. There are standards for other thangs as well. Set Standards of excellence with your Pathologist. Make goals. Track them. Follow improvment. > > So try a google on QM and see if that helps answer any of the original questions. > > BTW... Merry Christmas Histonettters. :) > > Kim :D > > ________________________________ > From: "O'Donnell, Bill" > To: Maria Mejia ; "Morken, Timothy" > Cc: histonet@lists.utsouthwestern.edu > Sent: Tuesday, December 18, 2012 2:37 PM > Subject: RE: [Histonet] Basis for Quality Work in a Histotech > > Maria - Well said. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Mejia > Sent: Tuesday, December 18, 2012 11:36 AM > To: Morken, Timothy > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Basis for Quality Work in a Histotech > > Travis, > > I agree with everyone's valuable thoughts regarding your question. As a supervisor, it's imperative to communicate with people in such a way that they change themselves. > With some people the act of "getting the task done," has such an urgent need that can lead them to become careless & aggressive, leaping before looking & even speaking without thinking first. In histology, we know it's more important to avoid making mistakes - to be certain every detail is accurate & in place. And, it's important to find the balance between the 2 intentions of "getting the task done" & "getting it done right!" > > It's also important to create & develop relationships with those you work with. The desire to contribute to others & be appreciated for what you do, is one of the most powerful > motivational forces known. And yes, sometimes you get what you give! Giving appreciation & getting along with others go hand-in-hand - & as a supervisor it's another balancing > act. Also when people have the same priorities, a misunderstanding or conflict is highly UNLIKELY. > > All the best > Maria Mejia > San Francisco, CA > > > On Dec 17, 2012, at 8:30 AM, Morken, Timothy wrote: > >> Travis, >> >> Histology has a very complex workflow AND requires "artisan" level workmanship to deliver a product. Those two together nearly guarantee mistakes, mostly minor, but sometimes literally life-threatening to patients. The goal is to instill a sense of Best Quality in the techs. A large part of achieving that attitude is to ensure the pathologists and administrators are behind the techs 100% and ALLOW the techs to do Best Quality - ie, accept that Best Quality will sometimes mean slower turnaround time. Does that aspect mean more people are needed? That's your call, but can be determined by workload accounting. >> >> The attitude should be that the SYSTEM makes the mistake, not the individual. It is not likely a person makes a mistake on purpose, but instead is it some aspect of the system that allows them to make a mistake (though "shortcuts" can be thought of as intentionally risking making mistakes "on purpose," the "purpose" being to save time or effort). >> >> Workflows can be "engineered" to ensure some mistakes don't happen. Protocols must be followed to the letter by EVERYONE. No workarounds allowed (a workaround is an indication that there is something wrong in the system - the employee feels the need to take shortcuts. Why? BTW, Bill Gates said the most important word in his vocabulary is "why." Why is something done the way it is? Why does a mistake happen at a certain point? ). In failure analysis a problem is approached by asking 5 levels of WHY? After asking WHY 5 times back down the workflow chain you usually find the root cause of a problem. If not, you keep asking why until the root cause is found. >> >> For instance, we worked out a slide labeling protocol at the microtome that, if followed, will ensure the tech does not make labeling errors. All participated in working this out and so have bought into the system. All new employees are trained in that system. That will eventually be followed by barcoding, but that is a year away at least. But our protocol has nearly eliminated labeling errors (we still get a few sneaking in here and there but as we catch them we try to figure out how to engineer them away). >> >> We also finally instituted the printing of cassettes directly from our LIS rather than using a stand-alone printer or hand-writing. That has almost totally eliminated cassette labeling errors - we used to have hundreds per month, mainly by residents putting in cassettes that they did not enter in our LIS, or making simple typo errors on a stand-alone cassette labeler, or hand-written cassettes. >> >> All these methods need to be investigated. >> >> Rewards are also very helpful. We give out "Bear hugs" that are $5 gift certificates to the campus store, cafeteria, various food vendors in the institution, etc. it's a small reward, but people actually appreciate it. We also have "Star Awards" of $50 gift cards for those times when someone does something more beyond the usual. The receiver chooses the card they want from about 2 dozen available (coffee shops, VISA, various stores, etc). >> >> >> Good luck with it! >> >> >> Tim Morken >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology UC San Francisco Medical Center >> >> >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J >> Buesa >> Sent: Saturday, December 15, 2012 8:38 AM >> To: Travis Troyer; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Basis for Quality Work in a Histotech >> >> First I want you to excuse me, but I do not think that you are really qualified to supervise 2 histotechs if you need to ask for such quality guidance. >> You end by bemoaning about "budget crunch" and because of that it seems to me that your 2 histotechs are not receiving a "decent" salary and, as everybody knows, you are getting what you are paying for. >> With 10 years of experience you should know that the first step for quality of sections is quality of fixation and quality of processing. You have first to manage that aspect. >> Quality of sections comes after wards and there is no "standard" for mistakes and for what you are describing it seems that mistakes are frequent. By the way, if the pathologists are not pleased, they will not it take on the histotechs, but on you as a supervisor unable to provide them the quality they require. >> There is no such thing as "instant reward" for a good quality job; the histotech should not be treated as "dogs receiving a cookie after a trick performed" but there are 2 tools: you need to keep track of the mistakes ? counsel the HT after a mistake ? retrain them ? keep a track of mistakes and there are verbal and written counselings and an annual evaluation, I am sure you know that. >> The ideal limit of mistakes is "0" but there is some acceptable mistakes limits, as long as they are few and far between. The pathologists are the ones who can tell you what they are willing to accept as mistakes limits. Ask them. >> It seems that if your 2 HTs do not improve, you should start looking for replacements, but they should be better paid, and if the mistakes continue at a high rate, you should put your 10 years experience to work and start doing some bench work Ren? J. >> >> >> From: Travis Troyer >> To: histonet@lists.utsouthwestern.edu >> Sent: Friday, December 14, 2012 5:34 PM >> Subject: [Histonet] Basis for Quality Work in a Histotech >> >> This is a question for all of the lab supervisors. I am the supervisor of two histotechs. I am not doing techwork now, but have 10 years of experience. The pathologists are getting more and more upset at the lack of quality in the work and the mistakes that are happening. I was wondering if anyone had some ideas on what sort of a goal to set up and how to reward/punish for variations from that goal. For example, if the goal is three mistakes for the month, what is the best way to reward them for making that goal and what would be best if they had more mistakes in a given time frame. We are all feeling the budget crunch and the pathologists are trying to figure out a good solution. >> >> Thanks, >> Travis Troyer >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Dec 21 02:50:13 2012 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Fri Dec 21 02:50:25 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUmU6IEJhc2lzIGZvciBRdWFsaXR5IFdvcmsgaW4gYSBI?= =?utf-8?B?aXN0b3RlY2g=?= Message-ID: Good points Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Michael Mihalik" To: "'Morken, Timothy'" , "'Lynette Pavelich'" , "'Bob Richmond'" , Subject: [Histonet] Re: Basis for Quality Work in a Histotech Date: Thu, Dec 20, 2012 2:08 pm I really appreciate this conversation because it show's an interaction between different members of the team. I sometimes think people forget that there are other team members besides just the histotechnologists or just the pathologists or just the pathology assistants, etc., etc. The only other point I'd like to add from an LIS vendor perspective is that the next time you purchase an LIS or a tracking system, or some other computerized solution, that's the time you want to use to ensure that it's easy to log quality or other 'actionable' issues and that those same indicators are easily available to ALL team members. As others have said, quality has to be part of the process. In order to increase the likelihood of a system's use, it needs to be easy to use and communicate. If it takes too many sheets of paper, too much time, or if it's just a painful process where no one sees any benefit, then it's doomed to a very uphill battle. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, December 20, 2012 11:21 AM To: Lynette Pavelich; Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech We have a QC sheet but it is only used when the pathologist/resident sees something out of whack. We don't QC every single slide or even case. Remember also that someone has to review all those forms or it's a waste of time so the fewer the better! The result is we have about one QC sheet every other day (1500 hundred slides/day between H&E, IHC and SS). Fully half are submitted by one pathologist. That pathologist is also the ONLY one who submits them for good as well as poor slides (though in about a 10:1 ration bad:good). I keep track of them on a spreadsheet and do a review with the tech involved (f there is one). It is pretty random as to which techs have "errors," and all have at least one during a year's time, but we have picked up a few sectioning and stain problem trends over the years. It seems to work ok and it does not seem to be a burden. I also talk regularly with individual pathologists and just randomly ask them if they have seen any problems they want me to work on. I pick up a few issues that way. Of course if it is something they feel is really a problem I get the phone call... Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 20, 2012 11:06 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Basis for Quality Work in a Histotech Dr. Richmond, It's always refreshing to hear what the "other shoe" has to say! I'm sure seeing those required quality control sheets coming in everyday is tiring, and then especially frustrating when small to none results are seen in a timely manner. I can empathize with you as change/improvement can sometimes take a long time!! I also realize.......after being in the field 40+ years (ouch!), that verbal communication seems to work faster than all those required sheets of paper you have to fill out. Like the pathologist who comes through the door saying; "HEY.......what happened with this slide??" (LOL) will get a much faster response/correction than those papers I receive back everyday to collate! It's just more personal, and shows the techs more of the pathologist's side of the hardships of diagnosing after receiving less than lovely slides. Equally refreshing, is a pathologist who remembers to thank the tech who does a great job! And I thank you for that! A genuine complement is really appreciated! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Thursday, December 20, 2012 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Basis for Quality Work in a Histotech From: Kim Donadio Subject: Re: [Histonet] Basis for Quality Work in a Histotech To: "O'Donnell, Bill" , Maria Mejia , "Morken, Timothy" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1355948250.57406.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Kim Donadio (where?) comments: >>I'd like to add my two cents to the measuring "Quality" topic. I'll >>make it short. - You should have a "Quality Management" program. It's >>vital to track errors, types of errors, frequency and who etc. This is >>NOT a tool for blame as we are all adults or we should be. It is >>however a tool for tracking trends, making improvements and yes if you >>did see someone making a mistake often, you would have the data to >>educate particular personnel. - There are QM tools out there from >>various organizations. And yes, there are standards of deviations such >>as the TAT for frozens. There are standards for other things as well. >>Set Standards of excellence with your Pathologist. Make goals. Track >>them. Follow improvement.<< I'm going to add a sour and cynical rejoinder. As most of you know, I'm an elderly pathologist who's spent the last 30 years as a locum tenens, working maybe 60 pathology services in my "career". Sometimes in a pathology practice I'll be asked to fill out daily "quality whatever" (the patter changes with the years) reports about the slides. I always dread having to do this, because I know that the more of this paperwork I have to do, the worse the slides will be. The worst was one that required several square inches of scribbling a day. They couldn't mount a coverslip correctly. Any meaningful system would require daily feedback from pathologist (or other end user) to histotechnologist. I've never encountered a pathology service that accomplished this. Dang - now I'm remembering that this morning duodenal biopsy sections were the best I'd ever seen here, and I forgot to tell the histotech before she slipped out the door! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Dec 21 13:29:59 2012 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Dec 21 13:30:03 2012 Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! Message-ID: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> Merry Christmas to everyone from the Heart of Dixie! And thank you Dr Margraf and Marvin Hanna for hosting the "Histonet". It is a real asset to histotechnologists. Michael Titford Pathology USA Mobile AL From tony.henwood <@t> health.nsw.gov.au Sun Dec 23 16:07:32 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Dec 23 16:07:57 2012 Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! In-Reply-To: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> References: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D20ED0E@xmdb04.nch.kids> I also strongly agree. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Saturday, 22 December 2012 6:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! Merry Christmas to everyone from the Heart of Dixie! And thank you Dr Margraf and Marvin Hanna for hosting the "Histonet". It is a real asset to histotechnologists. Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From joelleweaver <@t> hotmail.com Sun Dec 23 16:46:00 2012 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Sun Dec 23 16:46:07 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTWVycnkgQ2hyaXN0bWFzLCBhbmQgdGhhbmtzIERyIE1h?= =?utf-8?B?cmdyYWYh?= Message-ID: Yes we are all fortunate to have this forum. Merry Christmas to all histonetters! Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Tony Henwood (SCHN)" To: "'mtitford@aol.com'" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! Date: Sun, Dec 23, 2012 4:07 pm I also strongly agree. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Saturday, 22 December 2012 6:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! Merry Christmas to everyone from the Heart of Dixie! And thank you Dr Margraf and Marvin Hanna for hosting the "Histonet". It is a real asset to histotechnologists. Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon Dec 24 07:22:08 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Dec 24 07:22:16 2012 Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! In-Reply-To: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> References: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> Message-ID: I'd like to add my thanks as well - I am so grateful for this connection. Merry Christmas/Happy Holidays to all! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Friday, December 21, 2012 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! Merry Christmas to everyone from the Heart of Dixie! And thank you Dr Margraf and Marvin Hanna for hosting the "Histonet". It is a real asset to histotechnologists. Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rjbuesa <@t> yahoo.com Mon Dec 24 09:07:24 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 24 09:07:28 2012 Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D20ED0E@xmdb04.nch.kids> References: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> <6D6BD1DE8A5571489398B392A38A71579D20ED0E@xmdb04.nch.kids> Message-ID: <1356361644.31307.YahooMailNeo@web163103.mail.bf1.yahoo.com> Me too!!!!!!!!!!!!! Ren? J. From: Tony Henwood (SCHN) To: "'mtitford@aol.com'" ; "histonet@lists.utsouthwestern.edu" Sent: Sunday, December 23, 2012 5:07 PM Subject: RE: [Histonet] Merry Christmas, and thanks Dr Margraf! I also strongly agree. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Saturday, 22 December 2012 6:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! Merry Christmas to everyone from the Heart of Dixie! And thank you Dr Margraf and Marvin Hanna for hosting? the "Histonet". It is a real asset to histotechnologists. Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Margraf <@t> cookchildrens.org Mon Dec 24 09:15:33 2012 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Mon Dec 24 09:15:40 2012 Subject: [Histonet] RE: Histonet Digest, Vol 109, Issue 25 In-Reply-To: <0dcf4417-5c6f-434b-bc13-28f1e8d65462@CCHCSCAHT03.CCHCS.LDAP> References: <0dcf4417-5c6f-434b-bc13-28f1e8d65462@CCHCSCAHT03.CCHCS.LDAP> Message-ID: <928719B9EBFA1C4686918B975FF8452882D5BB53@CCHCSMBX04.CCHCS.LDAP> Hey Merry Christmas and Happy holidays to you all (ya'll) too. Also, thanks to Dr. Sandy Cope, the other Histonet administrator. Best wishes for a GREAT 2013, Linda M Histonet administrator Date: Fri, 21 Dec 2012 14:29:59 -0500 (EST) From: mtitford@aol.com Subject: [Histonet] Merry Christmas, and thanks Dr Margraf! To: histonet@lists.utsouthwestern.edu Message-ID: <8CFADC8B29D6121-8A0-3E241@webmail-d162.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Merry Christmas to everyone from the Heart of Dixie! And thank you Dr Margraf and Marvin Hanna for hosting the "Histonet". It is a real asset to histotechnologists. Michael Titford Pathology USA Mobile AL From Lisa.Chapman <@t> aurora.org Mon Dec 24 17:07:38 2012 From: Lisa.Chapman <@t> aurora.org (Chapman, Lisa) Date: Mon Dec 24 17:07:43 2012 Subject: [Histonet] Recommendations for H&E stainer? Message-ID: <8846E48BB7D51F439658082F0BC747D5A32F67@HEIMSEAT024.ahc.root.loc> Our lab is researching H&E stainers. Our main H&E stainer (Leica ST 4040) is not being produced by Leica anymore and is a workhorse. Our lab produces about 2500-3000 H&E slides a day. One item we have looked at in the past is the Prisma stainer by Sakura which you can hook up a coverslipper to (we already have the appropriate film coverslipper). One stainer would meet our current demands as far as turnaround time goes. It was suggested (and we agreed) that we would need three Prisma stainers linked together to meet our demands - however, we do not have the space for three stainers. All suggestions are welcome! Lisa Chapman, HT (ASCP) Second/Third Shift Histology Supervisor | ACL Laboratories 8901 West Lincoln Avenue West Allis, WI 53227 *414.939.1723 |7414.328.8505 *414.222.3929 |* lisa.chapman@aurora.org From annigyg <@t> gmail.com Mon Dec 24 23:53:37 2012 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Mon Dec 24 23:53:41 2012 Subject: [Histonet] Merry Christmas Message-ID: Annie in Africa aka ArabianAnnie is back and wishes you all a very Merry Christmas and festive New Year - Happy Holidays -- Anne van Binsbergen Abu Dhabi UAE From panduranglambade <@t> gmail.com Wed Dec 26 03:00:29 2012 From: panduranglambade <@t> gmail.com (Dr. pandurang Lambade) Date: Wed Dec 26 03:00:34 2012 Subject: [Histonet] Merry Christmas In-Reply-To: References: Message-ID: Merry Christmas to all Histonetres. i am new to the field can any one tell me PAS staining procedure for the staging of testis in a reptox study and how to take serial section of the ovaries. On Tue, Dec 25, 2012 at 11:23 AM, Anne van Binsbergen wrote: > Annie in Africa aka ArabianAnnie is back and wishes you all a very Merry > Christmas and festive New Year - Happy Holidays > -- > Anne van Binsbergen > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- With Regards *Dr. Pandurang Lambade* Junior Pathologist, SA-FORD (A Division of Sharon Bio-Medicine Ltd.) Plot V-10, Taloja -M.I.D.C. Dist-Raigad, Maharashtra- 402 208 Phone No.: 022 645644000/12/13 website: www.sa-ford.com P* **Go Green. Use less papers and maintain environment to live. This could help the world to reduce global warming.** Please Do not take a print unless you need it.* From victoria.spoon <@t> bassett.org Wed Dec 26 07:16:43 2012 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Wed Dec 26 07:16:48 2012 Subject: [Histonet] Morgue access Message-ID: <3D002D8531A0C743A35B817037E5AECA0C2936@EX08.bassett.org> What are other facilities policy on morgue access and patient release? Do you require photo identification every time a funeral home arrives to pick up a patient? Thank you From GauchV <@t> mail.amc.edu Wed Dec 26 08:05:43 2012 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Wed Dec 26 08:05:55 2012 Subject: [Histonet] MDM2 Probe/Ventana Message-ID: Hi, Does anyone have a protocol for the MDM2 Probe from Ventana that they could share ? We have been unable to locate one thus far and our IHC Technical Specialist needs one. Thank you in advance for your assistance, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From marktarango <@t> gmail.com Wed Dec 26 10:37:49 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Dec 26 10:37:53 2012 Subject: [Histonet] MDM2 Probe/Ventana In-Reply-To: References: Message-ID: Hi Vicki, I don't have a protocol but it should be very similar to the HER2 dual ISH. I'm assuming that you'll be using a probe targeted to chromosome 12 too and not just doing the MDM2 (but maybe I'm wrong). I would start there. I'm working on validating MDM2 FISH and it's nearly identical to our HER2 FISH protocol, right down to denaturation and hybridization times. Mark On Wed, Dec 26, 2012 at 6:05 AM, Gauch, Vicki wrote: > Hi, > Does anyone have a protocol for the MDM2 Probe from Ventana that they > could share ? We have been unable to locate one thus far and our IHC > Technical Specialist needs one. > Thank you in advance for your assistance, > > Vicki Gauch > AMCH > Albany, NY > > > > > ----------------------------------------- > CONFIDENTIALITY NOTICE: This email and any attachments may contain > confidential information that is protected by law and is for the > sole use of the individuals or entities to which it is addressed. > If you are not the intended recipient, please notify the sender by > replying to this email and destroying all copies of the > communication and attachments. Further use, disclosure, copying, > distribution of, or reliance upon the contents of this email and > attachments is strictly prohibited. To contact Albany Medical > Center, or for a copy of our privacy practices, please visit us on > the Internet at www.amc.edu. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWellborn <@t> bjc.org Wed Dec 26 15:39:40 2012 From: JWellborn <@t> bjc.org (Janci Wellborn) Date: Wed Dec 26 15:42:10 2012 Subject: [Histonet] Melanin Bleaching Message-ID: <50DB1A3C020000B2000E79E3@upbcgwg06> I am looking for alternatives to the potassium permanganate melanin bleaching procedure when doing IHC. I have seen literature references to doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa counterstain in the past. Does any one have either procedure that they would be willing to share? We are a small volume lab and the costs associated with bringing in a different color IHC kit is not justified. Any help would be greatly appreciated. Janci Janci R Wellborn, HTL (ASCP) Laboratory Technical Coordinator Histology Alton Memorial Hospital 1 Memorial Drive Alton, Ill 62002 618-463-7082 From rjbuesa <@t> yahoo.com Wed Dec 26 15:47:23 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 26 15:47:26 2012 Subject: [Histonet] Melanin Bleaching In-Reply-To: <50DB1A3C020000B2000E79E3@upbcgwg06> References: <50DB1A3C020000B2000E79E3@upbcgwg06> Message-ID: <1356558443.97578.YahooMailNeo@web163106.mail.bf1.yahoo.com> Melanin bleaching has to be done prior to the IHC procedure and the most reliable?way is using potassium permanganate. Any other option will produce less than optimal results. Ren? J. From: Janci Wellborn To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 26, 2012 4:39 PM Subject: [Histonet] Melanin Bleaching I am looking for alternatives to the potassium permanganate melanin bleaching procedure when doing IHC. I have seen literature references to doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa counterstain in the past. Does any one have either procedure that they would be willing to share? We are a small volume lab and the costs associated with bringing in a different color IHC kit is not justified. Any help would be greatly appreciated. Janci Janci R Wellborn, HTL (ASCP) Laboratory Technical Coordinator Histology Alton Memorial Hospital 1 Memorial Drive Alton, Ill 62002 618-463-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allen.Keeping <@t> albertahealthservices.ca Thu Dec 27 12:40:23 2012 From: Allen.Keeping <@t> albertahealthservices.ca (Allen Keeping D.) Date: Thu Dec 27 12:40:28 2012 Subject: [Histonet] Microtome maintenance frequency Message-ID: <1DC4211314F7D9458B66A28F947F60B901236E95DE29@EXMBX4C.crha.bewell.ca> I have a question regarding scheduled maintenance of rotary microtomes. I run a simulation lab for students of lab technology. Part of their histology rotation involves learning how to produce acceptable sections on the microtome. I currently have 3 Leica rotary (manual) microtomes, which are used by students a total of 18-20 days per year (for educational purposes, not to produce diagnostic materials). In addition, I may use a microtome for a couple weeks total each year to produce control materials and to troubleshoot staining issues. Currently, the microtomes are serviced by a Leica-educated professional annually for preventative maintenance. As these microtomes are only used infrequently, is this level of maintenance necessary (or recommended)? I am contemplating switching to a bi-annual schedule as a cost-saving measure. Does anyone have any advice or experience regarding maintaining microtomes in a low-volume setting? Cheers, Allen ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From melissa.koehnlein <@t> pds.usask.ca Thu Dec 27 13:04:29 2012 From: melissa.koehnlein <@t> pds.usask.ca (Koehnlein, Melissa) Date: Thu Dec 27 13:04:37 2012 Subject: [Histonet] Melanin Bleaching Message-ID: <87BF46BACB9B124EAD75F536E82A9BEDE0B4D2@CAMPUSMB5.usask.ca> Melanin can be distinguished from DAB by counterstaining with Azure B, which gives a dark green color to the melanin. We've used the procedure below with success: Immunoperoxidase technique modified by counterstain with azure B as a diagnostic aid in evaluating heavily pigmented melanocytic neoplasms: http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0560.1991.tb01381.x/abstract Melissa Koehnlein Lab Technician Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7223 Fax (306) 966-2488 Email mailto:melissa.koehnlein@pds.usask.ca ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, December 27, 2012 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Melanin Bleaching (Janci Wellborn) 2. Re: Melanin Bleaching (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 26 Dec 2012 15:39:40 -0600 From: "Janci Wellborn" Subject: [Histonet] Melanin Bleaching To: Message-ID: <50DB1A3C020000B2000E79E3@upbcgwg06> Content-Type: text/plain; charset="us-ascii" I am looking for alternatives to the potassium permanganate melanin bleaching procedure when doing IHC. I have seen literature references to doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa counterstain in the past. Does any one have either procedure that they would be willing to share? We are a small volume lab and the costs associated with bringing in a different color IHC kit is not justified. Any help would be greatly appreciated. Janci Janci R Wellborn, HTL (ASCP) Laboratory Technical Coordinator Histology Alton Memorial Hospital 1 Memorial Drive Alton, Ill 62002 618-463-7082 ------------------------------ Message: 2 Date: Wed, 26 Dec 2012 13:47:23 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Melanin Bleaching To: Janci Wellborn , "histonet@lists.utsouthwestern.edu" Message-ID: <1356558443.97578.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Melanin bleaching has to be done prior to the IHC procedure and the most reliable?way is using potassium permanganate. Any other option will produce less than optimal results. Ren? J. From: Janci Wellborn To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 26, 2012 4:39 PM Subject: [Histonet] Melanin Bleaching I am looking for alternatives to the potassium permanganate melanin bleaching procedure when doing IHC. I have seen literature references to doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa counterstain in the past. Does any one have either procedure that they would be willing to share? We are a small volume lab and the costs associated with bringing in a different color IHC kit is not justified. Any help would be greatly appreciated. Janci Janci R Wellborn, HTL (ASCP) Laboratory Technical Coordinator Histology Alton Memorial Hospital 1 Memorial Drive Alton, Ill 62002 618-463-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 109, Issue 29 ***************************************** From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 27 13:35:33 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 27 13:35:48 2012 Subject: [Histonet] RE: Microtome maintenance frequency In-Reply-To: <1DC4211314F7D9458B66A28F947F60B901236E95DE29@EXMBX4C.crha.bewell.ca> References: <1DC4211314F7D9458B66A28F947F60B901236E95DE29@EXMBX4C.crha.bewell.ca> Message-ID: <761E2B5697F795489C8710BCC72141FF0375BC@ex07.net.ucsf.edu> Allen, Yes the service contract is very expensive. In fact, at our hospital we decided to forgo the service contract on microtomes and only call for service when a microtome has a problem. That has saved us tens of thousands of dollars over the years. In the four years since we have done that we have only serviced 3 microtomes for a cost of about $4,000. The annual contract would have been $64,000 during that period! And in fact, the only problems we have had were related to electronic ribbon cables breaking from repeated bending - poor design by the manufacturer. We also have a 7 year replacement policy so they aren't going to get badly worn like some places that never replace them. The minimal use of your microtomes means they aren't going to have the wear that we see in the hospital. Your problem will be from oil or grease drying out and making the operation sticky or slow. Be sure you do any suggested daily, weekly or monthly maintenance outlined in the microtome manual. Also be sure to learn what signs indicate a problem with the microtome. It would be good to have someone use the microtome on a regular basis just to keep the mechanics smoothly operating - maybe once a month in your case. An accredited lab simply needs to document the regular maintenance and any repairs done to satisfy inspectors. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allen Keeping D. Sent: Thursday, December 27, 2012 10:40 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microtome maintenance frequency I have a question regarding scheduled maintenance of rotary microtomes. I run a simulation lab for students of lab technology. Part of their histology rotation involves learning how to produce acceptable sections on the microtome. I currently have 3 Leica rotary (manual) microtomes, which are used by students a total of 18-20 days per year (for educational purposes, not to produce diagnostic materials). In addition, I may use a microtome for a couple weeks total each year to produce control materials and to troubleshoot staining issues. Currently, the microtomes are serviced by a Leica-educated professional annually for preventative maintenance. As these microtomes are only used infrequently, is this level of maintenance necessary (or recommended)? I am contemplating switching to a bi-annual schedule as a cost-saving measure. Does anyone have any advice or experience regarding maintaining microtomes in a low-volume setting? Cheers, Allen ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 27 15:15:55 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 27 15:16:04 2012 Subject: [Histonet] Microtome maintenance frequency In-Reply-To: <1DC4211314F7D9458B66A28F947F60B901236E95DE29@EXMBX4C.crha.bewell.ca> References: <1DC4211314F7D9458B66A28F947F60B901236E95DE29@EXMBX4C.crha.bewell.ca> Message-ID: <1356642955.99063.YahooMailNeo@web163102.mail.bf1.yahoo.com> For your usage level? it is unnecessary an annual maintenance. As a matter of fact I am sure you will be able to use those Leica microtomes for many years ar the same usage level without needing any "capital"?maintenance. Ren? J. From: Allen Keeping D. To: "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, December 27, 2012 1:40 PM Subject: [Histonet] Microtome maintenance frequency I have a question regarding scheduled maintenance of rotary microtomes. I run a simulation lab for students of lab technology. Part of their histology rotation involves learning how to produce acceptable sections on the microtome. I currently have 3 Leica rotary (manual) microtomes, which are used by students a total of 18-20 days per year (for educational purposes, not to produce diagnostic materials). In addition, I may use a microtome for a couple weeks total each year to produce control materials and to troubleshoot staining issues. Currently, the microtomes are serviced by a Leica-educated professional annually for preventative maintenance. As these microtomes are only used infrequently, is this level of maintenance necessary (or recommended)? I am contemplating switching to a bi-annual schedule as a cost-saving measure. Does anyone have any advice or experience regarding maintaining microtomes in a low-volume setting? Cheers, Allen ? ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jim000001 <@t> hotmail.com Thu Dec 27 17:13:45 2012 From: jim000001 <@t> hotmail.com (Jim Jones) Date: Thu Dec 27 17:13:49 2012 Subject: [Histonet] Tissue Microarray Question Message-ID: Hello,Our lab is getting ready to construct TMAs to optimize new Immuno stainers. We purchased the Arraymold TMA instrument and was wondering if anyone has used it for this purpose? It looks fairly easy to use. ThanksJimmy From kimulah <@t> gmail.com Thu Dec 27 22:22:59 2012 From: kimulah <@t> gmail.com (=?ISO-2022-JP?B?GyRCTFpCPE01PHkbKEI=?=) Date: Thu Dec 27 22:23:04 2012 Subject: [Histonet] In permeabilization, can we use Tween-20 instead of Triton-100? Message-ID: Dear Histonetters Hi. Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL). The primary antibody is for detecting some intracellular antigen(antigen in cytoplasm). So I think permeabilization process may be necessary after fixation by 4% PFA. Does anyone know whether we can use Tween-20 in place of Triton-100 for permeabilization? And can we use same protocol in immunostaining of BAL cells as in immunostaining for cell line? Thanks in advance. H Kimura From ln0517 <@t> gmail.com Fri Dec 28 13:04:45 2012 From: ln0517 <@t> gmail.com (Ly Nguyen) Date: Fri Dec 28 13:04:52 2012 Subject: [Histonet] Help Message-ID: I want to be remove from the list. I've tried the unsubscribe link be low several time but it seem like it doesn't work because I'm still receiving daily email On Friday, December 28, 2012, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Microtome maintenance frequency (Allen Keeping D.) > 2. Melanin Bleaching (Koehnlein, Melissa) > 3. RE: Microtome maintenance frequency (Morken, Timothy) > 4. Re: Microtome maintenance frequency (Rene J Buesa) > 5. Tissue Microarray Question (Jim Jones) > 6. In permeabilization, can we use Tween-20 instead of > Triton-100? (????) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 27 Dec 2012 11:40:23 -0700 > From: Allen Keeping D. > Subject: [Histonet] Microtome maintenance frequency > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > < > 1DC4211314F7D9458B66A28F947F60B901236E95DE29@EXMBX4C.crha.bewell.ca> > Content-Type: text/plain; charset="us-ascii" > > I have a question regarding scheduled maintenance of rotary microtomes. I > run a simulation lab for students of lab technology. Part of their > histology rotation involves learning how to produce acceptable sections on > the microtome. I currently have 3 Leica rotary (manual) microtomes, which > are used by students a total of 18-20 days per year (for educational > purposes, not to produce diagnostic materials). In addition, I may use a > microtome for a couple weeks total each year to produce control materials > and to troubleshoot staining issues. > > Currently, the microtomes are serviced by a Leica-educated professional > annually for preventative maintenance. As these microtomes are only used > infrequently, is this level of maintenance necessary (or recommended)? I am > contemplating switching to a bi-annual schedule as a cost-saving measure. > > Does anyone have any advice or experience regarding maintaining microtomes > in a low-volume setting? > > Cheers, > > Allen > > > > ________________________________ > This message and any attached documents are only for the use of the > intended recipient(s), are confidential and may contain privileged > information. Any unauthorized review, use, retransmission, or other > disclosure is strictly prohibited. If you have received this message in > error, please notify the sender immediately, and then delete the original > message. Thank you. > > > ------------------------------ > > Message: 2 > Date: Thu, 27 Dec 2012 19:04:29 +0000 > From: "Koehnlein, Melissa" > Subject: [Histonet] Melanin Bleaching > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <87BF46BACB9B124EAD75F536E82A9BEDE0B4D2@CAMPUSMB5.usask.ca> > Content-Type: text/plain; charset=us-ascii > > Melanin can be distinguished from DAB by counterstaining with Azure B, > which gives a dark green color to the melanin. We've used the procedure > below with success: > > Immunoperoxidase technique modified by counterstain with azure B as a > diagnostic aid in evaluating heavily pigmented melanocytic neoplasms: > > http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0560.1991.tb01381.x/abstract > > Melissa Koehnlein > Lab Technician > Prairie Diagnostic Services Inc. > 52 Campus Drive, Saskatoon, SK S7N 5B4 > Phone (306) 966-7223 > Fax (306) 966-2488 > Email mailto:melissa.koehnlein@pds.usask.ca > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of > histonet-request@lists.utsouthwestern.edu [ > histonet-request@lists.utsouthwestern.edu] > Sent: Thursday, December 27, 2012 12:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 109, Issue 29 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Melanin Bleaching (Janci Wellborn) > 2. Re: Melanin Bleaching (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 26 Dec 2012 15:39:40 -0600 > From: "Janci Wellborn" > Subject: [Histonet] Melanin Bleaching > To: > Message-ID: <50DB1A3C020000B2000E79E3@upbcgwg06> > Content-Type: text/plain; charset="us-ascii" > > I am looking for alternatives to the potassium permanganate melanin > bleaching procedure when doing IHC. I have seen literature references to > doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa > counterstain in the past. Does any one have either procedure that they > would be willing to share? > > We are a small volume lab and the costs associated with bringing in a > different color IHC kit is not justified. > > Any help would be greatly appreciated. > > Janci > > > > Janci R Wellborn, HTL (ASCP) > Laboratory Technical Coordinator > Histology > Alton Memorial Hospital > 1 Memorial Drive > Alton, Ill 62002 > 618-463-7082 > > ------------------------------ > > Message: 2 > Date: Wed, 26 Dec 2012 13:47:23 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Melanin Bleaching > To: Janci Wellborn , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1356558443.97578.YahooMailNeo@web163106.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Melanin bleaching has to be done prior to the IHC procedure and the most > reliable?way is using potassium permanganate. Any other option will produce > less than optimal results. > Ren? J. > > From: Janci Wellborn > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, December 26, 2012 4:39 PM > Subject: [Histonet] Melanin Bleaching > > I am looking for alternatives to the potassium permanganate melanin > bleaching procedure when doing IHC. I have seen literature references to > doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa > counterstain in the past. Does any one have either procedure that they > would be willing to share? > > We are a small volume lab and the costs associated with bringing in a > different color IHC kit is not justified. > > Any help would be greatly appreciated. > > Janci > > > > Janci R Wellborn, HTL (ASCP) > Laboratory Technical Coordinator > Histology > Alton Memorial Hospital > 1 Memorial Drive > Alton, Ill 62002 > 618-463-7082 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 109, Issue 29 > ***************************************** > > > > ------------------------------ > > Message: 3 > Date: Thu, 27 Dec 2012 19:35:33 +0000 > From: "Morken, Timothy" > Subject: [Histonet] RE: Microtome maintenance frequency > To: "Allen Keeping D." > Cc: Histonet > Message-ID: <761E2B5697F795489C8710BCC72141FF0375BC@ex07.net.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Allen, > > Yes the service contract is very expensive. In fact, at our hospital we > decided to forgo the service contract on microtomes and only call for > service when a microtome has a problem. That has saved us tens of thousands > of dollars over the years. In the four years since we have done that we > have only serviced 3 microtomes for a cost of about $4,000. The annual > contract would have been $64,000 during that period! And in fact, the only > problems we have had were related to electronic ribbon cables breaking from > repeated bending - poor design by the manufacturer. We also have a 7 year > replacement policy so they aren't going to get badly worn like some places > that never replace them. > > The minimal use of your microtomes means they aren't going to have the > wear that we see in the hospital. Your problem will be from oil or grease > drying out and making the operation sticky or slow. > > Be sure you do any suggested daily, weekly or monthly maintenance outlined > in the microtome manual. Also be sure to learn what signs indicate a > problem with the microtome. It would be good to have someone use the > microtome on a regular basis just to keep the mechanics smoothly operating > - maybe once a month in your case. > > An accredited lab simply needs to document the regular maintenance and any > repairs done to satisfy inspectors. > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allen Keeping D. > Sent: Thursday, December 27, 2012 10:40 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Microtome maintenance frequency > > I have a question regarding scheduled maintenance of rotary microtomes. I > run a simulation lab for students of lab technology. Part of their > histology rotation involves learning how to produce acceptable sections on > the microtome. I currently have 3 Leica rotary (manual) microtomes, which > are used by students a total of 18-20 days per year (for educational > purposes, not to produce diagnostic materials). In addition, I may use a > microtome for a couple weeks total each year to produce control materials > and to troubleshoot staining issues. > > Currently, the microtomes are serviced by a Leica-educated professional > annually for preventative maintenance. As these microtomes are only used > infrequently, is this level of maintenance necessary (or recommended)? I am > contemplating switching to a bi-annual schedule as a cost-saving measure. > > Does anyone have any advice or experience regarding maintaining microtomes > in a low-volume setting? > > Cheers, > > Allen > > > > ________________________________ > This message and any attached documents are only for the use of the > intended recipient(s), are confidential and may contain privileged > information. Any unauthorized review, use, retransmission, or other > disclosure is strictly prohibited. If you have received this message in > error, please notify the sender immediately, and then delete the original > message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Thu, 27 Dec 2012 13:15:55 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Microtome maintenance frequency > To: "Allen Keeping D." , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <1356642955.99063.YahooMailNeo@web163102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > For your usage level it is unnecessary an annual maintenance. As a matter > of fact I am sure you will be able to use those Leica microtomes for many > years ar the same usage level without needing any "capital" maintenance. > > Ren? J. > > From: Allen Keeping D. > To: "'histonet@lists.utsouthwestern.edu'" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, December 27, 2012 1:40 PM > Subject: [Histonet] Microtome maintenance frequency > > I have a question regarding scheduled maintenance of rotary microtomes. I > run a simulation lab for students of lab technology. Part of their > histology rotation involves learning how to produce acceptable sections on > the microtome. I currently have 3 Leica rotary (manual) microtomes, which > are used by students a total of 18-20 days per year (for educational > purposes, not to produce diagnostic materials). In addition, I may use a > microtome for a couple weeks total each year to produce control materials > and to troubleshoot staining issues. > > Currently, the microtomes are serviced by a Leica-educated professional > annually for preventative maintenance. As these microtomes are only used > infrequently, is this level of maintenance necessary (or recommended)? I am > contemplating switching to a bi-ann From mhanna <@t> histosearch.com Fri Dec 28 13:28:16 2012 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Fri Dec 28 13:30:07 2012 Subject: [Histonet] Help In-Reply-To: References: Message-ID: <50DDF2D0.2030805@histosearch.com> Hi Ly, When you click on the "Unsubscribe" button, an email is sent to you to confirm you want to unsubscribe. It has a link to click to confirm you want to unsubscribe. Is the confirmation email getting lost in your junk folder? You won't be unsubscribed until you click the link in the confirmation email. The confirmation email should have a subject like, "confirm 1a5b...." and a return address of "histonet-request@list.utsouthwestern.edu". Hope this helps. Best Regards, Marvin Hanna On 12/28/2012 2:04 PM, Ly Nguyen wrote: > I want to be remove from the list. I've tried the unsubscribe link be low > several time but it seem like it doesn't work because I'm still receiving > daily email > > From jqb7 <@t> cdc.gov Fri Dec 28 13:33:25 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Dec 28 13:34:49 2012 Subject: [Histonet] Help In-Reply-To: <50DDF2D0.2030805@histosearch.com> References: <50DDF2D0.2030805@histosearch.com> Message-ID: For some reason I just received 3 emails from Histonet asking me to confirm if I want to be unsubscribed....I did not request to be unsubscribed. Did anyone else receive emails like this? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marvin Hanna Sent: Friday, December 28, 2012 2:28 PM To: histonet@lists.utsouthwestern.edu; ln0517@gmail.com Subject: Re: [Histonet] Help Hi Ly, When you click on the "Unsubscribe" button, an email is sent to you to confirm you want to unsubscribe. It has a link to click to confirm you want to unsubscribe. Is the confirmation email getting lost in your junk folder? You won't be unsubscribed until you click the link in the confirmation email. The confirmation email should have a subject like, "confirm 1a5b...." and a return address of "histonet-request@list.utsouthwestern.edu". Hope this helps. Best Regards, Marvin Hanna On 12/28/2012 2:04 PM, Ly Nguyen wrote: > I want to be remove from the list. I've tried the unsubscribe link be > low several time but it seem like it doesn't work because I'm still > receiving daily email > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsjurczak <@t> comcast.net Fri Dec 28 14:18:39 2012 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Fri Dec 28 14:18:57 2012 Subject: [Histonet] CAP and negative IHC controls Message-ID: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? From mhanna <@t> histosearch.com Fri Dec 28 14:26:56 2012 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Fri Dec 28 14:27:24 2012 Subject: [Histonet] Help In-Reply-To: References: <50DDF2D0.2030805@histosearch.com> Message-ID: <50DE0090.1050803@histosearch.com> Hi Jeanine, Hmmm... The only way that should happen is if someone put your email address in and clicked "unsubscribe" 3 times. That's the purpose of the confirmation email so that no one else can unsubscribe (or subscribe) you. So, anyone out there trying to unsubscribe Jeanine, please stop. The computer server is smarter than you. And we like Jeanine. :-) Marvin On 12/28/2012 2:33 PM, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: > For some reason I just received 3 emails from Histonet asking me to confirm if I want to be unsubscribed....I did not request to be unsubscribed. > > Did anyone else receive emails like this? > > Jeanine H. Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 404-639-3590 > jeanine.bartlett@cdc.hhs.gov > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marvin Hanna > Sent: Friday, December 28, 2012 2:28 PM > To: histonet@lists.utsouthwestern.edu; ln0517@gmail.com > Subject: Re: [Histonet] Help > > Hi Ly, > > When you click on the "Unsubscribe" button, an email is sent to you to confirm you want to unsubscribe. It has a link to click to confirm you want to unsubscribe. Is the confirmation email getting lost in your junk folder? You won't be unsubscribed until you click the link in the confirmation email. The confirmation email should have a subject like, "confirm 1a5b...." and a return address of "histonet-request@list.utsouthwestern.edu". Hope this helps. > > Best Regards, > > Marvin Hanna > > On 12/28/2012 2:04 PM, Ly Nguyen wrote: >> I want to be remove from the list. I've tried the unsubscribe link be >> low several time but it seem like it doesn't work because I'm still >> receiving daily email >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DSiena <@t> statlab.com Fri Dec 28 14:41:22 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Fri Dec 28 14:41:28 2012 Subject: [Histonet] CAP and negative IHC controls In-Reply-To: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: It is my understanding that you still need a negative tissue control but if using a polymer detection kit that you may stop doing a negative reagent control. The negative tissue control is a tissue known to not contain the antigen in the tissue, it can be an internal control, or a separate control but if internal control, then it must be designated as to how it will be used in your procedure manual. Hope that helps. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net Sent: Friday, December 28, 2012 2:19 PM To: . Subject: [Histonet] CAP and negative IHC controls How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Dec 28 14:53:20 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Dec 28 14:53:33 2012 Subject: [Histonet] CAP and negative IHC controls In-Reply-To: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <50DDC070.7400.0077.1@harthosp.org> That decision needs to be made by your Medical Director. In my laboratory we use polymer detection for almost all of our IHCs and, therefore, I don't require a "Negative Reagent Control" for those cases. We have one antibody that requires avidin-biotin detection and we run a negative reagent control in parallel whenever we run that antibody. In my experience, if you are using polymer detection and your antibodies are properly optimized and validated, the negative reagent control slide serves no useful purpose and, therefore, is not needed. Best wishes to everyone on Histonet for the coming New Year? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> 12/28/2012 3:18 PM >>> How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Dec 28 15:03:04 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Dec 28 15:03:15 2012 Subject: [Histonet] CAP and negative IHC controls Message-ID: <50DDC2B8.7400.0077.1@harthosp.org> That decision needs to be made by your Medical Director. In my laboratory we use polymer detection for almost all of our IHCs and, therefore, I don't require a "Negative Reagent Control" for those cases. We have one antibody that requires avidin-biotin detection and we run a negative reagent control in parallel whenever we run that antibody. In my experience, if you are using polymer detection and your antibodies are properly optimized and validated, the negative reagent control slide serves no useful purpose and, therefore, is not needed. Best wishes to everyone on Histonet for the coming New Year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> 12/28/2012 3:18 PM >>> How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Fri Dec 28 21:10:08 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Dec 28 21:10:12 2012 Subject: [Histonet] Re: Histonet Digest, Vol 109, Issue 30 In-Reply-To: <50ddde5e.02a4b60a.665d.1a18SMTPIN_ADDED_MISSING@mx.google.com> References: <50ddde5e.02a4b60a.665d.1a18SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Kimura, The answer to your question is YES. Both Tween 20 & Triton 100 are non-ionic detergents. Tween 20 is not as strong/effective as Triton 100, but you can use higher percentage Tw20 or prolong the permeabilization timing. Best! Madeleine Huey, BS, MT, HTL & QIHC (ASCP) madeleine_h@elcaminohospital.org On Fri, Dec 28, 2012 at 10:01 AM, wrote: > Subject: [Histonet] In permeabilization, can we use Tween-20 instead > of Triton-100? > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Dear Histonetters > > Hi. > Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL). > The primary antibody is for detecting some intracellular > antigen(antigen in cytoplasm). > So I think permeabilization process may be necessary after fixation by 4% PFA. > Does anyone know whether we can use Tween-20 in place of Triton-100 > for permeabilization? > And can we use same protocol in immunostaining of BAL cells as in > immunostaining for cell line? > > Thanks in advance. > > H Kimura From badzrosari <@t> yahoo.com Sun Dec 30 09:56:05 2012 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Sun Dec 30 09:56:09 2012 Subject: [Histonet] vacuum sealer Message-ID: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> Hi histonets..Im looking for a good brand of vacuum sealers for storing histology tissue samples.Dont you have any issues with this sealers.Im looking for one which retains a little formalin in it.I want to know which type you are using..Thanks a lot.Have a great day.. From wdesalvo.cac <@t> outlook.com Sun Dec 30 12:06:02 2012 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Sun Dec 30 12:06:07 2012 Subject: [Histonet] vacuum sealer In-Reply-To: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> References: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> Message-ID: Cardinal Health - Kapac System, 18 inch sealer w/ special bags for storing formalin. Best system for Histo William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Sun, 30 Dec 2012 07:56:05 -0800 > From: badzrosari@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] vacuum sealer > > Hi histonets..Im looking for a good brand of vacuum sealers for storing histology tissue samples.Dont you have any issues with this sealers.Im looking for one which retains a little formalin in it.I want to know which type you are using..Thanks a lot.Have a great day.. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Dec 30 12:35:15 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Dec 30 12:35:27 2012 Subject: [Histonet] Argene Inc. Message-ID: <50E04313.7400.0077.1@harthosp.org> Does anyone know if Argene/Biosoft (France) still exists and, if so, do they still sell products here in the USA? They had a number of interesting infectious disease monoclonal antibodies. Thank you! Best wishes to everyone for 2013! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax From ibernard <@t> uab.edu Sun Dec 30 21:01:52 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Dec 30 21:01:56 2012 Subject: [Histonet] CAP New requirement: Cytopathology Exclusions from Submissions In-Reply-To: References: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> Message-ID: Fellow Laboratorians who process Cytopathology specimens, specifically, Non-Gyn specimens. A policy that list specimens that are excluded from routine submission to cytology for processing. Please share your facilities' approved examples on the types of specimens, and a justification and reference as to why this should be excluded. Thanks Ian Colorado From ibernard <@t> uab.edu Sun Dec 30 21:17:42 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Dec 30 21:17:46 2012 Subject: [Histonet] CAP New requirement: Cytopathology Exclusions from Submissions In-Reply-To: <5A15C72B-C3E3-41E2-B395-51BA870B7A4D@gmail.com> References: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> <5A15C72B-C3E3-41E2-B395-51BA870B7A4D@gmail.com> Message-ID: Yes, This is a new checklist item as of 07/11/2011. Cytopathology Exclusion question: CYP.01650. Ian -----Original Message----- From: Oscar [mailto:omgwakemed@gmail.com] Sent: Sunday, December 30, 2012 9:15 PM To: Ian R Bernard Subject: Re: [Histonet] CAP New requirement: Cytopathology Exclusions from Submissions Hi Neha. I was checking the Histonet, and found the question below. Is there a requirement for non-GYN specimens? There is one for Histo, but didn't know there was a Cyto....... Sent from my iPad2 On Dec 30, 2012, at 10:01 PM, Ian R Bernard wrote: > Fellow Laboratorians who process Cytopathology specimens, specifically, Non-Gyn specimens. A policy that list specimens that are excluded from routine submission to cytology for processing. > > Please share your facilities' approved examples on the types of specimens, and a justification and reference as to why this should be excluded. > > Thanks > Ian > Colorado > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Sun Dec 30 22:16:31 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Dec 30 22:16:39 2012 Subject: [Histonet] FNA procedure in Cytopathology Message-ID: With HIPPA privacy rules, patient identifies should be carefully considered. For the "time out" procedure for patient safety, during the FNA process ,we ask the patient to provide DOB and name only. For the slide labeling part of the process, what two patient identifiers do you all annotate on the patient slides. The same aforementioned or something else. The impasse is space on the slide. is patient first initial of last name and last four acceptable? Or should we stick with name and DOB for consistency of policy. Or does it matter? Ian From sdysart <@t> mirnarx.com Mon Dec 31 08:31:15 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Dec 31 08:31:29 2012 Subject: [Histonet] vacuum sealer In-Reply-To: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> References: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5047B2D3D4@BL2PRD0710MB363.namprd07.prod.outlook.com> Get one from Walmart. Half the price and last just as long =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario Sent: Sunday, December 30, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] vacuum sealer Hi histonets..Im looking for a good brand of vacuum sealers for storing histology tissue samples.Dont you have any issues with this sealers.Im looking for one which retains a little formalin in it.I want to know which type you are using..Thanks a lot.Have a great day.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Mon Dec 31 14:39:27 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Dec 31 14:39:35 2012 Subject: [Histonet] vacuum sealer In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5047B2D3D4@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com>, <8A70A9B2ECDD084DACFE6C59FCF86D5047B2D3D4@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56EBF2@EXCHANGEMB1.hmc.hurleymc.com> The only vacuum system I have experience with is the Kapac vacuum bag system. There are various bag sizes to choose from, and the bags themselves are quite thick. I must also put out there, that after sealing, even doing it twice (two seals) on each bag, and storing, we have experienced leakage after a few years. When going back through for either retrieval or discard, we run across ~10% that have leaked out and the tissue has dried up. :( Would be interested in seeing if there is a product out there that is better that I missed out on! Happy New Year everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sarah Dysart [sdysart@mirnarx.com] Sent: Monday, December 31, 2012 9:31 AM To: Bernadette del Rosario; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] vacuum sealer Get one from Walmart. Half the price and last just as long =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario Sent: Sunday, December 30, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] vacuum sealer Hi histonets..Im looking for a good brand of vacuum sealers for storing histology tissue samples.Dont you have any issues with this sealers.Im looking for one which retains a little formalin in it.I want to know which type you are using..Thanks a lot.Have a great day.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.Hart <@t> bms.com Mon Dec 31 15:04:29 2012 From: Heather.Hart <@t> bms.com (Hart, Heather) Date: Mon Dec 31 15:04:12 2012 Subject: [Histonet] vacuum sealer In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56EBF2@EXCHANGEMB1.hmc.hurleymc.com> References: <1356882965.47997.YahooMailNeo@web121504.mail.ne1.yahoo.com>, <8A70A9B2ECDD084DACFE6C59FCF86D5047B2D3D4@BL2PRD0710MB363.namprd07.prod.outlook.com> <89F4666A496DC949A819ECC40E11C867BF56EBF2@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <55EA897EF9AC2342BD9322B86AC195B6171E47B134@ushpwbmsmmp007.one.ads.bms.com> Sipromac table top vacuum sealer - we have the 350 model. http://www.sipromac.com/a_p01a.html >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich >Sent: Monday, December 31, 2012 2:39 PM >To: Sarah Dysart; Bernadette del Rosario; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] vacuum sealer > >The only vacuum system I have experience with is the Kapac vacuum bag >system. There are various bag sizes to choose from, and the bags >themselves are quite thick. >I must also put out there, that after sealing, even doing it twice (two >seals) on each bag, and storing, we have experienced leakage after a few >years. When going back through for either retrieval or discard, we run >across ~10% that have leaked out and the tissue has dried up. :( >Would be interested in seeing if there is a product out there that is >better that I missed out on! > >Happy New Year everyone!! > >Lynette > >Lynette Pavelich, HT(ASCP) >Histology Supervisor >Hurley Medical Center >One Hurley Plaza >Flint, MI 48503 > >ph: 810.262.9948 >mobile: 810.444.7966 > >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu [histonet- >bounces@lists.utsouthwestern.edu] on behalf of Sarah Dysart >[sdysart@mirnarx.com] >Sent: Monday, December 31, 2012 9:31 AM >To: Bernadette del Rosario; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] vacuum sealer > >Get one from Walmart. Half the price and last just as long =) > >Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) >Histotechnologist >Mirna Therapeutics >2150 Woodward Street >Suite 100 >Austin, Texas 78744 >(512)901-0900 ext. 6912 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario >Sent: Sunday, December 30, 2012 9:56 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] vacuum sealer > >Hi histonets..Im looking for a good brand of vacuum sealers for storing >histology tissue samples.Dont you have any issues with this sealers.Im >looking for one which retains a little formalin in it.I want to know >which type you are using..Thanks a lot.Have a great day.. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. 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