From gu.lang <@t> gmx.at Wed Aug 1 02:30:54 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Aug 1 02:31:11 2012 Subject: [Histonet] symphony another question Message-ID: <001301cd6fb7$96043990$c20cacb0$@gmx.at> Hi! A question for Symphony-users. Do you enjoy working with the Symphony? Is it really a change to the better in comparison to other combined staining-coverslipping devices? I don't ask for quality or patient security - just for user concerns. Did it ever happen, that a run-error turned the system down (like it sometimes does with our Ultra)? What are the consequences? Just curious. Gudrun From gu.lang <@t> gmx.at Wed Aug 1 02:34:39 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Aug 1 02:35:05 2012 Subject: AW: [Histonet] (no subject) In-Reply-To: References: <1343758982.12170.YahooMailNeo@web5716.biz.mail.ne1.yahoo.com> Message-ID: <001a01cd6fb8$1c29cee0$547d6ca0$@gmx.at> ... thinking about muscle packed little woman in labcoat tearing one phone book after another ... : ) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rena Fail Gesendet: Dienstag, 31. Juli 2012 23:33 An: Paula Pierce Cc: Histonet Betreff: Re: [Histonet] (no subject) Can't beat phone books. Cheap, torn in half they are wider than a kimwipe, you're recycling, and providing therapy in the form of stress relief obtained tearing up phone books. Rena On Tue, Jul 31, 2012 at 2:23 PM, Paula Pierce < contact@excaliburpathology.com> wrote: > Greetings, > > does anyone know of a less expensive substitute for Kim-wipes? > > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 8901 S. Santa Fe, Suite G > Oklahoma City, OK 73139 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> texasheart.org Wed Aug 1 05:57:16 2012 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Wed Aug 1 06:01:05 2012 Subject: [Histonet] calcium buildup Message-ID: Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. From daniel.afrika <@t> nioh.nhls.ac.za Wed Aug 1 06:38:13 2012 From: daniel.afrika <@t> nioh.nhls.ac.za (Daniel Afrika) Date: Wed Aug 1 06:42:16 2012 Subject: [Histonet] calcium buildup In-Reply-To: References: Message-ID: Hi Molinari I used microbiology long cotton wool swabs. If they are short extend them by burning the plastic ends and joining them while they are hot. Make sure the join is straight. Unscrew the nozzles from the machine and push the swabs in and out to clean and open the holes. If the blockage is hard u may use any small sharp metal pins to unblock the holes. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 01 August 2012 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] calcium buildup Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. From TGoins <@t> mt.gov Wed Aug 1 08:59:36 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Aug 1 08:59:49 2012 Subject: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining In-Reply-To: <28587D1EA667F84BBAF99926FE94F5F40108C453@livma07.intra.swsahs.nsw.gov.au> References: <28587D1EA667F84BBAF99926FE94F5F40108C453@livma07.intra.swsahs.nsw.gov.au> Message-ID: The proteolytic activity of the enzymes can be non-specific (Pronase) or specific (peptide bonds at lysine and arginine residues, Trypsin). The effect of formalin fixation is not identified, so we always assess Pepsin, Trypsin and Pronase along with heat retrieval methods regardless of what the supplier data sheet recommends. Tresa Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) Young.Kwun@sswahs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Wed Aug 1 09:23:39 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Aug 1 09:23:46 2012 Subject: [Histonet] New Position - IHC Reagent and Antibody Sales Message-ID: <7852a3253d27e281dc604359c034efc1@mail.gmail.com> Good Morning Histonet, Our client has recently opened a new expansion sales opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in Histology and IHC has recently opened a new expansion sales opportunity in the Midwest Region to focus on driving IHC Reagent and Antibody sales. We are searching for a histology professional who has a strong background in IHC and antibodies that would be interested in working in a team sales environment. Though sales experience is a plus, this position does not require previous sales experience and this would be outstanding opportunity for someone looking to break into sales that has a strong histology background. The ideal location for the position would be in the greater Minneapolis area, however we will consider candidates in the region (MN, ND, SD, IA, NE, KS). The position offer s strong package including a base salary, competitive commission structure, car allowance, gas card, cell phone, laptop computer, and full benefits. If you or anyone you may know would be interested in the position, please contact me directly at mw@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From boneimage8 <@t> gmail.com Wed Aug 1 10:24:16 2012 From: boneimage8 <@t> gmail.com (Marc DeCarlo) Date: Wed Aug 1 10:24:23 2012 Subject: [Histonet] 50mm x 100mm staining rack available Message-ID: Hi histoland, Thanks for all the help a few weeks ago. I found a commercially available 15 place staining rack for 50mm x 100mm slides. It works perfectly and I recommend it to anyone needing to stain slides of that size. It's a bit difficult to find on the web, and since I don't believe in blatant advertising message me if you are interested and I will send you the link. Cheers! Marc DeCarlo From rsrichmond <@t> gmail.com Wed Aug 1 10:42:59 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Aug 1 10:43:05 2012 Subject: [Histonet] Re: Expert Level Cutting and Embedding Message-ID: I see Jay Lundgren's point, but it seems to me that the present shortage of histotechnologists makes HistoCare's advertisement of their services entirely appropriate for HistoNet. Bob Richmond Samurai Pathologist Knoxville TN From one_angel_secret <@t> yahoo.com Wed Aug 1 10:45:29 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Aug 1 10:45:38 2012 Subject: [Histonet] RE: Secondary antibody causing nuclear staining In-Reply-To: References: <785BBF0C5F49CE41BA74460A43A08F02322D1B7A48@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: <1343835929.82785.YahooMailNeo@web112303.mail.gq1.yahoo.com> Eva, ??????? Have you gotten your problem resolved yet? I was curious as to what heat method you are using? Are you using a microwave? I'm sorry to be asking so many questions and not getting back to you quickly. I am thinking if you have irregular positive staining in the same Negative control tissue on different days that its a technique issue. Maybe the microwave is heating irregular? Ive also seen when using a microwave that I got better results when I would make sure they dont evaporate when transferring to next reagent?and I circulated the buffer with a pipette in between the heating process.? ? Now?if your using a steamer < some people still do, dont laugh> then perhaps if its the second batch the retreival is hotter than for the first batch causing overstaining for that second batch?. ? Is there any possibliltiy at all some of the slides could be drying out during the staining process? I have seen nuclear artifact in some cases this happened to. ? Are you making up your DAB everyday, there are some DAB's that you have to make up and they get old quickly and dont stain as well later on. ? So many questions, sorry. I'd love to help you if you havnt figured this out yet. Please feel free to email me. ? Kim D ? ? ________________________________ From: Eva Permaul To: "Reynolds, Donna M" ; histonet Sent: Wednesday, July 25, 2012 10:59 AM Subject: Re: [Histonet] RE: Secondary antibody causing nuclear staining I do see positive nuclei in the NC. That is what I am asking about. I know I could switch methods but my question is also why if it is happening is it not as strong all the time? Why are the cells very light one day and dark the next? What is causing them to stain? Just curious is all. Eva On Wed, Jul 25, 2012 at 10:04 AM, Reynolds,Donna M wrote: > > If you are running a negative control (no primary)with your ABC staining > wouldn't you see the same nuclear labeling in the NC ? Thus alerting you to > false staining and indicating that you should try a HRP conjugated > secondary or use a polymer system. > Good discussion thank Tony. > Donna Reynolds HT(ASCP) Chief Histologist IHC Lab > > -----Original Message----- > > I understand the point about the biotin and I should have said that > > when using the ABC method we have taken to always using an > > avidin/biotin blocking kit. We are using biotinylated secondary > > antibodies from Vector. I have seen the same problem occur in our > > anti-mouse, anti-rabbit and anti-goat. In my last run I had stomach > > fundus as well as skin melanoma, both had pos.nuclei in the negative > > (no primary). In another run I had colon ca and breast ca, the breast > > ca had fewer pos. nuclei than the colon ca but they were still there. > > Some days the positive nuclei are stronger in a sample that was just > > weakly positive before. Just want to understand what it is and what > effects it. > > Thank you all for your ideas. > > Eva Permaul > > Georgetown University > > > > On Mon, Jul 23, 2012 at 7:16 PM, Tony Henwood (SCHN) < > > tony.henwood@health.nsw.gov.au> wrote: > > > >> I should have added that this was from the workshop notes on a > >> Hypotheticals Workshop I ran last year at our Australian National > Meeting. > >> > >> Regards > >> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > >> Laboratory Manager & Senior Scientist > >> Tel: 612 9845 3306 > >> Fax: 612 9845 3318 > >> the children's hospital at westmead > >> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > >> Westmead NSW 2145, AUSTRALIA > >> > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: > >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood > >> (SCHN) > >> Sent: Tuesday, 24 July 2012 9:00 AM > >> To: 'Eva Permaul'; histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] Secondary antibody causing nuclear staining? > >> > >> It is possible that this is due to "Biotin nuclei" where excess > >> biotin is found in the nuclei of some cells, see below: > >> > >> Optically clear nuclei have been reported in endometrial epithelium > >> associated with first and second trimester abortions (Sickel & di > >> Sant'Agnese 1994). Optically clear nuclei have also been found in > >> different types of tissues of diverse organs such as ovary, thyroid > >> and lung (Nakatani et al 1994, Mount & Cooper 2001). The optically > >> clear nuclei contain excess biotin. > >> > >> Endogenous biotin immunoreactivity is generally not visualized in > >> formalin fixed, paraffin-embedded tissues unless a heat-induced > >> antigen retrieval step has been introduced (Mount & Cooper 2001). > >> > >> In this placental section, optically clear nuclei (containing biotin) > >> bind to the streptavidin of the ABC technique giving a reaction > >> similar to that seen with CMV containing cells. If a polymer method > >> (or even the original Sternberger's PAP method) is used then this > >> anomalous staining will disappear, thus allowing confident > demonstration of CMV infected nuclei. > >> > >> The false-positive staining pattern caused by endogenous biotin can > >> be cytoplasmic or nuclear. A report of positive immunoreactivity of > >> hepatocellular carcinomas for inhibin was later determined to be a > >> false-positive finding due to cytoplasmic endogenous biotin. Steroid > >> cell tumours of the ovary were found to demonstrate endogenous biotin > >> cytoplasmic staining in 36% of cases. Immunoreactivity for > >> anti-Herpes virus immunohistochemical staining in a series of > >> endometria was also later determined to be a false-positive result > >> due to biotin. The prominent intranuclear inclusions, resembling > >> herpes virus cytopathic effect, were caused by intranuclear biotin > >> and not viral particles. Similar false positive staining for CMV in > >> products of conception has also been reported (Mount & Cooper 2001). > >> > >> False-positive staining can be cytoplasmic or nuclear. When > >> cytoplasmic, the appearance of the false signal is that of a dull > >> brown granular or fluffy staining pattern. If this quality of > >> staining is observed with several different antibodies, endogenous > >> staining by biotin should be considered. When nuclear, a > >> false-positive reaction may be associated with optically clear nuclei > >> identified on H&E stained sections. False-positive staining due to > >> endogenous biotin, however, does not occur in a cell membrane pattern > (Mount & Cooper 2001). > >> > >> Mount SL & Cooper K (2001) "Beware of biotin: a source of > >> false-positive immunohistochemistry" Current Diagnostic Pathology >? 7:161-167. > >> Nakatani et al (1994) Am J Surg Pathol 18(6):637-642. > >> Sickel & di Sant'Agnese (1994) Arch Pathol Lab Med 118:831-833 > >> > >> > >> Regards > >> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > >> Laboratory Manager & Senior Scientist > >> Tel: 612 9845 3306 > >> Fax: 612 9845 3318 > >> the children's hospital at westmead > >> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > >> Westmead NSW 2145, AUSTRALIA > >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Aug 1 11:27:37 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Aug 1 11:27:41 2012 Subject: AW: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining In-Reply-To: References: <28587D1EA667F84BBAF99926FE94F5F40108C453@livma07.intra.swsahs.nsw.gov.au> Message-ID: <004401cd7002$906c3af0$b144b0d0$@gmx.at> I think formalin fixation does something similar to enzyme-"binding" as to antibody-binding, if the enzyme has a specific binding site. The aminoacids are masked by methylol-adducts and therefore the protein is protected against eg pepsin. So using combinated HIER and PIER is not only summarizing the effects. HIER renders PIER more effective and therefore more aggressive. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Goins, Tresa Gesendet: Mittwoch, 01. August 2012 16:00 An: Young Kwun; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining The proteolytic activity of the enzymes can be non-specific (Pronase) or specific (peptide bonds at lysine and arginine residues, Trypsin). The effect of formalin fixation is not identified, so we always assess Pepsin, Trypsin and Pronase along with heat retrieval methods regardless of what the supplier data sheet recommends. Tresa Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) Young.Kwun@sswahs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Wed Aug 1 11:50:14 2012 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Aug 1 11:51:40 2012 Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Message-ID: Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana From wbenton <@t> cua.md Wed Aug 1 11:55:19 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Aug 1 11:55:51 2012 Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD92CCB563D07@CUAEXH1.GCU-MD.local> If I recall correctly Newcomer Supply and Poly Scientific both sell NFR. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmccaig@ckha.on.ca] Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TGoins <@t> mt.gov Wed Aug 1 12:03:55 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Aug 1 12:04:05 2012 Subject: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining In-Reply-To: <004401cd7002$906c3af0$b144b0d0$@gmx.at> References: <28587D1EA667F84BBAF99926FE94F5F40108C453@livma07.intra.swsahs.nsw.gov.au> <004401cd7002$906c3af0$b144b0d0$@gmx.at> Message-ID: When re-reading my e-mail, it does sound like we use heat and enzymatic retrieval together - we do not - we assess the effective of all retrieval methods used singly. Tresa -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Wednesday, August 01, 2012 10:28 AM To: Goins, Tresa Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining I think formalin fixation does something similar to enzyme-"binding" as to antibody-binding, if the enzyme has a specific binding site. The aminoacids are masked by methylol-adducts and therefore the protein is protected against eg pepsin. So using combinated HIER and PIER is not only summarizing the effects. HIER renders PIER more effective and therefore more aggressive. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Goins, Tresa Gesendet: Mittwoch, 01. August 2012 16:00 An: Young Kwun; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining The proteolytic activity of the enzymes can be non-specific (Pronase) or specific (peptide bonds at lysine and arginine residues, Trypsin). The effect of formalin fixation is not identified, so we always assess Pepsin, Trypsin and Pronase along with heat retrieval methods regardless of what the supplier data sheet recommends. Tresa Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) Young.Kwun@sswahs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Wed Aug 1 12:14:27 2012 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Wed Aug 1 12:14:33 2012 Subject: [Histonet] MOHS Tech Message-ID: <53FC421CC200C5429929EDE6C3676F300206C9B8@msgebe34> Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org From contact <@t> histocare.com Wed Aug 1 12:23:42 2012 From: contact <@t> histocare.com (Contact HistoCare) Date: Wed Aug 1 12:23:49 2012 Subject: [Histonet] Expert Level Cutting and Embedding Message-ID: <41DE0C8A-DF30-4979-9684-1DD25C4F46AD@histocare.com> Thanks for all the inquiries! And thanks Mr. Richmond! This is to provide clarity in response to some of the questions asked about HistoCare's service. We provide an experienced technician directly to your lab when a need arises such as when a regular employee has a planned absence like a pregnancy or surgery, or if you have a sudden change in staff from someone quitting or jury duty for example. You don't have to scramble to hurry up and hire the wrong person just to keep the lab running smoothly. The benefit is you get a strong, detailed, and reliable person to embed and cut and minimize the stress and workload on other FTEs. Also there is no long term commitment should your departmental needs change. In the interim you are not taking on additional stress from trying to figure out how to get the work done and you get to take your time to find the right replacement or wait until your regular employee returns. It is not our intent to offend any subscribers to histonet by posting our service. We are available on short notice, flexible with time and can work together on a budget that meets your needs. Please send inquiries and Technician requests to contact@histocare.com. Thanks, Betty Smith, HistoCare Assistant From wbenton <@t> cua.md Wed Aug 1 12:25:24 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Aug 1 12:26:20 2012 Subject: [Histonet] RE: MOHS Tech In-Reply-To: <53FC421CC200C5429929EDE6C3676F300206C9B8@msgebe34> References: <53FC421CC200C5429929EDE6C3676F300206C9B8@msgebe34> Message-ID: <0B8979A204680A42B93A52B486088CD92CCB563D0A@CUAEXH1.GCU-MD.local> I would review the CLIA guidelines, which dictate minimum qualifications to perform certain tasks. http://wwwn.cdc.gov/clia/regs/subpart_m.aspx Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. [Bauer.Karen@mayo.edu] Sent: Wednesday, August 01, 2012 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MOHS Tech Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From vperez <@t> pathreflab.com Wed Aug 1 12:25:08 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Wed Aug 1 12:30:39 2012 Subject: [Histonet] RE: MOHS Tech In-Reply-To: <53FC421CC200C5429929EDE6C3676F300206C9B8@msgebe34> References: <53FC421CC200C5429929EDE6C3676F300206C9B8@msgebe34> Message-ID: For grossing all you need are enough college hours in bio and chem. As to CLIA standards and that's what CAP follows if im correct. They do not have to be a certified histotech to gross tissue. Unless its required by state regulations. Requirements are 6 hours of biology and 6 hours of chemistry and then combination of 12 more hours in bio/chem. Total of 24 hours college credit Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Wednesday, August 01, 2012 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MOHS Tech Importance: Low Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Aug 1 13:00:12 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 1 13:00:17 2012 Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) In-Reply-To: <0B8979A204680A42B93A52B486088CD92CCB563D07@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD92CCB563D07@CUAEXH1.GCU-MD.local> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E288AA0@evcspmbx3.ads.northwestern.edu> As does Rowley Biochemical. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, August 01, 2012 11:55 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) If I recall correctly Newcomer Supply and Poly Scientific both sell NFR. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmccaig@ckha.on.ca] Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Wed Aug 1 13:02:34 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Aug 1 13:02:47 2012 Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) In-Reply-To: References: Message-ID: Hi Diana, I have heard of using Safranin O as a red counterstain and StatLab sells both that and Nuclear Fast Red. Thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, August 01, 2012 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Aug 1 13:18:42 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Aug 1 13:18:49 2012 Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) In-Reply-To: References: Message-ID: The time and trouble of making up a solution of aluminum mordanted nuclear fast red repays you well. The staining solution contains to work well for an entire year. If you must have something else, brazalum, made by substituting brazilin for hematoxylin in Mayer's hemalum, works very well. The solution keeps its staining qualities for 2 or 3 months. Anatech sells brazalum ready-made as "Brazilliant." Although Orth's lithium carmine gives beautiful results, I don't recommend it: it's a pain to make and keeps its staining power for only 2 weeks. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Wed Aug 1 13:46:11 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Aug 1 13:46:20 2012 Subject: [Histonet] New Position - Manager, Workflow Consulting Message-ID: <52049bb49e32e8981a3e0cc34028861c@mail.gmail.com> Good Afternoon Histonet, We have had a new position open with a global leader in histology. Please contact me directly at mw@personifysearch.com to learn more. *Manager, Workflow Consulting* *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Cancer Diagnostics, Anatomical Pathology, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for an Manager, Workflow Consulting in Clinical Diagnostics based in Chicago, IL and covers the Western Part of the US (Chicago West). The person who fills this position can live anywhere in the Western US. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: Commensurate with Experience *Primary Responsibilities:* The primary responsibility of this role will be to achieve Company sales and profitability goals by offering a value-added service to end-user that provides workflow consulting services and lean principles to optimize customer laboratories performance. Drive change in anatomic pathology laboratories utilizing Lean principles, information management, and hardware/software solutions. The solution for such a change, efficiency gains and waste elimination is the Company product offering. Additional Responsibilities: - Achieve monthly, quarterly, annual unit and revenue goals for the Division. Track KPI to measure revenue generated through Lean Consulting Services - Analyze new and existing customer laboratory organizations and workflow practices and recommend short and long-term improvements - Utilize Company Business System tools to credibly recommend changes to lab practices to eliminate waste, reduce cost and improve quality and turnaround times - Analyze and report market trends and innovative competitive activities for Lean Services - Working in conjunction with local sales representatives, Area Sales Managers and Directors of Sales plan and schedule face-face account calls to current and potential end-users. Train Sales force on basic lean principles for them to help market lean services and be able to follow-up with customers - In conjunction with Sales and Marketing, identify and develop key accounts in the territory. In conjunction with Director of Corporate Accounts, manage national accounts within territory requiring corporate coordination to enable closure and compliance of contracts - Prepare monthly territory status reports on lean activities to Management Maintain and report monthly on Workflow opportunities and projects - Manage operating expenses within assigned budget - Maintain technical, product, applications and sales skill knowledge. Maintain current knowledge of competition and market through study of competitive marketing information, competitive literature, and field surveillance or competition - Prospect for all product opportunities. Follow-up on all sales leads with status review immediately upon receipt - Participate in sales meetings and national trade shows as appropriate and authorized - Conduct Lunch and Learns, workshops, seminars or focus groups at local technical society meetings as appropriate and authorized - Promote the Company as the pathology market leader in quality and innovation *Education and Experience Required:* - BA/BS in Life Sciences or equivalent required - MBA preferred but not required - 2-5 years Histology/Pathology laboratory experience in clinical , research or industrial setting desirable but not required - Understanding of pathology marketplace with strong technical acumen - 2-5 years knowledge of Company Business Systems, Six Sigma, or Lean Principles required selling experience or consumables - Outstanding problem solving skills. Can manage multiple layers of personnel within customer site 1-3 years Histology laboratory experience in clinical, research or industrial setting desirable but not required - 1-3 years of product management or sales experience in a related discipline preferred but not required Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From mjdessoye <@t> commonwealthhealth.net Wed Aug 1 13:46:27 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Wed Aug 1 13:46:32 2012 Subject: [Histonet] Cost analysis Message-ID: Hello all, I've been asked to update some dated test cost information. I need to come up with a a total cost (materials, burden [common materials] and labor) for IHC, ISH, special stains, and routine H&E (to include processing, embedding, cutting, staining). I'm not too concerned with IHC and special stains, but I'm hoping to get some input regarding routine H&E and associated work because there's a lot of factors to consider and many are variable. Has anyone done this, and would mind providing some thoughts on this process? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From joelleweaver <@t> hotmail.com Wed Aug 1 13:58:25 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 1 13:58:31 2012 Subject: [Histonet] Cost analysis In-Reply-To: References: Message-ID: QI Macros software or Palisades Decision Tools software may help ( maybe latter for cost-seeking emphasis). It will help you track the data, make good comparision charts,find "hidden" waste and cost, and perform calculations with sensitivity analysis beyond Excel Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 1 Aug 2012 14:46:27 -0400 > From: mjdessoye@commonwealthhealth.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cost analysis > > Hello all, > > I've been asked to update some dated test cost information. I need to > come up with a a total cost (materials, burden [common materials] and > labor) for IHC, ISH, special stains, and routine H&E (to include > processing, embedding, cutting, staining). I'm not too concerned with > IHC and special stains, but I'm hoping to get some input regarding > routine H&E and associated work because there's a lot of factors to > consider and many are variable. Has anyone done this, and would mind > providing some thoughts on this process? > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Aug 1 14:07:24 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Aug 1 14:07:38 2012 Subject: [Histonet] ENFD Testing Message-ID: <003401cd7018$e2dddfd0$a8999f70$@com> Happy Wednesday Histonetters I have had a request from some of our clients to do epidermal nerve fiber density testing. I have found information in how to obtain the specimen but have not found the protocol for processing, cutting, and staining the specimen. To anyone out there who is performing this testing, I would appreciate your insight. I am starting this from scratch. Any information would be welcome. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From azdudley <@t> hotmail.com Wed Aug 1 16:13:09 2012 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Aug 1 16:13:18 2012 Subject: [Histonet] negative controls Message-ID: just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama From tfountain <@t> dsmanitoba.ca Wed Aug 1 16:41:46 2012 From: tfountain <@t> dsmanitoba.ca (Tiana Baskin) Date: Wed Aug 1 16:41:50 2012 Subject: [Histonet] ACTH Antibody Message-ID: Hi I am working with the DAKO ACTH clone 02A3 (concentrate) and I am getting a lot of background staining. I have tried to decreasing the incubation time of the primary and I have tried to increase the dilution however I am starting to loose signal and I still have the background and non-specific staining. I was wondering if anyone has had this experience and whether or not anyone is happy with and/or using the Cellmarque polyclonal ACTH? Thanks :-) -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From LSebree <@t> uwhealth.org Wed Aug 1 16:47:26 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Aug 1 16:47:30 2012 Subject: [Histonet] negative controls In-Reply-To: References: Message-ID: I asked my VMS rep that question and she forwarded it to the higher ups as they had not heard about this yet. Waiting for an answer but even if VMS says we can omit them, our negative aren't all that clean always so don't know if we will or not. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Norm.Burnham <@t> propath.com Wed Aug 1 17:07:46 2012 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Wed Aug 1 17:07:49 2012 Subject: [Histonet] Outstanding Opportunity for a Pathologists' Assistant Message-ID: <82C7248978CB50469FD6BA68EBBEFE670845B251@exchange.propathlab.com> OUTSTANDING OPPORTUNITY FOR A PATHOLOGISTS' ASSISTANT ProPath, a high volume, privately owned pathology practice located in Dallas, Texas, is seeking a Pathologists' Assistant. In this position you will be responsible for accurate description of specimens, correct dissection for microscopic diagnosis and dictation of findings for pathologist's review. The ideal candidate will have a minimum of 3 years' experience. Prefer a degree from a NACCL-accredited Pathologist's Assistant program. AAPA Fellowship/ASCP also preferred. Applicants without a degree must be able to provide documentation showing eligibility under CLIA requirements for high complexity testing. The hours for this position are 6pm to 2:30am Monday - Friday. Benefits include Medical, Dental, Short and Long Term Disability Insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EOE For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 www.propath.com. Accessibility Accommodations If you require an accommodation to navigate our careers site, please send your request to accessibility@propath.com ______________________________________________ Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 norm.burnham@propath.com 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell To learn more about ProPath, please visit www.propath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From tony.henwood <@t> health.nsw.gov.au Wed Aug 1 19:16:58 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Aug 1 19:17:11 2012 Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D1CC2BF@xmdb02.nch.kids> Try a weak Harris's Haematoxylin stain (half the normal stain time in your H&E). The two blues are easy to distinguish. Harris's is preferred to Mayer's since Harris's seems to have a dark purple hue compared to the sky blue of Mayer's. You could even use a 1% neutral red in 1% acetic acid or even just a light eosin counterstain. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, 2 August 2012 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amosbrooks <@t> gmail.com Wed Aug 1 20:55:40 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Aug 1 20:55:44 2012 Subject: [Histonet] calcium buildup Message-ID: Hi, We have the same instrument. I love the thing to death! The water reservoirs can be removed by pulling them out like the other ones. The water input remains in place while the rubber gasket slides off. By the way, do this with the instrument off so it doesn't spill all over the place. Wash out the reservoir well then you can remove the water input by holding the large stainless steel nut at the back and twisting counter clockwise. If the calcium buildup is bad some channel lock pliers may come in handy. Don't loose the rubber gasket that is behind the stainless steel nut. The majority of the crud will come of with a scrub in detergent, but if it is really bad a soak in dilute HCl should help. You could leave it soaking overnight in hot water too. This would also be a good time to remove the stainless steel trays the reservoirs set on and clean out the drain. Fungal growths can build up there and can actually clog the drain if left unchecked. Actually this should all be part of a periodic maintenance plan. We have a service take care of it for us (Belair in New Jersey comes out for us) and I also take it down and clean it out before our Christmas break. Amos Brooks On Wed, Aug 1, 2012 at 11:25 AM, wrote: > > Message: 19 > Date: Wed, 1 Aug 2012 10:57:16 +0000 > From: "Molinari, Betsy" > Subject: [Histonet] calcium buildup > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > Hi, Does anyone know of a way to remove calcium buildup on the wash > nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. > Thank you. > > Betsy Molinari HT(ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) From amosbrooks <@t> gmail.com Wed Aug 1 21:09:06 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Aug 1 21:09:09 2012 Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Message-ID: Hi, We use Neutral Red in 1% solution. (Not being in the lab right now I think it is made up in 1% acetic acid, but I could be wrong.) It is much faster than NFR (nucear fast red). It is about one minute to stain as opposed to the 5 to 15 if the NFR solution is old. It is easy as heck to make up and neutral red has a much longer shelf life too. The only difference I have noted is that the stain is a slightly darker (rusty) red than the *really* light pink of NFR. Amos On Wed, Aug 1, 2012 at 1:00 PM, wrote: > Message: 4 > Date: Wed, 1 Aug 2012 12:50:14 -0400 > From: "Diana McCaig" > Subject: [Histonet] counterstain for Alcian Blue (ph2.5) > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Can you please let me know what options for counterstain there are other > than Nuclear Fast Red or a supplier who sells the prepared solution > > > > Diana > From nina.pries <@t> skane.se Thu Aug 2 06:37:55 2012 From: nina.pries <@t> skane.se (Pries Nina) Date: Thu Aug 2 06:38:04 2012 Subject: [Histonet] symphony another question Message-ID: We had Symphony on trial for about a year and found that it didn't suit our Lean organized lab. The machine is built to handle a large amount of trays at once (the tower and the different staining modules have great capacity) but for each tray you load the processing time increases with whatever time the baking is set to (we set it to 12 minutes because of problems with "flying" needle biopsies) since there is only ONE oven. We set the max number of trays to load at once to 4 but always ended up with a huge batch late in the day that got stained in the evening and had to be taken out and sorted in the morning >> uneven work load. Symphony is great in that it hardly requires any weekly maintanence at all and is ergonomically good, but on the other hand it continously wants to be fed boxes, large amounts of 99% ethanol and those stinky Clear bottles that are devilish (!) to open. Lots of waste packaging, and we at least had no way to separate waste for destruction (everything went into the sewage.) Huge footprint, nosiy, limonen smell (when changing bottles) and requires destilled water - it can't be placed just anywhere in the lab like the stainer we have now (Dako Coverstainer). Trays are loaded from two sides so they require constant turning by the histotech to be able to read slide labels, and after a while the springs holding the slides get broken off. Also, we had to specially adapt trays to hold supermega slides but the staining on those tended to be uneven. We also had continous problems with the mounting module - misaligned coverslips, bubbles, moisture. Little things that get annoying when handling several hundred slides each day! Too bad for a piece of hardware that promised such ease of use... Nina Pries Histotech Clinical Pathology Lund Sweden -----Ursprungligt meddelande----- Fr?n: Gudrun Lang [mailto:gu.lang@gmx.at] Skickat: den 1 augusti 2012 09:31 Till: histonet@lists.utsouthwestern.edu ?mne: [Histonet] symphony another question Hi! A question for Symphony-users. Do you enjoy working with the Symphony? Is it really a change to the better in comparison to other combined staining-coverslipping devices? I don't ask for quality or patient security - just for user concerns. Did it ever happen, that a run-error turned the system down (like it sometimes does with our Ultra)? What are the consequences? Just curious. Gudrun From vperez <@t> pathreflab.com Thu Aug 2 07:34:59 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Thu Aug 2 07:40:36 2012 Subject: [Histonet] negative controls In-Reply-To: References: Message-ID: Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nina.pries <@t> skane.se Thu Aug 2 08:54:52 2012 From: nina.pries <@t> skane.se (Pries Nina) Date: Thu Aug 2 08:55:09 2012 Subject: VB: [Histonet] symphony another question Message-ID: So far (we've only had it for a couple of months) the Dako Coverstainer is giving us much less trouble than Symphony did, there are no reagents that need to be replaced during the day (except for filling up coverslips), and we can work in the same room with it (very quiet and not smelly!). But there is indeed more maintanence involved, with bottles places very close to the floor. :( Nina Pries Histotech Clinical Pathology Lund Sweden ________________________________ Fr?n: Patricia Webster [mailto:PWEBSTER@swmail.sw.org] Skickat: den 2 augusti 2012 15:30 Till: Pries Nina ?mne: Re: [Histonet] symphony another question I think I misread the first time - so the Dako is ok? Trish Webster, HT(ASCP) Lead Anatomic Path Tech - Histology Scott & White Memorial Hospital 254-724-3695 MS-01-266 pwebster@swmail.sw.org >>> Pries Nina 8/2/2012 6:37 AM >>> We had Symphony on trial for about a year and found that it didn't suit our Lean organized lab. The machine is built to handle a large amount of trays at once (the tower and the different staining modules have great capacity) but for each tray you load the processing time increases with whatever time the baking is set to (we set it to 12 minutes because of problems with "flying" needle biopsies) since there is only ONE oven. We set the max number of trays to load at once to 4 but always ended up with a huge batch late in the day that got stained in the evening and had to be taken out and sorted in the morning >> uneven work load. Symphony is great in that it hardly requires any weekly maintanence at all and is ergonomically good, but on the other hand it continously wants to be fed boxes, large amounts of 99% ethanol and those stinky Clear bottles that are devilish (!) to open. Lots of waste packaging, and we at least had no way to separate waste for destruction (everything went into the sewage.) Huge footprint, nosiy, limonen smell (when changing bottles) and requires destilled water - it can't be placed just anywhere in the lab like the stainer we have now (Dako Coverstainer). Trays are loaded from two sides so they require constant turning by the histotech to be able to read slide labels, and after a while the springs holding the slides get broken off. Also, we had to specially adapt trays to hold supermega slides but the staining on those tended to be uneven. We also had continous problems with the mounting module - misaligned coverslips, bubbles, moisture. Little things that get annoying when handling several hundred slides each day! Too bad for a piece of hardware that promised such ease of use... Nina Pries Histotech Clinical Pathology Lund Sweden -----Ursprungligt meddelande----- Fr?n: Gudrun Lang [mailto:gu.lang@gmx.at] Skickat: den 1 augusti 2012 09:31 Till: histonet@lists.utsouthwestern.edu ?mne: [Histonet] symphony another question Hi! A question for Symphony-users. Do you enjoy working with the Symphony? Is it really a change to the better in comparison to other combined staining-coverslipping devices? I don't ask for quality or patient security - just for user concerns. Did it ever happen, that a run-error turned the system down (like it sometimes does with our Ultra)? What are the consequences? Just curious. Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> auburn.edu Thu Aug 2 09:51:23 2012 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Thu Aug 2 09:51:31 2012 Subject: [Histonet] RE: Hemo-De Message-ID: <7CED05D5DD63924196370F81D21F2E92097E9A94@exmb1.auburn.edu> Hello is anyone using Hemo=De as the clearing agent in the following stains: Bielschowsky's Method for Axis Cylinders & Dendrites and Sevier-Munger Method for Neural Tissues? Regards. Atoska From mjdessoye <@t> commonwealthhealth.net Thu Aug 2 09:58:15 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Thu Aug 2 09:58:31 2012 Subject: [Histonet] negative controls In-Reply-To: Message-ID: You may be able to omit them, but in our case they are still a detection that is part of our volume agreement. I'm not sure if everyone else has a similar agreement, but omitting the negative controls may affect your ability to meet a volume commitment. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Vanessa Perez [mailto:vperez@pathreflab.com] Sent: Thursday, August 02, 2012 8:35 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From katherine.o.murphy <@t> gmail.com Thu Aug 2 10:07:07 2012 From: katherine.o.murphy <@t> gmail.com (Katherine Murphy) Date: Thu Aug 2 10:07:15 2012 Subject: [Histonet] Rodent processing and artifactual spaces Message-ID: Hello Histo Gurus, I have a question for you: I process rat and mouse tissues (kidney, liver, lung, brain, pancreas, GI, spleen) and have been seeing a separation artifact in the tissues (esp. kidney, brain, and heart) on the slides. I believe this is what was described as artifactual spaces between individual cells or cell shrinkage, as described by Carson (1997) under Fixation and Processing. The processing schedule that has produced this is: 50% Reagent Alcohol (RA), 70% RA, 80% RA, 95% RA x 2, 100% RA x 2, xylene x 2, and Paraplast Plus @ 60?C with vacuum, at 40 min per station for rat and 25 min per station for mouse. Preceding processing is a 20? tap water rinse. Tissues have been in formalin for weeks in most cases but not always collected ideally (low formalin to tissue ratio, small jars used for large tissues, tissues submitted whole without slices or scores, etc)--we don't have much control over this part. Does anyone know what causes the separation artifact is and how it can be corrected? From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Aug 2 10:13:16 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Aug 2 10:14:02 2012 Subject: [Histonet] negative controls In-Reply-To: References: , Message-ID: I am hoping that the vendors will recognize this when the make these agreements. Hopefully not doubling our prices! Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@commonwealthhealth.net] Sent: Thursday, August 02, 2012 10:58 AM To: Vanessa Perez; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls You may be able to omit them, but in our case they are still a detection that is part of our volume agreement. I'm not sure if everyone else has a similar agreement, but omitting the negative controls may affect your ability to meet a volume commitment. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Vanessa Perez [mailto:vperez@pathreflab.com] Sent: Thursday, August 02, 2012 8:35 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> auburn.edu Thu Aug 2 11:11:45 2012 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Thu Aug 2 11:11:49 2012 Subject: [Histonet] RE: Hemo-De Message-ID: <7CED05D5DD63924196370F81D21F2E92097E9BE7@exmb1.auburn.edu> Hello, has anyone use or are you currently using Hemo-De as the clearing agent in these stains: 1) Bielschowsky's Method for Axis Cylinders and Dendrites and/or 2) Sevier-Munger Method for Neural Tissues? Your prompt reply will be much appreciated. Regards. ~ Atoska From rjbuesa <@t> yahoo.com Thu Aug 2 12:03:34 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 2 12:03:42 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: References: Message-ID: <1343927014.80295.YahooMailNeo@web121405.mail.ne1.yahoo.com> Rat and mice tissues do not have too much fat and are susceptible to dryness after processing specially when using xylene as "clearing" agent. I also think that your times are too long. The best processing protocol for rodent tissues is a sequence of 2-propanol ? propanol+mineral oil ? mineral oil ? paraffin. It has produced great results in those labs that are using it. If you are interested I can send you under separate cover the procedure. Ren? J. ________________________________ From: Katherine Murphy To: histonet@lists.utsouthwestern.edu Sent: Thursday, August 2, 2012 11:07 AM Subject: [Histonet] Rodent processing and artifactual spaces Hello Histo Gurus, I have a question for you: I process rat and mouse tissues (kidney, liver, lung, brain, pancreas, GI, spleen) and have been seeing a separation artifact in the tissues (esp. kidney, brain, and heart) on the slides. I believe this is what was described as artifactual spaces between individual cells or cell shrinkage, as described by Carson (1997) under Fixation and Processing. The processing schedule that has produced this is: 50% Reagent Alcohol (RA), 70% RA, 80% RA, 95% RA x 2, 100% RA x 2, xylene x 2, and Paraplast Plus @ 60?C with vacuum, at 40 min per station for rat and 25 min per station for mouse. Preceding processing is a 20? tap water rinse. Tissues have been in formalin for weeks in most cases but not always collected ideally (low formalin to tissue ratio, small jars used for large tissues, tissues submitted whole without slices or scores, etc)--we don't have much control over this part. Does anyone know what causes the separation artifact is and how it can be corrected? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu Aug 2 12:35:34 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Aug 2 12:35:47 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: <1343927014.80295.YahooMailNeo@web121405.mail.ne1.yahoo.com> References: <1343927014.80295.YahooMailNeo@web121405.mail.ne1.yahoo.com> Message-ID: <8CF3EEC5B259EA5-C0C-291E3@webmail-d083.sysops.aol.com> I think the processing times are fine, sorry Rene'. They are pretty similar to my processing schedules for both species. It could be more of a fixation artefact as you suspect. Tissues will begin to degrade waiting for formalin to get to them, and although formalin most tissues at about 3mm per hour - whole organs tend to slow penetration down somewhat. The low volume of fixative to tissue only compounds the problem. Jackie O' -----Original Message----- From: Rene J Buesa To: Katherine Murphy ; histonet Sent: Thu, Aug 2, 2012 12:03 pm Subject: Re: [Histonet] Rodent processing and artifactual spaces Rat and mice tissues do not have too much fat and are susceptible to dryness fter processing specially when using xylene as "clearing" agent. also think that your times are too long. he best processing protocol for rodent tissues is a sequence of 2-propanol ? ropanol+mineral oil ? mineral oil ? paraffin. t has produced great results in those labs that are using it. f you are interested I can send you under separate cover the procedure. en? J. _______________________________ rom: Katherine Murphy o: histonet@lists.utsouthwestern.edu ent: Thursday, August 2, 2012 11:07 AM ubject: [Histonet] Rodent processing and artifactual spaces Hello Histo Gurus, I have a question for you: I process rat and mouse tissues (kidney, liver, lung, brain, pancreas, I, spleen) and have been seeing a separation artifact in the tissues esp. kidney, brain, and heart) on the slides. I believe this is what as described as artifactual spaces between individual cells or cell hrinkage, as described by Carson (1997) under Fixation and rocessing. The processing schedule that has produced this is: 50% eagent Alcohol (RA), 70% RA, 80% RA, 95% RA x 2, 100% RA x 2, xylene 2, and Paraplast Plus @ 60?C with vacuum, at 40 min per station for at and 25 min per station for mouse. Preceding processing is a 20? ap water rinse. Tissues have been in formalin for weeks in most cases ut not always collected ideally (low formalin to tissue ratio, small ars used for large tissues, tissues submitted whole without slices or cores, etc)--we don't have much control over this part. Does anyone now what causes the separation artifact is and how it can be orrected? _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Aug 2 12:54:18 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Aug 2 12:54:24 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: <8CF3EEC5B259EA5-C0C-291E3@webmail-d083.sysops.aol.com> Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DAC6B@SBS2K8.premierlab.local> SSBhZ3JlZSB3aXRoIEphY2tpZSwgSSBwcm9jZXNzIGZyb20gMjAgdG8gMzAgbWludXRlcyBhIHN0 YXRpb24gZm9yIG1vdXNlIGFuZCByYXQgdGlzc3VlLCBidXQgSSBwcm9jZXNzIDQ1IG1pbnV0ZXMg cGVyIHN0YXRpb24gZm9yIGJyYWluIGFuZCBmb3Igc2tpbiBJIHByb2Nlc3Mgb25lIGhvdXIgcGVy 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b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVy bi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K From katherine.o.murphy <@t> gmail.com Thu Aug 2 13:39:48 2012 From: katherine.o.murphy <@t> gmail.com (Katherine Murphy) Date: Thu Aug 2 13:39:52 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: <14E2C6176416974295479C64A11CB9AE0162D07DAC6B@SBS2K8.premierlab.local> References: <8CF3EEC5B259EA5-C0C-291E3@webmail-d083.sysops.aol.com> <14E2C6176416974295479C64A11CB9AE0162D07DAC6B@SBS2K8.premierlab.local> Message-ID: Thanks for the suggestions. Just a note about the processing protocol...I just recently switched to the lower percentages of alcohol recently to try to remedy the horrible dryness and microchatter I was getting using a 70%, 95% X 2, 100% x 3, xylene x 3 set up where shortening processing times was not solving the problem! Changing the reagent set up solved the microchatter problem but now this other separation artifact has appeared. On 8/2/12, Elizabeth Chlipala wrote: > I agree with Jackie, I process from 20 to 30 minutes a station for mouse and > rat tissue, but I process 45 minutes per station for brain and for skin I > process one hour per station. I find that once you gross the tissue samples > in they need to be placed back into formalin to fix additionally and I never > gross mouse or rat tissue and place in 50 to 70% alcohol that just causes > problems, that?s been my experience. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > O'Connor > Sent: Thursday, August 02, 2012 11:36 AM > To: rjbuesa@yahoo.com; katherine.o.murphy@gmail.com; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Rodent processing and artifactual spaces > > > I think the processing times are fine, sorry Rene'. They are pretty > similar to my processing schedules for both species. It could be more of a > fixation artefact as you suspect. Tissues will begin to degrade waiting for > formalin to get to them, and although formalin most tissues at about 3mm per > hour - whole organs tend to slow penetration down somewhat. The low volume > of fixative to tissue only compounds the problem. > Jackie O' > > > > -----Original Message----- > From: Rene J Buesa > To: Katherine Murphy ; histonet > > Sent: Thu, Aug 2, 2012 12:03 pm > Subject: Re: [Histonet] Rodent processing and artifactual spaces > > > Rat and mice tissues do not have too much fat and are susceptible to > dryness > fter processing specially when using xylene as "clearing" agent. > also think that your times are too long. > he best processing protocol for rodent tissues is a sequence of 2-propanol > ? > ropanol+mineral oil ? mineral oil ? paraffin. > t has produced great results in those labs that are using it. > f you are interested I can send you under separate cover the procedure. > en? J. > > _______________________________ > rom: Katherine Murphy > o: histonet@lists.utsouthwestern.edu > ent: Thursday, August 2, 2012 11:07 AM > ubject: [Histonet] Rodent processing and artifactual spaces > Hello Histo Gurus, I have a question for you: > I process rat and mouse tissues (kidney, liver, lung, brain, pancreas, > I, spleen) and have been seeing a separation artifact in the tissues > esp. kidney, brain, and heart) on the slides. I believe this is what > as described as artifactual spaces between individual cells or cell > hrinkage, as described by Carson (1997) under Fixation and > rocessing. The processing schedule that has produced this is: 50% > eagent Alcohol (RA), 70% RA, 80% RA, 95% RA x 2, 100% RA x 2, xylene > 2, and Paraplast Plus @ 60?C with vacuum, at 40 min per station for > at and 25 min per station for mouse. Preceding processing is a 20? > ap water rinse. Tissues have been in formalin for weeks in most cases > ut not always collected ideally (low formalin to tissue ratio, small > ars used for large tissues, tissues submitted whole without slices or > cores, etc)--we don't have much control over this part. Does anyone > now what causes the separation artifact is and how it can be > orrected? > _______________________________________________ > istonet mailing list > istonet@lists.utsouthwestern.edu > ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > ______________________________________________ > istonet mailing list > istonet@lists.utsouthwestern.edu > ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b427297 <@t> aol.com Thu Aug 2 14:08:56 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Aug 2 14:09:16 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: References: <8CF3EEC5B259EA5-C0C-291E3@webmail-d083.sysops.aol.com> <14E2C6176416974295479C64A11CB9AE0162D07DAC6B@SBS2K8.premierlab.local> Message-ID: <8CF3EF965A703AA-11CC-29622@webmail-d059.sysops.aol.com> We trim our tisues the day after necropsy, put the cassettes back in formalin for a few hours on the processor before it starts. Our sections are durn perfect, if I do say so myself. One of the things I have found important for rodent tissues is to face the blocks and leave them on wet ice for about an hour before taking sections. No chatter whatsoever. We routinely cut at 5 microns. Jackie O' -----Original Message----- From: Katherine Murphy To: Elizabeth Chlipala Cc: Jackie O'Connor ; rjbuesa ; histonet Sent: Thu, Aug 2, 2012 1:39 pm Subject: Re: [Histonet] Rodent processing and artifactual spaces Thanks for the suggestions. Just a note about the processing rotocol...I just recently switched to the lower percentages of lcohol recently to try to remedy the horrible dryness and icrochatter I was getting using a 70%, 95% X 2, 100% x 3, xylene x 3 et up where shortening processing times was not solving the problem! hanging the reagent set up solved the microchatter problem but now his other separation artifact has appeared. On 8/2/12, Elizabeth Chlipala wrote: I agree with Jackie, I process from 20 to 30 minutes a station for mouse and rat tissue, but I process 45 minutes per station for brain and for skin I process one hour per station. I find that once you gross the tissue samples in they need to be placed back into formalin to fix additionally and I never gross mouse or rat tissue and place in 50 to 70% alcohol that just causes problems, that?s been my experience. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, August 02, 2012 11:36 AM To: rjbuesa@yahoo.com; katherine.o.murphy@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Rodent processing and artifactual spaces I think the processing times are fine, sorry Rene'. They are pretty similar to my processing schedules for both species. It could be more of a fixation artefact as you suspect. Tissues will begin to degrade waiting for formalin to get to them, and although formalin most tissues at about 3mm per hour - whole organs tend to slow penetration down somewhat. The low volume of fixative to tissue only compounds the problem. Jackie O' -----Original Message----- From: Rene J Buesa To: Katherine Murphy ; histonet Sent: Thu, Aug 2, 2012 12:03 pm Subject: Re: [Histonet] Rodent processing and artifactual spaces Rat and mice tissues do not have too much fat and are susceptible to dryness fter processing specially when using xylene as "clearing" agent. also think that your times are too long. he best processing protocol for rodent tissues is a sequence of 2-propanol ? ropanol+mineral oil ? mineral oil ? paraffin. t has produced great results in those labs that are using it. f you are interested I can send you under separate cover the procedure. en? J. _______________________________ rom: Katherine Murphy o: histonet@lists.utsouthwestern.edu ent: Thursday, August 2, 2012 11:07 AM ubject: [Histonet] Rodent processing and artifactual spaces Hello Histo Gurus, I have a question for you: I process rat and mouse tissues (kidney, liver, lung, brain, pancreas, I, spleen) and have been seeing a separation artifact in the tissues esp. kidney, brain, and heart) on the slides. I believe this is what as described as artifactual spaces between individual cells or cell hrinkage, as described by Carson (1997) under Fixation and rocessing. The processing schedule that has produced this is: 50% eagent Alcohol (RA), 70% RA, 80% RA, 95% RA x 2, 100% RA x 2, xylene 2, and Paraplast Plus @ 60?C with vacuum, at 40 min per station for at and 25 min per station for mouse. Preceding processing is a 20? ap water rinse. Tissues have been in formalin for weeks in most cases ut not always collected ideally (low formalin to tissue ratio, small ars used for large tissues, tissues submitted whole without slices or cores, etc)--we don't have much control over this part. Does anyone now what causes the separation artifact is and how it can be orrected? _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Aug 2 15:11:00 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Aug 2 15:11:03 2012 Subject: [Histonet] Used histology equipment Message-ID: Vendors! Please send me prices for a used: Leica microtome, a VIP 2000 tisssue processor (prefer floor standing) and an embedding center (older Shandon histocentre if I had my 'druthers). Cash, delivered to 78201. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From algranth <@t> email.arizona.edu Thu Aug 2 18:42:50 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Aug 2 18:42:55 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: <8CF3EF965A703AA-11CC-29622@webmail-d059.sysops.aol.com> References: <8CF3EEC5B259EA5-C0C-291E3@webmail-d083.sysops.aol.com> <14E2C6176416974295479C64A11CB9AE0162D07DAC6B@SBS2K8.premierlab.local> <8CF3EF965A703AA-11CC-29622@webmail-d059.sysops.aol.com> Message-ID: <31677299-2EE4-4FA4-8E8C-A23F9C85B5A6@email.arizona.edu> I process rodent tissue among other types of tissues and the times are similar to those that I use. In the place of Xylene I use Clear Rite 3. I find that this is a little more gentle and the tissue while still dry is not as bad and some things I don't even have to soak - like ovaries and some brains. But when I do soak I use a little glycerin in my water and let the faced blocks sit on the cold plate for 30 min-1 hr. Usually that is all it takes to get really pretty sections. One other thing to think of is the temp of your waterbath. I keep mine around 40 degrees C. I let the slides dry completely before putting them in the oven before staining. If you have water under the sections it could produce the some of the spaces that you are seeing when the water expands in the oven. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From Pat.Patterson <@t> propath.com Fri Aug 3 08:54:31 2012 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Fri Aug 3 08:54:35 2012 Subject: [Histonet] IHC Position Dallas Message-ID: <82C7248978CB50469FD6BA68EBBEFE67084B6103@exchange.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1-2 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 4:00 p.m. to 12:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From COPPINM <@t> aruplab.com Fri Aug 3 10:17:33 2012 From: COPPINM <@t> aruplab.com (Coppin, Margaret) Date: Fri Aug 3 10:17:40 2012 Subject: [Histonet] Tunel stain by IHC Message-ID: <448AA3F629599F4097B1E11A178B6C06A134E0@EXMBX1.aruplab.net> Hello everyone, I have a pathologist that is looking for a lab that performs the Tunel stain. I guess she wants to stain some research mouse samples to look for apoptosis. Thank you, Margaret G. Coppin, HT(ASCP) Technical Supervisor, Immunohistochemistry ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108 phone: (801)583-2787 X3869 email: coppinm@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From JWatson <@t> gnf.org Fri Aug 3 11:16:44 2012 From: JWatson <@t> gnf.org (James Watson) Date: Fri Aug 3 11:16:48 2012 Subject: [Histonet] Rodent processing and artifactual spaces In-Reply-To: References: <8CF3EEC5B259EA5-C0C-291E3@webmail-d083.sysops.aol.com> <14E2C6176416974295479C64A11CB9AE0162D07DAC6B@SBS2K8.premierlab.local> <8CF3EF965A703AA-11CC-29622@webmail-d059.sysops.aol.com> <31677299-2EE4-4FA4-8E8C-A23F9C85B5A6@email.arizona.edu> Message-ID: Something that I have done for years on animal tissues is: instead of 100% ETOH on the processor we use 5% glycerin in 100% ETOH. This keeps the tissue from becoming brittle and greatly decreases soaking time while cutting. Started doing this in the 80s at AFIP when processing 5 cm thick whole dog heart cross sections that took 48 hours to process. 2 minor problems with this: 1) does not work on fat, so we keep extra containers of 100% ETOH to switch to when processing fat. 2) we have to rinse our xylene recycling container and recycler monthly with 100% ETOH. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 02, 2012 4:43 PM Cc: HISTONET Subject: Re: [Histonet] Rodent processing and artifactual spaces I process rodent tissue among other types of tissues and the times are similar to those that I use. In the place of Xylene I use Clear Rite 3. I find that this is a little more gentle and the tissue while still dry is not as bad and some things I don't even have to soak - like ovaries and some brains. But when I do soak I use a little glycerin in my water and let the faced blocks sit on the cold plate for 30 min-1 hr. Usually that is all it takes to get really pretty sections. One other thing to think of is the temp of your waterbath. I keep mine around 40 degrees C. I let the slides dry completely before putting them in the oven before staining. If you have water under the sections it could produce the some of the spaces that you are seeing when the water expands in the oven. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> histocare.com Fri Aug 3 12:39:38 2012 From: contact <@t> histocare.com (Contact HistoCare) Date: Fri Aug 3 12:39:47 2012 Subject: [Histonet] Teabags Message-ID: <1C573345-D424-478D-9A45-0DDCF806DCD9@histocare.com> Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab From jamie.erickson <@t> abbott.com Fri Aug 3 14:30:18 2012 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Fri Aug 3 14:30:21 2012 Subject: [Histonet] Microchatter in mouse brain(isopropanol-storage solution) Message-ID: Hi All, I Hope someone can provide me with a little insight on a problem I'm having. I am do IHC on mouse brains that I fix for 8-12 hrs (trimmed after 2-3 hrs) in 10% NBF and processed overnight , embedded next day. My processor holds samples after fixation (8Hr) in 70% ETOH until it can be completed in the AM. My stations are 25min/ station + 1 Hr in paraffin (3X). If the study comes down on a Friday I hold samples in Isopropanol 70% at 4 degrees C for the weekend this was in the journal of histotechnology Journal of Histotechnology, Volume 34, Number 3, September 2011 , pp. 132-137(6). I'm noticing the brains are way to dry and I see a lot of microchatter on my sections. The IHC is good but these microchartter is difficult to deal with. I notice if I hold my block on wet ice for 1 hr they are not swollen, if this was a normal mouse brain w/o isopropanol it would be swollen up by then, so it is dry... So what is a guy to do? Do I reduce the Conc of isopropanol to say 40%.. I was running these samples right away. Fixed 6 hrs and processed right away and I didn't have this problem but coming back late at night to embed these studies was getting old.... So anyone have similar issues I'd like to hear how you resolved them. Extending fixation is possible but I'm not sure the effect on the IHC, I've already done 4 studies this way and I had to change but I need better sections Thanks, Jamie From b427297 <@t> aol.com Fri Aug 3 16:02:53 2012 From: b427297 <@t> aol.com (b427297@aol.com) Date: Fri Aug 3 16:03:18 2012 Subject: [Histonet] Microchatter in mouse brain(isopropanol-storage solution) In-Reply-To: References: Message-ID: <329BD633-E24A-4BF9-BE5D-90DF0955E101@aol.com> This is speculation on my part, but since your brain is about 60% lipid, you are depleting it by storage in alcohol. Most antibodies in my experience work well after 48 hours in formalin, so why don't you just let them stay in formalin over the weekend and diminish time in alcohol? Sent from my iPhone On Aug 3, 2012, at 2:30 PM, Jamie E Erickson wrote: > Hi All, > I Hope someone can provide me with a little insight on a > problem I'm having. > I am do IHC on mouse brains that I fix for 8-12 hrs (trimmed after 2-3 > hrs) in 10% NBF and processed overnight , embedded next day. > My processor holds samples after fixation (8Hr) in 70% ETOH until it can > be completed in the AM. My stations are 25min/ station + 1 Hr in paraffin > (3X). > > If the study comes down on a Friday I hold samples in Isopropanol 70% at > 4 degrees C for the weekend this was in the journal of histotechnology > Journal of Histotechnology, Volume 34, Number 3, September 2011 , pp. > 132-137(6). > I'm noticing the brains are way to dry and I see a lot of microchatter on > my sections. The IHC is good but these microchartter is difficult to deal > with. I notice if I hold my block on wet ice for 1 hr they are not > swollen, if this was a normal mouse brain w/o isopropanol it would be > swollen up by then, so it is dry... > > So what is a guy to do? Do I reduce the Conc of isopropanol to say 40%.. > I was running these samples right away. Fixed 6 hrs and processed right > away and I didn't have this problem but coming back late at night to > embed these studies was getting old.... > > So anyone have similar issues I'd like to hear how you resolved them. > Extending fixation is possible but I'm not sure the effect on the IHC, > I've already done 4 studies this way and I had to change but I need better > sections > > Thanks, > > Jamie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Sun Aug 5 18:04:50 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Aug 5 18:05:12 2012 Subject: [Histonet] Teabags In-Reply-To: <1C573345-D424-478D-9A45-0DDCF806DCD9@histocare.com> References: <1C573345-D424-478D-9A45-0DDCF806DCD9@histocare.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D1CCC50@xmdb02.nch.kids> Cheaper! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Saturday, 4 August 2012 3:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Teabags Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From wilson6848 <@t> yahoo.com Sun Aug 5 20:12:46 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Sun Aug 5 20:12:49 2012 Subject: [Histonet] Looking for a new position Message-ID: <1344215566.88274.YahooMailNeo@web125406.mail.ne1.yahoo.com> ??? I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY. Willing to relocate. ? ?Thanks, ? Wilson. From dahui_you <@t> yahoo.com Mon Aug 6 07:48:20 2012 From: dahui_you <@t> yahoo.com (Dahui You) Date: Mon Aug 6 07:48:24 2012 Subject: [Histonet] Re: Message-ID: <1344257300.31276.BPMail_high_noncarrier@web35703.mail.mud.yahoo.com> hi, do u see that, this site help me! http://nastrani.ic.cz/urworkcap.php?jfortuneid=10emo From campbellj <@t> muhlbauerlab.com Mon Aug 6 08:18:12 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Mon Aug 6 08:18:21 2012 Subject: [Histonet] Teabags In-Reply-To: <1C573345-D424-478D-9A45-0DDCF806DCD9@histocare.com> References: <1C573345-D424-478D-9A45-0DDCF806DCD9@histocare.com> Message-ID: We use actual teabags that we purchase in bulk. We filter the contents of our specimen bottles but instead of filtering into the teabag we make a cone-shape and filter onto the teabag and then neatly fold it to fit in a cassette. We are a derm lab so some of the shave biopsies we receive are curled. Once the pieces are cut at grossing we place them on a wet teabag and again neatly fold the teabag and place it in cassette. At embedding we open them on the warm area of the embedding center and don't have issues. The key for us is we put everything on the teabag not in it. Hope this helps! Jen Campbell On Fri, Aug 3, 2012 at 1:39 PM, Contact HistoCare wrote: > Hi all, > > Just a curiosity of mine, having contracted for many places I've seen many > different processes, some efficient and some inefficient. I find a lot of > labs do what they've always done just because they've always done something > a certain way for so long whether it's useful or not and generally are not > interested in change. > > One of these things I'm referring to is using teabags. I know some of you > LOVE them, but there are few things I loathe more than trying to dig out a > tiny biopsy sample from a teabag along with trying to open it while being > stuck together by the wax. > > Why in the world would anyone ever use teabags when there are > microcassettes and even biopsy cassettes? > > Please let me hear it. > > > www.HistoCare.com > Histology Staffing for your Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From contact <@t> histocare.com Mon Aug 6 08:32:16 2012 From: contact <@t> histocare.com (Contact HistoCare) Date: Mon Aug 6 08:32:32 2012 Subject: [Histonet] Teabags In-Reply-To: References: <1C573345-D424-478D-9A45-0DDCF806DCD9@histocare.com> Message-ID: I agree that putting tiny specimen *on* the teabag and not *in* it saves the embedded valuable time. For those that are budget conscious AND short on TAT, how well would *recycling* microcassettes work? Could they be successfully put back in the processing rack and cleaned during a normal rack cleaning? Wouldn't that be more cost-conscious than throwing away teabags since you can reuse those. Bear in mind, my questions are meant only to provoke a rethinking of some processes and consider not only convenience during grossing but also during embedding. On Aug 6, 2012, at 8:18 AM, Jennifer Campbell wrote: > We use actual teabags that we purchase in bulk. We filter the contents of our specimen bottles but instead of filtering into the teabag we make a cone-shape and filter onto the teabag and then neatly fold it to fit in a cassette. We are a derm lab so some of the shave biopsies we receive are curled. Once the pieces are cut at grossing we place them on a wet teabag and again neatly fold the teabag and place it in cassette. > > At embedding we open them on the warm area of the embedding center and don't have issues. > > The key for us is we put everything on the teabag not in it. > > Hope this helps! > > Jen Campbell > > On Fri, Aug 3, 2012 at 1:39 PM, Contact HistoCare wrote: > Hi all, > > Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. > > One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. > > Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? > > Please let me hear it. > > > www.HistoCare.com > Histology Staffing for your Lab > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Jen Campbell, HT(ASCP) > Supervisor of Technical Services > Muhlbauer Dermatopathology Laboratory > 61 Monroe Avenue, Ste B > Pittsford NY 14534 > P: 585.586.5166 > F: 585.586.3137 > > > IMPORTANT NOTICE: This e-mail and any attachments may contain confidential > or sensitive information which is, or may be, legally privileged or > otherwise protected by law from further disclosure. It is intended only > for the addressee. If you received this in error or from someone who was > not authorized to send it to you, please do not distribute, copy or use it > or any attachments. Please notify the sender immediately by reply e-mail > and delete this from your system. Thank you for your cooperation. > From Sandra.Harrison3 <@t> va.gov Mon Aug 6 08:51:52 2012 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Aug 6 08:52:25 2012 Subject: [Histonet] BOND IHC instrument- anyone experiencing multiple heater failures?? Message-ID: We have 2 Bond Max instruments and have experienced multiple heaters getting contaminated and corroded underneath, with a particulate matter, causing the heaters to fail. Our 2nd BOND Max is 3 years old and we have had to replace a total of 12 heaters (out of 30.) The only thing we do that is non-standard is we have been dewaxing off line, to save time. Does anyone else dewax off line? Is anyone else experiencing heater failures? We did a major P.M. 3 months ago, including changing the aspirating probe. That not-withstanding, we had to replace 5 more heaters last week. Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From TNMayer <@t> mdanderson.org Mon Aug 6 09:10:26 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Aug 6 09:11:11 2012 Subject: [Histonet] RE: Teabags (Contact HistoCare) Message-ID: You can use teabags to give specimens shape such as EMB and ECC. Those lose particles may not wash through a microcassette, but would just be loose pieces in there if not in a bag. You try to pick up those tiny pieces out of the corners. In a bag you can scrape them off, shape them and put them in the mold. Small individual samples such as GI biopsies can go in a biopsy cassette. Great question, I am going to add it to my exam for my students. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab From TGoins <@t> mt.gov Mon Aug 6 09:37:15 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Aug 6 09:37:25 2012 Subject: [Histonet] RE: Teabags In-Reply-To: References: Message-ID: I don't know if this would work for everyone, but we process our hair with skin scraping samples (animal source) in lens paper. We form a small packet with all folds on one side of the packet and embed the entire thing with the one layer of lens paper down. Keeps everything corralled so to speak. Tresa Goins Montana Veterinary Diagnostic Lab Bozeman, Montana 59718 406-994-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Monday, August 06, 2012 8:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Teabags (Contact HistoCare) You can use teabags to give specimens shape such as EMB and ECC. Those lose particles may not wash through a microcassette, but would just be loose pieces in there if not in a bag. You try to pick up those tiny pieces out of the corners. In a bag you can scrape them off, shape them and put them in the mold. Small individual samples such as GI biopsies can go in a biopsy cassette. Great question, I am going to add it to my exam for my students. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Mon Aug 6 09:48:21 2012 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Mon Aug 6 09:48:30 2012 Subject: [Histonet] BOND IHC instrument- anyone experiencing multiple heater failures?? In-Reply-To: References: Message-ID: <1344264501.35444.YahooMailNeo@web120706.mail.ne1.yahoo.com> Hi Sandra our Bond Max equipment is about that old as well and just recently we had one heater go out.? No other problems have occured, and yes we do dewax offline as well.?We are very rigid with our maintenance of the machine and have had good luck with the instrument overall. Michele Carr HTL ASCP Medical Laboratory Services ? ________________________________ From: "Harrison, Sandra C." To: histonet@lists.utsouthwestern.edu Sent: Monday, August 6, 2012 6:51 AM Subject: [Histonet] BOND IHC instrument- anyone experiencing multiple heater failures?? We have 2 Bond Max instruments and have experienced multiple heaters getting contaminated and corroded underneath, with a particulate matter, causing the heaters to fail.? Our 2nd BOND Max is 3 years old and we have had to replace a total of 12 heaters (out of 30.) The only thing we do that is non-standard is we have been dewaxing off line, to save time.? Does anyone else dewax off line?? Is anyone else experiencing heater failures? We did a major P.M. 3 months ago, including changing the aspirating probe.? That not-withstanding, we had to replace 5 more heaters last week.? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Aug 6 10:20:46 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Aug 6 10:20:59 2012 Subject: [Histonet] Control Blocks Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D1FA@BL2PRD0710MB363.namprd07.prod.outlook.com> Hello world... I am looking for a human colon cancer control BLOCK. I know I can get slides everywhere, but I need the block (that I can keep forever). Any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From careerstudio <@t> bellsouth.net Mon Aug 6 11:39:59 2012 From: careerstudio <@t> bellsouth.net (Career Studio) Date: Mon Aug 6 11:40:09 2012 Subject: [Histonet] leading laboratory in Tucson, AZ currently seeking a Grossing Technician Message-ID: <06be01cd73f2$1f1ee250$5d5ca6f0$@net> Our client is a leading laboratory in Tucson, AZ currently seeking a Grossing Technician to perform routine and non-routine activities involved in the preparation of surgical specimens for Histologic processing. Accountabilities for this role will be to: . Assure appropriate specimen accessioning and labeling; maintain the integrity of the specimen and patient identification. . Describe gross anatomic features of routine, uncomplicated surgical specimens, ensuring that all lesions, markings and sutures are mentioned. . Escalates non-routine, complicated surgical specimens to Pathologist Assistants. . Record measurements, weight, volume and any abnormal findings as applicable. . Dissect surgical specimens and prepare tissues for histologic processing. . Interact directly with pathologists regarding gross dissection/description activities, including defined specimen types that require direct supervision by a pathologist. . Identify problems that may adversely affect test performance or reporting of test results and either correct the problems or immediately notify the histology supervisor/manager, technical supervisor, or director. . Ensure corporate safety, quality control and quality assurance standards are met, as well as compliance with all local, federal, CLIA and CAP regulations. Individual l may be trained to perform basic Histology functions (i.e. embedding, microtomy, routine and special stains). Associates or Bachelors degree and 1 year histology and/or surgical gross experience required. This position offers competitive starting salary. Career Studio is one of the leading biotechnology search firms focused on the clinical laboratory sector. Please contact David King at biolabcareers@aol.com for more information. Career Studio national search 561-738-6363 Visit us on linkedin: http://www.linkedin.com/in/biotechnologyhires From TJohnson <@t> gnf.org Mon Aug 6 12:17:21 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Aug 6 12:17:27 2012 Subject: [Histonet] Dewaxing offline Message-ID: <9F3CFEE76E51B64991C7485270890B400CDCAB6B@EX4.lj.gnf.org> I have seen a couple emails where people who have the capability of dewaxing on the immunostainer but instead do the dewaxing offline. I am curious as to why you choose to do this. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From BDeBrosse-Serra <@t> isisph.com Mon Aug 6 12:19:09 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Aug 6 12:19:20 2012 Subject: [Histonet] RE: Dewaxing offline In-Reply-To: <9F3CFEE76E51B64991C7485270890B400CDCAB6B@EX4.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B400CDCAB6B@EX4.lj.gnf.org> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092BA8FCBC@EXCHMB01.isis.local> I was wondering about that myself......... Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Monday, August 06, 2012 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dewaxing offline I have seen a couple emails where people who have the capability of dewaxing on the immunostainer but instead do the dewaxing offline. I am curious as to why you choose to do this. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Aug 6 12:21:20 2012 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Aug 6 12:21:25 2012 Subject: [Histonet] RE: Control Blocks In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D1FA@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40E40@isexstore03> Sarah, Have you thought about using blocks that are about to be thrown out (after the required 10 year retention period)? We do this,because we know in-house controls are better than purchased due to having the same processing as our patient tissue to be tested. When we use these blocks, we mark the side with either the "tissue type" or with the stain that they will be used for. With having the original Case number still attached to the ct tissue, we can refer back to that case when asked my our Patholgist where the control tissue came from. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah DysartT Sent: Monday, August 06, 2012 11:21 AM To: histonet Subject: [Histonet] Control Blocks Hello world... I am looking for a human colon cancer control BLOCK. I know I can get slides everywhere, but I need the block (that I can keep forever). Any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * ======================== ======= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ======================== ======= From eca9 <@t> georgetown.edu Mon Aug 6 13:43:53 2012 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Mon Aug 6 13:44:17 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue Message-ID: Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University From eca9 <@t> georgetown.edu Mon Aug 6 13:48:25 2012 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Mon Aug 6 13:48:48 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D326@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D326@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: We are staining straight mouse tissue. On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: > Are you staining human xenografts in mice or straight out mouse tissue? I > get all mine from abcam. I am currently staining xenografts so I am using > a human specific one, but I think it might be good for mouse too? > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul > Sent: Monday, August 06, 2012 1:44 PM > To: histonet > Subject: [Histonet] Ki67 antibody to stain Mouse tissue > > Hello, > I am looking for a Ki67 to stain mouse tissues. We have been using Dako > M2749 but just found out it has been discontinued. Could anyone suggest a > good alternative? > Thank you all for any help, > Eva Permaul > Georgetown University > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jefthompson <@t> salud.unm.edu Mon Aug 6 13:59:24 2012 From: jefthompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Mon Aug 6 13:59:33 2012 Subject: [Histonet] opinion on heating slides prior to IHC Message-ID: <501FBFAC0200004D0012EAF2@hsc-iagate1.health.unm.edu> Dear Histonetters, We have an ongoing debate in our lab regarding preparing tissues for staining. I would like to get input from the wider community to use in our discussions. In all cases the tissues in question were from paraformaldehyde perfused rat brains that were sectioned at 10 or 20 microns on a cryostat and affixed to Superfrost Plus slides and stored in slide boxes within ziplock bags with dessicant at -80 C. So the debate is what happens when preparing to stain: One camp thinks that the slide box/bag should be warmed to 50 C before removing the slides to drive off any water from condensation on the tissues. Following this they will remove the slides to be stained and return the box to the freezer. After applying the PAP pen border then proceed with the staining protocol with serial alcohol rehydration as the first step. The other camp feels that heating the slides may potentially 'cook' the epitopes as well as being redundant since the first incubation in 100% EtOH will drive off any water which will be immediately added back in by the procedure anyway. They favor removing the frozen slides from the box and immediately returning the box with remaining slides to the freezer, and air drying the slides to be stained at RT before applying the PAP pen border The epitopes of interest are various intracellular and transmembrane cell type markers and enzymes common in rat neural tissue. Any input will be appreciated Thanks, J Thompson From shive003 <@t> umn.edu Mon Aug 6 14:59:21 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Aug 6 14:59:53 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D326@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: We have had success with Lab Vision's rabbit polyclonal on mouse tissue (catalog # RB-9043), utilizing HIER for antigen retrieval. Jan Shivers U of MN Vet Diag Lab On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul wrote: > We are staining straight mouse tissue. > > On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: > > > Are you staining human xenografts in mice or straight out mouse tissue? > I > > get all mine from abcam. I am currently staining xenografts so I am > using > > a human specific one, but I think it might be good for mouse too? > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul > > Sent: Monday, August 06, 2012 1:44 PM > > To: histonet > > Subject: [Histonet] Ki67 antibody to stain Mouse tissue > > > > Hello, > > I am looking for a Ki67 to stain mouse tissues. We have been using Dako > > M2749 but just found out it has been discontinued. Could anyone suggest a > > good alternative? > > Thank you all for any help, > > Eva Permaul > > Georgetown University > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From aeaton <@t> cellmarque.com Mon Aug 6 15:54:47 2012 From: aeaton <@t> cellmarque.com (Adrianna Eaton) Date: Mon Aug 6 15:54:53 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: Message-ID: Cell Marque's Ki-67 (SP6) works on mouse tissue. Please click on the link below for more information. http://www.cellmarque.com/cmc/P_antibodydetail.php?id=108&referrer=K Best Regards, Ms. Adrianna Eaton International Product Specialist 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8923 (707) 761-3270 (cell) (916) 746-8989 (fax) www.cellmarque.com From Passion to Product to Patient.? Learn with Cell Marque on:? ?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Monday, August 06, 2012 11:44 AM To: histonet Subject: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Aug 6 15:55:38 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 6 15:55:42 2012 Subject: [Histonet] opinion on heating slides prior to IHC In-Reply-To: <501FBFAC0200004D0012EAF2@hsc-iagate1.health.unm.edu> References: <501FBFAC0200004D0012EAF2@hsc-iagate1.health.unm.edu> Message-ID: <1344286538.74952.YahooMailNeo@web121406.mail.ne1.yahoo.com> I have done that many times and from my experience on the issue this us what I did and recommend you to do: 1- having slides at -80?C is OK but what is not OK is to warm them before taking the sldies out to re-freeze them again because you should never freeze-thaw-freeze any protein. 2- you take your box with the slides out of the freezer, remove the slides you are going to use and return the box with the rest of the slides to the freezer as quick as you can 3- thaw the slides to room temperature and after that heat them to help fixing the sections to the slides 4- that will not "cook" the epitopes that have already been fixed with the para formaldehyde. The epitopes have been already cross-linked and will not be denatured when heating the sections 5- the sections, as you describe, are FS from para formaldehyde perfused brain so they have NOT been dehydrated, so why do you need to "re-hydrate" what has not been dehydrated in the first place? From your description the "other camp" is closer to the best procedure. Ren? J. ________________________________ From: Jeffrey Thompson To: histonet@lists.utsouthwestern.edu Sent: Monday, August 6, 2012 2:59 PM Subject: [Histonet] opinion on heating slides prior to IHC Dear Histonetters, We have an ongoing debate in our lab regarding preparing tissues for staining. I would like to get input from the wider community to use in our discussions. In all cases the tissues in question were from paraformaldehyde perfused rat brains that were sectioned at 10 or 20 microns on a cryostat and affixed to Superfrost Plus slides and stored in slide boxes within ziplock bags with dessicant at -80 C. So the debate is what happens when preparing to stain: One camp thinks that the slide box/bag should be warmed to 50 C before removing the slides to drive off any water from condensation on the tissues. Following this they will remove the slides to be stained and return the box to the freezer. After applying the PAP pen border then proceed with the staining protocol with serial alcohol rehydration as the first step. The other camp feels that heating the slides may potentially 'cook' the epitopes as well as being redundant since the first incubation in 100% EtOH will drive off any water which will be immediately added back in by the procedure anyway. They favor removing the frozen slides from the box and immediately returning the box with remaining slides to the freezer, and air drying the slides to be stained at RT before applying the PAP pen border The epitopes of interest are various intracellular and transmembrane cell type markers and enzymes common in rat neural tissue. Any input will be appreciated Thanks, J Thompson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Mon Aug 6 15:57:53 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Aug 6 15:57:57 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D326@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <50202FD1.6000107@umn.edu> Thanks Jan, that would be the same one I have and it works great! Colleen Forster U of MN On 8/6/2012 2:59 PM, Jan Shivers wrote: > We have had success with Lab Vision's rabbit polyclonal on mouse tissue > (catalog # RB-9043), utilizing HIER for antigen retrieval. > > Jan Shivers > U of MN Vet Diag Lab > > On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul wrote: > >> We are staining straight mouse tissue. >> >> On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart wrote: >> >>> Are you staining human xenografts in mice or straight out mouse tissue? >> I >>> get all mine from abcam. I am currently staining xenografts so I am >> using >>> a human specific one, but I think it might be good for mouse too? >>> >>> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) >>> Histotechnologist >>> Mirna Therapeutics >>> 2150 Woodward Street >>> Suite 100 >>> Austin, Texas 78744 >>> (512)901-0900 ext. 6912 >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul >>> Sent: Monday, August 06, 2012 1:44 PM >>> To: histonet >>> Subject: [Histonet] Ki67 antibody to stain Mouse tissue >>> >>> Hello, >>> I am looking for a Ki67 to stain mouse tissues. We have been using Dako >>> M2749 but just found out it has been discontinued. Could anyone suggest a >>> good alternative? >>> Thank you all for any help, >>> Eva Permaul >>> Georgetown University >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cforster <@t> umn.edu Mon Aug 6 16:01:25 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Aug 6 16:01:28 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: <50202FD1.6000107@umn.edu> References: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D326@BL2PRD0710MB363.namprd07.prod.outlook.com> <50202FD1.6000107@umn.edu> Message-ID: <502030A5.50405@umn.edu> Just an FYI: Lab Vision antibodies HAVE to be ordered through Thermo Fisher. Lab Vision is a part of that company now. When you order antibodies from them you should use the Kalamazoo location as that is where the antibodies come from (I believe that is where Lab Vision was out of.) If you do this it will save you a LOT of headache. Colleen Forster U of MN On 8/6/2012 3:57 PM, Colleen Forster wrote: > Thanks Jan, that would be the same one I have and it works great! > > Colleen Forster > U of MN > > > On 8/6/2012 2:59 PM, Jan Shivers wrote: >> We have had success with Lab Vision's rabbit polyclonal on mouse tissue >> (catalog # RB-9043), utilizing HIER for antigen retrieval. >> >> Jan Shivers >> U of MN Vet Diag Lab >> >> On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul wrote: >> >>> We are staining straight mouse tissue. >>> >>> On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart >>> wrote: >>> >>>> Are you staining human xenografts in mice or straight out mouse >>>> tissue? >>> I >>>> get all mine from abcam. I am currently staining xenografts so I am >>> using >>>> a human specific one, but I think it might be good for mouse too? >>>> >>>> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) >>>> Histotechnologist >>>> Mirna Therapeutics >>>> 2150 Woodward Street >>>> Suite 100 >>>> Austin, Texas 78744 >>>> (512)901-0900 ext. 6912 >>>> >>>> >>>> -----Original Message----- >>>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul >>>> Sent: Monday, August 06, 2012 1:44 PM >>>> To: histonet >>>> Subject: [Histonet] Ki67 antibody to stain Mouse tissue >>>> >>>> Hello, >>>> I am looking for a Ki67 to stain mouse tissues. We have been using >>>> Dako >>>> M2749 but just found out it has been discontinued. Could anyone >>>> suggest a >>>> good alternative? >>>> Thank you all for any help, >>>> Eva Permaul >>>> Georgetown University >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> >>>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From rsrichmond <@t> gmail.com Mon Aug 6 16:42:32 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Aug 6 16:42:36 2012 Subject: [Histonet] Re: Teabags Message-ID: I've certainly used real tea bags - we got them from the Salada tea people - they're made of a paper made of abaca fiber, a very long-staple fiber. I still see many labs using that old standard, lens paper. About ten years ago I saw a lot of tissue bags, maybe made of nylon, available from whatever Shandon is called this week. I think embedders like them. You want the small size. A major disadvantage is that they're quite expensive. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) From ratliffjack <@t> hotmail.com Mon Aug 6 19:23:09 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Aug 6 19:23:15 2012 Subject: [Histonet] NATIONAL SOCIETY FOR HISTOTECHNOLOGY - HARD TISSUE FORUM EVENT AUGUST 18th in BETHESDA, MD!!!!!! Message-ID: The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You wont want to miss out on this opportunity to learn about and witness first hand all current forms of resin/plastics histology, as well as see for the first time ever here in North America a newly developed non-contact laser microtome! 7:30am - Registration Opens8:00am ? Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique ? Jack L Ratliff9:00am ? Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It ? Daniel S Perrien, Ph.D.10:30am ? Refreshment Break10:45am ? Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome ? Damien Laudier, BS, HTL(ASCP), QIHC12:15pm ? Lunch On Your Own1:15pm ? Ground Section Microtomy Techniques (Parts I & II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens ? (PART I) Joe Tabeling & Jack L Ratliff, BA; (PART II) Linda Durbin & Robert A Skinner2:45pm ? Refreshment Break3:00pm ? New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses ? Heiko Richter, Ph.D.4:00pm ? Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy ? Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack RatliffChairman, Hard Tissue Committee - National Society for Histotechnology From Susan.Walzer <@t> HCAHealthcare.com Tue Aug 7 02:15:38 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Aug 7 02:15:52 2012 Subject: [Histonet] RE: Teabags In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DF0CAC689@FWDCWPMSGCMS09.hca.corpad.net> I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like them because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Monday, August 06, 2012 10:37 AM To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Teabags I don't know if this would work for everyone, but we process our hair with skin scraping samples (animal source) in lens paper. We form a small packet with all folds on one side of the packet and embed the entire thing with the one layer of lens paper down. Keeps everything corralled so to speak. Tresa Goins Montana Veterinary Diagnostic Lab Bozeman, Montana 59718 406-994-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Monday, August 06, 2012 8:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Teabags (Contact HistoCare) You can use teabags to give specimens shape such as EMB and ECC. Those lose particles may not wash through a microcassette, but would just be loose pieces in there if not in a bag. You try to pick up those tiny pieces out of the corners. In a bag you can scrape them off, shape them and put them in the mold. Small individual samples such as GI biopsies can go in a biopsy cassette. Great question, I am going to add it to my exam for my students. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Aug 7 10:38:37 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Aug 7 10:38:42 2012 Subject: [Histonet] National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012! Message-ID: The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You wont want to miss out on this opportunity to learn about and witness first hand all current forms of resin/plastics histology, as well as see for the first time ever here in North America a newly developed non-contact laser microtome! 7:30am - Registration Opens 8:00am ? Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique ? Jack L Ratliff 9:00am ? Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It ? Daniel S Perrien, Ph.D. 10:30am ? Refreshment Break 10:45am ? Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome ? Damien Laudier, BS, HTL(ASCP), QIHC 12:15pm ? Lunch On Your Own 1:15pm ? Ground Section Microtomy Techniques (Parts I & II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens ? (PART I) Joe Tabeling & Jack L Ratliff, BA; (PART II) Linda Durbin & Robert A Skinner 2:45pm ? Refreshment Break 3:00pm ? New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses ? Heiko Richter, Ph.D. 4:00pm ? Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy ? Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack RatliffChairman, Hard Tissue Committee - National Society for Histotechnology From LStadler <@t> cbiolabs.com Tue Aug 7 10:40:44 2012 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Tue Aug 7 10:40:54 2012 Subject: [Histonet] Portable Clean Air System Message-ID: <98CC14B915EBA84B9A326D45CC3C1DECEC84B3C6@cbiolabs05.CBiolabs.local> All ~ Just curious if anyone has any experience with the "Airfiltronix" portable fume extractor filter? I saw it in the Ted Pella catalog, item # 3120, http://www.tedpella.com/safety_html/3120.htm It is designed to be used with a microwave, but can be used anywhere, according to the catalog. We are thinking about using something like this in our processing lab due to space constraints. Any input is greatly appreciated. Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 From TJohnson <@t> gnf.org Tue Aug 7 12:30:04 2012 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Aug 7 12:30:10 2012 Subject: [Histonet] Re: opinion on heating slides prior to IHC Message-ID: <9F3CFEE76E51B64991C7485270890B400CDCB1B7@EX4.lj.gnf.org> Dear J, I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process. I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it. As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to say they might have had to try multiple antibodies to find one that would work under those conditions? The only way to know if heat or other conditions will negatively affect your target protein is to test it empirically. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From jjohnson <@t> scripps.edu Tue Aug 7 14:11:53 2012 From: jjohnson <@t> scripps.edu (Jennifer Johnson) Date: Tue Aug 7 14:12:01 2012 Subject: [Histonet] Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? Message-ID: <9B9F464463CB36488E8904CF8336917FB76BB5708D@EXCH-CCR01.lj.ad.scripps.edu> Hi Guys Does anyone have a copy of the manual for the Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? Thanks in advance. Jennifer L. Johnson, Ph.D. Staff Scientist The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037 jjohnson@scripps.edu From rsrichmond <@t> gmail.com Tue Aug 7 14:22:57 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Aug 7 14:23:01 2012 Subject: [Histonet] Re: Teabags Message-ID: Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) From sdysart <@t> mirnarx.com Tue Aug 7 14:27:10 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Aug 7 14:27:20 2012 Subject: [Histonet] Re: Teabags In-Reply-To: References: Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D4D5@BL2PRD0710MB363.namprd07.prod.outlook.com> I use hair perm papers that I buy from a local beauty supply store. WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rclouse <@t> wellspan.org Tue Aug 7 14:31:36 2012 From: rclouse <@t> wellspan.org (Clouse, Rosanna) Date: Tue Aug 7 14:32:11 2012 Subject: [Histonet] Re: Teabags In-Reply-To: References: Message-ID: <9E4560B21D59954A9931FF35B7BCCDD4021A444967@EXCH01.wellspan.org> For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclouse@wellspan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From contact <@t> excaliburpathology.com Tue Aug 7 14:33:43 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Aug 7 14:33:47 2012 Subject: [Histonet] Re: Teabags In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D4D5@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D4D5@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <1344368023.96408.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Ditto! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Sarah Dysart To: Bob Richmond ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, August 7, 2012 2:27 PM Subject: RE: [Histonet] Re: Teabags I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Aug 7 15:04:53 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Aug 7 15:05:05 2012 Subject: [Histonet] Re: Teabags In-Reply-To: <1344368023.96408.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D4D5@BL2PRD0710MB363.namprd07.prod.outlook.com> <1344368023.96408.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE24B4555B779@MERCERMAIL.MercerU.local> Same here. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, August 07, 2012 3:34 PM To: Sarah Dysart; Histonet Subject: Re: [Histonet] Re: Teabags Ditto! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Sarah Dysart To: Bob Richmond ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, August 7, 2012 2:27 PM Subject: RE: [Histonet] Re: Teabags I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> histocare.com Tue Aug 7 17:10:25 2012 From: contact <@t> histocare.com (contact@histocare.com) Date: Tue Aug 7 17:10:30 2012 Subject: [Histonet] tissue highlighting for visibility Message-ID: <20120807181025.46opnxy2fok408sk@mail.histocare.com> Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ?What do some of you guys do? www.HistoCare.com Histology Staffing From toxpathnz <@t> gmail.com Tue Aug 7 20:56:08 2012 From: toxpathnz <@t> gmail.com (Alice Fraser) Date: Tue Aug 7 20:56:15 2012 Subject: [Histonet] decalcification of premolar teeth (dog) Message-ID: Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice From ratliffjack <@t> hotmail.com Tue Aug 7 22:12:35 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Aug 7 22:12:40 2012 Subject: [Histonet] National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! Message-ID: Greetings! The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You won't want to miss out on this opportunity to learn about and witness via "LIVE" demonstration all current forms of resin/plastics histology equipment. A special treat this year will be a first ever showing and demonstration in North America of a newly developed non-contact laser microtome (TissueSurgeon) to cut micron thin sections of a variety of tissue types! 7:30am - Registration Opens 8:00am ? Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique ? Jack L Ratliff 9:00am ? Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It ? Daniel S Perrien, Ph.D. 10:30am ? Refreshment Break 10:45am ? Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome ? Damien Laudier, BS, HTL(ASCP), QIHC 12:15pm ? Lunch On Your Own 1:15pm ? Ground Section Microtomy Techniques (Parts I & II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens ? (PART I) Joe Tabeling & Jack L Ratliff, BA; (PART II) Linda Durbin & Robert A Skinner 2:45pm ? Refreshment Break 3:00pm ? New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses ? Heiko Richter, Ph.D. 4:00pm ? Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy ? Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology From meghak <@t> g.clemson.edu Tue Aug 7 22:45:47 2012 From: meghak <@t> g.clemson.edu (Megha Kumar) Date: Tue Aug 7 22:45:50 2012 Subject: [Histonet] help ! paraffin section Message-ID: Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * From lpwenk <@t> sbcglobal.net Wed Aug 8 04:33:45 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Aug 8 04:33:55 2012 Subject: [Histonet] tissue highlighting for visibility In-Reply-To: <20120807181025.46opnxy2fok408sk@mail.histocare.com> References: <20120807181025.46opnxy2fok408sk@mail.histocare.com> Message-ID: <75FEA5CFE2B246ED8F5B9D00FD2282A9@HP2010> Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Aug 8 04:36:19 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Aug 8 04:36:28 2012 Subject: [Histonet] Re: Teabags In-Reply-To: <9E4560B21D59954A9931FF35B7BCCDD4021A444967@EXCH01.wellspan.org> References: <9E4560B21D59954A9931FF35B7BCCDD4021A444967@EXCH01.wellspan.org> Message-ID: Hint when using these - do NOT try to fold them up into a nice looking square. Once processed and in paraffin, it is very difficult to find the edge, to try to open back up. Fold into a not nice to look at, off-set square that is slightly crumpled. Much easier to find the edge. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on Beaumont Hospital. -----Original Message----- From: Clouse, Rosanna Sent: Tuesday, August 07, 2012 3:31 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Teabags For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclouse@wellspan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmendell <@t> goldbergmd.net Wed Aug 8 05:47:56 2012 From: kmendell <@t> goldbergmd.net (MaryK Mendell) Date: Wed Aug 8 05:48:58 2012 Subject: [Histonet] tissue highlighting for visibility In-Reply-To: <75FEA5CFE2B246ED8F5B9D00FD2282A9@HP2010> References: <20120807181025.46opnxy2fok408sk@mail.histocare.com>, <75FEA5CFE2B246ED8F5B9D00FD2282A9@HP2010> Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D328@EXMBX01.mmeprod.cbeyond> ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: contact@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Wed Aug 8 07:11:13 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed Aug 8 07:11:19 2012 Subject: [Histonet] Tissue Processor Message-ID: I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From vperez <@t> pathreflab.com Wed Aug 8 07:53:24 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Wed Aug 8 07:59:29 2012 Subject: [Histonet] tissue highlighting for visibility In-Reply-To: <96138C8AB728814B9A576E4364EF84F9012BD954D328@EXMBX01.mmeprod.cbeyond> References: <20120807181025.46opnxy2fok408sk@mail.histocare.com>, <75FEA5CFE2B246ED8F5B9D00FD2282A9@HP2010> <96138C8AB728814B9A576E4364EF84F9012BD954D328@EXMBX01.mmeprod.cbeyond> Message-ID: We use microwave processing and we add hematoxylin to the absolute and eosin to the isopropyl....this also helps in keeping techs from accidently using isopropyl as absolute or vice-versa... when grosser cant find tissue in the container we put a drop of eosin and swirl and filter to try and find any material in the formalin container. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryK Mendell Sent: Wednesday, August 08, 2012 5:48 AM To: Lee & Peggy Wenk; contact@histocare.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissue highlighting for visibility ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: contact@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 8 09:25:22 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 8 09:25:29 2012 Subject: [Histonet] Tissue Processor In-Reply-To: References: Message-ID: <1344435922.89103.YahooMailNeo@web121405.mail.ne1.yahoo.com> Sakura Ren? J. ________________________________ From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, August 8, 2012 8:11 AM Subject: [Histonet] Tissue Processor I am going to need to purchase a new tissue processor mine keeps breaking down.? What tissue processor would you buy and why?? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 8 09:32:39 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 8 09:32:47 2012 Subject: [Histonet] tissue highlighting for visibility In-Reply-To: <20120807181025.46opnxy2fok408sk@mail.histocare.com> References: <20120807181025.46opnxy2fok408sk@mail.histocare.com> Message-ID: <1344436359.627.YahooMailNeo@web121402.mail.ne1.yahoo.com> I always used few drops of alcoholic eosin in the 70%EthOL, just enough to make the solution a "pale pink". That amount is enough to give a faint "pink hue" to the tissue to ease its localization. This does not interfere with any stain done after wards. Ren? J. ________________________________ From: "contact@histocare.com" To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 6:10 PM Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ?What do some of you guys do? http://www.histocare.com/ Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Aug 8 09:29:29 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Aug 8 09:32:55 2012 Subject: [Histonet] decalcification of premolar teeth (dog) In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388D4F@UTHCMS1.uthouston.edu> Alice I would recommend using sodium formate/formic acid mixture for demineralization as this is more gentle than most agents. I would not use hydrochloric acid unless you are shipwrecked on a desert island and that is the only chemical available to you. I am assuming that EDTA demineralization is not an option for you? Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alice Fraser [toxpathnz@gmail.com] Sent: Tuesday, August 07, 2012 8:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of premolar teeth (dog) Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 8 09:48:32 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 8 09:48:36 2012 Subject: [Histonet] help ! paraffin section In-Reply-To: References: Message-ID: <1344437312.51180.YahooMailNeo@web121405.mail.ne1.yahoo.com> What you describe is a typical example of poor paraffin infiltration = the paraffin has not infiltrated the tissue and when you prepare the final block it will consist of 2 different components; the tissue and the paraffin. That is why you end with a "good paraffin section" without the tissue. Poor paraffin infiltration is always caused by an improper sequence while tissue processing. Either the fixation is incomplete OR the dehydration is incomplete and there is water in the tissue when you go to the "clearing" stage OR the clearing stage is incomplete and the tissue still has alcohol (immiscible with paraffin) when the tissue goes to the paraffin OR the paraffin infiltration is too short. The problem resides in your processing protocol and there is nothing you can do about that?at the end. Try to check your processing protocol to eliminate the problem. If this is happening "all of the sudden" while you used to have good results previously, then you either have changed reagents or the reagents?are not in a good condition. Ren? J.? ________________________________ From: Megha Kumar To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 11:45 PM Subject: [Histonet] help ! paraffin section Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Wed Aug 8 10:08:30 2012 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Aug 8 10:08:39 2012 Subject: [Histonet] help ! paraffin section In-Reply-To: References: Message-ID: In addition to Rene's comment, to cut coagulated tissue (skin that have new wound crust) and calcified tissue is difficult. On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar wrote: > Hi All > I am trying to section adult mouse intestine and skin using paraffin > embedding. However, when i section, the tissue is torn although the rest of > the paraffin looks perfect. Please suggest why this is happening. Also, > sometimes the skin sections fall off the slides when I perform in situ > hybridization. Any ideas how to prevent this? > Please help! i am a beginner in histology and dont' know what to do! > regards > Megha > > > * > * > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 From eca9 <@t> georgetown.edu Wed Aug 8 10:38:48 2012 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Wed Aug 8 10:39:15 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: Message-ID: Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been verified on Human tissues. Secondly had anyone used it on mouse gut. Does it stain in the bottom of the crypts or at the top? I am asking because I have also tried a different antibody from Leica but got staining at the top of the crypts not at the bottom. Thank you to everyone for all of your valuable suggestions and advice, Eva Permaul Georgetown University On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < joost.bruijntjes@tno.triskelion.nl> wrote: > Hi Eva > > I've used an antibody from Labvision /Neomarkers. It is a rabbit > monoclonal; catalog number: RM-9106. > > Joost > > -----Oorspronkelijk bericht----- > Van: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] Namens Eva Permaul > Verzonden: maandag 6 augustus 2012 20:44 > Aan: histonet > Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue > > Hello, > I am looking for a Ki67 to stain mouse tissues. We have been using Dako > M2749 but just found out it has been discontinued. Could anyone suggest a > good alternative? > Thank you all for any help, > Eva Permaul > Georgetown University > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dorothy.L.Webb <@t> HealthPartners.Com Wed Aug 8 10:42:42 2012 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Aug 8 10:42:49 2012 Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 9 In-Reply-To: <46a71400-6abc-4d05-aa3f-83b31c63a819@hpech2.HealthPartners.int> References: <46a71400-6abc-4d05-aa3f-83b31c63a819@hpech2.HealthPartners.int> Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4301B5BAD1497C@HPEMX3.HealthPartners.int> We make up a "marking Eosin" that we use on small bx before we place them in cassettes. We do not use actual teabags, but the mesh bags from Thermo Fisher that are not hard to pull apart and we never have anything sticking I the corners. I do know what you are referring to as some of the mesh bags ot teabags on the market are not mad of as fine material and are hard to pull apart and allow for specimens to gather in the corners. Our mesh bags are used for all specimens the PA's can "pour" into the bags. We also use the Obex papers for needle bx type specimens that can be laid out nicely on the paper and folded over the tissue once for optimal processing. On tissue processors, we are new this year to the Peloris processor from Leica and love the versatility of two retorts and the great reagent management which allows for less waste and less maintenance tech time. I was always a Sakura VIP fan previous, still am, but cannot compare the two retort option and how LEAN it has mad our lab! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 08, 2012 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: opinion on heating slides prior to IHC (Teri Johnson) 2. Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? (Jennifer Johnson) 3. Re: Teabags (Bob Richmond) 4. RE: Re: Teabags (Sarah Dysart) 5. RE: Re: Teabags (Clouse, Rosanna) 6. Re: Re: Teabags (Paula Pierce) 7. RE: Re: Teabags (Shirley A. Powell) 8. tissue highlighting for visibility (contact@histocare.com) 9. decalcification of premolar teeth (dog) (Alice Fraser) 10. National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! (Jack Ratliff) 11. help ! paraffin section (Megha Kumar) 12. Re: tissue highlighting for visibility (Lee & Peggy Wenk) 13. Re: Re: Teabags (Lee & Peggy Wenk) 14. RE: tissue highlighting for visibility (MaryK Mendell) 15. Tissue Processor (Heckford, Karen - SMMC-SF) 16. RE: tissue highlighting for visibility (Vanessa Perez) 17. Re: Tissue Processor (Rene J Buesa) 18. Re: tissue highlighting for visibility (Rene J Buesa) 19. RE: decalcification of premolar teeth (dog) (Rittman, Barry R) 20. Re: help ! paraffin section (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Aug 2012 17:30:04 +0000 From: Teri Johnson Subject: [Histonet] Re: opinion on heating slides prior to IHC To: "jefthompson@salud.unm.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B400CDCB1B7@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear J, I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process. I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it. As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to say they might have had to try multiple antibodies to find one that would work under those conditions? The only way to know if heat or other conditions will negatively affect your target protein is to test it empirically. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 2 Date: Tue, 7 Aug 2012 12:11:53 -0700 From: Jennifer Johnson Subject: [Histonet] Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? To: "histonet@lists.utsouthwestern.edu" Message-ID: <9B9F464463CB36488E8904CF8336917FB76BB5708D@EXCH-CCR01.lj.ad.scripps.edu> Content-Type: text/plain; charset="us-ascii" Hi Guys Does anyone have a copy of the manual for the Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? Thanks in advance. Jennifer L. Johnson, Ph.D. Staff Scientist The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037 jjohnson@scripps.edu ------------------------------ Message: 3 Date: Tue, 7 Aug 2012 15:22:57 -0400 From: Bob Richmond Subject: [Histonet] Re: Teabags To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ------------------------------ Message: 4 Date: Tue, 7 Aug 2012 19:27:10 +0000 From: Sarah Dysart Subject: RE: [Histonet] Re: Teabags To: Bob Richmond , "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5018D4D4D5@BL2PRD0710MB363.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" I use hair perm papers that I buy from a local beauty supply store. WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 7 Aug 2012 15:31:36 -0400 From: "Clouse, Rosanna" Subject: RE: [Histonet] Re: Teabags To: "'Histonet@lists.utsouthwestern.edu'" Message-ID: <9E4560B21D59954A9931FF35B7BCCDD4021A444967@EXCH01.wellspan.org> Content-Type: text/plain; charset="us-ascii" For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclouse@wellspan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ ------------------------------ Message: 6 Date: Tue, 7 Aug 2012 12:33:43 -0700 (PDT) From: Paula Pierce Subject: Re: [Histonet] Re: Teabags To: Sarah Dysart , Histonet Message-ID: <1344368023.96408.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Ditto! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Sarah Dysart To: Bob Richmond ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, August 7, 2012 2:27 PM Subject: RE: [Histonet] Re: Teabags I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 7 Aug 2012 16:04:53 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] Re: Teabags To: Paula Pierce , Sarah Dysart , Histonet Message-ID: <9BF995BC0E47744E9673A41486E24EE24B4555B779@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" Same here. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, August 07, 2012 3:34 PM To: Sarah Dysart; Histonet Subject: Re: [Histonet] Re: Teabags Ditto! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Sarah Dysart To: Bob Richmond ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, August 7, 2012 2:27 PM Subject: RE: [Histonet] Re: Teabags I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 07 Aug 2012 18:10:25 -0400 From: contact@histocare.com Subject: [Histonet] tissue highlighting for visibility To: Histonet@lists.utsouthwestern.edu Message-ID: <20120807181025.46opnxy2fok408sk@mail.histocare.com> Content-Type: text/plain; charset=UTF-8 Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ??What do some of you guys do? www.HistoCare.com Histology Staffing ------------------------------ Message: 9 Date: Wed, 8 Aug 2012 13:56:08 +1200 From: Alice Fraser Subject: [Histonet] decalcification of premolar teeth (dog) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice ------------------------------ Message: 10 Date: Tue, 7 Aug 2012 23:12:35 -0400 From: Jack Ratliff Subject: [Histonet] National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! To: Histonet Message-ID: Content-Type: text/plain; charset="Windows-1252" Greetings! The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You won't want to miss out on this opportunity to learn about and witness via "LIVE" demonstration all current forms of resin/plastics histology equipment. A special treat this year will be a first ever showing and demonstration in North America of a newly developed non-contact laser microtome (TissueSurgeon) to cut micron thin sections of a variety of tissue types! 7:30am - Registration Opens 8:00am ? Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique ? Jack L Ratliff 9:00am ? Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It ? Daniel S Perrien, Ph.D. 10:30am ? Refreshment Break 10:45am ? Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome ? Damien Laudier, BS, HTL(ASCP), QIHC 12:15pm ? Lunch On Your Own 1:15pm ? Ground Section Microtomy Techniques (Parts I & II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens ? (PART I) Joe Tabeling & Jack L Ratliff, BA; (PART II) Linda Durbin & Robert A Skinner 2:45pm ? Refreshment Break 3:00pm ? New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses ? Heiko Richter, Ph.D. 4:00pm ? Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy ? Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology ------------------------------ Message: 11 Date: Wed, 8 Aug 2012 09:15:47 +0530 From: Megha Kumar Subject: [Histonet] help ! paraffin section To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * ------------------------------ Message: 12 Date: Wed, 8 Aug 2012 05:33:45 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] tissue highlighting for visibility To: , Message-ID: <75FEA5CFE2B246ED8F5B9D00FD2282A9@HP2010> Content-Type: text/plain; format=flowed; charset="utf-8"; reply-type=original Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 8 Aug 2012 05:36:19 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Re: Teabags To: "Clouse, Rosanna" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hint when using these - do NOT try to fold them up into a nice looking square. Once processed and in paraffin, it is very difficult to find the edge, to try to open back up. Fold into a not nice to look at, off-set square that is slightly crumpled. Much easier to find the edge. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on Beaumont Hospital. -----Original Message----- From: Clouse, Rosanna Sent: Tuesday, August 07, 2012 3:31 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Teabags For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclouse@wellspan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 8 Aug 2012 06:47:56 -0400 From: MaryK Mendell Subject: RE: [Histonet] tissue highlighting for visibility To: Lee & Peggy Wenk , "contact@histocare.com" , "Histonet@lists.utsouthwestern.edu" Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D328@EXMBX01.mmeprod.cbeyond> Content-Type: text/plain; charset="us-ascii" ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: contact@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 8 Aug 2012 05:11:13 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Tissue Processor To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 16 Date: Wed, 8 Aug 2012 07:53:24 -0500 From: Vanessa Perez Subject: RE: [Histonet] tissue highlighting for visibility To: MaryK Mendell , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use microwave processing and we add hematoxylin to the absolute and eosin to the isopropyl....this also helps in keeping techs from accidently using isopropyl as absolute or vice-versa... when grosser cant find tissue in the container we put a drop of eosin and swirl and filter to try and find any material in the formalin container. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryK Mendell Sent: Wednesday, August 08, 2012 5:48 AM To: Lee & Peggy Wenk; contact@histocare.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissue highlighting for visibility ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: contact@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 8 Aug 2012 07:25:22 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Tissue Processor To: "Heckford, Karen - SMMC-SF" , "histonet@lists.utsouthwestern.edu" Message-ID: <1344435922.89103.YahooMailNeo@web121405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Sakura Ren? J. ________________________________ From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, August 8, 2012 8:11 AM Subject: [Histonet] Tissue Processor I am going to need to purchase a new tissue processor mine keeps breaking down.? What tissue processor would you buy and why?? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 8 Aug 2012 07:32:39 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] tissue highlighting for visibility To: "contact@histocare.com" , "Histonet@lists.utsouthwestern.edu" Message-ID: <1344436359.627.YahooMailNeo@web121402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I always used few drops of alcoholic eosin in the 70%EthOL, just enough to make the solution a "pale pink". That amount is enough to give a faint "pink hue" to the tissue to ease its localization. This does not interfere with any stain done after wards. Ren? J. ________________________________ From: "contact@histocare.com" To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 6:10 PM Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ?What do some of you guys do? http://www.histocare.com/ Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 8 Aug 2012 09:29:29 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] decalcification of premolar teeth (dog) To: "histonet@lists.utsouthwestern.edu" Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388D4F@UTHCMS1.uthouston.edu> Content-Type: text/plain; charset="us-ascii" Alice I would recommend using sodium formate/formic acid mixture for demineralization as this is more gentle than most agents. I would not use hydrochloric acid unless you are shipwrecked on a desert island and that is the only chemical available to you. I am assuming that EDTA demineralization is not an option for you? Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alice Fraser [toxpathnz@gmail.com] Sent: Tuesday, August 07, 2012 8:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of premolar teeth (dog) Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 8 Aug 2012 07:48:32 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] help ! paraffin section To: Megha Kumar , "histonet@lists.utsouthwestern.edu" Message-ID: <1344437312.51180.YahooMailNeo@web121405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 What you describe is a typical example of poor paraffin infiltration = the paraffin has not infiltrated the tissue and when you prepare the final block it will consist of 2 different components; the tissue and the paraffin. That is why you end with a "good paraffin section" without the tissue. Poor paraffin infiltration is always caused by an improper sequence while tissue processing. Either the fixation is incomplete OR the dehydration is incomplete and there is water in the tissue when you go to the "clearing" stage OR the clearing stage is incomplete and the tissue still has alcohol (immiscible with paraffin) when the tissue goes to the paraffin OR the paraffin infiltration is too short. The problem resides in your processing protocol and there is nothing you can do about that?at the end. Try to check your processing protocol to eliminate the problem. If this is happening "all of the sudden" while you used to have good results previously, then you either have changed reagents or the reagents?are not in a good condition. Ren? J.? ________________________________ From: Megha Kumar To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 11:45 PM Subject: [Histonet] help ! paraffin section Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 9 **************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From eca9 <@t> georgetown.edu Wed Aug 8 10:51:59 2012 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Wed Aug 8 10:52:26 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: Message-ID: I just found a nice publication with the Rm-9106 on mouse intestine. Have provided it to my manager. Thank you everyone. Eva On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul wrote: > Just to clarify. Are you using it on mouse tissues? I am only asking > because the Thermo Scientific/Labvision/Neomarkers data sheet says it has > only been verified on Human tissues. > Secondly had anyone used it on mouse gut. Does it stain in the bottom of > the crypts or at the top? I am asking because I have also tried a different > antibody from Leica but got staining at the top of the crypts not at the > bottom. > > Thank you to everyone for all of your valuable suggestions and advice, > Eva Permaul > Georgetown University > > > On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < > joost.bruijntjes@tno.triskelion.nl> wrote: > >> Hi Eva >> >> I've used an antibody from Labvision /Neomarkers. It is a rabbit >> monoclonal; catalog number: RM-9106. >> >> Joost >> >> -----Oorspronkelijk bericht----- >> Van: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] Namens Eva Permaul >> Verzonden: maandag 6 augustus 2012 20:44 >> Aan: histonet >> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue >> >> Hello, >> I am looking for a Ki67 to stain mouse tissues. We have been using Dako >> M2749 but just found out it has been discontinued. Could anyone suggest a >> good alternative? >> Thank you all for any help, >> Eva Permaul >> Georgetown University >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From cforster <@t> umn.edu Wed Aug 8 11:25:46 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Wed Aug 8 11:25:46 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: Message-ID: <5022930A.3060203@umn.edu> FYI: That is what the data sheet says because they didn't test it but many of us have and it works beautifully! It should stain mostly in the bottom of the crypts where the new cells emerge from but you will have occasional cells towards the top of the crypt. It should look much like it does in human gut and yes, I have done it on most major organs of the mouse. Colleen Forster U of MN On 8/8/2012 10:38 AM, Eva Permaul wrote: > Just to clarify. Are you using it on mouse tissues? I am only asking > because the Thermo Scientific/Labvision/Neomarkers data sheet says it has > only been verified on Human tissues. > Secondly had anyone used it on mouse gut. Does it stain in the bottom of > the crypts or at the top? I am asking because I have also tried a different > antibody from Leica but got staining at the top of the crypts not at the > bottom. > > Thank you to everyone for all of your valuable suggestions and advice, > Eva Permaul > Georgetown University > > On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < > joost.bruijntjes@tno.triskelion.nl> wrote: > >> Hi Eva >> >> I've used an antibody from Labvision /Neomarkers. It is a rabbit >> monoclonal; catalog number: RM-9106. >> >> Joost >> >> -----Oorspronkelijk bericht----- >> Van: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] Namens Eva Permaul >> Verzonden: maandag 6 augustus 2012 20:44 >> Aan: histonet >> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue >> >> Hello, >> I am looking for a Ki67 to stain mouse tissues. We have been using Dako >> M2749 but just found out it has been discontinued. Could anyone suggest a >> good alternative? >> Thank you all for any help, >> Eva Permaul >> Georgetown University >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mucram11 <@t> comcast.net Wed Aug 8 11:28:13 2012 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Aug 8 11:28:18 2012 Subject: [Histonet] Tissue Processor In-Reply-To: Message-ID: <1975763389.154932.1344443293014.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We have swithched from the VIP Sakura, older models, to the Thermo Excelsior.? We are currently buying our 3rd and 4th units for the Histology Lab.? They have features we really liked and cut my our exposure to formalin and xylene by almost 90% due to the way the system changes solutions.? Due to the very gently agitation used during processing we were also able to cut some time off our programs for overnight and short cycle biopsy runs.? We stil use a Leica ASP 300 for bone marrows only as it is what need due to reagent requirements for xylene sub on the last two stations.? If you have any questions let me know. Pam Marcum UAMS ----- Original Message ----- From: "Karen - SMMC-SF Heckford" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 8, 2012 7:11:13 AM Subject: [Histonet] Tissue Processor I am going to need to purchase a new tissue processor mine keeps breaking down. ?What tissue processor would you buy and why? ?I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Caution: ?This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. ?The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. ?Any further review, dissemination, distribution, or copying of this message is strictly prohibited. ?If you have received this communication in error, please notify us ?immediately by reply email. ?Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Wed Aug 8 11:30:52 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Aug 8 11:30:57 2012 Subject: [Histonet] Ki67 antibody to stain Mouse tissue In-Reply-To: References: Message-ID: Eva, et al, Yes, the LabVision/ThermoSci rabbit polyclonal does stain mouse tissue. I've done validation trials on various species with the antibody and mouse was one of many that did react. Jan Shivers UMN Vet Diag Lab On Wed, Aug 8, 2012 at 10:51 AM, Eva Permaul wrote: > I just found a nice publication with the Rm-9106 on mouse intestine. Have > provided it to my manager. Thank you everyone. > Eva > > On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul wrote: > > > Just to clarify. Are you using it on mouse tissues? I am only asking > > because the Thermo Scientific/Labvision/Neomarkers data sheet says it has > > only been verified on Human tissues. > > Secondly had anyone used it on mouse gut. Does it stain in the bottom of > > the crypts or at the top? I am asking because I have also tried a > different > > antibody from Leica but got staining at the top of the crypts not at the > > bottom. > > > > Thank you to everyone for all of your valuable suggestions and advice, > > Eva Permaul > > Georgetown University > > > > > > On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) < > > joost.bruijntjes@tno.triskelion.nl> wrote: > > > >> Hi Eva > >> > >> I've used an antibody from Labvision /Neomarkers. It is a rabbit > >> monoclonal; catalog number: RM-9106. > >> > >> Joost > >> > >> -----Oorspronkelijk bericht----- > >> Van: histonet-bounces@lists.utsouthwestern.edu [mailto: > >> histonet-bounces@lists.utsouthwestern.edu] Namens Eva Permaul > >> Verzonden: maandag 6 augustus 2012 20:44 > >> Aan: histonet > >> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue > >> > >> Hello, > >> I am looking for a Ki67 to stain mouse tissues. We have been using Dako > >> M2749 but just found out it has been discontinued. Could anyone suggest > a > >> good alternative? > >> Thank you all for any help, > >> Eva Permaul > >> Georgetown University > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TMcNemar <@t> lmhealth.org Wed Aug 8 11:44:48 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Aug 8 11:45:01 2012 Subject: [Histonet] tissue highlighting for visibility In-Reply-To: <20120807181025.46opnxy2fok408sk@mail.histocare.com> References: <20120807181025.46opnxy2fok408sk@mail.histocare.com> Message-ID: We just add about 10-15 ml of eosin in the last alcohol on the processor Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of contact@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From timothy.m.coskran <@t> pfizer.com Wed Aug 8 12:10:20 2012 From: timothy.m.coskran <@t> pfizer.com (Coskran, Timothy M) Date: Wed Aug 8 12:10:24 2012 Subject: [Histonet] RE: Ki67 to stain mouse tissue Message-ID: <70249E5B79AFEB48A47D78568CE216E90847E0@NDHAMREXDE02.amer.pfizer.com> We've had good success with clone SP6, a rabbit monoclonal from Vector. This antibody works in many species and has been very reliable. Tim Coskran Pfizer From kiran_g <@t> sbcglobal.net Wed Aug 8 14:08:14 2012 From: kiran_g <@t> sbcglobal.net (kiran_g@sbcglobal.net) Date: Wed Aug 8 14:08:21 2012 Subject: [Histonet] RE: Ki67 to stain mouse tissue In-Reply-To: <70249E5B79AFEB48A47D78568CE216E90847E0@NDHAMREXDE02.amer.pfizer.com> References: <70249E5B79AFEB48A47D78568CE216E90847E0@NDHAMREXDE02.amer.pfizer.com> Message-ID: <1577397066-1344452895-cardhu_decombobulator_blackberry.rim.net-1448621264-@b11.c14.bise6.blackberry> Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Coskran, Timothy M" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 8 Aug 2012 17:10:20 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ki67 to stain mouse tissue We've had good success with clone SP6, a rabbit monoclonal from Vector. This antibody works in many species and has been very reliable. Tim Coskran Pfizer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MYang <@t> emc.org Wed Aug 8 14:22:05 2012 From: MYang <@t> emc.org (Yang, Mari) Date: Wed Aug 8 14:22:19 2012 Subject: [Histonet] Free-standing Dictation System for AP Message-ID: <1A53EC9F081AB34080CC7A602D4663DD89C302@NT106.info.sys> Dear Histonet members, Good afternoon! I was wondering if anyone can kindly recommend a free-standing electronic dictation system to be used with an Anatomic Pathology information systems? Any suggestions or advice would be greatly appreciated. Thank you, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From ratliffjack <@t> hotmail.com Thu Aug 9 08:11:27 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Aug 9 08:11:35 2012 Subject: [Histonet] NSH Hard Tissue Forum - August 18 in Bethesda, MD Message-ID: National Society for Histotechnology Hard Tissue Forum Event - Saturday, August 18, 2012 at the Doubletree by Hilton in Bethesda, MD! The Hard Tissue Committee of the National Society for Histotechnology is hosting it's 2012 Hard Tissue Forum Event in Bethesda, MD on August 18th! Registration is still open so come out a participate in this single day event that highlights microtomy equipment and techniques associated with the production of undemineralized bone and medical device implant histology. As a special treat, we are also hosting a first ever in the U.S. and N. America display and demonstration of new laser sectioning technology capable of non-contact cutting of fresh unprocessed and resin embedded tissues! This laser microtome or lasertome could represent the next evolutionary step in the advancement of histology and automation! Participants interested in a free, no obligation, private, hands-on demonstration before the event (August 15-17) are encouraged to contact me by reply email so I can help schedule your appointment! Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology From kenneth.metzger <@t> aruplab.com Thu Aug 9 09:49:28 2012 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Aug 9 09:51:01 2012 Subject: [Histonet] HT exam Message-ID: <3855827CD3E36249A30D57F6F896F8F19D2BE7@EXMBX2.aruplab.net> Can someone tell me what year they began requiring college credits for the HT exam? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From wbenton <@t> cua.md Thu Aug 9 10:01:31 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Aug 9 10:03:38 2012 Subject: [Histonet] RE: HT exam In-Reply-To: <3855827CD3E36249A30D57F6F896F8F19D2BE7@EXMBX2.aruplab.net> References: <3855827CD3E36249A30D57F6F896F8F19D2BE7@EXMBX2.aruplab.net> Message-ID: <0B8979A204680A42B93A52B486088CD92CCB563D77@CUAEXH1.GCU-MD.local> If you are referring to the removal of the OTJ portion of taking the registry with a H.S. diploma I think that happened sometime in the middle of 2000, maybe around 2004-2008. I don't recall the exact year. Check the ASCP website, it should be listed there or they should be able to answer that question. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth [kenneth.metzger@aruplab.com] Sent: Thursday, August 09, 2012 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Can someone tell me what year they began requiring college credits for the HT exam? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From JCBRITTON <@t> Cheshire-Med.COM Thu Aug 9 10:08:05 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Thu Aug 9 10:08:15 2012 Subject: [Histonet] RE: HT exam In-Reply-To: <0B8979A204680A42B93A52B486088CD92CCB563D77@CUAEXH1.GCU-MD.local> Message-ID: It was 2004! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Thursday, August 09, 2012 11:02 AM To: Metzger, Kenneth; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: HT exam If you are referring to the removal of the OTJ portion of taking the registry with a H.S. diploma I think that happened sometime in the middle of 2000, maybe around 2004-2008. I don't recall the exact year. Check the ASCP website, it should be listed there or they should be able to answer that question. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth [kenneth.metzger@aruplab.com] Sent: Thursday, August 09, 2012 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Can someone tell me what year they began requiring college credits for the HT exam? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCBRITTON <@t> Cheshire-Med.COM Thu Aug 9 10:08:46 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Thu Aug 9 10:08:59 2012 Subject: [Histonet] RE: HT exam In-Reply-To: <0B8979A204680A42B93A52B486088CD92CCB563D77@CUAEXH1.GCU-MD.local> Message-ID: It happened in 2004! Josie Britton HT (ASCP) Cheshire Medical Center 580 Court Street Keene, NH 03431 603-354-5454 ext. 2454 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Thursday, August 09, 2012 11:02 AM To: Metzger, Kenneth; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: HT exam If you are referring to the removal of the OTJ portion of taking the registry with a H.S. diploma I think that happened sometime in the middle of 2000, maybe around 2004-2008. I don't recall the exact year. Check the ASCP website, it should be listed there or they should be able to answer that question. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth [kenneth.metzger@aruplab.com] Sent: Thursday, August 09, 2012 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Can someone tell me what year they began requiring college credits for the HT exam? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Thu Aug 9 10:23:26 2012 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Aug 9 10:23:32 2012 Subject: [Histonet] Eosin Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ======================== ======= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ======================== ======= From sdysart <@t> mirnarx.com Thu Aug 9 10:27:30 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Aug 9 10:27:41 2012 Subject: [Histonet] RE: Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5018D553A1@BL2PRD0710MB363.namprd07.prod.outlook.com> Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ================ ===== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ================ ===== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Thu Aug 9 10:40:46 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Aug 9 10:40:57 2012 Subject: [Histonet] RE: Eosin In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5018D553A1@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03>, <8A70A9B2ECDD084DACFE6C59FCF86D5018D553A1@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF54843D@EXCHANGEMB1.hmc.hurleymc.com> I can still hear one of our pathologist's coming down the hall, loudly complaining that "SOMETHING'S WRONG WITH THE EOSIN!!!!!!" We added new, we changed to completely new, we played with the times.......all to no avail....... Then we changed the hematoxylin..............down the hall she came............"YOU FIXED THE EOSIN!!! THANK YOU!!" Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sarah Dysart [sdysart@mirnarx.com] Sent: Thursday, August 09, 2012 11:27 AM To: Hannen, Valerie; histonet Subject: [Histonet] RE: Eosin Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ================ ===== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ================ ===== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Thu Aug 9 10:57:03 2012 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Thu Aug 9 10:57:13 2012 Subject: [Histonet] Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> Message-ID: What clearing agent are you using? If it is a xylene substitute then you might want to rotate or change the alcohols after eosin after a few racks of slides. We use ProPar on our stainer and rotate our alcohols after eosin after every 3 racks that have gone through. On Thu, Aug 9, 2012 at 11:23 AM, Hannen, Valerie < Valerie.Hannen@parrishmed.com> wrote: > Hi Folks...I am hoping you all give me a little help. Our Pathologists > are complaining about our Eosin on the H&E's being weak. The funny thing > is, is that it can go one > > day to the next...one day it looks great...the next it is weak!! > > I have already done some experimenting with...1) time tissue spends in > Eosin.... 2) making sure that the alcohols after Eosin are the proper > concentrations....3) > > reducing the time that the tissues spends in the alcohols atfer Eosin... > I have even gone as far as 4) increasing the rinse time in water after the > decolorizing and bluing > > steps. 5) I have checked the pH of the water as well. > > Any help and suggestions would greatly appreciated!! > > Thanks Gang!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > > > > > > * > > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From b-frederick <@t> northwestern.edu Thu Aug 9 10:58:15 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Aug 9 10:58:23 2012 Subject: [Histonet] RE: Eosin In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5018D553A1@BL2PRD0710MB363.namprd07.prod.outlook.com> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> <8A70A9B2ECDD084DACFE6C59FCF86D5018D553A1@BL2PRD0710MB363.namprd07.prod.outlook.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1F9A2A61@evcspmbx2.ads.northwestern.edu> We add our own 1% phloxine in 95% alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, August 09, 2012 10:28 AM To: Hannen, Valerie; histonet Subject: [Histonet] RE: Eosin Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ================ ===== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ================ ===== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindy38017 <@t> yahoo.com Thu Aug 9 11:22:17 2012 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Thu Aug 9 11:22:24 2012 Subject: [Histonet] (no subject) Message-ID: <1344529337.55162.YahooMailNeo@web37106.mail.mud.yahoo.com> http://www.best-bridal.co.uk/wp-content/plugins/love.php?agree227.jpeg From mcauliff <@t> umdnj.edu Thu Aug 9 11:26:07 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 9 11:24:31 2012 Subject: [Histonet] Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> Message-ID: <5023E49F.1060702@umdnj.edu> Add a few drops of glacial acetic acid. Geoff On 8/9/2012 11:23 AM, Hannen, Valerie wrote: > Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one > > day to the next...one day it looks great...the next it is weak!! > > I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) > > reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing > > steps. 5) I have checked the pH of the water as well. > > Any help and suggestions would greatly appreciated!! > > Thanks Gang!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > > > > > > * > > ============================================== > ============= > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > ============================================== > ============= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Allison_Scott <@t> hchd.tmc.edu Thu Aug 9 12:48:10 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Aug 9 12:48:17 2012 Subject: [Histonet] Histology Laboratory Assistant Competency Message-ID: Hello to all in histoland. Does anyone have a histology lab assistant competency checklist that they would be willing to share. I am in need of one. Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Wanda.Smith <@t> HCAhealthcare.com Thu Aug 9 13:50:34 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Aug 9 13:50:48 2012 Subject: [Histonet] RE: Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA275B3FFB48@NADCWPMSGCMS03.hca.corpad.net> How about the thickness of the tissue? Once we were cutting skins at 3 microns and the pathologist complained the Eosin was too light so we started cutting them at 4-5 microns. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 11:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ================ ===== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ================ ===== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Thu Aug 9 14:13:58 2012 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Aug 9 14:14:06 2012 Subject: [Histonet] CAP autopsy requirement? Message-ID: <8CF447A43338C0F-1EA4-12F9@web-mmc-d06.sysops.aol.com> Am I hearing right? The CAP has done away with the requirement to sign out autopsies within 60 days? (But you still have to get the PAD out in two days)? How will I ever get pathologists motivated any more?! Michael Titford Pathology USA Mobile AL From mcauliff <@t> umdnj.edu Thu Aug 9 14:22:37 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 9 14:21:00 2012 Subject: [Histonet] RE: Eosin In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA275B3FFB48@NADCWPMSGCMS03.hca.corpad.net> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> <9E2D36CE2D7CBA4A94D9B22E8328A3BA275B3FFB48@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <50240DFD.2050905@umdnj.edu> Wanda makes an excellent point, thinner sections have less contrast. Geoff On 8/9/2012 2:50 PM, Wanda.Smith@HCAhealthcare.com wrote: > How about the thickness of the tissue? Once we were cutting skins at 3 microns and the pathologist complained the Eosin was too light so we started cutting them at 4-5 microns. > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > Sent: Thursday, August 09, 2012 11:23 AM > To: histonet > Subject: [Histonet] Eosin > > Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one > > day to the next...one day it looks great...the next it is weak!! > > I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) > > reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing > > steps. 5) I have checked the pH of the water as well. > > Any help and suggestions would greatly appreciated!! > > Thanks Gang!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > > > > > > * > > ================ > ===== > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > ================ > ===== > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Lynn.Burton <@t> Illinois.gov Thu Aug 9 14:46:03 2012 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Aug 9 14:48:00 2012 Subject: [Histonet] RE: Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C322E7D40E45@isexstore03> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC0065446A2E4@IL084EXMBX214.illinois.gov> We have used a mixture of Eosin Y and Phloxine B with acetic acid as follows: 780mL 95 %ETOH 100mL Eosin 10mL Phloxine B 4mL glacial acetic acid We seem to be consistent. We have used this for 17 years. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [Valerie.Hannen@parrishmed.com] Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ================ ===== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ================ ===== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Thu Aug 9 14:48:58 2012 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Thu Aug 9 14:49:32 2012 Subject: [Histonet] CAP autopsy requirement? In-Reply-To: <8CF447A43338C0F-1EA4-12F9@web-mmc-d06.sysops.aol.com> References: <8CF447A43338C0F-1EA4-12F9@web-mmc-d06.sysops.aol.com> Message-ID: If it's true, I hope our director doesn't share the information. We got the 60 day thing down pat and all of our TAT reports. Hate to see it all go to waste. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Thursday, August 09, 2012 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP autopsy requirement? Am I hearing right? The CAP has done away with the requirement to sign out autopsies within 60 days? (But you still have to get the PAD out in two days)? How will I ever get pathologists motivated any more?! Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Aug 9 15:00:25 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Aug 9 14:59:17 2012 Subject: [Histonet] CAP autopsy requirement? In-Reply-To: References: <8CF447A43338C0F-1EA4-12F9@web-mmc-d06.sysops.aol.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570712994568@smcmail02.somerset-healthcare.com> 90% of the cases need to be signed out in 60 days, not all cases. **NEW** 07/31/2012 ANP.33120 Final Report TAT Phase II The final autopsy report is produced within 60 working days in 90% of the cases. NOTE: The 90% threshold is used in recognition of the fact that occasional unusual cases may require more than 60 days for completion, particularly when external consultation is required. If cases exceed 60 days, there should be documentation of the reason for the delay and of ongoing review of this information by the director of the service. Evidence of Compliance: ? Review of turnaround time data for the final autopsy report REFERENCES 1) Adickes ED, Sims KL. Enhancing autopsy performance and reporting. A system for a 5-day completion time. Arch Pathol Lab Med. 1996;120:249-253 2) Smith MT, Garvin AJ. Anatomic pathology turnaround times. Use and abuse. Am J Clin Pathol 1996;106(Suppl 1):S70-S73 3) Baker PB, et al. Quality assurance of autopsy face sheet reporting, final autopsy report turnaround time, and autopsy rates. A College of American Pathologists Q-Probes study of 10 003 autopsies from 418 institutions. Arch Pathol Lab Med. 1996;120:1003-1008 4) Hanzlick RL. The autopsy lexicon. Suggested headings for the autopsy report. Arch Pathol Lab Med. 2000;124:594-603 5) Bove KE, et al. The role of the autopsy in medical malpractice cases, II. Controversy related to autopsy performance and reporting. Arch Pathol Lab Med. 2002;126:1032-1035 6) Hutchins GM, et al. Autopsy reporting. In: Collins KA, et al, eds. Autopsy Performance and Reporting. 2nd ed. Northfield, IL: College of American Pathologists: 2003; chap 28 7) Baker PB. Communication of autopsy results. In: Collins KA, et al, eds. Autopsy Performance and Reporting. 2nd ed. Northfield, IL: College of American Pathologists: 2003; chap 33 ** -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Thursday, August 09, 2012 3:49 PM To: 'mtitford@aol.com'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] CAP autopsy requirement? If it's true, I hope our director doesn't share the information. We got the 60 day thing down pat and all of our TAT reports. Hate to see it all go to waste. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Thursday, August 09, 2012 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP autopsy requirement? Am I hearing right? The CAP has done away with the requirement to sign out autopsies within 60 days? (But you still have to get the PAD out in two days)? How will I ever get pathologists motivated any more?! Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sadey <@t> hotmail.ca Thu Aug 9 16:34:06 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Thu Aug 9 16:34:10 2012 Subject: [Histonet] Processor Opinions pls Message-ID: Hello:We are looking for a new tissue processor. I've only ever used Sakura products but the technology of the Leica ASP6025 looks like very good technology.Has anyone used this processor? If so, can you give me your opinion?Thanks :)Sheila From lpwenk <@t> sbcglobal.net Thu Aug 9 17:08:11 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Aug 9 17:08:18 2012 Subject: [Histonet] HT exam In-Reply-To: <3855827CD3E36249A30D57F6F896F8F19D2BE7@EXMBX2.aruplab.net> References: <3855827CD3E36249A30D57F6F896F8F19D2BE7@EXMBX2.aruplab.net> Message-ID: <481C83CBBB574B53B28935FB8554E80B@HP2010> In the past, there were 3 routes (in short version): - minimum associate degree (or 60 credit hours) with 12 credit hours of biology and chemistry combined with 1 year on-the-job-training (OJT) - high school diploma plus 2 years OJT - completion of a NAACLS accredited histotechnology program As of January 2, 2005, the high school route was eliminated (so 2004 was the last year allowed for those with less than the associate degree/60 credit hours to take the high school route). The other 2 routes remain. FYI - ASCP, NSH and state histology societies started announcing/publishing this information non-stop for 5 years previous to the Jan 2005 date, that the high school route would be eliminated, leaving only the minimum associate degree and the NAACLS program routes. So this information was out there, that the HS route would be eliminated, starting in early 2000. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 Opinions expressed are mine, and do not reflect on the hospital. -----Original Message----- From: Metzger, Kenneth Sent: Thursday, August 09, 2012 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Can someone tell me what year they began requiring college credits for the HT exam? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Thu Aug 9 17:37:19 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Aug 9 17:37:21 2012 Subject: [Histonet] written procedures Message-ID: <50243B9F.9020606@umn.edu> Hello Histonetters, Would any of you be willing to share your SOP for changing your processors? I am in a research lab and although I keep track of how many smaples and how many runs I have done before I change or rotate I need a written procedure now. Currently I am using the VIP bench top processor. I am interested in procedures with and without the use of a hydrometer. Thanks in advance!! Colleen Forster U of MN From tony.henwood <@t> health.nsw.gov.au Thu Aug 9 18:10:38 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Aug 9 18:10:50 2012 Subject: [Histonet] Re: Teabags In-Reply-To: References: <9E4560B21D59954A9931FF35B7BCCDD4021A444967@EXCH01.wellspan.org> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D1CDF09@xmdb02.nch.kids> Agree totally Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, 8 August 2012 7:36 PM To: Clouse, Rosanna; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Teabags Hint when using these - do NOT try to fold them up into a nice looking square. Once processed and in paraffin, it is very difficult to find the edge, to try to open back up. Fold into a not nice to look at, off-set square that is slightly crumpled. Much easier to find the edge. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on Beaumont Hospital. -----Original Message----- From: Clouse, Rosanna Sent: Tuesday, August 07, 2012 3:31 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Teabags For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclouse@wellspan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From kim.tournear <@t> yahoo.com Thu Aug 9 20:46:47 2012 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Thu Aug 9 20:46:53 2012 Subject: [Histonet] Processor Opinions pls In-Reply-To: References: Message-ID: <3DD02646-EA33-41D4-92DD-5FF7C822F655@yahoo.com> Hi Sheila, I used to install these for Leica. It's a very good machine and processes tx nicely. I've put surgicals and bx's on the same run (8hr) during demo's and was amazed at the results. I definitely recommend you do a demo. Hope this helps. Sent from the iPhone of Kim Tournear ?? On Aug 9, 2012, at 4:34 PM, Sheila Adey wrote: > > Hello:We are looking for a new tissue processor. I've only ever used Sakura products but the technology of the Leica ASP6025 looks like very good technology.Has anyone used this processor? If so, can you give me your opinion?Thanks :)Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from the iPhone of Kim Tournear ?? On Aug 9, 2012, at 4:34 PM, Sheila Adey wrote: > > Hello:We are looking for a new tissue processor. I've only ever used Sakura products but the technology of the Leica ASP6025 looks like very good technology.Has anyone used this processor? If so, can you give me your opinion?Thanks :)Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From meghak <@t> g.clemson.edu Fri Aug 10 04:10:30 2012 From: meghak <@t> g.clemson.edu (Megha Kumar) Date: Fri Aug 10 04:10:40 2012 Subject: [Histonet] sections falling off in day 2 ISH Message-ID: Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * * * * From brendal.finlay <@t> medicalcenterclinic.com Fri Aug 10 07:00:40 2012 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Fri Aug 10 07:00:50 2012 Subject: [Histonet] Eosin Message-ID: <2a5dfbafb452b61eb013f6b5ce736c9f@medicalcenterclinic.com> Do you recycle your alcohols?? If you do and you put the recycled alcohols at the end (running down to xylene) it can GREATLY decolorize the eosin in the H&E stain.? Also, make sure you have a good rinse after the blueing step as that can mess with the eosin's pH which will also decrease staining. -----Original message----- From: "Hannen, Valerie" Valerie.Hannen@parrishmed.com Date: Thu, 09 Aug 2012 09:23:26 -0500 To: histonet histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin > Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one > > day to the next...one day it looks great...the next it is weak!! > > I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the properconcentrations....3) > > reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing > > steps. 5) I have checked the pH of the water as well. > > Any help and suggestions would greatly appreciated!! > > Thanks Gang!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > > > > > > * > > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidentialor otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Brendal C. Finlay, HT (ASCP) West Florida Medical Center Clinic brendal.finlay@medicalcenterclinic.com phone - 850.474.8758 fax - 850.474.8584 From patrick.lewis <@t> seattlechildrens.org Fri Aug 10 10:57:26 2012 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Aug 10 10:58:07 2012 Subject: [Histonet] Has anyone done ISH with fairly large FFPE sections? Message-ID: <3903BE18914F4440834F0E620415FFCA0BCE8307@PPWEXD01B.childrens.sea.kids> Hi Everyone, I am going to do some ISH for EBER on FFPE sections, and I have some concerns on the logistics. The kits I am looking at are for 50 rxns, and it seems like they expect to use 20 uL probe per rxn. My sections are rather large, covering about 2/3 of a standard slide. How many uL will it take to make sure I get good coverage and have enough to avoid drying out the slide? I plan to cover slip them and use a humidity chamber, but even still, I am worried that with such a small volume it don't be enough. These kits are expensive, +$900.00 each and if I have to double/triple my rxn volume that would use up the kit in a hurry. I haven't fully familiarized myself with the protocol yet, so maybe my concerns are unjustified, but if anyone has done some ISH, I'd appreciate any advice you'd care to give. Thanks Patrick. From fbozkurt <@t> gmail.com Fri Aug 10 12:24:36 2012 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Fri Aug 10 12:24:39 2012 Subject: [Histonet] keeping acids in the lab. Message-ID: Hello Histonet, How are you keeping daily used acids like hydrochloric, nitric, acetic and formic? Thank you.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 10 12:39:32 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 10 12:42:22 2012 Subject: [Histonet] RE: Has anyone done ISH with fairly large FFPE sections? In-Reply-To: <3903BE18914F4440834F0E620415FFCA0BCE8307@PPWEXD01B.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA0BCE8307@PPWEXD01B.childrens.sea.kids> Message-ID: We buy rubber cement from walmart or staples and seal the outside of the coverslip before the hybridization step. And we have had much success with minimal probe. But I understand, I cross my fingers when I do large resected pieces of liver. AND I keep on complaining to the vendors that they do not provide enough probe to cover paraffin sections of large size. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick [patrick.lewis@seattlechildrens.org] Sent: Friday, August 10, 2012 11:57 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Has anyone done ISH with fairly large FFPE sections? Hi Everyone, I am going to do some ISH for EBER on FFPE sections, and I have some concerns on the logistics. The kits I am looking at are for 50 rxns, and it seems like they expect to use 20 uL probe per rxn. My sections are rather large, covering about 2/3 of a standard slide. How many uL will it take to make sure I get good coverage and have enough to avoid drying out the slide? I plan to cover slip them and use a humidity chamber, but even still, I am worried that with such a small volume it don't be enough. These kits are expensive, +$900.00 each and if I have to double/triple my rxn volume that would use up the kit in a hurry. I haven't fully familiarized myself with the protocol yet, so maybe my concerns are unjustified, but if anyone has done some ISH, I'd appreciate any advice you'd care to give. Thanks Patrick. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Aug 10 13:01:36 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri Aug 10 13:01:42 2012 Subject: [Histonet] keeping acids in the lab. In-Reply-To: References: Message-ID: <090FA56107A969459F3941DDD5585C3A1184CA38@PHSX10MB10.partners.org> We, of course, keep them separate from bases. We don't have large amounts, so we keep them in cabinet under the hood. Do not keep in flammable cabinets. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Friday, August 10, 2012 1:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] keeping acids in the lab. Hello Histonet, How are you keeping daily used acids like hydrochloric, nitric, acetic and formic? Thank you.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From tthinnes <@t> scripps.edu Fri Aug 10 13:05:10 2012 From: tthinnes <@t> scripps.edu (Therese C. Thinnes) Date: Fri Aug 10 13:05:15 2012 Subject: [Histonet] sections falling off in day 2 ISH (Megha Kumar) Message-ID: <1429E91BEE8A1F48AB0B6FA15624676F3767A4CCE9@EXCH-CCR01.lj.ad.scripps.edu> Hi Megha, Not sure why your sections are falling off. When I did ISH I used Colorfrost Plus Slides. I would cut at 4 microns-mouse or human. I would dry them at room temp a few hours followed by 55 C for an hour. I would use the slides the next day or next week, never the same day. On day 2 my SSC was 60 C and I used a stir bar for 2 hours on slow stirring. Rarely did I lose any sections. Terri Thinnes Research Assistant The Scripps Research Institute La Jolla, CA Message: 13 Date: Fri, 10 Aug 2012 14:40:30 +0530 From: Megha Kumar Subject: [Histonet] sections falling off in day 2 ISH To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * From patrick.lewis <@t> seattlechildrens.org Fri Aug 10 15:46:56 2012 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Aug 10 15:47:07 2012 Subject: [Histonet] Preferences between kits for EBER Message-ID: <3903BE18914F4440834F0E620415FFCA0BCE835C@PPWEXD01B.childrens.sea.kids> Hi Everyone, Thanks for the response earlier. Does anyone any advice on EBER ISH Kits? Specifically, if they had better results, or less problems with one brand vs another? Right now I am considering both Novacastra's kit for 970.00 and Vector Labs kit for 905.00 Has anyone used these kits? Has anyone tried a different kit they could recommend? Thanks Patrick. From jmacdonald <@t> mtsac.edu Fri Aug 10 20:21:36 2012 From: jmacdonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Aug 10 20:21:49 2012 Subject: [Histonet] Preferences between kits for EBER In-Reply-To: <3903BE18914F4440834F0E620415FFCA0BCE835C@PPWEXD01B.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA0BCE835C@PPWEXD01B.childrens.sea.kids> Message-ID: <96113846-2C1A-4151-99E5-3FDD6F51BDBB@mtsac.edu> Biocare has a kit. My class used it successfully. On Aug 10, 2012, at 1:46 PM, "Lewis, Patrick" wrote: > Hi Everyone, > > Thanks for the response earlier. > > Does anyone any advice on EBER ISH Kits? Specifically, if they had better results, or less problems with one brand vs another? > Right now I am considering both Novacastra's kit for 970.00 and Vector Labs kit for 905.00 > Has anyone used these kits? Has anyone tried a different kit they could recommend? > > Thanks > > Patrick. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge <@t> aol.com Fri Aug 10 22:43:42 2012 From: djemge <@t> aol.com (Donna) Date: Fri Aug 10 22:39:47 2012 Subject: [Histonet] Histology Technologist 2, new position at Northwestern University, Chicago Campus Message-ID: <025c01cd7773$8120fea0$8362fbe0$@com> Job Title: Histology Technologist 2 Job ID: 19392 Department: Cancer Center Northwestern University, Chicago Campus This is a newly created position in a small Core Laboratory that offers histology service University wide for both campuses. Apply online by following the information and link below. This is a great position located in the heart of beautiful downtown Chicago. Apply on the Northwestern University Careers site by going to: http://www.northwestern.edu/hr/jobs From gu.lang <@t> gmx.at Sun Aug 12 09:42:47 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Aug 12 09:42:55 2012 Subject: AW: [Histonet] sections falling off in day 2 ISH In-Reply-To: References: Message-ID: <001801cd7898$be193ea0$3a4bbbe0$@gmx.at> Hi I do FISH on 2-3 ?m human paraffin sections. They are mounted on Superfrost Plus slides, dried at least over night in an 56?C oven, sometimes over the weekend without negative influence. Hybridization is done in a Hybridizer, coverglass sealed with rubbergum over night. Next day I put away the rubbergum and the coverglass with care, just lifting the coverglass with a scalpell or needle. Then put the slides directly in the prewarmed stringent-wash-buffer (here 55?C 5 min), rinse in dest. water, let airdry and coverslip with DAPI contenting mounting medium. You may compare your protocol to mine. We hardly had problems with falling off sections. One time we even had success to stain a section on a "normal" glass slide, but treated it like a raw egg. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Megha Kumar Gesendet: Freitag, 10. August 2012 11:11 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] sections falling off in day 2 ISH Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * * * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Cremer <@t> med.kuleuven.be Mon Aug 13 02:12:10 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Mon Aug 13 02:12:27 2012 Subject: [Histonet] keeping acids in the lab. In-Reply-To: References: Message-ID: We keep them in a ventilated cabinet next to the fume hood, but they're 1 l bottles. You should not put them together with bases, organic solvents or other chemicals. Also, put the bottles in a dish that is resistant to the acids (glass is very resistant, but more prone to breakage than plastics) and which can contain the entire volume of the recipients. Certain acids are incompatible with each other as well; e.g. perchloric acid, which should normally be treated as if it's Hannibal Lecter. Of course, this is al in a perfect world with no money and space constraints... Using common sense and keeping only small volumes already goes a long way. --- Jonathan Cremer Laboratory Technician ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Mehmet Fatih BOZKURT [fbozkurt@gmail.com] Verzonden: vrijdag 10 augustus 2012 19:24 To: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] keeping acids in the lab. Hello Histonet, How are you keeping daily used acids like hydrochloric, nitric, acetic and formic? Thank you.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joost.bruijntjes <@t> tno.triskelion.nl Mon Aug 13 03:17:58 2012 From: joost.bruijntjes <@t> tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Mon Aug 13 03:18:05 2012 Subject: [Histonet] lipid peroxidase activity Message-ID: Hi histonetters Is anyone of you familiar with the histochemical staining of lipid peroxidase activity in liver? If so, would you be so kind to join your protocol with me? Joost Bruijntjes TNO_triskelion The Netherlands From kandrac2 <@t> gmail.com Mon Aug 13 05:49:58 2012 From: kandrac2 <@t> gmail.com (=?ISO-8859-2?Q?Ondrej_Kandr=E1=E8?=) Date: Mon Aug 13 05:50:02 2012 Subject: [Histonet] aged dog brain immunohistochemistry Message-ID: Good afternoon, has anyone experiences with immunohistochemistry (IHC) of *aged dog brain*fixen in paraffin? Thank you! -- Ondrej Kandr?? Research trainee Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia From rsrichmond <@t> gmail.com Mon Aug 13 12:37:28 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Aug 13 12:37:32 2012 Subject: [Histonet] Re: keeping acids in the lab. Message-ID: I was recently surprised to learn that glacial acetic acid is more of a fire hazard than it is a corrosive. I had no idea it would burn. Handling hazmats actually requirs some knowledge of chemistry, not a work-to-rule like the bureaucrats' fond dreams. Bob Richmond Samurai Pathologist Maryville TN From Laurie <@t> blufrogpath.com Mon Aug 13 16:35:22 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Mon Aug 13 16:35:28 2012 Subject: [Histonet] Special Stain documentation Message-ID: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert From Joyce.Weems <@t> emoryhealthcare.org Mon Aug 13 17:06:13 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Aug 13 17:06:28 2012 Subject: [Histonet] Special Stain documentation In-Reply-To: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> References: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> Message-ID: Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mw <@t> personifysearch.com Tue Aug 14 07:27:53 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Aug 14 07:27:59 2012 Subject: [Histonet] Now Hiring - Field Applications Support - Northeast Message-ID: <541bcab61f44d7ed6ce913b3128b7954@mail.gmail.com> Good morning Histonet, We are currently searching for a talented histology professional with a strong background in routine histology to join our clients field support team in the Northeast. The right candidate can be based near a major airport and must be open to travel. The position offers an outstanding opportunity to break into industry, a very competitive package, and excellent growth potential. If this sounds like something you would like to learn more about please send me a copy of your resume to mw@personifysearch.com or call me at 800.875.6188 ext. 103. Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From ChaseM <@t> childrensdayton.org Tue Aug 14 08:10:04 2012 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Tue Aug 14 08:10:08 2012 Subject: [Histonet] Special Stain documentation In-Reply-To: References: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> Message-ID: <187FB57B597CB24F9F59F357264999F715AFC9DB@PEXCHNGDAG01.cmc-dayton.org> We have this statement embedded into every report: "Quality of hematoxylin and eosin staining was appropriate for slide evaluation". and if it's an immuno we have an auto text with the same type of wording. We first tried to get the pathologists to sign a log each day but that was a nightmare. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, August 13, 2012 6:06 PM To: 'Laurie@blufrogpath.com'; Histonet post Subject: RE: [Histonet] Special Stain documentation Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. From relia1 <@t> earthlink.net Tue Aug 14 08:59:06 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Aug 14 08:59:13 2012 Subject: [Histonet] CAP education requirements for grossing Message-ID: <009101cd7a24$f92d9da0$eb88d8e0$@earthlink.net> Hi Fellow Histonetters!! I hope everyone is having a great day!! I have a quick question from a client that I hope someone can give me a definitive answer on. What are the education requirements for grossing under CAP as opposed to CLIA. I was told that for CAP a B.S. degree is required and I wanted to check and see if anyone knew if that was the case. I know CLIA is an A. S. Degree or a certain number of hours in biology. Does CAP require a bachelor's of science? Thanks-Pam Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From one_angel_secret <@t> yahoo.com Tue Aug 14 09:33:27 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Aug 14 09:33:32 2012 Subject: [Histonet] CAP education requirements for grossing In-Reply-To: <009101cd7a24$f92d9da0$eb88d8e0$@earthlink.net> References: <009101cd7a24$f92d9da0$eb88d8e0$@earthlink.net> Message-ID: <1344954807.91626.YahooMailNeo@web112304.mail.gq1.yahoo.com> Unless its changed from CAP 2011 checklist and I didnt hear about it. Grossing is considered high complexity, so the guidelines are the same for anyone doing high complexity testing. They would need documented training as do any other high comlexity task. ? here is what CAP 2011 says about it. ?**REVISED** 07/11/2011ANP.11610 Gross Examination Qualifications Phase IIIf individuals other than a pathologist or pathology resident assist in gross examinations, such individuals qualify as high complexity testing personnel under CLIA regulations.NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist requirement. This checklist requirement applies only to laboratories subject to US regulations.Evidence of Compliance:? Records of qualifications including degree or transcript and work history in related field ORdocumentation of grandfathered exceptionREFERENCES 1) of 1988; final rule. Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendmentsFed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072 [42CFR493.1491]ANP.11640 Performance Evaluation Phase IIThe performance of non-pathologist(s) who assist in the performance of gross tissue examinations is evaluated by the pathologist on a regular, periodic basis.Evidence of Compliance:? Written procedure and schedule for evaluating performance of non-pathologists AND? Records of evaluation documented at defined frequencyREFERENCES 1) Cibull ML. Q&A. Northfield, IL: College of American Pathologists CAP Today. 1997;11(7):1122) Grzybicki DM, et al. The usefulness of pathologists' assistants. Am J Clin Pathol. 1999;112:619-626 ? ________________________________ From: Pam Barker To: Histonet Sent: Tuesday, August 14, 2012 9:59 AM Subject: [Histonet] CAP education requirements for grossing Hi Fellow Histonetters!! I hope everyone is having a great day!!? I have a quick question from a client that I hope someone can give me a definitive answer on.? What are the education requirements for grossing under CAP as opposed to CLIA.? I was told that for CAP a B.S. degree is required and I wanted to check and see if anyone knew if that was the case.? I know CLIA is an A. S. Degree or a certain number of hours in biology.? Does CAP require a bachelor's of science?? Thanks-Pam? Thank You! ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ? (407)353-5070 FAX:? ? (407)678-2788 E-mail: relia1@earthlink.net http://www.facebook.com/ /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Aug 14 09:51:49 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Aug 14 09:51:54 2012 Subject: [Histonet] RELIA Solutions Histology Opportunities Update 8/14/2012. Exciting New Opportunities with Great companies and Brand New Labs in FL, NC, PA and IL Message-ID: <00ac01cd7a2c$568f1620$03ad4260$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day and staying cool through these dog days of summer. It is really hot and humid down here in Florida and the only thing hotter than the temps is these sizzling new positions I am working on. All of these are full time permanent positions with BRAND NEW LABS!!! These are exclusive RELIA opportunities. My clients offer a competitive pay, great benefits, relocation assistance and they are ready to hire right away!! Here is a list of the positions I am talking about: Histology Supervisor - Philadelphia area Histotechnologist - Philadelphia area Lead Tech Dermpath/Grossing - Reading, PA Histology Supervisor - Daytona Beach, FL Histotechnician/Histotechnologist - Charlotte, NC Lead Histotech - Belleville, IL If you or anyone you know might be interested in any of these positions please contact me right away. ALL of these positions are with Brand New Labs and RELIA has the exclusive. I would love to tell you about any of these opportunities. If you are interested in looking at positions in other areas then we need to connect too. I am getting all of these great opportunities on an almost daily basis Nationwide!! Remember I also will pay you a referral fee if I place someone that you refer to me. Thanks-Pam Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Nancy_Schmitt <@t> pa-ucl.com Tue Aug 14 12:15:49 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Aug 14 12:15:35 2012 Subject: [Histonet] Special Stain documentation In-Reply-To: <20120814170526.C91CC1AA036@mail.pa-ucl.com> References: <20120814170526.C91CC1AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C362209FCD0@PEITHA.wad.pa-ucl.com> Laurie- Our Histotechs document on a stain log that is printed, and the pathologists comment "controls stained appropriately" within the report. Nancy ----- Message: 2 Date: Mon, 13 Aug 2012 14:35:22 -0700 From: Subject: [Histonet] Special Stain documentation To: "Histonet post" Message-ID: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> Content-Type: text/plain; charset="utf-8" How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert ------------------------------ Message: 3 Date: Mon, 13 Aug 2012 22:06:13 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] Special Stain documentation To: "'Laurie@blufrogpath.com'" , Histonet post Message-ID: Content-Type: text/plain; charset="utf-8" Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From pruegg <@t> ihctech.net Tue Aug 14 12:27:22 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Aug 14 12:27:27 2012 Subject: [Histonet] sections falling off in day 2 ISH (Megha Kumar) In-Reply-To: <1429E91BEE8A1F48AB0B6FA15624676F3767A4CCE9@EXCH-CCR01.lj.ad.scripps.edu> References: <1429E91BEE8A1F48AB0B6FA15624676F3767A4CCE9@EXCH-CCR01.lj.ad.scripps.edu> Message-ID: <8625B5CEA8C4402FB2E4641708D17C73@DESKTOP3> I would use good charged slides like VWR Superfrost Plus or Fisher + slides and try to cut the sections thinner, sections thicker than 4-6 microns fall off easier. It is also important to airdry slides completely before baking them, we bake at 60dc for an hour to overnight in an oven after airdrying. For ish we do it manually on a hot plate and put a glass coverslip over the section to keep it from drying out during high temp long incubations required for ISH. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therese C. Thinnes Sent: Friday, August 10, 2012 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections falling off in day 2 ISH (Megha Kumar) Hi Megha, Not sure why your sections are falling off. When I did ISH I used Colorfrost Plus Slides. I would cut at 4 microns-mouse or human. I would dry them at room temp a few hours followed by 55 C for an hour. I would use the slides the next day or next week, never the same day. On day 2 my SSC was 60 C and I used a stir bar for 2 hours on slow stirring. Rarely did I lose any sections. Terri Thinnes Research Assistant The Scripps Research Institute La Jolla, CA Message: 13 Date: Fri, 10 Aug 2012 14:40:30 +0530 From: Megha Kumar Subject: [Histonet] sections falling off in day 2 ISH To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Aug 14 12:30:16 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Aug 14 12:30:35 2012 Subject: [Histonet] Has anyone done ISH with fairly large FFPE sections? In-Reply-To: <3903BE18914F4440834F0E620415FFCA0BCE8307@PPWEXD01B.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA0BCE8307@PPWEXD01B.childrens.sea.kids> Message-ID: <561173CE85EE421E8770E01F33FC455D@DESKTOP3> We use a Tunnel kit from Chemicon and apply about 30ul of probe with a cs on top, look into buying just the probe which is what goes first, for our Chemicon kit we buy several vials of probe before we have to buy the entire kit again. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, August 10, 2012 9:57 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Has anyone done ISH with fairly large FFPE sections? Hi Everyone, I am going to do some ISH for EBER on FFPE sections, and I have some concerns on the logistics. The kits I am looking at are for 50 rxns, and it seems like they expect to use 20 uL probe per rxn. My sections are rather large, covering about 2/3 of a standard slide. How many uL will it take to make sure I get good coverage and have enough to avoid drying out the slide? I plan to cover slip them and use a humidity chamber, but even still, I am worried that with such a small volume it don't be enough. These kits are expensive, +$900.00 each and if I have to double/triple my rxn volume that would use up the kit in a hurry. I haven't fully familiarized myself with the protocol yet, so maybe my concerns are unjustified, but if anyone has done some ISH, I'd appreciate any advice you'd care to give. Thanks Patrick. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Norm.Burnham <@t> propath.com Tue Aug 14 15:11:49 2012 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Tue Aug 14 15:11:53 2012 Subject: [Histonet] Best Group Purchasing Organization (GPO) for clinical and pathology laboratories? Message-ID: <82C7248978CB50469FD6BA68EBBEFE67085C869E@exchange.propathlab.com> Dear Histonetters, We are currently searching for the most inclusive and most cost effective lab-oriented Group Purchasing Organization (GPO). Are you working with one that gives great support and greatest discounts on lab general supplies and if so, may I ask who you are using? I am sure that there are many of us out here who would like to know which GPO's are "best of breed" for lab purchases and greatest savings. I would be so appreciative to hear your thoughts. Thank you and best regards. Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath Dallas, TX 75247 www.propath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From TNMayer <@t> mdanderson.org Tue Aug 14 15:28:08 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Aug 14 15:28:40 2012 Subject: [Histonet] RE: Special Stain documentation Message-ID: I have seen a phrase in a lot of reports that states: "All controls are appropriate," or something to that effect. I explain that it is a legally binding phrase, that is on reports by the pathologists to verify the control is correct. I have always heard that if the case went to court, the pathologist could be asked to testify to that fact. The techs also document that the control worked on the log sheet. Toysha Mayer ------------------------------ Message: 2 Date: Mon, 13 Aug 2012 14:35:22 -0700 From: Subject: [Histonet] Special Stain documentation To: "Histonet post" Message-ID: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> Content-Type: text/plain; charset="utf-8" How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert ------------------------------ Message: 3 Date: Mon, 13 Aug 2012 22:06:13 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] Special Stain documentation To: "'Laurie@blufrogpath.com'" , Histonet post Message-ID: Content-Type: text/plain; charset="utf-8" Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert ------------------------------ ________________________________ ------------------------------ From richard.wild <@t> wanadoo.fr Wed Aug 15 01:45:21 2012 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Wed Aug 15 01:45:22 2012 Subject: [Histonet] Help ! autostainer plus - lost name and password Message-ID: <502B4581.6080006@wanadoo.fr> Hello I just bought a second hand Dako autostainer from Germany (I live in France) from a broker. The machine looks fine but I can't test it because_I don't have identity and password _to get inside the software. Does anyone know how if it is possible to localise the password and identities in the hard disk (some "ini" file ? by the regedit ? ... but where ?) ? Or is it possible to destroy a file, so that the software begins a new cycle asking password and all ? Or is there any other procedure to get through ? DAKO autostainer plus or LABVISION 480 could use similar software. Best friendly regards. Richard From tgenade <@t> gmail.com Wed Aug 15 07:44:09 2012 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Wed Aug 15 07:44:13 2012 Subject: [Histonet] titron vs DMSO Message-ID: Hello, I suspect that 0.1% triton X-100 in my blocking solution may be causing my frozen (unfixed) sections of brain to come off the slide. These are APTES slides and the sections were 20 and 40 micron thick. At first it looked to me that the entire section had come off but under the microscope I could see a thin layer (of single?) cells still stuck to the slide. There was also a chance in antibody staining. The antiserum I am using normally gives very defined intense staining of a surface antigen but now the staining was punctate. I suspect the triton is too strong and as well as washing off the tissue it is also disrupting the membranes and interfering with the staining. I have not seen this when I was using the same blocking solution without the triton X-100. For wholemounts I had used DMSO for permeabilization but also have NP-40 available. Does anyone have an opinion on the matter? -- Tyrone Genade Dept. Human Biology University of Cape Town From rjbuesa <@t> yahoo.com Wed Aug 15 08:30:30 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 15 08:30:34 2012 Subject: [Histonet] titron vs DMSO In-Reply-To: References: Message-ID: <1345037430.66759.YahooMailNeo@web121406.mail.ne1.yahoo.com> Triton is a surfactant used to facilitate the dispersion of ?IHC reagents but if you say that without Triton the sections did not fall you have the solution. Try sections with/without Triton, evaluate the results and act accordingly. Ren? J. ________________________________ From: Tyrone Genade To: histonet Sent: Wednesday, August 15, 2012 8:44 AM Subject: [Histonet] titron vs DMSO Hello, I suspect that 0.1% triton X-100 in my blocking solution may be causing my frozen (unfixed) sections of brain to come off the slide. These are APTES slides and the sections were 20 and 40 micron thick. At first it looked to me that the entire section had come off but under the microscope I could see a thin layer (of single?) cells still stuck to the slide. There was also a chance in antibody staining. The antiserum I am using normally gives very defined intense staining of a surface antigen but now the staining was punctate. I suspect the triton is too strong and as well as washing off the tissue it is also disrupting the membranes and interfering with the staining. I have not seen this when I was using the same blocking solution without the triton X-100. For wholemounts I had used DMSO for permeabilization but also have NP-40 available. Does anyone have an opinion on the matter? -- Tyrone Genade Dept. Human Biology University of Cape Town _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Aug 15 09:07:05 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 15 09:07:08 2012 Subject: [Histonet] Brand New Lab in Charlotte!! Excellent Pay, Great Benefits and Relocation Assistance - An Awesome Opportunity from RELIA Solutions!! Message-ID: <007b01cd7aef$406892e0$c139b8a0$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day. So from what I hear Charlotte, NC is a really beautiful city and a lot of people would love to live and work there. My question is Are you one of those people OR do you know someone who is? The reason I am asking is that I have a brand new lab in Charlotte that I am currently recruiting for. We need several histology techs and a grossing tech. They are interested in experienced techs who are ASCP certified or eligible and have an A.S. degree. This is an established facility moving into a brand new lab. I know several people who work there and they love it. Everyone that I have dealt with there has been absolutely wonderful. This client has absolutely no qualms about paying interview expenses and relocation assistance. The priority for them is getting the right people in their lab. I LOVE THESE GUYS!!! These are full time permanent positions and my client offers excellent pay, outstanding benefits and relocation assistance. If you or anyone you know might be interested in hearing more about this opportunity please contact me ASAP!! I can be reached by email at relia1@earthlink.net or toll free at 866-607-3542 until 5p EST or anytime on my cell phone 407-353-5070. Remember it never Hurts to Look! Thanks-Pam Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From MITCHELLJA <@t> email.chop.edu Wed Aug 15 09:25:05 2012 From: MITCHELLJA <@t> email.chop.edu (Mitchell, Janice A) Date: Wed Aug 15 09:25:32 2012 Subject: [Histonet] Slide printing systems Message-ID: Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) From TMcNemar <@t> lmhealth.org Wed Aug 15 10:02:56 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Aug 15 10:03:04 2012 Subject: [Histonet] Two identifiers... Message-ID: Hello all, For those of you using cassette and slide labelers.... Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From 1949rlh <@t> gmail.com Wed Aug 15 10:21:32 2012 From: 1949rlh <@t> gmail.com (=?utf-8?B?MTk0OXJsaEBnbWFpbC5jb20=?=) Date: Wed Aug 15 10:21:39 2012 Subject: [Histonet] (no subject) Message-ID: <502bbe7e.2528b60a.30c1.46ae@mx.google.com> I am trying to locate Paul Johnson Anatomic Consultant Knoxville, TN area. Thanks for your help. Rita Humphrey 205-532-4152 Sent from my Verizon Wireless Phone From Pat.Bell <@t> ucdenver.edu Wed Aug 15 10:25:41 2012 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Aug 15 10:26:34 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: References: Message-ID: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt> I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Aug 15 10:28:52 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Aug 15 10:28:58 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt> Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> We had the slide mate and we do not use it any more, its worthless. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 15, 2012 9:26 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Wed Aug 15 10:30:22 2012 From: cforster <@t> umn.edu (Colleen Forster) Date: Wed Aug 15 10:30:29 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt> References: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt> Message-ID: <502BC08E.8050408@umn.edu> Try Dako, they have a nice little slide printer out there now..... Colleen Forster U of MN On 8/15/2012 10:25 AM, Bell, Pat wrote: > I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. > > Pat Bell HT(ASCP) > University of Colorado, Denver > Medical Oncology; MS 8117 > 12801 E 17th Ave. > RC1-S, L18-8402C > Aurora, Co. 80045 > 303-724-6077 > pat.bell@ucdenver.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A > Sent: Wednesday, August 15, 2012 8:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide printing systems > > Good Morning, > > We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. > > I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. > > Thanks, Janice > > Janice A. Mitchell, BS, HT(ASCP) > Histology Lead Tech > Children's Hospital of Philadelphia > Anatomic Pathology and Laboratory Medicine > 324 S. 34th Street > Philadelphia, Pa 19104-4399 > 215-590-1738(lab) > 267-426-7754(office) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brannon <@t> alliedsearchpartners.com Wed Aug 15 10:37:07 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Aug 15 10:37:27 2012 Subject: [Histonet] Job Alert: Histology Supervisor- Kentwood, MI Message-ID: Allied Search Partners is working with an outstanding organization near Grand Rapids, MI to find a competent Histology Supervisor. Job Title: Histology Supervisor Location: Kentwood, MI area To apply: Please send resume to Brannon@alliedsearchpartners.com or fax to 888 388 7572. Please not that no other information can be given until we receive your resume. No resume will be submitted to current or previous employers. Schedule: This is a full time (40 hrs/week) Monday-Friday Day Shift. Working every 4th Saturday. Job Summary: The Histology Supervisor is responsible for the preparation of tissue specimens for microscopic evaluation by the pathologist by means of tissue sampling, processing, embedding, microtomy, routine staining, and diagnostic special staining. This person should also be experienced with gross room tissue sampling. In addition to these responsibilities, the supervisor is in charge of the daily operation of the Histology Lab. The supervisor typically works 3-4 days/week on the bench and 1-2 days/week supervisory. Experience/Education: Previous histology supervisory experience is required and should include IHC experience. Bachelor of Science Degree and HTL preferred but not required. Successful completion of an approved ASCP certified Histology program and Board of Registry Examination. Certification must be through the American Society of Clinical Pathologists or equivalent. -- Brannon Owens Recruitment Manager Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 From mpence <@t> grhs.net Wed Aug 15 10:38:07 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Aug 15 10:38:16 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E89@is-e2k3.grhs.net> They just came and worked on our Printmate and did an "update" "upgrade" for us to fix all the problems we have been having with print quality. So far so good 8 weeks and counting. You might ask your rep. about this. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 15, 2012 10:26 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Aug 15 10:41:58 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 15 10:42:11 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> References: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt> <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3AC4@Mail2Node1.ad.uams.edu> We have twelve, scattered through three departments and have had no issues. Early models had a print head issue that was resolved. We wouldn't trade them for any other system on the market today. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, August 15, 2012 10:29 AM To: 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems We had the slide mate and we do not use it any more, its worthless. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 15, 2012 9:26 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PAMarcum <@t> uams.edu Wed Aug 15 10:44:50 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 15 10:45:56 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3AE7@Mail2Node1.ad.uams.edu> We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, August 15, 2012 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two identifiers... Hello all, For those of you using cassette and slide labelers.... Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mpence <@t> grhs.net Wed Aug 15 10:59:04 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Aug 15 10:59:08 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3AE7@Mail2Node1.ad.uams.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974E8A@is-e2k3.grhs.net> What check list question states that there "must be two identifiers" on the slide and the cassette? I don't see it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Wednesday, August 15, 2012 10:45 AM To: 'Tom McNemar'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Two identifiers... We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, August 15, 2012 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two identifiers... Hello all, For those of you using cassette and slide labelers.... Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Wed Aug 15 11:03:15 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Aug 15 11:03:20 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> References: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt>, <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> Message-ID: <0B8979A204680A42B93A52B486088CD92CCB563DAB@CUAEXH1.GCU-MD.local> I too have the same systems that are being referenced. The Printmate did have a ton of problems with the original print heads, however they are doing a sweep to replace all of them according to my rep. We no longer experience the broken pins or other associated issues with the Print Mate. As for the slide mate, we had similar issues with that as well and found that after it was serviced a second time that we needed upgraded software/firmware on the instrument itself. Another issue that goes untouched by many users is the advanced user menu, which controls the pressure in which the thermal head presses on the slides and a variety of other attributes. Most reps are not aware of these features and thus are unable to help resolve your issues. Once we found out about these settings our slidemates work great. In addition to the advance settings on the slide mate it is important to wipe the thermal print head with an alcohol wipe daily to keep it clean performing optimally. Another issue that I have witnessed is that some of the paint on some slides do not work as well as others. I recently tested a slide with paint specifically formulated for the slide mate and the print was the best I have ever seen. Overall the units work well provided they are maintained and you have a knowledgeable support person to help you test out some of the advance menu features. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala [liz@premierlab.com] Sent: Wednesday, August 15, 2012 11:28 AM To: 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems We had the slide mate and we do not use it any more, its worthless. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 15, 2012 9:26 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From JEllin <@t> yumaregional.org Wed Aug 15 11:07:59 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Aug 15 11:08:07 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: References: Message-ID: Have you looked at the type of slides you are using? We have both instruments as we do have challenges with printing in the beginning , we now do not have issues -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From PAMarcum <@t> uams.edu Wed Aug 15 11:09:44 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 15 11:09:51 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974E8A@is-e2k3.grhs.net> References: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3AE7@Mail2Node1.ad.uams.edu> <661949901A768E4F9CC16D8AF8F2838C03974E8A@is-e2k3.grhs.net> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B19@Mail2Node1.ad.uams.edu> UAMS refers to it as HIPPA requirement and when we were inspected it was commented was a great cross check. Pam -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, August 15, 2012 10:59 AM To: Marcum, Pamela A; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... What check list question states that there "must be two identifiers" on the slide and the cassette? I don't see it! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Wednesday, August 15, 2012 10:45 AM To: 'Tom McNemar'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Two identifiers... We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, August 15, 2012 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two identifiers... Hello all, For those of you using cassette and slide labelers.... Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From cbrya <@t> lexclin.com Wed Aug 15 11:15:05 2012 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Aug 15 11:15:09 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <0B8979A204680A42B93A52B486088CD92CCB563DAB@CUAEXH1.GCU-MD.local> References: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt>, <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> <0B8979A204680A42B93A52B486088CD92CCB563DAB@CUAEXH1.GCU-MD.local> Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4174054559@EXCHANGESB> We are getting the PrintMate and SlideMate for our lab very soon. What brand of slides are you using that works best? Are they white slides or colored? Thank you in advance for your input. Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, August 15, 2012 12:03 PM To: Elizabeth Chlipala; 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I too have the same systems that are being referenced. The Printmate did have a ton of problems with the original print heads, however they are doing a sweep to replace all of them according to my rep. We no longer experience the broken pins or other associated issues with the Print Mate. As for the slide mate, we had similar issues with that as well and found that after it was serviced a second time that we needed upgraded software/firmware on the instrument itself. Another issue that goes untouched by many users is the advanced user menu, which controls the pressure in which the thermal head presses on the slides and a variety of other attributes. Most reps are not aware of these features and thus are unable to help resolve your issues. Once we found out about these settings our slidemates work great. In addition to the advance settings on the slide mate it is important to wipe the thermal print head with an alcohol wipe daily to keep it clean performing optimally. Another issue that I have witnessed is that some of the paint on some slides do not work as well as others. I recently tested a slide with paint specifically formulated for the slide mate and the print was the best I have ever seen. Overall the units work well provided they are maintained and you have a knowledgeable support person to help you test out some of the advance menu features. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala [liz@premierlab.com] Sent: Wednesday, August 15, 2012 11:28 AM To: 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems We had the slide mate and we do not use it any more, its worthless. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 15, 2012 9:26 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From pathrm35 <@t> comcast.net Wed Aug 15 11:38:54 2012 From: pathrm35 <@t> comcast.net (Ron) Date: Wed Aug 15 11:39:26 2012 Subject: [Histonet] RE: Two identifiers... Message-ID: We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, >use, disclosure or distribution is prohibited. If you are not the >intended recipient, please contact the sender by reply >e-mail and destroy all copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Aug 15 11:54:17 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Aug 15 11:54:59 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: <00ef01cd7b06$ae594a80$0b0bdf80$@pathview.com> Our client labs have always used the barcode and the case id as the identifiers and we've never heard of a problem. This allows us to print special instructions on the cassette, which everyone finds helpful later in the workflow. PS: it also allows us to print the case id in a bigger font as well and that in itself may be the biggest 'bang for the buck'. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Aug 15 11:59:00 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Aug 15 11:59:08 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: Can a bar code be counted as an identifier? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Wed Aug 15 12:04:13 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Aug 15 12:04:28 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <00ef01cd7b06$ae594a80$0b0bdf80$@pathview.com> References: <00ef01cd7b06$ae594a80$0b0bdf80$@pathview.com> Message-ID: Good, because that's what I've been doing for several years! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 15, 2012 12:54 PM To: 'Ron'; pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... Our client labs have always used the barcode and the case id as the identifiers and we've never heard of a problem. This allows us to print special instructions on the cassette, which everyone finds helpful later in the workflow. PS: it also allows us to print the case id in a bigger font as well and that in itself may be the biggest 'bang for the buck'. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From PAMarcum <@t> uams.edu Wed Aug 15 12:19:00 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 15 12:19:34 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu> That I don't know as we have barcodes that the information is transferred from on the cassettes. It contains all of the patient info plus block and date information. Pam -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, August 15, 2012 11:59 AM To: 'Ron'; Marcum, Pamela A; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... Can a bar code be counted as an identifier? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PAMarcum <@t> uams.edu Wed Aug 15 12:23:40 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 15 12:26:13 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF4174054559@EXCHANGESB> References: <64DB27005E2FD3439E88502D7A5C9121E98DD899E9@CORTEZ.ucdenver.pvt>, <14E2C6176416974295479C64A11CB9AE0162D07DACEA@SBS2K8.premierlab.local> <0B8979A204680A42B93A52B486088CD92CCB563DAB@CUAEXH1.GCU-MD.local> <50DA0C6B72976B4AB3A0FCA04CC73DBF4174054559@EXCHANGESB> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3BBD@Mail2Node1.ad.uams.edu> We use the Thermo Color Frost charged slides. The clip corners don't work well so be careful with those as it may be a problem. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, August 15, 2012 11:15 AM To: 'Walter Benton'; Elizabeth Chlipala; 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems We are getting the PrintMate and SlideMate for our lab very soon. What brand of slides are you using that works best? Are they white slides or colored? Thank you in advance for your input. Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, August 15, 2012 12:03 PM To: Elizabeth Chlipala; 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I too have the same systems that are being referenced. The Printmate did have a ton of problems with the original print heads, however they are doing a sweep to replace all of them according to my rep. We no longer experience the broken pins or other associated issues with the Print Mate. As for the slide mate, we had similar issues with that as well and found that after it was serviced a second time that we needed upgraded software/firmware on the instrument itself. Another issue that goes untouched by many users is the advanced user menu, which controls the pressure in which the thermal head presses on the slides and a variety of other attributes. Most reps are not aware of these features and thus are unable to help resolve your issues. Once we found out about these settings our slidemates work great. In addition to the advance settings on the slide mate it is important to wipe the thermal print head with an alcohol wipe daily to keep it clean performing optimally. Another issue that I have witnessed is that some of the paint on some slides do not work as well as others. I recently tested a slide with paint specifically formulated for the slide mate and the print was the best I have ever seen. Overall the units work well provided they are maintained and you have a knowledgeable support person to help you test out some of the advance menu features. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala [liz@premierlab.com] Sent: Wednesday, August 15, 2012 11:28 AM To: 'Bell, Pat'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems We had the slide mate and we do not use it any more, its worthless. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 15, 2012 9:26 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told to send it in to get it repaired but I wonder if it is worth the time and money if you are getting it back in basically the same condition! I know the Leica is pretty good but I am in research and can't spend that kind of money. I wish there was a system that was good and not extremely expensive. Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From joelleweaver <@t> hotmail.com Wed Aug 15 12:31:42 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 15 12:31:48 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu> References: , , <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu> Message-ID: I have worked many places that used barcodes/QR codes as an identifier. I have never heard of this being a problem, and actually seemed to be a well regarded method for the second identifier. Names can be the same, numbers can get transposed....I also thought this preferable since the patient name is not associated with the accession number wherever the slide may travel. The bar code should be "machine readable" with most readers since the symbologies are fairly standardized now. So you can always verify the information, and if you use the coding for work tracking, this is pretty standard I believe, and far more accurate than most folks with numbers and names ( though still not perfect). Joelle Weaver MAOM, HTL (ASCP) QIHC From: PAMarcum@uams.edu To: Joyce.Weems@emoryhealthcare.org; pathrm35@comcast.net; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Date: Wed, 15 Aug 2012 17:19:00 +0000 Subject: RE: [Histonet] RE: Two identifiers... CC: That I don't know as we have barcodes that the information is transferred from on the cassettes. It contains all of the patient info plus block and date information. Pam -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, August 15, 2012 11:59 AM To: 'Ron'; Marcum, Pamela A; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... Can a bar code be counted as an identifier? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Aug 15 12:33:42 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Aug 15 12:34:33 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu> Message-ID: <011601cd7b0c$34e43f10$9eacbd30$@pathview.com> While on the subject of identifiers on slide labels, I was wondering if anyone had ever run into a concern regarding patient name on slides that are sent out for further stains, consultation, etc.? I've run into at least one lab that was concerned that it was a HIPPA/patient confidentiality violation. Has anyone else run into this issue or given this topic some consideration? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Wednesday, August 15, 2012 1:19 PM To: 'Weems, Joyce K.'; 'Ron'; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... That I don't know as we have barcodes that the information is transferred from on the cassettes. It contains all of the patient info plus block and date information. Pam -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, August 15, 2012 11:59 AM To: 'Ron'; Marcum, Pamela A; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... Can a bar code be counted as an identifier? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From oneilb <@t> wvuhealthcare.com Wed Aug 15 12:39:05 2012 From: oneilb <@t> wvuhealthcare.com (Oneil, Beth Ann) Date: Wed Aug 15 12:39:14 2012 Subject: [Histonet] RE: Slide Printing Systems Message-ID: <3CEB8EBCF9C7A648B9694B5696462A7102A769@NT-EXMB2.wvuh.wvuhs.com> In response to the following question from earlier: "We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice" Our laboratory has been using the Slide Mate for almost a year and it was a very rough time for everyone because it didn't work as smoothly as we had seen at another institution. We had a lot of issues with the print quality of our slides as well and we were always being given some excuse. After months of complaining, our sales rep finally gave us a tip-sheet for maintenance of the printers and that has really helped a lot. We have also seen issues with the printing of the cassettes. We have gone through at least 3 new print heads in less than a year. We keep asking for a spare print head so that we can install it ourselves and eliminate any downtime, but we have yet to receive one. We also have issues with the barcode scanners. Some work better than others. It was a very frustrating process and my only recommendation is to keep complaining to your sales rep and hound them constantly until you're satisfied. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals 304-293-7629 (office) 304-293-6014 (lab) From joewalker <@t> rrmc.org Wed Aug 15 12:47:06 2012 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Wed Aug 15 12:47:12 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <011601cd7b0c$34e43f10$9eacbd30$@pathview.com> References: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu> <011601cd7b0c$34e43f10$9eacbd30$@pathview.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC730C5B@RRMBX03.rrmc.local> First, the two identifier rule applies to specimens arriving into the laboratory. Once you assign a unique lab identifier eg. an accession number, that is all that is required within the laboratory. This is from the CAP regulations. Second, ask the lab with the issue, exactly what protected health information is on the slide label? A person's name even DOB is NOT considered protected health information even under the latest HIPAA revisions. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 15, 2012 1:34 PM To: 'Marcum, Pamela A'; 'Weems, Joyce K.'; 'Ron'; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... While on the subject of identifiers on slide labels, I was wondering if anyone had ever run into a concern regarding patient name on slides that are sent out for further stains, consultation, etc.? I've run into at least one lab that was concerned that it was a HIPPA/patient confidentiality violation. Has anyone else run into this issue or given this topic some consideration? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Wednesday, August 15, 2012 1:19 PM To: 'Weems, Joyce K.'; 'Ron'; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... That I don't know as we have barcodes that the information is transferred from on the cassettes. It contains all of the patient info plus block and date information. Pam -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, August 15, 2012 11:59 AM To: 'Ron'; Marcum, Pamela A; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... Can a bar code be counted as an identifier? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From allyse124 <@t> gmail.com Wed Aug 15 13:52:42 2012 From: allyse124 <@t> gmail.com (Allyse Mazzarelli) Date: Wed Aug 15 13:52:46 2012 Subject: [Histonet] Specimen Blocks too Soft to Cut Message-ID: This question pertains to anyone that uses the Technovit 7100 kit. My lab has seen problems with humidity recently? (lab is too big to use a dehumidifier). I embed with enough hardener and mix my embedding solution well, but when I take my samples out of the mold they are still soft. I have tried to store them in an air tight container in dessicant beads over night, but that only partially works. Any other suggestions? Has anyone else that uses this kit ran into a similar problem? Thanks in advance to anyone that can send me some advice!! Sincerely, Allyse Mazzarelli NEUROTECH USA INC. From nto <@t> stowers.org Wed Aug 15 15:15:31 2012 From: nto <@t> stowers.org (Thomas, Nancy) Date: Wed Aug 15 15:15:38 2012 Subject: [Histonet] Specimen Blocks too Soft to Cut In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9B024558B@EXCHMB-02.stowers-institute.org> Allyse, I used to have those problems, too. I tried JB-4 and T-7100 and L.R. White. Sometimes I had great results, sometimes not. The results were never consistent. Then one glorious day, someone from another lab suggested I try Immuno-bed. It is amazing. I have not had those issues ever again. It sets up so quickly. If I make and use the embedding solution in the morning, I can trim and glue my samples to the chuck later that same day. Unbelievable! I get it from Poly-Sciences. Please try it! Nancy Thomas Stowers Institute for Medical Research Kansas City, MO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allyse Mazzarelli Sent: Wednesday, August 15, 2012 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Blocks too Soft to Cut This question pertains to anyone that uses the Technovit 7100 kit. My lab has seen problems with humidity recently... (lab is too big to use a dehumidifier). I embed with enough hardener and mix my embedding solution well, but when I take my samples out of the mold they are still soft. I have tried to store them in an air tight container in dessicant beads over night, but that only partially works. Any other suggestions? Has anyone else that uses this kit ran into a similar problem? Thanks in advance to anyone that can send me some advice!! Sincerely, Allyse Mazzarelli NEUROTECH USA INC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Aug 15 16:26:02 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 15 16:26:07 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <011601cd7b0c$34e43f10$9eacbd30$@pathview.com> References: , <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu>, <011601cd7b0c$34e43f10$9eacbd30$@pathview.com> Message-ID: Yes, some of the labs where I have worked have been concerned and overly cautious I guess (since I saw the correction that apparently it is not technically a HIPPA issue to have the name etc on the slide). But anyhow, as I understood it their decisions was based on the idea that they they felt that names were not truly unique. So they liked the idea that all the identifiers would be assigned as numbers and coding .They also wanted the 2 identifiers required at receipt to be an "across the board procedure" for all lab specimens that came into any lab area. Using these methods was more consistent with other areas and the procedure for rejection due to specimen identity defects and other non-conformity was spelled out clearly for all types of lab specimens and documented the same way. As I recall,the JC patient safety goals , played into their decisions at the time, and also a desire for more consistency and standardization of processes. Personally never liked using names, numbers are ok since you have to order and sort somehow, but I like that you have the potential to get added benefits for data and workflow tracking with barcoding and other imprinting. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mike@pathview.com > To: PAMarcum@uams.edu; Joyce.Weems@emoryhealthcare.org; pathrm35@comcast.net; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu > Date: Wed, 15 Aug 2012 13:33:42 -0400 > Subject: RE: [Histonet] RE: Two identifiers... > CC: > > While on the subject of identifiers on slide labels, I was wondering if anyone had ever run into a concern regarding patient name on slides that are sent out for further stains, consultation, etc.? > > I've run into at least one lab that was concerned that it was a HIPPA/patient confidentiality violation. > > Has anyone else run into this issue or given this topic some consideration? > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A > Sent: Wednesday, August 15, 2012 1:19 PM > To: 'Weems, Joyce K.'; 'Ron'; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Two identifiers... > > That I don't know as we have barcodes that the information is transferred from on the cassettes. It contains all of the patient info plus block and date information. Pam > > -----Original Message----- > From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] > Sent: Wednesday, August 15, 2012 11:59 AM > To: 'Ron'; Marcum, Pamela A; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Two identifiers... > > Can a bar code be counted as an identifier? > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron > Sent: Wednesday, August 15, 2012 12:39 PM > To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: Two identifiers... > > We do the same as Pam with the patient name and case number > > Sent from my Verizon Wireless 4G LTE smartphone > > > > "Marcum, Pamela A" wrote: > > >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > > > >Pam Marcum > >UAMS > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom > >McNemar > >Sent: Wednesday, August 15, 2012 10:03 AM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Two identifiers... > > > >Hello all, > > > >For those of you using cassette and slide labelers.... > >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > > > >Tom McNemar, HT(ASCP) > >Histology Co-ordinator > >Licking Memorial Health Systems > >(740) 348-4163 > >(740) 348-4166 > >tmcnemar@lmhealth.org > >www.LMHealth.org >tion%20Data\Microsoft\Signatures\www.LMHealth.org> > > > >________________________________ > >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice: This e-mail message, including any attachments, > >is for the sole use of the intended recipient(s) and may contain > >confidential and privileged information. Any unauthorized review, use, > >disclosure or distribution is prohibited. If you are not the intended > >recipient, please contact the sender by reply e-mail and destroy all > >copies of the original message. > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Aug 15 16:49:33 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 15 16:49:40 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC730C5B@RRMBX03.rrmc.local> References: , , <41D3A1AF6FEF0643BDC89E0516A6EA3265FE3B90@Mail2Node1.ad.uams.edu>, <011601cd7b0c$34e43f10$9eacbd30$@pathview.com>, <3C2378778400AD448ADA6FD6BDB7CCCC730C5B@RRMBX03.rrmc.local> Message-ID: The de-indentification rules in HIPPA [45 CFR 164.514] stipulate alot of personal information as PHI ( all for electronic media/transmission/retrieval/access etc) such as "All elements .... directly related to an individual, including names, birth date, admission date,discharge date, date of death, Medical record numbers......" . I know HIPPA rules go on to more explicit details for encoding and decoding personal information, and specifically stipulate the encryption etc- subjects that I should probably leave to those people who work with this directly. Anyhow, I have not taken a recent look at the HITECH act so not sure how it plays in to respond here, but I recall that it elaborates further. I don't have a checklist handy, but I would think that if you use an LIS with a patient data base, electronically send reports, fax reports/requisitions etc, this would mean you have to comply. I guess overall, most places I have worked at have interpreted the protection of PHI strictly to mean all information that relates to the patient identity, and have kept it as private as possible and restricted it unless you have that all important "need to know". Joelle Weaver MAOM, HTL (ASCP) QIHC From: joewalker@rrmc.org To: mike@pathview.com; PAMarcum@uams.edu; Joyce.Weems@emoryhealthcare.org; pathrm35@comcast.net; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Date: Wed, 15 Aug 2012 17:47:06 +0000 Subject: RE: [Histonet] RE: Two identifiers... CC: First, the two identifier rule applies to specimens arriving into the laboratory. Once you assign a unique lab identifier eg. an accession number, that is all that is required within the laboratory. This is from the CAP regulations. Second, ask the lab with the issue, exactly what protected health information is on the slide label? A person's name even DOB is NOT considered protected health information even under the latest HIPAA revisions. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 15, 2012 1:34 PM To: 'Marcum, Pamela A'; 'Weems, Joyce K.'; 'Ron'; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... While on the subject of identifiers on slide labels, I was wondering if anyone had ever run into a concern regarding patient name on slides that are sent out for further stains, consultation, etc.? I've run into at least one lab that was concerned that it was a HIPPA/patient confidentiality violation. Has anyone else run into this issue or given this topic some consideration? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Wednesday, August 15, 2012 1:19 PM To: 'Weems, Joyce K.'; 'Ron'; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... That I don't know as we have barcodes that the information is transferred from on the cassettes. It contains all of the patient info plus block and date information. Pam -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, August 15, 2012 11:59 AM To: 'Ron'; Marcum, Pamela A; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... Can a bar code be counted as an identifier? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cebass <@t> buffalo.edu Wed Aug 15 17:07:46 2012 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Wed Aug 15 17:07:51 2012 Subject: [Histonet] protocol for EGFP IHC in frozen rat brains Message-ID: Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline From rsrichmond <@t> gmail.com Wed Aug 15 20:11:12 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Aug 15 20:11:15 2012 Subject: [Histonet] Re: Two identifiers... Message-ID: If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN From joelleweaver <@t> hotmail.com Thu Aug 16 00:24:56 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Aug 16 00:25:02 2012 Subject: [Histonet] Re: Two identifiers... In-Reply-To: References: Message-ID: Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill? I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone. I realize barcodes are not helpful to people doing handling unfortunately, but they do help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious, and allow tracking for workflow- but many people still prefer human-manual labeling methods. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 15 Aug 2012 21:11:12 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Two identifiers... > > If a pathologist end-user may make an observation here: > > When I pick up a slide and look to match it with the paperwork in > front of me, it's a great help if the patient's name is on the slide, > since names are always easier to read than numbers. Having the > patient's name on the slide means I'm less likely to mix up two cases. > I can't do that with a bar code or a second essentially meaningless > number. > > This may however be a situation where good patient care must take a > back seat to good management practice and good regulator compliance. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Aug 16 04:04:17 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Aug 16 04:10:15 2012 Subject: [Histonet] Re: Two identifiers... In-Reply-To: References: Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com> Bob, I absolutely concur with your thoughts, but in the LIS we offer, the moment you scan the slide, the 'paperwork' displays on the screen automatically, effectively negating the need to match paperwork with the slide. We also always recommend BIG monitors or even dual monitors to make it easier to see. Imagine the paperwork on one monitor and a 'work' screen on the other monitor. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, August 15, 2012 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two identifiers... If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Judith_Pardue <@t> memorial.org Thu Aug 16 05:49:50 2012 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Thu Aug 16 05:50:27 2012 Subject: [Histonet] Negative Control Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9BCD@chimsx03.CHI.catholichealth.net> At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections. Judith Pardue Histology Supervisor Memorial Hospital Chattanooga, Tn. Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From hymclab.hymclab <@t> ministryhealth.org Thu Aug 16 07:51:41 2012 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Aug 16 07:52:04 2012 Subject: [Histonet] Special Stain documentation In-Reply-To: References: <20120813143522.295dc6182df7e5cbb4f32bc101c30dcc.57d85340c8.wbe@email15.secureserver.net> Message-ID: For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials. The lead tech also initials. For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains. The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, August 13, 2012 5:06 PM To: 'Laurie@blufrogpath.com'; Histonet post Subject: RE: [Histonet] Special Stain documentation Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Judith_Pardue <@t> memorial.org Thu Aug 16 08:17:20 2012 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Thu Aug 16 08:26:10 2012 Subject: [Histonet] Peloris Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9C2F@chimsx03.CHI.catholichealth.net> Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and plugged into an emergency outlet. This is the third time this has happened and we have had to have a service tech from Leica come out. Judith Pardue Histology Supervisor Memorial Hospital Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Caroline.Pratt <@t> uphs.upenn.edu Thu Aug 16 08:46:54 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Aug 16 08:47:07 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From TMcNemar <@t> lmhealth.org Thu Aug 16 08:50:45 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Aug 16 08:50:57 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From kmerriam2003 <@t> yahoo.com Thu Aug 16 09:01:11 2012 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Aug 16 09:01:20 2012 Subject: [Histonet] marker specific for mouse fibroblasts Message-ID: <1345125671.55062.YahooMailNeo@web130105.mail.mud.yahoo.com> Hello All! I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Caroline.Pratt <@t> uphs.upenn.edu Thu Aug 16 09:02:07 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Aug 16 09:02:18 2012 Subject: [Histonet] RE: Two identifiers... In-Reply-To: References: Message-ID: I am so sorry to cause confusion, I am referring to specimen bottles, my apologies. -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, August 16, 2012 9:51 AM To: Pratt, Caroline; Ron; pamarcum@uams.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From liz <@t> premierlab.com Thu Aug 16 09:51:32 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Aug 16 09:51:37 2012 Subject: [Histonet] marker specific for mouse fibroblasts In-Reply-To: <1345125671.55062.YahooMailNeo@web130105.mail.mud.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DAD12@SBS2K8.premierlab.local> Kim There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse. I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse. Acris has a lot of information on their website. In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, August 16, 2012 8:01 AM To: Histonet Subject: [Histonet] marker specific for mouse fibroblasts Hello All! I am looking for a marker that is highly specific for mouse fibroblasts. I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Fri Aug 17 06:59:01 2012 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Aug 17 06:59:07 2012 Subject: [Histonet] Re: Eosin Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40E50@isexstore03> I just wanted to thank everyone who has responded to my message. All of the input is greatly appreciated. This is a great site to be utilized in our quest for Histology perfection. Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ________________________________ From: Hannen, Valerie Sent: Thursday, August 09, 2012 11:23 AM To: histonet Subject: Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com * ======================== ======= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ======================== ======= From pkromund <@t> gundluth.org Fri Aug 17 10:24:41 2012 From: pkromund <@t> gundluth.org (Romundstad, Pamela K) Date: Fri Aug 17 10:26:50 2012 Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 In-Reply-To: References: Message-ID: Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: "Bass, Caroline" Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline ------------------------------ Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 3 Date: Thu, 16 Aug 2012 05:24:56 +0000 From: joelle weaver Subject: RE: [Histonet] Re: Two identifiers... To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill? I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone. I realize barcodes are not helpful to people doing handling unfortunately, but they do help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious, and allow tracking for workflow- but many people still prefer human-manual labeling methods. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 15 Aug 2012 21:11:12 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Two identifiers... > > If a pathologist end-user may make an observation here: > > When I pick up a slide and look to match it with the paperwork in > front of me, it's a great help if the patient's name is on the slide, > since names are always easier to read than numbers. Having the > patient's name on the slide means I'm less likely to mix up two cases. > I can't do that with a bar code or a second essentially meaningless > number. > > This may however be a situation where good patient care must take a > back seat to good management practice and good regulator compliance. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 16 Aug 2012 05:04:17 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: Two identifiers... To: "'Bob Richmond'" , Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Bob, I absolutely concur with your thoughts, but in the LIS we offer, the moment you scan the slide, the 'paperwork' displays on the screen automatically, effectively negating the need to match paperwork with the slide. We also always recommend BIG monitors or even dual monitors to make it easier to see. Imagine the paperwork on one monitor and a 'work' screen on the other monitor. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, August 15, 2012 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two identifiers... If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 16 Aug 2012 04:49:50 -0600 From: "Pardue, Judith" Subject: [Histonet] Negative Control To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9BCD@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections. Judith Pardue Histology Supervisor Memorial Hospital Chattanooga, Tn. Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 6 Date: Thu, 16 Aug 2012 07:51:41 -0500 From: hymclab Subject: RE: [Histonet] Special Stain documentation To: "'Weems, Joyce K.'" , "'laurie@blufrogpath.com'" , Histonet post Message-ID: Content-Type: text/plain; charset="us-ascii" For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials. The lead tech also initials. For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains. The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, August 13, 2012 5:06 PM To: 'Laurie@blufrogpath.com'; Histonet post Subject: RE: [Histonet] Special Stain documentation Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ Message: 7 Date: Thu, 16 Aug 2012 07:17:20 -0600 From: "Pardue, Judith" Subject: [Histonet] Peloris To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9C2F@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and plugged into an emergency outlet. This is the third time this has happened and we have had to have a service tech from Leica come out. Judith Pardue Histology Supervisor Memorial Hospital Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 8 Date: Thu, 16 Aug 2012 09:46:54 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Ron" , , , Message-ID: Content-Type: text/plain; charset="utf-8" It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 9 Date: Thu, 16 Aug 2012 09:50:45 -0400 From: Tom McNemar Subject: RE: [Histonet] RE: Two identifiers... To: "'Pratt, Caroline'" , Ron , "pamarcum@uams.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 10 Date: Thu, 16 Aug 2012 07:01:11 -0700 (PDT) From: Kim Merriam Subject: [Histonet] marker specific for mouse fibroblasts To: Histonet Message-ID: <1345125671.55062.YahooMailNeo@web130105.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello All! I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 11 Date: Thu, 16 Aug 2012 10:02:07 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Tom McNemar" , "Ron" , , Message-ID: Content-Type: text/plain; charset="utf-8" I am so sorry to cause confusion, I am referring to specimen bottles, my apologies. -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, August 16, 2012 9:51 AM To: Pratt, Caroline; Ron; pamarcum@uams.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 12 Date: Thu, 16 Aug 2012 08:51:32 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] marker specific for mouse fibroblasts To: 'Kim Merriam' , Histonet Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DAD12@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Kim There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse. I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse. Acris has a lot of information on their website. In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, August 16, 2012 8:01 AM To: Histonet Subject: [Histonet] marker specific for mouse fibroblasts Hello All! I am looking for a marker that is highly specific for mouse fibroblasts. I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 20 ***************************************** From Debbie.Lake <@t> mgh.net Fri Aug 17 10:29:16 2012 From: Debbie.Lake <@t> mgh.net (Lake,Debbie) Date: Fri Aug 17 10:29:20 2012 Subject: [Histonet] disposal of formalin Message-ID: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. From vperez <@t> pathreflab.com Fri Aug 17 10:25:25 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Fri Aug 17 10:32:11 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: We neutralize it and then dump down the drain with tons of running water. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Aug 17 10:34:28 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Aug 17 10:34:35 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: Same here - with Neutralex. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, August 17, 2012 11:25 AM To: Lake,Debbie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disposal of formalin We neutralize it and then dump down the drain with tons of running water. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From JThawley <@t> ShoreMemorial.org Fri Aug 17 10:35:40 2012 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Fri Aug 17 10:36:00 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: Message-ID: We do the same. Jennifer Thawley HT, ASCP Histology Supervisor Shore Medical Center (609) 653-3940 Vanessa Perez To Sent by: "Lake,Debbie" histonet-bounces@ , lists.utsouthwest "histonet@lists.utsouthwestern.edu" ern.edu cc 08/17/2012 11:25 Subject AM [Histonet] RE: disposal of formalin We neutralize it and then dump down the drain with tons of running water. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Aug 17 10:38:17 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Aug 17 10:38:21 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1F9A36C1@evcspmbx2.ads.northwestern.edu> Our department of Research Safety picks it up (5 gal) container. They are responsible for disposal. I believe it goes offsite. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 17 11:11:44 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 17 11:11:47 2012 Subject: [Histonet] disposal of formalin In-Reply-To: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: <1345219904.88155.YahooMailNeo@web121405.mail.ne1.yahoo.com> After testing ALL available "neutralizers" in the market I was able to detect the presence of "aldehyde" (formalin) in the "neutralized" product. In consequence I decided that the best option, albeit not the cheapest, was to contract a specialized company to take of the disposal. Mind that even when?you contract somebody to do your disposal, your lab is responsible for any disposal problems. Your lab is responsible for the formalin you use "from cradle to tomb" Ren? J.? ________________________________ From: "Lake,Debbie" To: histonet@lists.utsouthwestern.edu Sent: Friday, August 17, 2012 11:29 AM Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility?? Neutralization, disposal off site, etc.? Thank you. Debra Lake? MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN? 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Fri Aug 17 11:31:41 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Aug 17 11:31:58 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: We hire a licensed waste disposal company. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Aug 17 11:35:55 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Aug 17 11:36:53 2012 Subject: [Histonet] RE: negative controls Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A17C4@EVS1.archildrens.org> For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: "Bass, Caroline" Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline ------------------------------ Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 3 Date: Thu, 16 Aug 2012 05:24:56 +0000 From: joelle weaver Subject: RE: [Histonet] Re: Two identifiers... To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill? I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone. I realize barcodes are not helpful to people doing handling unfortunately, but they do help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious, and allow tracking for workflow- but many people still prefer human-manual labeling methods. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 15 Aug 2012 21:11:12 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Two identifiers... > > If a pathologist end-user may make an observation here: > > When I pick up a slide and look to match it with the paperwork in > front of me, it's a great help if the patient's name is on the slide, > since names are always easier to read than numbers. Having the > patient's name on the slide means I'm less likely to mix up two cases. > I can't do that with a bar code or a second essentially meaningless > number. > > This may however be a situation where good patient care must take a > back seat to good management practice and good regulator compliance. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 16 Aug 2012 05:04:17 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: Two identifiers... To: "'Bob Richmond'" , Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Bob, I absolutely concur with your thoughts, but in the LIS we offer, the moment you scan the slide, the 'paperwork' displays on the screen automatically, effectively negating the need to match paperwork with the slide. We also always recommend BIG monitors or even dual monitors to make it easier to see. Imagine the paperwork on one monitor and a 'work' screen on the other monitor. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, August 15, 2012 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two identifiers... If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 16 Aug 2012 04:49:50 -0600 From: "Pardue, Judith" Subject: [Histonet] Negative Control To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9BCD@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections. Judith Pardue Histology Supervisor Memorial Hospital Chattanooga, Tn. Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 6 Date: Thu, 16 Aug 2012 07:51:41 -0500 From: hymclab Subject: RE: [Histonet] Special Stain documentation To: "'Weems, Joyce K.'" , "'laurie@blufrogpath.com'" , Histonet post Message-ID: Content-Type: text/plain; charset="us-ascii" For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials. The lead tech also initials. For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains. The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, August 13, 2012 5:06 PM To: 'Laurie@blufrogpath.com'; Histonet post Subject: RE: [Histonet] Special Stain documentation Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ Message: 7 Date: Thu, 16 Aug 2012 07:17:20 -0600 From: "Pardue, Judith" Subject: [Histonet] Peloris To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9C2F@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and plugged into an emergency outlet. This is the third time this has happened and we have had to have a service tech from Leica come out. Judith Pardue Histology Supervisor Memorial Hospital Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 8 Date: Thu, 16 Aug 2012 09:46:54 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Ron" , , , Message-ID: Content-Type: text/plain; charset="utf-8" It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 9 Date: Thu, 16 Aug 2012 09:50:45 -0400 From: Tom McNemar Subject: RE: [Histonet] RE: Two identifiers... To: "'Pratt, Caroline'" , Ron , "pamarcum@uams.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 10 Date: Thu, 16 Aug 2012 07:01:11 -0700 (PDT) From: Kim Merriam Subject: [Histonet] marker specific for mouse fibroblasts To: Histonet Message-ID: <1345125671.55062.YahooMailNeo@web130105.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello All! I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 11 Date: Thu, 16 Aug 2012 10:02:07 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Tom McNemar" , "Ron" , , Message-ID: Content-Type: text/plain; charset="utf-8" I am so sorry to cause confusion, I am referring to specimen bottles, my apologies. -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, August 16, 2012 9:51 AM To: Pratt, Caroline; Ron; pamarcum@uams.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 12 Date: Thu, 16 Aug 2012 08:51:32 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] marker specific for mouse fibroblasts To: 'Kim Merriam' , Histonet Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DAD12@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Kim There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse. I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse. Acris has a lot of information on their website. In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, August 16, 2012 8:01 AM To: Histonet Subject: [Histonet] marker specific for mouse fibroblasts Hello All! I am looking for a marker that is highly specific for mouse fibroblasts. I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 20 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Joyce.Weems <@t> emoryhealthcare.org Fri Aug 17 11:44:13 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Aug 17 11:45:12 2012 Subject: [Histonet] RE: negative controls In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A17C4@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A17C4@EVS1.archildrens.org> Message-ID: We aren't. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, August 17, 2012 12:36 PM To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: "Bass, Caroline" Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline ------------------------------ Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 3 Date: Thu, 16 Aug 2012 05:24:56 +0000 From: joelle weaver Subject: RE: [Histonet] Re: Two identifiers... To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill? I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone. I realize barcodes are not helpful to people doing handling unfortunately, but they do help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious, and allow tracking for workflow- but many people still prefer human-manual labeling methods. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 15 Aug 2012 21:11:12 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Two identifiers... > > If a pathologist end-user may make an observation here: > > When I pick up a slide and look to match it with the paperwork in > front of me, it's a great help if the patient's name is on the slide, > since names are always easier to read than numbers. Having the > patient's name on the slide means I'm less likely to mix up two cases. > I can't do that with a bar code or a second essentially meaningless > number. > > This may however be a situation where good patient care must take a > back seat to good management practice and good regulator compliance. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 16 Aug 2012 05:04:17 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: Two identifiers... To: "'Bob Richmond'" , Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Bob, I absolutely concur with your thoughts, but in the LIS we offer, the moment you scan the slide, the 'paperwork' displays on the screen automatically, effectively negating the need to match paperwork with the slide. We also always recommend BIG monitors or even dual monitors to make it easier to see. Imagine the paperwork on one monitor and a 'work' screen on the other monitor. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, August 15, 2012 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two identifiers... If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 16 Aug 2012 04:49:50 -0600 From: "Pardue, Judith" Subject: [Histonet] Negative Control To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9BCD@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections. Judith Pardue Histology Supervisor Memorial Hospital Chattanooga, Tn. Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 6 Date: Thu, 16 Aug 2012 07:51:41 -0500 From: hymclab Subject: RE: [Histonet] Special Stain documentation To: "'Weems, Joyce K.'" , "'laurie@blufrogpath.com'" , Histonet post Message-ID: Content-Type: text/plain; charset="us-ascii" For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials. The lead tech also initials. For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains. The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, August 13, 2012 5:06 PM To: 'Laurie@blufrogpath.com'; Histonet post Subject: RE: [Histonet] Special Stain documentation Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ Message: 7 Date: Thu, 16 Aug 2012 07:17:20 -0600 From: "Pardue, Judith" Subject: [Histonet] Peloris To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9C2F@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and plugged into an emergency outlet. This is the third time this has happened and we have had to have a service tech from Leica come out. Judith Pardue Histology Supervisor Memorial Hospital Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 8 Date: Thu, 16 Aug 2012 09:46:54 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Ron" , , , Message-ID: Content-Type: text/plain; charset="utf-8" It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 9 Date: Thu, 16 Aug 2012 09:50:45 -0400 From: Tom McNemar Subject: RE: [Histonet] RE: Two identifiers... To: "'Pratt, Caroline'" , Ron , "pamarcum@uams.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 10 Date: Thu, 16 Aug 2012 07:01:11 -0700 (PDT) From: Kim Merriam Subject: [Histonet] marker specific for mouse fibroblasts To: Histonet Message-ID: <1345125671.55062.YahooMailNeo@web130105.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello All! I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 11 Date: Thu, 16 Aug 2012 10:02:07 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Tom McNemar" , "Ron" , , Message-ID: Content-Type: text/plain; charset="utf-8" I am so sorry to cause confusion, I am referring to specimen bottles, my apologies. -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, August 16, 2012 9:51 AM To: Pratt, Caroline; Ron; pamarcum@uams.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 12 Date: Thu, 16 Aug 2012 08:51:32 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] marker specific for mouse fibroblasts To: 'Kim Merriam' , Histonet Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DAD12@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Kim There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse. I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse. Acris has a lot of information on their website. In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, August 16, 2012 8:01 AM To: Histonet Subject: [Histonet] marker specific for mouse fibroblasts Hello All! I am looking for a marker that is highly specific for mouse fibroblasts. I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 20 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. 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Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Fri Aug 17 11:47:21 2012 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Fri Aug 17 11:47:42 2012 Subject: [Histonet] RE: negative controls In-Reply-To: <25A4DE08332B19499904459F00AAACB719BB4A17C4@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BB4A17C4@EVS1.archildrens.org> Message-ID: We eliminated them on August 1st, except in cases where they are specifically requested. So far we have run <10 negative slides. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, August 17, 2012 12:36 PM To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: "Bass, Caroline" Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline ------------------------------ Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 3 Date: Thu, 16 Aug 2012 05:24:56 +0000 From: joelle weaver Subject: RE: [Histonet] Re: Two identifiers... To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill? I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone. I realize barcodes are not helpful to people doing handling unfortunately, but they do help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious, and allow tracking for workflow- but many people still prefer human-manual labeling methods. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 15 Aug 2012 21:11:12 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Two identifiers... > > If a pathologist end-user may make an observation here: > > When I pick up a slide and look to match it with the paperwork in > front of me, it's a great help if the patient's name is on the slide, > since names are always easier to read than numbers. Having the > patient's name on the slide means I'm less likely to mix up two cases. > I can't do that with a bar code or a second essentially meaningless > number. > > This may however be a situation where good patient care must take a > back seat to good management practice and good regulator compliance. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 16 Aug 2012 05:04:17 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: Two identifiers... To: "'Bob Richmond'" , Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Bob, I absolutely concur with your thoughts, but in the LIS we offer, the moment you scan the slide, the 'paperwork' displays on the screen automatically, effectively negating the need to match paperwork with the slide. We also always recommend BIG monitors or even dual monitors to make it easier to see. Imagine the paperwork on one monitor and a 'work' screen on the other monitor. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, August 15, 2012 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Two identifiers... If a pathologist end-user may make an observation here: When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases. I can't do that with a bar code or a second essentially meaningless number. This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 16 Aug 2012 04:49:50 -0600 From: "Pardue, Judith" Subject: [Histonet] Negative Control To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9BCD@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections. Judith Pardue Histology Supervisor Memorial Hospital Chattanooga, Tn. Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 6 Date: Thu, 16 Aug 2012 07:51:41 -0500 From: hymclab Subject: RE: [Histonet] Special Stain documentation To: "'Weems, Joyce K.'" , "'laurie@blufrogpath.com'" , Histonet post Message-ID: Content-Type: text/plain; charset="us-ascii" For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials. The lead tech also initials. For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains. The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, August 13, 2012 5:06 PM To: 'Laurie@blufrogpath.com'; Histonet post Subject: RE: [Histonet] Special Stain documentation Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately". Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie@blufrogpath.com Sent: Monday, August 13, 2012 5:35 PM To: Histonet post Subject: [Histonet] Special Stain documentation How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ Message: 7 Date: Thu, 16 Aug 2012 07:17:20 -0600 From: "Pardue, Judith" Subject: [Histonet] Peloris To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89ABE9C2F@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and plugged into an emergency outlet. This is the third time this has happened and we have had to have a service tech from Leica come out. Judith Pardue Histology Supervisor Memorial Hospital Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 8 Date: Thu, 16 Aug 2012 09:46:54 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Ron" , , , Message-ID: Content-Type: text/plain; charset="utf-8" It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 9 Date: Thu, 16 Aug 2012 09:50:45 -0400 From: Tom McNemar Subject: RE: [Histonet] RE: Two identifiers... To: "'Pratt, Caroline'" , Ron , "pamarcum@uams.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 10 Date: Thu, 16 Aug 2012 07:01:11 -0700 (PDT) From: Kim Merriam Subject: [Histonet] marker specific for mouse fibroblasts To: Histonet Message-ID: <1345125671.55062.YahooMailNeo@web130105.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello All! I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ------------------------------ Message: 11 Date: Thu, 16 Aug 2012 10:02:07 -0400 From: "Pratt, Caroline" Subject: RE: [Histonet] RE: Two identifiers... To: "Tom McNemar" , "Ron" , , Message-ID: Content-Type: text/plain; charset="utf-8" I am so sorry to cause confusion, I am referring to specimen bottles, my apologies. -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, August 16, 2012 9:51 AM To: Pratt, Caroline; Ron; pamarcum@uams.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Pratt, Caroline [mailto:Caroline.Pratt@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamarcum@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamarcum@uams.edu; tmcnemar@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone "Marcum, Pamela A" wrote: >We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. > >Pam Marcum >UAMS > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Wednesday, August 15, 2012 10:03 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Two identifiers... > >Hello all, > >For those of you using cassette and slide labelers.... >Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.orgtion%20Data\Microsoft\Signatures\www.LMHealth.org> > >________________________________ >This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 12 Date: Thu, 16 Aug 2012 08:51:32 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] marker specific for mouse fibroblasts To: 'Kim Merriam' , Histonet Message-ID: <14E2C6176416974295479C64A11CB9AE0162D07DAD12@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Kim There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse. I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse. Acris has a lot of information on their website. In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, August 16, 2012 8:01 AM To: Histonet Subject: [Histonet] marker specific for mouse fibroblasts Hello All! I am looking for a marker that is highly specific for mouse fibroblasts. I have tried several markers, but none of them seem to be specific for ONLY fibroblasts. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 20 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Fri Aug 17 12:45:59 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Aug 17 12:46:02 2012 Subject: [Histonet] Re: disposal of formalin Message-ID: Proper disposal of formaldehyde is a topic that has been discussed many times on Histonet - Google: histosearch formalin disposal A great deal of misinformation circulates about this subject. I think basically it's what your local regulatory agencies - particularly the water department - want you to do. I'd particularly look at Dr. John Kiernan's note. Most of this discussion was around the turn of the century, and may be out of date. What I wrote on Histonet in 1999 still holds: We need to distinguish in our thinking among: *the environmental facts about what happens to formaldehyde * the rules imposed by regulatory agencies * the deceptions and concealments of commercial interests * the blind faith of most end users * and last, and definitely least, the fond musings of elderly pathologists. Bob Richmond Samurai Pathologist Maryville TN From Judith_Pardue <@t> memorial.org Fri Aug 17 13:08:19 2012 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Fri Aug 17 13:08:31 2012 Subject: [Histonet] Negative Controls Message-ID: <14B823F24E628E49BBFAD704E4BAB89AC39E4C@chimsx03.CHI.catholichealth.net> Does anyone know of any articles talking about not using negative controls when using a polymer based detection system. Judith Pardue Memorial Hospital Chattanooga, Tn. Judith_Pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From funderwood <@t> mcohio.org Fri Aug 17 14:48:49 2012 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Aug 17 14:49:14 2012 Subject: [Histonet] Microtome feedback Message-ID: <502E67E10200003400016254@mcohio.org> Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH From lblazek <@t> digestivespecialists.com Fri Aug 17 15:04:32 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 17 15:06:58 2012 Subject: [Histonet] RE: Slide printing systems In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39163A794BE4@IBMB7Exchange.digestivespecialists.com> I'm using the Primera Slide printer from Creative Waste Solutions. It is really working well for us. It's user friendly and so far we have had no problems. The really cool thing you can do is print a stripe of color across the top of the slide to designate a certain kind of procedure for that slide or designate it for a specific doctor. If you contact Rex at CWS http://cwsincorp.com/ he can tell you all about it. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pamela.Johnson <@t> STJUDE.ORG Fri Aug 17 15:09:18 2012 From: Pamela.Johnson <@t> STJUDE.ORG (Johnson, Pamela) Date: Fri Aug 17 15:09:24 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: <8F77913624F7524AACD2A92EAF3BFA544EEF950B42@11.stjude.org> May I ask what your neutralization protocol is? Pam Johnson, BS, HT (ASCP) Lab Manager Veterinary Pathology Core Lab St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office - 901-595-3355 -----Original Message----- From: Vanessa Perez [mailto:vperez@pathreflab.com] Sent: Friday, August 17, 2012 10:25 AM To: Lake,Debbie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disposal of formalin We neutralize it and then dump down the drain with tons of running water. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 17 15:21:42 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Aug 17 15:20:55 2012 Subject: [Histonet] RE: Slide printing systems Primera Message-ID: <761E2B5697F795489C8710BCC72141FFEE0C@ex07.net.ucsf.edu> Their website is here: http://www.primerahealthcare.com/signature-slide-printer.html Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, August 17, 2012 1:05 PM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide printing systems I'm using the Primera Slide printer from Creative Waste Solutions. It is really working well for us. It's user friendly and so far we have had no problems. The really cool thing you can do is print a stripe of color across the top of the slide to designate a certain kind of procedure for that slide or designate it for a specific doctor. If you contact Rex at CWS http://cwsincorp.com/ he can tell you all about it. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Wednesday, August 15, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printing systems Good Morning, We currently are using Thermo-Fisher print-mate and slide-mate. I am not very happy with the printing of the slides. Some days I feel like spend most of my time re-writing and labeling printed slides. Then when the units are sent out for repair and it arrives back it is not a whole lot better. Before we get our new LIS system and our Docs will be scanning the bar code(that sometimes do not print) I need to find a better system. I will not be able to deal with how often my name will be called out when the scanning of the slides fail. I would like any input on other systems pros and cons that are currently being used in histology labs. I know no system will be perfect but something has to be better. Thanks, Janice Janice A. Mitchell, BS, HT(ASCP) Histology Lead Tech Children's Hospital of Philadelphia Anatomic Pathology and Laboratory Medicine 324 S. 34th Street Philadelphia, Pa 19104-4399 215-590-1738(lab) 267-426-7754(office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Fri Aug 17 15:19:51 2012 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Fri Aug 17 15:22:57 2012 Subject: [Histonet] Microtome feedback In-Reply-To: <502E67E10200003400016254@mcohio.org> References: <502E67E10200003400016254@mcohio.org> Message-ID: Leica Sent from the iPhone of Kim Tournear ?? On Aug 17, 2012, at 2:48 PM, "Fred Underwood" wrote: > Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. > > Thanks, > Fred Underwood > Montgomery County Coroner's Office > Dayton, OH > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PAMarcum <@t> uams.edu Fri Aug 17 15:24:45 2012 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Aug 17 15:24:59 2012 Subject: [Histonet] Microtome feedback In-Reply-To: <502E67E10200003400016254@mcohio.org> References: <502E67E10200003400016254@mcohio.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3265FE4014@Mail2Node1.ad.uams.edu> The RM2255 is a workhorse. We have three of those and 3 RM2155s as an older model. The 2255 is the best. I had used the 2255 in the past to cut plastic with a tungsten carbide knife and it held up better than anything else available at the time. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, August 17, 2012 2:49 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From vperez <@t> pathreflab.com Fri Aug 17 15:18:21 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Fri Aug 17 15:25:05 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: <8F77913624F7524AACD2A92EAF3BFA544EEF950B42@11.stjude.org> References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> <8F77913624F7524AACD2A92EAF3BFA544EEF950B42@11.stjude.org> Message-ID: We use formalex green. http://americanbiosafety.com/PDF/ABS_FXG_Instructions.pdf is a link to the instructions for it we also keep a disposal log every time we dump the neutralized formalin down the drain.. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: Johnson, Pamela [mailto:Pamela.Johnson@STJUDE.ORG] Sent: Friday, August 17, 2012 3:09 PM To: Vanessa Perez; Lake,Debbie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disposal of formalin May I ask what your neutralization protocol is? Pam Johnson, BS, HT (ASCP) Lab Manager Veterinary Pathology Core Lab St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office - 901-595-3355 -----Original Message----- From: Vanessa Perez [mailto:vperez@pathreflab.com] Sent: Friday, August 17, 2012 10:25 AM To: Lake,Debbie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disposal of formalin We neutralize it and then dump down the drain with tons of running water. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer From mwstarbu <@t> mdanderson.org Fri Aug 17 15:36:35 2012 From: mwstarbu <@t> mdanderson.org (Starbuck,Michael W) Date: Fri Aug 17 15:36:34 2012 Subject: [Histonet] Microtome feedback In-Reply-To: <502E67E10200003400016254@mcohio.org> References: <502E67E10200003400016254@mcohio.org> Message-ID: <256363280A982F489DA7D9FDFD47DD8B3F05D9E3EE@DCPWVMBXC0VS3.mdanderson.edu> Leica RM2255 for plastic and paraffin - no problems -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, August 17, 2012 2:49 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH From BDeBrosse-Serra <@t> isisph.com Fri Aug 17 20:57:48 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Aug 17 21:00:12 2012 Subject: [Histonet] Microtome feedback In-Reply-To: <502E67E10200003400016254@mcohio.org> References: <502E67E10200003400016254@mcohio.org> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CEF3EF4@EXCHMB01.isis.local> Fred, The Leica RM2255 all the way! Go Dayton! Bea ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH From ibernard <@t> uab.edu Sat Aug 18 05:52:33 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sat Aug 18 05:52:47 2012 Subject: [Histonet] Microtome feedback In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F092CEF3EF4@EXCHMB01.isis.local> References: <502E67E10200003400016254@mcohio.org> <493CAA64F203E14E8823737B9EE0E25F092CEF3EF4@EXCHMB01.isis.local> Message-ID: What is great about the Leica RM2255? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Friday, August 17, 2012 8:58 PM To: Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback Fred, The Leica RM2255 all the way! Go Dayton! Bea ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Sat Aug 18 10:49:03 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Aug 18 10:49:26 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> <8F77913624F7524AACD2A92EAF3BFA544EEF950B42@11.stjude.org> Message-ID: We use Aldex, neutralize the formalin and dispose of the neutralized gel in the biohazard trash. The manufacturer says it can go in regular trash, but we choose to put it in biohazard trash. Michelle On Aug 17, 2012, at 4:18 PM, Vanessa Perez wrote: > We use formalex green. > http://americanbiosafety.com/PDF/ABS_FXG_Instructions.pdf > > is a link to the instructions for it > > we also keep a disposal log every time we dump the neutralized formalin down the drain.. > > Vanessa Perez Garcia > Histology Supervisor > Pathology Reference Lab > 210-892-3746 > 210-892-3732 > vperez@pathreflab.com > > > -----Original Message----- > From: Johnson, Pamela [mailto:Pamela.Johnson@STJUDE.ORG] > Sent: Friday, August 17, 2012 3:09 PM > To: Vanessa Perez; Lake,Debbie; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: disposal of formalin > > May I ask what your neutralization protocol is? > > Pam Johnson, BS, HT (ASCP) > Lab Manager > Veterinary Pathology Core Lab > St. Jude Children's Research Hospital > 262 Danny Thomas Place > Memphis, TN 38105-3678 > Office - 901-595-3355 > > -----Original Message----- > From: Vanessa Perez [mailto:vperez@pathreflab.com] > Sent: Friday, August 17, 2012 10:25 AM > To: Lake,Debbie; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: disposal of formalin > > We neutralize it and then dump down the drain with tons of running water. > > Vanessa Perez Garcia > Histology Supervisor > Pathology Reference Lab > 210-892-3746 > 210-892-3732 > vperez@pathreflab.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie > Sent: Friday, August 17, 2012 10:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] disposal of formalin > > Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. > > > > Debra Lake MT(ASCP) > > Manager Micro, Blood Bank, Pathology > > Marion General Hospital > > Marion, IN 46952 > > (765) 660-6521 > > Fax: (765-651-7330) > > > > > > > > > > > > If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kmendell <@t> goldbergmd.net Mon Aug 20 06:29:45 2012 From: kmendell <@t> goldbergmd.net (MaryK Mendell) Date: Mon Aug 20 06:31:53 2012 Subject: [Histonet] RE: disposal of formalin In-Reply-To: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> References: <8D6EDB72CECABB4A988C5BB087B61E4F020053D4@mghemail1.mgh.net> Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D33D@EXMBX01.mmeprod.cbeyond> We also use a company to pick it up along with xylene & alcohol And you are responsible from craddle to grave. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie [Debbie.Lake@mgh.net] Sent: Friday, August 17, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Mon Aug 20 08:55:39 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Aug 20 08:55:58 2012 Subject: [Histonet] Microtome feedback In-Reply-To: References: <502E67E10200003400016254@mcohio.org> <493CAA64F203E14E8823737B9EE0E25F092CEF3EF4@EXCHMB01.isis.local> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34B8C@EXCHMB01.isis.local> It is a high quality work horse. I've worked with newer and older microtomes of this very model and never had any problems. You can utilize the motorized function to minimize repetitive motion, but you can also use it manually if needed. What is there not to like? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: Ian R Bernard [mailto:ibernard@uab.edu] Sent: Saturday, August 18, 2012 3:53 AM To: Bea DeBrosse-Serra; Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback What is great about the Leica RM2255? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Friday, August 17, 2012 8:58 PM To: Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback Fred, The Leica RM2255 all the way! Go Dayton! Bea ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Mon Aug 20 09:27:35 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Aug 20 09:27:42 2012 Subject: [Histonet] Microtome feedback In-Reply-To: <502E67E10200003400016254@mcohio.org> References: <502E67E10200003400016254@mcohio.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D3F4@EXCHANGEMB1.hmc.hurleymc.com> We purchased the Leica RM2255 just over a year ago. It is the only one we have and is primarily used by one tech. In her absence, others use her machine. What is great about it, is that it is VERY easy to adapt to it. The controls are easy to figure out, cuts beautiful slides, and can be used either manually or completely automatic. Additionally, it has a "homing" option for the block holder.........pretty handy if you need to adjust the block holder often! In the future, I plan on replacing 2 more microtomes with this Leica microtome. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 3:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH From Carol.Fields <@t> Northside.com Mon Aug 20 11:20:37 2012 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Mon Aug 20 11:20:51 2012 Subject: [Histonet] Immuno Tech Message-ID: <731941C266951A47BEF11E5EFAAED9C911C4059C@nsmvexch01.northside.local> If anyone has an Immuno Job Description you are willing to share I would appreciate it. My email address is carol.fields@northside.com. Thanks to all. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Karen.Heckford <@t> DignityHealth.org Mon Aug 20 12:42:39 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Mon Aug 20 12:42:48 2012 Subject: [Histonet] Productivity Message-ID: Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From CThornton <@t> dahlchase.com Mon Aug 20 12:49:25 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Aug 20 12:49:29 2012 Subject: [Histonet] Ventana Symphony reagents Message-ID: We have some Symphony reagents and coverslips that we no longer need. If you are in the New England area, we could deliver them with our courier service. If you are outside of New England, if you pay the shipping we will send them. Please email me off list if you would like to know what we have and are interested in taking any/all of it. Have a great day everyone! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From techmana12 <@t> yahoo.com Mon Aug 20 13:12:50 2012 From: techmana12 <@t> yahoo.com (Dorothy Glass) Date: Mon Aug 20 13:12:52 2012 Subject: [Histonet] DAB precipitate on H. pylori Message-ID: <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? From vperez <@t> pathreflab.com Mon Aug 20 13:10:40 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Mon Aug 20 13:17:39 2012 Subject: [Histonet] DAB precipitate on H. pylori In-Reply-To: <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> References: <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> Message-ID: Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Monday, August 20, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB precipitate on H. pylori I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Mon Aug 20 13:37:55 2012 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Mon Aug 20 13:38:10 2012 Subject: [Histonet] Productivity In-Reply-To: References: Message-ID: Hi Karen, The best way to communicate with management is through data. If you have access to say the last 3 years of blocks and or slides cut and you compare it to accessions or cpt codes then you have a roadmap of where you have been in the past and where you are now. Do you have more or less employees and is this a temporary lull in numbers or has this dip occurred before. At least you will be able to formulate a more informed response to their request for cutting back hours. This is always a complicated issue and we have seen an increase in case complexity as well in our institution so our numbers of blocks vs. specimen count is skewed as well. Tell them you will cut hours but you will need all new equipment to continue to meet your deadlines! Ha!. Let us know how it turns out and good luck! Christie Gowan, HT (ASCP) UAB Hospital Histology Supervisor Surgical Pathology 205 934 4991 cgowan@uabmc.edu > From: Karen.Heckford@DignityHealth.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 20 Aug 2012 10:42:39 -0700 > Subject: [Histonet] Productivity > > Good Morning, > I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Aug 20 16:03:33 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 20 16:03:37 2012 Subject: [Histonet] Productivity In-Reply-To: References: Message-ID: <1345496613.86112.YahooMailNeo@web121401.mail.ne1.yahoo.com> Hi Karen: "Productivity" and its measures depends on who is going to use it. 1- For the pathologists as a whole they are interested in CASES they receive and sign either on a daily or monthly basis; 2- For management it is measured in how many cases the pathologists can sign and therefore the administration can bill/collect 3- as the Lead Tech you are interested is the work output of your staff specially how many blocks each can section either per hour or per shift or how many minutes it takes to complete a FS. Mind that the ONLY productivity rate defined by CAP is the time a FS can be diagnosed from the moment it is received at histology and that has to be 20 minutes or less. Nowadays almost every lab has an automated stainer so the number of slides has been "downgraded" as a productivity index, although it is not the same to?prepare 1 than 20 slides. The unit of time is also a subject of debate: some prefer the "hour" but from the whole lab the index most desired is the number of CASES a lab can complete every day. Under separate cover I am sending you some articles I wrote on the subject so you can select those productivity indices most fit your lab. Ren? J. ________________________________ From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, August 20, 2012 1:42 PM Subject: [Histonet] Productivity Good Morning, I know we have been over this before, how does your lab measure productivity?? Are you going by block and slide or by CPT code?? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value.? As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides.? I do everything from GI specimens to big breast cases.? They will tell me that our productivity is down and we need to cut hours.? I just do not know exactly how to drill it in to them that things are not always as they appear on paper.? I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennpeteelz <@t> verizon.net Mon Aug 20 16:38:44 2012 From: jennpeteelz <@t> verizon.net (Me) Date: Mon Aug 20 16:40:59 2012 Subject: [Histonet] Re: Histonet Digest, Vol 105, Issue 24 In-Reply-To: <0M9200GY4CKUQZA0@vms172081.mailsrvcs.net> References: <0M9200GY4CKUQZA0@vms172081.mailsrvcs.net> Message-ID: <1db21a96-209c-44f0-a517-da793748aa2c@blur> I have a question for all in Histoland... Concerning the CAP revision on the use of negative controls...I work in a NJ lab that does work for NY offices, CAP states we no longer need to run controls if using biotin free detection but does NY state board of health agree? Not running those extra slides is a huge money and time saver. We are using Leica Bond III and the polymer detection. Anyone have an answer or know where I can get the answer? Jen Elz Pathology Solutions Eatontown, NJ Sent from my Verizon Wireless Droid -----Original message----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, Aug 20, 2012 17:00:30 GMT+00:00 Subject: Histonet Digest, Vol 105, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: disposal of formalin (MaryK Mendell) 2. RE: Microtome feedback (Bea DeBrosse-Serra) 3. RE: Microtome feedback (Lynette Pavelich) 4. Immuno Tech (Carol Fields) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Aug 2012 07:29:45 -0400 From: MaryK Mendell Subject: [Histonet] RE: disposal of formalin To: "Lake,Debbie" , "histonet@lists.utsouthwestern.edu" Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D33D@EXMBX01.mmeprod.cbeyond> Content-Type: text/plain; charset="us-ascii" We also use a company to pick it up along with xylene & alcohol And you are responsible from craddle to grave. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie [Debbie.Lake@mgh.net] Sent: Friday, August 17, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 20 Aug 2012 06:55:39 -0700 From: Bea DeBrosse-Serra Subject: RE: [Histonet] Microtome feedback To: "'Ian R Bernard'" , Fred Underwood , Histonet Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34B8C@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" It is a high quality work horse. I've worked with newer and older microtomes of this very model and never had any problems. You can utilize the motorized function to minimize repetitive motion, but you can also use it manually if needed. What is there not to like? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: Ian R Bernard [mailto:ibernard@uab.edu] Sent: Saturday, August 18, 2012 3:53 AM To: Bea DeBrosse-Serra; Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback What is great about the Leica RM2255? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Friday, August 17, 2012 8:58 PM To: Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback Fred, The Leica RM2255 all the way! Go Dayton! Bea ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 20 Aug 2012 14:27:35 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Microtome feedback To: Fred Underwood , Histonet Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D3F4@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" We purchased the Leica RM2255 just over a year ago. It is the only one we have and is primarily used by one tech. In her absence, others use her machine. What is great about it, is that it is VERY easy to adapt to it. The controls are easy to figure out, cuts beautiful slides, and can be used either manually or completely automatic. Additionally, it has a "homing" option for the block holder.........pretty handy if you need to adjust the block holder often! In the future, I plan on replacing 2 more microtomes with this Leica microtome. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 3:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH ------------------------------ Message: 4 Date: Mon, 20 Aug 2012 12:20:37 -0400 From: "Carol Fields" Subject: [Histonet] Immuno Tech To: Cc: Message-ID: <731941C266951A47BEF11E5EFAAED9C911C4059C@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" If anyone has an Immuno Job Description you are willing to share I would appreciate it. My email address is carol.fields@northside.com. Thanks to all. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 24 ***************************************** From wilson6848 <@t> yahoo.com Mon Aug 20 17:23:06 2012 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Mon Aug 20 17:23:18 2012 Subject: [Histonet] New Position Message-ID: <1345501386.59218.YahooMailNeo@web125406.mail.ne1.yahoo.com> ? ? Hi, ??? I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY. Willing to relocate. Thanks, Wilson. From DSiena <@t> statlab.com Mon Aug 20 17:25:06 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Mon Aug 20 17:25:09 2012 Subject: [Histonet] Formalin and Operating Rooms Message-ID: Hi All, I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com From marktarango <@t> gmail.com Mon Aug 20 18:04:16 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 20 18:04:21 2012 Subject: [Histonet] Formalin and Operating Rooms In-Reply-To: References: Message-ID: Hi Debra, Formaldehyde was listed as "known to be a human carcinogen" in the 12th Report on Carcinogens (2011) put out by the Department of Health and Human Services. Here is a link http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf This is what is probably behind any recent changes to its use or availability. Johnson & Johnson just a few days ago announced that they're going to take formaldehyde out of baby shampoo! Yah! Mark On Mon, Aug 20, 2012 at 3:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs <@t> gmail.com Mon Aug 20 18:04:33 2012 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Mon Aug 20 18:04:37 2012 Subject: [Histonet] Formalin and Operating Rooms In-Reply-To: References: Message-ID: I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ewj <@t> pigsqq.org Mon Aug 20 18:20:06 2012 From: ewj <@t> pigsqq.org (E. Wayne Johnson) Date: Mon Aug 20 18:20:50 2012 Subject: [Histonet] annoying crystals on sections Message-ID: <5032C626.5040206@pigsqq.org> We are having problems with crystals precipitated on our slides which are H&E stains on tissues from pigs. Tissues are fixed in buffered formalin. We had trouble months ago with formalin pigment and we had resolved that by using ammonia in EtOH or picric acid in EtOH. Sometimes we receive fixed samples from the field that are not buffered but presently all of our tissues are fixed in neutral phosphate buffered formalin. We moved the Sakura autostainer to a different location under a fume hood on a different floor of the building to get the solvent odor out of our work area. Immediately we began to see a tremendous degradation in slide quality due to what we initially thought was formalin pigment. We have changed all of the solutions and all of the stains. We find that if we use Milli-q water instead of tap water for rinsing (done by hand in that case) we dont see the crystals, but the eosin staining quality is not acceptable after rinsing in the acidic (ph ~5) Milli-Q water. Our tap water is neutral to slightly alkaline and is very hard with calcium. We do all sorts of tissues for diagnosis of pig diseases. Sometimes the slides are quite acceptable but sometimes particularly when looking at small intestine, the crystals are very annoying. The crystals occur randomly on the slide except that there is a tendency for them to be centered on nuclei particularly in intestinal epithelium. The crystals are birefringent in polarized light but seem to be generally clear not dark like the formalin pigment we had seen before. Neither ammonia nor picric acid remove these, and now if we use alcoholic ammonia to treat the slides, the slides come out too blue. Our slides are cut at 4 to 5 microns. Brain has the least problem, small intestine seems worst. We have gone back and cut some blocks that previously stained beautifully with no pigment or precipitate problems and those slides also now have the same problem, either crystals if washed with tap water, or poor eosin staining if rinsed with MilliQ water. Our next step is to examine the slides microscopically at every step and try to find at which step the problem is occurring. Any thoughts or similar experiences? E. Wayne Johnson, DVM Enruikang AgTech MOA Feed Industry Centre Beijing From richard.wild <@t> wanadoo.fr Tue Aug 21 01:48:15 2012 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Tue Aug 21 01:48:18 2012 Subject: [Histonet] help ! Dako Seymour labeling software - autostainer plus Message-ID: <50332F2F.8070508@wanadoo.fr> Hello dear histoneters First let me thank you all for your help for lost password (autostainer plus software) - "a" - "a" tip is ok. I am begining to test the autostainer and now eveything seems fine (there was a stuck tube but I could settle the problem) _Corrupted files : Dako Seymour software (TLP 2742 printer)_ Autostainer plus can print labels directly from main software. But printing labels can be done from another computer if you use a another provided software (Dako Seymour labeling software) My software dako Seymour 5.0 is on two floppies and two compressed files are corrupted */VB40016.DL_/* (on first floppy) and */ARW01DN0.IC_/* (on second floppy) _It would be great if someone could send me by mail the two missing working files_ (compressed format, exactly as I have written them here would be better, but may be uncompressed files that are in the computer could work - these files are not very heavy and can easily be sent) I asked Dako France but they dont have anymore the floppies of the Dako Seymour labeling software (5.0) _Does someone have a list or a link of best protocols (procedures) for the machine ?_ have a nice day Best regards R Wild From DKBoyd <@t> chs.net Tue Aug 21 06:57:13 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Aug 21 06:57:23 2012 Subject: [Histonet] RE: Productivity In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB7624639D8D03C@TN001WEXMBX12.US.chs.net> Our unit of measure is billable test. But, my director adds in extra (not sure how) for manual vs. automation in the clinical lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, August 20, 2012 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Productivity Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From HornHV <@t> archildrens.org Tue Aug 21 07:15:50 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Aug 21 07:15:58 2012 Subject: [Histonet] RE: Formalin and Operating Rooms In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A17CC@EVS1.archildrens.org> Debra and others: All of our specimens are sent fresh because surgery says formalin cannot be in the operating rooms. I'm told it comes from AORN, which is the association for operating room nurses. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Monday, August 20, 2012 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Operating Rooms Hi All, I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From ihcman2010 <@t> hotmail.com Tue Aug 21 07:33:39 2012 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Tue Aug 21 07:33:45 2012 Subject: [Histonet] Renal Cell Carcinoma Poll In-Reply-To: <1345501386.59218.YahooMailNeo@web125406.mail.ne1.yahoo.com> References: <1345501386.59218.YahooMailNeo@web125406.mail.ne1.yahoo.com> Message-ID: All, What is your favorite Renal Cell Carcinoma Antibody? I'm using the Leica Bond stainer with Leica's detection & am looking for the best fit. In other words, the antibody that I am using is working, but I want it to look better. Any responses would be greatly appreciated. Glen Dawson BS, HT(ASCP), QIHC IHC Technical Specialist Janesville, WI From billodonnell <@t> catholichealth.net Tue Aug 21 08:05:23 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Aug 21 08:05:42 2012 Subject: [Histonet] Formalin and Operating Rooms In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From LPaveli1 <@t> hurleymc.com Tue Aug 21 08:35:56 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Aug 21 08:36:15 2012 Subject: [Histonet] Formalin and Operating Rooms In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> References: , <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D50E@EXCHANGEMB1.hmc.hurleymc.com> I also have not heard of any recent rule changes. Being aware of the formalin mishap in OR, our hospital has been using for over 20 years, a light blue tinged 10%NBF that the supplier makes up special. The blue is actually just a few drops of food coloring that the manufacturer adds, and the blue does NOT carry over through processing and staining. I am also aware that we are not the only hospital using this blue formalin. There is no confusion for the OR personnel when they grab the appropriate container (NBF v/s saline). hope this helps, Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of O'Donnell, Bill [billodonnell@catholichealth.net] Sent: Tuesday, August 21, 2012 9:05 AM To: Paula Sicurello; Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Operating Rooms I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Tue Aug 21 09:02:17 2012 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Aug 21 09:02:22 2012 Subject: [Histonet] DAB precipitate on H. pylori In-Reply-To: References: <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DF28025AD@FWDCWPMSGCMS09.hca.corpad.net> We never had a problem until they changed AB's on us. We sent them slides procedures etc to test but in the mean time we tried Cell Marques AB and it was clean. Problem solved. Now we all need to demand that Ventana switch back to Cell Marques H pylori so that we do not have to pay for those #@*&% prep kits. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, August 20, 2012 2:11 PM To: Dorothy Glass; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB precipitate on H. pylori Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Monday, August 20, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB precipitate on H. pylori I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Aug 21 09:40:37 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Aug 21 09:40:50 2012 Subject: [Histonet] Position - Snr Histotechnician Message-ID: We have a vacancy for a Senior Histotechnican. The Senior Histotechnician is responsible for performing a wide variety of histotechnological testing including, but not limited to, microtomy, cryotomy, complex special stains, IF, IHC, enzyme histochemistry and electron microscopy. The potential exists to become involved in research projects within the department. A working knowledge of Lean principles is useful. Theoretical knowledge as well as high quality practical ability is required. Candidates with expertise in Immunohistochemistry and/or Electron Microscopy preferred. Experience in pediatrics desirable. Registered HT(ASCP) or equivalent, with a minimum of 5 years histopathology experience in a similar laboratory Please apply on our web-site, and/or contact Sue Doud (614-355-41-5) or myself for more details Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. 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From brett_connolly <@t> merck.com Tue Aug 21 09:55:48 2012 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Aug 21 09:56:10 2012 Subject: [Histonet] Formalin and Operating Rooms In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> Message-ID: Here is a notorious case from my days working in a Ft. Lauderdale suburban hospital. Along with Bill's experiences we took formalin out of the OR after this was reported, but it wasn't a requirement as far as I remember. http://www.nytimes.com/1985/03/10/us/one-death-many-questions-in-miami.html?pagewanted=all Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, August 21, 2012 9:05 AM To: Paula Sicurello; Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Operating Rooms I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From ewj <@t> pigsqq.org Tue Aug 21 10:08:47 2012 From: ewj <@t> pigsqq.org (E. Wayne Johnson) Date: Tue Aug 21 10:09:36 2012 Subject: [Histonet] annoying crystals on sections In-Reply-To: References: Message-ID: <5033A47F.80807@pigsqq.org> we are using a Sakura DRS2000 and we are 3 x 3 minutes in xylene and we have been keeping it fresh. The staining is good now but we still see the crystals. If it were paraffin we should see unstained spots on the slide I think. I have gone to an aqueous 1% HCl today after hematoxylin for regression and that seems to be cleaning them up on most of the slides. I cleaned out some of the plumbing and cleaned some calcium out of the pipes. We are using "Harris hematoxylin" that we purchase. We have a tried different counterstains but it seems to make no difference. We are using a Sakura tissue processor for overnight processing of cassettes. the embedding is going good and we get nice flat thin sections. We are fixing tissues with neutral phosphate buffered formalin but still see some formalin pigment. We are cleaning that up with picric acid in etoh. We find we still need that and the formalin pigment is brown to dark brown. These problem crystals are round irregular to rhomboidal some times sort of large and flat about the size of a cell and they are clear. I thought they were formalin pigment at first and fiddled with the Picric Acid, and tried Ammonia in Alcohol to get rid of formalin pigment and finally decided that it was not formalin pigment. I thought it might be something from Scott's tap water (Mg++) so i dropped that and tried bluing with NH4+ and it didnt help any. I tried blueing just with tap water. Nice result but still the crystals. The aqueous HCl seems to be working and is not harming the nuclei so I may have a sort of solution and am calling it calcium crystals in the water until I know better. I may look for some sort of filter to put in the water line. E Wayne Johnson DVM Enruikang Ag Tech MOA Feed Industry Centre China Agriculture University Beijing On 8/21/2012 7:51 PM, Debra Siena wrote: > could it be paraffin? > Debbie Siena HT(ASCP)QIHC > Technical Manager | StatLab Medical Products > 407 Interchange St. | McKinney, TX 75071 > Direct: 972-436-1010 x229 | Fax: 972-436-1369 > dsiena@statlab.com | www.statlab.com > > > ----- Original Message ----- > From: ewj@pigsqq.org [mailto:ewj@pigsqq.org] > Sent: Tuesday, August 21, 2012 05:46 AM > To: Debra Siena > Subject: Re: [Histonet] annoying crystals on sections > > We just now ran the statlab version protocol from StatLab's website, using an alcoholic eosin. No doubt that gives a stronger brighter red stain. However we still see those crystals! > > We are suspecting a water problem. I have been dismantling some of the plumbing and getting some Ca crystals out of the pipes. > > We are manually coverslipping. > > >> -------Original Message------- >> From: Debra Siena >> To: 'ewj@pigsqq.org' >> Subject: Re: [Histonet] annoying crystals on sections >> Sent: Aug 21 '12 07:46 >> >> Is your eosin alcoholic or aqueous? What is your staining protocol and what reagents are you using? This information would be most helpful. Also are the sections paraffin embedded and routinely processed in tissue processor? >> Debbie Siena HT(ASCP)QIHC >> Technical Manager | StatLab Medical Products >> 407 Interchange St. | McKinney, TX 75071 >> Direct: 972-436-1010 x229 | Fax: 972-436-1369 >> dsiena@statlab.com | www.statlab.com >> >> >> ----- Original Message ----- >> From: E. Wayne Johnson [mailto:ewj@pigsqq.org] >> Sent: Monday, August 20, 2012 06:20 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] annoying crystals on sections >> >> We are having problems with crystals precipitated on our slides which >> are H&E stains on tissues from pigs. >> Tissues are fixed in buffered formalin. We had trouble months ago with >> formalin pigment and we had resolved >> that by using ammonia in EtOH or picric acid in EtOH. Sometimes we >> receive fixed samples from the field that are >> not buffered but presently all of our tissues are fixed in neutral >> phosphate buffered formalin. >> >> We moved the Sakura autostainer to a different location under a fume >> hood on a >> different floor of the building to get the solvent odor out of our work >> area. >> Immediately we began to see a tremendous degradation in slide quality >> due to what we initially thought was formalin pigment. >> We have changed all of the solutions and all of the stains. We find >> that if we use Milli-q water instead of tap water for >> rinsing (done by hand in that case) we dont see the crystals, but the >> eosin staining quality is not acceptable after rinsing in the acidic >> (ph ~5) Milli-Q water. >> >> Our tap water is neutral to slightly alkaline and is very hard with calcium. >> >> We do all sorts of tissues for diagnosis of pig diseases. Sometimes the >> slides are quite acceptable but sometimes particularly >> when looking at small intestine, the crystals are very annoying. The >> crystals occur randomly on the slide except that there is a >> tendency for them to be centered on nuclei particularly in intestinal >> epithelium. The crystals are birefringent in polarized light but >> seem to be generally clear not dark like the formalin pigment we had >> seen before. Neither ammonia nor picric acid remove these, >> and now if we use alcoholic ammonia to treat the slides, the slides come >> out too blue. Our slides are cut at 4 to 5 microns. >> >> Brain has the least problem, small intestine seems worst. >> >> We have gone back and cut some blocks that previously stained >> beautifully with no pigment or precipitate problems >> and those slides also now have the same problem, either crystals if >> washed with tap water, or poor eosin staining if rinsed with MilliQ water. >> >> Our next step is to examine the slides microscopically at every step and >> try to find at which step the problem is occurring. >> >> Any thoughts or similar experiences? >> >> E. Wayne Johnson, DVM >> Enruikang AgTech >> MOA Feed Industry Centre >> Beijing >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > From ttruscot <@t> vetmed.wsu.edu Tue Aug 21 11:49:20 2012 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Tue Aug 21 11:49:30 2012 Subject: [Histonet] annoying crystals on sections In-Reply-To: <5033A47F.80807@pigsqq.org> References: <5033A47F.80807@pigsqq.org> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00272E1191AE@CVM76.vetmed.wsu.edu> Hi Wayne, I had lots of problems with round irregular "crystals", but have greatly improved my slides by limiting baking at 57 degrees to 1/2 hour. We use a paraffin with plastic in the mix, and I think the plastic globs up under some conditions, like no or not enough xylene to dissolve it out. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: Tuesday, August 21, 2012 8:09 AM To: Debra Siena; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] annoying crystals on sections we are using a Sakura DRS2000 and we are 3 x 3 minutes in xylene and we have been keeping it fresh. The staining is good now but we still see the crystals. If it were paraffin we should see unstained spots on the slide I think. I have gone to an aqueous 1% HCl today after hematoxylin for regression and that seems to be cleaning them up on most of the slides. I cleaned out some of the plumbing and cleaned some calcium out of the pipes. We are using "Harris hematoxylin" that we purchase. We have a tried different counterstains but it seems to make no difference. We are using a Sakura tissue processor for overnight processing of cassettes. the embedding is going good and we get nice flat thin sections. We are fixing tissues with neutral phosphate buffered formalin but still see some formalin pigment. We are cleaning that up with picric acid in etoh. We find we still need that and the formalin pigment is brown to dark brown. These problem crystals are round irregular to rhomboidal some times sort of large and flat about the size of a cell and they are clear. I thought they were formalin pigment at first and fiddled with the Picric Acid, and tried Ammonia in Alcohol to get rid of formalin pigment and finally decided that it was not formalin pigment. I thought it might be something from Scott's tap water (Mg++) so i dropped that and tried bluing with NH4+ and it didnt help any. I tried blueing just with tap water. Nice result but still the crystals. The aqueous HCl seems to be working and is not harming the nuclei so I may have a sort of solution and am calling it calcium crystals in the water until I know better. I may look for some sort of filter to put in the water line. E Wayne Johnson DVM Enruikang Ag Tech MOA Feed Industry Centre China Agriculture University Beijing On 8/21/2012 7:51 PM, Debra Siena wrote: > could it be paraffin? > Debbie Siena HT(ASCP)QIHC > Technical Manager | StatLab Medical Products > 407 Interchange St. | McKinney, TX 75071 > Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | > www.statlab.com > > > ----- Original Message ----- > From: ewj@pigsqq.org [mailto:ewj@pigsqq.org] > Sent: Tuesday, August 21, 2012 05:46 AM > To: Debra Siena > Subject: Re: [Histonet] annoying crystals on sections > > We just now ran the statlab version protocol from StatLab's website, using an alcoholic eosin. No doubt that gives a stronger brighter red stain. However we still see those crystals! > > We are suspecting a water problem. I have been dismantling some of the plumbing and getting some Ca crystals out of the pipes. > > We are manually coverslipping. > > >> -------Original Message------- >> From: Debra Siena >> To: 'ewj@pigsqq.org' >> Subject: Re: [Histonet] annoying crystals on sections >> Sent: Aug 21 '12 07:46 >> >> Is your eosin alcoholic or aqueous? What is your staining protocol and what reagents are you using? This information would be most helpful. Also are the sections paraffin embedded and routinely processed in tissue processor? >> Debbie Siena HT(ASCP)QIHC >> Technical Manager | StatLab Medical Products >> 407 Interchange St. | McKinney, TX 75071 >> Direct: 972-436-1010 x229 | Fax: 972-436-1369 >> dsiena@statlab.com | www.statlab.com >> >> >> ----- Original Message ----- >> From: E. Wayne Johnson [mailto:ewj@pigsqq.org] >> Sent: Monday, August 20, 2012 06:20 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] annoying crystals on sections >> >> We are having problems with crystals precipitated on our slides which >> are H&E stains on tissues from pigs. >> Tissues are fixed in buffered formalin. We had trouble months ago with >> formalin pigment and we had resolved >> that by using ammonia in EtOH or picric acid in EtOH. Sometimes we >> receive fixed samples from the field that are >> not buffered but presently all of our tissues are fixed in neutral >> phosphate buffered formalin. >> >> We moved the Sakura autostainer to a different location under a fume >> hood on a >> different floor of the building to get the solvent odor out of our work >> area. >> Immediately we began to see a tremendous degradation in slide quality >> due to what we initially thought was formalin pigment. >> We have changed all of the solutions and all of the stains. We find >> that if we use Milli-q water instead of tap water for >> rinsing (done by hand in that case) we dont see the crystals, but the >> eosin staining quality is not acceptable after rinsing in the acidic >> (ph ~5) Milli-Q water. >> >> Our tap water is neutral to slightly alkaline and is very hard with calcium. >> >> We do all sorts of tissues for diagnosis of pig diseases. Sometimes the >> slides are quite acceptable but sometimes particularly >> when looking at small intestine, the crystals are very annoying. The >> crystals occur randomly on the slide except that there is a >> tendency for them to be centered on nuclei particularly in intestinal >> epithelium. The crystals are birefringent in polarized light but >> seem to be generally clear not dark like the formalin pigment we had >> seen before. Neither ammonia nor picric acid remove these, >> and now if we use alcoholic ammonia to treat the slides, the slides come >> out too blue. Our slides are cut at 4 to 5 microns. >> >> Brain has the least problem, small intestine seems worst. >> >> We have gone back and cut some blocks that previously stained >> beautifully with no pigment or precipitate problems >> and those slides also now have the same problem, either crystals if >> washed with tap water, or poor eosin staining if rinsed with MilliQ water. >> >> Our next step is to examine the slides microscopically at every step and >> try to find at which step the problem is occurring. >> >> Any thoughts or similar experiences? >> >> E. Wayne Johnson, DVM >> Enruikang AgTech >> MOA Feed Industry Centre >> Beijing >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Aug 21 11:52:15 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Aug 21 11:52:18 2012 Subject: [Histonet] annoying crystals on sections In-Reply-To: <5033A47F.80807@pigsqq.org> References: <5033A47F.80807@pigsqq.org> Message-ID: Obviously, it's your water, if you don't see the crystals after changing your water. If the acidic pH of the Milli-q water is leaching out your eosin, then adjust the pH of the water to 7.0 before using it to rinse your slides. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From jaylundgren <@t> gmail.com Tue Aug 21 12:25:00 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Aug 21 12:25:05 2012 Subject: [Histonet] Formalin and Operating Rooms In-Reply-To: References: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> Message-ID: "Johnson & Johnson just a few days ago announced that they're going to take formaldehyde out of baby shampoo!" No wonder I can't get my baby's hair squeeky clean anymore! How much formalin do I need to add to replace the formalin they took out? Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) From monishapahuja <@t> gmail.com Tue Aug 21 12:32:22 2012 From: monishapahuja <@t> gmail.com (Nisha) Date: Tue Aug 21 12:32:28 2012 Subject: [Histonet] Accessions/ lean Message-ID: My facility currently takes specimens out of plastic bags, accessions the case, and then stuffs the specimens back into the plastic bags. One of the residents mentioned that other places put the specimens into plastic bins for the grossers. I have been asked to evaluate the feasability of this, but I have never seen it done, don't know how the bins would stack, how many different sizes would be needed, how much room it would take up, etc. If anyone that does this can comment or better yet could provide a photo(s) of the bin set-up at accessioning/grossing that would be great. Thanks in advance!!" Nisha Lead histology technian Robert Wood Johnson University Hospital Sent from my iPhone From joelleweaver <@t> hotmail.com Tue Aug 21 13:12:24 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Aug 21 13:12:32 2012 Subject: [Histonet] Training -LIS Message-ID: Hello HistonettersI am wondering if anyone can direct me to some good training courses or sources for IT training, but specifically in areas that would be of good use for LIS. I have taken some health informatics courses with SQL, some LOINC,and EMR topics and I am looking to get into these studies a little further. I am not really looking to go into a formal degree program at this time, I am wanting to learn more but only as a part-time study venture. Thanks for your input.Joelle Joelle Weaver MAOM, HTL (ASCP) QIHC From tkngflght <@t> yahoo.com Tue Aug 21 13:29:23 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Aug 21 13:29:26 2012 Subject: [Histonet] Open jobs - including Supervisor & Manager Message-ID: <1345573763.68386.YahooMailNeo@web39401.mail.mud.yahoo.com> Hello my fellow 'netters! We have another bunch of openings-- Routine bench-- ????? Day, afternoon and night shift -- all over the country and registered and non-registered openings are available. Supervisor- ????? Several?openings includind a night shift position Manager- ????? One big?job and several solo positions.? Call for more information-- be ready with your most recent resume and some will require education transcripts even if you've not finished the degree....? If what we have doesn't suit, we'll go looking for you! The conversation is with me, a working histotech--so it is quick and usually kinda fun. Thank you! ?In service! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From rjbuesa <@t> yahoo.com Tue Aug 21 14:51:50 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 21 14:51:55 2012 Subject: [Histonet] Accessions/ lean In-Reply-To: References: Message-ID: <1345578710.56793.YahooMailNeo@web121406.mail.ne1.yahoo.com> Regardless of what you end doing, NEVER "touch" anything twice to return to the same container it came. The idea is that every time you "touch" a specimen you "add value to it". This means that the specimen should "fall into a chain of work", into a "forward flow" that makes the specimen one step closer to finish every time you handle it. Taken a specimen from a plastic bag to put it AGAIN into the same plastic bag is "anathema" from the Lean point of view. Study your flow and try that your specimen keeps "marching forward" with every step you subject it to. Ren? J. ________________________________ From: Nisha To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, August 21, 2012 1:32 PM Subject: [Histonet] Accessions/ lean My facility currently takes specimens out of plastic bags, accessions the case, and then stuffs the specimens back into the plastic bags.? One of the residents mentioned that other places put the specimens into plastic bins for the grossers.? I have been asked to evaluate the feasability of this, but I have never seen it done, don't know how the bins would stack, how many different sizes would be needed, how much room it would take up, etc. If anyone that does this can comment or better yet could provide a photo(s) of the bin set-up at accessioning/grossing that would be great.? Thanks in advance!!" Nisha Lead histology technian Robert Wood Johnson University Hospital Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkrichar <@t> gundluth.org Tue Aug 21 14:52:50 2012 From: pkrichar <@t> gundluth.org (Richardson, Pam K) Date: Tue Aug 21 14:53:01 2012 Subject: [Histonet] Gloves Message-ID: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> Hi, what type of gloves are you wearing when handling xylene? Do you double glove? Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org From rjbuesa <@t> yahoo.com Tue Aug 21 14:55:55 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 21 14:56:01 2012 Subject: [Histonet] Gloves In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> Message-ID: <1345578955.27732.YahooMailNeo@web121406.mail.ne1.yahoo.com> The best type is the?one used to handle paint removers ("paint thinners"). Ren? J. ________________________________ From: "Richardson, Pam K" To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, August 21, 2012 3:52 PM Subject: [Histonet] Gloves Hi, what type of gloves are you wearing when handling xylene? Do you double glove? Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Tue Aug 21 14:50:45 2012 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Aug 21 14:57:48 2012 Subject: [Histonet] Accessions/ lean In-Reply-To: References: Message-ID: We use baskets here. They get them from office depot. Put each case in one basket and then put on a cart to deliver to the grosser. For big specimens that don't fit we just set the container on the grossing table. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nisha Sent: Tuesday, August 21, 2012 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Accessions/ lean My facility currently takes specimens out of plastic bags, accessions the case, and then stuffs the specimens back into the plastic bags. One of the residents mentioned that other places put the specimens into plastic bins for the grossers. I have been asked to evaluate the feasability of this, but I have never seen it done, don't know how the bins would stack, how many different sizes would be needed, how much room it would take up, etc. If anyone that does this can comment or better yet could provide a photo(s) of the bin set-up at accessioning/grossing that would be great. Thanks in advance!!" Nisha Lead histology technian Robert Wood Johnson University Hospital Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Tue Aug 21 15:00:00 2012 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Tue Aug 21 15:00:04 2012 Subject: [Histonet] RE: Gloves and Xylene In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> Message-ID: For ordinary applications like manual staining, nitrile gloves work fine. I don't double glove, just change gloves ad lib. Jerry > From: pkrichar@gundluth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 21 Aug 2012 19:52:50 +0000 > Subject: [Histonet] Gloves > > Hi, what type of gloves are you wearing when handling xylene? Do you double glove? > > Cordially, > > Pam ~ > > ++++++++++++++++++++++++++++ > Pam Richardson > Clinical Manager > Gundersen Lutheran Laboratory > Email: pkrichar@gundluth.org > Phone: 608 775-4133 > Fax: 608 775-6136 > Interdepartmental Mail Stop: H04-007 > E-visit us at: http://www.gundluth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Tue Aug 21 15:03:29 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Aug 21 15:03:34 2012 Subject: [Histonet] Gloves Message-ID: <5033E991.5050604@mclean.harvard.edu> Hi Pam: I use nitrile gloves that we get from our Central Supply Room when handling xylene and cover-slipping by hand. They are Kimberly-Clark Sterling Nitrile Powder-Free Exam Gloves. I use just a single glove rather than double-gloving, and that seems to do the trick. The glove usually does not break. However, now that you mention it, I wonder if a single layer really totally prevents penetration of xylene or not. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 02478 From ncosenza <@t> siumed.edu Tue Aug 21 15:06:04 2012 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Tue Aug 21 15:06:07 2012 Subject: [Histonet] cryosections with DiD labeling Message-ID: <5033EA2C.60006@siumed.edu> We are beginning a retrograde tracing study in rats. We injected DiD into the rats and in 4 weeks we will perfuse and harvest the brainstems. My question is, is vibratome sectioning better than cryostat sectioning? The literature that accompanied the dye said either is an option, but some have noted dye leaching out when cyrostat sectioned. How common is this problem? If vibratome is the better choice, is there a good embedding protocol? From TGoins <@t> mt.gov Tue Aug 21 15:24:48 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Aug 21 15:24:57 2012 Subject: [Histonet] RE: Gloves In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> Message-ID: We use nitrile when handling everything except acetone - then we use latex. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richardson, Pam K Sent: Tuesday, August 21, 2012 1:53 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Gloves Hi, what type of gloves are you wearing when handling xylene? Do you double glove? Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmendell <@t> goldbergmd.net Tue Aug 21 16:24:54 2012 From: kmendell <@t> goldbergmd.net (MaryK Mendell) Date: Tue Aug 21 16:25:02 2012 Subject: [Histonet] RE: Gloves In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FDEA15DD@LXEXMB03.gundluth.org> Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D341@EXMBX01.mmeprod.cbeyond> Pam I hand coverslip and have found in time most of the nitrile gloves break down and get real soft. However, by accident our purchasing department ordered SemperSure Nitrile gloves (tested for use with chemothepary drugs) and these are the only gloves that I found that don't get soft and break down. There is 200 gloves in the box and are true to size They are by Sempermed the order number for the small is SUNF202 Kate Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richardson, Pam K [pkrichar@gundluth.org] Sent: Tuesday, August 21, 2012 3:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Gloves Hi, what type of gloves are you wearing when handling xylene? Do you double glove? Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 <@t> uchicago.edu Tue Aug 21 16:27:22 2012 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Tue Aug 21 16:27:32 2012 Subject: [Histonet] Re: annoying crystals on sections Message-ID: <20120821162722.BND01141@mstore03.uchicago.edu> Hi Wayne & Histonet My guess is that Wayne's crystals are Calcium phosphate - the calcium from the hard tap water, as described, and phosphate from phosphate buffer (sodium or potassium) in the solution immediately preceding the tapwater rinse. The variation in crystal deposition would then be in the degree each tissue/ organelle tends to carry over the phosphate into the tapwater wash. Just mix drops of the solutions together on a slide and see if crystals form. A brief deionized rinse followed by tapwater should first remove the phosphate (& crystals) and then allow the desired blueing. Alternatively, substitute TRIS or similar as the buffer in the preceding step and go directly to tapwater. If you have valuable sections with crystals on them, you should be able to chelate away the deposits in an EDTA solution, then restain as needed. all the best! -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ---- Original message ---- >Date: Tue, 21 Aug 2012 09:44:26 -0500 (CDT) >Subject: Histonet Digest, Vol 105, Issue 25 Message: 12 >Date: Tue, 21 Aug 2012 07:20:06 +0800 >From: "E. Wayne Johnson" >Subject: [Histonet] annoying crystals on sections > >We are having problems with crystals precipitated on our slides which are H&E stains on tissues from pigs. Tissues are fixed in buffered formalin. We had trouble months ago with formalin pigment and we had resolved that by using ammonia in EtOH or picric acid in EtOH. Sometimes we receive fixed samples from the field that are not buffered but presently all of our tissues are fixed in neutral phosphate buffered formalin. > >We moved the Sakura autostainer to a different location under a fume hood on a different floor of the building to get the solvent odor out of our work area. >Immediately we began to see a tremendous degradation in slide quality due to what we initially thought was formalin pigment. >We have changed all of the solutions and all of the stains. We find that if we use Milli-q water instead of tap water for >rinsing (done by hand in that case) we don't see the crystals, but the eosin staining quality is not acceptable after rinsing in the acidic (ph ~5) Milli-Q water. > >Our tap water is neutral to slightly alkaline and is very hard with calcium. > >We do all sorts of tissues for diagnosis of pig diseases. Sometimes the slides are quite acceptable but sometimes particularly when looking at small intestine, the crystals are very annoying. The crystals occur randomly on the slide except that there is a tendency for them to be centered on nuclei particularly in intestinal epithelium. The crystals are birefringent in polarized light but seem to be generally clear not dark like the formalin pigment we had seen before. Neither ammonia nor picric acid remove these, and now if we use alcoholic ammonia to treat the slides, the slides come out too blue. Our slides are cut at 4 to 5 microns. > >Brain has the least problem, small intestine seems worst. > >We have gone back and cut some blocks that previously stained beautifully with no pigment or precipitate problems >and those slides also now have the same problem, either crystals if washed with tap water, or poor eosin staining if rinsed with MilliQ water. > >Our next step is to examine the slides microscopically at every step and try to find at which step the problem is occurring. > >Any thoughts or similar experiences? > >E. Wayne Johnson, DVM >Enruikang AgTech >MOA Feed Industry Centre >Beijing >------------------------------ From ewj <@t> pigsqq.org Tue Aug 21 18:12:55 2012 From: ewj <@t> pigsqq.org (E. Wayne Johnson) Date: Tue Aug 21 18:13:41 2012 Subject: [Histonet] Re: annoying crystals on sections In-Reply-To: <20120821162722.BND01141@mstore03.uchicago.edu> References: <20120821162722.BND01141@mstore03.uchicago.edu> Message-ID: <503415F7.9000301@pigsqq.org> Thanks all for many useful and helpful suggestions and interesting anecdotes. E. Wayne Johnson, DVM Enruikang AgTech MOA Feed Industry Centre China Agricultural University Beijing On 8/22/2012 5:27 AM, David A. Wright wrote: > Hi Wayne& Histonet > > My guess is that Wayne's crystals are Calcium phosphate - the calcium from the hard tap water, as described, and phosphate from phosphate buffer (sodium or potassium) in the solution immediately preceding the tapwater rinse. The variation in crystal deposition would then be in the degree each tissue/ organelle tends to carry over the phosphate into the tapwater wash. Just mix drops of the solutions together on a slide and see if crystals form. > > A brief deionized rinse followed by tapwater should first remove the phosphate (& crystals) and then allow the desired blueing. Alternatively, substitute TRIS or similar as the buffer in the preceding step and go directly to tapwater. > > If you have valuable sections with crystals on them, you should be able to chelate away the deposits in an EDTA solution, then restain as needed. > all the best! > -David > == > David A. Wright, Ph.D. > University of Chicago Section of Neurosurgery > > ---- Original message ---- > >> Date: Tue, 21 Aug 2012 09:44:26 -0500 (CDT) >> Subject: Histonet Digest, Vol 105, Issue 25 Message: 12 >> Date: Tue, 21 Aug 2012 07:20:06 +0800 >> From: "E. Wayne Johnson" >> Subject: [Histonet] annoying crystals on sections >> >> We are having problems with crystals precipitated on our slides which are H&E stains on tissues from pigs. Tissues are fixed in buffered formalin. We had trouble months ago with formalin pigment and we had resolved that by using ammonia in EtOH or picric acid in EtOH. Sometimes we receive fixed samples from the field that are not buffered but presently all of our tissues are fixed in neutral phosphate buffered formalin. >> >> We moved the Sakura autostainer to a different location under a fume hood on a different floor of the building to get the solvent odor out of our work area. >> Immediately we began to see a tremendous degradation in slide quality due to what we initially thought was formalin pigment. >> We have changed all of the solutions and all of the stains. We find that if we use Milli-q water instead of tap water for >> rinsing (done by hand in that case) we don't see the crystals, but the eosin staining quality is not acceptable after rinsing in the acidic (ph ~5) Milli-Q water. >> >> Our tap water is neutral to slightly alkaline and is very hard with calcium. >> >> We do all sorts of tissues for diagnosis of pig diseases. Sometimes the slides are quite acceptable but sometimes particularly when looking at small intestine, the crystals are very annoying. The crystals occur randomly on the slide except that there is a tendency for them to be centered on nuclei particularly in intestinal epithelium. The crystals are birefringent in polarized light but seem to be generally clear not dark like the formalin pigment we had seen before. Neither ammonia nor picric acid remove these, and now if we use alcoholic ammonia to treat the slides, the slides come out too blue. Our slides are cut at 4 to 5 microns. >> >> Brain has the least problem, small intestine seems worst. >> >> We have gone back and cut some blocks that previously stained beautifully with no pigment or precipitate problems >> and those slides also now have the same problem, either crystals if washed with tap water, or poor eosin staining if rinsed with MilliQ water. >> >> Our next step is to examine the slides microscopically at every step and try to find at which step the problem is occurring. >> >> Any thoughts or similar experiences? >> >> E. Wayne Johnson, DVM >> Enruikang AgTech >> MOA Feed Industry Centre >> Beijing >> ------------------------------ >> > > From Jonathan.Cremer <@t> med.kuleuven.be Wed Aug 22 01:12:12 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Aug 22 01:12:27 2012 Subject: [Histonet] Gloves In-Reply-To: <5033E991.5050604@mclean.harvard.edu> References: <5033E991.5050604@mclean.harvard.edu> Message-ID: Xylene will penetrate nitrile gloves within minutes, so it's good practice to change gloves when they're wetted with xylene. You can check this yourself by sniffing your hands after pulling off gloves contaminated with xylene, you'll know if a 'considerable' amount of xylene has come through. --- Jonathan Cremer ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Tim Wheelock [twheelock@mclean.harvard.edu] Verzonden: dinsdag 21 augustus 2012 22:03 To: 'histonet@lists.utsouthwestern.edu' Onderwerp: [Histonet] Gloves Hi Pam: I use nitrile gloves that we get from our Central Supply Room when handling xylene and cover-slipping by hand. They are Kimberly-Clark Sterling Nitrile Powder-Free Exam Gloves. I use just a single glove rather than double-gloving, and that seems to do the trick. The glove usually does not break. However, now that you mention it, I wonder if a single layer really totally prevents penetration of xylene or not. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 02478 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Thomas.Crowell <@t> MPI.com Wed Aug 22 08:13:25 2012 From: Thomas.Crowell <@t> MPI.com (Crowell, Thomas) Date: Wed Aug 22 08:14:05 2012 Subject: [Histonet] Leica Bond Stainer In-Reply-To: <6751b716-a35f-48fa-ac73-ec6b4514b511@exch-cas01prd.corp.mpi.com> References: <6751b716-a35f-48fa-ac73-ec6b4514b511@exch-cas01prd.corp.mpi.com> Message-ID: <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> Hello all, Our laboratory is considering a purchase, and Leica will be giving a presentation on the unit in September. I was hoping to get some responses of pros/cons/quirks from users to help in our assessment. We are currently using Ventana XT to look at PD biomarkers in cell lines, xenografts and some clinical samples. I would be interested in a comparison of performance and reliability between these platforms. Any responses would be greatly appreciated. Thanks! Tom Thomas Crowell Supplemental Contractor Molecular Pathology Millennium: The Takeda Oncology Company 35 Landsdowne Street | Cambridge, MA | 02139 Phone: 617.444.2303 Fax: 617.551.3744 thomas.crowell@mpi.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, August 21, 2012 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Productivity (Heckford, Karen - SMMC-SF) 2. Ventana Symphony reagents (Clare Thornton) 3. DAB precipitate on H. pylori (Dorothy Glass) 4. RE: DAB precipitate on H. pylori (Vanessa Perez) 5. RE: Productivity (CHRISTIE GOWAN) 6. Re: Productivity (Rene J Buesa) 7. Re: Histonet Digest, Vol 105, Issue 24 (Me) 8. New Position (Wilson A) 9. Formalin and Operating Rooms (Debra Siena) 10. Re: Formalin and Operating Rooms (Mark Tarango) 11. Re: Formalin and Operating Rooms (Paula Sicurello) 12. annoying crystals on sections (E. Wayne Johnson) 13. help ! Dako Seymour labeling software - autostainer plus (richard wild) 14. RE: Productivity (Boyd, Debbie M) 15. RE: Formalin and Operating Rooms (Horn, Hazel V) 16. Renal Cell Carcinoma Poll (Glen Dawson) 17. RE: Formalin and Operating Rooms (O'Donnell, Bill) 18. RE: Formalin and Operating Rooms (Lynette Pavelich) 19. RE: DAB precipitate on H. pylori (Susan.Walzer@HCAHealthcare.com) 20. Position - Snr Histotechnician (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Aug 2012 10:42:39 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Productivity To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 2 Date: Mon, 20 Aug 2012 13:49:25 -0400 From: Clare Thornton Subject: [Histonet] Ventana Symphony reagents To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have some Symphony reagents and coverslips that we no longer need. If you are in the New England area, we could deliver them with our courier service. If you are outside of New England, if you pay the shipping we will send them. Please email me off list if you would like to know what we have and are interested in taking any/all of it. Have a great day everyone! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 3 Date: Mon, 20 Aug 2012 11:12:50 -0700 (PDT) From: Dorothy Glass Subject: [Histonet] DAB precipitate on H. pylori To: histonet@lists.utsouthwestern.edu Message-ID: <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? ------------------------------ Message: 4 Date: Mon, 20 Aug 2012 13:10:40 -0500 From: Vanessa Perez Subject: RE: [Histonet] DAB precipitate on H. pylori To: Dorothy Glass , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Monday, August 20, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB precipitate on H. pylori I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 20 Aug 2012 18:37:55 +0000 From: CHRISTIE GOWAN Subject: RE: [Histonet] Productivity To: , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Karen, The best way to communicate with management is through data. If you have access to say the last 3 years of blocks and or slides cut and you compare it to accessions or cpt codes then you have a roadmap of where you have been in the past and where you are now. Do you have more or less employees and is this a temporary lull in numbers or has this dip occurred before. At least you will be able to formulate a more informed response to their request for cutting back hours. This is always a complicated issue and we have seen an increase in case complexity as well in our institution so our numbers of blocks vs. specimen count is skewed as well. Tell them you will cut hours but you will need all new equipment to continue to meet your deadlines! Ha!. Let us know how it turns out and good luck! Christie Gowan, HT (ASCP) UAB Hospital Histology Supervisor Surgical Pathology 205 934 4991 cgowan@uabmc.edu > From: Karen.Heckford@DignityHealth.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 20 Aug 2012 10:42:39 -0700 > Subject: [Histonet] Productivity > > Good Morning, > I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 20 Aug 2012 14:03:33 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Productivity To: "Heckford, Karen - SMMC-SF" , "histonet@lists.utsouthwestern.edu" Message-ID: <1345496613.86112.YahooMailNeo@web121401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Karen: "Productivity" and its measures depends on who is going to use it. 1- For the pathologists as a whole they are interested in CASES they receive and sign either on a daily or monthly basis; 2- For management it is measured in how many cases the pathologists can sign and therefore the administration can bill/collect 3- as the Lead Tech you are interested is the work output of your staff specially how many blocks each can section either per hour or per shift or how many minutes it takes to complete a FS. Mind that the ONLY productivity rate defined by CAP is the time a FS can be diagnosed from the moment it is received at histology and that has to be 20 minutes or less. Nowadays almost every lab has an automated stainer so the number of slides has been "downgraded" as a productivity index, although it is not the same to?prepare 1 than 20 slides. The unit of time is also a subject of debate: some prefer the "hour" but from the whole lab the index most desired is the number of CASES a lab can complete every day. Under separate cover I am sending you some articles I wrote on the subject so you can select those productivity indices most fit your lab. Ren? J. ________________________________ From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, August 20, 2012 1:42 PM Subject: [Histonet] Productivity Good Morning, I know we have been over this before, how does your lab measure productivity?? Are you going by block and slide or by CPT code?? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value.? As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides.? I do everything from GI specimens to big breast cases.? They will tell me that our productivity is down and we need to cut hours.? I just do not know exactly how to drill it in to them that things are not always as they appear on paper.? I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 20 Aug 2012 17:38:44 -0400 From: Me Subject: [Histonet] Re: Histonet Digest, Vol 105, Issue 24 To: histonet@lists.utsouthwestern.edu Message-ID: <1db21a96-209c-44f0-a517-da793748aa2c@blur> Content-Type: text/plain; Format="Flowed"; DelSp="Yes"; charset="US-ASCII" I have a question for all in Histoland... Concerning the CAP revision on the use of negative controls...I work in a NJ lab that does work for NY offices, CAP states we no longer need to run controls if using biotin free detection but does NY state board of health agree? Not running those extra slides is a huge money and time saver. We are using Leica Bond III and the polymer detection. Anyone have an answer or know where I can get the answer? Jen Elz Pathology Solutions Eatontown, NJ Sent from my Verizon Wireless Droid -----Original message----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, Aug 20, 2012 17:00:30 GMT+00:00 Subject: Histonet Digest, Vol 105, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: disposal of formalin (MaryK Mendell) 2. RE: Microtome feedback (Bea DeBrosse-Serra) 3. RE: Microtome feedback (Lynette Pavelich) 4. Immuno Tech (Carol Fields) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Aug 2012 07:29:45 -0400 From: MaryK Mendell Subject: [Histonet] RE: disposal of formalin To: "Lake,Debbie" , "histonet@lists.utsouthwestern.edu" Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D33D@EXMBX01.mmeprod.cbeyond> Content-Type: text/plain; charset="us-ascii" We also use a company to pick it up along with xylene & alcohol And you are responsible from craddle to grave. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie [Debbie.Lake@mgh.net] Sent: Friday, August 17, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 20 Aug 2012 06:55:39 -0700 From: Bea DeBrosse-Serra Subject: RE: [Histonet] Microtome feedback To: "'Ian R Bernard'" , Fred Underwood , Histonet Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34B8C@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" It is a high quality work horse. I've worked with newer and older microtomes of this very model and never had any problems. You can utilize the motorized function to minimize repetitive motion, but you can also use it manually if needed. What is there not to like? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: Ian R Bernard [mailto:ibernard@uab.edu] Sent: Saturday, August 18, 2012 3:53 AM To: Bea DeBrosse-Serra; Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback What is great about the Leica RM2255? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Friday, August 17, 2012 8:58 PM To: Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback Fred, The Leica RM2255 all the way! Go Dayton! Bea ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 20 Aug 2012 14:27:35 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Microtome feedback To: Fred Underwood , Histonet Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D3F4@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" We purchased the Leica RM2255 just over a year ago. It is the only one we have and is primarily used by one tech. In her absence, others use her machine. What is great about it, is that it is VERY easy to adapt to it. The controls are easy to figure out, cuts beautiful slides, and can be used either manually or completely automatic. Additionally, it has a "homing" option for the block holder.........pretty handy if you need to adjust the block holder often! In the future, I plan on replacing 2 more microtomes with this Leica microtome. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 3:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH ------------------------------ Message: 4 Date: Mon, 20 Aug 2012 12:20:37 -0400 From: "Carol Fields" Subject: [Histonet] Immuno Tech To: Cc: Message-ID: <731941C266951A47BEF11E5EFAAED9C911C4059C@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" If anyone has an Immuno Job Description you are willing to share I would appreciate it. My email address is carol.fields@northside.com. Thanks to all. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 24 ***************************************** ------------------------------ Message: 8 Date: Mon, 20 Aug 2012 15:23:06 -0700 (PDT) From: Wilson A Subject: [Histonet] New Position To: "histonet@lists.utsouthwestern.edu" Message-ID: <1345501386.59218.YahooMailNeo@web125406.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? ? Hi, ??? I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY. Willing to relocate. Thanks, Wilson. ------------------------------ Message: 9 Date: Mon, 20 Aug 2012 17:25:06 -0500 From: Debra Siena Subject: [Histonet] Formalin and Operating Rooms To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com ------------------------------ Message: 10 Date: Mon, 20 Aug 2012 16:04:16 -0700 From: Mark Tarango Subject: Re: [Histonet] Formalin and Operating Rooms To: Debra Siena Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Debra, Formaldehyde was listed as "known to be a human carcinogen" in the 12th Report on Carcinogens (2011) put out by the Department of Health and Human Services. Here is a link http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf This is what is probably behind any recent changes to its use or availability. Johnson & Johnson just a few days ago announced that they're going to take formaldehyde out of baby shampoo! Yah! Mark On Mon, Aug 20, 2012 at 3:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Mon, 20 Aug 2012 19:04:33 -0400 From: Paula Sicurello Subject: Re: [Histonet] Formalin and Operating Rooms To: Debra Siena Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Tue, 21 Aug 2012 07:20:06 +0800 From: "E. Wayne Johnson" Subject: [Histonet] annoying crystals on sections To: histonet@lists.utsouthwestern.edu Message-ID: <5032C626.5040206@pigsqq.org> Content-Type: text/plain; charset=ISO-8859-1; format=flowed We are having problems with crystals precipitated on our slides which are H&E stains on tissues from pigs. Tissues are fixed in buffered formalin. We had trouble months ago with formalin pigment and we had resolved that by using ammonia in EtOH or picric acid in EtOH. Sometimes we receive fixed samples from the field that are not buffered but presently all of our tissues are fixed in neutral phosphate buffered formalin. We moved the Sakura autostainer to a different location under a fume hood on a different floor of the building to get the solvent odor out of our work area. Immediately we began to see a tremendous degradation in slide quality due to what we initially thought was formalin pigment. We have changed all of the solutions and all of the stains. We find that if we use Milli-q water instead of tap water for rinsing (done by hand in that case) we dont see the crystals, but the eosin staining quality is not acceptable after rinsing in the acidic (ph ~5) Milli-Q water. Our tap water is neutral to slightly alkaline and is very hard with calcium. We do all sorts of tissues for diagnosis of pig diseases. Sometimes the slides are quite acceptable but sometimes particularly when looking at small intestine, the crystals are very annoying. The crystals occur randomly on the slide except that there is a tendency for them to be centered on nuclei particularly in intestinal epithelium. The crystals are birefringent in polarized light but seem to be generally clear not dark like the formalin pigment we had seen before. Neither ammonia nor picric acid remove these, and now if we use alcoholic ammonia to treat the slides, the slides come out too blue. Our slides are cut at 4 to 5 microns. Brain has the least problem, small intestine seems worst. We have gone back and cut some blocks that previously stained beautifully with no pigment or precipitate problems and those slides also now have the same problem, either crystals if washed with tap water, or poor eosin staining if rinsed with MilliQ water. Our next step is to examine the slides microscopically at every step and try to find at which step the problem is occurring. Any thoughts or similar experiences? E. Wayne Johnson, DVM Enruikang AgTech MOA Feed Industry Centre Beijing ------------------------------ Message: 13 Date: Tue, 21 Aug 2012 08:48:15 +0200 From: richard wild Subject: [Histonet] help ! Dako Seymour labeling software - autostainer plus To: histonet@lists.utsouthwestern.edu Message-ID: <50332F2F.8070508@wanadoo.fr> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello dear histoneters First let me thank you all for your help for lost password (autostainer plus software) - "a" - "a" tip is ok. I am begining to test the autostainer and now eveything seems fine (there was a stuck tube but I could settle the problem) _Corrupted files : Dako Seymour software (TLP 2742 printer)_ Autostainer plus can print labels directly from main software. But printing labels can be done from another computer if you use a another provided software (Dako Seymour labeling software) My software dako Seymour 5.0 is on two floppies and two compressed files are corrupted */VB40016.DL_/* (on first floppy) and */ARW01DN0.IC_/* (on second floppy) _It would be great if someone could send me by mail the two missing working files_ (compressed format, exactly as I have written them here would be better, but may be uncompressed files that are in the computer could work - these files are not very heavy and can easily be sent) I asked Dako France but they dont have anymore the floppies of the Dako Seymour labeling software (5.0) _Does someone have a list or a link of best protocols (procedures) for the machine ?_ have a nice day Best regards R Wild ------------------------------ Message: 14 Date: Tue, 21 Aug 2012 11:57:13 +0000 From: "Boyd, Debbie M" Subject: [Histonet] RE: Productivity To: "Heckford, Karen - SMMC-SF" , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB7624639D8D03C@TN001WEXMBX12.US.chs.net> Content-Type: text/plain; charset="us-ascii" Our unit of measure is billable test. But, my director adds in extra (not sure how) for manual vs. automation in the clinical lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, August 20, 2012 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Productivity Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 15 Date: Tue, 21 Aug 2012 07:15:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] RE: Formalin and Operating Rooms To: "'Debra Siena'" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A17CC@EVS1.archildrens.org> Content-Type: text/plain; charset="iso-8859-1" Debra and others: All of our specimens are sent fresh because surgery says formalin cannot be in the operating rooms. I'm told it comes from AORN, which is the association for operating room nurses. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Monday, August 20, 2012 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Operating Rooms Hi All, I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 16 Date: Tue, 21 Aug 2012 07:33:39 -0500 From: Glen Dawson Subject: [Histonet] Renal Cell Carcinoma Poll To: histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, What is your favorite Renal Cell Carcinoma Antibody? I'm using the Leica Bond stainer with Leica's detection & am looking for the best fit. In other words, the antibody that I am using is working, but I want it to look better. Any responses would be greatly appreciated. Glen Dawson BS, HT(ASCP), QIHC IHC Technical Specialist Janesville, WI ------------------------------ Message: 17 Date: Tue, 21 Aug 2012 07:05:23 -0600 From: "O'Donnell, Bill" Subject: RE: [Histonet] Formalin and Operating Rooms To: "Paula Sicurello" , "Debra Siena" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 18 Date: Tue, 21 Aug 2012 13:35:56 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Formalin and Operating Rooms To: "O'Donnell, Bill" , Paula Sicurello , Debra Siena Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D50E@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" I also have not heard of any recent rule changes. Being aware of the formalin mishap in OR, our hospital has been using for over 20 years, a light blue tinged 10%NBF that the supplier makes up special. The blue is actually just a few drops of food coloring that the manufacturer adds, and the blue does NOT carry over through processing and staining. I am also aware that we are not the only hospital using this blue formalin. There is no confusion for the OR personnel when they grab the appropriate container (NBF v/s saline). hope this helps, Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of O'Donnell, Bill [billodonnell@catholichealth.net] Sent: Tuesday, August 21, 2012 9:05 AM To: Paula Sicurello; Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Operating Rooms I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 21 Aug 2012 09:02:17 -0500 From: Subject: RE: [Histonet] DAB precipitate on H. pylori To: , , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DF28025AD@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" We never had a problem until they changed AB's on us. We sent them slides procedures etc to test but in the mean time we tried Cell Marques AB and it was clean. Problem solved. Now we all need to demand that Ventana switch back to Cell Marques H pylori so that we do not have to pay for those #@*&% prep kits. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, August 20, 2012 2:11 PM To: Dorothy Glass; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB precipitate on H. pylori Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Monday, August 20, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB precipitate on H. pylori I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 21 Aug 2012 14:40:37 +0000 From: "Houston, Ronald" Subject: [Histonet] Position - Snr Histotechnician To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have a vacancy for a Senior Histotechnican. The Senior Histotechnician is responsible for performing a wide variety of histotechnological testing including, but not limited to, microtomy, cryotomy, complex special stains, IF, IHC, enzyme histochemistry and electron microscopy. The potential exists to become involved in research projects within the department. A working knowledge of Lean principles is useful. Theoretical knowledge as well as high quality practical ability is required. Candidates with expertise in Immunohistochemistry and/or Electron Microscopy preferred. Experience in pediatrics desirable. Registered HT(ASCP) or equivalent, with a minimum of 5 years histopathology experience in a similar laboratory Please apply on our web-site, and/or contact Sue Doud (614-355-41-5) or myself for more details Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. 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Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 25 ***************************************** ________________________________ This e-mail, including any attachments, is a confidential business communication, and may contain information that is confidential, proprietary and/or privileged. This e-mail is intended only for the individual(s) to whom it is addressed, and may not be saved, copied, printed, disclosed or used by anyone else. If you are not the(an) intended recipient, please immediately delete this e-mail from your computer system and notify the sender. Thank you. From LPaveli1 <@t> hurleymc.com Wed Aug 22 08:58:14 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Aug 22 08:58:28 2012 Subject: [Histonet] RE: Leica Bond Stainer In-Reply-To: <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> References: <6751b716-a35f-48fa-ac73-ec6b4514b511@exch-cas01prd.corp.mpi.com>, <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D627@EXCHANGEMB1.hmc.hurleymc.com> We have had the Leica Bond Max for ~3 yrs. The software is easy to learn and, in my opinion, it is a nice combination between the Dako Autostainer (it's openness), and nice heating features the Ventana Benchmark offers. We have no problems using other companies antibodies. After starting a run, it is really nice to have the ability to add another run. Would strongly recommend a yearly PM and daily (DAILY) cleaning at the end of each day. If this machine gets dirty, it will affect the quality of your staining. Even if it LOOKS clean, it only takes 5 minutes each day to maintain a clean machine for reliable consistent results. This was the biggest lesson we learned about the Leica. (bring an old toothbrush from home!) Cons? We have purchased a Ventana, Dako, and now Leica. Just remember that they are all machines. They all will have, from time to time, staining issues for no apparent reason, and thus will need to be repeated. All of them. That being said, we all are very happy with the Leica. And you know if the docs are happy.............! Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Crowell, Thomas [Thomas.Crowell@MPI.com] Sent: Wednesday, August 22, 2012 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Stainer Hello all, Our laboratory is considering a purchase, and Leica will be giving a presentation on the unit in September. I was hoping to get some responses of pros/cons/quirks from users to help in our assessment. We are currently using Ventana XT to look at PD biomarkers in cell lines, xenografts and some clinical samples. I would be interested in a comparison of performance and reliability between these platforms. Any responses would be greatly appreciated. Thanks! Tom Thomas Crowell Supplemental Contractor Molecular Pathology Millennium: The Takeda Oncology Company 35 Landsdowne Street | Cambridge, MA | 02139 Phone: 617.444.2303 Fax: 617.551.3744 thomas.crowell@mpi.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, August 21, 2012 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Productivity (Heckford, Karen - SMMC-SF) 2. Ventana Symphony reagents (Clare Thornton) 3. DAB precipitate on H. pylori (Dorothy Glass) 4. RE: DAB precipitate on H. pylori (Vanessa Perez) 5. RE: Productivity (CHRISTIE GOWAN) 6. Re: Productivity (Rene J Buesa) 7. Re: Histonet Digest, Vol 105, Issue 24 (Me) 8. New Position (Wilson A) 9. Formalin and Operating Rooms (Debra Siena) 10. Re: Formalin and Operating Rooms (Mark Tarango) 11. Re: Formalin and Operating Rooms (Paula Sicurello) 12. annoying crystals on sections (E. Wayne Johnson) 13. help ! Dako Seymour labeling software - autostainer plus (richard wild) 14. RE: Productivity (Boyd, Debbie M) 15. RE: Formalin and Operating Rooms (Horn, Hazel V) 16. Renal Cell Carcinoma Poll (Glen Dawson) 17. RE: Formalin and Operating Rooms (O'Donnell, Bill) 18. RE: Formalin and Operating Rooms (Lynette Pavelich) 19. RE: DAB precipitate on H. pylori (Susan.Walzer@HCAHealthcare.com) 20. Position - Snr Histotechnician (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Aug 2012 10:42:39 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Productivity To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 2 Date: Mon, 20 Aug 2012 13:49:25 -0400 From: Clare Thornton Subject: [Histonet] Ventana Symphony reagents To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have some Symphony reagents and coverslips that we no longer need. If you are in the New England area, we could deliver them with our courier service. If you are outside of New England, if you pay the shipping we will send them. Please email me off list if you would like to know what we have and are interested in taking any/all of it. Have a great day everyone! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 3 Date: Mon, 20 Aug 2012 11:12:50 -0700 (PDT) From: Dorothy Glass Subject: [Histonet] DAB precipitate on H. pylori To: histonet@lists.utsouthwestern.edu Message-ID: <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? ------------------------------ Message: 4 Date: Mon, 20 Aug 2012 13:10:40 -0500 From: Vanessa Perez Subject: RE: [Histonet] DAB precipitate on H. pylori To: Dorothy Glass , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Monday, August 20, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB precipitate on H. pylori I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 20 Aug 2012 18:37:55 +0000 From: CHRISTIE GOWAN Subject: RE: [Histonet] Productivity To: , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Karen, The best way to communicate with management is through data. If you have access to say the last 3 years of blocks and or slides cut and you compare it to accessions or cpt codes then you have a roadmap of where you have been in the past and where you are now. Do you have more or less employees and is this a temporary lull in numbers or has this dip occurred before. At least you will be able to formulate a more informed response to their request for cutting back hours. This is always a complicated issue and we have seen an increase in case complexity as well in our institution so our numbers of blocks vs. specimen count is skewed as well. Tell them you will cut hours but you will need all new equipment to continue to meet your deadlines! Ha!. Let us know how it turns out and good luck! Christie Gowan, HT (ASCP) UAB Hospital Histology Supervisor Surgical Pathology 205 934 4991 cgowan@uabmc.edu > From: Karen.Heckford@DignityHealth.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 20 Aug 2012 10:42:39 -0700 > Subject: [Histonet] Productivity > > Good Morning, > I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 20 Aug 2012 14:03:33 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Productivity To: "Heckford, Karen - SMMC-SF" , "histonet@lists.utsouthwestern.edu" Message-ID: <1345496613.86112.YahooMailNeo@web121401.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Karen: "Productivity" and its measures depends on who is going to use it. 1- For the pathologists as a whole they are interested in CASES they receive and sign either on a daily or monthly basis; 2- For management it is measured in how many cases the pathologists can sign and therefore the administration can bill/collect 3- as the Lead Tech you are interested is the work output of your staff specially how many blocks each can section either per hour or per shift or how many minutes it takes to complete a FS. Mind that the ONLY productivity rate defined by CAP is the time a FS can be diagnosed from the moment it is received at histology and that has to be 20 minutes or less. Nowadays almost every lab has an automated stainer so the number of slides has been "downgraded" as a productivity index, although it is not the same to?prepare 1 than 20 slides. The unit of time is also a subject of debate: some prefer the "hour" but from the whole lab the index most desired is the number of CASES a lab can complete every day. Under separate cover I am sending you some articles I wrote on the subject so you can select those productivity indices most fit your lab. Ren? J. ________________________________ From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, August 20, 2012 1:42 PM Subject: [Histonet] Productivity Good Morning, I know we have been over this before, how does your lab measure productivity?? Are you going by block and slide or by CPT code?? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value.? As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides.? I do everything from GI specimens to big breast cases.? They will tell me that our productivity is down and we need to cut hours.? I just do not know exactly how to drill it in to them that things are not always as they appear on paper.? I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 20 Aug 2012 17:38:44 -0400 From: Me Subject: [Histonet] Re: Histonet Digest, Vol 105, Issue 24 To: histonet@lists.utsouthwestern.edu Message-ID: <1db21a96-209c-44f0-a517-da793748aa2c@blur> Content-Type: text/plain; Format="Flowed"; DelSp="Yes"; charset="US-ASCII" I have a question for all in Histoland... Concerning the CAP revision on the use of negative controls...I work in a NJ lab that does work for NY offices, CAP states we no longer need to run controls if using biotin free detection but does NY state board of health agree? Not running those extra slides is a huge money and time saver. We are using Leica Bond III and the polymer detection. Anyone have an answer or know where I can get the answer? Jen Elz Pathology Solutions Eatontown, NJ Sent from my Verizon Wireless Droid -----Original message----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mon, Aug 20, 2012 17:00:30 GMT+00:00 Subject: Histonet Digest, Vol 105, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: disposal of formalin (MaryK Mendell) 2. RE: Microtome feedback (Bea DeBrosse-Serra) 3. RE: Microtome feedback (Lynette Pavelich) 4. Immuno Tech (Carol Fields) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Aug 2012 07:29:45 -0400 From: MaryK Mendell Subject: [Histonet] RE: disposal of formalin To: "Lake,Debbie" , "histonet@lists.utsouthwestern.edu" Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D33D@EXMBX01.mmeprod.cbeyond> Content-Type: text/plain; charset="us-ascii" We also use a company to pick it up along with xylene & alcohol And you are responsible from craddle to grave. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie [Debbie.Lake@mgh.net] Sent: Friday, August 17, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 20 Aug 2012 06:55:39 -0700 From: Bea DeBrosse-Serra Subject: RE: [Histonet] Microtome feedback To: "'Ian R Bernard'" , Fred Underwood , Histonet Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34B8C@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" It is a high quality work horse. I've worked with newer and older microtomes of this very model and never had any problems. You can utilize the motorized function to minimize repetitive motion, but you can also use it manually if needed. What is there not to like? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: Ian R Bernard [mailto:ibernard@uab.edu] Sent: Saturday, August 18, 2012 3:53 AM To: Bea DeBrosse-Serra; Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback What is great about the Leica RM2255? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Friday, August 17, 2012 8:58 PM To: Fred Underwood; Histonet Subject: RE: [Histonet] Microtome feedback Fred, The Leica RM2255 all the way! Go Dayton! Bea ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 20 Aug 2012 14:27:35 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Microtome feedback To: Fred Underwood , Histonet Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D3F4@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" We purchased the Leica RM2255 just over a year ago. It is the only one we have and is primarily used by one tech. In her absence, others use her machine. What is great about it, is that it is VERY easy to adapt to it. The controls are easy to figure out, cuts beautiful slides, and can be used either manually or completely automatic. Additionally, it has a "homing" option for the block holder.........pretty handy if you need to adjust the block holder often! In the future, I plan on replacing 2 more microtomes with this Leica microtome. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Fred Underwood [funderwood@mcohio.org] Sent: Friday, August 17, 2012 3:48 PM To: Histonet Subject: [Histonet] Microtome feedback Hello and TGIF to everyone. I'm exploring getting a new microtome and would like to draw on the vast knowledge and experience out there in Histoland. The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+, and the Leica RM2255. Thanks, Fred Underwood Montgomery County Coroner's Office Dayton, OH ------------------------------ Message: 4 Date: Mon, 20 Aug 2012 12:20:37 -0400 From: "Carol Fields" Subject: [Histonet] Immuno Tech To: Cc: Message-ID: <731941C266951A47BEF11E5EFAAED9C911C4059C@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" If anyone has an Immuno Job Description you are willing to share I would appreciate it. My email address is carol.fields@northside.com. Thanks to all. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 24 ***************************************** ------------------------------ Message: 8 Date: Mon, 20 Aug 2012 15:23:06 -0700 (PDT) From: Wilson A Subject: [Histonet] New Position To: "histonet@lists.utsouthwestern.edu" Message-ID: <1345501386.59218.YahooMailNeo@web125406.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? ? Hi, ??? I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY. Willing to relocate. Thanks, Wilson. ------------------------------ Message: 9 Date: Mon, 20 Aug 2012 17:25:06 -0500 From: Debra Siena Subject: [Histonet] Formalin and Operating Rooms To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com ------------------------------ Message: 10 Date: Mon, 20 Aug 2012 16:04:16 -0700 From: Mark Tarango Subject: Re: [Histonet] Formalin and Operating Rooms To: Debra Siena Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Debra, Formaldehyde was listed as "known to be a human carcinogen" in the 12th Report on Carcinogens (2011) put out by the Department of Health and Human Services. Here is a link http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf This is what is probably behind any recent changes to its use or availability. Johnson & Johnson just a few days ago announced that they're going to take formaldehyde out of baby shampoo! Yah! Mark On Mon, Aug 20, 2012 at 3:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Mon, 20 Aug 2012 19:04:33 -0400 From: Paula Sicurello Subject: Re: [Histonet] Formalin and Operating Rooms To: Debra Siena Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in the > room so that they can place formalin onto the sample right away? I was > wondering if anyone has heard of this, if you could tell me more about > where it is coming from so that I can access a copy of it. We have had > some inquiries and I have not heard of this. I appreciate any help that > you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Tue, 21 Aug 2012 07:20:06 +0800 From: "E. Wayne Johnson" Subject: [Histonet] annoying crystals on sections To: histonet@lists.utsouthwestern.edu Message-ID: <5032C626.5040206@pigsqq.org> Content-Type: text/plain; charset=ISO-8859-1; format=flowed We are having problems with crystals precipitated on our slides which are H&E stains on tissues from pigs. Tissues are fixed in buffered formalin. We had trouble months ago with formalin pigment and we had resolved that by using ammonia in EtOH or picric acid in EtOH. Sometimes we receive fixed samples from the field that are not buffered but presently all of our tissues are fixed in neutral phosphate buffered formalin. We moved the Sakura autostainer to a different location under a fume hood on a different floor of the building to get the solvent odor out of our work area. Immediately we began to see a tremendous degradation in slide quality due to what we initially thought was formalin pigment. We have changed all of the solutions and all of the stains. We find that if we use Milli-q water instead of tap water for rinsing (done by hand in that case) we dont see the crystals, but the eosin staining quality is not acceptable after rinsing in the acidic (ph ~5) Milli-Q water. Our tap water is neutral to slightly alkaline and is very hard with calcium. We do all sorts of tissues for diagnosis of pig diseases. Sometimes the slides are quite acceptable but sometimes particularly when looking at small intestine, the crystals are very annoying. The crystals occur randomly on the slide except that there is a tendency for them to be centered on nuclei particularly in intestinal epithelium. The crystals are birefringent in polarized light but seem to be generally clear not dark like the formalin pigment we had seen before. Neither ammonia nor picric acid remove these, and now if we use alcoholic ammonia to treat the slides, the slides come out too blue. Our slides are cut at 4 to 5 microns. Brain has the least problem, small intestine seems worst. We have gone back and cut some blocks that previously stained beautifully with no pigment or precipitate problems and those slides also now have the same problem, either crystals if washed with tap water, or poor eosin staining if rinsed with MilliQ water. Our next step is to examine the slides microscopically at every step and try to find at which step the problem is occurring. Any thoughts or similar experiences? E. Wayne Johnson, DVM Enruikang AgTech MOA Feed Industry Centre Beijing ------------------------------ Message: 13 Date: Tue, 21 Aug 2012 08:48:15 +0200 From: richard wild Subject: [Histonet] help ! Dako Seymour labeling software - autostainer plus To: histonet@lists.utsouthwestern.edu Message-ID: <50332F2F.8070508@wanadoo.fr> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello dear histoneters First let me thank you all for your help for lost password (autostainer plus software) - "a" - "a" tip is ok. I am begining to test the autostainer and now eveything seems fine (there was a stuck tube but I could settle the problem) _Corrupted files : Dako Seymour software (TLP 2742 printer)_ Autostainer plus can print labels directly from main software. But printing labels can be done from another computer if you use a another provided software (Dako Seymour labeling software) My software dako Seymour 5.0 is on two floppies and two compressed files are corrupted */VB40016.DL_/* (on first floppy) and */ARW01DN0.IC_/* (on second floppy) _It would be great if someone could send me by mail the two missing working files_ (compressed format, exactly as I have written them here would be better, but may be uncompressed files that are in the computer could work - these files are not very heavy and can easily be sent) I asked Dako France but they dont have anymore the floppies of the Dako Seymour labeling software (5.0) _Does someone have a list or a link of best protocols (procedures) for the machine ?_ have a nice day Best regards R Wild ------------------------------ Message: 14 Date: Tue, 21 Aug 2012 11:57:13 +0000 From: "Boyd, Debbie M" Subject: [Histonet] RE: Productivity To: "Heckford, Karen - SMMC-SF" , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB7624639D8D03C@TN001WEXMBX12.US.chs.net> Content-Type: text/plain; charset="us-ascii" Our unit of measure is billable test. But, my director adds in extra (not sure how) for manual vs. automation in the clinical lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, August 20, 2012 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Productivity Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 15 Date: Tue, 21 Aug 2012 07:15:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] RE: Formalin and Operating Rooms To: "'Debra Siena'" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A17CC@EVS1.archildrens.org> Content-Type: text/plain; charset="iso-8859-1" Debra and others: All of our specimens are sent fresh because surgery says formalin cannot be in the operating rooms. I'm told it comes from AORN, which is the association for operating room nurses. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Monday, August 20, 2012 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Operating Rooms Hi All, I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 16 Date: Tue, 21 Aug 2012 07:33:39 -0500 From: Glen Dawson Subject: [Histonet] Renal Cell Carcinoma Poll To: histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, What is your favorite Renal Cell Carcinoma Antibody? I'm using the Leica Bond stainer with Leica's detection & am looking for the best fit. In other words, the antibody that I am using is working, but I want it to look better. Any responses would be greatly appreciated. Glen Dawson BS, HT(ASCP), QIHC IHC Technical Specialist Janesville, WI ------------------------------ Message: 17 Date: Tue, 21 Aug 2012 07:05:23 -0600 From: "O'Donnell, Bill" Subject: RE: [Histonet] Formalin and Operating Rooms To: "Paula Sicurello" , "Debra Siena" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395713E23@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 18 Date: Tue, 21 Aug 2012 13:35:56 +0000 From: Lynette Pavelich Subject: RE: [Histonet] Formalin and Operating Rooms To: "O'Donnell, Bill" , Paula Sicurello , Debra Siena Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D50E@EXCHANGEMB1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" I also have not heard of any recent rule changes. Being aware of the formalin mishap in OR, our hospital has been using for over 20 years, a light blue tinged 10%NBF that the supplier makes up special. The blue is actually just a few drops of food coloring that the manufacturer adds, and the blue does NOT carry over through processing and staining. I am also aware that we are not the only hospital using this blue formalin. There is no confusion for the OR personnel when they grab the appropriate container (NBF v/s saline). hope this helps, Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of O'Donnell, Bill [billodonnell@catholichealth.net] Sent: Tuesday, August 21, 2012 9:05 AM To: Paula Sicurello; Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin and Operating Rooms I know of no rules, though there may well be. I do know that several years ago a patient was injected (IV)with formalin in the OR. Results were NOT good. I know that the OR in the place I was working at the time would no longer allow for formalin in the OR suite. The tissues were put in formalin in a separate area - by OR staff. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Monday, August 20, 2012 6:05 PM To: Debra Siena Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin and Operating Rooms I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena wrote: > Hi All, > > I would like to ask if anyone has heard of any new regulations or laws > that state that the Operating Room can't have formalin available in > the room so that they can place formalin onto the sample right away? > I was wondering if anyone has heard of this, if you could tell me more > about where it is coming from so that I can access a copy of it. We > have had some inquiries and I have not heard of this. I appreciate > any help that you can give and sorry, that I don't have more information. Best wishes. > > Debbie Siena, HT(ASCP)QIHC > StatLab Medical Products > Technical Support Manager > 407 Interchange Street | McKinney, TX 75071 > t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | > www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 21 Aug 2012 09:02:17 -0500 From: Subject: RE: [Histonet] DAB precipitate on H. pylori To: , , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DF28025AD@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" We never had a problem until they changed AB's on us. We sent them slides procedures etc to test but in the mean time we tried Cell Marques AB and it was clean. Problem solved. Now we all need to demand that Ventana switch back to Cell Marques H pylori so that we do not have to pay for those #@*&% prep kits. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, August 20, 2012 2:11 PM To: Dorothy Glass; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB precipitate on H. pylori Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Monday, August 20, 2012 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB precipitate on H. pylori I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 21 Aug 2012 14:40:37 +0000 From: "Houston, Ronald" Subject: [Histonet] Position - Snr Histotechnician To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have a vacancy for a Senior Histotechnican. The Senior Histotechnician is responsible for performing a wide variety of histotechnological testing including, but not limited to, microtomy, cryotomy, complex special stains, IF, IHC, enzyme histochemistry and electron microscopy. The potential exists to become involved in research projects within the department. A working knowledge of Lean principles is useful. Theoretical knowledge as well as high quality practical ability is required. Candidates with expertise in Immunohistochemistry and/or Electron Microscopy preferred. Experience in pediatrics desirable. Registered HT(ASCP) or equivalent, with a minimum of 5 years histopathology experience in a similar laboratory Please apply on our web-site, and/or contact Sue Doud (614-355-41-5) or myself for more details Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 25 ***************************************** ________________________________ This e-mail, including any attachments, is a confidential business communication, and may contain information that is confidential, proprietary and/or privileged. This e-mail is intended only for the individual(s) to whom it is addressed, and may not be saved, copied, printed, disclosed or used by anyone else. If you are not the(an) intended recipient, please immediately delete this e-mail from your computer system and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Wed Aug 22 09:09:40 2012 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Wed Aug 22 09:09:52 2012 Subject: [Histonet] Leica Bond Stainer In-Reply-To: <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> References: <6751b716-a35f-48fa-ac73-ec6b4514b511@exch-cas01prd.corp.mpi.com> <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> Message-ID: <34684A96-F24E-493B-9745-C4BD1B640143@yahoo.com> The Bond III and Bond max are great. The Bond III has a shorter run time. Not to mention the cost savings on both machines. And yes, maintenance is a piece of cake. Good luck! Sent from the iPhone of Kim Tournear ?? On Aug 22, 2012, at 8:13 AM, "Crowell, Thomas" wrote: > Hello all, > > Our laboratory is considering a purchase, and Leica will be giving a presentation on the unit in September. I was hoping to get some responses of pros/cons/quirks from users to help in our assessment. We are currently using Ventana XT to look at PD biomarkers in cell lines, xenografts and some clinical samples. I would be interested in a comparison of performance and reliability between these platforms. > > Any responses would be greatly appreciated. > Thanks! > Tom > > Thomas Crowell > Supplemental Contractor > Molecular Pathology > > Millennium: The Takeda Oncology Company > 35 Landsdowne Street | Cambridge, MA | 02139 > Phone: 617.444.2303 Fax: 617.551.3744 > thomas.crowell@mpi.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu > Sent: Tuesday, August 21, 2012 10:44 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 105, Issue 25 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Productivity (Heckford, Karen - SMMC-SF) > 2. Ventana Symphony reagents (Clare Thornton) > 3. DAB precipitate on H. pylori (Dorothy Glass) > 4. RE: DAB precipitate on H. pylori (Vanessa Perez) > 5. RE: Productivity (CHRISTIE GOWAN) > 6. Re: Productivity (Rene J Buesa) > 7. Re: Histonet Digest, Vol 105, Issue 24 (Me) > 8. New Position (Wilson A) > 9. Formalin and Operating Rooms (Debra Siena) > 10. Re: Formalin and Operating Rooms (Mark Tarango) > 11. Re: Formalin and Operating Rooms (Paula Sicurello) > 12. annoying crystals on sections (E. Wayne Johnson) > 13. help ! Dako Seymour labeling software - autostainer plus > (richard wild) > 14. RE: Productivity (Boyd, Debbie M) > 15. RE: Formalin and Operating Rooms (Horn, Hazel V) > 16. Renal Cell Carcinoma Poll (Glen Dawson) > 17. RE: Formalin and Operating Rooms (O'Donnell, Bill) > 18. RE: Formalin and Operating Rooms (Lynette Pavelich) > 19. RE: DAB precipitate on H. pylori (Susan.Walzer@HCAHealthcare.com) > 20. Position - Snr Histotechnician (Houston, Ronald) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 20 Aug 2012 10:42:39 -0700 > From: "Heckford, Karen - SMMC-SF" > Subject: [Histonet] Productivity > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 20 Aug 2012 13:49:25 -0400 > From: Clare Thornton > Subject: [Histonet] Ventana Symphony reagents > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We have some Symphony reagents and coverslips that we no longer need. If you are in the New England area, we could deliver them with our courier service. If you are outside of New England, if you pay the shipping we will send them. Please email me off list if you would like to know what we have and are interested in taking any/all of it. > > Have a great day everyone! > > Clare > > Clare J. Thornton, HTL(ASCP), QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > > > ------------------------------ > > Message: 3 > Date: Mon, 20 Aug 2012 11:12:50 -0700 (PDT) > From: Dorothy Glass > Subject: [Histonet] DAB precipitate on H. pylori > To: histonet@lists.utsouthwestern.edu > Message-ID: > <1345486370.81806.YahooMailClassic@web114516.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? > > ------------------------------ > > Message: 4 > Date: Mon, 20 Aug 2012 13:10:40 -0500 > From: Vanessa Perez > Subject: RE: [Histonet] DAB precipitate on H. pylori > To: Dorothy Glass , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support??? > > Vanessa Perez Garcia > Histology Supervisor > Pathology Reference Lab > 210-892-3746 > 210-892-3732 > vperez@pathreflab.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass > Sent: Monday, August 20, 2012 1:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] DAB precipitate on H. pylori > > I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Mon, 20 Aug 2012 18:37:55 +0000 > From: CHRISTIE GOWAN > Subject: RE: [Histonet] Productivity > To: , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Karen, > The best way to communicate with management is through data. If you have access to say the last 3 years of blocks and or slides cut and you compare it to accessions or cpt codes then you have a roadmap of where you have been in the past and where you are now. Do you have more or less employees and is this a temporary lull in numbers or has this dip occurred before. At least you will be able to formulate a more informed response to their request for cutting back hours. This is always a complicated issue and we have seen an increase in case complexity as well in our institution so our numbers of blocks vs. specimen count is skewed as well. Tell them you will cut hours but you will need all new equipment to continue to meet your deadlines! Ha!. Let us know how it turns out and good luck! > > Christie Gowan, HT (ASCP) > UAB Hospital > Histology Supervisor > Surgical Pathology > 205 934 4991 > cgowan@uabmc.edu > > >> From: Karen.Heckford@DignityHealth.org >> To: histonet@lists.utsouthwestern.edu >> Date: Mon, 20 Aug 2012 10:42:39 -0700 >> Subject: [Histonet] Productivity >> >> Good Morning, >> I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!! >> >> >> Karen Heckford HT ASCP CE >> Lead Histology Technician >> St. Mary's Medical Center >> 450 Stanyan St. >> San Francisco, Ca. 94117 >> 415-668-1000 ext. 6167 >> karen.heckford@dignityhealth.org >> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 6 > Date: Mon, 20 Aug 2012 14:03:33 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Productivity > To: "Heckford, Karen - SMMC-SF" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1345496613.86112.YahooMailNeo@web121401.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Karen: > "Productivity" and its measures depends on who is going to use it. > 1- For the pathologists as a whole they are interested in CASES they receive and sign either on a daily or monthly basis; > 2- For management it is measured in how many cases the pathologists can sign and therefore the administration can bill/collect > 3- as the Lead Tech you are interested is the work output of your staff specially how many blocks each can section either per hour or per shift or how many minutes it takes to complete a FS. Mind that the ONLY productivity rate defined by CAP is the time a FS can be diagnosed from the moment it is received at histology and that has to be 20 minutes or less. > Nowadays almost every lab has an automated stainer so the number of slides has been "downgraded" as a productivity index, although it is not the same to?prepare 1 than 20 slides. > The unit of time is also a subject of debate: some prefer the "hour" but from the whole lab the index most desired is the number of CASES a lab can complete every day. > Under separate cover I am sending you some articles I wrote on the subject so you can select those productivity indices most fit your lab. > Ren? J. > > > ________________________________ > From: "Heckford, Karen - SMMC-SF" > To: "histonet@lists.utsouthwestern.edu" > Sent: Monday, August 20, 2012 1:42 PM > Subject: [Histonet] Productivity > > Good Morning, > I know we have been over this before, how does your lab measure productivity?? Are you going by block and slide or by CPT code?? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value.? As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides.? I do everything from GI specimens to big breast cases.? They will tell me that our productivity is down and we need to cut hours.? I just do not know exactly how to drill it in to them that things are not always as they appear on paper.? I am talking in circles until I am blue in the face...AUGH!!!!!! > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Mon, 20 Aug 2012 17:38:44 -0400 > From: Me > Subject: [Histonet] Re: Histonet Digest, Vol 105, Issue 24 > To: histonet@lists.utsouthwestern.edu > Message-ID: <1db21a96-209c-44f0-a517-da793748aa2c@blur> > Content-Type: text/plain; Format="Flowed"; DelSp="Yes"; > charset="US-ASCII" > > I have a question for all in Histoland... > Concerning the CAP revision on the use of negative controls...I work in a NJ > lab that does work for NY offices, CAP states we no longer need to run > controls if using biotin free detection but does NY state board of health > agree? Not running those extra slides is a huge money and time saver. We are > using Leica Bond III and the polymer detection. Anyone have an answer or > know where I can get the answer? > > Jen Elz > Pathology Solutions > Eatontown, NJ > Sent from From BDeBrosse-Serra <@t> isisph.com Wed Aug 22 10:01:30 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Aug 22 10:01:49 2012 Subject: [Histonet] RE: Leica Bond Stainer In-Reply-To: <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> References: <6751b716-a35f-48fa-ac73-ec6b4514b511@exch-cas01prd.corp.mpi.com> <7D6672082FDD264F81CB7F124CFF65BB64F0@EX10-MBX01PRD.corp.mpi.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34B98@EXCHMB01.isis.local> BondMax and Bond III are great. Very intuitive, open and fast. And you can deparaffinize and retrieve online, which is a big plus in my mind. But then again, you don't have to, but you can. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Crowell, Thomas Sent: Wednesday, August 22, 2012 6:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Stainer Hello all, Our laboratory is considering a purchase, and Leica will be giving a presentation on the unit in September. I was hoping to get some responses of pros/cons/quirks from users to help in our assessment. We are currently using Ventana XT to look at PD biomarkers in cell lines, xenografts and some clinical samples. I would be interested in a comparison of performance and reliability between these platforms. Any responses would be greatly appreciated. Thanks! Tom Thomas Crowell Supplemental Contractor Molecular Pathology Millennium: The Takeda Oncology Company 35 Landsdowne Street | Cambridge, MA | 02139 Phone: 617.444.2303 Fax: 617.551.3744 thomas.crowell@mpi.com From Mark.Elliott <@t> hli.ubc.ca Wed Aug 22 13:20:02 2012 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Aug 22 13:19:42 2012 Subject: [Histonet] Versican antibody for dog tissue In-Reply-To: <9E0A26B4.920@mail.mrl.ubc.ca> References: <9E0A26B4.920@mail.mrl.ubc.ca> Message-ID: <5034C062020000D6000506CC@mail.mrl.ubc.ca> Can anyone recommend a versican antibody that works on dog tissue? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Elizabeth.Cameron <@t> jax.org Wed Aug 22 13:48:43 2012 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Aug 22 13:48:52 2012 Subject: [Histonet] CD20 for Mouse Tissue Message-ID: Does anyone know of a CD20 antibody that works well on paraffin embedded mouse tissue? Thanks! -Liz The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Margaret.Perry <@t> sdstate.edu Wed Aug 22 13:48:00 2012 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Aug 22 13:49:21 2012 Subject: [Histonet] gloves Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE041860@SDSU-EX03.jacks.local> Where do you buy the Sempermed gloves? Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From eca9 <@t> georgetown.edu Wed Aug 22 14:25:13 2012 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Wed Aug 22 14:25:43 2012 Subject: [Histonet] CD20 for Mouse Tissue In-Reply-To: References: Message-ID: Hi Liz, We just used CD20 cat.no. 14-0201 from eBioscience on mouse livers and spleen. It worked great. Email me separate if you would like to send you the protocol. Eva Eva Permaul Georgetown University On Wed, Aug 22, 2012 at 2:48 PM, Elizabeth Cameron < Elizabeth.Cameron@jax.org> wrote: > Does anyone know of a CD20 antibody that works well on paraffin embedded > mouse tissue? > Thanks! > -Liz > > > The information in this email, including attachments, may be confidential > and is intended solely for the addressee(s). If you believe you received > this email by mistake, please notify the sender by return email as soon as > possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Milton.Gomez <@t> nyumc.org Wed Aug 22 18:15:47 2012 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Wed Aug 22 18:15:59 2012 Subject: [Histonet] HT study material Message-ID: <7A7F4F00BF2D2D46A158AE61E0D2B6302A1F1C48@MSGWCDCPMB27.nyumc.org> Dear Histonetters, Does anyone have study material that would like to either sell and or share for the HT (ASCP) examination? Thank you very much in advance, MG From JMacDonald <@t> mtsac.edu Thu Aug 23 00:31:21 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Aug 23 00:31:27 2012 Subject: [Histonet] CJD processing Message-ID: Does anyone know of a lab in Southern California that is equipped to handle CJD (suspected) cases? Thank you, Jennifer From sforeman <@t> labpath.com Thu Aug 23 06:51:05 2012 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Aug 23 06:52:14 2012 Subject: [Histonet] Procollagen Message-ID: <000301cd8125$93825e20$ba871a60$@com> One of our pathologists is looking to send a case out for Procollagen IHC staining. Do you know who is offering this testing? Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 From kcastillo <@t> frii.com Thu Aug 23 09:29:17 2012 From: kcastillo <@t> frii.com (kcastillo@frii.com) Date: Thu Aug 23 09:29:24 2012 Subject: [Histonet] nails staying on slides Message-ID: <9ccaa6bf0d6385dd63fa07dce03d8778@frii.com> Hi Everyone in Histoland, Having problems keeping my nails on plus slides when staining. They of course fall off during the PAS staining process. Can anyone help me please, thank you! Kristy From LPaveli1 <@t> hurleymc.com Thu Aug 23 12:27:23 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Aug 23 12:27:30 2012 Subject: [Histonet] RE: HT study material In-Reply-To: <7A7F4F00BF2D2D46A158AE61E0D2B6302A1F1C48@MSGWCDCPMB27.nyumc.org> References: <7A7F4F00BF2D2D46A158AE61E0D2B6302A1F1C48@MSGWCDCPMB27.nyumc.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF55D744@EXCHANGEMB1.hmc.hurleymc.com> I would try the NSH website www.nsh.org, as they have educational materials available (answers in the back) and also the Michigan Society (MSH) website www.mihisto.org and to the 'estore' tab to view their educational materials. Be advised that these are study workbooks and do not have the answers in the back. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gomez, Milton [Milton.Gomez@nyumc.org] Sent: Wednesday, August 22, 2012 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT study material Dear Histonetters, Does anyone have study material that would like to either sell and or share for the HT (ASCP) examination? Thank you very much in advance, MG _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Thu Aug 23 12:27:30 2012 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Thu Aug 23 12:27:42 2012 Subject: [Histonet] Cx'ing pathology orders in computer based system Message-ID: <938D716CD445614ABBB817517557B6F407756D331F@NADCWPMSGCMS09.hca.corpad.net> Does anyone know if there is a written reference (CMS, JCAHO or CAP) as to who is allowed to cancel or modify a pathology order due to misidentification of specimen site? Scenario- Pathology LIS receives electronic order-Order being "pathology or Cytology to lab", but there is specific specimen identification of Large Intestine (specimen labeled as Large Intestine), pathologist opens container and says no this is Small Intestine, does pathology have the "right" to modify what was entered into the LIS? Or to even re-label the specimen correctly? (before you Squawk-I know that you make the nurse re-do everything CYA) but wanted to know if there was any documentation that says the "Nurse" has to do it and that the functionality should not be left in the hands of the pathology department to change the order. Thanks Jessica Vacca Epic Anatomic Pathology Application Lead HCA Clinical Services Group 2545 Park Plaza Nashville, TN 32703 t: 615-579-0121 o: 615-344-5370 e: Jessica.Vacca@hcahealthcare.com From m.kap.1 <@t> erasmusmc.nl Thu Aug 23 13:08:21 2012 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Thu Aug 23 13:08:28 2012 Subject: [Histonet] SPIDIA Prostate tumor ring trial Message-ID: Dear all, On behalf of the SPIDIA consortium (www.spidia.eu), I would like to invite urology/prostate pathologists in your network to participate in the SPIDIA Prostate Tumor Ring Trial. The ring trial consists of the evaluation of 15 digital H&E slides, this will take approximately 20 minutes of your time. All participating pathologists will be co-authors of the forthcoming paper. Would you please be as kind to forward this mail to your colleagues. For statistical reasons, I would like you to invite at least 30 pathologists. You can find more information in the attached document. Please note that the ring trial will start in the second week of September. Further information will be sent to the individual participants. Could you please let me know if you and/or your colleagues are interested? Thank you very much for you cooperation, I will be awaiting your reply. Best regards, Marcel Kap, BSc PhD Candidate Erasmus MC Tissuebank and Tissue Research Support Unit Department of Pathology Josephine Nefkens Institute Erasmus MC Rotterdam > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." > Today's Topics: > 1. Versican antibody for dog tissue (Mark Elliott) > 2. CD20 for Mouse Tissue (Elizabeth Cameron) > 3. gloves (Perry, Margaret) > 4. Re: CD20 for Mouse Tissue (Eva Permaul) > 5. HT study material (Gomez, Milton) > 6. CJD processing (Jennifer MacDonald) > 7. Procollagen (Susan Foreman) > 8. nails staying on slides (kcastillo@frii.com) > ---------------------------------------------------------------------- Message: 1 > Date: Wed, 22 Aug 2012 11:20:02 -0700 > From: "Mark Elliott" > Subject: [Histonet] Versican antibody for dog tissue > To: > Message-ID: <5034C062020000D6000506CC@mail.mrl.ubc.ca> > Content-Type: text/plain; charset=US-ASCII > Can anyone recommend a versican antibody that works on dog tissue? Thanks > Mark > ***CONFIDENTIALITY NOTICE*** > This electronic message and any attachments are intended only for the use > of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. > ------------------------------ > Message: 2 > Date: Wed, 22 Aug 2012 18:48:43 +0000 > From: Elizabeth Cameron > Subject: [Histonet] CD20 for Mouse Tissue > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > Does anyone know of a CD20 antibody that works well on paraffin embedded mouse tissue? > Thanks! > -Liz > The information in this email, including attachments, may be confidential > and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as > possible. > ------------------------------ > Message: 3 > Date: Wed, 22 Aug 2012 18:48:00 +0000 > From: "Perry, Margaret" > Subject: [Histonet] gloves > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25F4FBA34BE9D142964ECC4525B82AEE041860@SDSU-EX03.jacks.local> > Content-Type: text/plain; charset="us-ascii" > Where do you buy the Sempermed gloves? > Margaret Perry HT(ASCP) > Veterinary & Biomedical Sciences Department > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > ------------------------------ > Message: 4 > Date: Wed, 22 Aug 2012 15:25:13 -0400 > From: Eva Permaul > Subject: Re: [Histonet] CD20 for Mouse Tissue > To: Elizabeth Cameron , histonet > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > Hi Liz, > We just used CD20 cat.no. 14-0201 from eBioscience on mouse livers and spleen. It worked great. Email me separate if you would like to send you the protocol. > Eva > Eva Permaul > Georgetown University > On Wed, Aug 22, 2012 at 2:48 PM, Elizabeth Cameron < > Elizabeth.Cameron@jax.org> wrote: >> Does anyone know of a CD20 antibody that works well on paraffin embedded >> mouse tissue? >> Thanks! >> -Liz >> The information in this email, including attachments, may be >> confidential >> and is intended solely for the addressee(s). If you believe you received >> this email by mistake, please notify the sender by return email as soon as >> possible. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ > Message: 5 > Date: Wed, 22 Aug 2012 23:15:47 +0000 > From: "Gomez, Milton" > Subject: [Histonet] HT study material > To: " histonet@lists.utsouthwestern.edu" > > Message-ID: > <7A7F4F00BF2D2D46A158AE61E0D2B6302A1F1C48@MSGWCDCPMB27.nyumc.org> > Content-Type: text/plain; charset="iso-8859-1" > Dear Histonetters, > Does anyone have study material that would like to either sell and or share for the HT (ASCP) examination? > Thank you very much in advance, > MG > ------------------------------ > Message: 6 > Date: Wed, 22 Aug 2012 22:31:21 -0700 > From: Jennifer MacDonald > Subject: [Histonet] CJD processing > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > Does anyone know of a lab in Southern California that is equipped to handle CJD (suspected) cases? > Thank you, > Jennifer > ------------------------------ > Message: 7 > Date: Thu, 23 Aug 2012 07:51:05 -0400 > From: "Susan Foreman" > Subject: [Histonet] Procollagen > To: > Message-ID: <000301cd8125$93825e20$ba871a60$@com> > Content-Type: text/plain; charset="us-ascii" > One of our pathologists is looking to send a case out for Procollagen IHC > staining. Do you know who is offering this testing? > Susan Foreman, HT (ASCP) > KDL Pathology > 315 Erin Drive > Knoxville, TN 37919 > (865)584-1933 > ------------------------------ > Message: 8 > Date: Thu, 23 Aug 2012 08:29:17 -0600 > From: kcastillo@frii.com > Subject: [Histonet] nails staying on slides > To: > Message-ID: <9ccaa6bf0d6385dd63fa07dce03d8778@frii.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > Hi Everyone in Histoland, > Having problems keeping my nails on plus slides when staining. They of course fall off during the PAS staining process. Can anyone help me please, thank you! Kristy > ------------------------------ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > End of Histonet Digest, Vol 105, Issue 29 > ***************************************** From azdudley <@t> hotmail.com Thu Aug 23 14:34:07 2012 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Aug 23 14:34:16 2012 Subject: [Histonet] CJD processing In-Reply-To: References: Message-ID: Jennifer, We have in our procedure to send any sucpected specimen to the National Prion Disease Pathology Surveillance center at Case Western University in Cleveland Ohio. the phone number is 216-368-0587. Hope this may help. Anita Dudley > To: histonet@lists.utsouthwestern.edu > From: JMacDonald@mtsac.edu > Date: Wed, 22 Aug 2012 22:31:21 -0700 > Subject: [Histonet] CJD processing > > Does anyone know of a lab in Southern California that is equipped to > handle CJD (suspected) cases? > Thank you, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Thu Aug 23 15:02:19 2012 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu Aug 23 15:02:42 2012 Subject: [Histonet] nails staying on slides In-Reply-To: <9ccaa6bf0d6385dd63fa07dce03d8778@frii.com> References: <9ccaa6bf0d6385dd63fa07dce03d8778@frii.com> Message-ID: <5036540B0200003400016317@mcohio.org> I use a 5% solution of Titebond 2 wood glue to coat slides. You should be able to find it at your local hardware store. Works well for me on bone and bloody tissue, Occasionally I need to microwave dry slides of very dense bone (skull, inner ear). >>> 8/23/2012 10:29 AM >>> Hi Everyone in Histoland, Having problems keeping my nails on plus slides when staining. They of course fall off during the PAS staining process. Can anyone help me please, thank you! Kristy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Fri Aug 24 07:30:20 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Fri Aug 24 07:30:23 2012 Subject: [Histonet] Osmium tetroxide staining for lipids Message-ID: Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila From jengirl1014 <@t> yahoo.com Fri Aug 24 07:50:52 2012 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Aug 24 07:50:59 2012 Subject: [Histonet] folds in tissue Message-ID: <1345812652.97392.YahooMailNeo@web160303.mail.bf1.yahoo.com> Good morning/afternoon/evening Histonetters! ? I'm having a problem with my tissue folding.? It's tiny folds in mouse tissue that are too little to see without the use of a microscope.? I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope. ? I'm using distilled water in my water bath and it's set to about 40 degrees Celcius. ? All help is appreciated!? Thank you all so much for your time! ? Regards, ? Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? 410-614-0131 fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu From micropathlabs <@t> yahoo.com Fri Aug 24 08:00:46 2012 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Aug 24 08:00:53 2012 Subject: [Histonet] folds in tissue In-Reply-To: <1345812652.97392.YahooMailNeo@web160303.mail.bf1.yahoo.com> References: <1345812652.97392.YahooMailNeo@web160303.mail.bf1.yahoo.com> Message-ID: <1345813246.20575.YahooMailNeo@web122002.mail.ne1.yahoo.com> Try floating your sections on room temperature (in a shallow dish)?water then carefully, on a slide, transfer to the waterbath. Sections will spread better when they hit the warm water. Good luck! Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From: Jennifer Sipes To: Histonet Sent: Friday, August 24, 2012 8:50 AM Subject: [Histonet] folds in tissue Good morning/afternoon/evening Histonetters! ? I'm having a problem with my tissue folding.? It's tiny folds in mouse tissue that are too little to see without the use of a microscope.? I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope. ? I'm using distilled water in my water bath and it's set to about 40 degrees Celcius. ? All help is appreciated!? Thank you all so much for your time! ? Regards, ? Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? 410-614-0131 fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Fri Aug 24 08:08:12 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Aug 24 08:09:38 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388DAE@UTHCMS1.uthouston.edu> Sheila Hi I am assuming that you are using frozen sections? The easiest way is to put a small amount of aqueous osmium tetroxide in the bottom of a dish that has a tight fitting lid. Place the slide with section into the dish but not touching the fluid. Place tightly fitting lid and leave - not sure how long, probably 20- 30 minutes should do it although can leave longer. Then simply rinse and that's it. The osmium vapor fixes the lipid so that it is not important to have any buffer in the solution, can just use straightforward osmium tetroxide in distilled water. The osmium black that forms is insoluble in most solvents. You should do this in a fume hood as the vapor will fix your nasal mucosa if you are not careful. Hope that your pathologist realizes that although the osmium will stain the lipid it makes staining of other components very difficult. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey [sadey@hotmail.ca] Sent: Friday, August 24, 2012 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Fri Aug 24 09:08:26 2012 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Aug 24 09:08:35 2012 Subject: [Histonet] folds in tissue In-Reply-To: <1345813246.20575.YahooMailNeo@web122002.mail.ne1.yahoo.com> References: <1345812652.97392.YahooMailNeo@web160303.mail.bf1.yahoo.com>, <1345813246.20575.YahooMailNeo@web122002.mail.ne1.yahoo.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF55E81F@EXCHANGEMB1.hmc.hurleymc.com> I know that animal tissue is very different than human and we deal only with human. But we share the same issues here. We have found that floating the tissue in a room temperature dish of 30% alcohol and then transferring them to the water bath has helped us. Interesting to hear what others are doing!! I'll have to try the room temp water! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Friday, August 24, 2012 9:00 AM To: Jennifer Sipes; Histonet Subject: Re: [Histonet] folds in tissue Try floating your sections on room temperature (in a shallow dish) water then carefully, on a slide, transfer to the waterbath. Sections will spread better when they hit the warm water. Good luck! Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From: Jennifer Sipes To: Histonet Sent: Friday, August 24, 2012 8:50 AM Subject: [Histonet] folds in tissue Good morning/afternoon/evening Histonetters! I'm having a problem with my tissue folding. It's tiny folds in mouse tissue that are too little to see without the use of a microscope. I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope. I'm using distilled water in my water bath and it's set to about 40 degrees Celcius. All help is appreciated! Thank you all so much for your time! Regards, Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 fax: 410-955-9677 cell: 443-631-6361 e-mail: jsipes1@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Fri Aug 24 09:30:50 2012 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Aug 24 09:30:59 2012 Subject: [Histonet] Job opportunity Message-ID: <503749CA020000AF0000A125@nodcdmg2.no.trinity-health.org> 238 bed acute care hospital with affiliated reference laboratory in the MidWest is currently looking for a histotechnician or histotechnologist (ASCP registered or equivalent preferred). Responsibilities will include, but are not limited to, specimen processing of surgical and cytology specimens, cutting, embedding, and both basic H & E stains as well as special stains, IHC, ISH stains. Works closely with the pathologist while grossing and performing frozen sections. Please apply at our web-site http://www.healthcaresource.com/mercysiouxcity_i/index.cfm?fuseaction=search.categoryList&template=dsp_job_categories.cfm or call Pat Cable (712-233-5243) for more information. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com From rjbuesa <@t> yahoo.com Fri Aug 24 09:31:38 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 24 09:34:32 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: References: Message-ID: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing. The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. This is the most current measure. On the other hand, as you point out, osmium tetroxide is used in electron microscopy. Ren? J. ________________________________ From: Sheila Adey To: "histonet@lists.utsouthwestern.edu" Sent: Friday, August 24, 2012 8:30 AM Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 24 09:36:20 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 24 09:36:28 2012 Subject: [Histonet] folds in tissue In-Reply-To: <1345812652.97392.YahooMailNeo@web160303.mail.bf1.yahoo.com> References: <1345812652.97392.YahooMailNeo@web160303.mail.bf1.yahoo.com> Message-ID: <1345818980.95438.YahooMailNeo@web121403.mail.ne1.yahoo.com> For this difficult tissue, and for any other for that matter, add to your regular round water bath at 45?C, 2 mL of liquid dish washer soap. The soap will reduce the surface tension of the water and will allow the sections to extend completely, eliminating any folds. Ren? J. ________________________________ From: Jennifer Sipes To: Histonet Sent: Friday, August 24, 2012 8:50 AM Subject: [Histonet] folds in tissue Good morning/afternoon/evening Histonetters! ? I'm having a problem with my tissue folding.? It's tiny folds in mouse tissue that are too little to see without the use of a microscope.? I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope. ? I'm using distilled water in my water bath and it's set to about 40 degrees Celcius. ? All help is appreciated!? Thank you all so much for your time! ? Regards, ? Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? 410-614-0131 fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Aug 24 09:42:34 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Aug 24 09:42:50 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> References: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939571416C@chimsx08.CHI.catholichealth.net> I agree w Rene on this one - do not use this stuff if you do not have to - and in 2012 (or at least in the last 20 years or more) - you do not HAVE to! - Oil Red O is far safer - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 24, 2012 9:32 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Osmium tetroxide staining for lipids Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing. The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. This is the most current measure. On the other hand, as you point out, osmium tetroxide is used in electron microscopy. Ren? J. ________________________________ From: Sheila Adey To: "histonet@lists.utsouthwestern.edu" Sent: Friday, August 24, 2012 8:30 AM Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Ronald.Houston <@t> nationwidechildrens.org Fri Aug 24 09:45:01 2012 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Aug 24 09:45:15 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388DAE@UTHCMS1.uthouston.edu> References: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388DAE@UTHCMS1.uthouston.edu> Message-ID: Barry, I suspect they are wanting to employ this on paraffin sections as Sheila mentions using it on routine 4 micron sections> If that is the case, there are published articles using Sudan Black B and Oil Red O (Histopathology 2002, 41, 75-79) in paraffin sections, and I believe Tim Morken has used OsO4 in paraffin sections. I have heard of fixed blocks being impregnated with Osmium before processing and then being sectioned; the Osmium bound lipids are stained. I've even used en-bloc Osmium staining for degenerating myelin with the Marchi technique (now there's a walk down memory lane!!) All that being said, I would not recommend using OsO4 in a lab that has not previously used it, as it is an extremely hazardous substance to use, and there are other alternatives. As a matter of interest Sheila, did your pathologist mention what he was wanting to specifically use the osmium for, or is this just some stab in the dark because he read it in some obscure journal? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, August 24, 2012 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Osmium tetroxide staining for lipids Sheila Hi I am assuming that you are using frozen sections? The easiest way is to put a small amount of aqueous osmium tetroxide in the bottom of a dish that has a tight fitting lid. Place the slide with section into the dish but not touching the fluid. Place tightly fitting lid and leave - not sure how long, probably 20- 30 minutes should do it although can leave longer. Then simply rinse and that's it. The osmium vapor fixes the lipid so that it is not important to have any buffer in the solution, can just use straightforward osmium tetroxide in distilled water. The osmium black that forms is insoluble in most solvents. You should do this in a fume hood as the vapor will fix your nasal mucosa if you are not careful. Hope that your pathologist realizes that although the osmium will stain the lipid it makes staining of other components very difficult. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey [sadey@hotmail.ca] Sent: Friday, August 24, 2012 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From sadey <@t> hotmail.ca Fri Aug 24 09:50:40 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Fri Aug 24 09:50:47 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939571416C@chimsx08.CHI.catholichealth.net> References: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com>, <4940DF6D1C5FDF48931B6966AAEF939571416C@chimsx08.CHI.catholichealth.net> Message-ID: Thank you so much for this response. :) > Subject: RE: [Histonet] Osmium tetroxide staining for lipids > Date: Fri, 24 Aug 2012 08:42:34 -0600 > From: billodonnell@catholichealth.net > To: rjbuesa@yahoo.com; sadey@hotmail.ca; histonet@lists.utsouthwestern.edu > > I agree w Rene on this one - do not use this stuff if you do not have to - and in 2012 (or at least in the last 20 years or more) - you do not HAVE to! - Oil Red O is far safer - Bill > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, August 24, 2012 9:32 AM > To: Sheila Adey; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Osmium tetroxide staining for lipids > > Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing. > The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. > Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. > This is the most current measure. > On the other hand, as you point out, osmium tetroxide is used in electron microscopy. > Ren? J. > > > ________________________________ > From: Sheila Adey > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, August 24, 2012 8:30 AM > Subject: [Histonet] Osmium tetroxide staining for lipids > > > Hi Everyone: > > One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. > Is anyone using this procedure for routine 4 micrometer sections? > > Thanks > :) > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From mcauliff <@t> umdnj.edu Fri Aug 24 10:04:04 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 24 10:02:19 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: References: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> <4940DF6D1C5FDF48931B6966AAEF939571416C@chimsx08.CHI.catholichealth.net> Message-ID: <503797E4.7030104@umdnj.edu> Two other arguments against osmium, it is very expensive and is must be disposed of as hazardous waste. Geoff On 8/24/2012 10:50 AM, Sheila Adey wrote: > Thank you so much for this response. :) > > >> Subject: RE: [Histonet] Osmium tetroxide staining for lipids >> Date: Fri, 24 Aug 2012 08:42:34 -0600 >> From: billodonnell@catholichealth.net >> To: rjbuesa@yahoo.com; sadey@hotmail.ca; histonet@lists.utsouthwestern.edu >> >> I agree w Rene on this one - do not use this stuff if you do not have to - and in 2012 (or at least in the last 20 years or more) - you do not HAVE to! - Oil Red O is far safer - Bill >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >> Sent: Friday, August 24, 2012 9:32 AM >> To: Sheila Adey; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Osmium tetroxide staining for lipids >> >> Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing. >> The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. >> Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. >> This is the most current measure. >> On the other hand, as you point out, osmium tetroxide is used in electron microscopy. >> Ren? J. >> >> >> ________________________________ >> From: Sheila Adey >> To: "histonet@lists.utsouthwestern.edu" >> Sent: Friday, August 24, 2012 8:30 AM >> Subject: [Histonet] Osmium tetroxide staining for lipids >> >> >> Hi Everyone: >> >> One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. >> Is anyone using this procedure for routine 4 micrometer sections? >> >> Thanks >> :) >> Sheila _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From asmith <@t> mail.barry.edu Fri Aug 24 10:14:59 2012 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Aug 24 10:15:12 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> References: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> Message-ID: Osmium tetroxide solutions will fix your skin on contact, but skin grows back. Osmium tetroxide fumes will fix your corneas which are irreplaceable. Osmium tetroxide is dangerous, but it is manageable. First, make sure your fume hood draws really, really well. Put 100 ml of pH 7.2 phosphate-buffered saline into a 150 or 200 ml glass bottle. Score a 1 gram ampoule of osmium tetroxide with a file. Drop the ampoule into the bottle, close the bottle tightly, and shake it until the ampoule breaks. Let the osmium tetroxide dissolve overnight. Use as little as possible of this solution on frozen sections IN THE FUME HOOD. The glass door should be as far down as is practical. Keep your head out of the fume hood! Only your hands should be inside. Used osmium tetroxide solution and the first rinse should be saved in a tightly closed glass bottle and given to a waste hauler. Since it has salvage value, some waste haulers will take osmium tetroxide without charge. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 24, 2012 10:32 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Osmium tetroxide staining for lipids Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing. The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. This is the most current measure. On the other hand, as you point out, osmium tetroxide is used in electron microscopy. Ren? J. ________________________________ From: Sheila Adey To: "histonet@lists.utsouthwestern.edu" Sent: Friday, August 24, 2012 8:30 AM Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cebass <@t> buffalo.edu Fri Aug 24 10:34:12 2012 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Fri Aug 24 10:34:21 2012 Subject: [Histonet] source for plus slides and labels Message-ID: Hi Everyone, I'm trying to buy super frost plus slides (or color frost) for adhering brain sections. Fisher is very confusing on this, and I don't really remember buying slides for $1 each. Can someone recommend a source for plus slides that is cheaper? Also, I just started using labels for my rather low throughput immunos, I have a brady tls pclink printer, and the labels print well, but they didn't hold up to the xylenes. Can someone recommend a label for this printer? Any real world experience? Our protocol calls for overnight in xylenes. Finally, any recommendation on mounting media? I've always used per mount, but a lot of folks seem to be using xylenes free media. I'm doing mostly DAB immunos, so any advice is appreciate! thanks, Caroline Bass From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 24 10:45:27 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 24 10:47:34 2012 Subject: [Histonet] RE: source for plus slides and labels In-Reply-To: References: Message-ID: We use the Leica Biosystems X-tra slides with a lot of success for Brain and bone. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bass, Caroline [cebass@buffalo.edu] Sent: Friday, August 24, 2012 11:34 AM To: Histonet Subject: [Histonet] source for plus slides and labels Hi Everyone, I'm trying to buy super frost plus slides (or color frost) for adhering brain sections. Fisher is very confusing on this, and I don't really remember buying slides for $1 each. Can someone recommend a source for plus slides that is cheaper? Also, I just started using labels for my rather low throughput immunos, I have a brady tls pclink printer, and the labels print well, but they didn't hold up to the xylenes. Can someone recommend a label for this printer? Any real world experience? Our protocol calls for overnight in xylenes. Finally, any recommendation on mounting media? I've always used per mount, but a lot of folks seem to be using xylenes free media. I'm doing mostly DAB immunos, so any advice is appreciate! thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Fri Aug 24 10:49:20 2012 From: ewj <@t> pigsqq.org (E. Wayne Johnson) Date: Fri Aug 24 10:49:37 2012 Subject: [Histonet] source for plus slides and labels In-Reply-To: References: Message-ID: <5037A280.4060802@pigsqq.org> We can buy locally made plus slides for $1.25 per box of 50 and we can buy imported Leica slides for about $1 per slide. The Leica slides are very nice indeed, but the locally made ones provide similar performance. E. Wayne Johnson Enruikang AgTech Beijing. On 8/24/2012 11:34 PM, Bass, Caroline wrote: > Hi Everyone, > > I'm trying to buy super frost plus slides (or color frost) for adhering brain sections. Fisher is very confusing on this, and I don't really remember buying slides for $1 each. Can someone recommend a source for plus slides that is cheaper? > > Also, I just started using labels for my rather low throughput immunos, I have a brady tls pclink printer, and the labels print well, but they didn't hold up to the xylenes. Can someone recommend a label for this printer? Any real world experience? Our protocol calls for overnight in xylenes. > > Finally, any recommendation on mounting media? I've always used per mount, but a lot of folks seem to be using xylenes free media. I'm doing mostly DAB immunos, so any advice is appreciate! > > thanks, > > Caroline Bass > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 24 11:01:28 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 24 11:02:14 2012 Subject: [Histonet] source for plus slides and labels In-Reply-To: <5037A280.4060802@pigsqq.org> References: , <5037A280.4060802@pigsqq.org> Message-ID: I don't pay $1 per slide for the leica slides....so you might want to ask your salesrep. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson [ewj@pigsqq.org] Sent: Friday, August 24, 2012 11:49 AM To: Bass, Caroline Cc: Histonet Subject: Re: [Histonet] source for plus slides and labels We can buy locally made plus slides for $1.25 per box of 50 and we can buy imported Leica slides for about $1 per slide. The Leica slides are very nice indeed, but the locally made ones provide similar performance. E. Wayne Johnson Enruikang AgTech Beijing. On 8/24/2012 11:34 PM, Bass, Caroline wrote: > Hi Everyone, > > I'm trying to buy super frost plus slides (or color frost) for adhering brain sections. Fisher is very confusing on this, and I don't really remember buying slides for $1 each. Can someone recommend a source for plus slides that is cheaper? > > Also, I just started using labels for my rather low throughput immunos, I have a brady tls pclink printer, and the labels print well, but they didn't hold up to the xylenes. Can someone recommend a label for this printer? Any real world experience? Our protocol calls for overnight in xylenes. > > Finally, any recommendation on mounting media? I've always used per mount, but a lot of folks seem to be using xylenes free media. I'm doing mostly DAB immunos, so any advice is appreciate! > > thanks, > > Caroline Bass > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 24 11:13:29 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Aug 24 11:12:50 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FFFF4A@ex07.net.ucsf.edu> Sheila, You can't normally use osmium lipid fixation for paraffin sections because the lipid you want to see will usually have been dissolved out by the paraffin processing. You need to either post-fix in osmium (after formalin) before paraffin processing or use frozen sections. As mentioned, once fixed in osmium other histology stains don't work so well because the tissue is rendered black and the osmium interferes with many stains. Barry Rittman gave the procedure for frozen sections. You MUST use it in an exterior-vented chemical fume hood (NOT one with filtered air blowing out the top as in a biosafety cabinet or bench top unit). Using osmium is not worth the trouble for one-off or even occasional cases. It is time consuming to prepare, must be stored in the dark in well-sealed triple containers each sealed with parafilm wrap or the vapor will escape and blacken everything in the refridgerator or cupboard. It is not something to take lightly. If you can smell it, it is fixing your nose! Disposal is a hassle as well. If you have access to an EM lab then they can do it for you and save you the hassle. Otherwise use the other methods mentioned. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, August 24, 2012 5:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From swong <@t> cleavebio.com Fri Aug 24 13:11:06 2012 From: swong <@t> cleavebio.com (Steve Wong) Date: Fri Aug 24 13:11:16 2012 Subject: [Histonet] BMDS transponder chips Message-ID: Hello, Does anyone have experience with reusing the BMDS transponder chips? Most importantly, are they autoclaveable? If not, I'm looking for suggestions to sterilize them (and the inplantation device) for reimplantation. Thanks, Steve From Barry.R.Rittman <@t> uth.tmc.edu Fri Aug 24 18:50:36 2012 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Aug 24 18:50:41 2012 Subject: [Histonet] Osmium tetroxide staining for lipids In-Reply-To: References: <1345818698.81868.YahooMailNeo@web121404.mail.ne1.yahoo.com> <4940DF6D1C5FDF48931B6966AAEF939571416C@chimsx08.CHI.catholichealth.net>, Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C1388DB3@UTHCMS1.uthouston.edu> Shelia I am sorry that you have received conflicting information on this question. What we should all have asked you to start with is what did you finally want to end up with? We all assumed that you just wanted to stain frozen sections. If just looking at frozen section then I do agree that Oil red O is as good as osmium tetroxide and much easier and safer to use. If however you want to carry out the process on a block of tissue and end up with paraffin sections osmium tetroxide is I believe the method of choice. Sudan Black does not necessarily stain all lipids in paraffin sections. Osmium tetroxide is very dangerous, but if used with all recommended precautions in a fume hood will be just fine - if not then there are a lot of electron microscopists that will be out of business. We all use a lot of dangerous chemicals and also chemicals that appear to have minimal effects but many of these not been tested for their accumulative effects over years of exposure. It behoves us to treat most chemicals with respect and with any extra precaution such as necessary with osmium tetroxide. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey [sadey@hotmail.ca] Sent: Friday, August 24, 2012 9:50 AM To: billodonnell@catholichealth.net; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Osmium tetroxide staining for lipids Thank you so much for this response. :) > Subject: RE: [Histonet] Osmium tetroxide staining for lipids > Date: Fri, 24 Aug 2012 08:42:34 -0600 > From: billodonnell@catholichealth.net > To: rjbuesa@yahoo.com; sadey@hotmail.ca; histonet@lists.utsouthwestern.edu > > I agree w Rene on this one - do not use this stuff if you do not have to - and in 2012 (or at least in the last 20 years or more) - you do not HAVE to! - Oil Red O is far safer - Bill > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, August 24, 2012 9:32 AM > To: Sheila Adey; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Osmium tetroxide staining for lipids > > Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing. > The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. > Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. > This is the most current measure. > On the other hand, as you point out, osmium tetroxide is used in electron microscopy. > Ren? J. > > > ________________________________ > From: Sheila Adey > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, August 24, 2012 8:30 AM > Subject: [Histonet] Osmium tetroxide staining for lipids > > > Hi Everyone: > > One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. > Is anyone using this procedure for routine 4 micrometer sections? > > Thanks > :) > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Mon Aug 27 12:12:14 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Mon Aug 27 12:12:20 2012 Subject: [Histonet] Ergonomics Message-ID: Hi Everyone, Has anyone ever used the adjustable microtome handle from Newcomer Supply it is called, "Histo comfort grip handle"? If you have, what did you think? How many of you are using a foot pedal if your microtome comes with it. I am getting repetitive motion stress in mainly my shoulder and a little in the elbow and wrist. I guess that is what happens as we get older and have been doing this for a long time. I have used my foot pedal a couple of time but then I lose the feel when I am facing into the block and sometimes finessing a difficult ribbon. Your thoughts on this would be greatly appreciated. Thanks Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From brian <@t> prometheushealthcare.com Mon Aug 27 14:24:34 2012 From: brian <@t> prometheushealthcare.com (Brian-Prometheus) Date: Mon Aug 27 14:24:50 2012 Subject: [Histonet] Histotech opening in South Carolina Message-ID: <02fd01cd8489$9c9b93c0$d5d2bb40$@com> My name is Brian Feldman and I work with Prometheus Healthcare. We specialize in the placement of laboratory professionals nationwide. We are currently working on a histotech position with a full-service anatomic pathology lab near Hilton Head, SC. This position has an early morning start time at 1am and will be doing general histology as well as IHC. Please contact me today for immediate consideration. Thanks! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From we3smitty <@t> yahoo.com Mon Aug 27 15:52:41 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Mon Aug 27 15:52:44 2012 Subject: [Histonet] Remote processor alarms Message-ID: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith From HornHV <@t> archildrens.org Mon Aug 27 16:37:05 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 27 16:37:10 2012 Subject: [Histonet] Remote processor alarms In-Reply-To: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> References: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A17F3@EVS1.archildrens.org> Our hospital monitors ours. Our processor is connected to building automation and if it alarms off hours they call me. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 3:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From TMcNemar <@t> lmhealth.org Tue Aug 28 04:37:14 2012 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Aug 28 04:37:20 2012 Subject: [Histonet] Remote processor alarms In-Reply-To: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> References: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From ibernard <@t> uab.edu Tue Aug 28 05:44:11 2012 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Tue Aug 28 05:44:24 2012 Subject: [Histonet] Let's Talk CAP Inspections In-Reply-To: References: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: I trust all of us will hopefully benefit from this line of discussion: In 2013 our lab will undergo our 2013 CAP inspection. The Cyto (Non-Gyn)-pathology, and Anatomic(Surgical only) Pathology and now the new "Commons" checklist will be used. Over the next weeks, in an effort to prepare, and to get feedback to CAP questions that tend to be subject-to-interpretation or subjective (since inspectors are our peers) versus objective questions (that are straight forward (or "elementary my dear friend"), I will present questions with hopes of obtaining interpretations from you, my fellow subject matter experts and fellow inspectors. By the way, this will be my first CAP inspection as sole manager of our lab. I also think volunteering to serve as an inspector will help the process as well. Look out, I may be coming your way. IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, August 28, 2012 4:37 AM To: 'angela smith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remote processor alarms We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmendell <@t> goldbergmd.net Tue Aug 28 07:28:20 2012 From: kmendell <@t> goldbergmd.net (MaryK Mendell) Date: Tue Aug 28 07:29:46 2012 Subject: [Histonet] RE: Let's Talk CAP Inspections In-Reply-To: References: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> , Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D357@EXMBX01.mmeprod.cbeyond> I agree on being an inspector, I learned so much doing that. You can also share ideas when you see people who could make a few changes to save time. Good Luck and welcome your questions. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard [ibernard@uab.edu] Sent: Tuesday, August 28, 2012 6:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Let's Talk CAP Inspections I trust all of us will hopefully benefit from this line of discussion: In 2013 our lab will undergo our 2013 CAP inspection. The Cyto (Non-Gyn)-pathology, and Anatomic(Surgical only) Pathology and now the new "Commons" checklist will be used. Over the next weeks, in an effort to prepare, and to get feedback to CAP questions that tend to be subject-to-interpretation or subjective (since inspectors are our peers) versus objective questions (that are straight forward (or "elementary my dear friend"), I will present questions with hopes of obtaining interpretations from you, my fellow subject matter experts and fellow inspectors. By the way, this will be my first CAP inspection as sole manager of our lab. I also think volunteering to serve as an inspector will help the process as well. Look out, I may be coming your way. IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, August 28, 2012 4:37 AM To: 'angela smith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remote processor alarms We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Tue Aug 28 08:14:00 2012 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Aug 28 08:14:07 2012 Subject: [Histonet] Great Colorado HT Position !!!! Message-ID: <6F33D8418806044682A391273399860F0B3DD711@s-irv-ex301.PathologyPartners.intranet> Great part time opportunities' for Histotechnician's in Centennial, Colorado! Colorado GI Pathology is looking for certified HT's or HTL's to join their existing laboratory staff. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a part time that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com From mhale <@t> MiracaLS.com Tue Aug 28 08:18:32 2012 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Aug 28 08:18:41 2012 Subject: [Histonet] Great Louisiana Position Message-ID: <6F33D8418806044682A391273399860F0B3DD712@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Louisiana Dermatology in Baton Rouge, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing dermatology specimens * HT ASCP Certified , minimum 3 years working in a histology laboratory , 1-2 years supervisor experience preferred Duties include: * Creation and maintenance of policies and procedures * Manage laboratory budget and supplies * Maintenance of QC documents' * Routine histology duties This is a full-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com From ChaseM <@t> childrensdayton.org Tue Aug 28 08:44:22 2012 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Tue Aug 28 08:44:33 2012 Subject: [Histonet] RE: Let's Talk CAP Inspections In-Reply-To: References: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: <187FB57B597CB24F9F59F357264999F715B01411@PEXCHNGDAG01.cmc-dayton.org> We just received the "All Common Checklist" all the questions from COM 40300 Analytic Accuracy/Precision to the end seem to be something for the clinical lab. How are some of you dealing with the following questions? COM.40300 Analytic Accuracy/Precision Phase II The laboratory verifies or establishes analytic accuracy and precision for each test. NOTE: Where current technology permits, accuracy is established by comparing results to a definitive or reference method, or may be verified by comparing results to an established comparative method. Use of reference materials or other materials with known concentrations or activities is suggested in establishing or verifying accuracy. Precision is established by repeat measurement of samples at varying concentrations or activities within-run and between-run over a period of time. Evidence of Compliance: ? Written procedure for determining method performance characteristics, including accuracy/precision AND ? Records of verification or establishment of analytic accuracy and precision for each test COM.40400 Analytic Sensitivity Phase II The laboratory verifies or establishes the analytic sensitivity (lower detection limit) of each assay, as applicable. NOTE: For FDA-cleared/approved tests, documentation may consist of data from manufacturers or the published literature. Evidence of Compliance: ? Written procedure for determining method performance characteristics, including analytic sensitivity AND ? Records of verification or establishment of analytic sensitivity for each assay COM.40500 Analytic Interferences Phase II The laboratory verifies or establishes analytic interferences for each test. NOTE: Interfering substances may pose a significant problem to the clinical laboratory and healthcare providers who may be misled by laboratory results that do not reflect patient clinical status. The laboratory must be aware of common interferences by performing studies or having available studies performed elsewhere (such as by the instrument-reagent manufacturer). Evidence of Compliance: ? Written procedure for determining method performance characteristics, including analytic interferences AND ? Records of verification or establishment of analytic interferences for each test COM.40600 Reportable Range Phase II The reportable range (analytic measurement range) is verified/established for each analytic procedure before implementation. NOTE: The analytic measurement range (AMR) is the range of analyte values that a method can directly measure on the specimen without any dilution or concentration. Expanded definitions and details of the AMR are provided in some of the section-specific checklists (e.g. Chemistry). Verification of the AMR may not apply to certain assays (for example, in immunology and coagulation). The limits of the AMR are based on meeting accuracy and precision requirements such as the minimal limit of quantification or sensitivity, when applicable. In some cases, clinically relevant limits may be narrower than the potential analytical range, and the clinically relevant limit would be used as the limit of the reportable range. Evidence of Compliance: ? Written policy for determining method performance characteristics, including reportable range AND ? Records of verification or establishment of reportable ranges for each test COM.40700 Method Performance Specifications Availability Phase II The laboratory's current test methods, including performance specifications and supporting validation data (analytic accuracy, precision, analytic sensitivity, interferences, reference range, and reportable range, as acceptable), are available to clients of the laboratory and to the inspection team upon request. NOTE: The laboratory must also provide data on clinical validity, if available, to clients upon request. The CAP inspection team is instructed to use the validation data solely for accreditation purposes. The laboratory may at its option require clients to agree to treat validation data as confidential and not to share such data with any other party except as required by law. COM.40800 Analytic Methodology Changes Phase II If the laboratory changes its analytic methodology so that test results or their interpretations may be SIGNIFICANTLY different, the change is explained to clients. NOTE: This requirement can be accomplished in any of several different ways, depending on local circumstances. Some methods include directed mailings, laboratory newsletters or part of the test report itself. Evidence of Compliance: ? Records such as directed mailings, laboratory newsletters or comment on the patient report advising of the change COM.50000 Reference Intervals Phase II The laboratory establishes or verifies its reference intervals (normal values). NOTE: Reference intervals are important to allow a clinician to assess patient results against an appropriate population. The reference range must be established or verified for each analyte and specimen source (e.g. blood, urine, cerebrospinal fluid), when appropriate. For many analytes (e.g. therapeutic drugs and CSF total protein), literature references or a manufacturer's package insert information may be appropriate. Evidence of Compliance: ? Record of reference range study OR records of verification of manufacturer's stated range when reference range study is not practical (e.g. unavailable normal population) OR other methods approved by the laboratory director COM.50100 Reference Interval Evaluation Phase II The laboratory evaluates the appropriateness of its reference intervals and takes corrective action if necessary. NOTE: Criteria for evaluation of reference intervals include: 1. Introduction of a new analyte to the test repertoire 2. Change of analytic methodology 3. Change in patient population If it is determined that the range is no longer appropriate for the patient population, corrective action must be taken. Evidence of Compliance: ? Records of evaluation and corrective action, if indicated -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Tuesday, August 28, 2012 6:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Let's Talk CAP Inspections I trust all of us will hopefully benefit from this line of discussion: In 2013 our lab will undergo our 2013 CAP inspection. The Cyto (Non-Gyn)-pathology, and Anatomic(Surgical only) Pathology and now the new "Commons" checklist will be used. Over the next weeks, in an effort to prepare, and to get feedback to CAP questions that tend to be subject-to-interpretation or subjective (since inspectors are our peers) versus objective questions (that are straight forward (or "elementary my dear friend"), I will present questions with hopes of obtaining interpretations from you, my fellow subject matter experts and fellow inspectors. By the way, this will be my first CAP inspection as sole manager of our lab. I also think volunteering to serve as an inspector will help the process as well. Look out, I may be coming your way. IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, August 28, 2012 4:37 AM To: 'angela smith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remote processor alarms We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. From Joyce.Weems <@t> emoryhealthcare.org Tue Aug 28 09:44:13 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Aug 28 09:44:23 2012 Subject: [Histonet] Remote processor alarms In-Reply-To: References: <1346100761.66363.YahooMailClassic@web125402.mail.ne1.yahoo.com> Message-ID: Same here... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, August 28, 2012 5:37 AM To: 'angela smith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remote processor alarms We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsfmedctr.org Tue Aug 28 10:46:32 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Aug 28 10:45:43 2012 Subject: [Histonet] downloads available on these questions RE: Let's Talk CAP Inspections Message-ID: <761E2B5697F795489C8710BCC72141FF010322@ex07.net.ucsf.edu> You can download a powerpoint I made for validation presentations at NSH and ASCP meetings. Part of that presentation covers the issues mentioned below on how to relate regulations written primarily for analytical clinical labs to the histo and IHC labs. This is a Yahoo group. Join for free and download the presentation "ASCP 2011 IHC QC Oct 20 2011 v10212011.pdf" (and you won't get any emails from me about the group. It is just a convenient download site). http://pets.groups.yahoo.com/group/histoinfo/files/ There are several other files there as well, mostly concerning IHC validation, including extensive references. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Chase Sent: Tuesday, August 28, 2012 6:44 AM To: 'Ian R Bernard'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Let's Talk CAP Inspections We just received the "All Common Checklist" all the questions from COM 40300 Analytic Accuracy/Precision to the end seem to be something for the clinical lab. How are some of you dealing with the following questions? COM.40300 Analytic Accuracy/Precision Phase II The laboratory verifies or establishes analytic accuracy and precision for each test. NOTE: Where current technology permits, accuracy is established by comparing results to a definitive or reference method, or may be verified by comparing results to an established comparative method. Use of reference materials or other materials with known concentrations or activities is suggested in establishing or verifying accuracy. Precision is established by repeat measurement of samples at varying concentrations or activities within-run and between-run over a period of time. Evidence of Compliance: ? Written procedure for determining method performance characteristics, including accuracy/precision AND ? Records of verification or establishment of analytic accuracy and precision for each test COM.40400 Analytic Sensitivity Phase II The laboratory verifies or establishes the analytic sensitivity (lower detection limit) of each assay, as applicable. NOTE: For FDA-cleared/approved tests, documentation may consist of data from manufacturers or the published literature. Evidence of Compliance: ? Written procedure for determining method performance characteristics, including analytic sensitivity AND ? Records of verification or establishment of analytic sensitivity for each assay COM.40500 Analytic Interferences Phase II The laboratory verifies or establishes analytic interferences for each test. NOTE: Interfering substances may pose a significant problem to the clinical laboratory and healthcare providers who may be misled by laboratory results that do not reflect patient clinical status. The laboratory must be aware of common interferences by performing studies or having available studies performed elsewhere (such as by the instrument-reagent manufacturer). Evidence of Compliance: ? Written procedure for determining method performance characteristics, including analytic interferences AND ? Records of verification or establishment of analytic interferences for each test COM.40600 Reportable Range Phase II The reportable range (analytic measurement range) is verified/established for each analytic procedure before implementation. NOTE: The analytic measurement range (AMR) is the range of analyte values that a method can directly measure on the specimen without any dilution or concentration. Expanded definitions and details of the AMR are provided in some of the section-specific checklists (e.g. Chemistry). Verification of the AMR may not apply to certain assays (for example, in immunology and coagulation). The limits of the AMR are based on meeting accuracy and precision requirements such as the minimal limit of quantification or sensitivity, when applicable. In some cases, clinically relevant limits may be narrower than the potential analytical range, and the clinically relevant limit would be used as the limit of the reportable range. Evidence of Compliance: ? Written policy for determining method performance characteristics, including reportable range AND ? Records of verification or establishment of reportable ranges for each test COM.40700 Method Performance Specifications Availability Phase II The laboratory's current test methods, including performance specifications and supporting validation data (analytic accuracy, precision, analytic sensitivity, interferences, reference range, and reportable range, as acceptable), are available to clients of the laboratory and to the inspection team upon request. NOTE: The laboratory must also provide data on clinical validity, if available, to clients upon request. The CAP inspection team is instructed to use the validation data solely for accreditation purposes. The laboratory may at its option require clients to agree to treat validation data as confidential and not to share such data with any other party except as required by law. COM.40800 Analytic Methodology Changes Phase II If the laboratory changes its analytic methodology so that test results or their interpretations may be SIGNIFICANTLY different, the change is explained to clients. NOTE: This requirement can be accomplished in any of several different ways, depending on local circumstances. Some methods include directed mailings, laboratory newsletters or part of the test report itself. Evidence of Compliance: ? Records such as directed mailings, laboratory newsletters or comment on the patient report advising of the change COM.50000 Reference Intervals Phase II The laboratory establishes or verifies its reference intervals (normal values). NOTE: Reference intervals are important to allow a clinician to assess patient results against an appropriate population. The reference range must be established or verified for each analyte and specimen source (e.g. blood, urine, cerebrospinal fluid), when appropriate. For many analytes (e.g. therapeutic drugs and CSF total protein), literature references or a manufacturer's package insert information may be appropriate. Evidence of Compliance: ? Record of reference range study OR records of verification of manufacturer's stated range when reference range study is not practical (e.g. unavailable normal population) OR other methods approved by the laboratory director COM.50100 Reference Interval Evaluation Phase II The laboratory evaluates the appropriateness of its reference intervals and takes corrective action if necessary. NOTE: Criteria for evaluation of reference intervals include: 1. Introduction of a new analyte to the test repertoire 2. Change of analytic methodology 3. Change in patient population If it is determined that the range is no longer appropriate for the patient population, corrective action must be taken. Evidence of Compliance: ? Records of evaluation and corrective action, if indicated -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Tuesday, August 28, 2012 6:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Let's Talk CAP Inspections I trust all of us will hopefully benefit from this line of discussion: In 2013 our lab will undergo our 2013 CAP inspection. The Cyto (Non-Gyn)-pathology, and Anatomic(Surgical only) Pathology and now the new "Commons" checklist will be used. Over the next weeks, in an effort to prepare, and to get feedback to CAP questions that tend to be subject-to-interpretation or subjective (since inspectors are our peers) versus objective questions (that are straight forward (or "elementary my dear friend"), I will present questions with hopes of obtaining interpretations from you, my fellow subject matter experts and fellow inspectors. By the way, this will be my first CAP inspection as sole manager of our lab. I also think volunteering to serve as an inspector will help the process as well. Look out, I may be coming your way. IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, August 28, 2012 4:37 AM To: 'angela smith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remote processor alarms We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. From histotech <@t> imagesbyhopper.com Tue Aug 28 19:26:43 2012 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Aug 28 19:26:55 2012 Subject: [Histonet] Open Position - Laboratory Supervisor - Pathology In-Reply-To: <761E2B5697F795489C8710BCC72141FF010322@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF010322@ex07.net.ucsf.edu> Message-ID: <6C023B94-751F-4717-AF18-63A4CB5F59DD@imagesbyhopper.com> Please see the following link for more information and online application. https://uhs.ats.hrsmart.com/cgi-bin/portal/highlightjob.cgi?jobid=75412 Manatee Memorial Hospital, a 319-bed acute care facility is located on the banks of the Manatee River in Bradenton, Florida. The hospital has been a major provider of quality healthcare in the Tampa Bay Region since 1953. Manatee Memorial Hospital has a medical staff of over 500 physicians and the only residency program in the county. The spectacular four-story patient tower welcomes visitors as they cross over the bridge from Palmetto to Bradenton. The hospital offers a comprehensive range of medical services, including 24-hour emergency services, cardiac, cardiovascular, surgery, outpatient services, women?s and children?s services and orthopedic services, in addition to specialty programs in chest pain, a primary stroke center and the only pediatric center and Level ll Neonatal Intensive Care Unit in the county. Manatee Memorial Hospital currently has a Supervisor- Laboratory Pathology position available. From kdboydhisto <@t> yahoo.com Wed Aug 29 08:29:47 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Aug 29 08:29:54 2012 Subject: [Histonet] cassette printers/slide printers/slide labelers Message-ID: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> ? I am looking for recommended brands, the pros/cons and cost advice on small scale cassette printers, slide printers or slide label systems for ?on demand? printing at the gross stations and at ?microtomes for tracking barcodes through the lab. NO software needed, these would have to interface into a new LIS system. ? Also, any advice/tips on yearly maintenance and consumable cost (cassettes, slides, labels, ink, print heads?..) would be appreciated!!!!!!! Vendors welcome to contact me by personal email. ? ? Thanks, ? Kelly From relia1 <@t> earthlink.net Wed Aug 29 08:31:00 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 29 08:31:09 2012 Subject: [Histonet] RELIA Histology Careers Bulletin 8/29/2012 Summer is almost over!! Let's Get the Ball Rolling -- The phone lines are open... Message-ID: <009b01cd85ea$87939510$96babf30$@earthlink.net> Hi Histonetters!! It is hard to believe the summer is almost over. The kids are going back to school and Labor Day is just a few days away! Before we know it will be Halloween, Thanksgiving, Christmas and even New Years Day!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2013? Are you a histo tech looking for More pay? Better hours? Better situation in general? Are you a histotech who wants to relocate to a new area? Are you a traveler considering transitioning into a permanent position? Are you a Histo Tech looking for an opportunity to advance into management? Let's Get The Ball Rolling!!! The Phone Lines are Open. If you answered YES to any of the above questions We Need to Chat!! My phone lines are open just for you - Wednesday 8/29 and Thursday 8/28 from 9am-7pm EST. You can reach me toll free at 866-607-3542 or on my cell phone at 407-353-5070. If those timeframes are inconvenient for you or if you would prefer to set up a specific time just shoot me an email with the time you would like to talk and the phone number you would like for me to use. If you know of anyone else who might be interested in subscribing to RELIA's Histology Careers Bulletin please feel free to pass this along to them. And if you refer someone and I place them I will pay you a referral fee. Here is some information about my current openings: IHC Tech - Dallas, TX Night Shift Histotech - Charlotte, NC Histotech PM evenings - Charlotte, NC Grossing Dermpath Histotech - Reading, PA Lead Histotech/Supervisor - Charlottesville, VA Dermpath Histotech - Atlanta, GA (Southside) Histology Tech - Hilton Head, SC Histology Tech - Portland, ME All of the opportunities that I represent are full time permanent positions with premier facilities throughout the United States. My clients offer competitive salaries, great benefits and in most cases relocation assistance. Remember it never hurts to look!!! Don't see any positions that catch your eye? No worries! I am getting new positions daily I could get your next job tomorrow so if you are looking at all Call Me!! Thanks, Pam 877-60 RELIA (877-607-3542) I know there are a lot of recruiters out there right now contacting you and your friends. RELIA is the only nationwide recruiting firm specializing in the permanent placement of histology professionals. Remember here at RELIA we work as your confidential advocate to help you make the move that is right for you when the time is right for you. Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From HornHV <@t> archildrens.org Wed Aug 29 09:00:54 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Aug 29 09:01:05 2012 Subject: [Histonet] cassette printers/slide printers/slide labelers In-Reply-To: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> References: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719BB4A17FD@EVS1.archildrens.org> I would love this same information. Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, August 29, 2012 8:30 AM To: histonet Subject: [Histonet] cassette printers/slide printers/slide labelers ? I am looking for recommended brands, the pros/cons and cost advice on small scale cassette printers, slide printers or slide label systems for ?on demand? printing at the gross stations and at ?microtomes for tracking barcodes through the lab. NO software needed, these would have to interface into a new LIS system. ? Also, any advice/tips on yearly maintenance and consumable cost (cassettes, slides, labels, ink, print heads?..) would be appreciated!!!!!!! Vendors welcome to contact me by personal email. ? ? Thanks, ? Kelly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kaclynch <@t> yahoo.com Wed Aug 29 10:12:00 2012 From: kaclynch <@t> yahoo.com (lynch kenia) Date: Wed Aug 29 10:12:06 2012 Subject: [Histonet] blade holder Message-ID: <1346253120.39334.YahooMailNeo@web112513.mail.gq1.yahoo.com> I am looking for a disposable blade holder for a Tissue-Tek cryostat model 4550. Yep, this is a pretty old model but there is an emotional attachment to it so we can't upgrade yet :) Anyway, if you happen to have one sitting around in storage, and are willing to sell us this part, please contact me. ? Thanks! ? Kenia Lynch, M.T./H.T Histology and Molecular Supervisor Caldwell Memorial Hospital Lenoir, NC From Rcartun <@t> harthosp.org Wed Aug 29 11:05:16 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 29 11:05:32 2012 Subject: [Histonet] Question regarding retrospective testing Message-ID: <503E057C.7400.0077.1@harthosp.org> I know that this had been discussed before, but I believe regulations have changed and I need to find out what other laboratories are currently doing when it comes to retrospective testing (histochemical stains, IHC, molecular testing, etc.) on cytology and surgical pathology specimens that have already been "signed-out". When can you add the result(s) and bill the test(s) to the original pathology report versus re-accessioning the specimen (with a new date-of-service) and reporting and billing under the new accession number? Thanks, Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From mpence <@t> grhs.net Wed Aug 29 11:13:31 2012 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Aug 29 11:13:37 2012 Subject: [Histonet] Question regarding retrospective testing In-Reply-To: <503E057C.7400.0077.1@harthosp.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974EA1@is-e2k3.grhs.net> I have never re-accessioned a case for this. I know that I have went back at least two years and billed a case for "additional studies". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2012 11:05 AM To: Histonet Subject: [Histonet] Question regarding retrospective testing I know that this had been discussed before, but I believe regulations have changed and I need to find out what other laboratories are currently doing when it comes to retrospective testing (histochemical stains, IHC, molecular testing, etc.) on cytology and surgical pathology specimens that have already been "signed-out". When can you add the result(s) and bill the test(s) to the original pathology report versus re-accessioning the specimen (with a new date-of-service) and reporting and billing under the new accession number? Thanks, Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Aug 29 11:43:32 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Aug 29 11:42:33 2012 Subject: [Histonet] Question regarding retrospective testing In-Reply-To: <503E057C.7400.0077.1@harthosp.org> References: <503E057C.7400.0077.1@harthosp.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0106B2@ex07.net.ucsf.edu> Rich, From 30 days after signout we require a new accession number or addendum (pathologist decide which to do depending on circumstances. Generally we prefer to make an addendum to the original report). For addendums, since we can't add new tests to the old accession number, the work is done under a non-clinical accession number (ie, research, teaching) and then the information is appended to the original report. The non-clinical number is just a work-around so we don't have a second clinical number to refer to. A second accession number is usually used if extensive testing outside histology is required, ie, molecular path. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2012 9:05 AM To: Histonet Subject: [Histonet] Question regarding retrospective testing I know that this had been discussed before, but I believe regulations have changed and I need to find out what other laboratories are currently doing when it comes to retrospective testing (histochemical stains, IHC, molecular testing, etc.) on cytology and surgical pathology specimens that have already been "signed-out". When can you add the result(s) and bill the test(s) to the original pathology report versus re-accessioning the specimen (with a new date-of-service) and reporting and billing under the new accession number? Thanks, Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Aug 29 11:44:21 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Aug 29 11:44:30 2012 Subject: [Histonet] Question regarding retrospective testing In-Reply-To: <503E057C.7400.0077.1@harthosp.org> References: <503E057C.7400.0077.1@harthosp.org> Message-ID: If I understand correctly....(and this is how we do it) A case is considered "archived" after 30 days. Within that 30 days, you bill the same account, which adds late charges. IF the case is pulled for a review to perform MOLECULAR testing, you can charge an 88363 for that review at any time for that review. (Our pathologists add an addendum stating Dr. So and So requested KRAS, EGFR - etc. which documents the charge.) After 30 days we have the case registered and the case is billed on the new registration if stains are done inhouse. Hope this makes sense... j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, August 29, 2012 12:05 PM To: Histonet Subject: [Histonet] Question regarding retrospective testing I know that this had been discussed before, but I believe regulations have changed and I need to find out what other laboratories are currently doing when it comes to retrospective testing (histochemical stains, IHC, molecular testing, etc.) on cytology and surgical pathology specimens that have already been "signed-out". When can you add the result(s) and bill the test(s) to the original pathology report versus re-accessioning the specimen (with a new date-of-service) and reporting and billing under the new accession number? Thanks, Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From doolee <@t> shands.ufl.edu Wed Aug 29 12:36:51 2012 From: doolee <@t> shands.ufl.edu (Dooley, Elaine O.) Date: Wed Aug 29 12:36:59 2012 Subject: [Histonet] histotech job Florida Message-ID: <6193C53146742E4586DD4838F47F79AE0D2D6A62@MSXMB02.Shands.local> Hello Histonetters, We are looking for a histologist for the day shift at Shands Teaching Hospital in Gainesville FL. We would like someone with an interest to learn immunohistochemistry or that has experience with immunohistochemistry. Please visit the Shands.org website to apply. You can also search under Shands Jobs. To look at the job description search requisition number 18335. Elaine Dooley Shands Teaching Hospital At the University of Florida Gainesville Florida Phone 352-265-0111 Ext 72117 From vtobias <@t> uw.edu Wed Aug 29 13:21:14 2012 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Wed Aug 29 13:21:39 2012 Subject: [Histonet] cassette printers/slide printers/slide labelers In-Reply-To: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> References: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> Message-ID: Kelly, The only small scale cassette printer I know of, is the one from Brady. We have a small facility that is using it with good results as far as I know. You don't print directly to the cassette. They are printing to a 1" x .25" chemically resistant label on a Zebra printer connected to the LIS. The label is then placed on the beveled surface of the cassette and attached using Brady's tool. Because Brady's tool destroys both ends of the label, we had to play with the size and alignment of the barcode and text. I don't know what the cost of the Brady instrument is, but it has to be way cheaper then any other offering. Does it need to use a proprietary cassette, I don't know. I only assisted with the LIS part of getting the label formatted correctly. Slides labels are being printed on chemically resistant labels from a Zebra printer as well. In our 2 larger facilities we use General Data cassette printers and slide printers vary. One uses Zebra printers and chemically resistant labels and the other uses Thermo Slide Mate printers with mixed results. When they are working good, they're great, but they can be temperamental. By the way, which LIS are you using? Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, August 29, 2012 6:30 AM To: histonet Subject: [Histonet] cassette printers/slide printers/slide labelers ? I am looking for recommended brands, the pros/cons and cost advice on small scale cassette printers, slide printers or slide label systems for "on demand" printing at the gross stations and at ?microtomes for tracking barcodes through the lab. NO software needed, these would have to interface into a new LIS system. ? Also, any advice/tips on yearly maintenance and consumable cost (cassettes, slides, labels, ink, print heads...) would be appreciated!!!!!!! Vendors welcome to contact me by personal email. ? ? Thanks, ? Kelly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Aug 29 13:44:19 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Aug 29 13:47:06 2012 Subject: [Histonet] cassette printers/slide printers/slide labelers In-Reply-To: References: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39163A794C3D@IBMB7Exchange.digestivespecialists.com> Kelly, I'm using the Signature Primera slide printer. It is small and works very well so far. It's distributed by Creative Waste Solutions http://cwsincorp.com/ And the web site to look at the printer is: http://www.primerahealthcare.com/signature-slide-printer.html Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Wednesday, August 29, 2012 2:21 PM To: 'Kelly Boyd'; 'histonet' Subject: RE: [Histonet] cassette printers/slide printers/slide labelers Kelly, The only small scale cassette printer I know of, is the one from Brady. We have a small facility that is using it with good results as far as I know. You don't print directly to the cassette. They are printing to a 1" x .25" chemically resistant label on a Zebra printer connected to the LIS. The label is then placed on the beveled surface of the cassette and attached using Brady's tool. Because Brady's tool destroys both ends of the label, we had to play with the size and alignment of the barcode and text. I don't know what the cost of the Brady instrument is, but it has to be way cheaper then any other offering. Does it need to use a proprietary cassette, I don't know. I only assisted with the LIS part of getting the label formatted correctly. Slides labels are being printed on chemically resistant labels from a Zebra printer as well. In our 2 larger facilities we use General Data cassette printers and slide printers vary. One uses Zebra printers and chemically resistant labels and the other uses Thermo Slide Mate printers with mixed results. When they are working good, they're great, but they can be temperamental. By the way, which LIS are you using? Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, August 29, 2012 6:30 AM To: histonet Subject: [Histonet] cassette printers/slide printers/slide labelers ? I am looking for recommended brands, the pros/cons and cost advice on small scale cassette printers, slide printers or slide label systems for "on demand" printing at the gross stations and at ?microtomes for tracking barcodes through the lab. NO software needed, these would have to interface into a new LIS system. ? Also, any advice/tips on yearly maintenance and consumable cost (cassettes, slides, labels, ink, print heads...) would be appreciated!!!!!!! Vendors welcome to contact me by personal email. ? ? Thanks, ? Kelly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Wed Aug 29 13:58:20 2012 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Aug 29 13:58:41 2012 Subject: [Histonet] cassette printers/slide printers/slide labelers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39163A794C3D@IBMB7Exchange.digestivespecialists.com> References: <1346246987.32255.YahooMailNeo@web141104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39163A794C3D@IBMB7Exchange.digestivespecialists.com> Message-ID: I use the Brady cassette printer. I like it. It cost somewhere around $5000 which includes the label printer and the attachment component. The printer will also print slide labels that are chemical resistant. The label printer is very versatile as Brady has many different sizes of labels. The lab recently decided that all reagents are to be labels exactly the same in all sections. They gave me paper labels that are not chemical resistant and have to be hand written and with my arthritis I can't do it but Brady has a label exactly the same size and I can print all the information. Roberta Horner HT/HTL Animal Diagnostic Lab The Pennsylvania State University From we3smitty <@t> yahoo.com Wed Aug 29 15:52:35 2012 From: we3smitty <@t> yahoo.com (angela smith) Date: Wed Aug 29 15:52:39 2012 Subject: [Histonet] peloris Message-ID: <1346273555.47173.YahooMailClassic@web125402.mail.ne1.yahoo.com> Has anyone out there had service issues with your peloris?? Breaking down a lot, throwing error codes?? I would like some information as at the moment we seem to be the only ones with issues.? Thank you Has anyone had a peloris replaced due to getting a lemon? From patrick.lewis <@t> seattlechildrens.org Wed Aug 29 16:36:57 2012 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Aug 29 16:37:07 2012 Subject: [Histonet] Can anyone recommend a good Rabbit Isotype Control antibody Message-ID: <3903BE18914F4440834F0E620415FFCA0BD38935@PPWEXD01B.childrens.sea.kids> Hi Everyone, Can anyone recommend a good Rabbit Isotype Control Antibody IgG1, to use as a negative control for my Polyclonal Rabbit antibodies. I am looking at Biocompare and GenTex has one for about $220.00 for 100 ug. GTX47478 But if someone has one they've used and works well, I'd be interested. Thanks Patrick, From BDeBrosse-Serra <@t> isisph.com Wed Aug 29 16:42:01 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Aug 29 16:42:11 2012 Subject: [Histonet] Can anyone recommend a good Rabbit Isotype Control antibody In-Reply-To: <3903BE18914F4440834F0E620415FFCA0BD38935@PPWEXD01B.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA0BD38935@PPWEXD01B.childrens.sea.kids> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092CE34BAC@EXCHMB01.isis.local> Jackson Immunoresearch cat. # 011-000-003 or from VWR cat # 100181-130. Much cheaper!!!! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, August 29, 2012 2:37 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Can anyone recommend a good Rabbit Isotype Control antibody Hi Everyone, Can anyone recommend a good Rabbit Isotype Control Antibody IgG1, to use as a negative control for my Polyclonal Rabbit antibodies. I am looking at Biocompare and GenTex has one for about $220.00 for 100 ug. GTX47478 But if someone has one they've used and works well, I'd be interested. Thanks Patrick, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Thu Aug 30 07:35:49 2012 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Thu Aug 30 07:35:53 2012 Subject: [Histonet] HT Position in Colorado Message-ID: <0E828EC51C7CC445A51E53F81B64E8C7027672@s-irv-exchmb.PathologyPartners.intranet> Great part time opportunities' for Histotechnician's in Centennial, Colorado! Colorado GI Pathology is looking for certified HT's or HTL's to join their existing laboratory staff. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a part time that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com From hans <@t> histologistics.com Thu Aug 30 08:37:08 2012 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Aug 30 08:37:16 2012 Subject: [Histonet] histomorphometrics done for bone samples Message-ID: Hello, I have a Dr. who is asking about how to get histomorphometrics done for bone samples, data needed is % of new bone present in samples from sites grafted with freeze-dried mineralized cancellous allograft bone. We can do the plastics on it but the computer assisted part, I do not know anything about. Thanks Hans B Snyder Histologistics 100 Barber ave Worcester, MA 01606 www.histologistics.com From ratliffjack <@t> hotmail.com Thu Aug 30 08:42:14 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Aug 30 08:42:23 2012 Subject: [Histonet] histomorphometrics done for bone samples In-Reply-To: References: Message-ID: Hello Hans! If you would like to discuss, please feel free to call me at your convenience. Best Regards, Jack Jack Ratliff Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Aug 30, 2012, at 8:37 AM, Hans B Snyder wrote: > Hello, > > I have a Dr. who is asking about how to get histomorphometrics done for > bone samples, data needed is % of new bone present in samples from sites > grafted with freeze-dried mineralized cancellous allograft bone. We can do > the plastics on it but the computer assisted part, I do not know anything > about. > > Thanks > > > Hans B Snyder > Histologistics > 100 Barber ave > Worcester, MA 01606 > www.histologistics.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KevinSeguin <@t> gmi-inc.com Thu Aug 30 08:50:05 2012 From: KevinSeguin <@t> gmi-inc.com (Kevin Seguin) Date: Thu Aug 30 08:50:11 2012 Subject: [Histonet] Pros and cons of Leica ST5050 slide stainer Message-ID: Hello All, Can someone help me with the pros and cons of using a Leica ST5050? Specifically, are there any quirks or inconveniences in using it? Thanks! Kevin Seguin kevinseguin@gmi-inc.com From Donna.Willis <@t> baylorhealth.edu Thu Aug 30 10:24:05 2012 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu Aug 30 10:24:12 2012 Subject: [Histonet] Fragmented Biopsy Samples Message-ID: <3FA597486B013249A2FC8EE113CBCC0219788AC664@BHDAEXVM33.bhcs.pvt> Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding. Don't want to use sponges. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Timothy.Morken <@t> ucsfmedctr.org Thu Aug 30 10:40:48 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Aug 30 10:39:44 2012 Subject: [Histonet] RE: Fragmented Biopsy Samples In-Reply-To: <3FA597486B013249A2FC8EE113CBCC0219788AC664@BHDAEXVM33.bhcs.pvt> References: <3FA597486B013249A2FC8EE113CBCC0219788AC664@BHDAEXVM33.bhcs.pvt> Message-ID: <761E2B5697F795489C8710BCC72141FF01097F@ex07.net.ucsf.edu> Donna, try the new SecureSette from ThermoFisher. They enclose the sample at grossing and do not require manipulation at embedding - just add wax and cool. Very interesting product. We tried them and they work well for some samples. However, they only accommodate straight needle bx of 2cm or less. Our prostate cores were 2.5 cm at times so it didn't quite fit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, August 30, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fragmented Biopsy Samples Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding. Don't want to use sponges. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Thu Aug 30 10:55:10 2012 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Aug 30 10:55:23 2012 Subject: [Histonet] RE: Fragmented Biopsy Samples In-Reply-To: <761E2B5697F795489C8710BCC72141FF01097F@ex07.net.ucsf.edu> References: <3FA597486B013249A2FC8EE113CBCC0219788AC664@BHDAEXVM33.bhcs.pvt> <761E2B5697F795489C8710BCC72141FF01097F@ex07.net.ucsf.edu> Message-ID: Try using histoscreen cassettes and that will eliminate the need for wrapping the tissue. If still insist on wrapping, ensure that they are dipping the tissue paper in formalin prior to putting the tissue on the paper. Also at embedding, if the tissue is stuck to the paper, take a blade and put it against the tissue and gently push to loosen the tissue, repeat this the length of the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, August 30, 2012 10:41 AM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Fragmented Biopsy Samples Donna, try the new SecureSette from ThermoFisher. They enclose the sample at grossing and do not require manipulation at embedding - just add wax and cool. Very interesting product. We tried them and they work well for some samples. However, they only accommodate straight needle bx of 2cm or less. Our prostate cores were 2.5 cm at times so it didn't quite fit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, August 30, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fragmented Biopsy Samples Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding. Don't want to use sponges. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From cbarone <@t> NEMOURS.ORG Thu Aug 30 14:41:56 2012 From: cbarone <@t> NEMOURS.ORG (Barone, Carol) Date: Thu Aug 30 14:41:57 2012 Subject: [Histonet] DAPI staining Message-ID: <2F06BE5D1D5D4F4991755CC53C0A4CBD1B3EBCD5@WLMMBS02.nemours.org> Someone sent me a question regarding DAPI staining ( I generally use Hoechst )...but anyway, they are having smearing of nuclear contents. I would guess that is from rupturing of the nuclear membrane....over fixation? Over air drying? They are fixing 10 mins in cold acetone and 10 mins to air dry. What do the experts out there say? This could be a learning moment for me , as well. From Gina.Rodriguez <@t> leica-microsystems.com Thu Aug 30 15:58:12 2012 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Thu Aug 30 15:58:32 2012 Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 09/05/2012) Message-ID: I am out of the office until 09/05/2012. I will respond to your message when I return. If you need immediate assistance please contact 800-248-0123 or Tech.support@leica-microsystems.com Note: This is an automated response to your message "Histonet Digest, Vol 105, Issue 35" sent on 8/30/2012 12:03:22 PM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From marktarango <@t> gmail.com Thu Aug 30 20:17:31 2012 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Aug 30 20:17:35 2012 Subject: [Histonet] DAPI staining In-Reply-To: <2F06BE5D1D5D4F4991755CC53C0A4CBD1B3EBCD5@WLMMBS02.nemours.org> References: <2F06BE5D1D5D4F4991755CC53C0A4CBD1B3EBCD5@WLMMBS02.nemours.org> Message-ID: Hi Carol, Does this person put them in a freezer for 20 mins or so after DAPI staining? Someone once told me this helps with smearing. I'm not really sure if its true, but couldn't hurt to try it. Mark On Thursday, August 30, 2012, Barone, Carol wrote: > Someone sent me a question regarding DAPI staining ( I generally use > Hoechst )...but anyway, they are having smearing of nuclear contents. I > would guess that is from rupturing of the nuclear membrane....over > fixation? Over air drying? They are fixing 10 mins in cold acetone and 10 > mins to air dry. What do the experts out there say? This could be a > learning moment for me , as well. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Philip.Gibson <@t> nuth.nhs.uk Fri Aug 31 02:33:28 2012 From: Philip.Gibson <@t> nuth.nhs.uk (Gibson, Philip) Date: Fri Aug 31 02:34:21 2012 Subject: [Histonet] Peloris problems Message-ID: <20120831073330.13D9C4482A5@nhs-pd1e-esg107.ad1.nhs.net> Hi Angela Our lab has repeatedly had problems with the Leica Peloris! Fortunately (for Leica) the Peloris processes tissues very well, so our lab has persisted with our two machines for around 5 years now. I believe there is now a "version 2" machine on the market that corrects many of the niggles (at one point we had AT LEAST one processor crash every week!), and one of our machines was fitted with the updated parts. Currently, the processors are running more reliably, partly because Leica have "forced" us into running longer, cooler wax impregnation steps that have resulted in a minimum 15 hour long overnight processing schedule! This has been very bad for our workflow, but apparently it is stopping the IPA from foaming too much and blocking air lines! Our only other frustration is the dumb reagent management software which has an uncanny knack of trying to use the same solutions for the two retorts, when there are clearly 2 brand new pure solutions available to use! It's very frustrating running what (after evaluating several manufacturers rival products) seems to be by far the best processor on the market, but one that utterly fails at being reliable! We also run Shandon pathcentres (poor) and a Tissue Tek VIP (reliably competent). I'll warn that this is all very much my own personal opinion!!! Regards Phil Date: Wed, 29 Aug 2012 13:52:35 -0700 (PDT) From: angela smith Subject: [Histonet] peloris To: histonet@lists.utsouthwestern.edu Message-ID: <1346273555.47173.YahooMailClassic@web125402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has anyone out there had service issues with your peloris? Breaking down a lot, throwing error codes? I would like some information as at the moment we seem to be the only ones with issues. Thank you Has anyone had a peloris replaced due to getting a lemon? ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ******************************************************************************************************************** This email has been processed by SmoothZap - www.smoothwall.net From gayle.callis <@t> bresnan.net Fri Aug 31 11:23:05 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Aug 31 11:23:26 2012 Subject: [Histonet] Re: DAPI stained smeared nuclei Message-ID: <000001cd8794$e7ff4bf0$b7fde3d0$@bresnan.net> Carol wrote: Someone sent me a question regarding DAPI staining ( I generally use Hoechst )...but anyway, they are having smearing of nuclear contents. I would guess that is from rupturing of the nuclear membrane....over fixation? Over air drying? They are fixing 10 mins in cold acetone and 10 mins to air dry. What do the experts out there say? This could be a learning moment for me, as well. **************************************************************************** ************** Interesting problem, but I have never had problems with DAPI staining after either 4C acetone or my beloved acetone/alcohol fixation for murine CD markers and other antigens that like solvent fixatives. No problems occur after air drying either. We always air dry our frozen sections very well before fixation and/or storage at -80C. I don't think one can over air dry unless they leave the sections out for days at RT, then antigenicity could be damaged. I suspect there is something else going on with either how they store frozen sections after sectioning rather than air drying, fixation in cold acetone and then air drying again. Possibly doing some kind of damaging thaw, freeze, thaw? Storing frozens in cryostat immediately after sectioning is not advisable since condensation forms on FS when one takes them out of cold environment. How do they pick up the sections as I can smear a frozen section quite thoroughly if I am not quick and tidy when mounting the FS on a slide. The tiniest movement will mess up tissue components, even nuclei The very act of picking up a section on a slide can be referred to as flash drying and is, in some ways a means of fixation although not very good. More details would help on HOW they handle the sections before fixation? What tissues? Cutting temperatures? What is actually happening to their immunofluorescence staining? Is that fine but the nuclei are smeared? The only problem we ever had with DAPI staining is using mounting media with DAPI which gave a staining gradient with dim staining of the nuclei in certain areas of the tissue section. We solved this by never using mounting media with DAPI and now buy DAPI solution from Biogenex or Pierce, then stain with DAPI solution AFTER all the immunofluorescence staining was complete followed by Prolong Gold Antifade reagent. What kind of mounting media do they use? How do they rinse after all the IF staining? Do they use an in house prepared DAPI solution and if so, is it made correctly? Mark Tarango brought up an interesting solution to the problem, but what happens after you pull the sections from freezer and go back to RT? Tossing out a lot of things here......................... Take care Gayle Callis HTL/HT/MT(ASCP) Bozeman MT From carl.hobbs <@t> kcl.ac.uk Fri Aug 31 13:44:45 2012 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Aug 31 13:44:57 2012 Subject: [Histonet] Re: DAPI stained smeared nuclei Message-ID: <00D6B8253EAED840B8D04E2354973818012652@AMSPRD0310MB374.eurprd03.prod.outlook.com> When you use "smear", do you mean that the nuclei are blurring/ill-defined , with threads of DAPI-positivity coming away from the nuclei? If so, it is due to underfixation. Acetone is not a proper fixative. In the time one uses is, it rapidly delipidises the tissue, rendering it better preserved, not fixed. So, occassionally the nuclei "unravel". A mixture of acetone: methanol overcomes this by coagulating nucleoproteins. Sure, many times one does not notice significant "smearing" after acetone fixation. No difference, in practise, between DAPI and Hoechst. I use Hoechst: at a dilution factor of 1/20K it lasts quite a long time, lol. Conservatorily, Carl From TNMayer <@t> mdanderson.org Fri Aug 31 14:25:56 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Aug 31 14:26:32 2012 Subject: [Histonet] RE: Peloris problems Message-ID: I used the Peloris at Labcorp for about 2 years. I liked it a lot. It tells you when the reagents are ready to be changed, and can do two separate runs simultaneously. Like any machine it has drawbacks such as reagent competition and no it doesn't tell you how to correct a problem. One time we were adding paraffin and had that door open, the machine would not start until the paraffin door was closed, unlike the VIP. It didn't even tell me to close the door. I know that sounds silly (I should have known to do that), but I was adding cold paraffin and did not want to forget to check the level before I left, so it was open. We all do that. The machine was running during Hurricane Ike in Houston, and of course when the power went off it locked up. After the repair guy came out and serviced it, it ran like a little workhorse. We usually ran both chambers overnight and one chamber during the day. We even had a run come out on Saturday, and ran it again to come out Monday. Service was good and prompt, and Leica came and updated the software when needed. Because we ran it so much something went wrong every quarter or so, but was not down for more than a day. I chalked that up to being new and in pretty heavy rotation. Hope this helps. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 4 Date: Fri, 31 Aug 2012 08:33:28 +0100 From: "Gibson, Philip" Subject: [Histonet] Peloris problems To: Message-ID: <20120831073330.13D9C4482A5@nhs-pd1e-esg107.ad1.nhs.net> Content-Type: text/plain; charset="us-ascii" Hi Angela Our lab has repeatedly had problems with the Leica Peloris! Fortunately (for Leica) the Peloris processes tissues very well, so our lab has persisted with our two machines for around 5 years now. I believe there is now a "version 2" machine on the market that corrects many of the niggles (at one point we had AT LEAST one processor crash every week!), and one of our machines was fitted with the updated parts. Currently, the processors are running more reliably, partly because Leica have "forced" us into running longer, cooler wax impregnation steps that have resulted in a minimum 15 hour long overnight processing schedule! This has been very bad for our workflow, but apparently it is stopping the IPA from foaming too much and blocking air lines! Our only other frustration is the dumb reagent management software which has an uncanny knack of trying to use the same solutions for the two retorts, when there are clearly 2 brand new pure solutions available to use! It's very frustrating running what (after evaluating several manufacturers rival products) seems to be by far the best processor on the market, but one that utterly fails at being reliable! We also run Shandon pathcentres (poor) and a Tissue Tek VIP (reliably competent). I'll warn that this is all very much my own personal opinion!!! Regards Phil Date: Wed, 29 Aug 2012 13:52:35 -0700 (PDT) From: angela smith Subject: [Histonet] peloris To: histonet@lists.utsouthwestern.edu Message-ID: <1346273555.47173.YahooMailClassic@web125402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has anyone out there had service issues with your peloris? Breaking down a lot, throwing error codes? I would like some information as at the moment we seem to be the only ones with issues. Thank you Has anyone had a peloris replaced due to getting a lemon? ---------------------------------------- Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 From cbarone <@t> NEMOURS.ORG Fri Aug 31 15:33:30 2012 From: cbarone <@t> NEMOURS.ORG (Barone, Carol) Date: Fri Aug 31 15:33:31 2012 Subject: [Histonet] DAPI Message-ID: <2F06BE5D1D5D4F4991755CC53C0A4CBD1B3EBF71@WLMMBS02.nemours.org> Sorry I don't not have more info on the DAPI user. Unfortunately...or rather fortunately....I have never personally had this problem. This is a corporate user, we have done some H&E's for, on FS with no issues. This is just not a problem I have ever had. I will pass these on to him; they are interesting in-sites! I love to learn anything new....Thx Mark and Gayle.