[Histonet] proliferation and apoptosis

[Mehlika Faire]

I've used pHH3 to mark proliferative cells and it works pretty well.  Active-caspase-3 and cleaved-parp  have worked well for staining apoptotic cells as well.
-Mehlika

> From: liz <@t> premierlab.com
> To: b427297 <@t> aol.com; NMargaryan <@t> childrensmemorial.org; histonet-request <@t> lists.utsouthwestern.edu
> Date: Thu, 19 Apr 2012 15:37:26 -0600
> Subject: RE: [Histonet] proliferation and apoptosis
> CC: histonet <@t> lists.utsouthwestern.edu
> 
> I agree with Jackie, we use Ki-67 all of the time and I have never seen it stain apoptotic cells, could you possibly be dealing with some background staining due to the detection system used?  I do not know what type of samples you are staining?  We have 4 different Ki-67 antibodies we use depending upon the type of tissue we are staining.  For human xenograft samples in a mouse background we use the rabbit polyclonal from Santa Cruz.  We also use cleaved caspase 3 as a marker of apoptosis routinely.
> 
> Liz
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308-1592
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> www.premierlab.com
> 
> Ship to address:
> 
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor
> Sent: Thursday, April 19, 2012 3:23 PM
> To: NMargaryan <@t> childrensmemorial.org; histonet-request <@t> lists.utsouthwestern.edu
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] proliferation and apoptosis
> 
> 
> Ki67 does not stain apoptotic cells. Why do you have that impression?  Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation.   I've used Ki67 for proliferation  and Caspase 3 for apoptosis routinely in cancer research for years.
> 
> Jackie
> 
> 
> -----Original Message-----
> From: Margaryan, Naira <NMargaryan <@t> childrensmemorial.org>
> To: histonet-request <histonet-request <@t> lists.utsouthwestern.edu>
> Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thu, Apr 19, 2012 3:09 pm
> Subject: [Histonet] proliferation and apoptosis
> 
> 
> Hi histonetters,
> 
> I am looking for the good markers to detect (separately) proliferation and
> apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic
> bodies as well as proliferated cells; and the tunnel assay shows both apoptotic
> body and proliferation.
> 
> Any suggestions for the FFPE tissue and the HRP protocol are appreciated.
> 
> Thanks in advance,
> Naira
> 
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